Cla97C08G148380 (gene) Watermelon (97103) v2.5

Overview
NameCla97C08G148380
Typegene
OrganismCitrullus lanatus subsp. vulgaris cv. 97103 (Watermelon (97103) v2.5)
DescriptionEukaryotic aspartyl protease family protein
LocationCla97Chr08: 16432332 .. 16434018 (+)
RNA-Seq ExpressionCla97C08G148380
SyntenyCla97C08G148380
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: exonfive_prime_UTRCDSpolypeptidethree_prime_UTR
Hold the cursor over a type above to highlight its positions in the sequence below.
CTTCCTTCCACTATGTGGACGGTTGCCAATATAAAACCTTCATTTGTAACCTTTTTCAAATAAGATCCTCTGCTTCTGATGCCAATGGCAAATTGTTGCTTCTTGTTACTGCTTTTGTTGCCAGTTCTCCATTCCCAGCCAGCTTTCAGTGAAAGTTCACTAAAACCTGGATTTCATTTTGATCTTACACATGTTGATGCTACCCAAAACTTCACCAAATTCGAAATGCTCCGACGAGGAATTGAGCGAGACAAGACCCGGATACAAAATTTCAAAAAGATGACTGTAAACTTCGAAGTTCGAATGCCACTCGTTGACCAACAAGGGAGCTATCTGATGAACTTGTCATTTGGAACACCTCCTGTTTCGTTCTCTGCAATTTTCGATACTGGCAGTGACTTGATTTGGACACAGTGCAATCCATGCTTGGAATGTTTTGGACAACCAACCCCCGTGTATGATCCTGCACAATCGTCTTCCTTCACCAATGCTACTCGCTCCGCTGCATTATGCCAGGCTTTGACCAATTCAAGTTCCAGCAATGGTTGTGAGTACTCATATGGTTATGGAGATGGATCTTTCACCATAGGTTATTTGGCGTTCGAGACCTTGACGCTTGGGGAAGCAAACCAGCAAGCTTCTACACCAGACATAGCTTTTGGGTGCAGGATAAGGAACTACGTAAACGGCTTGACGCAGGGTGCTGGCATAGTAGGGTTCAGCCGAGGACCATTGTCGCTTCTTTCGCAGCTCCATATTCGAAAGTTCTCTTACTGTTTAAGTCCAAATGGAACTGGGTGTCTGGCCTTTGGATCATCTTCAACAAGCTTTGACAACACAACAAACCAAGCCGTCAAGAACACGCCACTGATACAAAATCCATCCAATCCATCTTACTACTATCTATCTCTACAAGGAATCACAGTTGGCCAAAAGTTTTTGCCCGTCCCATCCTCATGGTTTGAACTAAATGCTGATGGCAGCGGCGGCGTGATCATAGATTCTGACACATCGATCACGTATATAACAGAGGATGCCTTTGATGTGCTGAAGCCAGTTTTTACTTCACAGACAAACCTCCCGGTGATAAATTCAGCAAGTATTGGTCTTGACCTCTGCTTTGAGCTACCTTCCCCAAACAATAGTAAACTTGATGTGCCTGATTTGATATTTCGTTTTGAGGGTCTCGACTTGAAGCTTCCAGTCGAGAACTATATGGTCGTCAATGAGGAGGTAGGGATTGTATGCTTGGCAATGGGGGCTGCAGGAGGTTTGTCGATTTTCGGCAGCATGCAACATCAAAATATGTTGGTTCTTCATGATCTCAAGAAAAAAGTCATATCATTCATTCCCACACAATGCTCTCGGATCAATTACTAGGAGTCAGAACTCGAAGATCACATGCATCATTTGAATAACATCAGTCCTACCGGTGCTTTGCTCTCTGTTATCTAGCATCTGAAGCAGTAAGACATTAACATATTGCAAAACTTTTCTAAATAGCATAGTGTCTAACTGGCAACATATTTTCTCATCTCAATGACTTAAACTAGCAAGGTTGGAATGGATGTAACAAATAGTAAATAAATCAAGCTTACATAGACAATAACAGCAATGTGCAGTATCTTCTAAGACAGTACAATTGCAAGAAAACAAAGAAATGATCTCAAAAAACCTTTAGTCCGCC

mRNA sequence

CTTCCTTCCACTATGTGGACGGTTGCCAATATAAAACCTTCATTTGTAACCTTTTTCAAATAAGATCCTCTGCTTCTGATGCCAATGGCAAATTGTTGCTTCTTGTTACTGCTTTTGTTGCCAGTTCTCCATTCCCAGCCAGCTTTCAGTGAAAGTTCACTAAAACCTGGATTTCATTTTGATCTTACACATGTTGATGCTACCCAAAACTTCACCAAATTCGAAATGCTCCGACGAGGAATTGAGCGAGACAAGACCCGGATACAAAATTTCAAAAAGATGACTGTAAACTTCGAAGTTCGAATGCCACTCGTTGACCAACAAGGGAGCTATCTGATGAACTTGTCATTTGGAACACCTCCTGTTTCGTTCTCTGCAATTTTCGATACTGGCAGTGACTTGATTTGGACACAGTGCAATCCATGCTTGGAATGTTTTGGACAACCAACCCCCGTGTATGATCCTGCACAATCGTCTTCCTTCACCAATGCTACTCGCTCCGCTGCATTATGCCAGGCTTTGACCAATTCAAGTTCCAGCAATGGTTGTGAGTACTCATATGGTTATGGAGATGGATCTTTCACCATAGGTTATTTGGCGTTCGAGACCTTGACGCTTGGGGAAGCAAACCAGCAAGCTTCTACACCAGACATAGCTTTTGGGTGCAGGATAAGGAACTACGTAAACGGCTTGACGCAGGGTGCTGGCATAGTAGGGTTCAGCCGAGGACCATTGTCGCTTCTTTCGCAGCTCCATATTCGAAAGTTCTCTTACTGTTTAAGTCCAAATGGAACTGGGTGTCTGGCCTTTGGATCATCTTCAACAAGCTTTGACAACACAACAAACCAAGCCGTCAAGAACACGCCACTGATACAAAATCCATCCAATCCATCTTACTACTATCTATCTCTACAAGGAATCACAGTTGGCCAAAAGTTTTTGCCCGTCCCATCCTCATGGTTTGAACTAAATGCTGATGGCAGCGGCGGCGTGATCATAGATTCTGACACATCGATCACGTATATAACAGAGGATGCCTTTGATGTGCTGAAGCCAGTTTTTACTTCACAGACAAACCTCCCGGTGATAAATTCAGCAAGTATTGGTCTTGACCTCTGCTTTGAGCTACCTTCCCCAAACAATAGTAAACTTGATGTGCCTGATTTGATATTTCGTTTTGAGGGTCTCGACTTGAAGCTTCCAGTCGAGAACTATATGGTCGTCAATGAGGAGGTAGGGATTGTATGCTTGGCAATGGGGGCTGCAGGAGGTTTGTCGATTTTCGGCAGCATGCAACATCAAAATATGTTGGTTCTTCATGATCTCAAGAAAAAAGTCATATCATTCATTCCCACACAATGCTCTCGGATCAATTACTAGGAGTCAGAACTCGAAGATCACATGCATCATTTGAATAACATCAGTCCTACCGGTGCTTTGCTCTCTGTTATCTAGCATCTGAAGCAGTAAGACATTAACATATTGCAAAACTTTTCTAAATAGCATAGTGTCTAACTGGCAACATATTTTCTCATCTCAATGACTTAAACTAGCAAGGTTGGAATGGATGTAACAAATAGTAAATAAATCAAGCTTACATAGACAATAACAGCAATGTGCAGTATCTTCTAAGACAGTACAATTGCAAGAAAACAAAGAAATGATCTCAAAAAACCTTTAGTCCGCC

Coding sequence (CDS)

ATGCCAATGGCAAATTGTTGCTTCTTGTTACTGCTTTTGTTGCCAGTTCTCCATTCCCAGCCAGCTTTCAGTGAAAGTTCACTAAAACCTGGATTTCATTTTGATCTTACACATGTTGATGCTACCCAAAACTTCACCAAATTCGAAATGCTCCGACGAGGAATTGAGCGAGACAAGACCCGGATACAAAATTTCAAAAAGATGACTGTAAACTTCGAAGTTCGAATGCCACTCGTTGACCAACAAGGGAGCTATCTGATGAACTTGTCATTTGGAACACCTCCTGTTTCGTTCTCTGCAATTTTCGATACTGGCAGTGACTTGATTTGGACACAGTGCAATCCATGCTTGGAATGTTTTGGACAACCAACCCCCGTGTATGATCCTGCACAATCGTCTTCCTTCACCAATGCTACTCGCTCCGCTGCATTATGCCAGGCTTTGACCAATTCAAGTTCCAGCAATGGTTGTGAGTACTCATATGGTTATGGAGATGGATCTTTCACCATAGGTTATTTGGCGTTCGAGACCTTGACGCTTGGGGAAGCAAACCAGCAAGCTTCTACACCAGACATAGCTTTTGGGTGCAGGATAAGGAACTACGTAAACGGCTTGACGCAGGGTGCTGGCATAGTAGGGTTCAGCCGAGGACCATTGTCGCTTCTTTCGCAGCTCCATATTCGAAAGTTCTCTTACTGTTTAAGTCCAAATGGAACTGGGTGTCTGGCCTTTGGATCATCTTCAACAAGCTTTGACAACACAACAAACCAAGCCGTCAAGAACACGCCACTGATACAAAATCCATCCAATCCATCTTACTACTATCTATCTCTACAAGGAATCACAGTTGGCCAAAAGTTTTTGCCCGTCCCATCCTCATGGTTTGAACTAAATGCTGATGGCAGCGGCGGCGTGATCATAGATTCTGACACATCGATCACGTATATAACAGAGGATGCCTTTGATGTGCTGAAGCCAGTTTTTACTTCACAGACAAACCTCCCGGTGATAAATTCAGCAAGTATTGGTCTTGACCTCTGCTTTGAGCTACCTTCCCCAAACAATAGTAAACTTGATGTGCCTGATTTGATATTTCGTTTTGAGGGTCTCGACTTGAAGCTTCCAGTCGAGAACTATATGGTCGTCAATGAGGAGGTAGGGATTGTATGCTTGGCAATGGGGGCTGCAGGAGGTTTGTCGATTTTCGGCAGCATGCAACATCAAAATATGTTGGTTCTTCATGATCTCAAGAAAAAAGTCATATCATTCATTCCCACACAATGCTCTCGGATCAATTACTAG

