Cla97C05G099750 (gene) Watermelon (97103) v2.5

Overview


Name	Cla97C05G099750
Type	gene
Organism	Citrullus lanatus subsp. vulgaris cv. 97103 (Watermelon (97103) v2.5)
Description	iron-sulfur assembly protein IscA-like 1, mitochondrial
Location	Cla97Chr05: 28735012 .. 28737943 (-)
RNA-Seq Expression	Cla97C05G099750
Synteny	Cla97C05G099750

Sequences

The following sequences are available for this feature:

Gene sequence (with intron)

Legend: exonfive_prime_UTRpolypeptideCDSthree_prime_UTR

Hold the cursor over a type above to highlight its positions in the sequence below.

AAATGAGATATAACGGAAATCACACGGAAAGAAAATGTGAAACTTGTAATTGGAAATTTGGAGAGTAAATGAAATCTTCGTGAGTGTCTCCGCAGAGGAGAAAATTGATTGATAGGCAGCAAGAGGAGAGAAAATGGCAGCTTCTTCGTCTTCCTTTTTCGCCGCAGCTTTTCAGAAGGTCGGACCAGCCGCTCGCCGCCAAATTCTTACTCTGACGGATGCCGCCGCTGCTAGAATTCGACAACTTCTTCAACAGCGCCAACGTCCATTCCTCCGCCTTGGCGTCAAGGCGCGCGGTTGTAATGGCTTATCCTATACTCTCAACTACGCAGGTTCGTAATAACCTAATCGATTTCAACTTTTTTATTGATTCTACGCGATTTCTTTTTTACTCATTTTATGTCTGAATTTAAGGATGGGATGAAATTTGTCGATTGTCTACTCTTTTTTCTTTGAATTAGATGTTGCTGTTTGGATTTGATTCGAAATAAATAGGTATTTGGTGTATTTGAGCGATATAACCTAATAGAGATGTTTCGAAACTGGAGTCTTTTAAAAGATGAATTATTTATCTTTGAACTGTTTCCTGCTCCAATTTCCAAAAATTAGGATTTTAAGACCGAGGATCTATTGTGATGATTAAACGCGGAAAACTAGAAGGTTAAATGATTCTGTAATTGTTATAACCGGAATTCGGCCTGGGTATGTTATGTAAGTAGTTCGAAGATGATCTGTTGAGTTCTTGCTAATGTAACCTTAACAACGGCAAATCTGGAAGATAAAACTGAAATGCCTGAATGCTTAAATCGATAGAAAAGAGATGATTTTACATGCGGGGACCTATCTATGAGAGGAAAATTGACTTAACTAAACTACTTCAGTCTGTAAATCATTCTGGATCTTACAGAATCAGTTATGAATTAATGGTGTCATTCGACGATTTACTTAACAGACTTATGACATAGGGATTAGTGTTGTTATTGATATATCACAATGCCAAACTAAATGCATGGTCCACCCAAATTTATGATGAACATTTGCTTCAATCAAGTACCATTTAATTTGGGGTGTATTTTGGCTATTGTGAGAACATAGCAGGATTGGACATCGATTAAATTATACTTGAGAACCTTTGTCTCCCTCTCTCTCTCTCTCTCTCTCCTGATATGGTTAATTAGAATGTTATCGGACCAAAGTGGTGTTTCTATTCAATTAGAAGATAAATAGGTTAGGAAGCCTTACATGGGTCGACTTGGAGAGACTAGTTATGAACATGTTAGATGGTCAGAAATTTGAGATTTTATACACTTTGTGGTTTGGGATTTTTTATTGCCTGTGTTTATTTAATATGGCTAGCTTCTCAGTTAACAGTACTTACAATTCAGTTTCTTATGGAGTTGCTCATGATTGGAAAATTTTCTAACTCAGCATTACCTCAGTTTGATAATCACATTATCATTAACAATTTCTTTGTGCAAGAAATAGGATTCAACCTTCATCTCTCTTTTGGCATAAATTGACTATAGTTATGATCTCTATATTCCGGAATACTTCAAGATTGCAGTATTTCATTTAACGTGTCCTTTTTCAAATATTTCAGATGAGAAAGGGAAGTTTGATGAGCTGGTTGAGGATCAAAATGTGAAGATACTGATTGATCCTAAGGCTCTGATGCACGTAATAGGAACAAAGATGGACTTTGTCGATGACAAATTGAGGTATTTGAGAAATCTCTTTCCTACAATATTATGTTTTCAGCTCTATTTCCATCCATATGTCGATGAAACATCCTGCTTGTTGGTTACAAAATCAGGCCGACATATAAAAGCTTGTGTATATTTAAATGTCAACAGTAAGCGTCTGCATCTTATATAAAAAGTTGTAAACCTATTGTTTCACACTTAAACATATTTTAGGAGGTTTTAATCGGCCGTAAACCATCCCCATCCTGTATTTGCTTGTAAATCAATTTTCTACAGTGATGGAGGTATTTGCAACATGCAAGGACCAGATAATTCATTTGCATGTATATGATAGGGAGCATCTCTGCTTGAGAATGGGTTCAGATCATCTTTAGCATCCATTAGCTGGTGAATATAATTATTACAACTTAAGAGAGAACTGTTATGTTTATTAGAGTCACTCAATTTGAGCAAATTTTAGCTCAAAATTTTAGTAGATAATGTACTAATTGCCTTTGTGATCCTCTACCTTGCAATTGTTGAACAAAAAAAATAAGTTCATTAGATTCAGTTTATCTTGAGTCTCGAGACTTTTGAGTACTCGAGACTCTTGATGCTCAAAGAAATTCAAATTCAACTTTTTGTGAACCCCCTGTACTGCTATATTCAGTGGCCAATGAAGCTTTTTTTTTTTTCCTCTCTCTCTTCTTGGTAGATCTGAGTTTATCTTTATCAATCCAAACTCCAAAGGTCAATGTGGCTGTGGGGAGTCCTTTATGACAACAGGCAGTGCAGAAGCCAACAAAAGGTGAGGCAGTTCGAGTAATGAGCACTGTTTTAAAAACTCAAAACTCAGTTGCTGATTTGTTGTGTATTTTGCTGGAATTGCAAGGACAATAACCAAGAGAGTCCTCTGACAAGAACTTAAAAACAGTTGTAAGAATATTTCTATATTGGCTAATTATATTCTTAGAAAACCAGTTAACCGTTGTATACTTTTTTTTGGTATAATATATTAGTAGATTGAACTGGCTAAATGAGCTATGAGGTAATAAAATTCTCCCACGACTTTCTTTTATTATAGGACTATCCTGCGTGCCTAATAACTAGTTGAGTGATATGCATGCCTAATAACTAATTGAGTGATAAGAATGGTTTGTGTCCGCCAAAATGAGCATAGCTCAACAGAAACTAACATGAACTCATTTCTTTGAAATTGAGGTTCAATTCTTCATTCTACCACTTGTTGC

