Cla97C07G132015 (gene) Watermelon (97103) v2.5

Overview


Name	Cla97C07G132015
Type	gene
Organism	Citrullus lanatus subsp. vulgaris cv. 97103 (Watermelon (97103) v2.5)
Description	Cucumisin
Location	Cla97Chr07: 3752946 .. 3754237 (+)
RNA-Seq Expression	Cla97C07G132015
Synteny	Cla97C07G132015

Sequences

The following sequences are available for this feature:

Gene sequence (with intron)

Legend: exonCDSpolypeptide

Hold the cursor over a type above to highlight its positions in the sequence below.

ATGTCCATACCCATTTATGATTACATTGACTCAGCTCGGTATGTTTCTCAATATAAATAGCCTTTCAATGCCACTTTTTTCCCCTAAATTGTTTGAGACAACTCAAGAATGTGAATAGAAACAACTATTATTAAAGAAAGTTGCATATTCCCAGGTTCCATGGAGACACCTTACACTCTTTATACGATATATAAGTTACTCTTCTCATTGGAGACACTCTACACTCTTTATTTCTCATACTAAATTAGTTTAAGATGGAACCTAATGTTTCTCTAATATGGTATTAAAGCCCATAAAACTCAAATGAGTACCTGATCTAAAAAGAATAATCAAATCATGCAGGAATGTTGAACCTGAAAAATACAATGTTGAGAATATCCCACATTGAAAAAGTGAAAGAACCTTATAGTTATTGATTTTGAGATGGAATCCCATTTCATCTAATAGCGTATTTATTGAATTATTTTATCAATATTGAGAGGTTCATTTATTTATTTATTTATTTTTATCAACTTTTGATGAATAGCTCTCCTACTGCAACCATTTTCAAGAGTACCACACTGCATAATGTGTCTGCTCCTGTTGTAATTTCCTTCTCATCCAGGGGACCTAATACTCTAACCAACGACATTCTCAAGGTTAAATTTATTTGAACTTTGCCTTTCTATATTGTTAGGTTAGGTAAGATGATTAATAAACTAATGTTATGAACTTGCACAAATGCAGCCAGATTTGAGTGGTCCAGGAGTTGAAATTTTAGCAGCATGGCCTCCTGTTGCGCCTGTTGCTAAAGTTCAAGATTCCAGGAGTACGCTTTGTAATATAATCTCAAGAATGTCAATGTCTAGCCCACATATCACTGGAATTGCAGTGTATGTTAAAACATTCAATCCTACGTGGTCTACTGCTGCCATCAAGTCAGCACTCATGACAACTGGTAATTATCACAGCCATGATATGACTAATTAACAAAGATTGTGGACTGCTCATCTTTCTAATATCCTTCTTTTGGACTTTCTATATCTCGTTCGCAAACCTTCAATCAATACTTCACAAGAACTCTCACAAGTGTTGCATCTTCAGCATCCACATATACAGCTATAATTTCTGCCCCACAAGGCTTTGAGATCACAGTGGATCCTAATATTCTATCATTCAATGGCATTGGAGATAGGAAATCTTTCACGTTGACTGTTCGAGGATCAGTGAGCCAGTTCATAGTCTCTGCTTCTTTGGTGTGGAGCGATGGTGTGCACACTGTGAGAAGCCCTATAACAATCACTTCTCGCTAG

mRNA sequence

ATGTCCATACCCATTTATGATTACATTGACTCAGCTCGCTCTCCTACTGCAACCATTTTCAAGAGTACCACACTGCATAATGTGTCTGCTCCTGTTGTAATTTCCTTCTCATCCAGGGGACCTAATACTCTAACCAACGACATTCTCAAGCCAGATTTGAGTGGTCCAGGAGTTGAAATTTTAGCAGCATGGCCTCCTGTTGCGCCTGTTGCTAAAGTTCAAGATTCCAGGAGTACGCTTTGTAATATAATCTCAAGAATGTCAATGTCTAGCCCACATATCACTGGAATTGCAGTGTATGTTAAAACATTCAATCCTACGTGGTCTACTGCTGCCATCAAGTCAGCACTCATGACAACTGCATCCACATATACAGCTATAATTTCTGCCCCACAAGGCTTTGAGATCACAGTGGATCCTAATATTCTATCATTCAATGGCATTGGAGATAGGAAATCTTTCACGTTGACTGTTCGAGGATCAGTGAGCCAGTTCATAGTCTCTGCTTCTTTGGTGTGGAGCGATGGTGTGCACACTGTGAGAAGCCCTATAACAATCACTTCTCGCTAG

Coding sequence (CDS)

ATGTCCATACCCATTTATGATTACATTGACTCAGCTCGCTCTCCTACTGCAACCATTTTCAAGAGTACCACACTGCATAATGTGTCTGCTCCTGTTGTAATTTCCTTCTCATCCAGGGGACCTAATACTCTAACCAACGACATTCTCAAGCCAGATTTGAGTGGTCCAGGAGTTGAAATTTTAGCAGCATGGCCTCCTGTTGCGCCTGTTGCTAAAGTTCAAGATTCCAGGAGTACGCTTTGTAATATAATCTCAAGAATGTCAATGTCTAGCCCACATATCACTGGAATTGCAGTGTATGTTAAAACATTCAATCCTACGTGGTCTACTGCTGCCATCAAGTCAGCACTCATGACAACTGCATCCACATATACAGCTATAATTTCTGCCCCACAAGGCTTTGAGATCACAGTGGATCCTAATATTCTATCATTCAATGGCATTGGAGATAGGAAATCTTTCACGTTGACTGTTCGAGGATCAGTGAGCCAGTTCATAGTCTCTGCTTCTTTGGTGTGGAGCGATGGTGTGCACACTGTGAGAAGCCCTATAACAATCACTTCTCGCTAG

