Cla97C05G101870 (gene) Watermelon (97103) v2.5

Overview
NameCla97C05G101870
Typegene
OrganismCitrullus lanatus subsp. vulgaris cv. 97103 (Watermelon (97103) v2.5)
Descriptionlow-temperature-induced cysteine proteinase-like
LocationCla97Chr05: 30362051 .. 30366388 (+)
RNA-Seq ExpressionCla97C05G101870
SyntenyCla97C05G101870
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: exonCDSpolypeptide
Hold the cursor over a type above to highlight its positions in the sequence below.
ATGGGGACCTACCCATCCTCCCCGATCATGGCTCTCCTCTTCTTCCTCTTCATAGCTTTATCAACTGCGTCGCCTTCTTCCATCATCCCCCAGAGGACAGACGAGGAGGTCATGGTGCTCTACGACCAGTGGCGTGCCAAGCACGGGAAGCTGAACAACCTCTTGGGAGCAAAGCCCGACGATCGATTCCACATCTTCAAGGACAATCTCAAGTACATCGATGAAATCAACGCCCAAAATTTGCCCTACCGATTGGGTTTGAACGTCTTCGCAGATCTGACGAACGAGGAGTACCGATCTAGGTACTTGGGAGGCAAATTCGCCTCAGGTTCAAGGAGAAACAGGACGAGCAACCGCTATCAACCTCGCCTTGGAGACGAAGTCCCCGACTCTATCGATTGGAGGGAGAAAGGAGCCGTTGTTCCCATTAAAGACCAGGGAAGTTGTGGTAAGTTCCATTTTTCTCGCCTTCTTCTTATTTACTTACTTAATGCCTTTACTAGTTTTACTCCTAACTTTGTGTCCTTTTCTTTATTCTCTTTTCTCTAACTTTCTTTTCTCTTTTGCATATTTGGCAAATACACGCACAGACACAACACGTTTTTATTTACCTTTTCAGTATTCAGTATAAATTGCATTGGATTTTAGTTATTTAACTTTAATTAGTAATAGTATTGTCACTAATCCAGTGGTGTAATGGTTTTTAAAAGGTCGATCGATGATCAATCATTATAAGAGGATTGATTGTCCATTTCTTAACTTTGATTTCATGACTAAAAATTGCTGTTGCTGCCTTGTTTTTCCTTCTTTTGTATACAATAGCTTCTGTTTTGATACTTTTGAAATATATGTAACCATCTAGAGAAATAAAAGGTCGGGTAATTTCAATAATATCTATTCTCAAATTTTGAGAAAATTCCAAGATTCCTTGATTTTTATGAACGACCTTTAACATGGGCTTTAGATCAAATTTCAATTCATTAATGAATGAAAAGTGTCTTTAGATTCTATGTGAGATGATACCGTATTAGATAACATGAATTCCAATTCGAAATTAATTTATAATGAGAACAATAACTCATGCCTGCAAAATCTCTTGATTTTTTTTAGATCTACAACATGTTGATTTGATTTAAATGAGTTTTGGAATTGAAAATTTGGAAATCTGTAAAGAGATTGATTTAGGAGAATTGGGTTATCATTATCTGTTTGGCGTGGGGTGCTTTTACTGGGACTGGCTTTCAGGGGGAGCGCTTTATGAGTTGCTCTGCCTTATCTTCAACTCGCACTGGCCCCGCCCAATGACTTTTTTGGTCCTCCATCTACCTTTAATCATGAGATATTTTTTTGAGAGATTTAATCATGATGTTTTCTTTGTAACAATAAGTCACGATATATTTGAAATTTGATGGGTACTTTCATTTATTTTCCAATTATTTCTGAACTACTATCTTTATAAAAATTGTTATTATGTCCATTTTTCAAATATTTTTGGCGCATGACGCAATAATATGCGGCCAATAAGTTTTTATTACCATGCTTTAATTTTTTTTTTTTTTTTGAAAGTTACTATGCTTTAATTTTAGACAATTCACAAAGATTAGTTAATTACATATGTGTATTAGTTATTGAGTTTAAATTCTATATTGGTTCTTGACTTTCCATTTTTCCATTTAACTACTTTCAAAGCATCTCATTGGTCAGTGTCCTTTTTTTTCCTTTTTTAAAAATTACTATTTTAGTCACTATTTTTAAACCTCTAACAGGATGTATGCACAAGAAAAATCATTTAATATAAAAGGCGTGACGATATTTCAAGAAATTGATTAATTATAATATTACTTTGAAATTATGATTAAAAAATGAGATAAAAATGGAAGTTGAAAAAATGGAACAACTCCTTTATAATATGGAGCTTAAATAGATATTCTAAAAGTAAAAGGATTACAAGACGCAATAGTCTTAAGGAATAAGATAAAATAGACAATATAGGATTTAAAATTGAGTTTTTTCTATACTTACAATTATTTCTGAATTATTATTTTAAAAATACTTTGATCTTAGATCTATACTCATTTCAATAGCAGCATCTTCTTTCATGTTATATGATTTTGATGTTTGAAAGTTCTTTTGATATTATCTCAACACAAACCAAGTTTTATTATTTATTATATATTATTTTTTATTTTTAATCTTACACTGATTTTGATCCATTAAAACTATCATACTGTGAGATAGTTTTAGATTTTATTACATTGTCAACATGAAAATAACTCAATAATGATTGACATATATTTTTCTCTTGATATCGGAAGTTCATCCCATACCTCATATTTGTTCTTTAAAAAATCCTATTATATTGGAAATTTATTATAAACAAAAAAAAATCCAGAATTTGAAAATATTTATAGTTATTTTCACGTCAAAACAGTAATTTAGCATATTAACATAAAGTTGGAACTTCAAATACTTTAAATCAATTATTAGATCTGTTATTGATTTATTTATCCTTATATATAGAGAGAAGTAAAGCAACTGTTCAAAGGGATATGTATAATAAATTAATAACTTCATTCTAAGGAGATATTTTTATGGTGCCATTGCCCATAAGCCATACGACAAGGTGGGACCTTGGGCTTGTTCGGCTTTCTGGTTGAAAGCTCTTTCGTAGGCTAAATATATGTTTGCGTCACTGGAATATGTTTTGTTTTCCAATTTAGCCCCTAACTATTTATATATTTTTCAATTTAATCTTTTATATCTAATCTATGACTTTCAATTCTAAATTTTATGCCCTTCAAAATTAAGTTTGGCTGATAGAAAAAGTTGAAACTCTTTTGTAAACTTCTAAGAGAGAGCATACACTTTCTTGACTTATTTTCTCTAAAAATTCTATCAACGGACTTGGTATTAAATTATTTAAATGTGTCATAAATCAACTACAACATCGATCAATAAGAGGTATAATTGAAAATTTTTTATGGAGTAGATTAAAATTTTTTCAAAACATACATTTAGCCCATTTATTATTTTAAAATTATACTCTCAAGGATTCTTGGTCATTGGTCACTTAAAGTAGGCTAATTGTTGAAGTTGATATGAAGGGAGTTGCTGGGCGTTCTCAACTATAGCTGCCGTGGAAGGCATAAACCAGATTGTTACGGGCGACTTGATAGCATTATCTGAACAGGAGCTGGTGGATTGTGACACTTCTTATAACGAAGGTTGCAATGGTGGCTTAATGGACTATGCCTTCGAGTTCATCATCAACAATGGAGGCATTGACACTGAAGAAGATTATCCTTACAACGGATTTGATAGCTCCTGTATCCAATATAAGGTCTCTATTGCCACTCTACTATCTTTTTATTATTTTTTTTTTTCGTAGTTCCAAAAGCATATTTTTGATATCTGATTATCTTTATTTCTATTGTGTTTGCCTCCTTCAGAAAAATGCTAGGGTCGTCTCAATTGATGGTTATGAAGACGTCCCTATAAACAATGAGAAAGCACTGCAAAAAGCAGCAGCTAAACAGGTTGTGGCTGTTGCCATTGAAGGAGGCGGTAGAAGTTTCCAGCTATATCAATCGGTATAACCTCCATTTCTATGAAGTTATTTTTTTATTTTTATTTTTTCCTACTTCAAAAAGAAAAGGGTCTAATTTGTTGTATGTGTATGTGTTTAGGGTATATTCACTGGAAAGTGTGGCACAGATTTGGACCATGGTGTTAATGTGGTTGGATATGGGTCTGAAGGGGGCGTTGATTACTGGATTGTGAGAAACTCATGGGGTGCGAGCTGGGGTGAAAGTGGCTACGTCAGGATGCAACGTAATATCGCTTCCCCAAATGGTCTTTGTGGGATTGCAATGGAGCCTTCCTACCCAATTAAAAACGGACCGAACCCACCAAATCCAGGCCCCACTCCTCCAAGTCCGGTGAAACCCCCATCTGTATGCGATGAATATTACACCTGTCCCGCCGCTGAAACATGCTGCTGTATCTTCGAGTTCTCAAACTTGTGCCTTGAATGGGGATGCTGCCCACTTGAAAGCGCCACCTGTTGTGATGACCATTACAGCTGCTGCCCCCATGACTACCCCATCTGTAACGTCCGTGCTGGAACCTGCTTGAAGGTACGTGGCTTCAATTTCCATCTTTTTATGCATCTTATCAAAACGCTAGAGGTAAATGCCTGAAGAAGAAGACTGAACTTATTTTTGTGTTGTGCAGGGTAAGAATGATGTTTTCGGAGTGAAGGCGATGAGGCGCACTGCAGCTGCAGCCAGACCATCGTGGGCTCATGGAGGTGTTACTGTTGGAAAGAGCAGTGCTTAA

mRNA sequence

ATGGGGACCTACCCATCCTCCCCGATCATGGCTCTCCTCTTCTTCCTCTTCATAGCTTTATCAACTGCGTCGCCTTCTTCCATCATCCCCCAGAGGACAGACGAGGAGGTCATGGTGCTCTACGACCAGTGGCGTGCCAAGCACGGGAAGCTGAACAACCTCTTGGGAGCAAAGCCCGACGATCGATTCCACATCTTCAAGGACAATCTCAAGTACATCGATGAAATCAACGCCCAAAATTTGCCCTACCGATTGGGTTTGAACGTCTTCGCAGATCTGACGAACGAGGAGTACCGATCTAGGTACTTGGGAGGCAAATTCGCCTCAGGTTCAAGGAGAAACAGGACGAGCAACCGCTATCAACCTCGCCTTGGAGACGAAGTCCCCGACTCTATCGATTGGAGGGAGAAAGGAGCCGTTGTTCCCATTAAAGACCAGGGAAGTTGTGGGAGTTGCTGGGCGTTCTCAACTATAGCTGCCGTGGAAGGCATAAACCAGATTGTTACGGGCGACTTGATAGCATTATCTGAACAGGAGCTGGTGGATTGTGACACTTCTTATAACGAAGGTTGCAATGGTGGCTTAATGGACTATGCCTTCGAGTTCATCATCAACAATGGAGGCATTGACACTGAAGAAGATTATCCTTACAACGGATTTGATAGCTCCTGTATCCAATATAAGAAAAATGCTAGGGTCGTCTCAATTGATGGTTATGAAGACGTCCCTATAAACAATGAGAAAGCACTGCAAAAAGCAGCAGCTAAACAGGTTGTGGCTGTTGCCATTGAAGGAGGCGGTAGAAGTTTCCAGCTATATCAATCGGGTATATTCACTGGAAAGTGTGGCACAGATTTGGACCATGGTGTTAATGTGGTTGGATATGGGTCTGAAGGGGGCGTTGATTACTGGATTGTGAGAAACTCATGGGGTGCGAGCTGGGGTGAAAGTGGCTACGTCAGGATGCAACGTAATATCGCTTCCCCAAATGGTCTTTGTGGGATTGCAATGGAGCCTTCCTACCCAATTAAAAACGGACCGAACCCACCAAATCCAGGCCCCACTCCTCCAAGTCCGGTGAAACCCCCATCTGTATGCGATGAATATTACACCTGTCCCGCCGCTGAAACATGCTGCTGTATCTTCGAGTTCTCAAACTTGTGCCTTGAATGGGGATGCTGCCCACTTGAAAGCGCCACCTGTTGTGATGACCATTACAGCTGCTGCCCCCATGACTACCCCATCTGTAACGTCCGTGCTGGAACCTGCTTGAAGGGTAAGAATGATGTTTTCGGAGTGAAGGCGATGAGGCGCACTGCAGCTGCAGCCAGACCATCGTGGGCTCATGGAGGTGTTACTGTTGGAAAGAGCAGTGCTTAA