mRNA sequence

AAATGAGATATAACGGAAATCACACGGAAAGAAAATGTGAAACTTGTAATTGGAAATTTGGAGAGTAAATGAAATCTTCGTGAGTGTCTCCGCAGAGGAGAAAATTGATTGATAGGCAGCAAGAGGAGAGAAAATGGCAGCTTCTTCGTCTTCCTTTTTCGCCGCAGCTTTTCAGAAGGTCGGACCAGCCGCTCGCCGCCAAATTCTTACTCTGACGGATGCCGCCGCTGCTAGAATTCGACAACTTCTTCAACAGCGCCAACGTCCATTCCTCCGCCTTGGCGTCAAGGCGCGCGGTTGTAATGGCTTATCCTATACTCTCAACTACGCAGCTTCTCAGTTAACAGTACTTACAATTCAGTTTCTTATGGAGTTGCTCATGATTGGAAAATTTTCTAACTCAGCATTACCTCATTATGATCTCTATATTCCGGAATACTTCAAGATTGCAGTATTTCATTTAACGTGTCCTTTTTCAAATATTTCAGATGAGAAAGGGAAGTTTGATGAGCTGGTTGAGGATCAAAATGTGAAGATACTGATTGATCCTAAGGCTCTGATGCACGTAATAGGAACAAAGATGGACTTTGTCGATGACAAATTGAGATCTGAGTTTATCTTTATCAATCCAAACTCCAAAGGTCAATGTGGCTGTGGGGAGTCCTTTATGACAACAGGCAGTGCAGAAGCCAACAAAAGGTGAGGCAGTTCGAGTAATGAGCACTGTTTTAAAAACTCAAAACTCAGTTGCTGATTTGTTGTGTATTTTGCTGGAATTGCAAGGACAATAACCAAGAGAGTCCTCTGACAAGAACTTAAAAACAGTTGTAAGAATATTTCTATATTGGCTAATTATATTCTTAGAAAACCAGTTAACCGTTGTATACTTTTTTTTGGTATAATATATTAGTAGATTGAACTGGCTAAATGAGCTATGAGGTAATAAAATTCTCCCACGACTTTCTTTTATTATAGGACTATCCTGCGTGCCTAATAACTAGTTGAGTGATATGCATGCCTAATAACTAATTGAGTGATAAGAATGGTTTGTGTCCGCCAAAATGAGCATAGCTCAACAGAAACTAACATGAACTCATTTCTTTGAAATTGAGGTTCAATTCTTCATTCTACCACTTGTTGC

Coding sequence (CDS)

ATGGCAGCTTCTTCGTCTTCCTTTTTCGCCGCAGCTTTTCAGAAGGTCGGACCAGCCGCTCGCCGCCAAATTCTTACTCTGACGGATGCCGCCGCTGCTAGAATTCGACAACTTCTTCAACAGCGCCAACGTCCATTCCTCCGCCTTGGCGTCAAGGCGCGCGGTTGTAATGGCTTATCCTATACTCTCAACTACGCAGCTTCTCAGTTAACAGTACTTACAATTCAGTTTCTTATGGAGTTGCTCATGATTGGAAAATTTTCTAACTCAGCATTACCTCATTATGATCTCTATATTCCGGAATACTTCAAGATTGCAGTATTTCATTTAACGTGTCCTTTTTCAAATATTTCAGATGAGAAAGGGAAGTTTGATGAGCTGGTTGAGGATCAAAATGTGAAGATACTGATTGATCCTAAGGCTCTGATGCACGTAATAGGAACAAAGATGGACTTTGTCGATGACAAATTGAGATCTGAGTTTATCTTTATCAATCCAAACTCCAAAGGTCAATGTGGCTGTGGGGAGTCCTTTATGACAACAGGCAGTGCAGAAGCCAACAAAAGGTGA

Protein sequence

MAASSSSFFAAAFQKVGPAARRQILTLTDAAAARIRQLLQQRQRPFLRLGVKARGCNGLSYTLNYAASQLTVLTIQFLMELLMIGKFSNSALPHYDLYIPEYFKIAVFHLTCPFSNISDEKGKFDELVEDQNVKILIDPKALMHVIGTKMDFVDDKLRSEFIFINPNSKGQCGCGESFMTTGSAEANKR

Homology

[Close]

BLAST of Cla97C05G099750 vs. NCBI nr
Match: XP_038891787.1 (iron-sulfur assembly protein IscA-like 1, mitochondrial isoform X1 [Benincasa hispida])

HSP 1 Score: 237.7 bits (605), Expect = 8.5e-59
Identity = 134/189 (70.90%), Postives = 136/189 (71.96%), Query Frame = 0


Query: 1   MAASSSSFFAAAFQKVGPAARRQILTLTDAAAARIRQLLQQRQRPFLRLGVKARGCNGLS 60
           MAASSSSFFAAA QKVGPAARRQILTLT+AAAARIRQLLQQRQRPFLRLGVKARGCNGLS
Sbjct: 1   MAASSSSFFAAASQKVGPAARRQILTLTEAAAARIRQLLQQRQRPFLRLGVKARGCNGLS 60

Query: 61  YTLNYAASQLTVLTIQFLMELLMIGKFSNSALPHYDLYIPEYFKIAVFHLTCPFSNISDE 120
           YTLNYA                                                    DE
Sbjct: 61  YTLNYA----------------------------------------------------DE 120

