Cla97C09G169430 (gene) Watermelon (97103) v2.5

Overview
NameCla97C09G169430
Typegene
OrganismCitrullus lanatus subsp. vulgaris cv. 97103 (Watermelon (97103) v2.5)
Descriptionisoamylase 2, chloroplastic
LocationCla97Chr09: 5687766 .. 5691415 (-)
RNA-Seq ExpressionCla97C09G169430
SyntenyCla97C09G169430
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: polypeptideexonCDS
Hold the cursor over a type above to highlight its positions in the sequence below.
ATGCTGGTGGGATCCTCACAAGAAGACGAAGCAGAGGTACAGAAACTCGCTCTCCTTCGCTCCTGTCCCTCTCCAACACTCCATCTCCATCGCAGACCTTCCATTTGCGGTGCTTTGATTTCGATTATTTATTACCATAAAGAACAACCCACATTCTCCTCTCCTTCGTCTCTAATTCCTTTTCTTTTCTGTCCATCAGCACTGCGAGCAGAATCAAAGAAATGTAAGTTCAAATTCTCCAACTCTGCTTTTGAGCTATATTTCTCAATCTTTTGAAGCTTTTTCCATCTGGGTTTCGCTCAATTTTGATTTCTGATGACTTTCTCTTTCTGTCCTGAGCTCTTGATTCACACAAAAATGGATTGATTAGGTTATATTCAATGTACATGCTGATTATGGTTAAAATATCGATGTCTACGGAAATGTAGAGATCTTGTCAATGAAAATGTCGCAAAATTATAAAAAGAAATTTAAATAAATTATTCAAATTAATAAATAAACATTTCATGCTTTTTAAAAAAGTTTAAATGTTTATTATCTATATTATATTCACATTTGTAATATTTTGTTGCTTATCTTTTATATTTCATTGAGCTTTTCACGATATAATGGAAATTGTGAAAATGTCAATGAAAATGTTGATATGTTAGATGAAAATTTAATATCATGATTCTAATGTAATTGGATATTAATTTGATTCATGAAGTCTAACAAGTGAACTGATAGCATTGGTCGAGGAGAAAATGACATTGCAGTGAATTAATGTAGCATGGAAAGACTTCATGAACCAACTAACTAAAGAGGATTGGTTAGAATGTAGTTAATATTATACACGTATTCTTAGATCCTCTATGTTTATTTGATGGCCACTGTTGCATTTAGTCTAGGAGCTGAACAGAATTGATGGTTTATTCTTGATTGGCACAGAACAATTGATCAATTTCAGGTTGGAAGGTTTCTTTCTTTTCAGTTAGATGCTTTTGCAGTTATAGATGGCAACTTTCCCGTCTTCACTTGTCATACAGCCCTTCTGTGTATATAATTGTGGAGTTACTGAATCATTCAAACTGGCTGTAAGTGATCACCATACATATGGACAGAAGATAAAGTATCAGTCTGGAAAAATGGATGAGGCTAGAATGCTAGCTCATGGAGAACGTAAAGTAAGTTTTATGAAATCTTCGCACCGCAACCTTAGTATAGCATATGCTACATCGGGAATTTCGGTTGGGAAAAGTGGACAGAGATTTAGTACTGGTGGTAAAAGTAAGGAACACAGAAAGGTTGCAACTTATCTGTTTAGAACAGAATTTGGGGATTTGGTGAATGTTTTTGTTGAAAAGACGGGGTCTGGTTTTACAGTCAATATCGAAATTTCGTCCGTGCAACGAGCAAACATTGATGAAACACTGCTGCTTAGTTGGGGGGTTTATAGATCTGATTCAACTCTTGTAACTCCCAACTTTCAGAGTTCACTCCCAGATGAGACAATTGGAGCCACTGAAACCCCTTTTTTGAAAACTTCTGAAGGAAAATTTGCTGTTGAATTGGAGTTTGATGCTAAGCACACTCCTTTCTACCTTTCTTTTCTGTTGAAATATTCATTGGGTGTTGATTCAGGCAGCTCTGAAATTAGAAGTCACAGGAAGACTAGCTTTCCTGTACCTGTTGGATTTGGTCGAGGCTATCCGAGTCCGTTGGGTCTTTCCATTTCAGGTGATGGTTCCATAAATTTTTCCATTTTCTCAAGCACTGCAGAAAGTGTAGTTTTATGTCTGTACAATGACAGCACATCAGAAAAACCTGTATTAGAGCTAGATTTAGATCCATACATTAACAAATCAGGTAATATTTGGCATGCCTCATTTGAAGGTGCCTCAAAATTTGTAAGCTATGGTTATCGATGCAAGGGGTCCAAGTTTCATGAAAATCAAGATGGAGTTGAGGTAAGTCAGATTGTTGTGGATCCGTATGCTAAGATCCTCACAGCATCTATTCCTAAAGCTCGTGGTCAGGGACTTGGATTGCCTTCAAATCTTCTTGGGCAAATATGCAAAGTACCTGCCTTTGATTGGGATGGCGAAGTCCGTCCTAACTTGCCAATGGAGAAACTTTTTGTTTATCGGTTGAATGTGAAGCATTTTACCATCGACAAATCAAGTCAGCTTCCGGCAGATATTGCTGGTACATTTTCTGGTTTGACCAAGAAATTGTTGCATTTTAAAAACTTGGGAGTGAATGCAGTTCAACTAGAGCCAATCTTTCAATTTGATGAGAAAGAAGGACCATATTTTCCCTTTCACTTCTTTTCTCCAACAAATAGTTATGGTCCTTCTGGGGCTTCCATTTCTGTTATCAACTCCATGAAGGAAATGGTTAAAGAACTGCATGCCAATGGAATAGAGGTCATCTTGGAAGTTGTTTTTACTCACACTGCTGTGGATGGAGCACTGCAAGGGATTGATGATTCGTCTTACTATTTTGTGAACAGGGTTGCGAATTTGGAAGGAAAAAGTGTTCTGAACTGCAATTATCCCATAGTGCAACAATTACTTTTGGATAGTCTTCGATATTGGGTGACTGAGTTTCATATAGATGGTTTTTGTTTCATAAATGCTTCATCTTTGCTGCGAGGACATCATGGCGAATTGTTATCTCGTCCTCCTTTTGTAGAAGCGGTTGCTTTTGATCCGCTTCTATCAAAAACTAAACTAGTTGCAGACTTCTGGGATCCACAGGATTTAGTATCAAAGGAAACTCATTTTCCTCATTGGAAGAGATGGGCAGAGGTGAATTCAAAGTTCTGTAATGATATAAGGGACTTTTTCAGGGGTGAAGGTCTTATCAGTAGCCTTGCAACCCGACTTTGTGGGAGCGGAGATGTATTCTCAGATGGTCGAGGCCCTGCATTCTCTTTCAATTTCATTGCTAGAAACGTTGGCCTTCCTCTCGTCGACCTTGTGAGCTTTAGCGACAGCAAGTTAGCTTCAGAGTTGAGTTCGAATTGTGGGGAAGAAGGACCGACAAGTAATATTGAAGTTCTAGAAAAACGACTCAAACAAATTCGTAATTTTATATTTGTGTTGTTTGTTTCACTTGGTGTTCCTGTTCTTAACATGGGAGACGAGTGCGGCCAATCGTCAGGGGGATCTGTGGCATCCAACGACAGGAGGTCATTCAATTGGGATTTACTGAAGACAGATTTCGGTACTCAAACCACACAATTCATTGCATTTTTTAGTTCATTTAGATCAAGGCGTTTTGACCTTTTCCAGAGCAGAAACTTTCTGAAGGGAGAGAACATAGACTGGTTTGATAATAACCAATCATCACCACAGTGGGAAGATCCTTCTTGCAAATTCCTGGCTGTGATGCTGAGGGCTGACAAAGAGGAAAATGGATCAATCACTGAGAATGCCAAGCCTCGAAGCGATATATTTATGGCTTTCAATGCATCTGACCAATCTGAAAGTGTTGCTTTACCTGAACCATCAGAAGGTACATCCTGGTTCAGGCTGGTTGACACAGCTCTTTCTTTTCCCGGGTTTTTCTCATTCAATGGCGAGCTGGTGCCAACGACAGGATCTGTTACTTATGAAATGCAAGCTCATAGTTGTGCACTTTTTGAGGCTAAAAGTTCTAATGATTGA

mRNA sequence

ATGCTGATGGCAACTTTCCCGTCTTCACTTGTCATACAGCCCTTCTGTGTATATAATTGTGGAGTTACTGAATCATTCAAACTGGCTGTAAGTGATCACCATACATATGGACAGAAGATAAAGTATCAGTCTGGAAAAATGGATGAGGCTAGAATGCTAGCTCATGGAGAACGTAAAGTAAGTTTTATGAAATCTTCGCACCGCAACCTTAGTATAGCATATGCTACATCGGGAATTTCGGTTGGGAAAAGTGGACAGAGATTTAGTACTGGTGGTAAAAGTAAGGAACACAGAAAGGTTGCAACTTATCTGTTTAGAACAGAATTTGGGGATTTGGTGAATGTTTTTGTTGAAAAGACGGGGTCTGGTTTTACAGTCAATATCGAAATTTCGTCCGTGCAACGAGCAAACATTGATGAAACACTGCTGCTTAGTTGGGGGGTTTATAGATCTGATTCAACTCTTGTAACTCCCAACTTTCAGAGTTCACTCCCAGATGAGACAATTGGAGCCACTGAAACCCCTTTTTTGAAAACTTCTGAAGGAAAATTTGCTGTTGAATTGGAGTTTGATGCTAAGCACACTCCTTTCTACCTTTCTTTTCTGTTGAAATATTCATTGGGTGTTGATTCAGGCAGCTCTGAAATTAGAAGTCACAGGAAGACTAGCTTTCCTGTACCTGTTGGATTTGGTCGAGGCTATCCGAGTCCGTTGGGTCTTTCCATTTCAGGTGATGGTTCCATAAATTTTTCCATTTTCTCAAGCACTGCAGAAAGTGTAGTTTTATGTCTGTACAATGACAGCACATCAGAAAAACCTGTATTAGAGCTAGATTTAGATCCATACATTAACAAATCAGGTAATATTTGGCATGCCTCATTTGAAGGTGCCTCAAAATTTGTAAGCTATGGTTATCGATGCAAGGGGTCCAAGTTTCATGAAAATCAAGATGGAGTTGAGGTAAGTCAGATTGTTGTGGATCCGTATGCTAAGATCCTCACAGCATCTATTCCTAAAGCTCGTGGTCAGGGACTTGGATTGCCTTCAAATCTTCTTGGGCAAATATGCAAAGTACCTGCCTTTGATTGGGATGGCGAAGTCCGTCCTAACTTGCCAATGGAGAAACTTTTTGTTTATCGGTTGAATGTGAAGCATTTTACCATCGACAAATCAAGTCAGCTTCCGGCAGATATTGCTGGTACATTTTCTGGTTTGACCAAGAAATTGTTGCATTTTAAAAACTTGGGAGTGAATGCAGTTCAACTAGAGCCAATCTTTCAATTTGATGAGAAAGAAGGACCATATTTTCCCTTTCACTTCTTTTCTCCAACAAATAGTTATGGTCCTTCTGGGGCTTCCATTTCTGTTATCAACTCCATGAAGGAAATGGTTAAAGAACTGCATGCCAATGGAATAGAGGTCATCTTGGAAGTTGTTTTTACTCACACTGCTGTGGATGGAGCACTGCAAGGGATTGATGATTCGTCTTACTATTTTGTGAACAGGGTTGCGAATTTGGAAGGAAAAAGTGTTCTGAACTGCAATTATCCCATAGTGCAACAATTACTTTTGGATAGTCTTCGATATTGGGTGACTGAGTTTCATATAGATGGTTTTTGTTTCATAAATGCTTCATCTTTGCTGCGAGGACATCATGGCGAATTGTTATCTCGTCCTCCTTTTGTAGAAGCGGTTGCTTTTGATCCGCTTCTATCAAAAACTAAACTAGTTGCAGACTTCTGGGATCCACAGGATTTAGTATCAAAGGAAACTCATTTTCCTCATTGGAAGAGATGGGCAGAGGTGAATTCAAAGTTCTGTAATGATATAAGGGACTTTTTCAGGGGTGAAGGTCTTATCAGTAGCCTTGCAACCCGACTTTGTGGGAGCGGAGATGTATTCTCAGATGGTCGAGGCCCTGCATTCTCTTTCAATTTCATTGCTAGAAACGTTGGCCTTCCTCTCGTCGACCTTGTGAGCTTTAGCGACAGCAAGTTAGCTTCAGAGTTGAGTTCGAATTGTGGGGAAGAAGGACCGACAAGTAATATTGAAGTTCTAGAAAAACGACTCAAACAAATTCGTAATTTTATATTTGTGTTGTTTGTTTCACTTGGTGTTCCTGTTCTTAACATGGGAGACGAGTGCGGCCAATCGTCAGGGGGATCTGTGGCATCCAACGACAGGAGGTCATTCAATTGGGATTTACTGAAGACAGATTTCGGTACTCAAACCACACAATTCATTGCATTTTTTAGTTCATTTAGATCAAGGCGTTTTGACCTTTTCCAGAGCAGAAACTTTCTGAAGGGAGAGAACATAGACTGGTTTGATAATAACCAATCATCACCACAGTGGGAAGATCCTTCTTGCAAATTCCTGGCTGTGATGCTGAGGGCTGACAAAGAGGAAAATGGATCAATCACTGAGAATGCCAAGCCTCGAAGCGATATATTTATGGCTTTCAATGCATCTGACCAATCTGAAAGTGTTGCTTTACCTGAACCATCAGAAGGTACATCCTGGTTCAGGCTGGTTGACACAGCTCTTTCTTTTCCCGGGTTTTTCTCATTCAATGGCGAGCTGGTGCCAACGACAGGATCTGTTACTTATGAAATGCAAGCTCATAGTTGTGCACTTTTTGAGGCTAAAAGTTCTAATGATTGA

Coding sequence (CDS)

ATGCTGATGGCAACTTTCCCGTCTTCACTTGTCATACAGCCCTTCTGTGTATATAATTGTGGAGTTACTGAATCATTCAAACTGGCTGTAAGTGATCACCATACATATGGACAGAAGATAAAGTATCAGTCTGGAAAAATGGATGAGGCTAGAATGCTAGCTCATGGAGAACGTAAAGTAAGTTTTATGAAATCTTCGCACCGCAACCTTAGTATAGCATATGCTACATCGGGAATTTCGGTTGGGAAAAGTGGACAGAGATTTAGTACTGGTGGTAAAAGTAAGGAACACAGAAAGGTTGCAACTTATCTGTTTAGAACAGAATTTGGGGATTTGGTGAATGTTTTTGTTGAAAAGACGGGGTCTGGTTTTACAGTCAATATCGAAATTTCGTCCGTGCAACGAGCAAACATTGATGAAACACTGCTGCTTAGTTGGGGGGTTTATAGATCTGATTCAACTCTTGTAACTCCCAACTTTCAGAGTTCACTCCCAGATGAGACAATTGGAGCCACTGAAACCCCTTTTTTGAAAACTTCTGAAGGAAAATTTGCTGTTGAATTGGAGTTTGATGCTAAGCACACTCCTTTCTACCTTTCTTTTCTGTTGAAATATTCATTGGGTGTTGATTCAGGCAGCTCTGAAATTAGAAGTCACAGGAAGACTAGCTTTCCTGTACCTGTTGGATTTGGTCGAGGCTATCCGAGTCCGTTGGGTCTTTCCATTTCAGGTGATGGTTCCATAAATTTTTCCATTTTCTCAAGCACTGCAGAAAGTGTAGTTTTATGTCTGTACAATGACAGCACATCAGAAAAACCTGTATTAGAGCTAGATTTAGATCCATACATTAACAAATCAGGTAATATTTGGCATGCCTCATTTGAAGGTGCCTCAAAATTTGTAAGCTATGGTTATCGATGCAAGGGGTCCAAGTTTCATGAAAATCAAGATGGAGTTGAGGTAAGTCAGATTGTTGTGGATCCGTATGCTAAGATCCTCACAGCATCTATTCCTAAAGCTCGTGGTCAGGGACTTGGATTGCCTTCAAATCTTCTTGGGCAAATATGCAAAGTACCTGCCTTTGATTGGGATGGCGAAGTCCGTCCTAACTTGCCAATGGAGAAACTTTTTGTTTATCGGTTGAATGTGAAGCATTTTACCATCGACAAATCAAGTCAGCTTCCGGCAGATATTGCTGGTACATTTTCTGGTTTGACCAAGAAATTGTTGCATTTTAAAAACTTGGGAGTGAATGCAGTTCAACTAGAGCCAATCTTTCAATTTGATGAGAAAGAAGGACCATATTTTCCCTTTCACTTCTTTTCTCCAACAAATAGTTATGGTCCTTCTGGGGCTTCCATTTCTGTTATCAACTCCATGAAGGAAATGGTTAAAGAACTGCATGCCAATGGAATAGAGGTCATCTTGGAAGTTGTTTTTACTCACACTGCTGTGGATGGAGCACTGCAAGGGATTGATGATTCGTCTTACTATTTTGTGAACAGGGTTGCGAATTTGGAAGGAAAAAGTGTTCTGAACTGCAATTATCCCATAGTGCAACAATTACTTTTGGATAGTCTTCGATATTGGGTGACTGAGTTTCATATAGATGGTTTTTGTTTCATAAATGCTTCATCTTTGCTGCGAGGACATCATGGCGAATTGTTATCTCGTCCTCCTTTTGTAGAAGCGGTTGCTTTTGATCCGCTTCTATCAAAAACTAAACTAGTTGCAGACTTCTGGGATCCACAGGATTTAGTATCAAAGGAAACTCATTTTCCTCATTGGAAGAGATGGGCAGAGGTGAATTCAAAGTTCTGTAATGATATAAGGGACTTTTTCAGGGGTGAAGGTCTTATCAGTAGCCTTGCAACCCGACTTTGTGGGAGCGGAGATGTATTCTCAGATGGTCGAGGCCCTGCATTCTCTTTCAATTTCATTGCTAGAAACGTTGGCCTTCCTCTCGTCGACCTTGTGAGCTTTAGCGACAGCAAGTTAGCTTCAGAGTTGAGTTCGAATTGTGGGGAAGAAGGACCGACAAGTAATATTGAAGTTCTAGAAAAACGACTCAAACAAATTCGTAATTTTATATTTGTGTTGTTTGTTTCACTTGGTGTTCCTGTTCTTAACATGGGAGACGAGTGCGGCCAATCGTCAGGGGGATCTGTGGCATCCAACGACAGGAGGTCATTCAATTGGGATTTACTGAAGACAGATTTCGGTACTCAAACCACACAATTCATTGCATTTTTTAGTTCATTTAGATCAAGGCGTTTTGACCTTTTCCAGAGCAGAAACTTTCTGAAGGGAGAGAACATAGACTGGTTTGATAATAACCAATCATCACCACAGTGGGAAGATCCTTCTTGCAAATTCCTGGCTGTGATGCTGAGGGCTGACAAAGAGGAAAATGGATCAATCACTGAGAATGCCAAGCCTCGAAGCGATATATTTATGGCTTTCAATGCATCTGACCAATCTGAAAGTGTTGCTTTACCTGAACCATCAGAAGGTACATCCTGGTTCAGGCTGGTTGACACAGCTCTTTCTTTTCCCGGGTTTTTCTCATTCAATGGCGAGCTGGTGCCAACGACAGGATCTGTTACTTATGAAATGCAAGCTCATAGTTGTGCACTTTTTGAGGCTAAAAGTTCTAATGATTGA

