Cla97C08G151790 (gene) Watermelon (97103) v2.5

Overview
NameCla97C08G151790
Typegene
OrganismCitrullus lanatus subsp. vulgaris cv. 97103 (Watermelon (97103) v2.5)
Descriptionprotein NRT1/ PTR FAMILY 4.6-like
LocationCla97Chr08: 20133657 .. 20135122 (+)
RNA-Seq ExpressionCla97C08G151790
SyntenyCla97C08G151790
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: exonCDSpolypeptide
Hold the cursor over a type above to highlight its positions in the sequence below.
ATGTTGACAGTGCAAGCGAAGGTGAGTTCATCATGCGATGAGGGGAACTCGAAGGGATGCAAGGAAGTAGGGGGGAGAGAGGCGGCGGTGCTGTTCATGGGGCTGTATCTGGTGGCGTTGGGGGTTGGCGGCATAAAGGGGTCGTTACCGGCACACGGGGCGGAGCAGTTCGACGAAGGCAGCCCACAAGGGAGGAAGAGGAGGTCAAGTTTCTTCAACTACTTTGTCTTCAGTCTCTCATGTGGAGCTCTTATTGCAGTGACTTTCGTGGTTTGGGTTGAAGACAATTTGGGGTGGCAATGGGGATTTGGAATCTCAACTATCAGCATCTTTCTATCTATTCCTTTGTTCCTTGCTGGTTCCCCTTTTTATCGTAACAAAATCCCCACTGGTAGCCCTCTTACCACCATTCTCAAGGTATGTTTCTATCAATGGCAATGGTGTTTTATCCATTTTTTTAAAATATATATTAAAAGTATTTGATTTTGAATGATGAATGATGGGTTTAGGTATTAGTAGCAGCGACATTGAACCGTGAGAGCAGCAAGAAAACATCAACAAACGCAGTGGCAAGCCTAGCAAGAAGCCCATCCACCTCAACCCCAAAGCTGATTAACAAGGAGCTAACACAAAACAATACAACTTGTCCAACCCAAAGCCTCAAATTCCTAAACAAAGCAGTACAAAACCCAGCCTTCCACCCATCACTAAAATGCACAACCCAACAACTAGAAGACGTAAAAATAGTCTTAAAAGTCCTCCCCATCTTCGCTTGCACCATCATCCTCAACTCATGCTTAGCTCAACTCTCCACTTTCTCCATCGAACAAGCCTCTACAATGAACACCAAAATCGCCTCTCTCAAACTCCCACCAGCCTCTCTCCCAATCTTCCCTGTCCTCTTCATCATCGTCCTTGCCCCGCTCTACGACCACCTCATCCTCCCCTTTGCTCGAACCCTTACTCGCACCGAGGCCGGCATCACCCACCTCCAACGCATCGGCATCGGCCTCCTTCTCTCCATCCTCGCCATGGCCATTGCTGCCCTCGTCGAGACCAAGCGTAAGGGCGTGGCTGATGCTCACCCAGATGCCCACCCGCTGCCCATTACCTTCCTTTGGATTGCCTTTCAGTACTTGTTTTTGGGGTCTGCGGATTTGTTCACATTGGCGGGTTTGTTGGAGTTTTTCTTCACGGAGGCTCCTGCTACAATGCGGTCGTTGGCTACTGCCTTATCTTGGGCCTCCTTGGCTGTTGGGTACTATCTAAGTTCCGTCATTGTATCGGTTGTGAACCGTGTGACTGCCAAAGCTTCCCACACGCCTTGGCTGTCTGGCCACAACATCAATCATTATCGTCTTTCTAACTTCTATTGGCTCATGTGTGCGCTTAGTGCCTTCAATTTCTTGCACTACCTTTTCTGGGCACTTAAGTATAAATATCGATCAACTCAAGGGAACAAATGA

mRNA sequence

ATGTTGACAGTGCAAGCGAAGGTGAGTTCATCATGCGATGAGGGGAACTCGAAGGGATGCAAGGAAGTAGGGGGGAGAGAGGCGGCGGTGCTGTTCATGGGGCTGTATCTGGTGGCGTTGGGGGTTGGCGGCATAAAGGGGTCGTTACCGGCACACGGGGCGGAGCAGTTCGACGAAGGCAGCCCACAAGGGAGGAAGAGGAGGTCAAGTTTCTTCAACTACTTTGTCTTCAGTCTCTCATGTGGAGCTCTTATTGCAGTGACTTTCGTGGTTTGGGTTGAAGACAATTTGGGGTGGCAATGGGGATTTGGAATCTCAACTATCAGCATCTTTCTATCTATTCCTTTGTTCCTTGCTGGTTCCCCTTTTTATCGTAACAAAATCCCCACTGGTAGCCCTCTTACCACCATTCTCAAGGTATTAGTAGCAGCGACATTGAACCGTGAGAGCAGCAAGAAAACATCAACAAACGCAGTGGCAAGCCTAGCAAGAAGCCCATCCACCTCAACCCCAAAGCTGATTAACAAGGAGCTAACACAAAACAATACAACTTGTCCAACCCAAAGCCTCAAATTCCTAAACAAAGCAGTACAAAACCCAGCCTTCCACCCATCACTAAAATGCACAACCCAACAACTAGAAGACGTAAAAATAGTCTTAAAAGTCCTCCCCATCTTCGCTTGCACCATCATCCTCAACTCATGCTTAGCTCAACTCTCCACTTTCTCCATCGAACAAGCCTCTACAATGAACACCAAAATCGCCTCTCTCAAACTCCCACCAGCCTCTCTCCCAATCTTCCCTGTCCTCTTCATCATCGTCCTTGCCCCGCTCTACGACCACCTCATCCTCCCCTTTGCTCGAACCCTTACTCGCACCGAGGCCGGCATCACCCACCTCCAACGCATCGGCATCGGCCTCCTTCTCTCCATCCTCGCCATGGCCATTGCTGCCCTCGTCGAGACCAAGCGTAAGGGCGTGGCTGATGCTCACCCAGATGCCCACCCGCTGCCCATTACCTTCCTTTGGATTGCCTTTCAGTACTTGTTTTTGGGGTCTGCGGATTTGTTCACATTGGCGGGTTTGTTGGAGTTTTTCTTCACGGAGGCTCCTGCTACAATGCGGTCGTTGGCTACTGCCTTATCTTGGGCCTCCTTGGCTGTTGGGTACTATCTAAGTTCCGTCATTGTATCGGTTGTGAACCGTGTGACTGCCAAAGCTTCCCACACGCCTTGGCTGTCTGGCCACAACATCAATCATTATCGTCTTTCTAACTTCTATTGGCTCATGTGTGCGCTTAGTGCCTTCAATTTCTTGCACTACCTTTTCTGGGCACTTAAGTATAAATATCGATCAACTCAAGGGAACAAATGA

Coding sequence (CDS)

ATGTTGACAGTGCAAGCGAAGGTGAGTTCATCATGCGATGAGGGGAACTCGAAGGGATGCAAGGAAGTAGGGGGGAGAGAGGCGGCGGTGCTGTTCATGGGGCTGTATCTGGTGGCGTTGGGGGTTGGCGGCATAAAGGGGTCGTTACCGGCACACGGGGCGGAGCAGTTCGACGAAGGCAGCCCACAAGGGAGGAAGAGGAGGTCAAGTTTCTTCAACTACTTTGTCTTCAGTCTCTCATGTGGAGCTCTTATTGCAGTGACTTTCGTGGTTTGGGTTGAAGACAATTTGGGGTGGCAATGGGGATTTGGAATCTCAACTATCAGCATCTTTCTATCTATTCCTTTGTTCCTTGCTGGTTCCCCTTTTTATCGTAACAAAATCCCCACTGGTAGCCCTCTTACCACCATTCTCAAGGTATTAGTAGCAGCGACATTGAACCGTGAGAGCAGCAAGAAAACATCAACAAACGCAGTGGCAAGCCTAGCAAGAAGCCCATCCACCTCAACCCCAAAGCTGATTAACAAGGAGCTAACACAAAACAATACAACTTGTCCAACCCAAAGCCTCAAATTCCTAAACAAAGCAGTACAAAACCCAGCCTTCCACCCATCACTAAAATGCACAACCCAACAACTAGAAGACGTAAAAATAGTCTTAAAAGTCCTCCCCATCTTCGCTTGCACCATCATCCTCAACTCATGCTTAGCTCAACTCTCCACTTTCTCCATCGAACAAGCCTCTACAATGAACACCAAAATCGCCTCTCTCAAACTCCCACCAGCCTCTCTCCCAATCTTCCCTGTCCTCTTCATCATCGTCCTTGCCCCGCTCTACGACCACCTCATCCTCCCCTTTGCTCGAACCCTTACTCGCACCGAGGCCGGCATCACCCACCTCCAACGCATCGGCATCGGCCTCCTTCTCTCCATCCTCGCCATGGCCATTGCTGCCCTCGTCGAGACCAAGCGTAAGGGCGTGGCTGATGCTCACCCAGATGCCCACCCGCTGCCCATTACCTTCCTTTGGATTGCCTTTCAGTACTTGTTTTTGGGGTCTGCGGATTTGTTCACATTGGCGGGTTTGTTGGAGTTTTTCTTCACGGAGGCTCCTGCTACAATGCGGTCGTTGGCTACTGCCTTATCTTGGGCCTCCTTGGCTGTTGGGTACTATCTAAGTTCCGTCATTGTATCGGTTGTGAACCGTGTGACTGCCAAAGCTTCCCACACGCCTTGGCTGTCTGGCCACAACATCAATCATTATCGTCTTTCTAACTTCTATTGGCTCATGTGTGCGCTTAGTGCCTTCAATTTCTTGCACTACCTTTTCTGGGCACTTAAGTATAAATATCGATCAACTCAAGGGAACAAATGA

Protein sequence

MLTVQAKVSSSCDEGNSKGCKEVGGREAAVLFMGLYLVALGVGGIKGSLPAHGAEQFDEGSPQGRKRRSSFFNYFVFSLSCGALIAVTFVVWVEDNLGWQWGFGISTISIFLSIPLFLAGSPFYRNKIPTGSPLTTILKVLVAATLNRESSKKTSTNAVASLARSPSTSTPKLINKELTQNNTTCPTQSLKFLNKAVQNPAFHPSLKCTTQQLEDVKIVLKVLPIFACTIILNSCLAQLSTFSIEQASTMNTKIASLKLPPASLPIFPVLFIIVLAPLYDHLILPFARTLTRTEAGITHLQRIGIGLLLSILAMAIAALVETKRKGVADAHPDAHPLPITFLWIAFQYLFLGSADLFTLAGLLEFFFTEAPATMRSLATALSWASLAVGYYLSSVIVSVVNRVTAKASHTPWLSGHNINHYRLSNFYWLMCALSAFNFLHYLFWALKYKYRSTQGNK
Homology
BLAST of Cla97C08G151790 vs. NCBI nr
Match: XP_038886228.1 (protein NRT1/ PTR FAMILY 4.6-like [Benincasa hispida])

HSP 1 Score: 840.9 bits (2171), Expect = 5.3e-240
Identity = 437/458 (95.41%), Postives = 447/458 (97.60%), Query Frame = 0

Query: 1   MLTVQAKVSSSCDEGNSKGCKEVGGREAAVLFMGLYLVALGVGGIKGSLPAHGAEQFDEG 60
           MLTVQAKVSSSC+EGNSKGCKEVGGREAAVLF+GLYLVALGVGGIKGSLPAHGAEQFDEG
Sbjct: 116 MLTVQAKVSSSCEEGNSKGCKEVGGREAAVLFIGLYLVALGVGGIKGSLPAHGAEQFDEG 175

