Homology
BLAST of Cla97C05G090910 vs. NCBI nr
Match:
XP_038905573.1 (dihydrofolate synthetase [Benincasa hispida])
HSP 1 Score: 957.2 bits (2473), Expect = 6.6e-275
Identity = 505/603 (83.75%), Postives = 520/603 (86.24%), Query Frame = 0
Query: 1 MNVFKYRHRSRPQILRTHLLNHFVGEFRS---RIGSKQCFGTHSEDQHMAEFMEYLDSLK 60
M +FKY H RPQIL T LLNHF GE S RIGSKQCF TH EDQHM EFMEYLDSLK
Sbjct: 1 MKIFKYHHHCRPQILGTLLLNHFRGECPSISVRIGSKQCFSTHPEDQHMTEFMEYLDSLK 60
Query: 61 NYEKLGVPRGAGTDSDDGFDLGRMTRLMERLGNPQSKFKAIHIAGTKGKGSTAAFLSNIL 120
NYEKLGVP+GAGTDSDDGFDLGRM RLMERLGNPQSKFKAIHIAGTKGKGSTAAFLSNIL
Sbjct: 61 NYEKLGVPKGAGTDSDDGFDLGRMRRLMERLGNPQSKFKAIHIAGTKGKGSTAAFLSNIL 120
Query: 121 RAEGYSVGCYTSPHIETIRERICLGRSGEMVSGKALNSLFKRNKEFFDQSVELENGRISH 180
RAEGYS GCYTSPHIETIRERI LGRSGEMVSGKALNSLFKRNKEFFDQSVELENGRISH
Sbjct: 121 RAEGYSFGCYTSPHIETIRERISLGRSGEMVSGKALNSLFKRNKEFFDQSVELENGRISH 180
Query: 181 FEVLTAMAFSLFAEENVDVAVIEAGLGGARDATNIICSSRLAAAVITSIGEEHMAALGGS 240
FEVLTAMAF LFA+ENVD+AVIEAGLGGARDATNIICSS LAAAVITSIGEEHMAALGGS
Sbjct: 181 FEVLTAMAFLLFAQENVDLAVIEAGLGGARDATNIICSSGLAAAVITSIGEEHMAALGGS 240
Query: 241 LESIATAKAGIIKRGCPTILGGPFLPSIERILRDKALSMSSPVISASDPGNKSTTKGVSL 300
LESIATAKAGIIKRGCP ILGGPFLPSIERILRDKALSMSSPVISAS PGN+ST KGVSL
Sbjct: 241 LESIATAKAGIIKRGCPAILGGPFLPSIERILRDKALSMSSPVISASGPGNRSTIKGVSL 300
Query: 301 LNGGLCQCCDIVIQIDNEFIELLDVNLRMLGHHQLQNAATATCVILTLRNLGIISFCIFD 360
LNGG+CQCCD+VIQID+EFIELLDVNLRMLGHHQLQNAATATCVIL LRNLG
Sbjct: 301 LNGGICQCCDVVIQIDDEFIELLDVNLRMLGHHQLQNAATATCVILALRNLG-------- 360
Query: 361 LISVPSSENKHVMCYDFPGMFTEENSWSLLHLHFLFEVSEQIVPFACWRISDASVRSGLE 420
WRISDASVRSGLE
Sbjct: 361 -----------------------------------------------WRISDASVRSGLE 420
Query: 421 QTFLVGRSHFLPAKEAGILGLPGATILLDGAHTKDSAKALVDTIQMAFPEARLALVVAMA 480
QTFLVGRSHFLPAKEAG+LGLPGATILLDGAHTKDSAKALVDTIQMAFPEARLALVVAMA
Sbjct: 421 QTFLVGRSHFLPAKEAGVLGLPGATILLDGAHTKDSAKALVDTIQMAFPEARLALVVAMA 480
Query: 481 SDKDHIGFAREFLQGGKLESVVLTEAHIGGGKARTTSAAFLRECWIQASDELGIPISLET 540
SDKDHIGFAREFLQGGKLES+VLTEAHIGGGK+RTTSA FLR+CWIQAS+ELGIPISLET
Sbjct: 481 SDKDHIGFAREFLQGGKLESIVLTEAHIGGGKSRTTSAVFLRDCWIQASNELGIPISLET 540
Query: 541 KDALVSFTSKLENRPVLTTETSLLHAIIIAAEILKQRTEGRQGLVIVTGSLHAVSMVLSS 600
KDA VSFTSKLENRPVLTTETSLLHAI +AAEILKQRTEGRQGLV+VTGSLHAVSMVLS
Sbjct: 541 KDAPVSFTSKLENRPVLTTETSLLHAIKVAAEILKQRTEGRQGLVVVTGSLHAVSMVLSF 548
BLAST of Cla97C05G090910 vs. NCBI nr
Match:
XP_008465388.1 (PREDICTED: dihydrofolate synthetase isoform X1 [Cucumis melo])
HSP 1 Score: 933.3 bits (2411), Expect = 1.0e-267
Identity = 493/603 (81.76%), Postives = 515/603 (85.41%), Query Frame = 0
Query: 1 MNVFKYRHRSRPQILRTHLLNHFVGE---FRSRIGSKQCFGTHSEDQHMAEFMEYLDSLK 60
MNVFKY H RPQIL + LLNHF+GE SR GSKQCF THSEDQHM EFMEYLDSLK
Sbjct: 1 MNVFKYHHHCRPQILGSLLLNHFIGECPSISSRNGSKQCFATHSEDQHMTEFMEYLDSLK 60
Query: 61 NYEKLGVPRGAGTDSDDGFDLGRMTRLMERLGNPQSKFKAIHIAGTKGKGSTAAFLSNIL 120
NYEKLGVPRGAGTDSDDGFDLGRM RLMERLGNPQSKFKAIHIAGTKGKGS AAFLSNIL
Sbjct: 61 NYEKLGVPRGAGTDSDDGFDLGRMRRLMERLGNPQSKFKAIHIAGTKGKGSIAAFLSNIL 120
Query: 121 RAEGYSVGCYTSPHIETIRERICLGRSGEMVSGKALNSLFKRNKEFFDQSVELENGRISH 180
RAEGYSVGCYTSPHIET+RERI LGRSGEMVSGKALNSLFKRNKE FDQSVELE+GRISH
Sbjct: 121 RAEGYSVGCYTSPHIETLRERISLGRSGEMVSGKALNSLFKRNKEVFDQSVELEHGRISH 180
Query: 181 FEVLTAMAFSLFAEENVDVAVIEAGLGGARDATNIICSSRLAAAVITSIGEEHMAALGGS 240
FEVLTAMAFSLFA+ENVDVAVIEAGLGGARDATNIICSS LAAAVITSIGEEHMAALGGS
Sbjct: 181 FEVLTAMAFSLFAQENVDVAVIEAGLGGARDATNIICSSGLAAAVITSIGEEHMAALGGS 240
Query: 241 LESIATAKAGIIKRGCPTILGGPFLPSIERILRDKALSMSSPVISASDPGNKSTTKGVSL 300
LESIA AKAGIIKRGCPTILGG FLP IE ILRDKALSMSSPVI+ SDPGN+ST KGV++
Sbjct: 241 LESIAMAKAGIIKRGCPTILGGSFLPRIEYILRDKALSMSSPVIAVSDPGNRSTIKGVNM 300
Query: 301 LNGGLCQCCDIVIQIDNEFIELLDVNLRMLGHHQLQNAATATCVILTLRNLGIISFCIFD 360
LNGGLCQCCDIVIQIDNEF+ELLDVNLRMLG HQLQNAATATCVILTLRNLG
Sbjct: 301 LNGGLCQCCDIVIQIDNEFVELLDVNLRMLGRHQLQNAATATCVILTLRNLG-------- 360
Query: 361 LISVPSSENKHVMCYDFPGMFTEENSWSLLHLHFLFEVSEQIVPFACWRISDASVRSGLE 420
WRISDAS+RSGLE
Sbjct: 361 -----------------------------------------------WRISDASIRSGLE 420
Query: 421 QTFLVGRSHFLPAKEAGILGLPGATILLDGAHTKDSAKALVDTIQMAFPEARLALVVAMA 480
QTFL+GRSHFL AKEA +LGLPGATILLDGAHTKDSAKALVDTIQMAFPEARLALV+AMA
Sbjct: 421 QTFLIGRSHFLAAKEAEVLGLPGATILLDGAHTKDSAKALVDTIQMAFPEARLALVIAMA 480
Query: 481 SDKDHIGFAREFLQGGKLESVVLTEAHIGGGKARTTSAAFLRECWIQASDELGIPISLET 540
SDK+H+ FAREFLQGGKLE VVLTEAHIGGGK+RTTSAAFLR+CWIQAS ELGIPISLET
Sbjct: 481 SDKNHVDFAREFLQGGKLECVVLTEAHIGGGKSRTTSAAFLRDCWIQASIELGIPISLET 540
Query: 541 KDALVSFTSKLENRPVLTTETSLLHAIIIAAEILKQRTEGRQGLVIVTGSLHAVSMVLSS 600
KDA VSFTSKLENRPVLTTETSLLHAI IAAEILKQRT+G+Q LV+V+GSLHAVSMVLSS
Sbjct: 541 KDAEVSFTSKLENRPVLTTETSLLHAIKIAAEILKQRTKGQQSLVVVSGSLHAVSMVLSS 548
BLAST of Cla97C05G090910 vs. NCBI nr
Match:
XP_004149457.1 (dihydrofolate synthetase isoform X2 [Cucumis sativus])
HSP 1 Score: 928.7 bits (2399), Expect = 2.5e-266