Protein sequence

MLMATFPSSLVIQPFCVYNCGVTESFKLAVSDHHTYGQKIKYQSGKMDEARMLAHGERKVSFMKSSHRNLSIAYATSGISVGKSGQRFSTGGKSKEHRKVATYLFRTEFGDLVNVFVEKTGSGFTVNIEISSVQRANIDETLLLSWGVYRSDSTLVTPNFQSSLPDETIGATETPFLKTSEGKFAVELEFDAKHTPFYLSFLLKYSLGVDSGSSEIRSHRKTSFPVPVGFGRGYPSPLGLSISGDGSINFSIFSSTAESVVLCLYNDSTSEKPVLELDLDPYINKSGNIWHASFEGASKFVSYGYRCKGSKFHENQDGVEVSQIVVDPYAKILTASIPKARGQGLGLPSNLLGQICKVPAFDWDGEVRPNLPMEKLFVYRLNVKHFTIDKSSQLPADIAGTFSGLTKKLLHFKNLGVNAVQLEPIFQFDEKEGPYFPFHFFSPTNSYGPSGASISVINSMKEMVKELHANGIEVILEVVFTHTAVDGALQGIDDSSYYFVNRVANLEGKSVLNCNYPIVQQLLLDSLRYWVTEFHIDGFCFINASSLLRGHHGELLSRPPFVEAVAFDPLLSKTKLVADFWDPQDLVSKETHFPHWKRWAEVNSKFCNDIRDFFRGEGLISSLATRLCGSGDVFSDGRGPAFSFNFIARNVGLPLVDLVSFSDSKLASELSSNCGEEGPTSNIEVLEKRLKQIRNFIFVLFVSLGVPVLNMGDECGQSSGGSVASNDRRSFNWDLLKTDFGTQTTQFIAFFSSFRSRRFDLFQSRNFLKGENIDWFDNNQSSPQWEDPSCKFLAVMLRADKEENGSITENAKPRSDIFMAFNASDQSESVALPEPSEGTSWFRLVDTALSFPGFFSFNGELVPTTGSVTYEMQAHSCALFEAKSSND
Homology
BLAST of Cla97C09G169430 vs. NCBI nr
Match: XP_038898155.1 (isoamylase 2, chloroplastic [Benincasa hispida])

HSP 1 Score: 1634.8 bits (4232), Expect = 0.0e+00
Identity = 806/885 (91.07%), Postives = 834/885 (94.24%), Query Frame = 0

Query: 3   MATFPSSLVIQPFCVYNCGVTESFKLAVSDHHTYGQKIKYQSGKMDEARMLAHGERKVSF 62
           MATF SSLVIQPFCVYN GVTESFKLAV DHHTYGQKIKY SGKMDEARMLAHGE +V  
Sbjct: 1   MATFLSSLVIQPFCVYNSGVTESFKLAVGDHHTYGQKIKYHSGKMDEARMLAHGESRVGS 60

Query: 63  MKSSHRNLSIAYATSGISVGKSGQRFSTGGKSKEHRKVATYLFRTEFGDLVNVFVEKTGS 122
            KSSHRNLS  YATSGI VGKSGQRF TGGKSKEH KVATYLFRTEFGDLVNVFV KTGS
Sbjct: 61  TKSSHRNLSKVYATSGILVGKSGQRFGTGGKSKEHVKVATYLFRTEFGDLVNVFVGKTGS 120

Query: 123 GFTVNIEISSVQRANIDETLLLSWGVYRSDSTLVTPNFQSSLPDETIGATETPFLKTSEG 182
           GF VN+EISSVQ A+I++TLLLSWGVYRSDS +VTPNFQSSLPDETIGATETPF+KTS G
Sbjct: 121 GFMVNVEISSVQLASIEKTLLLSWGVYRSDSAIVTPNFQSSLPDETIGATETPFVKTSGG 180

Query: 183 KFAVELEFDAKHTPFYLSFLLKYSLGVDSGSSEIRSHRKTSFPVPVGFGRGYPSPLGLSI 242
           KFAVELEFDAKHTPFYLSFLLKY +GVDSGSSEIRSHRKTSF VPVGFGRGYPSPLGLSI
Sbjct: 181 KFAVELEFDAKHTPFYLSFLLKYPVGVDSGSSEIRSHRKTSFTVPVGFGRGYPSPLGLSI 240

Query: 243 SGDGSINFSIFSSTAESVVLCLYNDSTSEKPVLELDLDPYINKSGNIWHASFEGASKFVS 302
           SGDGSINFSIFSSTAESVVLCLYNDSTSEKPVLELDLD YIN+SG+IWHASFE AS FVS
Sbjct: 241 SGDGSINFSIFSSTAESVVLCLYNDSTSEKPVLELDLDSYINRSGSIWHASFESASNFVS 300

Query: 303 YGYRCKGSKFHENQDGVEVSQIVVDPYAKILTASIPKARGQGLGLPSNLLGQICKVPAFD 362
           YGYRCKGSKFHENQDGVEV++IVVDPYAKILTAS+ K  GQGLGLP   LGQICKVPAFD
Sbjct: 301 YGYRCKGSKFHENQDGVEVNRIVVDPYAKILTASVAKTLGQGLGLP-KFLGQICKVPAFD 360

Query: 363 WDGEVRPNLPMEKLFVYRLNVKHFTIDKSSQLPADIAGTFSGLTKKLLHFKNLGVNAVQL 422
           WDGEV PNLPMEKLF YRLNVK FT+DKSSQLPADIAGTFSGLTKKLLHFKNLGVNAV L
Sbjct: 361 WDGEVCPNLPMEKLFAYRLNVKRFTVDKSSQLPADIAGTFSGLTKKLLHFKNLGVNAVLL 420

Query: 423 EPIFQFDEKEGPYFPFHFFSPTNSYGPSGASISVINSMKEMVKELHANGIEVILEVVFTH 482
           EPIFQFDEKEG YFPFHFFSP N+YGPSG SIS INSMK MVK+LHANG+EVILEVVFTH
Sbjct: 421 EPIFQFDEKEGLYFPFHFFSPANNYGPSGVSISAINSMKGMVKQLHANGVEVILEVVFTH 480

Query: 483 TAVDGALQGIDDSSYYFVNRVANLEGKSVLNCNYPIVQQLLLDSLRYWVTEFHIDGFCFI 542
           TA+DGALQGIDDSSYYFVNRVANLEGKS LNCNYPIVQQLLLDSLRYWVTEFHIDGFCFI
Sbjct: 481 TAMDGALQGIDDSSYYFVNRVANLEGKSALNCNYPIVQQLLLDSLRYWVTEFHIDGFCFI 540

Query: 543 NASSLLRGHHGELLSRPPFVEAVAFDPLLSKTKLVADFWDPQDLVSKETHFPHWKRWAEV 602
           NASSLLRGHHGE LSR PFVEA+AFDPLLSKTKLVADFWDPQ+LVS+ET FPHWKRWAEV
Sbjct: 541 NASSLLRGHHGEFLSRSPFVEAIAFDPLLSKTKLVADFWDPQELVSEETSFPHWKRWAEV 600

Query: 603 NSKFCNDIRDFFRGEGLISSLATRLCGSGDVFSDGRGPAFSFNFIARNVGLPLVDLVSFS 662
           NSKFCND+RDFFRGEGLISSLATRLCGSGDVFS+GRGPAFSFNFI RNVGLPLVDLVSFS
Sbjct: 601 NSKFCNDVRDFFRGEGLISSLATRLCGSGDVFSNGRGPAFSFNFITRNVGLPLVDLVSFS 660

Query: 663 DSKLASELSSNCGEEGPTSNIEVLEKRLKQIRNFIFVLFVSLGVPVLNMGDECGQSSGGS 722
           +SKLASELS NCGEEGPTSNIEVLEKRLKQIRNFIFV+FVSLGVPVLNMGDECGQS+GGS
Sbjct: 661 NSKLASELSWNCGEEGPTSNIEVLEKRLKQIRNFIFVVFVSLGVPVLNMGDECGQSAGGS 720

Query: 723 VASNDRRSFNWDLLKTDFGTQTTQFIAFFSSFRSRRFDLFQSRNFLKGENIDWFDNNQSS 782
           VA NDRRSFNWDLLKTDFGTQTTQFI F SSFRSRRFDLFQSRNFLKGENIDWFDNNQS 
Sbjct: 721 VAFNDRRSFNWDLLKTDFGTQTTQFITFLSSFRSRRFDLFQSRNFLKGENIDWFDNNQSP 780

Query: 783 PQWEDPSCKFLAVMLRADKEENGSITENAKPRSDIFMAFNASDQSESVALPEPSEGTSWF 842
           PQWEDPSCKFLAVMLRADKEENGSITENAKPRSDIFMAFNASDQSE+VALPEPSEGTSWF
Sbjct: 781 PQWEDPSCKFLAVMLRADKEENGSITENAKPRSDIFMAFNASDQSETVALPEPSEGTSWF 840

Query: 843 RLVDTALSFPGFFSFNGELVPTTGSVTYEMQAHSCALFEAKSSND 888
           RLVDTALSFPGFFS +GE VPTTGSVTYEMQAHSCALFEAKS ND
Sbjct: 841 RLVDTALSFPGFFSSDGEQVPTTGSVTYEMQAHSCALFEAKSFND 884

BLAST of Cla97C09G169430 vs. NCBI nr
Match: XP_008451396.1 (PREDICTED: isoamylase 2, chloroplastic [Cucumis melo])

HSP 1 Score: 1597.0 bits (4134), Expect = 0.0e+00
Identity = 787/884 (89.03%), Postives = 822/884 (92.99%), Query Frame = 0

Query: 3   MATFPSSLVIQPFCVYNCGVTESFKLAVSDHHTYGQKIKYQSGKMDEARMLAHGERKVSF 62
           MATF SSLVIQPFCV+NCGVTES KLAVSDH TYGQK KYQSGKMDEARMLAHGE KV  
Sbjct: 1   MATFLSSLVIQPFCVHNCGVTESLKLAVSDHLTYGQKTKYQSGKMDEARMLAHGESKVGA 60

Query: 63  MKSSHRNLSIAYATSGISVGKSGQRFSTGGKSKEHRKVATYLFRTEFGDLVNVFVEKTGS 122
           +KSSHRNLS AY TSGI VGKSGQR   GGKSKE RKVATYLFRTEFGDLVNVFV KTGS
Sbjct: 61  VKSSHRNLSKAYVTSGILVGKSGQRLGIGGKSKEQRKVATYLFRTEFGDLVNVFVGKTGS 120

Query: 123 GFTVNIEISSVQRANIDETLLLSWGVYRSDSTLVTPNFQSSLPDETIGATETPFLKTSEG 182
            FTVNIEISS+Q  + DETLLLSW VYRSDS  VT NFQSS PD T GATETPF+KTSEG
Sbjct: 121 AFTVNIEISSMQLVSTDETLLLSWRVYRSDSAPVTSNFQSSPPDGTTGATETPFVKTSEG 180

Query: 183 KFAVELEFDAKHTPFYLSFLLKYSLGVDSGSSEIRSHRKTSFPVPVGFGRGYPSPLGLSI 242
           KF+VELEFDAKHTPFYLSF+LK+ +GVDSGSSEIRSHRKTSF VPVGFGRGYPSPLGLSI
Sbjct: 181 KFSVELEFDAKHTPFYLSFVLKFPMGVDSGSSEIRSHRKTSFSVPVGFGRGYPSPLGLSI 240

Query: 243 SGDGSINFSIFSSTAESVVLCLYNDSTSEKPVLELDLDPYINKSGNIWHASFEGASKFVS 302
           SGDGSINFSIFSSTAESVVLCLYNDSTSEKP+LELDLDPYIN+SGNIWHASFEGAS FVS
Sbjct: 241 SGDGSINFSIFSSTAESVVLCLYNDSTSEKPLLELDLDPYINRSGNIWHASFEGASNFVS 300

Query: 303 YGYRCKGSKFHENQDGVEVSQIVVDPYAKILTASIPKARGQGLGLPSNLLGQICKVPAFD 362
           YGY+CKG K HENQDG+EVS+IVVDPYAKIL  SIPK+R QGLG P   LGQI  VPAFD
Sbjct: 301 YGYQCKGLKSHENQDGLEVSRIVVDPYAKILAPSIPKSRDQGLGFPLKFLGQISIVPAFD 360

Query: 363 WDGEVRPNLPMEKLFVYRLNVKHFTIDKSSQLPADIAGTFSGLTKKLLHFKNLGVNAVQL 422
           WDGEVRPNLPMEKLFVYRLNVK FTIDKSSQLPADIAGTFSGLTKKLLH KNLGVNAV L
Sbjct: 361 WDGEVRPNLPMEKLFVYRLNVKRFTIDKSSQLPADIAGTFSGLTKKLLHLKNLGVNAVLL 420

Query: 423 EPIFQFDEKEGPYFPFHFFSPTNSYGPSGASISVINSMKEMVKELHANGIEVILEVVFTH 482
           EPIFQFDEKEGPYFPFHFFSPTN+YGPSGASIS INSMKEMVKELHANG+EVI+EVV+TH
Sbjct: 421 EPIFQFDEKEGPYFPFHFFSPTNNYGPSGASISAINSMKEMVKELHANGVEVIVEVVYTH 480

Query: 483 TAVDGALQGIDDSSYYFVNRVANLEGKSVLNCNYPIVQQLLLDSLRYWVTEFHIDGFCFI 542
           T+VDGALQGIDDSSYYF NRVANLE +S LNCNYPIVQQLLLDSLRYWVTEFH+DGFCF+
Sbjct: 481 TSVDGALQGIDDSSYYFANRVANLEERSALNCNYPIVQQLLLDSLRYWVTEFHVDGFCFV 540

Query: 543 NASSLLRGHHGELLSRPPFVEAVAFDPLLSKTKLVADFWDPQDLVSKETHFPHWKRWAEV 602
           NAS LL+GHHGELLSRPPFVEA+AFDP+LSKTKLVADFWDPQ+LVSKET FPHWKRWAEV
Sbjct: 541 NASFLLQGHHGELLSRPPFVEAIAFDPILSKTKLVADFWDPQELVSKETRFPHWKRWAEV 600

Query: 603 NSKFCNDIRDFFRGEGLISSLATRLCGSGDVFSDGRGPAFSFNFIARNVGLPLVDLVSFS 662
           NSKFC+DIRDFFRGEGLISSLATRLCGSGDVFSDGRGPAFSFNFIARNVGLPLVDLVSFS
Sbjct: 601 NSKFCSDIRDFFRGEGLISSLATRLCGSGDVFSDGRGPAFSFNFIARNVGLPLVDLVSFS 660

Query: 663 DSKLASELSSNCGEEGPTSNIEVLEKRLKQIRNFIFVLFVSLGVPVLNMGDECGQSSGGS 722
           +S LASELS NCGEEGPT+ ++VLEKRLKQIRNFIFVLFVSLGVPVLNMGDECGQSSGGS
Sbjct: 661 NSNLASELSWNCGEEGPTNTLKVLEKRLKQIRNFIFVLFVSLGVPVLNMGDECGQSSGGS 720

Query: 723 VASNDRRSFNWDLLKTDFGTQTTQFIAFFSSFRSRRFDLFQSRNFLKGENIDWFDNNQSS 782
           VA NDRRSFNWDLLKTDFGTQTTQFIAF SSFRSRRFDLFQSRNFLKGENIDWFDNNQS 
Sbjct: 721 VAFNDRRSFNWDLLKTDFGTQTTQFIAFLSSFRSRRFDLFQSRNFLKGENIDWFDNNQSP 780

Query: 783 PQWEDPSCKFLAVMLRADKEENGSITENAKPRSDIFMAFNASDQSESVALPEPSEGTSWF 842
           PQWED SCKFLAVMLRADKEEN SITEN K RSDIFMAFNASDQSESVALPEPSEGTSWF
Sbjct: 781 PQWEDASCKFLAVMLRADKEENESITENPKTRSDIFMAFNASDQSESVALPEPSEGTSWF 840

Query: 843 RLVDTALSFPGFFSFNGELVPTTGSVTYEMQAHSCALFEAKSSN 887
           R+VDTAL FPGFFS +GELVP TGSVTYEMQAHSCALFEAKS+N
Sbjct: 841 RVVDTALPFPGFFSSDGELVPMTGSVTYEMQAHSCALFEAKSAN 884

BLAST of Cla97C09G169430 vs. NCBI nr
Match: XP_004136188.1 (isoamylase 2, chloroplastic [Cucumis sativus] >KGN44865.1 hypothetical protein Csa_016566 [Cucumis sativus])

HSP 1 Score: 1596.6 bits (4133), Expect = 0.0e+00
Identity = 782/885 (88.36%), Postives = 824/885 (93.11%), Query Frame = 0

Query: 3   MATFPSSLVIQPFCVYNCGVTESFKLAVSDHHTYGQKIKYQSGKMDEARMLAHGERKVSF 62
           MATF SSLVIQPFCVYNCGVTES KLA SDH TYGQK KYQ GKMDEARMLAHGE KV  
Sbjct: 1   MATFLSSLVIQPFCVYNCGVTESPKLAASDHLTYGQKTKYQFGKMDEARMLAHGENKVGA 60

Query: 63  MKSSHRNLSIAYATSGISVGKSGQRFSTGGKSKEHRKVATYLFRTEFGDLVNVFVEKTGS 122
           +KSSHRNLS  YA SGISVGKSGQR   GGKSKE R+VATYLFRTEFGDLVNVFV K GS
Sbjct: 61  VKSSHRNLSKTYAKSGISVGKSGQRLGIGGKSKEQRRVATYLFRTEFGDLVNVFVGKKGS 120

Query: 123 GFTVNIEISSVQRANIDETLLLSWGVYRSDSTLVTPNFQSSLPDETIGATETPFLKTSEG 182
            FTVNIE+ S+Q  +IDE LLLSWGVYRSDS LVTPNF+SS PDET GATETPF+KTSEG
Sbjct: 121 TFTVNIEVPSMQLVSIDEALLLSWGVYRSDSALVTPNFESSPPDETTGATETPFVKTSEG 180

Query: 183 KFAVELEFDAKHTPFYLSFLLKYSLGVDSGSSEIRSHRKTSFPVPVGFGRGYPSPLGLSI 242
           KF+VELEFDAKHTPFYLSF+LKY +GVDSGSSEIRSH+KTSF VPVGFGRGYPSPLGLSI
Sbjct: 181 KFSVELEFDAKHTPFYLSFVLKYPMGVDSGSSEIRSHKKTSFSVPVGFGRGYPSPLGLSI 240

