Homology
BLAST of Cla97C08G145420 vs. NCBI nr
Match:
XP_038890278.1 (non-lysosomal glucosylceramidase-like isoform X1 [Benincasa hispida])
HSP 1 Score: 1686.4 bits (4366), Expect = 0.0e+00
Identity = 833/1001 (83.22%), Postives = 861/1001 (86.01%), Query Frame = 0
Query: 1 MEKGGNGASSTEVDPSKPPSLTWKRKLNFTGKSPESFSFTLTDAWHLGSLGYRLWRHGKE 60
MEKGGNGASST VDPSKPPSLTWKRKL+FTGKSPESFSFTLTDAWHLGSLGYRLWRHGKE
Sbjct: 1 MEKGGNGASSTTVDPSKPPSLTWKRKLDFTGKSPESFSFTLTDAWHLGSLGYRLWRHGKE 60
Query: 61 EAAKGRVIMTASANFFQIDSVFLFHLIIVSLYSIFSLSLSLSLCPQIPLYEFFSDQPITC 120
E AKGR IP+YEFFSDQPITC
Sbjct: 61 ETAKGR----------------------------------------IPVYEFFSDQPITC 120
Query: 121 YHGVSLGGIGAGSIGRSYRGEFQRFQMFYGPCEDEPILANQFSVFVSRPNGKKFSSVLCS 180
YHGVSLGGIGAGSIGRSYRGEFQRFQMFYGPCEDEP+LANQFSVFVSRPNG KFSSVLC
Sbjct: 121 YHGVSLGGIGAGSIGRSYRGEFQRFQMFYGPCEDEPVLANQFSVFVSRPNGNKFSSVLCF 180
Query: 181 TKPQKSKDGKQDGIESWDWNLSGENSTYHALFPRSWTVYDGEPDPDLKIVCRQISPFIPH 240
KPQKSKDGKQDGIESWDWNLSG NSTYHALFPRSWT+YDGEPDPDLKIVCRQISPFIPH
Sbjct: 181 AKPQKSKDGKQDGIESWDWNLSGANSTYHALFPRSWTIYDGEPDPDLKIVCRQISPFIPH 240
Query: 241 NYKESSFPVSVFTFSLFNEGQTSAQVTLLFTWAVSAANYFLFNFLYFIFITVNSVGGKSG 300
NYKESSFPVSVFTF+L NEGQTSAQVTLLFTWA NSVGGKSG
Sbjct: 241 NYKESSFPVSVFTFNLSNEGQTSAQVTLLFTWA-------------------NSVGGKSG 300
Query: 301 FTGHHFNSKIGAEDGAQGVLLHHKTANGRPSVTYAIAAEATDDVHVSLCPCFVISGESEG 360
FTGHHFNSK+GAEDGA GVLLHHKT GRP+VTYAIAAE TDDVH+S+CPCFVISG+SEG
Sbjct: 301 FTGHHFNSKMGAEDGAHGVLLHHKTTKGRPTVTYAIAAEETDDVHISVCPCFVISGDSEG 360
Query: 361 ISAKDMWQEIKNHGSFDKLGSVGAYEGSKPGSSIGAAIAATLTVPPRSARTVTFSLAWDC 420
ISAKDMWQEIKNHGSFDKLGSVG YEGSKPGSSIGAA+AATLT+P ARTVTFSLAWDC
Sbjct: 361 ISAKDMWQEIKNHGSFDKLGSVGQYEGSKPGSSIGAAVAATLTIPSSVARTVTFSLAWDC 420
Query: 421 PEVKFDGKTYHR-----------------------------------------------Y 480
PEVKFDGKTYHR Y
Sbjct: 421 PEVKFDGKTYHRRYSKFYGTLGDAAKIIARDAILEHGKWEAQIEAWQRPIIEDKRLPEWY 480
Query: 481 PVTLFNELYFLNSGGTIWTDGLAPLQNLSTISDKKYFLERSKSHPNGGAPTADHRKDVAV 540
PVTL NELYFLN+GGTIWTDGL PLQNLSTI+ +KYFLERSKS PNGGA DHRKDVAV
Sbjct: 481 PVTLLNELYFLNAGGTIWTDGLPPLQNLSTITHQKYFLERSKSEPNGGALNGDHRKDVAV 540
Query: 541 DILERMSQIFDQTHGGAGPSNAALGTRLLHPGEENVGNLLLVEGSQYLMWNTYDVHFYSS 600
DILERMSQIFDQTHGGAGPSNAALGTRLLHPGEENVGNLLLVEGSQYLMWNTYDVHFYSS
Sbjct: 541 DILERMSQIFDQTHGGAGPSNAALGTRLLHPGEENVGNLLLVEGSQYLMWNTYDVHFYSS 600
Query: 601 FALIMLLPKLELSIQRDFAAAVLMHDPRKTKIMSDGNWVPRKVLGAVPHDIGFNDPWFEV 660
FAL+ML PKLELSIQRDFAAAVLMHDPRK KIMSDGNWVPRKVLGAVPHD+GFNDPWFEV
Sbjct: 601 FALVMLFPKLELSIQRDFAAAVLMHDPRKAKIMSDGNWVPRKVLGAVPHDVGFNDPWFEV 660
Query: 661 NAYNLLNVTRWKDLASKFVLQVYRDVVATGDKNFAQSVWPSVYVALAFMEQFDKDKDGMV 720
NAYNLLNVTRWKDL SKFVLQVYRDVVATGDKNFAQSVWPSVYVALAFMEQFDKDKDGM+
Sbjct: 661 NAYNLLNVTRWKDLGSKFVLQVYRDVVATGDKNFAQSVWPSVYVALAFMEQFDKDKDGMI 720
Query: 721 ENEGFPDQTYDTWTVKGVSAYCGGLWVAALQAASALASEVDDDAAAHYFWIKYQKARSVY 780
ENEGFPDQTYDTWTVKGVSAYCGGLWVAALQAASALASEVDD+AAAHYFW KYQKA+SVY
Sbjct: 721 ENEGFPDQTYDTWTVKGVSAYCGGLWVAALQAASALASEVDDEAAAHYFWTKYQKAKSVY 780
Query: 781 DTLWNGSYFNYDNSKGPWSSSIQADQLAGQWYARACGLCPIADEEKIRSALEKIYNFN-- 840
DTLWNGSYFNYDNSKGPWSSSIQADQLAGQWYARACGLCP+ADEEKIRSALEKIYNFN
Sbjct: 781 DTLWNGSYFNYDNSKGPWSSSIQADQLAGQWYARACGLCPVADEEKIRSALEKIYNFNVM 840
Query: 841 -VKGGTRGAVNGMFPDGRVDKSILQPKEIWAGVTYSVAASMIQEGLVETGFQTAMGIHQA 900
VKGGTRGAVNGMFPDGRVDKSILQPKEIW GVTYSVAASMIQEGLVETGFQTAMGIHQA
Sbjct: 841 KVKGGTRGAVNGMFPDGRVDKSILQPKEIWPGVTYSVAASMIQEGLVETGFQTAMGIHQA 900
Query: 901 AWAQDGLGYSFQTPEAWDVDDRYRSIGYMRPMAIWAMQWALMSKKPTKIPTKALSKMEET 952
AWAQDGLGYSFQTPEAWDVDDRYRSIGYMRP+AIWAMQWA+ KPTKIPTK LS+MEE+
Sbjct: 901 AWAQDGLGYSFQTPEAWDVDDRYRSIGYMRPLAIWAMQWAI--SKPTKIPTKVLSEMEES 940
BLAST of Cla97C08G145420 vs. NCBI nr
Match:
XP_004141546.1 (non-lysosomal glucosylceramidase isoform X1 [Cucumis sativus] >KGN52666.1 hypothetical protein Csa_009021 [Cucumis sativus])
HSP 1 Score: 1664.8 bits (4310), Expect = 0.0e+00
Identity = 828/1003 (82.55%), Postives = 857/1003 (85.44%), Query Frame = 0
Query: 1 MEKGGNGASSTEVDPSKPPSLTWKRKLNFTGKSPESFSFTLTDAWHLGSLGYRLWRHGKE 60
MEKGGNGASSTEVDPSKPPSLTWKRKL+FTGKSPESFSFTLTDAWH+G GYRLWR+GKE
Sbjct: 1 MEKGGNGASSTEVDPSKPPSLTWKRKLDFTGKSPESFSFTLTDAWHMGMTGYRLWRNGKE 60
Query: 61 EAAKGRVIMTASANFFQIDSVFLFHLIIVSLYSIFSLSLSLSLCPQIPLYEFFSDQPITC 120
E AKGR IP+YEFFSD PITC
Sbjct: 61 EIAKGR----------------------------------------IPIYEFFSDVPITC 120
Query: 121 YHGVSLGGIGAGSIGRSYRGEFQRFQMFYGPCEDEPILANQFSVFVSRPNGKKFSSVLCS 180
YHGVSLGGIGAGSIGRSYRGEFQRFQMFYGPCEDEP+LANQFSVFVSRPNG KFSSVLCS
Sbjct: 121 YHGVSLGGIGAGSIGRSYRGEFQRFQMFYGPCEDEPVLANQFSVFVSRPNGNKFSSVLCS 180
Query: 181 TKPQKSKDGKQDGIESWDWNLSGENSTYHALFPRSWTVYDGEPDPDLKIVCRQISPFIPH 240
KPQKSKDGKQ GIESWDWNLSGENSTYHALFPRSWTVYDGEPDPDLKIVCRQ+SP IPH
Sbjct: 181 AKPQKSKDGKQTGIESWDWNLSGENSTYHALFPRSWTVYDGEPDPDLKIVCRQMSPIIPH 240
Query: 241 NYKESSFPVSVFTFSLFNEGQTSAQVTLLFTWAVSAANYFLFNFLYFIFITVNSVGGKSG 300
NYKESSFPVSVFTF L NEGQTSAQVTLLFTWA NSVGGKSG
Sbjct: 241 NYKESSFPVSVFTFKLSNEGQTSAQVTLLFTWA-------------------NSVGGKSG 300
Query: 301 FTGHHFNSKIGAEDGAQGVLLHHKTANGRPSVTYAIAAEATDDVHVSLCPCFVISGESEG 360
FTGHHFNSK+GAEDGAQGVLLHHK+ANGRP+VTY IAAEATDDVHVSLCPCFVISG+SEG
Sbjct: 301 FTGHHFNSKMGAEDGAQGVLLHHKSANGRPTVTYGIAAEATDDVHVSLCPCFVISGDSEG 360
Query: 361 ISAKDMWQEIKNHGSFDKLGSVGAYEGSKPGSSIGAAIAATLTVPPRSARTVTFSLAWDC 420
ISAKDMWQEIKNHGSFD LGSVGA EGSKPG SIGAA+AATLT+PP SARTVTFSLAWDC
Sbjct: 361 ISAKDMWQEIKNHGSFDNLGSVGANEGSKPGCSIGAAVAATLTIPPTSARTVTFSLAWDC 420
Query: 421 PEVKFDGKTYHR-----------------------------------------------Y 480
PEVKFDGKTYHR Y
Sbjct: 421 PEVKFDGKTYHRQYSKFYGTLGDAAEIIARDAISKHGKWEAEIEAWQRPIIEDKRLPDWY 480
Query: 481 PVTLFNELYFLNSGGTIWTDGLAPLQNLSTISDKKYFLERSKSHPNGGAPTADHRKDVAV 540
PVTLFNELYFLNSGGTIWTDGL PLQNLSTIS KKYFLERSKS NGGAP DHRKDVAV
Sbjct: 481 PVTLFNELYFLNSGGTIWTDGLPPLQNLSTISHKKYFLERSKSELNGGAPNGDHRKDVAV 540
Query: 541 DILERMSQIFDQTHGGAGPSNAALGTRLLHPGEENVGNLLLVEGSQYLMWNTYDVHFYSS 600
DILERMSQI DQTHGGAGPSNAALGTRLLHPGEENVGNLLLVEGSQYLMWNTYDVHFYSS
Sbjct: 541 DILERMSQILDQTHGGAGPSNAALGTRLLHPGEENVGNLLLVEGSQYLMWNTYDVHFYSS 600
Query: 601 FALIMLLPKLELSIQRDFAAAVLMHDPRKTKIMSDGNWVPRKVLGAVPHDIGFNDPWFEV 660
FALIML PKLELSIQRDFAAAVLMHDPRK K M DGNWVPRKVLGAVPHDIGFNDPW EV
Sbjct: 601 FALIMLFPKLELSIQRDFAAAVLMHDPRKAKTMCDGNWVPRKVLGAVPHDIGFNDPWLEV 660
Query: 661 NAYNLLNVTRWKDLASKFVLQVYRDVVATGDKNFAQSVWPSVYVALAFMEQFDKDKDGMV 720
NAYNLLNV+RWKDL SKFVLQVYRDVVATGDKNFA+SVWPSVYVALAFMEQFDKDKDGM+
Sbjct: 661 NAYNLLNVSRWKDLGSKFVLQVYRDVVATGDKNFAKSVWPSVYVALAFMEQFDKDKDGMI 720
Query: 721 ENEGFPDQTYDTWTVKGVSAYCGGLWVAALQAASALASEVDDDAAAHYFWIKYQKARSVY 780
ENEGFPDQTYDTWTVKGVSAYCGGLWVAALQAASALASEVDD+AAAHYFWIKYQKARSVY
Sbjct: 721 ENEGFPDQTYDTWTVKGVSAYCGGLWVAALQAASALASEVDDEAAAHYFWIKYQKARSVY 780
Query: 781 DTLWNGSYFNYDNSKGPWSSSIQADQLAGQWYARACGLCPIADEEKIRSALEKIYNFN-- 840
+TLWNGSYFNYDNSKGPWSSSIQADQLAGQWYARACGLCPIADEEKIR ALEKIYNFN
Sbjct: 781 ETLWNGSYFNYDNSKGPWSSSIQADQLAGQWYARACGLCPIADEEKIRVALEKIYNFNVM 840
Query: 841 -VKGGTRGAVNGMFPDGRVDKSILQPKEIWAGVTYSVAASMIQEGLVETGFQTAMGIHQA 900
VKGGTRGAVNGMFPDG VDKSILQPKEIWAGVTYSVAA+MIQEG+VETGFQTAMGI+QA
Sbjct: 841 KVKGGTRGAVNGMFPDGSVDKSILQPKEIWAGVTYSVAATMIQEGMVETGFQTAMGIYQA 900
Query: 901 AWAQDGLGYSFQTPEAWDVDDRYRSIGYMRPMAIWAMQWALMS-KKPTKIPTKALSKMEE 952
AWAQDGLGYSFQTPEAWDVDDR+RSIGYMRP+AIWAMQWA+MS KPTK+PTKA S+M+E
Sbjct: 901 AWAQDGLGYSFQTPEAWDVDDRFRSIGYMRPLAIWAMQWAMMSDSKPTKVPTKAFSEMQE 944
BLAST of Cla97C08G145420 vs. NCBI nr
Match:
KAA0039284.1 (non-lysosomal glucosylceramidase isoform X2 [Cucumis melo var. makuwa])
HSP 1 Score: 1654.8 bits (4284), Expect = 0.0e+00
Identity = 823/994 (82.80%), Postives = 852/994 (85.71%), Query Frame = 0
Query: 1 MEKGGNGASSTEVDPSKPPSLTWKRKLNFTGKSPESFSFTLTDAWHLGSLGYRLWRHGKE 60
MEKGGNGASSTEVDPSKPPSLTWKRKL+FTGKSPE FSFTLTDAWH+G LGYRLWR+GKE
Sbjct: 1 MEKGGNGASSTEVDPSKPPSLTWKRKLDFTGKSPECFSFTLTDAWHMGMLGYRLWRNGKE 60
Query: 61 EAAKGRVIMTASANFFQIDSVFLFHLIIVSLYSIFSLSLSLSLCPQIPLYEFFSDQPITC 120
E AKGR IP+YEFFSDQPITC
Sbjct: 61 EIAKGR----------------------------------------IPIYEFFSDQPITC 120
Query: 121 YHGVSLGGIGAGSIGRSYRGEFQRFQMFYGPCEDEPILANQFSVFVSRPNGKKFSSVLCS 180
YHGVSLGGIGAGSIGRSYRGEFQRFQMFYGPCEDEP+LANQFSVFVSRPNG KFSSVLCS
Sbjct: 121 YHGVSLGGIGAGSIGRSYRGEFQRFQMFYGPCEDEPVLANQFSVFVSRPNGNKFSSVLCS 180
Query: 181 TKPQKSKDGKQDGIESWDWNLSGENSTYHALFPRSWTVYDGEPDPDLKIVCRQISPFIPH 240
KPQK KDG Q GI SWDWNLSGENSTYHALFPRSWT+Y+GEPDPDLKIVCRQISP IPH
Sbjct: 181 AKPQKFKDGNQTGIGSWDWNLSGENSTYHALFPRSWTIYNGEPDPDLKIVCRQISPIIPH 240
Query: 241 NYKESSFPVSVFTFSLFNEGQTSAQVTLLFTWAVSAANYFLFNFLYFIFITVNSVGGKSG 300
