Cla97C06G118420 (gene) Watermelon (97103) v2.5

Overview
NameCla97C06G118420
Typegene
OrganismCitrullus lanatus subsp. vulgaris cv. 97103 (Watermelon (97103) v2.5)
DescriptionVacuolar-processing enzyme
LocationCla97Chr06: 12236341 .. 12239023 (+)
RNA-Seq ExpressionCla97C06G118420
SyntenyCla97C06G118420
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: exonCDSpolypeptide
Hold the cursor over a type above to highlight its positions in the sequence below.
ATGACAACTTGTGATACAAATCCAACAAAACTACTCATCATTCTCATAACTTTTCTGTTTTTCAACAACATTATTGCTAAGAAAGAGTGGATTAGTGATGCCACTACTAACCAAACTGGCAAGAGATGGGCACTTCTGATTGCGGGCTCCTATGGTTATGATAATTACAGGCATCAGGTACCCTTCTTATGCCCCTCCATTACACAACTGCACAACTTTGACGAGGCTGCTACAATGTGGTTGGCAATCCAGAAGCTTAAACAATTTAGTTTGGCTAGTGTATCCGTATCAGACACTTTGCCACATATCAAACGACATTTGTTAGAATAGTAGACATGTTAGATACTAGTTGTACAAAGACACATTAGGTTCAACATTTGTTAAACATGCATCAAACATGACATGTCTCTATATCTTGTATCTATGTCCCATCAAACTAATCTTTAACATGCATCAAACATGACGTGTCTATATGTCTTGTATCTAGTATCCAGCAAACTTTGAACTTCCTCCTTTTATAAAAATGATAAACTTTAGTAAACTTGTCTTTCTCACGTTCATGTCTTAGATTTAAAGAAAATGACATGTCATAGTGTCTCATATTTGTATCTATGCTTCCTAAGTTGACAATATATTTGTCCAACAATTATAGTATGGCAGCTTCGTTGAATGATTTTTCCAACTAGACACCCTCCTACTAATATATATATATATATATATTTTTTTTTTGTGATATAAAATCCAAAAACATAGGCGGATGTATGCCATGCATATCAAATACTGAAAAAGGGTGGAATCCCTGATGAAAACATAATAGTTTTCATGTATGACGATATTGCACTTGATCCAAACAATCCAAGGCAAGGATTCATTATCAACAAACCAGGTGGGCCTGATGTTTATCAAGGAGTTCCCAAGGACTATACAGGAAAGCATGTAAATGCAGTCAACTTTTATGCTGCCATTTTGGGAAACAAATCTGCTCTTACTGGAGGAAGTGGTAAGGTTATTCACAGTGGCCCTCATGATCATATTTTCATCTACTACACCGACCACGGTGCTGCTGGGATGCTTGGTAATGAATTACGTACATTTGCAACTCTTTTTCGTTTCAATAAATTAAGGGTATGTTTGAATTAACTTGTGCTAAGAGTAGGCAATTCAAACAGGTCGGCCTTAAACTCCCTTAGTATATTTGACTATATTTTGTTTCACTTGATCATAGGAATGCCTGAAGGTGATTATGTTTTCGTCAAGGATCTAATAGATGTGCTGAAAAGGAAACATGAAGCCAATAGTTACAAAAGCATGGTACTAAAATATATTCCATATATCATACAACAATAATTGCAAAGTTCAGATTATATATCAAAGTGAAGTAACAATTAGTTAACATGAAAATTTCAGGTAATATATGTGGAAGCATGTGAGTCTGGTAGTATGTTTGAAGGACAACTTCCAGAAAACATAAAGATATATGCAACAACGGCATCAAATGCAACAGAGGATAGTTGGGCAACATATTGTCCTAAAGAGTCTCGAAGTCCTCCTCCCAAAGGTTATAACACTTGTCTGGGAGATTTGTACAGCATCGCTTGGATGGAAGACAGGCAATATTTTCTTTACACTTCTATCTGTGTTTAAAGTACTGATATAATTAAATTTTACAGTAACTTTTCAGCTTAAACTTTTGAGTTCAGTTGTATTTTAACATGATATCGGAGTAAAAGTGTTGAACGTCATTTTCAAGCTACACAGTCATTCTCCAATCATCTTAAATGAATTTGTGTTATGTCATGATGTGCAGTGACAAGCATGATTCGAGTAAGGAAACTTTGATCCAACAATATCATGCAGTAAGCTTCCTAATTCTTAAATGAGCTCAATTTTCTTAAATCACTCTACTGATAAACATTTCCTTGTACATTCCGAAAACTCCAGGTTCGAAGAAGAACTATGGTTGATAAAAGTGGCTACGGTTCTCATGTAATGTTGTATGGAAACAAAAGGATCGGCAACAATTCCCTTAACACCTATATTGGTAATAGCCCTGATTATAACAACTATAAATCCTCTGTGAAGAGTACTCAAACCATTTCTTCACCATCAAAATTGTCATTTAATGTGGTAAGCCAGCGTGACGCTAGTCTCATTTATTATCAGTACAAGGTATGATTTGTTAAACAATGTATATATTCTAACTATGATTCCCATCTACTACATGGTCTAAGTGTGTAGACTTTTGCTTTGATCAGTTTCAGCAAGCTCCATTTGGATCTAGAGAAAAGATGGAGGCTAGAAAGCAGCTAGAGGATGAAATTATGAAAAGAAGGCATGCTGACAACAGCATAAATAACATTGGTAGAATTTTGTTTGGACAAGCAAGGAGCTCAAAAGTGCTTAACAAAGTTAGGGAGCAAGGGCAATCCCTGGTTGATGATTGGGACTGCTTTAAAACATTTGTAAGTCCTATTTTTCAAGTCCCTACTTATTTTATACCATCAGAAACTTTGTTGATGCAATAATCTCCATAACACAGGTGAAAATCTATGAGAAACATTGCGAAAGAATGTCAAGCTATGGGATGAAATACGCACGAGCTATAGCCAATATATGCAATGCTGGGATTACAATGCACCAAATGGATCAGGCTTGTCTCCAAACTTGCCTTGGAAAGACCTGA

mRNA sequence

ATGACAACTTGTGATACAAATCCAACAAAACTACTCATCATTCTCATAACTTTTCTGTTTTTCAACAACATTATTGCTAAGAAAGAGTGGATTAGTGATGCCACTACTAACCAAACTGGCAAGAGATGGGCACTTCTGATTGCGGGCTCCTATGGTTATGATAATTACAGGCATCAGGCGGATGTATGCCATGCATATCAAATACTGAAAAAGGGTGGAATCCCTGATGAAAACATAATAGTTTTCATGTATGACGATATTGCACTTGATCCAAACAATCCAAGGCAAGGATTCATTATCAACAAACCAGGTGGGCCTGATGTTTATCAAGGAGTTCCCAAGGACTATACAGGAAAGCATGTAAATGCAGTCAACTTTTATGCTGCCATTTTGGGAAACAAATCTGCTCTTACTGGAGGAAGTGGTAAGGTTATTCACAGTGGCCCTCATGATCATATTTTCATCTACTACACCGACCACGGTGCTGCTGGGATGCTTGGAATGCCTGAAGGTGATTATGTTTTCGTCAAGGATCTAATAGATGTGCTGAAAAGGAAACATGAAGCCAATAGTTACAAAAGCATGGTAATATATGTGGAAGCATGTGAGTCTGGTAGTATGTTTGAAGGACAACTTCCAGAAAACATAAAGATATATGCAACAACGGCATCAAATGCAACAGAGGATAGTTGGGCAACATATTGTCCTAAAGAGTCTCGAAGTCCTCCTCCCAAAGGTTATAACACTTGTCTGGGAGATTTGTACAGCATCGCTTGGATGGAAGACAGTGACAAGCATGATTCGAGTAAGGAAACTTTGATCCAACAATATCATGCAGTTCGAAGAAGAACTATGGTTGATAAAAGTGGCTACGGTTCTCATGTAATGTTGTATGGAAACAAAAGGATCGGCAACAATTCCCTTAACACCTATATTGGTAATAGCCCTGATTATAACAACTATAAATCCTCTGTGAAGAGTACTCAAACCATTTCTTCACCATCAAAATTGTCATTTAATGTGGTAAGCCAGCGTGACGCTAGTCTCATTTATTATCAGTACAAGTTTCAGCAAGCTCCATTTGGATCTAGAGAAAAGATGGAGGCTAGAAAGCAGCTAGAGGATGAAATTATGAAAAGAAGGCATGCTGACAACAGCATAAATAACATTGGTAGAATTTTGTTTGGACAAGCAAGGAGCTCAAAAGTGCTTAACAAAGTTAGGGAGCAAGGGCAATCCCTGGTTGATGATTGGGACTGCTTTAAAACATTTGTGAAAATCTATGAGAAACATTGCGAAAGAATGTCAAGCTATGGGATGAAATACGCACGAGCTATAGCCAATATATGCAATGCTGGGATTACAATGCACCAAATGGATCAGGCTTGTCTCCAAACTTGCCTTGGAAAGACCTGA

Coding sequence (CDS)

ATGACAACTTGTGATACAAATCCAACAAAACTACTCATCATTCTCATAACTTTTCTGTTTTTCAACAACATTATTGCTAAGAAAGAGTGGATTAGTGATGCCACTACTAACCAAACTGGCAAGAGATGGGCACTTCTGATTGCGGGCTCCTATGGTTATGATAATTACAGGCATCAGGCGGATGTATGCCATGCATATCAAATACTGAAAAAGGGTGGAATCCCTGATGAAAACATAATAGTTTTCATGTATGACGATATTGCACTTGATCCAAACAATCCAAGGCAAGGATTCATTATCAACAAACCAGGTGGGCCTGATGTTTATCAAGGAGTTCCCAAGGACTATACAGGAAAGCATGTAAATGCAGTCAACTTTTATGCTGCCATTTTGGGAAACAAATCTGCTCTTACTGGAGGAAGTGGTAAGGTTATTCACAGTGGCCCTCATGATCATATTTTCATCTACTACACCGACCACGGTGCTGCTGGGATGCTTGGAATGCCTGAAGGTGATTATGTTTTCGTCAAGGATCTAATAGATGTGCTGAAAAGGAAACATGAAGCCAATAGTTACAAAAGCATGGTAATATATGTGGAAGCATGTGAGTCTGGTAGTATGTTTGAAGGACAACTTCCAGAAAACATAAAGATATATGCAACAACGGCATCAAATGCAACAGAGGATAGTTGGGCAACATATTGTCCTAAAGAGTCTCGAAGTCCTCCTCCCAAAGGTTATAACACTTGTCTGGGAGATTTGTACAGCATCGCTTGGATGGAAGACAGTGACAAGCATGATTCGAGTAAGGAAACTTTGATCCAACAATATCATGCAGTTCGAAGAAGAACTATGGTTGATAAAAGTGGCTACGGTTCTCATGTAATGTTGTATGGAAACAAAAGGATCGGCAACAATTCCCTTAACACCTATATTGGTAATAGCCCTGATTATAACAACTATAAATCCTCTGTGAAGAGTACTCAAACCATTTCTTCACCATCAAAATTGTCATTTAATGTGGTAAGCCAGCGTGACGCTAGTCTCATTTATTATCAGTACAAGTTTCAGCAAGCTCCATTTGGATCTAGAGAAAAGATGGAGGCTAGAAAGCAGCTAGAGGATGAAATTATGAAAAGAAGGCATGCTGACAACAGCATAAATAACATTGGTAGAATTTTGTTTGGACAAGCAAGGAGCTCAAAAGTGCTTAACAAAGTTAGGGAGCAAGGGCAATCCCTGGTTGATGATTGGGACTGCTTTAAAACATTTGTGAAAATCTATGAGAAACATTGCGAAAGAATGTCAAGCTATGGGATGAAATACGCACGAGCTATAGCCAATATATGCAATGCTGGGATTACAATGCACCAAATGGATCAGGCTTGTCTCCAAACTTGCCTTGGAAAGACCTGA

