Cla97C06G109880 (gene) Watermelon (97103) v2.5

BLAST of Cla97C06G109880 vs. NCBI nr
Match: XP_038876325.1 (LOW QUALITY PROTEIN: protein NUCLEAR FUSION DEFECTIVE 4-like [Benincasa hispida])

HSP 1 Score: 1097.8 bits (2838), Expect = 0.0e+00
Identity = 566/600 (94.33%), Postives = 579/600 (96.50%), Query Frame = 0

Query: 48  MAYRPNHSPNAFFNHYSPQASAMKIKQRKAPQIPLLPIKKQKRMLNQPGQWVVLVAAIWI 107
           MAYRPNHSPNAFFNHYSPQASAMKIKQRK  Q+PLLPIKK KRM+NQPG+WVVLVAAIWI
Sbjct: 1   MAYRPNHSPNAFFNHYSPQASAMKIKQRKTQQVPLLPIKKHKRMVNQPGKWVVLVAAIWI 60

Query: 108 QAFAGTNFDFPSYSSDLKAALGMSQVELNYLAVASDLGKAFGWCSGVALLYFPLWVVMFM 167
           QAFAGTNFDFPSYSSDLKAALGMSQVELNYLAVASDLGKAFGWCSGVALLYFPLWVVMFM
Sbjct: 61  QAFAGTNFDFPSYSSDLKAALGMSQVELNYLAVASDLGKAFGWCSGVALLYFPLWVVMFM 120

Query: 168 AASMGFLGYGLQWLLLQRIVYLPYSMVYLLCLMAGCSICWFNTVCYVSCIQNFPANRALA 227
           AASMGFLGYGLQWLLLQRIV LPYSMVYLLCLMAGCSICWFNTVCYVSCIQNFPANRALA
Sbjct: 121 AASMGFLGYGLQWLLLQRIVSLPYSMVYLLCLMAGCSICWFNTVCYVSCIQNFPANRALA 180

Query: 228 LSLIVSFNGVSAALYTLIANAVDPNDAGLYLFLNALVPLIISIVALLPILHQPSVQPSSA 287
           LSLIVSFNGVSAALYTLIANA+DPNDA LYLFLNALVPLIIS+VA LPILHQP VQPSSA
Sbjct: 181 LSLIVSFNGVSAALYTLIANAIDPNDASLYLFLNALVPLIISVVAFLPILHQPPVQPSSA 240

Query: 288 DAIRHDSLIFICLYITAVITGVYLITFNSKPSYKYRSQILLAGALALLLVPLCLPGILST 347
           DAIRHDSLIFICLYITA+ITG+YLI+FNS PS KY SQILLAGA ALLLVPLCLPGILST
Sbjct: 241 DAIRHDSLIFICLYITAIITGLYLISFNSMPSNKYGSQILLAGAFALLLVPLCLPGILST 300

Query: 348 RRWLARIISTSLSRLIHSRFNLVDHELHQELIAVGTERNGMKVIIPFDSKEKESTSRKVM 407
           RRWL RIISTSLS LIHSRFNLVDHELHQELIAVGTERNGM VI+PFDS EKES SRKVM
Sbjct: 301 RRWLVRIISTSLSCLIHSRFNLVDHELHQELIAVGTERNGMTVIVPFDSNEKESISRKVM 360

Query: 408 EKENLVMLEEEHSAKMLLRRLDFWLYYVAYFCGGTIGLVYSNNLGQIAQSLGYSSSTSSL 467
           EKENLV+LEEEHSAKML+RRLDFWLYYVAYFCGGTIGLVYSNNLGQIAQSLGYSSSTSSL
Sbjct: 361 EKENLVVLEEEHSAKMLMRRLDFWLYYVAYFCGGTIGLVYSNNLGQIAQSLGYSSSTSSL 420

Query: 468 VTLYSSCSFFGRLISAAPDFMREKVRFARTGWLAIALVPTPIAFILLAASGSKIALQAGT 527
           VTLYSSCSFFGRLISAAPDFMREKV FARTGWLAIALVPTPIAFILLAAS SKIALQAGT
Sbjct: 421 VTLYSSCSFFGRLISAAPDFMREKVHFARTGWLAIALVPTPIAFILLAASDSKIALQAGT 480

Query: 528 GLIGLSSGFIFSASVSITSELFGPNSSGVNHNILITNIPLGSFLYGVLAAVAYDSNAESS 587
           GLIGL SGFIFSASVSITSELFGPNSSGVNHNILITNIPLGSFLYGVLAAVAYDSNA SS
Sbjct: 481 GLIGLCSGFIFSASVSITSELFGPNSSGVNHNILITNIPLGSFLYGVLAAVAYDSNAGSS 540

Query: 588 HQMAALGDAVVCIGQNCYLQTFVWWACISIFGLASSFLLFRRTRPAYDRHYESNLSKMQP 647
           HQ AALGDAVVCIGQNCYLQTFVWWACISIFGLASSFLLFRRT+PAYDRHYESN+SKMQP
Sbjct: 541 HQTAALGDAVVCIGQNCYLQTFVWWACISIFGLASSFLLFRRTKPAYDRHYESNVSKMQP 600

BLAST of Cla97C06G109880 vs. NCBI nr
Match: XP_004145067.2 (protein NUCLEAR FUSION DEFECTIVE 4 isoform X1 [Cucumis sativus])

HSP 1 Score: 1040.8 bits (2690), Expect = 4.9e-300
Identity = 533/602 (88.54%), Postives = 566/602 (94.02%), Query Frame = 0

Query: 48  MAYRPNHSP-NAFFNHYSPQASAMKIKQRKAPQIPLLPIKKQKRMLNQPGQWVVLVAAIW 107
           M+Y PNH+P   FFNHYSPQASAMKIKQRK  Q+PLLP KKQ+RM+NQPG+WVVLVAAIW
Sbjct: 1   MSYPPNHAPFRLFFNHYSPQASAMKIKQRKTQQVPLLPFKKQRRMMNQPGKWVVLVAAIW 60

Query: 108 IQAFAGTNFDFPSYSSDLKAALGMSQVELNYLAVASDLGKAFGWCSGVALLYFPLWVVMF 167
           IQAFAGTNFDFPSYSSDLKAALGMSQVELNYLAVASDLGKAFGWCSGVALLYFPLWVVMF
Sbjct: 61  IQAFAGTNFDFPSYSSDLKAALGMSQVELNYLAVASDLGKAFGWCSGVALLYFPLWVVMF 120

Query: 168 MAASMGFLGYGLQWLLLQRIVYLPYSMVYLLCLMAGCSICWFNTVCYVSCIQNFPANRAL 227
           MAASMGFLGYG QWLLLQRI+ LPYSMVYLLCLMAGCSICWFNTVCYVSCIQNFPANRAL
Sbjct: 121 MAASMGFLGYGFQWLLLQRIISLPYSMVYLLCLMAGCSICWFNTVCYVSCIQNFPANRAL 180

Query: 228 ALSLIVSFNGVSAALYTLIANAVDPNDAGLYLFLNALVPLIISIVALLPILHQPSVQPSS 287
           ALSLIVSFNGVSAALYTLIANA+DP+DA LYLFLNALVPLIIS+VALLP+LH+P VQPSS
Sbjct: 181 ALSLIVSFNGVSAALYTLIANAIDPSDASLYLFLNALVPLIISVVALLPMLHKPPVQPSS 240

Query: 288 ADAIRHDSLIFICLYITAVITGVYLITFNSKPSYKYRSQILLAGALALLLVPLCLPGILS 347
            DAIRHDSLIFICLY+TA+ITG+YLITFNS PS KY SQILLAGA ALL+VPLCLPG+LS
Sbjct: 241 DDAIRHDSLIFICLYMTAIITGLYLITFNSMPSNKYGSQILLAGAFALLIVPLCLPGVLS 300

Query: 348 TRRWLARIISTSLSRLIHSRFNLVDHELHQELIAVGTERNGMKVIIPFDSKEKESTSRKV 407
           T RWL RIIST+L+ LIHSRF+LVDHELHQELI + +ERN MK I+PF+SKEKES SRKV
Sbjct: 301 THRWLVRIISTTLNCLIHSRFSLVDHELHQELITIESERNSMKGIVPFESKEKESISRKV 360

Query: 408 MEKENLVMLEEEHSAKMLLRRLDFWLYYVAYFCGGTIGLVYSNNLGQIAQSLGYSSSTSS 467
           MEKENLV+LEEEHSAKML+R+LDFWLYY AYFCGGTIGLVYSN+LGQIAQSLGYSSSTSS
Sbjct: 361 MEKENLVVLEEEHSAKMLMRQLDFWLYYAAYFCGGTIGLVYSNSLGQIAQSLGYSSSTSS 420

Query: 468 LVTLYSSCSFFGRLISAAPDFMREKVRFARTGWLAIALVPTPIAFILLAASGSKIALQAG 527
           LVTLYSSCSFFGRLISAAPDFMRE+  FARTGWLAIALVPTPIAFILLAASGSKIALQ G
Sbjct: 421 LVTLYSSCSFFGRLISAAPDFMRERGHFARTGWLAIALVPTPIAFILLAASGSKIALQVG 480

Query: 528 TGLIGLSSGFIFSASVSITSELFGPNSSGVNHNILITNIPLGSFLYGVLAAVAYDSNAES 587
           T LIGLSSGFIFSASVSITSELFGPNSSGVNHNILITNIPLGSFLYGVLAA+AYDSNA S
Sbjct: 481 TSLIGLSSGFIFSASVSITSELFGPNSSGVNHNILITNIPLGSFLYGVLAAMAYDSNAGS 540

