Cla97C08G148580 (gene) Watermelon (97103) v2.5

Overview
NameCla97C08G148580
Typegene
OrganismCitrullus lanatus subsp. vulgaris cv. 97103 (Watermelon (97103) v2.5)
Descriptionaspartic proteinase Asp1
LocationCla97Chr08: 16733819 .. 16737325 (+)
RNA-Seq ExpressionCla97C08G148580
SyntenyCla97C08G148580
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: exonCDSpolypeptide
Hold the cursor over a type above to highlight its positions in the sequence below.
ATGCCAACAATCACAACTCTTATCTTCTTTCTTTTAGGTCTTTCTTCAATTTTTTCTGTCTGCTTTTCAACCAACATTCTTTCTTTGGGCAAGAAAAACTCCGACCGCCTCCTCTCCTCCGCCGTCTTTCCCCTCAAGGGAAACGTTTACCCACTTGGGTTTGGATTCGATCCTTTATTTTCTTTCCACAGCTTCCAACTTTGCCTTGATTTTATTTTATGTGTGTTTTTTTCCCGCTTCATCATTGATTCTTGTTTTCTTGTTTTCTTGATTTTGTTTCTTGTATCAATTCCCCATCATTCTTGAATTTTGTTCTACTTGCCGCTGGATTTTCCTTTTTAATTAGATGCTGCTGTTCGATTCTTGTCCAATTTGATTTCGTGAAGTTCTCTTATTTTAAGTCCTTGAGTTTTTTGGTTGCTCGTTTTCTGTTATTGAGTTTTGTTTCTGACCTTTCTCTGTAGTCTATGTTCATTCAATGATTCTAACATTTCTTTTCTGTCTTTTTTGTTCATGAATCCTTTAGTTAATGGGTCGGTTAAGTTAACATATATAATACACTGTTTGGCAATCTACTTTCCATCCATTTTGAAATTTGAATAATCTTGCTACTGGGAAGTTTTTCAATAGGATGTTTCTTATCAGCTGGTTAAAATGTTTCTGAATTCAGTTTTTCCATGAACTCTCTGTTGTTGTTTGGTTGTCTGCATATTTATTTGTTTACTGTATTTGTGTTAATTCGTTGTAAATTCTTTTCTGTATCTGCAGTTACTACTCTGTGTCTATTAACATAGGCAAACGAGATGAGGCTTTTGAATTTGATATCGACTCTGGCAGTGATCTCACTTGGGTTCAATGTGATGCCCCTTGTACAGGCTGCACAAAGGTTAGTGGATCGGATGATAACAATAACCATTTTGATGTTTGCTCTCTTCGCTCTAATGTTCTTATAAATTTCACATGTATCTGCAGCCTCGTGAACAGTTATATAAACCCGACAACAATGCTTTGAACTGTTTTGAACCACTGTGTACATCACTCCACCCGATAGCCGACCACCAATGCGAGTCTGCTGATGACCATTGTCAGTATGAAATTGAGTATGCTGACCAAGGATCGTCTCTGGGTGTGTTGGTTAATGACAACGTTCCCTTGAAACTCACTAATGGTTCTCTTGCTGCTCCTCGTATAACGTTTGGGTGAGTTGTATGGATATATATCTCATGATACCGTTATCATTCATATAATCCTAACTAATCGTGGCTGCAATCCCCAGAGGCTCCATTTTGCTTTTTAAGTAAATAGTTATGAAACCTGGCTTTGTCCAAGGACATATATAAACCATATAGTGAAATTGATTTAGCTTCTATCATATTAAATTGTGAGGATATATCTGTTAGGCCCCCAATTATATACCAATGTGTACGTAGGGGTAAAAAGGAAAAAGAATCCGTTGTTAGTGGGATGGGAGAATCATCCAATAGTAAGGATGTGGGGACCATAGTTAGTTAGAGAGAGGGGAGTCGTTTATATATTGAGGGAAGGCTGAGGAAGAGGGTAGGAAAGTTTTATTAAGGAGTGGGTTTCTCTCGAGAGTGGGAGGAATAGGGTTTGTAACCTGCCTTAGGGCATTTCCTTTGAATTCAATGAAGTGTTCTTGAGTTGATTCCTAGCTGATCTTTTAATCTTTTGTCTCCAGATGTGGATACGATCATAAATACTCTGTTCCACACTCACCTCCTCCTACAGCTGGAGTTCTTGGCCTTGGCAATGGTGAAGTTAGCATTATCTCGCAACTGAGTAACATGGGGGTAGTGCGAAATGTTGTAGGTCACTGTCTTAGTGAAGAAGGTGGATTTTTATTTTTTGGCGATGAACTTGTCCCTTCTTCCGGAGTAACATGGACAAGTATGTCTTATGAATCCATAGGGTGAGTAGCCCTTTAATATCATGTTATCTTTTTGCATCACACGTAACTAAAGATTTGTATGATATTCAATCTCATCTCAGAAGTTACTACTCTTCCGGACCTGCAGAAGTCTATTTCGATGGAAAGGCTACTGGTATCAAGGATCTTATATTAGTTTTTGACAGCGGCAGCTCTTACACTTACTTCAACTCTCAAGCTTACAATGCCATTCTTGCTTTGGTAAAAACAGAGAAAAGGAACTGTTTTCTTATGCAATACATATTTGTGAAATTGTAATGTGAGATGCGATATTTTACCTCAGGTAAGGAATGATTTGAGCGGAAAACCATTGGAAGATGCACCGGAGGATAAATCTCTTCCAATATGCTGGAGAGGCACGCACCCTTTCAAGTCGTTGCACGATGTTAAGAAATACTTCAAGCCCTTTGCATTACGCTTTACAAAAACTAAGAATACTGAAATGCAACTGCCTCCAGAAACTTATCTCATCATAAGTGTGAGTATTCAACTCAATCTTGGATTCTTTGCAGGTTTCTGCTTTATGAATTATGTATTTAACAAAGTGTTCTCCAGAAATATGGCAATGTTTGCTTTGGGATTCTGAATGGAAGTGAAGTAGGACTAGGGGATCTCAACATAATTGGTGGTAAGTAACATCAGTGCCAAACAATTTGGAAACTGTGATTCCCCCTCCCCTCTTTGTGTAGTAATGTTTGGTAGTACTTAGTAATTTTAGCAATAAGTACTTCAATTATACACAATAGTAAAATAGAAGTGCTAAAAACAAAACTTAACTTTGATGTTTGGTTCTACAATCTTAATGGCATTTGAGGGGCTGAGTTGATTATTATAGTTAGTGGTTATTATAGTTTATGAGTTATAATAGTCTATTTTGGAGTGCATACTATTTTAGCATGGGTTACAATAGTTTGTGTTTTGCGTGTAGACTATTTTAGTTTGGATAGGAAATAAGTAAACACTATAACAAAGAGGGAAGGAGAGGATGAATAAAAAATGGTAAACATTGTAACAAAGAGAAATTTGAAATAATGATATTGTAGTTAATGGTAGGTTATAACCGTTTGAAACACTTACTATCAAACATGAAGTGAGATATAATAGCTCGCTCCCATCCACTTGAAGTTGGGGTCCAAGCAACTCCTTGATTATAGTCATTTAGATCAAATGCACTTTACAACTACTTTCCCATAAAGCACTTTTTACTTTTTTATGAGAGTACTAATAAATTACCTTCAAACTTGATGATTTCCTAGTTCAGAATTAGCTGACCTTGTTTCTCATTCAGACATCTCTCTAAAAGATAAATTGGTGATCTACGACAACGAGAGGCAACGAATCGGATGGTTTCCTACCAAGTGCGACAAATTTAGAAAGTAGACTACTATTCCCATCGCCTTCTTTGCTTCGATGCTCCCATTACTCTATGGATAACCTTCTCACAAGCCTTTTCTTGTTGCCCCTTTATTTTCAGGGAAGGTCAAAGTCTTTGTCGACCAGAAGGTATATTCAGTATCCTATCAGAGAATTATCAAGGCTACATCCCAAAGGTCTTTTAG

mRNA sequence

ATGCCAACAATCACAACTCTTATCTTCTTTCTTTTAGGTCTTTCTTCAATTTTTTCTGTCTGCTTTTCAACCAACATTCTTTCTTTGGGCAAGAAAAACTCCGACCGCCTCCTCTCCTCCGCCGTCTTTCCCCTCAAGGGAAACGTTTACCCACTTGGTTACTACTCTGTGTCTATTAACATAGGCAAACGAGATGAGGCTTTTGAATTTGATATCGACTCTGGCAGTGATCTCACTTGGGTTCAATGTGATGCCCCTTGTACAGGCTGCACAAAGCCTCGTGAACAGTTATATAAACCCGACAACAATGCTTTGAACTGTTTTGAACCACTGTGTACATCACTCCACCCGATAGCCGACCACCAATGCGAGTCTGCTGATGACCATTGTCAGTATGAAATTGAGTATGCTGACCAAGGATCGTCTCTGGGTGTGTTGGTTAATGACAACGTTCCCTTGAAACTCACTAATGGTTCTCTTGCTGCTCCTCGTATAACGTTTGGATGTGGATACGATCATAAATACTCTGTTCCACACTCACCTCCTCCTACAGCTGGAGTTCTTGGCCTTGGCAATGGTGAAGTTAGCATTATCTCGCAACTGAGTAACATGGGGGTAGTGCGAAATGTTGTAGGTCACTGTCTTAGTGAAGAAGGTGGATTTTTATTTTTTGGCGATGAACTTGTCCCTTCTTCCGGAGTAACATGGACAAGTATGTCTTATGAATCCATAGGAAGTTACTACTCTTCCGGACCTGCAGAAGTCTATTTCGATGGAAAGGCTACTGGTATCAAGGATCTTATATTAGTTTTTGACAGCGGCAGCTCTTACACTTACTTCAACTCTCAAGCTTACAATGCCATTCTTGCTTTGGTAAGGAATGATTTGAGCGGAAAACCATTGGAAGATGCACCGGAGGATAAATCTCTTCCAATATGCTGGAGAGGCACGCACCCTTTCAAGTCGTTGCACGATGTTAAGAAATACTTCAAGCCCTTTGCATTACGCTTTACAAAAACTAAGAATACTGAAATGCAACTGCCTCCAGAAACTTATCTCATCATAAGTAAATATGGCAATGTTTGCTTTGGGATTCTGAATGGAAGTGAAGTAGGACTAGGGGATCTCAACATAATTGGTGACATCTCTCTAAAAGATAAATTGGTGATCTACGACAACGAGAGGCAACGAATCGGATGGTTTCCTACCAAGTGCGACAAATTTAGAAAGGAAGGTCAAAGTCTTTGTCGACCAGAAGGTATATTCAGTATCCTATCAGAGAATTATCAAGGCTACATCCCAAAGGTCTTTTAG

Coding sequence (CDS)