Query: 243 SGDGSINFSIFSSTAESVVLCLYNDSTSEKPVLELDLDPYINKSGNIWHASFEGASKFVS 302
           SGDGS+NFSIFSS+AES+VLCLYNDSTSEKP+LELDLDPYIN+SGNIWHASFEGASKFVS
Sbjct: 241 SGDGSVNFSIFSSSAESLVLCLYNDSTSEKPLLELDLDPYINRSGNIWHASFEGASKFVS 300

Query: 303 YGYRCKGSKFHENQDGVEVSQIVVDPYAKILTASIPKARGQGLGLPSNLLGQICKVPAFD 362
           YGY+CKGSK HENQDG+EVS+IVVDPYAKIL  SIPK+ GQGLGLPS  LGQI KVP FD
Sbjct: 301 YGYQCKGSKSHENQDGLEVSRIVVDPYAKILAPSIPKSSGQGLGLPSKFLGQISKVPTFD 360

Query: 363 WDGEVRPNLPMEKLFVYRLNVKHFTIDKSSQLPADIAGTFSGLTKKLLHFKNLGVNAVQL 422
           WDGEV PNLPMEKLFVYRLNV+ FT+DKSSQLPADIAGTFSGLTKKLLHFKNLGVNAV L
Sbjct: 361 WDGEVHPNLPMEKLFVYRLNVERFTMDKSSQLPADIAGTFSGLTKKLLHFKNLGVNAVLL 420

Query: 423 EPIFQFDEKEGPYFPFHFFSPTNSYGPSGASISVINSMKEMVKELHANGIEVILEVVFTH 482
           EPIFQFDEKEGPYFPFHFFSPTN+YGPSGASIS INSMKEMVKELHANG+EVILEVV+TH
Sbjct: 421 EPIFQFDEKEGPYFPFHFFSPTNNYGPSGASISAINSMKEMVKELHANGVEVILEVVYTH 480

Query: 483 TAVDGALQGIDDSSYYFVNRVANLEGKSVLNCNYPIVQQLLLDSLRYWVTEFHIDGFCFI 542
           T+ +GALQGIDDSSYYF NRVANLE KS LNCNYPIVQQLLLDSLRYWVTEFH+DGFCF+
Sbjct: 481 TSGNGALQGIDDSSYYFTNRVANLEEKSALNCNYPIVQQLLLDSLRYWVTEFHVDGFCFV 540

Query: 543 NASSLLRGHHGELLSRPPFVEAVAFDPLLSKTKLVADFWDPQDLVSKETHFPHWKRWAEV 602
           NAS LLRGHHGELLSRPPFVEA+AFDPLLSKTKLVADFWDPQ+L SKET FPHWKRWAEV
Sbjct: 541 NASFLLRGHHGELLSRPPFVEAIAFDPLLSKTKLVADFWDPQELESKETRFPHWKRWAEV 600

Query: 603 NSKFCNDIRDFFRGEGLISSLATRLCGSGDVFSDGRGPAFSFNFIARNVGLPLVDLVSFS 662
           NSKFC+DIRDFFRGEGLISSLATRLCGSGDVFSDGRGPAFSFNFIARNVGLPLVDLVSFS
Sbjct: 601 NSKFCSDIRDFFRGEGLISSLATRLCGSGDVFSDGRGPAFSFNFIARNVGLPLVDLVSFS 660

Query: 663 DSKLASELSSNCGEEGPTSNIEVLEKRLKQIRNFIFVLFVSLGVPVLNMGDECGQSSGGS 722
           +S LASELS NCGEEGPTSN++VLEKRLKQIRNFIFVLFVSLGVPVLNMGDECGQSSGGS
Sbjct: 661 NSNLASELSWNCGEEGPTSNLKVLEKRLKQIRNFIFVLFVSLGVPVLNMGDECGQSSGGS 720

Query: 723 VASNDRRSFNWDLLKTDFGTQTTQFIAFFSSFRSRRFDLFQSRNFLKGENIDWFDNNQSS 782
           VA ND+RSFNWDLLKTDFGTQTTQFIAF SSFRSRRFDLFQ+RNFLKGENIDWFDNNQS 
Sbjct: 721 VAFNDKRSFNWDLLKTDFGTQTTQFIAFLSSFRSRRFDLFQNRNFLKGENIDWFDNNQSP 780

Query: 783 PQWEDPSCKFLAVMLRADKEENGSITENAKPRSDIFMAFNASDQSESVALPEPSEGTSWF 842
           PQWED SCKFLAVMLRADKEEN SITEN K RS+IFM FNASDQSESVALPEP EGTSWF
Sbjct: 781 PQWEDASCKFLAVMLRADKEENESITENPKTRSNIFMVFNASDQSESVALPEPLEGTSWF 840

Query: 843 RLVDTALSFPGFFSFNGELVPTTGSVTYEMQAHSCALFEAKSSND 888
           R+VDTAL FPGFFS +GELVP TGSVTYE+QAHSCALFEAKS+ND
Sbjct: 841 RVVDTALPFPGFFSSDGELVPMTGSVTYEIQAHSCALFEAKSAND 885

BLAST of Cla97C09G169430 vs. NCBI nr
Match: KAA0057598.1 (isoamylase 2 [Cucumis melo var. makuwa] >TYK20996.1 isoamylase 2 [Cucumis melo var. makuwa])

HSP 1 Score: 1595.5 bits (4130), Expect = 0.0e+00
Identity = 786/884 (88.91%), Postives = 821/884 (92.87%), Query Frame = 0

Query: 3   MATFPSSLVIQPFCVYNCGVTESFKLAVSDHHTYGQKIKYQSGKMDEARMLAHGERKVSF 62
           MATF SSLVIQPFCV+NCGVTES KLAVSDH TYGQK KYQSGKMDEARMLAHGE KV  
Sbjct: 1   MATFLSSLVIQPFCVHNCGVTESLKLAVSDHLTYGQKTKYQSGKMDEARMLAHGESKVGA 60

Query: 63  MKSSHRNLSIAYATSGISVGKSGQRFSTGGKSKEHRKVATYLFRTEFGDLVNVFVEKTGS 122
           +KSSHRNLS AY TSGISVGKSGQR   GGKSKE RKVATYLFRTEFGDLVNVFV KTGS
Sbjct: 61  VKSSHRNLSKAYVTSGISVGKSGQRLGIGGKSKEQRKVATYLFRTEFGDLVNVFVGKTGS 120

Query: 123 GFTVNIEISSVQRANIDETLLLSWGVYRSDSTLVTPNFQSSLPDETIGATETPFLKTSEG 182
            FTVNIEISS+Q  + DETLLLSW VYRSDS  VT NFQSS PD T GATETPF+KTSEG
Sbjct: 121 AFTVNIEISSMQLVSTDETLLLSWRVYRSDSAPVTSNFQSSPPDGTTGATETPFVKTSEG 180

Query: 183 KFAVELEFDAKHTPFYLSFLLKYSLGVDSGSSEIRSHRKTSFPVPVGFGRGYPSPLGLSI 242
           KF+VELEFDAKHTPFYLSF+LK+ +GVDSGSSEIRSHRKTSF VPVGFGRGYPSP GLSI
Sbjct: 181 KFSVELEFDAKHTPFYLSFVLKFPMGVDSGSSEIRSHRKTSFSVPVGFGRGYPSPSGLSI 240

Query: 243 SGDGSINFSIFSSTAESVVLCLYNDSTSEKPVLELDLDPYINKSGNIWHASFEGASKFVS 302
           SGDGSINFSIFSSTAESVVLCLYNDSTSEKP+LELDLDPYIN+SGNIWHASFEG S FVS
Sbjct: 241 SGDGSINFSIFSSTAESVVLCLYNDSTSEKPLLELDLDPYINRSGNIWHASFEGVSNFVS 300

Query: 303 YGYRCKGSKFHENQDGVEVSQIVVDPYAKILTASIPKARGQGLGLPSNLLGQICKVPAFD 362
           YGY+CKG K HENQDG+EVS+IVVDPYAKIL  SIPK+R QGLG P   LGQI  VPAFD
Sbjct: 301 YGYQCKGLKSHENQDGLEVSRIVVDPYAKILAPSIPKSRDQGLGFPLKFLGQISIVPAFD 360

Query: 363 WDGEVRPNLPMEKLFVYRLNVKHFTIDKSSQLPADIAGTFSGLTKKLLHFKNLGVNAVQL 422
           WDGEVRPNLPMEKLFVYRLNVK FTIDKSSQLPADIAGTFSGLTKKLLH KNLGVNAV L
Sbjct: 361 WDGEVRPNLPMEKLFVYRLNVKRFTIDKSSQLPADIAGTFSGLTKKLLHLKNLGVNAVLL 420

Query: 423 EPIFQFDEKEGPYFPFHFFSPTNSYGPSGASISVINSMKEMVKELHANGIEVILEVVFTH 482
           EPIFQFDEKEGPYFPFHFFSPTN+YGPSGASIS INSMKEMVKELHANG+EVI+EVV+TH
Sbjct: 421 EPIFQFDEKEGPYFPFHFFSPTNNYGPSGASISAINSMKEMVKELHANGVEVIVEVVYTH 480

Query: 483 TAVDGALQGIDDSSYYFVNRVANLEGKSVLNCNYPIVQQLLLDSLRYWVTEFHIDGFCFI 542
           T+VDGALQGIDDSSYYF NRVANLE +S LNCNYPIVQQLLLDSLRYWVTEFH+DGFCF+
Sbjct: 481 TSVDGALQGIDDSSYYFANRVANLEERSALNCNYPIVQQLLLDSLRYWVTEFHVDGFCFV 540

Query: 543 NASSLLRGHHGELLSRPPFVEAVAFDPLLSKTKLVADFWDPQDLVSKETHFPHWKRWAEV 602
           NAS LL+GHHGELLSRPPFVEA+AFDP+LSKTKLVADFWDPQ+LVSKET FPHWKRWAEV
Sbjct: 541 NASFLLQGHHGELLSRPPFVEAIAFDPILSKTKLVADFWDPQELVSKETRFPHWKRWAEV 600

Query: 603 NSKFCNDIRDFFRGEGLISSLATRLCGSGDVFSDGRGPAFSFNFIARNVGLPLVDLVSFS 662
           NSKFC+DIRDFFRGEGLISSLATRLCGSGDVFSDGRGPAFSFNFIARNVGLPLVDLVSFS
Sbjct: 601 NSKFCSDIRDFFRGEGLISSLATRLCGSGDVFSDGRGPAFSFNFIARNVGLPLVDLVSFS 660

Query: 663 DSKLASELSSNCGEEGPTSNIEVLEKRLKQIRNFIFVLFVSLGVPVLNMGDECGQSSGGS 722
           +S LASELS NCGEEGPT+ ++VLEKRLKQIRNFIFVLFVSLGVPVLNMGDECGQSSGGS
Sbjct: 661 NSNLASELSWNCGEEGPTNTLKVLEKRLKQIRNFIFVLFVSLGVPVLNMGDECGQSSGGS 720

Query: 723 VASNDRRSFNWDLLKTDFGTQTTQFIAFFSSFRSRRFDLFQSRNFLKGENIDWFDNNQSS 782
           VA NDRRSFNWDLLKTDFGTQTTQFIAF SSFRSRRFDLFQSRNFLKGENIDWFDNNQS 
Sbjct: 721 VAFNDRRSFNWDLLKTDFGTQTTQFIAFLSSFRSRRFDLFQSRNFLKGENIDWFDNNQSP 780

Query: 783 PQWEDPSCKFLAVMLRADKEENGSITENAKPRSDIFMAFNASDQSESVALPEPSEGTSWF 842
           PQWED SCKFLAVMLRADKEEN SITEN K RSDIFMAFNASDQSESVALPEPSEGTSWF
Sbjct: 781 PQWEDASCKFLAVMLRADKEENESITENPKTRSDIFMAFNASDQSESVALPEPSEGTSWF 840

Query: 843 RLVDTALSFPGFFSFNGELVPTTGSVTYEMQAHSCALFEAKSSN 887
           R+VDTAL FPGFFS +GELVP TGSVTYEMQAHSCALFEAKS+N
Sbjct: 841 RVVDTALPFPGFFSSDGELVPMTGSVTYEMQAHSCALFEAKSAN 884

BLAST of Cla97C09G169430 vs. NCBI nr
Match: KAG7014422.1 (Isoamylase 2, chloroplastic [Cucurbita argyrosperma subsp. argyrosperma])

HSP 1 Score: 1573.1 bits (4072), Expect = 0.0e+00
Identity = 769/885 (86.89%), Postives = 821/885 (92.77%), Query Frame = 0

Query: 3   MATFPSSLVIQPFCVYNCGVTESFKLAVSDHHTYGQKIKYQSGKMDEARMLAHGERKVSF 62
           MATF SSL IQPFC YN G TESFKLAV+D+HTYGQK+ YQSGK+DEARM AHGE KV F
Sbjct: 24  MATFLSSL-IQPFCGYNRGGTESFKLAVNDYHTYGQKMNYQSGKLDEARMQAHGESKVGF 83

Query: 63  MKSSHRNLSIAYATSGISVGKSGQRFSTGGKSKEHRKVATYLFRTEFGDLVNVFVEKTGS 122
            KS HRN+  AYA SGISVGKSGQ+F TGGK++E RKVATYLFRTEFGDLV  FV K GS
Sbjct: 84  TKSLHRNVCKAYAPSGISVGKSGQKFGTGGKTEEDRKVATYLFRTEFGDLVYAFVGKRGS 143

Query: 123 GFTVNIEISSVQRANIDETLLLSWGVYRSDSTLVTPNFQSSLPDETIGATETPFLKTSEG 182
           GF V+IEISS++ A+IDETLLLSWGVYRS+S +VTPNFQS LP+ETIGATETPF++TSEG
Sbjct: 144 GFLVSIEISSLKLASIDETLLLSWGVYRSNSAIVTPNFQSILPEETIGATETPFVRTSEG 203

Query: 183 KFAVELEFDAKHTPFYLSFLLKYSLGVDSGSSEIRSHRKTSFPVPVGFGRGYPSPLGLSI 242
           KFAVELEFDAKH PFYLSFLLKY +GVDSGSSEI+SHRKTSF VPVGFGRGYPSPLGLSI
Sbjct: 204 KFAVELEFDAKHDPFYLSFLLKYPVGVDSGSSEIKSHRKTSFSVPVGFGRGYPSPLGLSI 263

Query: 243 SGDGSINFSIFSSTAESVVLCLYNDSTSEKPVLELDLDPYINKSGNIWHASFEGASKFVS 302
           SGDGSINFSIFSSTAESVVLCLYNDSTSEKPVLELDLDPYIN+SGNIWH SFEGAS FVS
Sbjct: 264 SGDGSINFSIFSSTAESVVLCLYNDSTSEKPVLELDLDPYINRSGNIWHVSFEGASNFVS 323

Query: 303 YGYRCKGSKFHENQDGVEVSQIVVDPYAKILTASIPKARGQGLGLPSNLLGQICKVPAFD 362
           YGYRC+GSKFHENQD  EVS+I+VDPYAKILTAS PK RGQG+GLPS  LGQIC VPAF 
Sbjct: 324 YGYRCRGSKFHENQDEAEVSRILVDPYAKILTASTPKTRGQGVGLPSKFLGQICNVPAFS 383

Query: 363 WDGEVRPNLPMEKLFVYRLNVKHFTIDKSSQLPADIAGTFSGLTKKLLHFKNLGVNAVQL 422
           WDGEVRPNLPMEKLF+YRLNVKHFT+DKSSQLP DIAGTFSGLT+KLLHFK LGVNAV L
Sbjct: 384 WDGEVRPNLPMEKLFIYRLNVKHFTVDKSSQLPIDIAGTFSGLTQKLLHFKKLGVNAVLL 443

Query: 423 EPIFQFDEKEGPYFPFHFFSPTNSYGPSGASISVINSMKEMVKELHANGIEVILEVVFTH 482
           EPIF FDEK+GPYFPFHFFSPTN Y PSGASIS +NS+KEMVKELHANG+EVILEVVFTH
Sbjct: 444 EPIFPFDEKDGPYFPFHFFSPTNIYVPSGASISSLNSVKEMVKELHANGVEVILEVVFTH 503

Query: 483 TAVDGALQGIDDSSYYFVNRVANLEGKSVLNCNYPIVQQLLLDSLRYWVTEFHIDGFCFI 542
           TA+DGALQGIDDS YYFVNRVAN EGKS LNCN+PIVQQLLLDSLRYWVTEFHIDGFCFI
Sbjct: 504 TAMDGALQGIDDSCYYFVNRVANSEGKSALNCNHPIVQQLLLDSLRYWVTEFHIDGFCFI 563

Query: 543 NASSLLRGHHGELLSRPPFVEAVAFDPLLSKTKLVADFWDPQDLVSKETHFPHWKRWAEV 602
           NASSLL+GHHGELLSRPPFVEA+AFDPLLSKTK++A+FWDPQ+LV KET FPHWKRWAE+
Sbjct: 564 NASSLLQGHHGELLSRPPFVEAIAFDPLLSKTKIIAEFWDPQELVPKETRFPHWKRWAEL 623

Query: 603 NSKFCNDIRDFFRGEGLISSLATRLCGSGDVFSDGRGPAFSFNFIARNVGLPLVDLVSFS 662
           NSKF ND+++FFRGEGL+SSLATRLCGSGDVFSDGRGPAFSFNFIARNVGLPLVDLVSFS
Sbjct: 624 NSKFSNDMKEFFRGEGLVSSLATRLCGSGDVFSDGRGPAFSFNFIARNVGLPLVDLVSFS 683

Query: 663 DSKLASELSSNCGEEGPTSNIEVLEKRLKQIRNFIFVLFVSLGVPVLNMGDECGQSSGGS 722
            SKLASELS NCGEEGPTSNIEVLEKRLKQIRNFIFVLFVSLGVPVLNMGDECGQSSGGS
Sbjct: 684 SSKLASELSWNCGEEGPTSNIEVLEKRLKQIRNFIFVLFVSLGVPVLNMGDECGQSSGGS 743

Query: 723 VASNDRRSFNWDLLKTDFGTQTTQFIAFFSSFRSRRFDLFQSRNFLKGENIDWFDNNQSS 782
           VA  DR+SFNWD LKTDFGTQTT+FIAFFSSFRSRRFDLFQSR+FLKGENIDWFDNNQS 
Sbjct: 744 VAFKDRKSFNWDSLKTDFGTQTTRFIAFFSSFRSRRFDLFQSRHFLKGENIDWFDNNQSP 803

Query: 783 PQWEDPSCKFLAVMLRADKEENGSITENAKPRSDIFMAFNASDQSESVALPEPSEGTSWF 842
           P+WEDPSCKFLAVMLRADKEE+GSITEN KP+SDIFMAFNASDQS SVALPEPSEGT+WF
Sbjct: 804 PEWEDPSCKFLAVMLRADKEESGSITENPKPQSDIFMAFNASDQSVSVALPEPSEGTNWF 863