Protein sequence

MTTCDTNPTKLLIILITFLFFNNIIAKKEWISDATTNQTGKRWALLIAGSYGYDNYRHQADVCHAYQILKKGGIPDENIIVFMYDDIALDPNNPRQGFIINKPGGPDVYQGVPKDYTGKHVNAVNFYAAILGNKSALTGGSGKVIHSGPHDHIFIYYTDHGAAGMLGMPEGDYVFVKDLIDVLKRKHEANSYKSMVIYVEACESGSMFEGQLPENIKIYATTASNATEDSWATYCPKESRSPPPKGYNTCLGDLYSIAWMEDSDKHDSSKETLIQQYHAVRRRTMVDKSGYGSHVMLYGNKRIGNNSLNTYIGNSPDYNNYKSSVKSTQTISSPSKLSFNVVSQRDASLIYYQYKFQQAPFGSREKMEARKQLEDEIMKRRHADNSINNIGRILFGQARSSKVLNKVREQGQSLVDDWDCFKTFVKIYEKHCERMSSYGMKYARAIANICNAGITMHQMDQACLQTCLGKT
Homology
BLAST of Cla97C06G118420 vs. NCBI nr
Match: XP_038880044.1 (vacuolar-processing enzyme-like [Benincasa hispida])

HSP 1 Score: 875.5 bits (2261), Expect = 2.0e-250
Identity = 422/471 (89.60%), Postives = 443/471 (94.06%), Query Frame = 0

Query: 1   MTTCDTNPTKLLIILITFLFFNNIIAKKEWISDATTNQTGKRWALLIAGSYGYDNYRHQA 60
           MTTC+ +PTKLL+ILI F+FFNNIIAKKEWISDAT NQTGKRWALL+AGS GY+NYRHQA
Sbjct: 1   MTTCNRSPTKLLVILIIFVFFNNIIAKKEWISDATANQTGKRWALLVAGSNGYENYRHQA 60

Query: 61  DVCHAYQILKKGGIPDENIIVFMYDDIALDPNNPRQGFIINKPGGPDVYQGVPKDYTGKH 120
           DVCHAYQILKKGGIPDENIIVFMYDDIA  PNNPRQG IINKPGGPDVYQGVPKDYTGK 
Sbjct: 61  DVCHAYQILKKGGIPDENIIVFMYDDIAFHPNNPRQGIIINKPGGPDVYQGVPKDYTGKQ 120

Query: 121 VNAVNFYAAILGNKSALTGGSGKVIHSGPHDHIFIYYTDHGAAGMLGMPEGDYVFVKDLI 180
           VNAVNFYAA+ GNKSALTGGSGKVIHSGPHDHIFIYY+DHGAAGMLGMPE DYVFV DLI
Sbjct: 121 VNAVNFYAALSGNKSALTGGSGKVIHSGPHDHIFIYYSDHGAAGMLGMPEDDYVFVNDLI 180

Query: 181 DVLKRKHEANSYKSMVIYVEACESGSMFEGQLPENIKIYATTASNATEDSWATYCPKESR 240
           D LKRKH+A SYKSMVIYVEACESGSMFEG LPENIKIYATTASNATEDSWATYCPKESR
Sbjct: 181 DALKRKHKAKSYKSMVIYVEACESGSMFEGLLPENIKIYATTASNATEDSWATYCPKESR 240

Query: 241 SPPPKGYNTCLGDLYSIAWMEDSDKHDSSKETLIQQYHAVRRRTMVDKSGYGSHVMLYGN 300
            PPPK YNTCLGDLYS+AWMEDSDKHD SKETL QQYHAVRRRT+VDKSG GSHVMLYGN
Sbjct: 241 GPPPKDYNTCLGDLYSVAWMEDSDKHDLSKETLNQQYHAVRRRTLVDKSGVGSHVMLYGN 300

Query: 301 KRIGNNSLNTYIGNSPDYNNYKSSVKSTQTISSPSKLSFNVVSQRDASLIYYQYKFQQAP 360
           +RIG + LNT+IGNSPDYNNYKSSV+S  TISSPSKLS N VSQRDASLIYYQYKFQ+AP
Sbjct: 301 RRIGTDFLNTFIGNSPDYNNYKSSVQSIHTISSPSKLSSNGVSQRDASLIYYQYKFQKAP 360

Query: 361 FGSREKMEARKQLEDEIMKRRHADNSINNIGRILFGQARSSKVLNKVREQGQSLVDDWDC 420
            GSREKMEAR+QLEDEI+KRRHADNSINNIG++LFGQARSSKVLNKVREQGQSLVDDW C
Sbjct: 361 IGSREKMEAREQLEDEILKRRHADNSINNIGKLLFGQARSSKVLNKVREQGQSLVDDWHC 420

Query: 421 FKTFVKIYEKHCERMSSYGMKYARAIANICNAGITMHQMDQACLQTCLGKT 472
           FKTFVKIYEKHC+RMSSYGMKY RAIANICNAGITMHQMDQACLQTCLGKT
Sbjct: 421 FKTFVKIYEKHCKRMSSYGMKYTRAIANICNAGITMHQMDQACLQTCLGKT 471

BLAST of Cla97C06G118420 vs. NCBI nr
Match: KAA0046547.1 (vacuolar-processing enzyme gamma-isozyme-like [Cucumis melo var. makuwa])

HSP 1 Score: 807.4 bits (2084), Expect = 6.7e-230
Identity = 399/471 (84.71%), Postives = 423/471 (89.81%), Query Frame = 0

Query: 1   MTTCDTNPTKLLIILITFLFFNNIIAKKEWISDATTNQTGKRWALLIAGSYGYDNYRHQA 60
           MTTCD NPTKLLIILITFL FNNIIAK EWISDATT+QTGK WALLIAGS GYDNYRHQ 
Sbjct: 1   MTTCDRNPTKLLIILITFLCFNNIIAKNEWISDATTDQTGKIWALLIAGSNGYDNYRHQ- 60

Query: 61  DVCHAYQILKKGGIPDENIIVFMYDDIALDPNNPRQGFIINKPGGPDVYQGVPKDYTGKH 120
                      GGIPDENIIVFMYDDIA  P+NPR G IINKP G DVY GVPKDYTGKH
Sbjct: 61  -----------GGIPDENIIVFMYDDIAFHPSNPRPGIIINKPDGNDVYHGVPKDYTGKH 120

Query: 121 VNAVNFYAAILGNKSALTGGSGKVIHSGPHDHIFIYYTDHGAAGMLGMPEGDYVFVKDLI 180
           VNAVNFYAAILGNKSALTGGSGKVIHSGP DHIFIYY+DHGAAGMLGMPEGDY+FV DLI
Sbjct: 121 VNAVNFYAAILGNKSALTGGSGKVIHSGPQDHIFIYYSDHGAAGMLGMPEGDYIFVNDLI 180

Query: 181 DVLKRKHEANSYKSMVIYVEACESGSMFEGQLPENIKIYATTASNATEDSWATYCPKESR 240
           DVLKRKH+A +YKSMVIYVEACESGSMFEG LPENIKIYATTASNATEDSWATYCPK+S 
Sbjct: 181 DVLKRKHKAKNYKSMVIYVEACESGSMFEGVLPENIKIYATTASNATEDSWATYCPKQSH 240

Query: 241 SPPPKGYNTCLGDLYSIAWMEDSDKHDSSKETLIQQYHAVRRRTMVDKSGYGSHVMLYGN 300
              PK YNTCLGDL+S+AWMEDSDKHD SKETL QQYHAVRRRT VDKSGYGSHVMLYGN
Sbjct: 241 R-SPKDYNTCLGDLFSVAWMEDSDKHDLSKETLNQQYHAVRRRTFVDKSGYGSHVMLYGN 300

Query: 301 KRIGNNSLNTYIGNSPDYNNYKSSVKSTQTISSPSKLSFNVVSQRDASLIYYQYKFQQAP 360
           KRIGN+ L+T++GNSPDY+NYK SV+S QTISS SKLS N VSQRDASLI+YQYKFQ+AP
Sbjct: 301 KRIGNDFLDTFLGNSPDYDNYKYSVQSPQTISSLSKLSSNSVSQRDASLIHYQYKFQEAP 360

Query: 361 FGSREKMEARKQLEDEIMKRRHADNSINNIGRILFGQARSSKVLNKVREQGQSLVDDWDC 420
           FGSREKMEAR+QLEDEI KRRHADNSIN+IG++LFGQARSS++L+ VR QGQSLVDDW C
Sbjct: 361 FGSREKMEAREQLEDEIFKRRHADNSINSIGKLLFGQARSSEILHNVRAQGQSLVDDWHC 420

Query: 421 FKTFVKIYEKHCERMSSYGMKYARAIANICNAGITMHQMDQACLQTCLGKT 472
           FKTFVKIYEKHC RMSSYGMKY RAIANICNAGITMHQMDQACLQTCLGKT
Sbjct: 421 FKTFVKIYEKHCNRMSSYGMKYTRAIANICNAGITMHQMDQACLQTCLGKT 458

BLAST of Cla97C06G118420 vs. NCBI nr
Match: TYK28710.1 (vacuolar-processing enzyme gamma-isozyme-like [Cucumis melo var. makuwa])

HSP 1 Score: 780.4 bits (2014), Expect = 8.7e-222
Identity = 391/471 (83.01%), Postives = 413/471 (87.69%), Query Frame = 0

Query: 1   MTTCDTNPTKLLIILITFLFFNNIIAKKEWISDATTNQTGKRWALLIAGSYGYDNYRHQA 60
           MTTCD NPTKLLIILITFL FNNIIAK EWISDATT+QTGK WALLIAGS GYDNYRHQ 
Sbjct: 1   MTTCDRNPTKLLIILITFLCFNNIIAKNEWISDATTDQTGKIWALLIAGSNGYDNYRHQ- 60

Query: 61  DVCHAYQILKKGGIPDENIIVFMYDDIALDPNNPRQGFIINKPGGPDVYQGVPKDYTGKH 120
                      GGIPDENIIVFMYDDIA  P+NPR G IINKP G DVY GVPKDYTGKH
Sbjct: 61  -----------GGIPDENIIVFMYDDIAFHPSNPRPGIIINKPDGNDVYHGVPKDYTGKH 120

Query: 121 VNAVNFYAAILGNKSALTGGSGKVIHSGPHDHIFIYYTDHGAAGMLGMPEGDYVFVKDLI 180
           VNAVNFYAAILGNKSALTGGSGKVIHSGP DHIFIYY+DHGAAGMLGMPEGDY+FV DLI
Sbjct: 121 VNAVNFYAAILGNKSALTGGSGKVIHSGPQDHIFIYYSDHGAAGMLGMPEGDYIFVNDLI 180

Query: 181 DVLKRKHEANSYKSMVIYVEACESGSMFEGQLPENIKIYATTASNATEDSWATYCPKESR 240
           DVLKRKH+A +YKSMVIYVEACESGSMFEG LPENIKIYATTASNATEDSWATYCPK+S 
Sbjct: 181 DVLKRKHKAKNYKSMVIYVEACESGSMFEGVLPENIKIYATTASNATEDSWATYCPKQSH 240

Query: 241 SPPPKGYNTCLGDLYSIAWMEDSDKHDSSKETLIQQYHAVRRRTMVDKSGYGSHVMLYGN 300
              PK YNTCLGD          DKHD SKETL QQYHAVRRRT VDKSGYGSHVMLYGN
Sbjct: 241 R-SPKDYNTCLGDF---------DKHDLSKETLNQQYHAVRRRTFVDKSGYGSHVMLYGN 300