Query: 588 SHQMAALGDAVVCIGQNCYLQTFVWWACISIFGLASSFLLFRRTRPAYDRHYESNLSKMQ 647
           SHQ +ALGDAVVCIGQNCYL TFVWWACISIFGLA SFLLFRRT+ AYDRHY+SN S MQ
Sbjct: 541 SHQTSALGDAVVCIGQNCYLMTFVWWACISIFGLACSFLLFRRTKSAYDRHYDSNPSNMQ 600

Query: 648 PF 649
           PF
Sbjct: 601 PF 602

BLAST of Cla97C06G109880 vs. NCBI nr
Match: XP_008460080.1 (PREDICTED: protein NUCLEAR FUSION DEFECTIVE 4-like [Cucumis melo])

HSP 1 Score: 1027.7 bits (2656), Expect = 4.3e-296
Identity = 531/579 (91.71%), Postives = 549/579 (94.82%), Query Frame = 0

Query: 70  MKIKQRKAPQIPLLPIKKQKRMLNQPGQWVVLVAAIWIQAFAGTNFDFPSYSSDLKAALG 129
           MKIK RK  QIPLLP KKQKRM+NQPG+WVVLVAAIWIQAFAGTNFDFPSYSSDLKAALG
Sbjct: 1   MKIKHRKTQQIPLLPFKKQKRMMNQPGKWVVLVAAIWIQAFAGTNFDFPSYSSDLKAALG 60

Query: 130 MSQVELNYLAVASDLGKAFGWCSGVALLYFPLWVVMFMAASMGFLGYGLQWLLLQRIVYL 189
           MSQVELNYLAVASDLGKAFGWCSGVALLYFPLWVVMFMAASMGFLGYGLQWLLLQRI+ L
Sbjct: 61  MSQVELNYLAVASDLGKAFGWCSGVALLYFPLWVVMFMAASMGFLGYGLQWLLLQRIISL 120

Query: 190 PYSMVYLLCLMAGCSICWFNTVCYVSCIQNFPANRALALSLIVSFNGVSAALYTLIANAV 249
           PYS+VYLLCLMAGCSICWFNTVCYVSCIQNFPANRALALSLIVSFNGVSAALYTLIANA+
Sbjct: 121 PYSVVYLLCLMAGCSICWFNTVCYVSCIQNFPANRALALSLIVSFNGVSAALYTLIANAI 180

Query: 250 DPNDAGLYLFLNALVPLIISIVALLPILHQPSVQPSSADAIRHDSLIFICLYITAVITGV 309
           DP DA LYLFLNALVPLIIS+VALLP+LHQP VQPSSADAIRHDSLIFICLYITAVITG+
Sbjct: 181 DPGDASLYLFLNALVPLIISVVALLPMLHQPQVQPSSADAIRHDSLIFICLYITAVITGL 240

Query: 310 YLITFNSKPSYKYRSQILLAGALALLLVPLCLPGILSTRRWLARIISTSLSRLIHSRFNL 369
           YLITFNS PS KY SQILLAGA ALLLVPLCLPGILST RWL RIISTSL+ LIHSRF+L
Sbjct: 241 YLITFNSMPSNKYGSQILLAGAFALLLVPLCLPGILSTHRWLVRIISTSLNCLIHSRFSL 300

Query: 370 VDHELHQELIAVGTERNGMKVIIPFDSKEKESTSRKVMEKENLVMLEEEHSAKMLLRRLD 429
            DHELHQEL  +G+ERN M  IIPF+SKEKES SRKVMEKENLV+LEEEHSAKML+R+LD
Sbjct: 301 ADHELHQELFTIGSERNSMMDIIPFESKEKESISRKVMEKENLVVLEEEHSAKMLMRQLD 360

Query: 430 FWLYYVAYFCGGTIGLVYSNNLGQIAQSLGYSSSTSSLVTLYSSCSFFGRLISAAPDFMR 489
           FWLYY AYFCGGTIGLVYSNNLGQIAQSLGYSSSTSSLVTLYSSCSFFGRLISAAPDFMR
Sbjct: 361 FWLYYAAYFCGGTIGLVYSNNLGQIAQSLGYSSSTSSLVTLYSSCSFFGRLISAAPDFMR 420

Query: 490 EKVRFARTGWLAIALVPTPIAFILLAASGSKIALQAGTGLIGLSSGFIFSASVSITSELF 549
           EK  FARTGWLAIALVPTPIAF+LLAASGSKIALQAGT LIGLSSGFIFSASVSITSELF
Sbjct: 421 EKGHFARTGWLAIALVPTPIAFVLLAASGSKIALQAGTSLIGLSSGFIFSASVSITSELF 480

Query: 550 GPNSSGVNHNILITNIPLGSFLYGVLAAVAYDSNAESSHQMAALGDAVVCIGQNCYLQTF 609
           GPNSSGVNHNILITNIPLGSFLYGVLAAVAYDSNA SSHQ AALGDAVVCIGQNCYL TF
Sbjct: 481 GPNSSGVNHNILITNIPLGSFLYGVLAAVAYDSNAGSSHQTAALGDAVVCIGQNCYLMTF 540

Query: 610 VWWACISIFGLASSFLLFRRTRPAYDRHYESNLSKMQPF 649
           VWWACISIFGLASSFLLFRRT+ AYDRHYESNLSKMQPF
Sbjct: 541 VWWACISIFGLASSFLLFRRTKSAYDRHYESNLSKMQPF 579

BLAST of Cla97C06G109880 vs. NCBI nr
Match: KAA0039979.1 (protein NUCLEAR FUSION DEFECTIVE 4-like [Cucumis melo var. makuwa] >TYK24523.1 protein NUCLEAR FUSION DEFECTIVE 4-like [Cucumis melo var. makuwa])

HSP 1 Score: 996.1 bits (2574), Expect = 1.4e-286
Identity = 513/558 (91.94%), Postives = 533/558 (95.52%), Query Frame = 0

Query: 91  MLNQPGQWVVLVAAIWIQAFAGTNFDFPSYSSDLKAALGMSQVELNYLAVASDLGKAFGW 150
           M+NQPG+WVVLVAAIWIQAFAGTNFDFPSYSSDLKAALGMSQVELNYLAVASDLGKAFGW
Sbjct: 1   MMNQPGKWVVLVAAIWIQAFAGTNFDFPSYSSDLKAALGMSQVELNYLAVASDLGKAFGW 60

Query: 151 CSGVALLYFPLWVVMFMAASMGFLGYGLQWLLLQRIVYLPYSMVYLLCLMAGCSICWFNT 210
           CSGVALLYFPLWVVMFMAASMGFLGYGLQWLLLQRI+ LPYS+VYLLCLMAGCSICWFNT
Sbjct: 61  CSGVALLYFPLWVVMFMAASMGFLGYGLQWLLLQRIISLPYSVVYLLCLMAGCSICWFNT 120

Query: 211 VCYVSCIQNFPANRALALSLIVSFNGVSAALYTLIANAVDPNDAGLYLFLNALVPLIISI 270
           VCYVSCIQNFPA+RALALSLIVSFNGVSAALYTLIANA+DP DA LYLFLNALVPLIIS+
Sbjct: 121 VCYVSCIQNFPAHRALALSLIVSFNGVSAALYTLIANAIDPGDASLYLFLNALVPLIISV 180

Query: 271 VALLPILHQPSVQPSSADAIRHDSLIFICLYITAVITGVYLITFNSKPSYKYRSQILLAG 330
           VALLP+LHQP VQPSSADAIRHDSLIFICLYITAVITG+YLITFNS PS KY SQILLAG
Sbjct: 181 VALLPMLHQPQVQPSSADAIRHDSLIFICLYITAVITGLYLITFNSMPSNKYGSQILLAG 240

Query: 331 ALALLLVPLCLPGILSTRRWLARIISTSLSRLIHSRFNLVDHELHQELIAVGTERNGMKV 390
           A ALLLVPLCLPGILST RWL RIIST+L+ LIHSRF+L DHELHQELI +G+ERN M  
Sbjct: 241 AFALLLVPLCLPGILSTHRWLVRIISTNLNCLIHSRFSLADHELHQELITIGSERNSMMD 300

Query: 391 IIPFDSKEKESTSRKVMEKENLVMLEEEHSAKMLLRRLDFWLYYVAYFCGGTIGLVYSNN 450
           IIPF+SKEKES SRKVMEKENLV+LEEEHSAKML+R+LDFWLYY AYFCGGTIGLVYSNN
Sbjct: 301 IIPFESKEKESISRKVMEKENLVVLEEEHSAKMLMRQLDFWLYYAAYFCGGTIGLVYSNN 360

Query: 451 LGQIAQSLGYSSSTSSLVTLYSSCSFFGRLISAAPDFMREKVRFARTGWLAIALVPTPIA 510
           LGQIAQSLGYSSSTSSLVTLYSSCSFFGRLISAAPDFMREK  FARTGWLAIALVPTPIA
Sbjct: 361 LGQIAQSLGYSSSTSSLVTLYSSCSFFGRLISAAPDFMREKGHFARTGWLAIALVPTPIA 420

