Cla97C08G147405 (gene) Watermelon (97103) v2.5

Overview


Name	Cla97C08G147405
Type	gene
Organism	Citrullus lanatus subsp. vulgaris cv. 97103 (Watermelon (97103) v2.5)
Description	rRNA N-glycosidase
Location	Cla97Chr08: 12099555 .. 12100422 (-)
RNA-Seq Expression	Cla97C08G147405
Synteny	Cla97C08G147405

Sequences

The following sequences are available for this feature:

Gene sequence (with intron)

Legend: polypeptideexonCDS

Hold the cursor over a type above to highlight its positions in the sequence below.

ATGAGACTCTTAGGATTTTACTTCCTTCTTGCACTGTATGTTGGCGGCTGTGTTGTTGAAGCCGATGTAAGCTTCTCTATGTTGGGCGCCACAAGTGAAACCTACAAACAGTTCATAAAGAATCTGCGAAGTGCACTCACAGTTGGATCTCCGACAGTCTACAACATCCCCGTCTTGAAGAGCACAGCAACGGGGCCAGCAGGCTTTCTATTAGTTCACCTCACAAATTACAATGATGAATCCATCACGGTGGCAATAGACGTAGTGAACGTGTACGTGGTAGCATATGGAGCTGGAAACAATGCCTATTTCTTGAATGACGCTTCAACAGAAGCCAACAACCTTCTATTTAAAGGCCTCAAACATACAAGACTTCCTTACAACAGAAGCCAACAACCTTCTATTTAAAGGCCTCAAACATACAAGACTTCCTTACAAAGGCAATTACGATGGCCTTGAGACTGCTGCAGGAAAGATTTCAAGAGAGAAAATTGAACTTGGATTTTCTGAGATAAGCAGTGCCATTGGCAACATGTTCCACCACAATGCTGGTACTTCTGTCCCTAAAGCCTTCATTGTCATTATCGAAACTGTGTCTGAAGCTGCAAGATATAAATATATTGAGCAGAGAGTTTCTGAGAATGTGAAGACAAAGTTCTTGCCGGACCCTGCTTTCTTAAGCTTAGAGAATCGTTGGTCTGATTTATCCCAACAAGTTCAAATCGCACAGACGCGCGGAGGCCAGTTTGCTCGTGCTGTTGAGCTACGAACTGTTGGCAATAAACCAGTGCTCGTAACCAATGTTAGTTCGCCCATTGTCAAAGGAATTGCACTTCTGTTGTACTATAAACCCAGTGTTGCCGCCTAA

mRNA sequence

ATGAGACTCTTAGGATTTTACTTCCTTCTTGCACTGTATGTTGGCGGCTGTGTTGTTGAAGCCGATGTAAGCTTCTCTATGTTGGGCGCCACAAGTGAAACCTACAAACAGTTCATAAAGAATCTGCGAAGTGCACTCACAGTTGGATCTCCGACAGTCTACAACATCCCCGTCTTGAAGAGCACAGCAACGGGGCCAGCAGGCTTTCTATTAGTTCACCTCACAAATTACAATGATGAATCCATCACGGTGGCAATAGACAAGCCAACAACCTTCTATTTAAAGGCCTCAAACATACAAGACTTCCTTACAACAGAAGCCAACAACCTTCTATTTAAAGGCCTCAAACATACAAGACTTCCTTACAAAGGCAATTACGATGGCCTTGAGACTGCTGCAGGAAAGATTTCAAGAGAGAAAATTGAACTTGGATTTTCTGAGATAAGCAGTGCCATTGGCAACATGTTCCACCACAATGCTGGTACTTCTGTCCCTAAAGCCTTCATTGTCATTATCGAAACTGTGTCTGAAGCTGCAAGATATAAATATATTGAGCAGAGAGTTTCTGAGAATGTGAAGACAAAGTTCTTGCCGGACCCTGCTTTCTTAAGCTTAGAGAATCGTTGGTCTGATTTATCCCAACAAGTTCAAATCGCACAGACGCGCGGAGGCCAGTTTGCTCGTGCTGTTGAGCTACGAACTGTTGGCAATAAACCAGTGCTCGTAACCAATGTTAGTTCGCCCATTGTCAAAGGAATTGCACTTCTGTTGTACTATAAACCCAGTGTTGCCGCCTAA

Coding sequence (CDS)

ATGAGACTCTTAGGATTTTACTTCCTTCTTGCACTGTATGTTGGCGGCTGTGTTGTTGAAGCCGATGTAAGCTTCTCTATGTTGGGCGCCACAAGTGAAACCTACAAACAGTTCATAAAGAATCTGCGAAGTGCACTCACAGTTGGATCTCCGACAGTCTACAACATCCCCGTCTTGAAGAGCACAGCAACGGGGCCAGCAGGCTTTCTATTAGTTCACCTCACAAATTACAATGATGAATCCATCACGGTGGCAATAGACAAGCCAACAACCTTCTATTTAAAGGCCTCAAACATACAAGACTTCCTTACAACAGAAGCCAACAACCTTCTATTTAAAGGCCTCAAACATACAAGACTTCCTTACAAAGGCAATTACGATGGCCTTGAGACTGCTGCAGGAAAGATTTCAAGAGAGAAAATTGAACTTGGATTTTCTGAGATAAGCAGTGCCATTGGCAACATGTTCCACCACAATGCTGGTACTTCTGTCCCTAAAGCCTTCATTGTCATTATCGAAACTGTGTCTGAAGCTGCAAGATATAAATATATTGAGCAGAGAGTTTCTGAGAATGTGAAGACAAAGTTCTTGCCGGACCCTGCTTTCTTAAGCTTAGAGAATCGTTGGTCTGATTTATCCCAACAAGTTCAAATCGCACAGACGCGCGGAGGCCAGTTTGCTCGTGCTGTTGAGCTACGAACTGTTGGCAATAAACCAGTGCTCGTAACCAATGTTAGTTCGCCCATTGTCAAAGGAATTGCACTTCTGTTGTACTATAAACCCAGTGTTGCCGCCTAA