Query: 843 RLVDTALSFPGFFSFNGELVPTTGSVTYEMQAHSCALFEAKSSND 888
           RLVDTALSFPGFFS +GE VPTT  VTYEMQAHSC LFEAKS ND
Sbjct: 864 RLVDTALSFPGFFSSDGEPVPTTSPVTYEMQAHSCTLFEAKSFND 907

BLAST of Cla97C09G169430 vs. ExPASy Swiss-Prot
Match: Q8L735 (Isoamylase 2, chloroplastic OS=Arabidopsis thaliana OX=3702 GN=ISA2 PE=1 SV=2)

HSP 1 Score: 933.3 bits (2411), Expect = 2.0e-270
Identity = 477/890 (53.60%), Postives = 628/890 (70.56%), Query Frame = 0

Query: 3   MATFPSSLVIQPFCVYNCGVTESFKLAVSDHHT-YGQKIKYQSGKMDEARMLAHGERKVS 62
           MA +  S+ I   C+ N G+T ++K   +   T    KIK  S  +   R    G+   S
Sbjct: 1   MAAWSPSVGIGSCCLNN-GITRTWKFPSARLFTGRKNKIKLGSETLMFTRKRFMGDLVTS 60

Query: 63  FMKSSHRNLSIAYATSGISVGKSGQRFSTGGKSKEHRKVATYLFRTEFGDLVNVFVEKTG 122
            ++S   +   A  T   S+       S   ++ + +KV +Y FRT+ G LV V VEK  
Sbjct: 61  ALQSYQFSKICASKT---SIELREALSSRRAEADDLKKVTSYSFRTKAGALVKVKVEKKR 120

Query: 123 SGFTVNIEISSVQRANIDET-LLLSWGVYRSDSTLVTP-NFQSSLPDETIGATETPFLKT 182
             +++ + +SS++ +  D++ L++ WGVYRSDS+   P +F++S  D     TET F+K+
Sbjct: 121 EKYSILVYVSSLELSGDDKSRLVMVWGVYRSDSSCFLPLDFENSSQDSQTHTTETTFVKS 180

Query: 183 SEGKFAVELEFDAKHTPFYLSFLLKYSLGVDSGSSEIRSHRKTSFPVPVGFGRGYPSPLG 242
           S  +  + LEFD K +PFYLSF LK   G D    E+ +HR T F +PVGF  G+P PLG
Sbjct: 181 SLSELMLGLEFDGKESPFYLSFHLKLVSGRDPDGQEMLTHRDTDFCIPVGFTAGHPLPLG 240

Query: 243 LSIS-GDGSINFSIFSSTAESVVLCLYNDSTSEKPVLELDLDPYINKSGNIWHASFEGAS 302
           LS    D S NFS FS ++ +VVLCLY+DST++KP LELDLDPY+N++G++WHAS +   
Sbjct: 241 LSSGPDDDSWNFSFFSRSSTNVVLCLYDDSTTDKPALELDLDPYVNRTGDVWHASVDNTW 300

Query: 303 KFVSYGYRCKGSKFHENQDGVEVSQIVVDPYAKILTASIPKARGQGLGLPSNLLGQICKV 362
            FV YGYRCK +   +    VE   IV+DPYA ++          G  +    LG + K 
Sbjct: 301 DFVRYGYRCKETAHSKEDVDVEGEPIVLDPYATVV----------GKSVSQKYLGSLSKS 360

Query: 363 PAFDWDGEVRPNLPMEKLFVYRLNVKHFTIDKSSQLPADIAGTFSGLTKKLLHFKNLGVN 422
           P+FDW  +V PN+P+EKL VYRLNVK FT  +SS+LP+++AGTFSG+ +K+ H K LG N
Sbjct: 361 PSFDWGEDVSPNIPLEKLLVYRLNVKGFTQHRSSKLPSNVAGTFSGVAEKVSHLKTLGTN 420

Query: 423 AVQLEPIFQFDEKEGPYFPFHFFSPTNSYGPSGASISVINSMKEMVKELHANGIEVILEV 482
           AV LEPIF F E++GPYFPFHFFSP + YGPS +  S +NSMK MVK+LH+ GIEV+LEV
Sbjct: 421 AVLLEPIFSFSEQKGPYFPFHFFSPMDIYGPSNSLESAVNSMKVMVKKLHSEGIEVLLEV 480

Query: 483 VFTHTAVDGALQGIDDSSYYFVNRVANLEGKSVLNCNYPIVQQLLLDSLRYWVTEFHIDG 542
           VFTHTA  GAL+GIDDSSYY+  R  +L+ KS LNCNYP+VQQL+L+SLRYWVTEFH+DG
Sbjct: 481 VFTHTADSGALRGIDDSSYYYKGRANDLDSKSYLNCNYPVVQQLVLESLRYWVTEFHVDG 540

Query: 543 FCFINASSLLRGHHGELLSRPPFVEAVAFDPLLSKTKLVADFWDPQDLVSKETHFPHWKR 602
           FCFINASSLLRG HGE LSRPP VEA+AFDPLL++TKL+AD WDP +++ KE  FPHWKR
Sbjct: 541 FCFINASSLLRGVHGEQLSRPPLVEAIAFDPLLAETKLIADCWDPLEMMPKEVRFPHWKR 600

Query: 603 WAEVNSKFCNDIRDFFRGEGLISSLATRLCGSGDVFSDGRGPAFSFNFIARNVGLPLVDL 662
           WAE+N+++C ++R+F RG G++S LATR+CGSGDVF+DGRGPAFSFN+I+RN GL LVD+
Sbjct: 601 WAELNTRYCRNVRNFLRGRGVLSDLATRICGSGDVFTDGRGPAFSFNYISRNSGLSLVDI 660

Query: 663 VSFSDSKLASELSSNCGEEGPTSNIEVLEKRLKQIRNFIFVLFVSLGVPVLNMGDECGQS 722
           VSFS  +LASELS NCGEEG T+   VL++RLKQIRNF+F+ ++SLGVPVLNMGDECG S
Sbjct: 661 VSFSGPELASELSWNCGEEGATNKSAVLQRRLKQIRNFLFIQYISLGVPVLNMGDECGIS 720

Query: 723 SGGSVASNDRRSFNWDLLKTDFGTQTTQFIAFFSSFRSRRFDLFQSRNFLKGENIDWFDN 782
           + GS     R+ F+W+LL + FGTQ TQFI+F +S R+RR D+FQ R+FLK ENI W+ N
Sbjct: 721 TRGSPLLESRKPFDWNLLASAFGTQITQFISFMTSVRARRSDVFQRRDFLKPENIVWYAN 780

Query: 783 NQSSPQWEDPSCKFLAVMLR--ADKEENGSITENAKPRS-DIFMAFNASDQSESVALPEP 842
           +Q++P+WEDP+ KFLA+ ++  +++EE  S+ E  +P+S D+F+ FNASD  ESV LP  
Sbjct: 781 DQTTPKWEDPASKFLALEIKSESEEEETASLAEPNEPKSNDLFIGFNASDHPESVVLPSL 840

Query: 843 SEGTSWFRLVDTALSFPGFFSFNGELV----PTTGSVTYEMQAHSCALFE 882
            +G+ W RLVDTAL FPGFFS  GE V    P    V YEM+ +SC LFE
Sbjct: 841 PDGSKWRRLVDTALPFPGFFSVEGETVVAEEPLQQLVVYEMKPYSCTLFE 876

BLAST of Cla97C09G169430 vs. ExPASy Swiss-Prot
Match: Q6AU80 (Isoamylase 2, chloroplastic OS=Oryza sativa subsp. japonica OX=39947 GN=ISA2 PE=1 SV=1)

HSP 1 Score: 749.2 bits (1933), Expect = 5.3e-215
Identity = 372/716 (51.96%), Postives = 495/716 (69.13%), Query Frame = 0

Query: 185 AVELEFDAKHTPFYLSFLLKYSLGVDSGSSEIRSHRKTSFPVPVGFGRGYPSPLGLSISG 244
           A EL FD    PFYLSFLL      D+  +EIR+HRKTSF VPVG G G P+PLG+SISG
Sbjct: 105 AAELSFDPTRAPFYLSFLL-----TDASGAEIRTHRKTSFRVPVGVGPGSPAPLGMSISG 164

Query: 245 DGSINFSIFSSTAESVVLCLY----NDSTSEKPVLELDLDPYINKSGNIWHASFEGASKF 304
           DG++NF+++S  A +V L LY         ++P LE+DLDPYI+++GN+WH S      +
Sbjct: 165 DGAVNFAVYSKNANAVSLYLYAAAVGGGGGDEPALEIDLDPYIHRTGNVWHVSLASVDGY 224

Query: 305 VSYGYRCKGSKFHENQDGVEVSQIVVDPYAKIL--TASIPKARGQGLGLPS-NLLGQICK 364
           VSY + C G           + + ++DPYAK++    S      +G+  PS      +  
Sbjct: 225 VSYAFCCGG-----------IRRPLLDPYAKVIGDFVSSNSVYDEGVTAPSMRCFASLAI 284

Query: 365 VPAFDWDGEVRPNLPMEKLFVYRLNVKHFTIDKSSQLPADIAGTFSGLTKKLLHFKNLGV 424
            P+++W  +  P LP+EKL VYR NV  FT D+SS LP D AGTF+GL+ K+ HF++LGV
Sbjct: 285 APSYNWGRDRHPRLPLEKLVVYRANVALFTKDRSSGLPDDAAGTFTGLSAKVEHFRSLGV 344

Query: 425 NAVQLEPIFQFDEKEGPYFPFHFFSPTNSYGPSGASISVINSMKEMVKELHANGIEVILE 484
           NA+ LEP+F F + +GPYFP+HFFSP N Y   G S+S I SMK+MV+ +H NGIEV+LE
Sbjct: 345 NAILLEPVFPFHQVKGPYFPYHFFSPMNLYSSKGLSVSAIKSMKDMVRVMHRNGIEVLLE 404

Query: 485 VVFTHTAVDG------ALQGIDDSSYYFVNRVANLEGKSVLNCNYPIVQQLLLDSLRYWV 544
           VVFTHTA         +++GID+SSYY  N +A  +  S+LNCN+P+ Q+L+LDSLR+WV
Sbjct: 405 VVFTHTAEGESECQTISMRGIDNSSYYIANGIAGCKA-SILNCNHPVTQKLILDSLRHWV 464

Query: 545 TEFHIDGFCFINASSLLRGHHGELLSRPPFVEAVAFDPLLSKTKLVADFWDPQDLVSKET 604
            +FH+DGFCFINA  L+RG  GE LSRPP +EA+ FDP+LS TK++AD W P D+ + + 
Sbjct: 465 LDFHVDGFCFINAPFLVRGPGGEYLSRPPLLEAITFDPVLSMTKIIADPWSPLDISNVQF 524

Query: 605 HFPHWKRWAEVNSKFCNDIRDFFRGEGLISSLATRLCGSGDVFSDGRGPAFSFNFIARNV 664
            FPHWKRWAEVN++F  D+R F + E LIS LATRLCGSGD+FS  RGPAFSFN ++RN 
Sbjct: 525 PFPHWKRWAEVNTRFSIDVRKFLKREALISDLATRLCGSGDLFST-RGPAFSFNHVSRNS 584

Query: 665 GLPLVDLVSFSDSKLASELSSNCGEEGPTSNIEVLEKRLKQIRNFIFVLFVSLGVPVLNM 724
           GL LVDLVSFS+  L SE S NCGEEGP+ N  VL+ RL+QIRNF+F+LFVSLGVPVLNM
Sbjct: 585 GLSLVDLVSFSNDDLLSESSWNCGEEGPSENSAVLQTRLRQIRNFLFILFVSLGVPVLNM 644

Query: 725 GDECGQSSGGSVASNDRRSFNWDLLKTDFGTQTTQFIAFFSSFRSRRFDLFQSRNFLKGE 784
           GDECG S+ GSV+  DR   NW  +KT F  + T FI+F ++ RSRR D+FQ R FLK E
Sbjct: 645 GDECGHSAAGSVSYKDRGPLNWRGMKTTFVKEVTGFISFLTALRSRRGDIFQRREFLKLE 704

Query: 785 NIDWFDNNQSSPQWEDPSCKFLAVMLRADKEENGSITENAKPRSDIFMAFNASDQSESVA 844
           NI W+ ++   P W+DP+  FL + + A+ +E  +       R D+++ FNA+++S S A
Sbjct: 705 NIHWYGSDLCEPGWDDPTSNFLCMHINAEVDEMAA----DSVRGDLYICFNANEESVSAA 764

Query: 845 LPEPSEGTSWFRLVDTALSFPGFFS--FNGELVPTTGSVTYEMQAHSCALFEAKSS 886
           LP  +EG+ W RLVDT+L+FPGFF+   N ++    G  +Y ++AH+C LFE+KS+
Sbjct: 765 LPALAEGSVWLRLVDTSLAFPGFFATESNPKVQQVPGLSSYHVEAHTCVLFESKSA 798

BLAST of Cla97C09G169430 vs. ExPASy Swiss-Prot
Match: Q9M0S5 (Isoamylase 3, chloroplastic OS=Arabidopsis thaliana OX=3702 GN=ISA3 PE=1 SV=2)

HSP 1 Score: 367.1 bits (941), Expect = 5.7e-100
Identity = 246/710 (34.65%), Postives = 354/710 (49.86%), Query Frame = 0

Query: 233 GYPSPLGLSISGDGSINFSIFSSTAESVVLCLY-----NDSTSEKPVLELDLDPYINKSG 292
           G  SPLG+S   D  INF++FS  A SV LCL       D T +  ++EL LDP +NK+G
Sbjct: 88  GEVSPLGVS-QVDKGINFALFSQNATSVTLCLSLSQSGKDDTDDDGMIELVLDPSVNKTG 147

Query: 293 NIWHASFEGAS-KFVSYGYRCKGSKFHENQDGVEVSQIVVDPYAKILTASIPKARGQGLG 352
           + WH   E      V YGYR  G    +     + S +++DPYAK++            G
Sbjct: 148 DTWHICVEDLPLNNVLYGYRVDGPGEWQQGHRFDRSILLLDPYAKLVKGH------SSFG 207

Query: 353 LPSNLLGQI-----CKVPAFDWDGEVR-PNLPMEKLFVYRLNVKHFTIDKSSQLPADIAG 412
             S    Q       +   FDW  + + PN+P + L +Y +NV+ FT D+SS +   I G
Sbjct: 208 DSSQKFAQFYGTYDFESSPFDWGDDYKFPNIPEKDLVIYEMNVRAFTADESSGMDPAIGG 267

Query: 413 TFSGLTKKLLHFKNLGVNAVQLEPIFQFDEKE--------------GPYFPFHFFSPTNS 472
           ++ G  +K+ H ++LG+NAV+L P+F+FDE E                Y   +FF+P + 
Sbjct: 268 SYLGFIEKIPHLQDLGINAVELLPVFEFDELELQRRSNPRDHMVNTWGYSTVNFFAPMSR 327

Query: 473 YGP-SGASISVINSMKEMVKELHANGIEVILEVVFTHT-------AVDGALQGIDDSSYY 532
           Y    G  I      KEMVK LH+ GIEVIL+VV+ HT           + +GID+  YY
Sbjct: 328 YASGEGDPIKASKEFKEMVKALHSAGIEVILDVVYNHTNEADDKYPYTTSFRGIDNKVYY 387

Query: 533 FV---NRVANLEG-KSVLNCNYPIVQQLLLDSLRYWVTEFHIDGFCFINASSLLRGHHGE 592
            +   N++ N  G  + LNCN+P+V +L+LDSLR+WVTE+H+DGF F  AS L R   G 
Sbjct: 388 MLDPNNQLLNFSGCGNTLNCNHPVVMELILDSLRHWVTEYHVDGFRFDLASVLCRATDGS 447

Query: 593 LLSRPPFVEAVAFDPLLSKTKLVADFWDPQD--LVSKETHFPHWKRWAEVNSKFCNDIRD 652
            LS PP + A+A D +LS+ K++A+ WD     LV K   FP+W RWAE N  + +D+R 
Sbjct: 448 PLSAPPLIRAIAKDSVLSRCKIIAEPWDCGGLYLVGK---FPNWDRWAEWNGMYRDDVRR 507

Query: 653 FFRGE-GLISSLATRLCGSGDVFS-DGRGPAFSFNFIARNVGLPLVDLVSFS-------- 712
           F +G+ G+  S ATR+ GS D++  + R P    NF+  + G  L DLVS++        
Sbjct: 508 FIKGDSGMKGSFATRVSGSSDLYQVNQRKPYHGVNFVIAHDGFTLRDLVSYNFKHNEANG 567

Query: 713 ---DSKLASELSSNCGEEGPTSNIEVLEKRLKQIRNFIFVLFVSLGVPVLNMGDECGQSS 772
              +       S NCG EG T +  +   R +Q++NF   L +S G P++ MGDE G + 
Sbjct: 568 EGGNDGCNDNHSWNCGFEGETGDAHIKSLRTRQMKNFHLALMISQGTPMMLMGDEYGHTR 627

Query: 773 GGSVAS----NDRRSFNWDLLKTDFGTQTTQFIAFFSSFRSRRFD--LFQSRNFLKGENI 832
            G+  S        +F W     +   +      FFS     R    + +  NFL    I
Sbjct: 628 YGNNNSYGHDTSLNNFQW----KELDAKKQNHFRFFSEVIKFRHSHHVLKHENFLTQGEI 687

Query: 833 DWFDNNQSSPQWEDPSCKFLAVMLRADKEENGSITENAKPRSDIFMAFNASDQSESVALP 884
            W ++N     W++   KFLA  L            +     DI++AFNA D      +P
Sbjct: 688 TWHEDN-----WDNSESKFLAFTL-----------HDGIGGRDIYVAFNAHDYFVKALIP 747

BLAST of Cla97C09G169430 vs. ExPASy Swiss-Prot
Match: B9G434 (Isoamylase 3, chloroplastic OS=Oryza sativa subsp. japonica OX=39947 GN=ISA3 PE=2 SV=1)

HSP 1 Score: 366.3 bits (939), Expect = 9.7e-100
Identity = 252/725 (34.76%), Postives = 367/725 (50.62%), Query Frame = 0

Query: 214 SEIRSHRKTSFPVPVGFGRGYPSPLGLSISGDGSINFSIFSSTAESVVLCLY-----NDS 273
           +E R    +   +P  +  G   PLG+S   +G +NF++FS  A SV+LCL       + 
Sbjct: 84  TEERGCTMSDTEMPFKYSSGKAFPLGVS-QVEGGLNFALFSQHASSVILCLKLPGRGTED 143

Query: 274 TSEKPVLELDLDPYINKSGNIWHASFEG-ASKFVSYGYRCKGSKFHENQDGVEVSQIVVD 333
                V+E  LD   NK+G+IWH   EG  +  V YGYR  G +  +     + S +++D
Sbjct: 144 EKGADVVEFVLDQQKNKTGDIWHVIVEGLPASGVLYGYRVGGPQGWDQGHRFDSSTVLLD 203

