Cla97C05G088880 (gene) Watermelon (97103) v2.5

BLAST of Cla97C05G088880 vs. NCBI nr
Match: XP_038891105.1 (DNA topoisomerase 3-beta isoform X1 [Benincasa hispida])

HSP 1 Score: 1745.3 bits (4519), Expect = 0.0e+00
Identity = 843/869 (97.01%), Postives = 855/869 (98.39%), Query Frame = 0

Query: 1   MVASTVLMVAEKPSIALSIASALSHGQMSTRKGSTDVHEFDGRFLGHHAHYKVTSVIGHV 60
           MVASTVLMVAEKPSIALSIASALSHGQMSTRKGSTDVHEFDGRFLGHHAHYKVTSVIGHV
Sbjct: 1   MVASTVLMVAEKPSIALSIASALSHGQMSTRKGSTDVHEFDGRFLGHHAHYKVTSVIGHV 60

Query: 61  FSIDFPAGFQDWKTTDPLDLFQASVIKTVSNPKAHIRQHLSREARGCDCLVLWLDCDREG 120
           FSIDFPAGFQDWK+TDPLDLFQASVIKT SNPKAHIRQHLSREARGCDCLVLWLDCDREG
Sbjct: 61  FSIDFPAGFQDWKSTDPLDLFQASVIKTESNPKAHIRQHLSREARGCDCLVLWLDCDREG 120

Query: 121 ENICFEVIECTGFGGNEIRKKVYRARFSSVTEKDILKALDNLVQPNRNEALAVDARQEID 180
           ENICFEVIECTGFGGNEIRKKVYRARFSSVTEKDILKALDNLVQPN+NEALAVDARQEID
Sbjct: 121 ENICFEVIECTGFGGNEIRKKVYRARFSSVTEKDILKALDNLVQPNKNEALAVDARQEID 180

Query: 181 LKVGVAFTRFQTTFFQGKYGNLDSRLISYGPCQTPTLGFCVQRHLQITTFKPEKFWSVCP 240
           LKVGVAFTRFQTTFFQGKYGNLDSRLISYGPCQTPTLGFCVQRHLQITTF PEKFWSVCP
Sbjct: 181 LKVGVAFTRFQTTFFQGKYGNLDSRLISYGPCQTPTLGFCVQRHLQITTFTPEKFWSVCP 240

Query: 241 YIMQNGYELNLDWERGKLFDCDVTAMFQKRVMEDGIAEVTEISEKQENKVRPSGLNTVNL 300
           YIMQNGYELNLDWERGKLFDCDVTAMFQKRV+EDGIAEVTEISEKQENKVRPSGLNTVNL
Sbjct: 241 YIMQNGYELNLDWERGKLFDCDVTAMFQKRVIEDGIAEVTEISEKQENKVRPSGLNTVNL 300

Query: 301 LKVASSALGFGPQMAMQLAERLYTQGFISYPRTESTAYPSSFDFKGTLHSLVKNPEWGDY 360
           LKVASSALGFGPQ+AMQLAERLYTQGFISYPRTESTAYPSSFDFKGTLH+L+KN EWGDY
Sbjct: 301 LKVASSALGFGPQIAMQLAERLYTQGFISYPRTESTAYPSSFDFKGTLHTLMKNSEWGDY 360

Query: 361 VRMLLSDGYSKPRSGTDVGDHPPITPMGLATEDMLGRDAWRLYQYVCQHFIGSLSSDCKY 420
           VRMLLSDGYSKPRSGTDVGDHPPITPM LATEDMLGRDAWRLYQYVCQHFIGSLSSDCKY
Sbjct: 361 VRMLLSDGYSKPRSGTDVGDHPPITPMCLATEDMLGRDAWRLYQYVCQHFIGSLSSDCKY 420

Query: 421 IRTKIQFSIAGEIFNCTGSRVMVKGFTSIMPWLAVNEKKLPQFLKGEKIEVARVELYQGN 480
           IRTKIQFSIAGE+FNCTGSRV+VKGFTSIMPWLAVNEKKLPQFLKGEKIEVARV+LYQGN
Sbjct: 421 IRTKIQFSIAGELFNCTGSRVIVKGFTSIMPWLAVNEKKLPQFLKGEKIEVARVDLYQGN 480

Query: 481 TTPPDYLSESELISLMEKHGIGTDASIPVHINNIGERNYVQVQAGRRLVPTNLGITLIRG 540
           TTPPDYLSESELISLMEKHGIGTDASIPVHINNIGERNYVQVQAGRRL PTNLGITL+RG
Sbjct: 481 TTPPDYLSESELISLMEKHGIGTDASIPVHINNIGERNYVQVQAGRRLAPTNLGITLVRG 540

Query: 541 YQCIDPDLCLPDIRSFIEQQITLVAKGQADHSSVVQNVLQQFKQKYSYFVKKIDHMDALF 600
           YQCIDPDLCLPDIRSFIEQQITLVAKGQADHSSVVQNVLQQFKQKY YFVKKIDHMDALF
Sbjct: 541 YQCIDPDLCLPDIRSFIEQQITLVAKGQADHSSVVQNVLQQFKQKYCYFVKKIDHMDALF 600

Query: 601 EAQFSPLADSGRALSKCGKCLRYMKFISSQPQRLYCGTCEEVYYLPQKGAIKLYKELTCP 660
           EAQFSPLADSGRALSKCGKCLRYMKFISSQPQRLYCG CEEVYYLPQKGAIKLYKELTCP
Sbjct: 601 EAQFSPLADSGRALSKCGKCLRYMKFISSQPQRLYCGICEEVYYLPQKGAIKLYKELTCP 660

Query: 661 LDNFELLIFSLPGPEGKSFPLCPYCYNSPPFEGINTLYGAAKTNPSSGASSKGMGMPCFL 720
           LDNFELLIFSLPGPEGKSFPLCPYCYNSPPFEGI+TLYGAAKTNPSSGASSKGMGMPCFL
Sbjct: 661 LDNFELLIFSLPGPEGKSFPLCPYCYNSPPFEGIDTLYGAAKTNPSSGASSKGMGMPCFL 720

Query: 721 CPHPTCRHSLITQGVCACPECSGTLVLDPVSAPNWKLNCNKCNCLVFLPQGAHRISTTNE 780
           C HPTCRHSLITQGVCACPECSGTLVLDPVSAPNWKLNCNKCNCLVFLPQGAHRIST NE
Sbjct: 721 CLHPTCRHSLITQGVCACPECSGTLVLDPVSAPNWKLNCNKCNCLVFLPQGAHRISTRNE 780

Query: 781 KCADCDSTIIEVDFNKKTTPLEDGATLFVGCILCDEFLHSLLEMKHGKSFFRGRGSRGRG 840
           KCADCDSTIIEVDFNKKTTPLE GATLFVGCILCDEFLHSLLEMKHGKS FRGRGS  RG
Sbjct: 781 KCADCDSTIIEVDFNKKTTPLEGGATLFVGCILCDEFLHSLLEMKHGKSLFRGRGS--RG 840

Query: 841 RGRGRGRGGRGRGRNSGKLMDPKMSFRDF 870
           RGRGRGRGGRGRGRNSG+ MDPKMSFRDF
Sbjct: 841 RGRGRGRGGRGRGRNSGRPMDPKMSFRDF 867

BLAST of Cla97C05G088880 vs. NCBI nr
Match: XP_023528530.1 (DNA topoisomerase 3-beta isoform X1 [Cucurbita pepo subsp. pepo])

HSP 1 Score: 1738.4 bits (4501), Expect = 0.0e+00
Identity = 835/869 (96.09%), Postives = 855/869 (98.39%), Query Frame = 0

Query: 1   MVASTVLMVAEKPSIALSIASALSHGQMSTRKGSTDVHEFDGRFLGHHAHYKVTSVIGHV 60
           MVAS+VLMVAEKPSIALSIASALSHGQMSTRKGST+VHEFDGRFLGHHAHYKVTSVIGHV
Sbjct: 1   MVASSVLMVAEKPSIALSIASALSHGQMSTRKGSTEVHEFDGRFLGHHAHYKVTSVIGHV 60

Query: 61  FSIDFPAGFQDWKTTDPLDLFQASVIKTVSNPKAHIRQHLSREARGCDCLVLWLDCDREG 120
           FSIDFPA FQDWK TDPLDLFQASVIK  SNPKAHIRQHLSREARGCDCLVLWLDCDREG
Sbjct: 61  FSIDFPARFQDWKATDPLDLFQASVIKAESNPKAHIRQHLSREARGCDCLVLWLDCDREG 120

Query: 121 ENICFEVIECTGFGGNEIRKKVYRARFSSVTEKDILKALDNLVQPNRNEALAVDARQEID 180
           ENICFEVIECTGFGGN+ RKKVYRARFSSVTEKDILKALDNLV+PNRNEALAVDARQEID
Sbjct: 121 ENICFEVIECTGFGGNDYRKKVYRARFSSVTEKDILKALDNLVEPNRNEALAVDARQEID 180

Query: 181 LKVGVAFTRFQTTFFQGKYGNLDSRLISYGPCQTPTLGFCVQRHLQITTFKPEKFWSVCP 240
           LKVGV+FTRFQTTFFQGKYGNLDSRLISYGPCQTPTLGFCVQRHLQITTFKPEKFWSVCP
Sbjct: 181 LKVGVSFTRFQTTFFQGKYGNLDSRLISYGPCQTPTLGFCVQRHLQITTFKPEKFWSVCP 240

Query: 241 YIMQNGYELNLDWERGKLFDCDVTAMFQKRVMEDGIAEVTEISEKQENKVRPSGLNTVNL 300
           YIMQNGYELNLDWERG+LFDCDV  MFQKRVMEDGIAEVT+ISEKQE+KVRPSGLNTVNL
Sbjct: 241 YIMQNGYELNLDWERGRLFDCDVATMFQKRVMEDGIAEVTQISEKQESKVRPSGLNTVNL 300

Query: 301 LKVASSALGFGPQMAMQLAERLYTQGFISYPRTESTAYPSSFDFKGTLHSLVKNPEWGDY 360
           LKVASSALG+GPQMAMQLAERLYTQGFISYPRTESTAYPSSFDFKGTLHSLVKNPEWGDY
Sbjct: 301 LKVASSALGYGPQMAMQLAERLYTQGFISYPRTESTAYPSSFDFKGTLHSLVKNPEWGDY 360

Query: 361 VRMLLSDGYSKPRSGTDVGDHPPITPMGLATEDMLGRDAWRLYQYVCQHFIGSLSSDCKY 420
           VRMLLSDGYSKPRSGTDVGDHPPITPM LATEDM+GRDAWRLYQYVCQHFIGSLSSDCKY
Sbjct: 361 VRMLLSDGYSKPRSGTDVGDHPPITPMSLATEDMVGRDAWRLYQYVCQHFIGSLSSDCKY 420

Query: 421 IRTKIQFSIAGEIFNCTGSRVMVKGFTSIMPWLAVNEKKLPQFLKGEKIEVARVELYQGN 480
           IRTKIQFSIAGEIFNCTGS VMVKGFTSIMPWLAVNEKKLPQFLKG+KIEVA+V+LYQGN
Sbjct: 421 IRTKIQFSIAGEIFNCTGSSVMVKGFTSIMPWLAVNEKKLPQFLKGQKIEVAKVDLYQGN 480

Query: 481 TTPPDYLSESELISLMEKHGIGTDASIPVHINNIGERNYVQVQAGRRLVPTNLGITLIRG 540
           T+PPDYLSESELISLMEKHGIGTDASIPVHINNIGERNYVQVQAGRRLVPTNLGITLIRG
Sbjct: 481 TSPPDYLSESELISLMEKHGIGTDASIPVHINNIGERNYVQVQAGRRLVPTNLGITLIRG 540

Query: 541 YQCIDPDLCLPDIRSFIEQQITLVAKGQADHSSVVQNVLQQFKQKYSYFVKKIDHMDALF 600
           YQCIDPDLCLPDIRSFIEQQIT VAKGQADH SVVQNVLQQFKQKYSYFVKKIDHMDALF
Sbjct: 541 YQCIDPDLCLPDIRSFIEQQITFVAKGQADHFSVVQNVLQQFKQKYSYFVKKIDHMDALF 600

Query: 601 EAQFSPLADSGRALSKCGKCLRYMKFISSQPQRLYCGTCEEVYYLPQKGAIKLYKELTCP 660
           EAQFSPLA+SGRALSKCGKCLRYMKFISSQPQRLYCGTCEEVYYLPQ+GAIKLYKELTCP
Sbjct: 601 EAQFSPLAESGRALSKCGKCLRYMKFISSQPQRLYCGTCEEVYYLPQRGAIKLYKELTCP 660

Query: 661 LDNFELLIFSLPGPEGKSFPLCPYCYNSPPFEGINTLYGAAKTNPSSGASSKGMGMPCFL 720
           LDNFELLIFSLPGPEGKSFPLCPYCYNSPPFEGI+TLYGAAKTNPSSGASSKGMGMPCFL
Sbjct: 661 LDNFELLIFSLPGPEGKSFPLCPYCYNSPPFEGIDTLYGAAKTNPSSGASSKGMGMPCFL 720

Query: 721 CPHPTCRHSLITQGVCACPECSGTLVLDPVSAPNWKLNCNKCNCLVFLPQGAHRISTTNE 780
           CPHPTCRHSLITQGVCACPECSGTLVLDPV APNWKLNCNKCNCLVFLPQGAHRI+TTNE
Sbjct: 721 CPHPTCRHSLITQGVCACPECSGTLVLDPVGAPNWKLNCNKCNCLVFLPQGAHRIATTNE 780

Query: 781 KCADCDSTIIEVDFNKKTTPLEDGATLFVGCILCDEFLHSLLEMKHGKSFFRGRGSRGRG 840
           KCA+CDSTIIEVDFNKKTTPLE GATLFVGCILCDEFLHSLLEMKHGKSFFRGRGSRGRG
Sbjct: 781 KCAECDSTIIEVDFNKKTTPLEGGATLFVGCILCDEFLHSLLEMKHGKSFFRGRGSRGRG 840

Query: 841 RGRGRGRGGRGRGRNSGKLMDPKMSFRDF 870
           RGRGRGR GRGRGR+SGKLMDPKMSFRDF
Sbjct: 841 RGRGRGR-GRGRGRSSGKLMDPKMSFRDF 868

BLAST of Cla97C05G088880 vs. NCBI nr
Match: KAG6582102.1 (DNA topoisomerase 3-beta, partial [Cucurbita argyrosperma subsp. sororia] >KAG7018517.1 DNA topoisomerase 3-beta, partial [Cucurbita argyrosperma subsp. argyrosperma])

HSP 1 Score: 1736.9 bits (4497), Expect = 0.0e+00
Identity = 834/869 (95.97%), Postives = 854/869 (98.27%), Query Frame = 0

Query: 1   MVASTVLMVAEKPSIALSIASALSHGQMSTRKGSTDVHEFDGRFLGHHAHYKVTSVIGHV 60
           MVAS+VLMVAEKPSIALSIASALSHGQMSTRKGST+VHEFDGRFLGHHAHYKVTSVIGHV
Sbjct: 1   MVASSVLMVAEKPSIALSIASALSHGQMSTRKGSTEVHEFDGRFLGHHAHYKVTSVIGHV 60

Query: 61  FSIDFPAGFQDWKTTDPLDLFQASVIKTVSNPKAHIRQHLSREARGCDCLVLWLDCDREG 120
           FSIDFPA FQDWK TDPLDLFQASVIKT SNPKAHIRQHLSREARGCDCLVLWLDCDREG
Sbjct: 61  FSIDFPARFQDWKATDPLDLFQASVIKTESNPKAHIRQHLSREARGCDCLVLWLDCDREG 120