Protein sequence

MRLLGFYFLLALYVGGCVVEADVSFSMLGATSETYKQFIKNLRSALTVGSPTVYNIPVLKSTATGPAGFLLVHLTNYNDESITVAIDKPTTFYLKASNIQDFLTTEANNLLFKGLKHTRLPYKGNYDGLETAAGKISREKIELGFSEISSAIGNMFHHNAGTSVPKAFIVIIETVSEAARYKYIEQRVSENVKTKFLPDPAFLSLENRWSDLSQQVQIAQTRGGQFARAVELRTVGNKPVLVTNVSSPIVKGIALLLYYKPSVAA

Homology

[Close]

BLAST of Cla97C08G147405 vs. NCBI nr
Match: XP_022158192.1 (ribosome-inactivating protein bryodin II-like [Momordica charantia])

HSP 1 Score: 389.4 bits (999), Expect = 2.4e-104
Identity = 203/269 (75.46%), Postives = 228/269 (84.76%), Query Frame = 0


Query: 1   MRLLGFYFLLALYVGGCVVEADVSFSMLGATSETYKQFIKNLRSALTVGSPTVYNIPVLK 60
           MR L FYF+LAL  G    EADVSFSMLGATSETY+QFI+NLRS LT+ SP VY IPVL+
Sbjct: 1   MRFLPFYFVLALCFGALAAEADVSFSMLGATSETYRQFIRNLRSTLTIRSPVVYGIPVLQ 60

Query: 61  STATGPAGFLLVHLTNYNDESITVAIDKPTTFYL--KASNIQDFL---TTEANNLLFKGL 120
           STA G A F+LVHLTNYN ESITVAID    + +  +A N   FL   + EANN+LFKG+
Sbjct: 61  STAVGSARFILVHLTNYNTESITVAIDVVNIYVVAYRAGNNAYFLNDASAEANNVLFKGI 120

Query: 121 KHTRLPYKGNYDGLETAAGKISREKIELGFSEISSAIGNMFHHNAGTSVPKAFIVIIETV 180
           KH RLPYKGNYDGLETAAGKISREKI+LGFSEISS+IGNMFHHN GTS+ +AFIVII++V
Sbjct: 121 KHVRLPYKGNYDGLETAAGKISREKIDLGFSEISSSIGNMFHHNVGTSIARAFIVIIQSV 180

Query: 181 SEAARYKYIEQRVSENVKTKFLPDPAFLSLENRWSDLSQQVQIAQTRGGQFARAVELRTV 240
            EAAR+KYIEQRVSENVKTKF PDPAFLSLENRWSDLS+Q+QIAQ R G+FARA+ELRTV
Sbjct: 181 LEAARFKYIEQRVSENVKTKFKPDPAFLSLENRWSDLSEQIQIAQKREGEFARAIELRTV 240

Query: 241 GNKPVLVTNVSSPIVKGIALLLYYKPSVA 265
            NKP++VTNVSSPIVKGIALLLYYK  VA
Sbjct: 241 SNKPIIVTNVSSPIVKGIALLLYYKVRVA 269

[Close]

BLAST of Cla97C08G147405 vs. NCBI nr
Match: XP_022158193.1 (ribosome-inactivating protein bryodin II-like [Momordica charantia])

HSP 1 Score: 384.8 bits (987), Expect = 6.0e-103
Identity = 204/268 (76.12%), Postives = 231/268 (86.19%), Query Frame = 0


Query: 1   MRLLGFYFLLALYVGGCVVEADVSFSMLGATSETYKQFIKNLRSALTVGSPTVYNIPVLK 60
           MRLL FYF LALY+G    EADVSFSMLG+TSETYKQFIKN+RSALTVGS  VY+IPVLK
Sbjct: 1   MRLLPFYFFLALYLGARAAEADVSFSMLGSTSETYKQFIKNVRSALTVGSQIVYDIPVLK 60

Query: 61  STATGPAGFLLVHLTNYNDESITVAIDKPTTFYL--KASNIQDFL---TTEANNLLFKGL 120
           +TATG A FLLVHLTNYN ESI VAID    + +  +A N   FL   + EA+ +LF+G+
Sbjct: 61  ATATGSARFLLVHLTNYNSESIIVAIDVVNVYVVAYRAGNNAYFLNDASAEADRVLFQGI 120

Query: 121 KHTRLPYKGNYDGLETAAGKISREKIELGFSEISSAIGNMFHHNAGTSVPKAFIVIIETV 180
            H +L YKGNYDGLETAA KISREKI+LGFSEISS+IGNMFHHNAGTSVP+AFIVII++V
Sbjct: 121 NHVKLSYKGNYDGLETAA-KISREKIDLGFSEISSSIGNMFHHNAGTSVPRAFIVIIQSV 180

Query: 181 SEAARYKYIEQRVSENVKTKFLPDPAFLSLENRWSDLSQQVQIAQTRGGQFARAVELRTV 240
           SEAAR+KYIEQRVSENVKT F PDPAFLSL+NRWSDLS+Q+QIAQ RGG+FARAVELRTV
Sbjct: 181 SEAARFKYIEQRVSENVKTTFKPDPAFLSLQNRWSDLSEQIQIAQKRGGEFARAVELRTV 240

Query: 241 GNKPVLVTNVSSPIVKGIALLLYYKPSV 264
            NKP+LVT+VSSP+VKGIALLLYYK SV
Sbjct: 241 SNKPILVTDVSSPVVKGIALLLYYKVSV 267

[Close]

BLAST of Cla97C08G147405 vs. NCBI nr
Match: BAG12408.1 (karasurin-H precursor [Trichosanthes kirilowii var. japonica])

HSP 1 Score: 383.3 bits (983), Expect = 1.7e-102
Identity = 199/269 (73.98%), Postives = 229/269 (85.13%), Query Frame = 0