Protein sequence

MPMANCCFLLLLLLPVLHSQPAFSESSLKPGFHFDLTHVDATQNFTKFEMLRRGIERDKTRIQNFKKMTVNFEVRMPLVDQQGSYLMNLSFGTPPVSFSAIFDTGSDLIWTQCNPCLECFGQPTPVYDPAQSSSFTNATRSAALCQALTNSSSSNGCEYSYGYGDGSFTIGYLAFETLTLGEANQQASTPDIAFGCRIRNYVNGLTQGAGIVGFSRGPLSLLSQLHIRKFSYCLSPNGTGCLAFGSSSTSFDNTTNQAVKNTPLIQNPSNPSYYYLSLQGITVGQKFLPVPSSWFELNADGSGGVIIDSDTSITYITEDAFDVLKPVFTSQTNLPVINSASIGLDLCFELPSPNNSKLDVPDLIFRFEGLDLKLPVENYMVVNEEVGIVCLAMGAAGGLSIFGSMQHQNMLVLHDLKKKVISFIPTQCSRINY
Homology
BLAST of Cla97C08G148380 vs. NCBI nr
Match: XP_038886455.1 (aspartic proteinase nepenthesin-2-like [Benincasa hispida])

HSP 1 Score: 724.2 bits (1868), Expect = 6.8e-205
Identity = 362/434 (83.41%), Postives = 394/434 (90.78%), Query Frame = 0

Query: 1   MPMANCCFLLLLLLPVLHSQPAFSESSLKPGFHFDLTHVDATQNFTKFEMLRRGIERDKT 60
           MPMAN CFLLLLLLP+L S+PAFSESSLKPGFHFDLTHVDAT NF+KFE+LRRGI+RDKT
Sbjct: 1   MPMANFCFLLLLLLPLLSSKPAFSESSLKPGFHFDLTHVDATWNFSKFEILRRGIKRDKT 60

Query: 61  RIQNFKKMTV-NFEVRMPLVDQQGSYLMNLSFGTPPVSFSAIFDTGSDLIWTQCNPCLEC 120
            IQNF KM + N +VRMP+V+Q+GSYLMNLS GTPP+SFSAIFDTGSDL+WTQC PCL+C
Sbjct: 61  WIQNFNKMIISNLKVRMPVVEQRGSYLMNLSLGTPPISFSAIFDTGSDLVWTQCKPCLQC 120

Query: 121 FGQPTPVYDPAQSSSFTNATRSAALCQALTNSSSSNGCEYSYGYGDGSFTIGYLAFETLT 180
           F QPTPVYDPAQSSSF NATRSAALC+ LTNSSSSNGCEYS+ Y D SFT GYLAFETLT
Sbjct: 121 FKQPTPVYDPAQSSSFANATRSAALCKELTNSSSSNGCEYSFSYADESFTTGYLAFETLT 180

Query: 181 LGEANQQASTPDIAFGCRIRNYVNGLTQGAGIVGFSRGPLSLLSQLHIRKFSYCLSPNGT 240
           LG ANQ+ STPDIAFGC I+N+V GLTQGAGIVGF+RGPLSLLSQLHI+KFSYCLS NGT
Sbjct: 181 LGGANQKVSTPDIAFGCGIKNHVKGLTQGAGIVGFNRGPLSLLSQLHIKKFSYCLSLNGT 240

Query: 241 GCLAFGSSSTSFDNTTNQAVKNTPLIQNPSNPSYYYLSLQGITVGQKFLPVPSSWFELNA 300
           G L FG SSTS DNTTN+A+K TPLIQNP NPSYYYLSLQGITVGQK+LPVPSS F LN 
Sbjct: 241 GSLVFG-SSTSIDNTTNRAIKTTPLIQNPYNPSYYYLSLQGITVGQKYLPVPSSSFRLNT 300

Query: 301 DGSGGVIIDSDTSITYITEDAFDVLKPVFTSQTNLPVINSASIGLDLCFELPSPNNSKLD 360
           DGSGG+IIDS TS+TYITED FDVLKPVF SQTNL VI S SIGLDLCF+LPS NNSKLD
Sbjct: 301 DGSGGMIIDSGTSMTYITEDVFDVLKPVFASQTNLQVIWS-SIGLDLCFQLPSHNNSKLD 360

Query: 361 VPDLIFRFEGLDLKLPVENYMVVNEEVGIVCLAMGAAGGLSIFGSMQHQNMLVLHDLKKK 420
           VPDLIF FE LDLKLPVENYMVVNEE+GIVCLAMGAAGGLSIFGSMQHQNMLVLHDL+KK
Sbjct: 361 VPDLIFHFEDLDLKLPVENYMVVNEELGIVCLAMGAAGGLSIFGSMQHQNMLVLHDLEKK 420

Query: 421 VISFIPTQCSRINY 434
           V+SFIPTQCS+++Y
Sbjct: 421 VVSFIPTQCSQLDY 432

BLAST of Cla97C08G148380 vs. NCBI nr
Match: RVX22508.1 (Aspartic proteinase nepenthesin-1 [Vitis vinifera])

HSP 1 Score: 440.7 bits (1132), Expect = 1.5e-119
Identity = 228/442 (51.58%), Postives = 295/442 (66.74%), Query Frame = 0

Query: 2   PMANCCFLLLLL----LPVLHS--QPAFSESSLKPGFHFDLTHVDATQNFTKFEMLRRGI 61
           P+ +  FLLL+L    LP   S   P F    L+ GF   L H+DA +NFTK ++++RGI
Sbjct: 7   PLPSLPFLLLILTVLGLPRSSSTLMPGFRRQQLETGFQVGLRHIDAGRNFTKLQLIQRGI 66

Query: 62  ERDKTRIQNFKKMTVNFE---VRMPLVDQQGSYLMNLSFGTPPVSFSAIFDTGSDLIWTQ 121
            R + R+Q    M    E    + P+    G +++NL  GTPPV F AI DTGSDLIWTQ
Sbjct: 67  NRGRQRLQRMSGMATTAERNDFQPPVHVGDGEFVVNLMIGTPPVPFPAIMDTGSDLIWTQ 126

Query: 122 CNPCLECFGQPTPVYDPAQSSSFTNATRSAALCQALTNSSSSNGCEYSYGYGDGSFTIGY 181
           CNPC  CF Q TPV++P +SS+F+N + S+ LC+ +  S     CEY Y YGD S T G+
Sbjct: 127 CNPCKLCFQQSTPVFNPKRSSTFSNISCSSKLCKGVKPSKCDKSCEYRYTYGDESSTEGF 186

Query: 182 LAFETLTLGEANQQASTPDIAFGCRIRNYVNGLTQGAGIVGFSRGPLSLLSQLHIRKFSY 241
           +A +T+T GE+ ++ S P I FGC + N   G+ Q AG++G  RG LSL+SQL  +KFSY
Sbjct: 187 MAMDTITFGESPKRVSIPRIGFGCGVNNRATGMDQTAGLLGLGRGVLSLVSQLGTQKFSY 246

Query: 242 CLS---PNGTGCLAFGSSSTSFDNTTNQAVKNTPLIQNPSNPSYYYLSLQGITVGQKFLP 301
           CL+    N T  L FG  S ++ N     +  TPLIQNP  PSYYYLSL+GITVG   LP
Sbjct: 247 CLTSIHENKTSSLLFG--SLAYSNFNPGKIPRTPLIQNPFLPSYYYLSLKGITVGYTLLP 306

Query: 302 VPSSWFELNADGSGGVIIDSDTSITYITEDAFDVLKPVFTSQTNLPVINSASIGLDLCFE 361
           +P   F+L  DGSGG+I+DS T+ITY+ EDAFDVLK  F SQT L V NS++ GLDLCF 
Sbjct: 307 IPEFAFQLGKDGSGGMILDSGTTITYLQEDAFDVLKNAFISQTELQVANSSTTGLDLCFH 366

Query: 362 LPSPNNSKLDVPDLIFRFEGLDLKLPVENYMVVNEEVGIVCLAMGAAGGLSIFGSMQHQN 421
           LP  N +++ VP LIF F+GLDL LPVENYMV + E+G++CLA+ A G LSIFG++Q QN
Sbjct: 367 LPVKNAAEVKVPKLIFHFKGLDLALPVENYMVSDPEMGLICLAIDATGSLSIFGNIQQQN 426

Query: 422 MLVLHDLKKKVISFIPTQCSRI 432
           MLVLHDLKK  +S +PTQC ++
Sbjct: 427 MLVLHDLKKSTLSLVPTQCDKV 446

BLAST of Cla97C08G148380 vs. NCBI nr
Match: XP_011045692.1 (PREDICTED: aspartic proteinase nepenthesin-1-like [Populus euphratica])

HSP 1 Score: 438.7 bits (1127), Expect = 5.7e-119
Identity = 225/440 (51.14%), Postives = 304/440 (69.09%), Query Frame = 0

Query: 3   MANCCFLLLLLLPVLHSQPAFSES-------SLKPGFHFDLTHVDATQNFTKFEMLRRGI 62
           M + CF+L L +  +   PAFS S        ++ GF   L HVD+ +N TK E +R G+
Sbjct: 4   MTSLCFVLALAMFTIFFSPAFSTSRRALEHPEMQKGFRVRLKHVDSGKNLTKLERIRHGV 63

Query: 63  ERDKTRIQNFKKMTV----NFEVRMPLVDQQGSYLMNLSFGTPPVSFSAIFDTGSDLIWT 122
           +R + R+Q  K M +    + E+  P++   G +LM L+ GTPP ++SAI DTGSDLIWT
Sbjct: 64  KRGRNRLQRLKTMALVASSSSEIEAPVLPGNGEFLMKLAIGTPPETYSAILDTGSDLIWT 123

Query: 123 QCNPCLECFGQPTPVYDPAQSSSFTNATRSAALCQALTNSSSSNGCEYSYGYGDGSFTIG 182
           QC PC +CF Q TP++DP +SSSF+  + S+ LC+AL  SS SNGCEY Y YGD S T G
Sbjct: 124 QCKPCTQCFHQSTPIFDPKKSSSFSKLSCSSQLCEALPQSSCSNGCEYLYSYGDYSSTQG 183

Query: 183 YLAFETLTLGEANQQASTPDIAFGCRIRNYVNGLTQGAGIVGFSRGPLSLLSQLHIRKFS 242
            LA ETLT G    +AS P++AFGC   N  +G +QGAG+VG  RGPLSL+SQL   KFS
Sbjct: 184 ILASETLTFG----KASVPNVAFGCGANNEGSGFSQGAGLVGLGRGPLSLVSQLKEPKFS 243

Query: 243 YCLS---PNGTGCLAFGSSSTSFDNTTNQAVKNTPLIQNPSNPSYYYLSLQGITVGQKFL 302
           YCL+      T  L  GS ++   N ++ A+K TPLI++P++PS+YYLSL+GI+VG   L
Sbjct: 244 YCLTSVDDAKTSTLLMGSLASV--NASSSAIKTTPLIRSPAHPSFYYLSLEGISVGDTRL 303

Query: 303 PVPSSWFELNADGSGGVIIDSDTSITYITEDAFDVLKPVFTSQTNLPVINSASIGLDLCF 362
           P+  S F L  DGSGG+IIDS T+ITY+ E AF+++   FTSQ NLPV +S S GLD+CF
Sbjct: 304 PIKKSTFSLQDDGSGGLIIDSGTTITYLEERAFNLVAKEFTSQINLPVDSSGSTGLDVCF 363