Query: 121 KGKFDELVEDQNVKILIDPKALMHVIGTKMDFVDDKLRSEFIFINPNSKGQCGCGESFMT 180
           KGKFDELVEDQNVKILIDPKALMHVIGTKMDFVDDKLRSEFIFINPNSKGQCGCGESFMT
Sbjct: 121 KGKFDELVEDQNVKILIDPKALMHVIGTKMDFVDDKLRSEFIFINPNSKGQCGCGESFMT 137

Query: 181 TGSAEANKR 190
           TGSAEANK+
Sbjct: 181 TGSAEANKK 137

[Close]

BLAST of Cla97C05G099750 vs. NCBI nr
Match: XP_022987453.1 (iron-sulfur assembly protein IscA-like 1, mitochondrial [Cucurbita maxima])

HSP 1 Score: 236.1 bits (601), Expect = 2.5e-58
Identity = 133/189 (70.37%), Postives = 136/189 (71.96%), Query Frame = 0


Query: 1   MAASSSSFFAAAFQKVGPAARRQILTLTDAAAARIRQLLQQRQRPFLRLGVKARGCNGLS 60
           MAASSSSFFAAA QKVGPAARRQILTLT+AAAARIRQLLQQRQRPFLRLGVKARGCNGLS
Sbjct: 19  MAASSSSFFAAASQKVGPAARRQILTLTEAAAARIRQLLQQRQRPFLRLGVKARGCNGLS 78

Query: 61  YTLNYAASQLTVLTIQFLMELLMIGKFSNSALPHYDLYIPEYFKIAVFHLTCPFSNISDE 120
           YTLNYA                                                    DE
Sbjct: 79  YTLNYA----------------------------------------------------DE 138

Query: 121 KGKFDELVEDQNVKILIDPKALMHVIGTKMDFVDDKLRSEFIFINPNSKGQCGCGESFMT 180
           KGKFDELVEDQNVKILI+PKALMHVIGTKMDFVDDKLRSEFIFINPNSKGQCGCGESFMT
Sbjct: 139 KGKFDELVEDQNVKILIEPKALMHVIGTKMDFVDDKLRSEFIFINPNSKGQCGCGESFMT 155

Query: 181 TGSAEANKR 190
           TGSAEANK+
Sbjct: 199 TGSAEANKK 155

[Close]

BLAST of Cla97C05G099750 vs. NCBI nr
Match: KAG7032017.1 (Iron-sulfur assembly protein IscA-like 1, mitochondrial [Cucurbita argyrosperma subsp. argyrosperma])

HSP 1 Score: 235.7 bits (600), Expect = 3.2e-58
Identity = 132/189 (69.84%), Postives = 136/189 (71.96%), Query Frame = 0


Query: 1   MAASSSSFFAAAFQKVGPAARRQILTLTDAAAARIRQLLQQRQRPFLRLGVKARGCNGLS 60
           MAASSSSFFAAA QKVGPAARRQILTLT+AAAARIRQLLQQRQRPFLRLGVKARGCNGLS
Sbjct: 19  MAASSSSFFAAASQKVGPAARRQILTLTEAAAARIRQLLQQRQRPFLRLGVKARGCNGLS 78

Query: 61  YTLNYAASQLTVLTIQFLMELLMIGKFSNSALPHYDLYIPEYFKIAVFHLTCPFSNISDE 120
           YTLNYA                                                    DE
Sbjct: 79  YTLNYA----------------------------------------------------DE 138

Query: 121 KGKFDELVEDQNVKILIDPKALMHVIGTKMDFVDDKLRSEFIFINPNSKGQCGCGESFMT 180
           KGKFDELVEDQNVKILI+PKALMHVIGTKMDF+DDKLRSEFIFINPNSKGQCGCGESFMT
Sbjct: 139 KGKFDELVEDQNVKILIEPKALMHVIGTKMDFIDDKLRSEFIFINPNSKGQCGCGESFMT 155

Query: 181 TGSAEANKR 190
           TGSAEANK+
Sbjct: 199 TGSAEANKK 155

[Close]

BLAST of Cla97C05G099750 vs. NCBI nr
Match: XP_022956980.1 (iron-sulfur assembly protein IscA-like 1, mitochondrial [Cucurbita moschata])

HSP 1 Score: 232.3 bits (591), Expect = 3.6e-57
Identity = 131/189 (69.31%), Postives = 135/189 (71.43%), Query Frame = 0


Query: 1   MAASSSSFFAAAFQKVGPAARRQILTLTDAAAARIRQLLQQRQRPFLRLGVKARGCNGLS 60
           MAASSSSF AAA QKVGPAARR+ILTLT+AAAARIRQLLQQRQRPFLRLGVKARGCNGLS
Sbjct: 1   MAASSSSFLAAASQKVGPAARRKILTLTEAAAARIRQLLQQRQRPFLRLGVKARGCNGLS 60

Query: 61  YTLNYAASQLTVLTIQFLMELLMIGKFSNSALPHYDLYIPEYFKIAVFHLTCPFSNISDE 120
           YTLNYA                                                    DE
Sbjct: 61  YTLNYA----------------------------------------------------DE 120

Query: 121 KGKFDELVEDQNVKILIDPKALMHVIGTKMDFVDDKLRSEFIFINPNSKGQCGCGESFMT 180
           KGKFDELVEDQNVKILI+PKALMHVIGTKMDFVDDKLRSEFIFINPNSKGQCGCGESFMT
Sbjct: 121 KGKFDELVEDQNVKILIEPKALMHVIGTKMDFVDDKLRSEFIFINPNSKGQCGCGESFMT 137

Query: 181 TGSAEANKR 190
           TGSAEANK+
Sbjct: 181 TGSAEANKK 137

[Close]

BLAST of Cla97C05G099750 vs. NCBI nr
Match: XP_004135035.1 (iron-sulfur assembly protein IscA-like 1, mitochondrial [Cucumis sativus] >KGN52189.1 hypothetical protein Csa_009361 [Cucumis sativus])

HSP 1 Score: 232.3 bits (591), Expect = 3.6e-57
Identity = 131/189 (69.31%), Postives = 134/189 (70.90%), Query Frame = 0