NYKESSFPVSVFTF+L NEGQTSAQVTLLFTWA NSVGGKSG
Sbjct: 241 NYKESSFPVSVFTFNLSNEGQTSAQVTLLFTWA-------------------NSVGGKSG 300
Query: 301 FTGHHFNSKIGAEDGAQGVLLHHKTANGRPSVTYAIAAEATDDVHVSLCPCFVISGESEG 360
FTGHHFNSK+ AEDGA GVLLHH+TA GRP+VTYAIAAEATDDVHVSLCPCFVISG+SEG
Sbjct: 301 FTGHHFNSKMRAEDGAHGVLLHHRTAGGRPTVTYAIAAEATDDVHVSLCPCFVISGDSEG 360
Query: 361 ISAKDMWQEIKNHGSFDKLGSVGAYEGSKPGSSIGAAIAATLTVPPRSARTVTFSLAWDC 420
ISAKDMWQEIKNHGSFDKLG+V A GSKPGSSIGAA+AATLT+PP SARTVTFSLAWDC
Sbjct: 361 ISAKDMWQEIKNHGSFDKLGNVAANGGSKPGSSIGAAVAATLTIPPASARTVTFSLAWDC 420
Query: 421 PEVKFDGKTYHR--------------------------------------YPVTLFNELY 480
PEVKFDGKTYHR Y VTLFNELY
Sbjct: 421 PEVKFDGKTYHRSYTKFYGTLGDAARTIAHDAILEAWQRPIIEDKRLPEWYAVTLFNELY 480
Query: 481 FLNSGGTIWTDGLAPLQNLSTISDKKYFLERSKSHPNGGAPTADHRKDVAVDILERMSQI 540
FLNSGGTIWTDGL PLQNLSTIS KKYFLERSKS PNGG P HRKDVAVDILERMSQI
Sbjct: 481 FLNSGGTIWTDGLPPLQNLSTISHKKYFLERSKSEPNGGVPNGVHRKDVAVDILERMSQI 540
Query: 541 FDQTHGGAGPSNAALGTRLLHPGEENVGNLLLVEGSQYLMWNTYDVHFYSSFALIMLLPK 600
FDQT GGAGPSNAALGTRLLHPGEENVGNLLLVEGSQYLMWNTYDVHFYSSFALIML PK
Sbjct: 541 FDQTDGGAGPSNAALGTRLLHPGEENVGNLLLVEGSQYLMWNTYDVHFYSSFALIMLFPK 600
Query: 601 LELSIQRDFAAAVLMHDPRKTKIMSDGNWVPRKVLGAVPHDIGFNDPWFEVNAYNLLNVT 660
LELSIQRDFAAAVLMHDPRK KIMSDGNWVPRKVLGAVPHDIGFNDPWFEVNAYNLLNVT
Sbjct: 601 LELSIQRDFAAAVLMHDPRKAKIMSDGNWVPRKVLGAVPHDIGFNDPWFEVNAYNLLNVT 660
Query: 661 RWKDLASKFVLQVYRDVVATGDKNFAQSVWPSVYVALAFMEQFDKDKDGMVENEGFPDQT 720
RWKDL SKFVLQVYRDVVATGDKNFA+SVWPSVYVALAFMEQFDKDKDGM+ENEGFPDQT
Sbjct: 661 RWKDLGSKFVLQVYRDVVATGDKNFAKSVWPSVYVALAFMEQFDKDKDGMIENEGFPDQT 720
Query: 721 YDTWTVKGVSAYCGGLWVAALQAASALASEVDDDAAAHYFWIKYQKARSVYDTLWNGSYF 780
YDTWTVKGVSAYCGGLWVAALQAASALASEVDD+AAAHYFWIKYQKARSVY+TLWNGSYF
Sbjct: 721 YDTWTVKGVSAYCGGLWVAALQAASALASEVDDEAAAHYFWIKYQKARSVYETLWNGSYF 780
Query: 781 NYDNSKGPWSSSIQADQLAGQWYARACGLCPIADEEKIRSALEKIYNFN---VKGGTRGA 840
NYDNSKGPWSSSIQADQLAGQWYARACGLCPIADEEKIR ALEKIYNFN VKGGTRGA
Sbjct: 781 NYDNSKGPWSSSIQADQLAGQWYARACGLCPIADEEKIRGALEKIYNFNVMKVKGGTRGA 840
Query: 841 VNGMFPDGRVDKSILQPKEIWAGVTYSVAASMIQEGLVETGFQTAMGIHQAAWAQDGLGY 900
VNGMFPDG VDKSILQPKEIWAGVTYSVAA+MIQEGLVETGFQTAMG++ AAWAQDGLGY
Sbjct: 841 VNGMFPDGSVDKSILQPKEIWAGVTYSVAATMIQEGLVETGFQTAMGVYHAAWAQDGLGY 900
Query: 901 SFQTPEAWDVDDRYRSIGYMRPMAIWAMQWALMS-KKPTKIPTKALSKMEETAFATQHAA 952
SFQTPEAWDVDDR+RSI YMRP+AIWAMQWA+MS KPTK PTKA S+MEE+AFATQH A
Sbjct: 901 SFQTPEAWDVDDRFRSIAYMRPLAIWAMQWAMMSDSKPTKFPTKAFSEMEESAFATQHTA 935
BLAST of Cla97C08G145420 vs. NCBI nr
Match:
TYK00469.1 (non-lysosomal glucosylceramidase isoform X2 [Cucumis melo var. makuwa])
HSP 1 Score: 1654.8 bits (4284), Expect = 0.0e+00
Identity = 823/994 (82.80%), Postives = 852/994 (85.71%), Query Frame = 0
Query: 1 MEKGGNGASSTEVDPSKPPSLTWKRKLNFTGKSPESFSFTLTDAWHLGSLGYRLWRHGKE 60
MEKGGNGASSTEVDPSKPPSLTWKRKL+FTGKSPE FSFTLTDAWH+G LGYRLWR+GKE
Sbjct: 1 MEKGGNGASSTEVDPSKPPSLTWKRKLDFTGKSPECFSFTLTDAWHMGMLGYRLWRNGKE 60
Query: 61 EAAKGRVIMTASANFFQIDSVFLFHLIIVSLYSIFSLSLSLSLCPQIPLYEFFSDQPITC 120
E AKGR IP+YEFFSDQPITC
Sbjct: 61 EIAKGR----------------------------------------IPIYEFFSDQPITC 120
Query: 121 YHGVSLGGIGAGSIGRSYRGEFQRFQMFYGPCEDEPILANQFSVFVSRPNGKKFSSVLCS 180
YHGVSLGGIGAGSIGRSYRGEFQRFQMFYGPCEDEP+LANQFSVFVSRPNG KFSSVLCS
Sbjct: 121 YHGVSLGGIGAGSIGRSYRGEFQRFQMFYGPCEDEPVLANQFSVFVSRPNGNKFSSVLCS 180
Query: 181 TKPQKSKDGKQDGIESWDWNLSGENSTYHALFPRSWTVYDGEPDPDLKIVCRQISPFIPH 240
KPQK KDG Q GI SWDWNLSGENSTYHALFPRSWT+Y+GEPDPDLKIVCRQISP IPH
Sbjct: 181 AKPQKFKDGNQTGIGSWDWNLSGENSTYHALFPRSWTIYNGEPDPDLKIVCRQISPIIPH 240
Query: 241 NYKESSFPVSVFTFSLFNEGQTSAQVTLLFTWAVSAANYFLFNFLYFIFITVNSVGGKSG 300
NYKESSFPVSVFTF+L NEGQTSAQVTLLFTWA NSVGGKSG
Sbjct: 241 NYKESSFPVSVFTFNLSNEGQTSAQVTLLFTWA-------------------NSVGGKSG 300
Query: 301 FTGHHFNSKIGAEDGAQGVLLHHKTANGRPSVTYAIAAEATDDVHVSLCPCFVISGESEG 360
FTGHHFNSK+ AEDGA GVLLHH+TA GRP+VTYAIAAEATDDVHVSLCPCFVISG+SEG
Sbjct: 301 FTGHHFNSKMRAEDGAHGVLLHHRTAGGRPTVTYAIAAEATDDVHVSLCPCFVISGDSEG 360
Query: 361 ISAKDMWQEIKNHGSFDKLGSVGAYEGSKPGSSIGAAIAATLTVPPRSARTVTFSLAWDC 420
ISAKDMWQEIKNHGSFDKLG+V A GSKPGSSIGAA+AATLT+PP SARTVTFSLAWDC
Sbjct: 361 ISAKDMWQEIKNHGSFDKLGNVAANGGSKPGSSIGAAVAATLTIPPASARTVTFSLAWDC 420
Query: 421 PEVKFDGKTYHR--------------------------------------YPVTLFNELY 480
PEVKFDGKTYHR Y VTLFNELY
Sbjct: 421 PEVKFDGKTYHRQYTKFYGTLGDAARTIAHDAILEAWQRPIIEDKRLPEWYAVTLFNELY 480
Query: 481 FLNSGGTIWTDGLAPLQNLSTISDKKYFLERSKSHPNGGAPTADHRKDVAVDILERMSQI 540
FLNSGGTIWTDGL PLQNLSTIS KKYFLERSKS PNGG P HRKDVAVDILERMSQI
Sbjct: 481 FLNSGGTIWTDGLPPLQNLSTISHKKYFLERSKSEPNGGVPNGVHRKDVAVDILERMSQI 540
Query: 541 FDQTHGGAGPSNAALGTRLLHPGEENVGNLLLVEGSQYLMWNTYDVHFYSSFALIMLLPK 600
FDQT GGAGPSNAALGTRLLHPGEENVGNLLLVEGSQYLMWNTYDVHFYSSFALIML PK
Sbjct: 541 FDQTDGGAGPSNAALGTRLLHPGEENVGNLLLVEGSQYLMWNTYDVHFYSSFALIMLFPK 600
Query: 601 LELSIQRDFAAAVLMHDPRKTKIMSDGNWVPRKVLGAVPHDIGFNDPWFEVNAYNLLNVT 660
LELSIQRDFAAAVLMHDPRK KIMSDGNWVPRKVLGAVPHDIGFNDPWFEVNAYNLLNVT
Sbjct: 601 LELSIQRDFAAAVLMHDPRKAKIMSDGNWVPRKVLGAVPHDIGFNDPWFEVNAYNLLNVT 660
Query: 661 RWKDLASKFVLQVYRDVVATGDKNFAQSVWPSVYVALAFMEQFDKDKDGMVENEGFPDQT 720
RWKDL SKFVLQVYRDVVATGDKNFA+SVWPSVYVALAFMEQFDKDKDGM+ENEGFPDQT
Sbjct: 661 RWKDLGSKFVLQVYRDVVATGDKNFAKSVWPSVYVALAFMEQFDKDKDGMIENEGFPDQT 720
Query: 721 YDTWTVKGVSAYCGGLWVAALQAASALASEVDDDAAAHYFWIKYQKARSVYDTLWNGSYF 780
YDTWTVKGVSAYCGGLWVAALQAASALASEVDD+AAAHYFWIKYQKARSVY+TLWNGSYF
Sbjct: 721 YDTWTVKGVSAYCGGLWVAALQAASALASEVDDEAAAHYFWIKYQKARSVYETLWNGSYF 780
Query: 781 NYDNSKGPWSSSIQADQLAGQWYARACGLCPIADEEKIRSALEKIYNFN---VKGGTRGA 840
NYDNSKGPWSSSIQADQLAGQWYARACGLCPIADEEKIR ALEKIYNFN VKGGTRGA
Sbjct: 781 NYDNSKGPWSSSIQADQLAGQWYARACGLCPIADEEKIRGALEKIYNFNVMKVKGGTRGA 840
Query: 841 VNGMFPDGRVDKSILQPKEIWAGVTYSVAASMIQEGLVETGFQTAMGIHQAAWAQDGLGY 900
VNGMFPDG VDKSILQPKEIWAGVTYSVAA+MIQEGLVETGFQTAMG++ AAWAQDGLGY
Sbjct: 841 VNGMFPDGSVDKSILQPKEIWAGVTYSVAATMIQEGLVETGFQTAMGVYHAAWAQDGLGY 900
Query: 901 SFQTPEAWDVDDRYRSIGYMRPMAIWAMQWALMS-KKPTKIPTKALSKMEETAFATQHAA 952
SFQTPEAWDVDDR+RSI YMRP+AIWAMQWA+MS KPTK PTKA S+MEE+AFATQH A
Sbjct: 901 SFQTPEAWDVDDRFRSIAYMRPLAIWAMQWAMMSDSKPTKFPTKAFSEMEESAFATQHTA 935
BLAST of Cla97C08G145420 vs. NCBI nr
Match:
XP_008459592.1 (PREDICTED: non-lysosomal glucosylceramidase isoform X2 [Cucumis melo])
HSP 1 Score: 1651.3 bits (4275), Expect = 0.0e+00
Identity = 823/1003 (82.05%), Postives = 852/1003 (84.95%), Query Frame = 0
Query: 1 MEKGGNGASSTEVDPSKPPSLTWKRKLNFTGKSPESFSFTLTDAWHLGSLGYRLWRHGKE 60
MEKGGNGASSTEVDPSKPPSLTWKRKL+FTGKSPE FSFTLTDAWH+G LGYRLWR+GKE
Sbjct: 1 MEKGGNGASSTEVDPSKPPSLTWKRKLDFTGKSPECFSFTLTDAWHMGMLGYRLWRNGKE 60
Query: 61 EAAKGRVIMTASANFFQIDSVFLFHLIIVSLYSIFSLSLSLSLCPQIPLYEFFSDQPITC 120
E AKGR IP+YEFFSDQPITC
Sbjct: 61 EIAKGR----------------------------------------IPIYEFFSDQPITC 120
Query: 121 YHGVSLGGIGAGSIGRSYRGEFQRFQMFYGPCEDEPILANQFSVFVSRPNGKKFSSVLCS 180
YHGVSLGGIGAGSIGRSYRGEFQRFQMFYGPCEDEP+LANQFSVFVSRPNG KFSSVLCS
Sbjct: 121 YHGVSLGGIGAGSIGRSYRGEFQRFQMFYGPCEDEPVLANQFSVFVSRPNGNKFSSVLCS 180
Query: 181 TKPQKSKDGKQDGIESWDWNLSGENSTYHALFPRSWTVYDGEPDPDLKIVCRQISPFIPH 240
KPQK KDG Q GI SWDWNLSGENSTYHALFPRSWT+Y+GEPDPDLKIVCRQISP IPH
Sbjct: 181 AKPQKFKDGNQTGIGSWDWNLSGENSTYHALFPRSWTIYNGEPDPDLKIVCRQISPIIPH 240
Query: 241 NYKESSFPVSVFTFSLFNEGQTSAQVTLLFTWAVSAANYFLFNFLYFIFITVNSVGGKSG 300
NYKESSFPVSVFTF+L NEGQTSAQVTLLFTWA NSVGGKSG
Sbjct: 241 NYKESSFPVSVFTFNLSNEGQTSAQVTLLFTWA-------------------NSVGGKSG 300
Query: 301 FTGHHFNSKIGAEDGAQGVLLHHKTANGRPSVTYAIAAEATDDVHVSLCPCFVISGESEG 360
FTGHHFNSK+ AEDGA GVLLHH+TA GRP+VTYAIAAEATDDVHVSLCPCFVISG+SEG
Sbjct: 301 FTGHHFNSKMRAEDGAHGVLLHHRTAGGRPTVTYAIAAEATDDVHVSLCPCFVISGDSEG 360
Query: 361 ISAKDMWQEIKNHGSFDKLGSVGAYEGSKPGSSIGAAIAATLTVPPRSARTVTFSLAWDC 420
ISAKDMWQEIKNHGSFDKLG+V A GSKPGSSIGAA+AATLT+PP SARTVTFSLAWDC
Sbjct: 361 ISAKDMWQEIKNHGSFDKLGNVAANGGSKPGSSIGAAVAATLTIPPASARTVTFSLAWDC 420
Query: 421 PEVKFDGKTYHR-----------------------------------------------Y 480
PEVKFDGKTYHR Y
Sbjct: 421 PEVKFDGKTYHRRYSKFYGTLGDAARTIAHDAILEHGKWEAEIEAWQRPIIEDKRLPEWY 480
Query: 481 PVTLFNELYFLNSGGTIWTDGLAPLQNLSTISDKKYFLERSKSHPNGGAPTADHRKDVAV 540
VTLFNELYFLNSGGTIWTDGL PLQNLSTIS KKYFLERSKS PNGG P HRKDVAV
Sbjct: 481 TVTLFNELYFLNSGGTIWTDGLPPLQNLSTISHKKYFLERSKSEPNGGVPNGVHRKDVAV 540
Query: 541 DILERMSQIFDQTHGGAGPSNAALGTRLLHPGEENVGNLLLVEGSQYLMWNTYDVHFYSS 600
DILERMSQIFDQT GGAGPSNAALGTRLLHPGEENVGNLLLVEGSQYLMWNTYDVHFYSS
Sbjct: 541 DILERMSQIFDQTDGGAGPSNAALGTRLLHPGEENVGNLLLVEGSQYLMWNTYDVHFYSS 600
Query: 601 FALIMLLPKLELSIQRDFAAAVLMHDPRKTKIMSDGNWVPRKVLGAVPHDIGFNDPWFEV 660