Query: 301 KRIGNNSLNTYIGNSPDYNNYKSSVKSTQTISSPSKLSFNVVSQRDASLIYYQYKFQQAP 360
           KRIGN+ L+T++GNSPDY+NYK SV+S QTISS SKLS N VSQRDASLI+YQYKFQ+AP
Sbjct: 301 KRIGNDFLDTFLGNSPDYDNYKYSVQSPQTISSLSKLSSNSVSQRDASLIHYQYKFQEAP 360

Query: 361 FGSREKMEARKQLEDEIMKRRHADNSINNIGRILFGQARSSKVLNKVREQGQSLVDDWDC 420
           FGSREKMEAR+QLEDEI KRRHADNSIN+IG++LFGQARSS++L+ VR QGQSLVDDW C
Sbjct: 361 FGSREKMEAREQLEDEIFKRRHADNSINSIGKLLFGQARSSEILHNVRAQGQSLVDDWHC 420

Query: 421 FKTFVKIYEKHCERMSSYGMKYARAIANICNAGITMHQMDQACLQTCLGKT 472
           FKTFVKIYEKHC RMSSYGMKY RAIANICNAGITMHQMDQACLQTCLGKT
Sbjct: 421 FKTFVKIYEKHCNRMSSYGMKYTRAIANICNAGITMHQMDQACLQTCLGKT 449

BLAST of Cla97C06G118420 vs. NCBI nr
Match: XP_023516171.1 (vacuolar-processing enzyme gamma-isozyme-like [Cucurbita pepo subsp. pepo])

HSP 1 Score: 746.1 bits (1925), Expect = 1.8e-211
Identity = 367/471 (77.92%), Postives = 406/471 (86.20%), Query Frame = 0

Query: 1   MTTCDTNPTKLLIILITFLFFNNIIAKKEWISDATTNQTGKRWALLIAGSYGYDNYRHQA 60
           MTT  T PT LL  LI  LFFN+IIAKK+W+S A  +Q GKRWALL+AGS GYDNYRHQA
Sbjct: 1   MTTFATTPTTLL--LIALLFFNSIIAKKQWVSHANADQPGKRWALLVAGSNGYDNYRHQA 60

Query: 61  DVCHAYQILKKGGIPDENIIVFMYDDIALDPNNPRQGFIINKPGGPDVYQGVPKDYTGKH 120
           DVCHAYQIL+K GIPDENIIVFMYDDIA DP+NPR G IINKPGG DVYQGVPKDYTGK 
Sbjct: 61  DVCHAYQILRK-GIPDENIIVFMYDDIAFDPDNPRPGIIINKPGGADVYQGVPKDYTGKD 120

Query: 121 VNAVNFYAAILGNKSALTGGSGKVIHSGPHDHIFIYYTDHGAAGMLGMPEGDYVFVKDLI 180
           VN+VNFYAAILGNKS +TGGSGKVI SGP+DHIFIYYTDHGAAGMLGMP GDY++  DL+
Sbjct: 121 VNSVNFYAAILGNKSGVTGGSGKVIDSGPNDHIFIYYTDHGAAGMLGMPMGDYIYSNDLM 180

Query: 181 DVLKRKHEANSYKSMVIYVEACESGSMFEGQLPENIKIYATTASNATEDSWATYCPKESR 240
           DVLKRKHEA SYKSMVIYVEACESGSMFEG LPEN+ IYATTASNATEDSWA YCP +S 
Sbjct: 181 DVLKRKHEAKSYKSMVIYVEACESGSMFEGLLPENMNIYATTASNATEDSWAAYCPGQSP 240

Query: 241 SPPPKGYNTCLGDLYSIAWMEDSDKHDSSKETLIQQYHAVRRRTMVDKSGYGSHVMLYGN 300
           + P  GY+TCLGDLYS+AWMEDSDKHDSSKETL QQYH VRRRT VDK GYGSHVMLYGN
Sbjct: 241 T-PHTGYDTCLGDLYSVAWMEDSDKHDSSKETLNQQYHTVRRRTSVDKVGYGSHVMLYGN 300

Query: 301 KRIGNNSLNTYIGNSPDYNNYKSSVKSTQTISSPSKLSFNVVSQRDASLIYYQYKFQQAP 360
           + IGNN LNTY GNS D NN +SSVKST  ISS +    N VSQRDASL++Y YKFQ AP
Sbjct: 301 ESIGNNFLNTYFGNSSDDNNNESSVKSTHIISSLA----NGVSQRDASLLHYWYKFQNAP 360

Query: 361 FGSREKMEARKQLEDEIMKRRHADNSINNIGRILFGQARSSKVLNKVREQGQSLVDDWDC 420
           +GSREKMEA ++LEDEI+ RRHADNSIN IG +LFG+A+SS+VLN++R  GQS VDDW+C
Sbjct: 361 YGSREKMEASERLEDEILSRRHADNSINQIGELLFGEAKSSRVLNRIRGHGQSFVDDWNC 420

Query: 421 FKTFVKIYEKHCERMSSYGMKYARAIANICNAGITMHQMDQACLQTCLGKT 472
           FK +VKIYEKHC+RMS YGMKY +A+ANICNAGITM QMDQACLQTC+G T
Sbjct: 421 FKKYVKIYEKHCKRMSRYGMKYTQALANICNAGITMDQMDQACLQTCVGST 463

BLAST of Cla97C06G118420 vs. NCBI nr
Match: XP_022961313.1 (vacuolar-processing enzyme gamma-isozyme-like [Cucurbita moschata])

HSP 1 Score: 741.1 bits (1912), Expect = 5.9e-210
Identity = 363/471 (77.07%), Postives = 407/471 (86.41%), Query Frame = 0

Query: 1   MTTCDTNPTKLLIILITFLFFNNIIAKKEWISDATTNQTGKRWALLIAGSYGYDNYRHQA 60
           MTT  T PT LL  LI  LFFN+IIAKK+W+S A T+Q GKRWALL+AGS GYDNYRHQA
Sbjct: 19  MTTFATTPTTLL--LIALLFFNSIIAKKQWVSHANTDQPGKRWALLVAGSNGYDNYRHQA 78

Query: 61  DVCHAYQILKKGGIPDENIIVFMYDDIALDPNNPRQGFIINKPGGPDVYQGVPKDYTGKH 120
           DVCHAYQIL+K GIPDENIIVFMYDDIAL P+NPR G IINKPGG DVYQGVPKDYTGK 
Sbjct: 79  DVCHAYQILRK-GIPDENIIVFMYDDIALHPDNPRPGIIINKPGGADVYQGVPKDYTGKD 138

Query: 121 VNAVNFYAAILGNKSALTGGSGKVIHSGPHDHIFIYYTDHGAAGMLGMPEGDYVFVKDLI 180
           VN+VNFYAAILGNKS +TGGSGKVI SGP+DHIFIYYTDHGAAGMLGMP GDY++  DL+
Sbjct: 139 VNSVNFYAAILGNKSGVTGGSGKVIDSGPNDHIFIYYTDHGAAGMLGMPMGDYIYSNDLM 198

Query: 181 DVLKRKHEANSYKSMVIYVEACESGSMFEGQLPENIKIYATTASNATEDSWATYCPKESR 240
           DVLKRKHEA SYKSMVIYVEACE+GS+FEG LPEN+ IYATTASNATEDSWA YCP +S 
Sbjct: 199 DVLKRKHEAKSYKSMVIYVEACEAGSLFEGLLPENMNIYATTASNATEDSWAAYCPGQSP 258

Query: 241 SPPPKGYNTCLGDLYSIAWMEDSDKHDSSKETLIQQYHAVRRRTMVDKSGYGSHVMLYGN 300
           + P  GY+TCLGDLYS+AWMEDSDKHDSSKETL QQYH VRRRT VDK GYGSHVMLYGN
Sbjct: 259 T-PHTGYDTCLGDLYSVAWMEDSDKHDSSKETLNQQYHTVRRRTSVDKVGYGSHVMLYGN 318

Query: 301 KRIGNNSLNTYIGNSPDYNNYKSSVKSTQTISSPSKLSFNVVSQRDASLIYYQYKFQQAP 360
           + IGNN LNTY GN+ D NN +SSVKST  ISS S    N VSQRDASL++Y YKFQ AP
Sbjct: 319 ESIGNNFLNTYFGNTSDDNNNESSVKSTHIISSLS----NGVSQRDASLLHYWYKFQNAP 378

Query: 361 FGSREKMEARKQLEDEIMKRRHADNSINNIGRILFGQARSSKVLNKVREQGQSLVDDWDC 420
           +GSREKMEA ++LEDEI+ RRHADNS+N IG +LFG+A+SS+VLN++R  GQS VDDW+C
Sbjct: 379 YGSREKMEASERLEDEILSRRHADNSVNQIGELLFGEAKSSRVLNRIRGHGQSFVDDWNC 438

Query: 421 FKTFVKIYEKHCERMSSYGMKYARAIANICNAGITMHQMDQACLQTCLGKT 472
           FK +VKIYEKHC+RMS YGMKY +A+ANICNAGIT+ QMD+ACLQTC+G T
Sbjct: 439 FKKYVKIYEKHCKRMSRYGMKYTQALANICNAGITIDQMDKACLQTCVGNT 481

BLAST of Cla97C06G118420 vs. ExPASy Swiss-Prot
Match: Q39119 (Vacuolar-processing enzyme gamma-isozyme OS=Arabidopsis thaliana OX=3702 GN=At4g32940 PE=1 SV=2)

HSP 1 Score: 510.4 bits (1313), Expect = 2.2e-143
Identity = 245/431 (56.84%), Postives = 315/431 (73.09%), Query Frame = 0

Query: 37  NQTGKRWALLIAGSYGYDNYRHQADVCHAYQILKKGGIPDENIIVFMYDDIALDPNNPRQ 96
           + +G RWA+L+AGS GY NYRHQAD+CHAYQ+L+KGG+ +ENI+VFMYDDIA +  NPR 
Sbjct: 54  SNSGTRWAVLVAGSSGYWNYRHQADICHAYQLLRKGGLKEENIVVFMYDDIANNYENPRP 113

Query: 97  GFIINKPGGPDVYQGVPKDYTGKHVNAVNFYAAILGNKSALTGGSGKVIHSGPHDHIFIY 156
           G IIN P G DVYQGVPKDYTG  VN  N +A ILG+K+A+ GGSGKV+ SGP+DHIFI+
Sbjct: 114 GTIINSPHGKDVYQGVPKDYTGDDVNVDNLFAVILGDKTAVKGGSGKVVDSGPNDHIFIF 173

Query: 157 YTDHGAAGMLGMPEGDYVFVKDLIDVLKRKHEANSYKSMVIYVEACESGSMFEGQLPENI 216
           Y+DHG  G+LGMP   Y++  DL DVLK+KH   +YKS+V Y+EACESGS+FEG LPE +
Sbjct: 174 YSDHGGPGVLGMPTSPYLYANDLNDVLKKKHALGTYKSLVFYLEACESGSIFEGLLPEGL 233

Query: 217 KIYATTASNATEDSWATYCPKESRSPPPKGYNTCLGDLYSIAWMEDSDKHDSSKETLIQQ 276
            IYATTASNA E SW TYCP E  SPPP+ Y TCLGDLYS+AWMEDS  H+   ETL QQ
Sbjct: 234 NIYATTASNAEESSWGTYCPGEEPSPPPE-YETCLGDLYSVAWMEDSGMHNLQTETLHQQ 293

Query: 277 YHAVRRRTMVDKSGYGSHVMLYGNKRIGNNSLNTYIGNSPDYNNYKSSVKSTQTISSPSK 336
           Y  V+RRT      YGSHVM YG+  I  ++L+ Y+G +P  +N+  +     ++  PS+
Sbjct: 294 YELVKRRTAPVGYSYGSHVMQYGDVGISKDNLDLYMGTNPANDNF--TFADANSLKPPSR 353