Query: 511 FILLAASGSKIALQAGTGLIGLSSGFIFSASVSITSELFGPNSSGVNHNILITNIPLGSF 570
           F+LLAASGSKIALQAGT LIGLSSGFIFSASVSITSELFGPNSSGVNHNILITNIPLGSF
Sbjct: 421 FVLLAASGSKIALQAGTSLIGLSSGFIFSASVSITSELFGPNSSGVNHNILITNIPLGSF 480

Query: 571 LYGVLAAVAYDSNAESSHQMAALGDAVVCIGQNCYLQTFVWWACISIFGLASSFLLFRRT 630
           LYGVLAAVAYDSNA SSHQ AALGDAVVCIGQNCYL TFVWWACISIFGLASSFLLFRRT
Sbjct: 481 LYGVLAAVAYDSNAGSSHQTAALGDAVVCIGQNCYLMTFVWWACISIFGLASSFLLFRRT 540

Query: 631 RPAYDRHYESNLSKMQPF 649
           + AYDRHYESNLSKMQPF
Sbjct: 541 KSAYDRHYESNLSKMQPF 558

BLAST of Cla97C06G109880 vs. NCBI nr
Match: XP_023515123.1 (protein NUCLEAR FUSION DEFECTIVE 4-like isoform X1 [Cucurbita pepo subsp. pepo])

HSP 1 Score: 944.9 bits (2441), Expect = 3.7e-271
Identity = 489/580 (84.31%), Postives = 524/580 (90.34%), Query Frame = 0

Query: 70  MKIKQRKAPQIPLLPIKKQKRMLNQPGQWVVLVAAIWIQAFAGTNFDFPSYSSDLKAALG 129
           MKIK RK   +PLLP+ KQ+RM+NQPG+WV LVAAIWIQAF GTNFDFPSYSS+LKAALG
Sbjct: 1   MKIKHRKPQNLPLLPVTKQRRMMNQPGKWVALVAAIWIQAFTGTNFDFPSYSSNLKAALG 60

Query: 130 MSQVELNYLAVASDLGKAFGWCSGVALLYFPLWVVMFMAASMGFLGYGLQWLLLQRIVYL 189
           MSQVELNYLAVASD+GKAFGWCSG+ALLYFPLWVVM MAASMGFLGYGLQWLLLQ IV L
Sbjct: 61  MSQVELNYLAVASDVGKAFGWCSGIALLYFPLWVVMLMAASMGFLGYGLQWLLLQGIVSL 120

Query: 190 PYSMVYLLCLMAGCSICWFNTVCYVSCIQNFPANRALALSLIVSFNGVSAALYTLIANAV 249
           PYSMVYLLCL+AGCSICWFNT+CYVSCIQ FPANRALALSLIVSFNGVSAALYTL+ANAV
Sbjct: 121 PYSMVYLLCLLAGCSICWFNTICYVSCIQKFPANRALALSLIVSFNGVSAALYTLMANAV 180

Query: 250 DPNDAGLYLFLNALVPLIISIVALLPILHQPSVQPSSADAIRHDSLIFICLYITAVITGV 309
           DPNDA LYL LNALVPLIIS+V  LPILHQP  QPSS+DA R+DS  FICLYITAVITG+
Sbjct: 181 DPNDASLYLLLNALVPLIISVVTFLPILHQPPAQPSSSDATRNDSHTFICLYITAVITGL 240

Query: 310 YLITFNSKPSYKYRSQILLAGALALLLVPLCLPGILSTRRWLARIISTSLSRLIHSRFNL 369
           YLITFNS PS KY +Q+LLAGA ALLLVPLCLPGI ST +WL RI ST LS ++H RFNL
Sbjct: 241 YLITFNSTPSSKYGAQLLLAGAFALLLVPLCLPGIWSTHKWLFRIASTGLSSVLHFRFNL 300

Query: 370 VDHELHQELIAVGTERNGMKVIIPFDSKEKESTSRKVMEKENLVMLEEEHSAKMLLRRLD 429
           V+HEL QELI + TER+G  VI PFD KEKES SRK+M KENL +LEEEHSAKML+RRLD
Sbjct: 301 VNHELQQELINLETERSGTNVITPFDYKEKESISRKMMGKENLTVLEEEHSAKMLVRRLD 360

Query: 430 FWLYYVAYFCGGTIGLVYSNNLGQIAQSLGYSSSTSSLVTLYSSCSFFGRLISAAPDFMR 489
           FWLY+VAYFCGGTIGLVYSNNLGQI+QSLGYSS TSSLVTLYSSCSFFGRLISA PDFMR
Sbjct: 361 FWLYFVAYFCGGTIGLVYSNNLGQISQSLGYSSLTSSLVTLYSSCSFFGRLISAGPDFMR 420

Query: 490 EKVRFARTGWLAIALVPTPIAFILLAASGSKIALQAGTGLIGLSSGFIFSASVSITSELF 549
           EKV FARTGWLA+ALVPTPIAFILLAASGS+IALQ GTGLIGLSSGF+FSASVSITSELF
Sbjct: 421 EKVHFARTGWLALALVPTPIAFILLAASGSEIALQIGTGLIGLSSGFMFSASVSITSELF 480

Query: 550 GPNSSGVNHNILITNIPLGSFLYGVLAAVAYDSNAESSHQ-MAALGDAVVCIGQNCYLQT 609
           GPNSSGVNHNILITNIPLGSFLYGVLAAV+YDS A SS Q  AALGD VVCIG+ CYLQT
Sbjct: 481 GPNSSGVNHNILITNIPLGSFLYGVLAAVSYDSTAGSSDQTAAALGDVVVCIGRKCYLQT 540

Query: 610 FVWWACISIFGLASSFLLFRRTRPAYDRHYESNLSKMQPF 649
           F+WWACISI GLASSFLLFRRT+PAYDR YESN SK QPF
Sbjct: 541 FIWWACISIVGLASSFLLFRRTKPAYDRQYESNPSKTQPF 580

BLAST of Cla97C06G109880 vs. ExPASy Swiss-Prot
Match: F4I9E1 (Protein NUCLEAR FUSION DEFECTIVE 4 OS=Arabidopsis thaliana OX=3702 GN=NFD4 PE=3 SV=1)

HSP 1 Score: 564.3 bits (1453), Expect = 1.8e-159
Identity = 310/557 (55.66%), Postives = 402/557 (72.17%), Query Frame = 0

Query: 89  KRMLNQPGQWVVLVAAIWIQAFAGTNFDFPSYSSDLKAALGMSQVELNYLAVASDLGKAF 148
           + M  +  +W VLVAAIWIQA  GTNFDF +YSS LK+ LG+SQV LNYLAVASDLGKAF
Sbjct: 35  RTMFGRWRKWTVLVAAIWIQASTGTNFDFSAYSSHLKSVLGISQVRLNYLAVASDLGKAF 94

Query: 149 GWCSGVALLYFPLWVVMFMAASMGFLGYGLQWLLLQRIVYLPYSMVYLLCLMAGCSICWF 208
           GW SG+AL YFPL VV+F AA+MGF+GYG+QWL++  I+ LPYS+V+L CL+AG SICWF
Sbjct: 95  GWSSGIALGYFPLSVVLFAAAAMGFVGYGVQWLVITNIITLPYSLVFLCCLLAGLSICWF 154

Query: 209 NTVCYVSCIQNFPANRALALSLIVSFNGVSAALYTLIANAVDPNDAGLYLFLNALVPLII 268
           NT C++ CI++FP NRALALSL VSFNG+SAALY+L  NA++P+ + LYL LN+LVPL++
Sbjct: 155 NTACFILCIRHFPNNRALALSLTVSFNGISAALYSLAFNAINPSSSNLYLLLNSLVPLVV 214

Query: 269 SIVALLPILHQPSVQPS-SADAIRHDSLIFICLYITAVITGVYLITFNSKPSYKYRSQIL 328
           S  AL P+L +PS+  +   D+ RHDS +F  L + AVIT  +L+  +S  S    +++ 
Sbjct: 215 SFAALYPVLTKPSLDTTPDYDSRRHDSHVFTILNVLAVITSFHLLLSSSSTS---SARLN 274

Query: 329 LAGALALLLVPLCLPGILSTRRWLARIISTSLSRLIHSRFNLVDHELHQELIAVGTERNG 388
             GA+ LL+ PLC P ++  R +   +I+  L+        L   EL  +  +V ++   
Sbjct: 275 FIGAVVLLVFPLCAPLLVYARDYFLPVINARLNHESSGYVMLNIDELKNQKTSVSSK--- 334

Query: 389 MKVIIPFDSKEKESTSRKVMEKENLVMLEEEHSAKMLLRRLDFWLYYVAYFCGGTIGLVY 448
                     E   T++    + N V L +EHS ++L+ RL+FWLYY+AYFCGGTIGLVY
Sbjct: 335 -------TGYEHMGTAK----EGNTVRLGDEHSFRLLISRLEFWLYYIAYFCGGTIGLVY 394

Query: 449 SNNLGQIAQSLGYSSSTSSLVTLYSSCSFFGRLISAAPDFMREKVRFARTGWLAIALVPT 508
           SNNLGQIAQSLG +S+T  LVT+YSS SFFGRL+SAAPDFM ++ R  RTGW AIAL+PT
Sbjct: 395 SNNLGQIAQSLGQNSTT--LVTIYSSFSFFGRLLSAAPDFMHKRFRLTRTGWFAIALLPT 454

Query: 509 PIAFILLAASGS-KIALQAGTGLIGLSSGFIFSASVSITSELFGPNSSGVNHNILITNIP 568
           PIAF LLA S S + ALQ  T LIGLSSGFIF+A+VSITS+LFGPNS GVNHNILITNIP
Sbjct: 455 PIAFFLLAVSSSQQTALQTATALIGLSSGFIFAAAVSITSDLFGPNSVGVNHNILITNIP 514