Query: 61  SPQGRKRRSSFFNYFVFSLSCGALIAVTFVVWVEDNLGWQWGFGISTISIFLSIPLFLAG 120
           SPQGRKRRSSFFNYFVFSLSCGALIAVTFVVWVEDNLGWQWGFGISTISIFLSIPLFLAG
Sbjct: 176 SPQGRKRRSSFFNYFVFSLSCGALIAVTFVVWVEDNLGWQWGFGISTISIFLSIPLFLAG 235

Query: 121 SPFYRNKIPTGSPLTTILKVLVAATLNRESSKKTSTNAVASLARSPSTSTPK-LINKELT 180
           SPFYRNKIPTGSPLTTILKVLVAA LNR SS K +TNAVASLARSPSTST K LINKELT
Sbjct: 236 SPFYRNKIPTGSPLTTILKVLVAAALNRGSSNKPATNAVASLARSPSTSTAKQLINKELT 295

Query: 181 QNNTTCPTQSLKFLNKAVQNPAFHPSLKCTTQQLEDVKIVLKVLPIFACTIILNSCLAQL 240
           QNNTT PTQSLKFLNKAVQNP FHPSLKCTTQQLEDVKIVLKVLPIFACTIILNSCLAQL
Sbjct: 296 QNNTTNPTQSLKFLNKAVQNPPFHPSLKCTTQQLEDVKIVLKVLPIFACTIILNSCLAQL 355

Query: 241 STFSIEQASTMNTKIASLKLPPASLPIFPVLFIIVLAPLYDHLILPFARTLTRTEAGITH 300
           STFSIEQASTMNTKI+SLK+PPASLPIFPVLFII+LAPLYDHLI+PFARTLTRTEAGITH
Sbjct: 356 STFSIEQASTMNTKISSLKVPPASLPIFPVLFIILLAPLYDHLIIPFARTLTRTEAGITH 415

Query: 301 LQRIGIGLLLSILAMAIAALVETKRKGVADAHPDAHPLPITFLWIAFQYLFLGSADLFTL 360
           LQRIG+GLLLS+LAMA+AALVETKRK VA AHPDAHPLPITFLWIAFQYLFLGSADLFTL
Sbjct: 416 LQRIGVGLLLSVLAMAVAALVETKRKAVAGAHPDAHPLPITFLWIAFQYLFLGSADLFTL 475

Query: 361 AGLLEFFFTEAPATMRSLATALSWASLAVGYYLSSVIVSVVNRVTAKASHTPWLSGHNIN 420
           AGLLEFFFTEAPATMRSLATALSWASLAVGYYLSSVIVSVVNRVTAKASHTPWLSGHNIN
Sbjct: 476 AGLLEFFFTEAPATMRSLATALSWASLAVGYYLSSVIVSVVNRVTAKASHTPWLSGHNIN 535

Query: 421 HYRLSNFYWLMCALSAFNFLHYLFWALKYKYRSTQGNK 458
           HYRLSNFYWLMCALS FNFLHYLFWALKYKYRSTQGNK
Sbjct: 536 HYRLSNFYWLMCALSGFNFLHYLFWALKYKYRSTQGNK 573

BLAST of Cla97C08G151790 vs. NCBI nr
Match: XP_011657481.1 (protein NRT1/ PTR FAMILY 4.6 [Cucumis sativus] >APY23675.1 NRT1.2-like nitrate transporter [Cucumis sativus] >KAE8647291.1 hypothetical protein Csa_004297 [Cucumis sativus])

HSP 1 Score: 803.9 bits (2075), Expect = 7.2e-229
Identity = 416/460 (90.43%), Postives = 439/460 (95.43%), Query Frame = 0

Query: 1   MLTVQAKVSSSCDEGNSKGCKEVGGREAAVLFMGLYLVALGVGGIKGSLPAHGAEQFDEG 60
           MLTVQAKVSSSC+EG+SKGCKEVGG+E AVLF+GLYLVALGVGGIKGSLPAHGAEQFDE 
Sbjct: 116 MLTVQAKVSSSCEEGSSKGCKEVGGKEMAVLFIGLYLVALGVGGIKGSLPAHGAEQFDES 175

Query: 61  SPQGRKRRSSFFNYFVFSLSCGALIAVTFVVWVEDNLGWQWGFGISTISIFLSIPLFLAG 120
           +PQGRKRRSSFFNYFVFSLSCGALIAVTFVVW+EDNLGWQWGFGISTISIFLSIPLF AG
Sbjct: 176 TPQGRKRRSSFFNYFVFSLSCGALIAVTFVVWIEDNLGWQWGFGISTISIFLSIPLFFAG 235

Query: 121 SPFYRNKIPTGSPLTTILKVLVAATLNRESSKKTSTNAVASLARSPS-TSTPK-LINKEL 180
           SPFYRNKIPTGSPLTTILKVLVAATLNR S+KK +TNAVASLARSPS TSTPK LINK+L
Sbjct: 236 SPFYRNKIPTGSPLTTILKVLVAATLNRRSNKKPATNAVASLARSPSTTSTPKQLINKDL 295

Query: 181 TQNNTTCPTQSLKFLNKAVQNPAFHPSLKCTTQQLEDVKIVLKVLPIFACTIILNSCLAQ 240
           T+NN T PTQSLKFLNKAVQNP FHPSLKCTTQQLEDVKIV+KVLPIFACTIILNSCLAQ
Sbjct: 296 TENNVTTPTQSLKFLNKAVQNPPFHPSLKCTTQQLEDVKIVIKVLPIFACTIILNSCLAQ 355

Query: 241 LSTFSIEQASTMNTKIASLKLPPASLPIFPVLFIIVLAPLYDHLILPFARTLTRTEAGIT 300
           LSTFSIEQA+TMNTKI SLK+PPASLP+FPVLFII+LAPLYDHLI+PFAR+LTRTEAGIT
Sbjct: 356 LSTFSIEQAATMNTKIYSLKIPPASLPVFPVLFIIILAPLYDHLIIPFARSLTRTEAGIT 415

Query: 301 HLQRIGIGLLLSILAMAIAALVETKRKGVADAHPDAH-PLPITFLWIAFQYLFLGSADLF 360
           HLQRIG+GLLLS+LAMA+AALVETKRK VADA+PD H PLPITFLWIAFQYLFLGSADLF
Sbjct: 416 HLQRIGVGLLLSVLAMAVAALVETKRKAVADANPDVHGPLPITFLWIAFQYLFLGSADLF 475

Query: 361 TLAGLLEFFFTEAPATMRSLATALSWASLAVGYYLSSVIVSVVNRVTAKASHTPWLSGHN 420
           TLAGLLEFFFTEAP TMRSLATALSWASLAVGYYLSSVIVSVVN V+AKA H PWLSGHN
Sbjct: 476 TLAGLLEFFFTEAPTTMRSLATALSWASLAVGYYLSSVIVSVVNHVSAKAWHRPWLSGHN 535

Query: 421 INHYRLSNFYWLMCALSAFNFLHYLFWALKYKYRSTQGNK 458
           INHYRLSNFYW+MC LS  NFLHYLFWALKYKYRSTQGNK
Sbjct: 536 INHYRLSNFYWVMCVLSGLNFLHYLFWALKYKYRSTQGNK 575

BLAST of Cla97C08G151790 vs. NCBI nr
Match: XP_008456458.1 (PREDICTED: protein NRT1/ PTR FAMILY 4.6-like [Cucumis melo] >KAA0036440.1 protein NRT1/ PTR FAMILY 4.6-like [Cucumis melo var. makuwa] >TYK00047.1 protein NRT1/ PTR FAMILY 4.6-like [Cucumis melo var. makuwa])

HSP 1 Score: 792.0 bits (2044), Expect = 2.8e-225
Identity = 413/460 (89.78%), Postives = 438/460 (95.22%), Query Frame = 0

Query: 1   MLTVQAKVSSSCDEGNSKGCKEVGGREAAVLFMGLYLVALGVGGIKGSLPAHGAEQFDEG 60
           MLTVQAKVSSSC+ G +KGCKEVGG+E AVLF+GLYLVALGVGGIKGSLPAHGAEQFDE 
Sbjct: 116 MLTVQAKVSSSCEAG-TKGCKEVGGKEVAVLFIGLYLVALGVGGIKGSLPAHGAEQFDES 175

Query: 61  SPQGRKRRSSFFNYFVFSLSCGALIAVTFVVWVEDNLGWQWGFGISTISIFLSIPLFLAG 120
           + QGRKRRSSFFNYFVFSLSCGAL+AVTFVVWVEDNLGWQWGFGISTIS FLSIPLF AG
Sbjct: 176 TTQGRKRRSSFFNYFVFSLSCGALVAVTFVVWVEDNLGWQWGFGISTISTFLSIPLFFAG 235

Query: 121 SPFYRNKIPTGSPLTTILKVLVAATLNRESSKKTSTNAVASLARSPS-TSTPK-LINKEL 180
           SPFYRNKIPTGSPLTTILKVLVAATLNR S+KK +TNAVASLARSPS TSTPK LINK+L
Sbjct: 236 SPFYRNKIPTGSPLTTILKVLVAATLNRRSNKKPATNAVASLARSPSTTSTPKPLINKDL 295

Query: 181 TQNNTTCPTQSLKFLNKAVQNPAFHPSLKCTTQQLEDVKIVLKVLPIFACTIILNSCLAQ 240
           T++NTT PTQSLKFLNKAVQNP FHPS+KCTTQQLEDVKIV+KVLPIFACTIILNSCLAQ
Sbjct: 296 TESNTTTPTQSLKFLNKAVQNPPFHPSVKCTTQQLEDVKIVIKVLPIFACTIILNSCLAQ 355

Query: 241 LSTFSIEQASTMNTKIASLKLPPASLPIFPVLFIIVLAPLYDHLILPFARTLTRTEAGIT 300
           LSTFSIEQA+TMNTKI+SLK+PPASLP+FPVLFIIVLAPLYDHLI+PFAR+LTRTEAGIT
Sbjct: 356 LSTFSIEQAATMNTKISSLKIPPASLPVFPVLFIIVLAPLYDHLIIPFARSLTRTEAGIT 415

Query: 301 HLQRIGIGLLLSILAMAIAALVETKRKGVADAHPDAH-PLPITFLWIAFQYLFLGSADLF 360
           HLQRIG+GLLLS+LAMA+AALVETKRK VADA+PD   PLPITFLWIAFQYLFLGSADLF
Sbjct: 416 HLQRIGVGLLLSVLAMAVAALVETKRKAVADANPDVQGPLPITFLWIAFQYLFLGSADLF 475

Query: 361 TLAGLLEFFFTEAPATMRSLATALSWASLAVGYYLSSVIVSVVNRVTAKASHTPWLSGHN 420
           TLAGLLEFFFTEAPATMRSLATALSWASLAVGYYLSSVIVSVVN V+AKA H PWLSGHN
Sbjct: 476 TLAGLLEFFFTEAPATMRSLATALSWASLAVGYYLSSVIVSVVNHVSAKAWHRPWLSGHN 535

Query: 421 INHYRLSNFYWLMCALSAFNFLHYLFWALKYKYRSTQGNK 458
           INHYRLSNFYW+MC LS FNFLHYLFWALKYKYRSTQGNK
Sbjct: 536 INHYRLSNFYWVMCVLSGFNFLHYLFWALKYKYRSTQGNK 574

BLAST of Cla97C08G151790 vs. NCBI nr
Match: XP_022946066.1 (protein NRT1/ PTR FAMILY 4.6-like [Cucurbita moschata])

