Cla97C09G168930 (gene) Watermelon (97103) v2.5

Overview
NameCla97C09G168930
Typegene
OrganismCitrullus lanatus subsp. vulgaris cv. 97103 (Watermelon (97103) v2.5)
DescriptionE3 ubiquitin-protein ligase MIEL1-like
LocationCla97Chr09: 5256274 .. 5256600 (+)
RNA-Seq ExpressionCla97C09G168930
SyntenyCla97C09G168930
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: exonCDSpolypeptide
Hold the cursor over a type above to highlight its positions in the sequence below.
ATGGCTGCCTTCAGATCCCTACTTTCCATAGCTCTCTTGGCTCTTCTCTTCATCTTCTCCGAATCAATACCCACTTCCAATTCTCTTACAGGAATCCCTCGCCGGGACGTCTTTCAGACGGCGGCTCATTGGATTACAAAAAGCAGTGTTATTGAGTCCTCTTCCCTAAGGAAATCCAATAATTTCCATATCGAAGCTAGAAGGCGGATTCGACCTGTACTAATCGGCAGCAACCATCCTAAATCGGCCGCAAATTGGAGCCGAGTAGCATCCTTTGAGGTTGGATCTGTGTTGTCTATTTGCATTTTATTGGGATTGTTCTTCTGA

mRNA sequence

ATGGCTGCCTTCAGATCCCTACTTTCCATAGCTCTCTTGGCTCTTCTCTTCATCTTCTCCGAATCAATACCCACTTCCAATTCTCTTACAGGAATCCCTCGCCGGGACGTCTTTCAGACGGCGGCTCATTGGATTACAAAAAGCAGTGTTATTGAGTCCTCTTCCCTAAGGAAATCCAATAATTTCCATATCGAAGCTAGAAGGCGGATTCGACCTGTACTAATCGGCAGCAACCATCCTAAATCGGCCGCAAATTGGAGCCGAGTAGCATCCTTTGAGGTTGGATCTGTGTTGTCTATTTGCATTTTATTGGGATTGTTCTTCTGA

Coding sequence (CDS)

ATGGCTGCCTTCAGATCCCTACTTTCCATAGCTCTCTTGGCTCTTCTCTTCATCTTCTCCGAATCAATACCCACTTCCAATTCTCTTACAGGAATCCCTCGCCGGGACGTCTTTCAGACGGCGGCTCATTGGATTACAAAAAGCAGTGTTATTGAGTCCTCTTCCCTAAGGAAATCCAATAATTTCCATATCGAAGCTAGAAGGCGGATTCGACCTGTACTAATCGGCAGCAACCATCCTAAATCGGCCGCAAATTGGAGCCGAGTAGCATCCTTTGAGGTTGGATCTGTGTTGTCTATTTGCATTTTATTGGGATTGTTCTTCTGA

Protein sequence

MAAFRSLLSIALLALLFIFSESIPTSNSLTGIPRRDVFQTAAHWITKSSVIESSSLRKSNNFHIEARRRIRPVLIGSNHPKSAANWSRVASFEVGSVLSICILLGLFF
Homology
BLAST of Cla97C09G168930 vs. NCBI nr
Match: KAA0046715.1 (E3 ubiquitin-protein ligase MIEL1-like [Cucumis melo var. makuwa] >TYK18251.1 E3 ubiquitin-protein ligase MIEL1-like [Cucumis melo var. makuwa])

HSP 1 Score: 117.1 bits (292), Expect = 9.5e-23
Identity = 75/110 (68.18%), Postives = 83/110 (75.45%), Query Frame = 0

Query: 1   MAAFRSLLSIALLALLFIFSESIPTSNSLTGIPRRDVFQTAAHWITKSSVIESSSLRKSN 60
           MAA R L+SIALLALLF FSES+ TS+SLTGIP R++  T AH I   SV ESS  RKSN
Sbjct: 1   MAAIRPLVSIALLALLF-FSESLLTSHSLTGIPHRNILHTVAHRIVNGSV-ESSYQRKSN 60

Query: 61  NFHIEARRRIRPVLI-GSN--HPKSAANWSRVASFEVGSVLSICILLGLF 108
             H + RRRIRP+LI GSN  H KSA N SRVASFEVGS+L ICI LGLF
Sbjct: 61  KLHTKDRRRIRPILISGSNGMHHKSAENRSRVASFEVGSMLCICIFLGLF 108