FALIML PKLELSIQRDFAAAVLMHDPRK KIMSDGNWVPRKVLGAVPHDIGFNDPWFEV
Sbjct: 601 FALIMLFPKLELSIQRDFAAAVLMHDPRKAKIMSDGNWVPRKVLGAVPHDIGFNDPWFEV 660
Query: 661 NAYNLLNVTRWKDLASKFVLQVYRDVVATGDKNFAQSVWPSVYVALAFMEQFDKDKDGMV 720
NAYNLLNVTRWKDL SKFVLQVYRDVVATGDKNFA+SVWPSVYVALAFMEQFDKDKDGM+
Sbjct: 661 NAYNLLNVTRWKDLGSKFVLQVYRDVVATGDKNFAKSVWPSVYVALAFMEQFDKDKDGMI 720
Query: 721 ENEGFPDQTYDTWTVKGVSAYCGGLWVAALQAASALASEVDDDAAAHYFWIKYQKARSVY 780
ENEGFPDQTYDTWTVKGVSAYCGGLWVAALQAASALASEVDD+AAAHYFWIKYQKARSVY
Sbjct: 721 ENEGFPDQTYDTWTVKGVSAYCGGLWVAALQAASALASEVDDEAAAHYFWIKYQKARSVY 780
Query: 781 DTLWNGSYFNYDNSKGPWSSSIQADQLAGQWYARACGLCPIADEEKIRSALEKIYNFN-- 840
+TLWNGSYFNYDNSKGPWSSSIQADQLAGQWYARACGLCPIADEEKIR ALEKIYNFN
Sbjct: 781 ETLWNGSYFNYDNSKGPWSSSIQADQLAGQWYARACGLCPIADEEKIRGALEKIYNFNVM 840
Query: 841 -VKGGTRGAVNGMFPDGRVDKSILQPKEIWAGVTYSVAASMIQEGLVETGFQTAMGIHQA 900
VKGGTRGAVNGMFPDG VDKSILQPKEIWAGVTYSVAA+MIQEGLVETGFQTAMG++ A
Sbjct: 841 KVKGGTRGAVNGMFPDGSVDKSILQPKEIWAGVTYSVAATMIQEGLVETGFQTAMGVYHA 900
Query: 901 AWAQDGLGYSFQTPEAWDVDDRYRSIGYMRPMAIWAMQWALMS-KKPTKIPTKALSKMEE 952
AWAQDGLGYSFQTPEAWDVDDR+RSI YMRP+AIWAMQWA+MS KPTK PTKA S+MEE
Sbjct: 901 AWAQDGLGYSFQTPEAWDVDDRFRSIAYMRPLAIWAMQWAMMSDSKPTKFPTKAFSEMEE 944
BLAST of Cla97C08G145420 vs. ExPASy Swiss-Prot
Match:
Q69ZF3 (Non-lysosomal glucosylceramidase OS=Mus musculus OX=10090 GN=Gba2 PE=1 SV=2)
HSP 1 Score: 496.9 bits (1278), Expect = 5.1e-139
Identity = 307/836 (36.72%), Postives = 434/836 (51.91%), Query Frame = 0
Query: 108 PLYEFFSDQPITCYHGVSLGGIGAGSIGRSYRGEFQRFQMFYGPCEDEPILANQFSVFVS 167
P + + P+ +G LGGIG G+I R +RG+F R+Q+ G + + ++A+QF V +
Sbjct: 127 PFIDMLNSLPLRQIYGCPLGGIGGGTITRGWRGQFCRWQLNPGMYQHQTVIADQFIVCLR 186
Query: 168 RPNGKKFSSVLCSTKPQKSKDGKQDGIESWDWNLSGENSTYHALFPRSWTVYDGEPDPDL 227
R + VL P + + SW+W L G + YHAL+PR+WTVY P ++
Sbjct: 187 RDGRTVYQQVLSLELP--------NVLRSWNWGLCGYFAFYHALYPRAWTVYQ-LPGQNV 246
Query: 228 KIVCRQISPFIPHNYKESSFPVSVFTFSLFNEGQTSAQVTLLFTWAVSAANYFLFNFLYF 287
+ CRQ++P +PH+Y++SS PV VF + + NEG + V++ F+
Sbjct: 247 TLTCRQVTPILPHDYQDSSLPVGVFVWDVENEGDETLDVSITFSMR-------------- 306
Query: 288 IFITVNSVGGKSGFTGHHFNSKIGAEDG---AQGVLLHHKTANGRPSVTYAIAAEATDDV 347
N +GG+ G +N E G QG+LLHH T T A+AA T D
Sbjct: 307 -----NGLGGEDDAAGSLWNEPFRLEQGGTTVQGLLLHHPTPPN--PYTMAVAARCTADT 366
Query: 348 HVSLCPCFVISGESEGISAKDMWQEIKNHGSFDKLGSVGAYEGSKPGSSIGAAIAATLTV 407
V+ F +G + + +WQ++ G D G ++ G I A+ + +
Sbjct: 367 TVTHTTAFDPNG-----TGQQVWQDLLQDGQLD--SPAGQSTPTQKGEGIAGAVCVSSKL 426
Query: 408 PPRSARTVTFSLAWDCPEVKFDGKT---YHRYPVTLFNELYFLNSGGTIWTDGLAPLQNL 467
PRS + FSLAWD P++ F K+ Y RY F S G + AP +
Sbjct: 427 LPRSRCCLEFSLAWDMPKIMFGAKSQVHYRRY-------TRFFGSDGDV-----APALSH 486
Query: 468 STISDKKYFLERSKSHPNGGAPTADHRKDVAVDILERMSQIFDQTHGG---------AGP 527
+ + +R + N P D R A ++++ GG + P
Sbjct: 487 YALCHYADWEDRISAWQN---PVLDDRTLPAWYKSALFNELYFLADGGTVWLEVPADSLP 546
Query: 528 SNAALGTRLLHPGEENVGNLLLVEGSQYLMWNTYDVHFYSSFALIMLLPKLELSIQRDFA 587
R L ++ G +EG +Y M+NTYDVHFY+SFAL+ML PKLELS+Q D A
Sbjct: 547 EGLGGSMRQLRSTLQDYGRFGYLEGQEYRMYNTYDVHFYASFALVMLWPKLELSLQYDMA 606
Query: 588 AAVLMHDPRKTKIMSDGNWVPRKVLGAVPHDIGFND--PWFEVNAYNLLNVTRWKDLASK 647
A L D + + + G P K +PHDIG D PW VNAY + + WKDL K
Sbjct: 607 LATLKEDLTRRRYLMSGVVAPVKRRNVIPHDIGDPDDEPWLRVNAYLIHDTADWKDLNLK 666
Query: 648 FVLQVYRDVVATGDKNFAQSVWPSVYVALAFME---QFDKDKDGMVENEGFPDQTYDTWT 707
FVLQ+YRD TGD+ F + +WP V LA ME +FDKD+DG++EN G+ DQTYD W
Sbjct: 667 FVLQIYRDYYLTGDQGFLEDMWP---VCLAVMESEMKFDKDQDGLIENGGYADQTYDAWV 726
Query: 708 VKGVSAYCGGLWVAALQAASALASEVDDDAAAHYFWIKYQKARSVYD-TLWNGSYFNYDN 767
G SAYCGGLW+AA+ +A F + R Y+ LWNG Y+NYD+
Sbjct: 727 TTGPSAYCGGLWLAAVAVMVQMAVLCGAQDVQERFASILCRGREAYERLLWNGRYYNYDS 786
Query: 768 SKGPWSSSIQADQLAGQWYARACGL----CPIADEEKIRSALEKIYNFNVK---GGTRGA 827
S P S SI +DQ AGQW+ RACGL + + AL+ I+ NV+ GG GA
Sbjct: 787 SSHPQSRSIMSDQCAGQWFLRACGLGEGDTEVFPTLHVVRALQTIFELNVQAFAGGAMGA 846
Query: 828 VNGMFPDGRVDKSILQPKEIWAGVTYSVAASMIQEGLVETGFQTAMGIHQAAWAQDGLGY 887
VNGM P G D+S +Q E+W GV Y +AA+MIQEGL GF+TA G ++ W + LG
Sbjct: 847 VNGMHPHGVPDRSSVQSDEVWVGVVYGLAATMIQEGLTWEGFRTAEGCYRTVW--ERLGL 903
Query: 888 SFQTPEAWDVDDRYRSIGYMRPMAIWAMQWALMSKKPTKIPTKALSKMEETAFATQ 916
+FQTPEA+ +RS+ YMRP++IWAMQ AL ++ K ++ S + T +TQ
Sbjct: 907 AFQTPEAYCQQQVFRSLAYMRPLSIWAMQLALQQQQHKK--SRRPSVTQGTGLSTQ 903
BLAST of Cla97C08G145420 vs. ExPASy Swiss-Prot
Match:
Q9HCG7 (Non-lysosomal glucosylceramidase OS=Homo sapiens OX=9606 GN=GBA2 PE=1 SV=2)
HSP 1 Score: 486.9 bits (1252), Expect = 5.3e-136
Identity = 302/819 (36.87%), Postives = 425/819 (51.89%), Query Frame = 0
Query: 108 PLYEFFSDQPITCYHGVSLGGIGAGSIGRSYRGEFQRFQMFYGPCEDEPILANQFSVFVS 167
P + + P+ +G LGGIG G+I R +RG+F R+Q+ G + ++A+QF+V +
Sbjct: 136 PFIDMINSVPLRQIYGCPLGGIGGGTITRGWRGQFCRWQLNPGMYQHRTVIADQFTVCLR 195
Query: 168 RPNGKKFSSVLCSTKPQKSKDGKQDGIESWDWNLSGENSTYHALFPRSWTVYDGEPDPDL 227
R + VL +P + SW+W L G + YHAL+PR+WTVY P ++
Sbjct: 196 REGQTVYQQVLSLERPSV--------LRSWNWGLCGYFAFYHALYPRAWTVYQ-LPGQNV 255
Query: 228 KIVCRQISPFIPHNYKESSFPVSVFTFSLFNEGQTSAQVTLLFTWAVSAANYFLFNFLYF 287
+ CRQI+P +PH+Y++SS PV VF + + NEG + V+++F+
Sbjct: 256 TLTCRQITPILPHDYQDSSLPVGVFVWDVENEGDEALDVSIMFSMR-------------- 315
Query: 288 IFITVNSVGGKSGFTGHHFNSKIGAE---DGAQGVLLHHKTANGRPSVTYAIAAEATDDV 347
N +GG G +N E + +G+LLHH T T A+AA T
Sbjct: 316 -----NGLGGGDDAPGGLWNEPFCLERSGETVRGLLLHHPTLPN--PYTMAVAARVTAAT 375
Query: 348 HVSLCPCFVISGESEGISAKDMWQEIKNHGSFDKLGSVGAYEGSKPGSSIGAAIAATLTV 407
V+ F +S G + +WQ++ G D G ++ G I A+ + +
Sbjct: 376 TVTHITAF--DPDSTG---QQVWQDLLQDGQLD--SPTGQSTPTQKGVGIAGAVCVSSKL 435
Query: 408 PPRSARTVTFSLAWDCPEVKF--DGKTYHRYPVTLFNELYFLNSGGTIWTDG-LAPLQNL 467
PR + FSLAWD P + F G+ ++R F + DG AP +
Sbjct: 436 RPRGQCRLEFSLAWDMPRIMFGAKGQVHYRRYTRFFGQ------------DGDAAPALSH 495
Query: 468 STISDKKYFLERSKSHPNGGAPTADHRKDVAVDILERMSQIFDQTHGGA-------GPSN 527
+ + ER + +P D R A ++++ GG
Sbjct: 496 YALCRYAEWEERISAWQ---SPVLDDRSLPAWYKSALFNELYFLADGGTVWLEVLEDSLP 555
Query: 528 AALGTRLLH--PGEENVGNLLLVEGSQYLMWNTYDVHFYSSFALIMLLPKLELSIQRDFA 587
LG + H P + G +EG +Y M+NTYDVHFY+SFALIML PKLELS+Q D A
Sbjct: 556 EELGRNMCHLRPTLRDYGRFGYLEGQEYRMYNTYDVHFYASFALIMLWPKLELSLQYDMA 615
Query: 588 AAVLMHDPRKTKIMSDGNWVPRKVLGAVPHDIGFND--PWFEVNAYNLLNVTRWKDLASK 647
A L D + + + G P K +PHDIG D PW VNAY + + WKDL K
Sbjct: 616 LATLREDLTRRRYLMSGVMAPVKRRNVIPHDIGDPDDEPWLRVNAYLIHDTADWKDLNLK 675
Query: 648 FVLQVYRDVVATGDKNFAQSVWPSVYVALAFME---QFDKDKDGMVENEGFPDQTYDTWT 707
FVLQVYRD TGD+NF + +WP V LA ME +FDKD DG++EN G+ DQTYD W
Sbjct: 676 FVLQVYRDYYLTGDQNFLKDMWP---VCLAVMESEMKFDKDHDGLIENGGYADQTYDGWV 735
Query: 708 VKGVSAYCGGLWVAALQAASALASEVDDDAAAHYFWIKYQKARSVYD-TLWNGSYFNYDN 767
G SAYCGGLW+AA+ +A+ F + + Y+ LWNG Y+NYD+
Sbjct: 736 TTGPSAYCGGLWLAAVAVMVQMAALCGAQDIQDKFSSILSRGQEAYERLLWNGRYYNYDS 795
Query: 768 SKGPWSSSIQADQLAGQWYARACGL----CPIADEEKIRSALEKIYNFNVK---GGTRGA 827
S P S S+ +DQ AGQW+ +ACGL + + + AL+ I+ NV+ GG GA
Sbjct: 796 SSRPQSRSVMSDQCAGQWFLKACGLGEGDTEVFPTQHVVRALQTIFELNVQAFAGGAMGA 855
Query: 828 VNGMFPDGRVDKSILQPKEIWAGVTYSVAASMIQEGLVETGFQTAMGIHQAAWAQDGLGY 887
VNGM P G DKS +Q E+W GV Y +AA+MIQEGL GFQTA G ++ W + LG
Sbjct: 856 VNGMQPHGVPDKSSVQSDEVWVGVVYGLAATMIQEGLTWEGFQTAEGCYRTVW--ERLGL 897
Query: 888 SFQTPEAWDVDDRYRSIGYMRPMAIWAMQWALMSKKPTK 899
+FQTPEA+ +RS+ YMRP++IWAMQ AL ++ K
Sbjct: 916 AFQTPEAYCQQRVFRSLAYMRPLSIWAMQLALQQQQHKK 897
BLAST of Cla97C08G145420 vs. ExPASy Swiss-Prot
Match:
Q5M868 (Non-lysosomal glucosylceramidase OS=Rattus norvegicus OX=10116 GN=Gba2 PE=2 SV=2)
HSP 1 Score: 486.5 bits (1251), Expect = 6.9e-136
Identity = 304/819 (37.12%), Postives = 423/819 (51.65%), Query Frame = 0
Query: 108 PLYEFFSDQPITCYHGVSLGGIGAGSIGRSYRGEFQRFQMFYGPCEDEPILANQFSVFVS 167
P + F+ P+ +G LGGIG G+I R +RG+F R+Q+ G + + ++A+QF V +
Sbjct: 127 PFIDMFNSVPLRQIYGCPLGGIGGGTITRGWRGQFCRWQLNPGMYQHQTVIADQFIVCLR 186
Query: 168 RPNGKKFSSVLCSTKPQKSKDGKQDGIESWDWNLSGENSTYHALFPRSWTVYDGEPDPDL 227
R + VL P + SW+W L G + YHAL+PR+WTVY P ++
Sbjct: 187 RDGRTVYQQVLSLELPSV--------LRSWNWGLCGYFAFYHALYPRAWTVYQ-LPGQNV 246
Query: 228 KIVCRQISPFIPHNYKESSFPVSVFTFSLFNEGQTSAQVTLLFTWAVSAANYFLFNFLYF 287
+ CRQI+P +PH+Y++SS PV VF + + NEG + V+++F+
Sbjct: 247 TLTCRQITPILPHDYQDSSLPVGVFVWDVENEGDETLDVSIMFSMR-------------- 306
Query: 288 IFITVNSVGGKSGFTGHHFNSKIGAE-DG--AQGVLLHHKTANGRPSVTYAIAAEATDDV 347
N +GG+ G +N E DG QG+LLHH T T A+AA T D
Sbjct: 307 -----NGLGGEDDAAGGLWNEPFRLEQDGTTVQGLLLHHPTPPN--PYTMAVAARHTADT 366
Query: 348 HVSLCPCFVISGESEGISAKDMWQEIKNHGSFDKLGSVGAYEGSKPGSSIGAAIAATLTV 407
V+ F +S G + +WQ++ G D G ++ G + A+ A+ +
