Cla97C05G100120 (gene) Watermelon (97103) v2.5

Overview
NameCla97C05G100120
Typegene
OrganismCitrullus lanatus subsp. vulgaris cv. 97103 (Watermelon (97103) v2.5)
DescriptionBifunctional nuclease 1
LocationCla97Chr05: 29058624 .. 29062544 (+)
RNA-Seq ExpressionCla97C05G100120
SyntenyCla97C05G100120
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: exonCDSpolypeptidethree_prime_UTR
Hold the cursor over a type above to highlight its positions in the sequence below.
ATGGATCTTCAAGCACTTCCGAGTGCTAGGACTGATGATCAAGACCCACTTTATTTCCCACTGACGATAATGATAGATATCCCTTATAGTTCACGAAAAAACGTTGAATCCGACCCTGTGAATCTTTAATCTACTCTTCTTTCTTTGTTTTCCCTGGAAAATTCGTCGCTGGTACTAATATTTGAATTTGTTCAAACTGATGACTCTTAACACTGTTTTAATTTTCATCGCCCAAATTTGTTTACTTCAAATTTCAATTTCCTTTAAGTGGAGACTTCAAGTTTGCAGCGTTCACTAATTATATGCGTTCGAATCGCCAATTCGTAGAGCAAACTCAGTTAGTGCCGTAATTTTGTGTTTTTTGGGTCTAGATTGGGGATTATGTATATTCGTTTTATCTGATCGGTCTCATTTGGGTGAACTGAAGGACGCGATATGGGGACGTTGCAAGGACCAGTTGTTTGCCCTACTATTCGTGCGAAGCAAGCAGGGTTTTGTTCGTTGCCAATCAATCGTTCTTTAATGCCCTTTAGTTTTCGAAGAGGTGAATTATGGGGATTCACGGGGATCAACGGTTTGAATGCTAAGTTACCAGCTATTTCTCTCAAAGGTTGTGCTAGAAAATGCAAGACATTGCGTTGCAGCTTCAGTTCATCATCTGACGACAATGGAAGTACAGCCGAGAACTTCAACGAGAAGGATGAAGAATATGTTGAATCAAGCGTGGTCGAGGCTGGTATGCTAAGCTGATGGCTTAAATCCTATGATTACATTTTATTTTAATGAATAGAGTGTCTATATTTTGGGAATTTAATTCTAGTGTATGTTAATTTGTAGTTAGATGGTGCGATCAACTAATAGAAATGCCTTTTGGTTTGTTTGTGCTGTATAGTTGAGGTGAAAAGTGGTGCCGATGGTTTCGTGATTAAAATGAGGGATGGTAGGCACTTAAGGTGTGTTCACAACAACCCTCACGGTGGGCATCTGCCTGATTATGCTCCACATCCTGCAATTGTGCTGAAGATGGAAGATGGGACTGGCCTTCTTCTTCCCATAATCGTGTGTATGTATTAGTGAGCGATAGTTTCAAACATGAGCTTAACATTGTTGAACAAATTTGACTTTTTTTTTTCTTTATGGCACATCAACATTTCGTCTTTTGCAGTGGAAATGCCCAGTGTGCTGCTTATGGCTGCTGTGCGCAATGTTCCAATTGTATGATTTCTTTATCTTCTCTTGAATTTGGATATAAATTTTTGGAATTTGAATTTTTAACAGTTATTATTATATGGTTCCAGGCCAGACCAACTATGTATCAAGTGGTAAAAGAGATGATTGACAAGATGGGTTATGAAGTATGTTTTACTTCATTTTTCTGTTTTCCCACCTTGTCTTTTGAGTTTACCCTGAAGTATAGCTATAGTATATGTTTATAAAGCTAAGTGGAATGTGGCCTCTTTCTTATGTTAATATTGAAGATTTTATTTATCACTGCAGGTCAAACTTGTAAGGGTTACCAAGAGAGTGCACGAGGCATATTTTGCTCAGTTATACCTCTCAAAGGCATGTATAAATTTCTCCCTCTCAATCATGTACTCTTCTCTTAGGTAAGTTTAAGAGATTAAAATTGTGTGTTTTTCAGATTGGTTGTGACACTGACTGCCTCAGCTTTGATCTCCGACCTTCAGATGCAATAAATATTGCAGTCCGATGCAAGGTAACGTTAACAGTTTTGTGCATGCTTTTTCTTGGTTGAGAATAGAGACTCTGGTCAAGTGCTTCTTTAAGTGGCCGCAAGAATTGCTGAAATGTTTCAGTTAGTACGTGCCATTGAATTGCCTACAAGTGCATAAATATTTTGTTATTTGGGCTTTTGGGTAATTTGGTCATTTTCTATGGGTTATAGAATGTCTCTTTTCATTTGTATGAGTTGTATTTAGATGTTTCTCAAATATTGGGCATGATTTTCTGCTATGATTGCTGAATCATATCACTTCTTGCAATTGATGCAGGTCCCAATTCAAGTGAACAAGTATCTTGCATACAGTGATGGTATGAGAGTTGTTGAAAGTGGGAAGCTGTCAACCCAGGCCCCTGCTACGGATGGCTTATTATTCACTGAACTGGATAGGTAATAAAATATTTCCGCAGTCTCACTTTCTTTAGTTCAAATTAATTTCTAAGAAACATGGACAAGTGGTAAGGTTATTATACAACACAGACAAGGCAAAGTTTTGTTCCAAAAAAATTAGGACAGGGGACATATATTGTTTGAGGATTTTTTATGGACTTCAGTACTTTTCACATTAGTGAGCGCTAGCATTTTAAACTATGGTACATCCATTTAAGATAGAATGTGGTAGAATGGATACAATATGTAACAGTAGAAGAAGTTGAAGTGGCTAATAACATATTAATTGATCAAATATGTTATATCAGAGGTTTTAAATAGAACTGCTACCAGTGTCATACAGTTGTCTCTCTAAATGATTAATTTACTTATAAAAAAAGAAAACTCAAAATAAAATGCTGCCGCTGTTTTATGCTTAAATGAAGAATATTGATATATTTCATTTAAATCTACCATTTTCTGCTTATATCTGCGTTAAACACTGTTTACTATAATTTAACTGTCAGTGTTAGGGTCCTATCTATGTAGGATCCAAAAACGTTACCAAACTGAAGTACTATGCTTTATAATTTAAGTTCGTACAACCTTTCTAGATGGGCTTAGAAATTGATCTCAAGTTTTTCTGTGCAGAGCACAAATTGCTGTGGACAAACTAATGGCCCCTTATTCTGTTGACAGCAATTTGAGACTCTACTTCATTGGACTAGAGAAAATGGTTTAATTGAATTCTTTTGTTTTCAGGCCTAGTGGTCAGCCTTGTGTTGAAGCCAAAGAGTTCAATCTCGTGAGGAATATGCTGATTGCGGCTGTTGAAGAGCGCTATAGAGATGCTGGTACCTATTCTGTTGCTCAATGATATATATATGGTGTTATGACATGATCATCATACTATGTTCATTTATAAATGTTCTAAATCAATTTTATTTTCTTGATTTAGTACTGAAGAAAAAATTAAATATGAAAAATGGAAAGACCAAAAATAATTAATTGCTTTATATGTGATGACCTTGTGTTGTTACACTTTTCCAAACCACAAACAAAATGCCTTCTTTTTTGGATAGTTCTAGAAAATTGTCGTAGGAATCCCTCTGTTTAGACAAATGCTGTTCTAGGACGAAAACAAGCCCCATTGATATGGGATTTTATTCAGTTTTTGAGTTGAGCAATATACATTTAGTTCTCATGAATGGTCTGTATTTCAGCTCAATGGAGGGACAAGCTCAATCTCCTGAGGGCTAGGAGGAATTTGGCATAATAAGAGGAGGTACAGAAGCTCATTTATTCTCCTGTATTAACTGAGATGATGATGACATATCTTACACTTTCTGTATTAAATATCAATATTTCTTCCCCACTTTTTTCTTTTGGTGGAAACAAGAGAATAGAGACTGAAAAATTATTTGCTGTTTCAGTTACTCCTGGAGATAGCATACTTGTACATAGAACGGAATTGTTCGTGGCCGAGGTTGGTTTGCGTGGGTGAACTATATTGCCTCTTGCCTCTTGCCTCTTGCCTCCTCTACTTGCATTATATATTTAGCAACTCTGTGAATATACATATCTATATTATATATATGGTGTAGATAGAACTTGTTGGTTTCGGGTCGTTGGTGTTCTTAGTGAAATGCATGACATAACCAATTGCATTGTTGCTTCACAATCAGATGAATTTGTGAATGAACTACATTGCTTATTTATAGCAGTGGCACATAATCACCAAAGAGTCCCATTTTCCTTTTACTCGCCGACTTATATTTAGTATAAGATGGTTTATATTATATATTAAAAGA