Identity = 490/603 (81.26%), Postives = 517/603 (85.74%), Query Frame = 0
Query: 1 MNVFKYRHRSRPQILRTHLLNHFVGE---FRSRIGSKQCFGTHSEDQHMAEFMEYLDSLK 60
MNVFKY H RPQIL + LLNHF+GE SR GSKQCF THSEDQHM +FMEYLDSLK
Sbjct: 1 MNVFKYHHHCRPQILGSLLLNHFIGECPSISSRFGSKQCFATHSEDQHMTQFMEYLDSLK 60
Query: 61 NYEKLGVPRGAGTDSDDGFDLGRMTRLMERLGNPQSKFKAIHIAGTKGKGSTAAFLSNIL 120
NYEKLGVPRG+GTDSDDGFDLGRM RLMERLGNPQS+FKAIHIAGTKGKGSTAAFLSNIL
Sbjct: 61 NYEKLGVPRGSGTDSDDGFDLGRMRRLMERLGNPQSRFKAIHIAGTKGKGSTAAFLSNIL 120
Query: 121 RAEGYSVGCYTSPHIETIRERICLGRSGEMVSGKALNSLFKRNKEFFDQSVELENGRISH 180
R EGYSVGCYTSPHIETIRERI LGRSG+MVSGKALNSLFKRNKE FDQSVELENG +SH
Sbjct: 121 RVEGYSVGCYTSPHIETIRERISLGRSGDMVSGKALNSLFKRNKEVFDQSVELENGHLSH 180
Query: 181 FEVLTAMAFSLFAEENVDVAVIEAGLGGARDATNIICSSRLAAAVITSIGEEHMAALGGS 240
FEVLTAMAFSLFA+E+VDVAVIEAGLGGARDATNIICSS LAAAVITSIGEEH+AALGGS
Sbjct: 181 FEVLTAMAFSLFAQEDVDVAVIEAGLGGARDATNIICSSELAAAVITSIGEEHVAALGGS 240
Query: 241 LESIATAKAGIIKRGCPTILGGPFLPSIERILRDKALSMSSPVISASDPGNKSTTKGVSL 300
LESIATAKAGIIKRGCPTILGGPFLP IE ILRDKALSMSSPVISASDPGN+ST KGV+L
Sbjct: 241 LESIATAKAGIIKRGCPTILGGPFLPRIEYILRDKALSMSSPVISASDPGNRSTIKGVNL 300
Query: 301 LNGGLCQCCDIVIQIDNEFIELLDVNLRMLGHHQLQNAATATCVILTLRNLGIISFCIFD 360
LNGGL QCCDIVIQIDNEFIELLDVNLRMLG HQLQNAATATCVILTLRNLG
Sbjct: 301 LNGGLSQCCDIVIQIDNEFIELLDVNLRMLGPHQLQNAATATCVILTLRNLG-------- 360
Query: 361 LISVPSSENKHVMCYDFPGMFTEENSWSLLHLHFLFEVSEQIVPFACWRISDASVRSGLE 420
WRISDAS+RSGLE
Sbjct: 361 -----------------------------------------------WRISDASIRSGLE 420
Query: 421 QTFLVGRSHFLPAKEAGILGLPGATILLDGAHTKDSAKALVDTIQMAFPEARLALVVAMA 480
QTFL+GRSHFL A+EA +LGLPGATILLDGAHTKDSAKAL+DTIQMAFPEA+LALVVAMA
Sbjct: 421 QTFLIGRSHFLAAREAEVLGLPGATILLDGAHTKDSAKALLDTIQMAFPEAQLALVVAMA 480
Query: 481 SDKDHIGFAREFLQGGKLESVVLTEAHIGGGKARTTSAAFLRECWIQASDELGIPISLET 540
SDK+H+GFAREFLQGGKLESVVLTEA IGGGK+RTTSAAFLR+CWIQAS+ELGIPISLET
Sbjct: 481 SDKNHVGFAREFLQGGKLESVVLTEALIGGGKSRTTSAAFLRDCWIQASNELGIPISLET 540
Query: 541 KDALVSFTSKLENRPVLTTETSLLHAIIIAAEILKQRTEGRQGLVIVTGSLHAVSMVLSS 600
KDA V FTSKL NRPVLTTETSLLHAI IAAEILKQRT+GRQGLV+V+GSLHAVSMVL+S
Sbjct: 541 KDAEVFFTSKLGNRPVLTTETSLLHAIKIAAEILKQRTKGRQGLVVVSGSLHAVSMVLAS 548
BLAST of Cla97C05G090910 vs. NCBI nr
Match:
XP_011651415.1 (dihydrofolate synthetase isoform X1 [Cucumis sativus] >KGN57917.2 hypothetical protein Csa_010822 [Cucumis sativus])
HSP 1 Score: 918.3 bits (2372), Expect = 3.4e-263
Identity = 484/596 (81.21%), Postives = 510/596 (85.57%), Query Frame = 0
Query: 1 MNVFKYRHRSRPQILRTHLLNHFVGE---FRSRIGSKQCFGTHSEDQHMAEFMEYLDSLK 60
MNVFKY H RPQIL + LLNHF+GE SR GSKQCF THSEDQHM +FMEYLDSLK
Sbjct: 1 MNVFKYHHHCRPQILGSLLLNHFIGECPSISSRFGSKQCFATHSEDQHMTQFMEYLDSLK 60
Query: 61 NYEKLGVPRGAGTDSDDGFDLGRMTRLMERLGNPQSKFKAIHIAGTKGKGSTAAFLSNIL 120
NYEKLGVPRG+GTDSDDGFDLGRM RLMERLGNPQS+FKAIHIAGTKGKGSTAAFLSNIL
Sbjct: 61 NYEKLGVPRGSGTDSDDGFDLGRMRRLMERLGNPQSRFKAIHIAGTKGKGSTAAFLSNIL 120
Query: 121 RAEGYSVGCYTSPHIETIRERICLGRSGEMVSGKALNSLFKRNKEFFDQSVELENGRISH 180
R EGYSVGCYTSPHIETIRERI LGRSG+MVSGKALNSLFKRNKE FDQSVELENG +SH
Sbjct: 121 RVEGYSVGCYTSPHIETIRERISLGRSGDMVSGKALNSLFKRNKEVFDQSVELENGHLSH 180
Query: 181 FEVLTAMAFSLFAEENVDVAVIEAGLGGARDATNIICSSRLAAAVITSIGEEHMAALGGS 240
FEVLTAMAFSLFA+E+VDVAVIEAGLGGARDATNIICSS LAAAVITSIGEEH+AALGGS
Sbjct: 181 FEVLTAMAFSLFAQEDVDVAVIEAGLGGARDATNIICSSELAAAVITSIGEEHVAALGGS 240
Query: 241 LESIATAKAGIIKRGCPTILGGPFLPSIERILRDKALSMSSPVISASDPGNKSTTKGVSL 300
LESIATAKAGIIKRGCPTILGGPFLP IE ILRDKALSMSSPVISASDPGN+ST KGV+L
Sbjct: 241 LESIATAKAGIIKRGCPTILGGPFLPRIEYILRDKALSMSSPVISASDPGNRSTIKGVNL 300
Query: 301 LNGGLCQCCDIVIQIDNEFIELLDVNLRMLGHHQLQNAATATCVILTLRNLGIISFCIFD 360
LNGGL QCCDIVIQIDNEFIELLDVNLRMLG HQLQNAATATCVILTLRNLG
Sbjct: 301 LNGGLSQCCDIVIQIDNEFIELLDVNLRMLGPHQLQNAATATCVILTLRNLG-------- 360
Query: 361 LISVPSSENKHVMCYDFPGMFTEENSWSLLHLHFLFEVSEQIVPFACWRISDASVRSGLE 420
WRISDAS+RSGLE
Sbjct: 361 -----------------------------------------------WRISDASIRSGLE 420
Query: 421 QTFLVGRSHFLPAKEAGILGLPGATILLDGAHTKDSAKALVDTIQMAFPEARLALVVAMA 480
QTFL+GRSHFL A+EA +LGLPGATILLDGAHTKDSAKAL+DTIQMAFPEA+LALVVAMA
Sbjct: 421 QTFLIGRSHFLAAREAEVLGLPGATILLDGAHTKDSAKALLDTIQMAFPEAQLALVVAMA 480
Query: 481 SDKDHIGFAREFLQGGKLESVVLTEAHIGGGKARTTSAAFLRECWIQASDELGIPISLET 540
SDK+H+GFAREFLQGGKLESVVLTEA IGGGK+RTTSAAFLR+CWIQAS+ELGIPISLET
Sbjct: 481 SDKNHVGFAREFLQGGKLESVVLTEALIGGGKSRTTSAAFLRDCWIQASNELGIPISLET 540
Query: 541 KDALVSFTSKLENRPVLTTETSLLHAIIIAAEILKQRTEGRQGLVIVTGSLHAVSM 594
KDA V FTSKL NRPVLTTETSLLHAI IAAEILKQRT+GRQGLV+V+GSLHAVSM
Sbjct: 541 KDAEVFFTSKLGNRPVLTTETSLLHAIKIAAEILKQRTKGRQGLVVVSGSLHAVSM 541
BLAST of Cla97C05G090910 vs. NCBI nr
Match:
XP_022141068.1 (dihydrofolate synthetase isoform X1 [Momordica charantia])
HSP 1 Score: 915.2 bits (2364), Expect = 2.9e-262
Identity = 488/603 (80.93%), Postives = 510/603 (84.58%), Query Frame = 0
Query: 1 MNVFKYRHRSRPQILRTHLLNHFVGEFRS---RIGSKQCFGTHSEDQHMAEFMEYLDSLK 60
