Cla97C09G166850 (gene) Watermelon (97103) v2.5

Overview
NameCla97C09G166850
Typegene
OrganismCitrullus lanatus subsp. vulgaris cv. 97103 (Watermelon (97103) v2.5)
DescriptionCytochrome P450 protein
LocationCla97Chr09: 3786613 .. 3788999 (+)
RNA-Seq ExpressionCla97C09G166850
SyntenyCla97C09G166850
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: exonCDSpolypeptide
Hold the cursor over a type above to highlight its positions in the sequence below.
ATGGTTTCTGCTGTCTTATATTGCATGACTATTCCACTTATCCTCACCTTTTTCCAACAAAAAACTTCCTCCATGTTTTCCCATGCATTTCCATTCCTTCATCACAAACCTATGATGGATTCCGGCAACCTCTCCTCCTCCTCCCCCACCGCGGCCTTGCCTCCTCCAATTTCTGTTGGAAACGGGCTCCGGTTGCTCCTCGATGTCCAAATCTTGGAAATCTCTTTCGCTCTTTTTGTGTTCTTAGTCATCCATGTCATTAGACAGAAGAGACGATATGGCTTGCCTATATGGCCAATCGTTGGAATGTTACCGGCTTTGATTACGGCAGCACGTGGGAATCTCTACGACTGGATTACTGACCTCCTCCGCCATCACAAGGGCACGTTTCAATTTCGAGGCCCCTGGCTTACTAGTCTCAATTGTGTCGTCACTGCTGATCCTCGAAACTTGGAGCACCTTCTCAAGACCAAATTTTCCTCCTACCCAAAAGGGCCATTCTTTCGTGACACGGTGAGAGACCTACTCGGTGACGGAATATTCGGTGCCGACGATGAGACGTGGCAACGTCAAAGGAAGGTGGCGAGCATTGAGTTTCACTCGGCGAGGTTCCGACAAATGACGGTGGAGTCGTTGGTGGAGCTCGTCCACTTGAGGTTAGAAGCGGTGTTGGAGGATTTCGCGGGACGGTCGGCAACGGTGGACCTGCAGGATGTTCTTCTGAGGTTAACGTTTGACAATGTGTGTATGATTGCCTTTGGGGTGGATCCTGGCTGTTTGCGCGGCGGCTTACCGGAGATTCCATTTGCTAAGGCCTTTGAGGATGCCACTGAAGTTACTGTTATACGCTTCATCACACCTACTTTCGTATGGAAAGCCATGAGGTATTTTCCCTTTCTAATTTTTTAATTGAATTTTTAAATACTAAATATTTTGTTTAAAAATAGTTTATGATAGAGCTGCCAACCAATAACTAACTAAAATAGTTAAGATATTCCTAGTAATTAAGATGAAATGTTCATATAATTTTTACTCCCTGCTTCTATAGTAATAAAAATATTTTGGTTAAATTATGTGTTTAAATAGTAAATTTGGTTGGTATGATTTAATGATAGTGACAATTTAATTTTTATGATTTATGATTATAATTTAATCTCTCCCATGGATAATATTTATGAGAATTTTATGAAATTATAGTGATTGAATTAAATTCTAATTTTTTCCCGAACCAAATTTTGTAATCTTGAGAGGTGTTTGGAGAAAAAAAAACTGTTGTTTAGTTTTGAGACAATAAAGATAAATGAATTTTTTTAATATCATATGTCCAATTTCTTAAATATATAAACATATATCAAATCATTTTTCAGACATTATACTAAGAAAAACTCTATAATCAAACTTAATTAAATAATTTAATTTTAAGCAGGTACCTGAACTTGGGTATGGAAAAGAAACTGAAGCAATCGATCAAAGCCGTCGACGAATTTGCAGATAATGTGATTCGAACACGGAAGAGAGAGCTCTCTACGGCGGCGCAACCGCAAGCCGATAACCAAAGATCTGACCTATTAACCGTATTCATGAAGCTAAGAGACGAGCAAGGGCGGCCGTTCTCCGACAAGTTTCTCAGAGATATTTGCGTCAATTTCATACTTGCCGGAAGAGACACATCTTCCGTAGCATTGAGTTGGTTCTTCTGGTTGCTCGATCAAAATCCCCATGTGGAAGACAAAATTCTGGCAGAAATTGGCAGAATCGTCGGCGAACGAGAAGAAGCCAAAACCAGAGAGCTTCGATTCGATTTGTTAGTGTTCAAACCAGAGGAAATCAAGAAAATGGAGTATTTGCACGCCGCCATTTCCGAAGCCCTAAGATTGTATCCATCAGTTCCAGTCGATCACAAAGAGGTAATTAATAATTACTCATTCACATTCCTATTTCGAAAGCAAATCTCAGAAATCTAATAAATTATCCGTCCGATTCAGGTCGTGGAGGACGATGTATTCCCAGACGGAACGATTCTGAAGAAAGGAACGAAAATCATCTACGCAATATACGCAATGGGAAGAATGGAAGCGATTTGGGGGAAAGATTGTCGCGAATTCAAACCTGAGAGATGGATGAGAGACGGTCGGTTCATGAGCGAATCGGCGTATCGGTTCACAGCCTTCAACGGCGGCCCTCGGCTGTGTCTTGGAAAGGATTTTGCGTACTATCAAATGAAATACGTCGCCGCTTCGATCATGAGCCGGTACCGGGTGGCGGTGGTGACGGGCCATTCAGTGGAGCCGAAGCTTGCCCTAACTTTCTACATGAAAAACGGCCTGAAAGTCAATTTGATTAAACGAGAAGAGGGGCAGATTCGGAAATACATGAAGTTAAATTGA

mRNA sequence

ATGGTTTCTGCTGTCTTATATTGCATGACTATTCCACTTATCCTCACCTTTTTCCAACAAAAAACTTCCTCCATGTTTTCCCATGCATTTCCATTCCTTCATCACAAACCTATGATGGATTCCGGCAACCTCTCCTCCTCCTCCCCCACCGCGGCCTTGCCTCCTCCAATTTCTGTTGGAAACGGGCTCCGGTTGCTCCTCGATGTCCAAATCTTGGAAATCTCTTTCGCTCTTTTTGTGTTCTTAGTCATCCATGTCATTAGACAGAAGAGACGATATGGCTTGCCTATATGGCCAATCGTTGGAATGTTACCGGCTTTGATTACGGCAGCACGTGGGAATCTCTACGACTGGATTACTGACCTCCTCCGCCATCACAAGGGCACGTTTCAATTTCGAGGCCCCTGGCTTACTAGTCTCAATTGTGTCGTCACTGCTGATCCTCGAAACTTGGAGCACCTTCTCAAGACCAAATTTTCCTCCTACCCAAAAGGGCCATTCTTTCGTGACACGGTGAGAGACCTACTCGGTGACGGAATATTCGGTGCCGACGATGAGACGTGGCAACGTCAAAGGAAGGTGGCGAGCATTGAGTTTCACTCGGCGAGGTTCCGACAAATGACGGTGGAGTCGTTGGTGGAGCTCGTCCACTTGAGGTTAGAAGCGGTGTTGGAGGATTTCGCGGGACGGTCGGCAACGGTGGACCTGCAGGATGTTCTTCTGAGGTTAACGTTTGACAATGTGTGTATGATTGCCTTTGGGGTGGATCCTGGCTGTTTGCGCGGCGGCTTACCGGAGATTCCATTTGCTAAGGCCTTTGAGGATGCCACTGAAGTTACTGTTATACGCTTCATCACACCTACTTTCGTATGGAAAGCCATGAGGTACCTGAACTTGGGTATGGAAAAGAAACTGAAGCAATCGATCAAAGCCGTCGACGAATTTGCAGATAATGTGATTCGAACACGGAAGAGAGAGCTCTCTACGGCGGCGCAACCGCAAGCCGATAACCAAAGATCTGACCTATTAACCGTATTCATGAAGCTAAGAGACGAGCAAGGGCGGCCGTTCTCCGACAAGTTTCTCAGAGATATTTGCGTCAATTTCATACTTGCCGGAAGAGACACATCTTCCGTAGCATTGAGTTGGTTCTTCTGGTTGCTCGATCAAAATCCCCATGTGGAAGACAAAATTCTGGCAGAAATTGGCAGAATCGTCGGCGAACGAGAAGAAGCCAAAACCAGAGAGCTTCGATTCGATTTGTTAGTGTTCAAACCAGAGGAAATCAAGAAAATGGAGTATTTGCACGCCGCCATTTCCGAAGCCCTAAGATTGTATCCATCAGTTCCAGTCGATCACAAAGAGGTCGTGGAGGACGATGTATTCCCAGACGGAACGATTCTGAAGAAAGGAACGAAAATCATCTACGCAATATACGCAATGGGAAGAATGGAAGCGATTTGGGGGAAAGATTGTCGCGAATTCAAACCTGAGAGATGGATGAGAGACGGTCGGTTCATGAGCGAATCGGCGTATCGGTTCACAGCCTTCAACGGCGGCCCTCGGCTGTGTCTTGGAAAGGATTTTGCGTACTATCAAATGAAATACGTCGCCGCTTCGATCATGAGCCGGTACCGGGTGGCGGTGGTGACGGGCCATTCAGTGGAGCCGAAGCTTGCCCTAACTTTCTACATGAAAAACGGCCTGAAAGTCAATTTGATTAAACGAGAAGAGGGGCAGATTCGGAAATACATGAAGTTAAATTGA

Coding sequence (CDS)

ATGGTTTCTGCTGTCTTATATTGCATGACTATTCCACTTATCCTCACCTTTTTCCAACAAAAAACTTCCTCCATGTTTTCCCATGCATTTCCATTCCTTCATCACAAACCTATGATGGATTCCGGCAACCTCTCCTCCTCCTCCCCCACCGCGGCCTTGCCTCCTCCAATTTCTGTTGGAAACGGGCTCCGGTTGCTCCTCGATGTCCAAATCTTGGAAATCTCTTTCGCTCTTTTTGTGTTCTTAGTCATCCATGTCATTAGACAGAAGAGACGATATGGCTTGCCTATATGGCCAATCGTTGGAATGTTACCGGCTTTGATTACGGCAGCACGTGGGAATCTCTACGACTGGATTACTGACCTCCTCCGCCATCACAAGGGCACGTTTCAATTTCGAGGCCCCTGGCTTACTAGTCTCAATTGTGTCGTCACTGCTGATCCTCGAAACTTGGAGCACCTTCTCAAGACCAAATTTTCCTCCTACCCAAAAGGGCCATTCTTTCGTGACACGGTGAGAGACCTACTCGGTGACGGAATATTCGGTGCCGACGATGAGACGTGGCAACGTCAAAGGAAGGTGGCGAGCATTGAGTTTCACTCGGCGAGGTTCCGACAAATGACGGTGGAGTCGTTGGTGGAGCTCGTCCACTTGAGGTTAGAAGCGGTGTTGGAGGATTTCGCGGGACGGTCGGCAACGGTGGACCTGCAGGATGTTCTTCTGAGGTTAACGTTTGACAATGTGTGTATGATTGCCTTTGGGGTGGATCCTGGCTGTTTGCGCGGCGGCTTACCGGAGATTCCATTTGCTAAGGCCTTTGAGGATGCCACTGAAGTTACTGTTATACGCTTCATCACACCTACTTTCGTATGGAAAGCCATGAGGTACCTGAACTTGGGTATGGAAAAGAAACTGAAGCAATCGATCAAAGCCGTCGACGAATTTGCAGATAATGTGATTCGAACACGGAAGAGAGAGCTCTCTACGGCGGCGCAACCGCAAGCCGATAACCAAAGATCTGACCTATTAACCGTATTCATGAAGCTAAGAGACGAGCAAGGGCGGCCGTTCTCCGACAAGTTTCTCAGAGATATTTGCGTCAATTTCATACTTGCCGGAAGAGACACATCTTCCGTAGCATTGAGTTGGTTCTTCTGGTTGCTCGATCAAAATCCCCATGTGGAAGACAAAATTCTGGCAGAAATTGGCAGAATCGTCGGCGAACGAGAAGAAGCCAAAACCAGAGAGCTTCGATTCGATTTGTTAGTGTTCAAACCAGAGGAAATCAAGAAAATGGAGTATTTGCACGCCGCCATTTCCGAAGCCCTAAGATTGTATCCATCAGTTCCAGTCGATCACAAAGAGGTCGTGGAGGACGATGTATTCCCAGACGGAACGATTCTGAAGAAAGGAACGAAAATCATCTACGCAATATACGCAATGGGAAGAATGGAAGCGATTTGGGGGAAAGATTGTCGCGAATTCAAACCTGAGAGATGGATGAGAGACGGTCGGTTCATGAGCGAATCGGCGTATCGGTTCACAGCCTTCAACGGCGGCCCTCGGCTGTGTCTTGGAAAGGATTTTGCGTACTATCAAATGAAATACGTCGCCGCTTCGATCATGAGCCGGTACCGGGTGGCGGTGGTGACGGGCCATTCAGTGGAGCCGAAGCTTGCCCTAACTTTCTACATGAAAAACGGCCTGAAAGTCAATTTGATTAAACGAGAAGAGGGGCAGATTCGGAAATACATGAAGTTAAATTGA