Protein sequence

MSIPIYDYIDSARSPTATIFKSTTLHNVSAPVVISFSSRGPNTLTNDILKPDLSGPGVEILAAWPPVAPVAKVQDSRSTLCNIISRMSMSSPHITGIAVYVKTFNPTWSTAAIKSALMTTASTYTAIISAPQGFEITVDPNILSFNGIGDRKSFTLTVRGSVSQFIVSASLVWSDGVHTVRSPITITSR

Homology

[Close]

BLAST of Cla97C07G132015 vs. NCBI nr
Match: KAA0051619.1 (cucumisin [Cucumis melo var. makuwa])

HSP 1 Score: 227.3 bits (578), Expect = 1.1e-55
Identity = 140/286 (48.95%), Postives = 158/286 (55.24%), Query Frame = 0


Query: 8   YIDSARSPTATIFKSTTLHNVSAPVVISFSSRGPNTLTNDILKPDLSGPGVEILAAWPPV 67
           YI S RSP ATIFKSTT+ N SAPVV+SFSSRGPN  T D++KPD+SGPGVEILAAWP V
Sbjct: 398 YIYSIRSPGATIFKSTTILNASAPVVVSFSSRGPNRATKDVIKPDISGPGVEILAAWPSV 457

Query: 68  APVAKVQDSRSTLCNIISRMSMSSPHITGIAVYVKTFNPTWSTAAIKSALMTT------- 127
           APV  ++  R+TL NIIS  SMS PHITGIA YVKT+NPTWS AAIKSALMTT       
Sbjct: 458 APVGGIR--RNTLFNIISGTSMSCPHITGIATYVKTYNPTWSPAAIKSALMTTASPMNAR 517

Query: 128 ------------------------------------------------------------ 187
                                                                       
Sbjct: 518 FNPQAEFAYGSGHVNPLKAVRPGLVYDANESDYVKFLCGQGYNTEAVRRITGDYSACTPG 577

Query: 188 -------------------------------------ASTYTAIISAPQGFEITVDPNIL 190
                                                ASTY A+ISAPQG  I+V+PN+L
Sbjct: 578 NTGRVWDLNYPSFGLSVSPSKTFNQYFNRTLTSVAPQASTYRAMISAPQGLTISVNPNVL 637

[Close]

BLAST of Cla97C07G132015 vs. NCBI nr
Match: XP_038892506.1 (cucumisin-like [Benincasa hispida])

HSP 1 Score: 226.9 bits (577), Expect = 1.5e-55
Identity = 139/288 (48.26%), Postives = 157/288 (54.51%), Query Frame = 0


Query: 5   IYDYIDSARSPTATIFKSTTLHNVSAPVVISFSSRGPNTLTNDILKPDLSGPGVEILAAW 64
           I+ YI S   PTATIFKST + N SAPVV+SFSSRGPN  T D++KPDLS PGVEILAAW
Sbjct: 450 IFRYIYSTSFPTATIFKSTAILNTSAPVVVSFSSRGPNAATKDLIKPDLSAPGVEILAAW 509

Query: 65  PPVAPVAKVQDSRSTLCNIISRMSMSSPHITGIAVYVKTFNPTWSTAAIKSALMTT---- 124
           PPVAPV  +   R TL NIIS  SMS PH+TGIA YVKTFNPTWS AAIKSALMTT    
Sbjct: 510 PPVAPVGGI--LRDTLYNIISGTSMSCPHVTGIAAYVKTFNPTWSPAAIKSALMTTASPM 569

Query: 125 ------------------------------------------------------------ 184
                                                                       
Sbjct: 570 NARFHPQGEFAYGSGHVNPLKAVRPGLVYDANESDYVKFLCGQGYSTSMVRRITGDYSAC 629

Query: 185 ----------------------------------------ASTYTAIISAPQGFEITVDP 189
                                                   ASTY A+ISAPQG  ITV+P
Sbjct: 630 TSGNIGRVWDLNYPSFGLSVSGSQTFSQYFTRTLTSVASQASTYRAMISAPQGLAITVNP 689

[Close]

BLAST of Cla97C07G132015 vs. NCBI nr
Match: XP_008461721.1 (PREDICTED: LOW QUALITY PROTEIN: cucumisin [Cucumis melo])

HSP 1 Score: 226.1 bits (575), Expect = 2.5e-55
Identity = 140/285 (49.12%), Postives = 157/285 (55.09%), Query Frame = 0


Query: 8   YIDSARSPTATIFKSTTLHNVSAPVVISFSSRGPNTLTNDILKPDLSGPGVEILAAWPPV 67
           YI S RSP ATIFKSTT+ N SAPVV+SFSSRGPN  T D++KPD+SGPGVEILAAWP V
Sbjct: 447 YIYSIRSPGATIFKSTTILNASAPVVVSFSSRGPNRATKDVIKPDISGPGVEILAAWPSV 506

Query: 68  APVAKVQDSRSTLCNIISRMSMSSPHITGIAVYVKTFNPTWSTAAIKSALMTT------- 127
           APV  ++  R+TL NIIS  SMS PHITGIA YVKT+NPTWS AAIKSALMTT       
Sbjct: 507 APVGGIR--RNTLFNIISGTSMSCPHITGIATYVKTYNPTWSPAAIKSALMTTASPMNAR 566

Query: 128 ------------------------------------------------------------ 187
                                                                       
Sbjct: 567 FNPQAEFAYGSGHVNPLKAVRPGLVYDANESDYVKFLCGQGYNTEAVRRITGDYSACTPG 626

Query: 188 -------------------------------------ASTYTAIISAPQGFEITVDPNIL 189
                                                ASTY A+ISAPQG  I+V+PN+L
Sbjct: 627 NTGRVWDLNYPSFGLSVSPSKTFNQYFNRTLTSVAPQASTYRAMISAPQGLTISVNPNVL 686

[Close]

BLAST of Cla97C07G132015 vs. NCBI nr
Match: TYK30793.1 (cucumisin [Cucumis melo var. makuwa])

HSP 1 Score: 224.9 bits (572), Expect = 5.7e-55
Identity = 140/292 (47.95%), Postives = 158/292 (54.11%), Query Frame = 0