Query: 121 ENICFEVIECTGFGGNEIRKKVYRARFSSVTEKDILKALDNLVQPNRNEALAVDARQEID 180
           ENICFEVIECTGFGGNE RKKVYRARFSSVTEKDILKALDNLV+PNRNEALAVDARQEID
Sbjct: 121 ENICFEVIECTGFGGNEYRKKVYRARFSSVTEKDILKALDNLVEPNRNEALAVDARQEID 180

Query: 181 LKVGVAFTRFQTTFFQGKYGNLDSRLISYGPCQTPTLGFCVQRHLQITTFKPEKFWSVCP 240
           LKVGV+FTRFQTTFFQGKYGNLDSRLISYGPCQTPTLGFCVQRHLQITTFKPEKFWSVCP
Sbjct: 181 LKVGVSFTRFQTTFFQGKYGNLDSRLISYGPCQTPTLGFCVQRHLQITTFKPEKFWSVCP 240

Query: 241 YIMQNGYELNLDWERGKLFDCDVTAMFQKRVMEDGIAEVTEISEKQENKVRPSGLNTVNL 300
           YIMQNGYELNLDWERG+LFDCDV  MFQKRVMEDGIAEVT+ISEKQE+KVRPSGLNTVNL
Sbjct: 241 YIMQNGYELNLDWERGRLFDCDVATMFQKRVMEDGIAEVTQISEKQESKVRPSGLNTVNL 300

Query: 301 LKVASSALGFGPQMAMQLAERLYTQGFISYPRTESTAYPSSFDFKGTLHSLVKNPEWGDY 360
           LKVASSALG+GPQMAMQLAERLYTQGFISYPRTESTAYPSSFDFKGTLHSLVKNPEWGDY
Sbjct: 301 LKVASSALGYGPQMAMQLAERLYTQGFISYPRTESTAYPSSFDFKGTLHSLVKNPEWGDY 360

Query: 361 VRMLLSDGYSKPRSGTDVGDHPPITPMGLATEDMLGRDAWRLYQYVCQHFIGSLSSDCKY 420
           VRMLLSDGYSKPRSGTDVGDHPPITPM LATEDM+GRDAWRLYQYVCQHFIGSLSSDCKY
Sbjct: 361 VRMLLSDGYSKPRSGTDVGDHPPITPMSLATEDMVGRDAWRLYQYVCQHFIGSLSSDCKY 420

Query: 421 IRTKIQFSIAGEIFNCTGSRVMVKGFTSIMPWLAVNEKKLPQFLKGEKIEVARVELYQGN 480
           +RTKIQFSIAGEIFNCTGS VMVKGFTSIMPWLAVNEKKLPQFLKG+KIEVA+V+LYQGN
Sbjct: 421 LRTKIQFSIAGEIFNCTGSSVMVKGFTSIMPWLAVNEKKLPQFLKGQKIEVAKVDLYQGN 480

Query: 481 TTPPDYLSESELISLMEKHGIGTDASIPVHINNIGERNYVQVQAGRRLVPTNLGITLIRG 540
           T+PPDYLSESELISLMEKHGIGTDASIPVHINNIGERNYVQVQAGRRLVPTNLGITLIRG
Sbjct: 481 TSPPDYLSESELISLMEKHGIGTDASIPVHINNIGERNYVQVQAGRRLVPTNLGITLIRG 540

Query: 541 YQCIDPDLCLPDIRSFIEQQITLVAKGQADHSSVVQNVLQQFKQKYSYFVKKIDHMDALF 600
           YQCIDPDLCLPDIRSFIEQQIT VAKGQADH SVVQNVLQQFKQKYSYFVKKIDHMDALF
Sbjct: 541 YQCIDPDLCLPDIRSFIEQQITFVAKGQADHFSVVQNVLQQFKQKYSYFVKKIDHMDALF 600

Query: 601 EAQFSPLADSGRALSKCGKCLRYMKFISSQPQRLYCGTCEEVYYLPQKGAIKLYKELTCP 660
           EAQFSPLA+SGRALSKCGKCLRYMKFISSQPQRLYCGTCEEVYYLPQ+GAIKLYKELTCP
Sbjct: 601 EAQFSPLAESGRALSKCGKCLRYMKFISSQPQRLYCGTCEEVYYLPQRGAIKLYKELTCP 660

Query: 661 LDNFELLIFSLPGPEGKSFPLCPYCYNSPPFEGINTLYGAAKTNPSSGASSKGMGMPCFL 720
           LDNFELLIFSLPGPEGKSFPLCPYCYNSPPFEGI+TLYGAAKTNPSSGASSKGMGMPCFL
Sbjct: 661 LDNFELLIFSLPGPEGKSFPLCPYCYNSPPFEGIDTLYGAAKTNPSSGASSKGMGMPCFL 720

Query: 721 CPHPTCRHSLITQGVCACPECSGTLVLDPVSAPNWKLNCNKCNCLVFLPQGAHRISTTNE 780
           CPHPTCRHSLITQGVCACPECSGTLVLDPV APNWKLNCNKCNCLVFLPQGAHRI+TTNE
Sbjct: 721 CPHPTCRHSLITQGVCACPECSGTLVLDPVGAPNWKLNCNKCNCLVFLPQGAHRIATTNE 780

Query: 781 KCADCDSTIIEVDFNKKTTPLEDGATLFVGCILCDEFLHSLLEMKHGKSFFRGRGSRGRG 840
           KCA+CDSTIIEVDFNKKTTPLE GATLFVGCILCDEFLHSLLEMKHGKSFFRGRGSRGRG
Sbjct: 781 KCAECDSTIIEVDFNKKTTPLEGGATLFVGCILCDEFLHSLLEMKHGKSFFRGRGSRGRG 840

Query: 841 RGRGRGRGGRGRGRNSGKLMDPKMSFRDF 870
           RGRGR   GRGRGR+SGKLMDPKMSFRDF
Sbjct: 841 RGRGR---GRGRGRSSGKLMDPKMSFRDF 866

BLAST of Cla97C05G088880 vs. NCBI nr
Match: XP_022955609.1 (DNA topoisomerase 3-beta isoform X1 [Cucurbita moschata])

HSP 1 Score: 1735.3 bits (4493), Expect = 0.0e+00
Identity = 833/869 (95.86%), Postives = 854/869 (98.27%), Query Frame = 0

Query: 1   MVASTVLMVAEKPSIALSIASALSHGQMSTRKGSTDVHEFDGRFLGHHAHYKVTSVIGHV 60
           MVAS+VLMVAEKPSIALSIASALSHGQMSTRKGST+VHEFDGRFLGHHAHYKVTSVIGHV
Sbjct: 1   MVASSVLMVAEKPSIALSIASALSHGQMSTRKGSTEVHEFDGRFLGHHAHYKVTSVIGHV 60

Query: 61  FSIDFPAGFQDWKTTDPLDLFQASVIKTVSNPKAHIRQHLSREARGCDCLVLWLDCDREG 120
           FSIDFPA FQDWK TDPLDLFQASVIKT SNPKAHIRQHLSREARGCDCLVLWLDCDREG
Sbjct: 61  FSIDFPARFQDWKATDPLDLFQASVIKTESNPKAHIRQHLSREARGCDCLVLWLDCDREG 120

Query: 121 ENICFEVIECTGFGGNEIRKKVYRARFSSVTEKDILKALDNLVQPNRNEALAVDARQEID 180
           ENICFEVIECTGFGGNE RKKVYRARFSSVTEKDILKALDNLV+PNRNEALAVDARQEID
Sbjct: 121 ENICFEVIECTGFGGNEYRKKVYRARFSSVTEKDILKALDNLVEPNRNEALAVDARQEID 180

Query: 181 LKVGVAFTRFQTTFFQGKYGNLDSRLISYGPCQTPTLGFCVQRHLQITTFKPEKFWSVCP 240
           LKVGV+FTRFQTTFFQGKYGNLDSRLISYGPCQTPTLGFCVQRHLQITTFKPEKFWSVCP
Sbjct: 181 LKVGVSFTRFQTTFFQGKYGNLDSRLISYGPCQTPTLGFCVQRHLQITTFKPEKFWSVCP 240

Query: 241 YIMQNGYELNLDWERGKLFDCDVTAMFQKRVMEDGIAEVTEISEKQENKVRPSGLNTVNL 300
           YIMQNGYELNLDWERG+LFDCDV  MFQKRVMEDGIAEVT+ISEKQE+KVRPSGLNTVNL
Sbjct: 241 YIMQNGYELNLDWERGRLFDCDVATMFQKRVMEDGIAEVTQISEKQESKVRPSGLNTVNL 300

Query: 301 LKVASSALGFGPQMAMQLAERLYTQGFISYPRTESTAYPSSFDFKGTLHSLVKNPEWGDY 360
           LKVASSALG+GPQMAMQLAERLYTQGFISYPRTESTAYPSSFDFKGTLHSLVKNPEWGDY
Sbjct: 301 LKVASSALGYGPQMAMQLAERLYTQGFISYPRTESTAYPSSFDFKGTLHSLVKNPEWGDY 360

Query: 361 VRMLLSDGYSKPRSGTDVGDHPPITPMGLATEDMLGRDAWRLYQYVCQHFIGSLSSDCKY 420
           VR+LLSDGYSKPRSGTDVGDHPPITPM LATEDM+GRDAWRLYQYVCQHFIGSLSSDCKY
Sbjct: 361 VRVLLSDGYSKPRSGTDVGDHPPITPMSLATEDMVGRDAWRLYQYVCQHFIGSLSSDCKY 420

Query: 421 IRTKIQFSIAGEIFNCTGSRVMVKGFTSIMPWLAVNEKKLPQFLKGEKIEVARVELYQGN 480
           +RTKIQFSIAGEIFNCTGS VMVKGFTSIMPWLAVNEKKLPQFLKG+KIEVA+V+LYQGN
Sbjct: 421 LRTKIQFSIAGEIFNCTGSSVMVKGFTSIMPWLAVNEKKLPQFLKGQKIEVAKVDLYQGN 480

Query: 481 TTPPDYLSESELISLMEKHGIGTDASIPVHINNIGERNYVQVQAGRRLVPTNLGITLIRG 540
           T+PPDYLSESELISLMEKHGIGTDASIPVHINNIGERNYVQVQAGRRLVPTNLGITLIRG
Sbjct: 481 TSPPDYLSESELISLMEKHGIGTDASIPVHINNIGERNYVQVQAGRRLVPTNLGITLIRG 540

Query: 541 YQCIDPDLCLPDIRSFIEQQITLVAKGQADHSSVVQNVLQQFKQKYSYFVKKIDHMDALF 600
           YQCIDPDLCLPDIRSFIEQQIT VAKGQADH SVVQNVLQQFKQKYSYFVKKIDHMDALF
Sbjct: 541 YQCIDPDLCLPDIRSFIEQQITFVAKGQADHFSVVQNVLQQFKQKYSYFVKKIDHMDALF 600

Query: 601 EAQFSPLADSGRALSKCGKCLRYMKFISSQPQRLYCGTCEEVYYLPQKGAIKLYKELTCP 660
           EAQFSPLA+SGRALSKCGKCLRYMKFISSQPQRLYCGTCEEVYYLPQ+GAIKLYKELTCP
Sbjct: 601 EAQFSPLAESGRALSKCGKCLRYMKFISSQPQRLYCGTCEEVYYLPQRGAIKLYKELTCP 660

Query: 661 LDNFELLIFSLPGPEGKSFPLCPYCYNSPPFEGINTLYGAAKTNPSSGASSKGMGMPCFL 720
           LDNFELLIFSLPGPEGKSFPLCPYCYNSPPFEGI+TLYGAAKTNPSSGASSKGMGMPCFL
Sbjct: 661 LDNFELLIFSLPGPEGKSFPLCPYCYNSPPFEGIDTLYGAAKTNPSSGASSKGMGMPCFL 720

Query: 721 CPHPTCRHSLITQGVCACPECSGTLVLDPVSAPNWKLNCNKCNCLVFLPQGAHRISTTNE 780
           CPHPTCRHSLITQGVCACPECSGTLVLDPV APNWKLNCNKCNCLVFLPQGAHRI+TTNE
Sbjct: 721 CPHPTCRHSLITQGVCACPECSGTLVLDPVGAPNWKLNCNKCNCLVFLPQGAHRIATTNE 780

Query: 781 KCADCDSTIIEVDFNKKTTPLEDGATLFVGCILCDEFLHSLLEMKHGKSFFRGRGSRGRG 840
           KCA+CDSTIIEVDFNKKTTPLE GATLFVGCILCDEFLHSLLEMKHGKSFFRGRGSRGRG
Sbjct: 781 KCAECDSTIIEVDFNKKTTPLEGGATLFVGCILCDEFLHSLLEMKHGKSFFRGRGSRGRG 840

Query: 841 RGRGRGRGGRGRGRNSGKLMDPKMSFRDF 870
           RGRGR   GRGRGR+SGKLMDPKMSFRDF
Sbjct: 841 RGRGR---GRGRGRSSGKLMDPKMSFRDF 866

BLAST of Cla97C05G088880 vs. NCBI nr
Match: XP_022979706.1 (DNA topoisomerase 3-beta isoform X1 [Cucurbita maxima])

HSP 1 Score: 1733.8 bits (4489), Expect = 0.0e+00
Identity = 833/869 (95.86%), Postives = 853/869 (98.16%), Query Frame = 0

Query: 1   MVASTVLMVAEKPSIALSIASALSHGQMSTRKGSTDVHEFDGRFLGHHAHYKVTSVIGHV 60
           MVAS+VLMVAEKPSIALSIASALSHGQMSTRKGST+VHEFDGRFLGHHAHYKVTSVIGHV
Sbjct: 1   MVASSVLMVAEKPSIALSIASALSHGQMSTRKGSTEVHEFDGRFLGHHAHYKVTSVIGHV 60

Query: 61  FSIDFPAGFQDWKTTDPLDLFQASVIKTVSNPKAHIRQHLSREARGCDCLVLWLDCDREG 120
           FSIDFPA FQDWK TDPLDLFQASVIKT SNPKAHIRQHLSREARGCDCLVLWLDCDREG
Sbjct: 61  FSIDFPARFQDWKATDPLDLFQASVIKTESNPKAHIRQHLSREARGCDCLVLWLDCDREG 120

Query: 121 ENICFEVIECTGFGGNEIRKKVYRARFSSVTEKDILKALDNLVQPNRNEALAVDARQEID 180
           ENICFEVIECTGFGGNE RKKVYRARFSSVTEKDILKALDNLV+PNRNEALAVDARQEID
Sbjct: 121 ENICFEVIECTGFGGNENRKKVYRARFSSVTEKDILKALDNLVEPNRNEALAVDARQEID 180

Query: 181 LKVGVAFTRFQTTFFQGKYGNLDSRLISYGPCQTPTLGFCVQRHLQITTFKPEKFWSVCP 240
           LKVGV+FTRFQTTFFQGKYGNLDSRLISYGPCQTPTLGFCVQRHLQITTFKPEKFWSVCP
Sbjct: 181 LKVGVSFTRFQTTFFQGKYGNLDSRLISYGPCQTPTLGFCVQRHLQITTFKPEKFWSVCP 240