Query: 569 LGSFLYGVLAAVAYDSNAESSHQMAALGDAVVCIGQNCYLQTFVWWACISIFGLASSFLL 628
           +GS LYG +AA  Y++NA S      + D++VCIG++CY +TFV+W C+SI G+ SS  L
Sbjct: 515 IGSLLYGYIAASIYEANA-SPDITPIVSDSIVCIGRDCYFKTFVFWGCLSILGVVSSLSL 571

Query: 629 FRRTRPAYDRHYESNLS 644
           + RT+P Y R  +  +S
Sbjct: 575 YIRTKPVYHRLEQDKVS 571

BLAST of Cla97C06G109880 vs. ExPASy TrEMBL
Match: A0A0A0KCZ5 (Nodulin-like domain-containing protein OS=Cucumis sativus OX=3659 GN=Csa_6G078620 PE=4 SV=1)

HSP 1 Score: 1040.8 bits (2690), Expect = 2.4e-300
Identity = 533/602 (88.54%), Postives = 566/602 (94.02%), Query Frame = 0

Query: 48  MAYRPNHSP-NAFFNHYSPQASAMKIKQRKAPQIPLLPIKKQKRMLNQPGQWVVLVAAIW 107
           M+Y PNH+P   FFNHYSPQASAMKIKQRK  Q+PLLP KKQ+RM+NQPG+WVVLVAAIW
Sbjct: 1   MSYPPNHAPFRLFFNHYSPQASAMKIKQRKTQQVPLLPFKKQRRMMNQPGKWVVLVAAIW 60

Query: 108 IQAFAGTNFDFPSYSSDLKAALGMSQVELNYLAVASDLGKAFGWCSGVALLYFPLWVVMF 167
           IQAFAGTNFDFPSYSSDLKAALGMSQVELNYLAVASDLGKAFGWCSGVALLYFPLWVVMF
Sbjct: 61  IQAFAGTNFDFPSYSSDLKAALGMSQVELNYLAVASDLGKAFGWCSGVALLYFPLWVVMF 120

Query: 168 MAASMGFLGYGLQWLLLQRIVYLPYSMVYLLCLMAGCSICWFNTVCYVSCIQNFPANRAL 227
           MAASMGFLGYG QWLLLQRI+ LPYSMVYLLCLMAGCSICWFNTVCYVSCIQNFPANRAL
Sbjct: 121 MAASMGFLGYGFQWLLLQRIISLPYSMVYLLCLMAGCSICWFNTVCYVSCIQNFPANRAL 180

Query: 228 ALSLIVSFNGVSAALYTLIANAVDPNDAGLYLFLNALVPLIISIVALLPILHQPSVQPSS 287
           ALSLIVSFNGVSAALYTLIANA+DP+DA LYLFLNALVPLIIS+VALLP+LH+P VQPSS
Sbjct: 181 ALSLIVSFNGVSAALYTLIANAIDPSDASLYLFLNALVPLIISVVALLPMLHKPPVQPSS 240

Query: 288 ADAIRHDSLIFICLYITAVITGVYLITFNSKPSYKYRSQILLAGALALLLVPLCLPGILS 347
            DAIRHDSLIFICLY+TA+ITG+YLITFNS PS KY SQILLAGA ALL+VPLCLPG+LS
Sbjct: 241 DDAIRHDSLIFICLYMTAIITGLYLITFNSMPSNKYGSQILLAGAFALLIVPLCLPGVLS 300

Query: 348 TRRWLARIISTSLSRLIHSRFNLVDHELHQELIAVGTERNGMKVIIPFDSKEKESTSRKV 407
           T RWL RIIST+L+ LIHSRF+LVDHELHQELI + +ERN MK I+PF+SKEKES SRKV
Sbjct: 301 THRWLVRIISTTLNCLIHSRFSLVDHELHQELITIESERNSMKGIVPFESKEKESISRKV 360

Query: 408 MEKENLVMLEEEHSAKMLLRRLDFWLYYVAYFCGGTIGLVYSNNLGQIAQSLGYSSSTSS 467
           MEKENLV+LEEEHSAKML+R+LDFWLYY AYFCGGTIGLVYSN+LGQIAQSLGYSSSTSS
Sbjct: 361 MEKENLVVLEEEHSAKMLMRQLDFWLYYAAYFCGGTIGLVYSNSLGQIAQSLGYSSSTSS 420

Query: 468 LVTLYSSCSFFGRLISAAPDFMREKVRFARTGWLAIALVPTPIAFILLAASGSKIALQAG 527
           LVTLYSSCSFFGRLISAAPDFMRE+  FARTGWLAIALVPTPIAFILLAASGSKIALQ G
Sbjct: 421 LVTLYSSCSFFGRLISAAPDFMRERGHFARTGWLAIALVPTPIAFILLAASGSKIALQVG 480

Query: 528 TGLIGLSSGFIFSASVSITSELFGPNSSGVNHNILITNIPLGSFLYGVLAAVAYDSNAES 587
           T LIGLSSGFIFSASVSITSELFGPNSSGVNHNILITNIPLGSFLYGVLAA+AYDSNA S
Sbjct: 481 TSLIGLSSGFIFSASVSITSELFGPNSSGVNHNILITNIPLGSFLYGVLAAMAYDSNAGS 540

Query: 588 SHQMAALGDAVVCIGQNCYLQTFVWWACISIFGLASSFLLFRRTRPAYDRHYESNLSKMQ 647
           SHQ +ALGDAVVCIGQNCYL TFVWWACISIFGLA SFLLFRRT+ AYDRHY+SN S MQ
Sbjct: 541 SHQTSALGDAVVCIGQNCYLMTFVWWACISIFGLACSFLLFRRTKSAYDRHYDSNPSNMQ 600

Query: 648 PF 649
           PF
Sbjct: 601 PF 602

BLAST of Cla97C06G109880 vs. ExPASy TrEMBL
Match: A0A1S3CCZ1 (protein NUCLEAR FUSION DEFECTIVE 4-like OS=Cucumis melo OX=3656 GN=LOC103499000 PE=4 SV=1)

HSP 1 Score: 1027.7 bits (2656), Expect = 2.1e-296
Identity = 531/579 (91.71%), Postives = 549/579 (94.82%), Query Frame = 0

Query: 70  MKIKQRKAPQIPLLPIKKQKRMLNQPGQWVVLVAAIWIQAFAGTNFDFPSYSSDLKAALG 129
           MKIK RK  QIPLLP KKQKRM+NQPG+WVVLVAAIWIQAFAGTNFDFPSYSSDLKAALG
Sbjct: 1   MKIKHRKTQQIPLLPFKKQKRMMNQPGKWVVLVAAIWIQAFAGTNFDFPSYSSDLKAALG 60

Query: 130 MSQVELNYLAVASDLGKAFGWCSGVALLYFPLWVVMFMAASMGFLGYGLQWLLLQRIVYL 189
           MSQVELNYLAVASDLGKAFGWCSGVALLYFPLWVVMFMAASMGFLGYGLQWLLLQRI+ L
Sbjct: 61  MSQVELNYLAVASDLGKAFGWCSGVALLYFPLWVVMFMAASMGFLGYGLQWLLLQRIISL 120

Query: 190 PYSMVYLLCLMAGCSICWFNTVCYVSCIQNFPANRALALSLIVSFNGVSAALYTLIANAV 249
           PYS+VYLLCLMAGCSICWFNTVCYVSCIQNFPANRALALSLIVSFNGVSAALYTLIANA+
Sbjct: 121 PYSVVYLLCLMAGCSICWFNTVCYVSCIQNFPANRALALSLIVSFNGVSAALYTLIANAI 180

Query: 250 DPNDAGLYLFLNALVPLIISIVALLPILHQPSVQPSSADAIRHDSLIFICLYITAVITGV 309
           DP DA LYLFLNALVPLIIS+VALLP+LHQP VQPSSADAIRHDSLIFICLYITAVITG+
Sbjct: 181 DPGDASLYLFLNALVPLIISVVALLPMLHQPQVQPSSADAIRHDSLIFICLYITAVITGL 240

Query: 310 YLITFNSKPSYKYRSQILLAGALALLLVPLCLPGILSTRRWLARIISTSLSRLIHSRFNL 369
           YLITFNS PS KY SQILLAGA ALLLVPLCLPGILST RWL RIISTSL+ LIHSRF+L
Sbjct: 241 YLITFNSMPSNKYGSQILLAGAFALLLVPLCLPGILSTHRWLVRIISTSLNCLIHSRFSL 300

Query: 370 VDHELHQELIAVGTERNGMKVIIPFDSKEKESTSRKVMEKENLVMLEEEHSAKMLLRRLD 429
            DHELHQEL  +G+ERN M  IIPF+SKEKES SRKVMEKENLV+LEEEHSAKML+R+LD
Sbjct: 301 ADHELHQELFTIGSERNSMMDIIPFESKEKESISRKVMEKENLVVLEEEHSAKMLMRQLD 360

Query: 430 FWLYYVAYFCGGTIGLVYSNNLGQIAQSLGYSSSTSSLVTLYSSCSFFGRLISAAPDFMR 489
           FWLYY AYFCGGTIGLVYSNNLGQIAQSLGYSSSTSSLVTLYSSCSFFGRLISAAPDFMR
Sbjct: 361 FWLYYAAYFCGGTIGLVYSNNLGQIAQSLGYSSSTSSLVTLYSSCSFFGRLISAAPDFMR 420