Query: 8   YIDSARSPTATIFKSTTLHNVSAPVVISFSSRGPNTLTNDILKPDLSGPGVEILAAWPPV 67
           YI S RSP ATIFKSTT+ N SAPVV+SFSSRGPN  T D++KPD+SGPGVEILAAWP V
Sbjct: 394 YIYSIRSPGATIFKSTTILNASAPVVVSFSSRGPNRATKDVIKPDISGPGVEILAAWPSV 453

Query: 68  APVAKVQDSRSTLCNIISRMSMSSPHITGIAVYVKTFNPTWSTAAIKSALMTT------- 127
           APV  ++  R+TL NIIS  SMS PHITGIA YVKT+NPTWS AAIKSALMTT       
Sbjct: 454 APVGGIR--RNTLFNIISGTSMSCPHITGIATYVKTYNPTWSPAAIKSALMTTANYFTLT 513

Query: 128 ------------------------------------------------------------ 187
                                                                       
Sbjct: 514 SPMNARFNPQAEFAYGSGHVNPLKAVRPGLVYDANESDYVKFLCGQGYNTEAVRRITGDY 573

Query: 188 -------------------------------------------ASTYTAIISAPQGFEIT 190
                                                      ASTY A+ISAPQG  I+
Sbjct: 574 SACTPGNTGRVWDLNYPSFGLSVSPSKTFNQYFNRTLTSVAPQASTYRAMISAPQGLTIS 633

[Close]

BLAST of Cla97C07G132015 vs. NCBI nr
Match: 3VTA_A (Crystal Structure of cucumisin, a subtilisin-like endoprotease from Cucumis melo L [Cucumis melo] >3VTA_B Crystal Structure of cucumisin, a subtilisin-like endoprotease from Cucumis melo L [Cucumis melo] >4YN3_A Crystal structure of Cucumisin complex with pro-peptide [Cucumis melo])

HSP 1 Score: 224.9 bits (572), Expect = 5.7e-55
Identity = 140/285 (49.12%), Postives = 156/285 (54.74%), Query Frame = 0


Query: 8   YIDSARSPTATIFKSTTLHNVSAPVVISFSSRGPNTLTNDILKPDLSGPGVEILAAWPPV 67
           YI S RSP ATIFKSTT+ N SAPVV+SFSSRGPN  T D++KPD+SGPGVEILAAWP V
Sbjct: 337 YIYSIRSPGATIFKSTTILNASAPVVVSFSSRGPNRATKDVIKPDISGPGVEILAAWPSV 396

Query: 68  APVAKVQDSRSTLCNIISRMSMSSPHITGIAVYVKTFNPTWSTAAIKSALMTT------- 127
           APV  ++  R+TL NIIS  SMS PHITGIA YVKT+NPTWS AAIKSALMTT       
Sbjct: 397 APVGGIR--RNTLFNIISGTSMSCPHITGIATYVKTYNPTWSPAAIKSALMTTASPMNAR 456

Query: 128 ------------------------------------------------------------ 187
                                                                       
Sbjct: 457 FNPQAEFAYGSGHVNPLKAVRPGLVYDANESDYVKFLCGQGYNTQAVRRITGDYSACTSG 516

Query: 188 -------------------------------------ASTYTAIISAPQGFEITVDPNIL 189
                                                ASTY A+ISAPQG  I+V+PN+L
Sbjct: 517 NTGRVWDLNYPSFGLSVSPSQTFNQYFNRTLTSVAPQASTYRAMISAPQGLTISVNPNVL 576

[Close]

BLAST of Cla97C07G132015 vs. ExPASy Swiss-Prot
Match: Q39547 (Cucumisin OS=Cucumis melo OX=3656 PE=1 SV=1)

HSP 1 Score: 224.9 bits (572), Expect = 7.4e-58
Identity = 140/285 (49.12%), Postives = 156/285 (54.74%), Query Frame = 0


Query: 8   YIDSARSPTATIFKSTTLHNVSAPVVISFSSRGPNTLTNDILKPDLSGPGVEILAAWPPV 67
           YI S RSP ATIFKSTT+ N SAPVV+SFSSRGPN  T D++KPD+SGPGVEILAAWP V
Sbjct: 447 YIYSIRSPGATIFKSTTILNASAPVVVSFSSRGPNRATKDVIKPDISGPGVEILAAWPSV 506

Query: 68  APVAKVQDSRSTLCNIISRMSMSSPHITGIAVYVKTFNPTWSTAAIKSALMTT------- 127
           APV  ++  R+TL NIIS  SMS PHITGIA YVKT+NPTWS AAIKSALMTT       
Sbjct: 507 APVGGIR--RNTLFNIISGTSMSCPHITGIATYVKTYNPTWSPAAIKSALMTTASPMNAR 566

Query: 128 ------------------------------------------------------------ 187
                                                                       
Sbjct: 567 FNPQAEFAYGSGHVNPLKAVRPGLVYDANESDYVKFLCGQGYNTQAVRRITGDYSACTSG 626

Query: 188 -------------------------------------ASTYTAIISAPQGFEITVDPNIL 189
                                                ASTY A+ISAPQG  I+V+PN+L
Sbjct: 627 NTGRVWDLNYPSFGLSVSPSQTFNQYFNRTLTSVAPQASTYRAMISAPQGLTISVNPNVL 686

[Close]

BLAST of Cla97C07G132015 vs. ExPASy Swiss-Prot
Match: Q9FIG2 (Subtilisin-like protease SBT4.13 OS=Arabidopsis thaliana OX=3702 GN=SBT4.13 PE=2 SV=1)

HSP 1 Score: 129.4 bits (324), Expect = 4.3e-29
Identity = 90/293 (30.72%), Postives = 130/293 (44.37%), Query Frame = 0