Coding sequence (CDS)

ATGGGGACCTACCCATCCTCCCCGATCATGGCTCTCCTCTTCTTCCTCTTCATAGCTTTATCAACTGCGTCGCCTTCTTCCATCATCCCCCAGAGGACAGACGAGGAGGTCATGGTGCTCTACGACCAGTGGCGTGCCAAGCACGGGAAGCTGAACAACCTCTTGGGAGCAAAGCCCGACGATCGATTCCACATCTTCAAGGACAATCTCAAGTACATCGATGAAATCAACGCCCAAAATTTGCCCTACCGATTGGGTTTGAACGTCTTCGCAGATCTGACGAACGAGGAGTACCGATCTAGGTACTTGGGAGGCAAATTCGCCTCAGGTTCAAGGAGAAACAGGACGAGCAACCGCTATCAACCTCGCCTTGGAGACGAAGTCCCCGACTCTATCGATTGGAGGGAGAAAGGAGCCGTTGTTCCCATTAAAGACCAGGGAAGTTGTGGGAGTTGCTGGGCGTTCTCAACTATAGCTGCCGTGGAAGGCATAAACCAGATTGTTACGGGCGACTTGATAGCATTATCTGAACAGGAGCTGGTGGATTGTGACACTTCTTATAACGAAGGTTGCAATGGTGGCTTAATGGACTATGCCTTCGAGTTCATCATCAACAATGGAGGCATTGACACTGAAGAAGATTATCCTTACAACGGATTTGATAGCTCCTGTATCCAATATAAGAAAAATGCTAGGGTCGTCTCAATTGATGGTTATGAAGACGTCCCTATAAACAATGAGAAAGCACTGCAAAAAGCAGCAGCTAAACAGGTTGTGGCTGTTGCCATTGAAGGAGGCGGTAGAAGTTTCCAGCTATATCAATCGGGTATATTCACTGGAAAGTGTGGCACAGATTTGGACCATGGTGTTAATGTGGTTGGATATGGGTCTGAAGGGGGCGTTGATTACTGGATTGTGAGAAACTCATGGGGTGCGAGCTGGGGTGAAAGTGGCTACGTCAGGATGCAACGTAATATCGCTTCCCCAAATGGTCTTTGTGGGATTGCAATGGAGCCTTCCTACCCAATTAAAAACGGACCGAACCCACCAAATCCAGGCCCCACTCCTCCAAGTCCGGTGAAACCCCCATCTGTATGCGATGAATATTACACCTGTCCCGCCGCTGAAACATGCTGCTGTATCTTCGAGTTCTCAAACTTGTGCCTTGAATGGGGATGCTGCCCACTTGAAAGCGCCACCTGTTGTGATGACCATTACAGCTGCTGCCCCCATGACTACCCCATCTGTAACGTCCGTGCTGGAACCTGCTTGAAGGGTAAGAATGATGTTTTCGGAGTGAAGGCGATGAGGCGCACTGCAGCTGCAGCCAGACCATCGTGGGCTCATGGAGGTGTTACTGTTGGAAAGAGCAGTGCTTAA

Protein sequence

MGTYPSSPIMALLFFLFIALSTASPSSIIPQRTDEEVMVLYDQWRAKHGKLNNLLGAKPDDRFHIFKDNLKYIDEINAQNLPYRLGLNVFADLTNEEYRSRYLGGKFASGSRRNRTSNRYQPRLGDEVPDSIDWREKGAVVPIKDQGSCGSCWAFSTIAAVEGINQIVTGDLIALSEQELVDCDTSYNEGCNGGLMDYAFEFIINNGGIDTEEDYPYNGFDSSCIQYKKNARVVSIDGYEDVPINNEKALQKAAAKQVVAVAIEGGGRSFQLYQSGIFTGKCGTDLDHGVNVVGYGSEGGVDYWIVRNSWGASWGESGYVRMQRNIASPNGLCGIAMEPSYPIKNGPNPPNPGPTPPSPVKPPSVCDEYYTCPAAETCCCIFEFSNLCLEWGCCPLESATCCDDHYSCCPHDYPICNVRAGTCLKGKNDVFGVKAMRRTAAAARPSWAHGGVTVGKSSA
Homology
BLAST of Cla97C05G101870 vs. NCBI nr
Match: XP_038897503.1 (low-temperature-induced cysteine proteinase-like [Benincasa hispida])

HSP 1 Score: 909.1 bits (2348), Expect = 1.6e-260
Identity = 431/459 (93.90%), Postives = 446/459 (97.17%), Query Frame = 0

Query: 1   MGTYPSSPIMALLFFLFIALSTASPSSIIPQRTDEEVMVLYDQWRAKHGKLNNLLGAKPD 60
           MGTY SSPIMALLFFLFIALS+ASPSSIIP+RTDEEVM LYDQWRAKHGKL N LGA+P+
Sbjct: 1   MGTYLSSPIMALLFFLFIALSSASPSSIIPERTDEEVMALYDQWRAKHGKLRNNLGAEPE 60

Query: 61  DRFHIFKDNLKYIDEINAQNLPYRLGLNVFADLTNEEYRSRYLGGKFASGSRRNRTSNRY 120
           +RFHIFKDNLKYIDEINAQNLPYRLGLNVFADLTNEEYRSRYLGGKFA+GSRRNRTS+RY
Sbjct: 61  NRFHIFKDNLKYIDEINAQNLPYRLGLNVFADLTNEEYRSRYLGGKFAAGSRRNRTSSRY 120

Query: 121 QPRLGDEVPDSIDWREKGAVVPIKDQGSCGSCWAFSTIAAVEGINQIVTGDLIALSEQEL 180
            PRLGDE+PDSIDWREKGAV P+KDQGSCGSCWAFSTIAAVEGINQIVTGDLIALSEQEL
Sbjct: 121 LPRLGDEIPDSIDWREKGAVAPVKDQGSCGSCWAFSTIAAVEGINQIVTGDLIALSEQEL 180

Query: 181 VDCDTSYNEGCNGGLMDYAFEFIINNGGIDTEEDYPYNGFDSSCIQYKKNARVVSIDGYE 240
           VDCD SYNEGCNGGLMDYAFEFIINNGGIDTEEDYPYNGFDSSCIQYKKNARVVSIDGYE
Sbjct: 181 VDCDKSYNEGCNGGLMDYAFEFIINNGGIDTEEDYPYNGFDSSCIQYKKNARVVSIDGYE 240

Query: 241 DVPINNEKALQKAAAKQVVAVAIEGGGRSFQLYQSGIFTGKCGTDLDHGVNVVGYGSEGG 300
           DVPINNEKALQKAA+KQVVAVAIEGGGRSFQLYQSGIFTG+CGTDLDHGVNVVGYGSEGG
Sbjct: 241 DVPINNEKALQKAASKQVVAVAIEGGGRSFQLYQSGIFTGRCGTDLDHGVNVVGYGSEGG 300

Query: 301 VDYWIVRNSWGASWGESGYVRMQRNIASPNGLCGIAMEPSYPIKNGPNPPNPGPTPPSPV 360
           VDYWIVRNSWGASWGESGY+RMQRNIAS  GLCGIAMEPSYP K+GPNPPNPGPTPPSPV
Sbjct: 301 VDYWIVRNSWGASWGESGYIRMQRNIASSTGLCGIAMEPSYPTKSGPNPPNPGPTPPSPV 360

Query: 361 KPPSVCDEYYTCPAAETCCCIFEFSNLCLEWGCCPLESATCCDDHYSCCPHDYPICNVRA 420
           KPPSVCDEYYTCPAAETCCC+FEFS +CLEWGCCPLESATCCDDHYSCCPHDYPICNVRA
Sbjct: 361 KPPSVCDEYYTCPAAETCCCVFEFSTMCLEWGCCPLESATCCDDHYSCCPHDYPICNVRA 420

Query: 421 GTCLKGKNDVFGVKAMRRTAAAARPSWAHGGVTVGKSSA 460
           GTCLKGKNDVFGVKAMRRT AAARPSWAHG VTVGKSSA
Sbjct: 421 GTCLKGKNDVFGVKAMRRTTAAARPSWAHGDVTVGKSSA 459

BLAST of Cla97C05G101870 vs. NCBI nr
Match: XP_008452589.2 (PREDICTED: low-temperature-induced cysteine proteinase-like [Cucumis melo])

HSP 1 Score: 892.1 bits (2304), Expect = 2.0e-255
Identity = 422/459 (91.94%), Postives = 441/459 (96.08%), Query Frame = 0

Query: 1   MGTYPSSPIMALLFFLFIALSTASPSSIIPQRTDEEVMVLYDQWRAKHGKLNNLLGAKPD 60
           MGT+ SS  MALLFFLFIALSTASPSSIIPQRTDEEVM LYDQWRA HGKL+N LGA+P+
Sbjct: 1   MGTFQSSQTMALLFFLFIALSTASPSSIIPQRTDEEVMALYDQWRANHGKLHNNLGAEPE 60

Query: 61  DRFHIFKDNLKYIDEINAQNLPYRLGLNVFADLTNEEYRSRYLGGKFASGSRRNRTSNRY 120
           +RFHIFKDNLKYIDEINAQNLPYRLGLNVFADLTNEEYRSRYLGG+FA+GSRRNRTSNRY
Sbjct: 61  NRFHIFKDNLKYIDEINAQNLPYRLGLNVFADLTNEEYRSRYLGGRFAAGSRRNRTSNRY 120

Query: 121 QPRLGDEVPDSIDWREKGAVVPIKDQGSCGSCWAFSTIAAVEGINQIVTGDLIALSEQEL 180
            PRLGDE+PDSIDWR KGAV P+KDQGSCGSCWAFST+A+VEGINQIVTGDLIALSEQEL
Sbjct: 121 LPRLGDELPDSIDWRAKGAVAPVKDQGSCGSCWAFSTVASVEGINQIVTGDLIALSEQEL 180

Query: 181 VDCDTSYNEGCNGGLMDYAFEFIINNGGIDTEEDYPYNGFDSSCIQYKKNARVVSIDGYE 240
           VDCD SYNEGCNGGLMDYAFEFIINNGG+DTEEDYPY GFDSSCIQYKKNARVVSIDGYE
Sbjct: 181 VDCDKSYNEGCNGGLMDYAFEFIINNGGLDTEEDYPYYGFDSSCIQYKKNARVVSIDGYE 240

Query: 241 DVPINNEKALQKAAAKQVVAVAIEGGGRSFQLYQSGIFTGKCGTDLDHGVNVVGYGSEGG 300
           DVP+NNEKALQKAA+KQVV+VAIEGGGRSFQLYQSGIFTG+CGTDLDHGVNVVGYGSEGG
Sbjct: 241 DVPVNNEKALQKAASKQVVSVAIEGGGRSFQLYQSGIFTGRCGTDLDHGVNVVGYGSEGG 300

Query: 301 VDYWIVRNSWGASWGESGYVRMQRNIASPNGLCGIAMEPSYPIKNGPNPPNPGPTPPSPV 360
           VDYWIVRNSWGA+WGESGYVRMQRNI SP GLCGIAMEPSYP K+GPNPPNPGPTPPSPV
Sbjct: 301 VDYWIVRNSWGANWGESGYVRMQRNIDSPTGLCGIAMEPSYPTKSGPNPPNPGPTPPSPV 360

Query: 361 KPPSVCDEYYTCPAAETCCCIFEFSNLCLEWGCCPLESATCCDDHYSCCPHDYPICNVRA 420
           KPPSVCDEYYTCPAAETCCCIFEFSNLCLEWGCCPLESATCCDDHYSCCPHDYPICNVRA
Sbjct: 361 KPPSVCDEYYTCPAAETCCCIFEFSNLCLEWGCCPLESATCCDDHYSCCPHDYPICNVRA 420

Query: 421 GTCLKGKNDVFGVKAMRRTAAAARPSWAHGGVTVGKSSA 460
           GTC K KND+FGVKAMRRTAAAARPSWAH  VTVGKSSA
Sbjct: 421 GTCSKSKNDIFGVKAMRRTAAAARPSWAHRDVTVGKSSA 459