Query: 241 YIMQNGYELNLDWERGKLFDCDVTAMFQKRVMEDGIAEVTEISEKQENKVRPSGLNTVNL 300
           YIMQNGYELNLDWERG+LFDCDV  MFQKRVMEDGIAEVT+ISEKQE+KVRPSGLNTVNL
Sbjct: 241 YIMQNGYELNLDWERGRLFDCDVATMFQKRVMEDGIAEVTQISEKQESKVRPSGLNTVNL 300

Query: 301 LKVASSALGFGPQMAMQLAERLYTQGFISYPRTESTAYPSSFDFKGTLHSLVKNPEWGDY 360
           LKVASSALG+GPQMAMQLAERLYTQGFISYPRTESTAYPSSFDFKGTLHSLVKNPEWGDY
Sbjct: 301 LKVASSALGYGPQMAMQLAERLYTQGFISYPRTESTAYPSSFDFKGTLHSLVKNPEWGDY 360

Query: 361 VRMLLSDGYSKPRSGTDVGDHPPITPMGLATEDMLGRDAWRLYQYVCQHFIGSLSSDCKY 420
           VRMLLSDGYSKPRSGTDVGDHPPITPM LATEDM+GRDAWRLYQYVCQHFIGSLSSDCKY
Sbjct: 361 VRMLLSDGYSKPRSGTDVGDHPPITPMSLATEDMVGRDAWRLYQYVCQHFIGSLSSDCKY 420

Query: 421 IRTKIQFSIAGEIFNCTGSRVMVKGFTSIMPWLAVNEKKLPQFLKGEKIEVARVELYQGN 480
           IRTKIQFSIAGEIFNCTGS V+VKGFTSIMPWLAVNEKKLPQFLKG+KIEVA+V+LYQGN
Sbjct: 421 IRTKIQFSIAGEIFNCTGSSVLVKGFTSIMPWLAVNEKKLPQFLKGQKIEVAKVDLYQGN 480

Query: 481 TTPPDYLSESELISLMEKHGIGTDASIPVHINNIGERNYVQVQAGRRLVPTNLGITLIRG 540
           T+PPDYLSESELISLMEKHGIGTDASIPVHINNIGERNYVQVQAGRRLVPTNLGITLIRG
Sbjct: 481 TSPPDYLSESELISLMEKHGIGTDASIPVHINNIGERNYVQVQAGRRLVPTNLGITLIRG 540

Query: 541 YQCIDPDLCLPDIRSFIEQQITLVAKGQADHSSVVQNVLQQFKQKYSYFVKKIDHMDALF 600
           YQCIDPDLCLPDIRSFIEQQIT VAKGQADH SVVQNVLQQFKQKYSYFVKKIDHMDALF
Sbjct: 541 YQCIDPDLCLPDIRSFIEQQITFVAKGQADHFSVVQNVLQQFKQKYSYFVKKIDHMDALF 600

Query: 601 EAQFSPLADSGRALSKCGKCLRYMKFISSQPQRLYCGTCEEVYYLPQKGAIKLYKELTCP 660
           EAQFSPLA+SGRALSKCGKCLRYMKFISSQPQRLYCGTCEEVYYLPQ+GAIKLYKELTCP
Sbjct: 601 EAQFSPLAESGRALSKCGKCLRYMKFISSQPQRLYCGTCEEVYYLPQRGAIKLYKELTCP 660

Query: 661 LDNFELLIFSLPGPEGKSFPLCPYCYNSPPFEGINTLYGAAKTNPSSGASSKGMGMPCFL 720
           LDNFELLIFSLPGPEGKSFPLCPYCYNSPPFEGI+ LYGAAKTNPSSGASSKGMGMPCFL
Sbjct: 661 LDNFELLIFSLPGPEGKSFPLCPYCYNSPPFEGIDALYGAAKTNPSSGASSKGMGMPCFL 720

Query: 721 CPHPTCRHSLITQGVCACPECSGTLVLDPVSAPNWKLNCNKCNCLVFLPQGAHRISTTNE 780
           CPHPTCRHSLITQGVCACPECSGTLVLDPV APNWKLNCNKCNCLVFLPQGAHRI+TTNE
Sbjct: 721 CPHPTCRHSLITQGVCACPECSGTLVLDPVGAPNWKLNCNKCNCLVFLPQGAHRIATTNE 780

Query: 781 KCADCDSTIIEVDFNKKTTPLEDGATLFVGCILCDEFLHSLLEMKHGKSFFRGRGSRGRG 840
           KCA+CDSTIIEVDFNKKTTPLE GATLFVGCILCDEFLHSLLEMKHGKSFFRGRGSRGRG
Sbjct: 781 KCAECDSTIIEVDFNKKTTPLEGGATLFVGCILCDEFLHSLLEMKHGKSFFRGRGSRGRG 840

Query: 841 RGRGRGRGGRGRGRNSGKLMDPKMSFRDF 870
           RGRGR   GRGRGR+SGKLMDPKMSFRDF
Sbjct: 841 RGRGR---GRGRGRSSGKLMDPKMSFRDF 866

BLAST of Cla97C05G088880 vs. ExPASy Swiss-Prot
Match: F4ISQ7 (DNA topoisomerase 3-beta OS=Arabidopsis thaliana OX=3702 GN=At2g32000 PE=2 SV=1)

HSP 1 Score: 1400.2 bits (3623), Expect = 0.0e+00
Identity = 662/864 (76.62%), Postives = 752/864 (87.04%), Query Frame = 0

Query: 6   VLMVAEKPSIALSIASALSHGQMSTRKGSTDVHEFDGRFLGHHAHYKVTSVIGHVFSIDF 65
           VLMVAEKPSIALSIAS LSHGQMSTR+GST+VHEFDG F G  AHY+VTSVIGHVFS+DF
Sbjct: 7   VLMVAEKPSIALSIASVLSHGQMSTRRGSTEVHEFDGMFRGFKAHYRVTSVIGHVFSVDF 66

Query: 66  PAGFQDWKTTDPLDLFQASVIKTVSNPKAHIRQHLSREARGCDCLVLWLDCDREGENICF 125
           P  +Q+W T DP DLF A +IK  SNPKAHI +HLS EARGC  +VLWLDCDREGENICF
Sbjct: 67  PEKYQNWATIDPQDLFDAPIIKKESNPKAHICRHLSNEARGCSYMVLWLDCDREGENICF 126

Query: 126 EVIECTGFGGNEIRKKVYRARFSSVTEKDILKALDNLVQPNRNEALAVDARQEIDLKVGV 185
           EVIE TGF   + ++KVYRARFSSVTEKDI KA+DNLV+PNR+EALAVDARQEIDLKVGV
Sbjct: 127 EVIESTGFDMKDSKRKVYRARFSSVTEKDISKAMDNLVEPNRDEALAVDARQEIDLKVGV 186

Query: 186 AFTRFQTTFFQGKYGNLDSRLISYGPCQTPTLGFCVQRHLQITTFKPEKFWSVCPYIMQN 245
           AF+RFQT++FQGKY NLD R+ISYGPCQTPTLGFCVQR++ I TFKPEKFW++ PYI ++
Sbjct: 187 AFSRFQTSYFQGKYQNLDCRVISYGPCQTPTLGFCVQRYMHINTFKPEKFWALRPYIRKD 246

Query: 246 GYELNLDWERGKLFDCDVTAMFQKRVMEDGIAEVTEISEKQENKVRPSGLNTVNLLKVAS 305
           GYEL L+WER +LFD +   +FQK V+E   A+V ++SEKQE K RP+GLNTVNLLKVAS
Sbjct: 247 GYELQLEWERRRLFDLEAATVFQKLVVEGRTAKVMDVSEKQEVKGRPAGLNTVNLLKVAS 306

Query: 306 SALGFGPQMAMQLAERLYTQGFISYPRTESTAYPSSFDFKGTLHSLVKNPEWGDYVRMLL 365
           SALGFGPQ AM LAERLYTQGFISYPRTESTAYPSSFDF  TL + V NP WG YV+ LL
Sbjct: 307 SALGFGPQTAMHLAERLYTQGFISYPRTESTAYPSSFDFTDTLRAQVSNPVWGGYVQRLL 366

Query: 366 SDGYSKPRSGTDVGDHPPITPMGLATEDMLGRDAWRLYQYVCQHFIGSLSSDCKYIRTKI 425
           SDG+  P+SGTD GDHPPITPM  ATE M+G DAWRLYQYVCQHF+G++S +CKYIRTK+
Sbjct: 367 SDGFHMPKSGTDAGDHPPITPMRAATEVMVGGDAWRLYQYVCQHFLGTVSPNCKYIRTKV 426

Query: 426 QFSIAGEIFNCTGSRVMVKGFTSIMPWLAVNEKKLPQFLKGEKIEVARVELYQGNTTPPD 485
           + SI GE F+CTG RV  KGFT+IMPW AV+EKKLP FLKGE+IEV RVELY+GNT PPD
Sbjct: 427 ELSIGGETFHCTGQRVTEKGFTAIMPWSAVDEKKLPSFLKGERIEVLRVELYEGNTAPPD 486

Query: 486 YLSESELISLMEKHGIGTDASIPVHINNIGERNYVQVQAGRRLVPTNLGITLIRGYQCID 545
           YL+ESELISLMEKHGIGTDASI VHINNIGERNYVQVQ+GR++VPT LGITLIRGYQCID
Sbjct: 487 YLTESELISLMEKHGIGTDASIAVHINNIGERNYVQVQSGRKMVPTALGITLIRGYQCID 546

Query: 546 PDLCLPDIRSFIEQQITLVAKGQADHSSVVQNVLQQFKQKYSYFVKKIDHMDALFEAQFS 605
           PDLCLPDIRSFIEQQITLVAKGQADHS VVQ+V+QQF++K+SYFV++I+HMDALFEAQFS
Sbjct: 547 PDLCLPDIRSFIEQQITLVAKGQADHSHVVQHVIQQFRRKFSYFVQQIEHMDALFEAQFS 606

Query: 606 PLADSGRALSKCGKCLRYMKFISSQPQRLYCGTCEEVYYLPQKGAIKLYKELTCPLDNFE 665
           PLADSGRALSKCGKCLRYMK I++ P RL+CGTCEEVYYLPQKG +KLYKELTCPLDNFE
Sbjct: 607 PLADSGRALSKCGKCLRYMKHITAVPPRLFCGTCEEVYYLPQKGTVKLYKELTCPLDNFE 666

Query: 666 LLIFSLPGPEGKSFPLCPYCYNSPPFEGINTLYGAAKTNPSSGASSKGMGMPCFLCPHPT 725
           L+I+S+PGPEGKSFPLCPYCYNSPPFEGI+TL+GA+KT  +   +  G GMPC LCPHPT
Sbjct: 667 LVIYSVPGPEGKSFPLCPYCYNSPPFEGIDTLFGASKTPNAPAKTKTGAGMPCSLCPHPT 726

Query: 726 CRHSLITQGVCACPECSGTLVLDPVSAPNWKLNCNKCNCLVFLPQGAHRISTTNEKCADC 785
           C+HS+  QGVCACPEC GTLVLDPVS P WKLNCN C+C+V LP+GAHRI+TT+ +C +C
Sbjct: 727 CQHSVRNQGVCACPECEGTLVLDPVSFPKWKLNCNLCSCIVLLPEGAHRITTTSNRCPEC 786

Query: 786 DSTIIEVDFNKKTTPLEDGATLFVGCILCDEFLHSLLEMKHGKSFFRGRGSRGRGRGRGR 845
           DS IIE+DFNKKTTPLE+GATL  GC+LCDE L SL+E+KHG+SF R RG RGRGRGRGR
Sbjct: 787 DSAIIEIDFNKKTTPLENGATLHQGCVLCDELLLSLVEVKHGRSFVR-RGGRGRGRGRGR 846

Query: 846 GRGGRGRGRNSGKLMDPKMSFRDF 870
           GRG    GR   K +DPKMSFRDF
Sbjct: 847 GRG----GRRGSKSVDPKMSFRDF 865

BLAST of Cla97C05G088880 vs. ExPASy Swiss-Prot
Match: Q0J0S6 (DNA topoisomerase 3-beta OS=Oryza sativa subsp. japonica OX=39947 GN=TOP3B PE=2 SV=1)

HSP 1 Score: 1349.0 bits (3490), Expect = 0.0e+00
Identity = 638/868 (73.50%), Postives = 741/868 (85.37%), Query Frame = 0

Query: 2   VASTVLMVAEKPSIALSIASALSHGQMSTRKGSTDVHEFDGRFLGHHAHYKVTSVIGHVF 61
           +A TVLMVAEKPSIALSIASALS G+MSTRKGSTDVHEFDG F G HA +KVTSVIGHV 
Sbjct: 1   MAPTVLMVAEKPSIALSIASALSGGRMSTRKGSTDVHEFDGMFQGSHAFFKVTSVIGHVL 60

Query: 62  SIDFPAGFQDWKTTDPLDLFQASVIKTVSNPKAHIRQHLSREARGCDCLVLWLDCDREGE 121
           S+DFP  +Q+W+ TDP+DLF A V+++  NPKAHIR+HL++EARGC  LVLWLDCDREGE
Sbjct: 61  SVDFPPAYQNWEGTDPMDLFVAPVLRSECNPKAHIRRHLAQEARGCTYLVLWLDCDREGE 120

Query: 122 NICFEVIECTGFGGNEIRKKVYRARFSSVTEKDILKALDNLVQPNRNEALAVDARQEIDL 181
           NIC+EVI+CTG   +E+ ++++RA+FSSVTEKDI+ A++NLV P+++EALAVDARQEIDL
Sbjct: 121 NICYEVIDCTGIPKSEVGRRIFRAKFSSVTEKDIMDAMNNLVLPSKDEALAVDARQEIDL 180

Query: 182 KVGVAFTRFQTTFFQGKYGNLDSRLISYGPCQTPTLGFCVQRHLQITTFKPEKFWSVCPY 241
           KVGVAFTRFQT +FQGKYGNLDSR+ISYGPCQTPTLGFCVQR+ QITTFKPEKFWS+  Y
Sbjct: 181 KVGVAFTRFQTRYFQGKYGNLDSRVISYGPCQTPTLGFCVQRYQQITTFKPEKFWSLKTY 240

Query: 242 IMQNGYELNLDWERGKLFDCDVTAMFQKRVMEDGIAEVTEISEKQENKVRPSGLNTVNLL 301
           ++++G E+ L+W+R KLFD DVT MFQK V  DGI +VT+IS K+E K RP GLNTVNLL
Sbjct: 241 VIKDGNEIQLEWDRKKLFDFDVTVMFQKMVASDGILKVTDISVKEECKARPPGLNTVNLL 300

Query: 302 KVASSALGFGPQMAMQLAERLYTQGFISYPRTESTAYPSSFDFKGTLHSLVKNPEWGDYV 361
           KVASSALG GPQ AM LAERLYTQGFISYPRTESTAYPSSFDF+  L +L  NP W + V
Sbjct: 301 KVASSALGIGPQTAMHLAERLYTQGFISYPRTESTAYPSSFDFRSALAALAHNPLWSNDV 360

Query: 362 RMLLSDGYSKPRSGTDVGDHPPITPMGLATEDMLGRDAWRLYQYVCQHFIGSLSSDCKYI 421
           R LL  G+ KP+ G D GDHPPITPM LATE+ LG DAWRLYQY+CQHFIG++S DC+Y 
Sbjct: 361 RTLLDTGFVKPKQGHDAGDHPPITPMRLATEEALGTDAWRLYQYICQHFIGTVSPDCRYT 420