ATGCCAACAATCACAACTCTTATCTTCTTTCTTTTAGGTCTTTCTTCAATTTTTTCTGTCTGCTTTTCAACCAACATTCTTTCTTTGGGCAAGAAAAACTCCGACCGCCTCCTCTCCTCCGCCGTCTTTCCCCTCAAGGGAAACGTTTACCCACTTGGTTACTACTCTGTGTCTATTAACATAGGCAAACGAGATGAGGCTTTTGAATTTGATATCGACTCTGGCAGTGATCTCACTTGGGTTCAATGTGATGCCCCTTGTACAGGCTGCACAAAGCCTCGTGAACAGTTATATAAACCCGACAACAATGCTTTGAACTGTTTTGAACCACTGTGTACATCACTCCACCCGATAGCCGACCACCAATGCGAGTCTGCTGATGACCATTGTCAGTATGAAATTGAGTATGCTGACCAAGGATCGTCTCTGGGTGTGTTGGTTAATGACAACGTTCCCTTGAAACTCACTAATGGTTCTCTTGCTGCTCCTCGTATAACGTTTGGATGTGGATACGATCATAAATACTCTGTTCCACACTCACCTCCTCCTACAGCTGGAGTTCTTGGCCTTGGCAATGGTGAAGTTAGCATTATCTCGCAACTGAGTAACATGGGGGTAGTGCGAAATGTTGTAGGTCACTGTCTTAGTGAAGAAGGTGGATTTTTATTTTTTGGCGATGAACTTGTCCCTTCTTCCGGAGTAACATGGACAAGTATGTCTTATGAATCCATAGGAAGTTACTACTCTTCCGGACCTGCAGAAGTCTATTTCGATGGAAAGGCTACTGGTATCAAGGATCTTATATTAGTTTTTGACAGCGGCAGCTCTTACACTTACTTCAACTCTCAAGCTTACAATGCCATTCTTGCTTTGGTAAGGAATGATTTGAGCGGAAAACCATTGGAAGATGCACCGGAGGATAAATCTCTTCCAATATGCTGGAGAGGCACGCACCCTTTCAAGTCGTTGCACGATGTTAAGAAATACTTCAAGCCCTTTGCATTACGCTTTACAAAAACTAAGAATACTGAAATGCAACTGCCTCCAGAAACTTATCTCATCATAAGTAAATATGGCAATGTTTGCTTTGGGATTCTGAATGGAAGTGAAGTAGGACTAGGGGATCTCAACATAATTGGTGACATCTCTCTAAAAGATAAATTGGTGATCTACGACAACGAGAGGCAACGAATCGGATGGTTTCCTACCAAGTGCGACAAATTTAGAAAGGAAGGTCAAAGTCTTTGTCGACCAGAAGGTATATTCAGTATCCTATCAGAGAATTATCAAGGCTACATCCCAAAGGTCTTTTAG

Protein sequence

MPTITTLIFFLLGLSSIFSVCFSTNILSLGKKNSDRLLSSAVFPLKGNVYPLGYYSVSINIGKRDEAFEFDIDSGSDLTWVQCDAPCTGCTKPREQLYKPDNNALNCFEPLCTSLHPIADHQCESADDHCQYEIEYADQGSSLGVLVNDNVPLKLTNGSLAAPRITFGCGYDHKYSVPHSPPPTAGVLGLGNGEVSIISQLSNMGVVRNVVGHCLSEEGGFLFFGDELVPSSGVTWTSMSYESIGSYYSSGPAEVYFDGKATGIKDLILVFDSGSSYTYFNSQAYNAILALVRNDLSGKPLEDAPEDKSLPICWRGTHPFKSLHDVKKYFKPFALRFTKTKNTEMQLPPETYLIISKYGNVCFGILNGSEVGLGDLNIIGDISLKDKLVIYDNERQRIGWFPTKCDKFRKEGQSLCRPEGIFSILSENYQGYIPKVF
Homology
BLAST of Cla97C08G148580 vs. NCBI nr
Match: XP_038885447.1 (aspartic proteinase Asp1-like [Benincasa hispida])

HSP 1 Score: 823.9 bits (2127), Expect = 6.4e-235
Identity = 390/437 (89.24%), Postives = 416/437 (95.19%), Query Frame = 0

Query: 1   MPTITTLIFFLLGLSSIFSVCFSTNILSLGKKNSDRLLSSAVFPLKGNVYPLGYYSVSIN 60
           MPTITTL FFLLGLSS FSVCFSTNILSLGKKNSDRLLSSAVFPLKGNVYPLGYYSVSI+
Sbjct: 24  MPTITTLFFFLLGLSSSFSVCFSTNILSLGKKNSDRLLSSAVFPLKGNVYPLGYYSVSIH 83

Query: 61  IGKRDEAFEFDIDSGSDLTWVQCDAPCTGCTKPREQLYKPDNNALNCFEPLCTSLHPIAD 120
           IG+ DEAFEFDIDSGSDLTWVQCDAPCTGCTKPRE+LYKP+NNALNCFEPLCTSLHPI +
Sbjct: 84  IGEGDEAFEFDIDSGSDLTWVQCDAPCTGCTKPRERLYKPNNNALNCFEPLCTSLHPITN 143

Query: 121 HQCESADDHCQYEIEYADQGSSLGVLVNDNVPLKLTNGSLAAPRITFGCGYDHKYSVPHS 180
            QCESADD CQYEIEYAD GSSLGVLVND+VPLKLTNGS AAPR+ FGCGYD+KYSVPHS
Sbjct: 144 DQCESADDQCQYEIEYADHGSSLGVLVNDHVPLKLTNGSTAAPRVAFGCGYDYKYSVPHS 203

Query: 181 PPPTAGVLGLGNGEVSIISQLSNMGVVRNVVGHCLSEEGGFLFFGDELVPSSGVTWTSMS 240
           PPPTAGVLGLGNGEVSIISQLSNMGVVRNVVGHCLSEEGGFLFFGDELVPSSGV WTSMS
Sbjct: 204 PPPTAGVLGLGNGEVSIISQLSNMGVVRNVVGHCLSEEGGFLFFGDELVPSSGVAWTSMS 263

Query: 241 YESIGSYYSSGPAEVYFDGKATGIKDLILVFDSGSSYTYFNSQAYNAILALVRNDLSGKP 300
           +ESIGSYYSSGPAEV++ GKATGIKDLILVFDSGSSYTYFNSQAYN+ILALVRNDL GKP
Sbjct: 264 HESIGSYYSSGPAEVHYGGKATGIKDLILVFDSGSSYTYFNSQAYNSILALVRNDLKGKP 323

Query: 301 LEDAPEDKSLPICWRGTHPFKSLHDVKKYFKPFALRFTKTKNTEMQLPPETYLIISKYGN 360
           LEDAPEDKSLPICW+G  PFKSL DVKKYFKP AL FTKTKN ++QLP ETYLII+KYGN
Sbjct: 324 LEDAPEDKSLPICWKGKRPFKSLRDVKKYFKPLALCFTKTKNAQLQLPLETYLIITKYGN 383

Query: 361 VCFGILNGSEVGLGDLNIIGDISLKDKLVIYDNERQRIGWFPTKCDKFRKEGQSLCRPEG 420
           VCFGILNG+EVGLGDLNIIGDISLKDK+VIYDNER+RIGWFPT C+KF+KEGQS C+P+G
Sbjct: 384 VCFGILNGTEVGLGDLNIIGDISLKDKMVIYDNERRRIGWFPTNCNKFKKEGQSFCQPDG 443

Query: 421 IFSILSENYQGYIPKVF 438
           +FSIL+ENYQGYIPK+F
Sbjct: 444 LFSILTENYQGYIPKIF 460

BLAST of Cla97C08G148580 vs. NCBI nr
Match: XP_004149806.1 (aspartic proteinase Asp1 [Cucumis sativus] >XP_011657431.1 aspartic proteinase Asp1 [Cucumis sativus] >KGN47712.1 hypothetical protein Csa_018238 [Cucumis sativus])

HSP 1 Score: 811.2 bits (2094), Expect = 4.3e-231
Identity = 385/437 (88.10%), Postives = 409/437 (93.59%), Query Frame = 0

Query: 1   MPTITTLIFFLLGLSSIFSVCFSTNILSLGKKNSDRLLSSAVFPLKGNVYPLGYYSVSIN 60
           M TIT+L FFLLGL SIF V FSTNILSL KKNSDRLLSS VFPLKGNVYPLGYYSVSIN
Sbjct: 1   MTTITSLFFFLLGLFSIFPVSFSTNILSLRKKNSDRLLSSVVFPLKGNVYPLGYYSVSIN 60

Query: 61  IGKRDEAFEFDIDSGSDLTWVQCDAPCTGCTKPREQLYKPDNNALNCFEPLCTSLHPIAD 120
           IGK DEAFEFDIDSGSDLTWVQCDAPCT CTKPREQLYKP+NNALNCFEPLCTSLHPI +
Sbjct: 61  IGKGDEAFEFDIDSGSDLTWVQCDAPCTHCTKPREQLYKPNNNALNCFEPLCTSLHPITN 120

Query: 121 HQCESADDHCQYEIEYADQGSSLGVLVNDNVPLKLTNGSLAAPRITFGCGYDHKYSVPHS 180
           H C+SADD CQYEIEYAD GSSLGVLVND+VPLKLTNGSLAAPRI FGCGYDHKYSVP S
Sbjct: 121 HHCKSADDQCQYEIEYADHGSSLGVLVNDHVPLKLTNGSLAAPRIAFGCGYDHKYSVPDS 180

Query: 181 PPPTAGVLGLGNGEVSIISQLSNMGVVRNVVGHCLSEEGGFLFFGDELVPSSGVTWTSMS 240
            PPTAGVLGLGNGEVS ISQLS+MGVVRNVVGHCLS+EGGFLFFGDE VPSSGVTWTSMS
Sbjct: 181 SPPTAGVLGLGNGEVSFISQLSSMGVVRNVVGHCLSDEGGFLFFGDEFVPSSGVTWTSMS 240

Query: 241 YESIGSYYSSGPAEVYFDGKATGIKDLILVFDSGSSYTYFNSQAYNAILALVRNDLSGKP 300
           +ESIGSYYSSGPAEVYF GKATGIKDL LVFDSGSSYTYFNSQAYN+ILALV+N+L GKP
Sbjct: 241 HESIGSYYSSGPAEVYFSGKATGIKDLTLVFDSGSSYTYFNSQAYNSILALVKNNLRGKP 300

Query: 301 LEDAPEDKSLPICWRGTHPFKSLHDVKKYFKPFALRFTKTKNTEMQLPPETYLIISKYGN 360
           LEDAPEDKSLP+CW+GT PFKSL DVKKYF P ALRFTKTKN ++QLPPE YLII+KYGN
Sbjct: 301 LEDAPEDKSLPVCWKGTRPFKSLRDVKKYFNPLALRFTKTKNAQIQLPPENYLIITKYGN 360

Query: 361 VCFGILNGSEVGLGDLNIIGDISLKDKLVIYDNERQRIGWFPTKCDKFRKEGQSLCRPEG 420
           VCFGILNG+EVGLGDLNIIGDISLKDK+VIYDNER+RIGWFPT C+KFRKEGQSLC+PEG
Sbjct: 361 VCFGILNGTEVGLGDLNIIGDISLKDKMVIYDNERRRIGWFPTNCNKFRKEGQSLCQPEG 420

Query: 421 IFSILSENYQGYIPKVF 438
           +FSIL+ENYQGYIPK+F
Sbjct: 421 LFSILTENYQGYIPKIF 437

BLAST of Cla97C08G148580 vs. NCBI nr
Match: XP_008466731.1 (PREDICTED: aspartic proteinase Asp1 [Cucumis melo] >KAA0055449.1 aspartic proteinase Asp1 [Cucumis melo var. makuwa] >TYK08931.1 aspartic proteinase Asp1 [Cucumis melo var. makuwa])