HSP 1 Score: 688.7 bits (1776), Expect = 3.4e-194
Identity = 357/457 (78.12%), Postives = 404/457 (88.40%), Query Frame = 0

Query: 1   MLTVQAKVSSSCDEGNSKGCKEVGGREAAVLFMGLYLVALGVGGIKGSLPAHGAEQFDEG 60
           +LTV+AK +++                AAV+F+GLYLVA+GVGGIKGSLP HGAEQF+EG
Sbjct: 116 ILTVEAKAATAA--------------SAAVVFVGLYLVAVGVGGIKGSLPTHGAEQFEEG 175

Query: 61  SPQGRKRRSSFFNYFVFSLSCGALIAVTFVVWVEDNLGWQWGFGISTISIFLSIPLFLAG 120
           + +GR+ RSSFFNYFVFSLSCGA++AVT VVW+EDNLGW+WGFGISTI+I +SIPLFLAG
Sbjct: 176 TAEGRRGRSSFFNYFVFSLSCGAVVAVTLVVWMEDNLGWEWGFGISTIAILVSIPLFLAG 235

Query: 121 SPFYRNKIPTGSPLTTILKVLVAATLNRESSKKTSTNAVASLARSPSTSTPKLINKELTQ 180
           S FYRNKIP G+PLTTILKVLVAATLN  S  KT++NAVAS+ RSPST+TPK   KE T 
Sbjct: 236 SCFYRNKIPAGAPLTTILKVLVAATLNGRSRNKTASNAVASMTRSPSTTTPK-PTKEPT- 295

Query: 181 NNTTCPTQSLKFLNKAVQNPAFHPSLKCTTQQLEDVKIVLKVLPIFACTIILNSCLAQLS 240
           + T  PTQ+LKFLNKA+Q P FHPSL CTTQQLEDVK+VLK+LPIFACTIILNSCLAQLS
Sbjct: 296 HTTPPPTQTLKFLNKAIQTPPFHPSLSCTTQQLEDVKVVLKLLPIFACTIILNSCLAQLS 355

Query: 241 TFSIEQASTMNTKIASLKLPPASLPIFPVLFIIVLAPLYDHLILPFARTLTRTEAGITHL 300
           TFS+EQASTMNTKI S+KLPPASLPIFP+LFII+LAPLY+HLI+PFARTLTRTE+GITHL
Sbjct: 356 TFSVEQASTMNTKIGSIKLPPASLPIFPILFIILLAPLYNHLIIPFARTLTRTESGITHL 415

Query: 301 QRIGIGLLLSILAMAIAALVETKRKGVADAHPDAHPLPITFLWIAFQYLFLGSADLFTLA 360
           QRIG+GLLLSI+AMAIAAL+E KRK +ADAHP AHPLPITFLWI FQYLFLGSADLFTLA
Sbjct: 416 QRIGVGLLLSIVAMAIAALIEVKRKAIADAHPAAHPLPITFLWIGFQYLFLGSADLFTLA 475

Query: 361 GLLEFFFTEAPATMRSLATALSWASLAVGYYLSSVIVSVVNRVTAKASHTPWLSGHNINH 420
           GLLEFFFTEAP+TMRSLATALSWASLAVGYYLSSVIVS+VNRVTAK+SH+PWLS  +INH
Sbjct: 476 GLLEFFFTEAPSTMRSLATALSWASLAVGYYLSSVIVSLVNRVTAKSSHSPWLSAQDINH 535

Query: 421 YRLSNFYWLMCALSAFNFLHYLFWALKYKYRSTQGNK 458
           Y L  FYWLMCALSA NFLH+LFWA+KYKYRST  +K
Sbjct: 536 YDLHKFYWLMCALSALNFLHFLFWAIKYKYRSTLASK 556

BLAST of Cla97C08G151790 vs. NCBI nr
Match: XP_022999670.1 (protein NRT1/ PTR FAMILY 4.6-like [Cucurbita maxima])

HSP 1 Score: 685.3 bits (1767), Expect = 3.7e-193
Identity = 358/453 (79.03%), Postives = 399/453 (88.08%), Query Frame = 0

Query: 1   MLTVQAKVSSSCDEGNSKGCKEVGGREAAVLFMGLYLVALGVGGIKGSLPAHGAEQFDEG 60
           +LTV+AK +S+                AA +F+GLYLVA+GVGGIKGSL AHGAEQF+EG
Sbjct: 117 ILTVEAKAASAA--------------SAAAVFVGLYLVAVGVGGIKGSLAAHGAEQFEEG 176

Query: 61  SPQGRKRRSSFFNYFVFSLSCGALIAVTFVVWVEDNLGWQWGFGISTISIFLSIPLFLAG 120
           + +GR+ RSSFFNYFVFSLSCGAL+ VT VVW+EDNLGW+WGFGISTI+I +SIPLFL+G
Sbjct: 177 TAEGRRGRSSFFNYFVFSLSCGALVGVTLVVWIEDNLGWEWGFGISTIAILVSIPLFLSG 236

Query: 121 SPFYRNKIPTGSPLTTILKVLVAATLNRESSKKTSTNAVASLARSPSTSTPKLINKELTQ 180
           S FYRNKIPT +PLTTILKVLVAATLN     KT+TNAVAS+ RSPST+TPK   KE T 
Sbjct: 237 SCFYRNKIPTAAPLTTILKVLVAATLNGRRRNKTATNAVASMTRSPSTTTPK-PTKEPT- 296

Query: 181 NNTTCPTQSLKFLNKAVQNPAFHPSLKCTTQQLEDVKIVLKVLPIFACTIILNSCLAQLS 240
            +TT PTQ+LKFLNKA+Q P FHPSL CTTQQLEDVKIVLK+LPIFACTIILNSCLAQLS
Sbjct: 297 -HTTPPTQTLKFLNKAIQTPPFHPSLSCTTQQLEDVKIVLKLLPIFACTIILNSCLAQLS 356

Query: 241 TFSIEQASTMNTKIASLKLPPASLPIFPVLFIIVLAPLYDHLILPFARTLTRTEAGITHL 300
           TFS+EQASTMNT+I S+K PPASLPIFPV+FII+LAPLY+HLI+PFARTLTRTE+GITHL
Sbjct: 357 TFSVEQASTMNTQIGSIKFPPASLPIFPVIFIIILAPLYNHLIVPFARTLTRTESGITHL 416

Query: 301 QRIGIGLLLSILAMAIAALVETKRKGVADAHPDAHPLPITFLWIAFQYLFLGSADLFTLA 360
           QRIG+GLLLSI+AM IAAL+E KRK VADAHP AHPLPITFLWIAFQYLFLGSADLFTLA
Sbjct: 417 QRIGVGLLLSIVAMVIAALIEVKRKAVADAHPAAHPLPITFLWIAFQYLFLGSADLFTLA 476

Query: 361 GLLEFFFTEAPATMRSLATALSWASLAVGYYLSSVIVSVVNRVTAKASHTPWLSGHNINH 420
           GLLEFFFTEAP TMRSLATALSWASLAVGYYLSSVIVS+VNRVTAK+SH+PWLSG +INH
Sbjct: 477 GLLEFFFTEAPPTMRSLATALSWASLAVGYYLSSVIVSLVNRVTAKSSHSPWLSGQDINH 536

Query: 421 YRLSNFYWLMCALSAFNFLHYLFWALKYKYRST 454
           Y L  FYWLMCALSA NFLH+LFWALKYKYRST
Sbjct: 537 YDLHKFYWLMCALSALNFLHFLFWALKYKYRST 552

BLAST of Cla97C08G151790 vs. ExPASy Swiss-Prot
Match: Q8H157 (Protein NRT1/ PTR FAMILY 4.6 OS=Arabidopsis thaliana OX=3702 GN=NPF4.6 PE=1 SV=1)

HSP 1 Score: 589.0 bits (1517), Expect = 4.7e-167
Identity = 316/473 (66.81%), Postives = 380/473 (80.34%), Query Frame = 0

Query: 1   MLTVQAKVSS----SCDEGNSKGCKEVGGREAAVLFMGLYLVALGVGGIKGSLPAHGAEQ 60
           +LT+QA+  S    SCD   S  C+EV G +AA+LF+GLYLVALGVGGIKGSL +HGAEQ
Sbjct: 113 ILTIQARTPSLMPPSCD---SPTCEEVSGSKAAMLFVGLYLVALGVGGIKGSLASHGAEQ 172

Query: 61  FDEGSPQGRKRRSSFFNYFVFSLSCGALIAVTFVVWVEDNLGWQWGFGISTISIFLSIPL 120
           FDE +P+GRK+RS+FFNYFVF L+CGAL+AVTFVVW+EDN GW+WGFG+STI+IF+SI +
Sbjct: 173 FDESTPKGRKQRSTFFNYFVFCLACGALVAVTFVVWLEDNKGWEWGFGVSTIAIFVSILI 232

Query: 121 FLAGSPFYRNKIPTGSPLTTILKVLVAATLNRESSKKTSTNAVASLARSPSTSTPKLINK 180
           FL+GS FYRNKIP GSPLTTILKVL+AA++ +  S  +S+NAVAS++ SPS        K
Sbjct: 233 FLSGSRFYRNKIPCGSPLTTILKVLLAASV-KCCSSGSSSNAVASMSVSPSNHCVSKGKK 292

Query: 181 EL-TQNNTTCP------------TQSLKFLNKAVQNPAFHPSLKCTTQQLEDVKIVLKVL 240
           E+ +Q     P            T SLK LN A      H  L+CT QQ+EDVKIVLK+L
Sbjct: 293 EVESQGELEKPRQEEALPPRAQLTNSLKVLNGAADEKPVHRLLECTVQQVEDVKIVLKML 352

Query: 241 PIFACTIILNSCLAQLSTFSIEQASTMNTKIASLKLPPASLPIFPVLFIIVLAPLYDHLI 300
           PIFACTI+LN CLAQLSTFS++QA++MNTKI SLK+PPASLPIFPV+FI++LAP+YDHLI
Sbjct: 353 PIFACTIMLNCCLAQLSTFSVQQAASMNTKIGSLKIPPASLPIFPVVFIMILAPIYDHLI 412

Query: 301 LPFARTLTRTEAGITHLQRIGIGLLLSILAMAIAALVETKRKGVADAH---PDAHPLPIT 360
           +PFAR  T+TE G+THLQRIG+GL+LSILAMA+AALVE KRKGVA           LP+T
Sbjct: 413 IPFARKATKTETGVTHLQRIGVGLVLSILAMAVAALVEIKRKGVAKDSGLLDSKETLPVT 472

Query: 361 FLWIAFQYLFLGSADLFTLAGLLEFFFTEAPATMRSLATALSWASLAVGYYLSSVIVSVV 420
           FLWIA QYLFLGSADLFTLAGLLE+FFTEAP++MRSLAT+LSWASLA+GYYLSSVIVS+V
Sbjct: 473 FLWIALQYLFLGSADLFTLAGLLEYFFTEAPSSMRSLATSLSWASLAMGYYLSSVIVSIV 532

Query: 421 NRVTAKASHTPWLSGHNINHYRLSNFYWLMCALSAFNFLHYLFWALKYKYRST 454
           N +T  + +TPWL G +IN Y+L  FYWLMC LSA NFLHYLFWA++YKYRST
Sbjct: 533 NSITGSSGNTPWLRGKSINRYKLDYFYWLMCVLSAANFLHYLFWAMRYKYRST 581