Protein sequence

MVSAVLYCMTIPLILTFFQQKTSSMFSHAFPFLHHKPMMDSGNLSSSSPTAALPPPISVGNGLRLLLDVQILEISFALFVFLVIHVIRQKRRYGLPIWPIVGMLPALITAARGNLYDWITDLLRHHKGTFQFRGPWLTSLNCVVTADPRNLEHLLKTKFSSYPKGPFFRDTVRDLLGDGIFGADDETWQRQRKVASIEFHSARFRQMTVESLVELVHLRLEAVLEDFAGRSATVDLQDVLLRLTFDNVCMIAFGVDPGCLRGGLPEIPFAKAFEDATEVTVIRFITPTFVWKAMRYLNLGMEKKLKQSIKAVDEFADNVIRTRKRELSTAAQPQADNQRSDLLTVFMKLRDEQGRPFSDKFLRDICVNFILAGRDTSSVALSWFFWLLDQNPHVEDKILAEIGRIVGEREEAKTRELRFDLLVFKPEEIKKMEYLHAAISEALRLYPSVPVDHKEVVEDDVFPDGTILKKGTKIIYAIYAMGRMEAIWGKDCREFKPERWMRDGRFMSESAYRFTAFNGGPRLCLGKDFAYYQMKYVAASIMSRYRVAVVTGHSVEPKLALTFYMKNGLKVNLIKREEGQIRKYMKLN
Homology
BLAST of Cla97C09G166850 vs. NCBI nr
Match: XP_008461361.1 (PREDICTED: LOW QUALITY PROTEIN: cytochrome P450 86B1-like [Cucumis melo])

HSP 1 Score: 1073.2 bits (2774), Expect = 9.0e-310
Identity = 537/589 (91.17%), Postives = 558/589 (94.74%), Query Frame = 0

Query: 1   MVSAVLYCMTIPLILTFFQQKTSSMFSHAFPFLHHKPMMDSGNLSSSSPTAALPPPISVG 60
           M+ A LYCMTI LIL FF  KTSSMFSHAFPFLHHKP+MDSGNLSSSS +++LPP ISV 
Sbjct: 1   MLFAALYCMTIQLILHFFTTKTSSMFSHAFPFLHHKPIMDSGNLSSSS-SSSLPPSISVR 60

Query: 61  N-GLRLLLDVQILEISFALFVFLVIHVIRQKRRYGLPIWPIVGMLPALITAARGNLYDWI 120
           + GL+ LLDVQILEISFALFVFLVIHV+RQKRR+GLPIWPIVGMLPALITA RGNLY+WI
Sbjct: 61  HFGLQSLLDVQILEISFALFVFLVIHVVRQKRRHGLPIWPIVGMLPALITATRGNLYEWI 120

Query: 121 TDLLRHHKGTFQFRGPWLTSLNCVVTADPRNLEHLLKTKFSSYPKGPFFRDTVRDLLGDG 180
           TDLL  HKGTFQFRGPWLTSLNCVVTADPRNLEHLLKTKFSSYPKG FFRDTVRDLLGDG
Sbjct: 121 TDLLCRHKGTFQFRGPWLTSLNCVVTADPRNLEHLLKTKFSSYPKGSFFRDTVRDLLGDG 180

Query: 181 IFGADDETWQRQRKVASIEFHSARFRQMTVESLVELVHLRLEAVLEDFAGRSATVDLQDV 240
           IFGADDETWQRQRKVASIEFHSARFRQMTVESLVEL H RLEAVLEDFAG+S TVDLQDV
Sbjct: 181 IFGADDETWQRQRKVASIEFHSARFRQMTVESLVELAHSRLEAVLEDFAGKSTTVDLQDV 240

Query: 241 LLRLTFDNVCMIAFGVDPGCLRGGLPEIPFAKAFEDATEVTVIRFITPTFVWKAMRYLNL 300
           LLRLTFDNVCMIAFGVDPGCLRGGLPEIPFAKAFEDATEVTV+RFITPTF+WKAMRYLN+
Sbjct: 241 LLRLTFDNVCMIAFGVDPGCLRGGLPEIPFAKAFEDATEVTVMRFITPTFIWKAMRYLNI 300

Query: 301 GMEKKLKQSIKAVDEFADNVIRTRKRELSTAAQPQADNQRSDLLTVFMKLRDEQGRPFSD 360
           G+EKKLKQSIKAVDEFA+NVI+TRKRELST A+ QADN+RSDLLTVFMKLRDEQGRPFSD
Sbjct: 301 GIEKKLKQSIKAVDEFAENVIQTRKRELSTPAESQADNKRSDLLTVFMKLRDEQGRPFSD 360

Query: 361 KFLRDICVNFILAGRDTSSVALSWFFWLLDQNPHVEDKILAEIGRIVGEREEAKTRELRF 420
           KFLRDICVNFILAGRDTSSVALSWFFWLLDQNP VEDKILAEI RIVGEREEAKTRELRF
Sbjct: 361 KFLRDICVNFILAGRDTSSVALSWFFWLLDQNPDVEDKILAEICRIVGEREEAKTRELRF 420

Query: 421 DLLVFKPEEIKKMEYLHAAISEALRLYPSVPVDHKEVVEDDVFPDGTILKKGTKIIYAIY 480
           D LVFKPEE KKMEYLHAAISEALRLYPSVP DHKEVVEDD FPDGTILKKG KIIYAIY
Sbjct: 421 DSLVFKPEEXKKMEYLHAAISEALRLYPSVPFDHKEVVEDDTFPDGTILKKGMKIIYAIY 480

Query: 481 AMGRMEAIWGKDCREFKPERWMRDGRFMSESAYRFTAFNGGPRLCLGKDFAYYQMKYVAA 540
           AMGRMEAIWGKDCREFKPERWMRDGRFMSES YRFTAFNGGPRLCLGKDFAYYQMKYVAA
Sbjct: 481 AMGRMEAIWGKDCREFKPERWMRDGRFMSESVYRFTAFNGGPRLCLGKDFAYYQMKYVAA 540

Query: 541 SIMSRYRVAVVTGHSVEPKLALTFYMKNGLKVNLIKREEGQIRKYMKLN 589
           SI+SRYRVAVV GH VEPKL+LTFYMK GLKVNLIKREEGQIRKYMKLN
Sbjct: 541 SIISRYRVAVVAGHPVEPKLSLTFYMKKGLKVNLIKREEGQIRKYMKLN 588

BLAST of Cla97C09G166850 vs. NCBI nr
Match: XP_004135954.3 (cytochrome P450 86B1 [Cucumis sativus] >KAE8646384.1 hypothetical protein Csa_015511 [Cucumis sativus])

HSP 1 Score: 1063.9 bits (2750), Expect = 4.9e-307
Identity = 530/589 (89.98%), Postives = 557/589 (94.57%), Query Frame = 0

Query: 1   MVSAVLYCMTIPLILTFFQQKTSSMFSHAFPFLHHKPMMDSGNLSSSSPTAALPPPISVG 60
           M+ A LYCMTI LIL FF   TSSMFSHAFPFLHHKP+MDSGNLSSSS +++  PPIS+G
Sbjct: 1   MLFAALYCMTIQLILHFFTTITSSMFSHAFPFLHHKPIMDSGNLSSSSSSSSFSPPISIG 60

Query: 61  N-GLRLLLDVQILEISFALFVFLVIHVIRQKRRYGLPIWPIVGMLPALITAARGNLYDWI 120
           + GLR LLDVQILEI+FALFVFLVIHV+RQKRR+GLPIWPIVGMLPALITA RGNLY+WI
Sbjct: 61  HFGLRSLLDVQILEITFALFVFLVIHVVRQKRRHGLPIWPIVGMLPALITATRGNLYEWI 120

Query: 121 TDLLRHHKGTFQFRGPWLTSLNCVVTADPRNLEHLLKTKFSSYPKGPFFRDTVRDLLGDG 180
           TDLL  HKGTFQFRGPWLTSLNCVVTADPRNLEHLLKTKFSSYPKG FFRDTVRDLLGDG
Sbjct: 121 TDLLCRHKGTFQFRGPWLTSLNCVVTADPRNLEHLLKTKFSSYPKGSFFRDTVRDLLGDG 180

Query: 181 IFGADDETWQRQRKVASIEFHSARFRQMTVESLVELVHLRLEAVLEDFAGRSATVDLQDV 240
           IFGADDETWQRQRKVASIEFHSARFRQMTVESLVELVH RLEAVLEDF G++ TVDLQDV
Sbjct: 181 IFGADDETWQRQRKVASIEFHSARFRQMTVESLVELVHSRLEAVLEDFEGQATTVDLQDV 240

Query: 241 LLRLTFDNVCMIAFGVDPGCLRGGLPEIPFAKAFEDATEVTVIRFITPTFVWKAMRYLNL 300
           LLRLTFDNVCMIAFGVDPGCLR GLPEIPFAKAFEDATEVTVIRFITPTF+WK MRYLN+
Sbjct: 241 LLRLTFDNVCMIAFGVDPGCLRSGLPEIPFAKAFEDATEVTVIRFITPTFIWKFMRYLNI 300

Query: 301 GMEKKLKQSIKAVDEFADNVIRTRKRELSTAAQPQADNQRSDLLTVFMKLRDEQGRPFSD 360
           G+EKKLKQSIKAVDEFA++VI+TRKRELST A+ QA+NQRSDLLTVFMKLRDEQGRPFSD
Sbjct: 301 GIEKKLKQSIKAVDEFAEDVIQTRKRELSTPAESQAENQRSDLLTVFMKLRDEQGRPFSD 360

Query: 361 KFLRDICVNFILAGRDTSSVALSWFFWLLDQNPHVEDKILAEIGRIVGEREEAKTRELRF 420
           KFLRDICVNFILAGRDTSSVALSWFF LLDQNP VEDKIL EI RIVGEREEAKTRELRF
Sbjct: 361 KFLRDICVNFILAGRDTSSVALSWFFLLLDQNPDVEDKILEEICRIVGEREEAKTRELRF 420

Query: 421 DLLVFKPEEIKKMEYLHAAISEALRLYPSVPVDHKEVVEDDVFPDGTILKKGTKIIYAIY 480
           D LVFKPEEIK+MEYLHAAISEALRLYPSVP DHKEVVEDDVFPDGT+LKKGTKIIYAIY
Sbjct: 421 DSLVFKPEEIKRMEYLHAAISEALRLYPSVPFDHKEVVEDDVFPDGTVLKKGTKIIYAIY 480

Query: 481 AMGRMEAIWGKDCREFKPERWMRDGRFMSESAYRFTAFNGGPRLCLGKDFAYYQMKYVAA 540
           AMGRMEAIWGKDCREFKPERWMR+GRFMSESAYRFTAFNGGPRLCLGKDFAYYQMKYVAA
Sbjct: 481 AMGRMEAIWGKDCREFKPERWMREGRFMSESAYRFTAFNGGPRLCLGKDFAYYQMKYVAA 540

Query: 541 SIMSRYRVAVVTGHSVEPKLALTFYMKNGLKVNLIKREEGQIRKYMKLN 589
           SI+SRYRVAVV GH VEPKLALTFYMK GLKVNLIKREEGQ+RKY+KLN
Sbjct: 541 SIISRYRVAVVRGHPVEPKLALTFYMKKGLKVNLIKREEGQLRKYIKLN 589

BLAST of Cla97C09G166850 vs. NCBI nr
Match: XP_022954102.1 (cytochrome P450 86B1-like [Cucurbita moschata])

HSP 1 Score: 1036.6 bits (2679), Expect = 8.4e-299
Identity = 514/565 (90.97%), Postives = 536/565 (94.87%), Query Frame = 0

Query: 25  MFSHAFPFLHHKPMMDSGNLSSSSPTAALPPPISVGN-GLRLLLDVQILEISFALFVFLV 84
           MFSHA PFLHHKPMMDSGNLSSSS   ALP  +S GN GL  LLD QILEISFALFVFL 
Sbjct: 1   MFSHAIPFLHHKPMMDSGNLSSSS---ALPALVSAGNFGLWPLLDFQILEISFALFVFLA 60

Query: 85  IHVIRQKRRYGLPIWPIVGMLPALITAARGNLYDWITDLLRHHKGTFQFRGPWLTSLNCV 144
           IHV+RQ+RRYGLP+WPIVGMLP+LITAARGNLY+WITDLLRHHKGTFQFRGPW +SLNCV
Sbjct: 61  IHVVRQQRRYGLPVWPIVGMLPSLITAARGNLYEWITDLLRHHKGTFQFRGPWFSSLNCV 120

Query: 145 VTADPRNLEHLLKTKFSSYPKGPFFRDTVRDLLGDGIFGADDETWQRQRKVASIEFHSAR 204
           VTADPRNLEHLLKTKF SYPKGPFFRDTVRDLLGDGIFGADDETWQRQRKVASIEFHSAR
Sbjct: 121 VTADPRNLEHLLKTKFPSYPKGPFFRDTVRDLLGDGIFGADDETWQRQRKVASIEFHSAR 180

Query: 205 FRQMTVESLVELVHLRLEAVLEDFAGRSATVDLQDVLLRLTFDNVCMIAFGVDPGCLRGG 264
           FRQMTVESLVELVH RLE VLEDF GR   VDLQDVLLRLTFDNVCMIAFGVDPGCLRGG
Sbjct: 181 FRQMTVESLVELVHSRLETVLEDFLGRRTAVDLQDVLLRLTFDNVCMIAFGVDPGCLRGG 240

Query: 265 LPEIPFAKAFEDATEVTVIRFITPTFVWKAMRYLNLGMEKKLKQSIKAVDEFADNVIRTR 324
           LPEIPFAKAFEDATEVTV+RFITPTFVWK +RYLNLG+EKKLKQSI+AVDEFA++VI+TR
Sbjct: 241 LPEIPFAKAFEDATEVTVLRFITPTFVWKILRYLNLGVEKKLKQSIQAVDEFAEDVIQTR 300

