Cla97C09G166530 (gene) Watermelon (97103) v2.5

Overview
NameCla97C09G166530
Typegene
OrganismCitrullus lanatus subsp. vulgaris cv. 97103 (Watermelon (97103) v2.5)
DescriptionRING-type E3 ubiquitin transferase
LocationCla97Chr09: 3592841 .. 3597888 (+)
RNA-Seq ExpressionCla97C09G166530
SyntenyCla97C09G166530
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: exonfive_prime_UTRCDSpolypeptidethree_prime_UTR
Hold the cursor over a type above to highlight its positions in the sequence below.
AATACATTCATGATTCATCCCAAACTTGAGTATTATGACAACAATACCCTCGAATTTCTGAAACACTGTCCCATTTGGCCGTAGGGGCATTTCCGGAGTTTACCCCTAAAATGAATGGCCAATGCTTATAATGGATACTGACCGCCAAAAAAGCCCTAGGTACAGCCGTACAGGCGCGAGCCATTGCTTCTTTTCCTTCTCCGAATGAATTATGAACTGCGTTGATTTCGTTCTTTTTTCCACTTTTCATTTCCCTCCACTGCTTTTATCTTATTCAAATCATCTCTTACTTTCTTTAATCAATCAAGATTTCCATTCATTTCTTTTGATTTGAATCCGTTTCTCTCCTTTCCTCGCTTTTACTTTCGATCGCCATGTCTAAAAGGTCAGTTTTCGTTTTGATTACGCTTCTTTATATGTTCTTCTTCATATTCTCACTTGTTTTTGTATGAGTATTGATGATTGGATGATGGATTAGGATTATGCTCTCCCATCATCGCTTCTTGATATTGTTTTTTTTCTTTTCTTTCTTTCTTGCGGTTCATGTTTTGCCTTGTTTGGCTGTGAAACTTAGTCGAGTTATCTTCGTCATGGTTCTTTGGTACATGGTTTAATTTATTAGGTATAGGCTATTTCGATATTTGGAATTTTAGTTTCTCGAAGGCTGTTTTGATGGGTTGTTGTTATTGATGCTGTATTTTAATATTATGAAGTTTGGGTTGTTGTTGTTTTTCATTCATGCGCGTGGTAACTTTACTTTGGTGGTTTGTTTAGGGTCCTATGCAAGTATTTTGCGCATGGAGCATGTTTAAAAGGGGAATATTGTGAGTTTTCTCATGACTGGACAGCACCACCAAGCAATGTAAATATTATTTCCACTCCTCTGTATTACCAATTATTATAATGTAAGCCAACAGTCAGTTTGGTGCTCCGACTATTCTTTCTTTCTGATGTATCGTCCATAACAGGTTTGTACTTACTATCAAAAGGGAAGGTGTGCTTTTGGCAGTCGTTGCAGATATGATCATGTTAAAGTTTCTCACGCAGAATCATCTTCGAATTCTCAGCAATCTCATGTGTCTGAATCTGTTTCTATAGCTCATCCCCAACTATCAAGGCCACTTTCTCCTTTTCCTGGATCTACAAGCGAGCTTGCTGGTTCAGAGATACCCAGTCTTCCCTTGCGTATTGAATCTGCTTGGGGTGGAGAGTCTGAACATCCAGAGAGTCTTGATGATTGGGGAAATGGGGAGGCAAGGGTTGTTGGTCCAGCTGATATACCTATCTGTTCTTTTGCCGCGGCTGGTAGTGAATGTCCACGTGGTGAAAATTGTCCTCATATTCATGGGGACCTATGTCCCACATGTGGAAAACAATGTTTACATCCTTTTAGGACTGACGAAAGAGAGGAGCACATGAAGGCATGTGAGAAAAAGCAAAAACATCTCGAGTCTTTGAAAAATAGTCAAGACATAGAGTGTAGTGTCTGCCTGGAGCGTGTTCTTTCTAAAACCGAAATAGCTGAGAGGAAATTTGGGCTTCTATCAGAATGTGATCATCCATTCTGCATTTCTTGTATTAGAAACTGGCGCAATAGTTCATCTACCTCTGGAATGGATGTTAATAGTGCTTTAAGAGCCTGCCCAATTTGTAGGAAGTTGTCATACTTTGTGATTCCAAGTGTCATTTGGTATTCTTCGAAGGAAGAAAAGCAAGAGATTGTTGATAGCTACAAGTCAAGGCTGCGGTAAATTTATGTTCTTCATTCCTAATATGTGATTTCATTCTGGAAAGTGTTCAATTGTTTTCCATTCTGCTCATGTTTTCTGCTTCCCTTTCGTTTTGTTTTTTGTTTCTTTTATATCAAACTCAATTTTGTCAGTGGAAATTATTATGTATTAAATGTAATCTGAAAAGCTCCATAGACCATAGGAATGATCAATCAAAGTTATTTAGAGAATGGAAATTGCATAACTGCTAAAGTAATTGTATGAGCTATTATTTGAGATAATTTTGCTACTCTGATCTGCGAGTTTATATTATTTTCATGTGCAGATCAATAGATTGCAAGCACTTTAACTTTGGAAATGGGAACTGCCCTTTTGGATCCAGTTGTTTTTACAAGGTAAGCTTACCTCTGAAAGCATTTACTTCAGTTTCCTTATCCTAATTGTGCTCTTCCAAGCATGCTGGATGTTGTTGTAGTGGCCTTTTATGCTGTGAGGAATTTGCAATTTTCCTGAATTTTATTCTTCTTCCTTCTGTTTTCATTATGCCTTTGTCCAAATTGTCTTCCCTTTTATTCGCCTTCCAAATTGATCATTGATATGCAGCCTGCCGAATGGAAATCTCAACCTGTTTGGTTTTGCTAATCTGTGAATGCCCTAGGAAACACGTTGAATAGTTACATGTTTGGCTTTGTGAACATGAGTTTATGAAGAAATTCTTTTTGAGATCTGGATATGTGCTGACTACTTTGGCAGTCTAAGTTCAATTCCTTGCAGGATGAAATTATATATTTTGGACACCGAGATCGTATTGCTAGACCTGCTACAATTGATTGAGGGATCCCTCTGATTGAAATTAATAAAAAGTTATATACTTACAGCCTTACAGGTGTTTGATTTGAAAGGGTTATCATGGGAAGATAATTGTAGGTTAGCACTGTAGCAAGACAGGCGGTACCTCTTCTAATCTGCGTTCTGTTAATCTATATATGCTTGCCATGCGCCAATCATCTGCGCTCGTCTTTTAGAAAGTACATATGCAACTGCTCTGTCACATTCTTCTTCATCCTATGAGTGTTGCAAGCTCAACGACCCTTGTATTTTTTGGTGACATGTCGTAGGTGTCGCATAAGCAGTGCTGTTCATTTTCAATCAGTTTTTGTGGTAGATATTGAAAGATGTGATTCCTGATGGATGTTATCTTCAGATCTATGTGCATTCTGTTTGTCATTTAATTTCGTTAGAGCAGATTGGCTTTCCAATCTTTTGATTGATACAATGTTTGTTGTGTTCCAGCATTTGGTCATTCCTGGTTCATACACATGGAAATATCACAGGCCACCTCCACGCCGACCACAATCATGGAGAACTCCTCCAAGCAGATCTAATATTGTCGATATGGATGCCTTTTTTGATATGTTCGAAGACTTAGAGGATTTGGATTTTAGTGATGAAGATTTAGATATTGAAGATTTAACACTTTTGGAGATGGCACTAATGCAGTTGGGTTGTGAAGCTAACTTATCAAATGATGAAGAGTCATAGTTTTTGAGCTACATTCTTCTTAGGTAGTTGTAACCTGATGTATATTGGGTGTTTGGCGAGTTCCTTTCTAGTCTTGTCCAAAAACTTTGAATATTTGCTACTCTAGCATTTTTGGCCACCTAGCAGTGTTCCCATGTGCAGTAAGACAAGCTCGTAGACTTTTTCAATTGTCATAATTGCCATGCTATTGGGGAGCGATACTTTGTGAAAGGCAATAGTTATTTGTCCTGGGGCAAATTAGTTACCGAGTTACCAAGATGACGACGGTCTATTTATGGAAGAACCTTTTCTTTGTGTAGATTATATTATGACTTCTTAAGTGTTGTATTTTACTACTGGGAATTAGGAATATTTGTTACCGGCTATCTTCTTCATTCCTAGATTTTACGCAGAGTTCCATTTCTTCACCCTCTTGCTTACCCACACCAAGCATGCTCTCCTAATGTTTGATTTGTGCATTTAGCAAAGTTCTGTGCAGGCTGATTCTTACCAGATTGGTTGTTTATTATCCGAGTATGTCTGTAATTTGAAGTGAATGTTCGATTAATATGTTCTATCATCTGGAAGTACTTTCCTCATATGTGCACTTTCGCACATGACTTCATCTGTTTCCAAATGGGAAGTCTATTTTTTGTTTCGAATATTTGGCTTCTTTTCTTTCATCCTTATATGCATCTGCTTTGTACTCTGCTGTATTAACTTCAAAAGGAGAGTCTTTACTAAAAAAACTTCAAAATGAGAGTCAAATTTTCATGTGATTTTCTCACTGCAGCATGCTCATCGGGATGGCCGGTTGGAAGAGATAGCTCTGCGTCATATTGGGAATGAAGATGGTCTCACTGTTATAGCTAAAAATATTAGGTTTTATTCTGTGCATTTGCTTTTTATTGTCCATTTCTTGTGAATCTTTACCTTTAAATAAATTTGAAGCTATTTTCAGGTTGTCGGATTTTCTCAGTGACTTGCATATAAGGTGAAGAACCAATTCATTCAGGTATGGAACACACCAAGCACTCTTCGCCCTTAAGCTCAGCTTCACTACATTTTGCTGTCTACTACTTGACATTTCACGTTTTAATATGGTAATCATTTGGCTTTTTTGGATGGGGGAAAGCAATTAACCTTGAAACTTATATTGCTGGTCGTTGTCATCGTTCATAGAGTCTTGGGATTTCGAAATTCAGTATCTTTATCAATTGAAACCCTACATATTCATTAGAAAATCACTTTATTTCAACACTTGGACCCTGGCTCAAAGAGTCCATGAAATGCCAAACTTTATGTATTGTTTGTCCTCAGTTACAATTGCAATGTCATCTAATAGTTAGAAACTGAAATTAACAATTTAGTCCATGTAATGTGAATAATTTTTATATTGTTAGTCTTGATATACACCTGCAATGTAATCGAAGAGTTTGAAAACCCGTTTCAGATGTCGAATTGCTTGGTGTGGAGTCTGATATTATTGAGAAATGGAAGGGATGGGGTATCGAACACGGTGCTTCAAACTTCTCATGTTCTACAAATCAAATAAAGCTAATGACATTATGTCATAGTGAGAAAATGAATGTTGCAAATCCAAGCTTATTCTTCCGATGATGGTGAAATAAAAAGGTGTTATCACATTCACATCTCACATGTATCACAGCTGAATAAAATGTACGGGTTCTTCTTATACGTGCTGTACTAAACAATTGGAAAATGACAAAATGTATTGGTTTGGTATTTTAAAAGGAAGATTAATCGGAATGTCAATGTCGTGTCTGGTATTGATAC