Query: 363 ELPSPNNSKLDVPDLIFRFEGLDLKLPVENYMVVNEEVGIVCLAMGAAGGLSIFGSMQHQ 422
            LPS  ++ ++VP L+F F+G DL+LP ENYM+ +  +G+ CLAMG++ G+SIFG++Q Q
Sbjct: 364 TLPS-GSTNIEVPKLVFHFDGADLELPAENYMIGDSSMGVACLAMGSSSGMSIFGNVQQQ 423

Query: 423 NMLVLHDLKKKVISFIPTQC 429
           NMLVLHDL+K+ +SF+PTQC
Sbjct: 424 NMLVLHDLEKETLSFLPTQC 436

BLAST of Cla97C08G148380 vs. NCBI nr
Match: XP_034676400.1 (aspartic proteinase nepenthesin-1-like [Vitis riparia])

HSP 1 Score: 438.3 bits (1126), Expect = 7.5e-119
Identity = 226/442 (51.13%), Postives = 294/442 (66.52%), Query Frame = 0

Query: 2   PMANCCFLLLLL----LPVLHS--QPAFSESSLKPGFHFDLTHVDATQNFTKFEMLRRGI 61
           P+ +  FLLL+L    LP   S   P F    L+ GF   L H+DA +NFTK ++++RGI
Sbjct: 7   PLPSLPFLLLILTVLGLPRSSSTLMPGFRRQQLETGFQVGLRHIDAGRNFTKLQLIQRGI 66

Query: 62  ERDKTRIQNFKKMTVNFE---VRMPLVDQQGSYLMNLSFGTPPVSFSAIFDTGSDLIWTQ 121
           +R + R+Q    M    E    + P+    G +++NL  GTPPV F AI DTGSDLIWTQ
Sbjct: 67  DRGRQRLQRMSGMATTAERNDFQAPVHAGDGEFVVNLMIGTPPVPFPAIMDTGSDLIWTQ 126

Query: 122 CNPCLECFGQPTPVYDPAQSSSFTNATRSAALCQALTNSSSSNGCEYSYGYGDGSFTIGY 181
           CNPC  CF Q TPV++P +SS+F+N + S+ LC+ +  S     CEY Y YGD S T G+
Sbjct: 127 CNPCKLCFQQSTPVFNPKRSSTFSNISCSSKLCEGVKPSKCDKSCEYRYTYGDESSTEGF 186

Query: 182 LAFETLTLGEANQQASTPDIAFGCRIRNYVNGLTQGAGIVGFSRGPLSLLSQLHIRKFSY 241
           +A +T+T GE  ++ S P I FGC + N   G+ Q AG++G  RG LSL+SQL  +KFSY
Sbjct: 187 MAMDTITFGELPKRVSIPRIGFGCGVNNRATGMDQTAGLLGLGRGVLSLVSQLSTQKFSY 246

Query: 242 CLS---PNGTGCLAFGSSSTSFDNTTNQAVKNTPLIQNPSNPSYYYLSLQGITVGQKFLP 301
           CL+    N T  L FG  S ++ N     +  TPLIQNP  PSYYYLSL+GITVG   LP
Sbjct: 247 CLTSIHENKTSSLLFG--SLAYSNFNPGKIPRTPLIQNPFLPSYYYLSLKGITVGYTLLP 306

Query: 302 VPSSWFELNADGSGGVIIDSDTSITYITEDAFDVLKPVFTSQTNLPVINSASIGLDLCFE 361
           +P   F+L  DGSGG+I+DS T+ITY+ EDAFDVLK  F SQT L V NS++ GLDLCF 
Sbjct: 307 IPDFAFQLGKDGSGGMILDSGTTITYLQEDAFDVLKNAFISQTELQVANSSTTGLDLCFH 366

Query: 362 LPSPNNSKLDVPDLIFRFEGLDLKLPVENYMVVNEEVGIVCLAMGAAGGLSIFGSMQHQN 421
           LP  N +++ VP LIF F+GLDL LPVENYMV + ++G++CLA+ A G  SIFG++Q QN
Sbjct: 367 LPVKNAAEVKVPKLIFHFKGLDLALPVENYMVSDPDMGLICLAIDATGSPSIFGNIQQQN 426

Query: 422 MLVLHDLKKKVISFIPTQCSRI 432
           MLVLHDLKK  +S +PTQC ++
Sbjct: 427 MLVLHDLKKSTLSLVPTQCDKV 446

BLAST of Cla97C08G148380 vs. NCBI nr
Match: XP_010664223.1 (PREDICTED: aspartic proteinase nepenthesin-1 [Vitis vinifera])

HSP 1 Score: 438.0 bits (1125), Expect = 9.8e-119
Identity = 226/442 (51.13%), Postives = 294/442 (66.52%), Query Frame = 0

Query: 2   PMANCCFLLLLL----LPVLHS--QPAFSESSLKPGFHFDLTHVDATQNFTKFEMLRRGI 61
           P+ +  FLLL+L    LP   S   P F    L+ GF   L H+DA +NFT+ ++++RGI
Sbjct: 7   PLPSLPFLLLILTVLGLPRSSSTLMPGFRRQQLETGFQVGLRHIDAGRNFTRLQLIQRGI 66

Query: 62  ERDKTRIQNFKKMTVNFE---VRMPLVDQQGSYLMNLSFGTPPVSFSAIFDTGSDLIWTQ 121
            R + R+Q    M    E    + P+    G +++NL  GTPPV F AI DTGSDLIWTQ
Sbjct: 67  NRGRQRLQRMSGMATTAERNGFQAPVHVGDGEFVVNLMIGTPPVPFPAIMDTGSDLIWTQ 126

Query: 122 CNPCLECFGQPTPVYDPAQSSSFTNATRSAALCQALTNSSSSNGCEYSYGYGDGSFTIGY 181
           CNPC  CF Q TPV++P +SS+F+N + S+ LC+ +  S     CEY Y YGD S T G+
Sbjct: 127 CNPCKLCFQQSTPVFNPKRSSTFSNISCSSKLCKGVKPSKCDKSCEYRYTYGDESSTEGF 186

Query: 182 LAFETLTLGEANQQASTPDIAFGCRIRNYVNGLTQGAGIVGFSRGPLSLLSQLHIRKFSY 241
           +A +T+T GE  ++ S P I FGC + N   G+ Q AG++G  RG LSL+SQL  +KFSY
Sbjct: 187 MAMDTITFGELPKRVSIPRIGFGCGVNNRATGMDQTAGLLGLGRGVLSLVSQLGTQKFSY 246

Query: 242 CLS---PNGTGCLAFGSSSTSFDNTTNQAVKNTPLIQNPSNPSYYYLSLQGITVGQKFLP 301
           CL+    N T  L FG  S ++ N     +  TPLIQNP  PSYYYL+L+GITVG   LP
Sbjct: 247 CLTSIHENKTSSLLFG--SLAYSNFNPGKIPRTPLIQNPFLPSYYYLALKGITVGYTLLP 306

Query: 302 VPSSWFELNADGSGGVIIDSDTSITYITEDAFDVLKPVFTSQTNLPVINSASIGLDLCFE 361
           +P   F+L  DGSGG+I+DS T+ITY+ EDAFDVLK  F SQT L V NS++ GLDLCF 
Sbjct: 307 IPEFAFQLGKDGSGGMILDSGTTITYLQEDAFDVLKNAFISQTELQVANSSTTGLDLCFH 366

Query: 362 LPSPNNSKLDVPDLIFRFEGLDLKLPVENYMVVNEEVGIVCLAMGAAGGLSIFGSMQHQN 421
           LP  N +++ VP LIF F+GLDL LPVENYMV + E+G++CLA+ A G LSIFG++Q QN
Sbjct: 367 LPVKNAAEVKVPKLIFHFKGLDLALPVENYMVSDPEMGLICLAIDATGSLSIFGNIQQQN 426

Query: 422 MLVLHDLKKKVISFIPTQCSRI 432
           MLVLHDLKK  +S +PTQC ++
Sbjct: 427 MLVLHDLKKSTLSLVPTQCDKV 446

BLAST of Cla97C08G148380 vs. ExPASy Swiss-Prot
Match: Q766C3 (Aspartic proteinase nepenthesin-1 OS=Nepenthes gracilis OX=150966 GN=nep1 PE=1 SV=1)

HSP 1 Score: 355.1 bits (910), Expect = 1.1e-96
Identity = 200/437 (45.77%), Postives = 268/437 (61.33%), Query Frame = 0

Query: 8   FLLLLLLPVLHSQPAFSES---------SLKPGFHFDLTHVDATQNFTKFEMLRRGIERD 67
           FLL L +  +   P  S S         +   GF   L HVD+ +N TKF++L R IER 
Sbjct: 8   FLLALSIVYIFVAPTHSTSRTALNHRHEAKVTGFQIMLEHVDSGKNLTKFQLLERAIERG 67

Query: 68  KTRIQNFKKMTVN-FEVRMPLVDQQGSYLMNLSFGTPPVSFSAIFDTGSDLIWTQCNPCL 127
             R+Q  + M      V   +    G YLMNLS GTP   FSAI DTGSDLIWTQC PC 
Sbjct: 68  SRRLQRLEAMLNGPSGVETSVYAGDGEYLMNLSIGTPAQPFSAIMDTGSDLIWTQCQPCT 127

Query: 128 ECFGQPTPVYDPAQSSSFTNATRSAALCQALTNSSSSNG-CEYSYGYGDGSFTIGYLAFE 187
           +CF Q TP+++P  SSSF+    S+ LCQAL++ + SN  C+Y+YGYGDGS T G +  E
Sbjct: 128 QCFNQSTPIFNPQGSSSFSTLPCSSQLCQALSSPTCSNNFCQYTYGYGDGSETQGSMGTE 187

Query: 188 TLTLGEANQQASTPDIAFGCRIRNYVNGLTQGAGIVGFSRGPLSLLSQLHIRKFSYCLSP 247
           TLT G      S P+I FGC   N   G   GAG+VG  RGPLSL SQL + KFSYC++P
Sbjct: 188 TLTFG----SVSIPNITFGCGENNQGFGQGNGAGLVGMGRGPLSLPSQLDVTKFSYCMTP 247

Query: 248 NGTGC---LAFGSSSTSFDNTTNQAVKNTPLIQNPSNPSYYYLSLQGITVGQKFLPVPSS 307
            G+     L  GS +    N+      NT LIQ+   P++YY++L G++VG   LP+  S
Sbjct: 248 IGSSTPSNLLLGSLA----NSVTAGSPNTTLIQSSQIPTFYYITLNGLSVGSTRLPIDPS 307

Query: 308 WFELNA-DGSGGVIIDSDTSITYITEDAFDVLKPVFTSQTNLPVINSASIGLDLCFELPS 367
            F LN+ +G+GG+IIDS T++TY   +A+  ++  F SQ NLPV+N +S G DLCF+ PS
Sbjct: 308 AFALNSNNGTGGIIIDSGTTLTYFVNNAYQSVRQEFISQINLPVVNGSSSGFDLCFQTPS 367