mRNA sequence

ATGGATCTTCAAGCACTTCCGAGTGCTAGGACTGATGATCAAGACCCACTTTATTTCCCACTGACGATAATGATAGATATCCCTTATAGACGCGATATGGGGACGTTGCAAGGACCAGTTGTTTGCCCTACTATTCGTGCGAAGCAAGCAGGGTTTTGTTCGTTGCCAATCAATCGTTCTTTAATGCCCTTTAGTTTTCGAAGAGGTGAATTATGGGGATTCACGGGGATCAACGGTTTGAATGCTAAGTTACCAGCTATTTCTCTCAAAGGTTGTGCTAGAAAATGCAAGACATTGCGTTGCAGCTTCAGTTCATCATCTGACGACAATGGAAGTACAGCCGAGAACTTCAACGAGAAGGATGAAGAATATGTTGAATCAAGCGTGGTCGAGGCTGTTGAGGTGAAAAGTGGTGCCGATGGTTTCGTGATTAAAATGAGGGATGGTAGGCACTTAAGGTGTGTTCACAACAACCCTCACGGTGGGCATCTGCCTGATTATGCTCCACATCCTGCAATTGTGCTGAAGATGGAAGATGGGACTGGCCTTCTTCTTCCCATAATCGTGTTGGAAATGCCCAGTGTGCTGCTTATGGCTGCTGTGCGCAATGTTCCAATTGCCAGACCAACTATGTATCAAGTGGTAAAAGAGATGATTGACAAGATGGGTTATGAAATTGGTTGTGACACTGACTGCCTCAGCTTTGATCTCCGACCTTCAGATGCAATAAATATTGCAGTCCGATGCAAGGTCCCAATTCAAGTGAACAAGTATCTTGCATACAGTGATGGTATGAGAGTTGTTGAAAGTGGGAAGCTGTCAACCCAGGCCCCTGCTACGGATGGCTTATTATTCACTGAACTGGATAGGCCTAGTGGTCAGCCTTGTGTTGAAGCCAAAGAGTTCAATCTCGTGAGGAATATGCTGATTGCGGCTGTTGAAGAGCGCTATAGAGATGCTGTTACTCCTGGAGATAGCATACTTGTACATAGAACGGAATTGTTCGTGGCCGAGGTTGGTTTGCGTGGGTGAACTATATTGCCTCTTGCCTCTTGCCTCTTGCCTCCTCTACTTGCATTATATATTTAGCAACTCTGTGAATATACATATCTATATTATATATATGGTGTAGATAGAACTTGTTGGTTTCGGGTCGTTGGTGTTCTTAGTGAAATGCATGACATAACCAATTGCATTGTTGCTTCACAATCAGATGAATTTGTGAATGAACTACATTGCTTATTTATAGCAGTGGCACATAATCACCAAAGAGTCCCATTTTCCTTTTACTCGCCGACTTATATTTAGTATAAGATGGTTTATATTATATATTAAAAGA

Coding sequence (CDS)

ATGGATCTTCAAGCACTTCCGAGTGCTAGGACTGATGATCAAGACCCACTTTATTTCCCACTGACGATAATGATAGATATCCCTTATAGACGCGATATGGGGACGTTGCAAGGACCAGTTGTTTGCCCTACTATTCGTGCGAAGCAAGCAGGGTTTTGTTCGTTGCCAATCAATCGTTCTTTAATGCCCTTTAGTTTTCGAAGAGGTGAATTATGGGGATTCACGGGGATCAACGGTTTGAATGCTAAGTTACCAGCTATTTCTCTCAAAGGTTGTGCTAGAAAATGCAAGACATTGCGTTGCAGCTTCAGTTCATCATCTGACGACAATGGAAGTACAGCCGAGAACTTCAACGAGAAGGATGAAGAATATGTTGAATCAAGCGTGGTCGAGGCTGTTGAGGTGAAAAGTGGTGCCGATGGTTTCGTGATTAAAATGAGGGATGGTAGGCACTTAAGGTGTGTTCACAACAACCCTCACGGTGGGCATCTGCCTGATTATGCTCCACATCCTGCAATTGTGCTGAAGATGGAAGATGGGACTGGCCTTCTTCTTCCCATAATCGTGTTGGAAATGCCCAGTGTGCTGCTTATGGCTGCTGTGCGCAATGTTCCAATTGCCAGACCAACTATGTATCAAGTGGTAAAAGAGATGATTGACAAGATGGGTTATGAAATTGGTTGTGACACTGACTGCCTCAGCTTTGATCTCCGACCTTCAGATGCAATAAATATTGCAGTCCGATGCAAGGTCCCAATTCAAGTGAACAAGTATCTTGCATACAGTGATGGTATGAGAGTTGTTGAAAGTGGGAAGCTGTCAACCCAGGCCCCTGCTACGGATGGCTTATTATTCACTGAACTGGATAGGCCTAGTGGTCAGCCTTGTGTTGAAGCCAAAGAGTTCAATCTCGTGAGGAATATGCTGATTGCGGCTGTTGAAGAGCGCTATAGAGATGCTGTTACTCCTGGAGATAGCATACTTGTACATAGAACGGAATTGTTCGTGGCCGAGGTTGGTTTGCGTGGGTGA

Protein sequence

MDLQALPSARTDDQDPLYFPLTIMIDIPYRRDMGTLQGPVVCPTIRAKQAGFCSLPINRSLMPFSFRRGELWGFTGINGLNAKLPAISLKGCARKCKTLRCSFSSSSDDNGSTAENFNEKDEEYVESSVVEAVEVKSGADGFVIKMRDGRHLRCVHNNPHGGHLPDYAPHPAIVLKMEDGTGLLLPIIVLEMPSVLLMAAVRNVPIARPTMYQVVKEMIDKMGYEIGCDTDCLSFDLRPSDAINIAVRCKVPIQVNKYLAYSDGMRVVESGKLSTQAPATDGLLFTELDRPSGQPCVEAKEFNLVRNMLIAAVEERYRDAVTPGDSILVHRTELFVAEVGLRG
Homology
BLAST of Cla97C05G100120 vs. NCBI nr
Match: XP_008466529.1 (PREDICTED: bifunctional nuclease 1 [Cucumis melo] >XP_008466530.1 PREDICTED: bifunctional nuclease 1 [Cucumis melo] >KAA0041551.1 bifunctional nuclease 1 [Cucumis melo var. makuwa] >TYK19703.1 bifunctional nuclease 1 [Cucumis melo var. makuwa])

HSP 1 Score: 565.5 bits (1456), Expect = 3.2e-157
Identity = 285/317 (89.91%), Postives = 289/317 (91.17%), Query Frame = 0

Query: 33  MGTLQGPVVCPTIRAKQAGFCSLPINRSLMPFSFRRGELWGFTGINGLNAKLPAISLKGC 92
           MG+LQGPVVCPTIRAKQAGFCSLPINRSLM FSFRRGELWGFTGINGLNAKLPAISLKGC
Sbjct: 1   MGSLQGPVVCPTIRAKQAGFCSLPINRSLMAFSFRRGELWGFTGINGLNAKLPAISLKGC 60

Query: 93  ARKCKTLRCSFSSSSDDNGSTAENFNEKDEEYVESSVVEAVEVKSGADGFVIKMRDGRHL 152
           ARKCKTLRCSFSSSSDDNGSTAENFNEKDEEYVESSVVEAVEVKSGADGFVIKMRDGRHL
Sbjct: 61  ARKCKTLRCSFSSSSDDNGSTAENFNEKDEEYVESSVVEAVEVKSGADGFVIKMRDGRHL 120

Query: 153 RCVHNNPHGGHLPDYAPHPAIVLKMEDGTGLLLPIIVLEMPSVLLMAAVRNVPIARPTMY 212
           RCVHNNPHGGHLPDYAPHPAIVLKMEDGTGLLLPIIVLEMPSVLLMAAVRNVPIARPTMY
Sbjct: 121 RCVHNNPHGGHLPDYAPHPAIVLKMEDGTGLLLPIIVLEMPSVLLMAAVRNVPIARPTMY 180

Query: 213 QVVKEMIDKMGYE----------------------IGCDTDCLSFDLRPSDAINIAVRCK 272
           QVVKEM+DKMGYE                      IGC+TDCLSFDLRPSDAINIAVRCK
Sbjct: 181 QVVKEMVDKMGYEVKLVRVTKRVHEAYFAQLYLSKIGCETDCLSFDLRPSDAINIAVRCK 240

Query: 273 VPIQVNKYLAYSDGMRVVESGKLSTQAPATDGLLFTELDRPSGQPCVEAKEFNLVRNMLI 328
           VPIQVNKYLAYSDGMRVVESGKLSTQAPATDGLLFTELDRPSGQPCVEAKEFNLVRNMLI
Sbjct: 241 VPIQVNKYLAYSDGMRVVESGKLSTQAPATDGLLFTELDRPSGQPCVEAKEFNLVRNMLI 300