Query: 334 PYAKILTASIPKARGQGLGLPSNLLGQI-CKVPAFDWDGEVR-PNLPMEKLFVYRLNVKH 393
           PYAK+++    K  G      S   G        FDW  + R PNLP   L +Y +NV+ 
Sbjct: 204 PYAKLVSGR--KYFGVAEEKSSQHFGTYDFDSSPFDWGDDYRLPNLPEADLVIYEMNVRA 263

Query: 394 FTIDKSSQLPADIAGTFSGLTKKLLHFKNLGVNAVQLEPIFQFDEKEGPYFP-------- 453
           FT D+SS L +   G++ GL  K+ H   LGVNAV+L P+F++DE E   +P        
Sbjct: 264 FTADESSGLDSTSRGSYLGLIDKIPHLLELGVNAVELLPVFEYDELEFKRYPNPRDHMVN 323

Query: 454 ------FHFFSPTNSYGPSGAS-ISVINSMKEMVKELHANGIEVILEVVFTHT--AVDG- 513
                  +FF+P + Y  +G   ++    +K+MVKELH  GIEVIL+VV+ HT  A D  
Sbjct: 324 TWGYSTINFFAPMSRYASAGGGPVAASKELKQMVKELHKAGIEVILDVVYNHTNEADDAH 383

Query: 514 ----ALQGIDDSSYYFVNRVANLE------GKSVLNCNYPIVQQLLLDSLRYWVTEFHID 573
               + +GID+  YY ++   N E        + LNCN+P+V++L+LDSLR+WV E+HID
Sbjct: 384 PYMTSFRGIDNKVYYMLDLNKNAELLNFSGCGNTLNCNHPVVKELILDSLRHWVEEYHID 443

Query: 574 GFCFINASSLLRGHHGELLSRPPFVEAVAFDPLLSKTKLVADFWDPQDLVSKETHFPHWK 633
           GF F  AS L RG  G  L  PP ++ +A D +LS+ K++A+ WD   L      FP+W 
Sbjct: 444 GFRFDLASVLCRGPDGCPLDAPPLIKEIAKDAVLSRCKIIAEPWDCGGLY-LVGRFPNWD 503

Query: 634 RWAEVNSKFCNDIRDFFRGE-GLISSLATRLCGSGDVFS-DGRGPAFSFNFIARNVGLPL 693
           RWAE N K+ +D+R F +G+ G+    ATR+ GS D++  + R P    NF+  + G  L
Sbjct: 504 RWAEWNGKYRDDLRRFIKGDPGMKGVFATRVSGSADLYQVNERKPYHGVNFVIAHDGFTL 563

Query: 694 VDLVSF----SDSK-------LASELSSNCGEEGPTSNIEVLEKRLKQIRNFIFVLFVSL 753
            DLVS+    +D+             S NCG EG T+++ VL  R +Q++NF   L +S 
Sbjct: 564 CDLVSYNLKHNDANGEGGCDGCNDNFSWNCGVEGETNDLNVLSLRSRQMKNFHVALMISQ 623

Query: 754 GVPVLNMGDECGQSSGGSVASNDR----RSFNWDLLKTDFGTQTTQFIAFFSSFRSRRFD 813
           G P++ MGDE G +  G+  S        +F W+ L+     +      FFS     R  
Sbjct: 624 GTPMMLMGDEYGHTRYGNNNSYGHDTCINNFQWEQLE----QRRDGHFRFFSEMIKFRHS 683

Query: 814 --LFQSRNFLKGENIDWFDNNQSSPQWEDPSCKFLAVMLRADKEENGSITENAKPRSDIF 873
             + +   FL   ++ W ++      WE+   KFLA  +  D    G          DI+
Sbjct: 684 NPILRRDRFLNKNDVTWHED-----CWENQESKFLAFTVH-DHNSGG----------DIY 743

Query: 874 MAFNASDQSESVALPEPSEGTSWFRLVDTALSFPGFFSFNGELVPTTGSVTYEMQAHSCA 884
           +AFNA D      +P P     W R+VDT L  P      G  VP TG   Y +  +S  
Sbjct: 744 LAFNAHDYFVDAVIPPPPHHKCWNRVVDTNLESPNDIVPEG--VPFTGP-KYRIAPYSSI 781

BLAST of Cla97C09G169430 vs. ExPASy Swiss-Prot
Match: D0TZF0 (Isoamylase 1, chloroplastic OS=Oryza sativa subsp. japonica OX=39947 GN=ISA1 PE=1 SV=1)

HSP 1 Score: 344.4 bits (882), Expect = 3.9e-93
Identity = 229/683 (33.53%), Postives = 340/683 (49.78%), Query Frame = 0

Query: 233 GYPSPLGLSISGDGSINFSIFSSTAESVVLCLY--NDSTSEKPVLELDLDPYINKSGNIW 292
           G P+PLG + + DG +NF+++S+ A +  LCL+  +D  +++   E+ LDP  N++GN+W
Sbjct: 99  GMPAPLG-ATALDGGVNFAVYSAGASAASLCLFTPDDLEADEVTEEVPLDPLFNRTGNVW 158

Query: 293 HASFEGASKFVSYGYRCKGSKFHENQDGVEVSQIVVDPYAK-ILTASIPKARGQGLGLPS 352
           H   EG    + YGYR  G          +VS +VVDPYAK +++       G G     
Sbjct: 159 HVFIEGELHNMLYGYRFDGMFAPHCGQYFDVSNVVVDPYAKAVISRGEYGVPGPGGDCWP 218

Query: 353 NLLGQI-CKVPAFDWDGEVRPNLPMEKLFVYRLNVKHFTIDKSSQLPADIAGTFSGLTKK 412
            + G I      FDW G++    P + L +Y ++++ FT   SS +  +  GT+ G   K
Sbjct: 219 QMAGMIPLPYSTFDWQGDLPLRYPQKDLVIYEMHLRGFTKHSSSNV--EHPGTYIGAISK 278

Query: 413 LLHFKNLGVNAVQLEPIFQFDEKE----------GPYFPFHFFSPTNSYGPSG---ASIS 472
           L + K LGVN V+L P  +F+E E            Y   +FFSP   Y   G       
Sbjct: 279 LDYLKELGVNCVELMPCHEFNELEYFSCSSKMNFWGYSTINFFSPMIRYSSGGIRNCGRD 338

Query: 473 VINSMKEMVKELHANGIEVILEVVFTHTAVDG------ALQGIDDSSYYFV---NRVANL 532
            IN  K  V+E H  GIEVI++VVF HTA         + +GID+S+YY +       N 
Sbjct: 339 AINEFKTFVREAHKRGIEVIMDVVFNHTAEGNEKGPILSFRGIDNSTYYMLAPKGEFYNY 398

Query: 533 EG-KSVLNCNYPIVQQLLLDSLRYWVTEFHIDGFCFINASSLLRG--------------- 592
            G  +  NCN+P+V++ ++D LRYWVTE H+DGF F  AS + RG               
Sbjct: 399 SGCGNTFNCNHPVVREFIVDCLRYWVTEMHVDGFRFDLASIMTRGCSLWDPVNVYGSPVE 458

Query: 593 ----HHGELLSRPPFVEAVAFDPLLSKTKLVADFWDPQDLVSKETHFPHWKRWAEVNSKF 652
                 G  L+ PP ++ ++ DP+L   KL+A+ WD   L  +   FPHWK W+E N K+
Sbjct: 459 GDMTTTGTPLATPPLIDMISNDPILGDVKLIAEAWDAGGLY-QVGQFPHWKIWSEWNGKY 518

Query: 653 CNDIRDFFRG-EGLISSLATRLCGSGDVF-SDGRGPAFSFNFIARNVGLPLVDLVSFSDS 712
            + +R F +G +G     A  LCGS  ++ + GR P  S NF+  + G  L DLV+++  
Sbjct: 519 RDIVRQFIKGTDGFAGGFAECLCGSPHLYQAGGRKPWHSINFVCAHDGFTLADLVTYNKK 578

Query: 713 KLAS-----------ELSSNCGEEGPTSNIEVLEKRLKQIRNFIFVLFVSLGVPVLNMGD 772
             +S            LS NCGEEG  + + V   R +Q+RNF   L VS GVP+  MGD
Sbjct: 579 YNSSNGEDNRDGENHNLSWNCGEEGEFAGLSVKRLRKRQMRNFFVSLMVSQGVPMFYMGD 638

Query: 773 ECGQSSGG--SVASNDR--RSFNWDLLKTDFGTQTTQFIAFFSSFRSRRFDLFQSRNFLK 832
           E G + GG  +   +D     F WD  K +  +   +F +  + FR ++ +     +F  
Sbjct: 639 EYGHTKGGNNNTYCHDHYVNYFRWD--KKEESSDLQRFCSLMTKFR-KQCESLGLADFPT 698

Query: 833 GENIDWFDNNQSSPQWEDPSCKFLAVMLRADKEENGSITENAKPRSDIFMAFNASDQSES 853
            + + W  +    P W + S +F+A    + K+E          + +I++AFNAS     
Sbjct: 699 AQRLHWHGHQPGKPDWSETS-RFVAF---STKDET---------KGEIYVAFNASHLPAV 758

BLAST of Cla97C09G169430 vs. ExPASy TrEMBL
Match: A0A1S3BRF8 (isoamylase 2, chloroplastic OS=Cucumis melo OX=3656 GN=LOC103492696 PE=4 SV=1)

HSP 1 Score: 1597.0 bits (4134), Expect = 0.0e+00
Identity = 787/884 (89.03%), Postives = 822/884 (92.99%), Query Frame = 0

Query: 3   MATFPSSLVIQPFCVYNCGVTESFKLAVSDHHTYGQKIKYQSGKMDEARMLAHGERKVSF 62
           MATF SSLVIQPFCV+NCGVTES KLAVSDH TYGQK KYQSGKMDEARMLAHGE KV  
Sbjct: 1   MATFLSSLVIQPFCVHNCGVTESLKLAVSDHLTYGQKTKYQSGKMDEARMLAHGESKVGA 60

Query: 63  MKSSHRNLSIAYATSGISVGKSGQRFSTGGKSKEHRKVATYLFRTEFGDLVNVFVEKTGS 122
           +KSSHRNLS AY TSGI VGKSGQR   GGKSKE RKVATYLFRTEFGDLVNVFV KTGS
Sbjct: 61  VKSSHRNLSKAYVTSGILVGKSGQRLGIGGKSKEQRKVATYLFRTEFGDLVNVFVGKTGS 120

Query: 123 GFTVNIEISSVQRANIDETLLLSWGVYRSDSTLVTPNFQSSLPDETIGATETPFLKTSEG 182
            FTVNIEISS+Q  + DETLLLSW VYRSDS  VT NFQSS PD T GATETPF+KTSEG
Sbjct: 121 AFTVNIEISSMQLVSTDETLLLSWRVYRSDSAPVTSNFQSSPPDGTTGATETPFVKTSEG 180

Query: 183 KFAVELEFDAKHTPFYLSFLLKYSLGVDSGSSEIRSHRKTSFPVPVGFGRGYPSPLGLSI 242
           KF+VELEFDAKHTPFYLSF+LK+ +GVDSGSSEIRSHRKTSF VPVGFGRGYPSPLGLSI
Sbjct: 181 KFSVELEFDAKHTPFYLSFVLKFPMGVDSGSSEIRSHRKTSFSVPVGFGRGYPSPLGLSI 240

Query: 243 SGDGSINFSIFSSTAESVVLCLYNDSTSEKPVLELDLDPYINKSGNIWHASFEGASKFVS 302
           SGDGSINFSIFSSTAESVVLCLYNDSTSEKP+LELDLDPYIN+SGNIWHASFEGAS FVS
Sbjct: 241 SGDGSINFSIFSSTAESVVLCLYNDSTSEKPLLELDLDPYINRSGNIWHASFEGASNFVS 300

Query: 303 YGYRCKGSKFHENQDGVEVSQIVVDPYAKILTASIPKARGQGLGLPSNLLGQICKVPAFD 362
           YGY+CKG K HENQDG+EVS+IVVDPYAKIL  SIPK+R QGLG P   LGQI  VPAFD
Sbjct: 301 YGYQCKGLKSHENQDGLEVSRIVVDPYAKILAPSIPKSRDQGLGFPLKFLGQISIVPAFD 360

Query: 363 WDGEVRPNLPMEKLFVYRLNVKHFTIDKSSQLPADIAGTFSGLTKKLLHFKNLGVNAVQL 422
           WDGEVRPNLPMEKLFVYRLNVK FTIDKSSQLPADIAGTFSGLTKKLLH KNLGVNAV L
Sbjct: 361 WDGEVRPNLPMEKLFVYRLNVKRFTIDKSSQLPADIAGTFSGLTKKLLHLKNLGVNAVLL 420

Query: 423 EPIFQFDEKEGPYFPFHFFSPTNSYGPSGASISVINSMKEMVKELHANGIEVILEVVFTH 482
           EPIFQFDEKEGPYFPFHFFSPTN+YGPSGASIS INSMKEMVKELHANG+EVI+EVV+TH
Sbjct: 421 EPIFQFDEKEGPYFPFHFFSPTNNYGPSGASISAINSMKEMVKELHANGVEVIVEVVYTH 480

Query: 483 TAVDGALQGIDDSSYYFVNRVANLEGKSVLNCNYPIVQQLLLDSLRYWVTEFHIDGFCFI 542
           T+VDGALQGIDDSSYYF NRVANLE +S LNCNYPIVQQLLLDSLRYWVTEFH+DGFCF+
Sbjct: 481 TSVDGALQGIDDSSYYFANRVANLEERSALNCNYPIVQQLLLDSLRYWVTEFHVDGFCFV 540

Query: 543 NASSLLRGHHGELLSRPPFVEAVAFDPLLSKTKLVADFWDPQDLVSKETHFPHWKRWAEV 602
           NAS LL+GHHGELLSRPPFVEA+AFDP+LSKTKLVADFWDPQ+LVSKET FPHWKRWAEV
Sbjct: 541 NASFLLQGHHGELLSRPPFVEAIAFDPILSKTKLVADFWDPQELVSKETRFPHWKRWAEV 600

Query: 603 NSKFCNDIRDFFRGEGLISSLATRLCGSGDVFSDGRGPAFSFNFIARNVGLPLVDLVSFS 662
           NSKFC+DIRDFFRGEGLISSLATRLCGSGDVFSDGRGPAFSFNFIARNVGLPLVDLVSFS
Sbjct: 601 NSKFCSDIRDFFRGEGLISSLATRLCGSGDVFSDGRGPAFSFNFIARNVGLPLVDLVSFS 660

Query: 663 DSKLASELSSNCGEEGPTSNIEVLEKRLKQIRNFIFVLFVSLGVPVLNMGDECGQSSGGS 722
           +S LASELS NCGEEGPT+ ++VLEKRLKQIRNFIFVLFVSLGVPVLNMGDECGQSSGGS
Sbjct: 661 NSNLASELSWNCGEEGPTNTLKVLEKRLKQIRNFIFVLFVSLGVPVLNMGDECGQSSGGS 720

Query: 723 VASNDRRSFNWDLLKTDFGTQTTQFIAFFSSFRSRRFDLFQSRNFLKGENIDWFDNNQSS 782
           VA NDRRSFNWDLLKTDFGTQTTQFIAF SSFRSRRFDLFQSRNFLKGENIDWFDNNQS 
Sbjct: 721 VAFNDRRSFNWDLLKTDFGTQTTQFIAFLSSFRSRRFDLFQSRNFLKGENIDWFDNNQSP 780

Query: 783 PQWEDPSCKFLAVMLRADKEENGSITENAKPRSDIFMAFNASDQSESVALPEPSEGTSWF 842
           PQWED SCKFLAVMLRADKEEN SITEN K RSDIFMAFNASDQSESVALPEPSEGTSWF
Sbjct: 781 PQWEDASCKFLAVMLRADKEENESITENPKTRSDIFMAFNASDQSESVALPEPSEGTSWF 840

Query: 843 RLVDTALSFPGFFSFNGELVPTTGSVTYEMQAHSCALFEAKSSN 887
           R+VDTAL FPGFFS +GELVP TGSVTYEMQAHSCALFEAKS+N
Sbjct: 841 RVVDTALPFPGFFSSDGELVPMTGSVTYEMQAHSCALFEAKSAN 884

BLAST of Cla97C09G169430 vs. ExPASy TrEMBL
Match: A0A0A0K551 (Aamy domain-containing protein OS=Cucumis sativus OX=3659 GN=Csa_7G392380 PE=4 SV=1)

HSP 1 Score: 1596.6 bits (4133), Expect = 0.0e+00
Identity = 782/885 (88.36%), Postives = 824/885 (93.11%), Query Frame = 0

Query: 3   MATFPSSLVIQPFCVYNCGVTESFKLAVSDHHTYGQKIKYQSGKMDEARMLAHGERKVSF 62
           MATF SSLVIQPFCVYNCGVTES KLA SDH TYGQK KYQ GKMDEARMLAHGE KV  
Sbjct: 1   MATFLSSLVIQPFCVYNCGVTESPKLAASDHLTYGQKTKYQFGKMDEARMLAHGENKVGA 60

Query: 63  MKSSHRNLSIAYATSGISVGKSGQRFSTGGKSKEHRKVATYLFRTEFGDLVNVFVEKTGS 122
           +KSSHRNLS  YA SGISVGKSGQR   GGKSKE R+VATYLFRTEFGDLVNVFV K GS
Sbjct: 61  VKSSHRNLSKTYAKSGISVGKSGQRLGIGGKSKEQRRVATYLFRTEFGDLVNVFVGKKGS 120

Query: 123 GFTVNIEISSVQRANIDETLLLSWGVYRSDSTLVTPNFQSSLPDETIGATETPFLKTSEG 182
            FTVNIE+ S+Q  +IDE LLLSWGVYRSDS LVTPNF+SS PDET GATETPF+KTSEG
Sbjct: 121 TFTVNIEVPSMQLVSIDEALLLSWGVYRSDSALVTPNFESSPPDETTGATETPFVKTSEG 180

Query: 183 KFAVELEFDAKHTPFYLSFLLKYSLGVDSGSSEIRSHRKTSFPVPVGFGRGYPSPLGLSI 242
           KF+VELEFDAKHTPFYLSF+LKY +GVDSGSSEIRSH+KTSF VPVGFGRGYPSPLGLSI
Sbjct: 181 KFSVELEFDAKHTPFYLSFVLKYPMGVDSGSSEIRSHKKTSFSVPVGFGRGYPSPLGLSI 240

Query: 243 SGDGSINFSIFSSTAESVVLCLYNDSTSEKPVLELDLDPYINKSGNIWHASFEGASKFVS 302
           SGDGS+NFSIFSS+AES+VLCLYNDSTSEKP+LELDLDPYIN+SGNIWHASFEGASKFVS
Sbjct: 241 SGDGSVNFSIFSSSAESLVLCLYNDSTSEKPLLELDLDPYINRSGNIWHASFEGASKFVS 300