MNVFKY R RPQIL LLN VGE S RIGSKQCF THSED + EFMEYLDSLK
Sbjct: 1 MNVFKYHRRYRPQILGGLLLNFLVGEGPSISCRIGSKQCFATHSEDLELTEFMEYLDSLK 60
Query: 61 NYEKLGVPRGAGTDSDDGFDLGRMTRLMERLGNPQSKFKAIHIAGTKGKGSTAAFLSNIL 120
NYEKLGVPRGAGTDSDDGFDLGRM RLMERLGNPQSKFKAIHIAGTKGKGSTAAFLSNIL
Sbjct: 61 NYEKLGVPRGAGTDSDDGFDLGRMRRLMERLGNPQSKFKAIHIAGTKGKGSTAAFLSNIL 120
Query: 121 RAEGYSVGCYTSPHIETIRERICLGRSGEMVSGKALNSLFKRNKEFFDQSVELENGRISH 180
RAEGYSVGCYTSPHIETIRERI LGRSGEMVSGKALNSLFKRNKEF DQSVE ENGRISH
Sbjct: 121 RAEGYSVGCYTSPHIETIRERISLGRSGEMVSGKALNSLFKRNKEFLDQSVEFENGRISH 180
Query: 181 FEVLTAMAFSLFAEENVDVAVIEAGLGGARDATNIICSSRLAAAVITSIGEEHMAALGGS 240
FEVLTAMAFSLFA+ENVDVAVIEAGLGGARDATNIICSSRLAAAVITSIGEEHMAALGGS
Sbjct: 181 FEVLTAMAFSLFAQENVDVAVIEAGLGGARDATNIICSSRLAAAVITSIGEEHMAALGGS 240
Query: 241 LESIATAKAGIIKRGCPTILGGPFLPSIERILRDKALSMSSPVISASDPGNKSTTKGVSL 300
LESIA AKAGIIK GCPTILGGPFLPSIE IL DKALSMSS VISAS PG++ST KGVSL
Sbjct: 241 LESIAMAKAGIIKHGCPTILGGPFLPSIECILHDKALSMSSYVISASYPGHRSTIKGVSL 300
Query: 301 LNGGLCQCCDIVIQIDNEFIELLDVNLRMLGHHQLQNAATATCVILTLRNLGIISFCIFD 360
L+G LCQCCDIVIQIDNEFIELLDVNLRMLG HQLQNAATATC ILTLRNLG
Sbjct: 301 LDGRLCQCCDIVIQIDNEFIELLDVNLRMLGRHQLQNAATATCAILTLRNLG-------- 360
Query: 361 LISVPSSENKHVMCYDFPGMFTEENSWSLLHLHFLFEVSEQIVPFACWRISDASVRSGLE 420
WRISD+S+++GLE
Sbjct: 361 -----------------------------------------------WRISDSSIKTGLE 420
Query: 421 QTFLVGRSHFLPAKEAGILGLPGATILLDGAHTKDSAKALVDTIQMAFPEARLALVVAMA 480
QTFLVGRSHFL AKEAG+LGLPGATILLDGAHTKDSAKALVDTIQM FPEARLALVVAMA
Sbjct: 421 QTFLVGRSHFLSAKEAGVLGLPGATILLDGAHTKDSAKALVDTIQMTFPEARLALVVAMA 480
Query: 481 SDKDHIGFAREFLQGGKLESVVLTEAHIGGGKARTTSAAFLRECWIQASDELGIPISLET 540
SDKDH+GFAREFLQGGKLES++LTEA+IGGGK+RTTSAA LR+CWIQA++ELGIPISLET
Sbjct: 481 SDKDHVGFAREFLQGGKLESIILTEANIGGGKSRTTSAAILRDCWIQAANELGIPISLET 540
Query: 541 KDALVSFTSKLENRPVLTTETSLLHAIIIAAEILKQRTEGRQGLVIVTGSLHAVSMVLSS 600
KDALVS TSKLE+RP+LTTETSLLHA+ IAAEILKQRTEGR GL +VTGSLHAVSMVLSS
Sbjct: 541 KDALVSSTSKLESRPILTTETSLLHAMKIAAEILKQRTEGRAGLTVVTGSLHAVSMVLSS 548
BLAST of Cla97C05G090910 vs. ExPASy Swiss-Prot
Match:
F4JYE9 (Dihydrofolate synthetase OS=Arabidopsis thaliana OX=3702 GN=DHFS PE=1 SV=1)
HSP 1 Score: 552.7 bits (1423), Expect = 4.9e-156
Identity = 318/563 (56.48%), Postives = 381/563 (67.67%), Query Frame = 0
Query: 41 SEDQHMAEFMEYLDSLKNYEKLGVPRGAGTDSDDGFDLGRMTRLMERLGNPQSKFKAIHI 100
+ED + +F+ +L+SLKNYEK GVP+GAGTDSDDGFDLGRM RLM RL NP K+K +H+
Sbjct: 40 TEDPELRDFVGFLESLKNYEKSGVPKGAGTDSDDGFDLGRMKRLMLRLRNPHYKYKVVHV 99
Query: 101 AGTKGKGSTAAFLSNILRAEGYSVGCYTSPHIETIRERICLGRSGEMVSGKALNSLFKRN 160
AGTKGKGST+AFLSNILRA GYSVGCY+SPHI +I+ERI +GE VS LN LF
Sbjct: 100 AGTKGKGSTSAFLSNILRAGGYSVGCYSSPHILSIKERISC--NGEPVSASTLNDLFYSV 159
Query: 161 KEFFDQSVELENGRISHFEVLTAMAFSLFAEENVDVAVIEAGLGGARDATNIICSSRLAA 220
K +QS++ ENG +SHFE+LT +AFSLF +ENVD+AVIEAGLGGARDATN+I SS LAA
Sbjct: 160 KPILEQSIQEENGSLSHFEILTGIAFSLFEKENVDIAVIEAGLGGARDATNVIESSNLAA 219
Query: 221 AVITSIGEEHMAALGGSLESIATAKAGIIKRGCPTILGGPFLPSIERILRDKALSMSSPV 280
+VIT+IGEEHMAALGGSLESIA AK+GIIK G P +LGGPFLP IE ILR KA S+SS V
Sbjct: 220 SVITTIGEEHMAALGGSLESIAEAKSGIIKHGRPVVLGGPFLPHIEGILRSKAASVSSSV 279
Query: 281 ISASDPGNKSTTKGVSLLNG-GLCQCCDIVIQ---IDNEFIELLDVNLRMLGHHQLQNAA 340
I AS+ G+ S+ KG+ NG GLCQ CDIVIQ D +EL DVNLRMLGHHQLQNA
Sbjct: 280 ILASNIGSSSSIKGIINKNGIGLCQSCDIVIQNEKDDQPIVELSDVNLRMLGHHQLQNAV 339
Query: 341 TATCVILTLRNLGIISFCIFDLISVPSSENKHVMCYDFPGMFTEENSWSLLHLHFLFEVS 400
TATCV L LR+ G
Sbjct: 340 TATCVSLCLRDQG----------------------------------------------- 399
Query: 401 EQIVPFACWRISDASVRSGLEQTFLVGRSHFLPAKEAGILGLPGATILLDGAHTKDSAKA 460
C R++D ++R GLE T L+GRS FL KEA L LPGAT+LLDGAHTK+SA+A
Sbjct: 400 -------CGRVTDEAIRIGLENTRLLGRSQFLTPKEAETLLLPGATVLLDGAHTKESARA 459
Query: 461 LVDTIQMAFPEARLALVVAMASDKDHIGFAREFLQGGKLESVVLTEAHIGGGKARTTSAA 520
L + I+ FPE RL VVAMASDKDH+ FA+E L G K E+V+LTEA IGGGK R+T ++
Sbjct: 460 LKEMIKKDFPEKRLVFVVAMASDKDHVSFAKELLSGLKPEAVILTEADIGGGKIRSTESS 519
Query: 521 FLRECWIQASDELGIPISLETKDALVSFTSKLENRPVLTTETSLLHAIIIAAEILK-QRT 580
L+E WI+A+DELG S+E EN+ V L ++ +A +IL T
Sbjct: 520 VLKESWIKAADELG-SRSMEAS----------ENKTV-------LGSLKLAYKILSDDTT 528
Query: 581 EGRQGLVIVTGSLHAVSMVLSSL 599
G+VIVTGSLH VS VL+SL
Sbjct: 580 SSDSGMVIVTGSLHIVSSVLASL 528
BLAST of Cla97C05G090910 vs. ExPASy Swiss-Prot
Match:
Q05865 (Dihydrofolate synthase/folylpolyglutamate synthase OS=Bacillus subtilis (strain 168) OX=224308 GN=folC PE=3 SV=2)
HSP 1 Score: 162.9 bits (411), Expect = 1.1e-38
Identity = 126/406 (31.03%), Postives = 193/406 (47.54%), Query Frame = 0
Query: 78 LGRMTRLMERLGNPQSKFKAIHIAGTKGKGSTAAFLSNILRAEGYSVGCYTSPHIETIRE 137
LGRM +LM RLG+P+ K +A H+AGT GKGST AF+ ++L+ GY+VG +TSP+I T E
Sbjct: 24 LGRMKQLMARLGHPEKKIRAFHVAGTNGKGSTVAFIRSMLQEAGYTVGTFTSPYIITFNE 83
Query: 138 RICLGRSGEMVSGKALNSLFKRNKEFFDQSVELENGRISHFEVLTAMAFSLFAE-ENVDV 197
RI + +G +S + +L + K + + E G+ + FE++TA AF FAE VD