Query: 1   MAASSSSFFAAAFQKVGPAARRQILTLTDAAAARIRQLLQQRQRPFLRLGVKARGCNGLS 60
           MAASSSSFFAAA QK GPAARRQILTLT+AAAARIRQLLQQRQRPFLRLGVKARGCNGLS
Sbjct: 1   MAASSSSFFAAASQKAGPAARRQILTLTEAAAARIRQLLQQRQRPFLRLGVKARGCNGLS 60

Query: 61  YTLNYAASQLTVLTIQFLMELLMIGKFSNSALPHYDLYIPEYFKIAVFHLTCPFSNISDE 120
           YTLNYA                                                    DE
Sbjct: 61  YTLNYA----------------------------------------------------DE 120

Query: 121 KGKFDELVEDQNVKILIDPKALMHVIGTKMDFVDDKLRSEFIFINPNSKGQCGCGESFMT 180
           KGKFDELVEDQNVKILI+PKALMHVIGTKMDFVDDKLRSEFIFINPNSKGQCGCGESFMT
Sbjct: 121 KGKFDELVEDQNVKILIEPKALMHVIGTKMDFVDDKLRSEFIFINPNSKGQCGCGESFMT 137

Query: 181 TGSAEANKR 190
           T SAEANK+
Sbjct: 181 TSSAEANKK 137

[Close]

BLAST of Cla97C05G099750 vs. ExPASy Swiss-Prot
Match: Q8LBM4 (Iron-sulfur assembly protein IscA-like 1, mitochondrial OS=Arabidopsis thaliana OX=3702 GN=At2g16710 PE=2 SV=2)

HSP 1 Score: 189.5 bits (480), Expect = 3.5e-47
Identity = 102/184 (55.43%), Postives = 119/184 (64.67%), Query Frame = 0


Query: 5   SSSFFAAAFQKVGPAARRQILTLTDAAAARIRQLLQQRQRPFLRLGVKARGCNGLSYTLN 64
           +S   AAA  +VGPA R+Q+LTLTD AA+R+  LLQQRQ+PFLRLGVKARGCNGLSYTLN
Sbjct: 3   ASQILAAAAARVGPALRKQVLTLTDEAASRVHHLLQQRQKPFLRLGVKARGCNGLSYTLN 62

Query: 65  YAASQLTVLTIQFLMELLMIGKFSNSALPHYDLYIPEYFKIAVFHLTCPFSNISDEKGKF 124
           YA                                                    DEKGKF
Sbjct: 63  YA----------------------------------------------------DEKGKF 122

Query: 125 DELVEDQNVKILIDPKALMHVIGTKMDFVDDKLRSEFIFINPNSKGQCGCGESFMTTGSA 184
           DELVE++ V+IL++PKALMHVIGTKMDFVDDKLRSEF+FINPNS+GQCGCGESFMTT ++
Sbjct: 123 DELVEEKGVRILVEPKALMHVIGTKMDFVDDKLRSEFVFINPNSQGQCGCGESFMTTSTS 134

Query: 185 EANK 189
            A +
Sbjct: 183 SAKQ 134

[Close]

BLAST of Cla97C05G099750 vs. ExPASy Swiss-Prot
Match: Q8L8C0 (Iron-sulfur assembly protein IscA-like 3, mitochondrial OS=Arabidopsis thaliana OX=3702 GN=At2g36260 PE=3 SV=2)

HSP 1 Score: 151.4 bits (381), Expect = 1.0e-35
Identity = 82/161 (50.93%), Postives = 99/161 (61.49%), Query Frame = 0


Query: 21  RRQILTLTDAAAARIRQLLQQRQRPFLRLGVKARGCNGLSYTLNYAASQLTVLTIQFLME 80
           R+Q+L L+D AAARIRQLLQ +Q+PFLRL V+A+GCNGLSY LNYA              
Sbjct: 2   RKQVLALSDTAAARIRQLLQHQQKPFLRLAVEAKGCNGLSYVLNYA-------------- 61

Query: 81  LLMIGKFSNSALPHYDLYIPEYFKIAVFHLTCPFSNISDEKGKFDELVEDQNVKILIDPK 140
                                                  EKGKFDE+VE++ VKIL+DPK
Sbjct: 62  --------------------------------------QEKGKFDEVVEEKGVKILVDPK 109

Query: 141 ALMHVIGTKMDFVDDKLRSEFIFINPNSKGQCGCGESFMTT 182
           A+MHVIGT+MDFVDDKLRSEF+F+NPN+  +CGCGESF TT
Sbjct: 122 AVMHVIGTEMDFVDDKLRSEFVFVNPNAT-KCGCGESFTTT 109

[Close]

BLAST of Cla97C05G099750 vs. ExPASy Swiss-Prot
Match: Q07821 (Iron-sulfur assembly protein 1 OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=ISA1 PE=1 SV=1)

HSP 1 Score: 102.1 bits (253), Expect = 7.3e-21
Identity = 58/159 (36.48%), Postives = 78/159 (49.06%), Query Frame = 0


Query: 21  RRQILTLTDAAAARIRQLLQQRQRPFLRLGVKARGCNGLSYTLNYAASQLTVLTIQFLME 80
           R+ ++TL+  A   +R LL Q +   +R+  + RGC+GL+Y L Y               
Sbjct: 143 RKALITLSPKAIKHLRALLAQPEPKLIRVSARNRGCSGLTYDLQYIT------------- 202

Query: 81  LLMIGKFSNSALPHYDLYIPEYFKIAVFHLTCPFSNISDEKGKFDELVEDQNVKILIDPK 140
                                                  E GKFDE+VE   VKI+ID K
Sbjct: 203 ---------------------------------------EPGKFDEVVEQDGVKIVIDSK 249

Query: 141 ALMHVIGTKMDFVDDKLRSEFIFINPNSKGQCGCGESFM 180
           AL  +IG++MD++DDKL S+F+F NPNSKG CGCGESFM
Sbjct: 263 ALFSIIGSEMDWIDDKLASKFVFKNPNSKGTCGCGESFM 249

[Close]

BLAST of Cla97C05G099750 vs. ExPASy Swiss-Prot
Match: Q54VS1 (Iron-sulfur cluster assembly 1 homolog, mitochondrial OS=Dictyostelium discoideum OX=44689 GN=isca1 PE=3 SV=1)