Query: 337 LSFNVVSQRDASLIYYQYKFQQAPFGSREKMEARKQLEDEIMKRRHADNSINNIGRILFG 396
               V +QRDA L+++  K+++AP GS  K EA+KQ+ + +  R H DNS+  +G+ILFG
Sbjct: 354 ----VTNQRDADLVHFWEKYRKAPEGSARKTEAQKQVLEAMSHRLHIDNSVILVGKILFG 413

Query: 397 QARSSKVLNKVREQGQSLVDDWDCFKTFVKIYEKHCERMSSYGMKYARAIANICNAGITM 456
            +R  +VLNKVR  GQ LVDDW+C K  V+ +E+HC  +S YG+K+ R+ ANICNAGI M
Sbjct: 414 ISRGPEVLNKVRSAGQPLVDDWNCLKNQVRAFERHCGSLSQYGIKHMRSFANICNAGIQM 473

Query: 457 HQMDQACLQTC 468
            QM++A  Q C
Sbjct: 474 EQMEEAASQAC 477

BLAST of Cla97C06G118420 vs. ExPASy Swiss-Prot
Match: O24325 (Vacuolar-processing enzyme OS=Phaseolus vulgaris OX=3885 PE=1 SV=1)

HSP 1 Score: 507.3 bits (1305), Expect = 1.9e-142
Identity = 253/472 (53.60%), Postives = 331/472 (70.13%), Query Frame = 0

Query: 6   TNPTKLLIILITFLFFNNIIAKKEWISDAT---------TNQTGKRWALLIAGSYGYDNY 65
           T  T LL +L+ FL    + A ++ + D            N  G RWA+L AGS GY NY
Sbjct: 5   TATTSLLALLLLFL-VALVSAGRDLVGDFLRLPSDSGNGDNVHGTRWAILFAGSSGYWNY 64

Query: 66  RHQADVCHAYQILKKGGIPDENIIVFMYDDIALDPNNPRQGFIINKPGGPDVYQGVPKDY 125
           RHQAD+CHAYQ+L+KGG+ DENIIVFMYDDIA +  NPR+G IIN P G +VY+GVPKDY
Sbjct: 65  RHQADICHAYQLLRKGGLKDENIIVFMYDDIAFNSENPRRGVIINSPNGDEVYKGVPKDY 124

Query: 126 TGKHVNAVNFYAAILGNKSALTGGSGKVIHSGPHDHIFIYYTDHGAAGMLGMPEGDYVFV 185
           TG+ V A NFYAA+LG+KS LTGGSGKV++SGP+DHIFI+Y+DHG  G+LG P G Y++ 
Sbjct: 125 TGEDVTAHNFYAALLGDKSKLTGGSGKVVNSGPNDHIFIFYSDHGGPGVLGSPAGPYIYA 184

Query: 186 KDLIDVLKRKHEANSYKSMVIYVEACESGSMFEGQLPENIKIYATTASNATEDSWATYCP 245
            DL +VLK+KH + +YK++V Y+EACESGS+FEG LPE+I +YATTASNA E SW TYCP
Sbjct: 185 SDLNEVLKKKHASGTYKNLVFYLEACESGSIFEGLLPEDINVYATTASNADESSWGTYCP 244

Query: 246 KESRSPPPKGYNTCLGDLYSIAWMEDSDKHDSSKETLIQQYHAVRRRTMVDKSGYGSHVM 305
            E  SPPP+ Y+TCLGDLYS+AWMEDSD+H+   ETL QQY  V+ RT+     YGSHVM
Sbjct: 245 GEDPSPPPE-YSTCLGDLYSVAWMEDSDRHNLRTETLHQQYKLVKERTISGGLYYGSHVM 304

Query: 306 LYGNKRIGNNSLNTYIGNSPDYNNYKSSVKSTQTISSPSKLSFNVVSQRDASLIYYQYKF 365
            YG+  +  + L  Y+G  P   N   +     ++ S SK     V+QRDA L+++  KF
Sbjct: 305 QYGDVGLSKDILFHYLGTDPANENL--TFVDENSLWSSSK----AVNQRDADLVHFWDKF 364

Query: 366 QQAPFGSREKMEARKQLEDEIMKRRHADNSINNIGRILFGQARSSKVLNKVREQGQSLVD 425
           ++AP GS +K EARKQ+ + +  R H D+S+  +G++LFG  ++ ++LN VR  G +LVD
Sbjct: 365 RKAPEGSPKKNEARKQVLEVMSHRMHIDDSVELVGKLLFGIEKAPELLNAVRPAGSALVD 424

Query: 426 DWDCFKTFVKIYEKHCERMSSYGMKYARAIANICNAGITMHQMDQACLQTCL 469
           DWDC KT V+ +E HC  +S YGMK+ R+ AN+CN GI   QM +A  Q C+
Sbjct: 425 DWDCLKTMVRTFETHCGSLSQYGMKHMRSFANMCNVGIKKEQMREASAQACV 468

BLAST of Cla97C06G118420 vs. ExPASy Swiss-Prot
Match: P49044 (Vacuolar-processing enzyme OS=Vicia sativa OX=3908 PE=1 SV=1)

HSP 1 Score: 506.9 bits (1304), Expect = 2.4e-142
Identity = 247/430 (57.44%), Postives = 316/430 (73.49%), Query Frame = 0

Query: 40  GKRWALLIAGSYGYDNYRHQADVCHAYQILKKGGIPDENIIVFMYDDIALDPNNPRQGFI 99
           G RWA+L+AGS GY NYRHQ+DVCHAYQ+L+KGG  +ENIIVFMYDDIA +  NPR G I
Sbjct: 54  GTRWAILLAGSNGYWNYRHQSDVCHAYQLLRKGGSKEENIIVFMYDDIASNEENPRPGVI 113

Query: 100 INKPGGPDVYQGVPKDYTGKHVNAVNFYAAILGNKSALTGGSGKVIHSGPHDHIFIYYTD 159
           INKP G DVY GVPKDYTG  V+A NFYAA+LGNKSALTGGSGKV+ SGP+DHIF+YYTD
Sbjct: 114 INKPDGDDVYAGVPKDYTGAEVHADNFYAALLGNKSALTGGSGKVVDSGPNDHIFVYYTD 173

Query: 160 HGAAGMLGMPEGDYVFVKDLIDVLKRKHEANSYKSMVIYVEACESGSMFEGQLPENIKIY 219
           HG  G+LGMP G Y++  DL +VLK+KH + +YKS+V Y+EACESGS+FEG LP+++ IY
Sbjct: 174 HGGPGVLGMPVGPYLYASDLNEVLKKKHASGTYKSLVFYLEACESGSIFEGLLPDDLNIY 233

Query: 220 ATTASNATEDSWATYCPKESRSPPPKGYNTCLGDLYSIAWMEDSDKHDSSKETLIQQYHA 279
           ATTASNA E SW  YCP + + PPP  Y+TCLGDLYSIAWMEDS+ H+   E+L QQY  
Sbjct: 234 ATTASNAEESSWGYYCPGD-KPPPPPEYSTCLGDLYSIAWMEDSEVHNLQTESLQQQYKL 293

Query: 280 VRRRTMVDKSGYGSHVMLYGNKRIGNNSLNTYIGNSP--DYNNYKSSVKSTQTISSPSKL 339
           V+ RT+ +   YGSHVM YG+  +  N L  Y+G +P  D N++    +++  + +PS  
Sbjct: 294 VKNRTISEP--YGSHVMEYGDIGLSKNDLYQYLGTNPANDNNSFVDETENSLKLRTPSA- 353

Query: 340 SFNVVSQRDASLIYYQYKFQQAPFGSREKMEARKQLEDEIMKRRHADNSINNIGRILFGQ 399
               V+QRDA LI++  KF++AP GS +K EA KQ+ + +  R+H DNS+  IG++LFG 
Sbjct: 354 ---AVNQRDADLIHFWEKFRKAPEGSSQKNEAEKQVLEAMSHRKHIDNSVKLIGQLLFGI 413

Query: 400 ARSSKVLNKVREQGQSLVDDWDCFKTFVKIYEKHCERMSSYGMKYARAIANICNAGITMH 459
            + +++L+ VR  G  LVD+WDC KT VK +E HC  +S YGMK+ R+ ANICNAGI   
Sbjct: 414 EKGTELLDVVRPAGSPLVDNWDCLKTMVKTFETHCGSLSQYGMKHMRSFANICNAGIPNE 473

Query: 460 QMDQACLQTC 468
            M +A  Q C
Sbjct: 474 PMAEASAQAC 476

BLAST of Cla97C06G118420 vs. ExPASy Swiss-Prot
Match: P49047 (Vacuolar-processing enzyme alpha-isozyme OS=Arabidopsis thaliana OX=3702 GN=At2g25940 PE=2 SV=2)

HSP 1 Score: 497.3 bits (1279), Expect = 1.9e-139
Identity = 241/426 (56.57%), Postives = 309/426 (72.54%), Query Frame = 0

Query: 42  RWALLIAGSYGYDNYRHQADVCHAYQILKKGGIPDENIIVFMYDDIALDPNNPRQGFIIN 101
           +WA+L+AGS GY NYRHQADVCHAYQ+LKKGG+ +ENI+VFMYDDIA +  NPR G IIN
Sbjct: 44  KWAVLVAGSSGYWNYRHQADVCHAYQLLKKGGVKEENIVVFMYDDIAKNEENPRPGVIIN 103

Query: 102 KPGGPDVYQGVPKDYTGKHVNAVNFYAAILGNKSALTGGSGKVIHSGPHDHIFIYYTDHG 161
            P G DVY GVPKDYTG  VN  N  A ILGNK+AL GGSGKV+ SGP+DHIFIYY+DHG
Sbjct: 104 SPNGEDVYNGVPKDYTGDEVNVDNLLAVILGNKTALKGGSGKVVDSGPNDHIFIYYSDHG 163

Query: 162 AAGMLGMPEGDYVFVKDLIDVLKRKHEANSYKSMVIYVEACESGSMFEGQLPENIKIYAT 221
             G+LGMP    ++  DL DVLK+K+ + +YKS+V Y+EACESGS+FEG LPE + IYAT
Sbjct: 164 GPGVLGMPTSPNLYANDLNDVLKKKYASGTYKSLVFYLEACESGSIFEGLLPEGLNIYAT 223

Query: 222 TASNATEDSWATYCPKESRSPPPKGYNTCLGDLYSIAWMEDSDKHDSSKETLIQQYHAVR 281
           TASNA E SW TYCP E  SPP + Y TCLGDLYS+AW+EDS+KH+   ETL +QY  V+
Sbjct: 224 TASNAEESSWGTYCPGEDPSPPSE-YETCLGDLYSVAWIEDSEKHNLQTETLHEQYELVK 283

Query: 282 RRTMVDKSGYGSHVMLYGNKRIGNNSLNTYIGNSPDYNNYKSSVKSTQTISSPSKLSFNV 341
           +RT      YGSHVM +G+  +    L  ++G +P   N+  +  +  +I  PS+    V
Sbjct: 284 KRTAGSGKSYGSHVMEFGDIGLSKEKLVLFMGTNPADENF--TFVNENSIRPPSR----V 343

Query: 342 VSQRDASLIYYQYKFQQAPFGSREKMEARKQLEDEIMKRRHADNSINNIGRILFGQARSS 401
            +QRDA L+++ +K+Q+AP GS  K+EA+KQ+ + +  R H DNSI  IG +LFG     
Sbjct: 344 TNQRDADLVHFWHKYQKAPEGSARKVEAQKQVLEAMSHRLHVDNSILLIGILLFG-LEGH 403