Sbjct: 84 RISV--NGIPISDEEWTALVNQMKPHVEALDQTEYGQPTEFEIMTACAFLYFAEFHKVDF 143
Query: 198 AVIEAGLGGARDATNIICSSRLAAAVITSIGEEHMAALGGSLESIATAKAGIIKRGCPTI 257
+ E GLGG D+TN++ VITSIG +HM LG ++E IA KAGIIK G P I
Sbjct: 144 VIFETGLGGRFDSTNVV---EPLLTVITSIGHDHMNILGNTIEEIAGEKAGIIKEGIP-I 203
Query: 258 LGGPFLPSIERILRDKALSMSSPVISASDPGNKSTTKGVSLLNGGLCQCCDIVIQIDNEF 317
+ P +++R +A ++P S D V L + + +
Sbjct: 204 VTAVTQPEALQVIRHEAERHAAPFQSLHD-------ACVIFNEEALPAGEQFSFKTEEKC 263
Query: 318 IELLDVNLRMLGHHQLQNAATATCVILTLRNLGIISFCIFDLISVPSSENKHVMCYDFPG 377
E D+ ++G HQ QNAA +S+ ++E
Sbjct: 264 YE--DIRTSLIGTHQRQNAA----------------------LSILAAE----------- 323
Query: 378 MFTEENSWSLLHLHFLFEVSEQIVPFACWRISDASVRSGLEQTFLVGRSHFLPAKEAGIL 437
+EN + H ISD ++RSGL + GR +
Sbjct: 324 WLNKEN---IAH------------------ISDEALRSGLVKAAWPGRLELVQEH----- 351
Query: 438 GLPGATILLDGAHTKDSAKALVDTIQMAFPEARLALVVAMASDKDH 483
+ LDGAH ++ + L +T++ F +R+++V + DK +
Sbjct: 384 ----PPVYLDGAHNEEGVEKLAETMKQRFANSRISVVFSALKDKPY 351
BLAST of Cla97C05G090910 vs. ExPASy Swiss-Prot
Match:
Q12676 (Dihydrofolate synthetase OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=FOL3 PE=1 SV=1)
HSP 1 Score: 124.4 bits (311), Expect = 4.3e-27
Identity = 86/270 (31.85%), Postives = 144/270 (53.33%), Query Frame = 0
Query: 78 LGRMTRLMERLGNPQSKFKAIHIAGTKGKGSTAAFLSNILRAEGYSVGCYTSPHIETIRE 137
L R+T+L+E LGNPQ+ + +HIAGT GKGS +LS++L+ + Y +G +T+PH+ + +
Sbjct: 7 LSRITKLLEHLGNPQNSLRVLHIAGTNGKGSVCTYLSSVLQQKSYQIGKFTTPHLVHVTD 66
Query: 138 RICLGRSGEMVSGKALNSLFKRNKEFFDQSVELENGRISHFEVLTAMAFSLFAEENVDVA 197
I + + + + + ++ + E ++S L + + FE+LT AF F +
Sbjct: 67 SITI--NNKPIPLERYQNI-RLQLEALNKSHSL---KCTEFELLTCTAFKYFYDVQCQWC 126
Query: 198 VIEAGLGGARDATNIICSSRLAAAVITSIGEEHMAALGGSLESIATAKAGIIKRGCP-TI 257
VIE GLGG DATN+I + A IT I +H + LG +L I+ KAGII G P T+
Sbjct: 127 VIEVGLGGRLDATNVIPGANKACCGITKISLDHESFLGNTLSEISKEKAGIITEGVPFTV 186
Query: 258 LGGPFLPSIERILRDKALSMSSPVISASDPGNKSTTKGVSLLNGGLCQ-----CCDIV-I 317
+ G S+ +++++ ++ S +S +D S LNG + C D+ +
Sbjct: 187 IDGTNEASVINVVKERCKALGSE-LSVTD----------SQLNGNMIDTNSWGCFDLAKL 246
Query: 318 QIDNEF-IELLDVNLRMLGHHQLQNAATAT 340
++ E+ I L V + ML + Q+ T
Sbjct: 247 PLNGEYQIFNLRVAMGMLDYLQMNELIDIT 259
BLAST of Cla97C05G090910 vs. ExPASy Swiss-Prot
Match:
P15925 (Folylpolyglutamate synthase OS=Lactobacillus casei OX=1582 GN=fpgS PE=1 SV=1)
HSP 1 Score: 120.6 bits (301), Expect = 6.3e-26
Identity = 72/203 (35.47%), Postives = 112/203 (55.17%), Query Frame = 0
Query: 77 DLGRMTRLMERLGNPQSKFKAIHIAGTKGKGSTAAFLSNILRAEGYSVGCYTSPHIETIR 136
D R+ L+ LGNPQ + + IH+ GT GKGS A ++++L A G +VG YTSP I
Sbjct: 21 DHRRILTLLHALGNPQQQGRYIHVTGTNGKGSAANAIAHVLEASGLTVGLYTSPFIMRFN 80
Query: 137 ERICLGRSGEMVSGKALNSLFKRNKEFFDQSVELE-NGRISHFEVLTAMAFSLFAEENVD 196
ERI + E + AL + + ++ + + + ++ FE +TA+ + F + VD
Sbjct: 81 ERIMIDH--EPIPDAALVNAVAFVRAALERLQQQQADFNVTEFEFITALGYWYFRQRQVD 140
Query: 197 VAVIEAGLGGARDATNIICSSRLAAAVITSIGEEHMAALGGSLESIATAKAGIIKRGCPT 256
VAVIE G+GG D+TN+I +V+T + +H LG ++ +IA KAGIIKRG P
Sbjct: 141 VAVIEVGIGGDTDSTNVITP---VVSVLTEVALDHQKLLGHTITAIAKHKAGIIKRGIPV 200
Query: 257 ILGGPFLPSIERILRDKALSMSS 279
+ G +P ++ K + S
Sbjct: 201 VTGN-LVPDAAAVVAAKVATTGS 217
BLAST of Cla97C05G090910 vs. ExPASy Swiss-Prot
Match:
A6H751 (Folylpolyglutamate synthase, mitochondrial OS=Bos taurus OX=9913 GN=FPGS PE=2 SV=1)
HSP 1 Score: 119.4 bits (298), Expect = 1.4e-25
Identity = 82/208 (39.42%), Postives = 118/208 (56.73%), Query Frame = 0
Query: 98 IHIAGTKGKGSTAAFLSNILRAEGYSVGCYTSPHIETIRERICLGRSGEMVSGKALNSLF 157
IHI GTKGKGST AF ILR+ G G ++SPH+ +RERI + +G+ +S + F
Sbjct: 96 IHITGTKGKGSTCAFTERILRSYGLKTGFFSSPHLVQVRERIRI--NGQPISSELFTKHF 155
Query: 158 KRNKEFFDQSVELENGRIS---HFEVLTAMAFSLFAEENVDVAVIEAGLGGARDATNIIC 217
R +++ + ++ +S +F LT MAF +F +E VD+AV+E G+GGA D TNII
Sbjct: 156 WRLYHRLEETKD-DSSCVSMPGYFRFLTLMAFHVFLQEKVDLAVLEVGIGGAYDCTNII- 215
Query: 218 SSRLAAAVITSIGEEHMAALGGSLESIATAKAGIIKRGCP--TILGGPFLPSIERILRDK 277
+ ITS+G +H LG ++E IA K GI K G P T+L P P +LRD+
Sbjct: 216 -RKPVVCGITSLGIDHTGLLGDTMEKIAWQKGGIFKSGVPAFTVL-QPDEPL--AVLRDR 275
Query: 278 ALSMSSPV-----ISASDPGNKSTTKGV 296
A +S P+ + A + G T G+
Sbjct: 276 AQQISCPLYLCPPLQALEEGGPPLTLGL 295
BLAST of Cla97C05G090910 vs. ExPASy TrEMBL
Match:
A0A1S3CNR7 (dihydrofolate synthetase isoform X1 OS=Cucumis melo OX=3656 GN=LOC103503019 PE=3 SV=1)
HSP 1 Score: 933.3 bits (2411), Expect = 5.0e-268
Identity = 493/603 (81.76%), Postives = 515/603 (85.41%), Query Frame = 0
Query: 1 MNVFKYRHRSRPQILRTHLLNHFVGE---FRSRIGSKQCFGTHSEDQHMAEFMEYLDSLK 60
MNVFKY H RPQIL + LLNHF+GE SR GSKQCF THSEDQHM EFMEYLDSLK
Sbjct: 1 MNVFKYHHHCRPQILGSLLLNHFIGECPSISSRNGSKQCFATHSEDQHMTEFMEYLDSLK 60
Query: 61 NYEKLGVPRGAGTDSDDGFDLGRMTRLMERLGNPQSKFKAIHIAGTKGKGSTAAFLSNIL 120
NYEKLGVPRGAGTDSDDGFDLGRM RLMERLGNPQSKFKAIHIAGTKGKGS AAFLSNIL
Sbjct: 61 NYEKLGVPRGAGTDSDDGFDLGRMRRLMERLGNPQSKFKAIHIAGTKGKGSIAAFLSNIL 120