HSP 1 Score: 99.4 bits (246), Expect = 4.7e-20
Identity = 55/170 (32.35%), Postives = 90/170 (52.94%), Query Frame = 0


Query: 15  KVGPAARRQILTLTDAAAARIRQLLQQRQRP---FLRLGVKARGCNGLSYTLNYAASQLT 74
           K+G  +++ I ++TD+A  R+++++  ++      LRLG+K RGC+G+SYTL++A     
Sbjct: 8   KLGKPSKKAIFSMTDSALKRVKEIMNDKKIDNCIGLRLGIKERGCSGMSYTLDFAT---- 67

Query: 75  VLTIQFLMELLMIGKFSNSALPHYDLYIPEYFKIAVFHLTCPFSNISDEKGKFDE-LVED 134
                                                           +K KFDE +V D
Sbjct: 68  ------------------------------------------------QKNKFDETVVAD 125

Query: 135 QNVKILIDPKALMHVIGTKMDFVDDKLRSEFIFINPNSKGQCGCGESFMT 181
           +++ I++D KAL+ VIGT+MD++++ ++ EFIFINPN+   CGCGESF T
Sbjct: 128 KDINIIVDSKALLSVIGTEMDYIEEPIKKEFIFINPNATNTCGCGESFTT 125

[Close]

BLAST of Cla97C05G099750 vs. ExPASy Swiss-Prot
Match: Q8T3X9 (Iron-sulfur cluster assembly 1 homolog, mitochondrial OS=Drosophila melanogaster OX=7227 GN=MagR PE=1 SV=1)

HSP 1 Score: 99.0 bits (245), Expect = 6.2e-20
Identity = 62/158 (39.24%), Postives = 77/158 (48.73%), Query Frame = 0


Query: 22  RQILTLTDAAAARIRQLLQQR-QRPFLRLGVKARGCNGLSYTLNYAASQLTVLTIQFLME 81
           R  LTLT AA  RI+ LLQ +     L++GV+ RGCNGLSYTL+YA+             
Sbjct: 23  RAALTLTPAAVLRIKTLLQDKPDMVGLKVGVRQRGCNGLSYTLDYAS------------- 82

Query: 82  LLMIGKFSNSALPHYDLYIPEYFKIAVFHLTCPFSNISDEKGKFDELVEDQNVKILIDPK 141
                                                  +K K DE V    VK+ ID K
Sbjct: 83  ---------------------------------------QKDKLDEEVVQDGVKVFIDKK 128

Query: 142 ALMHVIGTKMDFVDDKLRSEFIFINPNSKGQCGCGESF 179
           A + ++GT+MDFV+ KL SEF+F NPN KG CGCGESF
Sbjct: 143 AQLSLLGTEMDFVESKLSSEFVFNNPNIKGTCGCGESF 128

[Close]

BLAST of Cla97C05G099750 vs. ExPASy TrEMBL
Match: A0A6J1JJH4 (iron-sulfur assembly protein IscA-like 1, mitochondrial OS=Cucurbita maxima OX=3661 GN=LOC111484969 PE=4 SV=1)

HSP 1 Score: 236.1 bits (601), Expect = 1.2e-58
Identity = 133/189 (70.37%), Postives = 136/189 (71.96%), Query Frame = 0


Query: 1   MAASSSSFFAAAFQKVGPAARRQILTLTDAAAARIRQLLQQRQRPFLRLGVKARGCNGLS 60
           MAASSSSFFAAA QKVGPAARRQILTLT+AAAARIRQLLQQRQRPFLRLGVKARGCNGLS
Sbjct: 19  MAASSSSFFAAASQKVGPAARRQILTLTEAAAARIRQLLQQRQRPFLRLGVKARGCNGLS 78

Query: 61  YTLNYAASQLTVLTIQFLMELLMIGKFSNSALPHYDLYIPEYFKIAVFHLTCPFSNISDE 120
           YTLNYA                                                    DE
Sbjct: 79  YTLNYA----------------------------------------------------DE 138

Query: 121 KGKFDELVEDQNVKILIDPKALMHVIGTKMDFVDDKLRSEFIFINPNSKGQCGCGESFMT 180
           KGKFDELVEDQNVKILI+PKALMHVIGTKMDFVDDKLRSEFIFINPNSKGQCGCGESFMT
Sbjct: 139 KGKFDELVEDQNVKILIEPKALMHVIGTKMDFVDDKLRSEFIFINPNSKGQCGCGESFMT 155

Query: 181 TGSAEANKR 190
           TGSAEANK+
Sbjct: 199 TGSAEANKK 155

[Close]

BLAST of Cla97C05G099750 vs. ExPASy TrEMBL
Match: A0A5A7SVZ7 (Iron-sulfur assembly protein IscA-like 1 OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold475G001970 PE=4 SV=1)

HSP 1 Score: 232.3 bits (591), Expect = 1.7e-57
Identity = 131/189 (69.31%), Postives = 133/189 (70.37%), Query Frame = 0


Query: 1   MAASSSSFFAAAFQKVGPAARRQILTLTDAAAARIRQLLQQRQRPFLRLGVKARGCNGLS 60
           MAASSSSFFAAA QK GPA RRQILTLT+AAAARIRQLLQQRQRPFLRLGVKARGCNGLS
Sbjct: 1   MAASSSSFFAAASQKAGPAVRRQILTLTEAAAARIRQLLQQRQRPFLRLGVKARGCNGLS 60

Query: 61  YTLNYAASQLTVLTIQFLMELLMIGKFSNSALPHYDLYIPEYFKIAVFHLTCPFSNISDE 120
           YTLNYA                                                    DE
Sbjct: 61  YTLNYA----------------------------------------------------DE 120

Query: 121 KGKFDELVEDQNVKILIDPKALMHVIGTKMDFVDDKLRSEFIFINPNSKGQCGCGESFMT 180
           KGKFDELVEDQNVKILIDPKALMHVIGTKMDFVDDKLRSEFIFINPNSKGQCGCGESFMT
Sbjct: 121 KGKFDELVEDQNVKILIDPKALMHVIGTKMDFVDDKLRSEFIFINPNSKGQCGCGESFMT 137

Query: 181 TGSAEANKR 190
           T SAEANK+
Sbjct: 181 TSSAEANKK 137

[Close]