Sbjct: 367 TVTYTTAF--DPDSTG---QQVWQDLLQDGQLD--SPAGQSTPTQRGEGVAGAVCASSKL 426
Query: 408 PPRSARTVTFSLAWDCPEVKFDGK---TYHRYPVTLFNELYFLNSGGTIWTDGLAPLQNL 467
PR + FSLAWD P + F K Y RY F S G + AP +
Sbjct: 427 LPRGRCCLEFSLAWDMPRIMFGAKGQVHYRRY-------TRFFGSDGDV-----APALSH 486
Query: 468 STISDKKYFLERSKSHPNGGAPTADHRKDVAVDILERMSQIFDQTHGG---------AGP 527
+ + + N P D R A ++++ GG + P
Sbjct: 487 YALCQYAGWENSISAWQN---PVLDDRSLPAWYKSALFNELYFLADGGTVWLEVPEDSLP 546
Query: 528 SNAALGTRLLHPGEENVGNLLLVEGSQYLMWNTYDVHFYSSFALIMLLPKLELSIQRDFA 587
L P ++ G +EG +Y M+NTYDVHFY+SFAL+ML PKLELS+Q D A
Sbjct: 547 EELGGSMYQLRPILQDYGRFGYLEGQEYRMYNTYDVHFYASFALVMLWPKLELSLQYDMA 606
Query: 588 AAVLMHDPRKTKIMSDGNWVPRKVLGAVPHDIGFND--PWFEVNAYNLLNVTRWKDLASK 647
A D + + + G P K +PHDIG D PW VNAY + + WKDL K
Sbjct: 607 LATFKEDLTRRRYLMSGVVAPVKRRNVIPHDIGDPDDEPWLRVNAYLIHDTADWKDLNLK 666
Query: 648 FVLQVYRDVVATGDKNFAQSVWPSVYVALAFME---QFDKDKDGMVENEGFPDQTYDTWT 707
FVLQVYRD TGD+ F + +WP V LA ME +FDKD+DG++EN G+ DQTYD W
Sbjct: 667 FVLQVYRDYYLTGDQGFLKDMWP---VCLAVMESEMKFDKDQDGLIENGGYADQTYDGWV 726
Query: 708 VKGVSAYCGGLWVAALQAASALASEVDDDAAAHYFWIKYQKARSVYD-TLWNGSYFNYDN 767
G SAYCGGLW+AA+ +A F + R Y+ LWNG Y+NYD+
Sbjct: 727 TTGPSAYCGGLWLAAVAVMVQMAVLCGAQDVQDKFSSILCRGREAYERLLWNGRYYNYDS 786
Query: 768 SKGPWSSSIQADQLAGQWYARACGL----CPIADEEKIRSALEKIYNFNVK---GGTRGA 827
S P S S+ +DQ AGQW+ RACGL + + AL+ I+ NV+ GG GA
Sbjct: 787 SSQPQSRSVMSDQCAGQWFLRACGLGEGDTEVFPTLHVVRALKTIFELNVQAFAGGAMGA 846
Query: 828 VNGMFPDGRVDKSILQPKEIWAGVTYSVAASMIQEGLVETGFQTAMGIHQAAWAQDGLGY 887
VNGM P G D+S +Q E+W GV Y +AA+MIQEGL GF+TA G ++ W + LG
Sbjct: 847 VNGMQPHGVPDRSSVQSDEVWVGVVYGLAATMIQEGLTWEGFRTAEGCYRTVW--ERLGL 888
Query: 888 SFQTPEAWDVDDRYRSIGYMRPMAIWAMQWALMSKKPTK 899
+FQTPEA+ +RS+ YMRP++IWAMQ AL ++ K
Sbjct: 907 AFQTPEAYCQQRVFRSLAYMRPLSIWAMQLALQQQQHKK 888
BLAST of Cla97C08G145420 vs. ExPASy Swiss-Prot
Match:
Q7KT91 (Non-lysosomal glucosylceramidase OS=Drosophila melanogaster OX=7227 GN=CG33090 PE=1 SV=1)
HSP 1 Score: 424.1 bits (1089), Expect = 4.2e-117
Identity = 294/877 (33.52%), Postives = 430/877 (49.03%), Query Frame = 0
Query: 122 HGVSLGGIGAGSIGRSYRGEFQRFQMFYGPCEDEPILANQFSVFVSRPNG-KKFSSVL-- 181
+GV +GGIG G+IGR Y GEF RFQM G E +LANQF V + P G F S+L
Sbjct: 116 YGVPIGGIGGGTIGRGYAGEFCRFQMRPGIYEYNVVLANQFIVTIKDPKGCTIFQSLLSK 175
Query: 182 CSTKPQKS-KDGKQDG-----------------IESWDWNLSGENSTYHALFPRSWTVYD 241
CST+ + S DG DG + +W N+ +Y L+PRSWT YD
Sbjct: 176 CSTRDKTSDPDGDPDGERTKCQLPNCSSRAKQPLSAWHSNIEDTRCSYTGLYPRSWTEYD 235
Query: 242 GEPDPDLKIVCRQISPFIPHNYKESSFPVSVFTFSLFNEGQTSAQVTLLFTWAVSAANYF 301
+++ CRQ+SP IPH Y+ESS P +VF +S+ N +V++ FT+
Sbjct: 236 -LSHYGVRLTCRQVSPVIPHEYRESSLPCAVFVWSVENVCDQERKVSITFTFK------- 295
Query: 302 LFNFLYFIFITVNSVGGKSGFTGHHFNSKIGAEDGAQGVLLHHKTANGRPSVTYAIAAEA 361
N G K S++ +E A+GV + K + +Y +A
Sbjct: 296 ------------NGTGNKKQDAEGGAESQLISEGNAKGVSIRQKIS--EMPCSYNLACRV 355
Query: 362 TDDVHVSLCPCFVISGESEGISAKDMWQEIKNHGSFDKLGSVGAYEGSKPGSSIGAAIAA 421
++ ++ CP F +G E +W ++K HG + + A + IG A+
Sbjct: 356 LPEISITRCPQFDPAGNGE-----QLWAQLKEHGQLSEHPTSEALK----TKDIGVAVCG 415
Query: 422 TLTVPPRSARTVTFSLAWDCPEVKFDGK--TYHRYPVTLFNELYFLNSGGTIWTDGLAPL 481
+ + P ++ + F LAWD P+++F K T+ RY F++ +SG I L
Sbjct: 416 QVALKPMASHDLEFVLAWDMPKIQFPRKMQTHTRYYTKYFDDSG--DSGPRICEYALRQY 475
Query: 482 QNLSTISDKKYFLERSKSHPNGGAPTADHRKDVAVDILERMSQIFDQTHGGA--GPSNAA 541
+ D + P T A+ +Q++ + GG +++
Sbjct: 476 STWERLID-------AWQRPILNDETLPDWYKCAI-----FNQLYFISDGGTIWLKCDSS 535
Query: 542 LGTRLLHPGEE-NVGNLLLVEGSQYLMWNTYDVHFYSSFALIMLLPKLELSIQRDFAAAV 601
LG L + G +EG +Y M+NTYDVHFY+S AL L P L++S+Q DF A+
Sbjct: 536 LGKELAYDDPRLAYGRFGYLEGHEYRMYNTYDVHFYASPALAHLWPNLQVSLQYDFKDAI 595
Query: 602 LMHDPRKTKIMSDGNWVPRKVLGAVPHDIGFND--PWFEVNAYNLLNVTRWKDLASKFVL 661
K++ DG +PRKV VPHD+G D P+ +N YN+ +V WKDL +KFVL
Sbjct: 596 AAELNDTRKMLYDGKVMPRKVKNCVPHDLGDPDEEPFTLINCYNIHDVNDWKDLNTKFVL 655
Query: 662 QVYRDVVATGDKNFAQSVWPSVYVALAFME------------------------------ 721
QVYRD + AQS S + ++ F++
Sbjct: 656 QVYRDYYVLNELAQAQSDNASKFSSIEFIDKESLYELYSQDNKRKNSADEKQQNRKSASM 715
Query: 722 ---------------------------------QFDKDKDGMVENEGFPDQTYDTWTVKG 781
++DKD DG++EN PDQTYD+W + G
Sbjct: 716 YINETNGKVYLMDAIGYLKAMYASCKAIMERTIEYDKDNDGLIENTKMPDQTYDSWVMDG 775
Query: 782 VSAYCGGLWVAALQAASALASEVDD-DAAAHYFWIKYQKARSVYDTLWNGSYFNYDNSKG 841
SAYC GLW+AALQA SA+A+ +D + Y I + RS+ + LWNGSY+ +D S
Sbjct: 776 PSAYCSGLWLAALQAMSAMATILDQPNDCLRYQDILEKGKRSLEEKLWNGSYYRFDLSHS 835
Query: 842 PWSSSIQADQLAGQWYARACGL-CPIADEEKIRSALEKIYNFNVKG---GTRGAVNGMFP 896
+I ADQL G WY ++CG I +E +R+AL++IY+ NV G G GA NG
Sbjct: 836 -HRDTIMADQLCGHWYLKSCGFDYEIYPKENVRTALKRIYDNNVMGFHEGNIGAANGFIA 895
BLAST of Cla97C08G145420 vs. ExPASy TrEMBL
Match:
A0A0A0KXW4 (Non-lysosomal glucosylceramidase OS=Cucumis sativus OX=3659 GN=Csa_5G649350 PE=3 SV=1)
HSP 1 Score: 1664.8 bits (4310), Expect = 0.0e+00
Identity = 828/1003 (82.55%), Postives = 857/1003 (85.44%), Query Frame = 0
Query: 1 MEKGGNGASSTEVDPSKPPSLTWKRKLNFTGKSPESFSFTLTDAWHLGSLGYRLWRHGKE 60
MEKGGNGASSTEVDPSKPPSLTWKRKL+FTGKSPESFSFTLTDAWH+G GYRLWR+GKE
Sbjct: 1 MEKGGNGASSTEVDPSKPPSLTWKRKLDFTGKSPESFSFTLTDAWHMGMTGYRLWRNGKE 60
Query: 61 EAAKGRVIMTASANFFQIDSVFLFHLIIVSLYSIFSLSLSLSLCPQIPLYEFFSDQPITC 120
E AKGR IP+YEFFSD PITC
Sbjct: 61 EIAKGR----------------------------------------IPIYEFFSDVPITC 120
Query: 121 YHGVSLGGIGAGSIGRSYRGEFQRFQMFYGPCEDEPILANQFSVFVSRPNGKKFSSVLCS 180
YHGVSLGGIGAGSIGRSYRGEFQRFQMFYGPCEDEP+LANQFSVFVSRPNG KFSSVLCS
Sbjct: 121 YHGVSLGGIGAGSIGRSYRGEFQRFQMFYGPCEDEPVLANQFSVFVSRPNGNKFSSVLCS 180
Query: 181 TKPQKSKDGKQDGIESWDWNLSGENSTYHALFPRSWTVYDGEPDPDLKIVCRQISPFIPH 240
KPQKSKDGKQ GIESWDWNLSGENSTYHALFPRSWTVYDGEPDPDLKIVCRQ+SP IPH
Sbjct: 181 AKPQKSKDGKQTGIESWDWNLSGENSTYHALFPRSWTVYDGEPDPDLKIVCRQMSPIIPH 240
Query: 241 NYKESSFPVSVFTFSLFNEGQTSAQVTLLFTWAVSAANYFLFNFLYFIFITVNSVGGKSG 300
NYKESSFPVSVFTF L NEGQTSAQVTLLFTWA NSVGGKSG
Sbjct: 241 NYKESSFPVSVFTFKLSNEGQTSAQVTLLFTWA-------------------NSVGGKSG 300
Query: 301 FTGHHFNSKIGAEDGAQGVLLHHKTANGRPSVTYAIAAEATDDVHVSLCPCFVISGESEG 360
FTGHHFNSK+GAEDGAQGVLLHHK+ANGRP+VTY IAAEATDDVHVSLCPCFVISG+SEG
Sbjct: 301 FTGHHFNSKMGAEDGAQGVLLHHKSANGRPTVTYGIAAEATDDVHVSLCPCFVISGDSEG 360
Query: 361 ISAKDMWQEIKNHGSFDKLGSVGAYEGSKPGSSIGAAIAATLTVPPRSARTVTFSLAWDC 420
ISAKDMWQEIKNHGSFD LGSVGA EGSKPG SIGAA+AATLT+PP SARTVTFSLAWDC
Sbjct: 361 ISAKDMWQEIKNHGSFDNLGSVGANEGSKPGCSIGAAVAATLTIPPTSARTVTFSLAWDC 420
Query: 421 PEVKFDGKTYHR-----------------------------------------------Y 480
PEVKFDGKTYHR Y
Sbjct: 421 PEVKFDGKTYHRQYSKFYGTLGDAAEIIARDAISKHGKWEAEIEAWQRPIIEDKRLPDWY 480
Query: 481 PVTLFNELYFLNSGGTIWTDGLAPLQNLSTISDKKYFLERSKSHPNGGAPTADHRKDVAV 540
PVTLFNELYFLNSGGTIWTDGL PLQNLSTIS KKYFLERSKS NGGAP DHRKDVAV
Sbjct: 481 PVTLFNELYFLNSGGTIWTDGLPPLQNLSTISHKKYFLERSKSELNGGAPNGDHRKDVAV 540
Query: 541 DILERMSQIFDQTHGGAGPSNAALGTRLLHPGEENVGNLLLVEGSQYLMWNTYDVHFYSS 600
DILERMSQI DQTHGGAGPSNAALGTRLLHPGEENVGNLLLVEGSQYLMWNTYDVHFYSS
Sbjct: 541 DILERMSQILDQTHGGAGPSNAALGTRLLHPGEENVGNLLLVEGSQYLMWNTYDVHFYSS 600
Query: 601 FALIMLLPKLELSIQRDFAAAVLMHDPRKTKIMSDGNWVPRKVLGAVPHDIGFNDPWFEV 660
FALIML PKLELSIQRDFAAAVLMHDPRK K M DGNWVPRKVLGAVPHDIGFNDPW EV
Sbjct: 601 FALIMLFPKLELSIQRDFAAAVLMHDPRKAKTMCDGNWVPRKVLGAVPHDIGFNDPWLEV 660
Query: 661 NAYNLLNVTRWKDLASKFVLQVYRDVVATGDKNFAQSVWPSVYVALAFMEQFDKDKDGMV 720
NAYNLLNV+RWKDL SKFVLQVYRDVVATGDKNFA+SVWPSVYVALAFMEQFDKDKDGM+
Sbjct: 661 NAYNLLNVSRWKDLGSKFVLQVYRDVVATGDKNFAKSVWPSVYVALAFMEQFDKDKDGMI 720
Query: 721 ENEGFPDQTYDTWTVKGVSAYCGGLWVAALQAASALASEVDDDAAAHYFWIKYQKARSVY 780
ENEGFPDQTYDTWTVKGVSAYCGGLWVAALQAASALASEVDD+AAAHYFWIKYQKARSVY
Sbjct: 721 ENEGFPDQTYDTWTVKGVSAYCGGLWVAALQAASALASEVDDEAAAHYFWIKYQKARSVY 780
Query: 781 DTLWNGSYFNYDNSKGPWSSSIQADQLAGQWYARACGLCPIADEEKIRSALEKIYNFN-- 840
+TLWNGSYFNYDNSKGPWSSSIQADQLAGQWYARACGLCPIADEEKIR ALEKIYNFN
Sbjct: 781 ETLWNGSYFNYDNSKGPWSSSIQADQLAGQWYARACGLCPIADEEKIRVALEKIYNFNVM 840
Query: 841 -VKGGTRGAVNGMFPDGRVDKSILQPKEIWAGVTYSVAASMIQEGLVETGFQTAMGIHQA 900
VKGGTRGAVNGMFPDG VDKSILQPKEIWAGVTYSVAA+MIQEG+VETGFQTAMGI+QA
Sbjct: 841 KVKGGTRGAVNGMFPDGSVDKSILQPKEIWAGVTYSVAATMIQEGMVETGFQTAMGIYQA 900
Query: 901 AWAQDGLGYSFQTPEAWDVDDRYRSIGYMRPMAIWAMQWALMS-KKPTKIPTKALSKMEE 952
AWAQDGLGYSFQTPEAWDVDDR+RSIGYMRP+AIWAMQWA+MS KPTK+PTKA S+M+E
Sbjct: 901 AWAQDGLGYSFQTPEAWDVDDRFRSIGYMRPLAIWAMQWAMMSDSKPTKVPTKAFSEMQE 944
BLAST of Cla97C08G145420 vs. ExPASy TrEMBL
Match:
A0A5A7T726 (Non-lysosomal glucosylceramidase OS=Cucumis melo var. makuwa OX=1194695 GN=E6C27_scaffold64G00770 PE=3 SV=1)