mRNA sequence

AATACATTCATGATTCATCCCAAACTTGAGTATTATGACAACAATACCCTCGAATTTCTGAAACACTGTCCCATTTGGCCGTAGGGGCATTTCCGGAGTTTACCCCTAAAATGAATGGCCAATGCTTATAATGGATACTGACCGCCAAAAAAGCCCTAGGTACAGCCGTACAGGCGCGAGCCATTGCTTCTTTTCCTTCTCCGAATGAATTATGAACTGCGTTGATTTCGTTCTTTTTTCCACTTTTCATTTCCCTCCACTGCTTTTATCTTATTCAAATCATCTCTTACTTTCTTTAATCAATCAAGATTTCCATTCATTTCTTTTGATTTGAATCCGTTTCTCTCCTTTCCTCGCTTTTACTTTCGATCGCCATGTCTAAAAGGGTCCTATGCAAGTATTTTGCGCATGGAGCATGTTTAAAAGGGGAATATTGTGAGTTTTCTCATGACTGGACAGCACCACCAAGCAATGTTTGTACTTACTATCAAAAGGGAAGGTGTGCTTTTGGCAGTCGTTGCAGATATGATCATGTTAAAGTTTCTCACGCAGAATCATCTTCGAATTCTCAGCAATCTCATGTGTCTGAATCTGTTTCTATAGCTCATCCCCAACTATCAAGGCCACTTTCTCCTTTTCCTGGATCTACAAGCGAGCTTGCTGGTTCAGAGATACCCAGTCTTCCCTTGCGTATTGAATCTGCTTGGGGTGGAGAGTCTGAACATCCAGAGAGTCTTGATGATTGGGGAAATGGGGAGGCAAGGGTTGTTGGTCCAGCTGATATACCTATCTGTTCTTTTGCCGCGGCTGGTAGTGAATGTCCACGTGGTGAAAATTGTCCTCATATTCATGGGGACCTATGTCCCACATGTGGAAAACAATGTTTACATCCTTTTAGGACTGACGAAAGAGAGGAGCACATGAAGGCATGTGAGAAAAAGCAAAAACATCTCGAGTCTTTGAAAAATAGTCAAGACATAGAGTGTAGTGTCTGCCTGGAGCGTGTTCTTTCTAAAACCGAAATAGCTGAGAGGAAATTTGGGCTTCTATCAGAATGTGATCATCCATTCTGCATTTCTTGTATTAGAAACTGGCGCAATAGTTCATCTACCTCTGGAATGGATGTTAATAGTGCTTTAAGAGCCTGCCCAATTTGTAGGAAGTTGTCATACTTTGTGATTCCAAGTGTCATTTGGTATTCTTCGAAGGAAGAAAAGCAAGAGATTGTTGATAGCTACAAGTCAAGGCTGCGATCAATAGATTGCAAGCACTTTAACTTTGGAAATGGGAACTGCCCTTTTGGATCCAGTTGTTTTTACAAGCATTTGGTCATTCCTGGTTCATACACATGGAAATATCACAGGCCACCTCCACGCCGACCACAATCATGGAGAACTCCTCCAAGCAGATCTAATATTGTCGATATGGATGCCTTTTTTGATATGTTCGAAGACTTAGAGGATTTGGATTTTAGTGATGAAGATTTAGATATTGAAGATTTAACACTTTTGGAGATGGCACTAATGCAGTTGGGTTGTGAAGCTAACTTATCAAATGATGAAGAGTCATAGTTTTTGAGCTACATTCTTCTTAGGTAGTTGTAACCTGATGTATATTGGGTGTTTGGCGAGTTCCTTTCTAGTCTTGTCCAAAAACTTTGAATATTTGCTACTCTAGCATTTTTGGCCACCTAGCAGTGTTCCCATGTGCAGTAAGACAAGCTCGTAGACTTTTTCAATTGTCATAATTGCCATGCTATTGGGGAGCGATACTTTGTGAAAGGCAATAGTTATTTGTCCTGGGGCAAATTAGTTACCGAGTTACCAAGATGACGACGGTCTATTTATGGAAGAACCTTTTCTTTGTGTAGATTATATTATGACTTCTTAAGTGTTGTATTTTACTACTGGGAATTAGGAATATTTGTTACCGGCTATCTTCTTCATTCCTAGATTTTACGCAGAGTTCCATTTCTTCACCCTCTTGCTTACCCACACCAAGCATGCTCTCCTAATGTTTGATTTGTGCATTTAGCAAAGTTCTGTGCAGGCTGATTCTTACCAGATTGGTTGTTTATTATCCGAGTATGTCTGTAATTTGAAGTGAATGTTCGATTAATATGTTCTATCATCTGGAAGTACTTTCCTCATATGTGCACTTTCGCACATGACTTCATCTGTTTCCAAATGGGAAGTCTATTTTTTGTTTCGAATATTTGGCTTCTTTTCTTTCATCCTTATATGCATCTGCTTTGTACTCTGCTGTATTAACTTCAAAAGGAGAGTCTTTACTAAAAAAACTTCAAAATGAGAGTCAAATTTTCATGTGATTTTCTCACTGCAGCATGCTCATCGGGATGGCCGGTTGGAAGAGATAGCTCTGCGTCATATTGGGAATGAAGATGGTCTCACTGTTATAGCTAAAAATATTAGGTTGTCGGATTTTCTCAGTGACTTGCATATAAGGTGAAGAACCAATTCATTCAGATGTCGAATTGCTTGGTGTGGAGTCTGATATTATTGAGAAATGGAAGGGATGGGGTATCGAACACGGTGCTTCAAACTTCTCATGTTCTACAAATCAAATAAAGCTAATGACATTATGTCATAGTGAGAAAATGAATGTTGCAAATCCAAGCTTATTCTTCCGATGATGGTGAAATAAAAAGGTGTTATCACATTCACATCTCACATGTATCACAGCTGAATAAAATGTACGGGTTCTTCTTATACGTGCTGTACTAAACAATTGGAAAATGACAAAATGTATTGGTTTGGTATTTTAAAAGGAAGATTAATCGGAATGTCAATGTCGTGTCTGGTATTGATAC

Coding sequence (CDS)

ATGTCTAAAAGGGTCCTATGCAAGTATTTTGCGCATGGAGCATGTTTAAAAGGGGAATATTGTGAGTTTTCTCATGACTGGACAGCACCACCAAGCAATGTTTGTACTTACTATCAAAAGGGAAGGTGTGCTTTTGGCAGTCGTTGCAGATATGATCATGTTAAAGTTTCTCACGCAGAATCATCTTCGAATTCTCAGCAATCTCATGTGTCTGAATCTGTTTCTATAGCTCATCCCCAACTATCAAGGCCACTTTCTCCTTTTCCTGGATCTACAAGCGAGCTTGCTGGTTCAGAGATACCCAGTCTTCCCTTGCGTATTGAATCTGCTTGGGGTGGAGAGTCTGAACATCCAGAGAGTCTTGATGATTGGGGAAATGGGGAGGCAAGGGTTGTTGGTCCAGCTGATATACCTATCTGTTCTTTTGCCGCGGCTGGTAGTGAATGTCCACGTGGTGAAAATTGTCCTCATATTCATGGGGACCTATGTCCCACATGTGGAAAACAATGTTTACATCCTTTTAGGACTGACGAAAGAGAGGAGCACATGAAGGCATGTGAGAAAAAGCAAAAACATCTCGAGTCTTTGAAAAATAGTCAAGACATAGAGTGTAGTGTCTGCCTGGAGCGTGTTCTTTCTAAAACCGAAATAGCTGAGAGGAAATTTGGGCTTCTATCAGAATGTGATCATCCATTCTGCATTTCTTGTATTAGAAACTGGCGCAATAGTTCATCTACCTCTGGAATGGATGTTAATAGTGCTTTAAGAGCCTGCCCAATTTGTAGGAAGTTGTCATACTTTGTGATTCCAAGTGTCATTTGGTATTCTTCGAAGGAAGAAAAGCAAGAGATTGTTGATAGCTACAAGTCAAGGCTGCGATCAATAGATTGCAAGCACTTTAACTTTGGAAATGGGAACTGCCCTTTTGGATCCAGTTGTTTTTACAAGCATTTGGTCATTCCTGGTTCATACACATGGAAATATCACAGGCCACCTCCACGCCGACCACAATCATGGAGAACTCCTCCAAGCAGATCTAATATTGTCGATATGGATGCCTTTTTTGATATGTTCGAAGACTTAGAGGATTTGGATTTTAGTGATGAAGATTTAGATATTGAAGATTTAACACTTTTGGAGATGGCACTAATGCAGTTGGGTTGTGAAGCTAACTTATCAAATGATGAAGAGTCATAG

Protein sequence

MSKRVLCKYFAHGACLKGEYCEFSHDWTAPPSNVCTYYQKGRCAFGSRCRYDHVKVSHAESSSNSQQSHVSESVSIAHPQLSRPLSPFPGSTSELAGSEIPSLPLRIESAWGGESEHPESLDDWGNGEARVVGPADIPICSFAAAGSECPRGENCPHIHGDLCPTCGKQCLHPFRTDEREEHMKACEKKQKHLESLKNSQDIECSVCLERVLSKTEIAERKFGLLSECDHPFCISCIRNWRNSSSTSGMDVNSALRACPICRKLSYFVIPSVIWYSSKEEKQEIVDSYKSRLRSIDCKHFNFGNGNCPFGSSCFYKHLVIPGSYTWKYHRPPPRRPQSWRTPPSRSNIVDMDAFFDMFEDLEDLDFSDEDLDIEDLTLLEMALMQLGCEANLSNDEES
Homology
BLAST of Cla97C09G166530 vs. NCBI nr
Match: XP_038898014.1 (E3 ubiquitin-protein ligase makorin isoform X1 [Benincasa hispida] >XP_038898015.1 E3 ubiquitin-protein ligase makorin isoform X1 [Benincasa hispida])