Query: 490 EKVRFARTGWLAIALVPTPIAFILLAASGSKIALQAGTGLIGLSSGFIFSASVSITSELF 549
           EK  FARTGWLAIALVPTPIAF+LLAASGSKIALQAGT LIGLSSGFIFSASVSITSELF
Sbjct: 421 EKGHFARTGWLAIALVPTPIAFVLLAASGSKIALQAGTSLIGLSSGFIFSASVSITSELF 480

Query: 550 GPNSSGVNHNILITNIPLGSFLYGVLAAVAYDSNAESSHQMAALGDAVVCIGQNCYLQTF 609
           GPNSSGVNHNILITNIPLGSFLYGVLAAVAYDSNA SSHQ AALGDAVVCIGQNCYL TF
Sbjct: 481 GPNSSGVNHNILITNIPLGSFLYGVLAAVAYDSNAGSSHQTAALGDAVVCIGQNCYLMTF 540

Query: 610 VWWACISIFGLASSFLLFRRTRPAYDRHYESNLSKMQPF 649
           VWWACISIFGLASSFLLFRRT+ AYDRHYESNLSKMQPF
Sbjct: 541 VWWACISIFGLASSFLLFRRTKSAYDRHYESNLSKMQPF 579

BLAST of Cla97C06G109880 vs. ExPASy TrEMBL
Match: A0A5A7TFE8 (Protein NUCLEAR FUSION DEFECTIVE 4-like OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold266G00650 PE=4 SV=1)

HSP 1 Score: 996.1 bits (2574), Expect = 6.7e-287
Identity = 513/558 (91.94%), Postives = 533/558 (95.52%), Query Frame = 0

Query: 91  MLNQPGQWVVLVAAIWIQAFAGTNFDFPSYSSDLKAALGMSQVELNYLAVASDLGKAFGW 150
           M+NQPG+WVVLVAAIWIQAFAGTNFDFPSYSSDLKAALGMSQVELNYLAVASDLGKAFGW
Sbjct: 1   MMNQPGKWVVLVAAIWIQAFAGTNFDFPSYSSDLKAALGMSQVELNYLAVASDLGKAFGW 60

Query: 151 CSGVALLYFPLWVVMFMAASMGFLGYGLQWLLLQRIVYLPYSMVYLLCLMAGCSICWFNT 210
           CSGVALLYFPLWVVMFMAASMGFLGYGLQWLLLQRI+ LPYS+VYLLCLMAGCSICWFNT
Sbjct: 61  CSGVALLYFPLWVVMFMAASMGFLGYGLQWLLLQRIISLPYSVVYLLCLMAGCSICWFNT 120

Query: 211 VCYVSCIQNFPANRALALSLIVSFNGVSAALYTLIANAVDPNDAGLYLFLNALVPLIISI 270
           VCYVSCIQNFPA+RALALSLIVSFNGVSAALYTLIANA+DP DA LYLFLNALVPLIIS+
Sbjct: 121 VCYVSCIQNFPAHRALALSLIVSFNGVSAALYTLIANAIDPGDASLYLFLNALVPLIISV 180

Query: 271 VALLPILHQPSVQPSSADAIRHDSLIFICLYITAVITGVYLITFNSKPSYKYRSQILLAG 330
           VALLP+LHQP VQPSSADAIRHDSLIFICLYITAVITG+YLITFNS PS KY SQILLAG
Sbjct: 181 VALLPMLHQPQVQPSSADAIRHDSLIFICLYITAVITGLYLITFNSMPSNKYGSQILLAG 240

Query: 331 ALALLLVPLCLPGILSTRRWLARIISTSLSRLIHSRFNLVDHELHQELIAVGTERNGMKV 390
           A ALLLVPLCLPGILST RWL RIIST+L+ LIHSRF+L DHELHQELI +G+ERN M  
Sbjct: 241 AFALLLVPLCLPGILSTHRWLVRIISTNLNCLIHSRFSLADHELHQELITIGSERNSMMD 300

Query: 391 IIPFDSKEKESTSRKVMEKENLVMLEEEHSAKMLLRRLDFWLYYVAYFCGGTIGLVYSNN 450
           IIPF+SKEKES SRKVMEKENLV+LEEEHSAKML+R+LDFWLYY AYFCGGTIGLVYSNN
Sbjct: 301 IIPFESKEKESISRKVMEKENLVVLEEEHSAKMLMRQLDFWLYYAAYFCGGTIGLVYSNN 360

Query: 451 LGQIAQSLGYSSSTSSLVTLYSSCSFFGRLISAAPDFMREKVRFARTGWLAIALVPTPIA 510
           LGQIAQSLGYSSSTSSLVTLYSSCSFFGRLISAAPDFMREK  FARTGWLAIALVPTPIA
Sbjct: 361 LGQIAQSLGYSSSTSSLVTLYSSCSFFGRLISAAPDFMREKGHFARTGWLAIALVPTPIA 420

Query: 511 FILLAASGSKIALQAGTGLIGLSSGFIFSASVSITSELFGPNSSGVNHNILITNIPLGSF 570
           F+LLAASGSKIALQAGT LIGLSSGFIFSASVSITSELFGPNSSGVNHNILITNIPLGSF
Sbjct: 421 FVLLAASGSKIALQAGTSLIGLSSGFIFSASVSITSELFGPNSSGVNHNILITNIPLGSF 480

Query: 571 LYGVLAAVAYDSNAESSHQMAALGDAVVCIGQNCYLQTFVWWACISIFGLASSFLLFRRT 630
           LYGVLAAVAYDSNA SSHQ AALGDAVVCIGQNCYL TFVWWACISIFGLASSFLLFRRT
Sbjct: 481 LYGVLAAVAYDSNAGSSHQTAALGDAVVCIGQNCYLMTFVWWACISIFGLASSFLLFRRT 540

Query: 631 RPAYDRHYESNLSKMQPF 649
           + AYDRHYESNLSKMQPF
Sbjct: 541 KSAYDRHYESNLSKMQPF 558

BLAST of Cla97C06G109880 vs. ExPASy TrEMBL
Match: A0A6J1HJV4 (protein NUCLEAR FUSION DEFECTIVE 4-like isoform X2 OS=Cucurbita moschata OX=3662 GN=LOC111464237 PE=4 SV=1)

HSP 1 Score: 944.1 bits (2439), Expect = 3.0e-271
Identity = 486/576 (84.38%), Postives = 523/576 (90.80%), Query Frame = 0

Query: 70  MKIKQRKAPQIPLLPIKKQKRMLNQPGQWVVLVAAIWIQAFAGTNFDFPSYSSDLKAALG 129
           MKIK RK   +PLLP+ KQ+RM+NQPG+WV LVAAIWIQAF GTNFDFPSYSS+LKAALG
Sbjct: 1   MKIKHRKPQNLPLLPVTKQRRMMNQPGKWVALVAAIWIQAFTGTNFDFPSYSSNLKAALG 60

Query: 130 MSQVELNYLAVASDLGKAFGWCSGVALLYFPLWVVMFMAASMGFLGYGLQWLLLQRIVYL 189
           MSQVELNYLAVASD+GKAFGWCSG+ALLYFPLWVVM MAASMGFLGYGLQWLLLQ IV L
Sbjct: 61  MSQVELNYLAVASDVGKAFGWCSGIALLYFPLWVVMLMAASMGFLGYGLQWLLLQGIVSL 120

Query: 190 PYSMVYLLCLMAGCSICWFNTVCYVSCIQNFPANRALALSLIVSFNGVSAALYTLIANAV 249
           PYSMVYLLCL+AGCSICWFNT+CYVSCIQ FP NRALALSLIVSFNGVSAALYTL+ANA+
Sbjct: 121 PYSMVYLLCLLAGCSICWFNTICYVSCIQKFPTNRALALSLIVSFNGVSAALYTLMANAL 180

Query: 250 DPNDAGLYLFLNALVPLIISIVALLPILHQPSVQPSSADAIRHDSLIFICLYITAVITGV 309
           DPNDA LYL LNALVPLIIS+V  LPILHQP  QPSSADA R+DSL FICLYITAVITG+
Sbjct: 181 DPNDASLYLLLNALVPLIISVVTFLPILHQPPAQPSSADATRNDSLTFICLYITAVITGL 240

Query: 310 YLITFNSKPSYKYRSQILLAGALALLLVPLCLPGILSTRRWLARIISTSLSRLIHSRFNL 369
           YLITFNS PS KY +Q+LLAGA ALLLVPLCLPGI ST +WL RI ST LS ++H RFNL
Sbjct: 241 YLITFNSTPSSKYGAQLLLAGAFALLLVPLCLPGIWSTHKWLFRIASTGLSSVLHFRFNL 300

Query: 370 VDHELHQELIAVGTERNGMKVIIPFDSKEKESTSRKVMEKENLVMLEEEHSAKMLLRRLD 429
           V+HEL QELI + TER+G KVI PFD KEKES SRK+M KENL +LEEEHSAKML+RRLD
Sbjct: 301 VNHELQQELIPIETERSGTKVITPFDYKEKESISRKMMGKENLTVLEEEHSAKMLIRRLD 360