BLAST of Cla97C05G101870 vs. NCBI nr
Match: KAA0064411.1 (low-temperature-induced cysteine proteinase-like [Cucumis melo var. makuwa] >TYK20176.1 low-temperature-induced cysteine proteinase-like [Cucumis melo var. makuwa])

HSP 1 Score: 883.2 bits (2281), Expect = 9.3e-253
Identity = 417/450 (92.67%), Postives = 435/450 (96.67%), Query Frame = 0

Query: 10  MALLFFLFIALSTASPSSIIPQRTDEEVMVLYDQWRAKHGKLNNLLGAKPDDRFHIFKDN 69
           MALLFFLFIALSTASPSSIIPQRTDEEVM LYDQWRA HGKL+N LGA+P++RFHIFKDN
Sbjct: 1   MALLFFLFIALSTASPSSIIPQRTDEEVMALYDQWRANHGKLHNNLGAEPENRFHIFKDN 60

Query: 70  LKYIDEINAQNLPYRLGLNVFADLTNEEYRSRYLGGKFASGSRRNRTSNRYQPRLGDEVP 129
           LKYIDEINAQNLPYRLGLNVFADLTNEEYRSRYLGG+FA+GSRRNRTSNRY PRLGDE+P
Sbjct: 61  LKYIDEINAQNLPYRLGLNVFADLTNEEYRSRYLGGRFAAGSRRNRTSNRYLPRLGDELP 120

Query: 130 DSIDWREKGAVVPIKDQGSCGSCWAFSTIAAVEGINQIVTGDLIALSEQELVDCDTSYNE 189
           DSIDWR KGAV P+KDQGSCGSCWAFST+A+VEGINQIVTGDLIALSEQELVDCD SYNE
Sbjct: 121 DSIDWRAKGAVAPVKDQGSCGSCWAFSTVASVEGINQIVTGDLIALSEQELVDCDKSYNE 180

Query: 190 GCNGGLMDYAFEFIINNGGIDTEEDYPYNGFDSSCIQYKKNARVVSIDGYEDVPINNEKA 249
           GCNGGLMDYAFEFIINNGG+DTEEDYPY GFDSSCIQYKKNARVVSIDGYEDVP+NNEKA
Sbjct: 181 GCNGGLMDYAFEFIINNGGLDTEEDYPYYGFDSSCIQYKKNARVVSIDGYEDVPVNNEKA 240

Query: 250 LQKAAAKQVVAVAIEGGGRSFQLYQSGIFTGKCGTDLDHGVNVVGYGSEGGVDYWIVRNS 309
           LQKAA+KQVV+VAIEGGGRSFQLYQSGIFTG+CGTDLDHGVNVVGYGSEGGVDYWIVRNS
Sbjct: 241 LQKAASKQVVSVAIEGGGRSFQLYQSGIFTGRCGTDLDHGVNVVGYGSEGGVDYWIVRNS 300

Query: 310 WGASWGESGYVRMQRNIASPNGLCGIAMEPSYPIKNGPNPPNPGPTPPSPVKPPSVCDEY 369
           WGA+WGESGYVRMQRNI SP GLCGIAMEPSYP K+GPNPPNPGPTPPSPVKPPSVCDEY
Sbjct: 301 WGANWGESGYVRMQRNIDSPTGLCGIAMEPSYPTKSGPNPPNPGPTPPSPVKPPSVCDEY 360

Query: 370 YTCPAAETCCCIFEFSNLCLEWGCCPLESATCCDDHYSCCPHDYPICNVRAGTCLKGKND 429
           YTCPAAETCCCIFEFSNLCLEWGCCPLESATCCDDHYSCCPHDYPICNVRAGTC K KND
Sbjct: 361 YTCPAAETCCCIFEFSNLCLEWGCCPLESATCCDDHYSCCPHDYPICNVRAGTCSKSKND 420

Query: 430 VFGVKAMRRTAAAARPSWAHGGVTVGKSSA 460
           +FGVKAMRRTAAAARPSWAH  VTVGKSSA
Sbjct: 421 IFGVKAMRRTAAAARPSWAHRDVTVGKSSA 450

BLAST of Cla97C05G101870 vs. NCBI nr
Match: XP_004141271.2 (low-temperature-induced cysteine proteinase [Cucumis sativus] >KGN55227.1 hypothetical protein Csa_012572 [Cucumis sativus])

HSP 1 Score: 882.9 bits (2280), Expect = 1.2e-252
Identity = 415/459 (90.41%), Postives = 438/459 (95.42%), Query Frame = 0

Query: 1   MGTYPSSPIMALLFFLFIALSTASPSSIIPQRTDEEVMVLYDQWRAKHGKLNNLLGAKPD 60
           MGT+ SSPIMALLFFLFIALS ASPSSIIPQRTD+EVM LYDQWRAKHGKL+N LGA+P+
Sbjct: 1   MGTFQSSPIMALLFFLFIALSAASPSSIIPQRTDDEVMALYDQWRAKHGKLHNNLGAEPE 60

Query: 61  DRFHIFKDNLKYIDEINAQNLPYRLGLNVFADLTNEEYRSRYLGGKFASGSRRNRTSNRY 120
           +RFHIFKDNLK+IDEINAQNLPYRLGLNVFADLTNEEYRSRYLGGKFASGSRRNRTSNRY
Sbjct: 61  NRFHIFKDNLKFIDEINAQNLPYRLGLNVFADLTNEEYRSRYLGGKFASGSRRNRTSNRY 120

Query: 121 QPRLGDEVPDSIDWREKGAVVPIKDQGSCGSCWAFSTIAAVEGINQIVTGDLIALSEQEL 180
            PRLGD++PDSIDWR KGAV P+KDQGSCGSCWAFST+A+VE INQIVTGDLIALSEQEL
Sbjct: 121 LPRLGDDLPDSIDWRAKGAVAPVKDQGSCGSCWAFSTVASVEAINQIVTGDLIALSEQEL 180

Query: 181 VDCDTSYNEGCNGGLMDYAFEFIINNGGIDTEEDYPYNGFDSSCIQYKKNARVVSIDGYE 240
           VDCD SYNEGCNGGLMDYAFEFII NGG+DTEEDYPY GFDSSCIQYKKNA+VV+IDGYE
Sbjct: 181 VDCDRSYNEGCNGGLMDYAFEFIIENGGLDTEEDYPYYGFDSSCIQYKKNAKVVAIDGYE 240

Query: 241 DVPINNEKALQKAAAKQVVAVAIEGGGRSFQLYQSGIFTGKCGTDLDHGVNVVGYGSEGG 300
           DVP+NNEKALQKA +KQVV+VAIEGGGRSFQLYQSGIFTG+CGTDLDHGVNVVGYGSEGG
Sbjct: 241 DVPVNNEKALQKAVSKQVVSVAIEGGGRSFQLYQSGIFTGRCGTDLDHGVNVVGYGSEGG 300

Query: 301 VDYWIVRNSWGASWGESGYVRMQRNIASPNGLCGIAMEPSYPIKNGPNPPNPGPTPPSPV 360
           VDYWIVRNSWG SWGESGYV+MQRNIASP GLCGIAMEPSYP K GPNPPNPGPTPPSPV
Sbjct: 301 VDYWIVRNSWGGSWGESGYVKMQRNIASPTGLCGIAMEPSYPTKTGPNPPNPGPTPPSPV 360

Query: 361 KPPSVCDEYYTCPAAETCCCIFEFSNLCLEWGCCPLESATCCDDHYSCCPHDYPICNVRA 420
           KPPSVCDEYYTCPAAETCCCIF+FSNLCLEWGCCPLESATCCDDHYSCCPHDYP+CNVRA
Sbjct: 361 KPPSVCDEYYTCPAAETCCCIFQFSNLCLEWGCCPLESATCCDDHYSCCPHDYPVCNVRA 420

Query: 421 GTCLKGKNDVFGVKAMRRTAAAARPSWAHGGVTVGKSSA 460
           GTC K KND+FGVKAMRRTAAAARPSWA   VTVGKSSA
Sbjct: 421 GTCSKSKNDIFGVKAMRRTAAAARPSWARRDVTVGKSSA 459

BLAST of Cla97C05G101870 vs. NCBI nr
Match: KAG6608453.1 (Cysteine proteinase RD21A, partial [Cucurbita argyrosperma subsp. sororia] >KAG7037789.1 Cysteine proteinase RD21A [Cucurbita argyrosperma subsp. argyrosperma])

HSP 1 Score: 862.8 bits (2228), Expect = 1.3e-246
Identity = 411/461 (89.15%), Postives = 432/461 (93.71%), Query Frame = 0

Query: 1   MGTYPSSPIMALLFFLFIALST--ASPSSIIPQRTDEEVMVLYDQWRAKHGKLNNLLGAK 60
           MGTY SS IM LLFF+ I LS+  A  SSII +RTDEEVM LYDQWRAKHGKL N L  +
Sbjct: 1   MGTYRSSQIMPLLFFVVITLSSALAFASSIISERTDEEVMALYDQWRAKHGKLRNGLRGQ 60

Query: 61  PDDRFHIFKDNLKYIDEINAQNLPYRLGLNVFADLTNEEYRSRYLGGKFASGSRRNRTSN 120
            DDRF IFKDNLKYIDEINAQNLPYRLGLNVFADLTN EYRS+YLG +FA+GSRRN+TS+
Sbjct: 61  RDDRFEIFKDNLKYIDEINAQNLPYRLGLNVFADLTNAEYRSKYLGTRFAAGSRRNKTSS 120

Query: 121 RYQPRLGDEVPDSIDWREKGAVVPIKDQGSCGSCWAFSTIAAVEGINQIVTGDLIALSEQ 180
           RYQPRLGD++PDS+DWREKGAVVP+KDQGSCGSCWAFSTIAAVEGINQ+VTGDL++LSEQ
Sbjct: 121 RYQPRLGDKLPDSVDWREKGAVVPVKDQGSCGSCWAFSTIAAVEGINQVVTGDLVSLSEQ 180

Query: 181 ELVDCDTSYNEGCNGGLMDYAFEFIINNGGIDTEEDYPYNGFDSSCIQYKKNARVVSIDG 240
           ELVDCDTSYNEGCNGGLMDYAFEFIINNGGIDTEEDYPYNGFDSSCIQYKKNARVVSIDG
Sbjct: 181 ELVDCDTSYNEGCNGGLMDYAFEFIINNGGIDTEEDYPYNGFDSSCIQYKKNARVVSIDG 240

Query: 241 YEDVPINNEKALQKAAAKQVVAVAIEGGGRSFQLYQSGIFTGKCGTDLDHGVNVVGYGSE 300
           YEDVP+N+EKALQKAAA QVVAVAIEGGGRSFQLYQSGIFTGKCGT+LDHGVNVVGYGSE
Sbjct: 241 YEDVPVNDEKALQKAAANQVVAVAIEGGGRSFQLYQSGIFTGKCGTNLDHGVNVVGYGSE 300

Query: 301 GGVDYWIVRNSWGASWGESGYVRMQRNIASPNGLCGIAMEPSYPIKNGPNPPNPGPTPPS 360
           GGVDYWIVRNSWG SWGE GY+RMQRNI SP GLCGIAMEPSYP KNGPNPPNPGPTPPS
Sbjct: 301 GGVDYWIVRNSWGGSWGEHGYIRMQRNIDSPTGLCGIAMEPSYPTKNGPNPPNPGPTPPS 360

Query: 361 PVKPPSVCDEYYTCPAAETCCCIFEFSNLCLEWGCCPLESATCCDDHYSCCPHDYPICNV 420
           PVKPPSVCDEYYTCPAAETCCCIFEFSNLCLEWGCCPLESATCCDDHYSCCPHDYPICNV
Sbjct: 361 PVKPPSVCDEYYTCPAAETCCCIFEFSNLCLEWGCCPLESATCCDDHYSCCPHDYPICNV 420

Query: 421 RAGTCLKGKNDVFGVKAMRRTAAAARPSWAHGGVTVGKSSA 460
           RAGTCLKGKNDVFGVKAMRRT AAA+PSWA G V VGKSSA
Sbjct: 421 RAGTCLKGKNDVFGVKAMRRTTAAAKPSWAFGDVAVGKSSA 461

BLAST of Cla97C05G101870 vs. ExPASy Swiss-Prot
Match: P43297 (Cysteine proteinase RD21A OS=Arabidopsis thaliana OX=3702 GN=RD21A PE=1 SV=1)