Query: 1   MRLLGFYFLLALYVGGCVVEADVSFSMLGATSETYKQFIKNLRSALTVGSPTVYNIPVLK 60
           M+L+GFY +LALYVG  V EADV+FS+LG+TS TYKQFI+NLR+ALTVGSPTV NIPVLK
Sbjct: 1   MKLVGFYLVLALYVGAQVTEADVNFSLLGSTSATYKQFIQNLRAALTVGSPTVENIPVLK 60

Query: 61  STATGPAGFLLVHLTNYNDESITVAIDKPTTFYL--KASNIQDFL---TTEANNLLFKGL 120
            TA G A FLLVHLTNYN ESITVA+D    + +  +A N   FL   + EANN+LFKG+
Sbjct: 61  GTAAGLARFLLVHLTNYNGESITVAVDVVNVYVVAYRAGNNAYFLNDASAEANNVLFKGI 120

Query: 121 KHTRLPYKGNYDGLETAAGKISREKIELGFSEISSAIGNMFHHNAGTSVPKAFIVIIETV 180
            H RLPY GNYDGLETAAG+ISREKI LGFSEISSAIG+MFH+NAGTSVPKAFIVII+TV
Sbjct: 121 NHVRLPYGGNYDGLETAAGRISREKIYLGFSEISSAIGSMFHYNAGTSVPKAFIVIIQTV 180

Query: 181 SEAARYKYIEQRVSENVKTKFLPDPAFLSLENRWSDLSQQVQIAQTRGGQFARAVELRTV 240
           SEAAR+KYI+QRVSENV+TKFLPDPAFLSLEN W  LS+Q+QIAQ RGG+FAR +ELRTV
Sbjct: 181 SEAARFKYIQQRVSENVQTKFLPDPAFLSLENAWGGLSEQIQIAQYRGGEFARPIELRTV 240

Query: 241 GNKPVLVTNVSSPIVKGIALLLYYKPSVA 265
            N P  VTNV+SP+VKG+ALLLYYK +VA
Sbjct: 241 SNTPTFVTNVNSPVVKGVALLLYYKVNVA 269

[Close]

BLAST of Cla97C08G147405 vs. NCBI nr
Match: P98184.1 (RecName: Full=Ribosome-inactivating protein bryodin II; AltName: Full=BD2; AltName: Full=rRNA N-glycosidase; Flags: Precursor [Bryonia dioica])

HSP 1 Score: 354.0 bits (907), Expect = 1.1e-93
Identity = 185/269 (68.77%), Postives = 218/269 (81.04%), Query Frame = 0


Query: 1   MRLLGFYFLLALYVGGCVVE-ADVSFSMLGATSETYKQFIKNLRSALTVGSPTVYNIPVL 60
           MR +GFY +LALYVG  V E  D++FS++GAT  TYK FI+NLR+ LTVG+P VY+IPVL
Sbjct: 1   MRSIGFYSVLALYVGAHVTEDVDINFSLIGATGATYKTFIRNLRTKLTVGTPRVYDIPVL 60

Query: 61  KSTATGPAGFLLVHLTNYNDESITVAIDKPTTFYL--KASNIQDFL---TTEANNLLFKG 120
           ++ A G A F LV LTNYN ES+TVA+D    + +  +A N   FL   +TEANN+LF G
Sbjct: 61  RNAAAGLARFQLVTLTNYNGESVTVALDVVNVYVVAYRAGNTAYFLADASTEANNVLFAG 120

Query: 121 LKHTRLPYKGNYDGLETAAGKISREKIELGFSEISSAIGNMFHHNAGTSVPKAFIVIIET 180
           + H RLPY GNYDGLETAAG+ISRE IELGFSEISSAIGNMF HN GTSVP+AFIVII+T
Sbjct: 121 INHVRLPYGGNYDGLETAAGRISRENIELGFSEISSAIGNMFRHNPGTSVPRAFIVIIQT 180

Query: 181 VSEAARYKYIEQRVSENVKTKFLPDPAFLSLENRWSDLSQQVQIAQTRGGQFARAVELRT 240
           VSEAAR+KYIEQRVSENV TKF PDPAFLSL+N W  LS+Q+QIAQTRGG+FAR VELRT
Sbjct: 181 VSEAARFKYIEQRVSENVGTKFKPDPAFLSLQNAWGSLSEQIQIAQTRGGEFARPVELRT 240

Query: 241 VGNKPVLVTNVSSPIVKGIALLLYYKPSV 264
           V N P  VTNV+SP+VKGIALLLY++ +V
Sbjct: 241 VSNTPTFVTNVNSPVVKGIALLLYFRVNV 269

[Close]

BLAST of Cla97C08G147405 vs. NCBI nr
Match: XP_022158191.1 (ribosome-inactivating protein bryodin II-like [Momordica charantia])

HSP 1 Score: 326.2 bits (835), Expect = 2.5e-85
Identity = 166/246 (67.48%), Postives = 206/246 (83.74%), Query Frame = 0


Query: 21  ADVSFSMLGATSETYKQFIKNLRSALTVGSPTVYNIPVLKSTATGPAGFLLVHLTNYNDE 80
           ++VSFS+LGAT +TY+QFI+NLR+ALT+ S  VYNIPVL +TA   A F+LVHLTNY +E
Sbjct: 35  SNVSFSLLGATGKTYQQFIQNLRNALTINSKIVYNIPVLAATALSSARFILVHLTNYKNE 94

Query: 81  SITVAIDKPTTFYL--KASNIQDFL---TTEANNLLFKGLKHTRLPYKGNYDGLETAAGK 140
           +ITVAID    + +  +A N   FL   + EA ++LFKG+K   LPYKGNYDGLETAAGK
Sbjct: 95  TITVAIDVVNVYIVAYQAGNKAYFLKDASKEARDVLFKGIKQEILPYKGNYDGLETAAGK 154