Query: 303 YGYRCKGSKFHENQDGVEVSQIVVDPYAKILTASIPKARGQGLGLPSNLLGQICKVPAFD 362
           YGY+CKGSK HENQDG+EVS+IVVDPYAKIL  SIPK+ GQGLGLPS  LGQI KVP FD
Sbjct: 301 YGYQCKGSKSHENQDGLEVSRIVVDPYAKILAPSIPKSSGQGLGLPSKFLGQISKVPTFD 360

Query: 363 WDGEVRPNLPMEKLFVYRLNVKHFTIDKSSQLPADIAGTFSGLTKKLLHFKNLGVNAVQL 422
           WDGEV PNLPMEKLFVYRLNV+ FT+DKSSQLPADIAGTFSGLTKKLLHFKNLGVNAV L
Sbjct: 361 WDGEVHPNLPMEKLFVYRLNVERFTMDKSSQLPADIAGTFSGLTKKLLHFKNLGVNAVLL 420

Query: 423 EPIFQFDEKEGPYFPFHFFSPTNSYGPSGASISVINSMKEMVKELHANGIEVILEVVFTH 482
           EPIFQFDEKEGPYFPFHFFSPTN+YGPSGASIS INSMKEMVKELHANG+EVILEVV+TH
Sbjct: 421 EPIFQFDEKEGPYFPFHFFSPTNNYGPSGASISAINSMKEMVKELHANGVEVILEVVYTH 480

Query: 483 TAVDGALQGIDDSSYYFVNRVANLEGKSVLNCNYPIVQQLLLDSLRYWVTEFHIDGFCFI 542
           T+ +GALQGIDDSSYYF NRVANLE KS LNCNYPIVQQLLLDSLRYWVTEFH+DGFCF+
Sbjct: 481 TSGNGALQGIDDSSYYFTNRVANLEEKSALNCNYPIVQQLLLDSLRYWVTEFHVDGFCFV 540

Query: 543 NASSLLRGHHGELLSRPPFVEAVAFDPLLSKTKLVADFWDPQDLVSKETHFPHWKRWAEV 602
           NAS LLRGHHGELLSRPPFVEA+AFDPLLSKTKLVADFWDPQ+L SKET FPHWKRWAEV
Sbjct: 541 NASFLLRGHHGELLSRPPFVEAIAFDPLLSKTKLVADFWDPQELESKETRFPHWKRWAEV 600

Query: 603 NSKFCNDIRDFFRGEGLISSLATRLCGSGDVFSDGRGPAFSFNFIARNVGLPLVDLVSFS 662
           NSKFC+DIRDFFRGEGLISSLATRLCGSGDVFSDGRGPAFSFNFIARNVGLPLVDLVSFS
Sbjct: 601 NSKFCSDIRDFFRGEGLISSLATRLCGSGDVFSDGRGPAFSFNFIARNVGLPLVDLVSFS 660

Query: 663 DSKLASELSSNCGEEGPTSNIEVLEKRLKQIRNFIFVLFVSLGVPVLNMGDECGQSSGGS 722
           +S LASELS NCGEEGPTSN++VLEKRLKQIRNFIFVLFVSLGVPVLNMGDECGQSSGGS
Sbjct: 661 NSNLASELSWNCGEEGPTSNLKVLEKRLKQIRNFIFVLFVSLGVPVLNMGDECGQSSGGS 720

Query: 723 VASNDRRSFNWDLLKTDFGTQTTQFIAFFSSFRSRRFDLFQSRNFLKGENIDWFDNNQSS 782
           VA ND+RSFNWDLLKTDFGTQTTQFIAF SSFRSRRFDLFQ+RNFLKGENIDWFDNNQS 
Sbjct: 721 VAFNDKRSFNWDLLKTDFGTQTTQFIAFLSSFRSRRFDLFQNRNFLKGENIDWFDNNQSP 780

Query: 783 PQWEDPSCKFLAVMLRADKEENGSITENAKPRSDIFMAFNASDQSESVALPEPSEGTSWF 842
           PQWED SCKFLAVMLRADKEEN SITEN K RS+IFM FNASDQSESVALPEP EGTSWF
Sbjct: 781 PQWEDASCKFLAVMLRADKEENESITENPKTRSNIFMVFNASDQSESVALPEPLEGTSWF 840

Query: 843 RLVDTALSFPGFFSFNGELVPTTGSVTYEMQAHSCALFEAKSSND 888
           R+VDTAL FPGFFS +GELVP TGSVTYE+QAHSCALFEAKS+ND
Sbjct: 841 RVVDTALPFPGFFSSDGELVPMTGSVTYEIQAHSCALFEAKSAND 885

BLAST of Cla97C09G169430 vs. ExPASy TrEMBL
Match: A0A5A7UR47 (Isoamylase 2 OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold328G00230 PE=4 SV=1)

HSP 1 Score: 1595.5 bits (4130), Expect = 0.0e+00
Identity = 786/884 (88.91%), Postives = 821/884 (92.87%), Query Frame = 0

Query: 3   MATFPSSLVIQPFCVYNCGVTESFKLAVSDHHTYGQKIKYQSGKMDEARMLAHGERKVSF 62
           MATF SSLVIQPFCV+NCGVTES KLAVSDH TYGQK KYQSGKMDEARMLAHGE KV  
Sbjct: 1   MATFLSSLVIQPFCVHNCGVTESLKLAVSDHLTYGQKTKYQSGKMDEARMLAHGESKVGA 60

Query: 63  MKSSHRNLSIAYATSGISVGKSGQRFSTGGKSKEHRKVATYLFRTEFGDLVNVFVEKTGS 122
           +KSSHRNLS AY TSGISVGKSGQR   GGKSKE RKVATYLFRTEFGDLVNVFV KTGS
Sbjct: 61  VKSSHRNLSKAYVTSGISVGKSGQRLGIGGKSKEQRKVATYLFRTEFGDLVNVFVGKTGS 120

Query: 123 GFTVNIEISSVQRANIDETLLLSWGVYRSDSTLVTPNFQSSLPDETIGATETPFLKTSEG 182
            FTVNIEISS+Q  + DETLLLSW VYRSDS  VT NFQSS PD T GATETPF+KTSEG
Sbjct: 121 AFTVNIEISSMQLVSTDETLLLSWRVYRSDSAPVTSNFQSSPPDGTTGATETPFVKTSEG 180

Query: 183 KFAVELEFDAKHTPFYLSFLLKYSLGVDSGSSEIRSHRKTSFPVPVGFGRGYPSPLGLSI 242
           KF+VELEFDAKHTPFYLSF+LK+ +GVDSGSSEIRSHRKTSF VPVGFGRGYPSP GLSI
Sbjct: 181 KFSVELEFDAKHTPFYLSFVLKFPMGVDSGSSEIRSHRKTSFSVPVGFGRGYPSPSGLSI 240

Query: 243 SGDGSINFSIFSSTAESVVLCLYNDSTSEKPVLELDLDPYINKSGNIWHASFEGASKFVS 302
           SGDGSINFSIFSSTAESVVLCLYNDSTSEKP+LELDLDPYIN+SGNIWHASFEG S FVS
Sbjct: 241 SGDGSINFSIFSSTAESVVLCLYNDSTSEKPLLELDLDPYINRSGNIWHASFEGVSNFVS 300

Query: 303 YGYRCKGSKFHENQDGVEVSQIVVDPYAKILTASIPKARGQGLGLPSNLLGQICKVPAFD 362
           YGY+CKG K HENQDG+EVS+IVVDPYAKIL  SIPK+R QGLG P   LGQI  VPAFD
Sbjct: 301 YGYQCKGLKSHENQDGLEVSRIVVDPYAKILAPSIPKSRDQGLGFPLKFLGQISIVPAFD 360

Query: 363 WDGEVRPNLPMEKLFVYRLNVKHFTIDKSSQLPADIAGTFSGLTKKLLHFKNLGVNAVQL 422
           WDGEVRPNLPMEKLFVYRLNVK FTIDKSSQLPADIAGTFSGLTKKLLH KNLGVNAV L
Sbjct: 361 WDGEVRPNLPMEKLFVYRLNVKRFTIDKSSQLPADIAGTFSGLTKKLLHLKNLGVNAVLL 420

Query: 423 EPIFQFDEKEGPYFPFHFFSPTNSYGPSGASISVINSMKEMVKELHANGIEVILEVVFTH 482
           EPIFQFDEKEGPYFPFHFFSPTN+YGPSGASIS INSMKEMVKELHANG+EVI+EVV+TH
Sbjct: 421 EPIFQFDEKEGPYFPFHFFSPTNNYGPSGASISAINSMKEMVKELHANGVEVIVEVVYTH 480

Query: 483 TAVDGALQGIDDSSYYFVNRVANLEGKSVLNCNYPIVQQLLLDSLRYWVTEFHIDGFCFI 542
           T+VDGALQGIDDSSYYF NRVANLE +S LNCNYPIVQQLLLDSLRYWVTEFH+DGFCF+
Sbjct: 481 TSVDGALQGIDDSSYYFANRVANLEERSALNCNYPIVQQLLLDSLRYWVTEFHVDGFCFV 540

Query: 543 NASSLLRGHHGELLSRPPFVEAVAFDPLLSKTKLVADFWDPQDLVSKETHFPHWKRWAEV 602
           NAS LL+GHHGELLSRPPFVEA+AFDP+LSKTKLVADFWDPQ+LVSKET FPHWKRWAEV
Sbjct: 541 NASFLLQGHHGELLSRPPFVEAIAFDPILSKTKLVADFWDPQELVSKETRFPHWKRWAEV 600

Query: 603 NSKFCNDIRDFFRGEGLISSLATRLCGSGDVFSDGRGPAFSFNFIARNVGLPLVDLVSFS 662
           NSKFC+DIRDFFRGEGLISSLATRLCGSGDVFSDGRGPAFSFNFIARNVGLPLVDLVSFS
Sbjct: 601 NSKFCSDIRDFFRGEGLISSLATRLCGSGDVFSDGRGPAFSFNFIARNVGLPLVDLVSFS 660

Query: 663 DSKLASELSSNCGEEGPTSNIEVLEKRLKQIRNFIFVLFVSLGVPVLNMGDECGQSSGGS 722
           +S LASELS NCGEEGPT+ ++VLEKRLKQIRNFIFVLFVSLGVPVLNMGDECGQSSGGS
Sbjct: 661 NSNLASELSWNCGEEGPTNTLKVLEKRLKQIRNFIFVLFVSLGVPVLNMGDECGQSSGGS 720

Query: 723 VASNDRRSFNWDLLKTDFGTQTTQFIAFFSSFRSRRFDLFQSRNFLKGENIDWFDNNQSS 782
           VA NDRRSFNWDLLKTDFGTQTTQFIAF SSFRSRRFDLFQSRNFLKGENIDWFDNNQS 
Sbjct: 721 VAFNDRRSFNWDLLKTDFGTQTTQFIAFLSSFRSRRFDLFQSRNFLKGENIDWFDNNQSP 780

Query: 783 PQWEDPSCKFLAVMLRADKEENGSITENAKPRSDIFMAFNASDQSESVALPEPSEGTSWF 842
           PQWED SCKFLAVMLRADKEEN SITEN K RSDIFMAFNASDQSESVALPEPSEGTSWF
Sbjct: 781 PQWEDASCKFLAVMLRADKEENESITENPKTRSDIFMAFNASDQSESVALPEPSEGTSWF 840

Query: 843 RLVDTALSFPGFFSFNGELVPTTGSVTYEMQAHSCALFEAKSSN 887
           R+VDTAL FPGFFS +GELVP TGSVTYEMQAHSCALFEAKS+N
Sbjct: 841 RVVDTALPFPGFFSSDGELVPMTGSVTYEMQAHSCALFEAKSAN 884

BLAST of Cla97C09G169430 vs. ExPASy TrEMBL
Match: A0A6J1GPU3 (isoamylase 2, chloroplastic isoform X1 OS=Cucurbita moschata OX=3662 GN=LOC111456039 PE=4 SV=1)

HSP 1 Score: 1563.1 bits (4046), Expect = 0.0e+00
Identity = 768/885 (86.78%), Postives = 814/885 (91.98%), Query Frame = 0

Query: 3   MATFPSSLVIQPFCVYNCGVTESFKLAVSDHHTYGQKIKYQSGKMDEARMLAHGERKVSF 62
           MATF SSL IQPFC YN G TESFKLAVSD+HTYGQK+ YQSGK+DEARM AHGE KV F
Sbjct: 1   MATFLSSL-IQPFCGYNRGGTESFKLAVSDYHTYGQKMNYQSGKLDEARMQAHGESKVGF 60

Query: 63  MKSSHRNLSIAYATSGISVGKSGQRFSTGGKSKEHRKVATYLFRTEFGDLVNVFVEKTGS 122
            KS HRN+  AYA S ISVGKSGQ+F TGGK++E RKVATYLFRTEFGDLV  FV K GS
Sbjct: 61  TKSLHRNVCRAYAPSRISVGKSGQKFGTGGKTEEDRKVATYLFRTEFGDLVYAFVGKRGS 120

Query: 123 GFTVNIEISSVQRANIDETLLLSWGVYRSDSTLVTPNFQSSLPDETIGATETPFLKTSEG 182
           GF  +IEISS++ A+IDETLLLSWGVYRS+S +VTPNFQS LP+ETIGATETPF++TSEG
Sbjct: 121 GFMFSIEISSLKLASIDETLLLSWGVYRSNSAIVTPNFQSILPEETIGATETPFVRTSEG 180

Query: 183 KFAVELEFDAKHTPFYLSFLLKYSLGVDSGSSEIRSHRKTSFPVPVGFGRGYPSPLGLSI 242
           KFAVELEFDAKH PFYLSFLLKY +GVDSGSSEI+SHRKTSF VPVGFGRGYPSPLGLSI
Sbjct: 181 KFAVELEFDAKHDPFYLSFLLKYPVGVDSGSSEIKSHRKTSFSVPVGFGRGYPSPLGLSI 240

Query: 243 SGDGSINFSIFSSTAESVVLCLYNDSTSEKPVLELDLDPYINKSGNIWHASFEGASKFVS 302
           SGDGSINFSIFSSTAESVVLCLYNDSTSEKPVLELDLDPYIN+SGNIWHASFEGAS FVS
Sbjct: 241 SGDGSINFSIFSSTAESVVLCLYNDSTSEKPVLELDLDPYINRSGNIWHASFEGASNFVS 300

Query: 303 YGYRCKGSKFHENQDGVEVSQIVVDPYAKILTASIPKARGQGLGLPSNLLGQICKVPAFD 362
           YGYRC+GSKFHENQD  EVS+I+VDPYAKILTASIPK RGQGLGLPS  LGQIC VPAF 
Sbjct: 301 YGYRCRGSKFHENQDEAEVSRILVDPYAKILTASIPKTRGQGLGLPSKFLGQICNVPAFS 360

Query: 363 WDGEVRPNLPMEKLFVYRLNVKHFTIDKSSQLPADIAGTFSGLTKKLLHFKNLGVNAVQL 422
           WDGEVRPNLPMEKLF+YRLNVKHFT+DKSSQLP D AGTFSGLT+KLLHFK LGVNAV L
Sbjct: 361 WDGEVRPNLPMEKLFIYRLNVKHFTVDKSSQLPIDTAGTFSGLTQKLLHFKKLGVNAVLL 420

Query: 423 EPIFQFDEKEGPYFPFHFFSPTNSYGPSGASISVINSMKEMVKELHANGIEVILEVVFTH 482
           EPIF FDEK+GPYFPFHFFSPTN Y PSGASIS +NS+KEMVKELHANG+EVILEVVFTH
Sbjct: 421 EPIFPFDEKDGPYFPFHFFSPTNIYVPSGASISSLNSVKEMVKELHANGVEVILEVVFTH 480

Query: 483 TAVDGALQGIDDSSYYFVNRVANLEGKSVLNCNYPIVQQLLLDSLRYWVTEFHIDGFCFI 542
           TA+DGALQGIDDS YYFVNRVAN EGKS LNCN+PIVQQLLLDSLR+WVTEFHIDGFCFI
Sbjct: 481 TAMDGALQGIDDSCYYFVNRVANSEGKSALNCNHPIVQQLLLDSLRHWVTEFHIDGFCFI 540

Query: 543 NASSLLRGHHGELLSRPPFVEAVAFDPLLSKTKLVADFWDPQDLVSKETHFPHWKRWAEV 602
           NASSLL+GHHGELLSRPPFVEA+AFDPLLSKTK++ADFWDPQ+ V KET FPHWKRWAE+
Sbjct: 541 NASSLLQGHHGELLSRPPFVEAIAFDPLLSKTKIIADFWDPQESVPKETRFPHWKRWAEL 600

Query: 603 NSKFCNDIRDFFRGEGLISSLATRLCGSGDVFSDGRGPAFSFNFIARNVGLPLVDLVSFS 662
           NSKF NDI++FFRGEGL+SSLATRLCGSGDVFSDGRGPAFSFNFIARNVGLPLVDLVSFS
Sbjct: 601 NSKFSNDIKEFFRGEGLVSSLATRLCGSGDVFSDGRGPAFSFNFIARNVGLPLVDLVSFS 660

Query: 663 DSKLASELSSNCGEEGPTSNIEVLEKRLKQIRNFIFVLFVSLGVPVLNMGDECGQSSGGS 722
            SKLASELS NCGEEGPTSNIEVLEKRLKQIRNFIFVLFVSLGVPVLNMGDECGQSSGGS
Sbjct: 661 SSKLASELSWNCGEEGPTSNIEVLEKRLKQIRNFIFVLFVSLGVPVLNMGDECGQSSGGS 720

Query: 723 VASNDRRSFNWDLLKTDFGTQTTQFIAFFSSFRSRRFDLFQSRNFLKGENIDWFDNNQSS 782
           VA  DR+SFNWD LKTDFGTQTTQFI F SSFRSRRFDLFQSRNFLK ENIDWFDNNQS 
Sbjct: 721 VAFKDRKSFNWDSLKTDFGTQTTQFITFLSSFRSRRFDLFQSRNFLKVENIDWFDNNQSP 780

Query: 783 PQWEDPSCKFLAVMLRADKEENGSITENAKPRSDIFMAFNASDQSESVALPEPSEGTSWF 842
           P WEDPSCKFLAVMLRADKEE+GSITEN KP+SDIFMAFNASDQS SVALPEPSEGT+WF
Sbjct: 781 PDWEDPSCKFLAVMLRADKEESGSITENPKPQSDIFMAFNASDQSVSVALPEPSEGTNWF 840

Query: 843 RLVDTALSFPGFFSFNGELVPTTGSVTYEMQAHSCALFEAKSSND 888
           RLVDTALSFPGFFS +GE VPTT  VTY MQAHSC LFEAKS ND
Sbjct: 841 RLVDTALSFPGFFSSDGEPVPTTSPVTYGMQAHSCTLFEAKSFND 884