Query: 325 KRELSTAAQPQADNQRSDLLTVFMKLRDEQGRPFSDKFLRDICVNFILAGRDTSSVALSW 384
           KRELST AQPQA+NQRSDLLTVFM+LRDEQ RPFSDKFLRDICVNFILAGRDTSSVALSW
Sbjct: 301 KRELSTTAQPQAENQRSDLLTVFMRLRDEQRRPFSDKFLRDICVNFILAGRDTSSVALSW 360

Query: 385 FFWLLDQNPHVEDKILAEIGRIVGEREEAKTRELRFDLLVFKPEEIKKMEYLHAAISEAL 444
           FFWLLD+NP VEDKILAEI RIVGEREEAKTRE RFD LVFKPEEIKKMEYLHAAISEAL
Sbjct: 361 FFWLLDRNPDVEDKILAEIYRIVGEREEAKTREFRFDSLVFKPEEIKKMEYLHAAISEAL 420

Query: 445 RLYPSVPVDHKEVVEDDVFPDGTILKKGTKIIYAIYAMGRMEAIWGKDCREFKPERWMRD 504
           RLYPSVPVDHKEVVEDDVFPDGTILKKGTKIIYAIYAMGRMEAIWGKDCREFKPERWM+D
Sbjct: 421 RLYPSVPVDHKEVVEDDVFPDGTILKKGTKIIYAIYAMGRMEAIWGKDCREFKPERWMKD 480

Query: 505 GRFMSESAYRFTAFNGGPRLCLGKDFAYYQMKYVAASIMSRYRVAVVTGHSVEPKLALTF 564
           GRFMSESAYRFTAFNGGPRLCLG+DFAYYQMKYVAASI+SRYRVAVV GH VEPKLALTF
Sbjct: 481 GRFMSESAYRFTAFNGGPRLCLGRDFAYYQMKYVAASIISRYRVAVVKGHPVEPKLALTF 540

Query: 565 YMKNGLKVNLIKREEGQIRKYMKLN 589
           YMK+GLKVNLIKREEGQIRKY+KLN
Sbjct: 541 YMKDGLKVNLIKREEGQIRKYVKLN 562

BLAST of Cla97C09G166850 vs. NCBI nr
Match: XP_023549182.1 (cytochrome P450 86B1-like [Cucurbita pepo subsp. pepo])

HSP 1 Score: 1036.6 bits (2679), Expect = 8.4e-299
Identity = 512/565 (90.62%), Postives = 534/565 (94.51%), Query Frame = 0

Query: 25  MFSHAFPFLHHKPMMDSGNLSSSSPTAALPPPISVGN-GLRLLLDVQILEISFALFVFLV 84
           MFSHA PFLHHKPMMDSGNLSSSS ++ALP  +S G  GL  LLDVQILEISFALFVF  
Sbjct: 1   MFSHAIPFLHHKPMMDSGNLSSSSSSSALPALVSDGKFGLWPLLDVQILEISFALFVFFA 60

Query: 85  IHVIRQKRRYGLPIWPIVGMLPALITAARGNLYDWITDLLRHHKGTFQFRGPWLTSLNCV 144
           IHV+RQ+RRYGLP+WPIVGMLP+LITAARGNLY+WITDLLR HKGTFQFRGPW +SLNCV
Sbjct: 61  IHVVRQRRRYGLPVWPIVGMLPSLITAARGNLYEWITDLLRRHKGTFQFRGPWFSSLNCV 120

Query: 145 VTADPRNLEHLLKTKFSSYPKGPFFRDTVRDLLGDGIFGADDETWQRQRKVASIEFHSAR 204
           VTADPRNLEHLLKTKF SYPKGPFFRDTVRDLLGDGIFGADDETWQRQRKVASIEFHSAR
Sbjct: 121 VTADPRNLEHLLKTKFPSYPKGPFFRDTVRDLLGDGIFGADDETWQRQRKVASIEFHSAR 180

Query: 205 FRQMTVESLVELVHLRLEAVLEDFAGRSATVDLQDVLLRLTFDNVCMIAFGVDPGCLRGG 264
           FRQMTVESLVELVHLRLE VLEDF GR   VDLQDVLLRLTFDNVCMIAFGVDPGCL GG
Sbjct: 181 FRQMTVESLVELVHLRLETVLEDFVGRRTAVDLQDVLLRLTFDNVCMIAFGVDPGCLSGG 240

Query: 265 LPEIPFAKAFEDATEVTVIRFITPTFVWKAMRYLNLGMEKKLKQSIKAVDEFADNVIRTR 324
           LPEIPFAKAFEDATEVTV+RFITPTFVWK +RYLNLG+EK+LKQSI+AVDEFA+NVI+TR
Sbjct: 241 LPEIPFAKAFEDATEVTVLRFITPTFVWKILRYLNLGVEKRLKQSIQAVDEFAENVIQTR 300

Query: 325 KRELSTAAQPQADNQRSDLLTVFMKLRDEQGRPFSDKFLRDICVNFILAGRDTSSVALSW 384
           KRELST AQPQADNQRSDLLTVFM+LRDEQGR FSDKFLRDICVNFILAGRDTSSVALSW
Sbjct: 301 KRELSTTAQPQADNQRSDLLTVFMRLRDEQGRSFSDKFLRDICVNFILAGRDTSSVALSW 360

Query: 385 FFWLLDQNPHVEDKILAEIGRIVGEREEAKTRELRFDLLVFKPEEIKKMEYLHAAISEAL 444
           FFWLLD+NP VEDKILAEI RIVGEREEAKTRE RFD LVFKPEEIKKMEYLHAAISEAL
Sbjct: 361 FFWLLDRNPDVEDKILAEIYRIVGEREEAKTREFRFDSLVFKPEEIKKMEYLHAAISEAL 420

Query: 445 RLYPSVPVDHKEVVEDDVFPDGTILKKGTKIIYAIYAMGRMEAIWGKDCREFKPERWMRD 504
           RLYPSVPVDHKEVVEDDVFPDGTILKKGTKIIYAIYAMGRMEAIWGKDCREFKPERWM+D
Sbjct: 421 RLYPSVPVDHKEVVEDDVFPDGTILKKGTKIIYAIYAMGRMEAIWGKDCREFKPERWMKD 480

Query: 505 GRFMSESAYRFTAFNGGPRLCLGKDFAYYQMKYVAASIMSRYRVAVVTGHSVEPKLALTF 564
           GRFMSESAYRFTAFNGGPRLCLG+DFAYYQMKYVAASI+SRYRVAVV GH VEPKLALTF
Sbjct: 481 GRFMSESAYRFTAFNGGPRLCLGRDFAYYQMKYVAASIISRYRVAVVKGHPVEPKLALTF 540

Query: 565 YMKNGLKVNLIKREEGQIRKYMKLN 589
           YMK+GLKVNLIKR EGQIRKY KLN
Sbjct: 541 YMKDGLKVNLIKRNEGQIRKYTKLN 565

BLAST of Cla97C09G166850 vs. NCBI nr
Match: XP_038899430.1 (cytochrome P450 86B1-like [Benincasa hispida])

HSP 1 Score: 1035.4 bits (2676), Expect = 1.9e-298
Identity = 517/565 (91.50%), Postives = 535/565 (94.69%), Query Frame = 0

Query: 25  MFSHAFPFLHHKPMMDSGNLSSSSPTAALPPPISVGN-GLRLLLDVQILEISFALFVFLV 84
           MFSHA PFLHHKPMMDSGNLSSSS  + LPPPISV N  LR LLDVQILEISFALFVFLV
Sbjct: 1   MFSHACPFLHHKPMMDSGNLSSSS--SPLPPPISVANFRLRPLLDVQILEISFALFVFLV 60

Query: 85  IHVIRQKRRYGLPIWPIVGMLPALITAARGNLYDWITDLLRHHKGTFQFRGPWLTSLNCV 144
           IHV+RQKRRY LP+WP+VGMLPALITAARGN+Y+WIT+LL  HKGTFQFRGPWL+SL+CV
Sbjct: 61  IHVVRQKRRYDLPVWPLVGMLPALITAARGNIYEWITELLHRHKGTFQFRGPWLSSLHCV 120

Query: 145 VTADPRNLEHLLKTKFSSYPKGPFFRDTVRDLLGDGIFGADDETWQRQRKVASIEFHSAR 204
           VTADPRNLEHLLKTKFSSYPKG FFRDTVRDLLGDGIFGADDETWQRQRKVASIEFHSAR
Sbjct: 121 VTADPRNLEHLLKTKFSSYPKGSFFRDTVRDLLGDGIFGADDETWQRQRKVASIEFHSAR 180

Query: 205 FRQMTVESLVELVHLRLEAVLEDFAGRSATVDLQDVLLRLTFDNVCMIAFGVDPGCLRGG 264
           FRQMTV+SLVELVH RLE VLE+FAGRS  VDLQDVLLRLTFDNVCMIAFGVDPGCLRGG
Sbjct: 181 FRQMTVDSLVELVHSRLEPVLENFAGRSEVVDLQDVLLRLTFDNVCMIAFGVDPGCLRGG 240

Query: 265 LPEIPFAKAFEDATEVTVIRFITPTFVWKAMRYLNLGMEKKLKQSIKAVDEFADNVIRTR 324
           LPEIPFAKAFEDATEVTV+RFITPTF+WK MRYLNLGMEKKLKQSIKAVDEFA+NVI+TR
Sbjct: 241 LPEIPFAKAFEDATEVTVLRFITPTFIWKVMRYLNLGMEKKLKQSIKAVDEFAENVIQTR 300

Query: 325 KRELSTAAQPQADNQRSDLLTVFMKLRDEQGRPFSDKFLRDICVNFILAGRDTSSVALSW 384
           KRE  TAAQPQAD+QRSDLLTVFM+LRDEQGRPFSDKFLRDICVNFILAGRDTSSVALSW
Sbjct: 301 KREFYTAAQPQADDQRSDLLTVFMRLRDEQGRPFSDKFLRDICVNFILAGRDTSSVALSW 360

Query: 385 FFWLLDQNPHVEDKILAEIGRIVGEREEAKTRELRFDLLVFKPEEIKKMEYLHAAISEAL 444
           FFWLLDQNP VE KIL EI RIV EREEAKTRELR D LVFKPEEIKKMEYLHAAISEAL
Sbjct: 361 FFWLLDQNPDVEHKILEEICRIVSEREEAKTRELRLDSLVFKPEEIKKMEYLHAAISEAL 420

Query: 445 RLYPSVPVDHKEVVEDDVFPDGTILKKGTKIIYAIYAMGRMEAIWGKDCREFKPERWMRD 504
           RLYPSVPVDHKEVVEDDVFPDGTILKKGTKIIYAIYAMGRMEAIWGKDC EFKPERWMRD
Sbjct: 421 RLYPSVPVDHKEVVEDDVFPDGTILKKGTKIIYAIYAMGRMEAIWGKDCCEFKPERWMRD 480

Query: 505 GRFMSESAYRFTAFNGGPRLCLGKDFAYYQMKYVAASIMSRYRVAVVTGHSVEPKLALTF 564
           GRFMSESAYRFTAFNGGPRLCLGKDFAYYQMKYVAAS +SRYRVAVVTGH VEPKLALTF
Sbjct: 481 GRFMSESAYRFTAFNGGPRLCLGKDFAYYQMKYVAASTISRYRVAVVTGHPVEPKLALTF 540

Query: 565 YMKNGLKVNLIKREEGQIRKYMKLN 589
           YMKNGLKVNLIKREEGQI KYMKLN
Sbjct: 541 YMKNGLKVNLIKREEGQIWKYMKLN 563

BLAST of Cla97C09G166850 vs. ExPASy Swiss-Prot
Match: Q9FMY1 (Cytochrome P450 86B1 OS=Arabidopsis thaliana OX=3702 GN=CYP86B1 PE=2 SV=1)

HSP 1 Score: 761.1 bits (1964), Expect = 9.0e-219
Identity = 376/540 (69.63%), Postives = 435/540 (80.56%), Query Frame = 0

Query: 45  SSSSPTAALPPPISVGNGLRLLLDVQILEISFALFVFLVIHVIRQKRRYGLPIWPIVGML 104
           SSSS +  L     V   L LL DVQILE+  A+FVF+ IH +RQK+  GLP+WP +GML
Sbjct: 17  SSSSSSDPL-----VSRRLFLLRDVQILELLIAIFVFVAIHALRQKKYQGLPVWPFLGML 76

Query: 105 PALITAARGNLYDWITDLLRHHKGTFQFRGPWLTSLNCVVTADPRNLEHLLKTKFSSYPK 164
           P+L    RGN+Y+W++D+L    GTFQFRGPW +SLN  +T DPRN+EHLLK +FS +PK
Sbjct: 77  PSLAFGLRGNIYEWLSDVLCLQNGTFQFRGPWFSSLNSTITCDPRNVEHLLKNRFSVFPK 136

Query: 165 GPFFRDTVRDLLGDGIFGADDETWQRQRKVASIEFHSARFRQMTVESLVELVHLRLEAVL 224
           G +FRD +RDLLGDGIF ADDETWQRQRK ASIEFHSA+FRQ+T +SL ELVH RL  VL
Sbjct: 137 GSYFRDNLRDLLGDGIFNADDETWQRQRKTASIEFHSAKFRQLTTQSLFELVHKRLLPVL 196

Query: 225 EDFAGRSATVDLQDVLLRLTFDNVCMIAFGVDPGCLRGGLPEIPFAKAFEDATEVTVIRF 284
           E     S+ +DLQDVLLRLTFDNVCMIAFGVDPGCL    P IPFAKAFEDATE  V+RF
Sbjct: 197 ETSVKSSSPIDLQDVLLRLTFDNVCMIAFGVDPGCLGPDQPVIPFAKAFEDATEAAVVRF 256