HSP 1 Score: 804.3 bits (2076), Expect = 5.2e-229
Identity = 381/437 (87.19%), Postives = 406/437 (92.91%), Query Frame = 0

Query: 1   MPTITTLIFFLLGLSSIFSVCFSTNILSLGKKNSDRLLSSAVFPLKGNVYPLGYYSVSIN 60
           M TIT+  FFLLGLSSIF V FSTNILSLGKKNSDRLLSS VFPLKGNVYPLGYYSVSIN
Sbjct: 1   MTTITSFFFFLLGLSSIFPVSFSTNILSLGKKNSDRLLSSVVFPLKGNVYPLGYYSVSIN 60

Query: 61  IGKRDEAFEFDIDSGSDLTWVQCDAPCTGCTKPREQLYKPDNNALNCFEPLCTSLHPIAD 120
           IGK DEAFEFDIDSGSDLTWVQCDAPCT CTKPREQLYKP+NNALNCFEPLC SLHPI +
Sbjct: 61  IGKGDEAFEFDIDSGSDLTWVQCDAPCTDCTKPREQLYKPNNNALNCFEPLCISLHPITN 120

Query: 121 HQCESADDHCQYEIEYADQGSSLGVLVNDNVPLKLTNGSLAAPRITFGCGYDHKYSVPHS 180
           H C+SADD CQYEIEYAD GSSLGVLVND+VPLKLTNGSLA PRI FGCGY+HKYSVP S
Sbjct: 121 HHCKSADDQCQYEIEYADHGSSLGVLVNDHVPLKLTNGSLATPRIAFGCGYEHKYSVPDS 180

Query: 181 PPPTAGVLGLGNGEVSIISQLSNMGVVRNVVGHCLSEEGGFLFFGDELVPSSGVTWTSMS 240
           PPPTAGVLGLGNGEVS ISQLSNMGVVRNVVGHCLSEEGGFLFFGDELVPSSGVTWTSMS
Sbjct: 181 PPPTAGVLGLGNGEVSFISQLSNMGVVRNVVGHCLSEEGGFLFFGDELVPSSGVTWTSMS 240

Query: 241 YESIGSYYSSGPAEVYFDGKATGIKDLILVFDSGSSYTYFNSQAYNAILALVRNDLSGKP 300
           +ESIG YYSSGPAEVYF GKATGIKDL LVFDSGSSYTYFNSQAYN+ILALVRN+L GKP
Sbjct: 241 HESIGGYYSSGPAEVYFGGKATGIKDLTLVFDSGSSYTYFNSQAYNSILALVRNNLRGKP 300

Query: 301 LEDAPEDKSLPICWRGTHPFKSLHDVKKYFKPFALRFTKTKNTEMQLPPETYLIISKYGN 360
           LEDAPEDKSLP+CW+G  PFKSLHDVKKYF P ALRFTKTK  ++QL PE YLII+KYGN
Sbjct: 301 LEDAPEDKSLPVCWKGPRPFKSLHDVKKYFNPLALRFTKTKKAQIQLAPEAYLIITKYGN 360

Query: 361 VCFGILNGSEVGLGDLNIIGDISLKDKLVIYDNERQRIGWFPTKCDKFRKEGQSLCRPEG 420
           VCFGILNG+EVGLG+LNIIGDISLKDK+VIYDNER++IGWFPT C+KF+KEGQSLC+PEG
Sbjct: 361 VCFGILNGTEVGLGNLNIIGDISLKDKMVIYDNERRQIGWFPTNCNKFKKEGQSLCQPEG 420

Query: 421 IFSILSENYQGYIPKVF 438
           +FSIL+ENYQGYI K+F
Sbjct: 421 LFSILTENYQGYIRKIF 437

BLAST of Cla97C08G148580 vs. NCBI nr
Match: KAG6602107.1 (Aspartyl protease APCB1, partial [Cucurbita argyrosperma subsp. sororia])

HSP 1 Score: 763.1 bits (1969), Expect = 1.3e-216
Identity = 361/437 (82.61%), Postives = 390/437 (89.24%), Query Frame = 0

Query: 1   MPTITTLIFFLLGLSSIFSVCFSTNILSLGKKNSDRLLSSAVFPLKGNVYPLGYYSVSIN 60
           MP   TLI FLL L + FSVCFS NI SL KKNSDRLLSSAVFPLKGNVYPLGYYSVSIN
Sbjct: 1   MPRTPTLILFLLPLLATFSVCFSANIFSLRKKNSDRLLSSAVFPLKGNVYPLGYYSVSIN 60

Query: 61  IGKRDEAFEFDIDSGSDLTWVQCDAPCTGCTKPREQLYKPDNNALNCFEPLCTSLHPIAD 120
           IGK DEAFEFDIDSGSDLTWVQCDAPCTGCTKPR QLYKPDNNAL CFEPLC SLH  AD
Sbjct: 61  IGKGDEAFEFDIDSGSDLTWVQCDAPCTGCTKPRGQLYKPDNNALTCFEPLCASLHLTAD 120

Query: 121 HQCESADDHCQYEIEYADQGSSLGVLVNDNVPLKLTNGSLAAPRITFGCGYDHKYSVPHS 180
            QCESAD+ CQYEIEYAD GSSLGVLVND+ PLKLTNGSLAAPRI FGCGYDHKYSVP+ 
Sbjct: 121 QQCESADEQCQYEIEYADHGSSLGVLVNDHFPLKLTNGSLAAPRIAFGCGYDHKYSVPNP 180

Query: 181 PPPTAGVLGLGNGEVSIISQLSNMGVVRNVVGHCLSEEGGFLFFGDELVPSSGVTWTSMS 240
           PPPTAGVLGLGNGEVSIISQLSNMG++RNVVGHCLSE+GGFLFFGDEL+PSSGV WTSMS
Sbjct: 181 PPPTAGVLGLGNGEVSIISQLSNMGIMRNVVGHCLSEQGGFLFFGDELIPSSGVAWTSMS 240

Query: 241 YESIGSYYSSGPAEVYFDGKATGIKDLILVFDSGSSYTYFNSQAYNAILALVRNDLSGKP 300
            ESIG++YSSGPAEVYF GKA GIK L LVFDSGSSYTYF SQ Y+++LALVR+DL GKP
Sbjct: 241 SESIGNHYSSGPAEVYFGGKAAGIKGLTLVFDSGSSYTYFTSQVYDSVLALVRDDLKGKP 300

Query: 301 LEDAPEDKSLPICWRGTHPFKSLHDVKKYFKPFALRFTKTKNTEMQLPPETYLIISKYGN 360
           LEDAPEDKSLPICWRG  PFKSL DVK YF+P AL FTKTKN ++QLPPE+YLI++KYGN
Sbjct: 301 LEDAPEDKSLPICWRGPQPFKSLRDVKNYFRPLALHFTKTKNAQIQLPPESYLIVTKYGN 360

Query: 361 VCFGILNGSEVGLGDLNIIGDISLKDKLVIYDNERQRIGWFPTKCDKFRKEGQSLCRPEG 420
           VCFGILNG+EVGLGDLNIIGD+SL+DK+VIYDNER++IGWF T C+KF+KEGQS C PEG
Sbjct: 361 VCFGILNGTEVGLGDLNIIGDVSLQDKMVIYDNERRKIGWFSTSCNKFQKEGQSFCEPEG 420

Query: 421 IFSILSENYQGYIPKVF 438
             SILS NY  YIPK+F
Sbjct: 421 PLSILSGNYYEYIPKIF 437

BLAST of Cla97C08G148580 vs. NCBI nr
Match: XP_023524501.1 (aspartic proteinase Asp1-like [Cucurbita pepo subsp. pepo])

HSP 1 Score: 761.9 bits (1966), Expect = 3.0e-216
Identity = 361/437 (82.61%), Postives = 390/437 (89.24%), Query Frame = 0

Query: 1   MPTITTLIFFLLGLSSIFSVCFSTNILSLGKKNSDRLLSSAVFPLKGNVYPLGYYSVSIN 60
           MP   TLI FLL L + FSVCFS NI SL KKNSDRLLSSAVFPLKGNVYPLGYYSVSIN
Sbjct: 1   MPRTPTLILFLLLLLATFSVCFSANIFSLRKKNSDRLLSSAVFPLKGNVYPLGYYSVSIN 60

Query: 61  IGKRDEAFEFDIDSGSDLTWVQCDAPCTGCTKPREQLYKPDNNALNCFEPLCTSLHPIAD 120
           IGK DEAFEFDIDSGSDLTWVQCDAPCTGCTKPR QLYKPDNNAL CFEPLC SLH  AD
Sbjct: 61  IGKGDEAFEFDIDSGSDLTWVQCDAPCTGCTKPRGQLYKPDNNALTCFEPLCASLHLTAD 120

Query: 121 HQCESADDHCQYEIEYADQGSSLGVLVNDNVPLKLTNGSLAAPRITFGCGYDHKYSVPHS 180
            QCESAD+ CQYEIEYAD GSSLGVLVND+ PLKLTNGSLAAPRI FGCGYDHKYSVP+ 
Sbjct: 121 QQCESADEQCQYEIEYADHGSSLGVLVNDHFPLKLTNGSLAAPRIAFGCGYDHKYSVPNP 180

Query: 181 PPPTAGVLGLGNGEVSIISQLSNMGVVRNVVGHCLSEEGGFLFFGDELVPSSGVTWTSMS 240
           PPPTAGVLGLGNGEVSIISQLSNMG++RNVVGHCLSE+GGFLFFGDEL+PSSGV WTSMS
Sbjct: 181 PPPTAGVLGLGNGEVSIISQLSNMGIMRNVVGHCLSEQGGFLFFGDELIPSSGVAWTSMS 240

Query: 241 YESIGSYYSSGPAEVYFDGKATGIKDLILVFDSGSSYTYFNSQAYNAILALVRNDLSGKP 300
            ESIG++YSSGPAEVYF GKA GIK L LVFDSGSSYTYF SQ Y+++LALVR+DL GKP
Sbjct: 241 SESIGNHYSSGPAEVYFGGKAAGIKGLTLVFDSGSSYTYFTSQVYDSVLALVRDDLRGKP 300

Query: 301 LEDAPEDKSLPICWRGTHPFKSLHDVKKYFKPFALRFTKTKNTEMQLPPETYLIISKYGN 360
           LEDAPEDKSLPICWRG  PFKSL DVK YF+P AL FTKTKN ++QLPPE+YLI++KYGN
Sbjct: 301 LEDAPEDKSLPICWRGPQPFKSLRDVKNYFRPLALHFTKTKNAQIQLPPESYLIVTKYGN 360

Query: 361 VCFGILNGSEVGLGDLNIIGDISLKDKLVIYDNERQRIGWFPTKCDKFRKEGQSLCRPEG 420
           VCFGILNG+EVGLGDLNIIGD+SL+DK+VIYDNER++IGWF T C+KF+KEGQS C PEG
Sbjct: 361 VCFGILNGTEVGLGDLNIIGDVSLQDKMVIYDNERRKIGWFSTSCNKFQKEGQSFCEPEG 420

