Cla97C08G158680 (gene) Watermelon (97103) v2.5

Overview


Name	Cla97C08G158680
Type	gene
Organism	Citrullus lanatus subsp. vulgaris cv. 97103 (Watermelon (97103) v2.5)
Description	tRNA 4-demethylwyosine synthase (AdoMet-dependent)
Location	Cla97Chr08: 25987898 .. 25995070 (+)
RNA-Seq Expression	Cla97C08G158680
Synteny	Cla97C08G158680

Sequences

The following sequences are available for this feature:

Gene sequence (with intron)

Legend: exonfive_prime_UTRCDSpolypeptidethree_prime_UTR

Hold the cursor over a type above to highlight its positions in the sequence below.

ACAAGAATGAAGCGAAGGATGTTCGCTTTCAGTTTACGCCCAATCAATTAACCTAACAGCAGCTTAAACCCAATCAACTCGCCATGTCCTTTTCTTCACGCTCCGCGAAGCTCACCCTTCTAGCTCTTCTCTCTGCCTCCACTTTGTATTGTTTCTACAAGTCCCGCCGTCTCAGAAAGCTTAAACTCTCCTTAAACCCTACTCCTTGTTCTCGCAGACCCAAACTGTTTTTTATTTCTCAAACTGGTACTTCCAAAGCCCTAGCTCATCGTCTGCTTAACGTTTTGAATTCCAATGGGTTGGTCTTTGATCTTGTCGATCCCAAGGATTATGAACCTGAAGATCTGTTTAAGGAGATTCTAGTTGTGTTCGTTGCTTCCACTTGGGAAGATGGTGGACCGCCTCCTCATGCTAAATTTCTCGCTAATTGGCTTTCTGAAAGCGCGGAGGATTTTAGAGTTGGTTCGTTGTTGCTTTCTCAGTGTAAATTTGCTGTGTTTGGCGTTGGTAGTCGAGCTTATGGAGAGACATTCAATGCGGTTGCAATGGACTTTTCCAAGCGGATGAAGGCATTGGGTGCGAAGGAGATTTTAGCGGTTGGTGAAGGAGATATTGATGGAGGTGATATAGATAAGTATTTTGATGATTGGAGTCGGCAATTGCTGAAGGTTGTGAAGGTTGATGCTGTGGGAACTGGTGTAGATTTAGATTCTGGGATTGCTGGGGACAGTGATGCTGAAAGTGTTGAGGAGGATGAAGAAGAAGATACCGACCTCGGGGAGGAGGACATTGTTGATCTTGAGGATATTGCTGGTAAGGGTCCTTCGAGAAAGTCTACGAATGTAGTCGAAACTAATGGGAAATTGAATGGGAAAAAGGTTATGGTGACCCCGGTGATTAGAGCGAGCTTAGAAAAGCAGGTAAATTCGCTAGCTGTATTTTCCAAATTGTCTGAATAACTGCTTGTTTTTAAGGCAATTTCTGAGTGTCTTGTGAATCGATACAAATTTGGACCGACTTGATAATTTGTCTTTCCAAGGGGCTAGTTGGGTTGAATTAGAAGGCTTACTTCTTTTTCTTGTGTTGTTTGGTTGGCTCTTTGAATGATTTTTCTTATGCAGGGATATAAAATTATTGGCTCACATAGCGGAGTGAAAATATGTAGATGGACGAAGTCTCAGCTTCGAGGGCGAGGAGGTTGCTATAAACATTCATTTTATGGCATAGAAAGTCATCGGTAATGTCCCACTTCACATGCTATTTTGCTTGGCCATAGCGTGTCTTATTTCCTTTCATGGCCATTGTAATTCGTGATGTTTTAATGCATTAAGTGGCAATGGCATAGAGCTTCCCCTTCCATAGATAAAATTTATGCCTCTTTACTTTGGTAAATAAATAATTTCACTTCTTGAGGAGGATGGAGATGGATGATAGAATATGACTGAGAGTAGCCTTCAAATCACTGTACAAGTCACAATATCCGATGGAAATAATTTTGTTTGATAGTCTTTGCTCGCCCAAATTTTACATCCAGCTGCTTCTAATTTCATTACGGGAATCATTATTGTTTTGCCTGCATGGCTTATATCTTAAATCCTCTTTTTGTTGTCTTGCTGGTGCAAGACGTGTGTTCAGAGGTCCTTTTGTTTGACTTGCATTATGGGTTCTTCTTCATCCTTGTTAAGTTTACTTCTCTGACCTCTAGGGAATTTCAAGTTGACTCTTCAATTTCCTTTCACTCAACAAGGCTTTTCCTAAGCTCTTAACTTTTTTAATAGACTGGGAAAAACTATCTATGGGTTAGTGCTGTTGCCCACAGTAACTTTTCAAATTTGAGAACAGATGCATGGAGGCAACTCCCAGTTTGGCTTGTGCCAACAAATGTGTTTTCTGCTGGAGGCATCACACAAATCCAGTAGGAAAGAGTTGGCAGTGGGAGATGGATGATCCATTGGATATTGTGAATTCTGCAATCGATTTACATACAAAAATGATCAAGCAAATGAAAGGTGTTCCAGGTTAGTGCTCCTTCCTTACAGCACTGGCTTGAATAGTAATTTTTTGAAGAGTGCATTTTCTTGGATATACTCAATTGTTCCCATGATTTGCTGATTGTAGGATCTGAATTTTTGGTAAATCATCAATATTGTTAATGTTAATTGTTCTTTTACAACTGTCATAGAAATGCATTGGCATAGCATGAGTTTTGTGGCTAAATAGTGATCATGAGTAGTAGAGACCGAAAGTAACCAGATTCCAGAATGTCTGGCTAGAATCTGAAGTTGCTGTATTTTATTTTATAGTAGCCATCAGTGAGAATCTCGAGAGGATGCCTGAAATGGAAGTTATTTTAAAGATTCTTCTTACTTGTAAAATATTATTATTATTATTATTATTATTATTATTTTTTTTTTTTGCAGTAATCAACGACATGTTGTGGATGAGTTTATGTTCACGTAATTTATTTATTTTATTTGGAAAGAAGTTAATGTCAACAAATTTGAAATGCAATTACCAACGTTTGCGTTGTGCAAAGCTTGCTCCTTTACAAAACAAGTGCAAAGCTAATTTTAGTTATTCAGATCCACTCATGTTAATAAAATAGAAGTTCATGTCCAATGCTAAAAAGTGAAAACTAGTTTTGCAAACCATGAATGCTTCAAGGTGTAAAAGCATATTTTCATTCATTGGTAGTTGGGATTTTAGTGATGAGGTGAGTCATTGATGCATGTCTTCCCGCAGGTGTTACGCAGGAGAAATTGGACGAAGGCCTGTCCCCAAGACATTGTGCCTTGTCTCTTGTTGGTGAACCTATTATGTACCCAGAGATTAATACACTTGTTGATGAATTGCATCGAAGGCGGATTTCAACTTTCTTGGTAACGAATGCTCAGTTTCCTGATAAAATAAAACTGCTGAAGCCAGTCACCCAGGTTTGCGTGCTTTACACATTTTCTTTTCTACATAACATCAAATATCAGGCTTGTACTCATGATACTAGATTACTTATCTGATTTCCACGATGCATGCAGCTGTATGTAAGTGTAGATGCTGCGACGAAGGAGAGCTTGAAGGCAATTGATAGACCACTTTTTGGAGACTTCTGGGAGCGATTCATTGTAGGTTTCCTCACACCCTCACCTCTTTCCGGGTCTGGGTCCTGAGCTAGGCTTCATCGGAATTAATGAGAAAAGTAAATCTTATGGGGGATGTTAAAATAAACAATTTAAAAGTAAACTGTTCCCAGCTCAAAACCTCATTTAGCCAATCACTTCTTGCCCACACTGGGTGCTCAATGGTCAAATACATCTACCTTACAAAGTAAAATTATTAAGCAACAAATGTGCTTCACGCTTCTAAAAGGGAGAAACCCTTTCTAAAAGTTCTTCCTATCTTGAAGGAAGCATTTCATGATGAGGAGCAGGTAGTCTTAGTAATTTTCAGTTTGACAATAAAAGTTTCATTAATTGGTGCAGGTTATTGCTTTTACCCTAAGTTTTCATAGAGAAGGCTCTGTCATCTAGTCTCCAGCCCCAATTGACTTATATTGTGTTTAATACCCATATTCTCCTATTAACTTCTTCCTATGATTATTTATCAGCTAGAATTATGGATTTCTAGAGAAGAGAATTTCAGTGCCTTTGGATAACTTGACTTGTGTATTTTATTTTTTCTTTTATATATATATATTAAAATCATTGACAGAAAACCAACTTTGTGAAAGAACAACAAACAAGGAAAGACTCACAAAAAGAAGGGATCAATCGAAAGAGGGGGATGGTAAGCAATACTCAAAAGTATTTACAAGGAAGAACATCAACCTTCCGGTAACCTAAAAAAATGAACAATGACGAATTACCAAATCAATTTGAATTAAGATCCCAAAGAGAACCTAAACCAAAACAAAAAATAATGACAAAATAAATTTGTATTAAGAGCCCATAGAGAAGCCTAAATGACCACTGGATTCCACACTTCTCTCCAAGAGCAATCTCTTTCTAGGAATACTCTTTTATTCCTTTCCAACTAGATAACCTACAAAACATTAGAGAGAGCCAGCTGGTCTGCCAAAGAACCTGACCTTGTTAGAAGTTGATTACGTTGTTAGTATTATTTTAAACGTCACATCATAGGCTGAGTGTTGATTCTCTTATGCAACTTATTAAATCATCGCTCTATCAACAAAGTTTTATCATAAGTCTGTTAGGTACTTAAGCACCTTGGAGAACCACACCCCAAAAACCAGCTCATAAGGTGAGAGAGTCGAACCACTTAAGTACCATATAGGTCATCCCGTTCTAACCGATATGAGACAAAGGTAACCCATACTACCTTAGTTTCCTAATAAAGTCATAACATTAAAAATCAATATTTAGCAAAGCTACAAAGGATATAATGCACATGACTGCATCACGTCATTATTGAATCTTTTTTGTTGACAGCTAAAAAGGTTCTTCAGCTTTATATCTTGTTGCTTTGTTGCCGTGATCTGCTTAGTAGCTTGCATAATGTTGCAGGATTCCTTGAAAGCTCTAAATGATAAGCAACAGCGAACAGTTATTAATGATTTATGTGCCTCCATAATGTTGCAGGATTCCTTGAAAGCTCTAAATGACAAGCAACAGCGAACAGTTTATCGATTGACGTTGGTAAAAGGGTGGAACACAGAAGATATTGATGCCTATTCGAATCTCTTTAGCATTGGTAGACCTGATTTTGTGGAAATCAAAGGCGTCACATATTGTGGATCGTGAGTTTATTTTTATTTATTTTGCATTAGGTGAGGAGGATATTGATTTGAAAATTGTGGGAGCTTAACGAGTCTAATGTTCTTCTGTAGGTCTAATACTTCAAAGCTGACAATGGAGAACGTTCCTTGGCATTCTGACGTCAAGTCTTTTTCCGAGGCTTTAGCTGCAAAAAGTCGTGGGGAGTATGAAGTTGCTTGTGAGCATGTGCACTCATGTTGCGTTCTTTTGGCTAAAACTGACAAATTTAAGGTCGATGGCCAGTGGTTCACTTGGATAGATTATGAGAAATTCCATGACCTGGTTTGTATAGATGACAACTAATTTACCTGAAACCTAAGATGTTTAAGCTCCTTCCATTAAATTTTATCCTTCTCGAAAATTTCAGGTGGCCTCTGGAAAACCTTTTGATAGCAAGGATTACATGGCTCCAACACCATCATGGGCTGTATATGGCTCTGACGAAGGTGGATTTGATCCTGATCAATCTCGGTACCGGAAGGAGCGACATCATAGACCAAAACCCACATCCTGATAGCTTTGAATGTCCGGGTCAGTTTTGTTTAATCAATTGGTTAATCAATTTTCTTTGAGGATTTCTGTAGTTTATATTGTTCTCCTGTGAAACAGCCTAGCTTGGCATTACTTATTTTTAAAAAGTGTCTTGTGTGGCATTTCCGCACATACACATGTTTAAACTCGTACTACTTTTCTAATTATAGAAAATATAGAGCTTGCTTGGTAATGAAATTTGGAACGTTAAAAGACACTTTGTTTTCTAAACTTTTGTGGAATAATATTTTAGTCGCTCTATTTTTAAATTCGTAATAATTTAGTTCATGAACTATAGTGTATAACAATTTAGTATGGTGACAAATTCATTCCTATTAAATTCCATCAAAATTAAGTGTCAATTTTTATAACATAACAAATTAATCTTTTTTTTTTTTTTTTGAAACACATTTAGTCCATAATAAGTTATCAATTTACCTATGTAAGAAATCTTATTGAATCTTAACAACATCCGACCAAGTTGTAATAAATTTTAAAATAAAAGGTCTAAATTGTTAAATAGTAAAGTTCATAGGCTAAATTGTTACACGATTGAAAGTATAAGGACTAAATCGTTACAAACTAAAGTTTATGGACTAAATTGTTATTTCCATGAACTAAAGAGTGTTGGTTTAACCAATTTATCATTAAACTTAATTTCTTTTGACTGTTACTGAGAATTCTTCTTGGTGAAGAAATAATGGAAAGTTATGTTTTCTTTTATCGTTTTTCTGGTTTTCTTTATTACGATGAATTCTATACAACTGTAGGTTTTGGTGTATTTATGCGTAGTTGTGATTTTTTTCATAAATGGAAATGTTTATTTGGCTCCGTCCTAACCATTCTCCTTAGACTGTAAAAGACTCCCATAAGAGGTACGTGAATCTGCCTCACACTAACTGCTAACGTTCAACATCTTTCCAGAGTCATGTCACCATCCACTTAAAGGTTGTCTACCTTTTTAGATTTAGCAGCCGACGAATGACATTATTTCAACCTTAGAAAGCCGGGTCGGGCACCATGGCTCTGTCCCATCAGTAGATATAGTCCGTGAGGTTCAAAAAGATCCTGGCATGTTGCCATCTACCTTCCATCATACTAAAATAATAATTGAACCTACAATATTTTTACTGGATCCAACATTAAGAATCAAAGTGTTAGTCCAATCAAATCAAGATCTACCTTACCTTCAATATTTACTTGATATAACCTTCAGAATGAAAGAATCAAGTCCAGAAAAATCAAGATGTGTAATAGTAGTATTGTAGTAAACTAGTAATATTAGCTTTCAAAGTTTTTCATGATATACAACAGGCCTTGTGGATGTTTTTCCTGCTATAAAAGTTGAAACAGCTACCTTTTATAGGATTATTCCGAAGCAAAGCTTGTCAATGCCAACTCCATTGGATCAGTTCGAATGCAACCGGTGGAGAAAAAAGTACTCTTATCGGTTGGGAGTGGCAAGGCAGTGAAGGTAGAGTCTTGAAAATGGTCACTCAGCATTACTTTCAGAACTCCAAATTTACTTCCAGAATCTCTAAGTCTGAATTATTGATGGATGTTGAAATCTTAAGATGTATCTTGTTGCATTAAAGAATGATTTGTGGTACAGAGTGTGCTGGAGGTGCTGGTAAATAACCCTGGGTAAAAGATGGAGAGACAAGATCCTCATCTGCTGGTCATTTGTCGCCAGGTAAATTTATTTGGTGATATGTGAAGAAATTGACCTTAGAACATTGTTTCTTCTATTATTTGGTAGTTTGTTTTAGGGAGGTCTACATACCATGAACCAAAGAGCAAGGATGACCATGGATGGATTTAGATGTATTTGAAAGGCTTAGGAATTCAGAATTAGTGTGTTGTTTTGTAGGAT