Query: 402 KVLNKVREQGQSLVDDWDCFKTFVKIYEKHCERMSSYGMKYARAIANICNAGITMHQMDQ 461
            VLNKVR  G+ LVDDWDC K+ V+ +E+HC  +S YG+K+ R+IAN+CNAGI M QM++
Sbjct: 404 AVLNKVRPSGEPLVDDWDCLKSLVRAFERHCGSLSQYGIKHMRSIANMCNAGIQMRQMEE 461

Query: 462 ACLQTC 468
           A +Q C
Sbjct: 464 AAMQAC 461

BLAST of Cla97C06G118420 vs. ExPASy Swiss-Prot
Match: P49043 (Vacuolar-processing enzyme OS=Citrus sinensis OX=2711 PE=2 SV=1)

HSP 1 Score: 496.9 bits (1278), Expect = 2.5e-139
Identity = 240/431 (55.68%), Postives = 309/431 (71.69%), Query Frame = 0

Query: 37  NQTGKRWALLIAGSYGYDNYRHQADVCHAYQILKKGGIPDENIIVFMYDDIALDPNNPRQ 96
           +  G RWA+L+AGS G+ NYRHQAD+CHAYQ+L+KGG+ DENIIVFMYDDIA +  NPR 
Sbjct: 55  DSVGTRWAVLLAGSNGFWNYRHQADICHAYQLLRKGGLKDENIIVFMYDDIAFNEENPRP 114

Query: 97  GFIINKPGGPDVYQGVPKDYTGKHVNAVNFYAAILGNKSALTGGSGKVIHSGPHDHIFIY 156
           G IIN P G DVY+GVPKDYTG+ V    F+A +LGNK+ALTGGSGKV+ SGP+DHIFI+
Sbjct: 115 GVIINHPHGDDVYKGVPKDYTGEDVTVEKFFAVVLGNKTALTGGSGKVVDSGPNDHIFIF 174

Query: 157 YTDHGAAGMLGMPEGDYVFVKDLIDVLKRKHEANSYKSMVIYVEACESGSMFEGQLPENI 216
           Y+DHG  G+LGMP   Y++  +LIDVLK+KH + +YKS+V Y+EACESGS+FEG L E +
Sbjct: 175 YSDHGGPGVLGMPTSRYIYADELIDVLKKKHASGNYKSLVFYLEACESGSIFEGLLLEGL 234

Query: 217 KIYATTASNATEDSWATYCPKESRSPPPKGYNTCLGDLYSIAWMEDSDKHDSSKETLIQQ 276
            IYATTASNA E SW TYCP E   PPP+ Y+TCLGDLYSIAWMEDSD H+   ETL QQ
Sbjct: 235 NIYATTASNAEESSWGTYCPGEIPGPPPE-YSTCLGDLYSIAWMEDSDIHNLRTETLHQQ 294

Query: 277 YHAVRRRTMVDKSGYGSHVMLYGNKRIGNNSLNTYIGNSPDYNNYKSSVKSTQTISSPSK 336
           Y  V+ RT    + YGSHVM YG+  +  N+L TY+G +P  +NY      T    +  +
Sbjct: 295 YELVKTRT-ASYNSYGSHVMQYGDIGLSKNNLFTYLGTNPANDNY------TFVDENSLR 354

Query: 337 LSFNVVSQRDASLIYYQYKFQQAPFGSREKMEARKQLEDEIMKRRHADNSINNIGRILFG 396
            +   V+QRDA L+++  K+++AP G+  K EA+KQ  + +  R H D+SI  IG++LFG
Sbjct: 355 PASKAVNQRDADLLHFWDKYRKAPEGTPRKAEAQKQFFEAMSHRMHVDHSIKLIGKLLFG 414

Query: 397 QARSSKVLNKVREQGQSLVDDWDCFKTFVKIYEKHCERMSSYGMKYARAIANICNAGITM 456
             +  ++LN VR  GQ LVDDW C K+ V+ +E HC  +S YGMK+ R++ANICN GI  
Sbjct: 415 IEKGPEILNTVRPAGQPLVDDWGCLKSLVRTFESHCGALSQYGMKHMRSLANICNTGIGK 474

Query: 457 HQMDQACLQTC 468
            +M +A  Q C
Sbjct: 475 EKMAEASAQAC 477

BLAST of Cla97C06G118420 vs. ExPASy TrEMBL
Match: A0A5A7TXA7 (Vacuolar-processing enzyme gamma-isozyme-like OS=Cucumis melo var. makuwa OX=1194695 GN=E6C27_scaffold114G00680 PE=3 SV=1)

HSP 1 Score: 807.4 bits (2084), Expect = 3.2e-230
Identity = 399/471 (84.71%), Postives = 423/471 (89.81%), Query Frame = 0

Query: 1   MTTCDTNPTKLLIILITFLFFNNIIAKKEWISDATTNQTGKRWALLIAGSYGYDNYRHQA 60
           MTTCD NPTKLLIILITFL FNNIIAK EWISDATT+QTGK WALLIAGS GYDNYRHQ 
Sbjct: 1   MTTCDRNPTKLLIILITFLCFNNIIAKNEWISDATTDQTGKIWALLIAGSNGYDNYRHQ- 60

Query: 61  DVCHAYQILKKGGIPDENIIVFMYDDIALDPNNPRQGFIINKPGGPDVYQGVPKDYTGKH 120
                      GGIPDENIIVFMYDDIA  P+NPR G IINKP G DVY GVPKDYTGKH
Sbjct: 61  -----------GGIPDENIIVFMYDDIAFHPSNPRPGIIINKPDGNDVYHGVPKDYTGKH 120

Query: 121 VNAVNFYAAILGNKSALTGGSGKVIHSGPHDHIFIYYTDHGAAGMLGMPEGDYVFVKDLI 180
           VNAVNFYAAILGNKSALTGGSGKVIHSGP DHIFIYY+DHGAAGMLGMPEGDY+FV DLI
Sbjct: 121 VNAVNFYAAILGNKSALTGGSGKVIHSGPQDHIFIYYSDHGAAGMLGMPEGDYIFVNDLI 180

Query: 181 DVLKRKHEANSYKSMVIYVEACESGSMFEGQLPENIKIYATTASNATEDSWATYCPKESR 240
           DVLKRKH+A +YKSMVIYVEACESGSMFEG LPENIKIYATTASNATEDSWATYCPK+S 
Sbjct: 181 DVLKRKHKAKNYKSMVIYVEACESGSMFEGVLPENIKIYATTASNATEDSWATYCPKQSH 240

Query: 241 SPPPKGYNTCLGDLYSIAWMEDSDKHDSSKETLIQQYHAVRRRTMVDKSGYGSHVMLYGN 300
              PK YNTCLGDL+S+AWMEDSDKHD SKETL QQYHAVRRRT VDKSGYGSHVMLYGN
Sbjct: 241 R-SPKDYNTCLGDLFSVAWMEDSDKHDLSKETLNQQYHAVRRRTFVDKSGYGSHVMLYGN 300

Query: 301 KRIGNNSLNTYIGNSPDYNNYKSSVKSTQTISSPSKLSFNVVSQRDASLIYYQYKFQQAP 360
           KRIGN+ L+T++GNSPDY+NYK SV+S QTISS SKLS N VSQRDASLI+YQYKFQ+AP
Sbjct: 301 KRIGNDFLDTFLGNSPDYDNYKYSVQSPQTISSLSKLSSNSVSQRDASLIHYQYKFQEAP 360

Query: 361 FGSREKMEARKQLEDEIMKRRHADNSINNIGRILFGQARSSKVLNKVREQGQSLVDDWDC 420
           FGSREKMEAR+QLEDEI KRRHADNSIN+IG++LFGQARSS++L+ VR QGQSLVDDW C
Sbjct: 361 FGSREKMEAREQLEDEIFKRRHADNSINSIGKLLFGQARSSEILHNVRAQGQSLVDDWHC 420

Query: 421 FKTFVKIYEKHCERMSSYGMKYARAIANICNAGITMHQMDQACLQTCLGKT 472
           FKTFVKIYEKHC RMSSYGMKY RAIANICNAGITMHQMDQACLQTCLGKT
Sbjct: 421 FKTFVKIYEKHCNRMSSYGMKYTRAIANICNAGITMHQMDQACLQTCLGKT 458

BLAST of Cla97C06G118420 vs. ExPASy TrEMBL
Match: A0A5D3DZP1 (Vacuolar-processing enzyme gamma-isozyme-like OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold403G00260 PE=3 SV=1)

HSP 1 Score: 780.4 bits (2014), Expect = 4.2e-222
Identity = 391/471 (83.01%), Postives = 413/471 (87.69%), Query Frame = 0

Query: 1   MTTCDTNPTKLLIILITFLFFNNIIAKKEWISDATTNQTGKRWALLIAGSYGYDNYRHQA 60
           MTTCD NPTKLLIILITFL FNNIIAK EWISDATT+QTGK WALLIAGS GYDNYRHQ 
Sbjct: 1   MTTCDRNPTKLLIILITFLCFNNIIAKNEWISDATTDQTGKIWALLIAGSNGYDNYRHQ- 60

Query: 61  DVCHAYQILKKGGIPDENIIVFMYDDIALDPNNPRQGFIINKPGGPDVYQGVPKDYTGKH 120
                      GGIPDENIIVFMYDDIA  P+NPR G IINKP G DVY GVPKDYTGKH
Sbjct: 61  -----------GGIPDENIIVFMYDDIAFHPSNPRPGIIINKPDGNDVYHGVPKDYTGKH 120

Query: 121 VNAVNFYAAILGNKSALTGGSGKVIHSGPHDHIFIYYTDHGAAGMLGMPEGDYVFVKDLI 180
           VNAVNFYAAILGNKSALTGGSGKVIHSGP DHIFIYY+DHGAAGMLGMPEGDY+FV DLI
Sbjct: 121 VNAVNFYAAILGNKSALTGGSGKVIHSGPQDHIFIYYSDHGAAGMLGMPEGDYIFVNDLI 180

Query: 181 DVLKRKHEANSYKSMVIYVEACESGSMFEGQLPENIKIYATTASNATEDSWATYCPKESR 240
           DVLKRKH+A +YKSMVIYVEACESGSMFEG LPENIKIYATTASNATEDSWATYCPK+S 
Sbjct: 181 DVLKRKHKAKNYKSMVIYVEACESGSMFEGVLPENIKIYATTASNATEDSWATYCPKQSH 240

Query: 241 SPPPKGYNTCLGDLYSIAWMEDSDKHDSSKETLIQQYHAVRRRTMVDKSGYGSHVMLYGN 300
              PK YNTCLGD          DKHD SKETL QQYHAVRRRT VDKSGYGSHVMLYGN
Sbjct: 241 R-SPKDYNTCLGDF---------DKHDLSKETLNQQYHAVRRRTFVDKSGYGSHVMLYGN 300

Query: 301 KRIGNNSLNTYIGNSPDYNNYKSSVKSTQTISSPSKLSFNVVSQRDASLIYYQYKFQQAP 360
           KRIGN+ L+T++GNSPDY+NYK SV+S QTISS SKLS N VSQRDASLI+YQYKFQ+AP
Sbjct: 301 KRIGNDFLDTFLGNSPDYDNYKYSVQSPQTISSLSKLSSNSVSQRDASLIHYQYKFQEAP 360

Query: 361 FGSREKMEARKQLEDEIMKRRHADNSINNIGRILFGQARSSKVLNKVREQGQSLVDDWDC 420
           FGSREKMEAR+QLEDEI KRRHADNSIN+IG++LFGQARSS++L+ VR QGQSLVDDW C
Sbjct: 361 FGSREKMEAREQLEDEIFKRRHADNSINSIGKLLFGQARSSEILHNVRAQGQSLVDDWHC 420