Query: 285 ITPTFVWKAMRYLNLGMEKKLKQSIKAVDEFADNVIRTRKRELSTAAQPQADNQRSDLLT 344
           + PT VWK MRYL++G EKKLK+SIK VD+FAD VIRTRK+ELS   +     +RSDLLT
Sbjct: 257 VMPTCVWKFMRYLDIGTEKKLKESIKGVDDFADEVIRTRKKELSLEGE---TTKRSDLLT 316

Query: 345 VFMKLRDEQGRPFSDKFLRDICVNFILAGRDTSSVALSWFFWLLDQNPHVEDKILAEIGR 404
           VFM LRDE+G  FSDKFLRDICVNFILAGRDTSSVALSWFFWLL++NP VE+KI+ E+ +
Sbjct: 317 VFMGLRDEKGESFSDKFLRDICVNFILAGRDTSSVALSWFFWLLEKNPEVEEKIMVEMCK 376

Query: 405 IVGEREEAKTRELRFDLLVFKPEEIKKMEYLHAAISEALRLYPSVPVDHKEVVEDDVFPD 464
           I+ +R++    E      VF PEEIKKM+YL AA+SEALRLYPSVPVDHKEV EDDVFPD
Sbjct: 377 ILRQRDDHGNAEKSDYEPVFGPEEIKKMDYLQAALSEALRLYPSVPVDHKEVQEDDVFPD 436

Query: 465 GTILKKGTKIIYAIYAMGRMEAIWGKDCREFKPERWMRDGRFMSESAYRFTAFNGGPRLC 524
           GT+LKKG K+IYAIYAMGRMEAIWGKDC EF+PERW+RDGRFMSESAY+FTAFNGGPRLC
Sbjct: 437 GTMLKKGDKVIYAIYAMGRMEAIWGKDCLEFRPERWLRDGRFMSESAYKFTAFNGGPRLC 496

Query: 525 LGKDFAYYQMKYVAASIMSRYRVAVVTGHSVEPKLALTFYMKNGLKVNLIKREEGQIRKY 584
           LGKDFAYYQMK  AA+I+ RY+V VV GH VEPKLALT YMK+GL VNLI R   +I +Y
Sbjct: 497 LGKDFAYYQMKSTAAAIVYRYKVKVVNGHKVEPKLALTMYMKHGLMVNLINRSVSEIDQY 548

BLAST of Cla97C09G166850 vs. ExPASy Swiss-Prot
Match: Q9CAD6 (Cytochrome P450 86A7 OS=Arabidopsis thaliana OX=3702 GN=CYP86A7 PE=2 SV=1)

HSP 1 Score: 471.1 bits (1211), Expect = 1.9e-131
Identity = 247/517 (47.78%), Postives = 337/517 (65.18%), Query Frame = 0

Query: 67  LDVQILEISFALFVFLVIHVIRQKRRY-GLPIWPIVGMLPALITAARGNLYDWITDLLRH 126
           +D     I   L V  +I  +  +R Y G  +WP+VG LPALIT A   ++D+I D LR 
Sbjct: 1   MDGSTAAIILTLIVTYIIWFVSLRRSYKGPRVWPLVGSLPALITNAH-RMHDFIADNLRM 60

Query: 127 HKGTFQ---FRGPWLTSL--NCVVTADPRNLEHLLKTKFSSYPKGPFFRDTVRDLLGDGI 186
             GT+Q   F  P+L     +  VT DP+NLEH+LKT+F +YPKGP ++    DLLGDGI
Sbjct: 61  CGGTYQTCIFPIPFLAKKQGHVTVTCDPKNLEHILKTRFDNYPKGPSWQSVFHDLLGDGI 120

Query: 187 FGADDETWQRQRKVASIEFHSARFRQMTVESLVELVHLRLEAVLEDFAGRSATVDLQDVL 246
           F +D +TW+ QRK A++EF +   RQ     +   +  RL  +LE    R+  +DLQDVL
Sbjct: 121 FNSDGDTWRFQRKTAALEFTTRTLRQAMARWVDRAIKNRLVPILESARSRAEPIDLQDVL 180

Query: 247 LRLTFDNVCMIAFGVDPGCLRGGLPEIPFAKAFEDATEVTVIRFITPTFVWKAMRYLNLG 306
           LRLTFDN+C + FG DP  L    PE  FA AF+ ATE T+ RFI P F+WK  ++L LG
Sbjct: 181 LRLTFDNICGLTFGKDPRTLSPEFPENGFAVAFDGATEATLQRFIMPEFIWKIRKWLRLG 240

Query: 307 MEKKLKQSIKAVDEFADNVIRTRKRELSTAAQPQADNQRSDLLTVFMKLRDEQGRPFSDK 366
           +E  + +SI  VD +   +I TRK EL    Q Q +++  DLL+ FMK ++     +SDK
Sbjct: 241 LEDDMSRSISHVDNYLSEIINTRKLEL--LGQQQDESRHDDLLSRFMKKKES----YSDK 300

Query: 367 FLRDICVNFILAGRDTSSVALSWFFWLLDQNPHVEDKILAEIGRIVGEREEAKTRELRFD 426
           +L+ + +NFILAGRDTSSVA+SWFFWL+  NP VE+KI+ EI  I+ +  +    +   +
Sbjct: 301 YLKYVALNFILAGRDTSSVAMSWFFWLVSLNPRVEEKIINEICTILIKTRDTNVSKWTDE 360

Query: 427 LLVFKPEEIKKMEYLHAAISEALRLYPSVPVDHKEVVEDDVFPDGTILKKGTKIIYAIYA 486
            L F  +EI ++ YL AA+SE LRLYPSVP D K VV +DV PDGT +  G+ + Y+IY+
Sbjct: 361 PLTF--DEIDQLVYLKAALSETLRLYPSVPEDSKFVVANDVLPDGTFVPSGSNVTYSIYS 420

Query: 487 MGRMEAIWGKDCREFKPERWMRDGRFMSESAYRFTAFNGGPRLCLGKDFAYYQMKYVAAS 546
           +GRM+ IWG+DC EFKPERW+ + R    + Y+F AFN GPR+CLGKD AY QMK + AS
Sbjct: 421 VGRMKFIWGEDCLEFKPERWLEESRDEKCNQYKFVAFNAGPRICLGKDLAYLQMKSITAS 480

Query: 547 IMSRYRVAVVTGHSVEPKLALTFYMKNGLKVNLIKRE 578
           I+ R+R+ V  GH VE K++LT +MK GLK+++ KR+
Sbjct: 481 ILLRHRLTVAPGHRVEQKMSLTLFMKFGLKMDVHKRD 508

BLAST of Cla97C09G166850 vs. ExPASy Swiss-Prot
Match: B3RFJ6 (Cytochrome P450 86A22 OS=Petunia hybrida OX=4102 GN=CYP86A22 PE=1 SV=1)

HSP 1 Score: 459.9 bits (1182), Expect = 4.3e-128
Identity = 246/491 (50.10%), Postives = 321/491 (65.38%), Query Frame = 0

Query: 94  GLPIWPIVGMLPALITAARGN-LYDWITDLLRHHKGTFQ---FRGPWLTSLNCV-VTADP 153
           G  +WP+VG LP LI    GN +++WI D LR   GT+Q      P+L     V VT DP
Sbjct: 32  GPTMWPVVGSLPGLI--ENGNRMHEWIADNLRACSGTYQTCICAIPFLARKQLVTVTCDP 91

Query: 154 RNLEHLLKTKFSSYPKGPFFRDTVRDLLGDGIFGADDETWQRQRKVASIEFHSARFRQMT 213
           +NLEH+LK +F +YPKGP ++    DLLG+GIF +D +TW  QRK A++EF +   RQ  
Sbjct: 92  KNLEHILKVRFDNYPKGPTWQAVFHDLLGEGIFNSDGDTWLFQRKTAALEFTTRTLRQAM 151

Query: 214 VESLVELVHLRLEAVLEDFAGRSATVDLQDVLLRLTFDNVCMIAFGVDPGCLRGGLPEIP 273
              +   +  R   +LE    +   VDLQD+LLRLTFDN+C +AFG DP  L   LPE  
Sbjct: 152 GRWVNRAIKNRFCPILEMAQVQGKPVDLQDLLLRLTFDNICGLAFGKDPETLSPELPENN 211

Query: 274 FAKAFEDATEVTVIRFITPTFVWKAMRYLNLGMEKKLKQSIKAVDEFADNVIRTRKRELS 333
           FA +F+ ATE T+ RFI P FVWK  + L LG+E  L  S+K VD +  +VI TRK EL 
Sbjct: 212 FATSFDRATEATLHRFIMPEFVWKLKKMLGLGLEVSLNNSLKQVDNYMTDVINTRKLELL 271

Query: 334 TAAQPQADNQRSDLLTVFMKLRDEQGRPFSDKFLRDICVNFILAGRDTSSVALSWFFWLL 393
                    Q  DLL+ FMK ++     +SDKFL+ + +NFILAGRDTSSVALSWFFWL+
Sbjct: 272 N--HQNGGPQHDDLLSRFMKKKES----YSDKFLQHVALNFILAGRDTSSVALSWFFWLV 331

Query: 394 DQNPHVEDKILAEIGRIVGEREEAKTRELRFDLLVFKPEEIKKMEYLHAAISEALRLYPS 453
             NP VE+KIL EI  I+ E     T +   + LVF  EE+ ++ YL AA+SE LRLYPS
Sbjct: 332 SSNPRVEEKILVEICTILAETRGNDTSKWLEEPLVF--EEVDQLMYLKAALSETLRLYPS 391

Query: 454 VPVDHKEVVEDDVFPDGTILKKGTKIIYAIYAMGRMEAIWGKDCREFKPERWM-RDG-RF 513
           VP D K V+ DD  PDGT +  G+ I Y+IY+ GRM+ IWG+DC EFKPERWM +DG +F
Sbjct: 392 VPEDSKHVISDDYLPDGTFVPAGSNITYSIYSTGRMKFIWGEDCLEFKPERWMSQDGDKF 451

Query: 514 MSESAYRFTAFNGGPRLCLGKDFAYYQMKYVAASIMSRYRVAVVTGHSVEPKLALTFYMK 573
             +  +RF AFN GPR+CLGKD AY QMK +AA+++ R+R+AV  GH VE K++LT +MK
Sbjct: 452 QVQDTFRFVAFNAGPRICLGKDLAYLQMKSIAAAVLLRHRLAVAPGHKVEQKMSLTLFMK 511

Query: 574 NGLKVNLIKRE 578
           +GL +N+  R+
Sbjct: 512 DGLVMNVTPRD 512

BLAST of Cla97C09G166850 vs. ExPASy Swiss-Prot
Match: Q9LMM1 (Cytochrome P450 86A4 OS=Arabidopsis thaliana OX=3702 GN=CYP86A4 PE=1 SV=1)

HSP 1 Score: 459.1 bits (1180), Expect = 7.3e-128
Identity = 242/497 (48.69%), Postives = 321/497 (64.59%), Query Frame = 0

Query: 94  GLPIWPIVGMLPALITAARGNLYDWITDLLRHHKGTFQ---FRGPWLTSLN--CVVTADP 153
           G  +WP++G LP LI   R  +++WIT+ LR   GT+Q   F  P+L        VT DP
Sbjct: 29  GPRVWPLLGSLPGLI-EQRDRMHEWITENLRACGGTYQTCIFAVPFLAKKQGLVTVTCDP 88

Query: 154 RNLEHLLKTKFSSYPKGPFFRDTVRDLLGDGIFGADDETWQRQRKVASIEFHSARFRQMT 213
           +NLEH+LKT+F +YPKGP ++    DLLG GIF +D +TW  QRK A++EF +   RQ  
Sbjct: 89  KNLEHMLKTRFDNYPKGPTWQSVFHDLLGQGIFNSDGDTWLFQRKTAALEFTTRTLRQAM 148

Query: 214 VESLVELVHLRLEAVLEDFAGRSATVDLQDVLLRLTFDNVCMIAFGVDPGCLRGGLPEIP 273
              +   + LR   +L      +  VDLQD++LRLTFDN+C +AFG D      GLPE  
Sbjct: 149 GRWVNRGIKLRFCPILATAQDNAEPVDLQDLILRLTFDNICGLAFGKDTRTCAPGLPENG 208

Query: 274 FAKAFEDATEVTVIRFITPTFVWKAMRYLNLGMEKKLKQSIKAVDEFADNVIRTRKRELS 333
           FA AF+ ATE ++ RFI P F+WK  ++L LG+E  L +S+  +DE+   VI TRK+EL 
Sbjct: 209 FASAFDRATEASLQRFIIPKFMWKLKKWLGLGLEVSLSRSLGEIDEYLAAVINTRKQELM 268

Query: 334 TAAQPQADNQRSDLLTVFMKLRDEQGRPFSDKFLRDICVNFILAGRDTSSVALSWFFWLL 393
           +  +     +  DLL+ FM  + E    +SD FL+ + +NFILAGRDTSSVALSWFFWL+
Sbjct: 269 SQQESGTHQRHDDLLSRFMMKKTES---YSDTFLQHVALNFILAGRDTSSVALSWFFWLI 328

Query: 394 DQNPHVEDKILAEIGRIV----GEREEAKTRE--LRFDLLVFKPEEIKKMEYLHAAISEA 453
             +P VEDKI+ EI  ++    G  + A   E  L FD       EI ++ YL AAISE 
Sbjct: 329 TMHPTVEDKIVREICSVLIETRGTDDVASWTEEPLGFD-------EIDRLVYLKAAISET 388