Query: 368 PNNSKLDVPDLIFRFEGLDLKLPVENYMVVNEEVGIVCLAMGAAG-GLSIFGSMQHQNML 427
            + S L +P  +  F+G DL+LP ENY  ++   G++CLAMG++  G+SIFG++Q QNML
Sbjct: 368 -DPSNLQIPTFVMHFDGGDLELPSENYF-ISPSNGLICLAMGSSSQGMSIFGNIQQQNML 427

Query: 428 VLHDLKKKVISFIPTQC 429
           V++D    V+SF   QC
Sbjct: 428 VVYDTGNSVVSFASAQC 434

BLAST of Cla97C08G148380 vs. ExPASy Swiss-Prot
Match: Q766C2 (Aspartic proteinase nepenthesin-2 OS=Nepenthes gracilis OX=150966 GN=nep2 PE=1 SV=1)

HSP 1 Score: 350.1 bits (897), Expect = 3.5e-95
Identity = 182/407 (44.72%), Postives = 266/407 (65.36%), Query Frame = 0

Query: 29  KPGFHFDLTHVDATQNFTKFEMLRRGIERDKTRIQNFKKMTVNFE-VRMPLVDQQGSYLM 88
           +PG   DL  VD+ +N TK+E+++R I+R + R+++   M  +   +  P+    G YLM
Sbjct: 39  QPGLRVDLEQVDSGKNLTKYELIKRAIKRGERRMRSINAMLQSSSGIETPVYAGDGEYLM 98

Query: 89  NLSFGTPPVSFSAIFDTGSDLIWTQCNPCLECFGQPTPVYDPAQSSSFTNATRSAALCQA 148
           N++ GTP  SFSAI DTGSDLIWTQC PC +CF QPTP+++P  SSSF+     +  CQ 
Sbjct: 99  NVAIGTPDSSFSAIMDTGSDLIWTQCEPCTQCFSQPTPIFNPQDSSSFSTLPCESQYCQD 158

Query: 149 L-TNSSSSNGCEYSYGYGDGSFTIGYLAFETLTLGEANQQASTPDIAFGCRIRNYVNGLT 208
           L + + ++N C+Y+YGYGDGS T GY+A ET T     + +S P+IAFGC   N   G  
Sbjct: 159 LPSETCNNNECQYTYGYGDGSTTQGYMATETFTF----ETSSVPNIAFGCGEDNQGFGQG 218

Query: 209 QGAGIVGFSRGPLSLLSQLHIRKFSYCLSPNGT---GCLAFGSSSTSFDNTTNQAVKNTP 268
            GAG++G   GPLSL SQL + +FSYC++  G+     LA GS+++       +   +T 
Sbjct: 219 NGAGLIGMGWGPLSLPSQLGVGQFSYCMTSYGSSSPSTLALGSAASG----VPEGSPSTT 278

Query: 269 LIQNPSNPSYYYLSLQGITVGQKFLPVPSSWFELNADGSGGVIIDSDTSITYITEDAFDV 328
           LI +  NP+YYY++LQGITVG   L +PSS F+L  DG+GG+IIDS T++TY+ +DA++ 
Sbjct: 279 LIHSSLNPTYYYITLQGITVGGDNLGIPSSTFQLQDDGTGGMIIDSGTTLTYLPQDAYNA 338

Query: 329 LKPVFTSQTNLPVINSASIGLDLCFELPSPNNSKLDVPDLIFRFEGLDLKLPVENYMVVN 388
           +   FT Q NLP ++ +S GL  CF+ PS + S + VP++  +F+G  L L  +N ++  
Sbjct: 339 VAQAFTDQINLPTVDESSSGLSTCFQQPS-DGSTVQVPEISMQFDGGVLNLGEQNILISP 398

Query: 389 EEVGIVCLAMGAAG--GLSIFGSMQHQNMLVLHDLKKKVISFIPTQC 429
            E G++CLAMG++   G+SIFG++Q Q   VL+DL+   +SF+PTQC
Sbjct: 399 AE-GVICLAMGSSSQLGISIFGNIQQQETQVLYDLQNLAVSFVPTQC 435

BLAST of Cla97C08G148380 vs. ExPASy Swiss-Prot
Match: Q6XBF8 (Aspartic proteinase CDR1 OS=Arabidopsis thaliana OX=3702 GN=CDR1 PE=1 SV=1)

HSP 1 Score: 237.3 bits (604), Expect = 3.3e-61
Identity = 156/445 (35.06%), Postives = 229/445 (51.46%), Query Frame = 0

Query: 8   FLLLLLLPVLHSQPAFSESSLKP--GFHFDLTHVDATQN------FTKFEMLRRGIERDK 67
           F  +LL   L S    S ++ KP  GF  DL H D+ ++       T  + LR  I R  
Sbjct: 5   FSSVLLSLCLLSSLFLSNANAKPKLGFTADLIHRDSPKSPFYNPMETSSQRLRNAIHRSV 64

Query: 68  TRIQNFKKMTVNFEVRMPLVDQQGSYLMNLSFGTPPVSFSAIFDTGSDLIWTQCNPCLEC 127
            R+ +F +     + ++ L    G YLMN+S GTPP    AI DTGSDL+WTQC PC +C
Sbjct: 65  NRVFHFTEKDNTPQPQIDLTSNSGEYLMNVSIGTPPFPIMAIADTGSDLLWTQCAPCDDC 124

Query: 128 FGQPTPVYDPAQSSSFTNATRSAALCQALTN----SSSSNGCEYSYGYGDGSFTIGYLAF 187
           + Q  P++DP  SS++ + + S++ C AL N    S++ N C YS  YGD S+T G +A 
Sbjct: 125 YTQVDPLFDPKTSSTYKDVSCSSSQCTALENQASCSTNDNTCSYSLSYGDNSYTKGNIAV 184

Query: 188 ETLTLGEAN-QQASTPDIAFGCRIRNYVNGLTQGAGIVGFSRGPLSLLSQLHIR---KFS 247
           +TLTLG ++ +     +I  GC   N      +G+GIVG   GP+SL+ QL      KFS
Sbjct: 185 DTLTLGSSDTRPMQLKNIIIGCGHNNAGTFNKKGSGIVGLGGGPVSLIKQLGDSIDGKFS 244

Query: 248 YCLSP-----NGTGCLAFGSSSTSFDNTTNQAVKNTPLIQNPSNPSYYYLSLQGITVGQK 307
           YCL P     + T  + FG+++      +   V +TPLI   S  ++YYL+L+ I+VG K
Sbjct: 245 YCLVPLTSKKDQTSKINFGTNAI----VSGSGVVSTPLIAKASQETFYYLTLKSISVGSK 304

Query: 308 FLPVPSSWFELNADGSGGVIIDSDTSITYITEDAFDVLKPVFTSQTNLPVINSASIGLDL 367
            +    S  E      G +IIDS T++T +  + +  L+    S  +         GL L
Sbjct: 305 QIQYSGSDSE---SSEGNIIIDSGTTLTLLPTEFYSELEDAVASSIDAEKKQDPQSGLSL 364

Query: 368 CFELPSPNNSKLDVPDLIFRFEGLDLKLPVENYMVVNEEVGIVCLAMGAAGGLSIFGSMQ 427
           C+         L VP +   F+G D+KL   N  V   E  +VC A   +   SI+G++ 
Sbjct: 365 CYSA----TGDLKVPVITMHFDGADVKLDSSNAFVQVSE-DLVCFAFRGSPSFSIYGNVA 424

Query: 428 HQNMLVLHDLKKKVISFIPTQCSRI 432
             N LV +D   K +SF PT C+++
Sbjct: 425 QMNFLVGYDTVSKTVSFKPTDCAKM 437

BLAST of Cla97C08G148380 vs. ExPASy Swiss-Prot
Match: Q7XV21 (Aspartyl protease 37 OS=Oryza sativa subsp. japonica OX=39947 GN=AP37 PE=3 SV=2)

HSP 1 Score: 226.1 bits (575), Expect = 7.6e-58
Identity = 157/471 (33.33%), Postives = 234/471 (49.68%), Query Frame = 0

Query: 10  LLLLLPVLHSQPAFSESSLKP-GFHFDLTHVDAT----QNFTKFEMLRRGIERDKTRIQN 69
           +LLLL  L + PA   S   P  F  +L  VDA+     N T+ E+LRR I+R + R+  
Sbjct: 5   VLLLLLALAALPA---SCAPPRSFRLELASVDASAADAANLTEHELLRRAIQRSRYRLAG 64

Query: 70  F----------KKMTVNFEVRMPLVDQQGSYLMNLSFGTPPVSFSAIFDTGSDLIWTQCN 129
                      +K  V      P++   G YL+ L  GTPP  F+A  DT SDLIWTQC 
Sbjct: 65  IGMARGEAASARKAVV---AETPIMPAGGEYLVKLGIGTPPYKFTAAIDTASDLIWTQCQ 124

Query: 130 PCLECFGQPTPVYDPAQSSSFTNATRSAALCQAL----TNSSSSNGCEYSYGYGDGSFTI 189
           PC  C+ Q  P+++P  SS++     S+  C  L            C+Y+Y Y   + T 
Sbjct: 125 PCTGCYHQVDPMFNPRVSSTYAALPCSSDTCDELDVHRCGHDDDESCQYTYTYSGNATTE 184

Query: 190 GYLAFETLTLGEANQQASTPDIAFGCRIRNYVNG-LTQGAGIVGFSRGPLSLLSQLHIRK 249
           G LA + L +GE     +   +AFGC   +       Q +G+VG  RGPLSL+SQL +R+
Sbjct: 185 GTLAVDKLVIGE----DAFRGVAFGCSTSSTGGAPPPQASGVVGLGRGPLSLVSQLSVRR 244

Query: 250 FSYCLSPNGT---GCLAFGSSSTSFDNTTNQAVKNTPLIQNPSNPSYYYLSLQGITVGQK 309
           F+YCL P  +   G L  G+ + +  N TN+     P+ ++P  PSYYYL+L G+ +G +
Sbjct: 245 FAYCLPPPASRIPGKLVLGADADAARNATNRIA--VPMRRDPRYPSYYYLNLDGLLIGDR 304

Query: 310 FL--------------------PVPSSWFELNADGSG---GVIIDSDTSITYITEDAFDV 369
            +                    P PS      A G     G+IID  ++IT++    +D 
Sbjct: 305 AMSLPPTTTTTATATATAPAPAPTPSPNATAVAVGDANRYGMIIDIASTITFLEASLYDE 364

Query: 370 LKPVFTSQTNLPVINSASIGLDLCFELP-SPNNSKLDVPDLIFRFEGLDLKLPVENYMVV 429
           L      +  LP    +S+GLDLCF LP      ++ VP +   F+G  L+L        
Sbjct: 365 LVNDLEVEIRLPRGTGSSLGLDLCFILPDGVAFDRVYVPAVALAFDGRWLRLDKARLFAE 424

