Cla97C07G144150 (gene) Watermelon (97103) v2.5

Overview
NameCla97C07G144150
Typegene
OrganismCitrullus lanatus subsp. vulgaris cv. 97103 (Watermelon (97103) v2.5)
DescriptionFe2OG dioxygenase domain-containing protein
LocationCla97Chr07: 31618873 .. 31620689 (-)
RNA-Seq ExpressionCla97C07G144150
SyntenyCla97C07G144150
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: polypeptideexonCDS
Hold the cursor over a type above to highlight its positions in the sequence below.
ATGGGTTTAAAGGGTGAAAGTTCAGGCACATTTCCCACAGGAGAGACTGCACAAGAGAAAGGCTTAAGCTATGTCCCTAATGCTTATGTGATTCCTGCCCCACACCGCCCGAGTTTGTCGCCTCAGATCGCTGTTGTTCCTGTCATTGACATGGCTTCTCTAAGATCAAATGATTCAGTTCAAAGGTCCCTTGCCATTGAAGAGCTCAGAAAAGCTTGCATTAGCCTAGGCTTCTTTCAGGTAAGGTTTTGCAAGGAACCGACTTACGTATACCCTACTTGTAGTTCTTCACTTTTTCGAATTTTTATATCAATTAGCTCTAAACTTAAATCCTCTTTTTGTTCAGATAATCAATCATGGAATCAGTGAAACAGTGATGGAAGAAGCACTCAACCAAGCCAATGAGTTCTTCAACTTACCACTAAAGGAGAAAATGAAGTATAAGTCGGATGATGTTTGCAAAGCAGTGAGGTATGGGACAAGCTTGAAAGATGGAGTGGATAAAATAAAGTTTTGGAGAGTCTTCCTCAAACAGTATGCTCATCCATTGGAAGATTGGATAGACTCCTGGCCTACCAATCCACCTAATTATAGGTAACTCAATATTTTAATTCTAATTTTCATTATTTTTCTCTTCCGAGAAAGGCTGTTCAAATAAGTTCAACAGAAAGCTACAATGAAACAATTGAACCGCAAAAAGAAAGCACCGCTATGTATATGCTGCCGCCTTTGAACCAGATTTCTAGCTGACAATGTTGCTTTTATATAGTTTTATATACCCTAACGTTTAATATATATATATATATGGTCTAAAAAAATTTGGATTTCTCTTTATTAATACATGTATACTCAGCATATAATATTGAGGCATTCTTCTTCAATGAACCATAAATAAAGGAGTCCAAATGCACACCAATAAATGACAATTTAAGAGAGCACTTTTTAGTCGCAGACAAGAGTTGGAGTAGATACCGACGAAAATATTGACGTCTTGAATGAAAATATGAGTATCAACTAATATTCTTAAAACAAATAAAAATAAATAAATATCACTGATCCCCCCCCCGCAGCCCCCAGAAAGAAACTATTATTTAATCTCTTGCCATAAGAAAACTACATATAAATTGAAATTTTATAAGAATCCTAGGTGCATATTTTCTTCAGAGATATGTCCTTGTGATTATAAGTAAGTATGTATATATATATACACATTATATATATATATGTACAACCGTTTCAGGGAAAAGATGGGACAATACACCAAGGAGGTGAGAAAAGTGAGCCTAGAGGTAATGGAAGCAATAACTGAAAGCTTGAGGTTAAGTCCAACATACCTCAGCGGAAAAATGGCTGAAGGAGTCCAAGTAGTGGCAGTAAACTGCTATCCGCCATGTCCACAGCCAGGGGTCGCACTTGGGTTGCCACCACATACAGACTACAGTTGCATAACCACAGTACTCCAAAGCAGCCAAGGCCTTGAAATCATGGACCCGGGTGATGGGTCATGGAAGATGGTCCCAAACCTTGATGGTGCTCTGCAGGTCCATGTGGGCGATCATGTGGAGGTTCTGAGCAATGGCCTTTACAAGGGAGTCGTCCACAGAGCTACTGTGAATTCTGAGAGAACAAGAATCTCCATTACCAGTTTGCACAGCTTGGGAATGGACGAGAAAATGAAGCCTGCAGAAGAGCTTGTGGATGAACAAAATCCTATGAAATATAAAGACAGCAGCTTCAATGATTTTCTGAAATTTCTGTCCAGCAACGATCTTGCTGAAGGAAAGAGCTTCATCAACACACTCAAAATCCAGGATTAA

mRNA sequence

ATGGGTTTAAAGGGTGAAAGTTCAGGCACATTTCCCACAGGAGAGACTGCACAAGAGAAAGGCTTAAGCTATGTCCCTAATGCTTATGTGATTCCTGCCCCACACCGCCCGAGTTTGTCGCCTCAGATCGCTGTTGTTCCTGTCATTGACATGGCTTCTCTAAGATCAAATGATTCAGTTCAAAGGTCCCTTGCCATTGAAGAGCTCAGAAAAGCTTGCATTAGCCTAGGCTTCTTTCAGATAATCAATCATGGAATCAGTGAAACAGTGATGGAAGAAGCACTCAACCAAGCCAATGAGTTCTTCAACTTACCACTAAAGGAGAAAATGAAGTATAAGTCGGATGATGTTTGCAAAGCAGTGAGGTATGGGACAAGCTTGAAAGATGGAGTGGATAAAATAAAGTTTTGGAGAGTCTTCCTCAAACAGTATGCTCATCCATTGGAAGATTGGATAGACTCCTGGCCTACCAATCCACCTAATTATAGGGAAAAGATGGGACAATACACCAAGGAGGTGAGAAAAGTGAGCCTAGAGGTAATGGAAGCAATAACTGAAAGCTTGAGGTTAAGTCCAACATACCTCAGCGGAAAAATGGCTGAAGGAGTCCAAGTAGTGGCAGTAAACTGCTATCCGCCATGTCCACAGCCAGGGGTCGCACTTGGGTTGCCACCACATACAGACTACAGTTGCATAACCACAGTACTCCAAAGCAGCCAAGGCCTTGAAATCATGGACCCGGGTGATGGGTCATGGAAGATGGTCCCAAACCTTGATGGTGCTCTGCAGGTCCATGTGGGCGATCATGTGGAGGTTCTGAGCAATGGCCTTTACAAGGGAGTCGTCCACAGAGCTACTGTGAATTCTGAGAGAACAAGAATCTCCATTACCAGTTTGCACAGCTTGGGAATGGACGAGAAAATGAAGCCTGCAGAAGAGCTTGTGGATGAACAAAATCCTATGAAATATAAAGACAGCAGCTTCAATGATTTTCTGAAATTTCTGTCCAGCAACGATCTTGCTGAAGGAAAGAGCTTCATCAACACACTCAAAATCCAGGATTAA

Coding sequence (CDS)

ATGGGTTTAAAGGGTGAAAGTTCAGGCACATTTCCCACAGGAGAGACTGCACAAGAGAAAGGCTTAAGCTATGTCCCTAATGCTTATGTGATTCCTGCCCCACACCGCCCGAGTTTGTCGCCTCAGATCGCTGTTGTTCCTGTCATTGACATGGCTTCTCTAAGATCAAATGATTCAGTTCAAAGGTCCCTTGCCATTGAAGAGCTCAGAAAAGCTTGCATTAGCCTAGGCTTCTTTCAGATAATCAATCATGGAATCAGTGAAACAGTGATGGAAGAAGCACTCAACCAAGCCAATGAGTTCTTCAACTTACCACTAAAGGAGAAAATGAAGTATAAGTCGGATGATGTTTGCAAAGCAGTGAGGTATGGGACAAGCTTGAAAGATGGAGTGGATAAAATAAAGTTTTGGAGAGTCTTCCTCAAACAGTATGCTCATCCATTGGAAGATTGGATAGACTCCTGGCCTACCAATCCACCTAATTATAGGGAAAAGATGGGACAATACACCAAGGAGGTGAGAAAAGTGAGCCTAGAGGTAATGGAAGCAATAACTGAAAGCTTGAGGTTAAGTCCAACATACCTCAGCGGAAAAATGGCTGAAGGAGTCCAAGTAGTGGCAGTAAACTGCTATCCGCCATGTCCACAGCCAGGGGTCGCACTTGGGTTGCCACCACATACAGACTACAGTTGCATAACCACAGTACTCCAAAGCAGCCAAGGCCTTGAAATCATGGACCCGGGTGATGGGTCATGGAAGATGGTCCCAAACCTTGATGGTGCTCTGCAGGTCCATGTGGGCGATCATGTGGAGGTTCTGAGCAATGGCCTTTACAAGGGAGTCGTCCACAGAGCTACTGTGAATTCTGAGAGAACAAGAATCTCCATTACCAGTTTGCACAGCTTGGGAATGGACGAGAAAATGAAGCCTGCAGAAGAGCTTGTGGATGAACAAAATCCTATGAAATATAAAGACAGCAGCTTCAATGATTTTCTGAAATTTCTGTCCAGCAACGATCTTGCTGAAGGAAAGAGCTTCATCAACACACTCAAAATCCAGGATTAA