Query: 141 ISREKIELGFSEISSAIGNMFHHNAGTSVPKAFIVIIETVSEAARYKYIEQRVSENVKTK 200
           ISREKI+LGFSE+ SAIGNM+H+NAGTSVP+AFIV+I+T+SEAAR+KYIE +VS+NV+TK
Sbjct: 155 ISREKIDLGFSELGSAIGNMYHYNAGTSVPRAFIVMIQTISEAARFKYIETKVSQNVETK 214

Query: 201 FLPDPAFLSLENRWSDLSQQVQIAQTRGGQFARAVELRTVGNKPVLVTNVSSPIVKGIAL 260
           F PDP FLSLENRWSDLS+QVQIAQ R G+FAR +E+R+V NKP+LVTNV S +V+G+AL
Sbjct: 215 FKPDPGFLSLENRWSDLSEQVQIAQNRKGEFARPIEVRSVTNKPILVTNVKSKVVEGMAL 274

Query: 261 LLYYKP 262
           LLY KP
Sbjct: 275 LLYSKP 280

[Close]

BLAST of Cla97C08G147405 vs. ExPASy Swiss-Prot
Match: P98184 (Ribosome-inactivating protein bryodin II OS=Bryonia dioica OX=3652 PE=1 SV=1)

HSP 1 Score: 354.0 bits (907), Expect = 1.5e-96
Identity = 185/269 (68.77%), Postives = 218/269 (81.04%), Query Frame = 0


Query: 1   MRLLGFYFLLALYVGGCVVE-ADVSFSMLGATSETYKQFIKNLRSALTVGSPTVYNIPVL 60
           MR +GFY +LALYVG  V E  D++FS++GAT  TYK FI+NLR+ LTVG+P VY+IPVL
Sbjct: 1   MRSIGFYSVLALYVGAHVTEDVDINFSLIGATGATYKTFIRNLRTKLTVGTPRVYDIPVL 60

Query: 61  KSTATGPAGFLLVHLTNYNDESITVAIDKPTTFYL--KASNIQDFL---TTEANNLLFKG 120
           ++ A G A F LV LTNYN ES+TVA+D    + +  +A N   FL   +TEANN+LF G
Sbjct: 61  RNAAAGLARFQLVTLTNYNGESVTVALDVVNVYVVAYRAGNTAYFLADASTEANNVLFAG 120

Query: 121 LKHTRLPYKGNYDGLETAAGKISREKIELGFSEISSAIGNMFHHNAGTSVPKAFIVIIET 180
           + H RLPY GNYDGLETAAG+ISRE IELGFSEISSAIGNMF HN GTSVP+AFIVII+T
Sbjct: 121 INHVRLPYGGNYDGLETAAGRISRENIELGFSEISSAIGNMFRHNPGTSVPRAFIVIIQT 180

Query: 181 VSEAARYKYIEQRVSENVKTKFLPDPAFLSLENRWSDLSQQVQIAQTRGGQFARAVELRT 240
           VSEAAR+KYIEQRVSENV TKF PDPAFLSL+N W  LS+Q+QIAQTRGG+FAR VELRT
Sbjct: 181 VSEAARFKYIEQRVSENVGTKFKPDPAFLSLQNAWGSLSEQIQIAQTRGGEFARPVELRT 240

Query: 241 VGNKPVLVTNVSSPIVKGIALLLYYKPSV 264
           V N P  VTNV+SP+VKGIALLLY++ +V
Sbjct: 241 VSNTPTFVTNVNSPVVKGIALLLYFRVNV 269

[Close]

BLAST of Cla97C08G147405 vs. ExPASy Swiss-Prot
Match: Q00465 (Ribosome-inactivating protein luffin-alpha OS=Luffa aegyptiaca OX=3670 PE=1 SV=1)

HSP 1 Score: 196.4 bits (498), Expect = 4.0e-49
Identity = 119/264 (45.08%), Postives = 172/264 (65.15%), Query Frame = 0


Query: 9   LLALYVGGCVVEADVSFSMLGATSETYKQFIKNLRSALTVGSPTVYNIPVLKSTATGPAG 68
           +LA++V    VEADV FS+ G++S +Y +FI +LR AL   + TVYNI +L S+A+G + 
Sbjct: 8   ILAIFVAASTVEADVRFSLSGSSSTSYSKFIGDLRKALP-SNGTVYNITLLLSSASGASR 67

Query: 69  FLLVHLTNYNDESITVAIDKPTTF---YLKASNIQDFLTTE---ANNLLFKGLKHTRLPY 128
           + L+ L+NY+ ++ITVA+D    +   YL  S    F  ++   A+  +FKG     LPY
Sbjct: 68  YTLMTLSNYDGKAITVAVDVTNVYIMGYLVNSTSYFFNESDAKLASQYVFKGSTIVTLPY 127

Query: 129 KGNYDGLETAAGKISREKIELGFSEISSAIGNMFHHNAGTSVPKAFIVIIETVSEAARYK 188
            GNY+ L+TAAGKI REKI LGF  + SAI  +FH+++ T+   AF+VII+T +EA+R+K
Sbjct: 128 SGNYEKLQTAAGKI-REKIPLGFPALDSAITTLFHYDS-TAAAAAFLVIIQTTAEASRFK 187

Query: 189 YIEQRVSENVKTKFLPDPAFLSLENRWSDLSQQVQIAQTRGGQFARAVELRTVGNKPVLV 248
           YIE ++ E +    +P  A +SLEN WS LS+Q+Q+AQT  G F   V +     + V +
Sbjct: 188 YIEGQIIERISKNQVPSLATISLENEWSALSKQIQLAQTNNGTFKTPVVITDDKGQRVEI 247

Query: 249 TNVSSPIV-KGIALLLYYKPSVAA 266
           TNV+S +V K I LLL YK +VAA
Sbjct: 248 TNVTSKVVTKNIQLLLNYKQNVAA 268

[Close]

BLAST of Cla97C08G147405 vs. ExPASy Swiss-Prot
Match: P56626 (Type I ribosome-inactivating protein trichoanguina OS=Trichosanthes anguina OX=50544 GN=TCA PE=1 SV=2)