Query: 5   IYDYIDSARSPTATIFKSTTLHNVSAPVVISFSSRGPNTLTNDILKPDLSGPGVEILAAW 64
           +  Y++S  SP A + K+  + N ++PV+ SFSSRGPNT+  DILKPD++ PGVEILAA+
Sbjct: 436 LVSYLESTDSPQAIVLKTEAIFNRTSPVIASFSSRGPNTIAVDILKPDITAPGVEILAAY 495

Query: 65  PPVAPVAKVQDSRSTLCNIISRMSMSSPHITGIAVYVKTFNPTWSTAAIKSALMTTA--- 124
            P    ++  D+R    +++S  SMS PH+ G+A YVKTFNP WS + I+SA+MTTA   
Sbjct: 496 SPAGEPSQ-DDTRHVKYSVLSGTSMSCPHVAGVAAYVKTFNPKWSPSMIQSAIMTTAWPV 555

Query: 125 ------------------------------------------------------------ 184
                                                                       
Sbjct: 556 NATGTGIASTEFAYGSGHVDPIAASNPGLVYELDKSDHIAFLCGMNYTSQVLKVISGETV 615

Query: 185 --------------------------------------------STYTAIISAPQG--FE 187
                                                       STYT+ + A  G   +
Sbjct: 616 TCSEAKKILPRNLNYPSMSAKLSGSGTTFTVTFNRTLTNVGTPNSTYTSKVVAGHGSKLD 675

[Close]

BLAST of Cla97C07G132015 vs. ExPASy Swiss-Prot
Match: Q9STF7 (Subtilisin-like protease SBT4.6 OS=Arabidopsis thaliana OX=3702 GN=SBT4.6 PE=3 SV=1)

HSP 1 Score: 127.5 bits (319), Expect = 1.6e-28
Identity = 90/291 (30.93%), Postives = 130/291 (44.67%), Query Frame = 0


Query: 5   IYDYIDSARSPTATIFKSTTLHNVSAPVVISFSSRGPNTLTNDILKPDLSGPGVEILAAW 64
           +  Y++S ++P A + KS T+ N  APVV S+SSRGPN L +DILKPD++ PG EILAA+
Sbjct: 445 VLSYVNSTKNPKAAVLKSETIFNQKAPVVASYSSRGPNPLIHDILKPDITAPGSEILAAY 504

Query: 65  PPVAPVAKVQDSRSTLCNIISRMSMSSPHITGIAVYVKTFNPTWSTAAIKSALMTTA--- 124
            P  P ++  D+R     +IS  SMS PH+ G+A Y+KTF+P WS + I+SA+MTTA   
Sbjct: 505 SPYVPPSE-SDTRHVKYTVISGTSMSCPHVAGVAAYIKTFHPLWSPSMIQSAIMTTAWPM 564

Query: 125 ------------------------------------------------------------ 184
                                                                       
Sbjct: 565 NASTSPSNELAEFAYGAGHVDPIAAIHPGLVYEANKSDHITFLCGFNYTGKKLRLISGDS 624

Query: 185 -----------------STYTAIISAPQGFEIT--------------------------- 187
                             + +A +S  + F++T                           
Sbjct: 625 SSCTKEQTKSLTRNLNYPSMSAQVSGTKPFKVTFRRTVTNVGRPNATYKAKVVGSKLKVK 684

[Close]

BLAST of Cla97C07G132015 vs. ExPASy Swiss-Prot
Match: Q9FIM5 (Subtilisin-like protease SBT4.9 OS=Arabidopsis thaliana OX=3702 GN=SBT4.9 PE=2 SV=1)

HSP 1 Score: 123.6 bits (309), Expect = 2.3e-27
Identity = 86/292 (29.45%), Postives = 128/292 (43.84%), Query Frame = 0


Query: 5   IYDYIDSARSPTATIFKSTTLHNVSAPVVISFSSRGPNTLTNDILKPDLSGPGVEILAAW 64
           +  Y++S +SP  T+ KS  + N +AP V  FSSRGPNT+  DILKPD++ PGVEILAA+
Sbjct: 415 VISYVNSTKSPHGTVLKSEAIFNQAAPKVAGFSSRGPNTIAVDILKPDVTAPGVEILAAF 474

Query: 65  PPV-APVAKVQDSRSTLCNIISRMSMSSPHITGIAVYVKTFNPTWSTAAIKSALMTTA-- 124
            P+ +P    +D+R    +++S  SMS PH+ G+A Y+KTF+P WS + I+SA+MTTA  
Sbjct: 475 SPLNSPAQDKRDNRHVKYSVLSGTSMSCPHVAGVAAYIKTFHPEWSPSMIQSAIMTTAWP 534

Query: 125 ------------------------------------------------------------ 184
                                                                       
Sbjct: 535 MNATGTAVASTEFAYGAGHVDPIAAINPGLVYEIGKSDHIAFLCGLNYNATSLKLIAGEA 594

Query: 185 -------------------------------------------STYTA--IISAPQGFEI 187
                                                      STY +  +++     ++
Sbjct: 595 VTCTGKTLPRNLNYPSMSAKLPKSESSFIVTFNRTVTNVGTPNSTYKSKIVLNHGSNLKV 654

[Close]

BLAST of Cla97C07G132015 vs. ExPASy Swiss-Prot
Match: Q9FIM6 (Subtilisin-like protease SBT4.8 OS=Arabidopsis thaliana OX=3702 GN=SBT4.8 PE=3 SV=1)

HSP 1 Score: 122.5 bits (306), Expect = 5.2e-27
Identity = 89/292 (30.48%), Postives = 126/292 (43.15%), Query Frame = 0


Query: 5   IYDYIDSARSPTATIFKSTTLHNVSAPVVISFSSRGPNTLTNDILKPDLSGPGVEILAAW 64
           +  YI+S RSP  ++ K+  + N  +P V SFSSRGPNT+  DILKPD+S PGVEILAA+
Sbjct: 414 LVSYINSTRSPQGSVLKTEAIFNQLSPKVASFSSRGPNTIAVDILKPDISAPGVEILAAY 473