Protein sequence

MGLKGESSGTFPTGETAQEKGLSYVPNAYVIPAPHRPSLSPQIAVVPVIDMASLRSNDSVQRSLAIEELRKACISLGFFQIINHGISETVMEEALNQANEFFNLPLKEKMKYKSDDVCKAVRYGTSLKDGVDKIKFWRVFLKQYAHPLEDWIDSWPTNPPNYREKMGQYTKEVRKVSLEVMEAITESLRLSPTYLSGKMAEGVQVVAVNCYPPCPQPGVALGLPPHTDYSCITTVLQSSQGLEIMDPGDGSWKMVPNLDGALQVHVGDHVEVLSNGLYKGVVHRATVNSERTRISITSLHSLGMDEKMKPAEELVDEQNPMKYKDSSFNDFLKFLSSNDLAEGKSFINTLKIQD
Homology
BLAST of Cla97C07G144150 vs. NCBI nr
Match: XP_004146935.2 (flavanone 3-dioxygenase 3 [Cucumis sativus] >KGN53184.2 hypothetical protein Csa_014422 [Cucumis sativus])

HSP 1 Score: 673.7 bits (1737), Expect = 8.6e-190
Identity = 327/354 (92.37%), Postives = 343/354 (96.89%), Query Frame = 0

Query: 1   MGLKGESSGTFPTGETAQEKGLSYVPNAYVIPAPHRPSLSPQIAVVPVIDMASLRSNDSV 60
           MGL GESSGTFPTG+TAQEKGLSYVPNAYVIPAPHRPS+SP+IA+VP+IDMASLRS+DSV
Sbjct: 3   MGLNGESSGTFPTGKTAQEKGLSYVPNAYVIPAPHRPSMSPEIAIVPIIDMASLRSSDSV 62

Query: 61  QRSLAIEELRKACISLGFFQIINHGISETVMEEALNQANEFFNLPLKEKMKYKSDDVCKA 120
           QRSLA EELRKACISLGFFQIINHGI+ETVMEEAL+QANEFFNLPLKEKMKYKSDDVCKA
Sbjct: 63  QRSLATEELRKACISLGFFQIINHGITETVMEEALSQANEFFNLPLKEKMKYKSDDVCKA 122

Query: 121 VRYGTSLKDGVDKIKFWRVFLKQYAHPLEDWIDSWPTNPPNYREKMGQYTKEVRKVSLEV 180
           VRYGTSLKDGVDKIKFWRVFLK YAHPLEDWIDSWPTNP NYREKMG+YTKEVRKVSLE+
Sbjct: 123 VRYGTSLKDGVDKIKFWRVFLKHYAHPLEDWIDSWPTNPRNYREKMGRYTKEVRKVSLEI 182

Query: 181 MEAITESLRLSPTYLSGKMAEGVQVVAVNCYPPCPQPGVALGLPPHTDYSCITTVLQSSQ 240
           MEAI ESL LSPTYLS KMAEGVQVVAVNCYPPCPQPGVALGLPPHTDYSCITTVLQSSQ
Sbjct: 183 MEAINESLNLSPTYLSSKMAEGVQVVAVNCYPPCPQPGVALGLPPHTDYSCITTVLQSSQ 242

Query: 241 GLEIMDPGDGSWKMVPNLDGALQVHVGDHVEVLSNGLYKGVVHRATVNSERTRISITSLH 300
           GLE+MDP DG+WKMVP +DGALQVHVGDHVEVLSNGLYKGVVHRATVNSERTRISITSLH
Sbjct: 243 GLEVMDPADGTWKMVPKIDGALQVHVGDHVEVLSNGLYKGVVHRATVNSERTRISITSLH 302

Query: 301 SLGMDEKMKPAEELVDEQNPMKYKDSSFNDFLKFLSSNDLAEGKSFINTLKIQD 355
           SLGMDEKMKPAEELV+E NP KYKDSSFNDFLKFLSSNDLAEGKSF+NTLK+QD
Sbjct: 303 SLGMDEKMKPAEELVNEHNPKKYKDSSFNDFLKFLSSNDLAEGKSFLNTLKLQD 356

BLAST of Cla97C07G144150 vs. NCBI nr
Match: XP_008453850.1 (PREDICTED: protein DOWNY MILDEW RESISTANCE 6 [Cucumis melo] >KAA0044683.1 protein DOWNY MILDEW RESISTANCE 6 [Cucumis melo var. makuwa] >TYK16900.1 protein DOWNY MILDEW RESISTANCE 6 [Cucumis melo var. makuwa])

HSP 1 Score: 672.5 bits (1734), Expect = 1.9e-189
Identity = 328/354 (92.66%), Postives = 342/354 (96.61%), Query Frame = 0

Query: 1   MGLKGESSGTFPTGETAQEKGLSYVPNAYVIPAPHRPSLSPQIAVVPVIDMASLRSNDSV 60
           MGL GE SGTFPTG+TAQEKGLSYVPNAYVIPAPHRPSLSP+IA+VP+IDMASLRSNDSV
Sbjct: 1   MGLNGEGSGTFPTGKTAQEKGLSYVPNAYVIPAPHRPSLSPEIAIVPIIDMASLRSNDSV 60

Query: 61  QRSLAIEELRKACISLGFFQIINHGISETVMEEALNQANEFFNLPLKEKMKYKSDDVCKA 120
           QRSLA+EELRKACISLGFFQIINHGI+ETVMEEAL+QANEFFNLPL EKMKYKSDDVCKA
Sbjct: 61  QRSLAMEELRKACISLGFFQIINHGITETVMEEALSQANEFFNLPLTEKMKYKSDDVCKA 120

Query: 121 VRYGTSLKDGVDKIKFWRVFLKQYAHPLEDWIDSWPTNPPNYREKMGQYTKEVRKVSLEV 180
           VRYGTSLKDG+DKIKFWRVFLK YAHPLEDWIDSWPTNP NYREKMGQYTKEVRKVSLE+
Sbjct: 121 VRYGTSLKDGIDKIKFWRVFLKHYAHPLEDWIDSWPTNPRNYREKMGQYTKEVRKVSLEI 180

Query: 181 MEAITESLRLSPTYLSGKMAEGVQVVAVNCYPPCPQPGVALGLPPHTDYSCITTVLQSSQ 240
           MEAITESL LSPTYLS KMAEGVQVVAVNCYPPCPQPGVALGLPPHTDYSCITTVLQ+SQ
Sbjct: 181 MEAITESLNLSPTYLSSKMAEGVQVVAVNCYPPCPQPGVALGLPPHTDYSCITTVLQNSQ 240

Query: 241 GLEIMDPGDGSWKMVPNLDGALQVHVGDHVEVLSNGLYKGVVHRATVNSERTRISITSLH 300
           GLEIMDP DG+WKMV  +DGALQVHVGDHVEVLSNGLYKGVVHRATVNSERTRISITSLH
Sbjct: 241 GLEIMDPADGTWKMVQKIDGALQVHVGDHVEVLSNGLYKGVVHRATVNSERTRISITSLH 300

Query: 301 SLGMDEKMKPAEELVDEQNPMKYKDSSFNDFLKFLSSNDLAEGKSFINTLKIQD 355
           SLGMDEKMKPAEELV+EQNP KYK+SSFNDFLKFLSSNDLAEG SFINTLKIQD
Sbjct: 301 SLGMDEKMKPAEELVNEQNPKKYKESSFNDFLKFLSSNDLAEGNSFINTLKIQD 354

BLAST of Cla97C07G144150 vs. NCBI nr
Match: XP_038889497.1 (flavanone 3-dioxygenase 3 [Benincasa hispida])

HSP 1 Score: 653.3 bits (1684), Expect = 1.2e-183
Identity = 324/358 (90.50%), Postives = 337/358 (94.13%), Query Frame = 0

Query: 1   MGLKGESSGT----FPTGETAQEKGLSYVPNAYVIPAPHRPSLSPQIAVVPVIDMASLRS 60
           M   G+SSGT    FPTG+TAQEKG SYVPNAYVIPAPHR SLSP+IA VP+IDMASLRS
Sbjct: 1   MEFNGQSSGTGTGSFPTGKTAQEKGFSYVPNAYVIPAPHRASLSPEIATVPIIDMASLRS 60

Query: 61  NDSVQRSLAIEELRKACISLGFFQIINHGISETVMEEALNQANEFFNLPLKEKMKYKSDD 120
           NDS QRSLAI+ELRKACISLGFFQIINHGISETVMEEALNQANEFFNLPLKEKMKYKSDD
Sbjct: 61  NDSAQRSLAIQELRKACISLGFFQIINHGISETVMEEALNQANEFFNLPLKEKMKYKSDD 120

Query: 121 VCKAVRYGTSLKDGVDKIKFWRVFLKQYAHPLEDWIDSWPTNPPNYREKMGQYTKEVRKV 180
           V KAVRYGTSLKDGVDKIKFWRVFLK YAHPLEDWIDSWPTNP NYREKMGQYTK VRKV
Sbjct: 121 VRKAVRYGTSLKDGVDKIKFWRVFLKHYAHPLEDWIDSWPTNPRNYREKMGQYTKVVRKV 180

Query: 181 SLEVMEAITESLRLSPTYLSGKMAEGVQVVAVNCYPPCPQPGVALGLPPHTDYSCITTVL 240
           SLE+MEAITESL LSPTYLS KMAEGVQVVAVNCYPPCPQPGVALGLPPHTDYSCITTVL
Sbjct: 181 SLEIMEAITESLSLSPTYLSSKMAEGVQVVAVNCYPPCPQPGVALGLPPHTDYSCITTVL 240