Query: 422 RTKIQFSIAGEIFNCTGSRVMVKGFTSIMPWLAVNEKKLPQFLKGEKIEVARVELYQGNT 481
           RT I+F+  GE F+C G+RV  KGFTSIMPWLAV+E  +P + KG+ + + +V++Y+G+T
Sbjct: 421 RTSIEFTSGGETFHCVGNRVTSKGFTSIMPWLAVSENNIPAYKKGDAVSIHKVDIYEGST 480

Query: 482 TPPDYLSESELISLMEKHGIGTDASIPVHINNIGERNYVQVQAGRRLVPTNLGITLIRGY 541
           TPPDYLSESELISLMEK+GIGTDASIPVH+NNI ERNYVQV +GRRLVPT LG TLIRGY
Sbjct: 481 TPPDYLSESELISLMEKNGIGTDASIPVHVNNICERNYVQVNSGRRLVPTPLGTTLIRGY 540

Query: 542 QCIDPDLCLPDIRSFIEQQITLVAKGQADHSSVVQNVLQQFKQKYSYFVKKIDHMDALFE 601
           QCID DLCLPDIR FIEQQITL+AKG+ADH  VVQ+VLQQF +KYSYFVKKI++MDALFE
Sbjct: 541 QCIDADLCLPDIRRFIEQQITLIAKGEADHLQVVQHVLQQFMKKYSYFVKKIENMDALFE 600

Query: 602 AQFSPLADSGRALSKCGKCLRYMKFISSQPQRLYCGTCEEVYYLPQKGAIKLYKELTCPL 661
           AQFSPLADSGR LSKCGKC RYMK+IS+QP RLYC TCEEVYYLPQ G+IKLYKE+ CPL
Sbjct: 601 AQFSPLADSGRLLSKCGKCARYMKYISTQPMRLYCVTCEEVYYLPQNGSIKLYKEIICPL 660

Query: 662 DNFELLIFSLPGPEGKSFPLCPYCYNSPPFEGINTLYGAAKTNPSSGASSKGMGMPCFLC 721
           D FELL+FS+ GP+ KSFPLCP+CYNSPPFEGI+ L+GA K +  +G   KG GMPCFLC
Sbjct: 661 DGFELLLFSMVGPDAKSFPLCPFCYNSPPFEGIDKLFGALKLD-DTGKVGKGAGMPCFLC 720

Query: 722 PHPTCRHSLITQGVCACPECSGTLVLDPVSAPNWKLNCNKCNCLVFLPQGAHRISTTNEK 781
            HPTC+ S+ITQGVCACPEC+GTL+LDPVSAP W+L CN+CNC+V LP  AH+ISTT++K
Sbjct: 721 LHPTCKQSMITQGVCACPECTGTLILDPVSAPKWRLYCNRCNCIVLLPHAAHKISTTDKK 780

Query: 782 CADCDSTIIEVDFNKKTTPLEDGATLFVGCILCDEFLHSLLEMKHGKSFFRGRGSRGRGR 841
           C  C+STIIEVDFNKKTTPL+DGATL  GCILCDE LHSL+EMKHGKSFF  RG RGRGR
Sbjct: 781 CPTCESTIIEVDFNKKTTPLKDGATLHEGCILCDELLHSLIEMKHGKSFFMRRG-RGRGR 840

Query: 842 GRGRGRGGRGRGRNSGKLMDPKMSFRDF 870
           GRGRGRG     R S +  DPKMSFRDF
Sbjct: 841 GRGRGRGSSRGRRGSSRHDDPKMSFRDF 866

BLAST of Cla97C05G088880 vs. ExPASy Swiss-Prot
Match: Q9Z321 (DNA topoisomerase 3-beta-1 OS=Mus musculus OX=10090 GN=Top3b PE=1 SV=1)

HSP 1 Score: 915.2 bits (2364), Expect = 5.5e-265
Identity = 459/872 (52.64%), Postives = 591/872 (67.78%), Query Frame = 0

Query: 5   TVLMVAEKPSIALSIASALSHGQMSTRK---GSTDVHEFDGRFLGHHAHYKVTSVIGHVF 64
           TVLMVAEKPS+A SIA  LS G MS+ K   G+  VH++ G F G   H+K+TSV GHV 
Sbjct: 3   TVLMVAEKPSLAQSIAKILSRGNMSSHKGLNGACSVHKYTGTFAGQPVHFKMTSVCGHVM 62

Query: 65  SIDFPAGFQDWKTTDPLDLF-QASVIKTVSNPKAHIRQHLSREARGCDCLVLWLDCDREG 124
           ++DF   +  W   DP +LF QA   K  +NPK ++ + L  E RGCD +VLWLDCD+EG
Sbjct: 63  TLDFLGKYNKWDKVDPAELFSQAPTEKKEANPKLNMVKFLQVEGRGCDYVVLWLDCDKEG 122

Query: 125 ENICFEVIEC---TGFGGNEIRKKVYRARFSSVTEKDILKALDNLVQPNRNEALAVDARQ 184
           ENICFEV++         +   K V+RARFSS+T+ DI  A+  L +P+ NEAL+VDARQ
Sbjct: 123 ENICFEVLDAVLPVMNNAHNGEKTVFRARFSSITDTDICNAMTRLSEPDHNEALSVDARQ 182

Query: 185 EIDLKVGVAFTRFQTTFFQGKYGNLDSRLISYGPCQTPTLGFCVQRHLQITTFKPEKFWS 244
           E+DL++G AFTRFQT +FQGKYG+LDS LIS+GPCQTPTLGFCV+RH +I +FKPE +W 
Sbjct: 183 ELDLRIGCAFTRFQTKYFQGKYGDLDSSLISFGPCQTPTLGFCVERHDKIQSFKPETYWV 242

Query: 245 VCPYIMQNGYE-LNLDWERGKLFDCDVTAMFQKRVMEDGIAEVTEISEKQENKVRPSGLN 304
           +   +  +  E L LDW+R ++FD ++  MF      +  A V   S K++ K RP  LN
Sbjct: 243 LQAKVHTDKEESLLLDWDRVRVFDWEIAQMFLNMTKLEKEAWVEATSRKEKAKQRPLALN 302

Query: 305 TVNLLKVASSALGFGPQMAMQLAERLYTQGFISYPRTESTAYPSSFDFKGTLHSLVKNPE 364
           TV +L+VASSALG GPQ AMQ+AERLYTQG+ISYPRTE+T YP +FD KG+L     +P 
Sbjct: 303 TVEMLRVASSALGMGPQHAMQIAERLYTQGYISYPRTETTHYPENFDLKGSLRQQANHPY 362

Query: 365 WGDYVRMLLSDGYSKPRSGTDVGDHPPITPMGLATEDMLGRDAWRLYQYVCQHFIGSLSS 424
           W D V+ LL++G ++PR G D GDHPPITPM  ATE  LG DAWRLY+Y+ +HFI ++S 
Sbjct: 363 WADSVKQLLAEGINRPRKGHDAGDHPPITPMKSATEAELGGDAWRLYEYITRHFIATVSH 422

Query: 425 DCKYIRTKIQFSIAGEIFNCTGSRVMVKGFTSIMPWLAVN-EKKLPQFLKGEKIEVARVE 484
           DCKY+++ I F I  E F C G  V+  GFT IMPW +V  E+ LP   KG+   V  V+
Sbjct: 423 DCKYLQSTISFRIGPEHFTCMGKTVISPGFTEIMPWQSVPLEESLPTCQKGDTFTVGEVK 482

Query: 485 LYQGNTTPPDYLSESELISLMEKHGIGTDASIPVHINNIGERNYVQVQAGRRLVPTNLGI 544
           + +  T+PPDYL+E+ELI+LMEKHGIGTDASIPVHINNI +RNYV V++GRRL PTNLGI
Sbjct: 483 MLEKQTSPPDYLTEAELITLMEKHGIGTDASIPVHINNICQRNYVTVESGRRLKPTNLGI 542

Query: 545 TLIRGYQCIDPDLCLPDIRSFIEQQITLVAKGQADHSSVVQNVLQQFKQKYSYFVKKIDH 604
            L+ GY  ID +L LP IRS +E+Q+ L+A+G+AD+  V+ + L  FK+K+ YFV  I  
Sbjct: 543 VLVHGYYKIDAELVLPTIRSAVEKQLNLIAQGKADYHQVLGHTLDIFKRKFHYFVDSIAG 602

Query: 605 MDALFEAQFSPLADSGRALSKCGKCLRYMKFISSQPQRLYCGTCEEVYYLPQKGAIKLYK 664
           MD L E  FSPLA +G+ LS+CGKC R+MK+I ++P RL+C  C+E Y LPQ G IKLYK
Sbjct: 603 MDELMEVSFSPLAATGKPLSRCGKCHRFMKYIQAKPSRLHCSHCDETYTLPQNGTIKLYK 662

Query: 665 ELTCPLDNFELLIFSLPGPEGKSFPLCPYCYNSPPFEGINTLYGAAKTNPSSGASSKGMG 724
           EL CPLD+FEL+++S  G  GKS+PLCPYCYN PPF  +                 KGMG
Sbjct: 663 ELRCPLDDFELVLWS-SGSRGKSYPLCPYCYNHPPFRDM----------------KKGMG 722

Query: 725 MPCFLCPHPTCRHSLITQGVCACPEC-SGTLVLDPVSAPNWKLNCNKCNCLVFLPQGAHR 784
             C  C HPTC+HSL   G+  C EC +G LVLDP S P WK+ CN CN +    + AHR
Sbjct: 723 --CNECTHPTCQHSLSMLGIGQCVECENGVLVLDPTSGPKWKVACNTCNVVAHCFENAHR 782

Query: 785 ISTTNEKCADCDSTIIEVDFNKKTTPLEDGATLFVGCILCDEFLHSLLEMKHGKSFF-RG 844
           +  + + C  C++ +++VDFNK  +PL    T   GCI CD     L+E+KH  S     
Sbjct: 783 VRVSADTCNTCEAALLDVDFNKAKSPLPGNETQHTGCIFCDPVFQELVELKHAASCHPMH 842

Query: 845 RGSRGRGRGRGRGRGGRGRGRNSGKLMDPKMS 866
           RG  GR +GRGRGRG R  G+ + +    KMS
Sbjct: 843 RGGPGRRQGRGRGRGRRPPGKPNPRRPKDKMS 855

BLAST of Cla97C05G088880 vs. ExPASy Swiss-Prot
Match: O95985 (DNA topoisomerase 3-beta-1 OS=Homo sapiens OX=9606 GN=TOP3B PE=1 SV=1)

HSP 1 Score: 911.0 bits (2353), Expect = 1.0e-263
Identity = 455/875 (52.00%), Postives = 592/875 (67.66%), Query Frame = 0

Query: 5   TVLMVAEKPSIALSIASALSHGQMSTRK---GSTDVHEFDGRFLGHHAHYKVTSVIGHVF 64
           TVLMVAEKPS+A SIA  LS G +S+ K   G+  VHE+ G F G    +K+TSV GHV 
Sbjct: 3   TVLMVAEKPSLAQSIAKILSRGSLSSHKGLNGACSVHEYTGTFAGQPVRFKMTSVCGHVM 62

Query: 65  SIDFPAGFQDWKTTDPLDLF-QASVIKTVSNPKAHIRQHLSREARGCDCLVLWLDCDREG 124
           ++DF   +  W   DP +LF QA   K  +NPK ++ + L  E RGCD +VLWLDCD+EG
Sbjct: 63  TLDFLGKYNKWDKVDPAELFSQAPTEKKEANPKLNMVKFLQVEGRGCDYIVLWLDCDKEG 122

Query: 125 ENICFEVIECT------GFGGNEIRKKVYRARFSSVTEKDILKALDNLVQPNRNEALAVD 184
           ENICFEV++          GG    K V+RARFSS+T+ DI  A+  L +P+ NEAL+VD
Sbjct: 123 ENICFEVLDAVLPVMNKAHGG---EKTVFRARFSSITDTDICNAMACLGEPDHNEALSVD 182

Query: 185 ARQEIDLKVGVAFTRFQTTFFQGKYGNLDSRLISYGPCQTPTLGFCVQRHLQITTFKPEK 244
           ARQE+DL++G AFTRFQT +FQGKYG+LDS LIS+GPCQTPTLGFCV+RH +I +FKPE 
Sbjct: 183 ARQELDLRIGCAFTRFQTKYFQGKYGDLDSSLISFGPCQTPTLGFCVERHDKIQSFKPET 242

Query: 245 FWSVCPYI-MQNGYELNLDWERGKLFDCDVTAMFQKRVMEDGIAEVTEISEKQENKVRPS 304
           +W +   +       L LDW+R ++FD ++  MF      +  A+V   S K++ K RP 
Sbjct: 243 YWVLQAKVNTDKDRSLLLDWDRVRVFDREIAQMFLNMTKLEKEAQVEATSRKEKAKQRPL 302

Query: 305 GLNTVNLLKVASSALGFGPQMAMQLAERLYTQGFISYPRTESTAYPSSFDFKGTLHSLVK 364
            LNTV +L+VASS+LG GPQ AMQ AERLYTQG+ISYPRTE+T YP +FD KG+L     
Sbjct: 303 ALNTVEMLRVASSSLGMGPQHAMQTAERLYTQGYISYPRTETTHYPENFDLKGSLRQQAN 362

Query: 365 NPEWGDYVRMLLSDGYSKPRSGTDVGDHPPITPMGLATEDMLGRDAWRLYQYVCQHFIGS 424
           +P W D V+ LL++G ++PR G D GDHPPITPM  ATE  LG DAWRLY+Y+ +HFI +
Sbjct: 363 HPYWADTVKRLLAEGINRPRKGHDAGDHPPITPMKSATEAELGGDAWRLYEYITRHFIAT 422

Query: 425 LSSDCKYIRTKIQFSIAGEIFNCTGSRVMVKGFTSIMPWLAVN-EKKLPQFLKGEKIEVA 484
           +S DCKY+++ I F I  E+F C+G  V+  GFT +MPW +V  E+ LP   +G+   V 
Sbjct: 423 VSHDCKYLQSTISFRIGPELFTCSGKTVLSPGFTEVMPWQSVPLEESLPTCQRGDAFPVG 482

Query: 485 RVELYQGNTTPPDYLSESELISLMEKHGIGTDASIPVHINNIGERNYVQVQAGRRLVPTN 544
            V++ +  T PPDYL+E+ELI+LMEKHGIGTDASIPVHINNI +RNYV V++GRRL PTN
Sbjct: 483 EVKMLEKQTNPPDYLTEAELITLMEKHGIGTDASIPVHINNICQRNYVTVESGRRLKPTN 542

Query: 545 LGITLIRGYQCIDPDLCLPDIRSFIEQQITLVAKGQADHSSVVQNVLQQFKQKYSYFVKK 604
           LGI L+ GY  ID +L LP IRS +E+Q+ L+A+G+AD+  V+ + L  FK+K+ YFV  
Sbjct: 543 LGIVLVHGYYKIDAELVLPTIRSAVEKQLNLIAQGKADYRQVLGHTLDVFKRKFHYFVDS 602

Query: 605 IDHMDALFEAQFSPLADSGRALSKCGKCLRYMKFISSQPQRLYCGTCEEVYYLPQKGAIK 664
           I  MD L E  FSPLA +G+ LS+CGKC R+MK+I ++P RL+C  C+E Y LPQ G IK
Sbjct: 603 IAGMDELMEVSFSPLAATGKPLSRCGKCHRFMKYIQAKPSRLHCSHCDETYTLPQNGTIK 662