BLAST of Cla97C05G100120 vs. NCBI nr
Match: XP_038898951.1 (bifunctional nuclease 1 [Benincasa hispida] >XP_038898953.1 bifunctional nuclease 1 [Benincasa hispida] >XP_038898954.1 bifunctional nuclease 1 [Benincasa hispida] >XP_038898955.1 bifunctional nuclease 1 [Benincasa hispida])

HSP 1 Score: 559.7 bits (1441), Expect = 1.8e-155
Identity = 282/317 (88.96%), Postives = 286/317 (90.22%), Query Frame = 0

Query: 33  MGTLQGPVVCPTIRAKQAGFCSLPINRSLMPFSFRRGELWGFTGINGLNAKLPAISLKGC 92
           MG+LQGPVVCPT RAKQAGFCSLPINRSLM FS RRGELWGFTG+NGLNAKLPAISLKGC
Sbjct: 1   MGSLQGPVVCPTFRAKQAGFCSLPINRSLMAFSSRRGELWGFTGLNGLNAKLPAISLKGC 60

Query: 93  ARKCKTLRCSFSSSSDDNGSTAENFNEKDEEYVESSVVEAVEVKSGADGFVIKMRDGRHL 152
           ARKCKTLRCSFSSSSDDNGSTAENFNEKDEEYVESSVVEAVEVKSGADGFVIKMRDGRHL
Sbjct: 61  ARKCKTLRCSFSSSSDDNGSTAENFNEKDEEYVESSVVEAVEVKSGADGFVIKMRDGRHL 120

Query: 153 RCVHNNPHGGHLPDYAPHPAIVLKMEDGTGLLLPIIVLEMPSVLLMAAVRNVPIARPTMY 212
           RCVHNNPHGGHLPDYAPHPAIVLKMEDGTGLLLPIIVLEMPSVLLMAAVRNVPIARPTMY
Sbjct: 121 RCVHNNPHGGHLPDYAPHPAIVLKMEDGTGLLLPIIVLEMPSVLLMAAVRNVPIARPTMY 180

Query: 213 QVVKEMIDKMGYE----------------------IGCDTDCLSFDLRPSDAINIAVRCK 272
           QVVKEMIDKMGYE                      IGCDTDCLSFDLRPSDAINIAVRCK
Sbjct: 181 QVVKEMIDKMGYEVKLVRVTKRVHEAYFAQLYLSKIGCDTDCLSFDLRPSDAINIAVRCK 240

Query: 273 VPIQVNKYLAYSDGMRVVESGKLSTQAPATDGLLFTELDRPSGQPCVEAKEFNLVRNMLI 328
           VPIQVNKYLAYSDGMRVVESGKLS QAPATDGLLFTELDRPSGQPCVEAKEFNL+RNMLI
Sbjct: 241 VPIQVNKYLAYSDGMRVVESGKLSAQAPATDGLLFTELDRPSGQPCVEAKEFNLIRNMLI 300

BLAST of Cla97C05G100120 vs. NCBI nr
Match: KAG7037875.1 (Bifunctional nuclease 2 [Cucurbita argyrosperma subsp. argyrosperma])

HSP 1 Score: 557.0 bits (1434), Expect = 1.1e-154
Identity = 274/295 (92.88%), Postives = 281/295 (95.25%), Query Frame = 0

Query: 33  MGTLQGPVVCPTIRAKQAGFCSLPINRSLMPFSFRRGELWGFTGINGLNAKLPAISLKGC 92
           MG+LQGPVVCPT RAKQAGFCSLPI RSLM FSFRRGE WGFTGI+GLNAK PA S KGC
Sbjct: 1   MGSLQGPVVCPTFRAKQAGFCSLPIKRSLMAFSFRRGEFWGFTGIDGLNAKFPAPSRKGC 60

Query: 93  ARKCKTLRCSFSSSSDDNGSTAENFNEKDEEYVESSVVEAVEVKSGADGFVIKMRDGRHL 152
           ARKC T+RCSFSSSSDDNGSTAENFNEKDEEYVESSVVEAVEVKSGADGF+IKMRDGRHL
Sbjct: 61  ARKCTTVRCSFSSSSDDNGSTAENFNEKDEEYVESSVVEAVEVKSGADGFMIKMRDGRHL 120

Query: 153 RCVHNNPHGGHLPDYAPHPAIVLKMEDGTGLLLPIIVLEMPSVLLMAAVRNVPIARPTMY 212
           RCVHNNPHGGHLPDYAPHPAIVLKMEDGTGLLLPIIVLEMPSVLLMAAVRNVPIARPTMY
Sbjct: 121 RCVHNNPHGGHLPDYAPHPAIVLKMEDGTGLLLPIIVLEMPSVLLMAAVRNVPIARPTMY 180

Query: 213 QVVKEMIDKMGYEIGCDTDCLSFDLRPSDAINIAVRCKVPIQVNKYLAYSDGMRVVESGK 272
           QVVKEMIDKMGYEIGCDTDCLSFDLRPSDAINIAVRCKVPIQVNKYLAYSDGMRVVE+GK
Sbjct: 181 QVVKEMIDKMGYEIGCDTDCLSFDLRPSDAINIAVRCKVPIQVNKYLAYSDGMRVVETGK 240

Query: 273 LSTQAPATDGLLFTELDRPSGQPCVEAKEFNLVRNMLIAAVEERYRDAVTPGDSI 328
           LSTQ PATDGLLFTELDRPSGQPCVEAKEF+LVRNMLIAAVEERYRDA    D +
Sbjct: 241 LSTQTPATDGLLFTELDRPSGQPCVEAKEFDLVRNMLIAAVEERYRDAAQWRDKL 295

BLAST of Cla97C05G100120 vs. NCBI nr
Match: XP_023525215.1 (bifunctional nuclease 1-like [Cucurbita pepo subsp. pepo] >XP_023525216.1 bifunctional nuclease 1-like [Cucurbita pepo subsp. pepo] >XP_023525218.1 bifunctional nuclease 1-like [Cucurbita pepo subsp. pepo] >XP_023525219.1 bifunctional nuclease 1-like [Cucurbita pepo subsp. pepo])

HSP 1 Score: 548.9 bits (1413), Expect = 3.1e-152
Identity = 276/317 (87.07%), Postives = 282/317 (88.96%), Query Frame = 0

Query: 33  MGTLQGPVVCPTIRAKQAGFCSLPINRSLMPFSFRRGELWGFTGINGLNAKLPAISLKGC 92
           MG+LQGPVVCPT RAKQAGFCSLPI RSLM FSFRRGE WGFTGINGLNAK PAIS KGC
Sbjct: 1   MGSLQGPVVCPTFRAKQAGFCSLPIKRSLMAFSFRRGEFWGFTGINGLNAKFPAISRKGC 60

Query: 93  ARKCKTLRCSFSSSSDDNGSTAENFNEKDEEYVESSVVEAVEVKSGADGFVIKMRDGRHL 152
           ARKC T+RCSFSSSSDDNGSTAENFNEKDEEYVESSVVEAVEVKSGADGF+IKMRDGRHL
Sbjct: 61  ARKCTTVRCSFSSSSDDNGSTAENFNEKDEEYVESSVVEAVEVKSGADGFMIKMRDGRHL 120

Query: 153 RCVHNNPHGGHLPDYAPHPAIVLKMEDGTGLLLPIIVLEMPSVLLMAAVRNVPIARPTMY 212
           RCVHNNPHGGHLPDYAPHPAIVLKMEDGTGLLLPIIVLEMPSVLLMAAVRNVPIARPTMY
Sbjct: 121 RCVHNNPHGGHLPDYAPHPAIVLKMEDGTGLLLPIIVLEMPSVLLMAAVRNVPIARPTMY 180

Query: 213 QVVKEMIDKMGYE----------------------IGCDTDCLSFDLRPSDAINIAVRCK 272
           QVVKEMIDKMGYE                      IGCDTDCLSFDLRPSDAINIAVRCK
Sbjct: 181 QVVKEMIDKMGYEVKLVRVTKRVHEAYFAQLYLSKIGCDTDCLSFDLRPSDAINIAVRCK 240

Query: 273 VPIQVNKYLAYSDGMRVVESGKLSTQAPATDGLLFTELDRPSGQPCVEAKEFNLVRNMLI 328
           VPIQVNKYLAYSDGMRVVE+GKLSTQ PATDGLLFTELDRPSGQPCVEAKEF+LVRNMLI
Sbjct: 241 VPIQVNKYLAYSDGMRVVETGKLSTQTPATDGLLFTELDRPSGQPCVEAKEFDLVRNMLI 300

BLAST of Cla97C05G100120 vs. NCBI nr
Match: XP_004147851.1 (bifunctional nuclease 1 [Cucumis sativus] >XP_011652430.1 bifunctional nuclease 1 [Cucumis sativus] >KGN59990.1 hypothetical protein Csa_001875 [Cucumis sativus])

HSP 1 Score: 548.1 bits (1411), Expect = 5.3e-152
Identity = 278/317 (87.70%), Postives = 285/317 (89.91%), Query Frame = 0