BLAST of Cla97C09G168930 vs. NCBI nr
Match: XP_008451651.1 (PREDICTED: E3 ubiquitin-protein ligase MIEL1-like [Cucumis melo])

HSP 1 Score: 97.8 bits (242), Expect = 6.0e-17
Identity = 64/96 (66.67%), Postives = 71/96 (73.96%), Query Frame = 0

Query: 1  MAAFRSLLSIALLALLFIFSESIPTSNSLTGIPRRDVFQTAAHWITKSSVIESSSLRKSN 60
          MAA R L+SIALLALLF FSES+ TS+SLTGIP R++  T AH I   SV ESS  RKSN
Sbjct: 1  MAAIRPLVSIALLALLF-FSESLLTSHSLTGIPHRNILHTVAHRIVNGSV-ESSYQRKSN 60

Query: 61 NFHIEARRRIRPVLI-GSN--HPKSAANWSRVASFE 94
            H + RRRIRP+LI GSN  H KSA N SRVASFE
Sbjct: 61 KLHTKDRRRIRPILISGSNGMHHKSAENRSRVASFE 94

BLAST of Cla97C09G168930 vs. NCBI nr
Match: KAG6575859.1 (hypothetical protein SDJN03_26498, partial [Cucurbita argyrosperma subsp. sororia] >KAG7014393.1 hypothetical protein SDJN02_24570, partial [Cucurbita argyrosperma subsp. argyrosperma])

HSP 1 Score: 80.9 bits (198), Expect = 7.6e-12
Identity = 62/115 (53.91%), Postives = 77/115 (66.96%), Query Frame = 0

Query: 1   MAAFRSLLSIALLALLFIFSESIPT--------SNSLTGIPRRDVFQTAAHWITKSSVIE 60
           MAA +SL+S+ALLALL +FSES+          SNSLTG+ RR+V QTAAH I  +SV E
Sbjct: 1   MAALKSLVSVALLALL-LFSESLTASAVDRSGDSNSLTGMLRRNVLQTAAHQIADNSV-E 60

Query: 61  SSSLRKSNNFHIEARRRIRPVLIGSNHPKSAANWSRVASFEVGSVLSICILLGLF 108
           SS  RK N   +  +RR+RPV  G+    SAAN SR+AS  VGSVL   ++LGLF
Sbjct: 61  SSPERKMN---VHIKRRMRPVATGTR--SSAANRSRIASIGVGSVL---VVLGLF 105

BLAST of Cla97C09G168930 vs. NCBI nr
Match: KAG6593163.1 (hypothetical protein SDJN03_12639, partial [Cucurbita argyrosperma subsp. sororia])

HSP 1 Score: 71.6 bits (174), Expect = 4.6e-09
Identity = 58/116 (50.00%), Postives = 69/116 (59.48%), Query Frame = 0

Query: 1   MAAFRSLLSIALLALLFIFSESIPT--------SNSLTGIPRRDVFQTAAHWITKSSVIE 60
           MAA ++LLSIAL+ALL +F ES+PT        S SL GI RR++ QT A          
Sbjct: 1   MAAIKALLSIALIALL-LFFESLPTSAAVHRGASTSLAGILRRNIVQTTAD--------H 60

Query: 61  SSSLRKSNNFHIEARRRIRPVLIGSNHPK-SAANWSRVASFEVGSVLSICILLGLF 108
                K  N HI+ R R+RPVLIG   PK SAAN SR A   VGS+L I +LLGLF
Sbjct: 61  HQVAEKKFNVHIKRRMRMRPVLIG--RPKTSAANRSREAFCGVGSMLCIFVLLGLF 105

BLAST of Cla97C09G168930 vs. NCBI nr
Match: XP_022992473.1 (uncharacterized protein LOC111488793 isoform X1 [Cucurbita maxima])

HSP 1 Score: 60.5 bits (145), Expect = 1.1e-05
Identity = 45/116 (38.79%), Postives = 64/116 (55.17%), Query Frame = 0