Query: 421 FKTFVKIYEKHCERMSSYGMKYARAIANICNAGITMHQMDQACLQTCLGKT 472
           FKTFVKIYEKHC RMSSYGMKY RAIANICNAGITMHQMDQACLQTCLGKT
Sbjct: 421 FKTFVKIYEKHCNRMSSYGMKYTRAIANICNAGITMHQMDQACLQTCLGKT 449

BLAST of Cla97C06G118420 vs. ExPASy TrEMBL
Match: A0A6J1HBF5 (vacuolar-processing enzyme gamma-isozyme-like OS=Cucurbita moschata OX=3662 GN=LOC111461846 PE=3 SV=1)

HSP 1 Score: 741.1 bits (1912), Expect = 2.8e-210
Identity = 363/471 (77.07%), Postives = 407/471 (86.41%), Query Frame = 0

Query: 1   MTTCDTNPTKLLIILITFLFFNNIIAKKEWISDATTNQTGKRWALLIAGSYGYDNYRHQA 60
           MTT  T PT LL  LI  LFFN+IIAKK+W+S A T+Q GKRWALL+AGS GYDNYRHQA
Sbjct: 19  MTTFATTPTTLL--LIALLFFNSIIAKKQWVSHANTDQPGKRWALLVAGSNGYDNYRHQA 78

Query: 61  DVCHAYQILKKGGIPDENIIVFMYDDIALDPNNPRQGFIINKPGGPDVYQGVPKDYTGKH 120
           DVCHAYQIL+K GIPDENIIVFMYDDIAL P+NPR G IINKPGG DVYQGVPKDYTGK 
Sbjct: 79  DVCHAYQILRK-GIPDENIIVFMYDDIALHPDNPRPGIIINKPGGADVYQGVPKDYTGKD 138

Query: 121 VNAVNFYAAILGNKSALTGGSGKVIHSGPHDHIFIYYTDHGAAGMLGMPEGDYVFVKDLI 180
           VN+VNFYAAILGNKS +TGGSGKVI SGP+DHIFIYYTDHGAAGMLGMP GDY++  DL+
Sbjct: 139 VNSVNFYAAILGNKSGVTGGSGKVIDSGPNDHIFIYYTDHGAAGMLGMPMGDYIYSNDLM 198

Query: 181 DVLKRKHEANSYKSMVIYVEACESGSMFEGQLPENIKIYATTASNATEDSWATYCPKESR 240
           DVLKRKHEA SYKSMVIYVEACE+GS+FEG LPEN+ IYATTASNATEDSWA YCP +S 
Sbjct: 199 DVLKRKHEAKSYKSMVIYVEACEAGSLFEGLLPENMNIYATTASNATEDSWAAYCPGQSP 258

Query: 241 SPPPKGYNTCLGDLYSIAWMEDSDKHDSSKETLIQQYHAVRRRTMVDKSGYGSHVMLYGN 300
           + P  GY+TCLGDLYS+AWMEDSDKHDSSKETL QQYH VRRRT VDK GYGSHVMLYGN
Sbjct: 259 T-PHTGYDTCLGDLYSVAWMEDSDKHDSSKETLNQQYHTVRRRTSVDKVGYGSHVMLYGN 318

Query: 301 KRIGNNSLNTYIGNSPDYNNYKSSVKSTQTISSPSKLSFNVVSQRDASLIYYQYKFQQAP 360
           + IGNN LNTY GN+ D NN +SSVKST  ISS S    N VSQRDASL++Y YKFQ AP
Sbjct: 319 ESIGNNFLNTYFGNTSDDNNNESSVKSTHIISSLS----NGVSQRDASLLHYWYKFQNAP 378

Query: 361 FGSREKMEARKQLEDEIMKRRHADNSINNIGRILFGQARSSKVLNKVREQGQSLVDDWDC 420
           +GSREKMEA ++LEDEI+ RRHADNS+N IG +LFG+A+SS+VLN++R  GQS VDDW+C
Sbjct: 379 YGSREKMEASERLEDEILSRRHADNSVNQIGELLFGEAKSSRVLNRIRGHGQSFVDDWNC 438

Query: 421 FKTFVKIYEKHCERMSSYGMKYARAIANICNAGITMHQMDQACLQTCLGKT 472
           FK +VKIYEKHC+RMS YGMKY +A+ANICNAGIT+ QMD+ACLQTC+G T
Sbjct: 439 FKKYVKIYEKHCKRMSRYGMKYTQALANICNAGITIDQMDKACLQTCVGNT 481

BLAST of Cla97C06G118420 vs. ExPASy TrEMBL
Match: A0A6J1D475 (vacuolar-processing enzyme gamma-isozyme-like isoform X2 OS=Momordica charantia OX=3673 GN=LOC111016825 PE=3 SV=1)

HSP 1 Score: 740.0 bits (1909), Expect = 6.3e-210
Identity = 359/472 (76.06%), Postives = 411/472 (87.08%), Query Frame = 0

Query: 1   MTTCDTNPTKLLIILITFLFFNNIIAKKEWISDATTNQTGKRWALLIAGSYGYDNYRHQA 60
           M TC    TK  ++LI  L F++IIAK+E +  A+T+Q GKRWA+L+AGS GY+NYRHQA
Sbjct: 1   MATCYATSTK-FVLLIALLLFSDIIAKRESVDGASTDQPGKRWAILVAGSSGYENYRHQA 60

Query: 61  DVCHAYQILKKGGIPDENIIVFMYDDIALDPNNPRQGFIINKPGGPDVYQGVPKDYTGKH 120
           DVCHAYQIL+KGG+PDENIIVFMYDDIA +P+NPR G +INKP G DVYQGVPKDYTG+H
Sbjct: 61  DVCHAYQILRKGGLPDENIIVFMYDDIAFNPSNPRPGVVINKPDGVDVYQGVPKDYTGEH 120

Query: 121 VNAVNFYAAILGNKSALTGGSGKVIHSGPHDHIFIYYTDHGAAGMLGMPEGDYVFVKDLI 180
           VN++NFYA ILGN+SALTGGSGKV+ S  HDHIFIYYTDHG+AG+LGMPEGDYV+ KDL+
Sbjct: 121 VNSINFYAVILGNRSALTGGSGKVVDSDLHDHIFIYYTDHGSAGLLGMPEGDYVYAKDLM 180

Query: 181 DVLKRKHEANSYKSMVIYVEACESGSMFEGQLPENIKIYATTASNATEDSWATYCPKESR 240
           +VLK+KHEA SYKSMVIYVEACESGSM EG LPENIKIYATTASNATE+SWATYCP +  
Sbjct: 181 EVLKQKHEAKSYKSMVIYVEACESGSMLEGLLPENIKIYATTASNATENSWATYCPGQFP 240

Query: 241 SPPPKGYNTCLGDLYSIAWMEDSDKHDSSKETLIQQYHAVRRRTMVDKSGYGSHVMLYGN 300
           S PP  Y+TCLGDLYSIAWMEDSDKHD SKETLIQQY AVRRRT+VDK GYGSHVMLYGN
Sbjct: 241 S-PPTDYDTCLGDLYSIAWMEDSDKHDLSKETLIQQYDAVRRRTLVDKFGYGSHVMLYGN 300

Query: 301 KRIGNNSLNTYIGNSPDYNNYKSSVKSTQTISSPSKLSF-NVVSQRDASLIYYQYKFQQA 360
           K IGNNSL+TYIG +PD  NY SSV+ST TI+ PSKL + N VSQRDASLI+Y +KFQ+A
Sbjct: 301 KSIGNNSLDTYIGANPDNYNYTSSVQSTDTIAPPSKLLYSNAVSQRDASLIHYWHKFQKA 360

Query: 361 PFGSREKMEARKQLEDEIMKRRHADNSINNIGRILFGQARSSKVLNKVREQGQSLVDDWD 420
           PFGSREK EARKQLEDEI+ RRH D+SI +I ++LFGQA+SS+VLN VR+QGQSLVDDW 
Sbjct: 361 PFGSREKTEARKQLEDEILNRRHVDSSIYHIAKLLFGQAKSSEVLNNVRQQGQSLVDDWG 420

Query: 421 CFKTFVKIYEKHCERMSSYGMKYARAIANICNAGITMHQMDQACLQTCLGKT 472
           CFK FVK YEKHC R+S YGMKY RA+ANICNAGIT++QMDQACL+TCL KT
Sbjct: 421 CFKKFVKTYEKHCRRLSRYGMKYTRALANICNAGITINQMDQACLETCLVKT 470

BLAST of Cla97C06G118420 vs. ExPASy TrEMBL
Match: A0A6J1JF37 (vacuolar-processing enzyme gamma-isozyme-like OS=Cucurbita maxima OX=3661 GN=LOC111485183 PE=3 SV=1)

HSP 1 Score: 738.0 bits (1904), Expect = 2.4e-209
Identity = 365/471 (77.49%), Postives = 401/471 (85.14%), Query Frame = 0

Query: 1   MTTCDTNPTKLLIILITFLFFNNIIAKKEWISDATTNQTGKRWALLIAGSYGYDNYRHQA 60
           MTT  T PT LL  LI  LFFN+IIAKKEW+S A T+Q GKRWALL+AGS GYDNYRHQA
Sbjct: 1   MTTFATTPTTLL--LIPLLFFNSIIAKKEWVSHANTDQPGKRWALLVAGSNGYDNYRHQA 60

Query: 61  DVCHAYQILKKGGIPDENIIVFMYDDIALDPNNPRQGFIINKPGGPDVYQGVPKDYTGKH 120
           DVCHAYQIL+K GIPDENIIVFMYDDIA  P+NPR G IINKPGG DVY GVPKDYTGK 
Sbjct: 61  DVCHAYQILRK-GIPDENIIVFMYDDIAFHPDNPRPGIIINKPGGADVYHGVPKDYTGKD 120

Query: 121 VNAVNFYAAILGNKSALTGGSGKVIHSGPHDHIFIYYTDHGAAGMLGMPEGDYVFVKDLI 180
           VN+VNFYAAILGNKS +TGGSGKVI SGP+DHIFIYYTDHGAAGMLGMP GDY++  DL+
Sbjct: 121 VNSVNFYAAILGNKSGVTGGSGKVIDSGPNDHIFIYYTDHGAAGMLGMPMGDYIYSNDLM 180

Query: 181 DVLKRKHEANSYKSMVIYVEACESGSMFEGQLPENIKIYATTASNATEDSWATYCPKESR 240
           DVLKRKHEA SYKSMVIYVEACESGSMFEG LPEN+ IYATTASNATEDSWA YCP +S 
Sbjct: 181 DVLKRKHEAKSYKSMVIYVEACESGSMFEGLLPENMNIYATTASNATEDSWAAYCPGQSP 240

Query: 241 SPPPKGYNTCLGDLYSIAWMEDSDKHDSSKETLIQQYHAVRRRTMVDKSGYGSHVMLYGN 300
           + P  GY+TCLGDLYS+AWMEDSDKHDSSKETL QQYH VRRRT VDK GYGSHVMLYGN
Sbjct: 241 T-PHTGYDTCLGDLYSVAWMEDSDKHDSSKETLNQQYHTVRRRTSVDKVGYGSHVMLYGN 300

Query: 301 KRIGNNSLNTYIGNSPDYNNYKSSVKSTQTISSPSKLSFNVVSQRDASLIYYQYKFQQAP 360
           +  GNN LNTY GN+ D NN +SSVKST  ISS S    N VSQRDASL++Y YKFQ AP
Sbjct: 301 ESFGNNFLNTYFGNTSDDNNNESSVKSTHIISSLS----NAVSQRDASLLHYWYKFQNAP 360