Query: 241 QSSQGLEIMDPGDGSWKMVPNLDGALQVHVGDHVEVLSNGLYKGVVHRATVNSERTRISI 300
           QS+QGLEIMDP DG+WKMVP + GALQVH+GDHVEVLSNGLYKGVVHRATVNSER RISI
Sbjct: 241 QSNQGLEIMDPADGTWKMVPKIAGALQVHLGDHVEVLSNGLYKGVVHRATVNSERARISI 300

Query: 301 TSLHSLGMDEKMKPAEELVDEQNPMKYKDSSFNDFLKFLSSNDLAEGKSFINTLKIQD 355
           TSLHSLGMDEKMKPAEELVDEQNP+KYKDSSF+DFLKFLSSNDLAEGKSFINTLKI+D
Sbjct: 301 TSLHSLGMDEKMKPAEELVDEQNPIKYKDSSFDDFLKFLSSNDLAEGKSFINTLKIED 358

BLAST of Cla97C07G144150 vs. NCBI nr
Match: XP_022922584.1 (LOW QUALITY PROTEIN: flavanone 3-dioxygenase 3 [Cucurbita moschata])

HSP 1 Score: 636.7 bits (1641), Expect = 1.2e-178
Identity = 318/376 (84.57%), Postives = 334/376 (88.83%), Query Frame = 0

Query: 1   MGLKGESSGTFPTGETAQEKGLSYVPNAYVIPAPHRPSLSPQIAVVPVIDMASLRSNDSV 60
           MGLKGESSG+F TG+TAQEKGL+YVPNAYVIP P RPSLSP+ A+VP+IDMASLRSNDS 
Sbjct: 1   MGLKGESSGSFTTGQTAQEKGLNYVPNAYVIPTPQRPSLSPETAIVPIIDMASLRSNDSA 60

Query: 61  QRSLAIEELRKACISLGFF----------------------QIINHGISETVMEEALNQA 120
           QRSL IE LRKACISLGFF                      QIINHGIS+TVMEEALNQA
Sbjct: 61  QRSLTIEALRKACISLGFFQVKFQATYLFYISXLLTSICFLQIINHGISKTVMEEALNQA 120

Query: 121 NEFFNLPLKEKMKYKSDDVCKAVRYGTSLKDGVDKIKFWRVFLKQYAHPLEDWIDSWPTN 180
            EFFNLPLKEKMKYKSDDV KAVRYGTSLKDGVDKIKFWRVFLK YAHPLEDW+DSWPTN
Sbjct: 121 REFFNLPLKEKMKYKSDDVSKAVRYGTSLKDGVDKIKFWRVFLKHYAHPLEDWVDSWPTN 180

Query: 181 PPNYREKMGQYTKEVRKVSLEVMEAITESLRLSPTYLSGKMAEGVQVVAVNCYPPCPQPG 240
           P  YREKMGQYTKEVRKVSLE+ EAITESL L PTYLS KMAEGVQVVAVNCYPPCPQPG
Sbjct: 181 PHTYREKMGQYTKEVRKVSLEIEEAITESLGLRPTYLSSKMAEGVQVVAVNCYPPCPQPG 240

Query: 241 VALGLPPHTDYSCITTVLQSSQGLEIMDPGDGSWKMVPNLDGALQVHVGDHVEVLSNGLY 300
           VALGLPPHTDYSCITT+LQSSQGLEIMD  DG WKMVPN+DG+LQVHVGDHVEVLSNGLY
Sbjct: 241 VALGLPPHTDYSCITTILQSSQGLEIMDRADGKWKMVPNVDGSLQVHVGDHVEVLSNGLY 300

Query: 301 KGVVHRATVNSERTRISITSLHSLGMDEKMKPAEELVDEQNPMKYKDSSFNDFLKFLSSN 355
           KGVVHRA VNSERTRISI+SLHSLGMDEKMKPAEELVDEQNP+KYK+SSFNDFLKFLSSN
Sbjct: 301 KGVVHRAIVNSERTRISISSLHSLGMDEKMKPAEELVDEQNPIKYKESSFNDFLKFLSSN 360

BLAST of Cla97C07G144150 vs. NCBI nr
Match: XP_023552054.1 (LOW QUALITY PROTEIN: flavanone 3-dioxygenase 3 [Cucurbita pepo subsp. pepo])

HSP 1 Score: 636.0 bits (1639), Expect = 2.0e-178
Identity = 317/379 (83.64%), Postives = 336/379 (88.65%), Query Frame = 0

Query: 1   MGLKGESSGTFPTGETAQEKGLSYVPNAYVIPAPHRPSLSPQIAVVPVIDMASLRSNDSV 60
           MGL GESSG+FPTG+TAQEKGL+YVPNAYVIP P RPSLSP+ A+VP+IDMASLRS+DS 
Sbjct: 62  MGLGGESSGSFPTGQTAQEKGLNYVPNAYVIPTPQRPSLSPETAIVPIIDMASLRSDDSA 121

Query: 61  QRSLAIEELRKACISLGFF-------------------------QIINHGISETVMEEAL 120
           QRSLAIE LRKACIS+GFF                         QIINHGIS+TVMEEAL
Sbjct: 122 QRSLAIEVLRKACISIGFFQVKFQATYLPTSFILASSXLQFVFLQIINHGISKTVMEEAL 181

Query: 121 NQANEFFNLPLKEKMKYKSDDVCKAVRYGTSLKDGVDKIKFWRVFLKQYAHPLEDWIDSW 180
           +QA EFFNLPLKEKMKYKSDDV KAVRYGTSLKDGVDKIKFWRVFLK YAHPLEDW+DSW
Sbjct: 182 SQAREFFNLPLKEKMKYKSDDVSKAVRYGTSLKDGVDKIKFWRVFLKHYAHPLEDWVDSW 241

Query: 181 PTNPPNYREKMGQYTKEVRKVSLEVMEAITESLRLSPTYLSGKMAEGVQVVAVNCYPPCP 240
           PTNP  YREKMGQYTKEVRKVSLE+ EAITESL L PTYLS KMAEGVQVVAVNCYPPCP
Sbjct: 242 PTNPHTYREKMGQYTKEVRKVSLEIEEAITESLGLRPTYLSSKMAEGVQVVAVNCYPPCP 301

Query: 241 QPGVALGLPPHTDYSCITTVLQSSQGLEIMDPGDGSWKMVPNLDGALQVHVGDHVEVLSN 300
           QPGVALGLPPHTDYSCITT+LQSSQGLEIMDP DG WKMVPN+DG+LQVHVGDHVEVLSN
Sbjct: 302 QPGVALGLPPHTDYSCITTILQSSQGLEIMDPADGKWKMVPNVDGSLQVHVGDHVEVLSN 361

Query: 301 GLYKGVVHRATVNSERTRISITSLHSLGMDEKMKPAEELVDEQNPMKYKDSSFNDFLKFL 355
           GLYKGVVHRA VNSERTRISI+SLHSLGMDEKMKPAEELVDEQNP+KYK+SSFNDFLKFL
Sbjct: 362 GLYKGVVHRAIVNSERTRISISSLHSLGMDEKMKPAEELVDEQNPIKYKESSFNDFLKFL 421

BLAST of Cla97C07G144150 vs. ExPASy Swiss-Prot
Match: Q7XR84 (Flavanone 3-dioxygenase 3 OS=Oryza sativa subsp. japonica OX=39947 GN=F3H-3 PE=1 SV=1)

HSP 1 Score: 311.2 bits (796), Expect = 1.5e-83
Identity = 155/322 (48.14%), Postives = 213/322 (66.15%), Query Frame = 0

Query: 31  IPAPHRPSLSPQIAVVPVIDMASLRSNDSVQRSLAIEELRKACISLGFFQIINHGISETV 90
           IP    PS       +PVI++  L  +D   RS  + ++ KAC  LG+FQ+I+HGIS++V
Sbjct: 8   IPQEQLPSQELHPPPMPVINLGHLSLDDPTVRSRVVNDIAKACRDLGYFQVISHGISQSV 67

Query: 91  MEEALNQANEFFNLPLKEKMKYKSDDVCKAVRYGTSLKDGVDKIKFWRVFLKQYAHPLED 150
           M+ A+  A+EFF LP + K +Y SDD+ + VRY TS KDG   I   R FLK YAHPL D
Sbjct: 68  MDGAIEAASEFFKLPNEIKKEYASDDIRQPVRYDTSSKDG---ISMSRAFLKHYAHPLCD 127

Query: 151 WIDSWPTNPPNYREKMGQYTKEVRKVSLEVMEAITESLRLSPTYLSGKMAEGVQVVAVNC 210
           W+  WP  PP YRE M +Y  EVR V+L++MEAI E L +   Y+  K  EG+Q+++VNC
Sbjct: 128 WLQYWPQQPPIYREYMAKYAVEVRVVALKLMEAILEGLGIGKEYMQEKFEEGLQLLSVNC 187

Query: 211 YPPCPQPGVALGLPPHTDYSCITTVLQSSQGLEIMDPGDGSWKMVPNLDGALQVHVGDHV 270
           YP   Q   ++GL  H+DY  +T +L S QGLE++D    SWK+V  L  AL VHVGDH+
Sbjct: 188 YPKVSQSDTSIGLAAHSDYGLLTILLTSCQGLEVVDRSSNSWKVVQQLPHALHVHVGDHM 247