BLAST of Cla97C08G151790 vs. ExPASy Swiss-Prot
Match: Q8VYE4 (Protein NRT1/ PTR FAMILY 4.5 OS=Arabidopsis thaliana OX=3702 GN=NPF4.5 PE=2 SV=1)

HSP 1 Score: 552.4 bits (1422), Expect = 4.9e-156
Identity = 295/460 (64.13%), Postives = 360/460 (78.26%), Query Frame = 0

Query: 1   MLTVQAKVSS----SCDEGNSKGCKEVGGREAAVLFMGLYLVALGVGGIKGSLPAHGAEQ 60
           +LT+QA+  S     C    +  C+ VGG +AA LF+GLYLV+LG+GGIKGSLP+HGAEQ
Sbjct: 115 LLTIQARRPSLMPPPCKSSAALRCEVVGGSKAAFLFVGLYLVSLGIGGIKGSLPSHGAEQ 174

Query: 61  FDEGSPQGRKRRSSFFNYFVFSLSCGALIAVTFVVWVEDNLGWQWGFGISTISIFLSIPL 120
           FDEG+P+GRK+RS+FFNY+VF LSCGAL+AVTFVVW+EDN GW+WGFG+STISIFLSI +
Sbjct: 175 FDEGTPKGRKQRSTFFNYYVFCLSCGALVAVTFVVWIEDNKGWEWGFGVSTISIFLSILV 234

Query: 121 FLAGSPFYRNKIPTGSPLTTILKVLVAATLNRESSKKTSTNAVASLARSPSTSTPKLINK 180
           FL GS FY+NKIP GSPLTTI KVL+AA++   SSK +S +  +   +S         ++
Sbjct: 235 FLLGSRFYKNKIPRGSPLTTIFKVLLAASIVSCSSKTSSNHFTSREVQSE--------HE 294

Query: 181 ELTQNNTTCPTQSLKFLNKAVQNPAFHPSLKCTTQQLEDVKIVLKVLPIFACTIILNSCL 240
           E T + +   T SL  LNKA++    H  L+CT QQ+EDVKIVLK+LPIF CTI+LN CL
Sbjct: 295 EKTPSQSL--TNSLTCLNKAIEGKTHHIWLECTVQQVEDVKIVLKMLPIFGCTIMLNCCL 354

Query: 241 AQLSTFSIEQASTMNTKIASLKLPPASLPIFPVLFIIVLAPLYDHLILPFARTLTRTEAG 300
           AQLST+S+ QA+TMN KI +  +P ASLP+FPV+F+++LAP YDHLI+PFAR +T++E G
Sbjct: 355 AQLSTYSVHQAATMNRKIVNFNVPSASLPVFPVVFMLILAPTYDHLIIPFARKVTKSEIG 414

Query: 301 ITHLQRIGIGLLLSILAMAIAALVETKRKGV---ADAHPDAHPLPITFLWIAFQYLFLGS 360
           ITHLQRIG+GL+LSI+AMA+AALVE KRK V   A        LPITFLWIA QYLFLGS
Sbjct: 415 ITHLQRIGVGLVLSIVAMAVAALVELKRKQVAREAGLLDSEETLPITFLWIALQYLFLGS 474

Query: 361 ADLFTLAGLLEFFFTEAPATMRSLATALSWASLAVGYYLSSVIVSVVNRVTAKASHTPWL 420
           ADLFTLAGLLEFFFTEAP++MRSLAT+LSWASLA+GYYLSSV+V +VNRVT  A  +PWL
Sbjct: 475 ADLFTLAGLLEFFFTEAPSSMRSLATSLSWASLALGYYLSSVMVPIVNRVTKSAGQSPWL 534

Query: 421 SGHNINHYRLSNFYWLMCALSAFNFLHYLFWALKYKYRST 454
            G  +N  RL  FYWLMC LS  NFLHYLFWA +YKY ST
Sbjct: 535 -GEKLNRNRLDLFYWLMCVLSVVNFLHYLFWAKRYKYIST 563

BLAST of Cla97C08G151790 vs. ExPASy Swiss-Prot
Match: Q9FM20 (Protein NRT1/ PTR FAMILY 4.7 OS=Arabidopsis thaliana OX=3702 GN=NPF4.7 PE=2 SV=2)

HSP 1 Score: 507.3 bits (1305), Expect = 1.8e-142
Identity = 268/435 (61.61%), Postives = 328/435 (75.40%), Query Frame = 0

Query: 31  LFMGLYLVALGVGGIKGSLPAHGAEQFDEGSPQGRKRRSSFFNYFVFSLSCGALIAVTFV 90
           LF+GLYLVALGVGGIKGSLP HGAEQFDE +  GR++RS FFNYF+FSLSCGALIAVT V
Sbjct: 162 LFVGLYLVALGVGGIKGSLPPHGAEQFDEETSSGRRQRSFFFNYFIFSLSCGALIAVTVV 221

Query: 91  VWVEDNLGWQWGFGISTISIFLSIPLFLAGSPFYRNKIPTGSPLTTILKVLVAATLNRES 150
           VW+EDN GW +GFG+ST +I +S+P+FLAGS  YR K+P+GSP+TT+ KVL AA   +  
Sbjct: 222 VWLEDNKGWSYGFGVSTAAILISVPVFLAGSRVYRLKVPSGSPITTLFKVLTAALYAKYK 281

Query: 151 SKKTSTNAVASLARSPSTSTPKLINKELTQNNTTCPTQSL-KFLNKAVQNPAFHP-SLKC 210
            ++TS   V    R+         +  +T+ N       L  FL + V+     P  L+C
Sbjct: 282 KRRTSRIVVTCHTRND-------CDDSVTKQNCDGDDGFLGSFLGEVVRERESLPRPLRC 341

Query: 211 TTQQLEDVKIVLKVLPIFACTIILNSCLAQLSTFSIEQASTMNTKIASLKLPPASLPIFP 270
           T +Q++DVKIV+K+LPIF  TI+LN CLAQLSTFS++QASTMNTK+ S  +PPA+LP+FP
Sbjct: 342 TEEQVKDVKIVIKILPIFMSTIMLNCCLAQLSTFSVQQASTMNTKLGSFTVPPAALPVFP 401

Query: 271 VLFIIVLAPLYDHLILPFARTLTRTEAGITHLQRIGIGLLLSILAMAIAALVETKRKGVA 330
           V+F+++LAP Y+HL+LP AR  T+TE GITHLQRIG GL+LSI+AMA+AALVETKRK V 
Sbjct: 402 VVFMMILAPTYNHLLLPLARKSTKTETGITHLQRIGTGLVLSIVAMAVAALVETKRKHVV 461

Query: 331 --------DAHPDAHPLPITFLWIAFQYLFLGSADLFTLAGLLEFFFTEAPATMRSLATA 390
                    +   + PLPITFLW+A QY+FLGSADLFTLAG++EFFFTEAP+TMRSLAT+
Sbjct: 462 VSCCSNNNSSSYSSSPLPITFLWVAIQYVFLGSADLFTLAGMMEFFFTEAPSTMRSLATS 521

Query: 391 LSWASLAVGYYLSSVIVSVVNRVTAKASHTPWLSGHNINHYRLSNFYWLMCALSAFNFLH 450
           LSWASLA+GYY SSV+VS VN VT    H PWL G N+N Y L  FYWLMC LS  NFLH
Sbjct: 522 LSWASLAMGYYFSSVLVSAVNFVTGLNHHNPWLLGENLNQYHLERFYWLMCVLSGINFLH 581

Query: 451 YLFWALKYKYRSTQG 456
           YLFWA +Y YRS QG
Sbjct: 582 YLFWASRYVYRSNQG 589

BLAST of Cla97C08G151790 vs. ExPASy Swiss-Prot
Match: Q93VV5 (Protein NRT1/ PTR FAMILY 4.3 OS=Arabidopsis thaliana OX=3702 GN=NPF4.3 PE=2 SV=1)

HSP 1 Score: 294.3 bits (752), Expect = 2.4e-78
Identity = 186/475 (39.16%), Postives = 280/475 (58.95%), Query Frame = 0

Query: 1   MLTVQAKVSS----SCDEGNSKGCKEVGGREAAVLFMGLYLVALGVGGIKGSLPAHGAEQ 60
           +L+VQA +       C+    + C+E  G +A + FM LYLVALG G +K ++ AHGA+Q
Sbjct: 129 LLSVQAHLPQLKPPKCNPLIDQTCEEAKGFKAMIFFMALYLVALGSGCVKPNMIAHGADQ 188

Query: 61  FDEGSPQGRKRRSSFFNYFVFSLSCGALIAVTFVVWVEDNLGWQWGFGISTISIFLSIPL 120
           F +  P+  KR SS+FN   F+ S G LIA+T +VWV+ + G   GFG+S  ++ + I  
Sbjct: 189 FSQSHPKQSKRLSSYFNAAYFAFSMGELIALTLLVWVQTHSGMDIGFGVSAAAMTMGIIS 248

Query: 121 FLAGSPFYRNKIPTGSPLTTILKVLVAATLNRESSKKTSTNAVASLARSPSTSTPKLINK 180
            ++G+ ++RNK P  S  T I  V+VAA L R+ +               S S P++++ 
Sbjct: 249 LVSGTMYFRNKRPRRSIFTPIAHVIVAAILKRKLA---------------SPSDPRMLHG 308

Query: 181 ELTQNNTTCPTQSL------KFLNKAV-----QNPAFHPSLKCTTQQLEDVKIVLKVLPI 240
           +    N   P+ +L      +FL+KA       N    P   CT  Q+E VK ++ ++PI
Sbjct: 309 DHHVANDVVPSSTLPHTPRFRFLDKACIKIQDTNTKESPWRLCTVTQVEQVKTLISLVPI 368

Query: 241 FACTIILNSCLAQLSTFSIEQASTMNTKIA-SLKLPPASLPIFPVLFIIVLAPLYDHLIL 300
           FA TI+ N+ LAQL TFS++Q S+MNT+++ S  +PPASL   P + +I L PLYD  ++
Sbjct: 369 FASTIVFNTILAQLQTFSVQQGSSMNTRLSNSFHIPPASLQAIPYIMLIFLVPLYDSFLV 428

Query: 301 PFARTLTRTEAGITHLQRIGIGLLLSILAMAIAALVETKRK--GVADAHPDAHPLPITFL 360
           PFAR LT   +GI  L RIGIGL LS  +M  AA++E KR+   V D         ++  
Sbjct: 429 PFARKLTGHNSGIPPLTRIGIGLFLSTFSMVSAAMLEKKRRDSSVLDGR------ILSIF 488

Query: 361 WIAFQYLFLGSADLFTLAGLLEFFFTEAPATMRSLATALSWASLAVGYYLSSVIVSVVNR 420
           WI  Q+L  G +++FT  GL+EFF+ ++   M S   AL++ S + G+Y SSV+VSVVN+
Sbjct: 489 WITPQFLIFGISEMFTAVGLIEFFYKQSAKGMESFLMALTYCSYSFGFYFSSVLVSVVNK 548

Query: 421 VTAKASHTP-WLSGHNINHYRLSNFYWLMCALSAFNFLHYLFWALKYKYRSTQGN 457
           +T+ +  +  WL  +++N  RL  FYWL+  LS  NFL YLFW+ ++  +S++ N
Sbjct: 549 ITSTSVDSKGWLGENDLNKDRLDLFYWLLAVLSLLNFLSYLFWS-RWNIKSSRRN 581

BLAST of Cla97C08G151790 vs. ExPASy Swiss-Prot
Match: Q56XQ6 (Protein NRT1/ PTR FAMILY 4.4 OS=Arabidopsis thaliana OX=3702 GN=NPF4.4 PE=2 SV=1)