Query: 430 NEEVGIVCLAMG--AAGGLSIFGSMQHQNMLVLHDLKKKVISFIPTQCSRI 432
           + E G++CL +G   AG +SI G+ Q QNM VL++L++  ++F+ + C  +
Sbjct: 425 DRESGMMCLMVGRAEAGSVSILGNFQQQNMQVLYNLRRGRVTFVQSPCGAL 463

BLAST of Cla97C08G148380 vs. ExPASy Swiss-Prot
Match: Q3EBM5 (Probable aspartic protease At2g35615 OS=Arabidopsis thaliana OX=3702 GN=At2g35615 PE=3 SV=1)

HSP 1 Score: 214.2 bits (544), Expect = 3.0e-54
Identity = 142/446 (31.84%), Postives = 230/446 (51.57%), Query Frame = 0

Query: 11  LLLLPVLHSQPAFSESSLKPGFHFDLTHVDA------TQNFTKFEMLRRGIERDKTRIQN 70
           +LL   L      S S     F  +L H D+          T  + L     R  +R + 
Sbjct: 5   ILLCFFLFFSVTLSSSGHPKNFSVELIHRDSPLSPIYNPQITVTDRLNAAFLRSVSRSRR 64

Query: 71  FKKMTVNFEVRMPLVDQQGSYLMNLSFGTPPVSFSAIFDTGSDLIWTQCNPCLECFGQPT 130
           F       +++  L+   G + M+++ GTPP+   AI DTGSDL W QC PC +C+ +  
Sbjct: 65  FNHQLSQTDLQSGLIGADGEFFMSITIGTPPIKVFAIADTGSDLTWVQCKPCQQCYKENG 124

Query: 131 PVYDPAQSSSFTNATRSAALCQALTNS-----SSSNGCEYSYGYGDGSFTIGYLAFETLT 190
           P++D  +SS++ +    +  CQAL+++      S+N C+Y Y YGD SF+ G +A ET++
Sbjct: 125 PIFDKKKSSTYKSEPCDSRNCQALSSTERGCDESNNICKYRYSYGDQSFSKGDVATETVS 184

Query: 191 LGEAN-QQASTPDIAFGCRIRNYVNGLTQGAGIVGFSRGPLSLLSQLH---IRKFSYCLS 250
           +  A+    S P   FGC   N       G+GI+G   G LSL+SQL     +KFSYCLS
Sbjct: 185 IDSASGSPVSFPGTVFGCGYNNGGTFDETGSGIIGLGGGHLSLISQLGSSISKKFSYCLS 244

Query: 251 -----PNGTGCLAFGSSSTSFDNTTNQAVKNTPLIQNPSNPSYYYLSLQGITVGQKFLPV 310
                 NGT  +  G++S     + +  V +TPL+ +    +YYYL+L+ I+VG+K +P 
Sbjct: 245 HKSATTNGTSVINLGTNSIPSSLSKDSGVVSTPLV-DKEPLTYYYLTLEAISVGKKKIPY 304

Query: 311 PSSWFELNADG-----SGGVIIDSDTSITYITEDAFDVL-KPVFTSQTNLPVINSASIGL 370
             S +  N DG     SG +IIDS T++T +    FD     V  S T    ++     L
Sbjct: 305 TGSSYNPNDDGILSETSGNIIIDSGTTLTLLEAGFFDKFSSAVEESVTGAKRVSDPQGLL 364

Query: 371 DLCFELPSPNNSKLDVPDLIFRFEGLDLKL-PVENYMVVNEEVGIVCLAMGAAGGLSIFG 430
             CF+     ++++ +P++   F G D++L P+  ++ ++E+  +VCL+M     ++I+G
Sbjct: 365 SHCFK---SGSAEIGLPEITVHFTGADVRLSPINAFVKLSED--MVCLSMVPTTEVAIYG 424

BLAST of Cla97C08G148380 vs. ExPASy TrEMBL
Match: A0A2N9HF56 (Peptidase A1 domain-containing protein OS=Fagus sylvatica OX=28930 GN=FSB_LOCUS40859 PE=3 SV=1)

HSP 1 Score: 441.4 bits (1134), Expect = 4.3e-120
Identity = 238/438 (54.34%), Postives = 304/438 (69.41%), Query Frame = 0

Query: 8   FLLLLLLPVL-----HSQPAFSESSLKPGFHFDLTHVDA-TQNFTKFEMLRRG-IERDKT 67
           F LLLL+ +L      S   F ES  K GF   LT VDA  +N T+ ++++R  I R KT
Sbjct: 10  FSLLLLVVILSLLSFSSGLPFDESPEKTGFRVSLTRVDAGKKNLTELQLVQRAIINRVKT 69

Query: 68  RIQNFKKMTVNF---EVRMPLVDQQGSYLMNLSFGTPPVSFSAIFDTGSDLIWTQCNPCL 127
           R+     +T N    +++ P+    G +LM+LS GTP V F AI DTGSDLIWTQC PC 
Sbjct: 70  RLDRLSAITKNAPNDDIQAPIYVGDGEFLMHLSIGTPAVPFLAIMDTGSDLIWTQCKPCS 129

Query: 128 ECFGQPTPVYDPAQSSSFTNATRSAALCQALTNSSSSNGCEYSYGYGDGSFTIGYLAFET 187
            CF Q TPV++PA SSSF+N T S+ LC+AL+NS   NGCEY   YGD S + G+LA ET
Sbjct: 130 NCFNQSTPVFEPALSSSFSNVTCSSELCKALSNSRCGNGCEYLNRYGDESSSEGFLAMET 189

Query: 188 LTLGEANQQASTPDIAFGCRIRNYVNGLTQGAGIVGFSRGPLSLLSQLHIRKFSYCLS-- 247
           LT GE+ Q+ S P+I FGC + N   G  + AG+VG  RGPLSL+SQL  +KFSYCL+  
Sbjct: 190 LTFGESAQRVSVPNIGFGCGVDNKGKGWNKAAGLVGLGRGPLSLVSQLGSKKFSYCLTSI 249

Query: 248 -PNGTGCLAFGSSSTSFDNTTNQAV-KNTPLIQNPSNPSYYYLSLQGITVGQKFLPVPSS 307
             N +  L FGS +    N +N AV K TPLI+NP  PS+YYLSL+GITVGQ  LP+P S
Sbjct: 250 GDNKSSSLLFGSLADL--NLSNGAVLKTTPLIRNPILPSFYYLSLEGITVGQTLLPIPKS 309

Query: 308 WFELNADGSGGVIIDSDTSITYITEDAFDVLKPVFTSQTNLPVINSASIGLDLCFELPSP 367
            F+L +DG GGVI+DS T+IT + EDAFDVLK VF +QT L V N  + GLD+CF+LPS 
Sbjct: 310 LFQLGSDGGGGVILDSGTTITSLHEDAFDVLKQVFKAQTKLRVSNLYTRGLDVCFDLPSQ 369

Query: 368 NNSKLDVPDLIFRFEGLDLKLPVENYMVVNEEVGIVCLAMGAAGGLSIFGSMQHQNMLVL 427
           +   + VP L+F FEGLDL+LPVENYM+ + ++G+VCLAMGA G LSIFG++Q QNMLVL
Sbjct: 370 DVKDIKVPKLVFHFEGLDLELPVENYMIADTKLGVVCLAMGATGRLSIFGNIQQQNMLVL 429

Query: 428 HDLKKKVISFIPTQCSRI 432
           HDL+K+ +SFIPT+C ++
Sbjct: 430 HDLEKETVSFIPTKCDQL 445

BLAST of Cla97C08G148380 vs. ExPASy TrEMBL
Match: A0A438KMS9 (Aspartic proteinase nepenthesin-1 OS=Vitis vinifera OX=29760 GN=nep1_0 PE=3 SV=1)

HSP 1 Score: 440.7 bits (1132), Expect = 7.3e-120
Identity = 228/442 (51.58%), Postives = 295/442 (66.74%), Query Frame = 0

Query: 2   PMANCCFLLLLL----LPVLHS--QPAFSESSLKPGFHFDLTHVDATQNFTKFEMLRRGI 61
           P+ +  FLLL+L    LP   S   P F    L+ GF   L H+DA +NFTK ++++RGI
Sbjct: 7   PLPSLPFLLLILTVLGLPRSSSTLMPGFRRQQLETGFQVGLRHIDAGRNFTKLQLIQRGI 66

Query: 62  ERDKTRIQNFKKMTVNFE---VRMPLVDQQGSYLMNLSFGTPPVSFSAIFDTGSDLIWTQ 121
            R + R+Q    M    E    + P+    G +++NL  GTPPV F AI DTGSDLIWTQ
Sbjct: 67  NRGRQRLQRMSGMATTAERNDFQPPVHVGDGEFVVNLMIGTPPVPFPAIMDTGSDLIWTQ 126

Query: 122 CNPCLECFGQPTPVYDPAQSSSFTNATRSAALCQALTNSSSSNGCEYSYGYGDGSFTIGY 181
           CNPC  CF Q TPV++P +SS+F+N + S+ LC+ +  S     CEY Y YGD S T G+
Sbjct: 127 CNPCKLCFQQSTPVFNPKRSSTFSNISCSSKLCKGVKPSKCDKSCEYRYTYGDESSTEGF 186

Query: 182 LAFETLTLGEANQQASTPDIAFGCRIRNYVNGLTQGAGIVGFSRGPLSLLSQLHIRKFSY 241
           +A +T+T GE+ ++ S P I FGC + N   G+ Q AG++G  RG LSL+SQL  +KFSY
Sbjct: 187 MAMDTITFGESPKRVSIPRIGFGCGVNNRATGMDQTAGLLGLGRGVLSLVSQLGTQKFSY 246

Query: 242 CLS---PNGTGCLAFGSSSTSFDNTTNQAVKNTPLIQNPSNPSYYYLSLQGITVGQKFLP 301
           CL+    N T  L FG  S ++ N     +  TPLIQNP  PSYYYLSL+GITVG   LP
Sbjct: 247 CLTSIHENKTSSLLFG--SLAYSNFNPGKIPRTPLIQNPFLPSYYYLSLKGITVGYTLLP 306

Query: 302 VPSSWFELNADGSGGVIIDSDTSITYITEDAFDVLKPVFTSQTNLPVINSASIGLDLCFE 361
           +P   F+L  DGSGG+I+DS T+ITY+ EDAFDVLK  F SQT L V NS++ GLDLCF 
Sbjct: 307 IPEFAFQLGKDGSGGMILDSGTTITYLQEDAFDVLKNAFISQTELQVANSSTTGLDLCFH 366

Query: 362 LPSPNNSKLDVPDLIFRFEGLDLKLPVENYMVVNEEVGIVCLAMGAAGGLSIFGSMQHQN 421
           LP  N +++ VP LIF F+GLDL LPVENYMV + E+G++CLA+ A G LSIFG++Q QN
Sbjct: 367 LPVKNAAEVKVPKLIFHFKGLDLALPVENYMVSDPEMGLICLAIDATGSLSIFGNIQQQN 426