mRNA sequence

ACAAGAATGAAGCGAAGGATGTTCGCTTTCAGTTTACGCCCAATCAATTAACCTAACAGCAGCTTAAACCCAATCAACTCGCCATGTCCTTTTCTTCACGCTCCGCGAAGCTCACCCTTCTAGCTCTTCTCTCTGCCTCCACTTTGTATTGTTTCTACAAGTCCCGCCGTCTCAGAAAGCTTAAACTCTCCTTAAACCCTACTCCTTGTTCTCGCAGACCCAAACTGTTTTTTATTTCTCAAACTGGTACTTCCAAAGCCCTAGCTCATCGTCTGCTTAACGTTTTGAATTCCAATGGGTTGGTCTTTGATCTTGTCGATCCCAAGGATTATGAACCTGAAGATCTGTTTAAGGAGATTCTAGTTGTGTTCGTTGCTTCCACTTGGGAAGATGGTGGACCGCCTCCTCATGCTAAATTTCTCGCTAATTGGCTTTCTGAAAGCGCGGAGGATTTTAGAGTTGGTTCGTTGTTGCTTTCTCAGTGTAAATTTGCTGTGTTTGGCGTTGGTAGTCGAGCTTATGGAGAGACATTCAATGCGGTTGCAATGGACTTTTCCAAGCGGATGAAGGCATTGGGTGCGAAGGAGATTTTAGCGGTTGGTGAAGGAGATATTGATGGAGGTGATATAGATAAGTATTTTGATGATTGGAGTCGGCAATTGCTGAAGGTTGTGAAGGTTGATGCTGTGGGAACTGGTGTAGATTTAGATTCTGGGATTGCTGGGGACAGTGATGCTGAAAGTGTTGAGGAGGATGAAGAAGAAGATACCGACCTCGGGGAGGAGGACATTGTTGATCTTGAGGATATTGCTGGTAAGGGTCCTTCGAGAAAGTCTACGAATGTAGTCGAAACTAATGGGAAATTGAATGGGAAAAAGGTTATGGTGACCCCGGTGATTAGAGCGAGCTTAGAAAAGCAGGGATATAAAATTATTGGCTCACATAGCGGAGTGAAAATATGTAGATGGACGAAGTCTCAGCTTCGAGGGCGAGGAGGTTGCTATAAACATTCATTTTATGGCATAGAAAGTCATCGATGCATGGAGGCAACTCCCAGTTTGGCTTGTGCCAACAAATGTGTTTTCTGCTGGAGGCATCACACAAATCCAGTAGGAAAGAGTTGGCAGTGGGAGATGGATGATCCATTGGATATTGTGAATTCTGCAATCGATTTACATACAAAAATGATCAAGCAAATGAAAGGTGTTCCAGGTGTTACGCAGGAGAAATTGGACGAAGGCCTGTCCCCAAGACATTGTGCCTTGTCTCTTGTTGGTGAACCTATTATGTACCCAGAGATTAATACACTTGTTGATGAATTGCATCGAAGGCGGATTTCAACTTTCTTGGTAACGAATGCTCAGTTTCCTGATAAAATAAAACTGCTGAAGCCAGTCACCCAGCTGTATGTAAGTGTAGATGCTGCGACGAAGGAGAGCTTGAAGGCAATTGATAGACCACTTTTTGGAGACTTCTGGGAGCGATTCATTGATTCCTTGAAAGCTCTAAATGATAAGCAACAGCGAACAGTTATTAATGATTTATGTGCCTCCATAATGTTGCAGGATTCCTTGAAAGCTCTAAATGACAAGCAACAGCGAACAGTTTATCGATTGACGTTGGTAAAAGGGTGGAACACAGAAGATATTGATGCCTATTCGAATCTCTTTAGCATTGGTAGACCTGATTTTGTGGAAATCAAAGGCGTCACATATTGTGGATCGTCTAATACTTCAAAGCTGACAATGGAGAACGTTCCTTGGCATTCTGACGTCAAGTCTTTTTCCGAGGCTTTAGCTGCAAAAAGTCGTGGGGAGTATGAAGTTGCTTGTGAGCATGTGCACTCATGTTGCGTTCTTTTGGCTAAAACTGACAAATTTAAGGTCGATGGCCAGTGGTTCACTTGGATAGATTATGAGAAATTCCATGACCTGGTGGCCTCTGGAAAACCTTTTGATAGCAAGGATTACATGGCTCCAACACCATCATGGGCTGTATATGGCTCTGACGAAGGTGGATTTGATCCTGATCAATCTCGGTACCGGAAGGAGCGACATCATAGACCAAAACCCACATCCTGATAGCTTTGAATGTCCGGGATTATTCCGAAGCAAAGCTTGTCAATGCCAACTCCATTGGATCAGTTCGAATGCAACCGGTGGAGAAAAAAGTACTCTTATCGGTTGGGAGTGGCAAGGCAGTGAAGAGTGTGCTGGAGGTGCTGGTAAATAACCCTGGGTAAAAGATGGAGAGACAAGATCCTCATCTGCTGGTCATTTGTCGCCAGGTAAATTTATTTGGTGATATGTGAAGAAATTGACCTTAGAACATTGTTTCTTCTATTATTTGGTAGTTTGTTTTAGGGAGGTCTACATACCATGAACCAAAGAGCAAGGATGACCATGGATGGATTTAGATGTATTTGAAAGGCTTAGGAATTCAGAATTAGTGTGTTGTTTTGTAGGAT

Coding sequence (CDS)

ATGTCCTTTTCTTCACGCTCCGCGAAGCTCACCCTTCTAGCTCTTCTCTCTGCCTCCACTTTGTATTGTTTCTACAAGTCCCGCCGTCTCAGAAAGCTTAAACTCTCCTTAAACCCTACTCCTTGTTCTCGCAGACCCAAACTGTTTTTTATTTCTCAAACTGGTACTTCCAAAGCCCTAGCTCATCGTCTGCTTAACGTTTTGAATTCCAATGGGTTGGTCTTTGATCTTGTCGATCCCAAGGATTATGAACCTGAAGATCTGTTTAAGGAGATTCTAGTTGTGTTCGTTGCTTCCACTTGGGAAGATGGTGGACCGCCTCCTCATGCTAAATTTCTCGCTAATTGGCTTTCTGAAAGCGCGGAGGATTTTAGAGTTGGTTCGTTGTTGCTTTCTCAGTGTAAATTTGCTGTGTTTGGCGTTGGTAGTCGAGCTTATGGAGAGACATTCAATGCGGTTGCAATGGACTTTTCCAAGCGGATGAAGGCATTGGGTGCGAAGGAGATTTTAGCGGTTGGTGAAGGAGATATTGATGGAGGTGATATAGATAAGTATTTTGATGATTGGAGTCGGCAATTGCTGAAGGTTGTGAAGGTTGATGCTGTGGGAACTGGTGTAGATTTAGATTCTGGGATTGCTGGGGACAGTGATGCTGAAAGTGTTGAGGAGGATGAAGAAGAAGATACCGACCTCGGGGAGGAGGACATTGTTGATCTTGAGGATATTGCTGGTAAGGGTCCTTCGAGAAAGTCTACGAATGTAGTCGAAACTAATGGGAAATTGAATGGGAAAAAGGTTATGGTGACCCCGGTGATTAGAGCGAGCTTAGAAAAGCAGGGATATAAAATTATTGGCTCACATAGCGGAGTGAAAATATGTAGATGGACGAAGTCTCAGCTTCGAGGGCGAGGAGGTTGCTATAAACATTCATTTTATGGCATAGAAAGTCATCGATGCATGGAGGCAACTCCCAGTTTGGCTTGTGCCAACAAATGTGTTTTCTGCTGGAGGCATCACACAAATCCAGTAGGAAAGAGTTGGCAGTGGGAGATGGATGATCCATTGGATATTGTGAATTCTGCAATCGATTTACATACAAAAATGATCAAGCAAATGAAAGGTGTTCCAGGTGTTACGCAGGAGAAATTGGACGAAGGCCTGTCCCCAAGACATTGTGCCTTGTCTCTTGTTGGTGAACCTATTATGTACCCAGAGATTAATACACTTGTTGATGAATTGCATCGAAGGCGGATTTCAACTTTCTTGGTAACGAATGCTCAGTTTCCTGATAAAATAAAACTGCTGAAGCCAGTCACCCAGCTGTATGTAAGTGTAGATGCTGCGACGAAGGAGAGCTTGAAGGCAATTGATAGACCACTTTTTGGAGACTTCTGGGAGCGATTCATTGATTCCTTGAAAGCTCTAAATGATAAGCAACAGCGAACAGTTATTAATGATTTATGTGCCTCCATAATGTTGCAGGATTCCTTGAAAGCTCTAAATGACAAGCAACAGCGAACAGTTTATCGATTGACGTTGGTAAAAGGGTGGAACACAGAAGATATTGATGCCTATTCGAATCTCTTTAGCATTGGTAGACCTGATTTTGTGGAAATCAAAGGCGTCACATATTGTGGATCGTCTAATACTTCAAAGCTGACAATGGAGAACGTTCCTTGGCATTCTGACGTCAAGTCTTTTTCCGAGGCTTTAGCTGCAAAAAGTCGTGGGGAGTATGAAGTTGCTTGTGAGCATGTGCACTCATGTTGCGTTCTTTTGGCTAAAACTGACAAATTTAAGGTCGATGGCCAGTGGTTCACTTGGATAGATTATGAGAAATTCCATGACCTGGTGGCCTCTGGAAAACCTTTTGATAGCAAGGATTACATGGCTCCAACACCATCATGGGCTGTATATGGCTCTGACGAAGGTGGATTTGATCCTGATCAATCTCGGTACCGGAAGGAGCGACATCATAGACCAAAACCCACATCCTGA

Protein sequence

MSFSSRSAKLTLLALLSASTLYCFYKSRRLRKLKLSLNPTPCSRRPKLFFISQTGTSKALAHRLLNVLNSNGLVFDLVDPKDYEPEDLFKEILVVFVASTWEDGGPPPHAKFLANWLSESAEDFRVGSLLLSQCKFAVFGVGSRAYGETFNAVAMDFSKRMKALGAKEILAVGEGDIDGGDIDKYFDDWSRQLLKVVKVDAVGTGVDLDSGIAGDSDAESVEEDEEEDTDLGEEDIVDLEDIAGKGPSRKSTNVVETNGKLNGKKVMVTPVIRASLEKQGYKIIGSHSGVKICRWTKSQLRGRGGCYKHSFYGIESHRCMEATPSLACANKCVFCWRHHTNPVGKSWQWEMDDPLDIVNSAIDLHTKMIKQMKGVPGVTQEKLDEGLSPRHCALSLVGEPIMYPEINTLVDELHRRRISTFLVTNAQFPDKIKLLKPVTQLYVSVDAATKESLKAIDRPLFGDFWERFIDSLKALNDKQQRTVINDLCASIMLQDSLKALNDKQQRTVYRLTLVKGWNTEDIDAYSNLFSIGRPDFVEIKGVTYCGSSNTSKLTMENVPWHSDVKSFSEALAAKSRGEYEVACEHVHSCCVLLAKTDKFKVDGQWFTWIDYEKFHDLVASGKPFDSKDYMAPTPSWAVYGSDEGGFDPDQSRYRKERHHRPKPTS

Homology

[Close]

BLAST of Cla97C08G158680 vs. NCBI nr
Match: XP_038884961.1 (S-adenosyl-L-methionine-dependent tRNA 4-demethylwyosine synthase [Benincasa hispida] >XP_038884963.1 S-adenosyl-L-methionine-dependent tRNA 4-demethylwyosine synthase [Benincasa hispida])

HSP 1 Score: 1252.3 bits (3239), Expect = 0.0e+00
Identity = 620/665 (93.23%), Postives = 630/665 (94.74%), Query Frame = 0


Query: 1   MSFSSRSAKLTLLALLSASTLYCFYKSRRLRKLKLSLNPTPCSRRPKLFFISQTGTSKAL 60
           MSFSSRSAKLTLLALLSASTLYCFYKSRRLRKLKLSLNPT  SRRPKLFFISQTGTSKAL
Sbjct: 1   MSFSSRSAKLTLLALLSASTLYCFYKSRRLRKLKLSLNPTLSSRRPKLFFISQTGTSKAL 60

Query: 61  AHRLLNVLNSNGLVFDLVDPKDYEPEDLFKEILVVFVASTWEDGGPPPHAKFLANWLSES 120
           AHRLLNVLNSN LVFDLVDPKDYEPEDLFKE LV+FVASTWEDGGPPPHAKFLANWLSES
Sbjct: 61  AHRLLNVLNSNELVFDLVDPKDYEPEDLFKETLVLFVASTWEDGGPPPHAKFLANWLSES 120

Query: 121 AEDFRVGSLLLSQCKFAVFGVGSRAYGETFNAVAMDFSKRMKALGAKEILAVGEGDIDGG 180
           AEDFRVGSLLLS+CKFAVFGVGSRAYGETFNAVA +FSKRMKALGAKEILAVG+GD+DGG
Sbjct: 121 AEDFRVGSLLLSECKFAVFGVGSRAYGETFNAVARNFSKRMKALGAKEILAVGKGDVDGG 180

Query: 181 DIDKYFDDWSRQLLKVVKVDAVGTGVDLDSGIAGDSDAESVEEDEEEDTDLGEEDIVDLE 240
           DID+ FDDWSRQLLKV+KVDAVG GVDL SGI GDSDAESVEEDEEED DLGEEDIVDLE
Sbjct: 181 DIDRCFDDWSRQLLKVLKVDAVGNGVDLYSGIGGDSDAESVEEDEEEDADLGEEDIVDLE 240

Query: 241 DIAGKGPSRKSTNVVETNGKLNGKKVMVTPVIRASLEKQGYKIIGSHSGVKICRWTKSQL 300
           DIAGKGPSRKSTNVVETNGKLNGKKVMVTPVIRASLEKQGYKIIGSHSGVKICRWTKSQL
Sbjct: 241 DIAGKGPSRKSTNVVETNGKLNGKKVMVTPVIRASLEKQGYKIIGSHSGVKICRWTKSQL 300

Query: 301 RGRGGCYKHSFYGIESHRCMEATPSLACANKCVFCWRHHTNPVGKSWQWEMDDPLDIVNS 360
           RGRGGCYKHSFYGIESHRCMEATPSLACANKCVFCWRHHTNPVGKSWQWEMDDPLDIVNS
Sbjct: 301 RGRGGCYKHSFYGIESHRCMEATPSLACANKCVFCWRHHTNPVGKSWQWEMDDPLDIVNS 360