BLAST of Cla97C05G099750 vs. ExPASy TrEMBL
Match: A0A0A0KTF0 (Fe-S_biosyn domain-containing protein OS=Cucumis sativus OX=3659 GN=Csa_5G614650 PE=4 SV=1)

HSP 1 Score: 232.3 bits (591), Expect = 1.7e-57
Identity = 131/189 (69.31%), Postives = 134/189 (70.90%), Query Frame = 0


Query: 1   MAASSSSFFAAAFQKVGPAARRQILTLTDAAAARIRQLLQQRQRPFLRLGVKARGCNGLS 60
           MAASSSSFFAAA QK GPAARRQILTLT+AAAARIRQLLQQRQRPFLRLGVKARGCNGLS
Sbjct: 1   MAASSSSFFAAASQKAGPAARRQILTLTEAAAARIRQLLQQRQRPFLRLGVKARGCNGLS 60

Query: 61  YTLNYAASQLTVLTIQFLMELLMIGKFSNSALPHYDLYIPEYFKIAVFHLTCPFSNISDE 120
           YTLNYA                                                    DE
Sbjct: 61  YTLNYA----------------------------------------------------DE 120

Query: 121 KGKFDELVEDQNVKILIDPKALMHVIGTKMDFVDDKLRSEFIFINPNSKGQCGCGESFMT 180
           KGKFDELVEDQNVKILI+PKALMHVIGTKMDFVDDKLRSEFIFINPNSKGQCGCGESFMT
Sbjct: 121 KGKFDELVEDQNVKILIEPKALMHVIGTKMDFVDDKLRSEFIFINPNSKGQCGCGESFMT 137

Query: 181 TGSAEANKR 190
           T SAEANK+
Sbjct: 181 TSSAEANKK 137

[Close]

BLAST of Cla97C05G099750 vs. ExPASy TrEMBL
Match: A0A6J1GZ87 (iron-sulfur assembly protein IscA-like 1, mitochondrial OS=Cucurbita moschata OX=3662 GN=LOC111458498 PE=4 SV=1)

HSP 1 Score: 232.3 bits (591), Expect = 1.7e-57
Identity = 131/189 (69.31%), Postives = 135/189 (71.43%), Query Frame = 0


Query: 1   MAASSSSFFAAAFQKVGPAARRQILTLTDAAAARIRQLLQQRQRPFLRLGVKARGCNGLS 60
           MAASSSSF AAA QKVGPAARR+ILTLT+AAAARIRQLLQQRQRPFLRLGVKARGCNGLS
Sbjct: 1   MAASSSSFLAAASQKVGPAARRKILTLTEAAAARIRQLLQQRQRPFLRLGVKARGCNGLS 60

Query: 61  YTLNYAASQLTVLTIQFLMELLMIGKFSNSALPHYDLYIPEYFKIAVFHLTCPFSNISDE 120
           YTLNYA                                                    DE
Sbjct: 61  YTLNYA----------------------------------------------------DE 120

Query: 121 KGKFDELVEDQNVKILIDPKALMHVIGTKMDFVDDKLRSEFIFINPNSKGQCGCGESFMT 180
           KGKFDELVEDQNVKILI+PKALMHVIGTKMDFVDDKLRSEFIFINPNSKGQCGCGESFMT
Sbjct: 121 KGKFDELVEDQNVKILIEPKALMHVIGTKMDFVDDKLRSEFIFINPNSKGQCGCGESFMT 137

Query: 181 TGSAEANKR 190
           TGSAEANK+
Sbjct: 181 TGSAEANKK 137

[Close]

BLAST of Cla97C05G099750 vs. ExPASy TrEMBL
Match: A0A1S3BFV2 (iron-sulfur assembly protein IscA-like 1, mitochondrial OS=Cucumis melo OX=3656 GN=LOC103489389 PE=4 SV=1)

HSP 1 Score: 232.3 bits (591), Expect = 1.7e-57
Identity = 131/189 (69.31%), Postives = 133/189 (70.37%), Query Frame = 0


Query: 1   MAASSSSFFAAAFQKVGPAARRQILTLTDAAAARIRQLLQQRQRPFLRLGVKARGCNGLS 60
           MAASSSSFFAAA QK GPA RRQILTLT+AAAARIRQLLQQRQRPFLRLGVKARGCNGLS
Sbjct: 1   MAASSSSFFAAASQKAGPAVRRQILTLTEAAAARIRQLLQQRQRPFLRLGVKARGCNGLS 60

Query: 61  YTLNYAASQLTVLTIQFLMELLMIGKFSNSALPHYDLYIPEYFKIAVFHLTCPFSNISDE 120
           YTLNYA                                                    DE
Sbjct: 61  YTLNYA----------------------------------------------------DE 120

Query: 121 KGKFDELVEDQNVKILIDPKALMHVIGTKMDFVDDKLRSEFIFINPNSKGQCGCGESFMT 180
           KGKFDELVEDQNVKILIDPKALMHVIGTKMDFVDDKLRSEFIFINPNSKGQCGCGESFMT
Sbjct: 121 KGKFDELVEDQNVKILIDPKALMHVIGTKMDFVDDKLRSEFIFINPNSKGQCGCGESFMT 137

Query: 181 TGSAEANKR 190
           T SAEANK+
Sbjct: 181 TSSAEANKK 137

[Close]

BLAST of Cla97C05G099750 vs. TAIR 10
Match: AT2G16710.3 (Iron-sulphur cluster biosynthesis family protein )

HSP 1 Score: 193.7 bits (491), Expect = 1.3e-49
Identity = 105/184 (57.07%), Postives = 123/184 (66.85%), Query Frame = 0


Query: 5   SSSFFAAAFQKVGPAARRQILTLTDAAAARIRQLLQQRQRPFLRLGVKARGCNGLSYTLN 64
           +S   AAA  +VGPA R+Q+LTLTD AA+R+  LLQQRQ+PFLRLGVKARGCNGLSYTLN
Sbjct: 3   ASQILAAAAARVGPALRKQVLTLTDEAASRVHHLLQQRQKPFLRLGVKARGCNGLSYTLN 62