Query: 271 EVLSNGLYKGVVHRATVNSERTRISITSLHSLGMDEKMKPAEELVDEQNPMKYKDSSFND 330
           EVLSNG  K VVHRA +N +  RIS+ S+H   + EK+  A+ELVDE+NP KYK++SFND
Sbjct: 248 EVLSNGRIKTVVHRAVLNPQEARISLASIHGFALHEKVSSAKELVDEENPQKYKENSFND 307

Query: 331 FLKFLSSNDLAEGKSFINTLKI 353
           FL+ L++N     ++F+ +L++
Sbjct: 308 FLEHLTANMDNRQRNFLESLRM 326

BLAST of Cla97C07G144150 vs. ExPASy Swiss-Prot
Match: Q7XR83 (2-oxoglutarate-dependent dioxygenase 21, chloroplastic OS=Oryza sativa subsp. japonica OX=39947 GN=2ODD21 PE=1 SV=1)

HSP 1 Score: 298.9 bits (764), Expect = 7.6e-80
Identity = 155/317 (48.90%), Postives = 210/317 (66.25%), Query Frame = 0

Query: 37  PSLSPQIAVVPVIDMASLRSNDSVQRSLAIEELRKACISLGFFQIINHGISETVMEEALN 96
           P L P    +PVI++  L + DS  R+LA+ ++  AC   G F+++NHGIS + M  AL 
Sbjct: 19  PPLPPPPRPLPVINLGRL-TMDSASRALAVRDIVLACRERGCFEVVNHGISRSCMNGALE 78

Query: 97  QANEFFNLPLKEKMKYKSDDVCKAVRYGTSLKDGVDKIKFWRVFLKQYAHPLEDWIDSWP 156
            A+EFF L  + K ++ SDD+ + +RY TS +DG   I   R FLK YA+PL+DWI  WP
Sbjct: 79  AASEFFQLSTERKEEFASDDIRQPIRYDTSSRDG---ISMSRSFLKHYANPLDDWIKFWP 138

Query: 157 TNPPNYREKMGQYTKEVRKVSLEVMEAITESLRLSPTYLSGKMAEGVQVVAVNCYP-PCP 216
           T PP YREKMG+Y  E ++VS+++MEAI + L L P+YL  K+  GVQ VA+N YP    
Sbjct: 139 TQPPTYREKMGEYAVETQRVSMQLMEAILQGLGLGPSYLQEKLEGGVQFVALNNYPQSSA 198

Query: 217 QPGVALGLPPHTDYSCITTVLQSSQGLEIMDPGDGSWKMVPNLDGALQVHVGDHVEVLSN 276
           +    +GL PH+DY  +T +LQSS GLE+M   D +W  VP + GAL VHVGDH+EVLSN
Sbjct: 199 KKADKIGLAPHSDYGFLTILLQSSPGLEVMHHEDDAWTSVPAIPGALHVHVGDHLEVLSN 258

Query: 277 GLYKGVVHRATVNSERTRISITSLHSLGMDEKMKPAEELVDEQNPMKYKDSSFNDFLKFL 336
           G  K +VHRA +N   +RISI S+H L MDE++  AEELVDE +P  Y+ SSF DFL FL
Sbjct: 259 GQLKSLVHRAVLNPNESRISIASIHGLSMDEEVHCAEELVDEHHPKMYRGSSFQDFLDFL 318

Query: 337 SSNDLAEGKSFINTLKI 353
            +N +   K ++ +L+I
Sbjct: 319 PAN-MNRYKRYVESLRI 330

BLAST of Cla97C07G144150 vs. ExPASy Swiss-Prot
Match: Q6YYX9 (Probable 2-oxoglutarate-dependent dioxygenase SLC1 OS=Oryza sativa subsp. japonica OX=39947 GN=SLC1 PE=2 SV=1)

HSP 1 Score: 257.3 bits (656), Expect = 2.5e-67
Identity = 136/342 (39.77%), Postives = 202/342 (59.06%), Query Frame = 0

Query: 19  EKGLSYVPNAYVIPAPHRPSLSPQIAV-------VPVIDMASLRSNDSVQRSLAIEELRK 78
           + G++ +P  YV+PA  RP  +   A        +PV+D++ LR     +R   +  L  
Sbjct: 46  DSGITRLPGNYVLPASDRPGQAAGAAAAAGGSVKLPVVDLSRLRVPS--ERGAVLRTLDA 105

Query: 79  ACISLGFFQIINHGISETVMEEALNQANEFFNLPLKEKMKYKSDDVCKAVRYGTSLKDGV 138
           AC   GFFQ++NHG+   V+   L+ A  FF LP  E+ +Y S DV   VRYGTS     
Sbjct: 106 ACREYGFFQVVNHGVGGEVVGGMLDVARRFFELPQPERERYMSADVRAPVRYGTSFNQVR 165

Query: 139 DKIKFWRVFLKQYAHPLEDWIDSWPTNPPNYREKMGQYTKEVRKVSLEVMEAITESLRLS 198
           D +  WR FLK    PL   ++SWPT+P + RE   +Y +  ++V +EVMEA  E+L + 
Sbjct: 166 DAVLCWRDFLKLACMPLAAVVESWPTSPADLREVASRYAEANQRVFMEVMEAALEALGVG 225

Query: 199 PTYLSGKMAEGVQVVAVNCYPPCPQPGVALGLPPHTDYSCITTVLQSS-QGLEIMDPGDG 258
              +   +A G Q++ VNCYP CPQP + LG+PPH+DY  +T VLQ    GL++M  G+ 
Sbjct: 226 GGGVMEDLAAGTQMMTVNCYPECPQPELTLGMPPHSDYGFLTLVLQDEVAGLQVMHAGE- 285

Query: 259 SWKMVPNLDGALQVHVGDHVEVLSNGLYKGVVHRATVNSERTRISITSLHSLGMDEKMKP 318
            W  V  L G+  V+VGDH+E+LSNG Y+ V+HR  VNS R R+S+ S HS+  +  + P
Sbjct: 286 -WLTVDPLPGSFVVNVGDHLEILSNGRYRSVLHRVKVNSRRLRVSVASFHSVAPERVVSP 345

Query: 319 AEELVDEQNPMKYKDSSFNDFLKFLSSNDLAEGKSFINTLKI 353
           A EL+D+++P +Y D+    FL +L+S      KSF+++ ++
Sbjct: 346 APELIDDRHPRRYMDTDLATFLAYLAS-AAGNHKSFLHSRRL 382

BLAST of Cla97C07G144150 vs. ExPASy Swiss-Prot
Match: Q651B6 (Probable 2-oxoglutarate-dependent dioxygenase SLC2 OS=Oryza sativa subsp. japonica OX=39947 GN=SLC2 PE=2 SV=1)

HSP 1 Score: 237.7 bits (605), Expect = 2.1e-61
Identity = 139/346 (40.17%), Postives = 204/346 (58.96%), Query Frame = 0

Query: 19  EKGLSYVPNAYVIPAPHRPS--LSPQIAVVPVIDMASLRSNDSVQRSLAIEELRKACISL 78
           + G++ +P  YV+P   RP+  ++P  A++PV+D+A+LR+ D  Q    +  L  AC   
Sbjct: 30  DSGITTLPARYVLPPADRPARYVTPP-ALLPVVDLAALRARDPCQ----LAALHAACRDY 89

Query: 79  GFFQIINHGISETVMEEALNQANEFFNLPLKEKMKYKSDDVCKAVRYGTSLKDGVDKIKF 138
           GFFQ++NHG+    M  A  +   FF+LPL  + +Y S D+  AVRYGTS     D +  
Sbjct: 90  GFFQLLNHGVPPDAMLYAARRF--FFDLPLPARKRYMSADIRAAVRYGTSFNQLNDAVLS 149

Query: 139 WRVFLK---QYAHPLEDWIDSWPTNPPNYREKMGQYTKEVRKVSLEVMEAITESLRL--S 198
           WR FLK   +    L D + SWP  P + R     Y    +++  E+MEA  ++L +   
Sbjct: 150 WRDFLKLLIRDTRRLADVLPSWPDAPDDLRPAAAAYATACQRLFRELMEAALDALGIVRC 209

Query: 199 PTYLSGKMAEGVQVVAVNCYPPCPQPGVALGLPPHTDYSCITTVLQSS-QGLEIMDPGDG 258
              L  +   G Q++ VNC+P CP+P + LG+PPH+DY  +T +LQ   +GLE+     G
Sbjct: 210 RRQLLEECDAGSQMMMVNCFPACPEPELTLGMPPHSDYGLLTILLQDEVRGLEVSYGDGG 269

Query: 259 SWKMVPNLDGALQVHVGDHVEVLSNGLYKGVVHRATVNSERTRISITSLHSLGMDEKMKP 318
            W +V  L GA+ V+VGDH+E+LSNGLY+ V+HR  VN  R R+S+ SLHSL  +  + P
Sbjct: 270 GWAVVEPLPGAVVVNVGDHLEILSNGLYRSVLHRVRVNGRRARVSVASLHSLAAERVIGP 329

Query: 319 AEELVDEQ--NPMKYKDSSFNDFLKFLSSNDLAEG--KSFINTLKI 353
           A ELVDEQ   P +Y D+    FL +L+S   AEG  KSF+++ +I
Sbjct: 330 AAELVDEQRGRPRRYMDTDMAAFLAYLAS---AEGNHKSFLHSRRI 365

BLAST of Cla97C07G144150 vs. ExPASy Swiss-Prot
Match: Q9FLV0 (Protein DOWNY MILDEW RESISTANCE 6 OS=Arabidopsis thaliana OX=3702 GN=DMR6 PE=1 SV=1)