Query: 65  PPVA-PVAKVQDSRSTLCNIISRMSMSSPHITGIAVYVKTFNPTWSTAAIKSALMTTA-- 124
            P++ P    +D R    +++S  SM+ PH+TG+A Y+KTF+P WS + I+SA+MTTA  
Sbjct: 474 SPLSLPSEDRRDKRRVKYSVLSGTSMACPHVTGVAAYIKTFHPDWSPSVIQSAIMTTAWQ 533

Query: 125 ------------------------------------------------------------ 184
                                                                       
Sbjct: 534 MNATGTGAESTEFAYGAGHVDPIAAINPGLVYELNKTDHISFLCGMNYTSKTLKLISGDA 593

Query: 185 -------------------------------------------STYTAIISAPQG--FEI 187
                                                      STY + I    G    +
Sbjct: 594 VICSGKTLQRNLNYPSMSAKLSESNSSFTVTFKRTVTNLGTANSTYKSKIVLNHGSKLNV 653

[Close]

BLAST of Cla97C07G132015 vs. ExPASy TrEMBL
Match: A0A5A7UDR3 (Cucumisin OS=Cucumis melo var. makuwa OX=1194695 GN=E6C27_scaffold174G001700 PE=3 SV=1)

HSP 1 Score: 227.3 bits (578), Expect = 5.5e-56
Identity = 140/286 (48.95%), Postives = 158/286 (55.24%), Query Frame = 0


Query: 8   YIDSARSPTATIFKSTTLHNVSAPVVISFSSRGPNTLTNDILKPDLSGPGVEILAAWPPV 67
           YI S RSP ATIFKSTT+ N SAPVV+SFSSRGPN  T D++KPD+SGPGVEILAAWP V
Sbjct: 398 YIYSIRSPGATIFKSTTILNASAPVVVSFSSRGPNRATKDVIKPDISGPGVEILAAWPSV 457

Query: 68  APVAKVQDSRSTLCNIISRMSMSSPHITGIAVYVKTFNPTWSTAAIKSALMTT------- 127
           APV  ++  R+TL NIIS  SMS PHITGIA YVKT+NPTWS AAIKSALMTT       
Sbjct: 458 APVGGIR--RNTLFNIISGTSMSCPHITGIATYVKTYNPTWSPAAIKSALMTTASPMNAR 517

Query: 128 ------------------------------------------------------------ 187
                                                                       
Sbjct: 518 FNPQAEFAYGSGHVNPLKAVRPGLVYDANESDYVKFLCGQGYNTEAVRRITGDYSACTPG 577

Query: 188 -------------------------------------ASTYTAIISAPQGFEITVDPNIL 190
                                                ASTY A+ISAPQG  I+V+PN+L
Sbjct: 578 NTGRVWDLNYPSFGLSVSPSKTFNQYFNRTLTSVAPQASTYRAMISAPQGLTISVNPNVL 637

[Close]

BLAST of Cla97C07G132015 vs. ExPASy TrEMBL
Match: A0A1S3CF86 (LOW QUALITY PROTEIN: cucumisin OS=Cucumis melo OX=3656 GN=LOC103500253 PE=3 SV=1)

HSP 1 Score: 226.1 bits (575), Expect = 1.2e-55
Identity = 140/285 (49.12%), Postives = 157/285 (55.09%), Query Frame = 0


Query: 8   YIDSARSPTATIFKSTTLHNVSAPVVISFSSRGPNTLTNDILKPDLSGPGVEILAAWPPV 67
           YI S RSP ATIFKSTT+ N SAPVV+SFSSRGPN  T D++KPD+SGPGVEILAAWP V
Sbjct: 447 YIYSIRSPGATIFKSTTILNASAPVVVSFSSRGPNRATKDVIKPDISGPGVEILAAWPSV 506

Query: 68  APVAKVQDSRSTLCNIISRMSMSSPHITGIAVYVKTFNPTWSTAAIKSALMTT------- 127
           APV  ++  R+TL NIIS  SMS PHITGIA YVKT+NPTWS AAIKSALMTT       
Sbjct: 507 APVGGIR--RNTLFNIISGTSMSCPHITGIATYVKTYNPTWSPAAIKSALMTTASPMNAR 566

Query: 128 ------------------------------------------------------------ 187
                                                                       
Sbjct: 567 FNPQAEFAYGSGHVNPLKAVRPGLVYDANESDYVKFLCGQGYNTEAVRRITGDYSACTPG 626

Query: 188 -------------------------------------ASTYTAIISAPQGFEITVDPNIL 189
                                                ASTY A+ISAPQG  I+V+PN+L
Sbjct: 627 NTGRVWDLNYPSFGLSVSPSKTFNQYFNRTLTSVAPQASTYRAMISAPQGLTISVNPNVL 686

[Close]

BLAST of Cla97C07G132015 vs. ExPASy TrEMBL
Match: A0A5D3E3U6 (Cucumisin OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold267G00160 PE=3 SV=1)

HSP 1 Score: 224.9 bits (572), Expect = 2.7e-55
Identity = 140/292 (47.95%), Postives = 158/292 (54.11%), Query Frame = 0


Query: 8   YIDSARSPTATIFKSTTLHNVSAPVVISFSSRGPNTLTNDILKPDLSGPGVEILAAWPPV 67
           YI S RSP ATIFKSTT+ N SAPVV+SFSSRGPN  T D++KPD+SGPGVEILAAWP V
Sbjct: 394 YIYSIRSPGATIFKSTTILNASAPVVVSFSSRGPNRATKDVIKPDISGPGVEILAAWPSV 453

Query: 68  APVAKVQDSRSTLCNIISRMSMSSPHITGIAVYVKTFNPTWSTAAIKSALMTT------- 127
           APV  ++  R+TL NIIS  SMS PHITGIA YVKT+NPTWS AAIKSALMTT       
Sbjct: 454 APVGGIR--RNTLFNIISGTSMSCPHITGIATYVKTYNPTWSPAAIKSALMTTANYFTLT 513