Query: 361 AIDLHTKMIKQMKGVPGVTQEKLDEGLSPRHCALSLVGEPIMYPEINTLVDELHRRRIST 420
           AIDLHTKMIKQMKGVPGVTQEKLDEGLSPRHCALSLVGEPIMYPEINTLVDELHRRRIST
Sbjct: 361 AIDLHTKMIKQMKGVPGVTQEKLDEGLSPRHCALSLVGEPIMYPEINTLVDELHRRRIST 420

Query: 421 FLVTNAQFPDKIKLLKPVTQLYVSVDAATKESLKAIDRPLFGDFWERFIDSLKALNDKQQ 480
           FLVTNAQFPDKIKLLKPVTQLYVSVDAATKESLKAIDRPLFGDFWERFI           
Sbjct: 421 FLVTNAQFPDKIKLLKPVTQLYVSVDAATKESLKAIDRPLFGDFWERFI----------- 480

Query: 481 RTVINDLCASIMLQDSLKALNDKQQRTVYRLTLVKGWNTEDIDAYSNLFSIGRPDFVEIK 540
                         DSLKALNDKQQRTVYRLTLVKGWNTEDIDAYSNLFSIGRPDFVEIK
Sbjct: 481 --------------DSLKALNDKQQRTVYRLTLVKGWNTEDIDAYSNLFSIGRPDFVEIK 540

Query: 541 GVTYCGSSNTSKLTMENVPWHSDVKSFSEALAAKSRGEYEVACEHVHSCCVLLAKTDKFK 600
           GVTYCGSSNTSKLTMENVPWHSDVKSFSEALAAKS+GEYEVACEHVHSCCVLLAKTDKF+
Sbjct: 541 GVTYCGSSNTSKLTMENVPWHSDVKSFSEALAAKSQGEYEVACEHVHSCCVLLAKTDKFR 600

Query: 601 VDGQWFTWIDYEKFHDLVASGKPFDSKDYMAPTPSWAVYGSDEGGFDPDQSRYRKERHHR 660
           VDGQWFTWIDYEKFHDLVASGKPFDSKDYMAPTPSWAVYGSDEGGFDP+QSRYRKERHHR
Sbjct: 601 VDGQWFTWIDYEKFHDLVASGKPFDSKDYMAPTPSWAVYGSDEGGFDPNQSRYRKERHHR 640

Query: 661 PKPTS 666
           PKPTS
Sbjct: 661 PKPTS 640

[Close]

BLAST of Cla97C08G158680 vs. NCBI nr
Match: KAA0065238.1 (S-adenosyl-L-methionine-dependent tRNA 4-demethylwyosine synthase [Cucumis melo var. makuwa])

HSP 1 Score: 1235.7 bits (3196), Expect = 0.0e+00
Identity = 611/666 (91.74%), Postives = 627/666 (94.14%), Query Frame = 0


Query: 1   MSFSSRSAKLTLLALLSASTLYCFYKSRRLRKLKLSLNPTPCSRRPKLFFISQTGTSKAL 60
           MSFSSRSAKLT+LALLSASTLYCFYKSRRLRKLKLSLNPT  SR+PKLFFISQTGTSKAL
Sbjct: 1   MSFSSRSAKLTILALLSASTLYCFYKSRRLRKLKLSLNPTLSSRKPKLFFISQTGTSKAL 60

Query: 61  AHRLLNVLNSNGLVFDLVDPKDYEPEDLFKEILVVFVASTWEDGGPPPHAKFLANWLSES 120
           AHRLLNVLNSNGLVFDLVDPKDYEPEDLFKE LV+FVASTWEDGGPPPHAKFLANWLSES
Sbjct: 61  AHRLLNVLNSNGLVFDLVDPKDYEPEDLFKETLVLFVASTWEDGGPPPHAKFLANWLSES 120

Query: 121 AEDFRVGSLLLSQCKFAVFGVGSRAYGETFNAVAMDFSKRMKALGAKEILAVGEGDIDGG 180
           AEDFRVGSLLLSQCKF+VFGVGSR YGET+NAVA DFSKRMKALGAKEIL +GEGD+DGG
Sbjct: 121 AEDFRVGSLLLSQCKFSVFGVGSRVYGETYNAVARDFSKRMKALGAKEILPIGEGDVDGG 180

Query: 181 DIDKYFDDWSRQLLKVVKVDAVGTGVDLDSGIAGDSDAESVEED-EEEDTDLGEEDIVDL 240
           DIDK FDDWSR+LLKVVKVDA G GV+L SGIAGDSDA SVEE+ EEED DLGEEDIVDL
Sbjct: 181 DIDKCFDDWSRKLLKVVKVDAEGNGVELCSGIAGDSDAVSVEEEYEEEDDDLGEEDIVDL 240

Query: 241 EDIAGKGPSRKSTNVVETNGKLNGKKVMVTPVIRASLEKQGYKIIGSHSGVKICRWTKSQ 300
           EDIAGKGPSRKSTNVVETNGKLNGKKVMVTPVIRASLEKQGYKIIGSHSGVKICRWTKSQ
Sbjct: 241 EDIAGKGPSRKSTNVVETNGKLNGKKVMVTPVIRASLEKQGYKIIGSHSGVKICRWTKSQ 300

Query: 301 LRGRGGCYKHSFYGIESHRCMEATPSLACANKCVFCWRHHTNPVGKSWQWEMDDPLDIVN 360
           LRGRGGCYKHSFYGIESHRCMEATPSLACANKCVFCWRHHTNPVGKSWQWEMDDPLDIVN
Sbjct: 301 LRGRGGCYKHSFYGIESHRCMEATPSLACANKCVFCWRHHTNPVGKSWQWEMDDPLDIVN 360

Query: 361 SAIDLHTKMIKQMKGVPGVTQEKLDEGLSPRHCALSLVGEPIMYPEINTLVDELHRRRIS 420
           SAIDLHTKMIKQMKGVPGVTQEKL+EGLSPRHCA SLVGEPIMYPEINTLVDELH+RRIS
Sbjct: 361 SAIDLHTKMIKQMKGVPGVTQEKLEEGLSPRHCAFSLVGEPIMYPEINTLVDELHQRRIS 420

Query: 421 TFLVTNAQFPDKIKLLKPVTQLYVSVDAATKESLKAIDRPLFGDFWERFIDSLKALNDKQ 480
           TFLVTNAQFPDKIKLLKPVTQLYVSVDAATKESLKAIDRPLFGDFWERFI          
Sbjct: 421 TFLVTNAQFPDKIKLLKPVTQLYVSVDAATKESLKAIDRPLFGDFWERFI---------- 480

Query: 481 QRTVINDLCASIMLQDSLKALNDKQQRTVYRLTLVKGWNTEDIDAYSNLFSIGRPDFVEI 540
                          DSLKALN+KQQRTVYRLTLVKGWNTEDIDAYSNLFSIGRPDFVEI
Sbjct: 481 ---------------DSLKALNEKQQRTVYRLTLVKGWNTEDIDAYSNLFSIGRPDFVEI 540

Query: 541 KGVTYCGSSNTSKLTMENVPWHSDVKSFSEALAAKSRGEYEVACEHVHSCCVLLAKTDKF 600
           KGVTYCGSSNTSKLTMENVPWHSDVKSFSEALAAKS+GEYEVACEHVHSCCVLLAKTDKF
Sbjct: 541 KGVTYCGSSNTSKLTMENVPWHSDVKSFSEALAAKSQGEYEVACEHVHSCCVLLAKTDKF 600

Query: 601 KVDGQWFTWIDYEKFHDLVASGKPFDSKDYMAPTPSWAVYGSDEGGFDPDQSRYRKERHH 660
           +VDGQWFTWIDYEKFHDLVASGKPFDSKDYMAPTPSWAVYGSDEGGFDPDQSRYRKERHH
Sbjct: 601 RVDGQWFTWIDYEKFHDLVASGKPFDSKDYMAPTPSWAVYGSDEGGFDPDQSRYRKERHH 641

Query: 661 RPKPTS 666
           +PKPTS
Sbjct: 661 KPKPTS 641

[Close]

BLAST of Cla97C08G158680 vs. NCBI nr
Match: XP_008444711.1 (PREDICTED: S-adenosyl-L-methionine-dependent tRNA 4-demethylwyosine synthase [Cucumis melo])

HSP 1 Score: 1234.6 bits (3193), Expect = 0.0e+00
Identity = 610/666 (91.59%), Postives = 628/666 (94.29%), Query Frame = 0


Query: 1   MSFSSRSAKLTLLALLSASTLYCFYKSRRLRKLKLSLNPTPCSRRPKLFFISQTGTSKAL 60
           MSFSSRSAKLT+LALLSASTLYCFYKSRRLRKLKLSLNPT  SR+PKLFFISQTGTSKAL
Sbjct: 1   MSFSSRSAKLTILALLSASTLYCFYKSRRLRKLKLSLNPTLSSRKPKLFFISQTGTSKAL 60

Query: 61  AHRLLNVLNSNGLVFDLVDPKDYEPEDLFKEILVVFVASTWEDGGPPPHAKFLANWLSES 120
           AHRLLNVLNSNGLVFDLVDPKDYEPEDLFKE LV+FVASTWEDGGPPPHAKFLANWLSES
Sbjct: 61  AHRLLNVLNSNGLVFDLVDPKDYEPEDLFKETLVLFVASTWEDGGPPPHAKFLANWLSES 120

Query: 121 AEDFRVGSLLLSQCKFAVFGVGSRAYGETFNAVAMDFSKRMKALGAKEILAVGEGDIDGG 180
           AEDFRVGSLLLSQCKF+VFGVGSR YGET+NAVA DFSKRMK+LGAKEIL +GEGD+DGG
Sbjct: 121 AEDFRVGSLLLSQCKFSVFGVGSRVYGETYNAVARDFSKRMKSLGAKEILPIGEGDVDGG 180

Query: 181 DIDKYFDDWSRQLLKVVKVDAVGTGVDLDSGIAGDSDAESVEED-EEEDTDLGEEDIVDL 240
           DIDK FDDWSR+LLKVVKVDA G GV+L SGIAGDSDA SVEE+ EEED DLGEEDIVDL
Sbjct: 181 DIDKCFDDWSRKLLKVVKVDAEGNGVELCSGIAGDSDAVSVEEEYEEEDDDLGEEDIVDL 240

Query: 241 EDIAGKGPSRKSTNVVETNGKLNGKKVMVTPVIRASLEKQGYKIIGSHSGVKICRWTKSQ 300
           EDIAGKGPSRKSTNVVETNGKLNGKKVMVTPVIRASLEKQGYKIIGSHSGVKICRWTKSQ
Sbjct: 241 EDIAGKGPSRKSTNVVETNGKLNGKKVMVTPVIRASLEKQGYKIIGSHSGVKICRWTKSQ 300

Query: 301 LRGRGGCYKHSFYGIESHRCMEATPSLACANKCVFCWRHHTNPVGKSWQWEMDDPLDIVN 360
           LRGRGGCYKHSFYGIESHRCMEATPSLACANKCVFCWRHHTNPVGKSWQWEMDDPLDIVN
Sbjct: 301 LRGRGGCYKHSFYGIESHRCMEATPSLACANKCVFCWRHHTNPVGKSWQWEMDDPLDIVN 360

Query: 361 SAIDLHTKMIKQMKGVPGVTQEKLDEGLSPRHCALSLVGEPIMYPEINTLVDELHRRRIS 420
           SAIDLHTKMIKQMKGVPGVTQEKL+EGLSPRHCALSLVGEPIMYPEINTLVDELH+RRIS
Sbjct: 361 SAIDLHTKMIKQMKGVPGVTQEKLEEGLSPRHCALSLVGEPIMYPEINTLVDELHQRRIS 420

Query: 421 TFLVTNAQFPDKIKLLKPVTQLYVSVDAATKESLKAIDRPLFGDFWERFIDSLKALNDKQ 480
           TFLVTNAQFPDKIKLLKPVTQLYVSVDAATKESLKAIDRPLFGDFWERFI          
Sbjct: 421 TFLVTNAQFPDKIKLLKPVTQLYVSVDAATKESLKAIDRPLFGDFWERFI---------- 480

Query: 481 QRTVINDLCASIMLQDSLKALNDKQQRTVYRLTLVKGWNTEDIDAYSNLFSIGRPDFVEI 540
                          DSLKALN+KQQRTVYRLTLVKGWNTEDIDAYSNLFSIGRPDFVEI
Sbjct: 481 ---------------DSLKALNEKQQRTVYRLTLVKGWNTEDIDAYSNLFSIGRPDFVEI 540

Query: 541 KGVTYCGSSNTSKLTMENVPWHSDVKSFSEALAAKSRGEYEVACEHVHSCCVLLAKTDKF 600
           KGVTYCGSSNTSKLTMENVPWHSDVKSFSEALAAKS+GEYEVACEHVHSCCVLLAKTDKF
Sbjct: 541 KGVTYCGSSNTSKLTMENVPWHSDVKSFSEALAAKSQGEYEVACEHVHSCCVLLAKTDKF 600

Query: 601 KVDGQWFTWIDYEKFHDLVASGKPFDSKDYMAPTPSWAVYGSDEGGFDPDQSRYRKERHH 660
           +VDGQWFTWIDYEKFHDLVASGKPFDSKDY+APTPSWAVYGSDEGGFDPDQSRYRKERHH
Sbjct: 601 RVDGQWFTWIDYEKFHDLVASGKPFDSKDYIAPTPSWAVYGSDEGGFDPDQSRYRKERHH 641

Query: 661 RPKPTS 666
           +PKPTS
Sbjct: 661 KPKPTS 641

[Close]

BLAST of Cla97C08G158680 vs. NCBI nr
Match: XP_004152698.1 (S-adenosyl-L-methionine-dependent tRNA 4-demethylwyosine synthase [Cucumis sativus] >KGN62597.1 hypothetical protein Csa_021988 [Cucumis sativus])

HSP 1 Score: 1233.4 bits (3190), Expect = 0.0e+00
Identity = 607/665 (91.28%), Postives = 625/665 (93.98%), Query Frame = 0


Query: 1   MSFSSRSAKLTLLALLSASTLYCFYKSRRLRKLKLSLNPTPCSRRPKLFFISQTGTSKAL 60
           MS SSRSAKLT+LALLSASTLYCFYKSRRLRKLKLSLNPT  SR+PKLFFISQTGTSKAL
Sbjct: 1   MSLSSRSAKLTILALLSASTLYCFYKSRRLRKLKLSLNPTLPSRKPKLFFISQTGTSKAL 60

Query: 61  AHRLLNVLNSNGLVFDLVDPKDYEPEDLFKEILVVFVASTWEDGGPPPHAKFLANWLSES 120
           AHRLLNVLNSNGLVFDLVDPKDYEPEDLFKE LV+FVASTWEDGGPPPHAKFLANWLSES
Sbjct: 61  AHRLLNVLNSNGLVFDLVDPKDYEPEDLFKETLVLFVASTWEDGGPPPHAKFLANWLSES 120

Query: 121 AEDFRVGSLLLSQCKFAVFGVGSRAYGETFNAVAMDFSKRMKALGAKEILAVGEGDIDGG 180
           AEDFRVGSLLLSQCKF+VFGVGSR YGETFNAVA DFSKR+KALGAKEIL +GEGD+DGG
Sbjct: 121 AEDFRVGSLLLSQCKFSVFGVGSRVYGETFNAVARDFSKRLKALGAKEILPIGEGDVDGG 180