HSP 1 Score: 228.4 bits (581), Expect = 1.3e-58
Identity = 121/333 (36.34%), Postives = 195/333 (58.56%), Query Frame = 0

Query: 25  VPNAYVIPAPHRPSLS--PQIAVVPVIDMASLRSNDSVQRSLAIEELRKACISLGFFQII 84
           +P  YV P   RP LS   Q+   P+ID++      S  RS  I+++ +AC   GFFQ+I
Sbjct: 15  LPENYVRPISDRPRLSEVSQLEDFPLIDLS------STDRSFLIQQIHQACARFGFFQVI 74

Query: 85  NHGISETVMEEALNQANEFFNLPLKEKMKYKSDDVCKAVRYGTSLKDGVDKIKFWRVFLK 144
           NHG+++ +++E ++ A EFF++ ++EKMK  SDD  K  R  TS     +++  WR +L+
Sbjct: 75  NHGVNKQIIDEMVSVAREFFSMSMEEKMKLYSDDPTKTTRLSTSFNVKKEEVNNWRDYLR 134

Query: 145 QYAHPLEDWIDSWPTNPPNYREKMGQYTKEVRKVSLEVMEAITESLRLSPTYLSGKMAEG 204
            + +P+  +++ WP+NPP+++E + +Y++EVR+V  ++ E I+ESL L   Y+   + E 
Sbjct: 135 LHCYPIHKYVNEWPSNPPSFKEIVSKYSREVREVGFKIEELISESLGLEKDYMKKVLGEQ 194

Query: 205 VQVVAVNCYPPCPQPGVALGLPPHTDYSCITTVLQSSQ--GLEIMDPGDGSWKMVPNLDG 264
            Q +AVN YPPCP+P +  GLP HTD + +T +LQ +   GL+I+   DG W  V     
Sbjct: 195 GQHMAVNYYPPCPEPELTYGLPAHTDPNALTILLQDTTVCGLQILI--DGQWFAVNPHPD 254

Query: 265 ALQVHVGDHVEVLSNGLYKGVVHRATVNSERTRISITSL---HSLGMDEKMKPAEELVDE 324
           A  +++GD ++ LSNG+YK V HRA  N+E  R+S+ S        +    KP  E  D+
Sbjct: 255 AFVINIGDQLQALSNGVYKSVWHRAVTNTENPRLSVASFLCPADCAVMSPAKPLWEAEDD 314

Query: 325 QNPMKYKDSSFNDFLKFLSSNDLAEGKSFINTL 351
           +    YKD ++ ++ K   S +L +     N L
Sbjct: 315 ETKPVYKDFTYAEYYKKFWSRNLDQEHCLENFL 339

BLAST of Cla97C07G144150 vs. ExPASy TrEMBL
Match: A0A5A7TMH3 (Protein DOWNY MILDEW RESISTANCE 6 OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold130G00340 PE=3 SV=1)

HSP 1 Score: 672.5 bits (1734), Expect = 9.3e-190
Identity = 328/354 (92.66%), Postives = 342/354 (96.61%), Query Frame = 0

Query: 1   MGLKGESSGTFPTGETAQEKGLSYVPNAYVIPAPHRPSLSPQIAVVPVIDMASLRSNDSV 60
           MGL GE SGTFPTG+TAQEKGLSYVPNAYVIPAPHRPSLSP+IA+VP+IDMASLRSNDSV
Sbjct: 1   MGLNGEGSGTFPTGKTAQEKGLSYVPNAYVIPAPHRPSLSPEIAIVPIIDMASLRSNDSV 60

Query: 61  QRSLAIEELRKACISLGFFQIINHGISETVMEEALNQANEFFNLPLKEKMKYKSDDVCKA 120
           QRSLA+EELRKACISLGFFQIINHGI+ETVMEEAL+QANEFFNLPL EKMKYKSDDVCKA
Sbjct: 61  QRSLAMEELRKACISLGFFQIINHGITETVMEEALSQANEFFNLPLTEKMKYKSDDVCKA 120

Query: 121 VRYGTSLKDGVDKIKFWRVFLKQYAHPLEDWIDSWPTNPPNYREKMGQYTKEVRKVSLEV 180
           VRYGTSLKDG+DKIKFWRVFLK YAHPLEDWIDSWPTNP NYREKMGQYTKEVRKVSLE+
Sbjct: 121 VRYGTSLKDGIDKIKFWRVFLKHYAHPLEDWIDSWPTNPRNYREKMGQYTKEVRKVSLEI 180

Query: 181 MEAITESLRLSPTYLSGKMAEGVQVVAVNCYPPCPQPGVALGLPPHTDYSCITTVLQSSQ 240
           MEAITESL LSPTYLS KMAEGVQVVAVNCYPPCPQPGVALGLPPHTDYSCITTVLQ+SQ
Sbjct: 181 MEAITESLNLSPTYLSSKMAEGVQVVAVNCYPPCPQPGVALGLPPHTDYSCITTVLQNSQ 240

Query: 241 GLEIMDPGDGSWKMVPNLDGALQVHVGDHVEVLSNGLYKGVVHRATVNSERTRISITSLH 300
           GLEIMDP DG+WKMV  +DGALQVHVGDHVEVLSNGLYKGVVHRATVNSERTRISITSLH
Sbjct: 241 GLEIMDPADGTWKMVQKIDGALQVHVGDHVEVLSNGLYKGVVHRATVNSERTRISITSLH 300

Query: 301 SLGMDEKMKPAEELVDEQNPMKYKDSSFNDFLKFLSSNDLAEGKSFINTLKIQD 355
           SLGMDEKMKPAEELV+EQNP KYK+SSFNDFLKFLSSNDLAEG SFINTLKIQD
Sbjct: 301 SLGMDEKMKPAEELVNEQNPKKYKESSFNDFLKFLSSNDLAEGNSFINTLKIQD 354

BLAST of Cla97C07G144150 vs. ExPASy TrEMBL
Match: A0A1S3BX86 (protein DOWNY MILDEW RESISTANCE 6 OS=Cucumis melo OX=3656 GN=LOC103494453 PE=3 SV=1)

HSP 1 Score: 672.5 bits (1734), Expect = 9.3e-190
Identity = 328/354 (92.66%), Postives = 342/354 (96.61%), Query Frame = 0

Query: 1   MGLKGESSGTFPTGETAQEKGLSYVPNAYVIPAPHRPSLSPQIAVVPVIDMASLRSNDSV 60
           MGL GE SGTFPTG+TAQEKGLSYVPNAYVIPAPHRPSLSP+IA+VP+IDMASLRSNDSV
Sbjct: 1   MGLNGEGSGTFPTGKTAQEKGLSYVPNAYVIPAPHRPSLSPEIAIVPIIDMASLRSNDSV 60

Query: 61  QRSLAIEELRKACISLGFFQIINHGISETVMEEALNQANEFFNLPLKEKMKYKSDDVCKA 120
           QRSLA+EELRKACISLGFFQIINHGI+ETVMEEAL+QANEFFNLPL EKMKYKSDDVCKA
Sbjct: 61  QRSLAMEELRKACISLGFFQIINHGITETVMEEALSQANEFFNLPLTEKMKYKSDDVCKA 120

Query: 121 VRYGTSLKDGVDKIKFWRVFLKQYAHPLEDWIDSWPTNPPNYREKMGQYTKEVRKVSLEV 180
           VRYGTSLKDG+DKIKFWRVFLK YAHPLEDWIDSWPTNP NYREKMGQYTKEVRKVSLE+
Sbjct: 121 VRYGTSLKDGIDKIKFWRVFLKHYAHPLEDWIDSWPTNPRNYREKMGQYTKEVRKVSLEI 180

Query: 181 MEAITESLRLSPTYLSGKMAEGVQVVAVNCYPPCPQPGVALGLPPHTDYSCITTVLQSSQ 240
           MEAITESL LSPTYLS KMAEGVQVVAVNCYPPCPQPGVALGLPPHTDYSCITTVLQ+SQ
Sbjct: 181 MEAITESLNLSPTYLSSKMAEGVQVVAVNCYPPCPQPGVALGLPPHTDYSCITTVLQNSQ 240

Query: 241 GLEIMDPGDGSWKMVPNLDGALQVHVGDHVEVLSNGLYKGVVHRATVNSERTRISITSLH 300
           GLEIMDP DG+WKMV  +DGALQVHVGDHVEVLSNGLYKGVVHRATVNSERTRISITSLH
Sbjct: 241 GLEIMDPADGTWKMVQKIDGALQVHVGDHVEVLSNGLYKGVVHRATVNSERTRISITSLH 300

Query: 301 SLGMDEKMKPAEELVDEQNPMKYKDSSFNDFLKFLSSNDLAEGKSFINTLKIQD 355
           SLGMDEKMKPAEELV+EQNP KYK+SSFNDFLKFLSSNDLAEG SFINTLKIQD
Sbjct: 301 SLGMDEKMKPAEELVNEQNPKKYKESSFNDFLKFLSSNDLAEGNSFINTLKIQD 354

BLAST of Cla97C07G144150 vs. ExPASy TrEMBL
Match: A0A6J1E3T8 (LOW QUALITY PROTEIN: flavanone 3-dioxygenase 3 OS=Cucurbita moschata OX=3662 GN=LOC111430549 PE=3 SV=1)