Query: 454 LRLYPSVPVDHKEVVEDDVFPDGTILKKGTKIIYAIYAMGRMEAIWGKDCREFKPERWMR 513
           LRLYPSVP D K V  DDV PDGT +  G+ + Y+IYA GRM++ WG+DC EF PERW+ 
Sbjct: 389 LRLYPSVPEDSKHVENDDVLPDGTFVPAGSSVTYSIYAAGRMKSTWGEDCLEFNPERWIS 448

Query: 514 --DGRFMSESAYRFTAFNGGPRLCLGKDFAYYQMKYVAASIMSRYRVAVVTGHSVEPKLA 573
             DG+F++   YRF AFN GPR+CLGKD AY QMK +AA+++ R+R+ VV GH VE K++
Sbjct: 449 PIDGKFINHDQYRFVAFNAGPRICLGKDLAYLQMKTIAAAVLLRHRLTVVPGHKVEQKMS 508

Query: 574 LTFYMKNGLKVNLIKRE 578
           LT +MKNGL VNL KR+
Sbjct: 509 LTLFMKNGLLVNLYKRD 514

BLAST of Cla97C09G166850 vs. ExPASy Swiss-Prot
Match: O23066 (Cytochrome P450 86A2 OS=Arabidopsis thaliana OX=3702 GN=CYP86A2 PE=1 SV=1)

HSP 1 Score: 458.4 bits (1178), Expect = 1.2e-127
Identity = 238/492 (48.37%), Postives = 323/492 (65.65%), Query Frame = 0

Query: 94  GLPIWPIVGMLPALITAARGNLYDWITDLLRHHKGTFQ---FRGPWLTSLN--CVVTADP 153
           G  +WP++G LP LI   R  ++DWIT+ LR   GT+Q      P+L        VT DP
Sbjct: 29  GPRVWPVLGSLPGLI-EQRDRMHDWITENLRACGGTYQTCICAVPFLAKKQGLVTVTCDP 88

Query: 154 RNLEHLLKTKFSSYPKGPFFRDTVRDLLGDGIFGADDETWQRQRKVASIEFHSARFRQMT 213
           +N+EH+LKT+F +YPKGP ++    D LG GIF +D +TW  QRK A++EF +   RQ  
Sbjct: 89  KNIEHMLKTRFDNYPKGPTWQAVFHDFLGQGIFNSDGDTWLFQRKTAALEFTTRTLRQAM 148

Query: 214 VESLVELVHLRLEAVLEDFAGRSATVDLQDVLLRLTFDNVCMIAFGVDPGCLRGGLPEIP 273
              +   + LR   +LE        VDLQD++LRLTFDN+C +AFG D      GLPE  
Sbjct: 149 GRWVNRGIKLRFCPILETAQNNYEPVDLQDLILRLTFDNICGLAFGKDTRTCAPGLPENG 208

Query: 274 FAKAFEDATEVTVIRFITPTFVWKAMRYLNLGMEKKLKQSIKAVDEFADNVIRTRKRELS 333
           FA AF+ ATE ++ RFI P F+W+  ++L LG+E  L +S+  +D + D VI TRK+EL 
Sbjct: 209 FASAFDRATEASLQRFILPEFLWRLKKWLGLGLEVSLSRSLGEIDGYLDAVINTRKQEL- 268

Query: 334 TAAQPQADNQR-SDLLTVFMKLRDEQGRPFSDKFLRDICVNFILAGRDTSSVALSWFFWL 393
             +Q ++  QR  DLL+ FMK +D+    +S+ FLR + +NFILAGRDTSSVALSWFFWL
Sbjct: 269 -LSQRESGVQRHDDLLSRFMKKKDQS---YSETFLRHVALNFILAGRDTSSVALSWFFWL 328

Query: 394 LDQNPHVEDKILAEIGRIVGEREEAKTRELRFDLLVFKPEEIKKMEYLHAAISEALRLYP 453
           +  +P VEDKI+ EI  ++ E           + L F  +E+ ++ YL AA+SE LRLYP
Sbjct: 329 ITTHPTVEDKIVREICSVLIETRGTDVSSWTAEPLEF--DEVDRLVYLKAALSETLRLYP 388

Query: 454 SVPVDHKEVVEDDVFPDGTILKKGTKIIYAIYAMGRMEAIWGKDCREFKPERWMR--DGR 513
           SVP D K VV DD+ PDGT +  G+ + Y+IYA GRM++ WG+DC EFKPERW+   DG+
Sbjct: 389 SVPEDSKHVVNDDILPDGTFVPAGSSVTYSIYAAGRMKSTWGEDCLEFKPERWISPDDGK 448

Query: 514 FMSESAYRFTAFNGGPRLCLGKDFAYYQMKYVAASIMSRYRVAVVTGHSVEPKLALTFYM 573
           F++   YRF AFN GPR+CLGKD AY QMK +AA+++ R+R+ V  GH VE K++LT +M
Sbjct: 449 FVNHDQYRFVAFNAGPRICLGKDLAYLQMKTIAAAVLLRHRLTVAPGHKVEQKMSLTLFM 508

Query: 574 KNGLKVNLIKRE 578
           KNGL VN+ KR+
Sbjct: 509 KNGLLVNVHKRD 512

BLAST of Cla97C09G166850 vs. ExPASy TrEMBL
Match: A0A1S3CEY8 (LOW QUALITY PROTEIN: cytochrome P450 86B1-like OS=Cucumis melo OX=3656 GN=LOC103499965 PE=3 SV=1)

HSP 1 Score: 1073.2 bits (2774), Expect = 4.4e-310
Identity = 537/589 (91.17%), Postives = 558/589 (94.74%), Query Frame = 0

Query: 1   MVSAVLYCMTIPLILTFFQQKTSSMFSHAFPFLHHKPMMDSGNLSSSSPTAALPPPISVG 60
           M+ A LYCMTI LIL FF  KTSSMFSHAFPFLHHKP+MDSGNLSSSS +++LPP ISV 
Sbjct: 1   MLFAALYCMTIQLILHFFTTKTSSMFSHAFPFLHHKPIMDSGNLSSSS-SSSLPPSISVR 60

Query: 61  N-GLRLLLDVQILEISFALFVFLVIHVIRQKRRYGLPIWPIVGMLPALITAARGNLYDWI 120
           + GL+ LLDVQILEISFALFVFLVIHV+RQKRR+GLPIWPIVGMLPALITA RGNLY+WI
Sbjct: 61  HFGLQSLLDVQILEISFALFVFLVIHVVRQKRRHGLPIWPIVGMLPALITATRGNLYEWI 120

Query: 121 TDLLRHHKGTFQFRGPWLTSLNCVVTADPRNLEHLLKTKFSSYPKGPFFRDTVRDLLGDG 180
           TDLL  HKGTFQFRGPWLTSLNCVVTADPRNLEHLLKTKFSSYPKG FFRDTVRDLLGDG
Sbjct: 121 TDLLCRHKGTFQFRGPWLTSLNCVVTADPRNLEHLLKTKFSSYPKGSFFRDTVRDLLGDG 180

Query: 181 IFGADDETWQRQRKVASIEFHSARFRQMTVESLVELVHLRLEAVLEDFAGRSATVDLQDV 240
           IFGADDETWQRQRKVASIEFHSARFRQMTVESLVEL H RLEAVLEDFAG+S TVDLQDV
Sbjct: 181 IFGADDETWQRQRKVASIEFHSARFRQMTVESLVELAHSRLEAVLEDFAGKSTTVDLQDV 240

Query: 241 LLRLTFDNVCMIAFGVDPGCLRGGLPEIPFAKAFEDATEVTVIRFITPTFVWKAMRYLNL 300
           LLRLTFDNVCMIAFGVDPGCLRGGLPEIPFAKAFEDATEVTV+RFITPTF+WKAMRYLN+
Sbjct: 241 LLRLTFDNVCMIAFGVDPGCLRGGLPEIPFAKAFEDATEVTVMRFITPTFIWKAMRYLNI 300

Query: 301 GMEKKLKQSIKAVDEFADNVIRTRKRELSTAAQPQADNQRSDLLTVFMKLRDEQGRPFSD 360
           G+EKKLKQSIKAVDEFA+NVI+TRKRELST A+ QADN+RSDLLTVFMKLRDEQGRPFSD
Sbjct: 301 GIEKKLKQSIKAVDEFAENVIQTRKRELSTPAESQADNKRSDLLTVFMKLRDEQGRPFSD 360

Query: 361 KFLRDICVNFILAGRDTSSVALSWFFWLLDQNPHVEDKILAEIGRIVGEREEAKTRELRF 420
           KFLRDICVNFILAGRDTSSVALSWFFWLLDQNP VEDKILAEI RIVGEREEAKTRELRF
Sbjct: 361 KFLRDICVNFILAGRDTSSVALSWFFWLLDQNPDVEDKILAEICRIVGEREEAKTRELRF 420

Query: 421 DLLVFKPEEIKKMEYLHAAISEALRLYPSVPVDHKEVVEDDVFPDGTILKKGTKIIYAIY 480
           D LVFKPEE KKMEYLHAAISEALRLYPSVP DHKEVVEDD FPDGTILKKG KIIYAIY
Sbjct: 421 DSLVFKPEEXKKMEYLHAAISEALRLYPSVPFDHKEVVEDDTFPDGTILKKGMKIIYAIY 480

Query: 481 AMGRMEAIWGKDCREFKPERWMRDGRFMSESAYRFTAFNGGPRLCLGKDFAYYQMKYVAA 540
           AMGRMEAIWGKDCREFKPERWMRDGRFMSES YRFTAFNGGPRLCLGKDFAYYQMKYVAA
Sbjct: 481 AMGRMEAIWGKDCREFKPERWMRDGRFMSESVYRFTAFNGGPRLCLGKDFAYYQMKYVAA 540

Query: 541 SIMSRYRVAVVTGHSVEPKLALTFYMKNGLKVNLIKREEGQIRKYMKLN 589
           SI+SRYRVAVV GH VEPKL+LTFYMK GLKVNLIKREEGQIRKYMKLN
Sbjct: 541 SIISRYRVAVVAGHPVEPKLSLTFYMKKGLKVNLIKREEGQIRKYMKLN 588

BLAST of Cla97C09G166850 vs. ExPASy TrEMBL
Match: A0A6J1GQ36 (cytochrome P450 86B1-like OS=Cucurbita moschata OX=3662 GN=LOC111456469 PE=3 SV=1)

HSP 1 Score: 1036.6 bits (2679), Expect = 4.1e-299
Identity = 514/565 (90.97%), Postives = 536/565 (94.87%), Query Frame = 0

Query: 25  MFSHAFPFLHHKPMMDSGNLSSSSPTAALPPPISVGN-GLRLLLDVQILEISFALFVFLV 84
           MFSHA PFLHHKPMMDSGNLSSSS   ALP  +S GN GL  LLD QILEISFALFVFL 
Sbjct: 1   MFSHAIPFLHHKPMMDSGNLSSSS---ALPALVSAGNFGLWPLLDFQILEISFALFVFLA 60

Query: 85  IHVIRQKRRYGLPIWPIVGMLPALITAARGNLYDWITDLLRHHKGTFQFRGPWLTSLNCV 144
           IHV+RQ+RRYGLP+WPIVGMLP+LITAARGNLY+WITDLLRHHKGTFQFRGPW +SLNCV
Sbjct: 61  IHVVRQQRRYGLPVWPIVGMLPSLITAARGNLYEWITDLLRHHKGTFQFRGPWFSSLNCV 120

Query: 145 VTADPRNLEHLLKTKFSSYPKGPFFRDTVRDLLGDGIFGADDETWQRQRKVASIEFHSAR 204
           VTADPRNLEHLLKTKF SYPKGPFFRDTVRDLLGDGIFGADDETWQRQRKVASIEFHSAR
Sbjct: 121 VTADPRNLEHLLKTKFPSYPKGPFFRDTVRDLLGDGIFGADDETWQRQRKVASIEFHSAR 180

Query: 205 FRQMTVESLVELVHLRLEAVLEDFAGRSATVDLQDVLLRLTFDNVCMIAFGVDPGCLRGG 264
           FRQMTVESLVELVH RLE VLEDF GR   VDLQDVLLRLTFDNVCMIAFGVDPGCLRGG
Sbjct: 181 FRQMTVESLVELVHSRLETVLEDFLGRRTAVDLQDVLLRLTFDNVCMIAFGVDPGCLRGG 240

Query: 265 LPEIPFAKAFEDATEVTVIRFITPTFVWKAMRYLNLGMEKKLKQSIKAVDEFADNVIRTR 324
           LPEIPFAKAFEDATEVTV+RFITPTFVWK +RYLNLG+EKKLKQSI+AVDEFA++VI+TR
Sbjct: 241 LPEIPFAKAFEDATEVTVLRFITPTFVWKILRYLNLGVEKKLKQSIQAVDEFAEDVIQTR 300

Query: 325 KRELSTAAQPQADNQRSDLLTVFMKLRDEQGRPFSDKFLRDICVNFILAGRDTSSVALSW 384
           KRELST AQPQA+NQRSDLLTVFM+LRDEQ RPFSDKFLRDICVNFILAGRDTSSVALSW
Sbjct: 301 KRELSTTAQPQAENQRSDLLTVFMRLRDEQRRPFSDKFLRDICVNFILAGRDTSSVALSW 360

Query: 385 FFWLLDQNPHVEDKILAEIGRIVGEREEAKTRELRFDLLVFKPEEIKKMEYLHAAISEAL 444
           FFWLLD+NP VEDKILAEI RIVGEREEAKTRE RFD LVFKPEEIKKMEYLHAAISEAL
Sbjct: 361 FFWLLDRNPDVEDKILAEIYRIVGEREEAKTREFRFDSLVFKPEEIKKMEYLHAAISEAL 420