Query: 121 RAEGYSVGCYTSPHIETIRERICLGRSGEMVSGKALNSLFKRNKEFFDQSVELENGRISH 180
RAEGYSVGCYTSPHIET+RERI LGRSGEMVSGKALNSLFKRNKE FDQSVELE+GRISH
Sbjct: 121 RAEGYSVGCYTSPHIETLRERISLGRSGEMVSGKALNSLFKRNKEVFDQSVELEHGRISH 180
Query: 181 FEVLTAMAFSLFAEENVDVAVIEAGLGGARDATNIICSSRLAAAVITSIGEEHMAALGGS 240
FEVLTAMAFSLFA+ENVDVAVIEAGLGGARDATNIICSS LAAAVITSIGEEHMAALGGS
Sbjct: 181 FEVLTAMAFSLFAQENVDVAVIEAGLGGARDATNIICSSGLAAAVITSIGEEHMAALGGS 240
Query: 241 LESIATAKAGIIKRGCPTILGGPFLPSIERILRDKALSMSSPVISASDPGNKSTTKGVSL 300
LESIA AKAGIIKRGCPTILGG FLP IE ILRDKALSMSSPVI+ SDPGN+ST KGV++
Sbjct: 241 LESIAMAKAGIIKRGCPTILGGSFLPRIEYILRDKALSMSSPVIAVSDPGNRSTIKGVNM 300
Query: 301 LNGGLCQCCDIVIQIDNEFIELLDVNLRMLGHHQLQNAATATCVILTLRNLGIISFCIFD 360
LNGGLCQCCDIVIQIDNEF+ELLDVNLRMLG HQLQNAATATCVILTLRNLG
Sbjct: 301 LNGGLCQCCDIVIQIDNEFVELLDVNLRMLGRHQLQNAATATCVILTLRNLG-------- 360
Query: 361 LISVPSSENKHVMCYDFPGMFTEENSWSLLHLHFLFEVSEQIVPFACWRISDASVRSGLE 420
WRISDAS+RSGLE
Sbjct: 361 -----------------------------------------------WRISDASIRSGLE 420
Query: 421 QTFLVGRSHFLPAKEAGILGLPGATILLDGAHTKDSAKALVDTIQMAFPEARLALVVAMA 480
QTFL+GRSHFL AKEA +LGLPGATILLDGAHTKDSAKALVDTIQMAFPEARLALV+AMA
Sbjct: 421 QTFLIGRSHFLAAKEAEVLGLPGATILLDGAHTKDSAKALVDTIQMAFPEARLALVIAMA 480
Query: 481 SDKDHIGFAREFLQGGKLESVVLTEAHIGGGKARTTSAAFLRECWIQASDELGIPISLET 540
SDK+H+ FAREFLQGGKLE VVLTEAHIGGGK+RTTSAAFLR+CWIQAS ELGIPISLET
Sbjct: 481 SDKNHVDFAREFLQGGKLECVVLTEAHIGGGKSRTTSAAFLRDCWIQASIELGIPISLET 540
Query: 541 KDALVSFTSKLENRPVLTTETSLLHAIIIAAEILKQRTEGRQGLVIVTGSLHAVSMVLSS 600
KDA VSFTSKLENRPVLTTETSLLHAI IAAEILKQRT+G+Q LV+V+GSLHAVSMVLSS
Sbjct: 541 KDAEVSFTSKLENRPVLTTETSLLHAIKIAAEILKQRTKGQQSLVVVSGSLHAVSMVLSS 548
BLAST of Cla97C05G090910 vs. ExPASy TrEMBL
Match:
A0A0A0L7A4 (Mur_ligase_M domain-containing protein OS=Cucumis sativus OX=3659 GN=Csa_3G354540 PE=3 SV=1)
HSP 1 Score: 928.7 bits (2399), Expect = 1.2e-266
Identity = 490/603 (81.26%), Postives = 517/603 (85.74%), Query Frame = 0
Query: 1 MNVFKYRHRSRPQILRTHLLNHFVGE---FRSRIGSKQCFGTHSEDQHMAEFMEYLDSLK 60
MNVFKY H RPQIL + LLNHF+GE SR GSKQCF THSEDQHM +FMEYLDSLK
Sbjct: 1 MNVFKYHHHCRPQILGSLLLNHFIGECPSISSRFGSKQCFATHSEDQHMTQFMEYLDSLK 60
Query: 61 NYEKLGVPRGAGTDSDDGFDLGRMTRLMERLGNPQSKFKAIHIAGTKGKGSTAAFLSNIL 120
NYEKLGVPRG+GTDSDDGFDLGRM RLMERLGNPQS+FKAIHIAGTKGKGSTAAFLSNIL
Sbjct: 61 NYEKLGVPRGSGTDSDDGFDLGRMRRLMERLGNPQSRFKAIHIAGTKGKGSTAAFLSNIL 120
Query: 121 RAEGYSVGCYTSPHIETIRERICLGRSGEMVSGKALNSLFKRNKEFFDQSVELENGRISH 180
R EGYSVGCYTSPHIETIRERI LGRSG+MVSGKALNSLFKRNKE FDQSVELENG +SH
Sbjct: 121 RVEGYSVGCYTSPHIETIRERISLGRSGDMVSGKALNSLFKRNKEVFDQSVELENGHLSH 180
Query: 181 FEVLTAMAFSLFAEENVDVAVIEAGLGGARDATNIICSSRLAAAVITSIGEEHMAALGGS 240
FEVLTAMAFSLFA+E+VDVAVIEAGLGGARDATNIICSS LAAAVITSIGEEH+AALGGS
Sbjct: 181 FEVLTAMAFSLFAQEDVDVAVIEAGLGGARDATNIICSSELAAAVITSIGEEHVAALGGS 240
Query: 241 LESIATAKAGIIKRGCPTILGGPFLPSIERILRDKALSMSSPVISASDPGNKSTTKGVSL 300
LESIATAKAGIIKRGCPTILGGPFLP IE ILRDKALSMSSPVISASDPGN+ST KGV+L
Sbjct: 241 LESIATAKAGIIKRGCPTILGGPFLPRIEYILRDKALSMSSPVISASDPGNRSTIKGVNL 300
Query: 301 LNGGLCQCCDIVIQIDNEFIELLDVNLRMLGHHQLQNAATATCVILTLRNLGIISFCIFD 360
LNGGL QCCDIVIQIDNEFIELLDVNLRMLG HQLQNAATATCVILTLRNLG
Sbjct: 301 LNGGLSQCCDIVIQIDNEFIELLDVNLRMLGPHQLQNAATATCVILTLRNLG-------- 360
Query: 361 LISVPSSENKHVMCYDFPGMFTEENSWSLLHLHFLFEVSEQIVPFACWRISDASVRSGLE 420
WRISDAS+RSGLE
Sbjct: 361 -----------------------------------------------WRISDASIRSGLE 420
Query: 421 QTFLVGRSHFLPAKEAGILGLPGATILLDGAHTKDSAKALVDTIQMAFPEARLALVVAMA 480
QTFL+GRSHFL A+EA +LGLPGATILLDGAHTKDSAKAL+DTIQMAFPEA+LALVVAMA
Sbjct: 421 QTFLIGRSHFLAAREAEVLGLPGATILLDGAHTKDSAKALLDTIQMAFPEAQLALVVAMA 480
Query: 481 SDKDHIGFAREFLQGGKLESVVLTEAHIGGGKARTTSAAFLRECWIQASDELGIPISLET 540
SDK+H+GFAREFLQGGKLESVVLTEA IGGGK+RTTSAAFLR+CWIQAS+ELGIPISLET
Sbjct: 481 SDKNHVGFAREFLQGGKLESVVLTEALIGGGKSRTTSAAFLRDCWIQASNELGIPISLET 540
Query: 541 KDALVSFTSKLENRPVLTTETSLLHAIIIAAEILKQRTEGRQGLVIVTGSLHAVSMVLSS 600
KDA V FTSKL NRPVLTTETSLLHAI IAAEILKQRT+GRQGLV+V+GSLHAVSMVL+S
Sbjct: 541 KDAEVFFTSKLGNRPVLTTETSLLHAIKIAAEILKQRTKGRQGLVVVSGSLHAVSMVLAS 548
BLAST of Cla97C05G090910 vs. ExPASy TrEMBL
Match:
A0A6J1CJG1 (dihydrofolate synthetase isoform X1 OS=Momordica charantia OX=3673 GN=LOC111011557 PE=3 SV=1)
HSP 1 Score: 915.2 bits (2364), Expect = 1.4e-262
Identity = 488/603 (80.93%), Postives = 510/603 (84.58%), Query Frame = 0
Query: 1 MNVFKYRHRSRPQILRTHLLNHFVGEFRS---RIGSKQCFGTHSEDQHMAEFMEYLDSLK 60
MNVFKY R RPQIL LLN VGE S RIGSKQCF THSED + EFMEYLDSLK
Sbjct: 1 MNVFKYHRRYRPQILGGLLLNFLVGEGPSISCRIGSKQCFATHSEDLELTEFMEYLDSLK 60
Query: 61 NYEKLGVPRGAGTDSDDGFDLGRMTRLMERLGNPQSKFKAIHIAGTKGKGSTAAFLSNIL 120
NYEKLGVPRGAGTDSDDGFDLGRM RLMERLGNPQSKFKAIHIAGTKGKGSTAAFLSNIL
Sbjct: 61 NYEKLGVPRGAGTDSDDGFDLGRMRRLMERLGNPQSKFKAIHIAGTKGKGSTAAFLSNIL 120
Query: 121 RAEGYSVGCYTSPHIETIRERICLGRSGEMVSGKALNSLFKRNKEFFDQSVELENGRISH 180