Query: 665 LYKELTCPLDNFELLIFSLPGPEGKSFPLCPYCYNSPPFEGINTLYGAAKTNPSSGASSK 724
           LYKEL CPLD+FEL+++S  G  GKS+PLCPYCYN PPF  +                 K
Sbjct: 663 LYKELRCPLDDFELVLWS-SGSRGKSYPLCPYCYNHPPFRDM----------------KK 722

Query: 725 GMGMPCFLCPHPTCRHSLITQGVCACPEC-SGTLVLDPVSAPNWKLNCNKCNCLVFLPQG 784
           GMG  C  C HP+C+HSL   G+  C EC SG LVLDP S P WK+ CNKCN +    + 
Sbjct: 723 GMG--CNECTHPSCQHSLSMLGIGQCVECESGVLVLDPTSGPKWKVACNKCNVVAHCFEN 782

Query: 785 AHRISTTNEKCADCDSTIIEVDFNKKTTPLEDGATLFVGCILCDEFLHSLLEMKHGKSFF 844
           AHR+  + + C+ C++ +++VDFNK  +PL    T  +GC+ CD     L+E+KH  S  
Sbjct: 783 AHRVRVSADTCSVCEAALLDVDFNKAKSPLPGDETQHMGCVFCDPVFQELVELKHAASCH 842

Query: 845 -RGRGSRGRGRGRGRGRGGRGRGRNSGKLMDPKMS 866
              RG  GR +GRGRGR  R  G+ + +    KMS
Sbjct: 843 PMHRGGPGRRQGRGRGRARRPPGKPNPRRPKDKMS 855

BLAST of Cla97C05G088880 vs. ExPASy Swiss-Prot
Match: O96651 (DNA topoisomerase 3-beta OS=Drosophila melanogaster OX=7227 GN=Top3beta PE=2 SV=2)

HSP 1 Score: 830.5 bits (2144), Expect = 1.8e-239
Identity = 416/857 (48.54%), Postives = 561/857 (65.46%), Query Frame = 0

Query: 5   TVLMVAEKPSIALSIASALSHGQMSTRKGS---TDVHEFDGRFLGH-HAHYKVTSVIGHV 64
           +VLMVAEKPS+A S+A  LS+G+ + ++G+      HE+ G F      H+++TSV GHV
Sbjct: 3   SVLMVAEKPSLAASLAGILSNGRCTAKRGTGNGCSTHEWTGNFRNEGSVHFRMTSVCGHV 62

Query: 65  FSIDFPAGFQDWKTTDPLDLFQASVIKTVSNPKAHIRQHLSREARGCDCLVLWLDCDREG 124
            S+DF   +  W   DP+ LF  +  K  +NPK ++R+ L+ EARGCD LVLWLDCD+EG
Sbjct: 63  MSLDFNKKYNCWDKVDPIQLFGCATEKKETNPKQNMRKFLAHEARGCDYLVLWLDCDKEG 122

Query: 125 ENICFEVIECTGFGGNEI--RKKVYRARFSSVTEKDILKALDNLVQPNRNEALAVDARQE 184
           ENICFEV++      N +   +  YRA FS++TEKDI KA++ L  PN NEA +VDARQE
Sbjct: 123 ENICFEVMDAVKHVINNVYSDQVTYRAHFSAITEKDIKKAMETLGHPNENEAKSVDARQE 182

Query: 185 IDLKVGVAFTRFQTTFFQGKYGNLDSRLISYGPCQTPTLGFCVQRHLQITTFKPEKFWSV 244
           +DL++G AFTRFQT FFQ +YG+LDS LISYGPCQTPTLGFCV+RH  I TFKPE FW +
Sbjct: 183 LDLRIGCAFTRFQTKFFQDRYGDLDSSLISYGPCQTPTLGFCVKRHDDIQTFKPESFWHL 242

Query: 245 CPYIMQNGYELNLDWERGKLFDCDVTAMFQKRVMEDGIAEVTEISEKQENKVRPSGLNTV 304
              ++    E+ L+W RG++F  D+  M   RV E   A V  ++ K+  K +P  LNTV
Sbjct: 243 --QLLAGQPEVTLEWARGRVFKKDIAIMLLNRVKEHKKATVESVASKEAYKSKPQALNTV 302

Query: 305 NLLKVASSALGFGPQMAMQLAERLYTQGFISYPRTESTAYPSSFDFKGTLHSLVKNPEWG 364
            L+++ SS LG GP  AMQ+AERLYTQG+ISYPRTE+  YP++FD    LH L  + ++G
Sbjct: 303 ELMRICSSGLGIGPFQAMQIAERLYTQGYISYPRTETNQYPTNFDLPAVLHVLKPSADFG 362

Query: 365 DYVRMLLSDGYSKPRSGTDVGDHPPITPMGLATEDMLGRDAWRLYQYVCQHFIGSLSSDC 424
           +  R +L D    PR G D GDHPPITPM L       RD WR+Y+++C+HF+G++S D 
Sbjct: 363 EEARSILGD-IQTPRKGKDAGDHPPITPMKLGNRSDFDRDTWRVYEFICRHFMGTVSRDL 422

Query: 425 KYIRTKIQFSIAGEIFNCTGSRVMVKGFTSIMPWLAV-NEKKLPQFLKGEKIEVARVELY 484
           KY  T  + S+  E F+CT S ++  GFT +M W A   ++  P F++G ++ +  V L 
Sbjct: 423 KYRVTTAKLSVGMETFSCTASVLIDAGFTKVMTWSAFGKDEPQPPFVQGTQVAINDVRLI 482

Query: 485 QGNTTPPDYLSESELISLMEKHGIGTDASIPVHINNIGERNYVQVQAGRRLVPTNLGITL 544
           +  T PPDYL+ESELI+LME+HGIGTDASIPVHINNI +RNYV ++ GR+L+PT LGI L
Sbjct: 483 ESQTGPPDYLTESELITLMEEHGIGTDASIPVHINNICQRNYVHIENGRKLMPTTLGIVL 542

Query: 545 IRGYQCIDPDLCLPDIRSFIEQQITLVAKGQADHSSVVQNVLQQFKQKYSYFVKKIDHMD 604
           + GYQ IDP+L LP +R+ +E+ +TL+A+G A+   V+++ ++ FK K+ YFVK ID MD
Sbjct: 543 VHGYQKIDPELVLPTMRTEVERMLTLIAQGSANFQDVLRHAIKIFKLKFMYFVKNIDSMD 602

Query: 605 ALFEAQFSPLADSGRALSKCGKCLRYMKFISSQPQRLYCGTCEEVYYLPQKGAIKLYKEL 664
           ALFE  FSPLA+SG+A S+CGKC RYMK+I ++P RL+C  C+E Y LP  G +K+Y+E 
Sbjct: 603 ALFEVSFSPLAESGKAHSRCGKCRRYMKYIQTKPARLHCSHCDETYALP-IGNVKVYREF 662

Query: 665 TCPLDNFELLIFSLPGPEGKSFPLCPYCYNSPPFEGINTLYGAAKTNPSSGASSKGMGMP 724
            CPLD+F+LL FS  G +G+S+P CPYCYN PPF  +  L G                  
Sbjct: 663 KCPLDDFDLLAFS-TGVKGRSYPFCPYCYNHPPFSDMPHLGG------------------ 722

Query: 725 CFLCPHPTCRHSLITQGVCACPEC-SGTLVLDPVSAPNWKLNCNKCNCLVFLPQGAHRIS 784
           C  C +  C HSL T G+ +C EC +G LVLD   AP WKL CN+C+ ++   +GA +I+
Sbjct: 723 CNTCTNANCPHSLNTLGISSCVECPTGVLVLDCTLAPTWKLGCNRCDVIINCFKGATKIT 782

Query: 785 TTNEKCADCDSTIIEVDFNKKTTPLEDGATLFVGCILCDEFLHSLLEMKHGKSFFRGRGS 844
               KC +C +  + V +    +  +DG+    GCI C      L+E KH     R   S
Sbjct: 783 VEEAKCQECGAQQVNVVYKSDKSKFKDGSEEKSGCIFCSADFSHLVE-KHRAVASRPVRS 835

Query: 845 RGRGRGRGRGRGGRGRG 854
            G  RG   GRGG G G
Sbjct: 843 GGGFRGGKAGRGGGGMG 835

BLAST of Cla97C05G088880 vs. ExPASy TrEMBL
Match: A0A6J1GWS0 (DNA topoisomerase OS=Cucurbita moschata OX=3662 GN=LOC111457562 PE=3 SV=1)

HSP 1 Score: 1735.3 bits (4493), Expect = 0.0e+00
Identity = 833/869 (95.86%), Postives = 854/869 (98.27%), Query Frame = 0

Query: 1   MVASTVLMVAEKPSIALSIASALSHGQMSTRKGSTDVHEFDGRFLGHHAHYKVTSVIGHV 60
           MVAS+VLMVAEKPSIALSIASALSHGQMSTRKGST+VHEFDGRFLGHHAHYKVTSVIGHV
Sbjct: 1   MVASSVLMVAEKPSIALSIASALSHGQMSTRKGSTEVHEFDGRFLGHHAHYKVTSVIGHV 60

Query: 61  FSIDFPAGFQDWKTTDPLDLFQASVIKTVSNPKAHIRQHLSREARGCDCLVLWLDCDREG 120
           FSIDFPA FQDWK TDPLDLFQASVIKT SNPKAHIRQHLSREARGCDCLVLWLDCDREG
Sbjct: 61  FSIDFPARFQDWKATDPLDLFQASVIKTESNPKAHIRQHLSREARGCDCLVLWLDCDREG 120

Query: 121 ENICFEVIECTGFGGNEIRKKVYRARFSSVTEKDILKALDNLVQPNRNEALAVDARQEID 180
           ENICFEVIECTGFGGNE RKKVYRARFSSVTEKDILKALDNLV+PNRNEALAVDARQEID
Sbjct: 121 ENICFEVIECTGFGGNEYRKKVYRARFSSVTEKDILKALDNLVEPNRNEALAVDARQEID 180

Query: 181 LKVGVAFTRFQTTFFQGKYGNLDSRLISYGPCQTPTLGFCVQRHLQITTFKPEKFWSVCP 240
           LKVGV+FTRFQTTFFQGKYGNLDSRLISYGPCQTPTLGFCVQRHLQITTFKPEKFWSVCP
Sbjct: 181 LKVGVSFTRFQTTFFQGKYGNLDSRLISYGPCQTPTLGFCVQRHLQITTFKPEKFWSVCP 240

Query: 241 YIMQNGYELNLDWERGKLFDCDVTAMFQKRVMEDGIAEVTEISEKQENKVRPSGLNTVNL 300
           YIMQNGYELNLDWERG+LFDCDV  MFQKRVMEDGIAEVT+ISEKQE+KVRPSGLNTVNL
Sbjct: 241 YIMQNGYELNLDWERGRLFDCDVATMFQKRVMEDGIAEVTQISEKQESKVRPSGLNTVNL 300

Query: 301 LKVASSALGFGPQMAMQLAERLYTQGFISYPRTESTAYPSSFDFKGTLHSLVKNPEWGDY 360
           LKVASSALG+GPQMAMQLAERLYTQGFISYPRTESTAYPSSFDFKGTLHSLVKNPEWGDY
Sbjct: 301 LKVASSALGYGPQMAMQLAERLYTQGFISYPRTESTAYPSSFDFKGTLHSLVKNPEWGDY 360

Query: 361 VRMLLSDGYSKPRSGTDVGDHPPITPMGLATEDMLGRDAWRLYQYVCQHFIGSLSSDCKY 420
           VR+LLSDGYSKPRSGTDVGDHPPITPM LATEDM+GRDAWRLYQYVCQHFIGSLSSDCKY
Sbjct: 361 VRVLLSDGYSKPRSGTDVGDHPPITPMSLATEDMVGRDAWRLYQYVCQHFIGSLSSDCKY 420

Query: 421 IRTKIQFSIAGEIFNCTGSRVMVKGFTSIMPWLAVNEKKLPQFLKGEKIEVARVELYQGN 480
           +RTKIQFSIAGEIFNCTGS VMVKGFTSIMPWLAVNEKKLPQFLKG+KIEVA+V+LYQGN
Sbjct: 421 LRTKIQFSIAGEIFNCTGSSVMVKGFTSIMPWLAVNEKKLPQFLKGQKIEVAKVDLYQGN 480

Query: 481 TTPPDYLSESELISLMEKHGIGTDASIPVHINNIGERNYVQVQAGRRLVPTNLGITLIRG 540
           T+PPDYLSESELISLMEKHGIGTDASIPVHINNIGERNYVQVQAGRRLVPTNLGITLIRG
Sbjct: 481 TSPPDYLSESELISLMEKHGIGTDASIPVHINNIGERNYVQVQAGRRLVPTNLGITLIRG 540

Query: 541 YQCIDPDLCLPDIRSFIEQQITLVAKGQADHSSVVQNVLQQFKQKYSYFVKKIDHMDALF 600
           YQCIDPDLCLPDIRSFIEQQIT VAKGQADH SVVQNVLQQFKQKYSYFVKKIDHMDALF
Sbjct: 541 YQCIDPDLCLPDIRSFIEQQITFVAKGQADHFSVVQNVLQQFKQKYSYFVKKIDHMDALF 600

Query: 601 EAQFSPLADSGRALSKCGKCLRYMKFISSQPQRLYCGTCEEVYYLPQKGAIKLYKELTCP 660
           EAQFSPLA+SGRALSKCGKCLRYMKFISSQPQRLYCGTCEEVYYLPQ+GAIKLYKELTCP
Sbjct: 601 EAQFSPLAESGRALSKCGKCLRYMKFISSQPQRLYCGTCEEVYYLPQRGAIKLYKELTCP 660

Query: 661 LDNFELLIFSLPGPEGKSFPLCPYCYNSPPFEGINTLYGAAKTNPSSGASSKGMGMPCFL 720
           LDNFELLIFSLPGPEGKSFPLCPYCYNSPPFEGI+TLYGAAKTNPSSGASSKGMGMPCFL
Sbjct: 661 LDNFELLIFSLPGPEGKSFPLCPYCYNSPPFEGIDTLYGAAKTNPSSGASSKGMGMPCFL 720

Query: 721 CPHPTCRHSLITQGVCACPECSGTLVLDPVSAPNWKLNCNKCNCLVFLPQGAHRISTTNE 780
           CPHPTCRHSLITQGVCACPECSGTLVLDPV APNWKLNCNKCNCLVFLPQGAHRI+TTNE
Sbjct: 721 CPHPTCRHSLITQGVCACPECSGTLVLDPVGAPNWKLNCNKCNCLVFLPQGAHRIATTNE 780

Query: 781 KCADCDSTIIEVDFNKKTTPLEDGATLFVGCILCDEFLHSLLEMKHGKSFFRGRGSRGRG 840
           KCA+CDSTIIEVDFNKKTTPLE GATLFVGCILCDEFLHSLLEMKHGKSFFRGRGSRGRG
Sbjct: 781 KCAECDSTIIEVDFNKKTTPLEGGATLFVGCILCDEFLHSLLEMKHGKSFFRGRGSRGRG 840

Query: 841 RGRGRGRGGRGRGRNSGKLMDPKMSFRDF 870
           RGRGR   GRGRGR+SGKLMDPKMSFRDF
Sbjct: 841 RGRGR---GRGRGRSSGKLMDPKMSFRDF 866

BLAST of Cla97C05G088880 vs. ExPASy TrEMBL
Match: A0A6J1IRJ0 (DNA topoisomerase OS=Cucurbita maxima OX=3661 GN=LOC111479340 PE=3 SV=1)

HSP 1 Score: 1733.8 bits (4489), Expect = 0.0e+00
Identity = 833/869 (95.86%), Postives = 853/869 (98.16%), Query Frame = 0