HSP 1 Score: 805.1 bits (2078), Expect = 2.8e-229
Identity = 379/398 (95.23%), Postives = 387/398 (97.24%), Query Frame = 0

Query: 1   MSKRVLCKYFAHGACLKGEYCEFSHDWTAPPSNVCTYYQKGRCAFGSRCRYDHVKVSHAE 60
           MSKRVLCKYFAHGACLKGEYCEFSHDWTAPPSNVCTYYQKGRCAFGSRCRYDHVKVSHAE
Sbjct: 1   MSKRVLCKYFAHGACLKGEYCEFSHDWTAPPSNVCTYYQKGRCAFGSRCRYDHVKVSHAE 60

Query: 61  SSSNSQQSHVSESVSIAHPQLSRPLSPFPGSTSELAGSEIPSLPLRIESAWGGESEHPES 120
           SSSNSQQSHVSESVSIAHP LS PLSPFPGSTSELAGSEIPSLPLR+E+AW GESE P+ 
Sbjct: 61  SSSNSQQSHVSESVSIAHPHLSSPLSPFPGSTSELAGSEIPSLPLRVENAWSGESERPDG 120

Query: 121 LDDWGNGEARVVGPADIPICSFAAAGSECPRGENCPHIHGDLCPTCGKQCLHPFRTDERE 180
           LDDWGNGEAR++ PADIPICSFAAAGSECPRGENCPHIHGDLCPTCGKQCLHP+RTDERE
Sbjct: 121 LDDWGNGEARIIHPADIPICSFAAAGSECPRGENCPHIHGDLCPTCGKQCLHPYRTDERE 180

Query: 181 EHMKACEKKQKHLESLKNSQDIECSVCLERVLSKTEIAERKFGLLSECDHPFCISCIRNW 240
           EHMKACEKKQKHLESLK+SQDIECSVCLERVLSKTEIAERKFGLLSECDHPFCISCIRNW
Sbjct: 181 EHMKACEKKQKHLESLKHSQDIECSVCLERVLSKTEIAERKFGLLSECDHPFCISCIRNW 240

Query: 241 RNSSSTSGMDVNSALRACPICRKLSYFVIPSVIWYSSKEEKQEIVDSYKSRLRSIDCKHF 300
           RNSSSTSGMDVNSALRACPICRKLSYFVIPSVIWYSSKEEKQEIVDSYKSRLRSIDCKHF
Sbjct: 241 RNSSSTSGMDVNSALRACPICRKLSYFVIPSVIWYSSKEEKQEIVDSYKSRLRSIDCKHF 300

Query: 301 NFGNGNCPFGSSCFYKHLVIPGSYTWKYHRPPPRRPQSWRTPPSRSNIVDMDAFFDMFED 360
           NFGNGNCPFGSSCFYKHLVIPGSYTWKYHRPPPRRP SWRTPPSRSNIVDMDAFFDMFED
Sbjct: 301 NFGNGNCPFGSSCFYKHLVIPGSYTWKYHRPPPRRPHSWRTPPSRSNIVDMDAFFDMFED 360

Query: 361 LEDLDFSDEDLDIEDLTLLEMALMQLGCEANLSNDEES 399
           LEDLDFS EDLD EDLTLLEMALMQL CEAN S+DEES
Sbjct: 361 LEDLDFSGEDLDFEDLTLLEMALMQLSCEANSSSDEES 398

BLAST of Cla97C09G166530 vs. NCBI nr
Match: XP_038898016.1 (E3 ubiquitin-protein ligase makorin isoform X2 [Benincasa hispida])

HSP 1 Score: 784.3 bits (2024), Expect = 5.1e-223
Identity = 368/386 (95.34%), Postives = 376/386 (97.41%), Query Frame = 0

Query: 1   MSKRVLCKYFAHGACLKGEYCEFSHDWTAPPSNVCTYYQKGRCAFGSRCRYDHVKVSHAE 60
           MSKRVLCKYFAHGACLKGEYCEFSHDWTAPPSNVCTYYQKGRCAFGSRCRYDHVKVSHAE
Sbjct: 1   MSKRVLCKYFAHGACLKGEYCEFSHDWTAPPSNVCTYYQKGRCAFGSRCRYDHVKVSHAE 60

Query: 61  SSSNSQQSHVSESVSIAHPQLSRPLSPFPGSTSELAGSEIPSLPLRIESAWGGESEHPES 120
           SSSNSQQSHVSESVSIAHP LS PLSPFPGSTSELAGSEIPSLPLR+E+AW GESE P+ 
Sbjct: 61  SSSNSQQSHVSESVSIAHPHLSSPLSPFPGSTSELAGSEIPSLPLRVENAWSGESERPDG 120

Query: 121 LDDWGNGEARVVGPADIPICSFAAAGSECPRGENCPHIHGDLCPTCGKQCLHPFRTDERE 180
           LDDWGNGEAR++ PADIPICSFAAAGSECPRGENCPHIHGDLCPTCGKQCLHP+RTDERE
Sbjct: 121 LDDWGNGEARIIHPADIPICSFAAAGSECPRGENCPHIHGDLCPTCGKQCLHPYRTDERE 180

Query: 181 EHMKACEKKQKHLESLKNSQDIECSVCLERVLSKTEIAERKFGLLSECDHPFCISCIRNW 240
           EHMKACEKKQKHLESLK+SQDIECSVCLERVLSKTEIAERKFGLLSECDHPFCISCIRNW
Sbjct: 181 EHMKACEKKQKHLESLKHSQDIECSVCLERVLSKTEIAERKFGLLSECDHPFCISCIRNW 240

Query: 241 RNSSSTSGMDVNSALRACPICRKLSYFVIPSVIWYSSKEEKQEIVDSYKSRLRSIDCKHF 300
           RNSSSTSGMDVNSALRACPICRKLSYFVIPSVIWYSSKEEKQEIVDSYKSRLRSIDCKHF
Sbjct: 241 RNSSSTSGMDVNSALRACPICRKLSYFVIPSVIWYSSKEEKQEIVDSYKSRLRSIDCKHF 300

Query: 301 NFGNGNCPFGSSCFYKHLVIPGSYTWKYHRPPPRRPQSWRTPPSRSNIVDMDAFFDMFED 360
           NFGNGNCPFGSSCFYKHLVIPGSYTWKYHRPPPRRP SWRTPPSRSNIVDMDAFFDMFED
Sbjct: 301 NFGNGNCPFGSSCFYKHLVIPGSYTWKYHRPPPRRPHSWRTPPSRSNIVDMDAFFDMFED 360

Query: 361 LEDLDFSDEDLDIEDLTLLEMALMQL 387
           LEDLDFS EDLD EDLTLLEMALM +
Sbjct: 361 LEDLDFSGEDLDFEDLTLLEMALMHM 386

BLAST of Cla97C09G166530 vs. NCBI nr
Match: XP_031744426.1 (E3 ubiquitin-protein ligase makorin isoform X1 [Cucumis sativus] >XP_031744427.1 E3 ubiquitin-protein ligase makorin isoform X1 [Cucumis sativus] >KGN45141.1 hypothetical protein Csa_015725 [Cucumis sativus])

HSP 1 Score: 781.2 bits (2016), Expect = 4.3e-222
Identity = 369/398 (92.71%), Postives = 380/398 (95.48%), Query Frame = 0

Query: 1   MSKRVLCKYFAHGACLKGEYCEFSHDWTAPPSNVCTYYQKGRCAFGSRCRYDHVKVSHAE 60
           MSKRVLCKYFAHGACLKGEYCEFSHDWTAPPSNVCTYYQKGRCAFGSRCRYDHVKVSHAE
Sbjct: 1   MSKRVLCKYFAHGACLKGEYCEFSHDWTAPPSNVCTYYQKGRCAFGSRCRYDHVKVSHAE 60

Query: 61  SSSNSQQSHVSESVSIAHPQLSRPLSPFPGSTSELAGSEIPSLPLRIESAWGGESEHPES 120
           S SN QQSH SESVS+AH QLSRPLSPF GSTSELA SEIPSLPLR E+AW GE   P+ 
Sbjct: 61  SCSNVQQSHGSESVSVAHSQLSRPLSPFSGSTSELASSEIPSLPLREEAAWSGEFVRPDG 120

Query: 121 LDDWGNGEARVVGPADIPICSFAAAGSECPRGENCPHIHGDLCPTCGKQCLHPFRTDERE 180
           LDDW NGEARVVGPADIPICSFAAAG +CPRGENCPHIHGDLCPTCGKQCLHPFRTDERE
Sbjct: 121 LDDWVNGEARVVGPADIPICSFAAAGGDCPRGENCPHIHGDLCPTCGKQCLHPFRTDERE 180

Query: 181 EHMKACEKKQKHLESLKNSQDIECSVCLERVLSKTEIAERKFGLLSECDHPFCISCIRNW 240
           EH+KACEKKQKHLESLK+SQDIECSVCLERVLSKTE AERKFG+LSECDHPFCISCIRNW
Sbjct: 181 EHLKACEKKQKHLESLKHSQDIECSVCLERVLSKTETAERKFGILSECDHPFCISCIRNW 240

Query: 241 RNSSSTSGMDVNSALRACPICRKLSYFVIPSVIWYSSKEEKQEIVDSYKSRLRSIDCKHF 300
           RNSSSTSGMD+NSALRACPICRKLSYFVIPSVIWYSSKEEKQEIVDSYKSRLRSIDCKHF
Sbjct: 241 RNSSSTSGMDINSALRACPICRKLSYFVIPSVIWYSSKEEKQEIVDSYKSRLRSIDCKHF 300

Query: 301 NFGNGNCPFGSSCFYKHLVIPGSYTWKYHRPPPRRPQSWRTPPSRSNIVDMDAFFDMFED 360
           NFGNGNCPFGSSCFYKHLVIPGSYTWKYHRPPPR+PQSWRTPPSRSNIVDMDAFF+MFED
Sbjct: 301 NFGNGNCPFGSSCFYKHLVIPGSYTWKYHRPPPRQPQSWRTPPSRSNIVDMDAFFEMFED 360