Query: 422 MLVLHDLKKKVISFIPTQCSRI 432
           MLVLHDLKK  +S +PTQC ++
Sbjct: 427 MLVLHDLKKSTLSLVPTQCDKV 446

BLAST of Cla97C08G148380 vs. ExPASy TrEMBL
Match: F6H0S5 (Peptidase A1 domain-containing protein OS=Vitis vinifera OX=29760 GN=VIT_18s0001g03090 PE=3 SV=1)

HSP 1 Score: 438.0 bits (1125), Expect = 4.7e-119
Identity = 226/442 (51.13%), Postives = 294/442 (66.52%), Query Frame = 0

Query: 2   PMANCCFLLLLL----LPVLHS--QPAFSESSLKPGFHFDLTHVDATQNFTKFEMLRRGI 61
           P+ +  FLLL+L    LP   S   P F    L+ GF   L H+DA +NFT+ ++++RGI
Sbjct: 7   PLPSLPFLLLILTVLGLPRSSSTLMPGFRRQQLETGFQVGLRHIDAGRNFTRLQLIQRGI 66

Query: 62  ERDKTRIQNFKKMTVNFE---VRMPLVDQQGSYLMNLSFGTPPVSFSAIFDTGSDLIWTQ 121
            R + R+Q    M    E    + P+    G +++NL  GTPPV F AI DTGSDLIWTQ
Sbjct: 67  NRGRQRLQRMSGMATTAERNGFQAPVHVGDGEFVVNLMIGTPPVPFPAIMDTGSDLIWTQ 126

Query: 122 CNPCLECFGQPTPVYDPAQSSSFTNATRSAALCQALTNSSSSNGCEYSYGYGDGSFTIGY 181
           CNPC  CF Q TPV++P +SS+F+N + S+ LC+ +  S     CEY Y YGD S T G+
Sbjct: 127 CNPCKLCFQQSTPVFNPKRSSTFSNISCSSKLCKGVKPSKCDKSCEYRYTYGDESSTEGF 186

Query: 182 LAFETLTLGEANQQASTPDIAFGCRIRNYVNGLTQGAGIVGFSRGPLSLLSQLHIRKFSY 241
           +A +T+T GE  ++ S P I FGC + N   G+ Q AG++G  RG LSL+SQL  +KFSY
Sbjct: 187 MAMDTITFGELPKRVSIPRIGFGCGVNNRATGMDQTAGLLGLGRGVLSLVSQLGTQKFSY 246

Query: 242 CLS---PNGTGCLAFGSSSTSFDNTTNQAVKNTPLIQNPSNPSYYYLSLQGITVGQKFLP 301
           CL+    N T  L FG  S ++ N     +  TPLIQNP  PSYYYL+L+GITVG   LP
Sbjct: 247 CLTSIHENKTSSLLFG--SLAYSNFNPGKIPRTPLIQNPFLPSYYYLALKGITVGYTLLP 306

Query: 302 VPSSWFELNADGSGGVIIDSDTSITYITEDAFDVLKPVFTSQTNLPVINSASIGLDLCFE 361
           +P   F+L  DGSGG+I+DS T+ITY+ EDAFDVLK  F SQT L V NS++ GLDLCF 
Sbjct: 307 IPEFAFQLGKDGSGGMILDSGTTITYLQEDAFDVLKNAFISQTELQVANSSTTGLDLCFH 366

Query: 362 LPSPNNSKLDVPDLIFRFEGLDLKLPVENYMVVNEEVGIVCLAMGAAGGLSIFGSMQHQN 421
           LP  N +++ VP LIF F+GLDL LPVENYMV + E+G++CLA+ A G LSIFG++Q QN
Sbjct: 367 LPVKNAAEVKVPKLIFHFKGLDLALPVENYMVSDPEMGLICLAIDATGSLSIFGNIQQQN 426

Query: 422 MLVLHDLKKKVISFIPTQCSRI 432
           MLVLHDLKK  +S +PTQC ++
Sbjct: 427 MLVLHDLKKSTLSLVPTQCDKV 446

BLAST of Cla97C08G148380 vs. ExPASy TrEMBL
Match: A0A4U5QCA8 (Aspartyl protease family protein OS=Populus alba OX=43335 GN=D5086_0000107380 PE=3 SV=1)

HSP 1 Score: 436.0 bits (1120), Expect = 1.8e-118
Identity = 224/441 (50.79%), Postives = 300/441 (68.03%), Query Frame = 0

Query: 3   MANCCFLLLLLLPVLHSQPAFSESS--------LKPGFHFDLTHVDATQNFTKFEMLRRG 62
           M + CF+L L +  +   PAFS S         L+ GF   L HVD+ +N TK E +R G
Sbjct: 4   MTSLCFVLALAMFTIFFSPAFSTSRRALEHPKILQKGFRVRLKHVDSGKNLTKLERIRHG 63

Query: 63  IERDKTRIQNFKKMTV----NFEVRMPLVDQQGSYLMNLSFGTPPVSFSAIFDTGSDLIW 122
           ++R + R+Q  K M +    + E+  P++   G +LM L+ GTPP ++SAI DTGSDLIW
Sbjct: 64  VKRGRNRLQRLKAMALVASSSSEIEAPVLPGNGEFLMKLAIGTPPETYSAILDTGSDLIW 123

Query: 123 TQCNPCLECFGQPTPVYDPAQSSSFTNATRSAALCQALTNSSSSNGCEYSYGYGDGSFTI 182
           TQC PC +CF Q TP++DP +SSSF+  + S+ LC+AL  SS + GCEY Y YGD S T 
Sbjct: 124 TQCKPCTQCFDQSTPIFDPKKSSSFSKLSCSSQLCEALPQSSCNKGCEYQYSYGDYSSTQ 183

Query: 183 GYLAFETLTLGEANQQASTPDIAFGCRIRNYVNGLTQGAGIVGFSRGPLSLLSQLHIRKF 242
           G LA ETLT G    +AS P++AFGC   N  +G +QGAG+VG  RGPLSL+SQL   KF
Sbjct: 184 GILASETLTFG----KASVPNVAFGCGADNEGSGFSQGAGLVGLGRGPLSLVSQLKEPKF 243

Query: 243 SYCLS---PNGTGCLAFGSSSTSFDNTTNQAVKNTPLIQNPSNPSYYYLSLQGITVGQKF 302
           SYCL+      T  L  GS ++   N ++ A+K TPLI +P+ PS+YYLSL+GI+VG   
Sbjct: 244 SYCLTTVDDTKTSTLLMGSLASV--NASSSAIKTTPLIHSPAQPSFYYLSLEGISVGDTR 303

Query: 303 LPVPSSWFELNADGSGGVIIDSDTSITYITEDAFDVLKPVFTSQTNLPVINSASIGLDLC 362
           LP+  S F L  DGSGG+IIDS T+ITY+ E AFD++   FTSQ NLPV +S S GLD+C
Sbjct: 304 LPIKKSTFSLQDDGSGGLIIDSGTTITYLEESAFDLVAKEFTSQINLPVDSSGSTGLDVC 363

Query: 363 FELPSPNNSKLDVPDLIFRFEGLDLKLPVENYMVVNEEVGIVCLAMGAAGGLSIFGSMQH 422
           F LPS  ++ ++VP L+F F+G DL+LP ENYM+ +  +G+ CLAMG++ G+SIFG++Q 
Sbjct: 364 FTLPS-GSTNMEVPKLVFHFDGADLELPAENYMIGDSSMGVACLAMGSSSGMSIFGNVQQ 423

Query: 423 QNMLVLHDLKKKVISFIPTQC 429
           QNMLVLHDL+K+ +SF+PT C
Sbjct: 424 QNMLVLHDLEKETLSFLPTPC 437

BLAST of Cla97C08G148380 vs. ExPASy TrEMBL
Match: B9GH97 (Peptidase A1 domain-containing protein OS=Populus trichocarpa OX=3694 GN=POPTR_001G306200 PE=3 SV=2)

HSP 1 Score: 433.0 bits (1112), Expect = 1.5e-117
Identity = 221/440 (50.23%), Postives = 302/440 (68.64%), Query Frame = 0

Query: 3   MANCCFLLLLLLPVLHSQPAFSES-------SLKPGFHFDLTHVDATQNFTKFEMLRRGI 62
           M + CF+L L +  +   PAFS S        ++ GF   L HVD+ +N TK E +R G+
Sbjct: 4   MTSLCFVLALAMFTIFFSPAFSTSRRALEHPKMQKGFRVRLKHVDSGKNLTKLERIRHGV 63

Query: 63  ERDKTRIQNFKKMTV----NFEVRMPLVDQQGSYLMNLSFGTPPVSFSAIFDTGSDLIWT 122
           +R + R+Q  + M +    + E+  P++   G +LM L+ GTPP ++SAI DTGSDLIWT
Sbjct: 64  KRGRNRLQRLQAMALVASSSSEIEAPVLPGNGEFLMKLAIGTPPETYSAILDTGSDLIWT 123

Query: 123 QCNPCLECFGQPTPVYDPAQSSSFTNATRSAALCQALTNSSSSNGCEYSYGYGDGSFTIG 182
           QC PC +CF Q TP++DP +SSSF+  + S+ LC+AL  SS +NGCEY Y YGD S T G
Sbjct: 124 QCKPCTQCFHQSTPIFDPKKSSSFSKLSCSSQLCEALPQSSCNNGCEYLYSYGDYSSTQG 183

Query: 183 YLAFETLTLGEANQQASTPDIAFGCRIRNYVNGLTQGAGIVGFSRGPLSLLSQLHIRKFS 242
            LA ETLT G    +AS P +AFGC   N  +G +QGAG+VG  RGPLSL+SQL   KFS
Sbjct: 184 ILASETLTFG----KASVPHVAFGCGADNEGSGFSQGAGLVGLGRGPLSLVSQLKEPKFS 243

Query: 243 YCLS---PNGTGCLAFGSSSTSFDNTTNQAVKNTPLIQNPSNPSYYYLSLQGITVGQKFL 302
           YCL+      T  L  GS ++   N ++ A+K TPLI +P++PS+YYLSL+GI+VG   L
Sbjct: 244 YCLTTVDDTKTSTLLMGSLASV--NASSSAIKTTPLIHSPAHPSFYYLSLEGISVGDTRL 303

Query: 303 PVPSSWFELNADGSGGVIIDSDTSITYITEDAFDVLKPVFTSQTNLPVINSASIGLDLCF 362
           P+  S F L  DGSGG+IIDS T+ITY+ E AF+++   FT++ NLPV +S S GLD+CF
Sbjct: 304 PIKKSTFSLQDDGSGGLIIDSGTTITYLEESAFNLVAKEFTAKINLPVDSSGSTGLDVCF 363