Query: 33  MGTLQGPVVCPTIRAKQAGFCSLPINRSLMPFSFRRGELWGFTGINGLNAKLPAISLKGC 92
           MG+LQGPVVCPTI AKQAGFCSLPINRSLM FSFRRG+LWGFTGINGLNAKLPAISLK C
Sbjct: 1   MGSLQGPVVCPTICAKQAGFCSLPINRSLMAFSFRRGQLWGFTGINGLNAKLPAISLKRC 60

Query: 93  ARKCKTLRCSFSSSSDDNGSTAENFNEKDEEYVESSVVEAVEVKSGADGFVIKMRDGRHL 152
            RKCKTLRCSFSSSSDDNGSTAENFNEK+EEYVESSVVEAVEVKSGADGFVIKMRDGRHL
Sbjct: 61  TRKCKTLRCSFSSSSDDNGSTAENFNEKNEEYVESSVVEAVEVKSGADGFVIKMRDGRHL 120

Query: 153 RCVHNNPHGGHLPDYAPHPAIVLKMEDGTGLLLPIIVLEMPSVLLMAAVRNVPIARPTMY 212
           RCVHNNPHGGHLPDYAPHPAIVLKMEDGTGLLLPIIVLEMPSVLLMAAVRNVPIARPTMY
Sbjct: 121 RCVHNNPHGGHLPDYAPHPAIVLKMEDGTGLLLPIIVLEMPSVLLMAAVRNVPIARPTMY 180

Query: 213 QVVKEMIDKMGYE----------------------IGCDTDCLSFDLRPSDAINIAVRCK 272
           QVVKEMIDKMGYE                      IGC+TDCLSFDLRPSDAINIAVRC+
Sbjct: 181 QVVKEMIDKMGYEVKLVRVTKRVHEAYFAQLYLSKIGCETDCLSFDLRPSDAINIAVRCQ 240

Query: 273 VPIQVNKYLAYSDGMRVVESGKLSTQAPATDGLLFTELDRPSGQPCVEAKEFNLVRNMLI 328
           VPIQVNKYLA+SDGMRVVESGKLSTQA ATDGLLFTELDRPSGQPCVEAKEFNLVRNMLI
Sbjct: 241 VPIQVNKYLAFSDGMRVVESGKLSTQALATDGLLFTELDRPSGQPCVEAKEFNLVRNMLI 300

BLAST of Cla97C05G100120 vs. ExPASy Swiss-Prot
Match: Q93VH2 (Bifunctional nuclease 2 OS=Arabidopsis thaliana OX=3702 GN=BBD2 PE=2 SV=1)

HSP 1 Score: 387.5 bits (994), Expect = 1.6e-106
Identity = 199/317 (62.78%), Postives = 239/317 (75.39%), Query Frame = 0

Query: 33  MGTLQGPVVCPTIRAKQAGFCSLPINRSLMPFSFRRGELWGFTGINGLNAKLPAISLKGC 92
           M +LQ PVVCP++R +Q G  +L +N S+      R + WG    N   ++   ++L+  
Sbjct: 1   MRSLQAPVVCPSVRPRQLGVSALLVNCSVSKTRSLRKQFWGNQTKND-KSQAATVNLRLH 60

Query: 93  ARKCKTLRCSFSSSSDDNGSTAENFNEKDEEYVESSVVEAVEVKSGADGFVIKMRDGRHL 152
            R+ K+++C FSS SD  GSTAENFNE DE+YV+SSV+EAVEVKSG DGF++KM+DGR L
Sbjct: 61  LRRYKSIKCLFSSHSDGTGSTAENFNENDEDYVKSSVLEAVEVKSGPDGFMVKMKDGRQL 120

Query: 153 RCVHNNPHGGHLPDYAPHPAIVLKMEDGTGLLLPIIVLEMPSVLLMAAVRNVPIARPTMY 212
           RCVHNNP GG+LP+YAPH AIVLKMEDGTGLLLPIIVLEMPSVLLMAA+ NV IARPTMY
Sbjct: 121 RCVHNNPQGGNLPNYAPHSAIVLKMEDGTGLLLPIIVLEMPSVLLMAAMTNVQIARPTMY 180

Query: 213 QVVKEMIDKMGYE----------------------IGCDTDCLSFDLRPSDAINIAVRCK 272
           QVVK+M+DKMGYE                      +G  +DC+SFDLRPSDAINIAVRCK
Sbjct: 181 QVVKDMVDKMGYEVRLVRVTTRVHEAYFAELYLSKVGDKSDCVSFDLRPSDAINIAVRCK 240

Query: 273 VPIQVNKYLAYSDGMRVVESGKLSTQAPATDGLLFTELDRPSGQPCVEAKEFNLVRNMLI 328
           VPIQVNKYLAYSDGMRV++SGKLS Q PA+DGLLFTELDRP+GQPC + KEF+LVRNM+ 
Sbjct: 241 VPIQVNKYLAYSDGMRVIDSGKLSKQTPASDGLLFTELDRPNGQPCFDTKEFDLVRNMMQ 300

BLAST of Cla97C05G100120 vs. ExPASy Swiss-Prot
Match: Q9FWS6 (Bifunctional nuclease 1 OS=Arabidopsis thaliana OX=3702 GN=BBD1 PE=2 SV=1)

HSP 1 Score: 386.3 bits (991), Expect = 3.5e-106
Identity = 204/320 (63.75%), Postives = 238/320 (74.38%), Query Frame = 0

Query: 33  MGTLQGPVVCPTIRAKQAGFCSLPIN-RSLMPFSFRRGELWGFTGINGLNAK--LPAISL 92
           M ++Q PVVCP IR +Q G C+  +N   L P    R + WG     G+ ++     I+L
Sbjct: 1   MRSVQAPVVCPAIRPRQVGACASLVNYTGLKP----RSQFWG-NRTKGVKSQGTTTTITL 60

Query: 93  KGCARKCKTLRCSFSSSSDDNGSTAENFNEKDEEYVESSVVEAVEVKSGADGFVIKMRDG 152
           + C    K+++C FSS SD NGSTAENFNE DEEYV SSVVEAVEVKSGADGF++KMRDG
Sbjct: 61  RLCN---KSIKCVFSSHSDGNGSTAENFNENDEEYVNSSVVEAVEVKSGADGFMVKMRDG 120

Query: 153 RHLRCVHNNPHGGHLPDYAPHPAIVLKMEDGTGLLLPIIVLEMPSVLLMAAVRNVPIARP 212
           R LRCVHNNP GGHLPDYAPHPAIVLKMEDGTGLLLPIIVLEMPSVLLMAA+ NV IARP
Sbjct: 121 RQLRCVHNNPQGGHLPDYAPHPAIVLKMEDGTGLLLPIIVLEMPSVLLMAAMTNVQIARP 180

Query: 213 TMYQVVKEMIDKMGYE----------------------IGCDTDCLSFDLRPSDAINIAV 272
           TMYQVVKEM+DKMGYE                      +G  ++C+SFDLRPSDAINIAV
Sbjct: 181 TMYQVVKEMVDKMGYEVRLVRVTKRVHEAYFAQLFLSKVGNASECVSFDLRPSDAINIAV 240

Query: 273 RCKVPIQVNKYLAYSDGMRVVESGKLSTQAPATDGLLFTELDRPSGQPCVEAKEFNLVRN 328
           RCK+PIQVNKYLAYSDGMRV+ESGK+ST APA+DGLLFTE DRP+GQ C++ KEFN++  
Sbjct: 241 RCKIPIQVNKYLAYSDGMRVIESGKISTPAPASDGLLFTEQDRPNGQACLDTKEFNILSK 300

BLAST of Cla97C05G100120 vs. ExPASy Swiss-Prot
Match: Q6YZM6 (Bifunctional nuclease 2 OS=Oryza sativa subsp. japonica OX=39947 GN=BBD2 PE=2 SV=1)

HSP 1 Score: 317.4 bits (812), Expect = 2.0e-85
Identity = 164/254 (64.57%), Postives = 200/254 (78.74%), Query Frame = 0

Query: 102 SFSSSSDDNGSTAENFNEKDEEYVESSVVEAVEVKSGADGFVIKMRDGRHLRCVHNNPHG 161
           SFSSSSD NG  A NF+E DE+YV S+V+EAVEV+SGA+G+VIKMRDG++LRCVHNN  G
Sbjct: 67  SFSSSSDGNGYMAGNFSESDEDYVNSTVLEAVEVRSGAEGYVIKMRDGKNLRCVHNNSQG 126

Query: 162 GHLPDYAPHPAIVLKMEDGTGLLLPIIVLEMPSVLLMAAVRNVPIARPTMYQVVKEMIDK 221
            ++P+ AP PAIVL++EDG+  LLPIIVLEMPSVLLMAA+RNV IARPT+YQVVKEMIDK
Sbjct: 127 RNIPESAPQPAIVLRIEDGSETLLPIIVLEMPSVLLMAAIRNVHIARPTIYQVVKEMIDK 186