Query: 128 ------------------------------------------------------------ 187
                                                                       
Sbjct: 514 SPMNARFNPQAEFAYGSGHVNPLKAVRPGLVYDANESDYVKFLCGQGYNTEAVRRITGDY 573

Query: 188 -------------------------------------------ASTYTAIISAPQGFEIT 190
                                                      ASTY A+ISAPQG  I+
Sbjct: 574 SACTPGNTGRVWDLNYPSFGLSVSPSKTFNQYFNRTLTSVAPQASTYRAMISAPQGLTIS 633

[Close]

BLAST of Cla97C07G132015 vs. ExPASy TrEMBL
Match: A0A6J1GZY5 (cucumisin-like OS=Cucurbita moschata OX=3662 GN=LOC111458774 PE=3 SV=1)

HSP 1 Score: 224.6 bits (571), Expect = 3.6e-55
Identity = 143/290 (49.31%), Postives = 156/290 (53.79%), Query Frame = 0


Query: 3   IPIYDYIDSARSPTATIFKSTTLHNVSAPVVISFSSRGPNTLTNDILKPDLSGPGVEILA 62
           I +Y YI S   PTATIFKS    + SAPVV+SFSSRGPN LTNDILKPDLSGPGVEILA
Sbjct: 447 IAVYRYIRSTIIPTATIFKSVAGQDSSAPVVVSFSSRGPNALTNDILKPDLSGPGVEILA 506

Query: 63  AWPPVAPVAKVQDSRSTLCNIISRMSMSSPHITGIAVYVKTFNPTWSTAAIKSALMTTA- 122
           AWPPVAP+A V+DSRS+L NIIS  SMS PH+TG+AVYVKTFNPT S AAIKSALMTTA 
Sbjct: 507 AWPPVAPIAGVKDSRSSLYNIISGTSMSCPHLTGVAVYVKTFNPTLSPAAIKSALMTTAS 566

Query: 123 ------------------------------------------------------------ 182
                                                                       
Sbjct: 567 PMSARQHSQAEFAYGAGHVNPLKAINPGLVYDANESDYVSFLCGQGYDTAMVRRITGDNS 626

Query: 183 -------------------------------------------STYTAIISAPQGFEITV 189
                                                      STY A ISAPQG  ITV
Sbjct: 627 ACTSGNIGRVWDLNYPSFAVSISGTRTVNQYFTRTLTNVARGGSTYRAKISAPQGLSITV 686

[Close]

BLAST of Cla97C07G132015 vs. ExPASy TrEMBL
Match: K7NBW1 (Cucumisin OS=Siraitia grosvenorii OX=190515 PE=2 SV=1)

HSP 1 Score: 217.6 bits (553), Expect = 4.4e-53
Identity = 133/283 (47.00%), Postives = 155/283 (54.77%), Query Frame = 0


Query: 8   YIDSARSPTATIFKSTTLHNVSAPVVISFSSRGPNTLTNDILKPDLSGPGVEILAAWPPV 67
           Y+ S R+PTATIFKST + + SAPVV+SFSSRGPN +T+DILKPD + PGVEILAAWPPV
Sbjct: 449 YMSSTRAPTATIFKSTVVRDTSAPVVVSFSSRGPNYVTHDILKPDSTAPGVEILAAWPPV 508

Query: 68  APVAKVQDSRSTLCNIISRMSMSSPHITGIAVYVKTFNPTWSTAAIKSALMTT------- 127
           AP++ V+DSRS L NIIS  SMS PH+T IAV++KTF P+WS AAIKSALMTT       
Sbjct: 509 APISGVRDSRSALYNIISGTSMSCPHVTAIAVHIKTFYPSWSPAAIKSALMTTASPMNAR 568

Query: 128 ------------------------------------------------------------ 187
                                                                       
Sbjct: 569 FNSDAEFAYGSGHVNPLKAVDPGLVYDASESDYVKFLCGEGYTTAMVRSTTGDNSACTSG 628

[Close]

BLAST of Cla97C07G132015 vs. TAIR 10
Match: AT5G59120.1 (subtilase 4.13 )

HSP 1 Score: 129.4 bits (324), Expect = 3.0e-30
Identity = 90/293 (30.72%), Postives = 130/293 (44.37%), Query Frame = 0


Query: 5   IYDYIDSARSPTATIFKSTTLHNVSAPVVISFSSRGPNTLTNDILKPDLSGPGVEILAAW 64
           +  Y++S  SP A + K+  + N ++PV+ SFSSRGPNT+  DILKPD++ PGVEILAA+
Sbjct: 436 LVSYLESTDSPQAIVLKTEAIFNRTSPVIASFSSRGPNTIAVDILKPDITAPGVEILAAY 495

Query: 65  PPVAPVAKVQDSRSTLCNIISRMSMSSPHITGIAVYVKTFNPTWSTAAIKSALMTTA--- 124
            P    ++  D+R    +++S  SMS PH+ G+A YVKTFNP WS + I+SA+MTTA   
Sbjct: 496 SPAGEPSQ-DDTRHVKYSVLSGTSMSCPHVAGVAAYVKTFNPKWSPSMIQSAIMTTAWPV 555

Query: 125 ------------------------------------------------------------ 184
                                                                       
Sbjct: 556 NATGTGIASTEFAYGSGHVDPIAASNPGLVYELDKSDHIAFLCGMNYTSQVLKVISGETV 615

Query: 185 --------------------------------------------STYTAIISAPQG--FE 187
                                                       STYT+ + A  G   +
Sbjct: 616 TCSEAKKILPRNLNYPSMSAKLSGSGTTFTVTFNRTLTNVGTPNSTYTSKVVAGHGSKLD 675

[Close]

BLAST of Cla97C07G132015 vs. TAIR 10
Match: AT3G46850.1 (Subtilase family protein )

HSP 1 Score: 127.5 bits (319), Expect = 1.1e-29
Identity = 90/291 (30.93%), Postives = 130/291 (44.67%), Query Frame = 0