HSP 1 Score: 1654.8 bits (4284), Expect = 0.0e+00
Identity = 823/994 (82.80%), Postives = 852/994 (85.71%), Query Frame = 0
Query: 1 MEKGGNGASSTEVDPSKPPSLTWKRKLNFTGKSPESFSFTLTDAWHLGSLGYRLWRHGKE 60
MEKGGNGASSTEVDPSKPPSLTWKRKL+FTGKSPE FSFTLTDAWH+G LGYRLWR+GKE
Sbjct: 1 MEKGGNGASSTEVDPSKPPSLTWKRKLDFTGKSPECFSFTLTDAWHMGMLGYRLWRNGKE 60
Query: 61 EAAKGRVIMTASANFFQIDSVFLFHLIIVSLYSIFSLSLSLSLCPQIPLYEFFSDQPITC 120
E AKGR IP+YEFFSDQPITC
Sbjct: 61 EIAKGR----------------------------------------IPIYEFFSDQPITC 120
Query: 121 YHGVSLGGIGAGSIGRSYRGEFQRFQMFYGPCEDEPILANQFSVFVSRPNGKKFSSVLCS 180
YHGVSLGGIGAGSIGRSYRGEFQRFQMFYGPCEDEP+LANQFSVFVSRPNG KFSSVLCS
Sbjct: 121 YHGVSLGGIGAGSIGRSYRGEFQRFQMFYGPCEDEPVLANQFSVFVSRPNGNKFSSVLCS 180
Query: 181 TKPQKSKDGKQDGIESWDWNLSGENSTYHALFPRSWTVYDGEPDPDLKIVCRQISPFIPH 240
KPQK KDG Q GI SWDWNLSGENSTYHALFPRSWT+Y+GEPDPDLKIVCRQISP IPH
Sbjct: 181 AKPQKFKDGNQTGIGSWDWNLSGENSTYHALFPRSWTIYNGEPDPDLKIVCRQISPIIPH 240
Query: 241 NYKESSFPVSVFTFSLFNEGQTSAQVTLLFTWAVSAANYFLFNFLYFIFITVNSVGGKSG 300
NYKESSFPVSVFTF+L NEGQTSAQVTLLFTWA NSVGGKSG
Sbjct: 241 NYKESSFPVSVFTFNLSNEGQTSAQVTLLFTWA-------------------NSVGGKSG 300
Query: 301 FTGHHFNSKIGAEDGAQGVLLHHKTANGRPSVTYAIAAEATDDVHVSLCPCFVISGESEG 360
FTGHHFNSK+ AEDGA GVLLHH+TA GRP+VTYAIAAEATDDVHVSLCPCFVISG+SEG
Sbjct: 301 FTGHHFNSKMRAEDGAHGVLLHHRTAGGRPTVTYAIAAEATDDVHVSLCPCFVISGDSEG 360
Query: 361 ISAKDMWQEIKNHGSFDKLGSVGAYEGSKPGSSIGAAIAATLTVPPRSARTVTFSLAWDC 420
ISAKDMWQEIKNHGSFDKLG+V A GSKPGSSIGAA+AATLT+PP SARTVTFSLAWDC
Sbjct: 361 ISAKDMWQEIKNHGSFDKLGNVAANGGSKPGSSIGAAVAATLTIPPASARTVTFSLAWDC 420
Query: 421 PEVKFDGKTYHR--------------------------------------YPVTLFNELY 480
PEVKFDGKTYHR Y VTLFNELY
Sbjct: 421 PEVKFDGKTYHRSYTKFYGTLGDAARTIAHDAILEAWQRPIIEDKRLPEWYAVTLFNELY 480
Query: 481 FLNSGGTIWTDGLAPLQNLSTISDKKYFLERSKSHPNGGAPTADHRKDVAVDILERMSQI 540
FLNSGGTIWTDGL PLQNLSTIS KKYFLERSKS PNGG P HRKDVAVDILERMSQI
Sbjct: 481 FLNSGGTIWTDGLPPLQNLSTISHKKYFLERSKSEPNGGVPNGVHRKDVAVDILERMSQI 540
Query: 541 FDQTHGGAGPSNAALGTRLLHPGEENVGNLLLVEGSQYLMWNTYDVHFYSSFALIMLLPK 600
FDQT GGAGPSNAALGTRLLHPGEENVGNLLLVEGSQYLMWNTYDVHFYSSFALIML PK
Sbjct: 541 FDQTDGGAGPSNAALGTRLLHPGEENVGNLLLVEGSQYLMWNTYDVHFYSSFALIMLFPK 600
Query: 601 LELSIQRDFAAAVLMHDPRKTKIMSDGNWVPRKVLGAVPHDIGFNDPWFEVNAYNLLNVT 660
LELSIQRDFAAAVLMHDPRK KIMSDGNWVPRKVLGAVPHDIGFNDPWFEVNAYNLLNVT
Sbjct: 601 LELSIQRDFAAAVLMHDPRKAKIMSDGNWVPRKVLGAVPHDIGFNDPWFEVNAYNLLNVT 660
Query: 661 RWKDLASKFVLQVYRDVVATGDKNFAQSVWPSVYVALAFMEQFDKDKDGMVENEGFPDQT 720
RWKDL SKFVLQVYRDVVATGDKNFA+SVWPSVYVALAFMEQFDKDKDGM+ENEGFPDQT
Sbjct: 661 RWKDLGSKFVLQVYRDVVATGDKNFAKSVWPSVYVALAFMEQFDKDKDGMIENEGFPDQT 720
Query: 721 YDTWTVKGVSAYCGGLWVAALQAASALASEVDDDAAAHYFWIKYQKARSVYDTLWNGSYF 780
YDTWTVKGVSAYCGGLWVAALQAASALASEVDD+AAAHYFWIKYQKARSVY+TLWNGSYF
Sbjct: 721 YDTWTVKGVSAYCGGLWVAALQAASALASEVDDEAAAHYFWIKYQKARSVYETLWNGSYF 780
Query: 781 NYDNSKGPWSSSIQADQLAGQWYARACGLCPIADEEKIRSALEKIYNFN---VKGGTRGA 840
NYDNSKGPWSSSIQADQLAGQWYARACGLCPIADEEKIR ALEKIYNFN VKGGTRGA
Sbjct: 781 NYDNSKGPWSSSIQADQLAGQWYARACGLCPIADEEKIRGALEKIYNFNVMKVKGGTRGA 840
Query: 841 VNGMFPDGRVDKSILQPKEIWAGVTYSVAASMIQEGLVETGFQTAMGIHQAAWAQDGLGY 900
VNGMFPDG VDKSILQPKEIWAGVTYSVAA+MIQEGLVETGFQTAMG++ AAWAQDGLGY
Sbjct: 841 VNGMFPDGSVDKSILQPKEIWAGVTYSVAATMIQEGLVETGFQTAMGVYHAAWAQDGLGY 900
Query: 901 SFQTPEAWDVDDRYRSIGYMRPMAIWAMQWALMS-KKPTKIPTKALSKMEETAFATQHAA 952
SFQTPEAWDVDDR+RSI YMRP+AIWAMQWA+MS KPTK PTKA S+MEE+AFATQH A
Sbjct: 901 SFQTPEAWDVDDRFRSIAYMRPLAIWAMQWAMMSDSKPTKFPTKAFSEMEESAFATQHTA 935
BLAST of Cla97C08G145420 vs. ExPASy TrEMBL
Match:
A0A5D3BQT4 (Non-lysosomal glucosylceramidase OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold169G00760 PE=3 SV=1)
HSP 1 Score: 1654.8 bits (4284), Expect = 0.0e+00
Identity = 823/994 (82.80%), Postives = 852/994 (85.71%), Query Frame = 0
Query: 1 MEKGGNGASSTEVDPSKPPSLTWKRKLNFTGKSPESFSFTLTDAWHLGSLGYRLWRHGKE 60
MEKGGNGASSTEVDPSKPPSLTWKRKL+FTGKSPE FSFTLTDAWH+G LGYRLWR+GKE
Sbjct: 1 MEKGGNGASSTEVDPSKPPSLTWKRKLDFTGKSPECFSFTLTDAWHMGMLGYRLWRNGKE 60
Query: 61 EAAKGRVIMTASANFFQIDSVFLFHLIIVSLYSIFSLSLSLSLCPQIPLYEFFSDQPITC 120
E AKGR IP+YEFFSDQPITC
Sbjct: 61 EIAKGR----------------------------------------IPIYEFFSDQPITC 120
Query: 121 YHGVSLGGIGAGSIGRSYRGEFQRFQMFYGPCEDEPILANQFSVFVSRPNGKKFSSVLCS 180
YHGVSLGGIGAGSIGRSYRGEFQRFQMFYGPCEDEP+LANQFSVFVSRPNG KFSSVLCS
Sbjct: 121 YHGVSLGGIGAGSIGRSYRGEFQRFQMFYGPCEDEPVLANQFSVFVSRPNGNKFSSVLCS 180
Query: 181 TKPQKSKDGKQDGIESWDWNLSGENSTYHALFPRSWTVYDGEPDPDLKIVCRQISPFIPH 240
KPQK KDG Q GI SWDWNLSGENSTYHALFPRSWT+Y+GEPDPDLKIVCRQISP IPH
Sbjct: 181 AKPQKFKDGNQTGIGSWDWNLSGENSTYHALFPRSWTIYNGEPDPDLKIVCRQISPIIPH 240
Query: 241 NYKESSFPVSVFTFSLFNEGQTSAQVTLLFTWAVSAANYFLFNFLYFIFITVNSVGGKSG 300
NYKESSFPVSVFTF+L NEGQTSAQVTLLFTWA NSVGGKSG
Sbjct: 241 NYKESSFPVSVFTFNLSNEGQTSAQVTLLFTWA-------------------NSVGGKSG 300
Query: 301 FTGHHFNSKIGAEDGAQGVLLHHKTANGRPSVTYAIAAEATDDVHVSLCPCFVISGESEG 360
FTGHHFNSK+ AEDGA GVLLHH+TA GRP+VTYAIAAEATDDVHVSLCPCFVISG+SEG
Sbjct: 301 FTGHHFNSKMRAEDGAHGVLLHHRTAGGRPTVTYAIAAEATDDVHVSLCPCFVISGDSEG 360
Query: 361 ISAKDMWQEIKNHGSFDKLGSVGAYEGSKPGSSIGAAIAATLTVPPRSARTVTFSLAWDC 420
ISAKDMWQEIKNHGSFDKLG+V A GSKPGSSIGAA+AATLT+PP SARTVTFSLAWDC
Sbjct: 361 ISAKDMWQEIKNHGSFDKLGNVAANGGSKPGSSIGAAVAATLTIPPASARTVTFSLAWDC 420
Query: 421 PEVKFDGKTYHR--------------------------------------YPVTLFNELY 480
PEVKFDGKTYHR Y VTLFNELY
Sbjct: 421 PEVKFDGKTYHRQYTKFYGTLGDAARTIAHDAILEAWQRPIIEDKRLPEWYAVTLFNELY 480
Query: 481 FLNSGGTIWTDGLAPLQNLSTISDKKYFLERSKSHPNGGAPTADHRKDVAVDILERMSQI 540
FLNSGGTIWTDGL PLQNLSTIS KKYFLERSKS PNGG P HRKDVAVDILERMSQI
Sbjct: 481 FLNSGGTIWTDGLPPLQNLSTISHKKYFLERSKSEPNGGVPNGVHRKDVAVDILERMSQI 540
Query: 541 FDQTHGGAGPSNAALGTRLLHPGEENVGNLLLVEGSQYLMWNTYDVHFYSSFALIMLLPK 600
FDQT GGAGPSNAALGTRLLHPGEENVGNLLLVEGSQYLMWNTYDVHFYSSFALIML PK
Sbjct: 541 FDQTDGGAGPSNAALGTRLLHPGEENVGNLLLVEGSQYLMWNTYDVHFYSSFALIMLFPK 600
Query: 601 LELSIQRDFAAAVLMHDPRKTKIMSDGNWVPRKVLGAVPHDIGFNDPWFEVNAYNLLNVT 660
LELSIQRDFAAAVLMHDPRK KIMSDGNWVPRKVLGAVPHDIGFNDPWFEVNAYNLLNVT
Sbjct: 601 LELSIQRDFAAAVLMHDPRKAKIMSDGNWVPRKVLGAVPHDIGFNDPWFEVNAYNLLNVT 660
Query: 661 RWKDLASKFVLQVYRDVVATGDKNFAQSVWPSVYVALAFMEQFDKDKDGMVENEGFPDQT 720
RWKDL SKFVLQVYRDVVATGDKNFA+SVWPSVYVALAFMEQFDKDKDGM+ENEGFPDQT
Sbjct: 661 RWKDLGSKFVLQVYRDVVATGDKNFAKSVWPSVYVALAFMEQFDKDKDGMIENEGFPDQT 720
Query: 721 YDTWTVKGVSAYCGGLWVAALQAASALASEVDDDAAAHYFWIKYQKARSVYDTLWNGSYF 780
YDTWTVKGVSAYCGGLWVAALQAASALASEVDD+AAAHYFWIKYQKARSVY+TLWNGSYF
Sbjct: 721 YDTWTVKGVSAYCGGLWVAALQAASALASEVDDEAAAHYFWIKYQKARSVYETLWNGSYF 780
Query: 781 NYDNSKGPWSSSIQADQLAGQWYARACGLCPIADEEKIRSALEKIYNFN---VKGGTRGA 840
NYDNSKGPWSSSIQADQLAGQWYARACGLCPIADEEKIR ALEKIYNFN VKGGTRGA
Sbjct: 781 NYDNSKGPWSSSIQADQLAGQWYARACGLCPIADEEKIRGALEKIYNFNVMKVKGGTRGA 840
Query: 841 VNGMFPDGRVDKSILQPKEIWAGVTYSVAASMIQEGLVETGFQTAMGIHQAAWAQDGLGY 900
VNGMFPDG VDKSILQPKEIWAGVTYSVAA+MIQEGLVETGFQTAMG++ AAWAQDGLGY
Sbjct: 841 VNGMFPDGSVDKSILQPKEIWAGVTYSVAATMIQEGLVETGFQTAMGVYHAAWAQDGLGY 900
Query: 901 SFQTPEAWDVDDRYRSIGYMRPMAIWAMQWALMS-KKPTKIPTKALSKMEETAFATQHAA 952
SFQTPEAWDVDDR+RSI YMRP+AIWAMQWA+MS KPTK PTKA S+MEE+AFATQH A
Sbjct: 901 SFQTPEAWDVDDRFRSIAYMRPLAIWAMQWAMMSDSKPTKFPTKAFSEMEESAFATQHTA 935
BLAST of Cla97C08G145420 vs. ExPASy TrEMBL
Match:
A0A1S3CB21 (Non-lysosomal glucosylceramidase OS=Cucumis melo OX=3656 GN=LOC103498676 PE=3 SV=1)
HSP 1 Score: 1651.3 bits (4275), Expect = 0.0e+00
Identity = 823/1003 (82.05%), Postives = 852/1003 (84.95%), Query Frame = 0
Query: 1 MEKGGNGASSTEVDPSKPPSLTWKRKLNFTGKSPESFSFTLTDAWHLGSLGYRLWRHGKE 60
MEKGGNGASSTEVDPSKPPSLTWKRKL+FTGKSPE FSFTLTDAWH+G LGYRLWR+GKE
Sbjct: 1 MEKGGNGASSTEVDPSKPPSLTWKRKLDFTGKSPECFSFTLTDAWHMGMLGYRLWRNGKE 60
Query: 61 EAAKGRVIMTASANFFQIDSVFLFHLIIVSLYSIFSLSLSLSLCPQIPLYEFFSDQPITC 120
E AKGR IP+YEFFSDQPITC
Sbjct: 61 EIAKGR----------------------------------------IPIYEFFSDQPITC 120
Query: 121 YHGVSLGGIGAGSIGRSYRGEFQRFQMFYGPCEDEPILANQFSVFVSRPNGKKFSSVLCS 180
YHGVSLGGIGAGSIGRSYRGEFQRFQMFYGPCEDEP+LANQFSVFVSRPNG KFSSVLCS
Sbjct: 121 YHGVSLGGIGAGSIGRSYRGEFQRFQMFYGPCEDEPVLANQFSVFVSRPNGNKFSSVLCS 180
Query: 181 TKPQKSKDGKQDGIESWDWNLSGENSTYHALFPRSWTVYDGEPDPDLKIVCRQISPFIPH 240
KPQK KDG Q GI SWDWNLSGENSTYHALFPRSWT+Y+GEPDPDLKIVCRQISP IPH
Sbjct: 181 AKPQKFKDGNQTGIGSWDWNLSGENSTYHALFPRSWTIYNGEPDPDLKIVCRQISPIIPH 240
Query: 241 NYKESSFPVSVFTFSLFNEGQTSAQVTLLFTWAVSAANYFLFNFLYFIFITVNSVGGKSG 300
NYKESSFPVSVFTF+L NEGQTSAQVTLLFTWA NSVGGKSG
Sbjct: 241 NYKESSFPVSVFTFNLSNEGQTSAQVTLLFTWA-------------------NSVGGKSG 300