Query: 222 MGYE----------------------IGCDTDCLSFDLRPSDAINIAVRCKVPIQVNKYL 281
           MGYE                      +G  T+ ++FDLRPSDAINIAVRCKVPIQV++ L
Sbjct: 187 MGYEVKLVRINKRIQEAYCAELFLTKVGDHTESITFDLRPSDAINIAVRCKVPIQVHRSL 246

Query: 282 AYSDGMRVVESGKLSTQAPATDGLLFTELDRPSGQPCVEAKEFNLVRNMLIAAVEERYRD 334
           AYSDG+R VE  +++  A  +DGLLFTELDRP GQPCVEA+EF L+RNMLIAAVEERY+D
Sbjct: 247 AYSDGIRSVEPARMAIAAGMSDGLLFTELDRPDGQPCVEAQEFGLIRNMLIAAVEERYKD 306

BLAST of Cla97C05G100120 vs. ExPASy Swiss-Prot
Match: Q5N8J3 (Bifunctional nuclease 1 OS=Oryza sativa subsp. japonica OX=39947 GN=BBD1 PE=2 SV=1)

HSP 1 Score: 259.2 bits (661), Expect = 6.5e-68
Identity = 136/255 (53.33%), Postives = 181/255 (70.98%), Query Frame = 0

Query: 101 CSFSSSSDDNGSTAENFNEKDEEYVESSVVEAVEVKSGADGFVIKMRDGRHLRCVHNNPH 160
           CS+ SSSD +G+ A +++   EE+V SSV+EAVE++S +DGFVIKMRDG++LRCV NNP 
Sbjct: 73  CSYGSSSDGDGAAAADYDASGEEFVNSSVMEAVELRSVSDGFVIKMRDGKNLRCVQNNPR 132

Query: 161 GGHLPDYAPHPAIVLKMEDGTGLLLPIIVLEMPSVLLMAAVRNVPIARPTMYQVVKEMID 220
              L D APH AIVLKMEDG+ LLLPIIV+E PS++L+AA+RN+ I RPT+Y VVKEM +
Sbjct: 133 VLRLRDSAPHHAIVLKMEDGSDLLLPIIVMETPSIMLLAALRNIRIPRPTIYNVVKEMTE 192

Query: 221 KMGY----------------------EIGCDTDCLSFDLRPSDAINIAVRCKVPIQVNKY 280
           +MGY                      +IG + + +S DL+PSDAINIA RCKVPIQVN+ 
Sbjct: 193 RMGYAVRLVRITEMVHDAYYSRLYLAKIGNEEETISLDLKPSDAINIAFRCKVPIQVNRR 252

Query: 281 LAYSDGMRVVESGKLSTQAPATDGLLFTELDRPSGQPCVEAKEFNLVRNMLIAAVEERYR 334
           +AY++G++VV+    S    ++D   +T LDRP  QPC EA+EF+LVRNML+AAVEERY+
Sbjct: 253 IAYNNGLKVVQPTP-SESYVSSDQFQYTRLDRPDDQPCFEAQEFDLVRNMLVAAVEERYK 312

BLAST of Cla97C05G100120 vs. ExPASy Swiss-Prot
Match: Q09LL3 (Bifunctional nuclease OS=Oryza minuta OX=63629 GN=BBD PE=2 SV=1)

HSP 1 Score: 259.2 bits (661), Expect = 6.5e-68
Identity = 136/255 (53.33%), Postives = 181/255 (70.98%), Query Frame = 0

Query: 101 CSFSSSSDDNGSTAENFNEKDEEYVESSVVEAVEVKSGADGFVIKMRDGRHLRCVHNNPH 160
           CS+ SSSD +G+ A +++   EE+V SSV+EAVE++S +DGFVIKMRDG++LRCV NNP 
Sbjct: 73  CSYGSSSDGDGAAAADYDASGEEFVNSSVMEAVELRSVSDGFVIKMRDGKNLRCVQNNPR 132

Query: 161 GGHLPDYAPHPAIVLKMEDGTGLLLPIIVLEMPSVLLMAAVRNVPIARPTMYQVVKEMID 220
              L D APH AIVLKMEDG+ LLLPIIV+E PS++L+AA+RN+ I RPT+Y VVKEM +
Sbjct: 133 VLRLRDSAPHHAIVLKMEDGSDLLLPIIVMETPSIMLLAALRNIRIPRPTIYNVVKEMTE 192

Query: 221 KMGY----------------------EIGCDTDCLSFDLRPSDAINIAVRCKVPIQVNKY 280
           +MGY                      +IG + + +S DL+PSDAINIA RCKVPIQVN+ 
Sbjct: 193 RMGYAVRLVRITEMVHDAYYSRLYLAKIGNEEETISLDLKPSDAINIAFRCKVPIQVNRR 252

Query: 281 LAYSDGMRVVESGKLSTQAPATDGLLFTELDRPSGQPCVEAKEFNLVRNMLIAAVEERYR 334
           +AY++G++VV+    S    ++D   +T LDRP  QPC EA+EF+LVRNML+AAVEERY+
Sbjct: 253 IAYNNGLKVVQPTP-SESYVSSDQFQYTRLDRPDDQPCFEAQEFDLVRNMLVAAVEERYK 312

BLAST of Cla97C05G100120 vs. ExPASy TrEMBL
Match: A0A1S3CRI0 (bifunctional nuclease 1 OS=Cucumis melo OX=3656 GN=LOC103503917 PE=3 SV=1)

HSP 1 Score: 565.5 bits (1456), Expect = 1.6e-157
Identity = 285/317 (89.91%), Postives = 289/317 (91.17%), Query Frame = 0

Query: 33  MGTLQGPVVCPTIRAKQAGFCSLPINRSLMPFSFRRGELWGFTGINGLNAKLPAISLKGC 92
           MG+LQGPVVCPTIRAKQAGFCSLPINRSLM FSFRRGELWGFTGINGLNAKLPAISLKGC
Sbjct: 1   MGSLQGPVVCPTIRAKQAGFCSLPINRSLMAFSFRRGELWGFTGINGLNAKLPAISLKGC 60

Query: 93  ARKCKTLRCSFSSSSDDNGSTAENFNEKDEEYVESSVVEAVEVKSGADGFVIKMRDGRHL 152
           ARKCKTLRCSFSSSSDDNGSTAENFNEKDEEYVESSVVEAVEVKSGADGFVIKMRDGRHL
Sbjct: 61  ARKCKTLRCSFSSSSDDNGSTAENFNEKDEEYVESSVVEAVEVKSGADGFVIKMRDGRHL 120

Query: 153 RCVHNNPHGGHLPDYAPHPAIVLKMEDGTGLLLPIIVLEMPSVLLMAAVRNVPIARPTMY 212
           RCVHNNPHGGHLPDYAPHPAIVLKMEDGTGLLLPIIVLEMPSVLLMAAVRNVPIARPTMY
Sbjct: 121 RCVHNNPHGGHLPDYAPHPAIVLKMEDGTGLLLPIIVLEMPSVLLMAAVRNVPIARPTMY 180

Query: 213 QVVKEMIDKMGYE----------------------IGCDTDCLSFDLRPSDAINIAVRCK 272
           QVVKEM+DKMGYE                      IGC+TDCLSFDLRPSDAINIAVRCK
Sbjct: 181 QVVKEMVDKMGYEVKLVRVTKRVHEAYFAQLYLSKIGCETDCLSFDLRPSDAINIAVRCK 240

Query: 273 VPIQVNKYLAYSDGMRVVESGKLSTQAPATDGLLFTELDRPSGQPCVEAKEFNLVRNMLI 328
           VPIQVNKYLAYSDGMRVVESGKLSTQAPATDGLLFTELDRPSGQPCVEAKEFNLVRNMLI
Sbjct: 241 VPIQVNKYLAYSDGMRVVESGKLSTQAPATDGLLFTELDRPSGQPCVEAKEFNLVRNMLI 300

BLAST of Cla97C05G100120 vs. ExPASy TrEMBL
Match: A0A5D3D874 (Bifunctional nuclease 1 OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold242G001050 PE=3 SV=1)

HSP 1 Score: 565.5 bits (1456), Expect = 1.6e-157
Identity = 285/317 (89.91%), Postives = 289/317 (91.17%), Query Frame = 0

Query: 33  MGTLQGPVVCPTIRAKQAGFCSLPINRSLMPFSFRRGELWGFTGINGLNAKLPAISLKGC 92
           MG+LQGPVVCPTIRAKQAGFCSLPINRSLM FSFRRGELWGFTGINGLNAKLPAISLKGC
Sbjct: 1   MGSLQGPVVCPTIRAKQAGFCSLPINRSLMAFSFRRGELWGFTGINGLNAKLPAISLKGC 60