Query: 5   IYDYIDSARSPTATIFKSTTLHNVSAPVVISFSSRGPNTLTNDILKPDLSGPGVEILAAW 64
           +  Y++S ++P A + KS T+ N  APVV S+SSRGPN L +DILKPD++ PG EILAA+
Sbjct: 445 VLSYVNSTKNPKAAVLKSETIFNQKAPVVASYSSRGPNPLIHDILKPDITAPGSEILAAY 504

Query: 65  PPVAPVAKVQDSRSTLCNIISRMSMSSPHITGIAVYVKTFNPTWSTAAIKSALMTTA--- 124
            P  P ++  D+R     +IS  SMS PH+ G+A Y+KTF+P WS + I+SA+MTTA   
Sbjct: 505 SPYVPPSE-SDTRHVKYTVISGTSMSCPHVAGVAAYIKTFHPLWSPSMIQSAIMTTAWPM 564

Query: 125 ------------------------------------------------------------ 184
                                                                       
Sbjct: 565 NASTSPSNELAEFAYGAGHVDPIAAIHPGLVYEANKSDHITFLCGFNYTGKKLRLISGDS 624

Query: 185 -----------------STYTAIISAPQGFEIT--------------------------- 187
                             + +A +S  + F++T                           
Sbjct: 625 SSCTKEQTKSLTRNLNYPSMSAQVSGTKPFKVTFRRTVTNVGRPNATYKAKVVGSKLKVK 684

[Close]

BLAST of Cla97C07G132015 vs. TAIR 10
Match: AT5G58840.1 (Subtilase family protein )

HSP 1 Score: 123.6 bits (309), Expect = 1.7e-28
Identity = 86/292 (29.45%), Postives = 128/292 (43.84%), Query Frame = 0


Query: 5   IYDYIDSARSPTATIFKSTTLHNVSAPVVISFSSRGPNTLTNDILKPDLSGPGVEILAAW 64
           +  Y++S +SP  T+ KS  + N +AP V  FSSRGPNT+  DILKPD++ PGVEILAA+
Sbjct: 415 VISYVNSTKSPHGTVLKSEAIFNQAAPKVAGFSSRGPNTIAVDILKPDVTAPGVEILAAF 474

Query: 65  PPV-APVAKVQDSRSTLCNIISRMSMSSPHITGIAVYVKTFNPTWSTAAIKSALMTTA-- 124
            P+ +P    +D+R    +++S  SMS PH+ G+A Y+KTF+P WS + I+SA+MTTA  
Sbjct: 475 SPLNSPAQDKRDNRHVKYSVLSGTSMSCPHVAGVAAYIKTFHPEWSPSMIQSAIMTTAWP 534

Query: 125 ------------------------------------------------------------ 184
                                                                       
Sbjct: 535 MNATGTAVASTEFAYGAGHVDPIAAINPGLVYEIGKSDHIAFLCGLNYNATSLKLIAGEA 594

Query: 185 -------------------------------------------STYTA--IISAPQGFEI 187
                                                      STY +  +++     ++
Sbjct: 595 VTCTGKTLPRNLNYPSMSAKLPKSESSFIVTFNRTVTNVGTPNSTYKSKIVLNHGSNLKV 654

[Close]

BLAST of Cla97C07G132015 vs. TAIR 10
Match: AT5G58830.1 (Subtilisin-like serine endopeptidase family protein )

HSP 1 Score: 122.5 bits (306), Expect = 3.7e-28
Identity = 89/292 (30.48%), Postives = 126/292 (43.15%), Query Frame = 0


Query: 5   IYDYIDSARSPTATIFKSTTLHNVSAPVVISFSSRGPNTLTNDILKPDLSGPGVEILAAW 64
           +  YI+S RSP  ++ K+  + N  +P V SFSSRGPNT+  DILKPD+S PGVEILAA+
Sbjct: 405 LVSYINSTRSPQGSVLKTEAIFNQLSPKVASFSSRGPNTIAVDILKPDISAPGVEILAAY 464

Query: 65  PPVA-PVAKVQDSRSTLCNIISRMSMSSPHITGIAVYVKTFNPTWSTAAIKSALMTTA-- 124
            P++ P    +D R    +++S  SM+ PH+TG+A Y+KTF+P WS + I+SA+MTTA  
Sbjct: 465 SPLSLPSEDRRDKRRVKYSVLSGTSMACPHVTGVAAYIKTFHPDWSPSVIQSAIMTTAWQ 524

Query: 125 ------------------------------------------------------------ 184
                                                                       
Sbjct: 525 MNATGTGAESTEFAYGAGHVDPIAAINPGLVYELNKTDHISFLCGMNYTSKTLKLISGDA 584

Query: 185 -------------------------------------------STYTAIISAPQG--FEI 187
                                                      STY + I    G    +
Sbjct: 585 VICSGKTLQRNLNYPSMSAKLSESNSSFTVTFKRTVTNLGTANSTYKSKIVLNHGSKLNV 644

[Close]

BLAST of Cla97C07G132015 vs. TAIR 10
Match: AT5G59090.2 (subtilase 4.12 )

HSP 1 Score: 121.3 bits (303), Expect = 8.2e-28
Identity = 89/288 (30.90%), Postives = 126/288 (43.75%), Query Frame = 0


Query: 5   IYDYIDSARSPTATIFKSTTLHNVSAPVVISFSSRGPNTLTNDILKPDLSGPGVEILAAW 64
           +  YI+S  SP A + K+ T+ N ++PV+ SFSSRGPNT+  DILKPD++ PGVEILAA+
Sbjct: 437 LVSYIESQDSPQAAVLKTETIFNRTSPVIASFSSRGPNTIAVDILKPDITAPGVEILAAF 496

Query: 65  PPVAPVAKVQDSRSTLCNIISRMSMSSPHITGIAVYVKTFNPTWSTAAIKSALMTTA--- 124
            P    ++  D+R    ++ S  SM+ PH+ G+A YVKTF P WS + I+SA+MTTA   
Sbjct: 497 SPNGEPSE-DDTRRVKYSVFSGTSMACPHVAGVAAYVKTFYPRWSPSMIQSAIMTTAKGR 556