Query: 363 ELPSPNNSKLDVPDLIFRFEGLDLKLPVENYMVVNEEVGIVCLAMGAAGGLSIFGSMQHQ 422
            LPS  ++ ++VP L+F F+G DL+LP ENYM+ +  +G+ CLAMG++ G+SIFG++Q Q
Sbjct: 364 TLPS-GSTNIEVPKLVFHFDGADLELPAENYMIGDSSMGVACLAMGSSSGMSIFGNVQQQ 423

Query: 423 NMLVLHDLKKKVISFIPTQC 429
           NMLVLHDL+K+ +SF+PTQC
Sbjct: 424 NMLVLHDLEKETLSFLPTQC 436

BLAST of Cla97C08G148380 vs. TAIR 10
Match: AT2G03200.1 (Eukaryotic aspartyl protease family protein )

HSP 1 Score: 380.6 bits (976), Expect = 1.7e-105
Identity = 210/452 (46.46%), Postives = 279/452 (61.73%), Query Frame = 0

Query: 8   FLLLL--LLPVLHSQPAFSESSL-----KPGFHFDLTHVDATQNFTKFEMLRRGIERDKT 67
           FL+L   L+ V  S+ +  + +L     + GF   L HVD+ +N TK + ++RGI R   
Sbjct: 14  FLILFSCLISVSSSRRSLIDRTLPKNLPRSGFRLSLRHVDSGKNLTKIQKIQRGINRGFH 73

Query: 68  RIQNFKKMTV---------NFEVRMPLVDQQGSYLMNLSFGTPPVSFSAIFDTGSDLIWT 127
           R+     + V            ++ P     G +LM LS G P V +SAI DTGSDLIWT
Sbjct: 74  RLNRLGAVAVLAVASKPDDTNNIKAPTHGGSGEFLMELSIGNPAVKYSAIVDTGSDLIWT 133

Query: 128 QCNPCLECFGQPTPVYDPAQSSSFTNATRSAALCQALTNSS---SSNGCEYSYGYGDGSF 187
           QC PC ECF QPTP++DP +SSS++    S+ LC AL  S+     + CEY Y YGD S 
Sbjct: 134 QCKPCTECFDQPTPIFDPEKSSSYSKVGCSSGLCNALPRSNCNEDKDACEYLYTYGDYSS 193

Query: 188 TIGYLAFETLTLGEANQQASTPDIAFGCRIRNYVNGLTQGAGIVGFSRGPLSLLSQLHIR 247
           T G LA ET T  + N   S   I FGC + N  +G +QG+G+VG  RGPLSL+SQL   
Sbjct: 194 TRGLLATETFTFEDEN---SISGIGFGCGVENEGDGFSQGSGLVGLGRGPLSLISQLKET 253

Query: 248 KFSYCLS----PNGTGCLAFGSSSTSFDNTT-----NQAVKNTPLIQNPSNPSYYYLSLQ 307
           KFSYCL+       +  L  GS ++   N T      +  K   L++NP  PS+YYL LQ
Sbjct: 254 KFSYCLTSIEDSEASSSLFIGSLASGIVNKTGASLDGEVTKTMSLLRNPDQPSFYYLELQ 313

Query: 308 GITVGQKFLPVPSSWFELNADGSGGVIIDSDTSITYITEDAFDVLKPVFTSQTNLPVINS 367
           GITVG K L V  S FEL  DG+GG+IIDS T+ITY+ E AF VLK  FTS+ +LPV +S
Sbjct: 314 GITVGAKRLSVEKSTFELAEDGTGGMIIDSGTTITYLEETAFKVLKEEFTSRMSLPVDDS 373

Query: 368 ASIGLDLCFELPSPNNSKLDVPDLIFRFEGLDLKLPVENYMVVNEEVGIVCLAMGAAGGL 427
            S GLDLCF+LP    + + VP +IF F+G DL+LP ENYMV +   G++CLAMG++ G+
Sbjct: 374 GSTGLDLCFKLPDAAKN-IAVPKMIFHFKGADLELPGENYMVADSSTGVLCLAMGSSNGM 433

Query: 428 SIFGSMQHQNMLVLHDLKKKVISFIPTQCSRI 432
           SIFG++Q QN  VLHDL+K+ +SF+PT+C ++
Sbjct: 434 SIFGNVQQQNFNVLHDLEKETVSFVPTECGKL 461

BLAST of Cla97C08G148380 vs. TAIR 10
Match: AT5G33340.1 (Eukaryotic aspartyl protease family protein )

HSP 1 Score: 237.3 bits (604), Expect = 2.4e-62
Identity = 156/445 (35.06%), Postives = 229/445 (51.46%), Query Frame = 0

Query: 8   FLLLLLLPVLHSQPAFSESSLKP--GFHFDLTHVDATQN------FTKFEMLRRGIERDK 67
           F  +LL   L S    S ++ KP  GF  DL H D+ ++       T  + LR  I R  
Sbjct: 5   FSSVLLSLCLLSSLFLSNANAKPKLGFTADLIHRDSPKSPFYNPMETSSQRLRNAIHRSV 64

Query: 68  TRIQNFKKMTVNFEVRMPLVDQQGSYLMNLSFGTPPVSFSAIFDTGSDLIWTQCNPCLEC 127
            R+ +F +     + ++ L    G YLMN+S GTPP    AI DTGSDL+WTQC PC +C
Sbjct: 65  NRVFHFTEKDNTPQPQIDLTSNSGEYLMNVSIGTPPFPIMAIADTGSDLLWTQCAPCDDC 124

Query: 128 FGQPTPVYDPAQSSSFTNATRSAALCQALTN----SSSSNGCEYSYGYGDGSFTIGYLAF 187
           + Q  P++DP  SS++ + + S++ C AL N    S++ N C YS  YGD S+T G +A 
Sbjct: 125 YTQVDPLFDPKTSSTYKDVSCSSSQCTALENQASCSTNDNTCSYSLSYGDNSYTKGNIAV 184

Query: 188 ETLTLGEAN-QQASTPDIAFGCRIRNYVNGLTQGAGIVGFSRGPLSLLSQLHIR---KFS 247
           +TLTLG ++ +     +I  GC   N      +G+GIVG   GP+SL+ QL      KFS
Sbjct: 185 DTLTLGSSDTRPMQLKNIIIGCGHNNAGTFNKKGSGIVGLGGGPVSLIKQLGDSIDGKFS 244

Query: 248 YCLSP-----NGTGCLAFGSSSTSFDNTTNQAVKNTPLIQNPSNPSYYYLSLQGITVGQK 307
           YCL P     + T  + FG+++      +   V +TPLI   S  ++YYL+L+ I+VG K
Sbjct: 245 YCLVPLTSKKDQTSKINFGTNAI----VSGSGVVSTPLIAKASQETFYYLTLKSISVGSK 304

Query: 308 FLPVPSSWFELNADGSGGVIIDSDTSITYITEDAFDVLKPVFTSQTNLPVINSASIGLDL 367
            +    S  E      G +IIDS T++T +  + +  L+    S  +         GL L
Sbjct: 305 QIQYSGSDSE---SSEGNIIIDSGTTLTLLPTEFYSELEDAVASSIDAEKKQDPQSGLSL 364

Query: 368 CFELPSPNNSKLDVPDLIFRFEGLDLKLPVENYMVVNEEVGIVCLAMGAAGGLSIFGSMQ 427
           C+         L VP +   F+G D+KL   N  V   E  +VC A   +   SI+G++ 
Sbjct: 365 CYSA----TGDLKVPVITMHFDGADVKLDSSNAFVQVSE-DLVCFAFRGSPSFSIYGNVA 424

Query: 428 HQNMLVLHDLKKKVISFIPTQCSRI 432
             N LV +D   K +SF PT C+++
Sbjct: 425 QMNFLVGYDTVSKTVSFKPTDCAKM 437

BLAST of Cla97C08G148380 vs. TAIR 10
Match: AT1G64830.1 (Eukaryotic aspartyl protease family protein )

HSP 1 Score: 218.8 bits (556), Expect = 8.7e-57
Identity = 149/447 (33.33%), Postives = 239/447 (53.47%), Query Frame = 0

Query: 3   MANCCFLLLLLLPVLHSQPAFSESSLKPGFHFDLTHVDATQNFTKFEMLRRGIERDKTRI 62
           MA+  F  LL L +L +  A+     K GF  DL H D+ ++   +       +R +  I
Sbjct: 1   MASLIFATLLSLLLLSNVNAYP----KDGFTIDLIHRDSPKS-PFYNSAETSSQRMRNAI 60

Query: 63  QNFKKMTVNF--------EVRMPLVDQQGSYLMNLSFGTPPVSFSAIFDTGSDLIWTQCN 122
           +   + T+ F          +  +   +G YLMN+S GTPPV   AI DTGSDLIWTQCN
Sbjct: 61  RRSARSTLQFSNDDASPNSPQSFITSNRGEYLMNISIGTPPVPILAIADTGSDLIWTQCN 120

Query: 123 PCLECFGQPTPVYDPAQSSSFTNATRSAALCQALTNSSSS---NGCEYSYGYGDGSFTIG 182
           PC +C+ Q +P++DP +SS++   + S++ C+AL ++S S   N C Y+  YGD S+T G
Sbjct: 121 PCEDCYQQTSPLFDPKESSTYRKVSCSSSQCRALEDASCSTDENTCSYTITYGDNSYTKG 180

Query: 183 YLAFETLTLGEANQQ-ASTPDIAFGCRIRNYVNGLTQGAGIVGFSRGPLSLLSQLHIR-- 242
            +A +T+T+G + ++  S  ++  GC   N       G+GI+G   G  SL+SQL     
Sbjct: 181 DVAVDTVTMGSSGRRPVSLRNMIIGCGHENTGTFDPAGSGIIGLGGGSTSLVSQLRKSIN 240

Query: 243 -KFSYCLSP--NGTGCLAFGSSSTSFDNTTNQAVKNTPLIQNPSNPSYYYLSLQGITVGQ 302
            KFSYCL P  + TG  +  +  T+   + +  V  + + ++P+  +YY+L+L+ I+VG 
Sbjct: 241 GKFSYCLVPFTSETGLTSKINFGTNGIVSGDGVVSTSMVKKDPA--TYYFLNLEAISVGS 300

Query: 303 KFLPVPSSWFELNADGSGGVIIDSDTSITYITEDAFDVLKPVFTSQTNLPVINSASIGLD 362
           K +   S+ F     G G ++IDS T++T +  + +  L+ V  S      +      L 
Sbjct: 301 KKIQFTSTIF---GTGEGNIVIDSGTTLTLLPSNFYYELESVVASTIKAERVQDPDGILS 360

Query: 363 LCFELPSPNNSKLDVPDLIFRFEGLDLKL-PVENYMVVNEEVGIVCLAMGAAGGLSIFGS 422
           LC+     ++S   VPD+   F+G D+KL  +  ++ V+E+V   C A  A   L+IFG+
Sbjct: 361 LCYR----DSSSFKVPDITVHFKGGDVKLGNLNTFVAVSEDVS--CFAFAANEQLTIFGN 420