HSP 1 Score: 627.5 bits (1617), Expect = 1.2e-178
Identity = 298/457 (65.21%), Postives = 354/457 (77.46%), Query Frame = 0

Query: 8   PIMALLFFLFIALSTASPSSIIPQ-----------RTDEEVMVLYDQWRAKHGKL---NN 67
           P MA+LF   +A+S+A   SII             R++ EVM +Y+ W  KHGK    N+
Sbjct: 6   PTMAILFLAMVAVSSAVDMSIISYDEKHGVSTTGGRSEAEVMSIYEAWLVKHGKAQSQNS 65

Query: 68  LLGAKPDDRFHIFKDNLKYIDEINAQNLPYRLGLNVFADLTNEEYRSRYLGGKFASGSRR 127
           L+  + D RF IFKDNL+++DE N +NL YRLGL  FADLTN+EYRS+YLG K      R
Sbjct: 66  LV--EKDRRFEIFKDNLRFVDEHNEKNLSYRLGLTRFADLTNDEYRSKYLGAKMEKKGER 125

Query: 128 NRTSNRYQPRLGDEVPDSIDWREKGAVVPIKDQGSCGSCWAFSTIAAVEGINQIVTGDLI 187
            RTS RY+ R+GDE+P+SIDWR+KGAV  +KDQG CGSCWAFSTI AVEGINQIVTGDLI
Sbjct: 126 -RTSLRYEARVGDELPESIDWRKKGAVAEVKDQGGCGSCWAFSTIGAVEGINQIVTGDLI 185

Query: 188 ALSEQELVDCDTSYNEGCNGGLMDYAFEFIINNGGIDTEEDYPYNGFDSSCIQYKKNARV 247
            LSEQELVDCDTSYNEGCNGGLMDYAFEFII NGGIDT++DYPY G D +C Q +KNA+V
Sbjct: 186 TLSEQELVDCDTSYNEGCNGGLMDYAFEFIIKNGGIDTDKDYPYKGVDGTCDQIRKNAKV 245

Query: 248 VSIDGYEDVPINNEKALQKAAAKQVVAVAIEGGGRSFQLYQSGIFTGKCGTDLDHGVNVV 307
           V+ID YEDVP  +E++L+KA A Q +++AIE GGR+FQLY SGIF G CGT LDHGV  V
Sbjct: 246 VTIDSYEDVPTYSEESLKKAVAHQPISIAIEAGGRAFQLYDSGIFDGSCGTQLDHGVVAV 305

Query: 308 GYGSEGGVDYWIVRNSWGASWGESGYVRMQRNIASPNGLCGIAMEPSYPIKNGPNPPNPG 367
           GYG+E G DYWIVRNSWG SWGESGY+RM RNIAS +G CGIA+EPSYPIKNG NPPNPG
Sbjct: 306 GYGTENGKDYWIVRNSWGKSWGESGYLRMARNIASSSGKCGIAIEPSYPIKNGENPPNPG 365

Query: 368 PTPPSPVKPPSVCDEYYTCPAAETCCCIFEFSNLCLEWGCCPLESATCCDDHYSCCPHDY 427
           P+PPSP+KPP+ CD YYTCP + TCCC+FE+   C  WGCCPLE+ATCCDD+YSCCPH+Y
Sbjct: 366 PSPPSPIKPPTQCDSYYTCPESNTCCCLFEYGKYCFAWGCCPLEAATCCDDNYSCCPHEY 425

Query: 428 PICNVRAGTCLKGKNDVFGVKAMRRTAAAARPSWAHG 451
           P+C++  GTCL  KN  F VKA++R  A   P W+ G
Sbjct: 426 PVCDLDQGTCLLSKNSPFSVKALKRKPAT--PFWSQG 457

BLAST of Cla97C05G101870 vs. ExPASy Swiss-Prot
Match: Q9FMH8 (Probable cysteine protease RD21B OS=Arabidopsis thaliana OX=3702 GN=RD21B PE=1 SV=1)

HSP 1 Score: 605.5 bits (1560), Expect = 4.9e-172
Identity = 292/462 (63.20%), Postives = 348/462 (75.32%), Query Frame = 0

Query: 1   MGTYPSSPIMALLFFLFIALSTASPSSII-----------PQRTDEEVMVLYDQWRAKHG 60
           MG    SP++ LL    I +S A   SII             R+D EV  +Y+ W  +HG
Sbjct: 1   MGFLKLSPMILLL--AMIGVSYAMDMSIISYDENHHITTETSRSDSEVERIYEAWMVEHG 60

Query: 61  KL---NNLLGAKPDDRFHIFKDNLKYIDEINAQNLPYRLGLNVFADLTNEEYRSRYLGGK 120
           K     N LGA+ D RF IFKDNL++IDE N +NL Y+LGL  FADLTNEEYRS YLG K
Sbjct: 61  KKKMNQNGLGAEKDQRFEIFKDNLRFIDEHNTKNLSYKLGLTRFADLTNEEYRSMYLGAK 120

Query: 121 FASGSRRNRTSNRYQPRLGDEVPDSIDWREKGAVVPIKDQGSCGSCWAFSTIAAVEGINQ 180
                R  +TS+RYQ R+GD +PDS+DWR++GAV  +KDQGSCGSCWAFSTI AVEGIN+
Sbjct: 121 --PTKRVLKTSDRYQARVGDALPDSVDWRKEGAVADVKDQGSCGSCWAFSTIGAVEGINK 180

Query: 181 IVTGDLIALSEQELVDCDTSYNEGCNGGLMDYAFEFIINNGGIDTEEDYPYNGFDSSCIQ 240
           IVTGDLI+LSEQELVDCDTSYN+GCNGGLMDYAFEFII NGGIDTE DYPY   D  C Q
Sbjct: 181 IVTGDLISLSEQELVDCDTSYNQGCNGGLMDYAFEFIIKNGGIDTEADYPYKAADGRCDQ 240

Query: 241 YKKNARVVSIDGYEDVPINNEKALQKAAAKQVVAVAIEGGGRSFQLYQSGIFTGKCGTDL 300
            +KNA+VV+ID YEDVP N+E +L+KA A Q ++VAIE GGR+FQLY SG+F G CGT+L
Sbjct: 241 NRKNAKVVTIDSYEDVPENSEASLKKALAHQPISVAIEAGGRAFQLYSSGVFDGLCGTEL 300

Query: 301 DHGVNVVGYGSEGGVDYWIVRNSWGASWGESGYVRMQRNIASPNGLCGIAMEPSYPIKNG 360
           DHGV  VGYG+E G DYWIVRNSWG  WGESGY++M RNI +P G CGIAME SYPIK G
Sbjct: 301 DHGVVAVGYGTENGKDYWIVRNSWGNRWGESGYIKMARNIEAPTGKCGIAMEASYPIKKG 360

Query: 361 PNPPNPGPTPPSPVKPPSVCDEYYTCPAAETCCCIFEFSNLCLEWGCCPLESATCCDDHY 420
            NPPNPGP+PPSP+KPP+ CD+Y++CP + TCCC++++   C  WGCCPLE+ATCCDD+ 
Sbjct: 361 QNPPNPGPSPPSPIKPPTTCDKYFSCPESNTCCCLYKYGKYCFGWGCCPLEAATCCDDNS 420

Query: 421 SCCPHDYPICNVRAGTCLKGKNDVFGVKAMRRTAAAARPSWA 449
           SCCPH+YP+C+V  GTCL  KN  F VKA++RT A   P WA
Sbjct: 421 SCCPHEYPVCDVNRGTCLMSKNSPFSVKALKRTPAI--PFWA 456

BLAST of Cla97C05G101870 vs. ExPASy Swiss-Prot
Match: P25776 (Oryzain alpha chain OS=Oryza sativa subsp. japonica OX=39947 GN=Os04g0650000 PE=1 SV=2)

HSP 1 Score: 592.4 bits (1526), Expect = 4.3e-168
Identity = 291/459 (63.40%), Postives = 343/459 (74.73%), Query Frame = 0

Query: 6   SSPIMALLFFLFIALSTASPSSI-IPQRTDEEVMVLYDQWRAKHGKLNNLLGAKPDDRFH 65
           S  + A    L ++L+ A  S +   +R++EE   LY +W+A+HGK  N +G + + R+ 
Sbjct: 4   SMALAAAALLLLLSLAAADMSIVSYGERSEEEARRLYAEWKAEHGKSYNAVG-EEERRYA 63

Query: 66  IFKDNLKYIDEINAQN----LPYRLGLNVFADLTNEEYRSRYLGGKFASGSRRNRTSNRY 125
            F+DNL+YIDE NA        +RLGLN FADLTNEEYR  YLG +     R  + S+RY
Sbjct: 64  AFRDNLRYIDEHNAAADAGVHSFRLGLNRFADLTNEEYRDTYLGLR-NKPRRERKVSDRY 123

Query: 126 QPRLGDEVPDSIDWREKGAVVPIKDQGSCGSCWAFSTIAAVEGINQIVTGDLIALSEQEL 185
                + +P+S+DWR KGAV  IKDQG CGSCWAFS IAAVEGINQIVTGDLI+LSEQEL
Sbjct: 124 LAADNEALPESVDWRTKGAVAEIKDQGGCGSCWAFSAIAAVEGINQIVTGDLISLSEQEL 183

Query: 186 VDCDTSYNEGCNGGLMDYAFEFIINNGGIDTEEDYPYNGFDSSCIQYKKNARVVSIDGYE 245
           VDCDTSYNEGCNGGLMDYAF+FIINNGGIDTE+DYPY G D  C   +KNA+VV+ID YE
Sbjct: 184 VDCDTSYNEGCNGGLMDYAFDFIINNGGIDTEDDYPYKGKDERCDVNRKNAKVVTIDSYE 243

Query: 246 DVPINNEKALQKAAAKQVVAVAIEGGGRSFQLYQSGIFTGKCGTDLDHGVNVVGYGSEGG 305
           DV  N+E +LQKA A Q V+VAIE GGR+FQLY SGIFTGKCGT LDHGV  VGYG+E G
Sbjct: 244 DVTPNSETSLQKAVANQPVSVAIEAGGRAFQLYSSGIFTGKCGTALDHGVAAVGYGTENG 303

Query: 306 VDYWIVRNSWGASWGESGYVRMQRNIASPNGLCGIAMEPSYPIKNGPNPPNPGPTPPSPV 365
            DYWIVRNSWG SWGESGYVRM+RNI + +G CGIA+EPSYP+K G NPPNPGPTPPSP 
Sbjct: 304 KDYWIVRNSWGKSWGESGYVRMERNIKASSGKCGIAVEPSYPLKKGENPPNPGPTPPSPT 363

Query: 366 KPPSVCDEYYTCPAAETCCCIFEFSNLCLEWGCCPLESATCCDDHYSCCPHDYPICNVRA 425
            PP+VCD YYTCP + TCCCI+E+   C  WGCCPLE ATCCDDHYSCCPH+YPICNV+ 
Sbjct: 364 PPPTVCDNYYTCPDSTTCCCIYEYGKYCYAWGCCPLEGATCCDDHYSCCPHEYPICNVQQ 423

Query: 426 GTCLKGKNDVFGVKAMRRTAAAARPSWAHGGVTVGKSSA 460
           GTCL  K+    VKA++RT A    S+  G    GK S+
Sbjct: 424 GTCLMAKDSPLAVKALKRTLAKPNLSFLFGN---GKKSS 457

BLAST of Cla97C05G101870 vs. ExPASy Swiss-Prot
Match: P25777 (Oryzain beta chain OS=Oryza sativa subsp. japonica OX=39947 GN=Os04g0670200 PE=1 SV=2)

HSP 1 Score: 526.6 bits (1355), Expect = 2.9e-148
Identity = 262/455 (57.58%), Postives = 318/455 (69.89%), Query Frame = 0

Query: 11  ALLFFLFIALSTASPS-SIIPQR------------TDEEVMVLYDQWRAKHGKLN-NLLG 70
           A L  L +  +TA+P  SII               T+ E    YD W A++G  + N LG
Sbjct: 9   AFLLLLIVGAATAAPDMSIISYNAEHGARGLEEGPTEAEARAAYDLWLAENGGGSPNALG 68