Query: 361 LEDLDFSDEDLDIEDLTLLEMALMQLGCEANLSNDEES 399
           LEDLDFSDEDLD E LTLLEMALMQLGCEAN S+DEES
Sbjct: 361 LEDLDFSDEDLDFEYLTLLEMALMQLGCEANSSSDEES 398

BLAST of Cla97C09G166530 vs. NCBI nr
Match: KAA0058639.1 (E3 ubiquitin-protein ligase makorin [Cucumis melo var. makuwa] >TYK10446.1 E3 ubiquitin-protein ligase makorin [Cucumis melo var. makuwa])

HSP 1 Score: 755.4 bits (1949), Expect = 2.5e-214
Identity = 368/450 (81.78%), Postives = 379/450 (84.22%), Query Frame = 0

Query: 1   MSKRVLCKYFAHGACLKGEYCEFSHDWTAPPSNVCTYYQKGRCAFGSRCRYDHVKVSHAE 60
           MSKRVLCKYFAHGACLKGEYCEFSHDWTAPPSNVCTYYQKGRCAFGSRCRYDHVKVSH E
Sbjct: 1   MSKRVLCKYFAHGACLKGEYCEFSHDWTAPPSNVCTYYQKGRCAFGSRCRYDHVKVSHTE 60

Query: 61  SSSNSQQSHVSESVSIAHPQLSRPLSPFPGSTSELAGSEIPSLPLRIESAWGGESEHPES 120
           S SN QQSH SES+S+AH QLSRPLSPF GSTSELA SE+PSLPLR E+AW GE   P+ 
Sbjct: 61  SCSNVQQSHGSESLSVAHSQLSRPLSPFSGSTSELASSEMPSLPLRHETAWSGEFVRPDG 120

Query: 121 LDDWGNGEARVVGPADIPICSFAAAGSECPRGENCPHIHGDLCPTCGKQCLHPFRTDERE 180
           LDDWGNGEARVVGPADIPICSFAAAG +CPRGENCP IHGDLCPTCGKQCLHPFRTDERE
Sbjct: 121 LDDWGNGEARVVGPADIPICSFAAAGGDCPRGENCPRIHGDLCPTCGKQCLHPFRTDERE 180

Query: 181 EHMKACEKKQKHLESLKNSQDIECSVCLERVLSKTEIAERKFGLLSECDHPFCISCIRNW 240
           EH+KACEKKQKHLESLK+SQDIECSVCLERVLSKTE AERKFG+LSECDHPFCISCIRNW
Sbjct: 181 EHLKACEKKQKHLESLKHSQDIECSVCLERVLSKTETAERKFGILSECDHPFCISCIRNW 240

Query: 241 RNSSSTSGMDVNSALRACPICRKLSYFVIPSVIWYSSKEEKQEIVDSYKSRLRSIDCKHF 300
           RNSSSTSGMDVNSALRACPICRKLSYFVIPSVIWYSSKEEKQEIVDSYKSRLRSIDCKHF
Sbjct: 241 RNSSSTSGMDVNSALRACPICRKLSYFVIPSVIWYSSKEEKQEIVDSYKSRLRSIDCKHF 300

Query: 301 NFGNGNCPFGSSCFYK-------------------------------------------- 360
           NFGNGNCPFGSSCFYK                                            
Sbjct: 301 NFGNGNCPFGSSCFYKLLLSPLFDNFYGSIQVQSDVLTTLADLFAFCLSFNFIRADWLSN 360

Query: 361 --------HLVIPGSYTWKYHRPPPRRPQSWRTPPSRSNIVDMDAFFDMFEDLEDLDFSD 399
                   HLVIPGSYTWKYHRPPPRRPQSWRTPPSRSNIVDMDAFF+MFEDLEDLDFSD
Sbjct: 361 DTMFVVFQHLVIPGSYTWKYHRPPPRRPQSWRTPPSRSNIVDMDAFFEMFEDLEDLDFSD 420

BLAST of Cla97C09G166530 vs. NCBI nr
Match: XP_023547951.1 (E3 ubiquitin-protein ligase makorin-like isoform X1 [Cucurbita pepo subsp. pepo])

HSP 1 Score: 745.3 bits (1923), Expect = 2.6e-211
Identity = 358/402 (89.05%), Postives = 375/402 (93.28%), Query Frame = 0

Query: 1   MSKRVLCKYFAHGACLKGEYCEFSHDWTAPPSNVCTYYQKGRCAFGSRCRYDHVKVSHAE 60
           MSKRVLCKYF+HGACLKGEYCEFSHDW+APPSNVCTYYQKGRCAFGSRCRYDHVKVSHAE
Sbjct: 1   MSKRVLCKYFSHGACLKGEYCEFSHDWSAPPSNVCTYYQKGRCAFGSRCRYDHVKVSHAE 60

Query: 61  S--SSNSQQSHVSESVSIAHPQLSRPLSPFPGSTSELAGSEIPSLPLRIESAWGGESEHP 120
           S  SSNSQQ HVSESVSIAHP LSRPLSP PGSTSE AGS+I   P +++  W GESEHP
Sbjct: 61  SSASSNSQQPHVSESVSIAHPLLSRPLSPLPGSTSEFAGSKISCPPSQVQIVWSGESEHP 120

Query: 121 ESLDDWGNGEARVVGPADIPICSFAAAGSECPRGENCPHIHGDLCPTCGKQCLHPFRTDE 180
           + LDDW NGE R+VGPADIPICSFAAAGS+CPRG+NCPHIHGDLCPTCGKQCLHPFRTDE
Sbjct: 121 DGLDDWRNGETRIVGPADIPICSFAAAGSDCPRGQNCPHIHGDLCPTCGKQCLHPFRTDE 180

Query: 181 REEHMKACEKKQKHLESLKNSQDIECSVCLERVLSKTEIAERKFGLLSECDHPFCISCIR 240
           REEH+KACEKKQKHLESLK SQDIECSVCLERVLSKTEIAERKFGLLSECDHPFCISCIR
Sbjct: 181 REEHIKACEKKQKHLESLKRSQDIECSVCLERVLSKTEIAERKFGLLSECDHPFCISCIR 240

Query: 241 NWRNSSSTSGMDVNSALRACPICRKLSYFVIPSVIWYSSKEEKQEIVDSYKSRLRSIDCK 300
           NWRN+SSTSGMDVNSALRACPICRKLSYFVIPSVIWYSSKEEKQEIVDSYKSRLRSIDCK
Sbjct: 241 NWRNTSSTSGMDVNSALRACPICRKLSYFVIPSVIWYSSKEEKQEIVDSYKSRLRSIDCK 300

Query: 301 HFNFGNGNCPFGSSCFYKHLVIPGSYTWKYHRPPPRRPQSWRTPPSRSNIVDMDAFFDMF 360
           HFNFGNGNCPFGSSCFYKHLV PGSY+WKYHRPPPRRP+S RT P+RSNIVDMDAFFDMF
Sbjct: 301 HFNFGNGNCPFGSSCFYKHLVKPGSYSWKYHRPPPRRPRSQRT-PNRSNIVDMDAFFDMF 360

Query: 361 EDLEDLDFSDEDLDIEDLTLLEMA--LMQLGCEANLSNDEES 399
           EDLEDLDFS EDLD EDLT LEMA  LMQLG EAN S+DEE+
Sbjct: 361 EDLEDLDFSAEDLDFEDLTPLEMAFVLMQLGREANSSSDEEN 401

BLAST of Cla97C09G166530 vs. ExPASy Swiss-Prot
Match: E0X9N4 (Putative RING-type E3 ubiquitin transferase C3H69 OS=Arabidopsis thaliana OX=3702 GN=At3g63550 PE=3 SV=1)

HSP 1 Score: 430.3 bits (1105), Expect = 2.5e-119
Identity = 202/317 (63.72%), Postives = 244/317 (76.97%), Query Frame = 0

Query: 1   MSKRVLCKYFAHGACLKGEYCEFSHDWTAPPSNVCTYYQKGRCAFGSRCRYDHVKVSHAE 60
           MSKR+LCK+FAHGACLKGE+CEFSHDW  P +N+CTYYQKG C++GSRCRY+HVK S   
Sbjct: 1   MSKRILCKFFAHGACLKGEHCEFSHDWKDPTNNICTYYQKGICSYGSRCRYEHVKASRPH 60

Query: 61  SSSNSQQSHVSESVSIAHPQLSRPLSPFPGSTSELAGSEIPSLPLRIESAWGGESEHPES 120
            S++S  +    S + A   L+    P     +E   S +PS  L+    W  +S H +S
Sbjct: 61  PSASSSSAVPRPSPASASNTLACAFLP---GVAERDLSPVPSSSLK--PTWNLDSLHQDS 120

Query: 121 LDDWGNGEARVVGPADIPICSFAAAGSECPRGENCPHIHGDLCPTCGKQCLHPFRTDERE 180
           +D+        V P ++PICSFAAAG +CPRG  CPHIHG +CPTCGK CLHPFR DERE
Sbjct: 121 VDEVKTSNPGTVKPEELPICSFAAAG-DCPRGNECPHIHGTICPTCGKCCLHPFRPDERE 180

Query: 181 EHMKACEKKQKHLESLKNSQDIECSVCLERVLSKTEIAERKFGLLSECDHPFCISCIRNW 240
           EH K CEKK K LE+LK SQ+IEC VCLERVLSK   AERKFGLL+ECDH FCI+CIRNW
Sbjct: 181 EHKKVCEKKHKQLEALKLSQEIECCVCLERVLSKATPAERKFGLLTECDHAFCIACIRNW 240

Query: 241 RNSSSTSGMDVNSALRACPICRKLSYFVIPSVIWYSSKEEKQEIVDSYKSRLRSIDCKHF 300
           R+SS ++GMDVNS LRACPICRKLSYFV+PSVIW+S+ EEK+EI+D+Y+ +LRSIDCKHF
Sbjct: 241 RSSSPSTGMDVNSTLRACPICRKLSYFVVPSVIWFSAPEEKKEIMDNYREKLRSIDCKHF 300

Query: 301 NFGNGNCPFGSSCFYKH 318
           +FG+GNCPFG+SCFYKH
Sbjct: 301 SFGDGNCPFGTSCFYKH 311

BLAST of Cla97C09G166530 vs. ExPASy Swiss-Prot
Match: Q5ZA07 (E3 ubiquitin-protein ligase makorin OS=Oryza sativa subsp. japonica OX=39947 GN=MKRN PE=2 SV=1)