HSP 1 Score: 287.7 bits (735), Expect = 2.3e-76
Identity = 183/437 (41.88%), Postives = 266/437 (60.87%), Query Frame = 0

Query: 20  CKEVGGREAAVLFMGLYLVALGVGGIKGSLPAHGAEQFDEGSPQGRKRRSSFFNYFVFSL 79
           C E  G +AA L+  L LVALG G +K ++ +HGA QF     +  ++ SSFFN   F+ 
Sbjct: 150 CVEANGYKAATLYTALCLVALGSGCLKPNIISHGANQFQR---KDLRKLSSFFNAAYFAF 209

Query: 80  SCGALIAVTFVVWVEDNLGWQWGFGISTISIFLSIPLFLAGSPFYRNKIPTGSPLTTILK 139
           S G LIA+T +VWV+ + G   GFG+S   +   +   +AG+ FYRNK P+GS  T I +
Sbjct: 210 SMGQLIALTLLVWVQTHSGMDVGFGVSAAVMAAGMISLVAGTSFYRNKPPSGSIFTPIAQ 269

Query: 140 VLVAATLNRESSKKTSTNAVASLARSPSTSTPKLINKELTQNNTTCPTQSLKFLNKA--- 199
           V VAA   R+    ++ N V      PST   ++  K L  +N        +FL+KA   
Sbjct: 270 VFVAAITKRKQICPSNPNMV----HQPSTDLVRV--KPLLHSN------KFRFLDKACIK 329

Query: 200 VQNPAFH-PSLKCTTQQLEDVKIVLKVLPIFACTIILNSCLAQLSTFSIEQASTMNTKIA 259
            Q  A   P   CT +Q+  VKI+L V+PIFACTII N+ LAQL TFS++Q S+MNT I 
Sbjct: 330 TQGKAMESPWRLCTIEQVHQVKILLSVIPIFACTIIFNTILAQLQTFSVQQGSSMNTHIT 389

Query: 260 -SLKLPPASLPIFPVLFIIVLAPLYDHLILPFARTLTRTEAGITHLQRIGIGLLLSILAM 319
            + ++PPASL   P + +I   PLY+   +P AR LT  ++GI+ LQRIG GL L+  +M
Sbjct: 390 KTFQIPPASLQAIPYIILIFFVPLYETFFVPLARKLTGNDSGISPLQRIGTGLFLATFSM 449

Query: 320 AIAALVETKRKGVADAHPDAHPLPITFLWIAFQYLFLGSADLFTLAGLLEFFFTEAPATM 379
             AALVE KR+   ++  + + + ++  WIA Q+L  G +++FT  GL+EFF+ ++  +M
Sbjct: 450 VAAALVEKKRR---ESFLEQNVM-LSIFWIAPQFLIFGLSEMFTAVGLVEFFYKQSSQSM 509

Query: 380 RSLATALSWASLAVGYYLSSVIVSVVNRVTA---KASHTPWLSGHNINHYRLSNFYWLMC 439
           +S  TA+++ S + G+YLSSV+VS VNRVT+     +   WL  +++N  RL +FYWL+ 
Sbjct: 510 QSFLTAMTYCSYSFGFYLSSVLVSTVNRVTSSNGSGTKEGWLGDNDLNKDRLDHFYWLLA 567

Query: 440 ALSAFNFLHYLFWALKY 449
           +LS  NF +YLFW+  Y
Sbjct: 570 SLSFINFFNYLFWSRWY 567

BLAST of Cla97C08G151790 vs. ExPASy TrEMBL
Match: A0A0A0KHD2 (Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_6G404120 PE=3 SV=1)

HSP 1 Score: 803.9 bits (2075), Expect = 3.5e-229
Identity = 416/460 (90.43%), Postives = 439/460 (95.43%), Query Frame = 0

Query: 1   MLTVQAKVSSSCDEGNSKGCKEVGGREAAVLFMGLYLVALGVGGIKGSLPAHGAEQFDEG 60
           MLTVQAKVSSSC+EG+SKGCKEVGG+E AVLF+GLYLVALGVGGIKGSLPAHGAEQFDE 
Sbjct: 1   MLTVQAKVSSSCEEGSSKGCKEVGGKEMAVLFIGLYLVALGVGGIKGSLPAHGAEQFDES 60

Query: 61  SPQGRKRRSSFFNYFVFSLSCGALIAVTFVVWVEDNLGWQWGFGISTISIFLSIPLFLAG 120
           +PQGRKRRSSFFNYFVFSLSCGALIAVTFVVW+EDNLGWQWGFGISTISIFLSIPLF AG
Sbjct: 61  TPQGRKRRSSFFNYFVFSLSCGALIAVTFVVWIEDNLGWQWGFGISTISIFLSIPLFFAG 120

Query: 121 SPFYRNKIPTGSPLTTILKVLVAATLNRESSKKTSTNAVASLARSPS-TSTPK-LINKEL 180
           SPFYRNKIPTGSPLTTILKVLVAATLNR S+KK +TNAVASLARSPS TSTPK LINK+L
Sbjct: 121 SPFYRNKIPTGSPLTTILKVLVAATLNRRSNKKPATNAVASLARSPSTTSTPKQLINKDL 180

Query: 181 TQNNTTCPTQSLKFLNKAVQNPAFHPSLKCTTQQLEDVKIVLKVLPIFACTIILNSCLAQ 240
           T+NN T PTQSLKFLNKAVQNP FHPSLKCTTQQLEDVKIV+KVLPIFACTIILNSCLAQ
Sbjct: 181 TENNVTTPTQSLKFLNKAVQNPPFHPSLKCTTQQLEDVKIVIKVLPIFACTIILNSCLAQ 240

Query: 241 LSTFSIEQASTMNTKIASLKLPPASLPIFPVLFIIVLAPLYDHLILPFARTLTRTEAGIT 300
           LSTFSIEQA+TMNTKI SLK+PPASLP+FPVLFII+LAPLYDHLI+PFAR+LTRTEAGIT
Sbjct: 241 LSTFSIEQAATMNTKIYSLKIPPASLPVFPVLFIIILAPLYDHLIIPFARSLTRTEAGIT 300

Query: 301 HLQRIGIGLLLSILAMAIAALVETKRKGVADAHPDAH-PLPITFLWIAFQYLFLGSADLF 360
           HLQRIG+GLLLS+LAMA+AALVETKRK VADA+PD H PLPITFLWIAFQYLFLGSADLF
Sbjct: 301 HLQRIGVGLLLSVLAMAVAALVETKRKAVADANPDVHGPLPITFLWIAFQYLFLGSADLF 360

Query: 361 TLAGLLEFFFTEAPATMRSLATALSWASLAVGYYLSSVIVSVVNRVTAKASHTPWLSGHN 420
           TLAGLLEFFFTEAP TMRSLATALSWASLAVGYYLSSVIVSVVN V+AKA H PWLSGHN
Sbjct: 361 TLAGLLEFFFTEAPTTMRSLATALSWASLAVGYYLSSVIVSVVNHVSAKAWHRPWLSGHN 420

Query: 421 INHYRLSNFYWLMCALSAFNFLHYLFWALKYKYRSTQGNK 458
           INHYRLSNFYW+MC LS  NFLHYLFWALKYKYRSTQGNK
Sbjct: 421 INHYRLSNFYWVMCVLSGLNFLHYLFWALKYKYRSTQGNK 460

BLAST of Cla97C08G151790 vs. ExPASy TrEMBL
Match: A0A1P8SN46 (NRT1.2-like nitrate transporter OS=Cucumis sativus OX=3659 GN=NRT1.2C PE=2 SV=1)

HSP 1 Score: 803.9 bits (2075), Expect = 3.5e-229
Identity = 416/460 (90.43%), Postives = 439/460 (95.43%), Query Frame = 0

Query: 1   MLTVQAKVSSSCDEGNSKGCKEVGGREAAVLFMGLYLVALGVGGIKGSLPAHGAEQFDEG 60
           MLTVQAKVSSSC+EG+SKGCKEVGG+E AVLF+GLYLVALGVGGIKGSLPAHGAEQFDE 
Sbjct: 116 MLTVQAKVSSSCEEGSSKGCKEVGGKEMAVLFIGLYLVALGVGGIKGSLPAHGAEQFDES 175

Query: 61  SPQGRKRRSSFFNYFVFSLSCGALIAVTFVVWVEDNLGWQWGFGISTISIFLSIPLFLAG 120
           +PQGRKRRSSFFNYFVFSLSCGALIAVTFVVW+EDNLGWQWGFGISTISIFLSIPLF AG
Sbjct: 176 TPQGRKRRSSFFNYFVFSLSCGALIAVTFVVWIEDNLGWQWGFGISTISIFLSIPLFFAG 235

Query: 121 SPFYRNKIPTGSPLTTILKVLVAATLNRESSKKTSTNAVASLARSPS-TSTPK-LINKEL 180
           SPFYRNKIPTGSPLTTILKVLVAATLNR S+KK +TNAVASLARSPS TSTPK LINK+L
Sbjct: 236 SPFYRNKIPTGSPLTTILKVLVAATLNRRSNKKPATNAVASLARSPSTTSTPKQLINKDL 295

Query: 181 TQNNTTCPTQSLKFLNKAVQNPAFHPSLKCTTQQLEDVKIVLKVLPIFACTIILNSCLAQ 240
           T+NN T PTQSLKFLNKAVQNP FHPSLKCTTQQLEDVKIV+KVLPIFACTIILNSCLAQ
Sbjct: 296 TENNVTTPTQSLKFLNKAVQNPPFHPSLKCTTQQLEDVKIVIKVLPIFACTIILNSCLAQ 355

Query: 241 LSTFSIEQASTMNTKIASLKLPPASLPIFPVLFIIVLAPLYDHLILPFARTLTRTEAGIT 300
           LSTFSIEQA+TMNTKI SLK+PPASLP+FPVLFII+LAPLYDHLI+PFAR+LTRTEAGIT
Sbjct: 356 LSTFSIEQAATMNTKIYSLKIPPASLPVFPVLFIIILAPLYDHLIIPFARSLTRTEAGIT 415

Query: 301 HLQRIGIGLLLSILAMAIAALVETKRKGVADAHPDAH-PLPITFLWIAFQYLFLGSADLF 360
           HLQRIG+GLLLS+LAMA+AALVETKRK VADA+PD H PLPITFLWIAFQYLFLGSADLF
Sbjct: 416 HLQRIGVGLLLSVLAMAVAALVETKRKAVADANPDVHGPLPITFLWIAFQYLFLGSADLF 475

Query: 361 TLAGLLEFFFTEAPATMRSLATALSWASLAVGYYLSSVIVSVVNRVTAKASHTPWLSGHN 420
           TLAGLLEFFFTEAP TMRSLATALSWASLAVGYYLSSVIVSVVN V+AKA H PWLSGHN
Sbjct: 476 TLAGLLEFFFTEAPTTMRSLATALSWASLAVGYYLSSVIVSVVNHVSAKAWHRPWLSGHN 535

Query: 421 INHYRLSNFYWLMCALSAFNFLHYLFWALKYKYRSTQGNK 458
           INHYRLSNFYW+MC LS  NFLHYLFWALKYKYRSTQGNK
Sbjct: 536 INHYRLSNFYWVMCVLSGLNFLHYLFWALKYKYRSTQGNK 575

BLAST of Cla97C08G151790 vs. ExPASy TrEMBL
Match: A0A5D3BLL0 (Protein NRT1/ PTR FAMILY 4.6-like OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold360G00950 PE=3 SV=1)