Query: 301 FTGHHFNSKIGAEDGAQGVLLHHKTANGRPSVTYAIAAEATDDVHVSLCPCFVISGESEG 360
FTGHHFNSK+ AEDGA GVLLHH+TA GRP+VTYAIAAEATDDVHVSLCPCFVISG+SEG
Sbjct: 301 FTGHHFNSKMRAEDGAHGVLLHHRTAGGRPTVTYAIAAEATDDVHVSLCPCFVISGDSEG 360
Query: 361 ISAKDMWQEIKNHGSFDKLGSVGAYEGSKPGSSIGAAIAATLTVPPRSARTVTFSLAWDC 420
ISAKDMWQEIKNHGSFDKLG+V A GSKPGSSIGAA+AATLT+PP SARTVTFSLAWDC
Sbjct: 361 ISAKDMWQEIKNHGSFDKLGNVAANGGSKPGSSIGAAVAATLTIPPASARTVTFSLAWDC 420
Query: 421 PEVKFDGKTYHR-----------------------------------------------Y 480
PEVKFDGKTYHR Y
Sbjct: 421 PEVKFDGKTYHRRYSKFYGTLGDAARTIAHDAILEHGKWEAEIEAWQRPIIEDKRLPEWY 480
Query: 481 PVTLFNELYFLNSGGTIWTDGLAPLQNLSTISDKKYFLERSKSHPNGGAPTADHRKDVAV 540
VTLFNELYFLNSGGTIWTDGL PLQNLSTIS KKYFLERSKS PNGG P HRKDVAV
Sbjct: 481 TVTLFNELYFLNSGGTIWTDGLPPLQNLSTISHKKYFLERSKSEPNGGVPNGVHRKDVAV 540
Query: 541 DILERMSQIFDQTHGGAGPSNAALGTRLLHPGEENVGNLLLVEGSQYLMWNTYDVHFYSS 600
DILERMSQIFDQT GGAGPSNAALGTRLLHPGEENVGNLLLVEGSQYLMWNTYDVHFYSS
Sbjct: 541 DILERMSQIFDQTDGGAGPSNAALGTRLLHPGEENVGNLLLVEGSQYLMWNTYDVHFYSS 600
Query: 601 FALIMLLPKLELSIQRDFAAAVLMHDPRKTKIMSDGNWVPRKVLGAVPHDIGFNDPWFEV 660
FALIML PKLELSIQRDFAAAVLMHDPRK KIMSDGNWVPRKVLGAVPHDIGFNDPWFEV
Sbjct: 601 FALIMLFPKLELSIQRDFAAAVLMHDPRKAKIMSDGNWVPRKVLGAVPHDIGFNDPWFEV 660
Query: 661 NAYNLLNVTRWKDLASKFVLQVYRDVVATGDKNFAQSVWPSVYVALAFMEQFDKDKDGMV 720
NAYNLLNVTRWKDL SKFVLQVYRDVVATGDKNFA+SVWPSVYVALAFMEQFDKDKDGM+
Sbjct: 661 NAYNLLNVTRWKDLGSKFVLQVYRDVVATGDKNFAKSVWPSVYVALAFMEQFDKDKDGMI 720
Query: 721 ENEGFPDQTYDTWTVKGVSAYCGGLWVAALQAASALASEVDDDAAAHYFWIKYQKARSVY 780
ENEGFPDQTYDTWTVKGVSAYCGGLWVAALQAASALASEVDD+AAAHYFWIKYQKARSVY
Sbjct: 721 ENEGFPDQTYDTWTVKGVSAYCGGLWVAALQAASALASEVDDEAAAHYFWIKYQKARSVY 780
Query: 781 DTLWNGSYFNYDNSKGPWSSSIQADQLAGQWYARACGLCPIADEEKIRSALEKIYNFN-- 840
+TLWNGSYFNYDNSKGPWSSSIQADQLAGQWYARACGLCPIADEEKIR ALEKIYNFN
Sbjct: 781 ETLWNGSYFNYDNSKGPWSSSIQADQLAGQWYARACGLCPIADEEKIRGALEKIYNFNVM 840
Query: 841 -VKGGTRGAVNGMFPDGRVDKSILQPKEIWAGVTYSVAASMIQEGLVETGFQTAMGIHQA 900
VKGGTRGAVNGMFPDG VDKSILQPKEIWAGVTYSVAA+MIQEGLVETGFQTAMG++ A
Sbjct: 841 KVKGGTRGAVNGMFPDGSVDKSILQPKEIWAGVTYSVAATMIQEGLVETGFQTAMGVYHA 900
Query: 901 AWAQDGLGYSFQTPEAWDVDDRYRSIGYMRPMAIWAMQWALMS-KKPTKIPTKALSKMEE 952
AWAQDGLGYSFQTPEAWDVDDR+RSI YMRP+AIWAMQWA+MS KPTK PTKA S+MEE
Sbjct: 901 AWAQDGLGYSFQTPEAWDVDDRFRSIAYMRPLAIWAMQWAMMSDSKPTKFPTKAFSEMEE 944
BLAST of Cla97C08G145420 vs. ExPASy TrEMBL
Match:
A0A1S4E2H5 (Non-lysosomal glucosylceramidase OS=Cucumis melo OX=3656 GN=LOC103498676 PE=3 SV=1)
HSP 1 Score: 1638.2 bits (4241), Expect = 0.0e+00
Identity = 823/1026 (80.21%), Postives = 852/1026 (83.04%), Query Frame = 0
Query: 1 MEKGGNGASSTE-----------------------VDPSKPPSLTWKRKLNFTGKSPESF 60
MEKGGNGASSTE VDPSKPPSLTWKRKL+FTGKSPE F
Sbjct: 1 MEKGGNGASSTEVHHLLHPWLLNSFNRFGFQQALFVDPSKPPSLTWKRKLDFTGKSPECF 60
Query: 61 SFTLTDAWHLGSLGYRLWRHGKEEAAKGRVIMTASANFFQIDSVFLFHLIIVSLYSIFSL 120
SFTLTDAWH+G LGYRLWR+GKEE AKGR
Sbjct: 61 SFTLTDAWHMGMLGYRLWRNGKEEIAKGR------------------------------- 120
Query: 121 SLSLSLCPQIPLYEFFSDQPITCYHGVSLGGIGAGSIGRSYRGEFQRFQMFYGPCEDEPI 180
IP+YEFFSDQPITCYHGVSLGGIGAGSIGRSYRGEFQRFQMFYGPCEDEP+
Sbjct: 121 ---------IPIYEFFSDQPITCYHGVSLGGIGAGSIGRSYRGEFQRFQMFYGPCEDEPV 180
Query: 181 LANQFSVFVSRPNGKKFSSVLCSTKPQKSKDGKQDGIESWDWNLSGENSTYHALFPRSWT 240
LANQFSVFVSRPNG KFSSVLCS KPQK KDG Q GI SWDWNLSGENSTYHALFPRSWT
Sbjct: 181 LANQFSVFVSRPNGNKFSSVLCSAKPQKFKDGNQTGIGSWDWNLSGENSTYHALFPRSWT 240
Query: 241 VYDGEPDPDLKIVCRQISPFIPHNYKESSFPVSVFTFSLFNEGQTSAQVTLLFTWAVSAA 300
+Y+GEPDPDLKIVCRQISP IPHNYKESSFPVSVFTF+L NEGQTSAQVTLLFTWA
Sbjct: 241 IYNGEPDPDLKIVCRQISPIIPHNYKESSFPVSVFTFNLSNEGQTSAQVTLLFTWA---- 300
Query: 301 NYFLFNFLYFIFITVNSVGGKSGFTGHHFNSKIGAEDGAQGVLLHHKTANGRPSVTYAIA 360
NSVGGKSGFTGHHFNSK+ AEDGA GVLLHH+TA GRP+VTYAIA
Sbjct: 301 ---------------NSVGGKSGFTGHHFNSKMRAEDGAHGVLLHHRTAGGRPTVTYAIA 360
Query: 361 AEATDDVHVSLCPCFVISGESEGISAKDMWQEIKNHGSFDKLGSVGAYEGSKPGSSIGAA 420
AEATDDVHVSLCPCFVISG+SEGISAKDMWQEIKNHGSFDKLG+V A GSKPGSSIGAA
Sbjct: 361 AEATDDVHVSLCPCFVISGDSEGISAKDMWQEIKNHGSFDKLGNVAANGGSKPGSSIGAA 420
Query: 421 IAATLTVPPRSARTVTFSLAWDCPEVKFDGKTYHR------------------------- 480
+AATLT+PP SARTVTFSLAWDCPEVKFDGKTYHR
Sbjct: 421 VAATLTIPPASARTVTFSLAWDCPEVKFDGKTYHRRYSKFYGTLGDAARTIAHDAILEHG 480
Query: 481 ----------------------YPVTLFNELYFLNSGGTIWTDGLAPLQNLSTISDKKYF 540
Y VTLFNELYFLNSGGTIWTDGL PLQNLSTIS KKYF
Sbjct: 481 KWEAEIEAWQRPIIEDKRLPEWYTVTLFNELYFLNSGGTIWTDGLPPLQNLSTISHKKYF 540
Query: 541 LERSKSHPNGGAPTADHRKDVAVDILERMSQIFDQTHGGAGPSNAALGTRLLHPGEENVG 600
LERSKS PNGG P HRKDVAVDILERMSQIFDQT GGAGPSNAALGTRLLHPGEENVG
Sbjct: 541 LERSKSEPNGGVPNGVHRKDVAVDILERMSQIFDQTDGGAGPSNAALGTRLLHPGEENVG 600
Query: 601 NLLLVEGSQYLMWNTYDVHFYSSFALIMLLPKLELSIQRDFAAAVLMHDPRKTKIMSDGN 660
NLLLVEGSQYLMWNTYDVHFYSSFALIML PKLELSIQRDFAAAVLMHDPRK KIMSDGN
Sbjct: 601 NLLLVEGSQYLMWNTYDVHFYSSFALIMLFPKLELSIQRDFAAAVLMHDPRKAKIMSDGN 660
Query: 661 WVPRKVLGAVPHDIGFNDPWFEVNAYNLLNVTRWKDLASKFVLQVYRDVVATGDKNFAQS 720
WVPRKVLGAVPHDIGFNDPWFEVNAYNLLNVTRWKDL SKFVLQVYRDVVATGDKNFA+S
Sbjct: 661 WVPRKVLGAVPHDIGFNDPWFEVNAYNLLNVTRWKDLGSKFVLQVYRDVVATGDKNFAKS 720
Query: 721 VWPSVYVALAFMEQFDKDKDGMVENEGFPDQTYDTWTVKGVSAYCGGLWVAALQAASALA 780
VWPSVYVALAFMEQFDKDKDGM+ENEGFPDQTYDTWTVKGVSAYCGGLWVAALQAASALA
Sbjct: 721 VWPSVYVALAFMEQFDKDKDGMIENEGFPDQTYDTWTVKGVSAYCGGLWVAALQAASALA 780
Query: 781 SEVDDDAAAHYFWIKYQKARSVYDTLWNGSYFNYDNSKGPWSSSIQADQLAGQWYARACG 840
SEVDD+AAAHYFWIKYQKARSVY+TLWNGSYFNYDNSKGPWSSSIQADQLAGQWYARACG
Sbjct: 781 SEVDDEAAAHYFWIKYQKARSVYETLWNGSYFNYDNSKGPWSSSIQADQLAGQWYARACG 840
Query: 841 LCPIADEEKIRSALEKIYNFN---VKGGTRGAVNGMFPDGRVDKSILQPKEIWAGVTYSV 900
LCPIADEEKIR ALEKIYNFN VKGGTRGAVNGMFPDG VDKSILQPKEIWAGVTYSV
Sbjct: 841 LCPIADEEKIRGALEKIYNFNVMKVKGGTRGAVNGMFPDGSVDKSILQPKEIWAGVTYSV 900
Query: 901 AASMIQEGLVETGFQTAMGIHQAAWAQDGLGYSFQTPEAWDVDDRYRSIGYMRPMAIWAM 952
AA+MIQEGLVETGFQTAMG++ AAWAQDGLGYSFQTPEAWDVDDR+RSI YMRP+AIWAM
Sbjct: 901 AATMIQEGLVETGFQTAMGVYHAAWAQDGLGYSFQTPEAWDVDDRFRSIAYMRPLAIWAM 960
BLAST of Cla97C08G145420 vs. TAIR 10
Match:
AT5G49900.1 (Beta-glucosidase, GBA2 type family protein )
HSP 1 Score: 1098.6 bits (2840), Expect = 0.0e+00
Identity = 556/999 (55.66%), Postives = 693/999 (69.37%), Query Frame = 0
Query: 8 ASSTEVDPSKPPSLTWKRKLNFTGKSPESFSFTLTDAWHLGSLGYRLWRHGKEEAAKGRV 67
+S T+VDP+ P SLTW+RK++ K+P F+ ++ + + L +G RLW +EEAAKGR
Sbjct: 18 SSDTKVDPAVPASLTWQRKIDSDVKAPREFNLSVKEIFQLAPVGIRLWFLCREEAAKGR- 77
Query: 68 IMTASANFFQIDSVFLFHLIIVSLYSIFSLSLSLSLCPQIPLYEFFSDQPITCYHGVSLG 127
+ + FS +T HGV LG
Sbjct: 78 ---------------------------------------LAFIDPFSKHSVTSSHGVPLG 137
Query: 128 GIGAGSIGRSYRGEFQRFQMFYGPCEDEPILANQFSVFVSRPNGKKFSSVLCSTKPQKSK 187
GIGAGSIGRS++GEFQR+Q+F CEDEP+LANQFS FVSR NGKK+SSVLC P+ K
Sbjct: 138 GIGAGSIGRSFKGEFQRWQLFPPKCEDEPVLANQFSAFVSRANGKKYSSVLCPRNPKLDK 197
Query: 188 DGKQDGIESWDWNLSGENSTYHALFPRSWTVYDGEPDPDLKIVCRQISPFIPHNYKESSF 247
+ GI SWDWNL G+ STYHAL+PRSWT+Y+GEPDP+L+IVCRQ+SPFIPHNYKESSF
Sbjct: 198 QDSESGIGSWDWNLKGDKSTYHALYPRSWTMYEGEPDPELRIVCRQVSPFIPHNYKESSF 257
Query: 248 PVSVFTFSLFNEGQTSAQVTLLFTWAVSAANYFLFNFLYFIFITVNSVGGKSGFTGHHFN 307
PVSVFTF+L N G T+A VTLLFTWA NSVGG S F+G H+N
Sbjct: 258 PVSVFTFTLHNLGNTTADVTLLFTWA-------------------NSVGGDSEFSGGHYN 317
Query: 308 SKIGAEDGAQGVLLHHKTANGRPSVTYAIAAEATDDVHVSLCPCFVISGESEGISAKDMW 367
SKI DG QGVLLHHKTANG PS++YAI+A+ATD V VS CP F++SG+ +GI+AKDMW
Sbjct: 318 SKITMNDGVQGVLLHHKTANGLPSLSYAISAQATDGVSVSACPFFIVSGKQDGITAKDMW 377
Query: 368 QEIKNHGSFDKLGSVGAYEGSKPGSSIGAAIAATLTVPPRSARTVTFSLAWDCPEVKF-D 427
Q +K +GSFD L + A S GSSIGAA+AA++TV P +R VTFSLAWDCPEV+F
Sbjct: 378 QAVKENGSFDHLKASEASMQSDHGSSIGAAVAASVTVLPGESRIVTFSLAWDCPEVQFPS 437
Query: 428 GKTYHR-----------------------------------------------YPVTLFN 487
GK Y R YPVTLFN
Sbjct: 438 GKIYSRRYTKFYGNNGDAAAQIAHDAILGHSQWESWIEDWQRPILEDKRLPAWYPVTLFN 497
Query: 488 ELYFLNSGGTIWTDGLAPLQNLSTISDKKYFLERSKSHPNGGAPTADHRKDVAVDILERM 547
ELY+LNSGGT+WTDG +P+ +L+ + +KK+ L++S+ H+ D AV +LE+M
Sbjct: 498 ELYYLNSGGTLWTDGSSPVHSLAGVREKKFSLDKSQLGLKNDI-DVPHQNDTAVSVLEKM 557
Query: 548 SQIFDQTHGGAGPSNAALGTRLLHPGEENVGNLLLVEGSQYLMWNTYDVHFYSSFALIML 607
+ ++ H SN+A GT+LL GEEN+G+ L +EG +Y MWNTYDVHFY+SFAL+ML
Sbjct: 558 ASTLEELHAST-TSNSAFGTKLLEEGEENIGHFLYLEGIEYRMWNTYDVHFYASFALVML 617
Query: 608 LPKLELSIQRDFAAAVLMHDPRKTKIMSDGNWVPRKVLGAVPHDIGFNDPWFEVNAYNLL 667
PKLELSIQRDFAAAV++HDP K K +S+G WV RKVLGAVPHD+G NDPWFEVN Y L
Sbjct: 618 FPKLELSIQRDFAAAVMLHDPTKVKTLSEGQWVQRKVLGAVPHDLGINDPWFEVNGYTLH 677
Query: 668 NVTRWKDLASKFVLQVYRDVVATGDKNFAQSVWPSVYVALAFMEQFDKDKDGMVENEGFP 727