Query: 181 DIDKYFDDWSRQLLKVVKVDAVGTGVDLDSGIAGDSDAESVEEDEEEDTDLGEEDIVDLE 240
           DIDK FDDWSR+LLKV+K DA G GV+L SGIAGDSDA SVEEDEEED DLGEEDIVDLE
Sbjct: 181 DIDKCFDDWSRKLLKVMKFDAEGNGVELCSGIAGDSDAVSVEEDEEEDDDLGEEDIVDLE 240

Query: 241 DIAGKGPSRKSTNVVETNGKLNGKKVMVTPVIRASLEKQGYKIIGSHSGVKICRWTKSQL 300
           DIAGKGPSRKSTN+VETNGKLNGKKVMVTPVIRASLEKQGYKIIGSHSGVKICRWTKSQL
Sbjct: 241 DIAGKGPSRKSTNIVETNGKLNGKKVMVTPVIRASLEKQGYKIIGSHSGVKICRWTKSQL 300

Query: 301 RGRGGCYKHSFYGIESHRCMEATPSLACANKCVFCWRHHTNPVGKSWQWEMDDPLDIVNS 360
           RGRGGCYKHSFYGIESHRCMEATPSLACANKCVFCWRHHTNPVGKSWQWEMDDPL+IVNS
Sbjct: 301 RGRGGCYKHSFYGIESHRCMEATPSLACANKCVFCWRHHTNPVGKSWQWEMDDPLEIVNS 360

Query: 361 AIDLHTKMIKQMKGVPGVTQEKLDEGLSPRHCALSLVGEPIMYPEINTLVDELHRRRIST 420
           AIDLHTKMIKQMKGVPGVTQEKL+EGLSPRHCALSLVGEPIMYPEINTLVDELHRRRIST
Sbjct: 361 AIDLHTKMIKQMKGVPGVTQEKLEEGLSPRHCALSLVGEPIMYPEINTLVDELHRRRIST 420

Query: 421 FLVTNAQFPDKIKLLKPVTQLYVSVDAATKESLKAIDRPLFGDFWERFIDSLKALNDKQQ 480
           FLVTNAQFPDKIKLLKPVTQLYVSVDAATKESLKAIDRPLFGDFWERFI           
Sbjct: 421 FLVTNAQFPDKIKLLKPVTQLYVSVDAATKESLKAIDRPLFGDFWERFI----------- 480

Query: 481 RTVINDLCASIMLQDSLKALNDKQQRTVYRLTLVKGWNTEDIDAYSNLFSIGRPDFVEIK 540
                         DSLKALN+KQQRTVYRLTLVKGWNTEDIDAYSNLFSIGRPDFVEIK
Sbjct: 481 --------------DSLKALNEKQQRTVYRLTLVKGWNTEDIDAYSNLFSIGRPDFVEIK 540

Query: 541 GVTYCGSSNTSKLTMENVPWHSDVKSFSEALAAKSRGEYEVACEHVHSCCVLLAKTDKFK 600
           GVTYCGSS TSKLTMENVPWHSDVKSFSEALAAKS+GEYEVACEHVHSCCVLLAKTDKF+
Sbjct: 541 GVTYCGSSTTSKLTMENVPWHSDVKSFSEALAAKSQGEYEVACEHVHSCCVLLAKTDKFR 600

Query: 601 VDGQWFTWIDYEKFHDLVASGKPFDSKDYMAPTPSWAVYGSDEGGFDPDQSRYRKERHHR 660
           VDGQWFTWIDY+KFHDLVASGKPFDSKDYMAPTPSWAVYGSDEGGFDPDQSRYRKERHH+
Sbjct: 601 VDGQWFTWIDYDKFHDLVASGKPFDSKDYMAPTPSWAVYGSDEGGFDPDQSRYRKERHHK 640

Query: 661 PKPTS 666
           PKPTS
Sbjct: 661 PKPTS 640

[Close]

BLAST of Cla97C08G158680 vs. NCBI nr
Match: XP_022997485.1 (S-adenosyl-L-methionine-dependent tRNA 4-demethylwyosine synthase [Cucurbita maxima])

HSP 1 Score: 1232.2 bits (3187), Expect = 0.0e+00
Identity = 611/665 (91.88%), Postives = 623/665 (93.68%), Query Frame = 0


Query: 1   MSFSSRSAKLTLLALLSASTLYCFYKSRRLRKLKLSLNPTPCSRRPKLFFISQTGTSKAL 60
           MSFSSRSAKL LLALLSASTLYCFYKSRRLRKLKLSLNPT CSRRPKLFFISQTGTSKAL
Sbjct: 1   MSFSSRSAKLALLALLSASTLYCFYKSRRLRKLKLSLNPTGCSRRPKLFFISQTGTSKAL 60

Query: 61  AHRLLNVLNSNGLVFDLVDPKDYEPEDLFKEILVVFVASTWEDGGPPPHAKFLANWLSES 120
           AHRLLNVLNSN L FD+VDPKD+EPEDLFKE LV+FVASTWEDGGPPPHAKFLANWLSES
Sbjct: 61  AHRLLNVLNSNELAFDIVDPKDFEPEDLFKEALVLFVASTWEDGGPPPHAKFLANWLSES 120

Query: 121 AEDFRVGSLLLSQCKFAVFGVGSRAYGETFNAVAMDFSKRMKALGAKEILAVGEGDIDGG 180
           AEDFRVGSLLLSQCKFAVFGVGSRAYGETFNAVA DFSKRMKALGAKEILAVGEGD+DGG
Sbjct: 121 AEDFRVGSLLLSQCKFAVFGVGSRAYGETFNAVARDFSKRMKALGAKEILAVGEGDVDGG 180

Query: 181 DIDKYFDDWSRQLLKVVKVDAVGTGVDLDSGIAGDSDAESVEEDEEEDTDLGEEDIVDLE 240
           DIDK FDDWSRQLLKV+KVDAVGTG DL SGI  D DAESVEED +ED D GEEDIVDLE
Sbjct: 181 DIDKCFDDWSRQLLKVLKVDAVGTGADLYSGIDEDFDAESVEED-DEDADPGEEDIVDLE 240

Query: 241 DIAGKGPSRKSTNVVETNGKLNGKKVMVTPVIRASLEKQGYKIIGSHSGVKICRWTKSQL 300
           DIAGKGPSRKSTNVVETNGKLNGKKVMVTPVIRASLEKQGYKIIGSHSGVKICRWTKSQL
Sbjct: 241 DIAGKGPSRKSTNVVETNGKLNGKKVMVTPVIRASLEKQGYKIIGSHSGVKICRWTKSQL 300

Query: 301 RGRGGCYKHSFYGIESHRCMEATPSLACANKCVFCWRHHTNPVGKSWQWEMDDPLDIVNS 360
           RGRGGCYKHSFYGIESHRCMEATPSLACANKCVFCWRHHTNPVGKSWQWEMDDPLDIVNS
Sbjct: 301 RGRGGCYKHSFYGIESHRCMEATPSLACANKCVFCWRHHTNPVGKSWQWEMDDPLDIVNS 360

Query: 361 AIDLHTKMIKQMKGVPGVTQEKLDEGLSPRHCALSLVGEPIMYPEINTLVDELHRRRIST 420
           AIDLHTKMIKQMKGVPGVTQEKL+EGLSPRHCALSLVGEPIMYPEINTLVDELHRRRIST
Sbjct: 361 AIDLHTKMIKQMKGVPGVTQEKLEEGLSPRHCALSLVGEPIMYPEINTLVDELHRRRIST 420

Query: 421 FLVTNAQFPDKIKLLKPVTQLYVSVDAATKESLKAIDRPLFGDFWERFIDSLKALNDKQQ 480
           FLVTNAQFPDKIK LKPVTQLYVSVDAATKESLKAIDRPLFGDFWERFI           
Sbjct: 421 FLVTNAQFPDKIKSLKPVTQLYVSVDAATKESLKAIDRPLFGDFWERFI----------- 480

Query: 481 RTVINDLCASIMLQDSLKALNDKQQRTVYRLTLVKGWNTEDIDAYSNLFSIGRPDFVEIK 540
                         DSLKALNDKQQRTVYRLTLVKGWNTEDIDAYSNLFS+G+PDFVEIK
Sbjct: 481 --------------DSLKALNDKQQRTVYRLTLVKGWNTEDIDAYSNLFSLGKPDFVEIK 540

Query: 541 GVTYCGSSNTSKLTMENVPWHSDVKSFSEALAAKSRGEYEVACEHVHSCCVLLAKTDKFK 600
           GVTYCGSSNTSKLTMENVPWHSDVKSFSEALAAKS+GEYEVACEHVHSCCVLLAKTDKF+
Sbjct: 541 GVTYCGSSNTSKLTMENVPWHSDVKSFSEALAAKSQGEYEVACEHVHSCCVLLAKTDKFR 600

Query: 601 VDGQWFTWIDYEKFHDLVASGKPFDSKDYMAPTPSWAVYGSDEGGFDPDQSRYRKERHHR 660
           VDGQWFTWIDYEKFHDLVASGKPFDSKDYMAPTPSWAVYG+DEGGFDP QSRYRKERHHR
Sbjct: 601 VDGQWFTWIDYEKFHDLVASGKPFDSKDYMAPTPSWAVYGADEGGFDPVQSRYRKERHHR 639

Query: 661 PKPTS 666
           PKPTS
Sbjct: 661 PKPTS 639

[Close]

BLAST of Cla97C08G158680 vs. ExPASy Swiss-Prot
Match: Q8RXN5 (S-adenosyl-L-methionine-dependent tRNA 4-demethylwyosine synthase OS=Arabidopsis thaliana OX=3702 GN=TYW1 PE=2 SV=1)

HSP 1 Score: 937.6 bits (2422), Expect = 7.9e-272
Identity = 473/669 (70.70%), Postives = 534/669 (79.82%), Query Frame = 0


Query: 4   SSRSAKLTLLALLSASTLYCFYKSRRLRKLK-LSLNP--TPCSRRPKLFFISQTGTSKAL 63
           SS   +L  +ALLSA+T YC +K RRL+ LK LSLNP  T  + R K+FFISQTGT+KAL
Sbjct: 5   SSVRVRLAFVALLSATTFYCIHKYRRLKHLKNLSLNPSSTLKASRGKIFFISQTGTAKAL 64

Query: 64  AHRLLNVLNSNGLVFDLVDPKDYEPEDLFKEILVVFVASTWEDGGPPPHAKFLANWLSES 123
           A RL  +  SN + FD+VDP  YEPEDL KE LV+F+ASTW+ G PP + +FL NWL ES
Sbjct: 65  AQRLHELCASNDIAFDIVDPHSYEPEDLPKETLVLFIASTWDGGKPPKNGEFLVNWLGES 124

Query: 124 AEDFRVGSLLLSQCKFAVFGVGSRAYGETFNAVAMDFSKRMKALGAKEILAVGEGDIDGG 183
           AEDFRVGSLLLS CKFAVFGVGSRAYGE++NAVA + S RM  LG  E++ VGEGD+D G
Sbjct: 125 AEDFRVGSLLLSDCKFAVFGVGSRAYGESYNAVAKELSSRMIGLGGLEMIPVGEGDVDDG 184

Query: 184 DIDKYFDDWSRQLLKVVKVDAV--GTGVDLDSGIAGDSDAE----SVEEDEEEDTDLGEE 243
           ++D+ F DW   +++V+K  +     GV    G A + D E    + EEDE+ D D G  
Sbjct: 185 ELDRAFQDWCDGVIRVLKGGSAQETNGVSQQIG-AVEDDLEYYDSTDEEDEDNDADGG-- 244

Query: 244 DIVDLEDIAGKGPSRKSTNVVETNGKLNGKKVMVTPVIRASLEKQGYKIIGSHSGVKICR 303
            IVDLEDIAGK PS+++  V  T  K++GKK MVTPVIRASL KQGYKIIGSHSGVKICR
Sbjct: 245 -IVDLEDIAGKAPSKRNGVVKVT--KVDGKKEMVTPVIRASLTKQGYKIIGSHSGVKICR 304

Query: 304 WTKSQLRGRGGCYKHSFYGIESHRCMEATPSLACANKCVFCWRHHTNPVGKSWQWEMDDP 363
           WTKSQLRGRGGCYKHSFYGIESHRCME TPSLACANKCVFCWRHHTNPVGKSWQW+MD+P
Sbjct: 305 WTKSQLRGRGGCYKHSFYGIESHRCMETTPSLACANKCVFCWRHHTNPVGKSWQWKMDEP 364

Query: 364 LDIVNSAIDLHTKMIKQMKGVPGVTQEKLDEGLSPRHCALSLVGEPIMYPEINTLVDELH 423
             IV  A+DLH  MIKQMKGVPGVT EKL EGL+PRHCALSLVGEPIMYPEIN LVDELH
Sbjct: 365 SVIVKGALDLHKNMIKQMKGVPGVTPEKLQEGLNPRHCALSLVGEPIMYPEINALVDELH 424

Query: 424 RRRISTFLVTNAQFPDKIKLLKPVTQLYVSVDAATKESLKAIDRPLFGDFWERFIDSLKA 483
            RRISTFLVTNAQFP+KI ++KP+TQLYVSVDAATKESLKAIDRPLF DFWERFI     
Sbjct: 425 GRRISTFLVTNAQFPEKILMMKPITQLYVSVDAATKESLKAIDRPLFADFWERFI----- 484

Query: 484 LNDKQQRTVINDLCASIMLQDSLKALNDKQQRTVYRLTLVKGWNTEDIDAYSNLFSIGRP 543
                               DSLKAL +KQQRTVYRLTLVKGWNTE++DAY NLFSIG+P
Sbjct: 485 --------------------DSLKALQEKQQRTVYRLTLVKGWNTEELDAYFNLFSIGKP 544

Query: 544 DFVEIKGVTYCGSSNTSKLTMENVPWHSDVKSFSEALAAKSRGEYEVACEHVHSCCVLLA 603
           DF+EIKGVTYCGSS TSKLTMENVPWH+DVK+FSEAL+ KS GEYEVACEH HSCCVLL 
Sbjct: 545 DFIEIKGVTYCGSSATSKLTMENVPWHTDVKAFSEALSLKSNGEYEVACEHAHSCCVLLG 604

Query: 604 KTDKFKVDGQWFTWIDYEKFHDLVASGKPFDSKDYMAPTPSWAVYGSDEGGFDPDQSRYR 663
           +T+KFKVDG+WFTWIDYEKFHDLVASG+PF S DYMA TPSWAVYG+ EGGFDP Q RY+
Sbjct: 605 RTEKFKVDGKWFTWIDYEKFHDLVASGEPFTSTDYMAQTPSWAVYGAQEGGFDPGQLRYK 642

[Close]

BLAST of Cla97C08G158680 vs. ExPASy Swiss-Prot
Match: Q8H8N3 (S-adenosyl-L-methionine-dependent tRNA 4-demethylwyosine synthase OS=Oryza sativa subsp. japonica OX=39947 GN=TYW1 PE=2 SV=1)

HSP 1 Score: 874.4 bits (2258), Expect = 8.2e-253
Identity = 440/664 (66.27%), Postives = 515/664 (77.56%), Query Frame = 0