Query: 93  ARKCKTLRCSFSSSSDDNGSTAENFNEKDEEYVESSVVEAVEVKSGADGFVIKMRDGRHL 152
           ARKCKTLRCSFSSSSDDNGSTAENFNEKDEEYVESSVVEAVEVKSGADGFVIKMRDGRHL
Sbjct: 61  ARKCKTLRCSFSSSSDDNGSTAENFNEKDEEYVESSVVEAVEVKSGADGFVIKMRDGRHL 120

Query: 153 RCVHNNPHGGHLPDYAPHPAIVLKMEDGTGLLLPIIVLEMPSVLLMAAVRNVPIARPTMY 212
           RCVHNNPHGGHLPDYAPHPAIVLKMEDGTGLLLPIIVLEMPSVLLMAAVRNVPIARPTMY
Sbjct: 121 RCVHNNPHGGHLPDYAPHPAIVLKMEDGTGLLLPIIVLEMPSVLLMAAVRNVPIARPTMY 180

Query: 213 QVVKEMIDKMGYE----------------------IGCDTDCLSFDLRPSDAINIAVRCK 272
           QVVKEM+DKMGYE                      IGC+TDCLSFDLRPSDAINIAVRCK
Sbjct: 181 QVVKEMVDKMGYEVKLVRVTKRVHEAYFAQLYLSKIGCETDCLSFDLRPSDAINIAVRCK 240

Query: 273 VPIQVNKYLAYSDGMRVVESGKLSTQAPATDGLLFTELDRPSGQPCVEAKEFNLVRNMLI 328
           VPIQVNKYLAYSDGMRVVESGKLSTQAPATDGLLFTELDRPSGQPCVEAKEFNLVRNMLI
Sbjct: 241 VPIQVNKYLAYSDGMRVVESGKLSTQAPATDGLLFTELDRPSGQPCVEAKEFNLVRNMLI 300

BLAST of Cla97C05G100120 vs. ExPASy TrEMBL
Match: A0A0A0LDD4 (BFN domain-containing protein OS=Cucumis sativus OX=3659 GN=Csa_3G860260 PE=3 SV=1)

HSP 1 Score: 548.1 bits (1411), Expect = 2.6e-152
Identity = 278/317 (87.70%), Postives = 285/317 (89.91%), Query Frame = 0

Query: 33  MGTLQGPVVCPTIRAKQAGFCSLPINRSLMPFSFRRGELWGFTGINGLNAKLPAISLKGC 92
           MG+LQGPVVCPTI AKQAGFCSLPINRSLM FSFRRG+LWGFTGINGLNAKLPAISLK C
Sbjct: 1   MGSLQGPVVCPTICAKQAGFCSLPINRSLMAFSFRRGQLWGFTGINGLNAKLPAISLKRC 60

Query: 93  ARKCKTLRCSFSSSSDDNGSTAENFNEKDEEYVESSVVEAVEVKSGADGFVIKMRDGRHL 152
            RKCKTLRCSFSSSSDDNGSTAENFNEK+EEYVESSVVEAVEVKSGADGFVIKMRDGRHL
Sbjct: 61  TRKCKTLRCSFSSSSDDNGSTAENFNEKNEEYVESSVVEAVEVKSGADGFVIKMRDGRHL 120

Query: 153 RCVHNNPHGGHLPDYAPHPAIVLKMEDGTGLLLPIIVLEMPSVLLMAAVRNVPIARPTMY 212
           RCVHNNPHGGHLPDYAPHPAIVLKMEDGTGLLLPIIVLEMPSVLLMAAVRNVPIARPTMY
Sbjct: 121 RCVHNNPHGGHLPDYAPHPAIVLKMEDGTGLLLPIIVLEMPSVLLMAAVRNVPIARPTMY 180

Query: 213 QVVKEMIDKMGYE----------------------IGCDTDCLSFDLRPSDAINIAVRCK 272
           QVVKEMIDKMGYE                      IGC+TDCLSFDLRPSDAINIAVRC+
Sbjct: 181 QVVKEMIDKMGYEVKLVRVTKRVHEAYFAQLYLSKIGCETDCLSFDLRPSDAINIAVRCQ 240

Query: 273 VPIQVNKYLAYSDGMRVVESGKLSTQAPATDGLLFTELDRPSGQPCVEAKEFNLVRNMLI 328
           VPIQVNKYLA+SDGMRVVESGKLSTQA ATDGLLFTELDRPSGQPCVEAKEFNLVRNMLI
Sbjct: 241 VPIQVNKYLAFSDGMRVVESGKLSTQALATDGLLFTELDRPSGQPCVEAKEFNLVRNMLI 300

BLAST of Cla97C05G100120 vs. ExPASy TrEMBL
Match: A0A6J1FM73 (bifunctional nuclease 1-like OS=Cucurbita moschata OX=3662 GN=LOC111446486 PE=3 SV=1)

HSP 1 Score: 544.3 bits (1401), Expect = 3.7e-151
Identity = 274/317 (86.44%), Postives = 280/317 (88.33%), Query Frame = 0

Query: 33  MGTLQGPVVCPTIRAKQAGFCSLPINRSLMPFSFRRGELWGFTGINGLNAKLPAISLKGC 92
           MG+LQGPVVCPT  AKQAGFCSLPI RSLM FSFRRGE WGFTGINGLNAK PA S KGC
Sbjct: 1   MGSLQGPVVCPTFHAKQAGFCSLPIKRSLMAFSFRRGEFWGFTGINGLNAKFPAPSRKGC 60

Query: 93  ARKCKTLRCSFSSSSDDNGSTAENFNEKDEEYVESSVVEAVEVKSGADGFVIKMRDGRHL 152
           ARKC T+RCSFSSSSDDNGSTAENFNEKDEEYVESSVVEAVEVKSGADGF+IKMRDGRHL
Sbjct: 61  ARKCTTVRCSFSSSSDDNGSTAENFNEKDEEYVESSVVEAVEVKSGADGFMIKMRDGRHL 120

Query: 153 RCVHNNPHGGHLPDYAPHPAIVLKMEDGTGLLLPIIVLEMPSVLLMAAVRNVPIARPTMY 212
           RCVHNNPHGGHLPDYAPHPAIVLKMEDGTGLLLPIIVLEMPSVLLMAAVRNVPIARPTMY
Sbjct: 121 RCVHNNPHGGHLPDYAPHPAIVLKMEDGTGLLLPIIVLEMPSVLLMAAVRNVPIARPTMY 180

Query: 213 QVVKEMIDKMGYE----------------------IGCDTDCLSFDLRPSDAINIAVRCK 272
           QVVKEMIDKMGYE                      IGCDTDCLSFDLRPSDAINIAVRCK
Sbjct: 181 QVVKEMIDKMGYEVKLVRVTKRVHEAYFAQLYLSKIGCDTDCLSFDLRPSDAINIAVRCK 240

Query: 273 VPIQVNKYLAYSDGMRVVESGKLSTQAPATDGLLFTELDRPSGQPCVEAKEFNLVRNMLI 328
           VPIQVNKYLAYSDGMRVVE+GKLSTQ PATDGLLFTELDRPSGQPCVEAKEF+LVRNMLI
Sbjct: 241 VPIQVNKYLAYSDGMRVVETGKLSTQTPATDGLLFTELDRPSGQPCVEAKEFDLVRNMLI 300

BLAST of Cla97C05G100120 vs. ExPASy TrEMBL
Match: A0A6J1IXM9 (bifunctional nuclease 1-like OS=Cucurbita maxima OX=3661 GN=LOC111480857 PE=3 SV=1)

HSP 1 Score: 541.2 bits (1393), Expect = 3.1e-150
Identity = 271/317 (85.49%), Postives = 280/317 (88.33%), Query Frame = 0

Query: 33  MGTLQGPVVCPTIRAKQAGFCSLPINRSLMPFSFRRGELWGFTGINGLNAKLPAISLKGC 92
           MG+LQGPVVCPT RAKQAGFCSLPI R LM FSFRRGE WGFTGINGLNAK PA+S KGC
Sbjct: 1   MGSLQGPVVCPTFRAKQAGFCSLPIKRPLMAFSFRRGEFWGFTGINGLNAKFPAMSRKGC 60

Query: 93  ARKCKTLRCSFSSSSDDNGSTAENFNEKDEEYVESSVVEAVEVKSGADGFVIKMRDGRHL 152
           ARKC T+RCSFSSSSDDNGSTAENFN+KDEEYVESSVVEAVEVKSGADGF+IKMRDGRHL
Sbjct: 61  ARKCTTVRCSFSSSSDDNGSTAENFNDKDEEYVESSVVEAVEVKSGADGFMIKMRDGRHL 120

Query: 153 RCVHNNPHGGHLPDYAPHPAIVLKMEDGTGLLLPIIVLEMPSVLLMAAVRNVPIARPTMY 212
           RCVHNNPHGGHLPDYAPHPAIVLKMEDGTGLLLPIIVLEMPSVLLMAAVRNVPIARPTMY
Sbjct: 121 RCVHNNPHGGHLPDYAPHPAIVLKMEDGTGLLLPIIVLEMPSVLLMAAVRNVPIARPTMY 180