Query: 421 IFSILSENYQGYIPKVF 438
             SILS NY  YIPK+F
Sbjct: 421 PLSILSGNYYEYIPKIF 437

BLAST of Cla97C08G148580 vs. ExPASy Swiss-Prot
Match: Q9M9A8 (Aspartyl protease APCB1 OS=Arabidopsis thaliana OX=3702 GN=APCB1 PE=1 SV=1)

HSP 1 Score: 315.1 bits (806), Expect = 1.3e-84
Identity = 169/392 (43.11%), Postives = 241/392 (61.48%), Query Frame = 0

Query: 39  SSAVFPLKGNVYPLGYYSVSINIGKRDEA--FEFDIDSGSDLTWVQCDAPCTGCTKPREQ 98
           S+ +FP+ GNVYP G Y   I +GK ++   +  DID+GS+LTW+QCDAPCT C K   Q
Sbjct: 187 STTIFPVGGNVYPDGLYYTRILVGKPEDGQYYHLDIDTGSELTWIQCDAPCTSCAKGANQ 246

Query: 99  LYKP-DNNALNCFEPLCTSL--HPIADHQCESADDHCQYEIEYADQGSSLGVLVNDNVPL 158
           LYKP  +N +   E  C  +  + + +H CE+    C YEIEYAD   S+GVL  D   L
Sbjct: 247 LYKPRKDNLVRSSEAFCVEVQRNQLTEH-CENC-HQCDYEIEYADHSYSMGVLTKDKFHL 306

Query: 159 KLTNGSLAAPRITFGCGYDHKYSVPHSPPPTAGVLGLGNGEVSIISQLSNMGVVRNVVGH 218
           KL NGSLA   I FGCGYD +  + ++   T G+LGL   ++S+ SQL++ G++ NVVGH
Sbjct: 307 KLHNGSLAESDIVFGCGYDQQGLLLNTLLKTDGILGLSRAKISLPSQLASRGIISNVVGH 366

Query: 219 CLSEE---GGFLFFGDELVPSSGVTWTSMSYESIGSYY-------SSGPAEVYFDGKATG 278
           CL+ +    G++F G +LVPS G+TW  M ++S    Y       S G   +  DG+   
Sbjct: 367 CLASDLNGEGYIFMGSDLVPSHGMTWVPMLHDSRLDAYQMQVTKMSYGQGMLSLDGENGR 426

Query: 279 IKDLILVFDSGSSYTYFNSQAYNAILALVRNDLSGKPLEDAPEDKSLPICWRG--THPFK 338
           +  ++  FD+GSSYTYF +QAY+ ++  ++ ++SG  L     D++LPICWR     PF 
Sbjct: 427 VGKVL--FDTGSSYTYFPNQAYSQLVTSLQ-EVSGLELTRDDSDETLPICWRAKTNFPFS 486

Query: 339 SLHDVKKYFKPFALRFTK---TKNTEMQLPPETYLIISKYGNVCFGILNGSEVGLGDLNI 398
           SL DVKK+F+P  L+        + ++ + PE YLIIS  GNVC GIL+GS V  G   I
Sbjct: 487 SLSDVKKFFRPITLQIGSKWLIISRKLLIQPEDYLIISNKGNVCLGILDGSSVHDGSTII 546

Query: 399 IGDISLKDKLVIYDNERQRIGWFPTKCDKFRK 411
           +GDIS++  L++YDN ++RIGW  + C + R+
Sbjct: 547 LGDISMRGHLIVYDNVKRRIGWMKSDCVRPRE 573

BLAST of Cla97C08G148580 vs. ExPASy Swiss-Prot
Match: Q0IU52 (Aspartic proteinase Asp1 OS=Oryza sativa subsp. japonica OX=39947 GN=ASP1 PE=2 SV=1)

HSP 1 Score: 308.5 bits (789), Expect = 1.2e-82
Identity = 162/389 (41.65%), Postives = 242/389 (62.21%), Query Frame = 0

Query: 39  SSAVFPLKGNVYPLGYYSVSINIGKRDEAFEFDIDSGSDLTWVQCDAPCTGCTKPREQLY 98
           S+ V  L GNVYP+G++ +++NIG   +++  DID+GS LTW+QCDAPCT C      LY
Sbjct: 22  SAVVLELHGNVYPIGHFFITMNIGDPAKSYFLDIDTGSTLTWLQCDAPCTNCNIVPHVLY 81

Query: 99  KPDNNAL-NCFEPLCTSLH-PIADHQCESADDHCQYEIEYADQGSSLGVLVNDNVPLKLT 158
           KP    L  C + LCT L+  +   +   +   C Y I+Y D  SS+GVLV D   L  +
Sbjct: 82  KPTPKKLVTCADSLCTDLYTDLGKPKRCGSQKQCDYVIQYVD-SSSMGVLVIDRFSLSAS 141

Query: 159 NGSLAAPRITFGCGYDHKYSVPHSPPPTAGVLGLGNGEVSIISQLSNMGVV-RNVVGHCL 218
           NG+     I FGCGYD      + P P   +LGL  G+V+++SQL + GV+ ++V+GHC+
Sbjct: 142 NGT-NPTTIAFGCGYDQGKKNRNVPIPVDSILGLSRGKVTLLSQLKSQGVITKHVLGHCI 201

Query: 219 SEE-GGFLFFGDELVPSSGVTWTSMSYESIGSYYSSGPAEVYFD--GKATGIKDLILVFD 278
           S + GGFLFFGD  VP+SGVTWT M+ E    YYS G   ++FD   KA     + ++FD
Sbjct: 202 SSKGGGFLFFGDAQVPTSGVTWTPMNREH--KYYSPGHGTLHFDSNSKAISAAPMAVIFD 261

Query: 279 SGSSYTYFNSQAYNAILALVRNDLSGK---PLEDAPEDKSLPICWRGTHPFKSLHDVKKY 338
           SG++YTYF +Q Y A L++V++ L+ +     E   +D++L +CW+G     ++ +VKK 
Sbjct: 262 SGATYTYFAAQPYQATLSVVKSTLNSECKFLTEVTEKDRALTVCWKGKDKIVTIDEVKKC 321

Query: 339 FKPFALRFTK-TKNTEMQLPPETYLIISKYGNVCFGILNGSE--VGLGDLNIIGDISLKD 398
           F+  +L F    K   +++PPE YLIIS+ G+VC GIL+GS+  + L   N+IG I++ D
Sbjct: 322 FRSLSLEFADGDKKATLEIPPEHYLIISQEGHVCLGILDGSKEHLSLAGTNLIGGITMLD 381

Query: 399 KLVIYDNERQRIGWFPTKCDKFRKEGQSL 416
           ++VIYD+ER  +GW   +CD+  +   ++
Sbjct: 382 QMVIYDSERSLLGWVNYQCDRIPRSESAI 406

BLAST of Cla97C08G148580 vs. ExPASy Swiss-Prot
Match: A2ZC67 (Aspartic proteinase Asp1 OS=Oryza sativa subsp. indica OX=39946 GN=ASP1 PE=2 SV=2)

HSP 1 Score: 297.7 bits (761), Expect = 2.1e-79
Identity = 159/389 (40.87%), Postives = 243/389 (62.47%), Query Frame = 0

Query: 39  SSAVFPLKGNVYPLGYYSVSINIGKRDEAFEFDIDSGSDLTWVQCDAPCTGCTKPREQLY 98
           S+ V  L GNVYP+G++ V++NIG   + +  DID+GS LTW+QCD PC  C K    LY
Sbjct: 22  SAVVLELHGNVYPIGHFFVTMNIGDPAKPYFLDIDTGSTLTWLQCDYPCINCNKVPHGLY 81

Query: 99  KPD-NNALNCFEPLCTSLHPIADHQCE-SADDHCQYEIEYADQGSSLGVLVNDNVPLKLT 158
           KP+   A+ C E  C  L+       +    + C Y I+Y   GSS+GVL+ D+  L  +
Sbjct: 82  KPELKYAVKCTEQRCADLYADLRKPMKCGPKNQCHYGIQYVG-GSSIGVLIVDSFSLPAS 141

Query: 159 NGSLAAPRITFGCGYDHKYSVPHSPPPTAGVLGLGNGEVSIISQLSNMGVV-RNVVGHCL 218
           NG+     I FGCGY+   +  + P P  G+LGLG G+V+++SQL + GV+ ++V+GHC+
Sbjct: 142 NGT-NPTSIAFGCGYNQGKNNHNVPTPVNGILGLGRGKVTLLSQLKSQGVITKHVLGHCI 201

Query: 219 SEEG-GFLFFGDELVPSSGVTWTSMSYESIGSYYSSGPAEVYFDGKATGIK--DLILVFD 278
           S +G GFLFFGD  VP+SGVTW+ M+ E    +YS     + F+  +  I    + ++FD
Sbjct: 202 SSKGKGFLFFGDAKVPTSGVTWSPMNREH--KHYSPRQGTLQFNSNSKPISAAPMEVIFD 261

Query: 279 SGSSYTYFNSQAYNAILALVRNDLSG--KPLEDAPE-DKSLPICWRGTHPFKSLHDVKKY 338
           SG++YTYF  Q Y+A L++V++ LS   K L +  E D++L +CW+G    +++ +VKK 
Sbjct: 262 SGATYTYFALQPYHATLSVVKSTLSKECKFLTEVKEKDRALTVCWKGKDKIRTIDEVKKC 321

Query: 339 FKPFALRFTK-TKNTEMQLPPETYLIISKYGNVCFGILNGSE--VGLGDLNIIGDISLKD 398
           F+  +L+F    K   +++PPE YLIIS+ G+VC GIL+GS+    L   N+IG I++ D
Sbjct: 322 FRSLSLKFADGDKKATLEIPPEHYLIISQEGHVCLGILDGSKEHPSLAGTNLIGGITMLD 381

Query: 399 KLVIYDNERQRIGWFPTKCDKFRKEGQSL 416
           ++VIYD+ER  +GW   +CD+  +   ++
Sbjct: 382 QMVIYDSERSLLGWVNYQCDRIPRSASAI 406

BLAST of Cla97C08G148580 vs. ExPASy Swiss-Prot
Match: Q9S9K4 (Aspartic proteinase 39 OS=Arabidopsis thaliana OX=3702 GN=A39 PE=1 SV=2)

HSP 1 Score: 134.0 bits (336), Expect = 4.0e-30
Identity = 109/421 (25.89%), Postives = 184/421 (43.71%), Query Frame = 0

Query: 32  KNSDRLLSSAVFPLKGN--VYPLGYYSVSINIGKRDEAFEFDIDSGSDLTWVQCDAPCTG 91
           +   R+L+S   PL G+  V  +G Y   I +G   + +   +D+GSD+ W+ C  PC  
Sbjct: 49  RRHSRMLASIDLPLGGDSRVDSVGLYFTKIKLGSPPKEYHVQVDTGSDILWINC-KPCPK 108

Query: 92  C---TKPREQLYKPDNNA------LNCFEPLCTSLHPIADHQCESADDHCQYEIEYADQG 151
           C   T    +L   D NA      + C +  C+ +       C+ A   C Y I YAD+ 
Sbjct: 109 CPTKTNLNFRLSLFDMNASSTSKKVGCDDDFCSFIS--QSDSCQPALG-CSYHIVYADES 168