Query: 65  YAASQLTVLTIQFLMELLMIGKFSNSALPHYDLYIPEYFKIAVFHLTCPFSNISDEKGKF 124
           YA                  GK S                             +DEKGKF
Sbjct: 63  YA------------------GKTSG----------------------------NDEKGKF 122

Query: 125 DELVEDQNVKILIDPKALMHVIGTKMDFVDDKLRSEFIFINPNSKGQCGCGESFMTTGSA 184
           DELVE++ V+IL++PKALMHVIGTKMDFVDDKLRSEF+FINPNS+GQCGCGESFMTT ++
Sbjct: 123 DELVEEKGVRILVEPKALMHVIGTKMDFVDDKLRSEFVFINPNSQGQCGCGESFMTTSTS 140

Query: 185 EANK 189
            A +
Sbjct: 183 SAKQ 140

[Close]

BLAST of Cla97C05G099750 vs. TAIR 10
Match: AT2G16710.1 (Iron-sulphur cluster biosynthesis family protein )

HSP 1 Score: 189.5 bits (480), Expect = 2.5e-48
Identity = 102/184 (55.43%), Postives = 119/184 (64.67%), Query Frame = 0


Query: 5   SSSFFAAAFQKVGPAARRQILTLTDAAAARIRQLLQQRQRPFLRLGVKARGCNGLSYTLN 64
           +S   AAA  +VGPA R+Q+LTLTD AA+R+  LLQQRQ+PFLRLGVKARGCNGLSYTLN
Sbjct: 3   ASQILAAAAARVGPALRKQVLTLTDEAASRVHHLLQQRQKPFLRLGVKARGCNGLSYTLN 62

Query: 65  YAASQLTVLTIQFLMELLMIGKFSNSALPHYDLYIPEYFKIAVFHLTCPFSNISDEKGKF 124
           YA                                                    DEKGKF
Sbjct: 63  YA----------------------------------------------------DEKGKF 122

Query: 125 DELVEDQNVKILIDPKALMHVIGTKMDFVDDKLRSEFIFINPNSKGQCGCGESFMTTGSA 184
           DELVE++ V+IL++PKALMHVIGTKMDFVDDKLRSEF+FINPNS+GQCGCGESFMTT ++
Sbjct: 123 DELVEEKGVRILVEPKALMHVIGTKMDFVDDKLRSEFVFINPNSQGQCGCGESFMTTSTS 134

Query: 185 EANK 189
            A +
Sbjct: 183 SAKQ 134

[Close]

BLAST of Cla97C05G099750 vs. TAIR 10
Match: AT2G36260.1 (Iron-sulphur cluster biosynthesis family protein )

HSP 1 Score: 151.4 bits (381), Expect = 7.4e-37
Identity = 82/161 (50.93%), Postives = 99/161 (61.49%), Query Frame = 0


Query: 21  RRQILTLTDAAAARIRQLLQQRQRPFLRLGVKARGCNGLSYTLNYAASQLTVLTIQFLME 80
           R+Q+L L+D AAARIRQLLQ +Q+PFLRL V+A+GCNGLSY LNYA              
Sbjct: 2   RKQVLALSDTAAARIRQLLQHQQKPFLRLAVEAKGCNGLSYVLNYA-------------- 61

Query: 81  LLMIGKFSNSALPHYDLYIPEYFKIAVFHLTCPFSNISDEKGKFDELVEDQNVKILIDPK 140
                                                  EKGKFDE+VE++ VKIL+DPK
Sbjct: 62  --------------------------------------QEKGKFDEVVEEKGVKILVDPK 109

Query: 141 ALMHVIGTKMDFVDDKLRSEFIFINPNSKGQCGCGESFMTT 182
           A+MHVIGT+MDFVDDKLRSEF+F+NPN+  +CGCGESF TT
Sbjct: 122 AVMHVIGTEMDFVDDKLRSEFVFVNPNAT-KCGCGESFTTT 109

[Close]

BLAST of Cla97C05G099750 vs. TAIR 10
Match: AT2G16710.2 (Iron-sulphur cluster biosynthesis family protein )

HSP 1 Score: 139.8 bits (351), Expect = 2.2e-33
Identity = 80/154 (51.95%), Postives = 92/154 (59.74%), Query Frame = 0


Query: 5   SSSFFAAAFQKVGPAARRQILTLTDAAAARIRQLLQQRQRPFLRLGVKARGCNGLSYTLN 64
           +S   AAA  +VGPA R+Q+LTLTD AA+R+  LLQQRQ+PFLRLGVKARGCNGLSYTLN
Sbjct: 3   ASQILAAAAARVGPALRKQVLTLTDEAASRVHHLLQQRQKPFLRLGVKARGCNGLSYTLN 62

Query: 65  YAASQLTVLTIQFLMELLMIGKFSNSALPHYDLYIPEYFKIAVFHLTCPFSNISDEKGKF 124
           YA                                                    DEKGKF
Sbjct: 63  YA----------------------------------------------------DEKGKF 104

Query: 125 DELVEDQNVKILIDPKALMHVIGTKMDFVDDKLR 159
           DELVE++ V+IL++PKALMHVIGTKMDFVDDKLR
Sbjct: 123 DELVEEKGVRILVEPKALMHVIGTKMDFVDDKLR 104

[Close]

BLAST of Cla97C05G099750 vs. TAIR 10
Match: AT1G10500.1 (chloroplast-localized ISCA-like protein )

HSP 1 Score: 53.9 bits (128), Expect = 1.6e-07
Identity = 45/176 (25.57%), Postives = 73/176 (41.48%), Query Frame = 0


Query: 4   SSSSFFAAAFQKVGPAARRQILTLTDAAAARIRQLLQQRQRPF-LRLGVKARGCNGLSYT 63
           S+S   A A + + PA     ++L++ A   + ++  +R     LR+GVK  GC+G+SYT
Sbjct: 56  SASVPAAPAMEGLKPA-----ISLSENALKHLSKMRSERGEDLCLRIGVKQGGCSGMSYT 115

Query: 64  LNYAASQLTVLTIQFLMELLMIGKFSNSALPHYDLYIPEYFKIAVFHLTCPFSNISDEKG 123
           ++                      F N A    D                          
Sbjct: 116 MD----------------------FENRANARPD-------------------------- 175