HSP 1 Score: 193.7 bits (491), Expect = 2.6e-48
Identity = 119/266 (44.74%), Postives = 161/266 (60.53%), Query Frame = 0


Query: 7   YFLLALYVGGCVVEADVSFSMLGATSETYKQFIKNLRSALTVGSPTVYNIPVLKSTATGP 66
           +F LA+ +G      DVSF +  AT ++Y  FI  LR AL     TV  IP+L STA+G 
Sbjct: 5   FFFLAISLGSPTAIGDVSFDLSTATKKSYSSFITQLRDALPT-QGTVCGIPLLPSTASGS 64

Query: 67  AGFLLVHLTNYNDESITVAIDKPTTFYL--KASNIQDFL---TTEANNLLFKGLKHTRLP 126
             F   +LTNYNDE++TVA++    + +  +A  +  F      EA  L+F G K  +LP
Sbjct: 65  QWFRFFNLTNYNDETVTVAVNVTNVYIVAYRADAVSYFFEDTPAEAFKLIFAGTKTVKLP 124

Query: 127 YKGNYDGLETAAGKISREKIELGFSEISSAIGNMFHHNAGTSVPKAFIVIIETVSEAARY 186
           Y GNYD L++  GK  R+ IELG   +SSAI NM +++   S   A +V+I+  +EAARY
Sbjct: 125 YSGNYDKLQSVVGK-QRDMIELGIPALSSAITNMVYYDY-QSTAAALLVLIQCTAEAARY 184

Query: 187 KYIEQRVSENVKTKFLPDPAFLSLENRWSDLSQQVQIAQTRG-GQFARAVELRTVGNKPV 246
           KYIEQ+VS ++ + F P+ A +SLEN+W  LS+Q+QIA   G GQF   VEL        
Sbjct: 185 KYIEQQVSSHISSNFYPNQAVISLENKWGALSKQIQIANRTGHGQFENPVELYNPDGTRF 244

Query: 247 LVTNVSSPIVKG-IALLLYYKPSVAA 266
            VTN S+ +VKG I LLLYYK SV +
Sbjct: 245 SVTNTSAGVVKGNIKLLLYYKASVGS 267

[Close]

BLAST of Cla97C08G147405 vs. ExPASy Swiss-Prot
Match: P09989 (Ribosome-inactivating protein alpha-trichosanthin OS=Trichosanthes kirilowii OX=3677 PE=1 SV=2)

HSP 1 Score: 193.0 bits (489), Expect = 4.4e-48
Identity = 117/264 (44.32%), Postives = 162/264 (61.36%), Query Frame = 0


Query: 9   LLALYVGGCVVEADVSFSMLGATSETYKQFIKNLRSALTVGSPTVYNIPVLKSTATGPAG 68
           +L L++    VE DVSF + GATS +Y  FI NLR AL      +Y+IP+L+S+  G   
Sbjct: 11  ILTLFLTTPAVEGDVSFRLSGATSSSYGVFISNLRKALP-NERKLYDIPLLRSSLPGSQR 70

Query: 69  FLLVHLTNYNDESITVAIDKPTTFYL--KASNIQDFL----TTEANNLLFK-GLKHTRLP 128
           + L+HLTNY DE+I+VAID    + +  +A +   F      TEA   +FK  ++   LP
Sbjct: 71  YALIHLTNYADETISVAIDVTNVYIMGYRAGDTSYFFNEASATEAAKYVFKDAMRKVTLP 130

Query: 129 YKGNYDGLETAAGKISREKIELGFSEISSAIGNMFHHNAGTSVPKAFIVIIETVSEAARY 188
           Y GNY+ L+TAAGKI RE I LG   + SAI  +F++NA  S   A +V+I++ SEAARY
Sbjct: 131 YSGNYERLQTAAGKI-RENIPLGLPALDSAITTLFYYNA-NSAASALMVLIQSTSEAARY 190

Query: 189 KYIEQRVSENVKTKFLPDPAFLSLENRWSDLSQQVQIAQTRGGQFARAVELRTVGNKPVL 248
           K+IEQ++ + V   FLP  A +SLEN WS LS+Q+QIA T  GQF   V L    N+ V 
Sbjct: 191 KFIEQQIGKRVDKTFLPSLAIISLENSWSALSKQIQIASTNNGQFESPVVLINAQNQRVT 250

Query: 249 VTNVSSPIVKGIALLLYYKPSVAA 266
           +TNV + +V     LL  + ++AA
Sbjct: 251 ITNVDAGVVTSNIALLLNRNNMAA 271

[Close]

BLAST of Cla97C08G147405 vs. ExPASy Swiss-Prot
Match: P24817 (Ribosome-inactivating protein beta-momorcharin OS=Momordica charantia OX=3673 GN=MAP30 PE=1 SV=2)

HSP 1 Score: 192.6 bits (488), Expect = 5.7e-48
Identity = 119/269 (44.24%), Postives = 174/269 (64.68%), Query Frame = 0


Query: 3   LLGFYFLLALYVGGCVVEADVSFSMLGATSETYKQFIKNLRSALTVGSPTVYNIPVLKST 62
           LL  + ++A+++G    + DV+F +  AT++TY +FI++ R+ L   S  VY+IP+L ST
Sbjct: 5   LLLSFLIIAIFIGVPTAKGDVNFDLSTATAKTYTKFIEDFRATLPF-SHKVYDIPLLYST 64

Query: 63  ATGPAGFLLVHLTNYNDESITVAIDKPTTFYLKASNIQDF------LTTEANNLLFKGLK 122
            +    F+L++LT+Y  E+I+VAID  T  Y+ A   +D          EA N+LFKG +
Sbjct: 65  ISDSRRFILLNLTSYAYETISVAID-VTNVYVVAYRTRDVSYFFKESPPEAYNILFKGTR 124

Query: 123 HTRLPYKGNYDGLETAAGKISREKIELGFSEISSAIGNMFHHNAGTSVPKAFIVIIETVS 182
              LPY GNY+ L+TAA KI RE I+LG   +SSAI  +F++NA  S P A +V+I+T +
Sbjct: 125 KITLPYTGNYENLQTAAHKI-RENIDLGLPALSSAITTLFYYNA-QSAPSALLVLIQTTA 184