Query: 152 SSLGVLVNDNVPLKLTNGSL----AAPRITFGCGYDHKYSVPHSPPPTAGVLGLGNGEVS 211
           +S G  + D + L+   G L        + FGCG D    + +      GV+G G    S
Sbjct: 169 TSDGKFIRDMLTLEQVTGDLKTGPLGQEVVFGCGSDQSGQLGNGDSAVDGVMGFGQSNTS 228

Query: 212 IISQLSNMGVVRNVVGHCLSEEGGFLFFGDELVPSSGVTWTSMSYESIGSYYSSGPAEVY 271
           ++SQL+  G  + V  HCL    G   F   +V S  V  T M    +  +Y+     + 
Sbjct: 229 VLSQLAATGDAKRVFSHCLDNVKGGGIFAVGVVDSPKVKTTPMVPNQM--HYNVMLMGMD 288

Query: 272 FDGKATGIKDLIL-----VFDSGSSYTYFNSQAYNAILALVRNDLSGKPLEDAPEDKSLP 331
            DG +  +   I+     + DSG++  YF    Y++++  +   L+ +P++       L 
Sbjct: 289 VDGTSLDLPRSIVRNGGTIVDSGTTLAYFPKVLYDSLIETI---LARQPVK-------LH 348

Query: 332 ICWRGTHPFKSLHDVKKYFKPFALRFTKTKNTEMQLPPETYLIISKYGNVCFGILNG--S 391
           I       F    +V + F P +  F    + ++ + P  YL   +    CFG   G  +
Sbjct: 349 IVEETFQCFSFSTNVDEAFPPVSFEF--EDSVKLTVYPHDYLFTLEEELYCFGWQAGGLT 408

Query: 392 EVGLGDLNIIGDISLKDKLVIYDNERQRIGWFPTKCDKF--RKEGQSLCRPEGIFSILSE 429
                ++ ++GD+ L +KLV+YD + + IGW    C      K+G       G++S+ ++
Sbjct: 409 TDERSEVILLGDLVLSNKLVVYDLDNEVIGWADHNCSSSIKIKDGSG-----GVYSVGAD 446

BLAST of Cla97C08G148580 vs. ExPASy Swiss-Prot
Match: Q4V3D2 (Aspartic proteinase 36 OS=Arabidopsis thaliana OX=3702 GN=A36 PE=1 SV=1)

HSP 1 Score: 126.3 bits (316), Expect = 8.3e-28
Identity = 100/394 (25.38%), Postives = 165/394 (41.88%), Query Frame = 0

Query: 36  RLLSSAVFPLKGN--VYPLGYYSVSINIGKRDEAFEFDIDSGSDLTWVQCDAPCTGCTKP 95
           R+L++   PL G+     +G Y   I +G   + +   +D+GSD+ WV C APC  C   
Sbjct: 57  RMLANIDLPLGGDSRADSIGLYFTKIKLGSPPKEYYVQVDTGSDILWVNC-APCPKCPVK 116

Query: 96  RE-----QLYKPDNNA----LNCFEPLCTSLHPIADHQCESADDHCQYEIEYADQGSSLG 155
            +      LY    ++    + C +  C+    I   +   A   C Y + Y D  +S G
Sbjct: 117 TDLGIPLSLYDSKTSSTSKNVGCEDDFCSF---IMQSETCGAKKPCSYHVVYGDGSTSDG 176

Query: 156 VLVNDNVPLKLTNGSL----AAPRITFGCGYDHKYSVPHSPPPTAGVLGLGNGEVSIISQ 215
             + DN+ L+   G+L     A  + FGCG +    +  +     G++G G    SIISQ
Sbjct: 177 DFIKDNITLEQVTGNLRTAPLAQEVVFGCGKNQSGQLGQTDSAVDGIMGFGQSNTSIISQ 236

Query: 216 LSNMGVVRNVVGHCLSE-EGGFLFFGDE----LVPSSGVTWTSMSYESI--GSYYSSGPA 275
           L+  G  + +  HCL    GG +F   E    +V ++ +    + Y  I  G      P 
Sbjct: 237 LAAGGSTKRIFSHCLDNMNGGGIFAVGEVESPVVKTTPIVPNQVHYNVILKGMDVDGDPI 296

Query: 276 EVYFDGKATGIKDLILVFDSGSSYTYFNSQAYNAILALVRNDLSGKPLEDAPEDKSLPIC 335
           ++     A+   D   + DSG++  Y     YN+++  +           A +   L + 
Sbjct: 297 DLP-PSLASTNGDGGTIIDSGTTLAYLPQNLYNSLIEKI----------TAKQQVKLHMV 356

Query: 336 WRGTHPFKSLHDVKKYFKPFALRFTKTKNTEMQLPPETYLIISKYGNVCFGILNGSEVGL 395
                 F    +  K F    L F    + ++ + P  YL   +    CFG  +G     
Sbjct: 357 QETFACFSFTSNTDKAFPVVNLHF--EDSLKLSVYPHDYLFSLREDMYCFGWQSGGMTTQ 416

Query: 396 --GDLNIIGDISLKDKLVIYDNERQRIGWFPTKC 406
              D+ ++GD+ L +KLV+YD E + IGW    C
Sbjct: 417 DGADVILLGDLVLSNKLVVYDLENEVIGWADHNC 433

BLAST of Cla97C08G148580 vs. ExPASy TrEMBL
Match: A0A0A0KH37 (Peptidase A1 domain-containing protein OS=Cucumis sativus OX=3659 GN=Csa_6G383000 PE=3 SV=1)

HSP 1 Score: 811.2 bits (2094), Expect = 2.1e-231
Identity = 385/437 (88.10%), Postives = 409/437 (93.59%), Query Frame = 0

Query: 1   MPTITTLIFFLLGLSSIFSVCFSTNILSLGKKNSDRLLSSAVFPLKGNVYPLGYYSVSIN 60
           M TIT+L FFLLGL SIF V FSTNILSL KKNSDRLLSS VFPLKGNVYPLGYYSVSIN
Sbjct: 1   MTTITSLFFFLLGLFSIFPVSFSTNILSLRKKNSDRLLSSVVFPLKGNVYPLGYYSVSIN 60

Query: 61  IGKRDEAFEFDIDSGSDLTWVQCDAPCTGCTKPREQLYKPDNNALNCFEPLCTSLHPIAD 120
           IGK DEAFEFDIDSGSDLTWVQCDAPCT CTKPREQLYKP+NNALNCFEPLCTSLHPI +
Sbjct: 61  IGKGDEAFEFDIDSGSDLTWVQCDAPCTHCTKPREQLYKPNNNALNCFEPLCTSLHPITN 120

Query: 121 HQCESADDHCQYEIEYADQGSSLGVLVNDNVPLKLTNGSLAAPRITFGCGYDHKYSVPHS 180
           H C+SADD CQYEIEYAD GSSLGVLVND+VPLKLTNGSLAAPRI FGCGYDHKYSVP S
Sbjct: 121 HHCKSADDQCQYEIEYADHGSSLGVLVNDHVPLKLTNGSLAAPRIAFGCGYDHKYSVPDS 180

Query: 181 PPPTAGVLGLGNGEVSIISQLSNMGVVRNVVGHCLSEEGGFLFFGDELVPSSGVTWTSMS 240
            PPTAGVLGLGNGEVS ISQLS+MGVVRNVVGHCLS+EGGFLFFGDE VPSSGVTWTSMS
Sbjct: 181 SPPTAGVLGLGNGEVSFISQLSSMGVVRNVVGHCLSDEGGFLFFGDEFVPSSGVTWTSMS 240

Query: 241 YESIGSYYSSGPAEVYFDGKATGIKDLILVFDSGSSYTYFNSQAYNAILALVRNDLSGKP 300
           +ESIGSYYSSGPAEVYF GKATGIKDL LVFDSGSSYTYFNSQAYN+ILALV+N+L GKP
Sbjct: 241 HESIGSYYSSGPAEVYFSGKATGIKDLTLVFDSGSSYTYFNSQAYNSILALVKNNLRGKP 300

Query: 301 LEDAPEDKSLPICWRGTHPFKSLHDVKKYFKPFALRFTKTKNTEMQLPPETYLIISKYGN 360
           LEDAPEDKSLP+CW+GT PFKSL DVKKYF P ALRFTKTKN ++QLPPE YLII+KYGN
Sbjct: 301 LEDAPEDKSLPVCWKGTRPFKSLRDVKKYFNPLALRFTKTKNAQIQLPPENYLIITKYGN 360

Query: 361 VCFGILNGSEVGLGDLNIIGDISLKDKLVIYDNERQRIGWFPTKCDKFRKEGQSLCRPEG 420
           VCFGILNG+EVGLGDLNIIGDISLKDK+VIYDNER+RIGWFPT C+KFRKEGQSLC+PEG
Sbjct: 361 VCFGILNGTEVGLGDLNIIGDISLKDKMVIYDNERRRIGWFPTNCNKFRKEGQSLCQPEG 420

Query: 421 IFSILSENYQGYIPKVF 438
           +FSIL+ENYQGYIPK+F
Sbjct: 421 LFSILTENYQGYIPKIF 437

BLAST of Cla97C08G148580 vs. ExPASy TrEMBL
Match: A0A5A7UPQ5 (Aspartic proteinase Asp1 OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold314G00190 PE=3 SV=1)

HSP 1 Score: 804.3 bits (2076), Expect = 2.5e-229
Identity = 381/437 (87.19%), Postives = 406/437 (92.91%), Query Frame = 0

Query: 1   MPTITTLIFFLLGLSSIFSVCFSTNILSLGKKNSDRLLSSAVFPLKGNVYPLGYYSVSIN 60
           M TIT+  FFLLGLSSIF V FSTNILSLGKKNSDRLLSS VFPLKGNVYPLGYYSVSIN
Sbjct: 1   MTTITSFFFFLLGLSSIFPVSFSTNILSLGKKNSDRLLSSVVFPLKGNVYPLGYYSVSIN 60

Query: 61  IGKRDEAFEFDIDSGSDLTWVQCDAPCTGCTKPREQLYKPDNNALNCFEPLCTSLHPIAD 120
           IGK DEAFEFDIDSGSDLTWVQCDAPCT CTKPREQLYKP+NNALNCFEPLC SLHPI +
Sbjct: 61  IGKGDEAFEFDIDSGSDLTWVQCDAPCTDCTKPREQLYKPNNNALNCFEPLCISLHPITN 120

Query: 121 HQCESADDHCQYEIEYADQGSSLGVLVNDNVPLKLTNGSLAAPRITFGCGYDHKYSVPHS 180
           H C+SADD CQYEIEYAD GSSLGVLVND+VPLKLTNGSLA PRI FGCGY+HKYSVP S
Sbjct: 121 HHCKSADDQCQYEIEYADHGSSLGVLVNDHVPLKLTNGSLATPRIAFGCGYEHKYSVPDS 180

Query: 181 PPPTAGVLGLGNGEVSIISQLSNMGVVRNVVGHCLSEEGGFLFFGDELVPSSGVTWTSMS 240
           PPPTAGVLGLGNGEVS ISQLSNMGVVRNVVGHCLSEEGGFLFFGDELVPSSGVTWTSMS
Sbjct: 181 PPPTAGVLGLGNGEVSFISQLSNMGVVRNVVGHCLSEEGGFLFFGDELVPSSGVTWTSMS 240