Query: 361 FGSREKMEARKQLEDEIMKRRHADNSINNIGRILFGQARSSKVLNKVREQGQSLVDDWDC 420
           +GSREKMEA +QLEDEI+ RRHADNSIN IG +LFG+A+SS+VL + R  GQS VDDW+C
Sbjct: 361 YGSREKMEASEQLEDEILSRRHADNSINQIGDLLFGEAKSSRVLYRSRGHGQSFVDDWNC 420

Query: 421 FKTFVKIYEKHCERMSSYGMKYARAIANICNAGITMHQMDQACLQTCLGKT 472
           FK +VKIYEKHC+RMS YGMKY +A+AN CNAGITM QMDQACLQTC+G T
Sbjct: 421 FKNYVKIYEKHCKRMSRYGMKYTQALANTCNAGITMDQMDQACLQTCVGNT 463

BLAST of Cla97C06G118420 vs. TAIR 10
Match: AT4G32940.1 (gamma vacuolar processing enzyme )

HSP 1 Score: 510.4 bits (1313), Expect = 1.6e-144
Identity = 245/431 (56.84%), Postives = 315/431 (73.09%), Query Frame = 0

Query: 37  NQTGKRWALLIAGSYGYDNYRHQADVCHAYQILKKGGIPDENIIVFMYDDIALDPNNPRQ 96
           + +G RWA+L+AGS GY NYRHQAD+CHAYQ+L+KGG+ +ENI+VFMYDDIA +  NPR 
Sbjct: 54  SNSGTRWAVLVAGSSGYWNYRHQADICHAYQLLRKGGLKEENIVVFMYDDIANNYENPRP 113

Query: 97  GFIINKPGGPDVYQGVPKDYTGKHVNAVNFYAAILGNKSALTGGSGKVIHSGPHDHIFIY 156
           G IIN P G DVYQGVPKDYTG  VN  N +A ILG+K+A+ GGSGKV+ SGP+DHIFI+
Sbjct: 114 GTIINSPHGKDVYQGVPKDYTGDDVNVDNLFAVILGDKTAVKGGSGKVVDSGPNDHIFIF 173

Query: 157 YTDHGAAGMLGMPEGDYVFVKDLIDVLKRKHEANSYKSMVIYVEACESGSMFEGQLPENI 216
           Y+DHG  G+LGMP   Y++  DL DVLK+KH   +YKS+V Y+EACESGS+FEG LPE +
Sbjct: 174 YSDHGGPGVLGMPTSPYLYANDLNDVLKKKHALGTYKSLVFYLEACESGSIFEGLLPEGL 233

Query: 217 KIYATTASNATEDSWATYCPKESRSPPPKGYNTCLGDLYSIAWMEDSDKHDSSKETLIQQ 276
            IYATTASNA E SW TYCP E  SPPP+ Y TCLGDLYS+AWMEDS  H+   ETL QQ
Sbjct: 234 NIYATTASNAEESSWGTYCPGEEPSPPPE-YETCLGDLYSVAWMEDSGMHNLQTETLHQQ 293

Query: 277 YHAVRRRTMVDKSGYGSHVMLYGNKRIGNNSLNTYIGNSPDYNNYKSSVKSTQTISSPSK 336
           Y  V+RRT      YGSHVM YG+  I  ++L+ Y+G +P  +N+  +     ++  PS+
Sbjct: 294 YELVKRRTAPVGYSYGSHVMQYGDVGISKDNLDLYMGTNPANDNF--TFADANSLKPPSR 353

Query: 337 LSFNVVSQRDASLIYYQYKFQQAPFGSREKMEARKQLEDEIMKRRHADNSINNIGRILFG 396
               V +QRDA L+++  K+++AP GS  K EA+KQ+ + +  R H DNS+  +G+ILFG
Sbjct: 354 ----VTNQRDADLVHFWEKYRKAPEGSARKTEAQKQVLEAMSHRLHIDNSVILVGKILFG 413

Query: 397 QARSSKVLNKVREQGQSLVDDWDCFKTFVKIYEKHCERMSSYGMKYARAIANICNAGITM 456
            +R  +VLNKVR  GQ LVDDW+C K  V+ +E+HC  +S YG+K+ R+ ANICNAGI M
Sbjct: 414 ISRGPEVLNKVRSAGQPLVDDWNCLKNQVRAFERHCGSLSQYGIKHMRSFANICNAGIQM 473

Query: 457 HQMDQACLQTC 468
            QM++A  Q C
Sbjct: 474 EQMEEAASQAC 477

BLAST of Cla97C06G118420 vs. TAIR 10
Match: AT2G25940.1 (alpha-vacuolar processing enzyme )

HSP 1 Score: 497.3 bits (1279), Expect = 1.4e-140
Identity = 241/426 (56.57%), Postives = 309/426 (72.54%), Query Frame = 0

Query: 42  RWALLIAGSYGYDNYRHQADVCHAYQILKKGGIPDENIIVFMYDDIALDPNNPRQGFIIN 101
           +WA+L+AGS GY NYRHQADVCHAYQ+LKKGG+ +ENI+VFMYDDIA +  NPR G IIN
Sbjct: 44  KWAVLVAGSSGYWNYRHQADVCHAYQLLKKGGVKEENIVVFMYDDIAKNEENPRPGVIIN 103

Query: 102 KPGGPDVYQGVPKDYTGKHVNAVNFYAAILGNKSALTGGSGKVIHSGPHDHIFIYYTDHG 161
            P G DVY GVPKDYTG  VN  N  A ILGNK+AL GGSGKV+ SGP+DHIFIYY+DHG
Sbjct: 104 SPNGEDVYNGVPKDYTGDEVNVDNLLAVILGNKTALKGGSGKVVDSGPNDHIFIYYSDHG 163

Query: 162 AAGMLGMPEGDYVFVKDLIDVLKRKHEANSYKSMVIYVEACESGSMFEGQLPENIKIYAT 221
             G+LGMP    ++  DL DVLK+K+ + +YKS+V Y+EACESGS+FEG LPE + IYAT
Sbjct: 164 GPGVLGMPTSPNLYANDLNDVLKKKYASGTYKSLVFYLEACESGSIFEGLLPEGLNIYAT 223

Query: 222 TASNATEDSWATYCPKESRSPPPKGYNTCLGDLYSIAWMEDSDKHDSSKETLIQQYHAVR 281
           TASNA E SW TYCP E  SPP + Y TCLGDLYS+AW+EDS+KH+   ETL +QY  V+
Sbjct: 224 TASNAEESSWGTYCPGEDPSPPSE-YETCLGDLYSVAWIEDSEKHNLQTETLHEQYELVK 283

Query: 282 RRTMVDKSGYGSHVMLYGNKRIGNNSLNTYIGNSPDYNNYKSSVKSTQTISSPSKLSFNV 341
           +RT      YGSHVM +G+  +    L  ++G +P   N+  +  +  +I  PS+    V
Sbjct: 284 KRTAGSGKSYGSHVMEFGDIGLSKEKLVLFMGTNPADENF--TFVNENSIRPPSR----V 343

Query: 342 VSQRDASLIYYQYKFQQAPFGSREKMEARKQLEDEIMKRRHADNSINNIGRILFGQARSS 401
            +QRDA L+++ +K+Q+AP GS  K+EA+KQ+ + +  R H DNSI  IG +LFG     
Sbjct: 344 TNQRDADLVHFWHKYQKAPEGSARKVEAQKQVLEAMSHRLHVDNSILLIGILLFG-LEGH 403

Query: 402 KVLNKVREQGQSLVDDWDCFKTFVKIYEKHCERMSSYGMKYARAIANICNAGITMHQMDQ 461
            VLNKVR  G+ LVDDWDC K+ V+ +E+HC  +S YG+K+ R+IAN+CNAGI M QM++
Sbjct: 404 AVLNKVRPSGEPLVDDWDCLKSLVRAFERHCGSLSQYGIKHMRSIANMCNAGIQMRQMEE 461

Query: 462 ACLQTC 468
           A +Q C
Sbjct: 464 AAMQAC 461

BLAST of Cla97C06G118420 vs. TAIR 10
Match: AT3G20210.1 (delta vacuolar processing enzyme )

HSP 1 Score: 467.2 bits (1201), Expect = 1.5e-131
Identity = 240/463 (51.84%), Postives = 306/463 (66.09%), Query Frame = 0

Query: 10  KLLIILITFLFFNNIIAKKEWISD-----ATTNQTGKRWALLIAGSYGYDNYRHQADVCH 69
           ++L+ L   L F+    K + ++D     +  +  G RWA+L+AGS  Y NYRHQAD+CH
Sbjct: 9   QILVFLHALLIFSAESRKTQLLNDNDVESSDKSAKGTRWAVLVAGSNEYYNYRHQADICH 68

Query: 70  AYQILKKGGIPDENIIVFMYDDIALDPNNPRQGFIINKPGGPDVYQGVPKDYTGKHVNAV 129
           AYQIL+KGG+ DENIIVFMYDDIA    NPR G IINKP G DVY+GVPKDYT + VN  
Sbjct: 69  AYQILRKGGLKDENIIVFMYDDIAFSSENPRPGVIINKPDGEDVYKGVPKDYTKEAVNVQ 128

Query: 130 NFYAAILGNKSALTGGSGKVIHSGPHDHIFIYYTDHGAAGMLGMPEGDYVFVKDLIDVLK 189
           NFY  +LGN+S +TGG+GKV+ SGP+D+IFIYY DHGA G++ MP GD V  KD  +VL+
Sbjct: 129 NFYNVLLGNESGVTGGNGKVVKSGPNDNIFIYYADHGAPGLIAMPTGDEVMAKDFNEVLE 188

Query: 190 RKHEANSYKSMVIYVEACESGSMFEGQLPENIKIYATTASNATEDSWATYCPKESRSPPP 249
           + H+   Y  MVIYVEACESGSMFEG L +N+ IYA TA+N+ E SW  YCP ES  PPP
Sbjct: 189 KMHKRKKYNKMVIYVEACESGSMFEGILKKNLNIYAVTAANSKESSWGVYCP-ESYPPPP 248

Query: 250 KGYNTCLGDLYSIAWMEDSDKHDSSKETLIQQYHAVRRRTMVDKSGYGSHVMLYGNKRIG 309
               TCLGD +SI+W+EDSD HD SKETL QQYH V+RR   D     SHV  +G +++ 
Sbjct: 249 SEIGTCLGDTFSISWLEDSDLHDMSKETLEQQYHVVKRRVGSDVP-ETSHVCRFGTEKML 308

Query: 310 NNSLNTYIGNSPDYNNYKSSVKSTQTISSPSKLSFNVVSQRDASLIYYQYKFQQAPFGSR 369
            + L++YIG +P+ +N+      T++ SSP   S  +V+ RD  L+Y Q K Q+AP GS 
Sbjct: 309 KDYLSSYIGRNPENDNF----TFTESFSSPISNS-GLVNPRDIPLLYLQRKIQKAPMGSL 368

Query: 370 EKMEARKQLEDEIMKRRHADNSINNIGRILFGQARSSKVLNKVREQGQSLVDDWDCFKTF 429
           E  EA+K+L DE   R+  D SI +I R+   Q     +L   R  GQ LVDDWDCFKT 
Sbjct: 369 ESKEAQKKLLDEKNHRKQIDQSITDILRLSVKQTNVLNLLTSTRTTGQPLVDDWDCFKTL 428

Query: 430 VKIYEKHCERMSSYGMKYARAIANICNAGITMHQMDQACLQTC 468
           V  ++ HC     YG+KY  A+ANICN G+ + Q   A  Q C
Sbjct: 429 VNSFKNHCGATVHYGLKYTGALANICNMGVDVKQTVSAIEQAC 464

BLAST of Cla97C06G118420 vs. TAIR 10
Match: AT3G20210.2 (delta vacuolar processing enzyme )