HSP 1 Score: 636.7 bits (1641), Expect = 5.7e-179
Identity = 318/376 (84.57%), Postives = 334/376 (88.83%), Query Frame = 0

Query: 1   MGLKGESSGTFPTGETAQEKGLSYVPNAYVIPAPHRPSLSPQIAVVPVIDMASLRSNDSV 60
           MGLKGESSG+F TG+TAQEKGL+YVPNAYVIP P RPSLSP+ A+VP+IDMASLRSNDS 
Sbjct: 1   MGLKGESSGSFTTGQTAQEKGLNYVPNAYVIPTPQRPSLSPETAIVPIIDMASLRSNDSA 60

Query: 61  QRSLAIEELRKACISLGFF----------------------QIINHGISETVMEEALNQA 120
           QRSL IE LRKACISLGFF                      QIINHGIS+TVMEEALNQA
Sbjct: 61  QRSLTIEALRKACISLGFFQVKFQATYLFYISXLLTSICFLQIINHGISKTVMEEALNQA 120

Query: 121 NEFFNLPLKEKMKYKSDDVCKAVRYGTSLKDGVDKIKFWRVFLKQYAHPLEDWIDSWPTN 180
            EFFNLPLKEKMKYKSDDV KAVRYGTSLKDGVDKIKFWRVFLK YAHPLEDW+DSWPTN
Sbjct: 121 REFFNLPLKEKMKYKSDDVSKAVRYGTSLKDGVDKIKFWRVFLKHYAHPLEDWVDSWPTN 180

Query: 181 PPNYREKMGQYTKEVRKVSLEVMEAITESLRLSPTYLSGKMAEGVQVVAVNCYPPCPQPG 240
           P  YREKMGQYTKEVRKVSLE+ EAITESL L PTYLS KMAEGVQVVAVNCYPPCPQPG
Sbjct: 181 PHTYREKMGQYTKEVRKVSLEIEEAITESLGLRPTYLSSKMAEGVQVVAVNCYPPCPQPG 240

Query: 241 VALGLPPHTDYSCITTVLQSSQGLEIMDPGDGSWKMVPNLDGALQVHVGDHVEVLSNGLY 300
           VALGLPPHTDYSCITT+LQSSQGLEIMD  DG WKMVPN+DG+LQVHVGDHVEVLSNGLY
Sbjct: 241 VALGLPPHTDYSCITTILQSSQGLEIMDRADGKWKMVPNVDGSLQVHVGDHVEVLSNGLY 300

Query: 301 KGVVHRATVNSERTRISITSLHSLGMDEKMKPAEELVDEQNPMKYKDSSFNDFLKFLSSN 355
           KGVVHRA VNSERTRISI+SLHSLGMDEKMKPAEELVDEQNP+KYK+SSFNDFLKFLSSN
Sbjct: 301 KGVVHRAIVNSERTRISISSLHSLGMDEKMKPAEELVDEQNPIKYKESSFNDFLKFLSSN 360

BLAST of Cla97C07G144150 vs. ExPASy TrEMBL
Match: A0A6J1JAE6 (LOW QUALITY PROTEIN: flavanone 3-dioxygenase 3 OS=Cucurbita maxima OX=3661 GN=LOC111482715 PE=3 SV=1)

HSP 1 Score: 623.2 bits (1606), Expect = 6.5e-175
Identity = 314/382 (82.20%), Postives = 334/382 (87.43%), Query Frame = 0

Query: 1   MGLKGESSGTFPTGETAQEKGLSYVPNAYVIPAPHRPSLSPQIAVVPVIDMASLRSNDSV 60
           MGL+GESSG+F TG+TAQEKGL+YVPNAYVIP P RPSLSP+ A+VP+IDMASLRSNDS 
Sbjct: 1   MGLRGESSGSFSTGQTAQEKGLNYVPNAYVIPTPQRPSLSPETAIVPIIDMASLRSNDSA 60

Query: 61  QRSLAIEELRKACISLGFF----------------------------QIINHGISETVME 120
           +RSLAIE LRKACISLGFF                            QIINHGIS+TVME
Sbjct: 61  ERSLAIEALRKACISLGFFQVKFQFFTLILETGEIYISYLLTSICFLQIINHGISKTVME 120

Query: 121 EALNQANEFFNLPLKEKMKYKSDDVCKAVRYGTSLKDGVDKIKFWRVFLKQYAHPLEDWI 180
           EAL+QA EFFNLPLKEKMKYKSDDV KAVRYGTSLKDGVDKIKFWRVFLK YAHPLEDW+
Sbjct: 121 EALSQAREFFNLPLKEKMKYKSDDVSKAVRYGTSLKDGVDKIKFWRVFLKHYAHPLEDWV 180

Query: 181 DSWPTNPPNYREKMGQYTKEVRKVSLEVMEAITESLRLSPTYLSGKMAEGVQVVAVNCYP 240
           DSWPTNP  YREKMGQYTKEVRKVSLE+ EAITESL L P YLS KMAEGVQVVAVNCYP
Sbjct: 181 DSWPTNPHTYREKMGQYTKEVRKVSLEIEEAITESLGLRP-YLSSKMAEGVQVVAVNCYP 240

Query: 241 PCPQPGVALGLPPHTDYSCITTVLQSSQGLEIMDPGDGSWKMVPNLDGALQVHVGDHVEV 300
           PCPQPGVALGLPPHTDYSCITT+LQSSQGLEIMD  DG WKMVPN+DG+LQVHVGDHVEV
Sbjct: 241 PCPQPGVALGLPPHTDYSCITTILQSSQGLEIMDRADGKWKMVPNVDGSLQVHVGDHVEV 300

Query: 301 LSNGLYKGVVHRATVNSERTRISITSLHSLGMDEKMKPAEELVDEQNPMKYKDSSFNDFL 355
           LSNGLYKGVVHRA VNSERTRISI+SLHSLGMDEKMKPAEELVDEQNP+KYK+SSFNDFL
Sbjct: 301 LSNGLYKGVVHRAIVNSERTRISISSLHSLGMDEKMKPAEELVDEQNPIKYKESSFNDFL 360

BLAST of Cla97C07G144150 vs. ExPASy TrEMBL
Match: A0A6J1CIV6 (flavanone 3-dioxygenase 3 OS=Momordica charantia OX=3673 GN=LOC111011393 PE=3 SV=1)

HSP 1 Score: 606.3 bits (1562), Expect = 8.2e-170
Identity = 305/381 (80.05%), Postives = 327/381 (85.83%), Query Frame = 0

Query: 1   MGLKGESSGTFPTGETAQEKGLSYVPNAYVIPAPHRPSLSPQIAVVPVIDMASLRSNDSV 60
           MGL GESSG+FPTGE+AQEKGLSYVPNAYVIPAPHR S S + A++P+IDMASLRS DS 
Sbjct: 1   MGLGGESSGSFPTGESAQEKGLSYVPNAYVIPAPHRSSSSRETAIIPIIDMASLRSTDSA 60

Query: 61  QRSLAIEELRKACISLGFF---------------------------QIINHGISETVMEE 120
           QRSLAIEELR+ACI LGFF                           QIINH I+E VMEE
Sbjct: 61  QRSLAIEELREACIRLGFFQVSFNASILHSDPEKICITSLTSVCFVQIINHRINERVMEE 120

Query: 121 ALNQANEFFNLPLKEKMKYKSDDVCKAVRYGTSLKDGVDKIKFWRVFLKQYAHPLEDWID 180
           AL QA EFF+LPLKEKMKYKSDDV +AVRYGTSLKDGVDKIKFWRVFLK YAHPLEDWI 
Sbjct: 121 ALEQAYEFFDLPLKEKMKYKSDDVSRAVRYGTSLKDGVDKIKFWRVFLKHYAHPLEDWIH 180

Query: 181 SWPTNPPNYREKMGQYTKEVRKVSLEVMEAITESLRLSPTYLSGKMAEGVQVVAVNCYPP 240
           SWPT+P +YREKMG YT+EVRKV LEVMEAITESL L PTYLS KMAEGVQVVAVNCYPP
Sbjct: 181 SWPTSPRSYREKMGTYTREVRKVGLEVMEAITESLGLCPTYLSSKMAEGVQVVAVNCYPP 240

Query: 241 CPQPGVALGLPPHTDYSCITTVLQSSQGLEIMDPGDGSWKMVPNLDGALQVHVGDHVEVL 300
           CPQP VALGLPPHTDYSCITT+LQSS+GLEIMD  DG+WK+VPN+DGALQVH+GDHVEVL
Sbjct: 241 CPQPEVALGLPPHTDYSCITTILQSSEGLEIMDMADGTWKLVPNIDGALQVHLGDHVEVL 300

Query: 301 SNGLYKGVVHRATVNSERTRISITSLHSLGMDEKMKPAEELVDEQNPMKYKDSSFNDFLK 355
           SNGLYKGVVHRATVNSE TRISITSLHSLGMDEKMKPAEELVD+QNP KYK+SSFNDFLK
Sbjct: 301 SNGLYKGVVHRATVNSESTRISITSLHSLGMDEKMKPAEELVDQQNPKKYKESSFNDFLK 360

BLAST of Cla97C07G144150 vs. TAIR 10
Match: AT5G07480.1 (KAR-UP oxidoreductase 1 )

HSP 1 Score: 404.1 bits (1037), Expect = 1.2e-112
Identity = 197/356 (55.34%), Postives = 254/356 (71.35%), Query Frame = 0