Query: 445 RLYPSVPVDHKEVVEDDVFPDGTILKKGTKIIYAIYAMGRMEAIWGKDCREFKPERWMRD 504
           RLYPSVPVDHKEVVEDDVFPDGTILKKGTKIIYAIYAMGRMEAIWGKDCREFKPERWM+D
Sbjct: 421 RLYPSVPVDHKEVVEDDVFPDGTILKKGTKIIYAIYAMGRMEAIWGKDCREFKPERWMKD 480

Query: 505 GRFMSESAYRFTAFNGGPRLCLGKDFAYYQMKYVAASIMSRYRVAVVTGHSVEPKLALTF 564
           GRFMSESAYRFTAFNGGPRLCLG+DFAYYQMKYVAASI+SRYRVAVV GH VEPKLALTF
Sbjct: 481 GRFMSESAYRFTAFNGGPRLCLGRDFAYYQMKYVAASIISRYRVAVVKGHPVEPKLALTF 540

Query: 565 YMKNGLKVNLIKREEGQIRKYMKLN 589
           YMK+GLKVNLIKREEGQIRKY+KLN
Sbjct: 541 YMKDGLKVNLIKREEGQIRKYVKLN 562

BLAST of Cla97C09G166850 vs. ExPASy TrEMBL
Match: A0A6J1DJX8 (cytochrome P450 86B1 OS=Momordica charantia OX=3673 GN=LOC111021175 PE=3 SV=1)

HSP 1 Score: 1025.0 bits (2649), Expect = 1.2e-295
Identity = 507/565 (89.73%), Postives = 531/565 (93.98%), Query Frame = 0

Query: 25  MFSHAFPFLHHKPMMDSGNLSSSSPTAALPPPISVGN-GLRLLLDVQILEISFALFVFLV 84
           MFS+A PFLHHKPMMDS NLSSSS  ++LPP IS G  G R LLDVQILEI FALFVFL 
Sbjct: 1   MFSNAIPFLHHKPMMDSSNLSSSS--SSLPPLISSGKFGFRQLLDVQILEIFFALFVFLA 60

Query: 85  IHVIRQKRRYGLPIWPIVGMLPALITAARGNLYDWITDLLRHHKGTFQFRGPWLTSLNCV 144
           IHV+RQKRRYGLP+WP+VGMLPALITAARGNLY+WIT+LLR HKGTFQF GPWL+SLNCV
Sbjct: 61  IHVVRQKRRYGLPVWPVVGMLPALITAARGNLYEWITELLRRHKGTFQFHGPWLSSLNCV 120

Query: 145 VTADPRNLEHLLKTKFSSYPKGPFFRDTVRDLLGDGIFGADDETWQRQRKVASIEFHSAR 204
           VTADPRNLEHLLKTKF SYPKGPFFRDTVRDLLGDGIFGADDETWQRQRK ASIEFHSA+
Sbjct: 121 VTADPRNLEHLLKTKFPSYPKGPFFRDTVRDLLGDGIFGADDETWQRQRKTASIEFHSAK 180

Query: 205 FRQMTVESLVELVHLRLEAVLEDFAGRSATVDLQDVLLRLTFDNVCMIAFGVDPGCLRGG 264
           FR+MTVESLVELVH RLE VLED+AGR A VDLQDVLLRLTFDNVCMIAFGVDPGCLRGG
Sbjct: 181 FRRMTVESLVELVHRRLEGVLEDYAGRPAAVDLQDVLLRLTFDNVCMIAFGVDPGCLRGG 240

Query: 265 LPEIPFAKAFEDATEVTVIRFITPTFVWKAMRYLNLGMEKKLKQSIKAVDEFADNVIRTR 324
           LPEIPFAKAFEDATE TV+RFITPTF+WKAMRYLNLGMEKKLK SIKAVDEFA+NVIRTR
Sbjct: 241 LPEIPFAKAFEDATEATVLRFITPTFIWKAMRYLNLGMEKKLKLSIKAVDEFAENVIRTR 300

Query: 325 KRELSTAAQPQADNQRSDLLTVFMKLRDEQGRPFSDKFLRDICVNFILAGRDTSSVALSW 384
           KRELS AAQPQA+NQRSDLLTVFM+LRDEQGRPFSDKFLRDICVNFILAGRDTSSVAL+W
Sbjct: 301 KRELSAAAQPQAENQRSDLLTVFMRLRDEQGRPFSDKFLRDICVNFILAGRDTSSVALTW 360

Query: 385 FFWLLDQNPHVEDKILAEIGRIVGEREEAKTRELRFDLLVFKPEEIKKMEYLHAAISEAL 444
           FFWLLDQNP VE+K LAEI RIVGEREEA+T ELRFD L+FKPEEIKKMEYLHAAISEAL
Sbjct: 361 FFWLLDQNPDVEEKTLAEICRIVGEREEAQTGELRFDSLMFKPEEIKKMEYLHAAISEAL 420

Query: 445 RLYPSVPVDHKEVVEDDVFPDGTILKKGTKIIYAIYAMGRMEAIWGKDCREFKPERWMRD 504
           RLYPSVPVDHKEVVEDDVFPDGTILKKGTKIIYAIYAMGRMEAIWGKDCREFKPERWMRD
Sbjct: 421 RLYPSVPVDHKEVVEDDVFPDGTILKKGTKIIYAIYAMGRMEAIWGKDCREFKPERWMRD 480

Query: 505 GRFMSESAYRFTAFNGGPRLCLGKDFAYYQMKYVAASIMSRYRVAVVTGHSVEPKLALTF 564
           GRFMSESAYRF AFNGGPRLCLGKDFAYYQMKYVAASI+ RYRV V  GH VEPKLALTF
Sbjct: 481 GRFMSESAYRFAAFNGGPRLCLGKDFAYYQMKYVAASIIRRYRVTVAAGHPVEPKLALTF 540

Query: 565 YMKNGLKVNLIKREEGQIRKYMKLN 589
           YMK+GLKVNLIKREEGQIRK+MKLN
Sbjct: 541 YMKHGLKVNLIKREEGQIRKFMKLN 563

BLAST of Cla97C09G166850 vs. ExPASy TrEMBL
Match: A0A5A7UTT4 (Cytochrome P450 86B1-like OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold459G00200 PE=3 SV=1)

HSP 1 Score: 1019.6 bits (2635), Expect = 5.2e-294
Identity = 509/551 (92.38%), Postives = 528/551 (95.83%), Query Frame = 0

Query: 39  MDSGNLSSSSPTAALPPPISVGN-GLRLLLDVQILEISFALFVFLVIHVIRQKRRYGLPI 98
           MDSGNLSSSS +++LPP ISV + GL+ LLDVQILEISFALFVFLVIHV+RQKRR+GLPI
Sbjct: 1   MDSGNLSSSS-SSSLPPSISVRHFGLQSLLDVQILEISFALFVFLVIHVVRQKRRHGLPI 60

Query: 99  WPIVGMLPALITAARGNLYDWITDLLRHHKGTFQFRGPWLTSLNCVVTADPRNLEHLLKT 158
           WPIVGMLPALITA RGNLY+WITDLL  HKGTFQFRGPWLTSLNCVVTADPRNLEHLLKT
Sbjct: 61  WPIVGMLPALITATRGNLYEWITDLLCRHKGTFQFRGPWLTSLNCVVTADPRNLEHLLKT 120

Query: 159 KFSSYPKGPFFRDTVRDLLGDGIFGADDETWQRQRKVASIEFHSARFRQMTVESLVELVH 218
           KFSSYPKG FFRDTVRDLLGDGIFGADDETWQRQRKVASIEFHSARFRQMTVESLVELVH
Sbjct: 121 KFSSYPKGSFFRDTVRDLLGDGIFGADDETWQRQRKVASIEFHSARFRQMTVESLVELVH 180

Query: 219 LRLEAVLEDFAGRSATVDLQDVLLRLTFDNVCMIAFGVDPGCLRGGLPEIPFAKAFEDAT 278
            RLEAVLEDFAG+S TVDLQDVLLRLTFDNVCMIAFGVDPGCLRGGLPEIPFAKAFEDAT
Sbjct: 181 SRLEAVLEDFAGKSTTVDLQDVLLRLTFDNVCMIAFGVDPGCLRGGLPEIPFAKAFEDAT 240

Query: 279 EVTVIRFITPTFVWKAMRYLNLGMEKKLKQSIKAVDEFADNVIRTRKRELSTAAQPQADN 338
           EVTV+RFITPTF+WKAMRYLN+G+EKKLKQSIKAVDEFA+NVI+TRKRELST A+ QADN
Sbjct: 241 EVTVMRFITPTFIWKAMRYLNIGIEKKLKQSIKAVDEFAENVIQTRKRELSTPAESQADN 300

Query: 339 QRSDLLTVFMKLRDEQGRPFSDKFLRDICVNFILAGRDTSSVALSWFFWLLDQNPHVEDK 398
           +RSDLLTVFMKLRDEQGRPFSDKFLRDICVNFILAGRDTSSVALSWFFWLLDQNP VEDK
Sbjct: 301 KRSDLLTVFMKLRDEQGRPFSDKFLRDICVNFILAGRDTSSVALSWFFWLLDQNPDVEDK 360

Query: 399 ILAEIGRIVGEREEAKTRELRFDLLVFKPEEIKKMEYLHAAISEALRLYPSVPVDHKEVV 458
           ILAEI RIVGEREEAKTRELRFD LVFKPEEIKKMEYLHAAISEALRLYPSVP DHKEVV
Sbjct: 361 ILAEICRIVGEREEAKTRELRFDSLVFKPEEIKKMEYLHAAISEALRLYPSVPFDHKEVV 420

Query: 459 EDDVFPDGTILKKGTKIIYAIYAMGRMEAIWGKDCREFKPERWMRDGRFMSESAYRFTAF 518
           EDD FPDGTILKKG KIIYAIYAMGRMEAIWGKDCREFKPERWMRDGRFMSES YRFTAF
Sbjct: 421 EDDTFPDGTILKKGMKIIYAIYAMGRMEAIWGKDCREFKPERWMRDGRFMSESVYRFTAF 480

Query: 519 NGGPRLCLGKDFAYYQMKYVAASIMSRYRVAVVTGHSVEPKLALTFYMKNGLKVNLIKRE 578
           NGGPRLCLGKDFAYYQMKYVAASI+SRYRVAVV GH VEPKL+LTFYMK GLKVNLIKRE
Sbjct: 481 NGGPRLCLGKDFAYYQMKYVAASIISRYRVAVVAGHPVEPKLSLTFYMKKGLKVNLIKRE 540

Query: 579 EGQIRKYMKLN 589
           EGQIRKYMKLN
Sbjct: 541 EGQIRKYMKLN 550

BLAST of Cla97C09G166850 vs. ExPASy TrEMBL
Match: A0A6J1JMG1 (cytochrome P450 86B1-like OS=Cucurbita maxima OX=3661 GN=LOC111488206 PE=3 SV=1)

HSP 1 Score: 1017.7 bits (2630), Expect = 2.0e-293
Identity = 508/565 (89.91%), Postives = 529/565 (93.63%), Query Frame = 0

Query: 25  MFSHAFPFLHHKPMMDSGNLSSSSPTAALPPPISVGN-GLRLLLDVQILEISFALFVFLV 84
           MFSHA PFLHHKP+MDSGNLSSSS   ALP  +S  N  L  LLDVQILE+SFALFVFL 
Sbjct: 1   MFSHAIPFLHHKPIMDSGNLSSSS---ALPAVVSAENFRLWPLLDVQILELSFALFVFLA 60

Query: 85  IHVIRQKRRYGLPIWPIVGMLPALITAARGNLYDWITDLLRHHKGTFQFRGPWLTSLNCV 144
           IHVIRQ+RRYGL +WPIVGMLP+LITAARGNLY+WITDLLR HKGTFQFRGPW +SLNCV
Sbjct: 61  IHVIRQQRRYGLSVWPIVGMLPSLITAARGNLYEWITDLLRRHKGTFQFRGPWFSSLNCV 120

Query: 145 VTADPRNLEHLLKTKFSSYPKGPFFRDTVRDLLGDGIFGADDETWQRQRKVASIEFHSAR 204
           VTADPRNLEHLLKTKF  YPKG FFRDTVRDLLGDGIFGADDETWQRQRKVASIEFHSAR
Sbjct: 121 VTADPRNLEHLLKTKFPYYPKGSFFRDTVRDLLGDGIFGADDETWQRQRKVASIEFHSAR 180

Query: 205 FRQMTVESLVELVHLRLEAVLEDFAGRSATVDLQDVLLRLTFDNVCMIAFGVDPGCLRGG 264
           FRQMTVESLVELVH RLE VLEDF  R   VDLQDVLLRLTFDNVCMIAFGVDPGCLRGG
Sbjct: 181 FRQMTVESLVELVHSRLETVLEDFVERRTAVDLQDVLLRLTFDNVCMIAFGVDPGCLRGG 240

Query: 265 LPEIPFAKAFEDATEVTVIRFITPTFVWKAMRYLNLGMEKKLKQSIKAVDEFADNVIRTR 324
           LPEIPFAKAFEDATEVTV+RFITPTFVWK +RYLNLG+EKKLKQSIK VDEFA++VI+TR
Sbjct: 241 LPEIPFAKAFEDATEVTVLRFITPTFVWKILRYLNLGVEKKLKQSIKTVDEFAEDVIQTR 300

Query: 325 KRELSTAAQPQADNQRSDLLTVFMKLRDEQGRPFSDKFLRDICVNFILAGRDTSSVALSW 384
           KRELST AQPQA+NQRSDLLTVFM+LRDEQGRPFSDKFLRDICVNFILAGRDTSSVALSW
Sbjct: 301 KRELSTTAQPQANNQRSDLLTVFMRLRDEQGRPFSDKFLRDICVNFILAGRDTSSVALSW 360