Query: 430 FWLYYVAYFCGGTIGLVYSNNLGQIAQSLGYSSSTSSLVTLYSSCSFFGRLISAAPDFMR 489
           FWLY+VAYFCGGTIGLVYSNNLGQI+QSLGYSS TSSLVTLYSSCSFFGRLISA PDFMR
Sbjct: 361 FWLYFVAYFCGGTIGLVYSNNLGQISQSLGYSSLTSSLVTLYSSCSFFGRLISAGPDFMR 420

Query: 490 EKVRFARTGWLAIALVPTPIAFILLAASGSKIALQAGTGLIGLSSGFIFSASVSITSELF 549
           EKV FARTGWLA+ALVPTPIAFILLAASGS+IALQ GTGLIGLSSGF+FSASVSITSELF
Sbjct: 421 EKVHFARTGWLALALVPTPIAFILLAASGSEIALQIGTGLIGLSSGFMFSASVSITSELF 480

Query: 550 GPNSSGVNHNILITNIPLGSFLYGVLAAVAYDSNAESSHQ-MAALGDAVVCIGQNCYLQT 609
           GPNSSGVNHNILITNIPLGSFLYGVLAAV+YDS A SS+Q  AALGD VVCIG+ CYLQT
Sbjct: 481 GPNSSGVNHNILITNIPLGSFLYGVLAAVSYDSTAGSSNQTAAALGDVVVCIGRKCYLQT 540

Query: 610 FVWWACISIFGLASSFLLFRRTRPAYDRHYESNLSK 645
           F+WWACISI GLAS FLLFRRT+PAYDRHYESN +K
Sbjct: 541 FIWWACISIIGLASCFLLFRRTKPAYDRHYESNPNK 576

BLAST of Cla97C06G109880 vs. ExPASy TrEMBL
Match: A0A6J1KEQ9 (protein NUCLEAR FUSION DEFECTIVE 4-like isoform X2 OS=Cucurbita maxima OX=3661 GN=LOC111494334 PE=4 SV=1)

HSP 1 Score: 939.5 bits (2427), Expect = 7.5e-270
Identity = 487/580 (83.97%), Postives = 521/580 (89.83%), Query Frame = 0

Query: 70  MKIKQRKAPQIPLLPIKKQKRMLNQPGQWVVLVAAIWIQAFAGTNFDFPSYSSDLKAALG 129
           MKIK RK    P LP+ KQ+RM+NQPG+WV L+AAIWIQAF GTNFDFPSYSS+LKAALG
Sbjct: 1   MKIKHRKPQNFPHLPVTKQRRMMNQPGKWVALMAAIWIQAFTGTNFDFPSYSSNLKAALG 60

Query: 130 MSQVELNYLAVASDLGKAFGWCSGVALLYFPLWVVMFMAASMGFLGYGLQWLLLQRIVYL 189
           MSQVELNYLAVASD+GKAFGWCSG+ALLYFP WVVM MAASMGFLGYGLQWLLLQ IV L
Sbjct: 61  MSQVELNYLAVASDVGKAFGWCSGIALLYFPSWVVMLMAASMGFLGYGLQWLLLQGIVSL 120

Query: 190 PYSMVYLLCLMAGCSICWFNTVCYVSCIQNFPANRALALSLIVSFNGVSAALYTLIANAV 249
           PYSMVYLLCL+AGCSICWFNT+CYVSCIQ FPANRALALSLIVSFNGVSAALYTL+ NAV
Sbjct: 121 PYSMVYLLCLLAGCSICWFNTICYVSCIQKFPANRALALSLIVSFNGVSAALYTLMTNAV 180

Query: 250 DPNDAGLYLFLNALVPLIISIVALLPILHQPSVQPSSADAIRHDSLIFICLYITAVITGV 309
           DPNDA LYL LNALVPLIIS+VA  PILHQP  QPSSADA R+DSL FICLYITAVITG+
Sbjct: 181 DPNDASLYLLLNALVPLIISVVAFFPILHQPPAQPSSADATRNDSLTFICLYITAVITGL 240

Query: 310 YLITFNSKPSYKYRSQILLAGALALLLVPLCLPGILSTRRWLARIISTSLSRLIHSRFNL 369
           YLITFNS PS KY +QILLAGA ALLLVPLCLPGI ST +WL RI ST LS ++H RFNL
Sbjct: 241 YLITFNSTPSSKYGAQILLAGAFALLLVPLCLPGIWSTHKWLFRIASTGLSSVLHFRFNL 300

Query: 370 VDHELHQELIAVGTERNGMKVIIPFDSKEKESTSRKVMEKENLVMLEEEHSAKMLLRRLD 429
           V+HEL QELI V +ER+G KVI P D KEKES SRK+M KENL +LEEEHSAKML+RRLD
Sbjct: 301 VNHELQQELINVESERSGTKVITPCDYKEKESISRKMMGKENLTVLEEEHSAKMLVRRLD 360

Query: 430 FWLYYVAYFCGGTIGLVYSNNLGQIAQSLGYSSSTSSLVTLYSSCSFFGRLISAAPDFMR 489
           FWLY+VAYFCGGTIGLVYSNNLGQI+QSLGYSS TSSLVTLYSSCSFFGRLISA PDFMR
Sbjct: 361 FWLYFVAYFCGGTIGLVYSNNLGQISQSLGYSSLTSSLVTLYSSCSFFGRLISAGPDFMR 420

Query: 490 EKVRFARTGWLAIALVPTPIAFILLAASGSKIALQAGTGLIGLSSGFIFSASVSITSELF 549
           EKV FARTGWLA+ALVP PIAFILLAASGS+IALQ GTGLIGLSSGF+FSASVSITSELF
Sbjct: 421 EKVHFARTGWLALALVPNPIAFILLAASGSEIALQIGTGLIGLSSGFMFSASVSITSELF 480

Query: 550 GPNSSGVNHNILITNIPLGSFLYGVLAAVAYDSNAESSHQMAA-LGDAVVCIGQNCYLQT 609
           GPNSSGVNHNILITNIPLGSFLYGVLAAV+YDS A SS+Q AA LGD VVCIG+ CYLQT
Sbjct: 481 GPNSSGVNHNILITNIPLGSFLYGVLAAVSYDSTAGSSNQTAATLGDVVVCIGRKCYLQT 540

Query: 610 FVWWACISIFGLASSFLLFRRTRPAYDRHYESNLSKMQPF 649
           F+WWACISI GLAS FLLFRRT+PAYDRHYESN SK QPF
Sbjct: 541 FIWWACISIVGLASCFLLFRRTKPAYDRHYESNPSKTQPF 580

BLAST of Cla97C06G109880 vs. TAIR 10
Match: AT4G19450.1 (Major facilitator superfamily protein )

HSP 1 Score: 639.0 bits (1647), Expect = 4.0e-183
Identity = 337/568 (59.33%), Postives = 420/568 (73.94%), Query Frame = 0

Query: 91  MLNQPGQWVVLVAAIWIQAFAGTNFDFPSYSSDLKAALGMSQVELNYLAVASDLGKAFGW 150
           M  Q  +W++LVA IWIQAF GTNFDF +YSSDLK+ LG+SQV+LNYLAVASDLGK FGW
Sbjct: 1   MAGQSRKWMILVATIWIQAFTGTNFDFSAYSSDLKSVLGISQVQLNYLAVASDLGKVFGW 60

Query: 151 CSGVALLYFPLWVVMFMAASMGFLGYGLQWLLLQRIVYLPYSMVYLLCLMAGCSICWFNT 210
            SG+AL+YFPLW V+F AA MGF+GYG+QWL++   + LPY MV+L CL+AG SICWFNT
Sbjct: 61  SSGLALMYFPLWTVLFAAAFMGFVGYGVQWLVITHFISLPYIMVFLCCLLAGLSICWFNT 120

Query: 211 VCYVSCIQNFPANRALALSLIVSFNGVSAALYTLIANAVDPNDAGLYLFLNALVPLIISI 270
           VC+V CI NFPANR+LALSL VSFNGVSAALYTL  NA++P    LYL LNAL+PLI+S 
Sbjct: 121 VCFVLCISNFPANRSLALSLTVSFNGVSAALYTLAYNAINPTSPELYLLLNALIPLIVSF 180

Query: 271 VALLPILHQPSVQPSSADAIRHDSLIFICLYITAVITGVYLITFNSKPSYKYRSQILLAG 330
            A++PIL QP  +P   D +R DSL+F+ L I A + GVYL+ F S  S    +++L  G
Sbjct: 181 TAIIPILRQPPFEPLPPDGVRRDSLMFLLLNILAALNGVYLLLFGSNSSDLTSARLLFGG 240

Query: 331 ALALLLVPLCLPGILSTRRWLARIISTSLSRLIHSRFNLVDH---ELHQELIAVGTERNG 390
           A+ LL+ PLC+PG++  R W  R I TS  RL  S F LVD    ELH+ ++A    R G
Sbjct: 241 AILLLVFPLCIPGLVIARNWYNRTIHTSF-RLEGSGFILVDPDELELHKGMLAHEANREG 300

Query: 391 ------------MKVIIPFDSKEKESTSRKVMEKENLVMLEEEHSAKMLLRRLDFWLYYV 450
                       +K +   +    ES  +K++ ++ L  L  EHS  +LL R DFWLYY+
Sbjct: 301 YQLLSDDVVQNPVKSVAVEEEDSDESCCKKLITRDQLEGLGIEHSLSLLLTRSDFWLYYI 360