BLAST of Cla97C09G169430 vs. ExPASy TrEMBL
Match: A0A6J1GN63 (isoamylase 2, chloroplastic isoform X2 OS=Cucurbita moschata OX=3662 GN=LOC111456039 PE=4 SV=1)

HSP 1 Score: 1540.0 bits (3986), Expect = 0.0e+00
Identity = 761/885 (85.99%), Postives = 805/885 (90.96%), Query Frame = 0

Query: 3   MATFPSSLVIQPFCVYNCGVTESFKLAVSDHHTYGQKIKYQSGKMDEARMLAHGERKVSF 62
           MATF SSL IQPFC YN G TESFKLAVSD+HTYGQK+ YQSGK+DEARM AHGE KV F
Sbjct: 1   MATFLSSL-IQPFCGYNRGGTESFKLAVSDYHTYGQKMNYQSGKLDEARMQAHGESKVGF 60

Query: 63  MKSSHRNLSIAYATSGISVGKSGQRFSTGGKSKEHRKVATYLFRTEFGDLVNVFVEKTGS 122
            KS HRN+  AYA S ISV          GKS+E RKVATYLFRTEFGDLV  FV K GS
Sbjct: 61  TKSLHRNVCRAYAPSRISV----------GKSEEDRKVATYLFRTEFGDLVYAFVGKRGS 120

Query: 123 GFTVNIEISSVQRANIDETLLLSWGVYRSDSTLVTPNFQSSLPDETIGATETPFLKTSEG 182
           GF  +IEISS++ A+IDETLLLSWGVYRS+S +VTPNFQS LP+ETIGATETPF++TSEG
Sbjct: 121 GFMFSIEISSLKLASIDETLLLSWGVYRSNSAIVTPNFQSILPEETIGATETPFVRTSEG 180

Query: 183 KFAVELEFDAKHTPFYLSFLLKYSLGVDSGSSEIRSHRKTSFPVPVGFGRGYPSPLGLSI 242
           KFAVELEFDAKH PFYLSFLLKY +GVDSGSSEI+SHRKTSF VPVGFGRGYPSPLGLSI
Sbjct: 181 KFAVELEFDAKHDPFYLSFLLKYPVGVDSGSSEIKSHRKTSFSVPVGFGRGYPSPLGLSI 240

Query: 243 SGDGSINFSIFSSTAESVVLCLYNDSTSEKPVLELDLDPYINKSGNIWHASFEGASKFVS 302
           SGDGSINFSIFSSTAESVVLCLYNDSTSEKPVLELDLDPYIN+SGNIWHASFEGAS FVS
Sbjct: 241 SGDGSINFSIFSSTAESVVLCLYNDSTSEKPVLELDLDPYINRSGNIWHASFEGASNFVS 300

Query: 303 YGYRCKGSKFHENQDGVEVSQIVVDPYAKILTASIPKARGQGLGLPSNLLGQICKVPAFD 362
           YGYRC+GSKFHENQD  EVS+I+VDPYAKILTASIPK RGQGLGLPS  LGQIC VPAF 
Sbjct: 301 YGYRCRGSKFHENQDEAEVSRILVDPYAKILTASIPKTRGQGLGLPSKFLGQICNVPAFS 360

Query: 363 WDGEVRPNLPMEKLFVYRLNVKHFTIDKSSQLPADIAGTFSGLTKKLLHFKNLGVNAVQL 422
           WDGEVRPNLPMEKLF+YRLNVKHFT+DKSSQLP D AGTFSGLT+KLLHFK LGVNAV L
Sbjct: 361 WDGEVRPNLPMEKLFIYRLNVKHFTVDKSSQLPIDTAGTFSGLTQKLLHFKKLGVNAVLL 420

Query: 423 EPIFQFDEKEGPYFPFHFFSPTNSYGPSGASISVINSMKEMVKELHANGIEVILEVVFTH 482
           EPIF FDEK+GPYFPFHFFSPTN Y PSGASIS +NS+KEMVKELHANG+EVILEVVFTH
Sbjct: 421 EPIFPFDEKDGPYFPFHFFSPTNIYVPSGASISSLNSVKEMVKELHANGVEVILEVVFTH 480

Query: 483 TAVDGALQGIDDSSYYFVNRVANLEGKSVLNCNYPIVQQLLLDSLRYWVTEFHIDGFCFI 542
           TA+DGALQGIDDS YYFVNRVAN EGKS LNCN+PIVQQLLLDSLR+WVTEFHIDGFCFI
Sbjct: 481 TAMDGALQGIDDSCYYFVNRVANSEGKSALNCNHPIVQQLLLDSLRHWVTEFHIDGFCFI 540

Query: 543 NASSLLRGHHGELLSRPPFVEAVAFDPLLSKTKLVADFWDPQDLVSKETHFPHWKRWAEV 602
           NASSLL+GHHGELLSRPPFVEA+AFDPLLSKTK++ADFWDPQ+ V KET FPHWKRWAE+
Sbjct: 541 NASSLLQGHHGELLSRPPFVEAIAFDPLLSKTKIIADFWDPQESVPKETRFPHWKRWAEL 600

Query: 603 NSKFCNDIRDFFRGEGLISSLATRLCGSGDVFSDGRGPAFSFNFIARNVGLPLVDLVSFS 662
           NSKF NDI++FFRGEGL+SSLATRLCGSGDVFSDGRGPAFSFNFIARNVGLPLVDLVSFS
Sbjct: 601 NSKFSNDIKEFFRGEGLVSSLATRLCGSGDVFSDGRGPAFSFNFIARNVGLPLVDLVSFS 660

Query: 663 DSKLASELSSNCGEEGPTSNIEVLEKRLKQIRNFIFVLFVSLGVPVLNMGDECGQSSGGS 722
            SKLASELS NCGEEGPTSNIEVLEKRLKQIRNFIFVLFVSLGVPVLNMGDECGQSSGGS
Sbjct: 661 SSKLASELSWNCGEEGPTSNIEVLEKRLKQIRNFIFVLFVSLGVPVLNMGDECGQSSGGS 720

Query: 723 VASNDRRSFNWDLLKTDFGTQTTQFIAFFSSFRSRRFDLFQSRNFLKGENIDWFDNNQSS 782
           VA  DR+SFNWD LKTDFGTQTTQFI F SSFRSRRFDLFQSRNFLK ENIDWFDNNQS 
Sbjct: 721 VAFKDRKSFNWDSLKTDFGTQTTQFITFLSSFRSRRFDLFQSRNFLKVENIDWFDNNQSP 780

Query: 783 PQWEDPSCKFLAVMLRADKEENGSITENAKPRSDIFMAFNASDQSESVALPEPSEGTSWF 842
           P WEDPSCKFLAVMLRADKEE+GSITEN KP+SDIFMAFNASDQS SVALPEPSEGT+WF
Sbjct: 781 PDWEDPSCKFLAVMLRADKEESGSITENPKPQSDIFMAFNASDQSVSVALPEPSEGTNWF 840

Query: 843 RLVDTALSFPGFFSFNGELVPTTGSVTYEMQAHSCALFEAKSSND 888
           RLVDTALSFPGFFS +GE VPTT  VTY MQAHSC LFEAKS ND
Sbjct: 841 RLVDTALSFPGFFSSDGEPVPTTSPVTYGMQAHSCTLFEAKSFND 874

BLAST of Cla97C09G169430 vs. TAIR 10
Match: AT1G03310.2 (debranching enzyme 1 )

HSP 1 Score: 933.3 bits (2411), Expect = 1.4e-271
Identity = 477/890 (53.60%), Postives = 628/890 (70.56%), Query Frame = 0

Query: 3   MATFPSSLVIQPFCVYNCGVTESFKLAVSDHHT-YGQKIKYQSGKMDEARMLAHGERKVS 62
           MA +  S+ I   C+ N G+T ++K   +   T    KIK  S  +   R    G+   S
Sbjct: 1   MAAWSPSVGIGSCCLNN-GITRTWKFPSARLFTGRKNKIKLGSETLMFTRKRFMGDLVTS 60

Query: 63  FMKSSHRNLSIAYATSGISVGKSGQRFSTGGKSKEHRKVATYLFRTEFGDLVNVFVEKTG 122
            ++S   +   A  T   S+       S   ++ + +KV +Y FRT+ G LV V VEK  
Sbjct: 61  ALQSYQFSKICASKT---SIELREALSSRRAEADDLKKVTSYSFRTKAGALVKVKVEKKR 120

Query: 123 SGFTVNIEISSVQRANIDET-LLLSWGVYRSDSTLVTP-NFQSSLPDETIGATETPFLKT 182
             +++ + +SS++ +  D++ L++ WGVYRSDS+   P +F++S  D     TET F+K+
Sbjct: 121 EKYSILVYVSSLELSGDDKSRLVMVWGVYRSDSSCFLPLDFENSSQDSQTHTTETTFVKS 180

Query: 183 SEGKFAVELEFDAKHTPFYLSFLLKYSLGVDSGSSEIRSHRKTSFPVPVGFGRGYPSPLG 242
           S  +  + LEFD K +PFYLSF LK   G D    E+ +HR T F +PVGF  G+P PLG
Sbjct: 181 SLSELMLGLEFDGKESPFYLSFHLKLVSGRDPDGQEMLTHRDTDFCIPVGFTAGHPLPLG 240

Query: 243 LSIS-GDGSINFSIFSSTAESVVLCLYNDSTSEKPVLELDLDPYINKSGNIWHASFEGAS 302
           LS    D S NFS FS ++ +VVLCLY+DST++KP LELDLDPY+N++G++WHAS +   
Sbjct: 241 LSSGPDDDSWNFSFFSRSSTNVVLCLYDDSTTDKPALELDLDPYVNRTGDVWHASVDNTW 300

Query: 303 KFVSYGYRCKGSKFHENQDGVEVSQIVVDPYAKILTASIPKARGQGLGLPSNLLGQICKV 362
            FV YGYRCK +   +    VE   IV+DPYA ++          G  +    LG + K 
Sbjct: 301 DFVRYGYRCKETAHSKEDVDVEGEPIVLDPYATVV----------GKSVSQKYLGSLSKS 360

Query: 363 PAFDWDGEVRPNLPMEKLFVYRLNVKHFTIDKSSQLPADIAGTFSGLTKKLLHFKNLGVN 422
           P+FDW  +V PN+P+EKL VYRLNVK FT  +SS+LP+++AGTFSG+ +K+ H K LG N
Sbjct: 361 PSFDWGEDVSPNIPLEKLLVYRLNVKGFTQHRSSKLPSNVAGTFSGVAEKVSHLKTLGTN 420

Query: 423 AVQLEPIFQFDEKEGPYFPFHFFSPTNSYGPSGASISVINSMKEMVKELHANGIEVILEV 482
           AV LEPIF F E++GPYFPFHFFSP + YGPS +  S +NSMK MVK+LH+ GIEV+LEV
Sbjct: 421 AVLLEPIFSFSEQKGPYFPFHFFSPMDIYGPSNSLESAVNSMKVMVKKLHSEGIEVLLEV 480

Query: 483 VFTHTAVDGALQGIDDSSYYFVNRVANLEGKSVLNCNYPIVQQLLLDSLRYWVTEFHIDG 542
           VFTHTA  GAL+GIDDSSYY+  R  +L+ KS LNCNYP+VQQL+L+SLRYWVTEFH+DG
Sbjct: 481 VFTHTADSGALRGIDDSSYYYKGRANDLDSKSYLNCNYPVVQQLVLESLRYWVTEFHVDG 540

Query: 543 FCFINASSLLRGHHGELLSRPPFVEAVAFDPLLSKTKLVADFWDPQDLVSKETHFPHWKR 602
           FCFINASSLLRG HGE LSRPP VEA+AFDPLL++TKL+AD WDP +++ KE  FPHWKR
Sbjct: 541 FCFINASSLLRGVHGEQLSRPPLVEAIAFDPLLAETKLIADCWDPLEMMPKEVRFPHWKR 600

Query: 603 WAEVNSKFCNDIRDFFRGEGLISSLATRLCGSGDVFSDGRGPAFSFNFIARNVGLPLVDL 662
           WAE+N+++C ++R+F RG G++S LATR+CGSGDVF+DGRGPAFSFN+I+RN GL LVD+
Sbjct: 601 WAELNTRYCRNVRNFLRGRGVLSDLATRICGSGDVFTDGRGPAFSFNYISRNSGLSLVDI 660

Query: 663 VSFSDSKLASELSSNCGEEGPTSNIEVLEKRLKQIRNFIFVLFVSLGVPVLNMGDECGQS 722
           VSFS  +LASELS NCGEEG T+   VL++RLKQIRNF+F+ ++SLGVPVLNMGDECG S
Sbjct: 661 VSFSGPELASELSWNCGEEGATNKSAVLQRRLKQIRNFLFIQYISLGVPVLNMGDECGIS 720

Query: 723 SGGSVASNDRRSFNWDLLKTDFGTQTTQFIAFFSSFRSRRFDLFQSRNFLKGENIDWFDN 782
           + GS     R+ F+W+LL + FGTQ TQFI+F +S R+RR D+FQ R+FLK ENI W+ N
Sbjct: 721 TRGSPLLESRKPFDWNLLASAFGTQITQFISFMTSVRARRSDVFQRRDFLKPENIVWYAN 780

Query: 783 NQSSPQWEDPSCKFLAVMLR--ADKEENGSITENAKPRS-DIFMAFNASDQSESVALPEP 842
           +Q++P+WEDP+ KFLA+ ++  +++EE  S+ E  +P+S D+F+ FNASD  ESV LP  
Sbjct: 781 DQTTPKWEDPASKFLALEIKSESEEEETASLAEPNEPKSNDLFIGFNASDHPESVVLPSL 840

Query: 843 SEGTSWFRLVDTALSFPGFFSFNGELV----PTTGSVTYEMQAHSCALFE 882
            +G+ W RLVDTAL FPGFFS  GE V    P    V YEM+ +SC LFE
Sbjct: 841 PDGSKWRRLVDTALPFPGFFSVEGETVVAEEPLQQLVVYEMKPYSCTLFE 876

BLAST of Cla97C09G169430 vs. TAIR 10
Match: AT1G03310.1 (debranching enzyme 1 )

HSP 1 Score: 933.3 bits (2411), Expect = 1.4e-271
Identity = 477/890 (53.60%), Postives = 628/890 (70.56%), Query Frame = 0

Query: 3   MATFPSSLVIQPFCVYNCGVTESFKLAVSDHHT-YGQKIKYQSGKMDEARMLAHGERKVS 62
           MA +  S+ I   C+ N G+T ++K   +   T    KIK  S  +   R    G+   S
Sbjct: 1   MAAWSPSVGIGSCCLNN-GITRTWKFPSARLFTGRKNKIKLGSETLMFTRKRFMGDLVTS 60

Query: 63  FMKSSHRNLSIAYATSGISVGKSGQRFSTGGKSKEHRKVATYLFRTEFGDLVNVFVEKTG 122
            ++S   +   A  T   S+       S   ++ + +KV +Y FRT+ G LV V VEK  
Sbjct: 61  ALQSYQFSKICASKT---SIELREALSSRRAEADDLKKVTSYSFRTKAGALVKVKVEKKR 120

Query: 123 SGFTVNIEISSVQRANIDET-LLLSWGVYRSDSTLVTP-NFQSSLPDETIGATETPFLKT 182
             +++ + +SS++ +  D++ L++ WGVYRSDS+   P +F++S  D     TET F+K+
Sbjct: 121 EKYSILVYVSSLELSGDDKSRLVMVWGVYRSDSSCFLPLDFENSSQDSQTHTTETTFVKS 180

Query: 183 SEGKFAVELEFDAKHTPFYLSFLLKYSLGVDSGSSEIRSHRKTSFPVPVGFGRGYPSPLG 242
           S  +  + LEFD K +PFYLSF LK   G D    E+ +HR T F +PVGF  G+P PLG
Sbjct: 181 SLSELMLGLEFDGKESPFYLSFHLKLVSGRDPDGQEMLTHRDTDFCIPVGFTAGHPLPLG 240

Query: 243 LSIS-GDGSINFSIFSSTAESVVLCLYNDSTSEKPVLELDLDPYINKSGNIWHASFEGAS 302
           LS    D S NFS FS ++ +VVLCLY+DST++KP LELDLDPY+N++G++WHAS +   
Sbjct: 241 LSSGPDDDSWNFSFFSRSSTNVVLCLYDDSTTDKPALELDLDPYVNRTGDVWHASVDNTW 300

Query: 303 KFVSYGYRCKGSKFHENQDGVEVSQIVVDPYAKILTASIPKARGQGLGLPSNLLGQICKV 362
            FV YGYRCK +   +    VE   IV+DPYA ++          G  +    LG + K 
Sbjct: 301 DFVRYGYRCKETAHSKEDVDVEGEPIVLDPYATVV----------GKSVSQKYLGSLSKS 360

Query: 363 PAFDWDGEVRPNLPMEKLFVYRLNVKHFTIDKSSQLPADIAGTFSGLTKKLLHFKNLGVN 422
           P+FDW  +V PN+P+EKL VYRLNVK FT  +SS+LP+++AGTFSG+ +K+ H K LG N
Sbjct: 361 PSFDWGEDVSPNIPLEKLLVYRLNVKGFTQHRSSKLPSNVAGTFSGVAEKVSHLKTLGTN 420

Query: 423 AVQLEPIFQFDEKEGPYFPFHFFSPTNSYGPSGASISVINSMKEMVKELHANGIEVILEV 482
           AV LEPIF F E++GPYFPFHFFSP + YGPS +  S +NSMK MVK+LH+ GIEV+LEV
Sbjct: 421 AVLLEPIFSFSEQKGPYFPFHFFSPMDIYGPSNSLESAVNSMKVMVKKLHSEGIEVLLEV 480

Query: 483 VFTHTAVDGALQGIDDSSYYFVNRVANLEGKSVLNCNYPIVQQLLLDSLRYWVTEFHIDG 542
           VFTHTA  GAL+GIDDSSYY+  R  +L+ KS LNCNYP+VQQL+L+SLRYWVTEFH+DG
Sbjct: 481 VFTHTADSGALRGIDDSSYYYKGRANDLDSKSYLNCNYPVVQQLVLESLRYWVTEFHVDG 540

Query: 543 FCFINASSLLRGHHGELLSRPPFVEAVAFDPLLSKTKLVADFWDPQDLVSKETHFPHWKR 602
           FCFINASSLLRG HGE LSRPP VEA+AFDPLL++TKL+AD WDP +++ KE  FPHWKR
Sbjct: 541 FCFINASSLLRGVHGEQLSRPPLVEAIAFDPLLAETKLIADCWDPLEMMPKEVRFPHWKR 600

Query: 603 WAEVNSKFCNDIRDFFRGEGLISSLATRLCGSGDVFSDGRGPAFSFNFIARNVGLPLVDL 662
           WAE+N+++C ++R+F RG G++S LATR+CGSGDVF+DGRGPAFSFN+I+RN GL LVD+
Sbjct: 601 WAELNTRYCRNVRNFLRGRGVLSDLATRICGSGDVFTDGRGPAFSFNYISRNSGLSLVDI 660