Query: 241 YESIGSYYSSGPAEVYFDGKATGIKDLILVFDSGSSYTYFNSQAYNAILALVRNDLSGKP 300
           +ESIG YYSSGPAEVYF GKATGIKDL LVFDSGSSYTYFNSQAYN+ILALVRN+L GKP
Sbjct: 241 HESIGGYYSSGPAEVYFGGKATGIKDLTLVFDSGSSYTYFNSQAYNSILALVRNNLRGKP 300

Query: 301 LEDAPEDKSLPICWRGTHPFKSLHDVKKYFKPFALRFTKTKNTEMQLPPETYLIISKYGN 360
           LEDAPEDKSLP+CW+G  PFKSLHDVKKYF P ALRFTKTK  ++QL PE YLII+KYGN
Sbjct: 301 LEDAPEDKSLPVCWKGPRPFKSLHDVKKYFNPLALRFTKTKKAQIQLAPEAYLIITKYGN 360

Query: 361 VCFGILNGSEVGLGDLNIIGDISLKDKLVIYDNERQRIGWFPTKCDKFRKEGQSLCRPEG 420
           VCFGILNG+EVGLG+LNIIGDISLKDK+VIYDNER++IGWFPT C+KF+KEGQSLC+PEG
Sbjct: 361 VCFGILNGTEVGLGNLNIIGDISLKDKMVIYDNERRQIGWFPTNCNKFKKEGQSLCQPEG 420

Query: 421 IFSILSENYQGYIPKVF 438
           +FSIL+ENYQGYI K+F
Sbjct: 421 LFSILTENYQGYIRKIF 437

BLAST of Cla97C08G148580 vs. ExPASy TrEMBL
Match: A0A1S3CRY8 (aspartic proteinase Asp1 OS=Cucumis melo OX=3656 GN=LOC103504073 PE=3 SV=1)

HSP 1 Score: 804.3 bits (2076), Expect = 2.5e-229
Identity = 381/437 (87.19%), Postives = 406/437 (92.91%), Query Frame = 0

Query: 1   MPTITTLIFFLLGLSSIFSVCFSTNILSLGKKNSDRLLSSAVFPLKGNVYPLGYYSVSIN 60
           M TIT+  FFLLGLSSIF V FSTNILSLGKKNSDRLLSS VFPLKGNVYPLGYYSVSIN
Sbjct: 1   MTTITSFFFFLLGLSSIFPVSFSTNILSLGKKNSDRLLSSVVFPLKGNVYPLGYYSVSIN 60

Query: 61  IGKRDEAFEFDIDSGSDLTWVQCDAPCTGCTKPREQLYKPDNNALNCFEPLCTSLHPIAD 120
           IGK DEAFEFDIDSGSDLTWVQCDAPCT CTKPREQLYKP+NNALNCFEPLC SLHPI +
Sbjct: 61  IGKGDEAFEFDIDSGSDLTWVQCDAPCTDCTKPREQLYKPNNNALNCFEPLCISLHPITN 120

Query: 121 HQCESADDHCQYEIEYADQGSSLGVLVNDNVPLKLTNGSLAAPRITFGCGYDHKYSVPHS 180
           H C+SADD CQYEIEYAD GSSLGVLVND+VPLKLTNGSLA PRI FGCGY+HKYSVP S
Sbjct: 121 HHCKSADDQCQYEIEYADHGSSLGVLVNDHVPLKLTNGSLATPRIAFGCGYEHKYSVPDS 180

Query: 181 PPPTAGVLGLGNGEVSIISQLSNMGVVRNVVGHCLSEEGGFLFFGDELVPSSGVTWTSMS 240
           PPPTAGVLGLGNGEVS ISQLSNMGVVRNVVGHCLSEEGGFLFFGDELVPSSGVTWTSMS
Sbjct: 181 PPPTAGVLGLGNGEVSFISQLSNMGVVRNVVGHCLSEEGGFLFFGDELVPSSGVTWTSMS 240

Query: 241 YESIGSYYSSGPAEVYFDGKATGIKDLILVFDSGSSYTYFNSQAYNAILALVRNDLSGKP 300
           +ESIG YYSSGPAEVYF GKATGIKDL LVFDSGSSYTYFNSQAYN+ILALVRN+L GKP
Sbjct: 241 HESIGGYYSSGPAEVYFGGKATGIKDLTLVFDSGSSYTYFNSQAYNSILALVRNNLRGKP 300

Query: 301 LEDAPEDKSLPICWRGTHPFKSLHDVKKYFKPFALRFTKTKNTEMQLPPETYLIISKYGN 360
           LEDAPEDKSLP+CW+G  PFKSLHDVKKYF P ALRFTKTK  ++QL PE YLII+KYGN
Sbjct: 301 LEDAPEDKSLPVCWKGPRPFKSLHDVKKYFNPLALRFTKTKKAQIQLAPEAYLIITKYGN 360

Query: 361 VCFGILNGSEVGLGDLNIIGDISLKDKLVIYDNERQRIGWFPTKCDKFRKEGQSLCRPEG 420
           VCFGILNG+EVGLG+LNIIGDISLKDK+VIYDNER++IGWFPT C+KF+KEGQSLC+PEG
Sbjct: 361 VCFGILNGTEVGLGNLNIIGDISLKDKMVIYDNERRQIGWFPTNCNKFKKEGQSLCQPEG 420

Query: 421 IFSILSENYQGYIPKVF 438
           +FSIL+ENYQGYI K+F
Sbjct: 421 LFSILTENYQGYIRKIF 437

BLAST of Cla97C08G148580 vs. ExPASy TrEMBL
Match: A0A6J1H0P2 (aspartic proteinase Asp1-like OS=Cucurbita moschata OX=3662 GN=LOC111458951 PE=3 SV=1)

HSP 1 Score: 760.0 bits (1961), Expect = 5.5e-216
Identity = 360/437 (82.38%), Postives = 389/437 (89.02%), Query Frame = 0

Query: 1   MPTITTLIFFLLGLSSIFSVCFSTNILSLGKKNSDRLLSSAVFPLKGNVYPLGYYSVSIN 60
           M    TLI FLL L + FSVCFS NI SL KKNSDRLLSSAVFPLKGNVYPLGYYSVSIN
Sbjct: 1   MARTPTLILFLLPLLATFSVCFSANIFSLRKKNSDRLLSSAVFPLKGNVYPLGYYSVSIN 60

Query: 61  IGKRDEAFEFDIDSGSDLTWVQCDAPCTGCTKPREQLYKPDNNALNCFEPLCTSLHPIAD 120
           IGK DEAFEFDIDSGSDLTWVQCDAPCTGCTKPR QLYKPDNNAL CFEPLC SLH  AD
Sbjct: 61  IGKGDEAFEFDIDSGSDLTWVQCDAPCTGCTKPRGQLYKPDNNALTCFEPLCASLHLTAD 120

Query: 121 HQCESADDHCQYEIEYADQGSSLGVLVNDNVPLKLTNGSLAAPRITFGCGYDHKYSVPHS 180
            QCESAD+ CQYEIEYAD GSSLGVLVND+ PLKLTNGSLAAPRI FGCGYDHKYSVP+ 
Sbjct: 121 QQCESADEQCQYEIEYADHGSSLGVLVNDHFPLKLTNGSLAAPRIAFGCGYDHKYSVPNP 180

Query: 181 PPPTAGVLGLGNGEVSIISQLSNMGVVRNVVGHCLSEEGGFLFFGDELVPSSGVTWTSMS 240
           PPPTAGVLGLGNGEVSIISQLSNMG++RNVVGHCLSE+GGFLFFGDEL+PSSGV WTSMS
Sbjct: 181 PPPTAGVLGLGNGEVSIISQLSNMGIMRNVVGHCLSEQGGFLFFGDELIPSSGVAWTSMS 240

Query: 241 YESIGSYYSSGPAEVYFDGKATGIKDLILVFDSGSSYTYFNSQAYNAILALVRNDLSGKP 300
            ESIG++YSSGPAEVYF GKA GIK L LVFDSGSSYTYF SQ Y+++LALVR+DL GKP
Sbjct: 241 SESIGNHYSSGPAEVYFGGKAAGIKGLTLVFDSGSSYTYFTSQVYDSVLALVRDDLKGKP 300

Query: 301 LEDAPEDKSLPICWRGTHPFKSLHDVKKYFKPFALRFTKTKNTEMQLPPETYLIISKYGN 360
           LEDAPEDKSLPICWRG  PFKSL DVK YF+P AL FTKTKN ++QLPPE+YLI++KYGN
Sbjct: 301 LEDAPEDKSLPICWRGPQPFKSLRDVKNYFRPLALHFTKTKNAQIQLPPESYLIVTKYGN 360

Query: 361 VCFGILNGSEVGLGDLNIIGDISLKDKLVIYDNERQRIGWFPTKCDKFRKEGQSLCRPEG 420
           VCFGILNG+EVGLGDLNIIGD+SL+DK+VIYDNER++IGWF T C+KF+KEGQS C PEG
Sbjct: 361 VCFGILNGTEVGLGDLNIIGDVSLQDKMVIYDNERRKIGWFSTSCNKFQKEGQSFCEPEG 420

Query: 421 IFSILSENYQGYIPKVF 438
             SILS NY  YIPK+F
Sbjct: 421 PLSILSGNYYEYIPKIF 437

BLAST of Cla97C08G148580 vs. ExPASy TrEMBL
Match: A0A6J1CK35 (aspartic proteinase Asp1-like OS=Momordica charantia OX=3673 GN=LOC111012010 PE=3 SV=1)

HSP 1 Score: 758.1 bits (1956), Expect = 2.1e-215
Identity = 361/442 (81.67%), Postives = 397/442 (89.82%), Query Frame = 0

Query: 1   MPTITTLIFFLLGLSSIFSVCFSTNILSLGKKNSDRLLSSAVFPLKGNVYPLGYYSVSIN 60
           MP  TTLI FLLGLSS F+VCFSTNILSLGK+ SDRLLSSAVFPLKGNVYPLGYYSVSIN
Sbjct: 1   MPRTTTLILFLLGLSSTFAVCFSTNILSLGKRKSDRLLSSAVFPLKGNVYPLGYYSVSIN 60

Query: 61  IGKRDEAFEFDIDSGSDLTWVQCDAPCTGCTKPREQLYKPDNNALNCFEPLCTSLHPIAD 120
           IGK  EAFEFDIDSGSDLTWVQCDAPCTGCTKPREQLYKPDNNALNCFEPLC SL P  +
Sbjct: 61  IGKGTEAFEFDIDSGSDLTWVQCDAPCTGCTKPREQLYKPDNNALNCFEPLCASLRPTTN 120

Query: 121 HQCESADDHCQYEIEYADQGSSLGVLVNDNVPLKLTNGSLAAPRITFGCGYDHKYSVPHS 180
           HQCESAD+ C+YEIEYAD GSSLGVLVND VPLKLTNGSLAAPRITFGCGYDHKYS+PHS
Sbjct: 121 HQCESADEQCEYEIEYADYGSSLGVLVNDQVPLKLTNGSLAAPRITFGCGYDHKYSLPHS 180