Query: 423 MQHQNMLVLHDLKKKVISFIPTQCSRI 432
           +   N LV +D     +SF  T CS++
Sbjct: 421 LAQMNFLVGYDTVSGTVSFKKTDCSQM 431

BLAST of Cla97C08G148380 vs. TAIR 10
Match: AT1G31450.1 (Eukaryotic aspartyl protease family protein )

HSP 1 Score: 215.7 bits (548), Expect = 7.3e-56
Identity = 134/387 (34.63%), Postives = 205/387 (52.97%), Query Frame = 0

Query: 62  IQNFKKMTVNFEVRMPLVDQQGSYLMNLSFGTPPVSFSAIFDTGSDLIWTQCNPCLECFG 121
           I   ++ T   +++  L+   G Y M++S GTPP    AI DTGSDL W QC PC +C+ 
Sbjct: 62  ISRSRRFTTKTDLQSGLISNGGEYFMSISIGTPPSKVFAIADTGSDLTWVQCKPCQQCYK 121

Query: 122 QPTPVYDPAQSSSFTNATRSAALCQALTN-----SSSSNGCEYSYGYGDGSFTIGYLAFE 181
           Q +P++D  +SS++   +  +  CQAL+        S + C+Y Y YGD SFT G +A E
Sbjct: 122 QNSPLFDKKKSSTYKTESCDSKTCQALSEHEEGCDESKDICKYRYSYGDNSFTKGDVATE 181

Query: 182 TLTL-GEANQQASTPDIAFGCRIRNYVNGLTQGAGIVGFSRGPLSLLSQLHI---RKFSY 241
           T+++   +    S P   FGC   N       G+GI+G   GPLSL+SQL     +KFSY
Sbjct: 182 TISIDSSSGSSVSFPGTVFGCGYNNGGTFEETGSGIIGLGGGPLSLVSQLGSSIGKKFSY 241

Query: 242 CLS-----PNGTGCLAFGSSSTSFDNTTNQAVKNTPLIQNPSNPSYYYLSLQGITVGQKF 301
           CLS      NGT  +  G++S   + + + A   TPLIQ     +YY+L+L+ +TVG+  
Sbjct: 242 CLSHTAATTNGTSVINLGTNSIPSNPSKDSATLTTPLIQKDPE-TYYFLTLEAVTVGKTK 301

Query: 302 LPVPSSWFELNADGS---GGVIIDSDTSITYITEDAFDVL-KPVFTSQTNLPVINSASIG 361
           LP     + LN   S   G +IIDS T++T +    +D     V  S T    ++     
Sbjct: 302 LPYTGGGYGLNGKSSKRTGNIIIDSGTTLTLLDSGFYDDFGTAVEESVTGAKRVSDPQGL 361

Query: 362 LDLCFELPSPNNSKLDVPDLIFRFEGLDLKL-PVENYMVVNEEVGIVCLAMGAAGGLSIF 421
           L  CF+     + ++ +P +   F   D+KL P+  ++ +NE+   VCL+M     ++I+
Sbjct: 362 LTHCFK---SGDKEIGLPAITMHFTNADVKLSPINAFVKLNEDT--VCLSMIPTTEVAIY 421

Query: 422 GSMQHQNMLVLHDLKKKVISFIPTQCS 430
           G+M   + LV +DL+ K +SF    CS
Sbjct: 422 GNMVQMDFLVGYDLETKTVSFQRMDCS 442

BLAST of Cla97C08G148380 vs. TAIR 10
Match: AT3G61820.1 (Eukaryotic aspartyl protease family protein )

HSP 1 Score: 214.5 bits (545), Expect = 1.6e-55
Identity = 155/442 (35.07%), Postives = 215/442 (48.64%), Query Frame = 0

Query: 22  AFSESSLKPGFHFDLTHVDATQNF---TKFEMLRRGIERDKTRIQNFKKMTV-----NFE 81
           + SES+     H  L+HVDA  +F   +  ++    ++RD  R+++   +       N  
Sbjct: 53  SLSESTTSLSVH--LSHVDALSSFSDASPADLFNLRLQRDSLRVKSITSLAAVSTGRNAT 112

Query: 82  VRMP-------------LVDQQGSYLMNLSFGTPPVSFSAIFDTGSDLIWTQCNPCLECF 141
            R P             L    G Y M L  GTP  +   + DTGSD++W QC+PC  C+
Sbjct: 113 KRTPRTAGGFSGAVISGLSQGSGEYFMRLGVGTPATNVYMVLDTGSDVVWLQCSPCKACY 172

Query: 142 GQPTPVYDPAQSSSFTNATRSAALCQALTNSSS-----SNGCEYSYGYGDGSFTIGYLAF 201
            Q   ++DP +S +F      + LC+ L +SS      S  C Y   YGDGSFT G  + 
Sbjct: 173 NQTDAIFDPKKSKTFATVPCGSRLCRRLDDSSECVTRRSKTCLYQVSYGDGSFTEGDFST 232

Query: 202 ETLTLGEANQQASTPDIAFGCRIRNYVNGLTQG-AGIVGFSRGPLSLLSQLHIR---KFS 261
           ETLT       A    +  GC   N   GL  G AG++G  RG LS  SQ   R   KFS
Sbjct: 233 ETLTF----HGARVDHVPLGCGHDN--EGLFVGAAGLLGLGRGGLSFPSQTKNRYNGKFS 292

Query: 262 YCL-SPNGTGCLAFGSSSTSFDN-TTNQAVKNTPLIQNPSNPSYYYLSLQGITVGQKFLP 321
           YCL     +G  +   S+  F N    +    TPL+ NP   ++YYL L GI+VG   +P
Sbjct: 293 YCLVDRTSSGSSSKPPSTIVFGNAAVPKTSVFTPLLTNPKLDTFYYLQLLGISVGGSRVP 352

Query: 322 -VPSSWFELNADGSGGVIIDSDTSITYITEDAFDVLKPVF-TSQTNLPVINSASIGLDLC 381
            V  S F+L+A G+GGVIIDS TS+T +T+ A+  L+  F    T L    S S+  D C
Sbjct: 353 GVSESQFKLDATGNGGVIIDSGTSVTRLTQPAYVALRDAFRLGATKLKRAPSYSL-FDTC 412

Query: 382 FELPSPNNSKLDVPDLIFRFEGLDLKLPVENYMVVNEEVGIVCLAM-GAAGGLSIFGSMQ 429
           F+L      K  VP ++F F G ++ LP  NY++     G  C A  G  G LSI G++Q
Sbjct: 413 FDLSGMTTVK--VPTVVFHFGGGEVSLPASNYLIPVNTEGRFCFAFAGTMGSLSIIGNIQ 472

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
XP_038886455.16.8e-20583.41aspartic proteinase nepenthesin-2-like [Benincasa hispida][more]
RVX22508.11.5e-11951.58Aspartic proteinase nepenthesin-1 [Vitis vinifera][more]
XP_011045692.15.7e-11951.14PREDICTED: aspartic proteinase nepenthesin-1-like [Populus euphratica][more]
XP_034676400.17.5e-11951.13aspartic proteinase nepenthesin-1-like [Vitis riparia][more]
XP_010664223.19.8e-11951.13PREDICTED: aspartic proteinase nepenthesin-1 [Vitis vinifera][more]
Match NameE-valueIdentityDescription
Q766C31.1e-9645.77Aspartic proteinase nepenthesin-1 OS=Nepenthes gracilis OX=150966 GN=nep1 PE=1 S... [more]
Q766C23.5e-9544.72Aspartic proteinase nepenthesin-2 OS=Nepenthes gracilis OX=150966 GN=nep2 PE=1 S... [more]
Q6XBF83.3e-6135.06Aspartic proteinase CDR1 OS=Arabidopsis thaliana OX=3702 GN=CDR1 PE=1 SV=1[more]
Q7XV217.6e-5833.33Aspartyl protease 37 OS=Oryza sativa subsp. japonica OX=39947 GN=AP37 PE=3 SV=2[more]
Q3EBM53.0e-5431.84Probable aspartic protease At2g35615 OS=Arabidopsis thaliana OX=3702 GN=At2g3561... [more]
Match NameE-valueIdentityDescription
A0A2N9HF564.3e-12054.34Peptidase A1 domain-containing protein OS=Fagus sylvatica OX=28930 GN=FSB_LOCUS4... [more]
A0A438KMS97.3e-12051.58Aspartic proteinase nepenthesin-1 OS=Vitis vinifera OX=29760 GN=nep1_0 PE=3 SV=1[more]
F6H0S54.7e-11951.13Peptidase A1 domain-containing protein OS=Vitis vinifera OX=29760 GN=VIT_18s0001... [more]
A0A4U5QCA81.8e-11850.79Aspartyl protease family protein OS=Populus alba OX=43335 GN=D5086_0000107380 PE... [more]
B9GH971.5e-11750.23Peptidase A1 domain-containing protein OS=Populus trichocarpa OX=3694 GN=POPTR_0... [more]
Match NameE-valueIdentityDescription
AT2G03200.11.7e-10546.46Eukaryotic aspartyl protease family protein [more]
AT5G33340.12.4e-6235.06Eukaryotic aspartyl protease family protein [more]
AT1G64830.18.7e-5733.33Eukaryotic aspartyl protease family protein [more]
AT1G31450.17.3e-5634.63Eukaryotic aspartyl protease family protein [more]
AT3G61820.11.6e-5535.07Eukaryotic aspartyl protease family protein [more]
InterPro
Analysis Name: InterPro Annotations of Watermelon (97103) v2.5
Date Performed: 2022-01-31
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR021109Aspartic peptidase domain superfamilyGENE3D2.40.70.10Acid Proteasescoord: 59..246
e-value: 2.7E-48
score: 166.5
IPR021109Aspartic peptidase domain superfamilyGENE3D2.40.70.10Acid Proteasescoord: 247..433
e-value: 1.2E-48
score: 167.3
IPR021109Aspartic peptidase domain superfamilySUPERFAMILY50630Acid proteasescoord: 80..430
IPR032799Xylanase inhibitor, C-terminalPFAMPF14541TAXi_Ccoord: 274..423
e-value: 2.2E-27
score: 95.8
IPR032861Xylanase inhibitor, N-terminalPFAMPF14543TAXi_Ncoord: 85..246
e-value: 3.6E-44
score: 151.1
NoneNo IPR availablePANTHERPTHR47967OS07G0603500 PROTEIN-RELATEDcoord: 16..429
NoneNo IPR availablePANTHERPTHR47967:SF23OS08G0469000 PROTEINcoord: 16..429
IPR033121Peptidase family A1 domainPROSITEPS51767PEPTIDASE_A1coord: 85..424
score: 36.854156
IPR034161Pepsin-like domain, plantCDDcd05476pepsin_A_like_plantcoord: 85..428
e-value: 2.9871E-93
score: 280.303

Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
Cla97C08G148380.2Cla97C08G148380.2mRNA


GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
cellular_component GO:0005576 extracellular region
molecular_function GO:0016787 hydrolase activity