Query: 125 ------------------------------------------------------------ 184
                                                                       
Sbjct: 557 GIASTEFAYGAGHVDPMAALNPGLVYELDKADHIAFLCGMNYTSKTLKIISGDTVKCSKK 616

Query: 185 ---------------------------------------STYTAIISAPQG--FEITVDP 187
                                                  STY + + A  G    I V P
Sbjct: 617 NKILPRNLNYPSMSAKLSGTDSTFSVTFNRTLTNVGTPNSTYKSKVVAGHGSKLSIKVTP 676

The following BLAST results are available for this feature:

BLAST of Cla97C07G132015 vs. NCBI nr


Match Name	E-value	Identity	Description
KAA0051619.1	1.1e-55	48.95	cucumisin [Cucumis melo var. makuwa]	[more]
XP_038892506.1	1.5e-55	48.26	cucumisin-like [Benincasa hispida]	[more]
XP_008461721.1	2.5e-55	49.12	PREDICTED: LOW QUALITY PROTEIN: cucumisin [Cucumis melo]	[more]
TYK30793.1	5.7e-55	47.95	cucumisin [Cucumis melo var. makuwa]	[more]
3VTA_A	5.7e-55	49.12	Crystal Structure of cucumisin, a subtilisin-like endoprotease from Cucumis melo...	[more]

BLAST of Cla97C07G132015 vs. ExPASy Swiss-Prot


Match Name	E-value	Identity	Description
Q39547	7.4e-58	49.12	Cucumisin OS=Cucumis melo OX=3656 PE=1 SV=1	[more]
Q9FIG2	4.3e-29	30.72	Subtilisin-like protease SBT4.13 OS=Arabidopsis thaliana OX=3702 GN=SBT4.13 PE=2...	[more]
Q9STF7	1.6e-28	30.93	Subtilisin-like protease SBT4.6 OS=Arabidopsis thaliana OX=3702 GN=SBT4.6 PE=3 S...	[more]
Q9FIM5	2.3e-27	29.45	Subtilisin-like protease SBT4.9 OS=Arabidopsis thaliana OX=3702 GN=SBT4.9 PE=2 S...	[more]
Q9FIM6	5.2e-27	30.48	Subtilisin-like protease SBT4.8 OS=Arabidopsis thaliana OX=3702 GN=SBT4.8 PE=3 S...	[more]

BLAST of Cla97C07G132015 vs. ExPASy TrEMBL


Match Name	E-value	Identity	Description
A0A5A7UDR3	5.5e-56	48.95	Cucumisin OS=Cucumis melo var. makuwa OX=1194695 GN=E6C27_scaffold174G001700 PE=...	[more]
A0A1S3CF86	1.2e-55	49.12	LOW QUALITY PROTEIN: cucumisin OS=Cucumis melo OX=3656 GN=LOC103500253 PE=3 SV=1	[more]
A0A5D3E3U6	2.7e-55	47.95	Cucumisin OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold267G00160 PE=3...	[more]
A0A6J1GZY5	3.6e-55	49.31	cucumisin-like OS=Cucurbita moschata OX=3662 GN=LOC111458774 PE=3 SV=1	[more]
K7NBW1	4.4e-53	47.00	Cucumisin OS=Siraitia grosvenorii OX=190515 PE=2 SV=1	[more]

BLAST of Cla97C07G132015 vs. TAIR 10


Match Name	E-value	Identity	Description
AT5G59120.1	3.0e-30	30.72	subtilase 4.13	[more]
AT3G46850.1	1.1e-29	30.93	Subtilase family protein	[more]
AT5G58840.1	1.7e-28	29.45	Subtilase family protein	[more]
AT5G58830.1	3.7e-28	30.48	Subtilisin-like serine endopeptidase family protein	[more]
AT5G59090.2	8.2e-28	30.90	subtilase 4.12	[more]

InterPro

Analysis Name: InterPro Annotations of Watermelon (97103) v2.5
Date Performed: 2022-01-31


IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
IPR041469	Subtilisin-like protease, fibronectin type-III domain	PFAM	PF17766	fn3_6	coord: 119..186 e-value: 1.5E-16 score: 60.4
IPR000209	Peptidase S8/S53 domain	PFAM	PF00082	Peptidase_S8	coord: 31..125 e-value: 1.7E-12 score: 47.3
None	No IPR available	GENE3D	3.50.30.30		coord: 5..26 e-value: 1.5E-42 score: 148.0
None	No IPR available	PANTHER	PTHR10795:SF685	SUBTILISIN-LIKE PROTEASE SBT4.3	coord: 123..187
None	No IPR available	PANTHER	PTHR10795:SF685	SUBTILISIN-LIKE PROTEASE SBT4.3	coord: 7..126
None	No IPR available	PROSITE	PS51892	SUBTILASE	coord: 1..144 score: 9.069401
IPR036852	Peptidase S8/S53 domain superfamily	GENE3D	3.40.50.200	Peptidase S8/S53 domain	coord: 27..126 e-value: 1.5E-42 score: 148.0
IPR036852	Peptidase S8/S53 domain superfamily	SUPERFAMILY	52743	Subtilisin-like	coord: 25..126
IPR045051	Subtilisin-like protease	PANTHER	PTHR10795	PROPROTEIN CONVERTASE SUBTILISIN/KEXIN	coord: 123..187
IPR045051	Subtilisin-like protease	PANTHER	PTHR10795	PROPROTEIN CONVERTASE SUBTILISIN/KEXIN	coord: 7..126

Relationships

The following mRNA feature(s) are a part of this gene:


Feature Name	Unique Name	Type
Cla97C07G132015.1	Cla97C07G132015.1	mRNA

GO Annotation

GO Assignments

This gene is annotated with the following GO terms.

Category	Term Accession	Term Name
biological_process	GO:0006508	proteolysis
molecular_function	GO:0004252	serine-type endopeptidase activity
molecular_function	GO:0008236	serine-type peptidase activity