Query: 71  AKPDDRFHIFKDNLKYIDEINA---QNLPYRLGLNVFADLTNEEYRSRYLGGKFASGSRR 130
            + + RF +F DNLK++D  NA   +   +RLG+N FADLTNEE+R+ +LG K A  SR 
Sbjct: 69  GEHERRFLVFWDNLKFVDAHNARADERGGFRLGMNRFADLTNEEFRATFLGAKVAERSR- 128

Query: 131 NRTSNRYQPRLGDEVPDSIDWREKGAVVPIKDQGSCGSCWAFSTIAAVEGINQIVTGDLI 190
                RY+    +E+P+S+DWREKGAV P+K+QG CGSCWAFS ++ VE INQ+VTG++I
Sbjct: 129 -AAGERYRHDGVEELPESVDWREKGAVAPVKNQGQCGSCWAFSAVSTVESINQLVTGEMI 188

Query: 191 ALSEQELVDCDTS-YNEGCNGGLMDYAFEFIINNGGIDTEEDYPYNGFDSSCIQYKKNAR 250
            LSEQELV+C T+  N GCNGGLMD AF+FII NGGIDTE+DYPY   D  C   ++NA+
Sbjct: 189 TLSEQELVECSTNGQNSGCNGGLMDDAFDFIIKNGGIDTEDDYPYKAVDGKCDINRENAK 248

Query: 251 VVSIDGYEDVPINNEKALQKAAAKQVVAVAIEGGGRSFQLYQSGIFTGKCGTDLDHGVNV 310
           VVSIDG+EDVP N+EK+LQKA A Q V+VAIE GGR FQLY SG+F+G+CGT LDHGV  
Sbjct: 249 VVSIDGFEDVPQNDEKSLQKAVAHQPVSVAIEAGGREFQLYHSGVFSGRCGTSLDHGVVA 308

Query: 311 VGYGSEGGVDYWIVRNSWGASWGESGYVRMQRNIASPNGLCGIAMEPSYPIKNGPNPPNP 370
           VGYG++ G DYWIVRNSWG  WGESGYVRM+RNI    G CGIAM  SYP K+G NPP P
Sbjct: 309 VGYGTDNGKDYWIVRNSWGPKWGESGYVRMERNINVTTGKCGIAMMASYPTKSGANPPKP 368

Query: 371 GPTPPSPVKPP------SVCDEYYTCPAAETCCCIFEFSNLCLEWGCCPLESATCCDDHY 430
            PTPP+P  PP       VCD+ ++CPA  TCCC F F NLCL WGCCP+E ATCC DH 
Sbjct: 369 SPTPPTPPTPPPPSAPDHVCDDNFSCPAGSTCCCAFGFRNLCLVWGCCPVEGATCCKDHA 428

Query: 431 SCCPHDYPICNVRAGTCLKGKNDVFGVKAMRRTAA 442
           SCCP DYP+CN RAGTC   KN    VKA++RT A
Sbjct: 429 SCCPPDYPVCNTRAGTCSASKNSPLSVKALKRTLA 461

BLAST of Cla97C05G101870 vs. ExPASy Swiss-Prot
Match: P20721 (Low-temperature-induced cysteine proteinase (Fragment) OS=Solanum lycopersicum OX=4081 PE=2 SV=1)

HSP 1 Score: 513.8 bits (1322), Expect = 1.9e-144
Identity = 230/335 (68.66%), Postives = 274/335 (81.79%), Query Frame = 0

Query: 117 SNRYQPRLGDEVPDSIDWREKGAVVPIKDQGSCGSCWAFSTIAAVEGINQIVTGDLIALS 176
           S+RY P++GD +P+SIDWREKG +V +KDQGSCGSCWAFS +AA+E IN IVTG+LI+LS
Sbjct: 7   SDRYLPKVGDSLPESIDWREKGVLVGVKDQGSCGSCWAFSAVAAMESINAIVTGNLISLS 66

Query: 177 EQELVDCDTSYNEGCNGGLMDYAFEFIINNGGIDTEEDYPYNGFDSSCIQYKKNARVVSI 236
           EQELVDCD SYNEGC+GGLMDYAFEF+I NGGIDTEEDYPY   +  C QY+KNA+VV I
Sbjct: 67  EQELVDCDRSYNEGCDGGLMDYAFEFVIKNGGIDTEEDYPYKERNGVCDQYRKNAKVVKI 126

Query: 237 DGYEDVPINNEKALQKAAAKQVVAVAIEGGGRSFQLYQSGIFTGKCGTDLDHGVNVVGYG 296
           D YEDVP+NNEKALQKA A Q V++A+E GGR FQ Y+SGIFTGKCGT +DHGV + GYG
Sbjct: 127 DSYEDVPVNNEKALQKAVAHQPVSIALEAGGRDFQHYKSGIFTGKCGTAVDHGVVIAGYG 186

Query: 297 SEGGVDYWIVRNSWGASWGESGYVRMQRNIASPNGLCGIAMEPSYPIKNGPNPPNPGPTP 356
           +E G+DYWIVRNSWGA+  E+GY+R+QRN++S +GLCG+A+EPSYP+K GPNPP P P+P
Sbjct: 187 TENGMDYWIVRNSWGANCRENGYLRVQRNVSSSSGLCGLAIEPSYPVKTGPNPPKPAPSP 246

Query: 357 PSPVKPPSVCDEYYTCPAAETCCCIFEFSNLCLEWGCCPLESATCCDDHYSCCPHDYPIC 416
           PSPVKPP+ CDEY  C    TCCCI +F   C  WGCCPLE ATCC+DHYSCCPHDYPIC
Sbjct: 247 PSPVKPPTECDEYSQCAVGTTCCCILQFRRSCFSWGCCPLEGATCCEDHYSCCPHDYPIC 306

Query: 417 NVRAGTCLKGKNDVFGVKAMRRTAAAARPSWAHGG 452
           NVR GTC   K +  GVKAM+R  A    ++ +GG
Sbjct: 307 NVRQGTCSMSKGNPLGVKAMKRILAQPIGAFGNGG 341

BLAST of Cla97C05G101870 vs. ExPASy TrEMBL
Match: A0A1S3BV04 (low-temperature-induced cysteine proteinase-like OS=Cucumis melo OX=3656 GN=LOC103493570 PE=3 SV=1)

HSP 1 Score: 892.1 bits (2304), Expect = 9.7e-256
Identity = 422/459 (91.94%), Postives = 441/459 (96.08%), Query Frame = 0

Query: 1   MGTYPSSPIMALLFFLFIALSTASPSSIIPQRTDEEVMVLYDQWRAKHGKLNNLLGAKPD 60
           MGT+ SS  MALLFFLFIALSTASPSSIIPQRTDEEVM LYDQWRA HGKL+N LGA+P+
Sbjct: 1   MGTFQSSQTMALLFFLFIALSTASPSSIIPQRTDEEVMALYDQWRANHGKLHNNLGAEPE 60

Query: 61  DRFHIFKDNLKYIDEINAQNLPYRLGLNVFADLTNEEYRSRYLGGKFASGSRRNRTSNRY 120
           +RFHIFKDNLKYIDEINAQNLPYRLGLNVFADLTNEEYRSRYLGG+FA+GSRRNRTSNRY
Sbjct: 61  NRFHIFKDNLKYIDEINAQNLPYRLGLNVFADLTNEEYRSRYLGGRFAAGSRRNRTSNRY 120

Query: 121 QPRLGDEVPDSIDWREKGAVVPIKDQGSCGSCWAFSTIAAVEGINQIVTGDLIALSEQEL 180
            PRLGDE+PDSIDWR KGAV P+KDQGSCGSCWAFST+A+VEGINQIVTGDLIALSEQEL
Sbjct: 121 LPRLGDELPDSIDWRAKGAVAPVKDQGSCGSCWAFSTVASVEGINQIVTGDLIALSEQEL 180

Query: 181 VDCDTSYNEGCNGGLMDYAFEFIINNGGIDTEEDYPYNGFDSSCIQYKKNARVVSIDGYE 240
           VDCD SYNEGCNGGLMDYAFEFIINNGG+DTEEDYPY GFDSSCIQYKKNARVVSIDGYE
Sbjct: 181 VDCDKSYNEGCNGGLMDYAFEFIINNGGLDTEEDYPYYGFDSSCIQYKKNARVVSIDGYE 240

Query: 241 DVPINNEKALQKAAAKQVVAVAIEGGGRSFQLYQSGIFTGKCGTDLDHGVNVVGYGSEGG 300
           DVP+NNEKALQKAA+KQVV+VAIEGGGRSFQLYQSGIFTG+CGTDLDHGVNVVGYGSEGG
Sbjct: 241 DVPVNNEKALQKAASKQVVSVAIEGGGRSFQLYQSGIFTGRCGTDLDHGVNVVGYGSEGG 300

Query: 301 VDYWIVRNSWGASWGESGYVRMQRNIASPNGLCGIAMEPSYPIKNGPNPPNPGPTPPSPV 360
           VDYWIVRNSWGA+WGESGYVRMQRNI SP GLCGIAMEPSYP K+GPNPPNPGPTPPSPV
Sbjct: 301 VDYWIVRNSWGANWGESGYVRMQRNIDSPTGLCGIAMEPSYPTKSGPNPPNPGPTPPSPV 360

Query: 361 KPPSVCDEYYTCPAAETCCCIFEFSNLCLEWGCCPLESATCCDDHYSCCPHDYPICNVRA 420
           KPPSVCDEYYTCPAAETCCCIFEFSNLCLEWGCCPLESATCCDDHYSCCPHDYPICNVRA
Sbjct: 361 KPPSVCDEYYTCPAAETCCCIFEFSNLCLEWGCCPLESATCCDDHYSCCPHDYPICNVRA 420

Query: 421 GTCLKGKNDVFGVKAMRRTAAAARPSWAHGGVTVGKSSA 460
           GTC K KND+FGVKAMRRTAAAARPSWAH  VTVGKSSA
Sbjct: 421 GTCSKSKNDIFGVKAMRRTAAAARPSWAHRDVTVGKSSA 459

BLAST of Cla97C05G101870 vs. ExPASy TrEMBL
Match: A0A5A7VFD6 (Low-temperature-induced cysteine proteinase-like OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold134G003080 PE=3 SV=1)

HSP 1 Score: 883.2 bits (2281), Expect = 4.5e-253
Identity = 417/450 (92.67%), Postives = 435/450 (96.67%), Query Frame = 0

Query: 10  MALLFFLFIALSTASPSSIIPQRTDEEVMVLYDQWRAKHGKLNNLLGAKPDDRFHIFKDN 69
           MALLFFLFIALSTASPSSIIPQRTDEEVM LYDQWRA HGKL+N LGA+P++RFHIFKDN
Sbjct: 1   MALLFFLFIALSTASPSSIIPQRTDEEVMALYDQWRANHGKLHNNLGAEPENRFHIFKDN 60

Query: 70  LKYIDEINAQNLPYRLGLNVFADLTNEEYRSRYLGGKFASGSRRNRTSNRYQPRLGDEVP 129
           LKYIDEINAQNLPYRLGLNVFADLTNEEYRSRYLGG+FA+GSRRNRTSNRY PRLGDE+P
Sbjct: 61  LKYIDEINAQNLPYRLGLNVFADLTNEEYRSRYLGGRFAAGSRRNRTSNRYLPRLGDELP 120

Query: 130 DSIDWREKGAVVPIKDQGSCGSCWAFSTIAAVEGINQIVTGDLIALSEQELVDCDTSYNE 189
           DSIDWR KGAV P+KDQGSCGSCWAFST+A+VEGINQIVTGDLIALSEQELVDCD SYNE
Sbjct: 121 DSIDWRAKGAVAPVKDQGSCGSCWAFSTVASVEGINQIVTGDLIALSEQELVDCDKSYNE 180

Query: 190 GCNGGLMDYAFEFIINNGGIDTEEDYPYNGFDSSCIQYKKNARVVSIDGYEDVPINNEKA 249
           GCNGGLMDYAFEFIINNGG+DTEEDYPY GFDSSCIQYKKNARVVSIDGYEDVP+NNEKA
Sbjct: 181 GCNGGLMDYAFEFIINNGGLDTEEDYPYYGFDSSCIQYKKNARVVSIDGYEDVPVNNEKA 240