Query: 181 PPPTAGVLGLGNGEVSIISQLSNMGVVRNVVGHCLSEEGGFLFFGDELVPSSGVTWTSMS 240
           PPPT+GVLGLGNGEVSIISQLS MGVVRNV+GHCLSE+GGF+FFGDEL+PSSGV WTSMS
Sbjct: 181 PPPTSGVLGLGNGEVSIISQLSKMGVVRNVLGHCLSEQGGFIFFGDELIPSSGVAWTSMS 240

Query: 241 YESIGSYYSSGPAEVYFDGKATGIKDLILVFDSGSSYTYFNSQAYNAILALVRNDLSGKP 300
           YESIG+YYSSGPAEVYF GKA GI+DL +VFDSGSSYTYF+SQ Y+ ILA+VR DL GKP
Sbjct: 241 YESIGNYYSSGPAEVYFGGKAVGIEDLTVVFDSGSSYTYFSSQVYDRILAMVREDLRGKP 300

Query: 301 LEDAPEDKSLPICWRGTHPFKSLHDVKKYFKPFALRFTKTKNTEMQLPPETYLIISKYGN 360
           L+DAPED+SLPICWRGTHPF+SL DVK YFKP AL FT +KN+++ LPPETYLII+KYGN
Sbjct: 301 LKDAPEDESLPICWRGTHPFESLLDVKNYFKPLALSFTNSKNSQILLPPETYLIITKYGN 360

Query: 361 VCFGILNGSEVGLGDLNIIGDISLKDKLVIYDNERQRIGWFPTKCDKFRKEG----QSLC 420
           VCFGILNG+EVGLGDLN+IGDISL+DK+VIYDNE+Q+IGW  TKC KFRKE     Q LC
Sbjct: 361 VCFGILNGTEVGLGDLNVIGDISLQDKMVIYDNEKQQIGWVSTKCSKFRKEDQGFEQGLC 420

Query: 421 R-PEGIFSILSENYQGYIPKVF 438
           +  EG FSIL+ +Y+ Y  K+F
Sbjct: 421 QSEEGPFSILTGHYREYASKIF 442

BLAST of Cla97C08G148580 vs. TAIR 10
Match: AT1G77480.2 (Eukaryotic aspartyl protease family protein )

HSP 1 Score: 480.3 bits (1235), Expect = 1.6e-135
Identity = 232/413 (56.17%), Postives = 292/413 (70.70%), Query Frame = 0

Query: 7   LIFFLLGLSSIFSVC--FSTNILSLG-----KKNSDRLLSSAVFPLKGNVYPLGYYSVSI 66
           L FF + +  +F +C  F T+  +       K  + RL S+ VFP+ GNVYPLGYY V +
Sbjct: 12  LYFFFVFVFYVFILCARFQTSEATKDSSAQVKLQNRRLSSTVVFPVSGNVYPLGYYYVLL 71

Query: 67  NIGKRDEAFEFDIDSGSDLTWVQCDAPCTGCTKPREQLYKPDNNALNCFEPLCTSLHPIA 126
           NIG   + F+ DID+GSDLTWVQCDAPC GCTKPR + YKP++N L C   LC+ L    
Sbjct: 72  NIGNPPKLFDLDIDTGSDLTWVQCDAPCNGCTKPRAKQYKPNHNTLPCSHILCSGLDLPQ 131

Query: 127 DHQCESADDHCQYEIEYADQGSSLGVLVNDNVPLKLTNGSLAAPRITFGCGYDHKYSVPH 186
           D  C   +D C YEI Y+D  SS+G LV D VPLKL NGS+   R+TFGCGYD +   PH
Sbjct: 132 DRPCADPEDQCDYEIGYSDHASSIGALVTDEVPLKLANGSIMNLRLTFGCGYDQQNPGPH 191

Query: 187 SPPPTAGVLGLGNGEVSIISQLSNMGVVRNVVGHCLSEEG-GFLFFGDELVPSSGVTWTS 246
            PPPTAG+LGLG G+V + +QL ++G+ +NV+ HCLS  G GFL  GDELVPSSGVTWTS
Sbjct: 192 PPPPTAGILGLGRGKVGLSTQLKSLGITKNVIVHCLSHTGKGFLSIGDELVPSSGVTWTS 251

Query: 247 MSYESIGSYYSSGPAEVYFDGKATGIKDLILVFDSGSSYTYFNSQAYNAILALVRNDLSG 306
           ++  S    Y +GPAE+ F+ K TG+K + +VFDSGSSYTYFN++AY AIL L+R DL+G
Sbjct: 252 LATNSPSKNYMAGPAELLFNDKTTGVKGINVVFDSGSSYTYFNAEAYQAILDLIRKDLNG 311

Query: 307 KPLEDAPEDKSLPICWRGTHPFKSLHDVKKYFKPFALRFTKTKNTEM-QLPPETYLIISK 366
           KPL D  +DKSLP+CW+G  P KSL +VKKYFK   LRF   KN ++ Q+PPE+YLII++
Sbjct: 312 KPLTDTKDDKSLPVCWKGKKPLKSLDEVKKYFKTITLRFGNQKNGQLFQVPPESYLIITE 371

Query: 367 YGNVCFGILNGSEVGLGDLNIIGDISLKDKLVIYDNERQRIGWFPTKCDKFRK 411
            G VC GILNG+E+GL   NIIGDIS +  +VIYDNE+QRIGW  + CDK  K
Sbjct: 372 KGRVCLGILNGTEIGLEGYNIIGDISFQGIMVIYDNEKQRIGWISSDCDKLPK 424

BLAST of Cla97C08G148580 vs. TAIR 10
Match: AT1G77480.1 (Eukaryotic aspartyl protease family protein )

HSP 1 Score: 479.2 bits (1232), Expect = 3.6e-135
Identity = 231/410 (56.34%), Postives = 291/410 (70.98%), Query Frame = 0

Query: 7   LIFFLLGLSSIFSVC--FSTNILSLG-----KKNSDRLLSSAVFPLKGNVYPLGYYSVSI 66
           L FF + +  +F +C  F T+  +       K  + RL S+ VFP+ GNVYPLGYY V +
Sbjct: 12  LYFFFVFVFYVFILCARFQTSEATKDSSAQVKLQNRRLSSTVVFPVSGNVYPLGYYYVLL 71

Query: 67  NIGKRDEAFEFDIDSGSDLTWVQCDAPCTGCTKPREQLYKPDNNALNCFEPLCTSLHPIA 126
           NIG   + F+ DID+GSDLTWVQCDAPC GCTKPR + YKP++N L C   LC+ L    
Sbjct: 72  NIGNPPKLFDLDIDTGSDLTWVQCDAPCNGCTKPRAKQYKPNHNTLPCSHILCSGLDLPQ 131

Query: 127 DHQCESADDHCQYEIEYADQGSSLGVLVNDNVPLKLTNGSLAAPRITFGCGYDHKYSVPH 186
           D  C   +D C YEI Y+D  SS+G LV D VPLKL NGS+   R+TFGCGYD +   PH
Sbjct: 132 DRPCADPEDQCDYEIGYSDHASSIGALVTDEVPLKLANGSIMNLRLTFGCGYDQQNPGPH 191

Query: 187 SPPPTAGVLGLGNGEVSIISQLSNMGVVRNVVGHCLSEEG-GFLFFGDELVPSSGVTWTS 246
            PPPTAG+LGLG G+V + +QL ++G+ +NV+ HCLS  G GFL  GDELVPSSGVTWTS
Sbjct: 192 PPPPTAGILGLGRGKVGLSTQLKSLGITKNVIVHCLSHTGKGFLSIGDELVPSSGVTWTS 251

Query: 247 MSYESIGSYYSSGPAEVYFDGKATGIKDLILVFDSGSSYTYFNSQAYNAILALVRNDLSG 306
           ++  S    Y +GPAE+ F+ K TG+K + +VFDSGSSYTYFN++AY AIL L+R DL+G
Sbjct: 252 LATNSPSKNYMAGPAELLFNDKTTGVKGINVVFDSGSSYTYFNAEAYQAILDLIRKDLNG 311

Query: 307 KPLEDAPEDKSLPICWRGTHPFKSLHDVKKYFKPFALRFTKTKNTEM-QLPPETYLIISK 366
           KPL D  +DKSLP+CW+G  P KSL +VKKYFK   LRF   KN ++ Q+PPE+YLII++
Sbjct: 312 KPLTDTKDDKSLPVCWKGKKPLKSLDEVKKYFKTITLRFGNQKNGQLFQVPPESYLIITE 371

Query: 367 YGNVCFGILNGSEVGLGDLNIIGDISLKDKLVIYDNERQRIGWFPTKCDK 408
            G VC GILNG+E+GL   NIIGDIS +  +VIYDNE+QRIGW  + CDK
Sbjct: 372 KGRVCLGILNGTEIGLEGYNIIGDISFQGIMVIYDNEKQRIGWISSDCDK 421

BLAST of Cla97C08G148580 vs. TAIR 10
Match: AT1G44130.1 (Eukaryotic aspartyl protease family protein )

HSP 1 Score: 465.7 bits (1197), Expect = 4.1e-131
Identity = 214/374 (57.22%), Postives = 274/374 (73.26%), Query Frame = 0

Query: 39  SSAVFPLKGNVYPLGYYSVSINIGKRDEAFEFDIDSGSDLTWVQCDAPCTGCTKPREQLY 98
           SS VFPL GNV+PLGYYSV + IG   +AF+FDID+GSDLTWVQCDAPC+GCT P    Y
Sbjct: 33  SSVVFPLSGNVFPLGYYSVLMQIGSPPKAFQFDIDTGSDLTWVQCDAPCSGCTLPPNLQY 92

Query: 99  KPDNNALNCFEPLCTSLHPIADHQCESADDHCQYEIEYADQGSSLGVLVNDNVPLKLTNG 158
           KP  N + C  P+CT+LH      C +  + C YE++YADQGSS+G LV D  PLKL NG
Sbjct: 93  KPKGNIIPCSNPICTALHWPNKPHCPNPQEQCDYEVKYADQGSSMGALVTDQFPLKLVNG 152

Query: 159 SLAAPRITFGCGYDHKYSVPHSPPPTAGVLGLGNGEVSIISQLSNMGVVRNVVGHCLSEE 218
           S   P + FGCGYD  Y   H PP TAGVLGLG G++ +++QL + G+ RNVVGHCLS +
Sbjct: 153 SFMQPPVAFGCGYDQSYPSAHPPPATAGVLGLGRGKIGLLTQLVSAGLTRNVVGHCLSSK 212

Query: 219 -GGFLFFGDELVPSSGVTWTSMSYESIGSYYSSGPAEVYFDGKATGIKDLILVFDSGSSY 278
            GGFLFFGD LVPS GV WT +   S  ++Y++GPA++ F+GK TG+K L L+FD+GSSY
Sbjct: 213 GGGFLFFGDNLVPSIGVAWTPLL--SQDNHYTTGPADLLFNGKPTGLKGLKLIFDTGSSY 272

Query: 279 TYFNSQAYNAILALVRNDLSGKPLEDAPEDKSLPICWRGTHPFKSLHDVKKYFKPFALRF 338
           TYFNS+AY  I+ L+ NDL   PL+ A EDK+LPICW+G  PFKS+ +VK +FK   + F
Sbjct: 273 TYFNSKAYQTIINLIGNDLKVSPLKVAKEDKTLPICWKGAKPFKSVLEVKNFFKTITINF 332