Query: 451 AYFCGGTIGLVYSNNLGQIAQSLGYSSSTSSLVTLYSSCSFFGRLISAAPDFMREKVRFA 510
            YFCGGTIGLVYSNNLGQIAQSLG SS+T++LVTLYS+ SFFGRL+SA PD++R KV FA
Sbjct: 361 TYFCGGTIGLVYSNNLGQIAQSLGQSSNTTTLVTLYSAFSFFGRLLSATPDYIRAKVYFA 420

Query: 511 RTGWLAIALVPTPIAFILLAASGSKIALQAGTGLIGLSSGFIFSASVSITSELFGPNSSG 570
           RTGWLAIAL+PTP A  LLA+SG+  ALQAGT L+GLSSGFIF+A+VSITSELFGPNS G
Sbjct: 421 RTGWLAIALLPTPFALFLLASSGTASALQAGTALMGLSSGFIFAAAVSITSELFGPNSVG 480

Query: 571 VNHNILITNIPLGSFLYGVLAAVAYDSNAESSHQMAALGDAVVCIGQNCYLQTFVWWACI 630
           VNHNILITNIP+GS +YG LAA+ YDS+  +  + +   ++VVC+G++CY  TFVWW C+
Sbjct: 481 VNHNILITNIPIGSLIYGFLAALVYDSHGFTGTK-SMTSESVVCMGRDCYYLTFVWWGCL 540

Query: 631 SIFGLASSFLLFRRTRPAYDRHYESNLS 644
           S+ GL SS +LF RTR AY R  ++ +S
Sbjct: 541 SLLGLGSSLVLFIRTRRAYQRFEQARIS 566

BLAST of Cla97C06G109880 vs. TAIR 10
Match: AT5G45275.1 (Major facilitator superfamily protein )

HSP 1 Score: 634.8 bits (1636), Expect = 7.6e-182
Identity = 337/569 (59.23%), Postives = 425/569 (74.69%), Query Frame = 0

Query: 91  MLNQPGQWVVLVAAIWIQAFAGTNFDFPSYSSDLKAALGMSQVELNYLAVASDLGKAFGW 150
           M  Q  +W++LVA IWIQAF GTNFDF +YSS+LK+ LG+SQV+LNYLAVASDLGK FGW
Sbjct: 1   MAGQSRKWMILVATIWIQAFTGTNFDFSTYSSNLKSVLGISQVQLNYLAVASDLGKVFGW 60

Query: 151 CSGVALLYFPLWVVMFMAASMGFLGYGLQWLLLQRIVYLPYSMVYLLCLMAGCSICWFNT 210
            SG+ALLYFPLW V+F AA MGF+GYG+QWL++  ++ LPY +V+L CL+AG SICWFNT
Sbjct: 61  SSGLALLYFPLWTVLFAAAIMGFVGYGVQWLVITNVISLPYILVFLCCLLAGLSICWFNT 120

Query: 211 VCYVSCIQNFPANRALALSLIVSFNGVSAALYTLIANAVDPNDAGLYLFLNALVPLIISI 270
           VC+V CI+NFPANR+LALSL VSFNGVSAALYTL  NA++P    LYL LNALVPL +S 
Sbjct: 121 VCFVLCIRNFPANRSLALSLTVSFNGVSAALYTLAYNAINPVSTELYLLLNALVPLFVSF 180

Query: 271 VALLPILHQPSVQPSSADAIRHDSLIFICLYITAVITGVYLITFNSKPSYKYRSQILLAG 330
            AL+PIL QP ++P   D +R DSL+F+ L I AV+ GVYL+ F SK S    +++L  G
Sbjct: 181 AALIPILRQPPLEPLPPDGVRRDSLMFLLLNILAVLNGVYLLLFRSKTSDVTSARLLFGG 240

Query: 331 ALALLLVPLCLPGILSTRRWLARIISTSLSRLIHSRFNLVD---HELHQELIAVGTERNG 390
           ++ LL++PLCLPG++  R W    I +S  RL  S F LVD    E+H+ ++       G
Sbjct: 241 SILLLILPLCLPGLVYARNWYLHNIHSSF-RLEGSGFILVDVDELEMHKGMVTREASLEG 300

Query: 391 MK-----VIIPFDSKEKES--------TSRKVMEKENLVMLEEEHSAKMLLRRLDFWLYY 450
            +     V+   ++ +++S           KV+ +  L ML EEH    LL R DFWLYY
Sbjct: 301 YQLLNDDVVRAVNTPDQKSFIEDDDGCCCTKVITRNQLGMLGEEHPLSFLLCRSDFWLYY 360

Query: 451 VAYFCGGTIGLVYSNNLGQIAQSLGYSSSTSSLVTLYSSCSFFGRLISAAPDFMREKVRF 510
           +AYFCGGTIGLVYSNNLGQIAQSLG SS T++LVTLYSS SFFGRL+SA PD++R KV F
Sbjct: 361 IAYFCGGTIGLVYSNNLGQIAQSLGQSSETTTLVTLYSSFSFFGRLLSATPDYIRAKVYF 420

Query: 511 ARTGWLAIALVPTPIAFILLAASGSKIALQAGTGLIGLSSGFIFSASVSITSELFGPNSS 570
           ARTGWLA+AL+PT IA  LLA+SGS  ALQAGT LIGLSSGFIF+A+VSITSELFGPNS 
Sbjct: 421 ARTGWLAVALLPTTIALFLLASSGSLAALQAGTALIGLSSGFIFAAAVSITSELFGPNSV 480

Query: 571 GVNHNILITNIPLGSFLYGVLAAVAYDSNAESSHQMAALGDAVVCIGQNCYLQTFVWWAC 630
           GVNHNILITNIP+GS +YG LAA+ Y+S++ +  +     ++V+C+G++CYLQTF+WW C
Sbjct: 481 GVNHNILITNIPIGSLVYGFLAALVYESHSVAGSKT----ESVICMGRDCYLQTFMWWGC 540

Query: 631 ISIFGLASSFLLFRRTRPAYDRHYESNLS 644
           +S+ GLASS +LF RTR AY R  +  ++
Sbjct: 541 LSVIGLASSVVLFLRTRRAYQRFEQDRIT 564

BLAST of Cla97C06G109880 vs. TAIR 10
Match: AT1G31470.1 (Major facilitator superfamily protein )

HSP 1 Score: 564.3 bits (1453), Expect = 1.3e-160
Identity = 310/557 (55.66%), Postives = 402/557 (72.17%), Query Frame = 0

Query: 89  KRMLNQPGQWVVLVAAIWIQAFAGTNFDFPSYSSDLKAALGMSQVELNYLAVASDLGKAF 148
           + M  +  +W VLVAAIWIQA  GTNFDF +YSS LK+ LG+SQV LNYLAVASDLGKAF
Sbjct: 35  RTMFGRWRKWTVLVAAIWIQASTGTNFDFSAYSSHLKSVLGISQVRLNYLAVASDLGKAF 94

Query: 149 GWCSGVALLYFPLWVVMFMAASMGFLGYGLQWLLLQRIVYLPYSMVYLLCLMAGCSICWF 208
           GW SG+AL YFPL VV+F AA+MGF+GYG+QWL++  I+ LPYS+V+L CL+AG SICWF
Sbjct: 95  GWSSGIALGYFPLSVVLFAAAAMGFVGYGVQWLVITNIITLPYSLVFLCCLLAGLSICWF 154

Query: 209 NTVCYVSCIQNFPANRALALSLIVSFNGVSAALYTLIANAVDPNDAGLYLFLNALVPLII 268
           NT C++ CI++FP NRALALSL VSFNG+SAALY+L  NA++P+ + LYL LN+LVPL++
Sbjct: 155 NTACFILCIRHFPNNRALALSLTVSFNGISAALYSLAFNAINPSSSNLYLLLNSLVPLVV 214

Query: 269 SIVALLPILHQPSVQPS-SADAIRHDSLIFICLYITAVITGVYLITFNSKPSYKYRSQIL 328
           S  AL P+L +PS+  +   D+ RHDS +F  L + AVIT  +L+  +S  S    +++ 
Sbjct: 215 SFAALYPVLTKPSLDTTPDYDSRRHDSHVFTILNVLAVITSFHLLLSSSSTS---SARLN 274

Query: 329 LAGALALLLVPLCLPGILSTRRWLARIISTSLSRLIHSRFNLVDHELHQELIAVGTERNG 388
             GA+ LL+ PLC P ++  R +   +I+  L+        L   EL  +  +V ++   
Sbjct: 275 FIGAVVLLVFPLCAPLLVYARDYFLPVINARLNHESSGYVMLNIDELKNQKTSVSSK--- 334

Query: 389 MKVIIPFDSKEKESTSRKVMEKENLVMLEEEHSAKMLLRRLDFWLYYVAYFCGGTIGLVY 448
                     E   T++    + N V L +EHS ++L+ RL+FWLYY+AYFCGGTIGLVY
Sbjct: 335 -------TGYEHMGTAK----EGNTVRLGDEHSFRLLISRLEFWLYYIAYFCGGTIGLVY 394

Query: 449 SNNLGQIAQSLGYSSSTSSLVTLYSSCSFFGRLISAAPDFMREKVRFARTGWLAIALVPT 508
           SNNLGQIAQSLG +S+T  LVT+YSS SFFGRL+SAAPDFM ++ R  RTGW AIAL+PT
Sbjct: 395 SNNLGQIAQSLGQNSTT--LVTIYSSFSFFGRLLSAAPDFMHKRFRLTRTGWFAIALLPT 454