Query: 250 LQKAAAKQVVAVAIEGGGRSFQLYQSGIFTGKCGTDLDHGVNVVGYGSEGGVDYWIVRNS 309
           LQKAA+KQVV+VAIEGGGRSFQLYQSGIFTG+CGTDLDHGVNVVGYGSEGGVDYWIVRNS
Sbjct: 241 LQKAASKQVVSVAIEGGGRSFQLYQSGIFTGRCGTDLDHGVNVVGYGSEGGVDYWIVRNS 300

Query: 310 WGASWGESGYVRMQRNIASPNGLCGIAMEPSYPIKNGPNPPNPGPTPPSPVKPPSVCDEY 369
           WGA+WGESGYVRMQRNI SP GLCGIAMEPSYP K+GPNPPNPGPTPPSPVKPPSVCDEY
Sbjct: 301 WGANWGESGYVRMQRNIDSPTGLCGIAMEPSYPTKSGPNPPNPGPTPPSPVKPPSVCDEY 360

Query: 370 YTCPAAETCCCIFEFSNLCLEWGCCPLESATCCDDHYSCCPHDYPICNVRAGTCLKGKND 429
           YTCPAAETCCCIFEFSNLCLEWGCCPLESATCCDDHYSCCPHDYPICNVRAGTC K KND
Sbjct: 361 YTCPAAETCCCIFEFSNLCLEWGCCPLESATCCDDHYSCCPHDYPICNVRAGTCSKSKND 420

Query: 430 VFGVKAMRRTAAAARPSWAHGGVTVGKSSA 460
           +FGVKAMRRTAAAARPSWAH  VTVGKSSA
Sbjct: 421 IFGVKAMRRTAAAARPSWAHRDVTVGKSSA 450

BLAST of Cla97C05G101870 vs. ExPASy TrEMBL
Match: A0A0A0L3N7 (Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_4G641600 PE=3 SV=1)

HSP 1 Score: 882.9 bits (2280), Expect = 5.9e-253
Identity = 415/459 (90.41%), Postives = 438/459 (95.42%), Query Frame = 0

Query: 1   MGTYPSSPIMALLFFLFIALSTASPSSIIPQRTDEEVMVLYDQWRAKHGKLNNLLGAKPD 60
           MGT+ SSPIMALLFFLFIALS ASPSSIIPQRTD+EVM LYDQWRAKHGKL+N LGA+P+
Sbjct: 1   MGTFQSSPIMALLFFLFIALSAASPSSIIPQRTDDEVMALYDQWRAKHGKLHNNLGAEPE 60

Query: 61  DRFHIFKDNLKYIDEINAQNLPYRLGLNVFADLTNEEYRSRYLGGKFASGSRRNRTSNRY 120
           +RFHIFKDNLK+IDEINAQNLPYRLGLNVFADLTNEEYRSRYLGGKFASGSRRNRTSNRY
Sbjct: 61  NRFHIFKDNLKFIDEINAQNLPYRLGLNVFADLTNEEYRSRYLGGKFASGSRRNRTSNRY 120

Query: 121 QPRLGDEVPDSIDWREKGAVVPIKDQGSCGSCWAFSTIAAVEGINQIVTGDLIALSEQEL 180
            PRLGD++PDSIDWR KGAV P+KDQGSCGSCWAFST+A+VE INQIVTGDLIALSEQEL
Sbjct: 121 LPRLGDDLPDSIDWRAKGAVAPVKDQGSCGSCWAFSTVASVEAINQIVTGDLIALSEQEL 180

Query: 181 VDCDTSYNEGCNGGLMDYAFEFIINNGGIDTEEDYPYNGFDSSCIQYKKNARVVSIDGYE 240
           VDCD SYNEGCNGGLMDYAFEFII NGG+DTEEDYPY GFDSSCIQYKKNA+VV+IDGYE
Sbjct: 181 VDCDRSYNEGCNGGLMDYAFEFIIENGGLDTEEDYPYYGFDSSCIQYKKNAKVVAIDGYE 240

Query: 241 DVPINNEKALQKAAAKQVVAVAIEGGGRSFQLYQSGIFTGKCGTDLDHGVNVVGYGSEGG 300
           DVP+NNEKALQKA +KQVV+VAIEGGGRSFQLYQSGIFTG+CGTDLDHGVNVVGYGSEGG
Sbjct: 241 DVPVNNEKALQKAVSKQVVSVAIEGGGRSFQLYQSGIFTGRCGTDLDHGVNVVGYGSEGG 300

Query: 301 VDYWIVRNSWGASWGESGYVRMQRNIASPNGLCGIAMEPSYPIKNGPNPPNPGPTPPSPV 360
           VDYWIVRNSWG SWGESGYV+MQRNIASP GLCGIAMEPSYP K GPNPPNPGPTPPSPV
Sbjct: 301 VDYWIVRNSWGGSWGESGYVKMQRNIASPTGLCGIAMEPSYPTKTGPNPPNPGPTPPSPV 360

Query: 361 KPPSVCDEYYTCPAAETCCCIFEFSNLCLEWGCCPLESATCCDDHYSCCPHDYPICNVRA 420
           KPPSVCDEYYTCPAAETCCCIF+FSNLCLEWGCCPLESATCCDDHYSCCPHDYP+CNVRA
Sbjct: 361 KPPSVCDEYYTCPAAETCCCIFQFSNLCLEWGCCPLESATCCDDHYSCCPHDYPVCNVRA 420

Query: 421 GTCLKGKNDVFGVKAMRRTAAAARPSWAHGGVTVGKSSA 460
           GTC K KND+FGVKAMRRTAAAARPSWA   VTVGKSSA
Sbjct: 421 GTCSKSKNDIFGVKAMRRTAAAARPSWARRDVTVGKSSA 459

BLAST of Cla97C05G101870 vs. ExPASy TrEMBL
Match: A0A6J1J4D3 (low-temperature-induced cysteine proteinase-like OS=Cucurbita maxima OX=3661 GN=LOC111481130 PE=3 SV=1)

HSP 1 Score: 860.9 bits (2223), Expect = 2.4e-246
Identity = 410/461 (88.94%), Postives = 433/461 (93.93%), Query Frame = 0

Query: 1   MGTYPSSPIMALLFFLFIALST--ASPSSIIPQRTDEEVMVLYDQWRAKHGKLNNLLGAK 60
           MGTY SS IM LLFF+ I LS+  A  SSII +RTDEEVM LYDQWRAKHGKL N L  +
Sbjct: 1   MGTYRSSQIMPLLFFVIITLSSALALASSIISERTDEEVMALYDQWRAKHGKLRNGLRGQ 60

Query: 61  PDDRFHIFKDNLKYIDEINAQNLPYRLGLNVFADLTNEEYRSRYLGGKFASGSRRNRTSN 120
            DDRF IFKDNLKYIDEINAQNLPYRLGLNVFADLTN EYRS+YLG +FA+GSRRN+TS+
Sbjct: 61  RDDRFEIFKDNLKYIDEINAQNLPYRLGLNVFADLTNAEYRSKYLGTRFAAGSRRNKTSS 120

Query: 121 RYQPRLGDEVPDSIDWREKGAVVPIKDQGSCGSCWAFSTIAAVEGINQIVTGDLIALSEQ 180
           RYQPRLGD++P+S+DWREKGAVVP+KDQGSCGSCWAFSTIAAVEGINQ+VTGDL++LSEQ
Sbjct: 121 RYQPRLGDKLPESVDWREKGAVVPVKDQGSCGSCWAFSTIAAVEGINQVVTGDLVSLSEQ 180

Query: 181 ELVDCDTSYNEGCNGGLMDYAFEFIINNGGIDTEEDYPYNGFDSSCIQYKKNARVVSIDG 240
           ELVDCDTSYNEGCNGGLMDYAFEFIINNGGIDTEEDYPYNGFDSSCIQYKKNARVVSIDG
Sbjct: 181 ELVDCDTSYNEGCNGGLMDYAFEFIINNGGIDTEEDYPYNGFDSSCIQYKKNARVVSIDG 240

Query: 241 YEDVPINNEKALQKAAAKQVVAVAIEGGGRSFQLYQSGIFTGKCGTDLDHGVNVVGYGSE 300
           YEDVP+N+EKALQKAAA QVVAVAIEGGGRSFQLYQSGIFTGKCGT+LDHGVNVVGYGSE
Sbjct: 241 YEDVPVNDEKALQKAAANQVVAVAIEGGGRSFQLYQSGIFTGKCGTNLDHGVNVVGYGSE 300

Query: 301 GGVDYWIVRNSWGASWGESGYVRMQRNIASPNGLCGIAMEPSYPIKNGPNPPNPGPTPPS 360
           GGVDYWIVRNSWG SWGE GY+RMQRNIAS  GLCGIAMEPSYPIK GPNPPNPGPTPPS
Sbjct: 301 GGVDYWIVRNSWGGSWGEHGYIRMQRNIASSTGLCGIAMEPSYPIKKGPNPPNPGPTPPS 360

Query: 361 PVKPPSVCDEYYTCPAAETCCCIFEFSNLCLEWGCCPLESATCCDDHYSCCPHDYPICNV 420
           PVKPPSVCDEYYTCPAAETCCCIFEFSNLCLEWGCCPLESATCCDDHYSCCPHDYPICNV
Sbjct: 361 PVKPPSVCDEYYTCPAAETCCCIFEFSNLCLEWGCCPLESATCCDDHYSCCPHDYPICNV 420

Query: 421 RAGTCLKGKNDVFGVKAMRRTAAAARPSWAHGGVTVGKSSA 460
           RAGTCLKGKNDVFGVKAMRRT AAA+PSWA G V+VGKSSA
Sbjct: 421 RAGTCLKGKNDVFGVKAMRRTTAAAKPSWAFGDVSVGKSSA 461

BLAST of Cla97C05G101870 vs. ExPASy TrEMBL
Match: A0A6J1FLR7 (low-temperature-induced cysteine proteinase-like OS=Cucurbita moschata OX=3662 GN=LOC111446375 PE=3 SV=1)

HSP 1 Score: 860.1 bits (2221), Expect = 4.1e-246
Identity = 410/461 (88.94%), Postives = 431/461 (93.49%), Query Frame = 0

Query: 1   MGTYPSSPIMALLFFLFIALST--ASPSSIIPQRTDEEVMVLYDQWRAKHGKLNNLLGAK 60
           MGTY SS IM LLFF+ I LS+  A  SSII +RTDEEVM LYDQWRAKHGKL N L  +
Sbjct: 1   MGTYRSSQIMPLLFFVIITLSSALALASSIISERTDEEVMALYDQWRAKHGKLRNGLRGQ 60

Query: 61  PDDRFHIFKDNLKYIDEINAQNLPYRLGLNVFADLTNEEYRSRYLGGKFASGSRRNRTSN 120
            DDRF IFKDNLKYIDEINAQNLPYRLGLNVFADLTN EYRS+YLG +FA+GSRRN+TS+
Sbjct: 61  RDDRFEIFKDNLKYIDEINAQNLPYRLGLNVFADLTNAEYRSKYLGTRFAAGSRRNKTSS 120

Query: 121 RYQPRLGDEVPDSIDWREKGAVVPIKDQGSCGSCWAFSTIAAVEGINQIVTGDLIALSEQ 180
           RYQPRLGD++PDS+DWREKGAVVP+KDQGSCGSCWAFSTIAAVEGINQ+VTGDL++LSEQ
Sbjct: 121 RYQPRLGDKLPDSVDWREKGAVVPVKDQGSCGSCWAFSTIAAVEGINQVVTGDLVSLSEQ 180

Query: 181 ELVDCDTSYNEGCNGGLMDYAFEFIINNGGIDTEEDYPYNGFDSSCIQYKKNARVVSIDG 240
           ELVDCDTSYNEGCNGGLMDYAFEFIINNGGIDTEEDYPYNGFDSSCIQYKKNARVVSIDG
Sbjct: 181 ELVDCDTSYNEGCNGGLMDYAFEFIINNGGIDTEEDYPYNGFDSSCIQYKKNARVVSIDG 240

Query: 241 YEDVPINNEKALQKAAAKQVVAVAIEGGGRSFQLYQSGIFTGKCGTDLDHGVNVVGYGSE 300
           YEDVP+N+EKALQKAAA QVVAVAIEGGGRSFQLYQSGIFTGKCGT+LDHGVNVVGYGSE
Sbjct: 241 YEDVPVNDEKALQKAAANQVVAVAIEGGGRSFQLYQSGIFTGKCGTNLDHGVNVVGYGSE 300