Query: 1   MVASTVLMVAEKPSIALSIASALSHGQMSTRKGSTDVHEFDGRFLGHHAHYKVTSVIGHV 60
           MVAS+VLMVAEKPSIALSIASALSHGQMSTRKGST+VHEFDGRFLGHHAHYKVTSVIGHV
Sbjct: 1   MVASSVLMVAEKPSIALSIASALSHGQMSTRKGSTEVHEFDGRFLGHHAHYKVTSVIGHV 60

Query: 61  FSIDFPAGFQDWKTTDPLDLFQASVIKTVSNPKAHIRQHLSREARGCDCLVLWLDCDREG 120
           FSIDFPA FQDWK TDPLDLFQASVIKT SNPKAHIRQHLSREARGCDCLVLWLDCDREG
Sbjct: 61  FSIDFPARFQDWKATDPLDLFQASVIKTESNPKAHIRQHLSREARGCDCLVLWLDCDREG 120

Query: 121 ENICFEVIECTGFGGNEIRKKVYRARFSSVTEKDILKALDNLVQPNRNEALAVDARQEID 180
           ENICFEVIECTGFGGNE RKKVYRARFSSVTEKDILKALDNLV+PNRNEALAVDARQEID
Sbjct: 121 ENICFEVIECTGFGGNENRKKVYRARFSSVTEKDILKALDNLVEPNRNEALAVDARQEID 180

Query: 181 LKVGVAFTRFQTTFFQGKYGNLDSRLISYGPCQTPTLGFCVQRHLQITTFKPEKFWSVCP 240
           LKVGV+FTRFQTTFFQGKYGNLDSRLISYGPCQTPTLGFCVQRHLQITTFKPEKFWSVCP
Sbjct: 181 LKVGVSFTRFQTTFFQGKYGNLDSRLISYGPCQTPTLGFCVQRHLQITTFKPEKFWSVCP 240

Query: 241 YIMQNGYELNLDWERGKLFDCDVTAMFQKRVMEDGIAEVTEISEKQENKVRPSGLNTVNL 300
           YIMQNGYELNLDWERG+LFDCDV  MFQKRVMEDGIAEVT+ISEKQE+KVRPSGLNTVNL
Sbjct: 241 YIMQNGYELNLDWERGRLFDCDVATMFQKRVMEDGIAEVTQISEKQESKVRPSGLNTVNL 300

Query: 301 LKVASSALGFGPQMAMQLAERLYTQGFISYPRTESTAYPSSFDFKGTLHSLVKNPEWGDY 360
           LKVASSALG+GPQMAMQLAERLYTQGFISYPRTESTAYPSSFDFKGTLHSLVKNPEWGDY
Sbjct: 301 LKVASSALGYGPQMAMQLAERLYTQGFISYPRTESTAYPSSFDFKGTLHSLVKNPEWGDY 360

Query: 361 VRMLLSDGYSKPRSGTDVGDHPPITPMGLATEDMLGRDAWRLYQYVCQHFIGSLSSDCKY 420
           VRMLLSDGYSKPRSGTDVGDHPPITPM LATEDM+GRDAWRLYQYVCQHFIGSLSSDCKY
Sbjct: 361 VRMLLSDGYSKPRSGTDVGDHPPITPMSLATEDMVGRDAWRLYQYVCQHFIGSLSSDCKY 420

Query: 421 IRTKIQFSIAGEIFNCTGSRVMVKGFTSIMPWLAVNEKKLPQFLKGEKIEVARVELYQGN 480
           IRTKIQFSIAGEIFNCTGS V+VKGFTSIMPWLAVNEKKLPQFLKG+KIEVA+V+LYQGN
Sbjct: 421 IRTKIQFSIAGEIFNCTGSSVLVKGFTSIMPWLAVNEKKLPQFLKGQKIEVAKVDLYQGN 480

Query: 481 TTPPDYLSESELISLMEKHGIGTDASIPVHINNIGERNYVQVQAGRRLVPTNLGITLIRG 540
           T+PPDYLSESELISLMEKHGIGTDASIPVHINNIGERNYVQVQAGRRLVPTNLGITLIRG
Sbjct: 481 TSPPDYLSESELISLMEKHGIGTDASIPVHINNIGERNYVQVQAGRRLVPTNLGITLIRG 540

Query: 541 YQCIDPDLCLPDIRSFIEQQITLVAKGQADHSSVVQNVLQQFKQKYSYFVKKIDHMDALF 600
           YQCIDPDLCLPDIRSFIEQQIT VAKGQADH SVVQNVLQQFKQKYSYFVKKIDHMDALF
Sbjct: 541 YQCIDPDLCLPDIRSFIEQQITFVAKGQADHFSVVQNVLQQFKQKYSYFVKKIDHMDALF 600

Query: 601 EAQFSPLADSGRALSKCGKCLRYMKFISSQPQRLYCGTCEEVYYLPQKGAIKLYKELTCP 660
           EAQFSPLA+SGRALSKCGKCLRYMKFISSQPQRLYCGTCEEVYYLPQ+GAIKLYKELTCP
Sbjct: 601 EAQFSPLAESGRALSKCGKCLRYMKFISSQPQRLYCGTCEEVYYLPQRGAIKLYKELTCP 660

Query: 661 LDNFELLIFSLPGPEGKSFPLCPYCYNSPPFEGINTLYGAAKTNPSSGASSKGMGMPCFL 720
           LDNFELLIFSLPGPEGKSFPLCPYCYNSPPFEGI+ LYGAAKTNPSSGASSKGMGMPCFL
Sbjct: 661 LDNFELLIFSLPGPEGKSFPLCPYCYNSPPFEGIDALYGAAKTNPSSGASSKGMGMPCFL 720

Query: 721 CPHPTCRHSLITQGVCACPECSGTLVLDPVSAPNWKLNCNKCNCLVFLPQGAHRISTTNE 780
           CPHPTCRHSLITQGVCACPECSGTLVLDPV APNWKLNCNKCNCLVFLPQGAHRI+TTNE
Sbjct: 721 CPHPTCRHSLITQGVCACPECSGTLVLDPVGAPNWKLNCNKCNCLVFLPQGAHRIATTNE 780

Query: 781 KCADCDSTIIEVDFNKKTTPLEDGATLFVGCILCDEFLHSLLEMKHGKSFFRGRGSRGRG 840
           KCA+CDSTIIEVDFNKKTTPLE GATLFVGCILCDEFLHSLLEMKHGKSFFRGRGSRGRG
Sbjct: 781 KCAECDSTIIEVDFNKKTTPLEGGATLFVGCILCDEFLHSLLEMKHGKSFFRGRGSRGRG 840

Query: 841 RGRGRGRGGRGRGRNSGKLMDPKMSFRDF 870
           RGRGR   GRGRGR+SGKLMDPKMSFRDF
Sbjct: 841 RGRGR---GRGRGRSSGKLMDPKMSFRDF 866

BLAST of Cla97C05G088880 vs. ExPASy TrEMBL
Match: A0A1S3AYH7 (DNA topoisomerase OS=Cucumis melo OX=3656 GN=LOC103483959 PE=3 SV=1)

HSP 1 Score: 1729.5 bits (4478), Expect = 0.0e+00
Identity = 835/869 (96.09%), Postives = 849/869 (97.70%), Query Frame = 0

Query: 1   MVASTVLMVAEKPSIALSIASALSHGQMSTRKGSTDVHEFDGRFLGHHAHYKVTSVIGHV 60
           MVASTVLMVAEKPSIALSIASALSHGQMSTRKGST+VHEFDGRFLGHHA+YKVTSVIGH+
Sbjct: 1   MVASTVLMVAEKPSIALSIASALSHGQMSTRKGSTEVHEFDGRFLGHHAYYKVTSVIGHI 60

Query: 61  FSIDFPAGFQDWKTTDPLDLFQASVIKTVSNPKAHIRQHLSREARGCDCLVLWLDCDREG 120
           FSIDF AGFQDWKTT+PLDLFQASVIKT SNPKAHIRQHLSREARGCDCLVLWLDCDREG
Sbjct: 61  FSIDFQAGFQDWKTTNPLDLFQASVIKTESNPKAHIRQHLSREARGCDCLVLWLDCDREG 120

Query: 121 ENICFEVIECTGFGGNEIRKKVYRARFSSVTEKDILKALDNLVQPNRNEALAVDARQEID 180
           ENICFEVIECTGFGGN  RKKVYRARFSSVTEKDILKALDNLV+PNRNEALAVDARQEID
Sbjct: 121 ENICFEVIECTGFGGNGTRKKVYRARFSSVTEKDILKALDNLVEPNRNEALAVDARQEID 180

Query: 181 LKVGVAFTRFQTTFFQGKYGNLDSRLISYGPCQTPTLGFCVQRHLQITTFKPEKFWSVCP 240
           LKVGVAFTRFQTTFFQGKYGNLDSRLISYGPCQTPTLGFCVQRHLQITTFKPEKFWSVCP
Sbjct: 181 LKVGVAFTRFQTTFFQGKYGNLDSRLISYGPCQTPTLGFCVQRHLQITTFKPEKFWSVCP 240

Query: 241 YIMQNGYELNLDWERGKLFDCDVTAMFQKRVMEDGIAEVTEISEKQENKVRPSGLNTVNL 300
           YIMQNGYELNLDWERGKLFDCDVTAMFQKRV+EDGIAEVTEISEKQENKVRPSGLNTVNL
Sbjct: 241 YIMQNGYELNLDWERGKLFDCDVTAMFQKRVIEDGIAEVTEISEKQENKVRPSGLNTVNL 300

Query: 301 LKVASSALGFGPQMAMQLAERLYTQGFISYPRTESTAYPSSFDFKGTLHSLVKNPEWGDY 360
           LKVASSALGFGPQMAMQLAERLYTQGFISYPRTESTAYPSSFDFKGTLH+LVKNPEWGDY
Sbjct: 301 LKVASSALGFGPQMAMQLAERLYTQGFISYPRTESTAYPSSFDFKGTLHTLVKNPEWGDY 360

Query: 361 VRMLLSDGYSKPRSGTDVGDHPPITPMGLATEDMLGRDAWRLYQYVCQHFIGSLSSDCKY 420
           VRMLLSDGYSKPRSGTDVGDHPPITPM  ATEDMLGRDAWRLYQYVCQHFIGSLSSDCKY
Sbjct: 361 VRMLLSDGYSKPRSGTDVGDHPPITPMCSATEDMLGRDAWRLYQYVCQHFIGSLSSDCKY 420

Query: 421 IRTKIQFSIAGEIFNCTGSRVMVKGFTSIMPWLAVNEKKLPQFLKGEKIEVARVELYQGN 480
           IRTKIQFSIAGEIFNCTGSRV VKGFTSIMPWLAVNEKKLPQFLKGEKIEVARV+LYQGN
Sbjct: 421 IRTKIQFSIAGEIFNCTGSRVTVKGFTSIMPWLAVNEKKLPQFLKGEKIEVARVDLYQGN 480

Query: 481 TTPPDYLSESELISLMEKHGIGTDASIPVHINNIGERNYVQVQAGRRLVPTNLGITLIRG 540
           TTPPDYLSESELISLMEKHGIGTDASIPVHINNIGERNYVQVQAGRRLVPTNLGITLIRG
Sbjct: 481 TTPPDYLSESELISLMEKHGIGTDASIPVHINNIGERNYVQVQAGRRLVPTNLGITLIRG 540

Query: 541 YQCIDPDLCLPDIRSFIEQQITLVAKGQADHSSVVQNVLQQFKQKYSYFVKKIDHMDALF 600
           YQCIDPDLCLPDIRSFIE QITLVAKGQADHSSVV++VLQQFKQKYSYFVKKID MDALF
Sbjct: 541 YQCIDPDLCLPDIRSFIEHQITLVAKGQADHSSVVESVLQQFKQKYSYFVKKIDRMDALF 600

Query: 601 EAQFSPLADSGRALSKCGKCLRYMKFISSQPQRLYCGTCEEVYYLPQKGAIKLYKELTCP 660
           EAQFSPLADSGRALSKCGKCLRYMKFISSQPQRLYCG CEEVYYLPQKG IKLYKELTCP
Sbjct: 601 EAQFSPLADSGRALSKCGKCLRYMKFISSQPQRLYCGICEEVYYLPQKGTIKLYKELTCP 660

Query: 661 LDNFELLIFSLPGPEGKSFPLCPYCYNSPPFEGINTLYGAAKTNPSSGASSKGMGMPCFL 720
           LDNFELLI SLPGPEGKSFPLCPYCYNSPPFEGI+TLYGA+KTNPSSG SSKGMGMPCFL
Sbjct: 661 LDNFELLILSLPGPEGKSFPLCPYCYNSPPFEGIDTLYGASKTNPSSGTSSKGMGMPCFL 720

Query: 721 CPHPTCRHSLITQGVCACPECSGTLVLDPVSAPNWKLNCNKCNCLVFLPQGAHRISTTNE 780
           CPHPTCRHSLITQGVCACPECSGTLVLDPVSAPNWKLNCNKCNCLVFLPQGAHRISTT++
Sbjct: 721 CPHPTCRHSLITQGVCACPECSGTLVLDPVSAPNWKLNCNKCNCLVFLPQGAHRISTTSD 780

Query: 781 KCADCDSTIIEVDFNKKTTPLEDGATLFVGCILCDEFLHSLLEMKHGKSFFRGRGSRGRG 840
           KCADCDSTIIEVDFNKKTTPLEDGATLFVGCILCDEFLHSLLEMKHG SF R R SRGRG
Sbjct: 781 KCADCDSTIIEVDFNKKTTPLEDGATLFVGCILCDEFLHSLLEMKHGNSFIRNRFSRGRG 840

Query: 841 RGRGRGRGGRGRGRNSGKLMDPKMSFRDF 870
           RGRGRGRG  GRGRNSGK MDPKMSFRDF
Sbjct: 841 RGRGRGRGRGGRGRNSGKPMDPKMSFRDF 869

BLAST of Cla97C05G088880 vs. ExPASy TrEMBL
Match: A0A0A0L9C7 (DNA topoisomerase OS=Cucumis sativus OX=3659 GN=Csa_3G172970 PE=3 SV=1)

HSP 1 Score: 1726.8 bits (4471), Expect = 0.0e+00
Identity = 833/869 (95.86%), Postives = 849/869 (97.70%), Query Frame = 0

Query: 1   MVASTVLMVAEKPSIALSIASALSHGQMSTRKGSTDVHEFDGRFLGHHAHYKVTSVIGHV 60
           MVA TVLMVAEKPSIALSIASALSHGQMSTRKGST+VHEFDGRFLGHHA+YKVTSVIGHV
Sbjct: 1   MVAPTVLMVAEKPSIALSIASALSHGQMSTRKGSTEVHEFDGRFLGHHAYYKVTSVIGHV 60

Query: 61  FSIDFPAGFQDWKTTDPLDLFQASVIKTVSNPKAHIRQHLSREARGCDCLVLWLDCDREG 120
           FSIDF AGFQDWK+TDPLDLFQASVIKT SNPKAHI QHLSREARGCDCLVLWLDCDREG
Sbjct: 61  FSIDFQAGFQDWKSTDPLDLFQASVIKTESNPKAHICQHLSREARGCDCLVLWLDCDREG 120