Query: 339 TK-TKNTEMQLPPETYLIISKYGNVCFGILNGSEVGLGDLNIIGDISLKDKLVIYDNERQ 398
           T   +NT++ L PE YLI+SK GNVC G+LNGSEVGL + N+IGDIS++  ++IYDNE+Q
Sbjct: 333 TNGRRNTQLYLAPELYLIVSKTGNVCLGLLNGSEVGLQNSNVIGDISMQGLMMIYDNEKQ 392

Query: 399 RIGWFPTKCDKFRK 411
           ++GW  + C+K  K
Sbjct: 393 QLGWVSSDCNKLPK 404

BLAST of Cla97C08G148580 vs. TAIR 10
Match: AT4G33490.2 (Eukaryotic aspartyl protease family protein )

HSP 1 Score: 428.7 bits (1101), Expect = 5.6e-120
Identity = 203/375 (54.13%), Postives = 267/375 (71.20%), Query Frame = 0

Query: 36  RLLSSAVFPLKGNVYPLGYYSVSINIGKRDEAFEFDIDSGSDLTWVQCDAPCTGCTKPRE 95
           R +SS VFP+ GNVYPLGYY+V+INIG+    +  D+D+GSDLTW+QCDAPC  C +   
Sbjct: 41  RAVSSVVFPVHGNVYPLGYYNVTINIGQPPRPYYLDLDTGSDLTWLQCDAPCVRCLEAPH 100

Query: 96  QLYKPDNNALNCFEPLCTSLHPIADHQCESADDHCQYEIEYADQGSSLGVLVNDNVPLKL 155
            LY+P ++ + C +PLC +LH  ++ +CE+  + C YE+EYAD GSSLGVLV D   +  
Sbjct: 101 PLYQPSSDLIPCNDPLCKALHLNSNQRCET-PEQCDYEVEYADGGSSLGVLVRDVFSMNY 160

Query: 156 TNGSLAAPRITFGCGYDHKYSVPHSPPPTAGVLGLGNGEVSIISQLSNMGVVRNVVGHCL 215
           T G    PR+  GCGYD +     S  P  GVLGLG G+VSI+SQL + G V+NV+GHCL
Sbjct: 161 TQGLRLTPRLALGCGYD-QIPGASSHHPLDGVLGLGRGKVSILSQLHSQGYVKNVIGHCL 220

Query: 216 SE-EGGFLFFGDELVPSSGVTWTSMSYESIGSYYSSGPAEVYFDGKATGIKDLILVFDSG 275
           S   GG LFFGD+L  SS V+WT MS E    Y  +   E+ F G+ TG+K+L+ VFDSG
Sbjct: 221 SSLGGGILFFGDDLYDSSRVSWTPMSREYSKHYSPAMGGELLFGGRTTGLKNLLTVFDSG 280

Query: 276 SSYTYFNSQAYNAILALVRNDLSGKPLEDAPEDKSLPICWRGTHPFKSLHDVKKYFKPFA 335
           SSYTYFNS+AY A+  L++ +LSGKPL++A +D +LP+CW+G  PF S+ +VKKYFKP A
Sbjct: 281 SSYTYFNSKAYQAVTYLLKRELSGKPLKEARDDHTLPLCWQGRRPFMSIEEVKKYFKPLA 340

Query: 336 LRFTK--TKNTEMQLPPETYLIISKYGNVCFGILNGSEVGLGDLNIIGDISLKDKLVIYD 395
           L F       T  ++PPE YLIIS  GNVC GILNG+E+GL +LN+IGDIS++D+++IYD
Sbjct: 341 LSFKTGWRSKTLFEIPPEAYLIISMKGNVCLGILNGTEIGLQNLNLIGDISMQDQMIIYD 400

Query: 396 NERQRIGWFPTKCDK 408
           NE+Q IGW P  CD+
Sbjct: 401 NEKQSIGWMPVDCDE 413

BLAST of Cla97C08G148580 vs. TAIR 10
Match: AT4G33490.1 (Eukaryotic aspartyl protease family protein )

HSP 1 Score: 386.3 bits (991), Expect = 3.2e-107
Identity = 187/348 (53.74%), Postives = 244/348 (70.11%), Query Frame = 0

Query: 36  RLLSSAVFPLKGNVYPLGYYSVSINIGKRDEAFEFDIDSGSDLTWVQCDAPCTGCTKPRE 95
           R +SS VFP+ GNVYPLGYY+V+INIG+    +  D+D+GSDLTW+QCDAPC  C +   
Sbjct: 38  RAVSSVVFPVHGNVYPLGYYNVTINIGQPPRPYYLDLDTGSDLTWLQCDAPCVRCLEAPH 97

Query: 96  QLYKPDNNALNCFEPLCTSLHPIADHQCESADDHCQYEIEYADQGSSLGVLVNDNVPLKL 155
            LY+P ++ + C +PLC +LH  ++ +CE+  + C YE+EYAD GSSLGVLV D   +  
Sbjct: 98  PLYQPSSDLIPCNDPLCKALHLNSNQRCET-PEQCDYEVEYADGGSSLGVLVRDVFSMNY 157

Query: 156 TNGSLAAPRITFGCGYDHKYSVPHSPPPTAGVLGLGNGEVSIISQLSNMGVVRNVVGHCL 215
           T G    PR+  GCGYD +     S  P  GVLGLG G+VSI+SQL + G V+NV+GHCL
Sbjct: 158 TQGLRLTPRLALGCGYD-QIPGASSHHPLDGVLGLGRGKVSILSQLHSQGYVKNVIGHCL 217

Query: 216 SE-EGGFLFFGDELVPSSGVTWTSMSYESIGSYYSSGPAEVYFDGKATGIKDLILVFDSG 275
           S   GG LFFGD+L  SS V+WT MS E    Y  +   E+ F G+ TG+K+L+ VFDSG
Sbjct: 218 SSLGGGILFFGDDLYDSSRVSWTPMSREYSKHYSPAMGGELLFGGRTTGLKNLLTVFDSG 277

Query: 276 SSYTYFNSQAYNAILALVRNDLSGKPLEDAPEDKSLPICWRGTHPFKSLHDVKKYFKPFA 335
           SSYTYFNS+AY A+  L++ +LSGKPL++A +D +LP+CW+G  PF S+ +VKKYFKP A
Sbjct: 278 SSYTYFNSKAYQAVTYLLKRELSGKPLKEARDDHTLPLCWQGRRPFMSIEEVKKYFKPLA 337

Query: 336 LRFTK--TKNTEMQLPPETYLIISKYGNVCFGILNGSEVGLGDLNIIG 381
           L F       T  ++PPE YLIIS  GNVC GILNG+E+GL +LN+IG
Sbjct: 338 LSFKTGWRSKTLFEIPPEAYLIISMKGNVCLGILNGTEIGLQNLNLIG 383

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
XP_038885447.16.4e-23589.24aspartic proteinase Asp1-like [Benincasa hispida][more]
XP_004149806.14.3e-23188.10aspartic proteinase Asp1 [Cucumis sativus] >XP_011657431.1 aspartic proteinase A... [more]
XP_008466731.15.2e-22987.19PREDICTED: aspartic proteinase Asp1 [Cucumis melo] >KAA0055449.1 aspartic protei... [more]
KAG6602107.11.3e-21682.61Aspartyl protease APCB1, partial [Cucurbita argyrosperma subsp. sororia][more]
XP_023524501.13.0e-21682.61aspartic proteinase Asp1-like [Cucurbita pepo subsp. pepo][more]
Match NameE-valueIdentityDescription
Q9M9A81.3e-8443.11Aspartyl protease APCB1 OS=Arabidopsis thaliana OX=3702 GN=APCB1 PE=1 SV=1[more]
Q0IU521.2e-8241.65Aspartic proteinase Asp1 OS=Oryza sativa subsp. japonica OX=39947 GN=ASP1 PE=2 S... [more]
A2ZC672.1e-7940.87Aspartic proteinase Asp1 OS=Oryza sativa subsp. indica OX=39946 GN=ASP1 PE=2 SV=... [more]
Q9S9K44.0e-3025.89Aspartic proteinase 39 OS=Arabidopsis thaliana OX=3702 GN=A39 PE=1 SV=2[more]
Q4V3D28.3e-2825.38Aspartic proteinase 36 OS=Arabidopsis thaliana OX=3702 GN=A36 PE=1 SV=1[more]
Match NameE-valueIdentityDescription
A0A0A0KH372.1e-23188.10Peptidase A1 domain-containing protein OS=Cucumis sativus OX=3659 GN=Csa_6G38300... [more]
A0A5A7UPQ52.5e-22987.19Aspartic proteinase Asp1 OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffol... [more]
A0A1S3CRY82.5e-22987.19aspartic proteinase Asp1 OS=Cucumis melo OX=3656 GN=LOC103504073 PE=3 SV=1[more]
A0A6J1H0P25.5e-21682.38aspartic proteinase Asp1-like OS=Cucurbita moschata OX=3662 GN=LOC111458951 PE=3... [more]
A0A6J1CK352.1e-21581.67aspartic proteinase Asp1-like OS=Momordica charantia OX=3673 GN=LOC111012010 PE=... [more]
Match NameE-valueIdentityDescription
AT1G77480.21.6e-13556.17Eukaryotic aspartyl protease family protein [more]
AT1G77480.13.6e-13556.34Eukaryotic aspartyl protease family protein [more]
AT1G44130.14.1e-13157.22Eukaryotic aspartyl protease family protein [more]
AT4G33490.25.6e-12054.13Eukaryotic aspartyl protease family protein [more]
AT4G33490.13.2e-10753.74Eukaryotic aspartyl protease family protein [more]
InterPro
Analysis Name: InterPro Annotations of Watermelon (97103) v2.5
Date Performed: 2022-01-31
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR032799Xylanase inhibitor, C-terminalPFAMPF14541TAXi_Ccoord: 269..400
e-value: 1.7E-14
score: 53.9
IPR021109Aspartic peptidase domain superfamilyGENE3D2.40.70.10Acid Proteasescoord: 31..226
e-value: 7.6E-41
score: 142.2
IPR021109Aspartic peptidase domain superfamilyGENE3D2.40.70.10Acid Proteasescoord: 231..411
e-value: 6.0E-28
score: 99.5
IPR021109Aspartic peptidase domain superfamilySUPERFAMILY50630Acid proteasescoord: 47..407
IPR032861Xylanase inhibitor, N-terminalPFAMPF14543TAXi_Ncoord: 55..226
e-value: 4.3E-42
score: 144.3
IPR001461Aspartic peptidase A1 familyPANTHERPTHR13683ASPARTYL PROTEASEScoord: 5..414
NoneNo IPR availablePANTHERPTHR13683:SF796EUKARYOTIC ASPARTYL PROTEASE FAMILY PROTEINcoord: 5..414
IPR001969Aspartic peptidase, active sitePROSITEPS00141ASP_PROTEASEcoord: 70..81
IPR033121Peptidase family A1 domainPROSITEPS51767PEPTIDASE_A1coord: 55..401
score: 34.707317

Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
Cla97C08G148580.1Cla97C08G148580.1mRNA


GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
biological_process GO:0006508 proteolysis
molecular_function GO:0004190 aspartic-type endopeptidase activity