Query: 301 GGVDYWIVRNSWGASWGESGYVRMQRNIASPNGLCGIAMEPSYPIKNGPNPPNPGPTPPS 360
           GGVDYWIVRNSWG SWGE GY+RMQRNI S  GLCGIAMEPSYP KNGPNPPNPGPTPPS
Sbjct: 301 GGVDYWIVRNSWGGSWGEHGYIRMQRNIDSKTGLCGIAMEPSYPTKNGPNPPNPGPTPPS 360

Query: 361 PVKPPSVCDEYYTCPAAETCCCIFEFSNLCLEWGCCPLESATCCDDHYSCCPHDYPICNV 420
           PVKPPSVCDEYYTCPAAETCCCIFEFSNLCLEWGCCPLESATCCDDHYSCCPHDYPICNV
Sbjct: 361 PVKPPSVCDEYYTCPAAETCCCIFEFSNLCLEWGCCPLESATCCDDHYSCCPHDYPICNV 420

Query: 421 RAGTCLKGKNDVFGVKAMRRTAAAARPSWAHGGVTVGKSSA 460
           RAGTCLKGKNDVFGVKAMRRT AAA+PSWA G V VGKSSA
Sbjct: 421 RAGTCLKGKNDVFGVKAMRRTTAAAKPSWAFGDVAVGKSSA 461

BLAST of Cla97C05G101870 vs. TAIR 10
Match: AT1G47128.1 (Granulin repeat cysteine protease family protein )

HSP 1 Score: 627.5 bits (1617), Expect = 8.6e-180
Identity = 298/457 (65.21%), Postives = 354/457 (77.46%), Query Frame = 0

Query: 8   PIMALLFFLFIALSTASPSSIIPQ-----------RTDEEVMVLYDQWRAKHGKL---NN 67
           P MA+LF   +A+S+A   SII             R++ EVM +Y+ W  KHGK    N+
Sbjct: 6   PTMAILFLAMVAVSSAVDMSIISYDEKHGVSTTGGRSEAEVMSIYEAWLVKHGKAQSQNS 65

Query: 68  LLGAKPDDRFHIFKDNLKYIDEINAQNLPYRLGLNVFADLTNEEYRSRYLGGKFASGSRR 127
           L+  + D RF IFKDNL+++DE N +NL YRLGL  FADLTN+EYRS+YLG K      R
Sbjct: 66  LV--EKDRRFEIFKDNLRFVDEHNEKNLSYRLGLTRFADLTNDEYRSKYLGAKMEKKGER 125

Query: 128 NRTSNRYQPRLGDEVPDSIDWREKGAVVPIKDQGSCGSCWAFSTIAAVEGINQIVTGDLI 187
            RTS RY+ R+GDE+P+SIDWR+KGAV  +KDQG CGSCWAFSTI AVEGINQIVTGDLI
Sbjct: 126 -RTSLRYEARVGDELPESIDWRKKGAVAEVKDQGGCGSCWAFSTIGAVEGINQIVTGDLI 185

Query: 188 ALSEQELVDCDTSYNEGCNGGLMDYAFEFIINNGGIDTEEDYPYNGFDSSCIQYKKNARV 247
            LSEQELVDCDTSYNEGCNGGLMDYAFEFII NGGIDT++DYPY G D +C Q +KNA+V
Sbjct: 186 TLSEQELVDCDTSYNEGCNGGLMDYAFEFIIKNGGIDTDKDYPYKGVDGTCDQIRKNAKV 245

Query: 248 VSIDGYEDVPINNEKALQKAAAKQVVAVAIEGGGRSFQLYQSGIFTGKCGTDLDHGVNVV 307
           V+ID YEDVP  +E++L+KA A Q +++AIE GGR+FQLY SGIF G CGT LDHGV  V
Sbjct: 246 VTIDSYEDVPTYSEESLKKAVAHQPISIAIEAGGRAFQLYDSGIFDGSCGTQLDHGVVAV 305

Query: 308 GYGSEGGVDYWIVRNSWGASWGESGYVRMQRNIASPNGLCGIAMEPSYPIKNGPNPPNPG 367
           GYG+E G DYWIVRNSWG SWGESGY+RM RNIAS +G CGIA+EPSYPIKNG NPPNPG
Sbjct: 306 GYGTENGKDYWIVRNSWGKSWGESGYLRMARNIASSSGKCGIAIEPSYPIKNGENPPNPG 365

Query: 368 PTPPSPVKPPSVCDEYYTCPAAETCCCIFEFSNLCLEWGCCPLESATCCDDHYSCCPHDY 427
           P+PPSP+KPP+ CD YYTCP + TCCC+FE+   C  WGCCPLE+ATCCDD+YSCCPH+Y
Sbjct: 366 PSPPSPIKPPTQCDSYYTCPESNTCCCLFEYGKYCFAWGCCPLEAATCCDDNYSCCPHEY 425

Query: 428 PICNVRAGTCLKGKNDVFGVKAMRRTAAAARPSWAHG 451
           P+C++  GTCL  KN  F VKA++R  A   P W+ G
Sbjct: 426 PVCDLDQGTCLLSKNSPFSVKALKRKPAT--PFWSQG 457

BLAST of Cla97C05G101870 vs. TAIR 10
Match: AT5G43060.1 (Granulin repeat cysteine protease family protein )

HSP 1 Score: 605.5 bits (1560), Expect = 3.5e-173
Identity = 292/462 (63.20%), Postives = 348/462 (75.32%), Query Frame = 0

Query: 1   MGTYPSSPIMALLFFLFIALSTASPSSII-----------PQRTDEEVMVLYDQWRAKHG 60
           MG    SP++ LL    I +S A   SII             R+D EV  +Y+ W  +HG
Sbjct: 1   MGFLKLSPMILLL--AMIGVSYAMDMSIISYDENHHITTETSRSDSEVERIYEAWMVEHG 60

Query: 61  KL---NNLLGAKPDDRFHIFKDNLKYIDEINAQNLPYRLGLNVFADLTNEEYRSRYLGGK 120
           K     N LGA+ D RF IFKDNL++IDE N +NL Y+LGL  FADLTNEEYRS YLG K
Sbjct: 61  KKKMNQNGLGAEKDQRFEIFKDNLRFIDEHNTKNLSYKLGLTRFADLTNEEYRSMYLGAK 120

Query: 121 FASGSRRNRTSNRYQPRLGDEVPDSIDWREKGAVVPIKDQGSCGSCWAFSTIAAVEGINQ 180
                R  +TS+RYQ R+GD +PDS+DWR++GAV  +KDQGSCGSCWAFSTI AVEGIN+
Sbjct: 121 --PTKRVLKTSDRYQARVGDALPDSVDWRKEGAVADVKDQGSCGSCWAFSTIGAVEGINK 180

Query: 181 IVTGDLIALSEQELVDCDTSYNEGCNGGLMDYAFEFIINNGGIDTEEDYPYNGFDSSCIQ 240
           IVTGDLI+LSEQELVDCDTSYN+GCNGGLMDYAFEFII NGGIDTE DYPY   D  C Q
Sbjct: 181 IVTGDLISLSEQELVDCDTSYNQGCNGGLMDYAFEFIIKNGGIDTEADYPYKAADGRCDQ 240

Query: 241 YKKNARVVSIDGYEDVPINNEKALQKAAAKQVVAVAIEGGGRSFQLYQSGIFTGKCGTDL 300
            +KNA+VV+ID YEDVP N+E +L+KA A Q ++VAIE GGR+FQLY SG+F G CGT+L
Sbjct: 241 NRKNAKVVTIDSYEDVPENSEASLKKALAHQPISVAIEAGGRAFQLYSSGVFDGLCGTEL 300

Query: 301 DHGVNVVGYGSEGGVDYWIVRNSWGASWGESGYVRMQRNIASPNGLCGIAMEPSYPIKNG 360
           DHGV  VGYG+E G DYWIVRNSWG  WGESGY++M RNI +P G CGIAME SYPIK G
Sbjct: 301 DHGVVAVGYGTENGKDYWIVRNSWGNRWGESGYIKMARNIEAPTGKCGIAMEASYPIKKG 360

Query: 361 PNPPNPGPTPPSPVKPPSVCDEYYTCPAAETCCCIFEFSNLCLEWGCCPLESATCCDDHY 420
            NPPNPGP+PPSP+KPP+ CD+Y++CP + TCCC++++   C  WGCCPLE+ATCCDD+ 
Sbjct: 361 QNPPNPGPSPPSPIKPPTTCDKYFSCPESNTCCCLYKYGKYCFGWGCCPLEAATCCDDNS 420

Query: 421 SCCPHDYPICNVRAGTCLKGKNDVFGVKAMRRTAAAARPSWA 449
           SCCPH+YP+C+V  GTCL  KN  F VKA++RT A   P WA
Sbjct: 421 SCCPHEYPVCDVNRGTCLMSKNSPFSVKALKRTPAI--PFWA 456

BLAST of Cla97C05G101870 vs. TAIR 10
Match: AT3G19390.1 (Granulin repeat cysteine protease family protein )

HSP 1 Score: 511.9 bits (1317), Expect = 5.3e-145
Identity = 259/448 (57.81%), Postives = 318/448 (70.98%), Query Frame = 0

Query: 1   MGTYPSSPIMALLFF--LFIALSTASPSSIIPQRTDEEVMVLYDQWRAKHGKLNNLLGAK 60
           M T   S  +ALL F  L I+LS  S ++    R + E   +Y++W  ++ K  N LG K
Sbjct: 1   MATSIKSITLALLIFSVLLISLSLGSVTATETTRNEAEARRMYERWLVENRKNYNGLGEK 60

Query: 61  PDDRFHIFKDNLKYIDEINA-QNLPYRLGLNVFADLTNEEYRSRYLGGKFASGSRRNRTS 120
            + RF IFKDNLK+++E ++  N  Y +GL  FADLTN+E+R+ YL  K    +R     
Sbjct: 61  -ERRFEIFKDNLKFVEEHSSIPNRTYEVGLTRFADLTNDEFRAIYLRSKM-ERTRVPVKG 120

Query: 121 NRYQPRLGDEVPDSIDWREKGAVVPIKDQGSCGSCWAFSTIAAVEGINQIVTGDLIALSE 180
            +Y  ++GD +PD+IDWR KGAV P+KDQGSCGSCWAFS I AVEGINQI TG+LI+LSE
Sbjct: 121 EKYLYKVGDSLPDAIDWRAKGAVNPVKDQGSCGSCWAFSAIGAVEGINQIKTGELISLSE 180

Query: 181 QELVDCDTSYNEGCNGGLMDYAFEFIINNGGIDTEEDYPYNGFD-SSCIQYKKNARVVSI 240
           QELVDCDTSYN+GC GGLMDYAF+FII NGGIDTEEDYPY   D + C   KKN RVV+I
Sbjct: 181 QELVDCDTSYNDGCGGGLMDYAFKFIIENGGIDTEEDYPYIATDVNVCNSDKKNTRVVTI 240

Query: 241 DGYEDVPINNEKALQKAAAKQVVAVAIEGGGRSFQLYQSGIFTGKCGTDLDHGVNVVGYG 300
           DGYEDVP N+EK+L+KA A Q ++VAIE GGR+FQLY SG+FTG CGT LDHGV  VGYG
Sbjct: 241 DGYEDVPQNDEKSLKKALANQPISVAIEAGGRAFQLYTSGVFTGTCGTSLDHGVVAVGYG 300

Query: 301 SEGGVDYWIVRNSWGASWGESGYVRMQRNIASPNGLCGIAMEPSYPIK-NGPNPPNPGPT 360
           SEGG DYWIVRNSWG++WGESGY +++RNI   +G CG+AM  SYP K +G NPP P   
Sbjct: 301 SEGGQDYWIVRNSWGSNWGESGYFKLERNIKESSGKCGVAMMASYPTKSSGSNPPKP--- 360

Query: 361 PPSPVKPPSVCDEYYTCPAAETCCCIFEFSNLCLEWGCCPLESATCCDDHYSCCPHDYPI 420
              P   P VCD+  TCPA  TCCC++E++  C  WGCCP ESATCCDD  SCCP  YP+
Sbjct: 361 ---PAPSPVVCDKSNTCPAKSTCCCLYEYNGKCYSWGCCPYESATCCDDGSSCCPQSYPV 420