Query: 183 EAARYKYIEQRVSENVKTKFLPDPAFLSLENRWSDLSQQVQIAQTRGGQFARAVELRTVG 242
           EAAR+KYIE+ V++ V T F P+ A +SLEN+WS LS+Q+ +AQ +GG+F   V+L    
Sbjct: 185 EAARFKYIERHVAKYVATNFKPNLAIISLENQWSALSKQIFLAQNQGGKFRNPVDLIKPT 244

Query: 243 NKPVLVTNVSSPIVKG-IALLLYYKPSVA 265
            +   VTNV S +VKG I LLL  + S A
Sbjct: 245 GERFQVTNVDSDVVKGNIKLLLNSRASTA 269

[Close]

BLAST of Cla97C08G147405 vs. ExPASy TrEMBL
Match: A0A6J1DWK4 (rRNA N-glycosidase OS=Momordica charantia OX=3673 GN=LOC111024728 PE=3 SV=1)

HSP 1 Score: 389.4 bits (999), Expect = 1.2e-104
Identity = 203/269 (75.46%), Postives = 228/269 (84.76%), Query Frame = 0


Query: 1   MRLLGFYFLLALYVGGCVVEADVSFSMLGATSETYKQFIKNLRSALTVGSPTVYNIPVLK 60
           MR L FYF+LAL  G    EADVSFSMLGATSETY+QFI+NLRS LT+ SP VY IPVL+
Sbjct: 1   MRFLPFYFVLALCFGALAAEADVSFSMLGATSETYRQFIRNLRSTLTIRSPVVYGIPVLQ 60

Query: 61  STATGPAGFLLVHLTNYNDESITVAIDKPTTFYL--KASNIQDFL---TTEANNLLFKGL 120
           STA G A F+LVHLTNYN ESITVAID    + +  +A N   FL   + EANN+LFKG+
Sbjct: 61  STAVGSARFILVHLTNYNTESITVAIDVVNIYVVAYRAGNNAYFLNDASAEANNVLFKGI 120

Query: 121 KHTRLPYKGNYDGLETAAGKISREKIELGFSEISSAIGNMFHHNAGTSVPKAFIVIIETV 180
           KH RLPYKGNYDGLETAAGKISREKI+LGFSEISS+IGNMFHHN GTS+ +AFIVII++V
Sbjct: 121 KHVRLPYKGNYDGLETAAGKISREKIDLGFSEISSSIGNMFHHNVGTSIARAFIVIIQSV 180

Query: 181 SEAARYKYIEQRVSENVKTKFLPDPAFLSLENRWSDLSQQVQIAQTRGGQFARAVELRTV 240
            EAAR+KYIEQRVSENVKTKF PDPAFLSLENRWSDLS+Q+QIAQ R G+FARA+ELRTV
Sbjct: 181 LEAARFKYIEQRVSENVKTKFKPDPAFLSLENRWSDLSEQIQIAQKREGEFARAIELRTV 240

Query: 241 GNKPVLVTNVSSPIVKGIALLLYYKPSVA 265
            NKP++VTNVSSPIVKGIALLLYYK  VA
Sbjct: 241 SNKPIIVTNVSSPIVKGIALLLYYKVRVA 269

[Close]

BLAST of Cla97C08G147405 vs. ExPASy TrEMBL
Match: A0A6J1DVE7 (rRNA N-glycosidase OS=Momordica charantia OX=3673 GN=LOC111024729 PE=3 SV=1)

HSP 1 Score: 384.8 bits (987), Expect = 2.9e-103
Identity = 204/268 (76.12%), Postives = 231/268 (86.19%), Query Frame = 0


Query: 1   MRLLGFYFLLALYVGGCVVEADVSFSMLGATSETYKQFIKNLRSALTVGSPTVYNIPVLK 60
           MRLL FYF LALY+G    EADVSFSMLG+TSETYKQFIKN+RSALTVGS  VY+IPVLK
Sbjct: 1   MRLLPFYFFLALYLGARAAEADVSFSMLGSTSETYKQFIKNVRSALTVGSQIVYDIPVLK 60

Query: 61  STATGPAGFLLVHLTNYNDESITVAIDKPTTFYL--KASNIQDFL---TTEANNLLFKGL 120
           +TATG A FLLVHLTNYN ESI VAID    + +  +A N   FL   + EA+ +LF+G+
Sbjct: 61  ATATGSARFLLVHLTNYNSESIIVAIDVVNVYVVAYRAGNNAYFLNDASAEADRVLFQGI 120

Query: 121 KHTRLPYKGNYDGLETAAGKISREKIELGFSEISSAIGNMFHHNAGTSVPKAFIVIIETV 180
            H +L YKGNYDGLETAA KISREKI+LGFSEISS+IGNMFHHNAGTSVP+AFIVII++V
Sbjct: 121 NHVKLSYKGNYDGLETAA-KISREKIDLGFSEISSSIGNMFHHNAGTSVPRAFIVIIQSV 180

Query: 181 SEAARYKYIEQRVSENVKTKFLPDPAFLSLENRWSDLSQQVQIAQTRGGQFARAVELRTV 240
           SEAAR+KYIEQRVSENVKT F PDPAFLSL+NRWSDLS+Q+QIAQ RGG+FARAVELRTV
Sbjct: 181 SEAARFKYIEQRVSENVKTTFKPDPAFLSLQNRWSDLSEQIQIAQKRGGEFARAVELRTV 240

Query: 241 GNKPVLVTNVSSPIVKGIALLLYYKPSV 264
            NKP+LVT+VSSP+VKGIALLLYYK SV
Sbjct: 241 SNKPILVTDVSSPVVKGIALLLYYKVSV 267

[Close]

BLAST of Cla97C08G147405 vs. ExPASy TrEMBL
Match: B1B626 (rRNA N-glycosidase OS=Trichosanthes kirilowii var. japonica OX=61011 PE=2 SV=1)