HSP 1 Score: 420.2 bits (1079), Expect = 2.5e-116
Identity = 208/336 (61.90%), Postives = 241/336 (71.73%), Query Frame = 0

Query: 2   SKRVLCKYFAHGACLKGEYCEFSHDWTAPPSNVCTYYQKGRCAFGSRCRYDHVKVSH--- 61
           +KRVLCK+F HGACLKGEYCEFSHDW   P+NVCT+YQKG C++GSRCRYDHVKVS    
Sbjct: 3   TKRVLCKFFMHGACLKGEYCEFSHDWNDQPNNVCTFYQKGSCSYGSRCRYDHVKVSRNPT 62

Query: 62  -AESSSNSQQSHVSESVSIAHPQLSRPLS-PFPGSTSELAGSEIPSLPLRIE-------- 121
            A   S+S  +  S S+        +PLS   P      A S  P   + ++        
Sbjct: 63  VAPPPSSSTTTRASSSL--------QPLSFGRPHHVGYQADSSNPRQQISMDVLAHSGSK 122

Query: 122 SAWGGESEHPESLD---DWGNG----EARVVGPADIPICSFAAAGSECPRGENCPHIHGD 181
             W  + +H   L+   DW           + PAD+PICSFAA G+ CP GE CP +HGD
Sbjct: 123 PVWRNDFQHESVLEDGIDWSISPTVQNQTTLSPADLPICSFAAGGN-CPYGEECPQMHGD 182

Query: 182 LCPTCGKQCLHPFRTDEREEHMKACEKKQKHLESLKNSQDIECSVCLERVLSKTEIAERK 241
           LC TCGK CLHP+R DEREEH K CEK  K LESLK SQ+IECSVCL+RVLSK   AERK
Sbjct: 183 LCTTCGKMCLHPYRPDEREEHTKLCEKNHKRLESLKRSQEIECSVCLDRVLSKPTAAERK 242

Query: 242 FGLLSECDHPFCISCIRNWRNSSSTSGMDVNSALRACPICRKLSYFVIPSVIWYSSKEEK 301
           FGLLSECDHPFCISCIRNWRN+S TSGMDVNSALRACPICRKLSY+VIPSV+WY SKEEK
Sbjct: 243 FGLLSECDHPFCISCIRNWRNNSPTSGMDVNSALRACPICRKLSYYVIPSVLWYFSKEEK 302

Query: 302 QEIVDSYKSRLRSIDCKHFNFGNGNCPFGSSCFYKH 318
            EI+D+YK++L+SIDCK+F+FG G CPFGSSCFYKH
Sbjct: 303 LEIIDNYKAKLKSIDCKYFDFGTGTCPFGSSCFYKH 329

BLAST of Cla97C09G166530 vs. ExPASy Swiss-Prot
Match: Q6IDS6 (E3 ubiquitin-protein ligase makorin OS=Arabidopsis thaliana OX=3702 GN=MKRN PE=2 SV=1)

HSP 1 Score: 410.2 bits (1053), Expect = 2.6e-113
Identity = 197/322 (61.18%), Postives = 231/322 (71.74%), Query Frame = 0

Query: 1   MSKRVLCKYFAHGACLKGEYCEFSHDWTAPPSNVCTYYQKGRCAFGSRCRYDHVKVSHAE 60
           MS R+LCK+F HG+CLKGE CEFSHD   PP+NVCT+YQK  C +GSRCRYDHV+     
Sbjct: 1   MSDRILCKFFVHGSCLKGENCEFSHDSKDPPNNVCTFYQKRICLYGSRCRYDHVR----- 60

Query: 61  SSSNSQQSHVSESVSIAHPQLSRPLSPFPGSTSELAGSEIPSLPLRIESAWGGESEHPES 120
           ++SN   S  SES       L R +S  P    +  G                     ++
Sbjct: 61  AASNLPLSSDSES-------LDRSISTTPSRHLQQQG---------------------DN 120

Query: 121 LDDWGNGEARVVGPADIPICSFAAAGSECPRGENCPHIHGDLCPTCGKQCLHPFRTDERE 180
            D   +     + P + PICSFAAAG +CPRG  CPH+HGDLC TCGK+CLHPFR +ERE
Sbjct: 121 NDGDKSSNVYCIHPREYPICSFAAAG-DCPRGNQCPHMHGDLCNTCGKKCLHPFRPEERE 180

Query: 181 EHMKACEKKQKHLESLKNSQDIECSVCLERVLSKTEIAERKFGLLSECDHPFCISCIRNW 240
           EH K CEKKQKH+E+LK SQDIECSVCL+R+LSK    ERKFGLL+ECDHPFCI CIRNW
Sbjct: 181 EHTKECEKKQKHIEALKQSQDIECSVCLDRILSKATPGERKFGLLTECDHPFCIQCIRNW 240

Query: 241 RNSSSTSGMDVNSALRACPICRKLSYFVIPSVIWYSSKEEKQEIVDSYKSRLRSIDCKHF 300
           R+S+  SGMDVNS LRACPICRKLSYFV+PSV+WYSS EEK+EI+D YK++LRSIDCKHF
Sbjct: 241 RSSAPVSGMDVNSTLRACPICRKLSYFVVPSVVWYSSPEEKKEIIDIYKAKLRSIDCKHF 288

Query: 301 NFGNGNCPFGSSCFYKHLVIPG 323
           NFGNGNCPFG+SCFYKH    G
Sbjct: 301 NFGNGNCPFGASCFYKHAYSDG 288

BLAST of Cla97C09G166530 vs. ExPASy Swiss-Prot
Match: Q8JFF3 (Probable E3 ubiquitin-protein ligase makorin-1 OS=Seriola quinqueradiata OX=8161 GN=mkrn1 PE=2 SV=1)

HSP 1 Score: 243.8 bits (621), Expect = 3.3e-63
Identity = 151/410 (36.83%), Postives = 224/410 (54.63%), Query Frame = 0

Query: 2   SKRVLCKYFAHGACLKGEYCEFSHDWT--APPSNVCTYYQKGRCAFGSRCRYDHVKVSHA 61
           +K V C+YF HG C +G+ C +SHD T   P + +C ++QKG C FG RCR++H K +  
Sbjct: 19  TKHVTCRYFMHGLCKEGDNCRYSHDLTNSKPAAMICKFFQKGNCVFGDRCRFEHCKPAKN 78

Query: 62  ESSSNSQQSHVSESVSIAHPQLSRPL--SPFPGSTSELAGSE-IPSLPL--RIESAWGGE 121
           E     Q   +  S S+A P    P   +P PG+   +  +E +P  P   R E A   E
Sbjct: 79  EELPAPQMLPL-PSASLAGPSDPEPSGPTPVPGAQDWVNAAEFVPGQPYCGRAEQA-KVE 138

Query: 122 SEHP--ESLDDWGNGEARVVGPADIPICSFAAAGSECPRGENCPHIHGDLCPTCGKQCLH 181
           S  P  E  D +   + + +      +C +AA G EC  G NC ++HGD+C  CG Q LH
Sbjct: 139 SSVPLIEEFDSYPAPDNKQLRK---QLCPYAAVG-ECRYGINCAYLHGDVCYMCGLQVLH 198

Query: 182 PFRTDEREEHMKAC-EKKQKHLE---SLKNSQDIECSVCLERVLSKTEIAERKFGLLSEC 241
           P   ++R EH KAC E  +K +E   +++ S+D+ C VC+E V  K   +ER+FG+LS C
Sbjct: 199 PTDNNQRSEHTKACIEAHEKDMEISFAIQRSKDMMCGVCMEVVFEKANPSERRFGILSNC 258

Query: 242 DHPFCISCIRNWRNSSSTSGMDVNSALRACPICRKLSYFVIPSVIWYSSKEEKQEIVDSY 301
            H +C+ CIR WR++        +  +++CP CR  S FVIPS  W   K++KQ+++  Y
Sbjct: 259 SHCYCLKCIRKWRSAKQFE----SKIIKSCPECRITSNFVIPSEYWVEDKDDKQKLIQKY 318

Query: 302 KSRLRSIDCKHFNFGNGNCPFGSSCFYKHLVIPGSYTWKYHRPPPRRPQSWRTPPSRSNI 361
           K  + S  C++F+ G G CPFGS+CFYKH    G    +     P+R Q+     SR+  
Sbjct: 319 KDGMGSKPCRYFDEGRGTCPFGSNCFYKHAFPDG----RLEEAQPQRRQTGSN--SRNRN 378

Query: 362 VDMDAFFDMFEDLEDLD-FSDEDLDIEDLTLLEMALMQLGCEANLSNDEE 398
                 +D++++ E  D F +ED ++    L EM LM L   A  ++DEE
Sbjct: 379 SRRTPLWDIYDERESTDSFDNEDEEMVTFELSEMLLMLL---AAGTDDEE 409

BLAST of Cla97C09G166530 vs. ExPASy Swiss-Prot
Match: Q4VBT5 (Probable E3 ubiquitin-protein ligase makorin-1 OS=Danio rerio OX=7955 GN=mkrn1 PE=2 SV=1)

HSP 1 Score: 241.9 bits (616), Expect = 1.2e-62
Identity = 145/421 (34.44%), Postives = 225/421 (53.44%), Query Frame = 0

Query: 2   SKRVLCKYFAHGACLKGEYCEFSHDWTA-PPSNVCTYYQKGRCAFGSRCRYDHVKVSHAE 61
           +K V C+YF HG C +GE C +SHD ++   + +C ++QKG CAFG RCRY+H K S  +
Sbjct: 19  TKHVTCRYFMHGLCKEGENCRYSHDLSSCKQTMICKFFQKGCCAFGDRCRYEHTKPSKQD 78

Query: 62  SSSNSQQSHVSESVSIAHPQLSRPLSPFPGSTSELAGSEIPSLPLRIESAWGGESEHPES 121
              +S+ S    +  +A      P+S  PG T+     E P     ++  W   +E    
Sbjct: 79  EVPSSKPSMPLTAAPLA--GTPEPVSDGPGGTT--GAQEKPQGSGAVD--WVNAAEFVPG 138