Query: 1   MAAFRSLLSIALLALLFIFSESIPTS--------NSLTGIPRRDVFQTAAHWITKSSVIE 60
           MA  +SL+SIALLALL +F E +P S        + L G+ R D  QTA     +   + 
Sbjct: 63  MAPIKSLISIALLALLLLF-ERLPISAADHGVAPSPLAGVFRLDTLQTA-----ERHQVA 122

Query: 61  SSSLRKSNNFHIEARRRIRPVLIGSNHPK-SAANWSRVASFEVGSVLSICILLGLF 108
            + +    +F  + RRR R  +  +  P  S+ANWSR +SF VGS+L +C + GLF
Sbjct: 123 ENGIGNQYSFEQKIRRRARRAVRRAEGPSPSSANWSRTSSFGVGSLLCLCTVFGLF 172

BLAST of Cla97C09G168930 vs. ExPASy TrEMBL
Match: A0A5D3D3Y6 (E3 ubiquitin-protein ligase MIEL1-like OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold411G001920 PE=4 SV=1)

HSP 1 Score: 117.1 bits (292), Expect = 4.6e-23
Identity = 75/110 (68.18%), Postives = 83/110 (75.45%), Query Frame = 0

Query: 1   MAAFRSLLSIALLALLFIFSESIPTSNSLTGIPRRDVFQTAAHWITKSSVIESSSLRKSN 60
           MAA R L+SIALLALLF FSES+ TS+SLTGIP R++  T AH I   SV ESS  RKSN
Sbjct: 1   MAAIRPLVSIALLALLF-FSESLLTSHSLTGIPHRNILHTVAHRIVNGSV-ESSYQRKSN 60

Query: 61  NFHIEARRRIRPVLI-GSN--HPKSAANWSRVASFEVGSVLSICILLGLF 108
             H + RRRIRP+LI GSN  H KSA N SRVASFEVGS+L ICI LGLF
Sbjct: 61  KLHTKDRRRIRPILISGSNGMHHKSAENRSRVASFEVGSMLCICIFLGLF 108

BLAST of Cla97C09G168930 vs. ExPASy TrEMBL
Match: A0A0A0K929 (Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_7G394020 PE=4 SV=1)

HSP 1 Score: 99.4 bits (246), Expect = 9.9e-18
Identity = 69/114 (60.53%), Postives = 78/114 (68.42%), Query Frame = 0

Query: 1   MAAFRSLLSIALLALLFIFSESIPTSNSLTGIPRRDVFQTAAHWITKSSVIESS-SLRKS 60
           MAA R LLSIA LA LF FSES+ TS+SLTGIP R +  TAAH I   SV  SS  +RKS
Sbjct: 1   MAAIRPLLSIAFLA-LFFFSESLLTSHSLTGIPHRTILNTAAHRIVDGSVEYSSYQIRKS 60

Query: 61  NNFHIE--ARRRIRPVLIGS---NHPKSAANW-SRVASFEVGSVLSICILLGLF 108
           N  HI+   +RR  P+L+ S   NH KSAAN  S VAS EVGS+L ICI LGLF
Sbjct: 61  NKLHIKDIRKRRSGPILVSSSNGNHHKSAANRPSPVASLEVGSMLCICIFLGLF 113

BLAST of Cla97C09G168930 vs. ExPASy TrEMBL
Match: A0A1S3BT53 (E3 ubiquitin-protein ligase MIEL1-like OS=Cucumis melo OX=3656 GN=LOC103492883 PE=4 SV=1)

HSP 1 Score: 97.8 bits (242), Expect = 2.9e-17
Identity = 64/96 (66.67%), Postives = 71/96 (73.96%), Query Frame = 0

Query: 1  MAAFRSLLSIALLALLFIFSESIPTSNSLTGIPRRDVFQTAAHWITKSSVIESSSLRKSN 60
          MAA R L+SIALLALLF FSES+ TS+SLTGIP R++  T AH I   SV ESS  RKSN
Sbjct: 1  MAAIRPLVSIALLALLF-FSESLLTSHSLTGIPHRNILHTVAHRIVNGSV-ESSYQRKSN 60

Query: 61 NFHIEARRRIRPVLI-GSN--HPKSAANWSRVASFE 94
            H + RRRIRP+LI GSN  H KSA N SRVASFE
Sbjct: 61 KLHTKDRRRIRPILISGSNGMHHKSAENRSRVASFE 94