Query: 385 FFWLLDQNPHVEDKILAEIGRIVGEREEAKTRELRFDLLVFKPEEIKKMEYLHAAISEAL 444
           FFWLLDQNP VE+KILAEI RIVGER EAKTRE RFD LVFKPEEIKKMEYLHAAISEAL
Sbjct: 361 FFWLLDQNPDVENKILAEIYRIVGERGEAKTREFRFDSLVFKPEEIKKMEYLHAAISEAL 420

Query: 445 RLYPSVPVDHKEVVEDDVFPDGTILKKGTKIIYAIYAMGRMEAIWGKDCREFKPERWMRD 504
           RLYPSVPVDHKEVVEDDVFPDGTILKKGTKIIYAIYAMGRMEAIWGKDCREFKPERWM+D
Sbjct: 421 RLYPSVPVDHKEVVEDDVFPDGTILKKGTKIIYAIYAMGRMEAIWGKDCREFKPERWMKD 480

Query: 505 GRFMSESAYRFTAFNGGPRLCLGKDFAYYQMKYVAASIMSRYRVAVVTGHSVEPKLALTF 564
           GRFMSESAYRFTAFNGGPRLCLG+DFAYYQMKYVAASI+SRYRVAVV GH VEPKLALTF
Sbjct: 481 GRFMSESAYRFTAFNGGPRLCLGRDFAYYQMKYVAASIISRYRVAVVKGHPVEPKLALTF 540

Query: 565 YMKNGLKVNLIKREEGQIRKYMKLN 589
           YMK+GLKVNLIKREEGQIRKYMKLN
Sbjct: 541 YMKDGLKVNLIKREEGQIRKYMKLN 562

BLAST of Cla97C09G166850 vs. TAIR 10
Match: AT5G23190.1 (cytochrome P450, family 86, subfamily B, polypeptide 1 )

HSP 1 Score: 761.1 bits (1964), Expect = 6.4e-220
Identity = 376/540 (69.63%), Postives = 435/540 (80.56%), Query Frame = 0

Query: 45  SSSSPTAALPPPISVGNGLRLLLDVQILEISFALFVFLVIHVIRQKRRYGLPIWPIVGML 104
           SSSS +  L     V   L LL DVQILE+  A+FVF+ IH +RQK+  GLP+WP +GML
Sbjct: 17  SSSSSSDPL-----VSRRLFLLRDVQILELLIAIFVFVAIHALRQKKYQGLPVWPFLGML 76

Query: 105 PALITAARGNLYDWITDLLRHHKGTFQFRGPWLTSLNCVVTADPRNLEHLLKTKFSSYPK 164
           P+L    RGN+Y+W++D+L    GTFQFRGPW +SLN  +T DPRN+EHLLK +FS +PK
Sbjct: 77  PSLAFGLRGNIYEWLSDVLCLQNGTFQFRGPWFSSLNSTITCDPRNVEHLLKNRFSVFPK 136

Query: 165 GPFFRDTVRDLLGDGIFGADDETWQRQRKVASIEFHSARFRQMTVESLVELVHLRLEAVL 224
           G +FRD +RDLLGDGIF ADDETWQRQRK ASIEFHSA+FRQ+T +SL ELVH RL  VL
Sbjct: 137 GSYFRDNLRDLLGDGIFNADDETWQRQRKTASIEFHSAKFRQLTTQSLFELVHKRLLPVL 196

Query: 225 EDFAGRSATVDLQDVLLRLTFDNVCMIAFGVDPGCLRGGLPEIPFAKAFEDATEVTVIRF 284
           E     S+ +DLQDVLLRLTFDNVCMIAFGVDPGCL    P IPFAKAFEDATE  V+RF
Sbjct: 197 ETSVKSSSPIDLQDVLLRLTFDNVCMIAFGVDPGCLGPDQPVIPFAKAFEDATEAAVVRF 256

Query: 285 ITPTFVWKAMRYLNLGMEKKLKQSIKAVDEFADNVIRTRKRELSTAAQPQADNQRSDLLT 344
           + PT VWK MRYL++G EKKLK+SIK VD+FAD VIRTRK+ELS   +     +RSDLLT
Sbjct: 257 VMPTCVWKFMRYLDIGTEKKLKESIKGVDDFADEVIRTRKKELSLEGE---TTKRSDLLT 316

Query: 345 VFMKLRDEQGRPFSDKFLRDICVNFILAGRDTSSVALSWFFWLLDQNPHVEDKILAEIGR 404
           VFM LRDE+G  FSDKFLRDICVNFILAGRDTSSVALSWFFWLL++NP VE+KI+ E+ +
Sbjct: 317 VFMGLRDEKGESFSDKFLRDICVNFILAGRDTSSVALSWFFWLLEKNPEVEEKIMVEMCK 376

Query: 405 IVGEREEAKTRELRFDLLVFKPEEIKKMEYLHAAISEALRLYPSVPVDHKEVVEDDVFPD 464
           I+ +R++    E      VF PEEIKKM+YL AA+SEALRLYPSVPVDHKEV EDDVFPD
Sbjct: 377 ILRQRDDHGNAEKSDYEPVFGPEEIKKMDYLQAALSEALRLYPSVPVDHKEVQEDDVFPD 436

Query: 465 GTILKKGTKIIYAIYAMGRMEAIWGKDCREFKPERWMRDGRFMSESAYRFTAFNGGPRLC 524
           GT+LKKG K+IYAIYAMGRMEAIWGKDC EF+PERW+RDGRFMSESAY+FTAFNGGPRLC
Sbjct: 437 GTMLKKGDKVIYAIYAMGRMEAIWGKDCLEFRPERWLRDGRFMSESAYKFTAFNGGPRLC 496

Query: 525 LGKDFAYYQMKYVAASIMSRYRVAVVTGHSVEPKLALTFYMKNGLKVNLIKREEGQIRKY 584
           LGKDFAYYQMK  AA+I+ RY+V VV GH VEPKLALT YMK+GL VNLI R   +I +Y
Sbjct: 497 LGKDFAYYQMKSTAAAIVYRYKVKVVNGHKVEPKLALTMYMKHGLMVNLINRSVSEIDQY 548

BLAST of Cla97C09G166850 vs. TAIR 10
Match: AT5G08250.1 (Cytochrome P450 superfamily protein )

HSP 1 Score: 716.8 bits (1849), Expect = 1.4e-206
Identity = 346/493 (70.18%), Postives = 410/493 (83.16%), Query Frame = 0

Query: 95  LPIWPIVGMLPALITAARGNLYDWITDLLRHHKGTFQFRGPWLTSLNCVVTADPRNLEHL 154
           +P+WP+VGMLP+LI+A R N+Y+W++D+L    GTF+FRGPW ++LNCVVT DPRN+EHL
Sbjct: 1   MPVWPLVGMLPSLISAVRSNIYEWLSDVLISQNGTFRFRGPWFSTLNCVVTCDPRNVEHL 60

Query: 155 LKTKFSSYPKGPFFRDTVRDLLGDGIFGADDETWQRQRKVASIEFHSARFRQMTVESLVE 214
           LKT+FS YPKG +FR+T++DLLGDGIF  DD TWQRQRK AS+EFHSA+FRQ+T +SL E
Sbjct: 61  LKTRFSIYPKGSYFRETMQDLLGDGIFNTDDGTWQRQRKAASVEFHSAKFRQLTSQSLHE 120

Query: 215 LVHLRLEAVLEDFAGRSATVDLQDVLLRLTFDNVCMIAFGVDPGCLRGGLPEIPFAKAFE 274
           LVH RL  VLE     S  +DLQD+LLRLTFDNVCMIAFGVDPGCL   LPEIPFAKAFE
Sbjct: 121 LVHNRLLPVLE----TSGKIDLQDILLRLTFDNVCMIAFGVDPGCLSPKLPEIPFAKAFE 180

Query: 275 DATEVTVIRFITPTFVWKAMRYLNLGMEKKLKQSIKAVDEFADNVIRTRKRELSTAAQPQ 334
           DATE TV+RF+ P FVWK MR LNLG EKKLK+SI  VD+FA+ VIRTRK+E+S   +  
Sbjct: 181 DATEATVVRFVMPKFVWKLMRSLNLGTEKKLKESINGVDDFAEEVIRTRKKEMSLETEIA 240

Query: 335 ADNQRSDLLTVFMKLRDEQGRPFSDKFLRDICVNFILAGRDTSSVALSWFFWLLDQNPHV 394
              +R DLLT+FM LRDE G+ FSDKFLRDICVNFILAGRDTSSVALSWFFWL+++NP V
Sbjct: 241 ---KRPDLLTIFMGLRDENGQKFSDKFLRDICVNFILAGRDTSSVALSWFFWLIEKNPEV 300

Query: 395 EDKILAEIGRIVGER-EEAKTRELRFDLLVFKPEEIKKMEYLHAAISEALRLYPSVPVDH 454
           E+KI+  I +I+ +R +   T++      VF+PEEIKKM+YL AA+SE LRLYPSVPVDH
Sbjct: 301 EEKIMMGICKILEQRVDHGDTKKNMEYEPVFRPEEIKKMDYLQAALSETLRLYPSVPVDH 360

Query: 455 KEVVEDDVFPDGTILKKGTKIIYAIYAMGRMEAIWGKDCREFKPERWMRDGRFMSESAYR 514
           KEV+EDDVFPDGT LKKG K+IYAIYAMGRME IWGKDCREFKPERW+RDGR+MSESAY+
Sbjct: 361 KEVLEDDVFPDGTKLKKGEKVIYAIYAMGRMETIWGKDCREFKPERWLRDGRYMSESAYK 420

Query: 515 FTAFNGGPRLCLGKDFAYYQMKYVAASIMSRYRVAV--VTGHSVEPKLALTFYMKNGLKV 574
           FTAFNGGPRLCLGKDFAYYQM+YVAA+I+ RY+V V    GH VEPK+ALT YMK+GLKV
Sbjct: 421 FTAFNGGPRLCLGKDFAYYQMRYVAAAIIYRYKVRVDDKGGHKVEPKMALTMYMKHGLKV 480

Query: 575 NLIKREEGQIRKY 585
           N++KR   +I  Y
Sbjct: 481 NMVKRSVSEIDHY 486

BLAST of Cla97C09G166850 vs. TAIR 10
Match: AT1G24540.1 (cytochrome P450, family 86, subfamily C, polypeptide 1 )

HSP 1 Score: 500.4 bits (1287), Expect = 2.0e-141
Identity = 243/517 (47.00%), Postives = 349/517 (67.50%), Query Frame = 0

Query: 69  VQILEISFALFVFLVIHVIRQK--RRYGLPIWPIVGMLPALITAARGNLYDWITDLLRHH 128
           +++ +++ AL    ++  +R+K   + G  +WP++G++P ++   + +L+ W T  +   
Sbjct: 15  LRLSDVALALVGLFLLSYLREKLVSKGGPVMWPVLGIIP-MLALNKHDLFTWCTRCVVRS 74

Query: 129 KGTFQFRGPWLTSLNCVVTADPRNLEHLLKTKFSSYPKGPFFRDTVRDLLGDGIFGADDE 188
            GTF +RG W      ++TADP N+EH+LKT F +YPKG F+R+  RDLL DGIF ADDE
Sbjct: 75  GGTFHYRGIWFGGAYGIMTADPANVEHILKTNFKNYPKGAFYRERFRDLLEDGIFNADDE 134

Query: 189 TWQRQRKVASIEFHSARFRQMTVESLVELVHLRLEAVLEDFAGRSATVDLQDVLLRLTFD 248
            W+ +R+VA  E HS+RF + T  ++ +LV  +L  ++E+ +      DLQD+LLR TFD
Sbjct: 135 LWKEERRVAKTEMHSSRFLEHTFTTMRDLVDQKLVPLMENLSTSKRVFDLQDLLLRFTFD 194

Query: 249 NVCMIAFGVDPGCLRGGLPEIPFAKAFEDATEVTVIRFITPTFVWKAMRYLNLGMEKKLK 308
           N+C+ AFGV PG L  GLPEIPFAKAFEDATE T+ RF+ P FVWK MR+L +G E+KL 
Sbjct: 195 NICISAFGVYPGSLETGLPEIPFAKAFEDATEYTLARFLIPPFVWKPMRFLGIGYERKLN 254

Query: 309 QSIKAVDEFADNVIRTRKRELSTAAQPQADNQRSDLLTVFMKLRDE------QGRPFSDK 368
            +++ V  FA+  +R R+ ++         N  +DLL+  M+   E      +G  FSDK
Sbjct: 255 NAVRIVHAFANKTVRERRNKMRKLGNL---NDYADLLSRLMQREYEKEEDTTRGNYFSDK 314

Query: 369 FLRDICVNFILAGRDTSSVALSWFFWLLDQNPHVEDKILAEIGRIVGEREEAKTRELRFD 428
           + R+ C +FI+AGRDT+SVAL WFFWL+ ++P VE +IL EI  I  +    +T +    
Sbjct: 315 YFREFCTSFIIAGRDTTSVALVWFFWLVQKHPEVEKRILREIREIKRKLTTQETED---- 374

Query: 429 LLVFKPEEIKKMEYLHAAISEALRLYPSVPVDHKEVVEDDVFPDGTILKKGTKIIYAIYA 488
              F+ E+ ++M YL AA++E+LRLYPSVP++ K+ +EDDV PDGT +KKG +I Y++Y+
Sbjct: 375 --QFEAEDFREMVYLQAALTESLRLYPSVPMEMKQALEDDVLPDGTRVKKGARIHYSVYS 434