Query: 124 KFDELVEDQNVKILIDPKALMHVIGTKMDFVDDKLRSEFIFINPNSKGQCGCGESF 179
             D  +E Q   I+ DPK+++ + G ++D+ D  +   F F NPN+   CGCG+SF
Sbjct: 176 --DSTIEYQGFTIVCDPKSMLFLFGMQLDYSDALIGGGFSFSNPNATQTCGCGKSF 176

The following BLAST results are available for this feature:

BLAST of Cla97C05G099750 vs. NCBI nr


Match Name	E-value	Identity	Description
XP_038891787.1	8.5e-59	70.90	iron-sulfur assembly protein IscA-like 1, mitochondrial isoform X1 [Benincasa hi...	[more]
XP_022987453.1	2.5e-58	70.37	iron-sulfur assembly protein IscA-like 1, mitochondrial [Cucurbita maxima]	[more]
KAG7032017.1	3.2e-58	69.84	Iron-sulfur assembly protein IscA-like 1, mitochondrial [Cucurbita argyrosperma ...	[more]
XP_022956980.1	3.6e-57	69.31	iron-sulfur assembly protein IscA-like 1, mitochondrial [Cucurbita moschata]	[more]
XP_004135035.1	3.6e-57	69.31	iron-sulfur assembly protein IscA-like 1, mitochondrial [Cucumis sativus] >KGN52...	[more]

BLAST of Cla97C05G099750 vs. ExPASy Swiss-Prot


Match Name	E-value	Identity	Description
Q8LBM4	3.5e-47	55.43	Iron-sulfur assembly protein IscA-like 1, mitochondrial OS=Arabidopsis thaliana ...	[more]
Q8L8C0	1.0e-35	50.93	Iron-sulfur assembly protein IscA-like 3, mitochondrial OS=Arabidopsis thaliana ...	[more]
Q07821	7.3e-21	36.48	Iron-sulfur assembly protein 1 OS=Saccharomyces cerevisiae (strain ATCC 204508 /...	[more]
Q54VS1	4.7e-20	32.35	Iron-sulfur cluster assembly 1 homolog, mitochondrial OS=Dictyostelium discoideu...	[more]
Q8T3X9	6.2e-20	39.24	Iron-sulfur cluster assembly 1 homolog, mitochondrial OS=Drosophila melanogaster...	[more]

BLAST of Cla97C05G099750 vs. ExPASy TrEMBL


Match Name	E-value	Identity	Description
A0A6J1JJH4	1.2e-58	70.37	iron-sulfur assembly protein IscA-like 1, mitochondrial OS=Cucurbita maxima OX=3...	[more]
A0A5A7SVZ7	1.7e-57	69.31	Iron-sulfur assembly protein IscA-like 1 OS=Cucumis melo var. makuwa OX=1194695 ...	[more]
A0A0A0KTF0	1.7e-57	69.31	Fe-S_biosyn domain-containing protein OS=Cucumis sativus OX=3659 GN=Csa_5G614650...	[more]
A0A6J1GZ87	1.7e-57	69.31	iron-sulfur assembly protein IscA-like 1, mitochondrial OS=Cucurbita moschata OX...	[more]
A0A1S3BFV2	1.7e-57	69.31	iron-sulfur assembly protein IscA-like 1, mitochondrial OS=Cucumis melo OX=3656 ...	[more]

BLAST of Cla97C05G099750 vs. TAIR 10


Match Name	E-value	Identity	Description
AT2G16710.3	1.3e-49	57.07	Iron-sulphur cluster biosynthesis family protein	[more]
AT2G16710.1	2.5e-48	55.43	Iron-sulphur cluster biosynthesis family protein	[more]
AT2G36260.1	7.4e-37	50.93	Iron-sulphur cluster biosynthesis family protein	[more]
AT2G16710.2	2.2e-33	51.95	Iron-sulphur cluster biosynthesis family protein	[more]
AT1G10500.1	1.6e-07	25.57	chloroplast-localized ISCA-like protein	[more]

InterPro

Analysis Name: InterPro Annotations of Watermelon (97103) v2.5
Date Performed: 2022-01-31


IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
IPR000361	FeS cluster biogenesis	PFAM	PF01521	Fe-S_biosyn	coord: 118..176 e-value: 1.2E-8 score: 35.1
IPR035903	HesB-like domain superfamily	GENE3D	2.60.300.12		coord: 24..180 e-value: 3.6E-30 score: 106.2
IPR035903	HesB-like domain superfamily	SUPERFAMILY	89360	HesB-like domain	coord: 117..169
IPR035903	HesB-like domain superfamily	SUPERFAMILY	89360	HesB-like domain	coord: 21..67
IPR016092	FeS cluster insertion protein	TIGRFAM	TIGR00049	TIGR00049	coord: 117..179 e-value: 1.5E-19 score: 68.1
None	No IPR available	PANTHER	PTHR10072:SF60	IRON-SULFUR ASSEMBLY PROTEIN ISCA-LIKE 3, MITOCHONDRIAL	coord: 1..67
None	No IPR available	PANTHER	PTHR10072:SF60	IRON-SULFUR ASSEMBLY PROTEIN ISCA-LIKE 3, MITOCHONDRIAL	coord: 116..188
None	No IPR available	PANTHER	PTHR10072	IRON-SULFUR CLUSTER ASSEMBLY PROTEIN	coord: 1..67
None	No IPR available	PANTHER	PTHR10072	IRON-SULFUR CLUSTER ASSEMBLY PROTEIN	coord: 116..188
IPR017870	FeS cluster insertion, C-terminal, conserved site	PROSITE	PS01152	HESB	coord: 161..178

Relationships

The following mRNA feature(s) are a part of this gene:


Feature Name	Unique Name	Type
Cla97C05G099750.2	Cla97C05G099750.2	mRNA

GO Annotation

GO Assignments

This gene is annotated with the following GO terms.

Category	Term Accession	Term Name
biological_process	GO:0016226	iron-sulfur cluster assembly
biological_process	GO:0097428	protein maturation by iron-sulfur cluster transfer
cellular_component	GO:0005739	mitochondrion
molecular_function	GO:0051537	2 iron, 2 sulfur cluster binding
molecular_function	GO:0051536	iron-sulfur cluster binding