N RWKDL KFVLQVYRDVVATGDK FA +VWPSVYVA+A+M QFDKD DGM+ENEGFP
Sbjct: 678 NTDRWKDLNPKFVLQVYRDVVATGDKKFASAVWPSVYVAMAYMAQFDKDGDGMIENEGFP 737
Query: 728 DQTYDTWTVKGVSAYCGGLWVAALQAASALASEVDDDAAAHYFWIKYQKARSVYD-TLWN 787
DQTYDTW+ GVSAYCGGLWVAALQAASALA V D + YFW K+QKA+ VY+ LWN
Sbjct: 738 DQTYDTWSASGVSAYCGGLWVAALQAASALARVVGDKNSQDYFWSKFQKAKVVYEKKLWN 797
Query: 788 GSYFNYDNSKGPWSSSIQADQLAGQWYARACGLCPIADEEKIRSALEKIYNFNV---KGG 847
GSYFNYDNS +SS+IQADQLAGQWYARA GL PI DE+K R+ALEK+YN+NV K G
Sbjct: 798 GSYFNYDNSGSQYSSTIQADQLAGQWYARASGLLPIVDEDKARTALEKVYNYNVMKIKDG 857
Query: 848 TRGAVNGMFPDGRVDKSILQPKEIWAGVTYSVAASMIQEGLVETGFQTAMGIHQAAWAQD 907
RGAVNGM P+G+VD + +Q +EIW+GVTY+++A+MIQEGLVE FQTA GI++AAW++
Sbjct: 858 KRGAVNGMHPNGKVDTASMQSREIWSGVTYALSATMIQEGLVEMAFQTASGIYEAAWSET 917
Query: 908 GLGYSFQTPEAWDVDDRYRSIGYMRPMAIWAMQWAL--MSKKPTKI---PTKALSKMEET 950
GLGYSFQTPE+W+ D YRS+ YMRP+AIWAMQWAL S+K ++ P + ++E +
Sbjct: 918 GLGYSFQTPESWNTVDEYRSLTYMRPLAIWAMQWALTKTSQKQEQLGLEPEQQEPELEPS 954
BLAST of Cla97C08G145420 vs. TAIR 10
Match:
AT1G33700.1 (Beta-glucosidase, GBA2 type family protein )
HSP 1 Score: 1057.7 bits (2734), Expect = 5.3e-309
Identity = 557/995 (55.98%), Postives = 657/995 (66.03%), Query Frame = 0
Query: 17 KPPSLTWKRKLNFTGKSPESFSFTLTDAWHLGSLGYRLWRHGKEEAAKGRVIMTASANFF 76
K P +W+RKLN K+P F + D HL LGYRLWRH K+EAAKGR
Sbjct: 16 KLPPFSWERKLNSQAKTPSEFKLSKRDHMHLFPLGYRLWRHTKDEAAKGRA--------- 75
Query: 77 QIDSVFLFHLIIVSLYSIFSLSLSLSLCPQIPLYEFFSDQPITCYHGVSLGGIGAGSIGR 136
+++ F IT HGV LGGIG+GSIGR
Sbjct: 76 -------------------------------SIFDIFRKHHITGDHGVPLGGIGSGSIGR 135
Query: 137 SYRGEFQRFQMFYGPCEDEPILANQFSVFVSRPNGKKFSSVLCSTKPQKSKD-------- 196
SY+GEFQ+F++F CE+ PIL NQFS FVSRP G K S+VLC +KPQ KD
Sbjct: 136 SYKGEFQQFKLFPKICEEAPILTNQFSAFVSRPGGVKHSTVLCPSKPQVVKDNGGYLCQG 195
Query: 197 -GKQDGIESWDWNLSGENSTYHALFPRSWTVYDGEPDPDLKIVCRQISPFIPHNYKESSF 256
GIESWDWN++GE STYHAL+PRSWTVYDGEPDP+L+IV RQ+SPFIPHNY+ESS
Sbjct: 196 QAPNMGIESWDWNMTGEKSTYHALYPRSWTVYDGEPDPELRIVSRQVSPFIPHNYEESSL 255
Query: 257 PVSVFTFSLFNEGQTSAQVTLLFTWAVSAANYFLFNFLYFIFITVNSVGGKSGFTGHHFN 316
PVSVF F++ N G A VTLLFTW NSVGG SG TG HFN
Sbjct: 256 PVSVFNFTVTNTGAEPAIVTLLFTWE-------------------NSVGGASGLTGQHFN 315
Query: 317 SKIGAEDGAQGVLLHHKTANGRPSVTYAIAAEATDDVHVSLCPCFVISGESEG-ISAKDM 376
S + A+DG V L HKTANG P V+YAIAA+ T+DV VS CPCF++SG + I+A DM
Sbjct: 316 STMKAKDGVHAVALQHKTANGHPPVSYAIAAKETEDVRVSSCPCFIVSGTTPNQITAGDM 375
Query: 377 WQEIKNHGSFDKLGSVGAYEGSKPGSSIGAAIAATLTVPPRSARTVTFSLAWDCPEVKFD 436
W EIK + SFDKL S A SKPG+SIGAAIAA + VPP RTVTFSL+WDCPE +FD
Sbjct: 376 WDEIKKNASFDKLTS-NACSPSKPGTSIGAAIAAKVKVPPGCDRTVTFSLSWDCPEARFD 435
Query: 437 GKTYHR-----------------------------------------------YPVTLFN 496
KTYHR Y VTLFN
Sbjct: 436 EKTYHRRYTRFYGSLGNAAVAMAHDALLNFPEWETQIEEWQAPVLADTTLPEWYRVTLFN 495
Query: 497 ELYFLNSGGTIWTDGLAPLQNLSTISDKKYFLERSKSHPNGGAPTADHRKDVAVDILERM 556
ELY+ NSGGT+WTDGL P Q+L +I +K L S N + +VA+DIL R+
Sbjct: 496 ELYYFNSGGTMWTDGLPPKQSLDSIGRRKISLGLSTIDKND-----QDQNNVALDILGRI 555
Query: 557 SQIFDQTHGGAGPSNAALGTRLLHPGEENVGNLLLVEGSQYLMWNTYDVHFYSSFALIML 616
+ Q H SNAALG ++ EN+G L +EG QYLM+NTYDVHFYSSFAL+ML
Sbjct: 556 DAVCSQIHAPL-TSNAALGNTMIQNTTENIGQFLYLEGVQYLMYNTYDVHFYSSFALLML 615
Query: 617 LPKLELSIQRDFAAAVLMHDPRKTKIMSDGNWVPRKVLGAVPHDIGFNDPWFEVNAYNLL 676
PK+ELSIQRDFAAAVLMHD K ++MS G +V RKVLGAVPHDIG NDPWFE+NAYNL
Sbjct: 616 FPKIELSIQRDFAAAVLMHDSSKKQVMSSGEFVTRKVLGAVPHDIGLNDPWFELNAYNLF 675
Query: 677 NVTRWKDLASKFVLQVYRDVVATGDKNFAQSVWPSVYVALAFMEQFDKDKDGMVENEGFP 736
N RWKDL SKFVLQVYRDVVATGD NFA++VWPSVY A+A+++QFDKD DGM+ENEGFP
Sbjct: 676 NTDRWKDLNSKFVLQVYRDVVATGDLNFAKAVWPSVYTAIAYLDQFDKDGDGMIENEGFP 735
Query: 737 DQTYDTWTVKGVSAYCGGLWVAALQAASALASEVDDDAAAHYFWIKYQKARSVYDTLWNG 796
DQTYD W+ GVSAYCGGLWVAALQA SALA E+ D+ AA YF KY+KARSVY+ LWNG
Sbjct: 736 DQTYDAWSCSGVSAYCGGLWVAALQAGSALAREIGDNGAAVYFNAKYEKARSVYEKLWNG 795
Query: 797 SYFNYDNSKGPWSSSIQADQLAGQWYARACGLCPIADEEKIRSALEKIYNFN---VKGGT 856
SYFNYDNS+ SSSI ADQ+AGQWYARACGL PIA EE I+ ALE +Y+FN V+ GT
Sbjct: 796 SYFNYDNSRSGSSSSILADQMAGQWYARACGLKPIAKEEWIKKALETVYDFNVMRVRDGT 855
Query: 857 RGAVNGMFPDGRVDKSILQPKEIWAGVTYSVAASMIQEGLVETGFQTAMGIHQAAWAQDG 916
RGAVNGM PDGRVD S + +E+WAG TYSVAA MIQEGL + GF+TA GI++AAW+ G
Sbjct: 856 RGAVNGMLPDGRVDTSTMVSREVWAGTTYSVAACMIQEGLADKGFRTASGIYEAAWSDRG 915
Query: 917 LGYSFQTPEAWDVDDRYRSIGYMRPMAIWAMQWALMSKKPTKIPTKALSKMEETAFAT-- 949
LG +FQTPEAW +D YRS+ YMRP+AIW +QWA KP + +L EE A +
Sbjct: 916 LGCAFQTPEAWTTNDEYRSLCYMRPLAIWGIQWAHTMPKPNREQEVSLRPQEEDATSVLF 943
BLAST of Cla97C08G145420 vs. TAIR 10
Match:
AT1G33700.2 (Beta-glucosidase, GBA2 type family protein )
HSP 1 Score: 1057.7 bits (2734), Expect = 5.3e-309
Identity = 557/995 (55.98%), Postives = 657/995 (66.03%), Query Frame = 0
Query: 17 KPPSLTWKRKLNFTGKSPESFSFTLTDAWHLGSLGYRLWRHGKEEAAKGRVIMTASANFF 76
K P +W+RKLN K+P F + D HL LGYRLWRH K+EAAKGR
Sbjct: 16 KLPPFSWERKLNSQAKTPSEFKLSKRDHMHLFPLGYRLWRHTKDEAAKGRA--------- 75
Query: 77 QIDSVFLFHLIIVSLYSIFSLSLSLSLCPQIPLYEFFSDQPITCYHGVSLGGIGAGSIGR 136
+++ F IT HGV LGGIG+GSIGR
Sbjct: 76 -------------------------------SIFDIFRKHHITGDHGVPLGGIGSGSIGR 135
Query: 137 SYRGEFQRFQMFYGPCEDEPILANQFSVFVSRPNGKKFSSVLCSTKPQKSKD-------- 196
SY+GEFQ+F++F CE+ PIL NQFS FVSRP G K S+VLC +KPQ KD
Sbjct: 136 SYKGEFQQFKLFPKICEEAPILTNQFSAFVSRPGGVKHSTVLCPSKPQVVKDNGGYLCQG 195
Query: 197 -GKQDGIESWDWNLSGENSTYHALFPRSWTVYDGEPDPDLKIVCRQISPFIPHNYKESSF 256
GIESWDWN++GE STYHAL+PRSWTVYDGEPDP+L+IV RQ+SPFIPHNY+ESS
Sbjct: 196 QAPNMGIESWDWNMTGEKSTYHALYPRSWTVYDGEPDPELRIVSRQVSPFIPHNYEESSL 255
Query: 257 PVSVFTFSLFNEGQTSAQVTLLFTWAVSAANYFLFNFLYFIFITVNSVGGKSGFTGHHFN 316
PVSVF F++ N G A VTLLFTW NSVGG SG TG HFN
Sbjct: 256 PVSVFNFTVTNTGAEPAIVTLLFTWE-------------------NSVGGASGLTGQHFN 315
Query: 317 SKIGAEDGAQGVLLHHKTANGRPSVTYAIAAEATDDVHVSLCPCFVISGESEG-ISAKDM 376
S + A+DG V L HKTANG P V+YAIAA+ T+DV VS CPCF++SG + I+A DM
Sbjct: 316 STMKAKDGVHAVALQHKTANGHPPVSYAIAAKETEDVRVSSCPCFIVSGTTPNQITAGDM 375
Query: 377 WQEIKNHGSFDKLGSVGAYEGSKPGSSIGAAIAATLTVPPRSARTVTFSLAWDCPEVKFD 436
W EIK + SFDKL S A SKPG+SIGAAIAA + VPP RTVTFSL+WDCPE +FD
Sbjct: 376 WDEIKKNASFDKLTS-NACSPSKPGTSIGAAIAAKVKVPPGCDRTVTFSLSWDCPEARFD 435
Query: 437 GKTYHR-----------------------------------------------YPVTLFN 496
KTYHR Y VTLFN
Sbjct: 436 EKTYHRRYTRFYGSLGNAAVAMAHDALLNFPEWETQIEEWQAPVLADTTLPEWYRVTLFN 495
Query: 497 ELYFLNSGGTIWTDGLAPLQNLSTISDKKYFLERSKSHPNGGAPTADHRKDVAVDILERM 556
ELY+ NSGGT+WTDGL P Q+L +I +K L S N + +VA+DIL R+
Sbjct: 496 ELYYFNSGGTMWTDGLPPKQSLDSIGRRKISLGLSTIDKND-----QDQNNVALDILGRI 555
Query: 557 SQIFDQTHGGAGPSNAALGTRLLHPGEENVGNLLLVEGSQYLMWNTYDVHFYSSFALIML 616
+ Q H SNAALG ++ EN+G L +EG QYLM+NTYDVHFYSSFAL+ML
Sbjct: 556 DAVCSQIHAPL-TSNAALGNTMIQNTTENIGQFLYLEGVQYLMYNTYDVHFYSSFALLML 615
Query: 617 LPKLELSIQRDFAAAVLMHDPRKTKIMSDGNWVPRKVLGAVPHDIGFNDPWFEVNAYNLL 676
PK+ELSIQRDFAAAVLMHD K ++MS G +V RKVLGAVPHDIG NDPWFE+NAYNL
Sbjct: 616 FPKIELSIQRDFAAAVLMHDSSKKQVMSSGEFVTRKVLGAVPHDIGLNDPWFELNAYNLF 675
Query: 677 NVTRWKDLASKFVLQVYRDVVATGDKNFAQSVWPSVYVALAFMEQFDKDKDGMVENEGFP 736
N RWKDL SKFVLQVYRDVVATGD NFA++VWPSVY A+A+++QFDKD DGM+ENEGFP
Sbjct: 676 NTDRWKDLNSKFVLQVYRDVVATGDLNFAKAVWPSVYTAIAYLDQFDKDGDGMIENEGFP 735
Query: 737 DQTYDTWTVKGVSAYCGGLWVAALQAASALASEVDDDAAAHYFWIKYQKARSVYDTLWNG 796
DQTYD W+ GVSAYCGGLWVAALQA SALA E+ D+ AA YF KY+KARSVY+ LWNG
Sbjct: 736 DQTYDAWSCSGVSAYCGGLWVAALQAGSALAREIGDNGAAVYFNAKYEKARSVYEKLWNG 795
Query: 797 SYFNYDNSKGPWSSSIQADQLAGQWYARACGLCPIADEEKIRSALEKIYNFN---VKGGT 856
SYFNYDNS+ SSSI ADQ+AGQWYARACGL PIA EE I+ ALE +Y+FN V+ GT
Sbjct: 796 SYFNYDNSRSGSSSSILADQMAGQWYARACGLKPIAKEEWIKKALETVYDFNVMRVRDGT 855
Query: 857 RGAVNGMFPDGRVDKSILQPKEIWAGVTYSVAASMIQEGLVETGFQTAMGIHQAAWAQDG 916
RGAVNGM PDGRVD S + +E+WAG TYSVAA MIQEGL + GF+TA GI++AAW+ G
Sbjct: 856 RGAVNGMLPDGRVDTSTMVSREVWAGTTYSVAACMIQEGLADKGFRTASGIYEAAWSDRG 915
Query: 917 LGYSFQTPEAWDVDDRYRSIGYMRPMAIWAMQWALMSKKPTKIPTKALSKMEETAFAT-- 949
LG +FQTPEAW +D YRS+ YMRP+AIW +QWA KP + +L EE A +
Sbjct: 916 LGCAFQTPEAWTTNDEYRSLCYMRPLAIWGIQWAHTMPKPNREQEVSLRPQEEDATSVLF 943
BLAST of Cla97C08G145420 vs. TAIR 10
Match:
AT4G10060.1 (Beta-glucosidase, GBA2 type family protein )
HSP 1 Score: 1001.5 bits (2588), Expect = 4.5e-292
Identity = 532/1007 (52.83%), Postives = 646/1007 (64.15%), Query Frame = 0
Query: 1 MEKGGNGASSTE---VDPSKPPSLTWKRKLNFTGKSPESFSFTLTDAWHLGSLGYRLWRH 60
MEK G+ S + V + P +TW+RKLN K+P F ++ D HL LGYRLWR+
Sbjct: 1 MEKNGHTESELQTQMVGNERLPQVTWQRKLNSKVKNPSEFKMSIRDVLHLFPLGYRLWRY 60
Query: 61 GKEEAAKGRVIMTASANFFQIDSVFLFHLIIVSLYSIFSLSLSLSLCPQIPLYEFFSDQP 120