Query: 4   SSRSAKLTLLALLSASTLYCFYKSRRLRKLKLSLNPTP---CSRRPKLFFISQTGTSKAL 63
           +S ++ L LL LLS+S+++  YKS RLR+     +P P    +  P L + S TGTSKAL
Sbjct: 12  ASTTSHLALLLLLSSSSVFFLYKSLRLRR-NNPPSPPPGQGPAPTPTLLYASATGTSKAL 71

Query: 64  AHRLLNVLNSNGLVFDLVDPKDYEPEDLFKEILVVFVASTWEDGGPPPHAKFLANWLSES 123
           A  L   L   G+     D   ++P+DL    L++ V  T + G PPP A FLA WL ES
Sbjct: 72  AAGLSRRLAEAGVTAHPADAAAFDPDDLPSLPLLLLVLPTHDGGAPPPAAAFLARWLEES 131

Query: 124 AEDFRVGSLLLSQCKFAVFGVGSRAYGETFNAVAMDFSKRMKALGAKEILAVGEGDIDGG 183
           A DFR G+ LLS  +FAVFGVGSRAYGETFNA A  FS+ ++ALGA E++AVGEGD+DGG
Sbjct: 132 AADFRAGAALLSGLRFAVFGVGSRAYGETFNAAARSFSRWLRALGAAEVVAVGEGDVDGG 191

Query: 184 DIDKYFDDWSRQLLKVVKVDAVGTGVDLDS-GIAGDSDAESVEEDEEEDTDLGEEDIVDL 243
           D++  F++W  ++++VVK + +G G + +S G     + ES ++D+EE+ D GE   VD+
Sbjct: 192 DLEVVFEEWCGRVVRVVKGEEIGEGHNGESDGFDELEEEESDDDDDEEEVDGGE---VDM 251

Query: 244 EDIAGKGP-SRKSTNVVE---TNGKLNGKKVMVTPVIRASLEKQGYKIIGSHSGVKICRW 303
           EDIAGK P +R+    VE   +NG  NG + MVTP+IR SLEKQGYKIIGSHSGVKICRW
Sbjct: 252 EDIAGKAPAARRRNGKVEGALSNGGENGVRDMVTPIIRTSLEKQGYKIIGSHSGVKICRW 311

Query: 304 TKSQLRGRGGCYKHSFYGIESHRCMEATPSLACANKCVFCWRHHTNPVGKSWQWEMDDPL 363
           TKSQLRGRGGCYKHSFYGIESHRCMEATPSLACANKCVFCWRHHTNPVGKSW+W+MDDPL
Sbjct: 312 TKSQLRGRGGCYKHSFYGIESHRCMEATPSLACANKCVFCWRHHTNPVGKSWKWKMDDPL 371

Query: 364 DIVNSAIDLHTKMIKQMKGVPGVTQEKLDEGLSPRHCALSLVGEPIMYPEINTLVDELHR 423
           DIVN+AID HTKM+KQMKGVPGV  E+L EGLSPRHCALSLVGEPIMYPEIN L+DELHR
Sbjct: 372 DIVNAAIDQHTKMVKQMKGVPGVKPERLAEGLSPRHCALSLVGEPIMYPEINVLIDELHR 431

Query: 424 RRISTFLVTNAQFPDKIKLLKPVTQLYVSVDAATKESLKAIDRPLFGDFWERFIDSLKAL 483
           R ISTFLVTNAQFPDKIK LKP+TQLYVSVDAATKESLKA+DRPLF DFWERF+      
Sbjct: 432 RHISTFLVTNAQFPDKIKTLKPITQLYVSVDAATKESLKAVDRPLFSDFWERFL------ 491

Query: 484 NDKQQRTVINDLCASIMLQDSLKALNDKQQRTVYRLTLVKGWNTEDIDAYSNLFSIGRPD 543
                              DSLK+L+DK QRTVYRLTLVKGWN E+ID Y+ L S+G+PD
Sbjct: 492 -------------------DSLKSLHDKDQRTVYRLTLVKGWNAEEIDGYAKLLSLGQPD 551

Query: 544 FVEIKGVTYCGSSNTSKLTMENVPWHSDVKSFSEALAAKSRGEYEVACEHVHSCCVLLAK 603
           F+EIKGVTYCGSS TSKLTMENVPWHSDVK FSEALA KS G YEVACEH HSCCVLLAK
Sbjct: 552 FIEIKGVTYCGSSATSKLTMENVPWHSDVKDFSEALALKSGGVYEVACEHAHSCCVLLAK 611

Query: 604 TDKFKVDGQWFTWIDYEKFHDLVASGKPFDSKDYMAPTPSWAVYGSDEGGFDPDQSRYRK 660
            DKFK++G+W TWIDY++FH+LV SGKPF S+DYMA TPSWAVYG++EGGFDPDQSRY+K
Sbjct: 612 VDKFKINGKWHTWIDYDRFHELVTSGKPFRSQDYMALTPSWAVYGAEEGGFDPDQSRYKK 646

[Close]

BLAST of Cla97C08G158680 vs. ExPASy Swiss-Prot
Match: Q2KHP8 (S-adenosyl-L-methionine-dependent tRNA 4-demethylwyosine synthase TYW1 OS=Xenopus laevis OX=8355 GN=tyw1 PE=2 SV=1)

HSP 1 Score: 604.0 bits (1556), Expect = 2.1e-171
Identity = 320/672 (47.62%), Postives = 419/672 (62.35%), Query Frame = 0


Query: 47  KLFFISQTGTSKALAHRLLNVLNSNGLVFDLVDPKDYEPEDLFKE-----ILVVFVASTW 106
           K+F+ SQTGTSK  AH L   +   GL  +L++ K+Y+P+D   E      + VF+ +T+
Sbjct: 84  KIFYGSQTGTSKGFAHLLAEEVTLLGLPVELINMKEYDPDDNLVEETTSKNICVFLVATY 143

Query: 107 EDGGPPPHAKFLANWLSESAEDFRVGSLLLSQCKFAVFGVGSRAYGETFNAVAMDFSKRM 166
            DG PP  A++   WL E++ DFR G   L   ++AVFG+G+  Y   +N V  +  K +
Sbjct: 144 TDGKPPESAEWFCKWLEEASNDFRFGKTYLKGMRYAVFGLGNSVYSTHYNTVGKNIDKWL 203

Query: 167 KALGAKEILAVGEGDID-----GGDIDKYFDDWSRQLLKVVKVDAVGTGVDLDSGI---- 226
             L A  ++   EGD +      G I+  F+ W R+ L  +K    G             
Sbjct: 204 WMLSANRVMTRAEGDCNVVKSKHGSIESDFEAWKRKFLNRLKALLSGEKKPCSGKCKKGK 263

Query: 227 ---AGDSDAESVEEDEEEDTDLGEED------------------------IVDLEDIAGK 286
                 S  ESVEE+EEE+    E +                        ++D+ED+ GK
Sbjct: 264 CKSKKKSSIESVEEEEEEEEKHSEHEDTEDDTFETSSDSEPEEHGEPGSGLIDVEDL-GK 323

Query: 287 GPS--RKSTNVVETNGKLNGKKV-------------MVTPVIRASLEKQGYKIIGSHSGV 346
             S  +KS    ++N +L GK +             M+TP ++ +L KQGY++IGSHSGV
Sbjct: 324 AMSNMKKSKREHDSNTEL-GKSLQGDEAKEKEEPREMITPALQEALTKQGYRLIGSHSGV 383

Query: 347 KICRWTKSQLRGRGGCYKHSFYGIESHRCMEATPSLACANKCVFCWRHHTNPVGKSWQWE 406
           K+CRWTKS LRGRGGCYKH+FYGIESHRCME TPSLACANKCVFCWRHHTNPVG  W+W+
Sbjct: 384 KLCRWTKSMLRGRGGCYKHTFYGIESHRCMETTPSLACANKCVFCWRHHTNPVGTEWRWK 443

Query: 407 MDDPLDIVNSAIDLHTKMIKQMKGVPGVTQEKLDEGLSPRHCALSLVGEPIMYPEINTLV 466
           MD P  I+  AI  H  MIKQ KGVPGV  E+ +EGL+ +HCALSLVGEPIMYPEINT +
Sbjct: 444 MDQPEMILEEAILNHQNMIKQFKGVPGVKPERFEEGLAVKHCALSLVGEPIMYPEINTFL 503

Query: 467 DELHRRRISTFLVTNAQFPDKIKLLKPVTQLYVSVDAATKESLKAIDRPLFGDFWERFID 526
             LH + IS+FLVTNAQFP++I+ L+PVTQLYVSVDA+TK+SLK IDRPLF DFW+RFI 
Sbjct: 504 RLLHNQHISSFLVTNAQFPEEIRSLEPVTQLYVSVDASTKDSLKKIDRPLFKDFWQRFI- 563

Query: 527 SLKALNDKQQRTVINDLCASIMLQDSLKALNDKQQRTVYRLTLVKGWNTEDIDAYSNLFS 586
                                   DSLKAL +KQQRTVYRLTLVK WN +++ AY++L +
Sbjct: 564 ------------------------DSLKALAEKQQRTVYRLTLVKAWNVDELKAYADLVA 623

Query: 587 IGRPDFVEIKGVTYCGSSNTSKLTMENVPWHSDVKSFSEALAAKSRGEYEVACEHVHSCC 646
           +G PDF+E+KGVTYCG S+ S LTM NVPWH +V  F E L      +YEVACEH HS C
Sbjct: 624 LGLPDFIEVKGVTYCGESSASNLTMANVPWHEEVIRFVEEL-VDLLPDYEVACEHEHSNC 683

Query: 647 VLLAKTDKFKVDGQWFTWIDYEKFHDLVAS------GKPFDSKDYMAPTPSWAVYGSDEG 657
           +L+A   KFK++G+W TWIDYE+F +L+ +       + F + DY+A TP WA++GS E 
Sbjct: 684 MLIAH-KKFKINGEWCTWIDYERFQELIQAFGESKGSRTFTALDYVAKTPEWALFGSRER 726

[Close]

BLAST of Cla97C08G158680 vs. ExPASy Swiss-Prot
Match: Q08C92 (S-adenosyl-L-methionine-dependent tRNA 4-demethylwyosine synthase TYW1 OS=Danio rerio OX=7955 GN=tyw1 PE=2 SV=1)

HSP 1 Score: 597.8 bits (1540), Expect = 1.5e-169
Identity = 310/669 (46.34%), Postives = 417/669 (62.33%), Query Frame = 0


Query: 47  KLFFISQTGTSKALAHRLLNVLNSNGLVFDLVDPKDYEPEDLFKE-----ILVVFVASTW 106
           K+F+ SQTGT+K  A  L   + + G+  +++D KD++PED   E     I+ VF+ +T+
Sbjct: 80  KVFYGSQTGTAKGFAKELAEDVIAQGIQCEVIDMKDFDPEDRLAEECTSKIICVFLVATY 139

Query: 107 EDGGPPPHAKFLANWLSESAEDFRVGSLLLSQCKFAVFGVGSRAYGETFNAVAMDFSKRM 166
            DG P   A++   WL E++ DFR G   L   ++AVFG+G+  Y   FN V+    K +
Sbjct: 140 TDGQPTESAEWFCKWLEEASTDFRYGKTYLKGMRYAVFGLGNSVYVGHFNTVSKSIDKWL 199

Query: 167 KALGAKEILAVGEGDID-----GGDIDKYFDDWSRQLLKVVKVDAVGTGVDLDSGI---- 226
             L A  I+  GEGD +      G +   F  W  + L  ++  A G             
Sbjct: 200 WMLSAARIMTRGEGDCNVVKSRHGSVQADFQVWKGKFLNRLQALAKGEKKACSGNCKKAS 259

Query: 227 -------------------AGDSDAESVEEDEEEDTDLGEE----DIVDLEDIAG----- 286
                                +S+ E +E   +E++   +E     ++D+ED+       
Sbjct: 260 CKNKKKHKEEAEDNHSLAEKNNSEEELMESSSDEESSSEDEKSHGSVIDMEDLGNVMNHM 319

Query: 287 -KGPSR-----KSTNVVETNGKLNG-----KKVMVTPVIRASLEKQGYKIIGSHSGVKIC 346
            K   R     + +  V+ NG+        ++ M+TP +R SL KQGYK+IGSHSGVK+ 
Sbjct: 320 KKAKQRMEEDEEDSQRVKQNGERKSECEEERREMITPALRDSLTKQGYKLIGSHSGVKLR 379

Query: 347 RWTKSQLRGRGGCYKHSFYGIESHRCMEATPSLACANKCVFCWRHHTNPVGKSWQWEMDD 406
           RWTKS LRGRGGCYKH+FYGIESHRCME TPSLACANKCVFCWRHHTNPVG  W+W+MD 
Sbjct: 380 RWTKSMLRGRGGCYKHTFYGIESHRCMETTPSLACANKCVFCWRHHTNPVGTEWRWKMDP 439

Query: 407 PLDIVNSAIDLHTKMIKQMKGVPGVTQEKLDEGLSPRHCALSLVGEPIMYPEINTLVDEL 466
              I+  A++ H  MI+Q +GVPGV  E+ +EGL+ +HCALSLVGEPIMYPEIN+ +  L
Sbjct: 440 AEKIIQEAMENHRNMIRQFRGVPGVRPERFEEGLTVKHCALSLVGEPIMYPEINSFLKLL 499

Query: 467 HRRRISTFLVTNAQFPDKIKLLKPVTQLYVSVDAATKESLKAIDRPLFGDFWERFIDSLK 526
           H++ IS+FLVTNAQFP++I+ L PVTQLYVSVDA+TK+SLK IDRPLF DFW+RF+    
Sbjct: 500 HQQNISSFLVTNAQFPEEIRSLVPVTQLYVSVDASTKDSLKKIDRPLFKDFWQRFL---- 559

Query: 527 ALNDKQQRTVINDLCASIMLQDSLKALNDKQQRTVYRLTLVKGWNTEDIDAYSNLFSIGR 586
                                DSL+AL +KQQRTVYRLTLVK WN +++ AY++L ++G+
Sbjct: 560 ---------------------DSLRALGEKQQRTVYRLTLVKAWNVDELKAYADLIALGQ 619

Query: 587 PDFVEIKGVTYCGSSNTSKLTMENVPWHSDVKSFSEALAAKSRGEYEVACEHVHSCCVLL 646
           PDF+E+KGVTYCG S+ S LTM NVPWH +V  F + L A    +YE+ACEH HS C+LL
Sbjct: 620 PDFIEVKGVTYCGESSASSLTMANVPWHEEVIYFVQQL-ANLLPDYEIACEHEHSNCLLL 679

Query: 647 AKTDKFKVDGQWFTWIDYEKFHDLV------ASGKPFDSKDYMAPTPSWAVYGSDEGGFD 657
           A   KFKVDG+W+TWIDYE+F +L+         K F + DYMA TPSWAV+G+ E GFD
Sbjct: 680 A-NHKFKVDGEWWTWIDYERFQELIQQYEESGGTKNFSAMDYMAKTPSWAVFGAGERGFD 721

[Close]