HSP 1 Score: 383.3 bits (983), Expect = 8.5e-103
Identity = 199/269 (73.98%), Postives = 229/269 (85.13%), Query Frame = 0


Query: 1   MRLLGFYFLLALYVGGCVVEADVSFSMLGATSETYKQFIKNLRSALTVGSPTVYNIPVLK 60
           M+L+GFY +LALYVG  V EADV+FS+LG+TS TYKQFI+NLR+ALTVGSPTV NIPVLK
Sbjct: 1   MKLVGFYLVLALYVGAQVTEADVNFSLLGSTSATYKQFIQNLRAALTVGSPTVENIPVLK 60

Query: 61  STATGPAGFLLVHLTNYNDESITVAIDKPTTFYL--KASNIQDFL---TTEANNLLFKGL 120
            TA G A FLLVHLTNYN ESITVA+D    + +  +A N   FL   + EANN+LFKG+
Sbjct: 61  GTAAGLARFLLVHLTNYNGESITVAVDVVNVYVVAYRAGNNAYFLNDASAEANNVLFKGI 120

Query: 121 KHTRLPYKGNYDGLETAAGKISREKIELGFSEISSAIGNMFHHNAGTSVPKAFIVIIETV 180
            H RLPY GNYDGLETAAG+ISREKI LGFSEISSAIG+MFH+NAGTSVPKAFIVII+TV
Sbjct: 121 NHVRLPYGGNYDGLETAAGRISREKIYLGFSEISSAIGSMFHYNAGTSVPKAFIVIIQTV 180

Query: 181 SEAARYKYIEQRVSENVKTKFLPDPAFLSLENRWSDLSQQVQIAQTRGGQFARAVELRTV 240
           SEAAR+KYI+QRVSENV+TKFLPDPAFLSLEN W  LS+Q+QIAQ RGG+FAR +ELRTV
Sbjct: 181 SEAARFKYIQQRVSENVQTKFLPDPAFLSLENAWGGLSEQIQIAQYRGGEFARPIELRTV 240

Query: 241 GNKPVLVTNVSSPIVKGIALLLYYKPSVA 265
            N P  VTNV+SP+VKG+ALLLYYK +VA
Sbjct: 241 SNTPTFVTNVNSPVVKGVALLLYYKVNVA 269

[Close]

BLAST of Cla97C08G147405 vs. ExPASy TrEMBL
Match: A0A6J1E093 (rRNA N-glycosidase OS=Momordica charantia OX=3673 GN=LOC111024726 PE=3 SV=1)

HSP 1 Score: 326.2 bits (835), Expect = 1.2e-85
Identity = 166/246 (67.48%), Postives = 206/246 (83.74%), Query Frame = 0


Query: 21  ADVSFSMLGATSETYKQFIKNLRSALTVGSPTVYNIPVLKSTATGPAGFLLVHLTNYNDE 80
           ++VSFS+LGAT +TY+QFI+NLR+ALT+ S  VYNIPVL +TA   A F+LVHLTNY +E
Sbjct: 35  SNVSFSLLGATGKTYQQFIQNLRNALTINSKIVYNIPVLAATALSSARFILVHLTNYKNE 94

Query: 81  SITVAIDKPTTFYL--KASNIQDFL---TTEANNLLFKGLKHTRLPYKGNYDGLETAAGK 140
           +ITVAID    + +  +A N   FL   + EA ++LFKG+K   LPYKGNYDGLETAAGK
Sbjct: 95  TITVAIDVVNVYIVAYQAGNKAYFLKDASKEARDVLFKGIKQEILPYKGNYDGLETAAGK 154

Query: 141 ISREKIELGFSEISSAIGNMFHHNAGTSVPKAFIVIIETVSEAARYKYIEQRVSENVKTK 200
           ISREKI+LGFSE+ SAIGNM+H+NAGTSVP+AFIV+I+T+SEAAR+KYIE +VS+NV+TK
Sbjct: 155 ISREKIDLGFSELGSAIGNMYHYNAGTSVPRAFIVMIQTISEAARFKYIETKVSQNVETK 214

Query: 201 FLPDPAFLSLENRWSDLSQQVQIAQTRGGQFARAVELRTVGNKPVLVTNVSSPIVKGIAL 260
           F PDP FLSLENRWSDLS+QVQIAQ R G+FAR +E+R+V NKP+LVTNV S +V+G+AL
Sbjct: 215 FKPDPGFLSLENRWSDLSEQVQIAQNRKGEFARPIEVRSVTNKPILVTNVKSKVVEGMAL 274

Query: 261 LLYYKP 262
           LLY KP
Sbjct: 275 LLYSKP 280

[Close]

BLAST of Cla97C08G147405 vs. ExPASy TrEMBL
Match: A0A6J1IUB2 (rRNA N-glycosidase OS=Cucurbita maxima OX=3661 GN=LOC111480641 PE=3 SV=1)

HSP 1 Score: 295.0 bits (754), Expect = 3.0e-76
Identity = 156/248 (62.90%), Postives = 197/248 (79.44%), Query Frame = 0


Query: 21  ADVSFSMLGATSETYKQFIKNLRSALTVGSPTVYNIPVLKSTATGPAGFLLVHLTNYNDE 80
           A++SFS+LGAT++TYKQFI +LR ALTV S  VY+IPVL +TA G A F+LV+LTNY  E
Sbjct: 34  ANLSFSLLGATAQTYKQFISDLRKALTVNSHKVYDIPVLPATAKGLARFILVNLTNYKGE 93

Query: 81  SITVAIDKPTTFYL--KASNIQDFL---TTEANNLLFKGLKHTRLPYKGNYDGLETAAGK 140
           SITVAID    + +  +A N   FL   + EA+++LFKG KH  L YKGNYD LE  AGK
Sbjct: 94  SITVAIDTVDVYIVAYQAGNKAYFLKDVSKEAHDVLFKGSKHETLSYKGNYDDLERHAGK 153