HSP 1 Score: 792.0 bits (2044), Expect = 1.4e-225
Identity = 413/460 (89.78%), Postives = 438/460 (95.22%), Query Frame = 0

Query: 1   MLTVQAKVSSSCDEGNSKGCKEVGGREAAVLFMGLYLVALGVGGIKGSLPAHGAEQFDEG 60
           MLTVQAKVSSSC+ G +KGCKEVGG+E AVLF+GLYLVALGVGGIKGSLPAHGAEQFDE 
Sbjct: 116 MLTVQAKVSSSCEAG-TKGCKEVGGKEVAVLFIGLYLVALGVGGIKGSLPAHGAEQFDES 175

Query: 61  SPQGRKRRSSFFNYFVFSLSCGALIAVTFVVWVEDNLGWQWGFGISTISIFLSIPLFLAG 120
           + QGRKRRSSFFNYFVFSLSCGAL+AVTFVVWVEDNLGWQWGFGISTIS FLSIPLF AG
Sbjct: 176 TTQGRKRRSSFFNYFVFSLSCGALVAVTFVVWVEDNLGWQWGFGISTISTFLSIPLFFAG 235

Query: 121 SPFYRNKIPTGSPLTTILKVLVAATLNRESSKKTSTNAVASLARSPS-TSTPK-LINKEL 180
           SPFYRNKIPTGSPLTTILKVLVAATLNR S+KK +TNAVASLARSPS TSTPK LINK+L
Sbjct: 236 SPFYRNKIPTGSPLTTILKVLVAATLNRRSNKKPATNAVASLARSPSTTSTPKPLINKDL 295

Query: 181 TQNNTTCPTQSLKFLNKAVQNPAFHPSLKCTTQQLEDVKIVLKVLPIFACTIILNSCLAQ 240
           T++NTT PTQSLKFLNKAVQNP FHPS+KCTTQQLEDVKIV+KVLPIFACTIILNSCLAQ
Sbjct: 296 TESNTTTPTQSLKFLNKAVQNPPFHPSVKCTTQQLEDVKIVIKVLPIFACTIILNSCLAQ 355

Query: 241 LSTFSIEQASTMNTKIASLKLPPASLPIFPVLFIIVLAPLYDHLILPFARTLTRTEAGIT 300
           LSTFSIEQA+TMNTKI+SLK+PPASLP+FPVLFIIVLAPLYDHLI+PFAR+LTRTEAGIT
Sbjct: 356 LSTFSIEQAATMNTKISSLKIPPASLPVFPVLFIIVLAPLYDHLIIPFARSLTRTEAGIT 415

Query: 301 HLQRIGIGLLLSILAMAIAALVETKRKGVADAHPDAH-PLPITFLWIAFQYLFLGSADLF 360
           HLQRIG+GLLLS+LAMA+AALVETKRK VADA+PD   PLPITFLWIAFQYLFLGSADLF
Sbjct: 416 HLQRIGVGLLLSVLAMAVAALVETKRKAVADANPDVQGPLPITFLWIAFQYLFLGSADLF 475

Query: 361 TLAGLLEFFFTEAPATMRSLATALSWASLAVGYYLSSVIVSVVNRVTAKASHTPWLSGHN 420
           TLAGLLEFFFTEAPATMRSLATALSWASLAVGYYLSSVIVSVVN V+AKA H PWLSGHN
Sbjct: 476 TLAGLLEFFFTEAPATMRSLATALSWASLAVGYYLSSVIVSVVNHVSAKAWHRPWLSGHN 535

Query: 421 INHYRLSNFYWLMCALSAFNFLHYLFWALKYKYRSTQGNK 458
           INHYRLSNFYW+MC LS FNFLHYLFWALKYKYRSTQGNK
Sbjct: 536 INHYRLSNFYWVMCVLSGFNFLHYLFWALKYKYRSTQGNK 574

BLAST of Cla97C08G151790 vs. ExPASy TrEMBL
Match: A0A1S3C3A1 (protein NRT1/ PTR FAMILY 4.6-like OS=Cucumis melo OX=3656 GN=LOC103496401 PE=3 SV=1)

HSP 1 Score: 792.0 bits (2044), Expect = 1.4e-225
Identity = 413/460 (89.78%), Postives = 438/460 (95.22%), Query Frame = 0

Query: 1   MLTVQAKVSSSCDEGNSKGCKEVGGREAAVLFMGLYLVALGVGGIKGSLPAHGAEQFDEG 60
           MLTVQAKVSSSC+ G +KGCKEVGG+E AVLF+GLYLVALGVGGIKGSLPAHGAEQFDE 
Sbjct: 116 MLTVQAKVSSSCEAG-TKGCKEVGGKEVAVLFIGLYLVALGVGGIKGSLPAHGAEQFDES 175

Query: 61  SPQGRKRRSSFFNYFVFSLSCGALIAVTFVVWVEDNLGWQWGFGISTISIFLSIPLFLAG 120
           + QGRKRRSSFFNYFVFSLSCGAL+AVTFVVWVEDNLGWQWGFGISTIS FLSIPLF AG
Sbjct: 176 TTQGRKRRSSFFNYFVFSLSCGALVAVTFVVWVEDNLGWQWGFGISTISTFLSIPLFFAG 235

Query: 121 SPFYRNKIPTGSPLTTILKVLVAATLNRESSKKTSTNAVASLARSPS-TSTPK-LINKEL 180
           SPFYRNKIPTGSPLTTILKVLVAATLNR S+KK +TNAVASLARSPS TSTPK LINK+L
Sbjct: 236 SPFYRNKIPTGSPLTTILKVLVAATLNRRSNKKPATNAVASLARSPSTTSTPKPLINKDL 295

Query: 181 TQNNTTCPTQSLKFLNKAVQNPAFHPSLKCTTQQLEDVKIVLKVLPIFACTIILNSCLAQ 240
           T++NTT PTQSLKFLNKAVQNP FHPS+KCTTQQLEDVKIV+KVLPIFACTIILNSCLAQ
Sbjct: 296 TESNTTTPTQSLKFLNKAVQNPPFHPSVKCTTQQLEDVKIVIKVLPIFACTIILNSCLAQ 355

Query: 241 LSTFSIEQASTMNTKIASLKLPPASLPIFPVLFIIVLAPLYDHLILPFARTLTRTEAGIT 300
           LSTFSIEQA+TMNTKI+SLK+PPASLP+FPVLFIIVLAPLYDHLI+PFAR+LTRTEAGIT
Sbjct: 356 LSTFSIEQAATMNTKISSLKIPPASLPVFPVLFIIVLAPLYDHLIIPFARSLTRTEAGIT 415

Query: 301 HLQRIGIGLLLSILAMAIAALVETKRKGVADAHPDAH-PLPITFLWIAFQYLFLGSADLF 360
           HLQRIG+GLLLS+LAMA+AALVETKRK VADA+PD   PLPITFLWIAFQYLFLGSADLF
Sbjct: 416 HLQRIGVGLLLSVLAMAVAALVETKRKAVADANPDVQGPLPITFLWIAFQYLFLGSADLF 475

Query: 361 TLAGLLEFFFTEAPATMRSLATALSWASLAVGYYLSSVIVSVVNRVTAKASHTPWLSGHN 420
           TLAGLLEFFFTEAPATMRSLATALSWASLAVGYYLSSVIVSVVN V+AKA H PWLSGHN
Sbjct: 476 TLAGLLEFFFTEAPATMRSLATALSWASLAVGYYLSSVIVSVVNHVSAKAWHRPWLSGHN 535

Query: 421 INHYRLSNFYWLMCALSAFNFLHYLFWALKYKYRSTQGNK 458
           INHYRLSNFYW+MC LS FNFLHYLFWALKYKYRSTQGNK
Sbjct: 536 INHYRLSNFYWVMCVLSGFNFLHYLFWALKYKYRSTQGNK 574

BLAST of Cla97C08G151790 vs. ExPASy TrEMBL
Match: A0A6J1G2M6 (protein NRT1/ PTR FAMILY 4.6-like OS=Cucurbita moschata OX=3662 GN=LOC111450266 PE=3 SV=1)

HSP 1 Score: 688.7 bits (1776), Expect = 1.6e-194
Identity = 357/457 (78.12%), Postives = 404/457 (88.40%), Query Frame = 0

Query: 1   MLTVQAKVSSSCDEGNSKGCKEVGGREAAVLFMGLYLVALGVGGIKGSLPAHGAEQFDEG 60
           +LTV+AK +++                AAV+F+GLYLVA+GVGGIKGSLP HGAEQF+EG
Sbjct: 116 ILTVEAKAATAA--------------SAAVVFVGLYLVAVGVGGIKGSLPTHGAEQFEEG 175

Query: 61  SPQGRKRRSSFFNYFVFSLSCGALIAVTFVVWVEDNLGWQWGFGISTISIFLSIPLFLAG 120
           + +GR+ RSSFFNYFVFSLSCGA++AVT VVW+EDNLGW+WGFGISTI+I +SIPLFLAG
Sbjct: 176 TAEGRRGRSSFFNYFVFSLSCGAVVAVTLVVWMEDNLGWEWGFGISTIAILVSIPLFLAG 235

Query: 121 SPFYRNKIPTGSPLTTILKVLVAATLNRESSKKTSTNAVASLARSPSTSTPKLINKELTQ 180
           S FYRNKIP G+PLTTILKVLVAATLN  S  KT++NAVAS+ RSPST+TPK   KE T 
Sbjct: 236 SCFYRNKIPAGAPLTTILKVLVAATLNGRSRNKTASNAVASMTRSPSTTTPK-PTKEPT- 295

Query: 181 NNTTCPTQSLKFLNKAVQNPAFHPSLKCTTQQLEDVKIVLKVLPIFACTIILNSCLAQLS 240
           + T  PTQ+LKFLNKA+Q P FHPSL CTTQQLEDVK+VLK+LPIFACTIILNSCLAQLS
Sbjct: 296 HTTPPPTQTLKFLNKAIQTPPFHPSLSCTTQQLEDVKVVLKLLPIFACTIILNSCLAQLS 355

Query: 241 TFSIEQASTMNTKIASLKLPPASLPIFPVLFIIVLAPLYDHLILPFARTLTRTEAGITHL 300
           TFS+EQASTMNTKI S+KLPPASLPIFP+LFII+LAPLY+HLI+PFARTLTRTE+GITHL
Sbjct: 356 TFSVEQASTMNTKIGSIKLPPASLPIFPILFIILLAPLYNHLIIPFARTLTRTESGITHL 415

Query: 301 QRIGIGLLLSILAMAIAALVETKRKGVADAHPDAHPLPITFLWIAFQYLFLGSADLFTLA 360
           QRIG+GLLLSI+AMAIAAL+E KRK +ADAHP AHPLPITFLWI FQYLFLGSADLFTLA
Sbjct: 416 QRIGVGLLLSIVAMAIAALIEVKRKAIADAHPAAHPLPITFLWIGFQYLFLGSADLFTLA 475

Query: 361 GLLEFFFTEAPATMRSLATALSWASLAVGYYLSSVIVSVVNRVTAKASHTPWLSGHNINH 420
           GLLEFFFTEAP+TMRSLATALSWASLAVGYYLSSVIVS+VNRVTAK+SH+PWLS  +INH
Sbjct: 476 GLLEFFFTEAPSTMRSLATALSWASLAVGYYLSSVIVSLVNRVTAKSSHSPWLSAQDINH 535

Query: 421 YRLSNFYWLMCALSAFNFLHYLFWALKYKYRSTQGNK 458
           Y L  FYWLMCALSA NFLH+LFWA+KYKYRST  +K
Sbjct: 536 YDLHKFYWLMCALSALNFLHFLFWAIKYKYRSTLASK 556