KEEA KGR M Y+ F +
Sbjct: 61 TKEEAKKGRFSM----------------------------------------YDIFKKRH 120
Query: 121 ITCYHGVSLGGIGAGSIGRSYRGEFQRFQMFYGPCEDEPILANQFSVFVSRPNGKKFSSV 180
+ HGV LGGIG GSIGRSY+GEFQ+F++F CE+ PIL NQFSVFVSRP G +S+V
Sbjct: 121 VRGDHGVPLGGIGGGSIGRSYKGEFQQFKLFPKICEEAPILTNQFSVFVSRPGGLSYSTV 180
Query: 181 LCSTKPQKSKDGKQD-GIESWDWNLSGENSTYHALFPRSWTVYDGEPDPDLKIVCRQISP 240
LC TKP+ K +D GIESWDWN+ G+ STYHAL+PRSWTVY+ EPDP+L+IV RQ+SP
Sbjct: 181 LCPTKPKSVKGKTEDLGIESWDWNMEGDKSTYHALYPRSWTVYN-EPDPELRIVSRQVSP 240
Query: 241 FIPHNYKESSFPVSVFTFSLFNEGQTSAQVTLLFTWAVSAANYFLFNFLYFIFITVNSVG 300
FIPHNYKESS PVSVF F++ N G+ A VTLLFTW NSVG
Sbjct: 241 FIPHNYKESSLPVSVFEFTMSNLGKEEATVTLLFTWE-------------------NSVG 300
Query: 301 GKSGFTGHHFNSKIGAEDGAQGVLLHHKTANGRPSVTYAIAAEATDDVHVSLCPCFVISG 360
G SG TG HFNS I DG ++LHHKT NG P VTYAIAA+ T+DVHVS CPCF++SG
Sbjct: 301 GASGLTGEHFNSTIMERDGVHAIVLHHKTGNGHPPVTYAIAAQETEDVHVSECPCFLVSG 360
Query: 361 ES-EGISAKDMWQEIKNHGSFDKLGSVGAYEGSKPGSSIGAAIAATLTVPPRSARTVTFS 420
S + I+AK+MW EIK + SFD+L S S+PG+SIGAAIAA + VPP RTVTFS
Sbjct: 361 HSPKEITAKEMWDEIKKNKSFDELNSEPG-SPSRPGTSIGAAIAAKVKVPPGCDRTVTFS 420
Query: 421 LAWDCPEVKFDGKTYHR------------------------------------------- 480
L+WDCPEV+F+ KTYHR
Sbjct: 421 LSWDCPEVRFNEKTYHRRYTKFYGNLGDAAVKMARDALLNYVDWESQIEAWQSPILSDTT 480
Query: 481 ----YPVTLFNELYFLNSGGTIWTDGLAPLQNLSTISDKKYFLERSKSHPNGGAPTADHR 540
Y VTLFNELY+ NSGGTIWTDGL P K +ERSK T +
Sbjct: 481 LPDWYRVTLFNELYYFNSGGTIWTDGLPP----------KESIERSK-------VTNTEQ 540
Query: 541 KDVAVDILERMSQIFDQTHGGAGPSNAALGTRLLHPGEENVGNLLLVEGSQYLMWNTYDV 600
D+ +D+ ++++ + +Q + ++ EEN+G + +EG +YLM+NTYDV
Sbjct: 541 NDIVIDLFQKINAVCEQIYSPQSSNS-----------EENIGQFIYLEGIEYLMYNTYDV 600
Query: 601 HFYSSFALIMLLPKLELSIQRDFAAAVLMHDPRKTKIMSDGNWVPRKVLGAVPHDIGFND 660
HFYSSFAL+ L PKL LSIQRDFAA VL+ DP K KIMS G WV RK+LG+VPHDIG ND
Sbjct: 601 HFYSSFALLSLFPKLALSIQRDFAATVLIQDPTKKKIMSSGEWVTRKLLGSVPHDIGLND 660
Query: 661 PWFEVNAYNLLNVTRWKDLASKFVLQVYRDVVATGDKNFAQSVWPSVYVALAFMEQFDKD 720
PW E+N YN N RWKDL +KFVLQVYRDVVAT D++FA++VWPSVY A+A+++QFDKD
Sbjct: 661 PWLELNEYNFFNTDRWKDLNAKFVLQVYRDVVATNDQSFAKAVWPSVYTAVAYLDQFDKD 720
Query: 721 KDGMVENEGFPDQTYDTWTVKGVSAYCGGLWVAALQAASALASEVDDDAAAHYFWIKYQK 780
+DGM+ENEGFPDQTYD W+V GVSAYCGGLWVAALQAASA AS V ++A A YF KY+K
Sbjct: 721 EDGMIENEGFPDQTYDAWSVTGVSAYCGGLWVAALQAASAFASIVGENAVAIYFNAKYEK 780
Query: 781 ARSVYDTLWNGSYFNYDNSKGPWSSSIQADQLAGQWYARACGLCPIADEEKIRSALEKIY 840
A+ VY+ LWNGSYFNYD+S SSSI ADQLAGQWYARACGL PI EE I+ ALE IY
Sbjct: 781 AKIVYEKLWNGSYFNYDDSGSGSSSSILADQLAGQWYARACGLKPITKEEWIKKALETIY 840
Query: 841 NFN---VKGGTRGAVNGMFPDGRVDKSILQPKEIWAGVTYSVAASMIQEGLVETGFQTAM 900
FN VKGGTRGAVNGM +G+VD + L KE+WAG TYSVAA MIQEG E GFQTA
Sbjct: 841 EFNVMKVKGGTRGAVNGMSTEGKVDTNSLVSKEVWAGTTYSVAACMIQEGQREKGFQTAS 900
Query: 901 GIHQAAWAQDGLGYSFQTPEAWDVDDRYRSIGYMRPMAIWAMQWALMSKKPTKIPTKAL- 949
GI++A W+ GL SFQTPEAW+++D YRS+ YMRP+AIWA+QWAL + + L
Sbjct: 901 GIYEAIWSDRGLSCSFQTPEAWNMNDEYRSLCYMRPLAIWAIQWALTRTQSFGEEKQKLV 918
BLAST of Cla97C08G145420 vs. TAIR 10
Match:
AT3G24180.1 (Beta-glucosidase, GBA2 type family protein )
HSP 1 Score: 775.8 bits (2002), Expect = 4.0e-224
Identity = 427/981 (43.53%), Postives = 573/981 (58.41%), Query Frame = 0
Query: 12 EVDPSKPPSLTWKRKLNFTGKSPESFSFTLTDAWHLGSLGYRLWRHGKEEAAKGRVIMTA 71
+ D + PP W+R+LN + F+ T +A + LG RLW + +EEA+ GR
Sbjct: 29 DFDSAAPPPHAWRRRLNCHANILKEFTITFREAIKMVRLGIRLWSYVREEASHGR----- 88
Query: 72 SANFFQIDSVFLFHLIIVSLYSIFSLSLSLSLCPQIPLYEFFSDQ-PITCYHGVSLGGIG 131
+ P+ F + + GV LGG+G
Sbjct: 89 ----------------------------------KAPIDPFTKENCKPSASQGVPLGGMG 148
Query: 132 AGSIGRSYRGEFQRFQMFYGPCEDEPILANQFSVFVSRPNG-KKFSSVLCSTKPQKSKDG 191
+GSI R +RGEF+++Q+ G C+ P+++NQFS+F+SR G KK++SVL +
Sbjct: 149 SGSISRGFRGEFKQWQITPGTCDPSPMMSNQFSIFISRDGGHKKYASVLSPGQHGSLGKS 208
Query: 192 KQDGIESWDWNLSGENSTYHALFPRSWTVYDGEPDPDLKIVCRQISPFIPHNYKESSFPV 251
+ G+ SW WNL+G++STYHALFPR+WT+YDGEPDP+LKI CRQISPFIP+NY++SS P
Sbjct: 209 RDKGLSSWGWNLNGQHSTYHALFPRAWTIYDGEPDPELKISCRQISPFIPNNYRDSSLPA 268
Query: 252 SVFTFSLFNEGQTSAQVTLLFTWAVSAANYFLFNFLYFIFITVNSVGGKSGFTGHHFNSK 311
+VF ++L N G+ A+V+LLFTWA NS+GG S +G H N
Sbjct: 269 AVFVYTLVNTGKERAKVSLLFTWA-------------------NSMGGTSHMSGGHVNEP 328
Query: 312 IGAEDGAQGVLLHHKTANGRPSVTYAIAAEATDDVHVSLCPCFVISGESEGISAKDMWQE 371
EDG GVLLHHKT G P VT+AIAA T +V+V++ PCF +S E +AKDMW
Sbjct: 329 FIGEDGVSGVLLHHKTGKGNPPVTFAIAASETQNVNVTVLPCFGLS-EDSSFTAKDMWDT 388
Query: 372 IKNHGSFDKLG-SVGAYEGSKPGSSIGAAIAATLTVPPRSARTVTFSLAWDCPEVKFD-G 431
++ G FD+ + G S G +I AA++A+ V TV+F+L+W P+VKF G
Sbjct: 389 MEQDGKFDQENFNSGPSTPSLAGDTICAAVSASAWVEAHGKCTVSFALSWSSPKVKFSKG 448
Query: 432 KTYHR-----------------------------------------------YPVTLFNE 491
TY R Y TLFNE
Sbjct: 449 STYDRRYTKFYGTSPRAALDLVHDALTNYKRWEEDIEAWQNPILRDERLPEWYKFTLFNE 508
Query: 492 LYFLNSGGTIWTDGLAPLQNLSTISDKKYFLERSKSHPNGGAPTADHRKD------VAVD 551
LYFL +GGT+W D + N + S + + GG D R D V V
Sbjct: 509 LYFLVAGGTVWIDSSS--LNANGNSQHQQSGLGNSDGKVGGLDINDQRNDLGNGNSVGVK 568
Query: 552 ILERMSQIFDQTHGGAGPSNAALGTRLLHPGEENVGNLLLVEGSQYLMWNTYDVHFYSSF 611
+ +S I ++ + + T + G++ VG L +EG +Y+MW TYDVHFY+S+
Sbjct: 569 SNDEVSAIHNR-------NGLFVDTPHVDDGDD-VGRFLYLEGVEYVMWCTYDVHFYASY 628
Query: 612 ALIMLLPKLELSIQRDFAAAVLMHDPRKTKIMSDGNWVPRKVLGAVPHDIGFNDPWFEVN 671
AL+ML PK+EL+IQRDFA AVL D RK K +++GN RKV GAVPHD+G +DPW E+N
Sbjct: 629 ALLMLFPKIELNIQRDFAKAVLSEDGRKVKFLAEGNVGIRKVRGAVPHDLGMHDPWNEMN 688
Query: 672 AYNLLNVTRWKDLASKFVLQVYRDVVATGDKNFAQSVWPSVYVALAFMEQFDKDKDGMVE 731
AYN+ + +RWKDL KFVLQVYRD ATGD F VWP+V A+ +MEQFD+D D ++E
Sbjct: 689 AYNIHDTSRWKDLNPKFVLQVYRDFAATGDYQFGIDVWPAVRAAMEYMEQFDRDNDDLIE 748
Query: 732 NEGFPDQTYDTWTVKGVSAYCGGLWVAALQAASALASEVDDDAAAHYFWIKYQKARSVYD 791
N+GFPDQTYDTWTV GVSAYCG LW+AALQAA+A+A ++ D A K+ A++ +
Sbjct: 749 NDGFPDQTYDTWTVHGVSAYCGCLWLAALQAAAAMALQIGDKFFAELCKNKFLNAKAALE 808
Query: 792 T-LWNGSYFNYDNSKGPWSSSIQADQLAGQWYARACGLCPIADEEKIRSALEKIYNFNV- 851
T LWNGSYFNYD+ S SIQ DQLAGQWYA + GL P+ +E KIRS ++KI++FNV
Sbjct: 809 TKLWNGSYFNYDSGSSSNSKSIQTDQLAGQWYAASSGLPPLFEESKIRSTMQKIFDFNVM 868
Query: 852 --KGGTRGAVNGMFPDGRVDKSILQPKEIWAGVTYSVAASMIQEGLVETGFQTAMGIHQA 911
KGG GAVNGM PDG+VD + +Q +EIW GVTY+ AA+MI G+ E GF TA GI A
Sbjct: 869 KTKGGKMGAVNGMHPDGKVDDTCMQSREIWTGVTYAAAATMILSGMEEQGFTTAEGIFTA 928
Query: 912 AWAQDGLGYSFQTPEAWDVDDRYRSIGYMRPMAIWAMQWALMSKKPTKIPTKALSKMEET 932
W+++G GY FQTPE W +D YRS+ YMRP+AIW MQWAL K + ++ M+
Sbjct: 929 GWSEEGFGYWFQTPEGWTMDGHYRSLIYMRPLAIWGMQWALSLPKAI-LDAPQINMMDRV 939
The following BLAST results are available for this feature:
| Match Name | E-value | Identity | Description | |
|---|
| XP_038890278.1 | 0.0e+00 | 83.22 | non-lysosomal glucosylceramidase-like isoform X1 [Benincasa hispida] | [more] |
| XP_004141546.1 | 0.0e+00 | 82.55 | non-lysosomal glucosylceramidase isoform X1 [Cucumis sativus] >KGN52666.1 hypoth... | [more] |
| KAA0039284.1 | 0.0e+00 | 82.80 | non-lysosomal glucosylceramidase isoform X2 [Cucumis melo var. makuwa] | [more] |
| TYK00469.1 | 0.0e+00 | 82.80 | non-lysosomal glucosylceramidase isoform X2 [Cucumis melo var. makuwa] | [more] |
| XP_008459592.1 | 0.0e+00 | 82.05 | PREDICTED: non-lysosomal glucosylceramidase isoform X2 [Cucumis melo] | [more] |
| Match Name | E-value | Identity | Description | |
|---|
| Q69ZF3 | 5.1e-139 | 36.72 | Non-lysosomal glucosylceramidase OS=Mus musculus OX=10090 GN=Gba2 PE=1 SV=2 | [more] |
| Q9HCG7 | 5.3e-136 | 36.87 | Non-lysosomal glucosylceramidase OS=Homo sapiens OX=9606 GN=GBA2 PE=1 SV=2 | [more] |
| Q5M868 | 6.9e-136 | 37.12 | Non-lysosomal glucosylceramidase OS=Rattus norvegicus OX=10116 GN=Gba2 PE=2 SV=2 | [more] |
| Q7KT91 | 4.2e-117 | 33.52 | Non-lysosomal glucosylceramidase OS=Drosophila melanogaster OX=7227 GN=CG33090 P... | [more] |
| Match Name | E-value | Identity | Description | |
|---|
| A0A0A0KXW4 | 0.0e+00 | 82.55 | Non-lysosomal glucosylceramidase OS=Cucumis sativus OX=3659 GN=Csa_5G649350 PE=3... | [more] |
| A0A5A7T726 | 0.0e+00 | 82.80 | Non-lysosomal glucosylceramidase OS=Cucumis melo var. makuwa OX=1194695 GN=E6C27... | [more] |
| A0A5D3BQT4 | 0.0e+00 | 82.80 | Non-lysosomal glucosylceramidase OS=Cucumis melo var. makuwa OX=1194695 GN=E5676... | [more] |
| A0A1S3CB21 | 0.0e+00 | 82.05 | Non-lysosomal glucosylceramidase OS=Cucumis melo OX=3656 GN=LOC103498676 PE=3 SV... | [more] |
| A0A1S4E2H5 | 0.0e+00 | 80.21 | Non-lysosomal glucosylceramidase OS=Cucumis melo OX=3656 GN=LOC103498676 PE=3 SV... | [more] |