Query: 122 LDDWGNGEARVV-GPADI----------------PICSFAAAGSECPRGENCPHIHGDLC 181
               G  +  +  GP  +                 +C +AA G EC  G NC ++HGD+C
Sbjct: 139 QPYCGRADPVLCEGPGPLIEEEYEKEQANKEMKKQLCPYAAVG-ECRYGLNCAYLHGDVC 198

Query: 182 PTCGKQCLHPFRTDEREEHMKAC-EKKQKHLE---SLKNSQDIECSVCLERVLSKTEIAE 241
             CG Q LHP  T +R +H++AC E  +K +E   +++ S+D+ C VC+E V  KT  +E
Sbjct: 199 DMCGLQVLHPSDTSQRSQHIRACIEAHEKDMEISFAIQRSKDMMCGVCMEVVFEKTNPSE 258

Query: 242 RKFGLLSECDHPFCISCIRNWRNSSSTSGMDVNSALRACPICRKLSYFVIPSVIWYSSKE 301
           R+FG+LS C H +C+ CIR WR++        +  +++CP CR  S FVIPS  W   KE
Sbjct: 259 RRFGILSNCCHCYCLKCIRKWRSAKQFE----SKIIKSCPECRITSNFVIPSEYWVEDKE 318

Query: 302 EKQEIVDSYKSRLRSIDCKHFNFGNGNCPFGSSCFYKHLVIPGSYTWKYHRPPPRRPQSW 361
           EKQ+++  YK  + +  C++F+ G G CPFG++CFYKH    G    +   P P+R Q+ 
Sbjct: 319 EKQQLIQKYKDGMGTKPCRYFDEGRGTCPFGANCFYKHAFPDG----RLEEPQPQRRQNG 378

Query: 362 RTPPSRSNIVDMDAFFDMFEDLEDLD-FSDEDLDIEDLTLLEMALMQL--GCEANLSNDE 398
                R+        +D+ ++ E+ D F +ED ++    L EM LM L  G + ++++ E
Sbjct: 379 SN--GRNRNTRRTHLWDLLDERENSDSFDNEDEEMVRFELSEMLLMLLAAGTDDDVTDSE 422

BLAST of Cla97C09G166530 vs. ExPASy TrEMBL
Match: A0A0A0KBH2 (RING-type E3 ubiquitin transferase OS=Cucumis sativus OX=3659 GN=Csa_7G428290 PE=4 SV=1)

HSP 1 Score: 781.2 bits (2016), Expect = 2.1e-222
Identity = 369/398 (92.71%), Postives = 380/398 (95.48%), Query Frame = 0

Query: 1   MSKRVLCKYFAHGACLKGEYCEFSHDWTAPPSNVCTYYQKGRCAFGSRCRYDHVKVSHAE 60
           MSKRVLCKYFAHGACLKGEYCEFSHDWTAPPSNVCTYYQKGRCAFGSRCRYDHVKVSHAE
Sbjct: 1   MSKRVLCKYFAHGACLKGEYCEFSHDWTAPPSNVCTYYQKGRCAFGSRCRYDHVKVSHAE 60

Query: 61  SSSNSQQSHVSESVSIAHPQLSRPLSPFPGSTSELAGSEIPSLPLRIESAWGGESEHPES 120
           S SN QQSH SESVS+AH QLSRPLSPF GSTSELA SEIPSLPLR E+AW GE   P+ 
Sbjct: 61  SCSNVQQSHGSESVSVAHSQLSRPLSPFSGSTSELASSEIPSLPLREEAAWSGEFVRPDG 120

Query: 121 LDDWGNGEARVVGPADIPICSFAAAGSECPRGENCPHIHGDLCPTCGKQCLHPFRTDERE 180
           LDDW NGEARVVGPADIPICSFAAAG +CPRGENCPHIHGDLCPTCGKQCLHPFRTDERE
Sbjct: 121 LDDWVNGEARVVGPADIPICSFAAAGGDCPRGENCPHIHGDLCPTCGKQCLHPFRTDERE 180

Query: 181 EHMKACEKKQKHLESLKNSQDIECSVCLERVLSKTEIAERKFGLLSECDHPFCISCIRNW 240
           EH+KACEKKQKHLESLK+SQDIECSVCLERVLSKTE AERKFG+LSECDHPFCISCIRNW
Sbjct: 181 EHLKACEKKQKHLESLKHSQDIECSVCLERVLSKTETAERKFGILSECDHPFCISCIRNW 240

Query: 241 RNSSSTSGMDVNSALRACPICRKLSYFVIPSVIWYSSKEEKQEIVDSYKSRLRSIDCKHF 300
           RNSSSTSGMD+NSALRACPICRKLSYFVIPSVIWYSSKEEKQEIVDSYKSRLRSIDCKHF
Sbjct: 241 RNSSSTSGMDINSALRACPICRKLSYFVIPSVIWYSSKEEKQEIVDSYKSRLRSIDCKHF 300

Query: 301 NFGNGNCPFGSSCFYKHLVIPGSYTWKYHRPPPRRPQSWRTPPSRSNIVDMDAFFDMFED 360
           NFGNGNCPFGSSCFYKHLVIPGSYTWKYHRPPPR+PQSWRTPPSRSNIVDMDAFF+MFED
Sbjct: 301 NFGNGNCPFGSSCFYKHLVIPGSYTWKYHRPPPRQPQSWRTPPSRSNIVDMDAFFEMFED 360

Query: 361 LEDLDFSDEDLDIEDLTLLEMALMQLGCEANLSNDEES 399
           LEDLDFSDEDLD E LTLLEMALMQLGCEAN S+DEES
Sbjct: 361 LEDLDFSDEDLDFEYLTLLEMALMQLGCEANSSSDEES 398

BLAST of Cla97C09G166530 vs. ExPASy TrEMBL
Match: A0A5A7UYP2 (RING-type E3 ubiquitin transferase OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold459G00940 PE=4 SV=1)

HSP 1 Score: 755.4 bits (1949), Expect = 1.2e-214
Identity = 368/450 (81.78%), Postives = 379/450 (84.22%), Query Frame = 0

Query: 1   MSKRVLCKYFAHGACLKGEYCEFSHDWTAPPSNVCTYYQKGRCAFGSRCRYDHVKVSHAE 60
           MSKRVLCKYFAHGACLKGEYCEFSHDWTAPPSNVCTYYQKGRCAFGSRCRYDHVKVSH E
Sbjct: 1   MSKRVLCKYFAHGACLKGEYCEFSHDWTAPPSNVCTYYQKGRCAFGSRCRYDHVKVSHTE 60

Query: 61  SSSNSQQSHVSESVSIAHPQLSRPLSPFPGSTSELAGSEIPSLPLRIESAWGGESEHPES 120
           S SN QQSH SES+S+AH QLSRPLSPF GSTSELA SE+PSLPLR E+AW GE   P+ 
Sbjct: 61  SCSNVQQSHGSESLSVAHSQLSRPLSPFSGSTSELASSEMPSLPLRHETAWSGEFVRPDG 120

Query: 121 LDDWGNGEARVVGPADIPICSFAAAGSECPRGENCPHIHGDLCPTCGKQCLHPFRTDERE 180
           LDDWGNGEARVVGPADIPICSFAAAG +CPRGENCP IHGDLCPTCGKQCLHPFRTDERE
Sbjct: 121 LDDWGNGEARVVGPADIPICSFAAAGGDCPRGENCPRIHGDLCPTCGKQCLHPFRTDERE 180

Query: 181 EHMKACEKKQKHLESLKNSQDIECSVCLERVLSKTEIAERKFGLLSECDHPFCISCIRNW 240
           EH+KACEKKQKHLESLK+SQDIECSVCLERVLSKTE AERKFG+LSECDHPFCISCIRNW
Sbjct: 181 EHLKACEKKQKHLESLKHSQDIECSVCLERVLSKTETAERKFGILSECDHPFCISCIRNW 240

Query: 241 RNSSSTSGMDVNSALRACPICRKLSYFVIPSVIWYSSKEEKQEIVDSYKSRLRSIDCKHF 300
           RNSSSTSGMDVNSALRACPICRKLSYFVIPSVIWYSSKEEKQEIVDSYKSRLRSIDCKHF
Sbjct: 241 RNSSSTSGMDVNSALRACPICRKLSYFVIPSVIWYSSKEEKQEIVDSYKSRLRSIDCKHF 300

Query: 301 NFGNGNCPFGSSCFYK-------------------------------------------- 360
           NFGNGNCPFGSSCFYK                                            
Sbjct: 301 NFGNGNCPFGSSCFYKLLLSPLFDNFYGSIQVQSDVLTTLADLFAFCLSFNFIRADWLSN 360

Query: 361 --------HLVIPGSYTWKYHRPPPRRPQSWRTPPSRSNIVDMDAFFDMFEDLEDLDFSD 399
                   HLVIPGSYTWKYHRPPPRRPQSWRTPPSRSNIVDMDAFF+MFEDLEDLDFSD
Sbjct: 361 DTMFVVFQHLVIPGSYTWKYHRPPPRRPQSWRTPPSRSNIVDMDAFFEMFEDLEDLDFSD 420

BLAST of Cla97C09G166530 vs. ExPASy TrEMBL
Match: A0A6J1H7L6 (RING-type E3 ubiquitin transferase OS=Cucurbita moschata OX=3662 GN=LOC111460818 PE=4 SV=1)

HSP 1 Score: 743.4 bits (1918), Expect = 4.8e-211
Identity = 357/402 (88.81%), Postives = 371/402 (92.29%), Query Frame = 0

Query: 1   MSKRVLCKYFAHGACLKGEYCEFSHDWTAPPSNVCTYYQKGRCAFGSRCRYDHVKVSHAE 60
           MSKRVLCKYFAHGACLKGEYCEFSHDWTAPPSNVCTYYQKGRCAFGSRCRYDHVKVSHAE
Sbjct: 1   MSKRVLCKYFAHGACLKGEYCEFSHDWTAPPSNVCTYYQKGRCAFGSRCRYDHVKVSHAE 60

Query: 61  S--SSNSQQSHVSESVSIAHPQLSRPLSPFPGSTSELAGSEIPSLPLRIESAWGGESEHP 120
           S  SSNSQQ HVSESVSIAH  LS PLSP PGST ELAGS I  LP  +ESAW  E E P
Sbjct: 61  SSASSNSQQPHVSESVSIAHTLLSSPLSPSPGSTIELAGSRISCLPSHVESAWSVECELP 120