BLAST of Cla97C08G158680 vs. ExPASy Swiss-Prot
Match: Q9NV66 (S-adenosyl-L-methionine-dependent tRNA 4-demethylwyosine synthase TYW1 OS=Homo sapiens OX=9606 GN=TYW1 PE=2 SV=2)

HSP 1 Score: 591.3 bits (1523), Expect = 1.4e-167
Identity = 312/671 (46.50%), Postives = 410/671 (61.10%), Query Frame = 0


Query: 47  KLFFISQTGTSKALAHRLLNVLNSNGLVFDLVDPKDYEPED-LFKEI----LVVFVASTW 106
           K+F+ SQTGT+K  A  L   + S  L   +++ K+Y+P+D L +E+    + VF+ +T+
Sbjct: 80  KIFYGSQTGTAKGFATVLAEAVTSLDLPVAIINLKEYDPDDHLIEEVTSKNVCVFLVATY 139

Query: 107 EDGGPPPHAKFLANWLSESAEDFRVGSLLLSQCKFAVFGVGSRAYGETFNAVAMDFSKRM 166
            DG P   A++   WL E++ DFR G   L   ++AVFG+G+ AY   FN V  +  K +
Sbjct: 140 TDGLPTESAEWFCKWLEEASIDFRFGKTYLKGMRYAVFGLGNSAYASHFNKVGKNVDKWL 199

Query: 167 KALGAKEILAVGEGDID-----GGDIDKYFDDWSRQLLKVVKVDAVGTGVDLDSGIAGDS 226
             LGA  +++ GEGD D      G I+  F  W  + +  ++    G       G     
Sbjct: 200 WMLGAHRVMSRGEGDCDVVKSKHGSIEADFRAWKTKFISQLQALQKGERKKSCGGHCKKG 259

Query: 227 DAESVEEDEEE--------------------------DTDLGEED------IVDLEDIAG 286
             ES +   EE                          + + G ED      IVD+ED+  
Sbjct: 260 KCESHQHGSEEREEGSHEQDELHHRDTEEEEPFESSSEEEFGGEDHQSLNSIVDVEDLGK 319

Query: 287 -----KGPSRKSTNVVETNGKLNG--------KKVMVTPVIRASLEKQGYKIIGSHSGVK 346
                K   R+     E +G            ++ M+TP +R +L KQGY++IGSHSGVK
Sbjct: 320 IMDHVKKEKREKEQQEEKSGLFRNMGRNEDGERRAMITPALREALTKQGYQLIGSHSGVK 379

Query: 347 ICRWTKSQLRGRGGCYKHSFYGIESHRCMEATPSLACANKCVFCWRHHTNPVGKSWQWEM 406
           +CRWTKS LRGRGGCYKH+FYGIESHRCME TPSLACANKCVFCWRHHTNPVG  W+W+M
Sbjct: 380 LCRWTKSMLRGRGGCYKHTFYGIESHRCMETTPSLACANKCVFCWRHHTNPVGTEWRWKM 439

Query: 407 DDPLDIVNSAIDLHTKMIKQMKGVPGVTQEKLDEGLSPRHCALSLVGEPIMYPEINTLVD 466
           D P  I+  AI+ H  MIKQ KGVPGV  E+ +EG++ +HCALSLVGEPIMYPEIN  + 
Sbjct: 440 DQPEMILKEAIENHQNMIKQFKGVPGVKAERFEEGMTVKHCALSLVGEPIMYPEINRFLK 499

Query: 467 ELHRRRISTFLVTNAQFPDKIKLLKPVTQLYVSVDAATKESLKAIDRPLFGDFWERFIDS 526
            LH+ +IS+FLVTNAQFP +I+ L+PVTQLYVSVDA+TK+SLK IDRPLF DFW+RF+  
Sbjct: 500 LLHQCKISSFLVTNAQFPAEIRNLEPVTQLYVSVDASTKDSLKKIDRPLFKDFWQRFL-- 559

Query: 527 LKALNDKQQRTVINDLCASIMLQDSLKALNDKQQRTVYRLTLVKGWNTEDIDAYSNLFSI 586
                                  DSLKAL  KQQRTVYRLTLVK WN +++ AY+ L S+
Sbjct: 560 -----------------------DSLKALAVKQQRTVYRLTLVKAWNVDELQAYAQLVSL 619

Query: 587 GRPDFVEIKGVTYCGSSNTSKLTMENVPWHSDVKSFSEALAAKSRGEYEVACEHVHSCCV 646
           G PDF+E+KGVTYCG S+ S LTM +VPWH +V  F   L      EYE+ACEH HS C+
Sbjct: 620 GNPDFIEVKGVTYCGESSASSLTMAHVPWHEEVVQFVHEL-VDLIPEYEIACEHEHSNCL 679

Query: 647 LLAKTDKFKVDGQWFTWIDYEKFHDLV------ASGKPFDSKDYMAPTPSWAVYGSDEGG 657
           L+A   KFK+ G+W+TWIDY +F +L+         K F +KDYMA TP WA++G+ E G
Sbjct: 680 LIAHR-KFKIGGEWWTWIDYNRFQELIQEYEDSGGSKTFSAKDYMARTPHWALFGASERG 723

[Close]

BLAST of Cla97C08G158680 vs. ExPASy TrEMBL
Match: A0A5A7VC83 (tRNA 4-demethylwyosine synthase (AdoMet-dependent) OS=Cucumis melo var. makuwa OX=1194695 GN=E6C27_scaffold82G005790 PE=3 SV=1)

HSP 1 Score: 1235.7 bits (3196), Expect = 0.0e+00
Identity = 611/666 (91.74%), Postives = 627/666 (94.14%), Query Frame = 0


Query: 1   MSFSSRSAKLTLLALLSASTLYCFYKSRRLRKLKLSLNPTPCSRRPKLFFISQTGTSKAL 60
           MSFSSRSAKLT+LALLSASTLYCFYKSRRLRKLKLSLNPT  SR+PKLFFISQTGTSKAL
Sbjct: 1   MSFSSRSAKLTILALLSASTLYCFYKSRRLRKLKLSLNPTLSSRKPKLFFISQTGTSKAL 60

Query: 61  AHRLLNVLNSNGLVFDLVDPKDYEPEDLFKEILVVFVASTWEDGGPPPHAKFLANWLSES 120
           AHRLLNVLNSNGLVFDLVDPKDYEPEDLFKE LV+FVASTWEDGGPPPHAKFLANWLSES
Sbjct: 61  AHRLLNVLNSNGLVFDLVDPKDYEPEDLFKETLVLFVASTWEDGGPPPHAKFLANWLSES 120

Query: 121 AEDFRVGSLLLSQCKFAVFGVGSRAYGETFNAVAMDFSKRMKALGAKEILAVGEGDIDGG 180
           AEDFRVGSLLLSQCKF+VFGVGSR YGET+NAVA DFSKRMKALGAKEIL +GEGD+DGG
Sbjct: 121 AEDFRVGSLLLSQCKFSVFGVGSRVYGETYNAVARDFSKRMKALGAKEILPIGEGDVDGG 180

Query: 181 DIDKYFDDWSRQLLKVVKVDAVGTGVDLDSGIAGDSDAESVEED-EEEDTDLGEEDIVDL 240
           DIDK FDDWSR+LLKVVKVDA G GV+L SGIAGDSDA SVEE+ EEED DLGEEDIVDL
Sbjct: 181 DIDKCFDDWSRKLLKVVKVDAEGNGVELCSGIAGDSDAVSVEEEYEEEDDDLGEEDIVDL 240

Query: 241 EDIAGKGPSRKSTNVVETNGKLNGKKVMVTPVIRASLEKQGYKIIGSHSGVKICRWTKSQ 300
           EDIAGKGPSRKSTNVVETNGKLNGKKVMVTPVIRASLEKQGYKIIGSHSGVKICRWTKSQ
Sbjct: 241 EDIAGKGPSRKSTNVVETNGKLNGKKVMVTPVIRASLEKQGYKIIGSHSGVKICRWTKSQ 300

Query: 301 LRGRGGCYKHSFYGIESHRCMEATPSLACANKCVFCWRHHTNPVGKSWQWEMDDPLDIVN 360
           LRGRGGCYKHSFYGIESHRCMEATPSLACANKCVFCWRHHTNPVGKSWQWEMDDPLDIVN
Sbjct: 301 LRGRGGCYKHSFYGIESHRCMEATPSLACANKCVFCWRHHTNPVGKSWQWEMDDPLDIVN 360

Query: 361 SAIDLHTKMIKQMKGVPGVTQEKLDEGLSPRHCALSLVGEPIMYPEINTLVDELHRRRIS 420
           SAIDLHTKMIKQMKGVPGVTQEKL+EGLSPRHCA SLVGEPIMYPEINTLVDELH+RRIS
Sbjct: 361 SAIDLHTKMIKQMKGVPGVTQEKLEEGLSPRHCAFSLVGEPIMYPEINTLVDELHQRRIS 420

Query: 421 TFLVTNAQFPDKIKLLKPVTQLYVSVDAATKESLKAIDRPLFGDFWERFIDSLKALNDKQ 480
           TFLVTNAQFPDKIKLLKPVTQLYVSVDAATKESLKAIDRPLFGDFWERFI          
Sbjct: 421 TFLVTNAQFPDKIKLLKPVTQLYVSVDAATKESLKAIDRPLFGDFWERFI---------- 480

Query: 481 QRTVINDLCASIMLQDSLKALNDKQQRTVYRLTLVKGWNTEDIDAYSNLFSIGRPDFVEI 540
                          DSLKALN+KQQRTVYRLTLVKGWNTEDIDAYSNLFSIGRPDFVEI
Sbjct: 481 ---------------DSLKALNEKQQRTVYRLTLVKGWNTEDIDAYSNLFSIGRPDFVEI 540

Query: 541 KGVTYCGSSNTSKLTMENVPWHSDVKSFSEALAAKSRGEYEVACEHVHSCCVLLAKTDKF 600
           KGVTYCGSSNTSKLTMENVPWHSDVKSFSEALAAKS+GEYEVACEHVHSCCVLLAKTDKF
Sbjct: 541 KGVTYCGSSNTSKLTMENVPWHSDVKSFSEALAAKSQGEYEVACEHVHSCCVLLAKTDKF 600

Query: 601 KVDGQWFTWIDYEKFHDLVASGKPFDSKDYMAPTPSWAVYGSDEGGFDPDQSRYRKERHH 660
           +VDGQWFTWIDYEKFHDLVASGKPFDSKDYMAPTPSWAVYGSDEGGFDPDQSRYRKERHH
Sbjct: 601 RVDGQWFTWIDYEKFHDLVASGKPFDSKDYMAPTPSWAVYGSDEGGFDPDQSRYRKERHH 641

Query: 661 RPKPTS 666
           +PKPTS
Sbjct: 661 KPKPTS 641

[Close]

BLAST of Cla97C08G158680 vs. ExPASy TrEMBL
Match: A0A1S3BAY2 (tRNA 4-demethylwyosine synthase (AdoMet-dependent) OS=Cucumis melo OX=3656 GN=LOC103487965 PE=3 SV=1)

HSP 1 Score: 1234.6 bits (3193), Expect = 0.0e+00
Identity = 610/666 (91.59%), Postives = 628/666 (94.29%), Query Frame = 0


Query: 1   MSFSSRSAKLTLLALLSASTLYCFYKSRRLRKLKLSLNPTPCSRRPKLFFISQTGTSKAL 60
           MSFSSRSAKLT+LALLSASTLYCFYKSRRLRKLKLSLNPT  SR+PKLFFISQTGTSKAL
Sbjct: 1   MSFSSRSAKLTILALLSASTLYCFYKSRRLRKLKLSLNPTLSSRKPKLFFISQTGTSKAL 60

Query: 61  AHRLLNVLNSNGLVFDLVDPKDYEPEDLFKEILVVFVASTWEDGGPPPHAKFLANWLSES 120
           AHRLLNVLNSNGLVFDLVDPKDYEPEDLFKE LV+FVASTWEDGGPPPHAKFLANWLSES
Sbjct: 61  AHRLLNVLNSNGLVFDLVDPKDYEPEDLFKETLVLFVASTWEDGGPPPHAKFLANWLSES 120

Query: 121 AEDFRVGSLLLSQCKFAVFGVGSRAYGETFNAVAMDFSKRMKALGAKEILAVGEGDIDGG 180
           AEDFRVGSLLLSQCKF+VFGVGSR YGET+NAVA DFSKRMK+LGAKEIL +GEGD+DGG
Sbjct: 121 AEDFRVGSLLLSQCKFSVFGVGSRVYGETYNAVARDFSKRMKSLGAKEILPIGEGDVDGG 180

Query: 181 DIDKYFDDWSRQLLKVVKVDAVGTGVDLDSGIAGDSDAESVEED-EEEDTDLGEEDIVDL 240
           DIDK FDDWSR+LLKVVKVDA G GV+L SGIAGDSDA SVEE+ EEED DLGEEDIVDL
Sbjct: 181 DIDKCFDDWSRKLLKVVKVDAEGNGVELCSGIAGDSDAVSVEEEYEEEDDDLGEEDIVDL 240

Query: 241 EDIAGKGPSRKSTNVVETNGKLNGKKVMVTPVIRASLEKQGYKIIGSHSGVKICRWTKSQ 300
           EDIAGKGPSRKSTNVVETNGKLNGKKVMVTPVIRASLEKQGYKIIGSHSGVKICRWTKSQ
Sbjct: 241 EDIAGKGPSRKSTNVVETNGKLNGKKVMVTPVIRASLEKQGYKIIGSHSGVKICRWTKSQ 300

Query: 301 LRGRGGCYKHSFYGIESHRCMEATPSLACANKCVFCWRHHTNPVGKSWQWEMDDPLDIVN 360
           LRGRGGCYKHSFYGIESHRCMEATPSLACANKCVFCWRHHTNPVGKSWQWEMDDPLDIVN
Sbjct: 301 LRGRGGCYKHSFYGIESHRCMEATPSLACANKCVFCWRHHTNPVGKSWQWEMDDPLDIVN 360

Query: 361 SAIDLHTKMIKQMKGVPGVTQEKLDEGLSPRHCALSLVGEPIMYPEINTLVDELHRRRIS 420
           SAIDLHTKMIKQMKGVPGVTQEKL+EGLSPRHCALSLVGEPIMYPEINTLVDELH+RRIS
Sbjct: 361 SAIDLHTKMIKQMKGVPGVTQEKLEEGLSPRHCALSLVGEPIMYPEINTLVDELHQRRIS 420

Query: 421 TFLVTNAQFPDKIKLLKPVTQLYVSVDAATKESLKAIDRPLFGDFWERFIDSLKALNDKQ 480
           TFLVTNAQFPDKIKLLKPVTQLYVSVDAATKESLKAIDRPLFGDFWERFI          
Sbjct: 421 TFLVTNAQFPDKIKLLKPVTQLYVSVDAATKESLKAIDRPLFGDFWERFI---------- 480

Query: 481 QRTVINDLCASIMLQDSLKALNDKQQRTVYRLTLVKGWNTEDIDAYSNLFSIGRPDFVEI 540
                          DSLKALN+KQQRTVYRLTLVKGWNTEDIDAYSNLFSIGRPDFVEI
Sbjct: 481 ---------------DSLKALNEKQQRTVYRLTLVKGWNTEDIDAYSNLFSIGRPDFVEI 540