BLAST of Cla97C09G168930 vs. ExPASy TrEMBL
Match: A0A5A7VLE5 (Protein no-on-transient A-like OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold411G00010 PE=4 SV=1)

HSP 1 Score: 60.5 bits (145), Expect = 5.1e-06
Identity = 52/111 (46.85%), Postives = 65/111 (58.56%), Query Frame = 0

Query: 1   MAAFRSLLSIALLALLFIFSESIPT--------SNSLTGIPRRDVFQTAAHWITKSSVIE 60
           MAA +SLL I+LLALLF FSES+ T        SNS+TG+ RR+V QTA H I  S    
Sbjct: 1   MAAIKSLLFISLLALLF-FSESLSTSAADCRGASNSVTGMLRRNVLQTATHEIADS---- 60

Query: 61  SSSLRKSNNFHIE--ARRRIRPVLIG---SNHPKSAANWSRVASFEVGSVL 99
                   N HI+     RIRPV  G   + + KSAA+ S+VASF +G +L
Sbjct: 61  ----EGKMNVHIKRGGGGRIRPVAAGTGFTGNRKSAASRSQVASFGLGFLL 102

BLAST of Cla97C09G168930 vs. ExPASy TrEMBL
Match: A0A6J1JZB1 (uncharacterized protein LOC111488793 isoform X1 OS=Cucurbita maxima OX=3661 GN=LOC111488793 PE=4 SV=1)

HSP 1 Score: 60.5 bits (145), Expect = 5.1e-06
Identity = 45/116 (38.79%), Postives = 64/116 (55.17%), Query Frame = 0

Query: 1   MAAFRSLLSIALLALLFIFSESIPTS--------NSLTGIPRRDVFQTAAHWITKSSVIE 60
           MA  +SL+SIALLALL +F E +P S        + L G+ R D  QTA     +   + 
Sbjct: 63  MAPIKSLISIALLALLLLF-ERLPISAADHGVAPSPLAGVFRLDTLQTA-----ERHQVA 122

Query: 61  SSSLRKSNNFHIEARRRIRPVLIGSNHPK-SAANWSRVASFEVGSVLSICILLGLF 108
            + +    +F  + RRR R  +  +  P  S+ANWSR +SF VGS+L +C + GLF
Sbjct: 123 ENGIGNQYSFEQKIRRRARRAVRRAEGPSPSSANWSRTSSFGVGSLLCLCTVFGLF 172

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
KAA0046715.19.5e-2368.18E3 ubiquitin-protein ligase MIEL1-like [Cucumis melo var. makuwa] >TYK18251.1 E3... [more]
XP_008451651.16.0e-1766.67PREDICTED: E3 ubiquitin-protein ligase MIEL1-like [Cucumis melo][more]
KAG6575859.17.6e-1253.91hypothetical protein SDJN03_26498, partial [Cucurbita argyrosperma subsp. sorori... [more]
KAG6593163.14.6e-0950.00hypothetical protein SDJN03_12639, partial [Cucurbita argyrosperma subsp. sorori... [more]
XP_022992473.11.1e-0538.79uncharacterized protein LOC111488793 isoform X1 [Cucurbita maxima][more]
Match NameE-valueIdentityDescription
Match NameE-valueIdentityDescription
A0A5D3D3Y64.6e-2368.18E3 ubiquitin-protein ligase MIEL1-like OS=Cucumis melo var. makuwa OX=1194695 GN... [more]
A0A0A0K9299.9e-1860.53Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_7G394020 PE=4 SV=1[more]
A0A1S3BT532.9e-1766.67E3 ubiquitin-protein ligase MIEL1-like OS=Cucumis melo OX=3656 GN=LOC103492883 P... [more]
A0A5A7VLE55.1e-0646.85Protein no-on-transient A-like OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_s... [more]
A0A6J1JZB15.1e-0638.79uncharacterized protein LOC111488793 isoform X1 OS=Cucurbita maxima OX=3661 GN=L... [more]
Match NameE-valueIdentityDescription
Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
Cla97C09G168930.2Cla97C09G168930.2mRNA


GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
molecular_function GO:0046872 metal ion binding