Query: 121 ESLDDWGNGEARVVGPADIPICSFAAAGSECPRGENCPHIHGDLCPTCGKQCLHPFRTDE 180
             LDDWG+ E R++ PAD+PICSF+AAG+ CPRG+NCPHIHGDLCPTCGKQCLHPFRTDE
Sbjct: 121 SGLDDWGSRETRIISPADLPICSFSAAGTNCPRGDNCPHIHGDLCPTCGKQCLHPFRTDE 180

Query: 181 REEHMKACEKKQKHLESLKNSQDIECSVCLERVLSKTEIAERKFGLLSECDHPFCISCIR 240
           REEHMKACEKKQKHLESLK+SQ+IECSVCLERVLSKTEIAERKFGLLSECDHPFCISCIR
Sbjct: 181 REEHMKACEKKQKHLESLKHSQEIECSVCLERVLSKTEIAERKFGLLSECDHPFCISCIR 240

Query: 241 NWRNSSSTSGMDVNSALRACPICRKLSYFVIPSVIWYSSKEEKQEIVDSYKSRLRSIDCK 300
           NWRNSSSTSGMDVNSALRACPICRKLSYFVIPSVIWYSSKEEKQEIVDSYKS+LRSIDCK
Sbjct: 241 NWRNSSSTSGMDVNSALRACPICRKLSYFVIPSVIWYSSKEEKQEIVDSYKSKLRSIDCK 300

Query: 301 HFNFGNGNCPFGSSCFYKHLVIPGSYTWKYHRPPPRRPQSWRTPPSRSNIVDMDAFFDMF 360
           HFNFGNGNCPFGSSCFYKHLV PGSYTWKYHRPPP RP+S RTPPSRSNIVDMDAFFDMF
Sbjct: 301 HFNFGNGNCPFGSSCFYKHLVKPGSYTWKYHRPPPPRPRSQRTPPSRSNIVDMDAFFDMF 360

Query: 361 EDLEDLDFSDEDLDIEDLTLLEMA--LMQLGCEANLSNDEES 399
           EDLEDLDFS EDLD EDLT LEMA  LMQLGCEANLS+DEE+
Sbjct: 361 EDLEDLDFSAEDLDFEDLTPLEMAFVLMQLGCEANLSSDEEN 402

BLAST of Cla97C09G166530 vs. ExPASy TrEMBL
Match: A0A6J1KQR4 (RING-type E3 ubiquitin transferase OS=Cucurbita maxima OX=3661 GN=LOC111497821 PE=4 SV=1)

HSP 1 Score: 739.2 bits (1907), Expect = 9.1e-210
Identity = 353/402 (87.81%), Postives = 371/402 (92.29%), Query Frame = 0

Query: 1   MSKRVLCKYFAHGACLKGEYCEFSHDWTAPPSNVCTYYQKGRCAFGSRCRYDHVKVSHAE 60
           MSKRVLCKYFAHGACLKGEYCEFSHDWTAPPSNVCTYYQKGRCAFGSRCRYDHVK SHAE
Sbjct: 1   MSKRVLCKYFAHGACLKGEYCEFSHDWTAPPSNVCTYYQKGRCAFGSRCRYDHVKASHAE 60

Query: 61  S--SSNSQQSHVSESVSIAHPQLSRPLSPFPGSTSELAGSEIPSLPLRIESAWGGESEHP 120
           S  SSNSQQ H+SESVSIAH  LS PLSP PGST ELAGS+I  LP  +ESAW  E E P
Sbjct: 61  SSASSNSQQPHMSESVSIAHTLLSSPLSPSPGSTIELAGSKISCLPSHVESAWSVECELP 120

Query: 121 ESLDDWGNGEARVVGPADIPICSFAAAGSECPRGENCPHIHGDLCPTCGKQCLHPFRTDE 180
             LDDWG+ E R++ PAD+PICSF+AAG+ CPRG+NCPHIHGDLCPTCGKQCLHPFRTDE
Sbjct: 121 SGLDDWGSRETRIISPADLPICSFSAAGTNCPRGDNCPHIHGDLCPTCGKQCLHPFRTDE 180

Query: 181 REEHMKACEKKQKHLESLKNSQDIECSVCLERVLSKTEIAERKFGLLSECDHPFCISCIR 240
           REEHMKACEKKQKHLESLK+SQ+IECSVCLERVLSKTEIAERKFGLLSECDHPFCISCIR
Sbjct: 181 REEHMKACEKKQKHLESLKHSQEIECSVCLERVLSKTEIAERKFGLLSECDHPFCISCIR 240

Query: 241 NWRNSSSTSGMDVNSALRACPICRKLSYFVIPSVIWYSSKEEKQEIVDSYKSRLRSIDCK 300
           NWRNSSSTSGMDVNSALRACPICRKLSYFVIPSVIWYSSKEEKQEIVDSYKS+LRSIDCK
Sbjct: 241 NWRNSSSTSGMDVNSALRACPICRKLSYFVIPSVIWYSSKEEKQEIVDSYKSKLRSIDCK 300

Query: 301 HFNFGNGNCPFGSSCFYKHLVIPGSYTWKYHRPPPRRPQSWRTPPSRSNIVDMDAFFDMF 360
           HFNFGNGNCPFGSSCFYKHLV PGSYTWKYHRPPP RP+S RTPPSRSNIVDMDAFFDMF
Sbjct: 301 HFNFGNGNCPFGSSCFYKHLVKPGSYTWKYHRPPPHRPRSQRTPPSRSNIVDMDAFFDMF 360

Query: 361 EDLEDLDFSDEDLDIEDLTLLEMA--LMQLGCEANLSNDEES 399
           +DLEDLDFS EDLD E+LT LEMA  LMQLGCEANLS+DEE+
Sbjct: 361 QDLEDLDFSAEDLDFEELTPLEMAFVLMQLGCEANLSSDEEN 402

BLAST of Cla97C09G166530 vs. ExPASy TrEMBL
Match: A0A6J1GQ00 (E3 ubiquitin-protein ligase makorin-like isoform X1 OS=Cucurbita moschata OX=3662 GN=LOC111456442 PE=4 SV=1)

HSP 1 Score: 738.0 bits (1904), Expect = 2.0e-209
Identity = 356/402 (88.56%), Postives = 372/402 (92.54%), Query Frame = 0

Query: 1   MSKRVLCKYFAHGACLKGEYCEFSHDWTAPPSNVCTYYQKGRCAFGSRCRYDHVKVSHAE 60
           MSKRVLCKYF+HGACLKGEYCEFSHDW+APPSNVCTYYQKGRCAFGSRCRYDHVKVS AE
Sbjct: 1   MSKRVLCKYFSHGACLKGEYCEFSHDWSAPPSNVCTYYQKGRCAFGSRCRYDHVKVSQAE 60

Query: 61  S--SSNSQQSHVSESVSIAHPQLSRPLSPFPGSTSELAGSEIPSLPLRIESAWGGESEHP 120
           S  SSNSQQ HVSESVSIAHP LSRPLSP PGSTSELAGS+I   P  ++  W GESEHP
Sbjct: 61  SSASSNSQQPHVSESVSIAHPLLSRPLSPLPGSTSELAGSKISCPPSHVQIVWSGESEHP 120

Query: 121 ESLDDWGNGEARVVGPADIPICSFAAAGSECPRGENCPHIHGDLCPTCGKQCLHPFRTDE 180
           + LDDW NGE R+VGPADIPICSFAAAGS+CPRG+NCPHIHGDLCPTCGKQCLHP RTDE
Sbjct: 121 DGLDDWRNGETRIVGPADIPICSFAAAGSDCPRGQNCPHIHGDLCPTCGKQCLHPSRTDE 180

Query: 181 REEHMKACEKKQKHLESLKNSQDIECSVCLERVLSKTEIAERKFGLLSECDHPFCISCIR 240
           REEH+KACEKKQKHLESLK SQDIECSVCLERVLSKTEIAERKFGLLSECDHPFCISCIR
Sbjct: 181 REEHIKACEKKQKHLESLKRSQDIECSVCLERVLSKTEIAERKFGLLSECDHPFCISCIR 240

Query: 241 NWRNSSSTSGMDVNSALRACPICRKLSYFVIPSVIWYSSKEEKQEIVDSYKSRLRSIDCK 300
           NWRN+SSTSGMDVNSALRACPICRKLSYFVIPSVIWYSSKEEKQEIVDSYKSRLRSIDCK
Sbjct: 241 NWRNTSSTSGMDVNSALRACPICRKLSYFVIPSVIWYSSKEEKQEIVDSYKSRLRSIDCK 300

Query: 301 HFNFGNGNCPFGSSCFYKHLVIPGSYTWKYHRPPPRRPQSWRTPPSRSNIVDMDAFFDMF 360
           HFNFGNGNCPFGSSCFYKHLV PGSY+WKYHRPPPRRP+S RT P+R NIVDMDAFFDMF
Sbjct: 301 HFNFGNGNCPFGSSCFYKHLVKPGSYSWKYHRPPPRRPRSQRT-PNRYNIVDMDAFFDMF 360

Query: 361 EDLEDLDFSDEDLDIEDLTLLEMA--LMQLGCEANLSNDEES 399
           EDLEDLDFS EDLD EDLT LEMA  LMQLG EAN S+DEE+
Sbjct: 361 EDLEDLDFSAEDLDFEDLTPLEMAFVLMQLGREANSSSDEEN 401

BLAST of Cla97C09G166530 vs. TAIR 10
Match: AT3G08505.2 (zinc finger (CCCH-type/C3HC4-type RING finger) family protein )

HSP 1 Score: 410.2 bits (1053), Expect = 1.9e-114
Identity = 197/322 (61.18%), Postives = 231/322 (71.74%), Query Frame = 0

Query: 1   MSKRVLCKYFAHGACLKGEYCEFSHDWTAPPSNVCTYYQKGRCAFGSRCRYDHVKVSHAE 60
           MS R+LCK+F HG+CLKGE CEFSHD   PP+NVCT+YQK  C +GSRCRYDHV+     
Sbjct: 1   MSDRILCKFFVHGSCLKGENCEFSHDSKDPPNNVCTFYQKRICLYGSRCRYDHVR----- 60

Query: 61  SSSNSQQSHVSESVSIAHPQLSRPLSPFPGSTSELAGSEIPSLPLRIESAWGGESEHPES 120
           ++SN   S  SES       L R +S  P    +  G                     ++
Sbjct: 61  AASNLPLSSDSES-------LDRSISTTPSRHLQQQG---------------------DN 120