Query: 4   KGESSGTFPTGETAQEKGLSYVPNAYVIPAPHRPSLSPQIAVVPVIDMASLRSNDSVQRS 63
           KGE S TF  G +AQE+ L YVP+ YV+P   +P  S    +VP ID++ L+  D  +R 
Sbjct: 3   KGEDSSTFKMGNSAQERSLPYVPDCYVVPPSSKPCDS-NSGIVPTIDVSRLKGGDDERRG 62

Query: 64  LAIEELRKACISLGFFQIINHGISETVMEEALNQANEFFNLPLKEKMKYKSDDVCKAVRY 123
                            I+NHGI++ ++++AL  AN FF LP KEK ++ S+DV   VRY
Sbjct: 63  -----------------IVNHGINQNILDDALEVANSFFELPAKEKKQFMSNDVYAPVRY 122

Query: 124 GTSLKDGVDKIKFWRVFLKQYAHPLEDWIDSWPTNPPNYREKMGQYTKEVRKVSLEVMEA 183
            TSLKDG+D I+FWR+FLK YAHPL  WI  WP NPP YREKMG++ +EVRK+S+E+M A
Sbjct: 123 STSLKDGLDTIQFWRIFLKHYAHPLHRWIHLWPENPPGYREKMGKFCEEVRKLSIELMGA 182

Query: 184 ITESLRLSPTYLSGKMAE-GVQVVAVNCYPPCPQPGVALGLPPHTDYSCITTVLQSSQGL 243
           ITESL L   YLS +M E G+QV+ VNCYPPCP P  ALGLPPH+DYSCIT +LQ+  GL
Sbjct: 183 ITESLGLGRDYLSSRMDENGMQVMTVNCYPPCPDPETALGLPPHSDYSCITLLLQNLDGL 242

Query: 244 EIMDP----GDGSWKMVPNLDGALQVHVGDHVEVLSNGLYKGVVHRATVNSERTRISITS 303
           +I DP    G G W  VP + G L+VH+GDHVEVLSNGLYK +VH+ T+N E+TRIS+ S
Sbjct: 243 KIFDPMAHGGSGRWVGVPQVTGVLKVHIGDHVEVLSNGLYKSIVHKVTLNEEKTRISLAS 302

Query: 304 LHSLGMDEKMKPAEELVDEQNPMKYKDSSFNDFLKFLSSNDLAEGKSFINTLKIQD 355
           LHSLGMD+KM    ELV+++NP++YK+SSFNDFL FL  ND+++G  FI+TL+I+D
Sbjct: 303 LHSLGMDDKMSVPRELVNDENPVRYKESSFNDFLDFLVKNDISQGDRFIDTLRIKD 340

BLAST of Cla97C07G144150 vs. TAIR 10
Match: AT3G60290.1 (2-oxoglutarate (2OG) and Fe(II)-dependent oxygenase superfamily protein )

HSP 1 Score: 336.7 bits (862), Expect = 2.3e-92
Identity = 165/345 (47.83%), Postives = 232/345 (67.25%), Query Frame = 0

Query: 11  FPTGETAQEKGLSYVPNAYVIPAPHRPSLSPQIAV----VPVIDMASLRSNDSVQRSLAI 70
           F +     E G  +VP  Y++P   RP L P I      +PVID++ L  +D + R   I
Sbjct: 13  FTSAMELTESGDPHVPTRYILPPSQRPMLGPSIGTSTINLPVIDLSFL--HDPLLRLCVI 72

Query: 71  EELRKACISLGFFQIINHGISETVMEEALNQANEFFNLPLKEKMKYKSDDVCKAVRYGTS 130
            E+  AC   GFFQ+INHGIS  V+++A + A  FF+LP  EKM   SD+  + VRYGTS
Sbjct: 73  HEIELACKGFGFFQVINHGISSAVVKDAQDSATRFFDLPADEKMHLVSDNFQEPVRYGTS 132

Query: 131 LKDGVDKIKFWRVFLKQYAHPLEDWIDSWPTNPPNYREKMGQYTKEVRKVSLEVMEAITE 190
           +    D++ +WR F+K Y+HPL +WI+ WP+NPP Y+EK+G+Y +    +  +++EAI+E
Sbjct: 133 INHSTDRVHYWRDFIKHYSHPLSNWINLWPSNPPCYKEKVGKYAEATHVLHKQLIEAISE 192

Query: 191 SLRLSPTYLSGKMAEGVQVVAVNCYPPCPQPGVALGLPPHTDYSCITTVLQSSQGLEIMD 250
           SL L   YL  ++ EG QV+AVNCYP CP+P +ALG+PPH+DY  +T +LQSS+GL+I D
Sbjct: 193 SLGLEKNYLQEEIEEGSQVMAVNCYPACPEPEIALGMPPHSDYGSLTILLQSSEGLQIKD 252

Query: 251 PGDGSWKMVPNLDGALQVHVGDHVEVLSNGLYKGVVHRATVNSERTRISITSLHSLGMDE 310
             + +W  VP ++GAL V +GD VEV+SNG+YK VVHR TVN +  R+S  SLHSL M +
Sbjct: 253 -CNNNWVCVPYIEGALIVQLGDQVEVMSNGIYKSVVHRVTVNKDYKRLSFASLHSLPMHK 312

Query: 311 KMKPAEELVDEQNPMKYKDSSFNDFLKFLSSNDLAEGKSFINTLK 352
           K+ PA +LV+E  P  Y + SFNDFL ++S ND+ + K FI+TL+
Sbjct: 313 KISPATQLVNENKPAAYGEFSFNDFLDYISRNDITQ-KRFIDTLR 353

BLAST of Cla97C07G144150 vs. TAIR 10
Match: AT2G44800.1 (2-oxoglutarate (2OG) and Fe(II)-dependent oxygenase superfamily protein )

HSP 1 Score: 336.3 bits (861), Expect = 3.0e-92
Identity = 166/346 (47.98%), Postives = 238/346 (68.79%), Query Frame = 0

Query: 10  TFPTGETAQEKGLSYVPNAYVIPAPHRPSLSPQI----AVVPVIDMASLRSNDSVQRSLA 69
           +F +  T    G+  VP+ YV+P   RP+L   +      +PVID++ L  +    RSLA
Sbjct: 12  SFTSAMTLTNSGVPQVPDRYVLPPSQRPALGSSLGTSETTLPVIDLSLL--HQPFLRSLA 71

Query: 70  IEELRKACISLGFFQIINHGISETVMEEALNQANEFFNLPLKEKMKYKSDDVCKAVRYGT 129
           I E+  AC   GFFQ+INHGI  +V+ +AL+ A +FF+LP++EKM   S +V + VRYGT
Sbjct: 72  IHEISMACKEFGFFQVINHGIPSSVVNDALDAATQFFDLPVEEKMLLVSANVHEPVRYGT 131

Query: 130 SLKDGVDKIKFWRVFLKQYAHPLEDWIDSWPTNPPNYREKMGQYTKEVRKVSLEVMEAIT 189
           SL    D++ +WR F+K Y+HPL  WID WP+NPP Y++K+G+Y +    +  +++EAI+
Sbjct: 132 SLNHSTDRVHYWRDFIKHYSHPLSKWIDMWPSNPPCYKDKVGKYAEATHLLHKQLIEAIS 191

Query: 190 ESLRLSPTYLSGKMAEGVQVVAVNCYPPCPQPGVALGLPPHTDYSCITTVLQSSQGLEIM 249
           ESL L   YL  ++ EG QV+AVNCYP CP+P +ALG+PPH+D+S +T +LQSS+GL+IM
Sbjct: 192 ESLGLEKNYLQEEIEEGSQVMAVNCYPACPEPEMALGMPPHSDFSSLTILLQSSKGLQIM 251

Query: 250 DPGDGSWKMVPNLDGALQVHVGDHVEVLSNGLYKGVVHRATVNSERTRISITSLHSLGMD 309
           D  + +W  VP ++GAL V +GD VEV+SNG+YK V+HR TVN E  R+S  SLHSL + 
Sbjct: 252 D-CNKNWVCVPYIEGALIVQLGDQVEVMSNGIYKSVIHRVTVNKEVKRLSFASLHSLPLH 311

Query: 310 EKMKPAEELVDEQNPMKYKDSSFNDFLKFLSSNDLAEGKSFINTLK 352
           +K+ PA +LV+  N   Y + SFNDFL ++SSND  + + FI+T+K
Sbjct: 312 KKISPAPKLVNPNNAPAYGEFSFNDFLNYISSNDFIQ-ERFIDTIK 353

BLAST of Cla97C07G144150 vs. TAIR 10
Match: AT2G36690.1 (2-oxoglutarate (2OG) and Fe(II)-dependent oxygenase superfamily protein )

HSP 1 Score: 267.7 bits (683), Expect = 1.3e-71
Identity = 138/344 (40.12%), Postives = 209/344 (60.76%), Query Frame = 0

Query: 19  EKGLSYVPNAYVIPAPHRPSLSPQIAVV--------PVIDMASLRSNDSVQRSLAIEELR 78
           E GL+ VP  Y+ P P RP L+    ++        P+ID A L   +   R   +  + 
Sbjct: 26  ENGLTKVPTKYIWPEPDRPILTKSDKLIKPNKNLKLPLIDFAELLGPN---RPHVLRTIA 85