HSP 1 Score: 464.2 bits (1193), Expect = 1.3e-130
Identity = 239/461 (51.84%), Postives = 305/461 (66.16%), Query Frame = 0

Query: 10  KLLIILITFLFFNNIIAKKEWISD-----ATTNQTGKRWALLIAGSYGYDNYRHQADVCH 69
           ++L+ L   L F+    K + ++D     +  +  G RWA+L+AGS  Y NYRHQAD+CH
Sbjct: 9   QILVFLHALLIFSAESRKTQLLNDNDVESSDKSAKGTRWAVLVAGSNEYYNYRHQADICH 68

Query: 70  AYQILKKGGIPDENIIVFMYDDIALDPNNPRQGFIINKPGGPDVYQGVPKDYTGKHVNAV 129
           AYQIL+KGG+ DENIIVFMYDDIA    NPR G IINKP G DVY+GVPKDYT + VN  
Sbjct: 69  AYQILRKGGLKDENIIVFMYDDIAFSSENPRPGVIINKPDGEDVYKGVPKDYTKEAVNVQ 128

Query: 130 NFYAAILGNKSALTGGSGKVIHSGPHDHIFIYYTDHGAAGMLGMPEGDYVFVKDLIDVLK 189
           NFY  +LGN+S +TGG+GKV+ SGP+D+IFIYY DHGA G++ MP GD V  KD  +VL+
Sbjct: 129 NFYNVLLGNESGVTGGNGKVVKSGPNDNIFIYYADHGAPGLIAMPTGDEVMAKDFNEVLE 188

Query: 190 RKHEANSYKSMVIYVEACESGSMFEGQLPENIKIYATTASNATEDSWATYCPKESRSPPP 249
           + H+   Y  MVIYVEACESGSMFEG L +N+ IYA TA+N+ E SW  YCP ES  PPP
Sbjct: 189 KMHKRKKYNKMVIYVEACESGSMFEGILKKNLNIYAVTAANSKESSWGVYCP-ESYPPPP 248

Query: 250 KGYNTCLGDLYSIAWMEDSDKHDSSKETLIQQYHAVRRRTMVDKSGYGSHVMLYGNKRIG 309
               TCLGD +SI+W+EDSD HD SKETL QQYH V+RR   D     SHV  +G +++ 
Sbjct: 249 SEIGTCLGDTFSISWLEDSDLHDMSKETLEQQYHVVKRRVGSDVP-ETSHVCRFGTEKML 308

Query: 310 NNSLNTYIGNSPDYNNYKSSVKSTQTISSPSKLSFNVVSQRDASLIYYQYKFQQAPFGSR 369
            + L++YIG +P+ +N+      T++ SSP   S  +V+ RD  L+Y Q K Q+AP GS 
Sbjct: 309 KDYLSSYIGRNPENDNF----TFTESFSSPISNS-GLVNPRDIPLLYLQRKIQKAPMGSL 368

Query: 370 EKMEARKQLEDEIMKRRHADNSINNIGRILFGQARSSKVLNKVREQGQSLVDDWDCFKTF 429
           E  EA+K+L DE   R+  D SI +I R+   Q     +L   R  GQ LVDDWDCFKT 
Sbjct: 369 ESKEAQKKLLDEKNHRKQIDQSITDILRLSVKQTNVLNLLTSTRTTGQPLVDDWDCFKTL 428

Query: 430 VKIYEKHCERMSSYGMKYARAIANICNAGITMHQMDQACLQ 466
           V  ++ HC     YG+KY  A+ANICN G+ + Q   A  Q
Sbjct: 429 VNSFKNHCGATVHYGLKYTGALANICNMGVDVKQTVSAIEQ 462

BLAST of Cla97C06G118420 vs. TAIR 10
Match: AT1G62710.1 (beta vacuolar processing enzyme )

HSP 1 Score: 461.8 bits (1187), Expect = 6.4e-130
Identity = 229/439 (52.16%), Postives = 296/439 (67.43%), Query Frame = 0

Query: 32  SDATTNQTGKRWALLIAGSYGYDNYRHQADVCHAYQILKKGGIPDENIIVFMYDDIALDP 91
           +D   +  G RWA+L+AGS GY NYRHQADVCHAYQIL+KGG+ +ENI+V MYDDIA  P
Sbjct: 41  ADQDEDGVGTRWAVLVAGSSGYGNYRHQADVCHAYQILRKGGLKEENIVVLMYDDIANHP 100

Query: 92  NNPRQGFIINKPGGPDVYQGVPKDYTGKHVNAVNFYAAILGNKSALTGGSGKVIHSGPHD 151
            NPR G +IN P G DVY GVPKDYTG  V A NFYA +LG++ A+ GGSGKVI S P+D
Sbjct: 101 LNPRPGTLINHPDGDDVYAGVPKDYTGSSVTAANFYAVLLGDQKAVKGGSGKVIASKPND 160

Query: 152 HIFIYYTDHGAAGMLGMPEGDYVFVKDLIDVLKRKHEANSYKSMVIYVEACESGSMFEGQ 211
           HIF+YY DHG  G+LGMP   +++  D I+ LK+KH + +YK MVIYVEACESGS+FEG 
Sbjct: 161 HIFVYYADHGGPGVLGMPNTPHIYAADFIETLKKKHASGTYKEMVIYVEACESGSIFEGI 220

Query: 212 LPENIKIYATTASNATEDSWATYCPKESRSPPPKGYNTCLGDLYSIAWMEDSDKHDSSKE 271
           +P+++ IY TTASNA E S+ TYCP  + SPP + Y TCLGDLYS+AWMEDS+ H+  KE
Sbjct: 221 MPKDLNIYVTTASNAQESSYGTYCPGMNPSPPSE-YITCLGDLYSVAWMEDSETHNLKKE 280

Query: 272 TLIQQYHAVRRRTM-VDKSGYGSHVMLYGNKRIGNNSLNTYIGNSPDYNNYKSSVKSTQT 331
           T+ QQYH V+ RT   +    GSHVM YGN  I +  L  Y G  P   N   +      
Sbjct: 281 TIKQQYHTVKMRTSNYNTYSGGSHVMEYGNNSIKSEKLYLYQGFDPATVNLPLN------ 340

Query: 332 ISSPSKLSFNVVSQRDASLIYYQYKFQQAPFGSREKMEARKQLEDEIMKRRHADNSINNI 391
              P K    VV+QRDA L++  + ++ +  GSR+K +  K+L +    R+H D S+  I
Sbjct: 341 -ELPVKSKIGVVNQRDADLLFLWHMYRTSEDGSRKKDDTLKELTETTRHRKHLDASVELI 400

Query: 392 GRILFGQARSSKVLNKVREQGQSLVDDWDCFKTFVKIYEKHCERMSSYGMKYARAIANIC 451
             ILFG   +  VLN VRE G  LVDDW+C K+ V+++E+HC  ++ YGMK+ RA AN+C
Sbjct: 401 ATILFGP--TMNVLNLVREPGLPLVDDWECLKSMVRVFEEHCGSLTQYGMKHMRAFANVC 460

Query: 452 NAGITMHQMDQACLQTCLG 470
           N G++   M++A    C G
Sbjct: 461 NNGVSKELMEEASTAACGG 469

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
XP_038880044.12.0e-25089.60vacuolar-processing enzyme-like [Benincasa hispida][more]
KAA0046547.16.7e-23084.71vacuolar-processing enzyme gamma-isozyme-like [Cucumis melo var. makuwa][more]
TYK28710.18.7e-22283.01vacuolar-processing enzyme gamma-isozyme-like [Cucumis melo var. makuwa][more]
XP_023516171.11.8e-21177.92vacuolar-processing enzyme gamma-isozyme-like [Cucurbita pepo subsp. pepo][more]
XP_022961313.15.9e-21077.07vacuolar-processing enzyme gamma-isozyme-like [Cucurbita moschata][more]
Match NameE-valueIdentityDescription
Q391192.2e-14356.84Vacuolar-processing enzyme gamma-isozyme OS=Arabidopsis thaliana OX=3702 GN=At4g... [more]
O243251.9e-14253.60Vacuolar-processing enzyme OS=Phaseolus vulgaris OX=3885 PE=1 SV=1[more]
P490442.4e-14257.44Vacuolar-processing enzyme OS=Vicia sativa OX=3908 PE=1 SV=1[more]
P490471.9e-13956.57Vacuolar-processing enzyme alpha-isozyme OS=Arabidopsis thaliana OX=3702 GN=At2g... [more]
P490432.5e-13955.68Vacuolar-processing enzyme OS=Citrus sinensis OX=2711 PE=2 SV=1[more]
Match NameE-valueIdentityDescription
A0A5A7TXA73.2e-23084.71Vacuolar-processing enzyme gamma-isozyme-like OS=Cucumis melo var. makuwa OX=119... [more]
A0A5D3DZP14.2e-22283.01Vacuolar-processing enzyme gamma-isozyme-like OS=Cucumis melo var. makuwa OX=119... [more]
A0A6J1HBF52.8e-21077.07vacuolar-processing enzyme gamma-isozyme-like OS=Cucurbita moschata OX=3662 GN=L... [more]
A0A6J1D4756.3e-21076.06vacuolar-processing enzyme gamma-isozyme-like isoform X2 OS=Momordica charantia ... [more]
A0A6J1JF372.4e-20977.49vacuolar-processing enzyme gamma-isozyme-like OS=Cucurbita maxima OX=3661 GN=LOC... [more]
Match NameE-valueIdentityDescription
AT4G32940.11.6e-14456.84gamma vacuolar processing enzyme [more]
AT2G25940.11.4e-14056.57alpha-vacuolar processing enzyme [more]
AT3G20210.11.5e-13151.84delta vacuolar processing enzyme [more]
AT3G20210.21.3e-13051.84delta vacuolar processing enzyme [more]
AT1G62710.16.4e-13052.16beta vacuolar processing enzyme [more]
InterPro
Analysis Name: InterPro Annotations of Watermelon (97103) v2.5
Date Performed: 2022-01-31
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR001096Peptidase C13, legumainPRINTSPR00776HEMOGLOBNASEcoord: 43..67
score: 69.91
coord: 68..97
score: 57.97
coord: 143..162
score: 60.65
coord: 115..130
score: 50.27
coord: 192..208
score: 67.26
IPR001096Peptidase C13, legumainPFAMPF01650Peptidase_C13coord: 42..312
e-value: 8.7E-107
score: 356.4
IPR001096Peptidase C13, legumainPIRSFPIRSF019663Legumaincoord: 5..471
e-value: 6.0E-201
score: 666.3
IPR001096Peptidase C13, legumainPANTHERPTHR12000HEMOGLOBINASE FAMILY MEMBERcoord: 28..468
NoneNo IPR availableGENE3D3.40.50.1460coord: 40..337
e-value: 3.4E-120
score: 402.8
NoneNo IPR availableGENE3D1.10.132.130coord: 344..471
e-value: 5.7E-35
score: 122.1
NoneNo IPR availablePIRSRPIRSR019663-1PIRSR019663-1coord: 40..469
e-value: 4.0E-192
score: 637.1
NoneNo IPR availablePANTHERPTHR12000:SF45VACUOLAR-PROCESSING ENZYME-LIKEcoord: 28..468
IPR043577Asparaginyl endopeptidasePIRSFPIRSF500139AEcoord: 8..471
e-value: 1.5E-202
score: 671.8

Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
Cla97C06G118420.1Cla97C06G118420.1mRNA


GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
biological_process GO:0051603 proteolysis involved in cellular protein catabolic process
biological_process GO:0006508 proteolysis
cellular_component GO:0016021 integral component of membrane
molecular_function GO:0004197 cysteine-type endopeptidase activity
molecular_function GO:0008233 peptidase activity