Query: 121 LDDWGNGEARVVGPADIPICSFAAAGSECPRGENCPHIHGDLCPTCGKQCLHPFRTDERE 180
            D   +     + P + PICSFAAAG +CPRG  CPH+HGDLC TCGK+CLHPFR +ERE
Sbjct: 121 NDGDKSSNVYCIHPREYPICSFAAAG-DCPRGNQCPHMHGDLCNTCGKKCLHPFRPEERE 180

Query: 181 EHMKACEKKQKHLESLKNSQDIECSVCLERVLSKTEIAERKFGLLSECDHPFCISCIRNW 240
           EH K CEKKQKH+E+LK SQDIECSVCL+R+LSK    ERKFGLL+ECDHPFCI CIRNW
Sbjct: 181 EHTKECEKKQKHIEALKQSQDIECSVCLDRILSKATPGERKFGLLTECDHPFCIQCIRNW 240

Query: 241 RNSSSTSGMDVNSALRACPICRKLSYFVIPSVIWYSSKEEKQEIVDSYKSRLRSIDCKHF 300
           R+S+  SGMDVNS LRACPICRKLSYFV+PSV+WYSS EEK+EI+D YK++LRSIDCKHF
Sbjct: 241 RSSAPVSGMDVNSTLRACPICRKLSYFVVPSVVWYSSPEEKKEIIDIYKAKLRSIDCKHF 288

Query: 301 NFGNGNCPFGSSCFYKHLVIPG 323
           NFGNGNCPFG+SCFYKH    G
Sbjct: 301 NFGNGNCPFGASCFYKHAYSDG 288

BLAST of Cla97C09G166530 vs. TAIR 10
Match: AT3G08505.1 (zinc finger (CCCH-type/C3HC4-type RING finger) family protein )

HSP 1 Score: 410.2 bits (1053), Expect = 1.9e-114
Identity = 197/322 (61.18%), Postives = 231/322 (71.74%), Query Frame = 0

Query: 1   MSKRVLCKYFAHGACLKGEYCEFSHDWTAPPSNVCTYYQKGRCAFGSRCRYDHVKVSHAE 60
           MS R+LCK+F HG+CLKGE CEFSHD   PP+NVCT+YQK  C +GSRCRYDHV+     
Sbjct: 1   MSDRILCKFFVHGSCLKGENCEFSHDSKDPPNNVCTFYQKRICLYGSRCRYDHVR----- 60

Query: 61  SSSNSQQSHVSESVSIAHPQLSRPLSPFPGSTSELAGSEIPSLPLRIESAWGGESEHPES 120
           ++SN   S  SES       L R +S  P    +  G                     ++
Sbjct: 61  AASNLPLSSDSES-------LDRSISTTPSRHLQQQG---------------------DN 120

Query: 121 LDDWGNGEARVVGPADIPICSFAAAGSECPRGENCPHIHGDLCPTCGKQCLHPFRTDERE 180
            D   +     + P + PICSFAAAG +CPRG  CPH+HGDLC TCGK+CLHPFR +ERE
Sbjct: 121 NDGDKSSNVYCIHPREYPICSFAAAG-DCPRGNQCPHMHGDLCNTCGKKCLHPFRPEERE 180

Query: 181 EHMKACEKKQKHLESLKNSQDIECSVCLERVLSKTEIAERKFGLLSECDHPFCISCIRNW 240
           EH K CEKKQKH+E+LK SQDIECSVCL+R+LSK    ERKFGLL+ECDHPFCI CIRNW
Sbjct: 181 EHTKECEKKQKHIEALKQSQDIECSVCLDRILSKATPGERKFGLLTECDHPFCIQCIRNW 240

Query: 241 RNSSSTSGMDVNSALRACPICRKLSYFVIPSVIWYSSKEEKQEIVDSYKSRLRSIDCKHF 300
           R+S+  SGMDVNS LRACPICRKLSYFV+PSV+WYSS EEK+EI+D YK++LRSIDCKHF
Sbjct: 241 RSSAPVSGMDVNSTLRACPICRKLSYFVVPSVVWYSSPEEKKEIIDIYKAKLRSIDCKHF 288

Query: 301 NFGNGNCPFGSSCFYKHLVIPG 323
           NFGNGNCPFG+SCFYKH    G
Sbjct: 301 NFGNGNCPFGASCFYKHAYSDG 288

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
XP_038898014.12.8e-22995.23E3 ubiquitin-protein ligase makorin isoform X1 [Benincasa hispida] >XP_038898015... [more]
XP_038898016.15.1e-22395.34E3 ubiquitin-protein ligase makorin isoform X2 [Benincasa hispida][more]
XP_031744426.14.3e-22292.71E3 ubiquitin-protein ligase makorin isoform X1 [Cucumis sativus] >XP_031744427.1... [more]
KAA0058639.12.5e-21481.78E3 ubiquitin-protein ligase makorin [Cucumis melo var. makuwa] >TYK10446.1 E3 ub... [more]
XP_023547951.12.6e-21189.05E3 ubiquitin-protein ligase makorin-like isoform X1 [Cucurbita pepo subsp. pepo][more]
Match NameE-valueIdentityDescription
E0X9N42.5e-11963.72Putative RING-type E3 ubiquitin transferase C3H69 OS=Arabidopsis thaliana OX=370... [more]
Q5ZA072.5e-11661.90E3 ubiquitin-protein ligase makorin OS=Oryza sativa subsp. japonica OX=39947 GN=... [more]
Q6IDS62.6e-11361.18E3 ubiquitin-protein ligase makorin OS=Arabidopsis thaliana OX=3702 GN=MKRN PE=2... [more]
Q8JFF33.3e-6336.83Probable E3 ubiquitin-protein ligase makorin-1 OS=Seriola quinqueradiata OX=8161... [more]
Q4VBT51.2e-6234.44Probable E3 ubiquitin-protein ligase makorin-1 OS=Danio rerio OX=7955 GN=mkrn1 P... [more]
Match NameE-valueIdentityDescription
A0A0A0KBH22.1e-22292.71RING-type E3 ubiquitin transferase OS=Cucumis sativus OX=3659 GN=Csa_7G428290 PE... [more]
A0A5A7UYP21.2e-21481.78RING-type E3 ubiquitin transferase OS=Cucumis melo var. makuwa OX=1194695 GN=E56... [more]
A0A6J1H7L64.8e-21188.81RING-type E3 ubiquitin transferase OS=Cucurbita moschata OX=3662 GN=LOC111460818... [more]
A0A6J1KQR49.1e-21087.81RING-type E3 ubiquitin transferase OS=Cucurbita maxima OX=3661 GN=LOC111497821 P... [more]
A0A6J1GQ002.0e-20988.56E3 ubiquitin-protein ligase makorin-like isoform X1 OS=Cucurbita moschata OX=366... [more]
Match NameE-valueIdentityDescription
AT3G08505.21.9e-11461.18zinc finger (CCCH-type/C3HC4-type RING finger) family protein [more]
AT3G08505.11.9e-11461.18zinc finger (CCCH-type/C3HC4-type RING finger) family protein [more]
InterPro
Analysis Name: InterPro Annotations of Watermelon (97103) v2.5
Date Performed: 2022-01-31
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
NoneNo IPR availableCOILSCoilCoilcoord: 179..199
NoneNo IPR availableCOILSCoilCoilcoord: 392..398
NoneNo IPR availablePFAMPF14608zf-CCCH_2coord: 7..25
e-value: 0.12
score: 13.0
coord: 296..317
e-value: 0.0096
score: 16.5
NoneNo IPR availableGENE3D1.20.120.1350coord: 1..32
e-value: 1.5E-6
score: 29.7
NoneNo IPR availableGENE3D3.30.1370.210coord: 33..78
e-value: 8.3E-6
score: 27.8
NoneNo IPR availablePANTHERPTHR11224:SF52ZINC FINGER CCCH DOMAIN-CONTAINING PROTEIN 69-LIKEcoord: 1..319
NoneNo IPR availableCDDcd16521RING-HC_MKRNcoord: 204..262
e-value: 1.83201E-17
score: 73.8902
NoneNo IPR availableSUPERFAMILY57850RING/U-boxcoord: 192..268
IPR000571Zinc finger, CCCH-typeSMARTSM00356c3hfinal6coord: 29..55
e-value: 0.0013
score: 28.0
coord: 135..161
e-value: 0.14
score: 21.3
coord: 291..319
e-value: 0.36
score: 19.8
coord: 1..27
e-value: 5.7E-5
score: 32.5
IPR000571Zinc finger, CCCH-typePROSITEPS50103ZF_C3H1coord: 1..28
score: 13.685178
IPR000571Zinc finger, CCCH-typePROSITEPS50103ZF_C3H1coord: 134..162
score: 12.192311
IPR000571Zinc finger, CCCH-typePROSITEPS50103ZF_C3H1coord: 291..320
score: 13.290835
IPR000571Zinc finger, CCCH-typePROSITEPS50103ZF_C3H1coord: 29..56
score: 15.112321
IPR001841Zinc finger, RING-typeSMARTSM00184ring_2coord: 204..261
e-value: 1.7E-7
score: 40.9
IPR001841Zinc finger, RING-typePROSITEPS50089ZF_RING_2coord: 204..262
score: 12.371254
IPR018957Zinc finger, C3HC4 RING-typePFAMPF00097zf-C3HC4coord: 204..261
e-value: 1.5E-7
score: 31.2
IPR013083Zinc finger, RING/FYVE/PHD-typeGENE3D3.30.40.10Zinc/RING finger domain, C3HC4 (zinc finger)coord: 173..267
e-value: 4.2E-10
score: 40.9
IPR041367E3 ligase, CCCH-type zinc fingerPFAMPF18044zf-CCCH_4coord: 34..53
e-value: 2.9E-7
score: 30.1
IPR045072MKRN-likePANTHERPTHR11224MAKORIN-RELATEDcoord: 1..319
IPR017907Zinc finger, RING-type, conserved sitePROSITEPS00518ZF_RING_1coord: 228..237
IPR036855Zinc finger, CCCH-type superfamilySUPERFAMILY90229CCCH zinc fingercoord: 32..53
IPR036855Zinc finger, CCCH-type superfamilySUPERFAMILY90229CCCH zinc fingercoord: 3..26

Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
Cla97C09G166530.2Cla97C09G166530.2mRNA


GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
molecular_function GO:0003677 DNA binding
molecular_function GO:0046872 metal ion binding