Query: 213 QVVKEMIDKMGYE----------------------IGCDTDCLSFDLRPSDAINIAVRCK 272
           QVVKEMIDKMGYE                      IGC+ DCLSFDLRPSDAINIAVRCK
Sbjct: 181 QVVKEMIDKMGYEVKLVRVTKRVHEAYFAQLYLSKIGCENDCLSFDLRPSDAINIAVRCK 240

Query: 273 VPIQVNKYLAYSDGMRVVESGKLSTQAPATDGLLFTELDRPSGQPCVEAKEFNLVRNMLI 328
           VPIQVNKYLAYSDGMRVVE+GKLSTQ PATDGLLFTELDRPSGQPCVEAKEF+LVRNMLI
Sbjct: 241 VPIQVNKYLAYSDGMRVVETGKLSTQTPATDGLLFTELDRPSGQPCVEAKEFDLVRNMLI 300

BLAST of Cla97C05G100120 vs. TAIR 10
Match: AT1G19660.1 (Wound-responsive family protein )

HSP 1 Score: 387.5 bits (994), Expect = 1.1e-107
Identity = 199/317 (62.78%), Postives = 239/317 (75.39%), Query Frame = 0

Query: 33  MGTLQGPVVCPTIRAKQAGFCSLPINRSLMPFSFRRGELWGFTGINGLNAKLPAISLKGC 92
           M +LQ PVVCP++R +Q G  +L +N S+      R + WG    N   ++   ++L+  
Sbjct: 1   MRSLQAPVVCPSVRPRQLGVSALLVNCSVSKTRSLRKQFWGNQTKND-KSQAATVNLRLH 60

Query: 93  ARKCKTLRCSFSSSSDDNGSTAENFNEKDEEYVESSVVEAVEVKSGADGFVIKMRDGRHL 152
            R+ K+++C FSS SD  GSTAENFNE DE+YV+SSV+EAVEVKSG DGF++KM+DGR L
Sbjct: 61  LRRYKSIKCLFSSHSDGTGSTAENFNENDEDYVKSSVLEAVEVKSGPDGFMVKMKDGRQL 120

Query: 153 RCVHNNPHGGHLPDYAPHPAIVLKMEDGTGLLLPIIVLEMPSVLLMAAVRNVPIARPTMY 212
           RCVHNNP GG+LP+YAPH AIVLKMEDGTGLLLPIIVLEMPSVLLMAA+ NV IARPTMY
Sbjct: 121 RCVHNNPQGGNLPNYAPHSAIVLKMEDGTGLLLPIIVLEMPSVLLMAAMTNVQIARPTMY 180

Query: 213 QVVKEMIDKMGYE----------------------IGCDTDCLSFDLRPSDAINIAVRCK 272
           QVVK+M+DKMGYE                      +G  +DC+SFDLRPSDAINIAVRCK
Sbjct: 181 QVVKDMVDKMGYEVRLVRVTTRVHEAYFAELYLSKVGDKSDCVSFDLRPSDAINIAVRCK 240

Query: 273 VPIQVNKYLAYSDGMRVVESGKLSTQAPATDGLLFTELDRPSGQPCVEAKEFNLVRNMLI 328
           VPIQVNKYLAYSDGMRV++SGKLS Q PA+DGLLFTELDRP+GQPC + KEF+LVRNM+ 
Sbjct: 241 VPIQVNKYLAYSDGMRVIDSGKLSKQTPASDGLLFTELDRPNGQPCFDTKEFDLVRNMMQ 300

BLAST of Cla97C05G100120 vs. TAIR 10
Match: AT1G19660.2 (Wound-responsive family protein )

HSP 1 Score: 387.5 bits (994), Expect = 1.1e-107
Identity = 199/317 (62.78%), Postives = 239/317 (75.39%), Query Frame = 0

Query: 33  MGTLQGPVVCPTIRAKQAGFCSLPINRSLMPFSFRRGELWGFTGINGLNAKLPAISLKGC 92
           M +LQ PVVCP++R +Q G  +L +N S+      R + WG    N   ++   ++L+  
Sbjct: 1   MRSLQAPVVCPSVRPRQLGVSALLVNCSVSKTRSLRKQFWGNQTKND-KSQAATVNLRLH 60

Query: 93  ARKCKTLRCSFSSSSDDNGSTAENFNEKDEEYVESSVVEAVEVKSGADGFVIKMRDGRHL 152
            R+ K+++C FSS SD  GSTAENFNE DE+YV+SSV+EAVEVKSG DGF++KM+DGR L
Sbjct: 61  LRRYKSIKCLFSSHSDGTGSTAENFNENDEDYVKSSVLEAVEVKSGPDGFMVKMKDGRQL 120

Query: 153 RCVHNNPHGGHLPDYAPHPAIVLKMEDGTGLLLPIIVLEMPSVLLMAAVRNVPIARPTMY 212
           RCVHNNP GG+LP+YAPH AIVLKMEDGTGLLLPIIVLEMPSVLLMAA+ NV IARPTMY
Sbjct: 121 RCVHNNPQGGNLPNYAPHSAIVLKMEDGTGLLLPIIVLEMPSVLLMAAMTNVQIARPTMY 180

Query: 213 QVVKEMIDKMGYE----------------------IGCDTDCLSFDLRPSDAINIAVRCK 272
           QVVK+M+DKMGYE                      +G  +DC+SFDLRPSDAINIAVRCK
Sbjct: 181 QVVKDMVDKMGYEVRLVRVTTRVHEAYFAELYLSKVGDKSDCVSFDLRPSDAINIAVRCK 240

Query: 273 VPIQVNKYLAYSDGMRVVESGKLSTQAPATDGLLFTELDRPSGQPCVEAKEFNLVRNMLI 328
           VPIQVNKYLAYSDGMRV++SGKLS Q PA+DGLLFTELDRP+GQPC + KEF+LVRNM+ 
Sbjct: 241 VPIQVNKYLAYSDGMRVIDSGKLSKQTPASDGLLFTELDRPNGQPCFDTKEFDLVRNMMQ 300

BLAST of Cla97C05G100120 vs. TAIR 10
Match: AT1G75380.1 (bifunctional nuclease in basal defense response 1 )

HSP 1 Score: 386.3 bits (991), Expect = 2.5e-107
Identity = 204/320 (63.75%), Postives = 238/320 (74.38%), Query Frame = 0

Query: 33  MGTLQGPVVCPTIRAKQAGFCSLPIN-RSLMPFSFRRGELWGFTGINGLNAK--LPAISL 92
           M ++Q PVVCP IR +Q G C+  +N   L P    R + WG     G+ ++     I+L
Sbjct: 1   MRSVQAPVVCPAIRPRQVGACASLVNYTGLKP----RSQFWG-NRTKGVKSQGTTTTITL 60

Query: 93  KGCARKCKTLRCSFSSSSDDNGSTAENFNEKDEEYVESSVVEAVEVKSGADGFVIKMRDG 152
           + C    K+++C FSS SD NGSTAENFNE DEEYV SSVVEAVEVKSGADGF++KMRDG
Sbjct: 61  RLCN---KSIKCVFSSHSDGNGSTAENFNENDEEYVNSSVVEAVEVKSGADGFMVKMRDG 120

Query: 153 RHLRCVHNNPHGGHLPDYAPHPAIVLKMEDGTGLLLPIIVLEMPSVLLMAAVRNVPIARP 212
           R LRCVHNNP GGHLPDYAPHPAIVLKMEDGTGLLLPIIVLEMPSVLLMAA+ NV IARP
Sbjct: 121 RQLRCVHNNPQGGHLPDYAPHPAIVLKMEDGTGLLLPIIVLEMPSVLLMAAMTNVQIARP 180

Query: 213 TMYQVVKEMIDKMGYE----------------------IGCDTDCLSFDLRPSDAINIAV 272
           TMYQVVKEM+DKMGYE                      +G  ++C+SFDLRPSDAINIAV
Sbjct: 181 TMYQVVKEMVDKMGYEVRLVRVTKRVHEAYFAQLFLSKVGNASECVSFDLRPSDAINIAV 240

Query: 273 RCKVPIQVNKYLAYSDGMRVVESGKLSTQAPATDGLLFTELDRPSGQPCVEAKEFNLVRN 328
           RCK+PIQVNKYLAYSDGMRV+ESGK+ST APA+DGLLFTE DRP+GQ C++ KEFN++  
Sbjct: 241 RCKIPIQVNKYLAYSDGMRVIESGKISTPAPASDGLLFTEQDRPNGQACLDTKEFNILSK 300

BLAST of Cla97C05G100120 vs. TAIR 10
Match: AT1G75380.2 (bifunctional nuclease in basal defense response 1 )

HSP 1 Score: 386.3 bits (991), Expect = 2.5e-107
Identity = 204/320 (63.75%), Postives = 238/320 (74.38%), Query Frame = 0