BLAST of Cla97C08G151790 vs. TAIR 10
Match: AT1G69850.1 (nitrate transporter 1:2 )

HSP 1 Score: 589.0 bits (1517), Expect = 3.4e-168
Identity = 316/473 (66.81%), Postives = 380/473 (80.34%), Query Frame = 0

Query: 1   MLTVQAKVSS----SCDEGNSKGCKEVGGREAAVLFMGLYLVALGVGGIKGSLPAHGAEQ 60
           +LT+QA+  S    SCD   S  C+EV G +AA+LF+GLYLVALGVGGIKGSL +HGAEQ
Sbjct: 113 ILTIQARTPSLMPPSCD---SPTCEEVSGSKAAMLFVGLYLVALGVGGIKGSLASHGAEQ 172

Query: 61  FDEGSPQGRKRRSSFFNYFVFSLSCGALIAVTFVVWVEDNLGWQWGFGISTISIFLSIPL 120
           FDE +P+GRK+RS+FFNYFVF L+CGAL+AVTFVVW+EDN GW+WGFG+STI+IF+SI +
Sbjct: 173 FDESTPKGRKQRSTFFNYFVFCLACGALVAVTFVVWLEDNKGWEWGFGVSTIAIFVSILI 232

Query: 121 FLAGSPFYRNKIPTGSPLTTILKVLVAATLNRESSKKTSTNAVASLARSPSTSTPKLINK 180
           FL+GS FYRNKIP GSPLTTILKVL+AA++ +  S  +S+NAVAS++ SPS        K
Sbjct: 233 FLSGSRFYRNKIPCGSPLTTILKVLLAASV-KCCSSGSSSNAVASMSVSPSNHCVSKGKK 292

Query: 181 EL-TQNNTTCP------------TQSLKFLNKAVQNPAFHPSLKCTTQQLEDVKIVLKVL 240
           E+ +Q     P            T SLK LN A      H  L+CT QQ+EDVKIVLK+L
Sbjct: 293 EVESQGELEKPRQEEALPPRAQLTNSLKVLNGAADEKPVHRLLECTVQQVEDVKIVLKML 352

Query: 241 PIFACTIILNSCLAQLSTFSIEQASTMNTKIASLKLPPASLPIFPVLFIIVLAPLYDHLI 300
           PIFACTI+LN CLAQLSTFS++QA++MNTKI SLK+PPASLPIFPV+FI++LAP+YDHLI
Sbjct: 353 PIFACTIMLNCCLAQLSTFSVQQAASMNTKIGSLKIPPASLPIFPVVFIMILAPIYDHLI 412

Query: 301 LPFARTLTRTEAGITHLQRIGIGLLLSILAMAIAALVETKRKGVADAH---PDAHPLPIT 360
           +PFAR  T+TE G+THLQRIG+GL+LSILAMA+AALVE KRKGVA           LP+T
Sbjct: 413 IPFARKATKTETGVTHLQRIGVGLVLSILAMAVAALVEIKRKGVAKDSGLLDSKETLPVT 472

Query: 361 FLWIAFQYLFLGSADLFTLAGLLEFFFTEAPATMRSLATALSWASLAVGYYLSSVIVSVV 420
           FLWIA QYLFLGSADLFTLAGLLE+FFTEAP++MRSLAT+LSWASLA+GYYLSSVIVS+V
Sbjct: 473 FLWIALQYLFLGSADLFTLAGLLEYFFTEAPSSMRSLATSLSWASLAMGYYLSSVIVSIV 532

Query: 421 NRVTAKASHTPWLSGHNINHYRLSNFYWLMCALSAFNFLHYLFWALKYKYRST 454
           N +T  + +TPWL G +IN Y+L  FYWLMC LSA NFLHYLFWA++YKYRST
Sbjct: 533 NSITGSSGNTPWLRGKSINRYKLDYFYWLMCVLSAANFLHYLFWAMRYKYRST 581

BLAST of Cla97C08G151790 vs. TAIR 10
Match: AT1G27040.2 (Major facilitator superfamily protein )

HSP 1 Score: 552.4 bits (1422), Expect = 3.5e-157
Identity = 295/460 (64.13%), Postives = 360/460 (78.26%), Query Frame = 0

Query: 1   MLTVQAKVSS----SCDEGNSKGCKEVGGREAAVLFMGLYLVALGVGGIKGSLPAHGAEQ 60
           +LT+QA+  S     C    +  C+ VGG +AA LF+GLYLV+LG+GGIKGSLP+HGAEQ
Sbjct: 111 LLTIQARRPSLMPPPCKSSAALRCEVVGGSKAAFLFVGLYLVSLGIGGIKGSLPSHGAEQ 170

Query: 61  FDEGSPQGRKRRSSFFNYFVFSLSCGALIAVTFVVWVEDNLGWQWGFGISTISIFLSIPL 120
           FDEG+P+GRK+RS+FFNY+VF LSCGAL+AVTFVVW+EDN GW+WGFG+STISIFLSI +
Sbjct: 171 FDEGTPKGRKQRSTFFNYYVFCLSCGALVAVTFVVWIEDNKGWEWGFGVSTISIFLSILV 230

Query: 121 FLAGSPFYRNKIPTGSPLTTILKVLVAATLNRESSKKTSTNAVASLARSPSTSTPKLINK 180
           FL GS FY+NKIP GSPLTTI KVL+AA++   SSK +S +  +   +S         ++
Sbjct: 231 FLLGSRFYKNKIPRGSPLTTIFKVLLAASIVSCSSKTSSNHFTSREVQSE--------HE 290

Query: 181 ELTQNNTTCPTQSLKFLNKAVQNPAFHPSLKCTTQQLEDVKIVLKVLPIFACTIILNSCL 240
           E T + +   T SL  LNKA++    H  L+CT QQ+EDVKIVLK+LPIF CTI+LN CL
Sbjct: 291 EKTPSQSL--TNSLTCLNKAIEGKTHHIWLECTVQQVEDVKIVLKMLPIFGCTIMLNCCL 350

Query: 241 AQLSTFSIEQASTMNTKIASLKLPPASLPIFPVLFIIVLAPLYDHLILPFARTLTRTEAG 300
           AQLST+S+ QA+TMN KI +  +P ASLP+FPV+F+++LAP YDHLI+PFAR +T++E G
Sbjct: 351 AQLSTYSVHQAATMNRKIVNFNVPSASLPVFPVVFMLILAPTYDHLIIPFARKVTKSEIG 410

Query: 301 ITHLQRIGIGLLLSILAMAIAALVETKRKGV---ADAHPDAHPLPITFLWIAFQYLFLGS 360
           ITHLQRIG+GL+LSI+AMA+AALVE KRK V   A        LPITFLWIA QYLFLGS
Sbjct: 411 ITHLQRIGVGLVLSIVAMAVAALVELKRKQVAREAGLLDSEETLPITFLWIALQYLFLGS 470

Query: 361 ADLFTLAGLLEFFFTEAPATMRSLATALSWASLAVGYYLSSVIVSVVNRVTAKASHTPWL 420
           ADLFTLAGLLEFFFTEAP++MRSLAT+LSWASLA+GYYLSSV+V +VNRVT  A  +PWL
Sbjct: 471 ADLFTLAGLLEFFFTEAPSSMRSLATSLSWASLALGYYLSSVMVPIVNRVTKSAGQSPWL 530

Query: 421 SGHNINHYRLSNFYWLMCALSAFNFLHYLFWALKYKYRST 454
            G  +N  RL  FYWLMC LS  NFLHYLFWA +YKY ST
Sbjct: 531 -GEKLNRNRLDLFYWLMCVLSVVNFLHYLFWAKRYKYIST 559

BLAST of Cla97C08G151790 vs. TAIR 10
Match: AT1G27040.1 (Major facilitator superfamily protein )

HSP 1 Score: 552.4 bits (1422), Expect = 3.5e-157
Identity = 295/460 (64.13%), Postives = 360/460 (78.26%), Query Frame = 0

Query: 1   MLTVQAKVSS----SCDEGNSKGCKEVGGREAAVLFMGLYLVALGVGGIKGSLPAHGAEQ 60
           +LT+QA+  S     C    +  C+ VGG +AA LF+GLYLV+LG+GGIKGSLP+HGAEQ
Sbjct: 115 LLTIQARRPSLMPPPCKSSAALRCEVVGGSKAAFLFVGLYLVSLGIGGIKGSLPSHGAEQ 174

Query: 61  FDEGSPQGRKRRSSFFNYFVFSLSCGALIAVTFVVWVEDNLGWQWGFGISTISIFLSIPL 120
           FDEG+P+GRK+RS+FFNY+VF LSCGAL+AVTFVVW+EDN GW+WGFG+STISIFLSI +
Sbjct: 175 FDEGTPKGRKQRSTFFNYYVFCLSCGALVAVTFVVWIEDNKGWEWGFGVSTISIFLSILV 234

Query: 121 FLAGSPFYRNKIPTGSPLTTILKVLVAATLNRESSKKTSTNAVASLARSPSTSTPKLINK 180
           FL GS FY+NKIP GSPLTTI KVL+AA++   SSK +S +  +   +S         ++
Sbjct: 235 FLLGSRFYKNKIPRGSPLTTIFKVLLAASIVSCSSKTSSNHFTSREVQSE--------HE 294

Query: 181 ELTQNNTTCPTQSLKFLNKAVQNPAFHPSLKCTTQQLEDVKIVLKVLPIFACTIILNSCL 240
           E T + +   T SL  LNKA++    H  L+CT QQ+EDVKIVLK+LPIF CTI+LN CL
Sbjct: 295 EKTPSQSL--TNSLTCLNKAIEGKTHHIWLECTVQQVEDVKIVLKMLPIFGCTIMLNCCL 354

Query: 241 AQLSTFSIEQASTMNTKIASLKLPPASLPIFPVLFIIVLAPLYDHLILPFARTLTRTEAG 300
           AQLST+S+ QA+TMN KI +  +P ASLP+FPV+F+++LAP YDHLI+PFAR +T++E G
Sbjct: 355 AQLSTYSVHQAATMNRKIVNFNVPSASLPVFPVVFMLILAPTYDHLIIPFARKVTKSEIG 414

Query: 301 ITHLQRIGIGLLLSILAMAIAALVETKRKGV---ADAHPDAHPLPITFLWIAFQYLFLGS 360
           ITHLQRIG+GL+LSI+AMA+AALVE KRK V   A        LPITFLWIA QYLFLGS
Sbjct: 415 ITHLQRIGVGLVLSIVAMAVAALVELKRKQVAREAGLLDSEETLPITFLWIALQYLFLGS 474

Query: 361 ADLFTLAGLLEFFFTEAPATMRSLATALSWASLAVGYYLSSVIVSVVNRVTAKASHTPWL 420
           ADLFTLAGLLEFFFTEAP++MRSLAT+LSWASLA+GYYLSSV+V +VNRVT  A  +PWL
Sbjct: 475 ADLFTLAGLLEFFFTEAPSSMRSLATSLSWASLALGYYLSSVMVPIVNRVTKSAGQSPWL 534

Query: 421 SGHNINHYRLSNFYWLMCALSAFNFLHYLFWALKYKYRST 454
            G  +N  RL  FYWLMC LS  NFLHYLFWA +YKY ST
Sbjct: 535 -GEKLNRNRLDLFYWLMCVLSVVNFLHYLFWAKRYKYIST 563

BLAST of Cla97C08G151790 vs. TAIR 10
Match: AT5G62730.1 (Major facilitator superfamily protein )