Query: 141 ISREKIELGFSEISSAIGNMFHHNAGTSVPKAFIVIIETVSEAARYKYIEQRVSENVKTK 200
           ISRE I+LGFSE+SS+IGNMFH+NAG SVPK+FIV+I+ +SEA+R+KYIE R  ENV++K
Sbjct: 154 ISRENIDLGFSELSSSIGNMFHYNAGYSVPKSFIVMIQMLSEASRFKYIEARNCENVESK 213

Query: 201 FLPDPAFLSLENRWSDLSQQVQIAQTRGGQFARAVELRTVGNKPVLVTNVSSPIVKGIAL 260
           F PDP +LSLENRWS+LS+QVQ AQ  GG+F   V++R+V NKPVLV++V+SP+VKG+AL
Sbjct: 214 FKPDPGYLSLENRWSNLSEQVQKAQKHGGKFEHPVQVRSVSNKPVLVSDVNSPVVKGMAL 273

Query: 261 LLYYKPSV 264
           LL  KP V
Sbjct: 274 LLCNKPGV 281

The following BLAST results are available for this feature:

BLAST of Cla97C08G147405 vs. NCBI nr


Match Name	E-value	Identity	Description
XP_022158192.1	2.4e-104	75.46	ribosome-inactivating protein bryodin II-like [Momordica charantia]	[more]
XP_022158193.1	6.0e-103	76.12	ribosome-inactivating protein bryodin II-like [Momordica charantia]	[more]
BAG12408.1	1.7e-102	73.98	karasurin-H precursor [Trichosanthes kirilowii var. japonica]	[more]
P98184.1	1.1e-93	68.77	RecName: Full=Ribosome-inactivating protein bryodin II; AltName: Full=BD2; AltNa...	[more]
XP_022158191.1	2.5e-85	67.48	ribosome-inactivating protein bryodin II-like [Momordica charantia]	[more]

BLAST of Cla97C08G147405 vs. ExPASy Swiss-Prot


Match Name	E-value	Identity	Description
P98184	1.5e-96	68.77	Ribosome-inactivating protein bryodin II OS=Bryonia dioica OX=3652 PE=1 SV=1	[more]
Q00465	4.0e-49	45.08	Ribosome-inactivating protein luffin-alpha OS=Luffa aegyptiaca OX=3670 PE=1 SV=1	[more]
P56626	2.6e-48	44.74	Type I ribosome-inactivating protein trichoanguina OS=Trichosanthes anguina OX=5...	[more]
P09989	4.4e-48	44.32	Ribosome-inactivating protein alpha-trichosanthin OS=Trichosanthes kirilowii OX=...	[more]
P24817	5.7e-48	44.24	Ribosome-inactivating protein beta-momorcharin OS=Momordica charantia OX=3673 GN...	[more]

BLAST of Cla97C08G147405 vs. ExPASy TrEMBL


Match Name	E-value	Identity	Description
A0A6J1DWK4	1.2e-104	75.46	rRNA N-glycosidase OS=Momordica charantia OX=3673 GN=LOC111024728 PE=3 SV=1	[more]
A0A6J1DVE7	2.9e-103	76.12	rRNA N-glycosidase OS=Momordica charantia OX=3673 GN=LOC111024729 PE=3 SV=1	[more]
B1B626	8.5e-103	73.98	rRNA N-glycosidase OS=Trichosanthes kirilowii var. japonica OX=61011 PE=2 SV=1	[more]
A0A6J1E093	1.2e-85	67.48	rRNA N-glycosidase OS=Momordica charantia OX=3673 GN=LOC111024726 PE=3 SV=1	[more]
A0A6J1IUB2	3.0e-76	62.90	rRNA N-glycosidase OS=Cucurbita maxima OX=3661 GN=LOC111480641 PE=3 SV=1	[more]

BLAST of Cla97C08G147405 vs. ExPASy TrEMBL


Match Name	E-value	Identity	Description

InterPro

Analysis Name: InterPro Annotations of Watermelon (97103) v2.5
Date Performed: 2022-01-31


IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
IPR017989	Ribosome-inactivating protein type 1/2	PRINTS	PR00396	SHIGARICIN	coord: 81..96 score: 22.37 coord: 200..217 score: 44.3 coord: 168..188 score: 54.51 coord: 138..152 score: 44.04 coord: 33..46 score: 41.73
IPR016138	Ribosome-inactivating protein, subdomain 1	GENE3D	3.40.420.10	Ricin (A subunit), domain 1	coord: 22..180 e-value: 9.2E-31 score: 108.8
IPR016139	Ribosome-inactivating protein, subdomain 2	GENE3D	4.10.470.10	Ricin (A Subunit), domain 2	coord: 181..264 e-value: 3.7E-27 score: 96.0
IPR001574	Ribosome-inactivating protein	PFAM	PF00161	RIP	coord: 31..217 e-value: 5.4E-24 score: 84.9
IPR001574	Ribosome-inactivating protein	PANTHER	PTHR33453	FAMILY NOT NAMED	coord: 23..256
None	No IPR available	PANTHER	PTHR33453:SF14	RRNA N-GLYCOSIDASE	coord: 23..256
IPR017988	Ribosome-inactivating protein conserved site	PROSITE	PS00275	SHIGA_RICIN	coord: 172..188
IPR036041	Ribosome-inactivating protein superfamily	SUPERFAMILY	56371	Ribosome inactivating proteins (RIP)	coord: 22..262

Relationships

The following mRNA feature(s) are a part of this gene:


Feature Name	Unique Name	Type
Cla97C08G147405.1	Cla97C08G147405.1	mRNA

GO Annotation

GO Assignments

This gene is annotated with the following GO terms.

Category	Term Accession	Term Name
biological_process	GO:0006952	defense response
biological_process	GO:0035821	modulation of process of other organism
biological_process	GO:0017148	negative regulation of translation
molecular_function	GO:0030598	rRNA N-glycosylase activity
molecular_function	GO:0090729	toxin activity