Query: 421 CNVRAGTCLKGKNDVFGVKAMRRTAAAA 444
           C+++A TC    N    +KA+ R  A A
Sbjct: 421 CDLKANTCRMKGNSPLSIKALTRGPAIA 440

BLAST of Cla97C05G101870 vs. TAIR 10
Match: AT1G09850.1 (xylem bark cysteine peptidase 3 )

HSP 1 Score: 430.6 bits (1106), Expect = 1.5e-120
Identity = 221/434 (50.92%), Postives = 282/434 (64.98%), Query Frame = 0

Query: 6   SSPIMALLFFLFIALSTASPSSIIPQRTDEEVMVLYDQWRAKHGKLNNLLGAKPD--DRF 65
           SS  ++L FF  + +S++S S        +++  L+D W  KHGK     G++ +   R 
Sbjct: 5   SSSFISLTFFFLLLVSSSSSS--------DDISELFDDWCQKHGK---TYGSEEERQQRI 64

Query: 66  HIFKDNLKYIDEIN-AQNLPYRLGLNVFADLTNEEYRSRYLGGKFASGSRRNRTSNRYQP 125
            IFKDN  ++ + N   N  Y L LN FADLT+ E+++  LG   ++ S    +  +   
Sbjct: 65  QIFKDNHDFVTQHNLITNATYSLSLNAFADLTHHEFKASRLGLSVSAPSVIMASKGQ--- 124

Query: 126 RLGD--EVPDSIDWREKGAVVPIKDQGSCGSCWAFSTIAAVEGINQIVTGDLIALSEQEL 185
            LG   +VPDS+DWR+KGAV  +KDQGSCG+CW+FS   A+EGINQIVTGDLI+LSEQEL
Sbjct: 125 SLGGSVKVPDSVDWRKKGAVTNVKDQGSCGACWSFSATGAMEGINQIVTGDLISLSEQEL 184

Query: 186 VDCDTSYNEGCNGGLMDYAFEFIINNGGIDTEEDYPYNGFDSSCIQYKKNARVVSIDGYE 245
           +DCD SYN GCNGGLMDYAFEF+I N GIDTE+DYPY   D +C + K   +VV+ID Y 
Sbjct: 185 IDCDKSYNAGCNGGLMDYAFEFVIKNHGIDTEKDYPYQERDGTCKKDKLKQKVVTIDSYA 244

Query: 246 DVPINNEKALQKAAAKQVVAVAIEGGGRSFQLYQSGIFTGKCGTDLDHGVNVVGYGSEGG 305
            V  N+EKAL +A A Q V+V I G  R+FQLY SGIF+G C T LDH V +VGYGS+ G
Sbjct: 245 GVKSNDEKALMEAVAAQPVSVGICGSERAFQLYSSGIFSGPCSTSLDHAVLIVGYGSQNG 304

Query: 306 VDYWIVRNSWGASWGESGYVRMQRNIASPNGLCGIAMEPSYPIKNGPNPPNPGPTPPSPV 365
           VDYWIV+NSWG SWG  G++ MQRN  + +G+CGI M  SYPIK  PNP  P P+PP P 
Sbjct: 305 VDYWIVKNSWGKSWGMDGFMHMQRNTENSDGVCGINMLASYPIKTHPNP--PPPSPPGPT 364

Query: 366 KPPSVCDEYYTCPAAETCCCIFEFSNLCLEWGCCPLESATCCDDHYSCCPHDYPICNVRA 425
           K    C+ +  C + ETCCC  E   LC  W CC +ESA CC D   CCPHDYP+C+   
Sbjct: 365 K----CNLFTYCSSGETCCCARELFGLCFSWKCCEIESAVCCKDGRHCCPHDYPVCDTTR 418

Query: 426 GTCLKGKNDVFGVK 435
             CLK   +   +K
Sbjct: 425 SLCLKKTGNFTAIK 418

BLAST of Cla97C05G101870 vs. TAIR 10
Match: AT4G36880.1 (cysteine proteinase1 )

HSP 1 Score: 426.4 bits (1095), Expect = 2.9e-119
Identity = 217/365 (59.45%), Postives = 262/365 (71.78%), Query Frame = 0

Query: 5   PSSPIMALLFFLFIALSTASPSSIIPQ----------RTDEEVMVLYDQWRAKHGKLNNL 64
           PS+ +++LL    +    +   SII            RTDEEV  +Y QW A+HGK NN 
Sbjct: 3   PSTKVLSLLLLYVVVSLASGDESIINDHLQLPSDGKWRTDEEVRSIYLQWSAEHGKTNNN 62

Query: 65  LGA---KPDDRFHIFKDNLKYID--EINAQNLPYRLGLNVFADLTNEEYRSRYLGGKFAS 124
                   D RF+IFKDNL++ID    N +N  Y+LGL  F DLTN+EYR  YLG +   
Sbjct: 63  NNGIINDQDKRFNIFKDNLRFIDLHNENNKNATYKLGLTKFTDLTNDEYRKLYLGARTEP 122

Query: 125 GSRRNRTSN---RYQPRL-GDEVPDSIDWREKGAVVPIKDQGSCGSCWAFSTIAAVEGIN 184
             R  +  N   +Y   + G EVP+++DWR+KGAV PIKDQG+CGSCWAFST AAVEGIN
Sbjct: 123 ARRIAKAKNVNQKYSAAVNGKEVPETVDWRQKGAVNPIKDQGTCGSCWAFSTTAAVEGIN 182

Query: 185 QIVTGDLIALSEQELVDCDTSYNEGCNGGLMDYAFEFIINNGGIDTEEDYPYNGFDSSCI 244
           +IVTG+LI+LSEQELVDCD SYN+GCNGGLMDYAF+FI+ NGG++TE+DYPY GF   C 
Sbjct: 183 KIVTGELISLSEQELVDCDKSYNQGCNGGLMDYAFQFIMKNGGLNTEKDYPYRGFGGKCN 242

Query: 245 QYKKNARVVSIDGYEDVPINNEKALQKAAAKQVVAVAIEGGGRSFQLYQSGIFTGKCGTD 304
            + KN+RVVSIDGYEDVP  +E AL+KA + Q V+VAIE GGR FQ YQSGIFTG CGT+
Sbjct: 243 SFLKNSRVVSIDGYEDVPTKDETALKKAISYQPVSVAIEAGGRIFQHYQSGIFTGSCGTN 302

Query: 305 LDHGVNVVGYGSEGGVDYWIVRNSWGASWGESGYVRMQRNI-ASPNGLCGIAMEPSYPIK 350
           LDH V  VGYGSE GVDYWIVRNSWG  WGE GY+RM+RN+ AS +G CGIA+E SYP+K
Sbjct: 303 LDHAVVAVGYGSENGVDYWIVRNSWGPRWGEEGYIRMERNLAASKSGKCGIAVEASYPVK 362

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
XP_038897503.11.6e-26093.90low-temperature-induced cysteine proteinase-like [Benincasa hispida][more]
XP_008452589.22.0e-25591.94PREDICTED: low-temperature-induced cysteine proteinase-like [Cucumis melo][more]
KAA0064411.19.3e-25392.67low-temperature-induced cysteine proteinase-like [Cucumis melo var. makuwa] >TYK... [more]
XP_004141271.21.2e-25290.41low-temperature-induced cysteine proteinase [Cucumis sativus] >KGN55227.1 hypoth... [more]
KAG6608453.11.3e-24689.15Cysteine proteinase RD21A, partial [Cucurbita argyrosperma subsp. sororia] >KAG7... [more]
Match NameE-valueIdentityDescription
P432971.2e-17865.21Cysteine proteinase RD21A OS=Arabidopsis thaliana OX=3702 GN=RD21A PE=1 SV=1[more]
Q9FMH84.9e-17263.20Probable cysteine protease RD21B OS=Arabidopsis thaliana OX=3702 GN=RD21B PE=1 S... [more]
P257764.3e-16863.40Oryzain alpha chain OS=Oryza sativa subsp. japonica OX=39947 GN=Os04g0650000 PE=... [more]
P257772.9e-14857.58Oryzain beta chain OS=Oryza sativa subsp. japonica OX=39947 GN=Os04g0670200 PE=1... [more]
P207211.9e-14468.66Low-temperature-induced cysteine proteinase (Fragment) OS=Solanum lycopersicum O... [more]
Match NameE-valueIdentityDescription
A0A1S3BV049.7e-25691.94low-temperature-induced cysteine proteinase-like OS=Cucumis melo OX=3656 GN=LOC1... [more]
A0A5A7VFD64.5e-25392.67Low-temperature-induced cysteine proteinase-like OS=Cucumis melo var. makuwa OX=... [more]
A0A0A0L3N75.9e-25390.41Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_4G641600 PE=3 SV=1[more]
A0A6J1J4D32.4e-24688.94low-temperature-induced cysteine proteinase-like OS=Cucurbita maxima OX=3661 GN=... [more]
A0A6J1FLR74.1e-24688.94low-temperature-induced cysteine proteinase-like OS=Cucurbita moschata OX=3662 G... [more]
Match NameE-valueIdentityDescription
AT1G47128.18.6e-18065.21Granulin repeat cysteine protease family protein [more]
AT5G43060.13.5e-17363.20Granulin repeat cysteine protease family protein [more]
AT3G19390.15.3e-14557.81Granulin repeat cysteine protease family protein [more]
AT1G09850.11.5e-12050.92xylem bark cysteine peptidase 3 [more]
AT4G36880.12.9e-11959.45cysteine proteinase1 [more]
InterPro
Analysis Name: InterPro Annotations of Watermelon (97103) v2.5
Date Performed: 2022-01-31
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR000668Peptidase C1A, papain C-terminalPRINTSPR00705PAPAINcoord: 288..298
score: 56.8
coord: 303..309
score: 73.06
coord: 146..161
score: 65.92
IPR000668Peptidase C1A, papain C-terminalSMARTSM00645pept_c1coord: 128..343
e-value: 1.6E-121
score: 419.7
IPR000668Peptidase C1A, papain C-terminalPFAMPF00112Peptidase_C1coord: 128..343
e-value: 5.2E-81
score: 271.9
IPR000118GranulinSMARTSM00277GRAN_2coord: 366..423
e-value: 1.0E-30
score: 118.0
IPR000118GranulinPFAMPF00396Granulincoord: 377..424
e-value: 1.9E-11
score: 44.1
IPR013201Cathepsin propeptide inhibitor domain (I29)SMARTSM00848Inhibitor_I29_2coord: 41..98
e-value: 6.1E-15
score: 65.6
IPR013201Cathepsin propeptide inhibitor domain (I29)PFAMPF08246Inhibitor_I29coord: 58..98
e-value: 8.3E-9
score: 35.8
IPR037277Granulin superfamilyGENE3D2.10.25.160Granulincoord: 363..426
e-value: 4.9E-13
score: 51.2
NoneNo IPR availableGENE3D3.90.70.10Cysteine proteinasescoord: 24..345
e-value: 1.5E-113
score: 381.8
NoneNo IPR availablePANTHERPTHR12411CYSTEINE PROTEASE FAMILY C1-RELATEDcoord: 25..373
NoneNo IPR availablePANTHERPTHR12411:SF749CYSTEINE PROTEASEcoord: 25..373
NoneNo IPR availableSUPERFAMILY57277Granulin repeatcoord: 363..397
IPR025661Cysteine peptidase, asparagine active sitePROSITEPS00640THIOL_PROTEASE_ASNcoord: 303..322
IPR000169Cysteine peptidase, cysteine active sitePROSITEPS00139THIOL_PROTEASE_CYScoord: 146..157
IPR025660Cysteine peptidase, histidine active sitePROSITEPS00639THIOL_PROTEASE_HIScoord: 286..296
IPR039417Papain-like cysteine endopeptidaseCDDcd02248Peptidase_C1Acoord: 129..342
e-value: 9.21735E-100
score: 295.687
IPR038765Papain-like cysteine peptidase superfamilySUPERFAMILY54001Cysteine proteinasescoord: 32..343

Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
Cla97C05G101870.1Cla97C05G101870.1mRNA


GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
biological_process GO:0051603 proteolysis involved in cellular protein catabolic process
biological_process GO:0006508 proteolysis
cellular_component GO:0005615 extracellular space
cellular_component GO:0005764 lysosome
molecular_function GO:0004197 cysteine-type endopeptidase activity
molecular_function GO:0008234 cysteine-type peptidase activity