Query: 489 MGRMEAIWGKDCREFKPERWMRDGRFMSESAYRFTAFNGGPRLCLGKDFAYYQMKYVAAS 548
           MGR+E+IWGKD  EFKPERW+++GR +SE  +++  FNGGPRLC+GK FAY QMK VAA+
Sbjct: 435 MGRIESIWGKDWEEFKPERWIKEGRIVSEDQFKYVVFNGGPRLCVGKKFAYTQMKMVAAA 494

Query: 549 IMSRYRVAVVTGHSVEPKLALTFYMKNGLKVNLIKRE 578
           I+ RY V VV G  + PKL  T YMKNG+ V L  R+
Sbjct: 495 ILMRYSVKVVQGQEIVPKLTTTLYMKNGMNVMLQPRD 521

BLAST of Cla97C09G166850 vs. TAIR 10
Match: AT1G13140.1 (cytochrome P450, family 86, subfamily C, polypeptide 3 )

HSP 1 Score: 474.6 bits (1220), Expect = 1.2e-133
Identity = 226/507 (44.58%), Postives = 334/507 (65.88%), Query Frame = 0

Query: 73  EISFALFVFLVIHVIRQK--RRYGLPIWPIVGMLPALITAARGNLYDWITDLLRHHKGTF 132
           ++S AL    V   +R+K  ++ G  IWP+ G+ P      R ++Y W T  L+  +GTF
Sbjct: 22  DVSLALLGLFVFCCVREKVTKKLGPTIWPVFGITPEFF-FHRNDVYGWATRCLKKCRGTF 81

Query: 133 QFRGPWLTSLNCVVTADPRNLEHLLKTKFSSYPKGPFFRDTVRDLLGDGIFGADDETWQR 192
            + G WL      VT  P N+E++LKT F ++PKG FF++   DLL DGIF AD E+W+ 
Sbjct: 82  LYNGIWLGGSYGAVTCVPANVEYMLKTNFKNFPKGAFFKERFNDLLEDGIFNADAESWKE 141

Query: 193 QRKVASIEFHSARFRQMTVESLVELVHLRLEAVLEDFAGRSATVDLQDVLLRLTFDNVCM 252
           QR++   E HS RF + + ++  +LV  +L  V+E FA      DLQDVLLRLTFDN+C+
Sbjct: 142 QRRIIITEMHSTRFVEHSFQTTQDLVRKKLLKVMESFARSQEAFDLQDVLLRLTFDNICI 201

Query: 253 IAFGVDPGCLRGGLPEIPFAKAFEDATEVTVIRFITPTFVWKAMRYLNLGMEKKLKQSIK 312
              G DPG L   LP +PFA+AFE+ATE T+ RF+ P F+WK +++ ++G EK L++++ 
Sbjct: 202 AGLGDDPGTLDSDLPLVPFAQAFEEATESTMFRFMIPPFIWKPLKFFDIGYEKGLRKAVD 261

Query: 313 AVDEFADNVIRTRKRELSTAAQPQADNQRSDLLTVFMKLRD----EQGRPFSDKFLRDIC 372
            V EF D ++  R  +L    +      RSD+L+  +++      ++  P + KF R  C
Sbjct: 262 VVHEFVDKMVVDRICKLK---EEGTLGNRSDVLSRIIEIESHKTTDEKDPSTIKFFRQFC 321

Query: 373 VNFILAGRDTSSVALSWFFWLLDQNPHVEDKILAEIGRIVGEREEAKTRELRFDLLVFKP 432
            +FILAGRDTSSVAL+WFFW++ ++P VE+KI+ EI  I+ +R ++ T +   +  +F  
Sbjct: 322 TSFILAGRDTSSVALTWFFWVIQKHPEVENKIIREISEILRQRGDSPTSK---NESLFTV 381

Query: 433 EEIKKMEYLHAAISEALRLYPSVPVDHKEVVEDDVFPDGTILKKGTKIIYAIYAMGRMEA 492
           +E+  M YL AA+SE +RLYP +P++ K+ +EDDVFPDGT ++KG+++ +A YAMGRME+
Sbjct: 382 KELNDMVYLQAALSETMRLYPPIPMEMKQAIEDDVFPDGTFIRKGSRVYFATYAMGRMES 441

Query: 493 IWGKDCREFKPERWMRDGRFMSESAYRFTAFNGGPRLCLGKDFAYYQMKYVAASIMSRYR 552
           IWGKDC  FKPERW++ G F+++  +++  FN GPRLCLGK FAY QMK +AAS++SRY 
Sbjct: 442 IWGKDCESFKPERWIQSGNFVNDDQFKYVVFNAGPRLCLGKTFAYLQMKTIAASVLSRYS 501

Query: 553 VAVVTGHSVEPKLALTFYMKNGLKVNL 574
           + V   H V P++  T YM++GLKV +
Sbjct: 502 IKVAKDHVVVPRVTTTLYMRHGLKVTI 521

BLAST of Cla97C09G166850 vs. TAIR 10
Match: AT1G63710.1 (cytochrome P450, family 86, subfamily A, polypeptide 7 )

HSP 1 Score: 471.1 bits (1211), Expect = 1.3e-132
Identity = 247/517 (47.78%), Postives = 337/517 (65.18%), Query Frame = 0

Query: 67  LDVQILEISFALFVFLVIHVIRQKRRY-GLPIWPIVGMLPALITAARGNLYDWITDLLRH 126
           +D     I   L V  +I  +  +R Y G  +WP+VG LPALIT A   ++D+I D LR 
Sbjct: 1   MDGSTAAIILTLIVTYIIWFVSLRRSYKGPRVWPLVGSLPALITNAH-RMHDFIADNLRM 60

Query: 127 HKGTFQ---FRGPWLTSL--NCVVTADPRNLEHLLKTKFSSYPKGPFFRDTVRDLLGDGI 186
             GT+Q   F  P+L     +  VT DP+NLEH+LKT+F +YPKGP ++    DLLGDGI
Sbjct: 61  CGGTYQTCIFPIPFLAKKQGHVTVTCDPKNLEHILKTRFDNYPKGPSWQSVFHDLLGDGI 120

Query: 187 FGADDETWQRQRKVASIEFHSARFRQMTVESLVELVHLRLEAVLEDFAGRSATVDLQDVL 246
           F +D +TW+ QRK A++EF +   RQ     +   +  RL  +LE    R+  +DLQDVL
Sbjct: 121 FNSDGDTWRFQRKTAALEFTTRTLRQAMARWVDRAIKNRLVPILESARSRAEPIDLQDVL 180

Query: 247 LRLTFDNVCMIAFGVDPGCLRGGLPEIPFAKAFEDATEVTVIRFITPTFVWKAMRYLNLG 306
           LRLTFDN+C + FG DP  L    PE  FA AF+ ATE T+ RFI P F+WK  ++L LG
Sbjct: 181 LRLTFDNICGLTFGKDPRTLSPEFPENGFAVAFDGATEATLQRFIMPEFIWKIRKWLRLG 240

Query: 307 MEKKLKQSIKAVDEFADNVIRTRKRELSTAAQPQADNQRSDLLTVFMKLRDEQGRPFSDK 366
           +E  + +SI  VD +   +I TRK EL    Q Q +++  DLL+ FMK ++     +SDK
Sbjct: 241 LEDDMSRSISHVDNYLSEIINTRKLEL--LGQQQDESRHDDLLSRFMKKKES----YSDK 300

Query: 367 FLRDICVNFILAGRDTSSVALSWFFWLLDQNPHVEDKILAEIGRIVGEREEAKTRELRFD 426
           +L+ + +NFILAGRDTSSVA+SWFFWL+  NP VE+KI+ EI  I+ +  +    +   +
Sbjct: 301 YLKYVALNFILAGRDTSSVAMSWFFWLVSLNPRVEEKIINEICTILIKTRDTNVSKWTDE 360

Query: 427 LLVFKPEEIKKMEYLHAAISEALRLYPSVPVDHKEVVEDDVFPDGTILKKGTKIIYAIYA 486
            L F  +EI ++ YL AA+SE LRLYPSVP D K VV +DV PDGT +  G+ + Y+IY+
Sbjct: 361 PLTF--DEIDQLVYLKAALSETLRLYPSVPEDSKFVVANDVLPDGTFVPSGSNVTYSIYS 420

Query: 487 MGRMEAIWGKDCREFKPERWMRDGRFMSESAYRFTAFNGGPRLCLGKDFAYYQMKYVAAS 546
           +GRM+ IWG+DC EFKPERW+ + R    + Y+F AFN GPR+CLGKD AY QMK + AS
Sbjct: 421 VGRMKFIWGEDCLEFKPERWLEESRDEKCNQYKFVAFNAGPRICLGKDLAYLQMKSITAS 480

Query: 547 IMSRYRVAVVTGHSVEPKLALTFYMKNGLKVNLIKRE 578
           I+ R+R+ V  GH VE K++LT +MK GLK+++ KR+
Sbjct: 481 ILLRHRLTVAPGHRVEQKMSLTLFMKFGLKMDVHKRD 508

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
XP_008461361.19.0e-31091.17PREDICTED: LOW QUALITY PROTEIN: cytochrome P450 86B1-like [Cucumis melo][more]
XP_004135954.34.9e-30789.98cytochrome P450 86B1 [Cucumis sativus] >KAE8646384.1 hypothetical protein Csa_01... [more]
XP_022954102.18.4e-29990.97cytochrome P450 86B1-like [Cucurbita moschata][more]
XP_023549182.18.4e-29990.62cytochrome P450 86B1-like [Cucurbita pepo subsp. pepo][more]
XP_038899430.11.9e-29891.50cytochrome P450 86B1-like [Benincasa hispida][more]
Match NameE-valueIdentityDescription
Q9FMY19.0e-21969.63Cytochrome P450 86B1 OS=Arabidopsis thaliana OX=3702 GN=CYP86B1 PE=2 SV=1[more]
Q9CAD61.9e-13147.78Cytochrome P450 86A7 OS=Arabidopsis thaliana OX=3702 GN=CYP86A7 PE=2 SV=1[more]
B3RFJ64.3e-12850.10Cytochrome P450 86A22 OS=Petunia hybrida OX=4102 GN=CYP86A22 PE=1 SV=1[more]
Q9LMM17.3e-12848.69Cytochrome P450 86A4 OS=Arabidopsis thaliana OX=3702 GN=CYP86A4 PE=1 SV=1[more]
O230661.2e-12748.37Cytochrome P450 86A2 OS=Arabidopsis thaliana OX=3702 GN=CYP86A2 PE=1 SV=1[more]
Match NameE-valueIdentityDescription
A0A1S3CEY84.4e-31091.17LOW QUALITY PROTEIN: cytochrome P450 86B1-like OS=Cucumis melo OX=3656 GN=LOC103... [more]
A0A6J1GQ364.1e-29990.97cytochrome P450 86B1-like OS=Cucurbita moschata OX=3662 GN=LOC111456469 PE=3 SV=... [more]
A0A6J1DJX81.2e-29589.73cytochrome P450 86B1 OS=Momordica charantia OX=3673 GN=LOC111021175 PE=3 SV=1[more]
A0A5A7UTT45.2e-29492.38Cytochrome P450 86B1-like OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffo... [more]
A0A6J1JMG12.0e-29389.91cytochrome P450 86B1-like OS=Cucurbita maxima OX=3661 GN=LOC111488206 PE=3 SV=1[more]
Match NameE-valueIdentityDescription
AT5G23190.16.4e-22069.63cytochrome P450, family 86, subfamily B, polypeptide 1 [more]
AT5G08250.11.4e-20670.18Cytochrome P450 superfamily protein [more]
AT1G24540.12.0e-14147.00cytochrome P450, family 86, subfamily C, polypeptide 1 [more]
AT1G13140.11.2e-13344.58cytochrome P450, family 86, subfamily C, polypeptide 3 [more]
AT1G63710.11.3e-13247.78cytochrome P450, family 86, subfamily A, polypeptide 7 [more]
InterPro
Analysis Name: InterPro Annotations of Watermelon (97103) v2.5
Date Performed: 2022-01-31
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR002401Cytochrome P450, E-class, group IPRINTSPR00463EP450Icoord: 361..378
score: 28.15
coord: 436..454
score: 30.58
coord: 514..524
score: 42.0
coord: 90..109
score: 20.04
coord: 381..407
score: 33.15
coord: 524..547
score: 19.39
IPR001128Cytochrome P450PRINTSPR00385P450coord: 372..389
score: 38.3
coord: 524..535
score: 35.08
coord: 515..524
score: 46.03
coord: 437..448
score: 44.94
IPR001128Cytochrome P450PFAMPF00067p450coord: 96..561
e-value: 7.4E-76
score: 255.8
IPR036396Cytochrome P450 superfamilyGENE3D1.10.630.10Cytochrome P450coord: 93..586
e-value: 1.4E-104
score: 352.1
IPR036396Cytochrome P450 superfamilySUPERFAMILY48264Cytochrome P450coord: 94..576
NoneNo IPR availablePANTHERPTHR24296CYTOCHROME P450coord: 59..577
NoneNo IPR availablePANTHERPTHR24296:SF177CYTOCHROME P450 86B1-LIKEcoord: 59..577
IPR017972Cytochrome P450, conserved sitePROSITEPS00086CYTOCHROME_P450coord: 517..526

Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
Cla97C09G166850.1Cla97C09G166850.1mRNA


GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
cellular_component GO:0016021 integral component of membrane
molecular_function GO:0020037 heme binding
molecular_function GO:0005506 iron ion binding
molecular_function GO:0004497 monooxygenase activity
molecular_function GO:0016705 oxidoreductase activity, acting on paired donors, with incorporation or reduction of molecular oxygen