Query: 541 KGVTYCGSSNTSKLTMENVPWHSDVKSFSEALAAKSRGEYEVACEHVHSCCVLLAKTDKF 600
           KGVTYCGSSNTSKLTMENVPWHSDVKSFSEALAAKS+GEYEVACEHVHSCCVLLAKTDKF
Sbjct: 541 KGVTYCGSSNTSKLTMENVPWHSDVKSFSEALAAKSQGEYEVACEHVHSCCVLLAKTDKF 600

Query: 601 KVDGQWFTWIDYEKFHDLVASGKPFDSKDYMAPTPSWAVYGSDEGGFDPDQSRYRKERHH 660
           +VDGQWFTWIDYEKFHDLVASGKPFDSKDY+APTPSWAVYGSDEGGFDPDQSRYRKERHH
Sbjct: 601 RVDGQWFTWIDYEKFHDLVASGKPFDSKDYIAPTPSWAVYGSDEGGFDPDQSRYRKERHH 641

Query: 661 RPKPTS 666
           +PKPTS
Sbjct: 661 KPKPTS 641

[Close]

BLAST of Cla97C08G158680 vs. ExPASy TrEMBL
Match: A0A0A0LPA4 (tRNA 4-demethylwyosine synthase (AdoMet-dependent) OS=Cucumis sativus OX=3659 GN=Csa_2G361620 PE=3 SV=1)

HSP 1 Score: 1233.4 bits (3190), Expect = 0.0e+00
Identity = 607/665 (91.28%), Postives = 625/665 (93.98%), Query Frame = 0


Query: 1   MSFSSRSAKLTLLALLSASTLYCFYKSRRLRKLKLSLNPTPCSRRPKLFFISQTGTSKAL 60
           MS SSRSAKLT+LALLSASTLYCFYKSRRLRKLKLSLNPT  SR+PKLFFISQTGTSKAL
Sbjct: 1   MSLSSRSAKLTILALLSASTLYCFYKSRRLRKLKLSLNPTLPSRKPKLFFISQTGTSKAL 60

Query: 61  AHRLLNVLNSNGLVFDLVDPKDYEPEDLFKEILVVFVASTWEDGGPPPHAKFLANWLSES 120
           AHRLLNVLNSNGLVFDLVDPKDYEPEDLFKE LV+FVASTWEDGGPPPHAKFLANWLSES
Sbjct: 61  AHRLLNVLNSNGLVFDLVDPKDYEPEDLFKETLVLFVASTWEDGGPPPHAKFLANWLSES 120

Query: 121 AEDFRVGSLLLSQCKFAVFGVGSRAYGETFNAVAMDFSKRMKALGAKEILAVGEGDIDGG 180
           AEDFRVGSLLLSQCKF+VFGVGSR YGETFNAVA DFSKR+KALGAKEIL +GEGD+DGG
Sbjct: 121 AEDFRVGSLLLSQCKFSVFGVGSRVYGETFNAVARDFSKRLKALGAKEILPIGEGDVDGG 180

Query: 181 DIDKYFDDWSRQLLKVVKVDAVGTGVDLDSGIAGDSDAESVEEDEEEDTDLGEEDIVDLE 240
           DIDK FDDWSR+LLKV+K DA G GV+L SGIAGDSDA SVEEDEEED DLGEEDIVDLE
Sbjct: 181 DIDKCFDDWSRKLLKVMKFDAEGNGVELCSGIAGDSDAVSVEEDEEEDDDLGEEDIVDLE 240

Query: 241 DIAGKGPSRKSTNVVETNGKLNGKKVMVTPVIRASLEKQGYKIIGSHSGVKICRWTKSQL 300
           DIAGKGPSRKSTN+VETNGKLNGKKVMVTPVIRASLEKQGYKIIGSHSGVKICRWTKSQL
Sbjct: 241 DIAGKGPSRKSTNIVETNGKLNGKKVMVTPVIRASLEKQGYKIIGSHSGVKICRWTKSQL 300

Query: 301 RGRGGCYKHSFYGIESHRCMEATPSLACANKCVFCWRHHTNPVGKSWQWEMDDPLDIVNS 360
           RGRGGCYKHSFYGIESHRCMEATPSLACANKCVFCWRHHTNPVGKSWQWEMDDPL+IVNS
Sbjct: 301 RGRGGCYKHSFYGIESHRCMEATPSLACANKCVFCWRHHTNPVGKSWQWEMDDPLEIVNS 360

Query: 361 AIDLHTKMIKQMKGVPGVTQEKLDEGLSPRHCALSLVGEPIMYPEINTLVDELHRRRIST 420
           AIDLHTKMIKQMKGVPGVTQEKL+EGLSPRHCALSLVGEPIMYPEINTLVDELHRRRIST
Sbjct: 361 AIDLHTKMIKQMKGVPGVTQEKLEEGLSPRHCALSLVGEPIMYPEINTLVDELHRRRIST 420

Query: 421 FLVTNAQFPDKIKLLKPVTQLYVSVDAATKESLKAIDRPLFGDFWERFIDSLKALNDKQQ 480
           FLVTNAQFPDKIKLLKPVTQLYVSVDAATKESLKAIDRPLFGDFWERFI           
Sbjct: 421 FLVTNAQFPDKIKLLKPVTQLYVSVDAATKESLKAIDRPLFGDFWERFI----------- 480

Query: 481 RTVINDLCASIMLQDSLKALNDKQQRTVYRLTLVKGWNTEDIDAYSNLFSIGRPDFVEIK 540
                         DSLKALN+KQQRTVYRLTLVKGWNTEDIDAYSNLFSIGRPDFVEIK
Sbjct: 481 --------------DSLKALNEKQQRTVYRLTLVKGWNTEDIDAYSNLFSIGRPDFVEIK 540

Query: 541 GVTYCGSSNTSKLTMENVPWHSDVKSFSEALAAKSRGEYEVACEHVHSCCVLLAKTDKFK 600
           GVTYCGSS TSKLTMENVPWHSDVKSFSEALAAKS+GEYEVACEHVHSCCVLLAKTDKF+
Sbjct: 541 GVTYCGSSTTSKLTMENVPWHSDVKSFSEALAAKSQGEYEVACEHVHSCCVLLAKTDKFR 600

Query: 601 VDGQWFTWIDYEKFHDLVASGKPFDSKDYMAPTPSWAVYGSDEGGFDPDQSRYRKERHHR 660
           VDGQWFTWIDY+KFHDLVASGKPFDSKDYMAPTPSWAVYGSDEGGFDPDQSRYRKERHH+
Sbjct: 601 VDGQWFTWIDYDKFHDLVASGKPFDSKDYMAPTPSWAVYGSDEGGFDPDQSRYRKERHHK 640

Query: 661 PKPTS 666
           PKPTS
Sbjct: 661 PKPTS 640

[Close]

BLAST of Cla97C08G158680 vs. ExPASy TrEMBL
Match: A0A6J1K9S7 (tRNA 4-demethylwyosine synthase (AdoMet-dependent) OS=Cucurbita maxima OX=3661 GN=LOC111492394 PE=3 SV=1)

HSP 1 Score: 1232.2 bits (3187), Expect = 0.0e+00
Identity = 611/665 (91.88%), Postives = 623/665 (93.68%), Query Frame = 0


Query: 1   MSFSSRSAKLTLLALLSASTLYCFYKSRRLRKLKLSLNPTPCSRRPKLFFISQTGTSKAL 60
           MSFSSRSAKL LLALLSASTLYCFYKSRRLRKLKLSLNPT CSRRPKLFFISQTGTSKAL
Sbjct: 1   MSFSSRSAKLALLALLSASTLYCFYKSRRLRKLKLSLNPTGCSRRPKLFFISQTGTSKAL 60

Query: 61  AHRLLNVLNSNGLVFDLVDPKDYEPEDLFKEILVVFVASTWEDGGPPPHAKFLANWLSES 120
           AHRLLNVLNSN L FD+VDPKD+EPEDLFKE LV+FVASTWEDGGPPPHAKFLANWLSES
Sbjct: 61  AHRLLNVLNSNELAFDIVDPKDFEPEDLFKEALVLFVASTWEDGGPPPHAKFLANWLSES 120

Query: 121 AEDFRVGSLLLSQCKFAVFGVGSRAYGETFNAVAMDFSKRMKALGAKEILAVGEGDIDGG 180
           AEDFRVGSLLLSQCKFAVFGVGSRAYGETFNAVA DFSKRMKALGAKEILAVGEGD+DGG
Sbjct: 121 AEDFRVGSLLLSQCKFAVFGVGSRAYGETFNAVARDFSKRMKALGAKEILAVGEGDVDGG 180

Query: 181 DIDKYFDDWSRQLLKVVKVDAVGTGVDLDSGIAGDSDAESVEEDEEEDTDLGEEDIVDLE 240
           DIDK FDDWSRQLLKV+KVDAVGTG DL SGI  D DAESVEED +ED D GEEDIVDLE
Sbjct: 181 DIDKCFDDWSRQLLKVLKVDAVGTGADLYSGIDEDFDAESVEED-DEDADPGEEDIVDLE 240

Query: 241 DIAGKGPSRKSTNVVETNGKLNGKKVMVTPVIRASLEKQGYKIIGSHSGVKICRWTKSQL 300
           DIAGKGPSRKSTNVVETNGKLNGKKVMVTPVIRASLEKQGYKIIGSHSGVKICRWTKSQL
Sbjct: 241 DIAGKGPSRKSTNVVETNGKLNGKKVMVTPVIRASLEKQGYKIIGSHSGVKICRWTKSQL 300

Query: 301 RGRGGCYKHSFYGIESHRCMEATPSLACANKCVFCWRHHTNPVGKSWQWEMDDPLDIVNS 360
           RGRGGCYKHSFYGIESHRCMEATPSLACANKCVFCWRHHTNPVGKSWQWEMDDPLDIVNS
Sbjct: 301 RGRGGCYKHSFYGIESHRCMEATPSLACANKCVFCWRHHTNPVGKSWQWEMDDPLDIVNS 360

Query: 361 AIDLHTKMIKQMKGVPGVTQEKLDEGLSPRHCALSLVGEPIMYPEINTLVDELHRRRIST 420
           AIDLHTKMIKQMKGVPGVTQEKL+EGLSPRHCALSLVGEPIMYPEINTLVDELHRRRIST
Sbjct: 361 AIDLHTKMIKQMKGVPGVTQEKLEEGLSPRHCALSLVGEPIMYPEINTLVDELHRRRIST 420

Query: 421 FLVTNAQFPDKIKLLKPVTQLYVSVDAATKESLKAIDRPLFGDFWERFIDSLKALNDKQQ 480
           FLVTNAQFPDKIK LKPVTQLYVSVDAATKESLKAIDRPLFGDFWERFI           
Sbjct: 421 FLVTNAQFPDKIKSLKPVTQLYVSVDAATKESLKAIDRPLFGDFWERFI----------- 480

Query: 481 RTVINDLCASIMLQDSLKALNDKQQRTVYRLTLVKGWNTEDIDAYSNLFSIGRPDFVEIK 540
                         DSLKALNDKQQRTVYRLTLVKGWNTEDIDAYSNLFS+G+PDFVEIK
Sbjct: 481 --------------DSLKALNDKQQRTVYRLTLVKGWNTEDIDAYSNLFSLGKPDFVEIK 540

Query: 541 GVTYCGSSNTSKLTMENVPWHSDVKSFSEALAAKSRGEYEVACEHVHSCCVLLAKTDKFK 600
           GVTYCGSSNTSKLTMENVPWHSDVKSFSEALAAKS+GEYEVACEHVHSCCVLLAKTDKF+
Sbjct: 541 GVTYCGSSNTSKLTMENVPWHSDVKSFSEALAAKSQGEYEVACEHVHSCCVLLAKTDKFR 600

Query: 601 VDGQWFTWIDYEKFHDLVASGKPFDSKDYMAPTPSWAVYGSDEGGFDPDQSRYRKERHHR 660
           VDGQWFTWIDYEKFHDLVASGKPFDSKDYMAPTPSWAVYG+DEGGFDP QSRYRKERHHR
Sbjct: 601 VDGQWFTWIDYEKFHDLVASGKPFDSKDYMAPTPSWAVYGADEGGFDPVQSRYRKERHHR 639

Query: 661 PKPTS 666
           PKPTS
Sbjct: 661 PKPTS 639

[Close]

BLAST of Cla97C08G158680 vs. ExPASy TrEMBL
Match: A0A6J1HDZ5 (tRNA 4-demethylwyosine synthase (AdoMet-dependent) OS=Cucurbita moschata OX=3662 GN=LOC111462691 PE=3 SV=1)

HSP 1 Score: 1221.1 bits (3158), Expect = 0.0e+00
Identity = 605/665 (90.98%), Postives = 620/665 (93.23%), Query Frame = 0


Query: 1   MSFSSRSAKLTLLALLSASTLYCFYKSRRLRKLKLSLNPTPCSRRPKLFFISQTGTSKAL 60
           MS SSRSAKL LLALLSASTLYCFYKSRRLRKLKLSLNPT CSRRPKLFFISQTGTSKAL
Sbjct: 1   MSLSSRSAKLALLALLSASTLYCFYKSRRLRKLKLSLNPTGCSRRPKLFFISQTGTSKAL 60

Query: 61  AHRLLNVLNSNGLVFDLVDPKDYEPEDLFKEILVVFVASTWEDGGPPPHAKFLANWLSES 120
           AHRLLNVLNSN L FD+VDPKD+EPEDLFKE LV+FVASTWEDGGPPPHAKFLA+WLSES
Sbjct: 61  AHRLLNVLNSNELAFDIVDPKDFEPEDLFKETLVLFVASTWEDGGPPPHAKFLASWLSES 120

Query: 121 AEDFRVGSLLLSQCKFAVFGVGSRAYGETFNAVAMDFSKRMKALGAKEILAVGEGDIDGG 180
           AEDFRVGSLLLSQCKFAVFGVGSRAYGETFNAVA DFSKRMKALGAKEILAVGEGD+DGG
Sbjct: 121 AEDFRVGSLLLSQCKFAVFGVGSRAYGETFNAVARDFSKRMKALGAKEILAVGEGDVDGG 180

Query: 181 DIDKYFDDWSRQLLKVVKVDAVGTGVDLDSGIAGDSDAESVEEDEEEDTDLGEEDIVDLE 240
           DIDK FDDWSRQLLKV+KVDAVGTG DL SGI  D DAESVEED +ED D GEEDIVDLE
Sbjct: 181 DIDKCFDDWSRQLLKVLKVDAVGTGADLYSGIDEDFDAESVEED-DEDADPGEEDIVDLE 240

Query: 241 DIAGKGPSRKSTNVVETNGKLNGKKVMVTPVIRASLEKQGYKIIGSHSGVKICRWTKSQL 300
           DIAGKGPSRKSTNVVETNGKLNGKKVMVTPVIRASLEKQGYKIIGSHSGVKICRWTKSQL
Sbjct: 241 DIAGKGPSRKSTNVVETNGKLNGKKVMVTPVIRASLEKQGYKIIGSHSGVKICRWTKSQL 300