HSP 1 Score: 507.3 bits (1305), Expect = 1.3e-143
Identity = 268/435 (61.61%), Postives = 328/435 (75.40%), Query Frame = 0

Query: 31  LFMGLYLVALGVGGIKGSLPAHGAEQFDEGSPQGRKRRSSFFNYFVFSLSCGALIAVTFV 90
           LF+GLYLVALGVGGIKGSLP HGAEQFDE +  GR++RS FFNYF+FSLSCGALIAVT V
Sbjct: 162 LFVGLYLVALGVGGIKGSLPPHGAEQFDEETSSGRRQRSFFFNYFIFSLSCGALIAVTVV 221

Query: 91  VWVEDNLGWQWGFGISTISIFLSIPLFLAGSPFYRNKIPTGSPLTTILKVLVAATLNRES 150
           VW+EDN GW +GFG+ST +I +S+P+FLAGS  YR K+P+GSP+TT+ KVL AA   +  
Sbjct: 222 VWLEDNKGWSYGFGVSTAAILISVPVFLAGSRVYRLKVPSGSPITTLFKVLTAALYAKYK 281

Query: 151 SKKTSTNAVASLARSPSTSTPKLINKELTQNNTTCPTQSL-KFLNKAVQNPAFHP-SLKC 210
            ++TS   V    R+         +  +T+ N       L  FL + V+     P  L+C
Sbjct: 282 KRRTSRIVVTCHTRND-------CDDSVTKQNCDGDDGFLGSFLGEVVRERESLPRPLRC 341

Query: 211 TTQQLEDVKIVLKVLPIFACTIILNSCLAQLSTFSIEQASTMNTKIASLKLPPASLPIFP 270
           T +Q++DVKIV+K+LPIF  TI+LN CLAQLSTFS++QASTMNTK+ S  +PPA+LP+FP
Sbjct: 342 TEEQVKDVKIVIKILPIFMSTIMLNCCLAQLSTFSVQQASTMNTKLGSFTVPPAALPVFP 401

Query: 271 VLFIIVLAPLYDHLILPFARTLTRTEAGITHLQRIGIGLLLSILAMAIAALVETKRKGVA 330
           V+F+++LAP Y+HL+LP AR  T+TE GITHLQRIG GL+LSI+AMA+AALVETKRK V 
Sbjct: 402 VVFMMILAPTYNHLLLPLARKSTKTETGITHLQRIGTGLVLSIVAMAVAALVETKRKHVV 461

Query: 331 --------DAHPDAHPLPITFLWIAFQYLFLGSADLFTLAGLLEFFFTEAPATMRSLATA 390
                    +   + PLPITFLW+A QY+FLGSADLFTLAG++EFFFTEAP+TMRSLAT+
Sbjct: 462 VSCCSNNNSSSYSSSPLPITFLWVAIQYVFLGSADLFTLAGMMEFFFTEAPSTMRSLATS 521

Query: 391 LSWASLAVGYYLSSVIVSVVNRVTAKASHTPWLSGHNINHYRLSNFYWLMCALSAFNFLH 450
           LSWASLA+GYY SSV+VS VN VT    H PWL G N+N Y L  FYWLMC LS  NFLH
Sbjct: 522 LSWASLAMGYYFSSVLVSAVNFVTGLNHHNPWLLGENLNQYHLERFYWLMCVLSGINFLH 581

Query: 451 YLFWALKYKYRSTQG 456
           YLFWA +Y YRS QG
Sbjct: 582 YLFWASRYVYRSNQG 589

BLAST of Cla97C08G151790 vs. TAIR 10
Match: AT1G59740.1 (Major facilitator superfamily protein )

HSP 1 Score: 294.3 bits (752), Expect = 1.7e-79
Identity = 186/475 (39.16%), Postives = 280/475 (58.95%), Query Frame = 0

Query: 1   MLTVQAKVSS----SCDEGNSKGCKEVGGREAAVLFMGLYLVALGVGGIKGSLPAHGAEQ 60
           +L+VQA +       C+    + C+E  G +A + FM LYLVALG G +K ++ AHGA+Q
Sbjct: 129 LLSVQAHLPQLKPPKCNPLIDQTCEEAKGFKAMIFFMALYLVALGSGCVKPNMIAHGADQ 188

Query: 61  FDEGSPQGRKRRSSFFNYFVFSLSCGALIAVTFVVWVEDNLGWQWGFGISTISIFLSIPL 120
           F +  P+  KR SS+FN   F+ S G LIA+T +VWV+ + G   GFG+S  ++ + I  
Sbjct: 189 FSQSHPKQSKRLSSYFNAAYFAFSMGELIALTLLVWVQTHSGMDIGFGVSAAAMTMGIIS 248

Query: 121 FLAGSPFYRNKIPTGSPLTTILKVLVAATLNRESSKKTSTNAVASLARSPSTSTPKLINK 180
            ++G+ ++RNK P  S  T I  V+VAA L R+ +               S S P++++ 
Sbjct: 249 LVSGTMYFRNKRPRRSIFTPIAHVIVAAILKRKLA---------------SPSDPRMLHG 308

Query: 181 ELTQNNTTCPTQSL------KFLNKAV-----QNPAFHPSLKCTTQQLEDVKIVLKVLPI 240
           +    N   P+ +L      +FL+KA       N    P   CT  Q+E VK ++ ++PI
Sbjct: 309 DHHVANDVVPSSTLPHTPRFRFLDKACIKIQDTNTKESPWRLCTVTQVEQVKTLISLVPI 368

Query: 241 FACTIILNSCLAQLSTFSIEQASTMNTKIA-SLKLPPASLPIFPVLFIIVLAPLYDHLIL 300
           FA TI+ N+ LAQL TFS++Q S+MNT+++ S  +PPASL   P + +I L PLYD  ++
Sbjct: 369 FASTIVFNTILAQLQTFSVQQGSSMNTRLSNSFHIPPASLQAIPYIMLIFLVPLYDSFLV 428

Query: 301 PFARTLTRTEAGITHLQRIGIGLLLSILAMAIAALVETKRK--GVADAHPDAHPLPITFL 360
           PFAR LT   +GI  L RIGIGL LS  +M  AA++E KR+   V D         ++  
Sbjct: 429 PFARKLTGHNSGIPPLTRIGIGLFLSTFSMVSAAMLEKKRRDSSVLDGR------ILSIF 488

Query: 361 WIAFQYLFLGSADLFTLAGLLEFFFTEAPATMRSLATALSWASLAVGYYLSSVIVSVVNR 420
           WI  Q+L  G +++FT  GL+EFF+ ++   M S   AL++ S + G+Y SSV+VSVVN+
Sbjct: 489 WITPQFLIFGISEMFTAVGLIEFFYKQSAKGMESFLMALTYCSYSFGFYFSSVLVSVVNK 548

Query: 421 VTAKASHTP-WLSGHNINHYRLSNFYWLMCALSAFNFLHYLFWALKYKYRSTQGN 457
           +T+ +  +  WL  +++N  RL  FYWL+  LS  NFL YLFW+ ++  +S++ N
Sbjct: 549 ITSTSVDSKGWLGENDLNKDRLDLFYWLLAVLSLLNFLSYLFWS-RWNIKSSRRN 581

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
XP_038886228.15.3e-24095.41protein NRT1/ PTR FAMILY 4.6-like [Benincasa hispida][more]
XP_011657481.17.2e-22990.43protein NRT1/ PTR FAMILY 4.6 [Cucumis sativus] >APY23675.1 NRT1.2-like nitrate t... [more]
XP_008456458.12.8e-22589.78PREDICTED: protein NRT1/ PTR FAMILY 4.6-like [Cucumis melo] >KAA0036440.1 protei... [more]
XP_022946066.13.4e-19478.12protein NRT1/ PTR FAMILY 4.6-like [Cucurbita moschata][more]
XP_022999670.13.7e-19379.03protein NRT1/ PTR FAMILY 4.6-like [Cucurbita maxima][more]
Match NameE-valueIdentityDescription
Q8H1574.7e-16766.81Protein NRT1/ PTR FAMILY 4.6 OS=Arabidopsis thaliana OX=3702 GN=NPF4.6 PE=1 SV=1[more]
Q8VYE44.9e-15664.13Protein NRT1/ PTR FAMILY 4.5 OS=Arabidopsis thaliana OX=3702 GN=NPF4.5 PE=2 SV=1[more]
Q9FM201.8e-14261.61Protein NRT1/ PTR FAMILY 4.7 OS=Arabidopsis thaliana OX=3702 GN=NPF4.7 PE=2 SV=2[more]
Q93VV52.4e-7839.16Protein NRT1/ PTR FAMILY 4.3 OS=Arabidopsis thaliana OX=3702 GN=NPF4.3 PE=2 SV=1[more]
Q56XQ62.3e-7641.88Protein NRT1/ PTR FAMILY 4.4 OS=Arabidopsis thaliana OX=3702 GN=NPF4.4 PE=2 SV=1[more]
Match NameE-valueIdentityDescription
A0A0A0KHD23.5e-22990.43Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_6G404120 PE=3 SV=1[more]
A0A1P8SN463.5e-22990.43NRT1.2-like nitrate transporter OS=Cucumis sativus OX=3659 GN=NRT1.2C PE=2 SV=1[more]
A0A5D3BLL01.4e-22589.78Protein NRT1/ PTR FAMILY 4.6-like OS=Cucumis melo var. makuwa OX=1194695 GN=E567... [more]
A0A1S3C3A11.4e-22589.78protein NRT1/ PTR FAMILY 4.6-like OS=Cucumis melo OX=3656 GN=LOC103496401 PE=3 S... [more]
A0A6J1G2M61.6e-19478.12protein NRT1/ PTR FAMILY 4.6-like OS=Cucurbita moschata OX=3662 GN=LOC111450266 ... [more]
Match NameE-valueIdentityDescription
AT1G69850.13.4e-16866.81nitrate transporter 1:2 [more]
AT1G27040.23.5e-15764.13Major facilitator superfamily protein [more]
AT1G27040.13.5e-15764.13Major facilitator superfamily protein [more]
AT5G62730.11.3e-14361.61Major facilitator superfamily protein [more]
AT1G59740.11.7e-7939.16Major facilitator superfamily protein [more]
InterPro
Analysis Name: InterPro Annotations of Watermelon (97103) v2.5
Date Performed: 2022-01-31
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR000109Proton-dependent oligopeptide transporter familyPFAMPF00854PTR2coord: 12..412
e-value: 8.1E-100
score: 334.4
IPR000109Proton-dependent oligopeptide transporter familyPANTHERPTHR11654OLIGOPEPTIDE TRANSPORTER-RELATEDcoord: 1..454
IPR036259MFS transporter superfamilyGENE3D1.20.1250.20MFS general substrate transporter like domainscoord: 1..456
e-value: 5.8E-134
score: 449.1
IPR036259MFS transporter superfamilySUPERFAMILY103473MFS general substrate transportercoord: 29..451
NoneNo IPR availableMOBIDB_LITEmobidb-litedisorder_predictioncoord: 1..16
NoneNo IPR availableMOBIDB_LITEmobidb-litedisorder_predictioncoord: 1..20
NoneNo IPR availablePANTHERPTHR11654:SF490PROTEIN NRT1/ PTR FAMILY 4.6-LIKEcoord: 1..454

Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
Cla97C08G151790.1Cla97C08G151790.1mRNA


GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
biological_process GO:0055085 transmembrane transport
cellular_component GO:0016021 integral component of membrane
cellular_component GO:0016020 membrane
molecular_function GO:0022857 transmembrane transporter activity