Query: 121 ENICFEVIECTGFGGNEIRKKVYRARFSSVTEKDILKALDNLVQPNRNEALAVDARQEID 180
           ENICFEVIECTGFGGN  RKKVYRARFSSVTEKDILKALDNLV+PNRNEALAVDARQEID
Sbjct: 121 ENICFEVIECTGFGGNGTRKKVYRARFSSVTEKDILKALDNLVEPNRNEALAVDARQEID 180

Query: 181 LKVGVAFTRFQTTFFQGKYGNLDSRLISYGPCQTPTLGFCVQRHLQITTFKPEKFWSVCP 240
           LKVGVAFTRFQTTFFQGKYGNLDSRLISYGPCQTPTLGFCVQRHLQITTFKPEKFWSVCP
Sbjct: 181 LKVGVAFTRFQTTFFQGKYGNLDSRLISYGPCQTPTLGFCVQRHLQITTFKPEKFWSVCP 240

Query: 241 YIMQNGYELNLDWERGKLFDCDVTAMFQKRVMEDGIAEVTEISEKQENKVRPSGLNTVNL 300
           YI+Q GYELNLDWERGKLFDCDVTAMFQKRV+EDGIAEVTEISEKQENKVRPSGLNTVNL
Sbjct: 241 YILQTGYELNLDWERGKLFDCDVTAMFQKRVIEDGIAEVTEISEKQENKVRPSGLNTVNL 300

Query: 301 LKVASSALGFGPQMAMQLAERLYTQGFISYPRTESTAYPSSFDFKGTLHSLVKNPEWGDY 360
           LKVASSALGFGPQMAMQLAERLYTQGFISYPRTESTAYPSSFDFKGTLH+LVKNPEWGDY
Sbjct: 301 LKVASSALGFGPQMAMQLAERLYTQGFISYPRTESTAYPSSFDFKGTLHTLVKNPEWGDY 360

Query: 361 VRMLLSDGYSKPRSGTDVGDHPPITPMGLATEDMLGRDAWRLYQYVCQHFIGSLSSDCKY 420
           VRMLLSDGYSKPRSGTDVGDHPPITPM LATEDMLGRDAWRLYQYVCQHFIGSLSSDCKY
Sbjct: 361 VRMLLSDGYSKPRSGTDVGDHPPITPMCLATEDMLGRDAWRLYQYVCQHFIGSLSSDCKY 420

Query: 421 IRTKIQFSIAGEIFNCTGSRVMVKGFTSIMPWLAVNEKKLPQFLKGEKIEVARVELYQGN 480
           IRTKIQFSIAGEIFNCTGSRV VKGFTSIMPWLAVNEKKLPQFLKGEKIEVARV+LYQGN
Sbjct: 421 IRTKIQFSIAGEIFNCTGSRVTVKGFTSIMPWLAVNEKKLPQFLKGEKIEVARVDLYQGN 480

Query: 481 TTPPDYLSESELISLMEKHGIGTDASIPVHINNIGERNYVQVQAGRRLVPTNLGITLIRG 540
           TTPPDYLSESELISLMEKHGIGTDASIPVHINNIGERNYVQVQAGRRLVPTNLGITLIRG
Sbjct: 481 TTPPDYLSESELISLMEKHGIGTDASIPVHINNIGERNYVQVQAGRRLVPTNLGITLIRG 540

Query: 541 YQCIDPDLCLPDIRSFIEQQITLVAKGQADHSSVVQNVLQQFKQKYSYFVKKIDHMDALF 600
           YQCIDPDLCLPDIRSFIE QITLVAKGQADHSSVVQ+VLQQF+QKYSYFVKKIDHMDALF
Sbjct: 541 YQCIDPDLCLPDIRSFIEHQITLVAKGQADHSSVVQSVLQQFRQKYSYFVKKIDHMDALF 600

Query: 601 EAQFSPLADSGRALSKCGKCLRYMKFISSQPQRLYCGTCEEVYYLPQKGAIKLYKELTCP 660
           EAQFSPLADSGRALSKCGKCLRYMKFISSQPQRLYCG CEEVYYLPQKG IKLYKELTCP
Sbjct: 601 EAQFSPLADSGRALSKCGKCLRYMKFISSQPQRLYCGICEEVYYLPQKGTIKLYKELTCP 660

Query: 661 LDNFELLIFSLPGPEGKSFPLCPYCYNSPPFEGINTLYGAAKTNPSSGASSKGMGMPCFL 720
           LDNFELLI+SLPGPEGKSFPLCPYCYNSPPFEGINTLYGA+K NPSSG SSKGMGMPCF+
Sbjct: 661 LDNFELLIYSLPGPEGKSFPLCPYCYNSPPFEGINTLYGASKANPSSGTSSKGMGMPCFM 720

Query: 721 CPHPTCRHSLITQGVCACPECSGTLVLDPVSAPNWKLNCNKCNCLVFLPQGAHRISTTNE 780
           CPHPTCRHSLITQGVCACPECSGTLVLDPVSAPNWKLNCNKCNCLVFLP+GAHRISTT +
Sbjct: 721 CPHPTCRHSLITQGVCACPECSGTLVLDPVSAPNWKLNCNKCNCLVFLPKGAHRISTTRD 780

Query: 781 KCADCDSTIIEVDFNKKTTPLEDGATLFVGCILCDEFLHSLLEMKHGKSFFRGRGSRGRG 840
           KCADCDSTIIEVDFNKKTTPLEDGATLFVGCILCDEFLHSLLEMKHGKSF R RGSRGRG
Sbjct: 781 KCADCDSTIIEVDFNKKTTPLEDGATLFVGCILCDEFLHSLLEMKHGKSFIRNRGSRGRG 840

Query: 841 RGRGRGRGGRGRGRNSGKLMDPKMSFRDF 870
           RGRGRGRG  GRGR+SGK MDPKMSFRDF
Sbjct: 841 RGRGRGRG--GRGRSSGKPMDPKMSFRDF 867

BLAST of Cla97C05G088880 vs. ExPASy TrEMBL
Match: A0A5A7VKD3 (DNA topoisomerase OS=Cucumis melo var. makuwa OX=1194695 GN=E6C27_scaffold70G00170 PE=3 SV=1)

HSP 1 Score: 1726.1 bits (4469), Expect = 0.0e+00
Identity = 837/871 (96.10%), Postives = 851/871 (97.70%), Query Frame = 0

Query: 1   MVASTVLMVAEKPSIALSIASALSHGQMSTRKGSTDVHEFDGRFLGHHAHYKVTSVIGHV 60
           MVASTVLMVAEKPSIALSIASALSHGQMSTRKGST+VHEFDGRFLGHHA+YKVTSVIGH+
Sbjct: 1   MVASTVLMVAEKPSIALSIASALSHGQMSTRKGSTEVHEFDGRFLGHHAYYKVTSVIGHI 60

Query: 61  FSIDFPAGFQDWKTTDPLDLFQASVIKTVSNPKAHIRQHLSREARGCDCLVLWLDCDREG 120
           FSIDF AGFQDWKTT+PLDLFQASVIKT SNPKAHIRQHLSREARGCDCLVLWLDCDREG
Sbjct: 61  FSIDFQAGFQDWKTTNPLDLFQASVIKTESNPKAHIRQHLSREARGCDCLVLWLDCDREG 120

Query: 121 ENICFEVIECTGFGGNEIRKKVYRARFSSVTEKDILKALDNLVQPNRNEALAVDARQEID 180
           ENICFEVIECTGFGGN  RKKVYRARFSSVTEKDILKALDNLV+PNRNEALAVDARQEID
Sbjct: 121 ENICFEVIECTGFGGNGTRKKVYRARFSSVTEKDILKALDNLVEPNRNEALAVDARQEID 180

Query: 181 LKVGVAFTRFQTTFFQGKYGNLDSRLISYGPCQTPTLGFCVQRHLQITTFKPEKFWSVCP 240
           LKVGVAFTRFQTTFFQGKYGNLDSRLISYGPCQTPTLGFCVQRHLQITTFKPEKFWSVCP
Sbjct: 181 LKVGVAFTRFQTTFFQGKYGNLDSRLISYGPCQTPTLGFCVQRHLQITTFKPEKFWSVCP 240

Query: 241 YIMQNGYELNLDWERGKLFDCDVTAMFQKRVMEDGIAEVTEISEKQENKVRPSGLNTVNL 300
           YIMQNGYELNLDWERGKLFDCDVTAMFQKRV+EDGIAEVTEISEKQENKVRPSGLNTVNL
Sbjct: 241 YIMQNGYELNLDWERGKLFDCDVTAMFQKRVIEDGIAEVTEISEKQENKVRPSGLNTVNL 300

Query: 301 LKVASSALGFGPQMAMQLAERLYTQGFISYPRTESTAYPSSFDFKGTLHSLVKNPEWGDY 360
           LKVASSALGFGPQMAMQLAERLYTQGFISYPRTESTAYPSSFDFKGTLH+LVKNPEWGDY
Sbjct: 301 LKVASSALGFGPQMAMQLAERLYTQGFISYPRTESTAYPSSFDFKGTLHTLVKNPEWGDY 360

Query: 361 VRMLLSDGYSKPRSGTDVGDHPPITPMGLATEDMLGRDAWRLYQYVCQHFIGSLSSDCKY 420
           VRMLLSDGYSKPRSGTDVGDHPPITPM  ATEDMLGRDAWRLYQYVCQHFIGSLSSDCKY
Sbjct: 361 VRMLLSDGYSKPRSGTDVGDHPPITPMCSATEDMLGRDAWRLYQYVCQHFIGSLSSDCKY 420

Query: 421 IRTKIQFSIAGEIFNCTGSRVMVKGFTSIMPWLAVNEKKLPQFLKGEKIEVARVELYQGN 480
           IRTKIQFSIAGEIFNCTGSRV VKGFTSIMPWLAVNEKKLPQFLKGEKIEVARV+LYQGN
Sbjct: 421 IRTKIQFSIAGEIFNCTGSRVTVKGFTSIMPWLAVNEKKLPQFLKGEKIEVARVDLYQGN 480

Query: 481 TTPPDYLSESELISLMEKHGIGTDASIPVHINNIGERNYVQVQAGRRLVPTNLGITLIRG 540
           TTPPDYLSESELISLMEKHGIGTDASIPVHINNIGERNYVQVQAGRRLVPTNLGITLIRG
Sbjct: 481 TTPPDYLSESELISLMEKHGIGTDASIPVHINNIGERNYVQVQAGRRLVPTNLGITLIRG 540

Query: 541 YQCIDPDLCLPDIRSFIEQQITLVAKGQADHSSVVQNVLQQFKQKYSYFVKKIDHMDALF 600
           YQCIDPDLCLPDIRSFIE QITLVAKGQADHSSVV++VLQQFKQKYSYFVKKID MDALF
Sbjct: 541 YQCIDPDLCLPDIRSFIEHQITLVAKGQADHSSVVESVLQQFKQKYSYFVKKIDRMDALF 600

Query: 601 EAQFSPLADSGRALSKCGKCLRYMKFISSQPQRLYCGTCEEVYYLPQKGAIKLYKELTCP 660
           EAQFSPLADSGRALSKCGKCLRYMKFISSQPQRLYCG CEEVYYLPQKG IKLYKELTCP
Sbjct: 601 EAQFSPLADSGRALSKCGKCLRYMKFISSQPQRLYCGICEEVYYLPQKGTIKLYKELTCP 660

Query: 661 LDNFELLIFSLPGPEGKSFPLCPYCYNSPPFEGINTLYGAAKTNPSSGASSKGMGMPCFL 720
           LDNFELLI SLPGPEGKSFPLCPYCYNSPPFEGI+TLYGA+KTNPSSG SSKGMGMPCFL
Sbjct: 661 LDNFELLILSLPGPEGKSFPLCPYCYNSPPFEGIDTLYGASKTNPSSGTSSKGMGMPCFL 720

Query: 721 CPHPTCRHSLITQGVCACPECSGTLVLDPVSAPNWKLNCNKCNCLVFLPQGAHRISTTNE 780
           CPHPTCRHSLITQGVCACPECSGTLVLDPVSAPNWKLNCNKCNCLVFLPQGAHRISTT++
Sbjct: 721 CPHPTCRHSLITQGVCACPECSGTLVLDPVSAPNWKLNCNKCNCLVFLPQGAHRISTTSD 780

Query: 781 KCADCDSTIIEVDFNKKTTPLEDGATLFVGCILCDEFLHSLLEMKHGKSFFRGRGSRGRG 840
           KCADCDSTIIEVDFNKKTTPLEDGATLFVGCILCDEFLHSLLEMKHG SF R R SRGRG
Sbjct: 781 KCADCDSTIIEVDFNKKTTPLEDGATLFVGCILCDEFLHSLLEMKHGNSFIRNRFSRGRG 840

Query: 841 RGRGRGRG-GR-GRGRNSGKLMDPKMSFRDF 870
           RGRGRGRG GR GRGRNSGK MDPKMSFRDF
Sbjct: 841 RGRGRGRGRGRGGRGRNSGKPMDPKMSFRDF 871

BLAST of Cla97C05G088880 vs. TAIR 10
Match: AT2G32000.1 (DNA topoisomerase, type IA, core )

HSP 1 Score: 1400.2 bits (3623), Expect = 0.0e+00
Identity = 662/864 (76.62%), Postives = 752/864 (87.04%), Query Frame = 0

Query: 6   VLMVAEKPSIALSIASALSHGQMSTRKGSTDVHEFDGRFLGHHAHYKVTSVIGHVFSIDF 65
           VLMVAEKPSIALSIAS LSHGQMSTR+GST+VHEFDG F G  AHY+VTSVIGHVFS+DF
Sbjct: 7   VLMVAEKPSIALSIASVLSHGQMSTRRGSTEVHEFDGMFRGFKAHYRVTSVIGHVFSVDF 66

Query: 66  PAGFQDWKTTDPLDLFQASVIKTVSNPKAHIRQHLSREARGCDCLVLWLDCDREGENICF 125
           P  +Q+W T DP DLF A +IK  SNPKAHI +HLS EARGC  +VLWLDCDREGENICF
Sbjct: 67  PEKYQNWATIDPQDLFDAPIIKKESNPKAHICRHLSNEARGCSYMVLWLDCDREGENICF 126

Query: 126 EVIECTGFGGNEIRKKVYRARFSSVTEKDILKALDNLVQPNRNEALAVDARQEIDLKVGV 185
           EVIE TGF   + ++KVYRARFSSVTEKDI KA+DNLV+PNR+EALAVDARQEIDLKVGV
Sbjct: 127 EVIESTGFDMKDSKRKVYRARFSSVTEKDISKAMDNLVEPNRDEALAVDARQEIDLKVGV 186

Query: 186 AFTRFQTTFFQGKYGNLDSRLISYGPCQTPTLGFCVQRHLQITTFKPEKFWSVCPYIMQN 245
           AF+RFQT++FQGKY NLD R+ISYGPCQTPTLGFCVQR++ I TFKPEKFW++ PYI ++
Sbjct: 187 AFSRFQTSYFQGKYQNLDCRVISYGPCQTPTLGFCVQRYMHINTFKPEKFWALRPYIRKD 246

Query: 246 GYELNLDWERGKLFDCDVTAMFQKRVMEDGIAEVTEISEKQENKVRPSGLNTVNLLKVAS 305
           GYEL L+WER +LFD +   +FQK V+E   A+V ++SEKQE K RP+GLNTVNLLKVAS
Sbjct: 247 GYELQLEWERRRLFDLEAATVFQKLVVEGRTAKVMDVSEKQEVKGRPAGLNTVNLLKVAS 306