RAEGYSVGCYTSPHIETIRERI LGRSGEMVSGKALNSLFKRNKEF DQSVE ENGRISH
Sbjct: 121 RAEGYSVGCYTSPHIETIRERISLGRSGEMVSGKALNSLFKRNKEFLDQSVEFENGRISH 180
Query: 181 FEVLTAMAFSLFAEENVDVAVIEAGLGGARDATNIICSSRLAAAVITSIGEEHMAALGGS 240
FEVLTAMAFSLFA+ENVDVAVIEAGLGGARDATNIICSSRLAAAVITSIGEEHMAALGGS
Sbjct: 181 FEVLTAMAFSLFAQENVDVAVIEAGLGGARDATNIICSSRLAAAVITSIGEEHMAALGGS 240
Query: 241 LESIATAKAGIIKRGCPTILGGPFLPSIERILRDKALSMSSPVISASDPGNKSTTKGVSL 300
LESIA AKAGIIK GCPTILGGPFLPSIE IL DKALSMSS VISAS PG++ST KGVSL
Sbjct: 241 LESIAMAKAGIIKHGCPTILGGPFLPSIECILHDKALSMSSYVISASYPGHRSTIKGVSL 300
Query: 301 LNGGLCQCCDIVIQIDNEFIELLDVNLRMLGHHQLQNAATATCVILTLRNLGIISFCIFD 360
L+G LCQCCDIVIQIDNEFIELLDVNLRMLG HQLQNAATATC ILTLRNLG
Sbjct: 301 LDGRLCQCCDIVIQIDNEFIELLDVNLRMLGRHQLQNAATATCAILTLRNLG-------- 360
Query: 361 LISVPSSENKHVMCYDFPGMFTEENSWSLLHLHFLFEVSEQIVPFACWRISDASVRSGLE 420
WRISD+S+++GLE
Sbjct: 361 -----------------------------------------------WRISDSSIKTGLE 420
Query: 421 QTFLVGRSHFLPAKEAGILGLPGATILLDGAHTKDSAKALVDTIQMAFPEARLALVVAMA 480
QTFLVGRSHFL AKEAG+LGLPGATILLDGAHTKDSAKALVDTIQM FPEARLALVVAMA
Sbjct: 421 QTFLVGRSHFLSAKEAGVLGLPGATILLDGAHTKDSAKALVDTIQMTFPEARLALVVAMA 480
Query: 481 SDKDHIGFAREFLQGGKLESVVLTEAHIGGGKARTTSAAFLRECWIQASDELGIPISLET 540
SDKDH+GFAREFLQGGKLES++LTEA+IGGGK+RTTSAA LR+CWIQA++ELGIPISLET
Sbjct: 481 SDKDHVGFAREFLQGGKLESIILTEANIGGGKSRTTSAAILRDCWIQAANELGIPISLET 540
Query: 541 KDALVSFTSKLENRPVLTTETSLLHAIIIAAEILKQRTEGRQGLVIVTGSLHAVSMVLSS 600
KDALVS TSKLE+RP+LTTETSLLHA+ IAAEILKQRTEGR GL +VTGSLHAVSMVLSS
Sbjct: 541 KDALVSSTSKLESRPILTTETSLLHAMKIAAEILKQRTEGRAGLTVVTGSLHAVSMVLSS 548
BLAST of Cla97C05G090910 vs. ExPASy TrEMBL
Match:
A0A6J1E819 (dihydrofolate synthetase isoform X1 OS=Cucurbita moschata OX=3662 GN=LOC111430739 PE=3 SV=1)
HSP 1 Score: 901.0 bits (2327), Expect = 2.7e-258
Identity = 478/603 (79.27%), Postives = 506/603 (83.91%), Query Frame = 0
Query: 1 MNVFKYRHRSRPQILRTHLLNHFVGE---FRSRIGSKQCFGTHSEDQHMAEFMEYLDSLK 60
MN+FKY H RPQI LLN+FVGE SRIGSKQCF HSEDQ M EFMEYLDSLK
Sbjct: 1 MNLFKYHHHFRPQIHGRLLLNYFVGEGPSISSRIGSKQCFRNHSEDQDMTEFMEYLDSLK 60
Query: 61 NYEKLGVPRGAGTDSDDGFDLGRMTRLMERLGNPQSKFKAIHIAGTKGKGSTAAFLSNIL 120
NYEKLGVP GAGTDS+DGFDLGRM RLMERLGNPQSKFKAIHIAGTKGKGSTAAFLSNIL
Sbjct: 61 NYEKLGVPTGAGTDSEDGFDLGRMKRLMERLGNPQSKFKAIHIAGTKGKGSTAAFLSNIL 120
Query: 121 RAEGYSVGCYTSPHIETIRERICLGRSGEMVSGKALNSLFKRNKEFFDQSVELENGRISH 180
RAEGYSVGCYTSPHIETIRERI LGRSGEMVSGKALN LFKRNKE DQSV+LENGRISH
Sbjct: 121 RAEGYSVGCYTSPHIETIRERISLGRSGEMVSGKALNFLFKRNKELLDQSVKLENGRISH 180
Query: 181 FEVLTAMAFSLFAEENVDVAVIEAGLGGARDATNIICSSRLAAAVITSIGEEHMAALGGS 240
FEVLTAMAFSLFA+ENVDVAVIEAGLGGARDATNIICSSRLAAAVITSIGEEHMAALGGS
Sbjct: 181 FEVLTAMAFSLFAQENVDVAVIEAGLGGARDATNIICSSRLAAAVITSIGEEHMAALGGS 240
Query: 241 LESIATAKAGIIKRGCPTILGGPFLPSIERILRDKALSMSSPVISASDPGNKSTTKGVSL 300
LESIA AKAGIIK GCPTILGGPF+P+IE ILRDKALSMSSPV+SASDPGN+S+ KGVSL
Sbjct: 241 LESIAMAKAGIIKHGCPTILGGPFIPNIECILRDKALSMSSPVVSASDPGNRSSIKGVSL 300
Query: 301 LNGGLCQCCDIVIQIDNEFIELLDVNLRMLGHHQLQNAATATCVILTLRNLGIISFCIFD 360
LNG LCQCCD++IQ DNEFIEL DVNLRMLG HQLQNAATATCVILTLRNLG
Sbjct: 301 LNGRLCQCCDLIIQTDNEFIELFDVNLRMLGRHQLQNAATATCVILTLRNLG-------- 360
Query: 361 LISVPSSENKHVMCYDFPGMFTEENSWSLLHLHFLFEVSEQIVPFACWRISDASVRSGLE 420
WRIS+AS+RSGLE
Sbjct: 361 -----------------------------------------------WRISEASIRSGLE 420
Query: 421 QTFLVGRSHFLPAKEAGILGLPGATILLDGAHTKDSAKALVDTIQMAFPEARLALVVAMA 480
+TFLVGRSHFL AKEAGILGLPG ILLDGAHTKDSAKALVDTIQM+FP+A+LALVVAMA
Sbjct: 421 KTFLVGRSHFLSAKEAGILGLPGTAILLDGAHTKDSAKALVDTIQMSFPDAQLALVVAMA 480
Query: 481 SDKDHIGFAREFLQGGKLESVVLTEAHIGGGKARTTSAAFLRECWIQASDELGIPISLET 540
SDKDH GFA EFLQGGKLES+VL+EA+IGG K+RTTSAA LR+CWIQAS+E+GIPISLET
Sbjct: 481 SDKDHYGFATEFLQGGKLESIVLSEANIGGSKSRTTSAALLRDCWIQASNEMGIPISLET 540
Query: 541 KDALVSFTSKLENRPVLTTETSLLHAIIIAAEILKQRTEGRQGLVIVTGSLHAVSMVLSS 600
KDA VS TSKLENRPVLTTETSLL AI IAAEILKQR +GR+GLV+VTGSLHAVSMVLSS
Sbjct: 541 KDAPVSSTSKLENRPVLTTETSLLRAIKIAAEILKQRIDGRRGLVVVTGSLHAVSMVLSS 548
BLAST of Cla97C05G090910 vs. ExPASy TrEMBL
Match:
A0A5D3BTU4 (Dihydrofolate synthetase isoform X1 OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold11G00160 PE=3 SV=1)
HSP 1 Score: 870.5 bits (2248), Expect = 4.0e-249
Identity = 460/555 (82.88%), Postives = 480/555 (86.49%), Query Frame = 0
Query: 46 MAEFMEYLDSLKNYEKLGVPRGAGTDSDDGFDLGRMTRLMERLGNPQSKFKAIHIAGTKG 105
M EFMEYLDSLKNYEKLGVPRGAGTDSDDGFDLGRM RLMERLGNPQSKFKAIHIAGTKG
Sbjct: 1 MTEFMEYLDSLKNYEKLGVPRGAGTDSDDGFDLGRMRRLMERLGNPQSKFKAIHIAGTKG 60
Query: 106 KGSTAAFLSNILRAEGYSVGCYTSPHIETIRERICLGRSGEMVSGKALNSLFKRNKEFFD 165
KGS AAFLSNILRAEGYSVGCYTSPHIET+RERI LGRSGEMVSGKALNSLFKRNKE FD
Sbjct: 61 KGSIAAFLSNILRAEGYSVGCYTSPHIETLRERISLGRSGEMVSGKALNSLFKRNKEVFD 120
Query: 166 QSVELENGRISHFEVLTAMAFSLFAEENVDVAVIEAGLGGARDATNIICSSRLAAAVITS 225
QSVELE+GRISHFEVLTAMAFSLFA+ENVDVAVIEAGLGGARDATNIICSS LAAAVITS