Query: 301 RGRGGCYKHSFYGIESHRCMEATPSLACANKCVFCWRHHTNPVGKSWQWEMDDPLDIVNS 360
           RGRGGCYKHSFYGIESHRCMEATPSLACANKCVFCWRHHTNPVGKSWQWEMDDPLDIVNS
Sbjct: 301 RGRGGCYKHSFYGIESHRCMEATPSLACANKCVFCWRHHTNPVGKSWQWEMDDPLDIVNS 360

Query: 361 AIDLHTKMIKQMKGVPGVTQEKLDEGLSPRHCALSLVGEPIMYPEINTLVDELHRRRIST 420
           AIDLH KMIKQMKGVPGVTQEKL+EGLSPRHCALSLVGEPIMYPEINTLVDELHRRRIST
Sbjct: 361 AIDLHKKMIKQMKGVPGVTQEKLEEGLSPRHCALSLVGEPIMYPEINTLVDELHRRRIST 420

Query: 421 FLVTNAQFPDKIKLLKPVTQLYVSVDAATKESLKAIDRPLFGDFWERFIDSLKALNDKQQ 480
           FLVTNAQFPDKIK LKPVTQLYVSVDAATK+SLKAIDRPLFGDFWERFI           
Sbjct: 421 FLVTNAQFPDKIKSLKPVTQLYVSVDAATKDSLKAIDRPLFGDFWERFI----------- 480

Query: 481 RTVINDLCASIMLQDSLKALNDKQQRTVYRLTLVKGWNTEDIDAYSNLFSIGRPDFVEIK 540
                         DSLKALNDKQQRTVYRLTLVKGWNTEDIDAYSNLFS+G+PDFVEIK
Sbjct: 481 --------------DSLKALNDKQQRTVYRLTLVKGWNTEDIDAYSNLFSLGKPDFVEIK 540

Query: 541 GVTYCGSSNTSKLTMENVPWHSDVKSFSEALAAKSRGEYEVACEHVHSCCVLLAKTDKFK 600
           GVTYCGSSNTSKLTMENVPWHSDVKSFSEALAAKS+GEYEVACEHVHSCCVLLAKTDKF+
Sbjct: 541 GVTYCGSSNTSKLTMENVPWHSDVKSFSEALAAKSQGEYEVACEHVHSCCVLLAKTDKFR 600

Query: 601 VDGQWFTWIDYEKFHDLVASGKPFDSKDYMAPTPSWAVYGSDEGGFDPDQSRYRKERHHR 660
           +DGQWFTWIDYEKFH LVASGKPFDSKDYMAPTPSWAVYG+DEGGFDP QSRYRKERHHR
Sbjct: 601 IDGQWFTWIDYEKFHALVASGKPFDSKDYMAPTPSWAVYGADEGGFDPVQSRYRKERHHR 639

Query: 661 PKPTS 666
           PKPTS
Sbjct: 661 PKPTS 639

[Close]

BLAST of Cla97C08G158680 vs. TAIR 10
Match: AT1G75200.1 (flavodoxin family protein / radical SAM domain-containing protein )

HSP 1 Score: 937.6 bits (2422), Expect = 5.6e-273
Identity = 473/669 (70.70%), Postives = 534/669 (79.82%), Query Frame = 0


Query: 4   SSRSAKLTLLALLSASTLYCFYKSRRLRKLK-LSLNP--TPCSRRPKLFFISQTGTSKAL 63
           SS   +L  +ALLSA+T YC +K RRL+ LK LSLNP  T  + R K+FFISQTGT+KAL
Sbjct: 5   SSVRVRLAFVALLSATTFYCIHKYRRLKHLKNLSLNPSSTLKASRGKIFFISQTGTAKAL 64

Query: 64  AHRLLNVLNSNGLVFDLVDPKDYEPEDLFKEILVVFVASTWEDGGPPPHAKFLANWLSES 123
           A RL  +  SN + FD+VDP  YEPEDL KE LV+F+ASTW+ G PP + +FL NWL ES
Sbjct: 65  AQRLHELCASNDIAFDIVDPHSYEPEDLPKETLVLFIASTWDGGKPPKNGEFLVNWLGES 124

Query: 124 AEDFRVGSLLLSQCKFAVFGVGSRAYGETFNAVAMDFSKRMKALGAKEILAVGEGDIDGG 183
           AEDFRVGSLLLS CKFAVFGVGSRAYGE++NAVA + S RM  LG  E++ VGEGD+D G
Sbjct: 125 AEDFRVGSLLLSDCKFAVFGVGSRAYGESYNAVAKELSSRMIGLGGLEMIPVGEGDVDDG 184

Query: 184 DIDKYFDDWSRQLLKVVKVDAV--GTGVDLDSGIAGDSDAE----SVEEDEEEDTDLGEE 243
           ++D+ F DW   +++V+K  +     GV    G A + D E    + EEDE+ D D G  
Sbjct: 185 ELDRAFQDWCDGVIRVLKGGSAQETNGVSQQIG-AVEDDLEYYDSTDEEDEDNDADGG-- 244

Query: 244 DIVDLEDIAGKGPSRKSTNVVETNGKLNGKKVMVTPVIRASLEKQGYKIIGSHSGVKICR 303
            IVDLEDIAGK PS+++  V  T  K++GKK MVTPVIRASL KQGYKIIGSHSGVKICR
Sbjct: 245 -IVDLEDIAGKAPSKRNGVVKVT--KVDGKKEMVTPVIRASLTKQGYKIIGSHSGVKICR 304

Query: 304 WTKSQLRGRGGCYKHSFYGIESHRCMEATPSLACANKCVFCWRHHTNPVGKSWQWEMDDP 363
           WTKSQLRGRGGCYKHSFYGIESHRCME TPSLACANKCVFCWRHHTNPVGKSWQW+MD+P
Sbjct: 305 WTKSQLRGRGGCYKHSFYGIESHRCMETTPSLACANKCVFCWRHHTNPVGKSWQWKMDEP 364

Query: 364 LDIVNSAIDLHTKMIKQMKGVPGVTQEKLDEGLSPRHCALSLVGEPIMYPEINTLVDELH 423
             IV  A+DLH  MIKQMKGVPGVT EKL EGL+PRHCALSLVGEPIMYPEIN LVDELH
Sbjct: 365 SVIVKGALDLHKNMIKQMKGVPGVTPEKLQEGLNPRHCALSLVGEPIMYPEINALVDELH 424

Query: 424 RRRISTFLVTNAQFPDKIKLLKPVTQLYVSVDAATKESLKAIDRPLFGDFWERFIDSLKA 483
            RRISTFLVTNAQFP+KI ++KP+TQLYVSVDAATKESLKAIDRPLF DFWERFI     
Sbjct: 425 GRRISTFLVTNAQFPEKILMMKPITQLYVSVDAATKESLKAIDRPLFADFWERFI----- 484

Query: 484 LNDKQQRTVINDLCASIMLQDSLKALNDKQQRTVYRLTLVKGWNTEDIDAYSNLFSIGRP 543
                               DSLKAL +KQQRTVYRLTLVKGWNTE++DAY NLFSIG+P
Sbjct: 485 --------------------DSLKALQEKQQRTVYRLTLVKGWNTEELDAYFNLFSIGKP 544

Query: 544 DFVEIKGVTYCGSSNTSKLTMENVPWHSDVKSFSEALAAKSRGEYEVACEHVHSCCVLLA 603
           DF+EIKGVTYCGSS TSKLTMENVPWH+DVK+FSEAL+ KS GEYEVACEH HSCCVLL 
Sbjct: 545 DFIEIKGVTYCGSSATSKLTMENVPWHTDVKAFSEALSLKSNGEYEVACEHAHSCCVLLG 604

Query: 604 KTDKFKVDGQWFTWIDYEKFHDLVASGKPFDSKDYMAPTPSWAVYGSDEGGFDPDQSRYR 663
           +T+KFKVDG+WFTWIDYEKFHDLVASG+PF S DYMA TPSWAVYG+ EGGFDP Q RY+
Sbjct: 605 RTEKFKVDGKWFTWIDYEKFHDLVASGEPFTSTDYMAQTPSWAVYGAQEGGFDPGQLRYK 642

The following BLAST results are available for this feature:

BLAST of Cla97C08G158680 vs. NCBI nr


Match Name	E-value	Identity	Description
XP_038884961.1	0.0e+00	93.23	S-adenosyl-L-methionine-dependent tRNA 4-demethylwyosine synthase [Benincasa his...	[more]
KAA0065238.1	0.0e+00	91.74	S-adenosyl-L-methionine-dependent tRNA 4-demethylwyosine synthase [Cucumis melo ...	[more]
XP_008444711.1	0.0e+00	91.59	PREDICTED: S-adenosyl-L-methionine-dependent tRNA 4-demethylwyosine synthase [Cu...	[more]
XP_004152698.1	0.0e+00	91.28	S-adenosyl-L-methionine-dependent tRNA 4-demethylwyosine synthase [Cucumis sativ...	[more]
XP_022997485.1	0.0e+00	91.88	S-adenosyl-L-methionine-dependent tRNA 4-demethylwyosine synthase [Cucurbita max...	[more]

BLAST of Cla97C08G158680 vs. ExPASy Swiss-Prot


Match Name	E-value	Identity	Description
Q8RXN5	7.9e-272	70.70	S-adenosyl-L-methionine-dependent tRNA 4-demethylwyosine synthase OS=Arabidopsis...	[more]
Q8H8N3	8.2e-253	66.27	S-adenosyl-L-methionine-dependent tRNA 4-demethylwyosine synthase OS=Oryza sativ...	[more]
Q2KHP8	2.1e-171	47.62	S-adenosyl-L-methionine-dependent tRNA 4-demethylwyosine synthase TYW1 OS=Xenopu...	[more]
Q08C92	1.5e-169	46.34	S-adenosyl-L-methionine-dependent tRNA 4-demethylwyosine synthase TYW1 OS=Danio ...	[more]
Q9NV66	1.4e-167	46.50	S-adenosyl-L-methionine-dependent tRNA 4-demethylwyosine synthase TYW1 OS=Homo s...	[more]

BLAST of Cla97C08G158680 vs. ExPASy TrEMBL


Match Name	E-value	Identity	Description
A0A5A7VC83	0.0e+00	91.74	tRNA 4-demethylwyosine synthase (AdoMet-dependent) OS=Cucumis melo var. makuwa O...	[more]
A0A1S3BAY2	0.0e+00	91.59	tRNA 4-demethylwyosine synthase (AdoMet-dependent) OS=Cucumis melo OX=3656 GN=LO...	[more]
A0A0A0LPA4	0.0e+00	91.28	tRNA 4-demethylwyosine synthase (AdoMet-dependent) OS=Cucumis sativus OX=3659 GN...	[more]
A0A6J1K9S7	0.0e+00	91.88	tRNA 4-demethylwyosine synthase (AdoMet-dependent) OS=Cucurbita maxima OX=3661 G...	[more]
A0A6J1HDZ5	0.0e+00	90.98	tRNA 4-demethylwyosine synthase (AdoMet-dependent) OS=Cucurbita moschata OX=3662...	[more]

BLAST of Cla97C08G158680 vs. TAIR 10


Match Name	E-value	Identity	Description
AT1G75200.1	5.6e-273	70.70	flavodoxin family protein / radical SAM domain-containing protein	[more]

InterPro

Analysis Name: InterPro Annotations of Watermelon (97103) v2.5
Date Performed: 2022-01-31


IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
IPR001094	Flavodoxin-like	PRINTS	PR00369	FLAVODOXIN	coord: 95..106 score: 38.41 coord: 47..60 score: 35.6 coord: 133..143 score: 47.16 coord: 158..177 score: 33.2
IPR013917	tRNA wybutosine-synthesis	PFAM	PF08608	Wyosine_form	coord: 532..595 e-value: 4.0E-26 score: 91.1
IPR013785	Aldolase-type TIM barrel	GENE3D	3.20.20.70	Aldolase class I	coord: 491..607 e-value: 3.3E-36 score: 127.0 coord: 251..488 e-value: 4.8E-114 score: 382.9
IPR029039	Flavoprotein-like superfamily	GENE3D	3.40.50.360		coord: 26..203 e-value: 7.2E-38 score: 131.8
IPR029039	Flavoprotein-like superfamily	SUPERFAMILY	52218	Flavoproteins	coord: 47..197
IPR008254	Flavodoxin/nitric oxide synthase	PFAM	PF00258	Flavodoxin_1	coord: 48..189 e-value: 8.5E-24 score: 84.4
IPR008254	Flavodoxin/nitric oxide synthase	PROSITE	PS50902	FLAVODOXIN_LIKE	coord: 46..194 score: 22.081478
IPR007197	Radical SAM	PFAM	PF04055	Radical_SAM	coord: 324..484 e-value: 3.4E-20 score: 73.1
IPR007197	Radical SAM	SFLD	SFLDS00029	Radical_SAM	coord: 324..484 e-value: 8.6E-24 score: 79.3
IPR007197	Radical SAM	PROSITE	PS51918	RADICAL_SAM	coord: 312..580 score: 12.005415
None	No IPR available	MOBIDB_LITE	mobidb-lite	disorder_prediction	coord: 210..231
None	No IPR available	MOBIDB_LITE	mobidb-lite	disorder_prediction	coord: 627..665
None	No IPR available	MOBIDB_LITE	mobidb-lite	disorder_prediction	coord: 216..231
None	No IPR available	PANTHER	PTHR13930:SF0	S-ADENOSYL-L-METHIONINE-DEPENDENT TRNA 4-DEMETHYLWYOSINE SYNTHASE	coord: 3..484
None	No IPR available	PANTHER	PTHR13930:SF0	S-ADENOSYL-L-METHIONINE-DEPENDENT TRNA 4-DEMETHYLWYOSINE SYNTHASE	coord: 493..659
None	No IPR available	CDD	cd01335	Radical_SAM	coord: 324..475 e-value: 3.21304E-6 score: 46.5578
None	No IPR available	SUPERFAMILY	102114	Radical SAM enzymes	coord: 325..485
IPR034556	S-adenosyl-L-methionine-dependent tRNA 4-demethylwyosine synthase	PANTHER	PTHR13930	S-ADENOSYL-L-METHIONINE-DEPENDENT TRNA 4-DEMETHYLWYOSINE SYNTHASE	coord: 3..484
IPR034556	S-adenosyl-L-methionine-dependent tRNA 4-demethylwyosine synthase	PANTHER	PTHR13930	S-ADENOSYL-L-METHIONINE-DEPENDENT TRNA 4-DEMETHYLWYOSINE SYNTHASE	coord: 493..659

Relationships

The following mRNA feature(s) are a part of this gene:


Feature Name	Unique Name	Type
Cla97C08G158680.2	Cla97C08G158680.2	mRNA

GO Annotation

GO Assignments

This gene is annotated with the following GO terms.

Category	Term Accession	Term Name
biological_process	GO:0032259	methylation
biological_process	GO:0031591	wybutosine biosynthetic process
biological_process	GO:0008033	tRNA processing
cellular_component	GO:0016021	integral component of membrane
molecular_function	GO:0051539	4 iron, 4 sulfur cluster binding
molecular_function	GO:0010181	FMN binding
molecular_function	GO:0046872	metal ion binding
molecular_function	GO:0008168	methyltransferase activity
molecular_function	GO:0102521	tRNA-4-demethylwyosine synthase activity
molecular_function	GO:0003824	catalytic activity
molecular_function	GO:0051536	iron-sulfur cluster binding