Query: 306 SALGFGPQMAMQLAERLYTQGFISYPRTESTAYPSSFDFKGTLHSLVKNPEWGDYVRMLL 365
           SALGFGPQ AM LAERLYTQGFISYPRTESTAYPSSFDF  TL + V NP WG YV+ LL
Sbjct: 307 SALGFGPQTAMHLAERLYTQGFISYPRTESTAYPSSFDFTDTLRAQVSNPVWGGYVQRLL 366

Query: 366 SDGYSKPRSGTDVGDHPPITPMGLATEDMLGRDAWRLYQYVCQHFIGSLSSDCKYIRTKI 425
           SDG+  P+SGTD GDHPPITPM  ATE M+G DAWRLYQYVCQHF+G++S +CKYIRTK+
Sbjct: 367 SDGFHMPKSGTDAGDHPPITPMRAATEVMVGGDAWRLYQYVCQHFLGTVSPNCKYIRTKV 426

Query: 426 QFSIAGEIFNCTGSRVMVKGFTSIMPWLAVNEKKLPQFLKGEKIEVARVELYQGNTTPPD 485
           + SI GE F+CTG RV  KGFT+IMPW AV+EKKLP FLKGE+IEV RVELY+GNT PPD
Sbjct: 427 ELSIGGETFHCTGQRVTEKGFTAIMPWSAVDEKKLPSFLKGERIEVLRVELYEGNTAPPD 486

Query: 486 YLSESELISLMEKHGIGTDASIPVHINNIGERNYVQVQAGRRLVPTNLGITLIRGYQCID 545
           YL+ESELISLMEKHGIGTDASI VHINNIGERNYVQVQ+GR++VPT LGITLIRGYQCID
Sbjct: 487 YLTESELISLMEKHGIGTDASIAVHINNIGERNYVQVQSGRKMVPTALGITLIRGYQCID 546

Query: 546 PDLCLPDIRSFIEQQITLVAKGQADHSSVVQNVLQQFKQKYSYFVKKIDHMDALFEAQFS 605
           PDLCLPDIRSFIEQQITLVAKGQADHS VVQ+V+QQF++K+SYFV++I+HMDALFEAQFS
Sbjct: 547 PDLCLPDIRSFIEQQITLVAKGQADHSHVVQHVIQQFRRKFSYFVQQIEHMDALFEAQFS 606

Query: 606 PLADSGRALSKCGKCLRYMKFISSQPQRLYCGTCEEVYYLPQKGAIKLYKELTCPLDNFE 665
           PLADSGRALSKCGKCLRYMK I++ P RL+CGTCEEVYYLPQKG +KLYKELTCPLDNFE
Sbjct: 607 PLADSGRALSKCGKCLRYMKHITAVPPRLFCGTCEEVYYLPQKGTVKLYKELTCPLDNFE 666

Query: 666 LLIFSLPGPEGKSFPLCPYCYNSPPFEGINTLYGAAKTNPSSGASSKGMGMPCFLCPHPT 725
           L+I+S+PGPEGKSFPLCPYCYNSPPFEGI+TL+GA+KT  +   +  G GMPC LCPHPT
Sbjct: 667 LVIYSVPGPEGKSFPLCPYCYNSPPFEGIDTLFGASKTPNAPAKTKTGAGMPCSLCPHPT 726

Query: 726 CRHSLITQGVCACPECSGTLVLDPVSAPNWKLNCNKCNCLVFLPQGAHRISTTNEKCADC 785
           C+HS+  QGVCACPEC GTLVLDPVS P WKLNCN C+C+V LP+GAHRI+TT+ +C +C
Sbjct: 727 CQHSVRNQGVCACPECEGTLVLDPVSFPKWKLNCNLCSCIVLLPEGAHRITTTSNRCPEC 786

Query: 786 DSTIIEVDFNKKTTPLEDGATLFVGCILCDEFLHSLLEMKHGKSFFRGRGSRGRGRGRGR 845
           DS IIE+DFNKKTTPLE+GATL  GC+LCDE L SL+E+KHG+SF R RG RGRGRGRGR
Sbjct: 787 DSAIIEIDFNKKTTPLENGATLHQGCVLCDELLLSLVEVKHGRSFVR-RGGRGRGRGRGR 846

Query: 846 GRGGRGRGRNSGKLMDPKMSFRDF 870
           GRG    GR   K +DPKMSFRDF
Sbjct: 847 GRG----GRRGSKSVDPKMSFRDF 865

BLAST of Cla97C05G088880 vs. TAIR 10
Match: AT2G32000.2 (DNA topoisomerase, type IA, core )

HSP 1 Score: 1307.0 bits (3381), Expect = 0.0e+00
Identity = 615/814 (75.55%), Postives = 703/814 (86.36%), Query Frame = 0

Query: 59  HVFSI---DFPAGFQDWKTTDPLDLFQASVIKTVSNPKAHIRQHLSREARGCDCLVLWLD 118
           H+ S+   DFP  +Q+W T DP DLF A +IK  SNPKAHI +HLS EARGC  +VLWLD
Sbjct: 30  HLLSVMFSDFPEKYQNWATIDPQDLFDAPIIKKESNPKAHICRHLSNEARGCSYMVLWLD 89

Query: 119 CDREGENICFEVIECTGFGGNEIRKKVYRARFSSVTEKDILKALDNLVQPNRNEALAVDA 178
           CDREGENICFEVIE TGF   + ++KVYRARFSSVTEKDI KA+DNLV+PNR+EALAVDA
Sbjct: 90  CDREGENICFEVIESTGFDMKDSKRKVYRARFSSVTEKDISKAMDNLVEPNRDEALAVDA 149

Query: 179 RQEIDLKVGVAFTRFQTTFFQGKYGNLDSRLISYGPCQTPTLGFCVQRHLQITTFKPEKF 238
           RQEIDLKVGVAF+RFQT++FQGKY NLD R+ISYGPCQTPTLGFCVQR++ I TFKPEKF
Sbjct: 150 RQEIDLKVGVAFSRFQTSYFQGKYQNLDCRVISYGPCQTPTLGFCVQRYMHINTFKPEKF 209

Query: 239 WSVCPYIMQNGYELNLDWERGKLFDCDVTAMFQKRVMEDGIAEVTEISEKQENKVRPSGL 298
           W++ PYI ++GYEL L+WER +LFD +   +FQK V+E   A+V ++SEKQE K RP+GL
Sbjct: 210 WALRPYIRKDGYELQLEWERRRLFDLEAATVFQKLVVEGRTAKVMDVSEKQEVKGRPAGL 269

Query: 299 NTVNLLKVASSALGFGPQMAMQLAERLYTQGFISYPRTESTAYPSSFDFKGTLHSLVKNP 358
           NTVNLLKVASSALGFGPQ AM LAERLYTQGFISYPRTESTAYPSSFDF  TL + V NP
Sbjct: 270 NTVNLLKVASSALGFGPQTAMHLAERLYTQGFISYPRTESTAYPSSFDFTDTLRAQVSNP 329

Query: 359 EWGDYVRMLLSDGYSKPRSGTDVGDHPPITPMGLATEDMLGRDAWRLYQYVCQHFIGSLS 418
            WG YV+ LLSDG+  P+SGTD GDHPPITPM  ATE M+G DAWRLYQYVCQHF+G++S
Sbjct: 330 VWGGYVQRLLSDGFHMPKSGTDAGDHPPITPMRAATEVMVGGDAWRLYQYVCQHFLGTVS 389

Query: 419 SDCKYIRTKIQFSIAGEIFNCTGSRVMVKGFTSIMPWLAVNEKKLPQFLKGEKIEVARVE 478
            +CKYIRTK++ SI GE F+CTG RV  KGFT+IMPW AV+EKKLP FLKGE+IEV RVE
Sbjct: 390 PNCKYIRTKVELSIGGETFHCTGQRVTEKGFTAIMPWSAVDEKKLPSFLKGERIEVLRVE 449

Query: 479 LYQGNTTPPDYLSESELISLMEKHGIGTDASIPVHINNIGERNYVQVQAGRRLVPTNLGI 538
           LY+GNT PPDYL+ESELISLMEKHGIGTDASI VHINNIGERNYVQVQ+GR++VPT LGI
Sbjct: 450 LYEGNTAPPDYLTESELISLMEKHGIGTDASIAVHINNIGERNYVQVQSGRKMVPTALGI 509

Query: 539 TLIRGYQCIDPDLCLPDIRSFIEQQITLVAKGQADHSSVVQNVLQQFKQKYSYFVKKIDH 598
           TLIRGYQCIDPDLCLPDIRSFIEQQITLVAKGQADHS VVQ+V+QQF++K+SYFV++I+H
Sbjct: 510 TLIRGYQCIDPDLCLPDIRSFIEQQITLVAKGQADHSHVVQHVIQQFRRKFSYFVQQIEH 569

Query: 599 MDALFEAQFSPLADSGRALSKCGKCLRYMKFISSQPQRLYCGTCEEVYYLPQKGAIKLYK 658
           MDALFEAQFSPLADSGRALSKCGKCLRYMK I++ P RL+CGTCEEVYYLPQKG +KLYK
Sbjct: 570 MDALFEAQFSPLADSGRALSKCGKCLRYMKHITAVPPRLFCGTCEEVYYLPQKGTVKLYK 629

Query: 659 ELTCPLDNFELLIFSLPGPEGKSFPLCPYCYNSPPFEGINTLYGAAKTNPSSGASSKGMG 718
           ELTCPLDNFEL+I+S+PGPEGKSFPLCPYCYNSPPFEGI+TL+GA+KT  +   +  G G
Sbjct: 630 ELTCPLDNFELVIYSVPGPEGKSFPLCPYCYNSPPFEGIDTLFGASKTPNAPAKTKTGAG 689

Query: 719 MPCFLCPHPTCRHSLITQGVCACPECSGTLVLDPVSAPNWKLNCNKCNCLVFLPQGAHRI 778
           MPC LCPHPTC+HS+  QGVCACPEC GTLVLDPVS P WKLNCN C+C+V LP+GAHRI
Sbjct: 690 MPCSLCPHPTCQHSVRNQGVCACPECEGTLVLDPVSFPKWKLNCNLCSCIVLLPEGAHRI 749

Query: 779 STTNEKCADCDSTIIEVDFNKKTTPLEDGATLFVGCILCDEFLHSLLEMKHGKSFFRGRG 838
           +TT+ +C +CDS IIE+DFNKKTTPLE+GATL  GC+LCDE L SL+E+KHG+SF R RG
Sbjct: 750 TTTSNRCPECDSAIIEIDFNKKTTPLENGATLHQGCVLCDELLLSLVEVKHGRSFVR-RG 809

Query: 839 SRGRGRGRGRGRGGRGRGRNSGKLMDPKMSFRDF 870
            RGRGRGRGRGRG    GR   K +DPKMSFRDF
Sbjct: 810 GRGRGRGRGRGRG----GRRGSKSVDPKMSFRDF 838

BLAST of Cla97C05G088880 vs. TAIR 10
Match: AT5G63920.1 (topoisomerase 3alpha )

HSP 1 Score: 403.7 bits (1036), Expect = 3.8e-112
Identity = 235/640 (36.72%), Postives = 347/640 (54.22%), Query Frame = 0

Query: 5   TVLMVAEKPSIALSIASALSHGQMSTRKGST---DVHEFDGRFLGHHAHYKVTSVIGHVF 64
           TVL VAEKPS+A S+A  LS G   TR+G +    + EFD    G      +TSVIGH+ 
Sbjct: 10  TVLNVAEKPSVAKSVAGILSRGTFRTREGRSRYNKIFEFDYAINGQPCRMLMTSVIGHLM 69

Query: 65  SIDFPAGFQDWKTTDPLDLFQASVIKTVSNPKAHIRQHLSREARGCDCLVLWLDCDREGE 124
            ++F   ++ W + DP DL+QA V+K V   K  I++ L  EAR  D LVLWLDCDREGE
Sbjct: 70  ELEFADRYRKWHSCDPADLYQAPVMKHVPEDKKDIKKTLEEEARKSDWLVLWLDCDREGE 129

Query: 125 NICFEVIE-CTGFGGNEIRKKVYRARFSSVTEKDILKALDNLVQPNRNEALAVDARQEID 184
           NI FEV++ C     N     + RA FS++ ++DI +A+ NL  PN+  A AVDARQEID
Sbjct: 130 NIAFEVVDVCRAVKHNLF---IRRAHFSALIDRDIHEAVQNLRDPNQLFAEAVDARQEID 189

Query: 185 LKVGVAFTRFQTTFFQGKY-----GNLDSRLISYGPCQTPTLGFCVQRHLQITTFKPEKF 244
           L++G +FTRFQT   + ++     G   SR+ISYGPCQ PTLGF V+R+ +I   +PE+F
Sbjct: 190 LRIGASFTRFQTMLLRDRFAIDSTGEERSRVISYGPCQFPTLGFIVERYWEIQAHEPEEF 249

Query: 245 WSV-CPYIMQNGYELNLDWERGKLFDCDVTAMFQKRVMEDGIAEVTEISEKQEN-KVRPS 304
           W++ C +  + G     +W RG LFD     +  +  +E+  A V  +   +E  K  P 
Sbjct: 250 WTINCSHQSEEGL-ATFNWMRGHLFDYASAVILYEMCVEEPTATVMNVPHPRERFKYPPY 309

Query: 305 GLNTVNLLKVASSALGFGPQMAMQLAERLYTQGFISYPRTESTAYPSSFDFKGTLHSLVK 364
            LNT+ L K AS       +  M++AE LY  GFISYPRTE+ ++ S  D +  +    +
Sbjct: 310 PLNTIELEKRASRYFRLSSEHTMKVAEELYQAGFISYPRTETDSFSSRTDLRAMVEEQTR 369

Query: 365 NPEWGDYVRMLLSD----GYSKPRSGTDVGDHPPITPMGLAT-EDMLGRDAWRLYQYVCQ 424
           +P WG Y + LL        +    G D   HPPI P   ++ E    RD   +Y+ V +
Sbjct: 370 HPAWGSYAQRLLEPEGGLWRNPANGGHDDKAHPPIHPTKFSSGESNWSRDHLNVYELVVR 429

Query: 425 HFIGSLSSDCKYIRTKIQFSIAGEIFNCTGSRVMVKGFTSIMPWLAVNEKKLPQFLKGEK 484
           H++  +S       T ++  IAGE F+ +G  ++ K +  +  + +     +P + KG++
Sbjct: 430 HYLACVSQPAVAAETTVEIDIAGERFSASGRAILAKNYLEVYRFESWGGSVIPVYEKGQQ 489

Query: 485 IEVARVELYQGNTTPPDYLSESELISLMEKHGIGTDASIPVHINNIGERNYVQVQAGRRL 544
                + L    T PP  L E++L+S M+K GIGTDA++  HI  + +R Y    A  R 
Sbjct: 490 FIPTTLTLDAAVTRPPPLLCEADLLSCMDKAGIGTDATMHDHIKKLLDRGYATKDANTRF 549

Query: 545 VPTNLGITLIRGYQCIDPDLCLPDIRSFIEQQITLVAKGQADHSSVVQNVLQQFKQKY-S 604
            PTNLG  L+ GY  +  +L  P++R+ +E  +  V+ G+   + V++  LQQ K  +  
Sbjct: 550 SPTNLGEALVMGYDDMGYELWKPNLRALMEHDMNEVSVGRKTKAEVLETCLQQMKACFLD 609

Query: 605 YFVKK---IDHMDALFE----AQFSPLADSGRALSKCGKC 621
             VKK   ++ M   FE       S    +G  + +C  C
Sbjct: 610 ARVKKSKLLEAMTIFFERSNNTDESESQTAGEVVRRCNLC 645