Query: 509 PIAFILLAASGS-KIALQAGTGLIGLSSGFIFSASVSITSELFGPNSSGVNHNILITNIP 568
           PIAF LLA S S + ALQ  T LIGLSSGFIF+A+VSITS+LFGPNS GVNHNILITNIP
Sbjct: 455 PIAFFLLAVSSSQQTALQTATALIGLSSGFIFAAAVSITSDLFGPNSVGVNHNILITNIP 514

Query: 569 LGSFLYGVLAAVAYDSNAESSHQMAALGDAVVCIGQNCYLQTFVWWACISIFGLASSFLL 628
           +GS LYG +AA  Y++NA S      + D++VCIG++CY +TFV+W C+SI G+ SS  L
Sbjct: 515 IGSLLYGYIAASIYEANA-SPDITPIVSDSIVCIGRDCYFKTFVFWGCLSILGVVSSLSL 571

Query: 629 FRRTRPAYDRHYESNLS 644
           + RT+P Y R  +  +S
Sbjct: 575 YIRTKPVYHRLEQDKVS 571

BLAST of Cla97C06G109880 vs. TAIR 10
Match: AT3G01630.1 (Major facilitator superfamily protein )

HSP 1 Score: 494.6 bits (1272), Expect = 1.2e-139
Identity = 283/570 (49.65%), Postives = 372/570 (65.26%), Query Frame = 0

Query: 75  RKAPQIPLLPIKKQKRMLNQPGQWVVLVAAIWIQAFAGTNFDFPSYSSDLKAALGMSQVE 134
           RK P  P  P  +   ML    +W VLVAAIWIQAF GTNFDF +YSSD+K+++G+SQ  
Sbjct: 2   RKQPPSPPKP-PRVNSMLQWWRRWTVLVAAIWIQAFTGTNFDFSAYSSDMKSSMGVSQSR 61

Query: 135 LNYLAVASDLGKAFGWCSGVALLYFPLWVVMFMAASMGFLGYGLQWLLLQRIVYLPYSMV 194
           LNY+AVASDLGKA GW SG A+ YFP+  V+F AA+MG +GYG+QWL +  ++ LPYS+V
Sbjct: 62  LNYMAVASDLGKALGWSSGFAIAYFPVSGVLFAAAAMGLVGYGVQWLSIADVIDLPYSLV 121

Query: 195 YLLCLMAGCSICWFNTVCYVSCIQNFPANRALALSLIVSFNGVSAALYTLIANAVDPNDA 254
            + C +AG SICWFNTV ++ CI++F AN +LALSL+VSFNG+SAALYTL   A+    +
Sbjct: 122 LVCCSLAGLSICWFNTVSFILCIRHFKANHSLALSLVVSFNGISAALYTLGHEAISGKSS 181

Query: 255 G---LYLFLNALVPLIISIVALLPILHQPSVQPSSADAIRHDSLIFICLYITAVITGVYL 314
               +YL LN+L+PLI+S++AL P+L  P+   +       ++ IF+   + A++T  YL
Sbjct: 182 ASSDIYLLLNSLIPLIVSVLALWPVLTNPNSSETDTTRTHDETRIFVVFNVLALVTCFYL 241

Query: 315 ITFNSKPSYKYRSQILLAGALALLLVPLCLPGILSTRRWLARIISTSLSRLIHSRFNLVD 374
           +  +S        +    GA+ LLL PLC+P +                  IH       
Sbjct: 242 LLPSSGTYLASSPRWHFLGAIFLLLFPLCVPFL----------------DYIHRALESCF 301

Query: 375 HELHQELIAVGTERNGMKVIIPFDSKEKESTSRKVMEKENLVMLEEEHSAKMLLRRLDFW 434
           H       AV  E   +  I        +S      E+ + V L +EHS  ML+R+L+FW
Sbjct: 302 HHHSSGYAAVNIEEPKILKI--------KSQKSNAEEESDQVRLGDEHSLGMLVRKLEFW 361

Query: 435 LYYVAYFCGGTIGLVYSNNLGQIAQSLGYSSSTS-SLVTLYSSCSFFGRLISAAPDFMRE 494
           LYYVAYFCGGTIGLVYSNNLGQIAQSLG SSS + SLVTL+S+ SF GRL+S+APDF R+
Sbjct: 362 LYYVAYFCGGTIGLVYSNNLGQIAQSLGQSSSNAKSLVTLFSAFSFLGRLLSSAPDFTRK 421

Query: 495 KVRF-ARTGWLAIALVPTPIAFILLAAS---GSKIALQAGTGLIGLSSGFIFSASVSITS 554
           K+++  RTGW  I+L+PTP+AF +LA S        L+  T LIGLSSGF+F+A+VSITS
Sbjct: 422 KLKYLTRTGWFTISLLPTPLAFFILAYSPKTNQTALLEVATALIGLSSGFVFAAAVSITS 481

Query: 555 ELFGPNSSGVNHNILITNIPLGSFLYGVLAAVAYDSNAESSHQMAALGDAVVCIGQNCYL 614
           ELFG NS GVN NILITNIP+GS  YG +A   YD+NA S  + + + D+VVC+G+ CY 
Sbjct: 482 ELFGRNSVGVNQNILITNIPIGSLFYGYMAGSVYDTNA-SLGRKSVVADSVVCVGRKCYF 541

Query: 615 QTFVWWACISIFGLASSFLLFRRTRPAYDR 637
            TF++W C+S+ G   S  LF RTRP Y R
Sbjct: 542 VTFLFWGCLSVLGFVCSLFLFIRTRPVYHR 545

BLAST of Cla97C06G109880 vs. TAIR 10
Match: AT2G30300.1 (Major facilitator superfamily protein )

HSP 1 Score: 276.6 bits (706), Expect = 5.2e-74
Identity = 195/547 (35.65%), Postives = 291/547 (53.20%), Query Frame = 0

Query: 98  WVVLVAAIWIQAFAGTNFDFPSYSSDLKAALGMSQVELNYLAVASDLGKAFGWCSGVALL 157
           W+ LVA +W+Q+F GTN  FP+YSS LK  L +SQ +LNYL+ ASD GK  G+ SG+A +
Sbjct: 8   WLSLVAILWLQSFNGTNLSFPAYSSQLKELLKISQFKLNYLSFASDAGKVLGFISGIAAV 67

Query: 158 YFPLWVVMFMAASMGFLGYGLQWLLLQRIVYLPYSMVYLLCLMAGCSICWFNTVCYVSCI 217
           Y PL +V+    S+GF GYGLQ+L + + +                 ICW NT CY+  I
Sbjct: 68  YLPLPLVLLAGGSLGFAGYGLQYLSIIKKI-----------------ICWINTACYIVAI 127

Query: 218 QNFPANRALALSLIVSFNGVSAALYTLIANAV----DPNDAGLYLFLNALVPLIISIVAL 277
            +FP NR +A+ +  S+ G+S  +YT + ++        +A  YL LN+LVPL+  +V  
Sbjct: 128 NSFPVNRQVAVGITASYQGLSGKIYTDMVHSFFHTSQREEASGYLLLNSLVPLVACLVTA 187

Query: 278 LPILHQPSVQPSSADAIRHDSLIFICLYITAVITGVYLITFN--SKPSYKYRSQILLAGA 337
             ++     +  S    +   + FI L++  + TG+Y +  +  S P+      ++L G 
Sbjct: 188 PMLMRHGGDKTMSYS--KDVKVGFIVLFVLTIATGIYAVATSLVSVPAV-----LVLVGI 247

Query: 338 LALLLVPLCLPGILSTRRWLARIISTSLSRLIHSRFNLVDHELHQELIAVGTERNGMKVI 397
              LL PL +P            I      L+ SR      ++H     V          
Sbjct: 248 ALFLLAPLAIP------------IGVGFKELMSSR--KTQQKVHDLEAPVD--------- 307

Query: 398 IPFDSKEKESTSRKVMEKENLVMLEEEHSAKMLLRRLDFWLYYVAYFCGGTIGLVYSNNL 457
             F   E++ T  +   ++ ++ ++EE     L ++LDFW+Y+  Y  G T+GLV++NNL
Sbjct: 308 -KFYFVEEDHTKEEEEFEKAIIGVKEEVEWTQLWKKLDFWIYFGLYLFGPTVGLVFTNNL 367

Query: 458 GQIAQSLGYSSSTSSLVTLYSSCSFFGRLISAAPD--FMREKVRFARTGWLAIALVPTPI 517
           GQIA+S G S++TSSLV L SS  FFGRL+ +  D  F R K   +    +A +LV    
Sbjct: 368 GQIAESRG-STATSSLVALSSSFGFFGRLLPSLLDYFFSRNKYMPSSPVSMAGSLVAMVA 427

Query: 518 AFILLAASGSKIALQAGTGLIGLSSGFIFSASVSITSELFGPNSSGVNHNILITNIPLGS 577
           +F+LL    S IAL  GT +IG+ SG + S SV++T+ELFG    GVNHNI++ +IPLGS
Sbjct: 428 SFLLLLID-SDIALYIGTAMIGIFSGALTSLSVTMTAELFGTKHFGVNHNIVVGSIPLGS 487

Query: 578 FLYGVLAAVAYDSNAESSHQMAALGDAVVCIGQNCYLQTFVWWACISIFGLASSFLLFRR 637
           F +G+LAA  Y   A      A  GD   C G +C+  T V+W  +       + +L+ R
Sbjct: 488 FSFGLLAAKIYRDGA------ALYGDDGKCFGMHCFQTTLVFWGMLCSIAALLAAVLYIR 498