Query: 33  MGTLQGPVVCPTIRAKQAGFCSLPIN-RSLMPFSFRRGELWGFTGINGLNAK--LPAISL 92
           M ++Q PVVCP IR +Q G C+  +N   L P    R + WG     G+ ++     I+L
Sbjct: 1   MRSVQAPVVCPAIRPRQVGACASLVNYTGLKP----RSQFWG-NRTKGVKSQGTTTTITL 60

Query: 93  KGCARKCKTLRCSFSSSSDDNGSTAENFNEKDEEYVESSVVEAVEVKSGADGFVIKMRDG 152
           + C    K+++C FSS SD NGSTAENFNE DEEYV SSVVEAVEVKSGADGF++KMRDG
Sbjct: 61  RLCN---KSIKCVFSSHSDGNGSTAENFNENDEEYVNSSVVEAVEVKSGADGFMVKMRDG 120

Query: 153 RHLRCVHNNPHGGHLPDYAPHPAIVLKMEDGTGLLLPIIVLEMPSVLLMAAVRNVPIARP 212
           R LRCVHNNP GGHLPDYAPHPAIVLKMEDGTGLLLPIIVLEMPSVLLMAA+ NV IARP
Sbjct: 121 RQLRCVHNNPQGGHLPDYAPHPAIVLKMEDGTGLLLPIIVLEMPSVLLMAAMTNVQIARP 180

Query: 213 TMYQVVKEMIDKMGYE----------------------IGCDTDCLSFDLRPSDAINIAV 272
           TMYQVVKEM+DKMGYE                      +G  ++C+SFDLRPSDAINIAV
Sbjct: 181 TMYQVVKEMVDKMGYEVRLVRVTKRVHEAYFAQLFLSKVGNASECVSFDLRPSDAINIAV 240

Query: 273 RCKVPIQVNKYLAYSDGMRVVESGKLSTQAPATDGLLFTELDRPSGQPCVEAKEFNLVRN 328
           RCK+PIQVNKYLAYSDGMRV+ESGK+ST APA+DGLLFTE DRP+GQ C++ KEFN++  
Sbjct: 241 RCKIPIQVNKYLAYSDGMRVIESGKISTPAPASDGLLFTEQDRPNGQACLDTKEFNILSK 300

BLAST of Cla97C05G100120 vs. TAIR 10
Match: AT1G75380.3 (bifunctional nuclease in basal defense response 1 )

HSP 1 Score: 386.3 bits (991), Expect = 2.5e-107
Identity = 204/320 (63.75%), Postives = 238/320 (74.38%), Query Frame = 0

Query: 33  MGTLQGPVVCPTIRAKQAGFCSLPIN-RSLMPFSFRRGELWGFTGINGLNAK--LPAISL 92
           M ++Q PVVCP IR +Q G C+  +N   L P    R + WG     G+ ++     I+L
Sbjct: 1   MRSVQAPVVCPAIRPRQVGACASLVNYTGLKP----RSQFWG-NRTKGVKSQGTTTTITL 60

Query: 93  KGCARKCKTLRCSFSSSSDDNGSTAENFNEKDEEYVESSVVEAVEVKSGADGFVIKMRDG 152
           + C    K+++C FSS SD NGSTAENFNE DEEYV SSVVEAVEVKSGADGF++KMRDG
Sbjct: 61  RLCN---KSIKCVFSSHSDGNGSTAENFNENDEEYVNSSVVEAVEVKSGADGFMVKMRDG 120

Query: 153 RHLRCVHNNPHGGHLPDYAPHPAIVLKMEDGTGLLLPIIVLEMPSVLLMAAVRNVPIARP 212
           R LRCVHNNP GGHLPDYAPHPAIVLKMEDGTGLLLPIIVLEMPSVLLMAA+ NV IARP
Sbjct: 121 RQLRCVHNNPQGGHLPDYAPHPAIVLKMEDGTGLLLPIIVLEMPSVLLMAAMTNVQIARP 180

Query: 213 TMYQVVKEMIDKMGYE----------------------IGCDTDCLSFDLRPSDAINIAV 272
           TMYQVVKEM+DKMGYE                      +G  ++C+SFDLRPSDAINIAV
Sbjct: 181 TMYQVVKEMVDKMGYEVRLVRVTKRVHEAYFAQLFLSKVGNASECVSFDLRPSDAINIAV 240

Query: 273 RCKVPIQVNKYLAYSDGMRVVESGKLSTQAPATDGLLFTELDRPSGQPCVEAKEFNLVRN 328
           RCK+PIQVNKYLAYSDGMRV+ESGK+ST APA+DGLLFTE DRP+GQ C++ KEFN++  
Sbjct: 241 RCKIPIQVNKYLAYSDGMRVIESGKISTPAPASDGLLFTEQDRPNGQACLDTKEFNILSK 300

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
XP_008466529.13.2e-15789.91PREDICTED: bifunctional nuclease 1 [Cucumis melo] >XP_008466530.1 PREDICTED: bif... [more]
XP_038898951.11.8e-15588.96bifunctional nuclease 1 [Benincasa hispida] >XP_038898953.1 bifunctional nucleas... [more]
KAG7037875.11.1e-15492.88Bifunctional nuclease 2 [Cucurbita argyrosperma subsp. argyrosperma][more]
XP_023525215.13.1e-15287.07bifunctional nuclease 1-like [Cucurbita pepo subsp. pepo] >XP_023525216.1 bifunc... [more]
XP_004147851.15.3e-15287.70bifunctional nuclease 1 [Cucumis sativus] >XP_011652430.1 bifunctional nuclease ... [more]
Match NameE-valueIdentityDescription
Q93VH21.6e-10662.78Bifunctional nuclease 2 OS=Arabidopsis thaliana OX=3702 GN=BBD2 PE=2 SV=1[more]
Q9FWS63.5e-10663.75Bifunctional nuclease 1 OS=Arabidopsis thaliana OX=3702 GN=BBD1 PE=2 SV=1[more]
Q6YZM62.0e-8564.57Bifunctional nuclease 2 OS=Oryza sativa subsp. japonica OX=39947 GN=BBD2 PE=2 SV... [more]
Q5N8J36.5e-6853.33Bifunctional nuclease 1 OS=Oryza sativa subsp. japonica OX=39947 GN=BBD1 PE=2 SV... [more]
Q09LL36.5e-6853.33Bifunctional nuclease OS=Oryza minuta OX=63629 GN=BBD PE=2 SV=1[more]
Match NameE-valueIdentityDescription
A0A1S3CRI01.6e-15789.91bifunctional nuclease 1 OS=Cucumis melo OX=3656 GN=LOC103503917 PE=3 SV=1[more]
A0A5D3D8741.6e-15789.91Bifunctional nuclease 1 OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold... [more]
A0A0A0LDD42.6e-15287.70BFN domain-containing protein OS=Cucumis sativus OX=3659 GN=Csa_3G860260 PE=3 SV... [more]
A0A6J1FM733.7e-15186.44bifunctional nuclease 1-like OS=Cucurbita moschata OX=3662 GN=LOC111446486 PE=3 ... [more]
A0A6J1IXM93.1e-15085.49bifunctional nuclease 1-like OS=Cucurbita maxima OX=3661 GN=LOC111480857 PE=3 SV... [more]
Match NameE-valueIdentityDescription
AT1G19660.11.1e-10762.78Wound-responsive family protein [more]
AT1G19660.21.1e-10762.78Wound-responsive family protein [more]
AT1G75380.12.5e-10763.75bifunctional nuclease in basal defense response 1 [more]
AT1G75380.22.5e-10763.75bifunctional nuclease in basal defense response 1 [more]
AT1G75380.32.5e-10763.75bifunctional nuclease in basal defense response 1 [more]
InterPro
Analysis Name: InterPro Annotations of Watermelon (97103) v2.5
Date Performed: 2022-01-31
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR036104Bifunctional nuclease superfamilyGENE3D3.10.690.10Bifunctional nuclease domaincoord: 161..227
e-value: 3.1E-7
score: 31.8
coord: 228..273
e-value: 5.2E-11
score: 44.1
IPR036104Bifunctional nuclease superfamilySUPERFAMILY103256Hypothetical protein TM0160coord: 154..271
IPR003729Bifunctional nuclease domainPFAMPF02577DUF151coord: 233..262
e-value: 2.4E-5
score: 24.0
NoneNo IPR availablePANTHERPTHR15160:SF16NUCLEASE IN BASAL DEFENSE RESPONSE PROTEINcoord: 33..331
NoneNo IPR availablePANTHERPTHR15160VON HIPPEL-LINDAU PROTEINcoord: 33..331

Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
Cla97C05G100120.2Cla97C05G100120.2mRNA


GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
biological_process GO:0090305 nucleic acid phosphodiester bond hydrolysis
biological_process GO:0016567 protein ubiquitination
cellular_component GO:0016021 integral component of membrane
cellular_component GO:0005634 nucleus
cellular_component GO:0030891 VCB complex
molecular_function GO:0004518 nuclease activity