Query: 663 VSFSDSKLASELSSNCGEEGPTSNIEVLEKRLKQIRNFIFVLFVSLGVPVLNMGDECGQS 722
           VSFS  +LASELS NCGEEG T+   VL++RLKQIRNF+F+ ++SLGVPVLNMGDECG S
Sbjct: 661 VSFSGPELASELSWNCGEEGATNKSAVLQRRLKQIRNFLFIQYISLGVPVLNMGDECGIS 720

Query: 723 SGGSVASNDRRSFNWDLLKTDFGTQTTQFIAFFSSFRSRRFDLFQSRNFLKGENIDWFDN 782
           + GS     R+ F+W+LL + FGTQ TQFI+F +S R+RR D+FQ R+FLK ENI W+ N
Sbjct: 721 TRGSPLLESRKPFDWNLLASAFGTQITQFISFMTSVRARRSDVFQRRDFLKPENIVWYAN 780

Query: 783 NQSSPQWEDPSCKFLAVMLR--ADKEENGSITENAKPRS-DIFMAFNASDQSESVALPEP 842
           +Q++P+WEDP+ KFLA+ ++  +++EE  S+ E  +P+S D+F+ FNASD  ESV LP  
Sbjct: 781 DQTTPKWEDPASKFLALEIKSESEEEETASLAEPNEPKSNDLFIGFNASDHPESVVLPSL 840

Query: 843 SEGTSWFRLVDTALSFPGFFSFNGELV----PTTGSVTYEMQAHSCALFE 882
            +G+ W RLVDTAL FPGFFS  GE V    P    V YEM+ +SC LFE
Sbjct: 841 PDGSKWRRLVDTALPFPGFFSVEGETVVAEEPLQQLVVYEMKPYSCTLFE 876

BLAST of Cla97C09G169430 vs. TAIR 10
Match: AT4G09020.1 (isoamylase 3 )

HSP 1 Score: 367.1 bits (941), Expect = 4.0e-101
Identity = 246/710 (34.65%), Postives = 354/710 (49.86%), Query Frame = 0

Query: 233 GYPSPLGLSISGDGSINFSIFSSTAESVVLCLY-----NDSTSEKPVLELDLDPYINKSG 292
           G  SPLG+S   D  INF++FS  A SV LCL       D T +  ++EL LDP +NK+G
Sbjct: 88  GEVSPLGVS-QVDKGINFALFSQNATSVTLCLSLSQSGKDDTDDDGMIELVLDPSVNKTG 147

Query: 293 NIWHASFEGAS-KFVSYGYRCKGSKFHENQDGVEVSQIVVDPYAKILTASIPKARGQGLG 352
           + WH   E      V YGYR  G    +     + S +++DPYAK++            G
Sbjct: 148 DTWHICVEDLPLNNVLYGYRVDGPGEWQQGHRFDRSILLLDPYAKLVKGH------SSFG 207

Query: 353 LPSNLLGQI-----CKVPAFDWDGEVR-PNLPMEKLFVYRLNVKHFTIDKSSQLPADIAG 412
             S    Q       +   FDW  + + PN+P + L +Y +NV+ FT D+SS +   I G
Sbjct: 208 DSSQKFAQFYGTYDFESSPFDWGDDYKFPNIPEKDLVIYEMNVRAFTADESSGMDPAIGG 267

Query: 413 TFSGLTKKLLHFKNLGVNAVQLEPIFQFDEKE--------------GPYFPFHFFSPTNS 472
           ++ G  +K+ H ++LG+NAV+L P+F+FDE E                Y   +FF+P + 
Sbjct: 268 SYLGFIEKIPHLQDLGINAVELLPVFEFDELELQRRSNPRDHMVNTWGYSTVNFFAPMSR 327

Query: 473 YGP-SGASISVINSMKEMVKELHANGIEVILEVVFTHT-------AVDGALQGIDDSSYY 532
           Y    G  I      KEMVK LH+ GIEVIL+VV+ HT           + +GID+  YY
Sbjct: 328 YASGEGDPIKASKEFKEMVKALHSAGIEVILDVVYNHTNEADDKYPYTTSFRGIDNKVYY 387

Query: 533 FV---NRVANLEG-KSVLNCNYPIVQQLLLDSLRYWVTEFHIDGFCFINASSLLRGHHGE 592
            +   N++ N  G  + LNCN+P+V +L+LDSLR+WVTE+H+DGF F  AS L R   G 
Sbjct: 388 MLDPNNQLLNFSGCGNTLNCNHPVVMELILDSLRHWVTEYHVDGFRFDLASVLCRATDGS 447

Query: 593 LLSRPPFVEAVAFDPLLSKTKLVADFWDPQD--LVSKETHFPHWKRWAEVNSKFCNDIRD 652
            LS PP + A+A D +LS+ K++A+ WD     LV K   FP+W RWAE N  + +D+R 
Sbjct: 448 PLSAPPLIRAIAKDSVLSRCKIIAEPWDCGGLYLVGK---FPNWDRWAEWNGMYRDDVRR 507

Query: 653 FFRGE-GLISSLATRLCGSGDVFS-DGRGPAFSFNFIARNVGLPLVDLVSFS-------- 712
           F +G+ G+  S ATR+ GS D++  + R P    NF+  + G  L DLVS++        
Sbjct: 508 FIKGDSGMKGSFATRVSGSSDLYQVNQRKPYHGVNFVIAHDGFTLRDLVSYNFKHNEANG 567

Query: 713 ---DSKLASELSSNCGEEGPTSNIEVLEKRLKQIRNFIFVLFVSLGVPVLNMGDECGQSS 772
              +       S NCG EG T +  +   R +Q++NF   L +S G P++ MGDE G + 
Sbjct: 568 EGGNDGCNDNHSWNCGFEGETGDAHIKSLRTRQMKNFHLALMISQGTPMMLMGDEYGHTR 627

Query: 773 GGSVAS----NDRRSFNWDLLKTDFGTQTTQFIAFFSSFRSRRFD--LFQSRNFLKGENI 832
            G+  S        +F W     +   +      FFS     R    + +  NFL    I
Sbjct: 628 YGNNNSYGHDTSLNNFQW----KELDAKKQNHFRFFSEVIKFRHSHHVLKHENFLTQGEI 687

Query: 833 DWFDNNQSSPQWEDPSCKFLAVMLRADKEENGSITENAKPRSDIFMAFNASDQSESVALP 884
            W ++N     W++   KFLA  L            +     DI++AFNA D      +P
Sbjct: 688 TWHEDN-----WDNSESKFLAFTL-----------HDGIGGRDIYVAFNAHDYFVKALIP 747

BLAST of Cla97C09G169430 vs. TAIR 10
Match: AT2G39930.1 (isoamylase 1 )

HSP 1 Score: 341.7 bits (875), Expect = 1.8e-93
Identity = 233/690 (33.77%), Postives = 343/690 (49.71%), Query Frame = 0

Query: 233 GYPSPLGLSISGDGSINFSIFSSTAESVVLCL--YNDSTSEKPVLELDLDPYINKSGNIW 292
           G PSP G ++  DG +NFS++S+ + S  +CL   +D    K   E+ LDP  N++G++W
Sbjct: 72  GLPSPFGPTVRDDG-VNFSVYSTNSVSATICLISLSDLRQNKVTEEIQLDPSRNRTGHVW 131

Query: 293 HASFEGASKFVSYGYRCKGSKFHENQDGVEVSQIVVDPYAKILTASIPKARGQGLGLPSN 352
           H    G  K + YGYR  G    E     + S I++DPYAK   A I +     LG   N
Sbjct: 132 HVFLRGDFKDMLYGYRFDGKFSPEEGHYYDSSNILLDPYAK---AIISRDEFGVLGPDDN 191

Query: 353 LLGQI-CKVPA----FDWDGEVRPNLPMEKLFVYRLNVKHFTIDKSSQLPADIAGTFSGL 412
              Q+ C VP     FDW+G++   LP + L +Y ++V+ FT  +SS++  +  GT+ G+
Sbjct: 192 CWPQMACMVPTREEEFDWEGDMHLKLPQKDLVIYEMHVRGFTRHESSKI--EFPGTYQGV 251

Query: 413 TKKLLHFKNLGVNAVQLEPIFQFDEKE---------------GPYFPFHFFSPTNSYGPS 472
            +KL H K LG+N ++L P  +F+E E                 Y    FFSP   Y  +
Sbjct: 252 AEKLDHLKELGINCIELMPCHEFNELEYYSYNTILGDHRVNFWGYSTIGFFSPMIRYASA 311

Query: 473 GASI---SVINSMKEMVKELHANGIEVILEVVFTHTAVDG------ALQGIDDSSYYFV- 532
            ++      IN  K +VKE H  GIEVI++VV  HTA         + +G+D+S YY + 
Sbjct: 312 SSNNFAGRAINEFKILVKEAHKRGIEVIMDVVLNHTAEGNEKGPIFSFRGVDNSVYYMLA 371

Query: 533 --NRVANLEG-KSVLNCNYPIVQQLLLDSLRYWVTEFHIDGFCFINASSLLRGH------ 592
                 N  G  +  NCN+P+V+Q +LD LRYWVTE H+DGF F   S + R        
Sbjct: 372 PKGEFYNYSGCGNTFNCNHPVVRQFILDCLRYWVTEMHVDGFRFDLGSIMSRSSSLWDAA 431

Query: 593 -------HGELL------SRPPFVEAVAFDPLLSKTKLVADFWDPQDLVSKETHFPHWKR 652
                   G+LL      S PP ++ ++ DP+L   KL+A+ WD   L  +   FPHW  
Sbjct: 432 NVYGADVEGDLLTTGTPISCPPVIDMISNDPILRGVKLIAEAWDAGGLY-QVGMFPHWGI 491

Query: 653 WAEVNSKFCNDIRDFFRG-EGLISSLATRLCGSGDVFSDGRGPAFSFNFIARNVGLPLVD 712
           W+E N KF + +R F +G +G   + A  LCGS +++  GR P  S NFI  + G  L D
Sbjct: 492 WSEWNGKFRDVVRQFIKGTDGFSGAFAECLCGSPNLYQGGRKPWHSINFICAHDGFTLAD 551

Query: 713 LVSFSD-SKLAS----------ELSSNCGEEGPTSNIEVLEKRLKQIRNFIFVLFVSLGV 772
           LV++++ + LA+            S NCGEEG  ++I V   R +Q+RNF   L VS GV
Sbjct: 552 LVTYNNKNNLANGEENNDGENHNYSWNCGEEGDFASISVKRLRKRQMRNFFVSLMVSQGV 611

Query: 773 PVLNMGDECGQSSGGS----VASNDRRSFNWDLLKTDFGTQTTQFIAFFSSFRSRRFDLF 832
           P++ MGDE G + GG+       N    F WD  K +  +   +F      FR    +  
Sbjct: 612 PMIYMGDEYGHTKGGNNNTYCHDNYMNYFRWD-KKEEAHSDFFRFCRILIKFRD-ECESL 671

Query: 833 QSRNFLKGENIDWFDNNQSSPQWEDPSCKFLAVMLRADKEENGSITENAKPRSDIFMAFN 853
              +F   + + W       P W + S +F+A           S+ ++ K   +I++AFN
Sbjct: 672 GLNDFPTAKRLQWHGLAPEIPNWSETS-RFVAF----------SLVDSVK--KEIYVAFN 731

BLAST of Cla97C09G169430 vs. TAIR 10
Match: AT5G04360.1 (limit dextrinase )

HSP 1 Score: 81.6 bits (200), Expect = 3.4e-15
Identity = 94/396 (23.74%), Postives = 158/396 (39.90%), Query Frame = 0

Query: 202 LLKYSLGVDSGSSEIRSHRKTSFPVPVGFGR--GYPSPLGLSISGDGSINFSIFSSTAES 261
           L+K  L V    +E R  + T   +P        Y  PLG   + +G ++  +++ TA++
Sbjct: 170 LVKSQLAVVCFDAEGRLIQGTGLQLPGVLDELFSYDGPLGAHFTPEG-VSLHLWAPTAQA 229

Query: 262 VVLCLYNDSTSEKPVLELDLDPYINKSGNIWHASFEGASKFVSYGYRCKGSKFHENQDGV 321
           V +C+Y +   + P   +++ P + ++  +W  S EGA  +    Y  K S +H +   +
Sbjct: 230 VSVCIYKNPLDKSP---MEICP-LKEANGVW--STEGACSWGGCYYVYKVSVYHPSTMKL 289

Query: 322 EVSQIVVDPYAKILTASIPKARGQGLGLPSNLLGQICKVPAFDWDGEVRPNL-PMEKLFV 381
           E      DPYA+ L+A   K          NL     K   +D   + +P L     + +
Sbjct: 290 ETC-YANDPYARGLSADGRKT------FLVNLDSDDLKPEGWDNLADKKPCLRSFSDISI 349

Query: 382 YRLNVKHFTIDKSSQLPADIAGTFSGLTKKLLHFKNL------GVNAVQLEPIFQF---- 441
           Y L+V+ F+ +  +  P +  G  +  +K     K+L      G+  + L P FQF    
Sbjct: 350 YELHVRDFSANDETVEPENRGGYLAFTSKDSAGVKHLQKLVDAGLTHLHLLPTFQFGDVD 409

Query: 442 DEKEG----------------------------------PYFPFHFFSPTNSYGPSGASI 501
           DEKE                                    Y P  +  P  SY       
Sbjct: 410 DEKENWKSVDTSLLEGLRPDSTEAQARITEIQNDDGYNWGYNPVLWGVPKGSYASDPTGP 469

Query: 502 SVINSMKEMVKELHANGIEVILEVVFTHTAVDG------ALQGIDDSSYYFVNRVANLEG 542
             I   ++MV+ L+  G+ V+L+VV+ H    G       L  I    Y   N    +E 
Sbjct: 470 CRIIEFRKMVQALNCTGLNVVLDVVYNHLHASGPHDKESVLDKIVPGYYLRRNSDGFIEN 529

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
XP_038898155.10.0e+0091.07isoamylase 2, chloroplastic [Benincasa hispida][more]
XP_008451396.10.0e+0089.03PREDICTED: isoamylase 2, chloroplastic [Cucumis melo][more]
XP_004136188.10.0e+0088.36isoamylase 2, chloroplastic [Cucumis sativus] >KGN44865.1 hypothetical protein C... [more]
KAA0057598.10.0e+0088.91isoamylase 2 [Cucumis melo var. makuwa] >TYK20996.1 isoamylase 2 [Cucumis melo v... [more]
KAG7014422.10.0e+0086.89Isoamylase 2, chloroplastic [Cucurbita argyrosperma subsp. argyrosperma][more]
Match NameE-valueIdentityDescription
Q8L7352.0e-27053.60Isoamylase 2, chloroplastic OS=Arabidopsis thaliana OX=3702 GN=ISA2 PE=1 SV=2[more]
Q6AU805.3e-21551.96Isoamylase 2, chloroplastic OS=Oryza sativa subsp. japonica OX=39947 GN=ISA2 PE=... [more]
Q9M0S55.7e-10034.65Isoamylase 3, chloroplastic OS=Arabidopsis thaliana OX=3702 GN=ISA3 PE=1 SV=2[more]
B9G4349.7e-10034.76Isoamylase 3, chloroplastic OS=Oryza sativa subsp. japonica OX=39947 GN=ISA3 PE=... [more]
D0TZF03.9e-9333.53Isoamylase 1, chloroplastic OS=Oryza sativa subsp. japonica OX=39947 GN=ISA1 PE=... [more]
Match NameE-valueIdentityDescription
A0A1S3BRF80.0e+0089.03isoamylase 2, chloroplastic OS=Cucumis melo OX=3656 GN=LOC103492696 PE=4 SV=1[more]
A0A0A0K5510.0e+0088.36Aamy domain-containing protein OS=Cucumis sativus OX=3659 GN=Csa_7G392380 PE=4 S... [more]
A0A5A7UR470.0e+0088.91Isoamylase 2 OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold328G00230 P... [more]
A0A6J1GPU30.0e+0086.78isoamylase 2, chloroplastic isoform X1 OS=Cucurbita moschata OX=3662 GN=LOC11145... [more]
A0A6J1GN630.0e+0085.99isoamylase 2, chloroplastic isoform X2 OS=Cucurbita moschata OX=3662 GN=LOC11145... [more]
Match NameE-valueIdentityDescription
AT1G03310.21.4e-27153.60debranching enzyme 1 [more]
AT1G03310.11.4e-27153.60debranching enzyme 1 [more]
AT4G09020.14.0e-10134.65isoamylase 3 [more]
AT2G39930.11.8e-9333.77isoamylase 1 [more]
AT5G04360.13.4e-1523.74limit dextrinase [more]
InterPro
Analysis Name: InterPro Annotations of Watermelon (97103) v2.5
Date Performed: 2022-01-31
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR006047Glycosyl hydrolase, family 13, catalytic domainSMARTSM00642aamycoord: 375..755
e-value: 0.001
score: -73.7
IPR006047Glycosyl hydrolase, family 13, catalytic domainPFAMPF00128Alpha-amylasecoord: 400..487
e-value: 4.2E-9
score: 36.4
NoneNo IPR availableGENE3D3.20.20.80Glycosidasescoord: 357..770
e-value: 6.6E-100
score: 337.1
NoneNo IPR availablePANTHERPTHR43002GLYCOGEN DEBRANCHING ENZYMEcoord: 73..885
NoneNo IPR availablePANTHERPTHR43002:SF6ISOAMYLASE 2, CHLOROPLASTICcoord: 73..885
NoneNo IPR availableSUPERFAMILY51011Glycosyl hydrolase domaincoord: 771..883
IPR013780Glycosyl hydrolase, all-betaGENE3D2.60.40.1180coord: 771..887
e-value: 3.9E-25
score: 90.2
IPR013783Immunoglobulin-like foldGENE3D2.60.40.10Immunoglobulinscoord: 226..343
e-value: 6.4E-28
score: 99.0
IPR004193Glycoside hydrolase, family 13, N-terminalPFAMPF02922CBM_48coord: 237..330
e-value: 1.3E-13
score: 51.1
IPR044505Glycogen debranching enzyme GlgX/isoamylase, N-terminal Early set domainCDDcd02856E_set_GDE_Isoamylase_Ncoord: 236..380
e-value: 4.61921E-26
score: 101.954
IPR044096Isoamylase 2, catalytic domainCDDcd11346AmyAc_plant_IsoAcoord: 372..763
e-value: 1.96947E-142
score: 424.192
IPR014756Immunoglobulin E-setSUPERFAMILY81296E set domainscoord: 233..368
IPR017853Glycoside hydrolase superfamilySUPERFAMILY51445(Trans)glycosidasescoord: 357..750

Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
Cla97C09G169430.2Cla97C09G169430.2mRNA


GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
biological_process GO:0005977 glycogen metabolic process
biological_process GO:0019252 starch biosynthetic process
biological_process GO:0005975 carbohydrate metabolic process
molecular_function GO:0019156 isoamylase activity
molecular_function GO:0003824 catalytic activity
molecular_function GO:0004553 hydrolase activity, hydrolyzing O-glycosyl compounds