Query: 79  KACISLGFFQIINHGISETVMEEALNQANEFFNLPLKEKMKYKSDDVCKAVRYGTSLKDG 138
           +AC + GFFQ++NHG+   V +  ++    FF LP +E+ KY S D+   VRYGTS    
Sbjct: 86  EACKTYGFFQVVNHGMEGDVSKNMIDVCKRFFELPYEERSKYMSSDMSAPVRYGTSFNQI 145

Query: 139 VDKIKFWRVFLKQYAHPLEDWIDSWPTNPPNYREKMGQYTKEVRKVSLEVMEAITESLRL 198
            D +  WR FLK YAHPL D++  WP++P ++R     Y KE +++   +++AI ESL +
Sbjct: 146 KDNVFCWRDFLKLYAHPLPDYLPHWPSSPSDFRSSAATYAKETKEMFEMMVKAILESLEI 205

Query: 199 SPTYLSGK-MAEGVQVVAVNCYPPCPQPGVALGLPPHTDYSCITTVLQSS-QGLEIMDPG 258
             +  + K + EG QVV VNCYPPCP+P + LG+PPH+DY  +T +LQ   +GL+I+   
Sbjct: 206 DGSDEAAKELEEGSQVVVVNCYPPCPEPELTLGMPPHSDYGFLTLLLQDEVEGLQILYRD 265

Query: 259 DGSWKMVPNLDGALQVHVGDHVEVLSNGLYKGVVHRATVNSERTRISITSLHSLGMDEKM 318
           +  W  V  + G+  V+VGDH+E+ SNG YK V+HR  VNS + RIS+ SLHS  +   +
Sbjct: 266 E--WVTVDPIPGSFVVNVGDHLEIFSNGRYKSVLHRVLVNSTKPRISVASLHSFPLTSVV 325

Query: 319 KPAEELVDEQNPMKYKDSSFNDFLKFLSSNDLAEGKSFINTLKI 353
           KP+ +LVD+ NP +Y D+ F  FL++++S +  + K+F+ + KI
Sbjct: 326 KPSPKLVDKHNPSQYMDTDFTTFLQYITSRE-PKWKNFLESRKI 363

BLAST of Cla97C07G144150 vs. TAIR 10
Match: AT5G24530.1 (2-oxoglutarate (2OG) and Fe(II)-dependent oxygenase superfamily protein )

HSP 1 Score: 228.4 bits (581), Expect = 9.0e-60
Identity = 121/333 (36.34%), Postives = 195/333 (58.56%), Query Frame = 0

Query: 25  VPNAYVIPAPHRPSLS--PQIAVVPVIDMASLRSNDSVQRSLAIEELRKACISLGFFQII 84
           +P  YV P   RP LS   Q+   P+ID++      S  RS  I+++ +AC   GFFQ+I
Sbjct: 15  LPENYVRPISDRPRLSEVSQLEDFPLIDLS------STDRSFLIQQIHQACARFGFFQVI 74

Query: 85  NHGISETVMEEALNQANEFFNLPLKEKMKYKSDDVCKAVRYGTSLKDGVDKIKFWRVFLK 144
           NHG+++ +++E ++ A EFF++ ++EKMK  SDD  K  R  TS     +++  WR +L+
Sbjct: 75  NHGVNKQIIDEMVSVAREFFSMSMEEKMKLYSDDPTKTTRLSTSFNVKKEEVNNWRDYLR 134

Query: 145 QYAHPLEDWIDSWPTNPPNYREKMGQYTKEVRKVSLEVMEAITESLRLSPTYLSGKMAEG 204
            + +P+  +++ WP+NPP+++E + +Y++EVR+V  ++ E I+ESL L   Y+   + E 
Sbjct: 135 LHCYPIHKYVNEWPSNPPSFKEIVSKYSREVREVGFKIEELISESLGLEKDYMKKVLGEQ 194

Query: 205 VQVVAVNCYPPCPQPGVALGLPPHTDYSCITTVLQSSQ--GLEIMDPGDGSWKMVPNLDG 264
            Q +AVN YPPCP+P +  GLP HTD + +T +LQ +   GL+I+   DG W  V     
Sbjct: 195 GQHMAVNYYPPCPEPELTYGLPAHTDPNALTILLQDTTVCGLQILI--DGQWFAVNPHPD 254

Query: 265 ALQVHVGDHVEVLSNGLYKGVVHRATVNSERTRISITSL---HSLGMDEKMKPAEELVDE 324
           A  +++GD ++ LSNG+YK V HRA  N+E  R+S+ S        +    KP  E  D+
Sbjct: 255 AFVINIGDQLQALSNGVYKSVWHRAVTNTENPRLSVASFLCPADCAVMSPAKPLWEAEDD 314

Query: 325 QNPMKYKDSSFNDFLKFLSSNDLAEGKSFINTL 351
           +    YKD ++ ++ K   S +L +     N L
Sbjct: 315 ETKPVYKDFTYAEYYKKFWSRNLDQEHCLENFL 339

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
XP_004146935.28.6e-19092.37flavanone 3-dioxygenase 3 [Cucumis sativus] >KGN53184.2 hypothetical protein Csa... [more]
XP_008453850.11.9e-18992.66PREDICTED: protein DOWNY MILDEW RESISTANCE 6 [Cucumis melo] >KAA0044683.1 protei... [more]
XP_038889497.11.2e-18390.50flavanone 3-dioxygenase 3 [Benincasa hispida][more]
XP_022922584.11.2e-17884.57LOW QUALITY PROTEIN: flavanone 3-dioxygenase 3 [Cucurbita moschata][more]
XP_023552054.12.0e-17883.64LOW QUALITY PROTEIN: flavanone 3-dioxygenase 3 [Cucurbita pepo subsp. pepo][more]
Match NameE-valueIdentityDescription
Q7XR841.5e-8348.14Flavanone 3-dioxygenase 3 OS=Oryza sativa subsp. japonica OX=39947 GN=F3H-3 PE=1... [more]
Q7XR837.6e-8048.902-oxoglutarate-dependent dioxygenase 21, chloroplastic OS=Oryza sativa subsp. ja... [more]
Q6YYX92.5e-6739.77Probable 2-oxoglutarate-dependent dioxygenase SLC1 OS=Oryza sativa subsp. japoni... [more]
Q651B62.1e-6140.17Probable 2-oxoglutarate-dependent dioxygenase SLC2 OS=Oryza sativa subsp. japoni... [more]
Q9FLV01.3e-5836.34Protein DOWNY MILDEW RESISTANCE 6 OS=Arabidopsis thaliana OX=3702 GN=DMR6 PE=1 S... [more]
Match NameE-valueIdentityDescription
A0A5A7TMH39.3e-19092.66Protein DOWNY MILDEW RESISTANCE 6 OS=Cucumis melo var. makuwa OX=1194695 GN=E567... [more]
A0A1S3BX869.3e-19092.66protein DOWNY MILDEW RESISTANCE 6 OS=Cucumis melo OX=3656 GN=LOC103494453 PE=3 S... [more]
A0A6J1E3T85.7e-17984.57LOW QUALITY PROTEIN: flavanone 3-dioxygenase 3 OS=Cucurbita moschata OX=3662 GN=... [more]
A0A6J1JAE66.5e-17582.20LOW QUALITY PROTEIN: flavanone 3-dioxygenase 3 OS=Cucurbita maxima OX=3661 GN=LO... [more]
A0A6J1CIV68.2e-17080.05flavanone 3-dioxygenase 3 OS=Momordica charantia OX=3673 GN=LOC111011393 PE=3 SV... [more]
Match NameE-valueIdentityDescription
AT5G07480.11.2e-11255.34KAR-UP oxidoreductase 1 [more]
AT3G60290.12.3e-9247.832-oxoglutarate (2OG) and Fe(II)-dependent oxygenase superfamily protein [more]
AT2G44800.13.0e-9247.982-oxoglutarate (2OG) and Fe(II)-dependent oxygenase superfamily protein [more]
AT2G36690.11.3e-7140.122-oxoglutarate (2OG) and Fe(II)-dependent oxygenase superfamily protein [more]
AT5G24530.19.0e-6036.342-oxoglutarate (2OG) and Fe(II)-dependent oxygenase superfamily protein [more]
InterPro
Analysis Name: InterPro Annotations of Watermelon (97103) v2.5
Date Performed: 2022-01-31
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR027443Isopenicillin N synthase-like superfamilyGENE3D2.60.120.330coord: 15..339
e-value: 4.9E-86
score: 291.0
IPR026992Non-haem dioxygenase N-terminal domainPFAMPF14226DIOX_Ncoord: 46..156
e-value: 4.9E-24
score: 85.3
IPR044861Isopenicillin N synthase-like, Fe(2+) 2OG dioxygenase domainPFAMPF031712OG-FeII_Oxycoord: 206..300
e-value: 7.4E-21
score: 74.5
NoneNo IPR availablePANTHERPTHR47991OXOGLUTARATE/IRON-DEPENDENT DIOXYGENASEcoord: 10..339
NoneNo IPR availablePANTHERPTHR47991:SF67KAR-UP OXIDOREDUCTASE 1coord: 10..339
NoneNo IPR availableSUPERFAMILY51197Clavaminate synthase-likecoord: 17..337
IPR005123Oxoglutarate/iron-dependent dioxygenasePROSITEPS51471FE2OG_OXYcoord: 199..302
score: 10.795691

Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
Cla97C07G144150.1Cla97C07G144150.1mRNA


GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
molecular_function GO:0051213 dioxygenase activity
molecular_function GO:0046872 metal ion binding