Sbjct: 121 QSVELEHGRISHFEVLTAMAFSLFAQENVDVAVIEAGLGGARDATNIICSSGLAAAVITS 180
Query: 226 IGEEHMAALGGSLESIATAKAGIIKRGCPTILGGPFLPSIERILRDKALSMSSPVISASD 285
IGEEHMAALGGSLESIA AKAGIIKRGCPTILGG FLP IE ILRDKALSMSSPVI+ASD
Sbjct: 181 IGEEHMAALGGSLESIAMAKAGIIKRGCPTILGGSFLPRIEYILRDKALSMSSPVIAASD 240
Query: 286 PGNKSTTKGVSLLNGGLCQCCDIVIQIDNEFIELLDVNLRMLGHHQLQNAATATCVILTL 345
PGN+ST KGV++LNGGLCQCCDIVIQIDNEF+ELLDVNLRMLG HQLQNAATATCVILTL
Sbjct: 241 PGNRSTIKGVNMLNGGLCQCCDIVIQIDNEFVELLDVNLRMLGRHQLQNAATATCVILTL 300
Query: 346 RNLGIISFCIFDLISVPSSENKHVMCYDFPGMFTEENSWSLLHLHFLFEVSEQIVPFACW 405
RNLG W
Sbjct: 301 RNLG-------------------------------------------------------W 360
Query: 406 RISDASVRSGLEQTFLVGRSHFLPAKEAGILGLPGATILLDGAHTKDSAKALVDTIQMAF 465
RISDAS+RSGLEQTFL+GRSHFL AKEA +LGLPG TILLDGAHTKDSAKALVDTIQMAF
Sbjct: 361 RISDASIRSGLEQTFLIGRSHFLAAKEAEVLGLPGVTILLDGAHTKDSAKALVDTIQMAF 420
Query: 466 PEARLALVVAMASDKDHIGFAREFLQGGKLESVVLTEAHIGGGKARTTSAAFLRECWIQA 525
PEARLALV+AMASDK+H+ FAREFLQGGKLESVVLTEAHIGGGK+RTTSAAFLR+CWIQA
Sbjct: 421 PEARLALVIAMASDKNHVDFAREFLQGGKLESVVLTEAHIGGGKSRTTSAAFLRDCWIQA 480
Query: 526 SDELGIPISLETKDALVSFTSKLENRPVLTTETSLLHAIIIAAEILKQRTEGRQGLVIVT 585
S ELGIPISLETKDA VSFTSKLENRPVLTTETSLLHAI IAAEILKQRT+G+Q LV+V+
Sbjct: 481 SIELGIPISLETKDAEVSFTSKLENRPVLTTETSLLHAIKIAAEILKQRTKGQQSLVVVS 500
Query: 586 GSLHAVSMVLSSLHS 601
GSLHAVSMVLSSLHS
Sbjct: 541 GSLHAVSMVLSSLHS 500
BLAST of Cla97C05G090910 vs. TAIR 10
Match:
AT5G41480.1 (Folylpolyglutamate synthetase family protein )
HSP 1 Score: 552.7 bits (1423), Expect = 3.5e-157
Identity = 318/563 (56.48%), Postives = 381/563 (67.67%), Query Frame = 0
Query: 41 SEDQHMAEFMEYLDSLKNYEKLGVPRGAGTDSDDGFDLGRMTRLMERLGNPQSKFKAIHI 100
+ED + +F+ +L+SLKNYEK GVP+GAGTDSDDGFDLGRM RLM RL NP K+K +H+
Sbjct: 40 TEDPELRDFVGFLESLKNYEKSGVPKGAGTDSDDGFDLGRMKRLMLRLRNPHYKYKVVHV 99
Query: 101 AGTKGKGSTAAFLSNILRAEGYSVGCYTSPHIETIRERICLGRSGEMVSGKALNSLFKRN 160
AGTKGKGST+AFLSNILRA GYSVGCY+SPHI +I+ERI +GE VS LN LF
Sbjct: 100 AGTKGKGSTSAFLSNILRAGGYSVGCYSSPHILSIKERISC--NGEPVSASTLNDLFYSV 159
Query: 161 KEFFDQSVELENGRISHFEVLTAMAFSLFAEENVDVAVIEAGLGGARDATNIICSSRLAA 220
K +QS++ ENG +SHFE+LT +AFSLF +ENVD+AVIEAGLGGARDATN+I SS LAA
Sbjct: 160 KPILEQSIQEENGSLSHFEILTGIAFSLFEKENVDIAVIEAGLGGARDATNVIESSNLAA 219
Query: 221 AVITSIGEEHMAALGGSLESIATAKAGIIKRGCPTILGGPFLPSIERILRDKALSMSSPV 280
+VIT+IGEEHMAALGGSLESIA AK+GIIK G P +LGGPFLP IE ILR KA S+SS V
Sbjct: 220 SVITTIGEEHMAALGGSLESIAEAKSGIIKHGRPVVLGGPFLPHIEGILRSKAASVSSSV 279
Query: 281 ISASDPGNKSTTKGVSLLNG-GLCQCCDIVIQ---IDNEFIELLDVNLRMLGHHQLQNAA 340
I AS+ G+ S+ KG+ NG GLCQ CDIVIQ D +EL DVNLRMLGHHQLQNA
Sbjct: 280 ILASNIGSSSSIKGIINKNGIGLCQSCDIVIQNEKDDQPIVELSDVNLRMLGHHQLQNAV 339
Query: 341 TATCVILTLRNLGIISFCIFDLISVPSSENKHVMCYDFPGMFTEENSWSLLHLHFLFEVS 400
TATCV L LR+ G
Sbjct: 340 TATCVSLCLRDQG----------------------------------------------- 399
Query: 401 EQIVPFACWRISDASVRSGLEQTFLVGRSHFLPAKEAGILGLPGATILLDGAHTKDSAKA 460
C R++D ++R GLE T L+GRS FL KEA L LPGAT+LLDGAHTK+SA+A
Sbjct: 400 -------CGRVTDEAIRIGLENTRLLGRSQFLTPKEAETLLLPGATVLLDGAHTKESARA 459
Query: 461 LVDTIQMAFPEARLALVVAMASDKDHIGFAREFLQGGKLESVVLTEAHIGGGKARTTSAA 520
L + I+ FPE RL VVAMASDKDH+ FA+E L G K E+V+LTEA IGGGK R+T ++
Sbjct: 460 LKEMIKKDFPEKRLVFVVAMASDKDHVSFAKELLSGLKPEAVILTEADIGGGKIRSTESS 519
Query: 521 FLRECWIQASDELGIPISLETKDALVSFTSKLENRPVLTTETSLLHAIIIAAEILK-QRT 580
L+E WI+A+DELG S+E EN+ V L ++ +A +IL T
Sbjct: 520 VLKESWIKAADELG-SRSMEAS----------ENKTV-------LGSLKLAYKILSDDTT 528
Query: 581 EGRQGLVIVTGSLHAVSMVLSSL 599
G+VIVTGSLH VS VL+SL
Sbjct: 580 SSDSGMVIVTGSLHIVSSVLASL 528
BLAST of Cla97C05G090910 vs. TAIR 10
Match:
AT3G55630.3 (DHFS-FPGS homolog D )
HSP 1 Score: 115.5 bits (288), Expect = 1.4e-25
Identity = 95/272 (34.93%), Postives = 133/272 (48.90%), Query Frame = 0
Query: 39 THSEDQHMAEFMEYLDSLKN-YEKLGVPRGAGTDSDDGFDLGRMTRLMERLGNPQSKFKA 98
T + + A + LD+L + K G R A + F L + L + S+ K
Sbjct: 3 TEDDGELSARYQNTLDALSSLITKRG--RLASNNQSHRFRLLFHYLKVLELEDAVSQMKI 62
Query: 99 IHIAGTKGKGSTAAFLSNILRAEGYSVGCYTSPHIETIRERICLGRSGEMVSGKALN--- 158
IH+AGTKGKGST F +ILR G G +TSPH+ +RER L E+ K +N
Sbjct: 63 IHVAGTKGKGSTCTFAESILRCYGLRTGLFTSPHLIDVRERFRL-NGIEISQEKFVNYFW 122
Query: 159 SLFKRNKEFFDQSVELENGRISHFEVLTAMAFSLFAEENVDVAVIEAGLGGARDATNIIC 218
F + KE V + ++F L +AF +F E VDV ++E GLGG DATN+I
Sbjct: 123 CCFHKLKEKTSNEVPMP----TYFCFLALLAFKIFTTEQVDVVILEVGLGGRFDATNVI- 182
Query: 219 SSRLAAAVITSIGEEHMAALGGSLESIATAKAGIIKRGCPTI-LGGPFLPSIERILRDKA 278
+ I+S+G +HM LG +L IA KAGI K G P + P R+L +KA
Sbjct: 183 -QKPVVCGISSLGYDHMEILGYTLAEIAAEKAGIFKSGVPAFTVAQP--DEAMRVLNEKA 242
Query: 279 --LSMSSPVISASDPGNKSTTKGV-SLLNGGL 303
L ++ V+ D + +G LN GL
Sbjct: 243 SKLEVNLQVVEPLDSSQRLGLQGEHQYLNAGL 263
BLAST of Cla97C05G090910 vs. TAIR 10
Match:
AT5G05980.1 (DHFS-FPGS homolog B )
HSP 1 Score: 114.4 bits (285), Expect = 3.2e-25
Identity = 92/278 (33.09%), Postives = 130/278 (46.76%), Query Frame = 0
Query: 10 SRPQILRTHLLNHFVGEFRSRIGSKQCFGTHSEDQHMAEFME----YLDSLKNYEKLGVP 69
S P LR H H + + S F HS + + + Y ++L L
Sbjct: 24 SIPLTLRLH---HHYQHHQPHLPSPLSFQIHSLRKQIDMAAQGGDSYEEALAALSSLITK 83
Query: 70 RGAGTDSDDG------FDLGRMTRLMERLGNPQSKFKAIHIAGTKGKGSTAAFLSNILRA 129
R S+ G FD ++ L E + K IH+AGTKGKGST F +I+R
Sbjct: 84 RSRADKSNKGDRFELVFDYLKLLDLEEDI----LKMNVIHVAGTKGKGSTCTFTESIIRN 143
Query: 130 EGYSVGCYTSPHIETIRERICLGRSGEMVSGKALNSLF----KRNKEFFDQSVELENGRI 189
G+ G +TSPH+ +RER L G +S + F R KE ++ + +
Sbjct: 144 YGFRTGLFTSPHLIDVRERFRL--DGVDISEEKFLGYFWWCYNRLKERTNEEIPMP---- 203
Query: 190 SHFEVLTAMAFSLFAEENVDVAVIEAGLGGARDATNIICSSRLAAAVITSIGEEHMAALG 249
++F L +AF +FA E VD A++E GLGG DATN + + I+S+G +HM LG
Sbjct: 204 TYFRFLALLAFKIFAAEEVDAAILEVGLGGKFDATNAV--QKPVVCGISSLGYDHMEILG 263
Query: 250 GSLESIATAKAGIIKRGCPTILGGPFLPSIERILRDKA 274
+L IA KAGI K G P P R+L +KA
Sbjct: 264 DTLGKIAGEKAGIFKLGVPAFT-VPQPDEAMRVLEEKA 285
BLAST of Cla97C05G090910 vs. TAIR 10
Match:
AT3G55630.2 (DHFS-FPGS homolog D )
HSP 1 Score: 114.0 bits (284), Expect = 4.2e-25
Identity = 95/272 (34.93%), Postives = 132/272 (48.53%), Query Frame = 0
Query: 39 THSEDQHMAEFMEYLDSLKN-YEKLGVPRGAGTDSDDGFDLGRMTRLMERLGNPQSKFKA 98
T + + A + LD+L + K G R A + F L + L + S+ K
Sbjct: 3 TEDDGELSARYQNTLDALSSLITKRG--RLASNNQSHRFRLLFHYLKVLELEDAVSQMKI 62
Query: 99 IHIAGTKGKGSTAAFLSNILRAEGYSVGCYTSPHIETIRERICLGRSGEMVSGKALN--- 158
IH+AGTKGKGST F +ILR G G +TSPH+ +RER L E+ K +N
Sbjct: 63 IHVAGTKGKGSTCTFAESILRCYGLRTGLFTSPHLIDVRERFRL-NGIEISQEKFVNYFW 122
Query: 159 SLFKRNKEFFDQSVELENGRISHFEVLTAMAFSLFAEENVDVAVIEAGLGGARDATNIIC 218
F + KE V + ++F L +AF +F E VDV ++E GLGG DATNI
Sbjct: 123 CCFHKLKEKTSNEVPMP----TYFCFLALLAFKIFTTEQVDVVILEVGLGGRFDATNI-- 182
Query: 219 SSRLAAAVITSIGEEHMAALGGSLESIATAKAGIIKRGCPTI-LGGPFLPSIERILRDKA 278
+ I+S+G +HM LG +L IA KAGI K G P + P R+L +KA
Sbjct: 183 -QKPVVCGISSLGYDHMEILGYTLAEIAAEKAGIFKSGVPAFTVAQP--DEAMRVLNEKA 242
Query: 279 --LSMSSPVISASDPGNKSTTKGV-SLLNGGL 303
L ++ V+ D + +G LN GL
Sbjct: 243 SKLEVNLQVVEPLDSSQRLGLQGEHQYLNAGL 262
BLAST of Cla97C05G090910 vs. TAIR 10
Match:
AT5G05980.2 (DHFS-FPGS homolog B )
HSP 1 Score: 112.5 bits (280), Expect = 1.2e-24
Identity = 72/184 (39.13%), Postives = 99/184 (53.80%), Query Frame = 0
Query: 94 KFKAIHIAGTKGKGSTAAFLSNILRAEGYSVGCYTSPHIETIRERICLGRSGEMVSGKAL 153
K IH+AGTKGKGST F +I+R G+ G +TSPH+ +RER L G +S +
Sbjct: 53 KMNVIHVAGTKGKGSTCTFTESIIRNYGFRTGLFTSPHLIDVRERFRL--DGVDISEEKF 112
Query: 154 NSLF----KRNKEFFDQSVELENGRISHFEVLTAMAFSLFAEENVDVAVIEAGLGGARDA 213
F R KE ++ + + ++F L +AF +FA E VD A++E GLGG DA
Sbjct: 113 LGYFWWCYNRLKERTNEEIPMP----TYFRFLALLAFKIFAAEEVDAAILEVGLGGKFDA 172
Query: 214 TNIICSSRLAAAVITSIGEEHMAALGGSLESIATAKAGIIKRGCPTILGGPFLPSIERIL 273
TN + + I+S+G +HM LG +L IA KAGI K G P P R+L
Sbjct: 173 TNAV--QKPVVCGISSLGYDHMEILGDTLGKIAGEKAGIFKLGVPAFT-VPQPDEAMRVL 227
The following BLAST results are available for this feature:
Match Name | E-value | Identity | Description | |
XP_038905573.1 | 6.6e-275 | 83.75 | dihydrofolate synthetase [Benincasa hispida] | [more] |
XP_008465388.1 | 1.0e-267 | 81.76 | PREDICTED: dihydrofolate synthetase isoform X1 [Cucumis melo] | [more] |
XP_004149457.1 | 2.5e-266 | 81.26 | dihydrofolate synthetase isoform X2 [Cucumis sativus] | [more] |
XP_011651415.1 | 3.4e-263 | 81.21 | dihydrofolate synthetase isoform X1 [Cucumis sativus] >KGN57917.2 hypothetical p... | [more] |
XP_022141068.1 | 2.9e-262 | 80.93 | dihydrofolate synthetase isoform X1 [Momordica charantia] | [more] |
Match Name | E-value | Identity | Description | |
F4JYE9 | 4.9e-156 | 56.48 | Dihydrofolate synthetase OS=Arabidopsis thaliana OX=3702 GN=DHFS PE=1 SV=1 | [more] |
Q05865 | 1.1e-38 | 31.03 | Dihydrofolate synthase/folylpolyglutamate synthase OS=Bacillus subtilis (strain ... | [more] |
Q12676 | 4.3e-27 | 31.85 | Dihydrofolate synthetase OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c... | [more] |
P15925 | 6.3e-26 | 35.47 | Folylpolyglutamate synthase OS=Lactobacillus casei OX=1582 GN=fpgS PE=1 SV=1 | [more] |
A6H751 | 1.4e-25 | 39.42 | Folylpolyglutamate synthase, mitochondrial OS=Bos taurus OX=9913 GN=FPGS PE=2 SV... | [more] |
Match Name | E-value | Identity | Description | |
A0A1S3CNR7 | 5.0e-268 | 81.76 | dihydrofolate synthetase isoform X1 OS=Cucumis melo OX=3656 GN=LOC103503019 PE=3... | [more] |
A0A0A0L7A4 | 1.2e-266 | 81.26 | Mur_ligase_M domain-containing protein OS=Cucumis sativus OX=3659 GN=Csa_3G35454... | [more] |
A0A6J1CJG1 | 1.4e-262 | 80.93 | dihydrofolate synthetase isoform X1 OS=Momordica charantia OX=3673 GN=LOC1110115... | [more] |
A0A6J1E819 | 2.7e-258 | 79.27 | dihydrofolate synthetase isoform X1 OS=Cucurbita moschata OX=3662 GN=LOC11143073... | [more] |
A0A5D3BTU4 | 4.0e-249 | 82.88 | Dihydrofolate synthetase isoform X1 OS=Cucumis melo var. makuwa OX=1194695 GN=E5... | [more] |