Cla97C05G080087 (gene) Watermelon (97103) v2.5

Overview


Name	Cla97C05G080087
Type	gene
Organism	Citrullus lanatus subsp. vulgaris cv. 97103 (Watermelon (97103) v2.5)
Description	Reverse transcriptase
Location	Cla97Chr05: 178174 .. 178749 (+)
RNA-Seq Expression	Cla97C05G080087
Synteny	Cla97C05G080087

Sequences

The following sequences are available for this feature:

Gene sequence (with intron)

Legend: exonCDSpolypeptide

Hold the cursor over a type above to highlight its positions in the sequence below.

ATGACGACTCAGTTGTTCTTCAAGCACATTGTGAAACTATGGAGTGTCTCTGTCAGTATCATTAGTGATCGTGATGCAAGATTCACTAGTACATTTTGGACGGAACTATTTCGGATATTGGGTTCGACACTGAATATTTCATCCAGTTATCATCCACAGACTGATGGACAGACGGAACGCTTCAACATATGCTCGAAAAGTATCTTCGACACTTGATTGATGCGAGAAAGAAGAATTGGGTCCAGAAGTTGGACGTGGCTCAGTTTAATCTTAACAGCCAAAAGAGTTCCTTCACAAGAAAGACGCCCTTCGAGATTGTGTGTGGTAGATAGCTCATGCCACACGTTATTGACCACCCATACGTGGGTAAGAGCCCTCAGGTGCACAATTTCACCAGAGAATAGAAAGAGTCCTCGGAAGTTGTGTGTGTGTCTAGAGAAGGCATTGAAGCGCATGAAGAAGTGGGCAGATATGAAACGCAGACCCCTCAAGTTTCAATTAGGGGTGTGCACGGTTCGGTTCGGTCTGGTTTTTAGCCGAACCATGAACCAAACCGAAGTTATCCGTTCCCATTGA

mRNA sequence

ATGACGACTCAGTTGTTCTTCAAGCACATTGTGAAACTATGGAGTGTCTCTGTCAGTATCATTAGTGATCGTGATGCAAGATTCACTAGTACATTTTGGACGGAACTATTTCGGATATTGGGTTCGACACTGAATATTTCATCCAGTTATCATCCACAGACTGATGGACAGACGGAACGCTTCAACATATGCTCGAAAACCAAAAGAGTTCCTTCACAAGAAAGACGCCCTTCGAGATTGTGTGTGGTAGATAGCTCATGCCACACGTTATTGACCACCCATACGTGGGTAAGAGCCCTCAGGTGCACAATTTCACCAGAGAATAGAAAGAGTCCTCGGAAGTTGTGTGTGTGTCTAGAGAAGGCATTGAAGCGCATGAAGAAGTGGGCAGATATGAAACGCAGACCCCTCAAGTTTCAATTAGGGGTGTGCACGGTTCGGTTCGGTCTGGTTTTTAGCCGAACCATGAACCAAACCGAAGTTATCCGTTCCCATTGA

Coding sequence (CDS)

ATGACGACTCAGTTGTTCTTCAAGCACATTGTGAAACTATGGAGTGTCTCTGTCAGTATCATTAGTGATCGTGATGCAAGATTCACTAGTACATTTTGGACGGAACTATTTCGGATATTGGGTTCGACACTGAATATTTCATCCAGTTATCATCCACAGACTGATGGACAGACGGAACGCTTCAACATATGCTCGAAAACCAAAAGAGTTCCTTCACAAGAAAGACGCCCTTCGAGATTGTGTGTGGTAGATAGCTCATGCCACACGTTATTGACCACCCATACGTGGGTAAGAGCCCTCAGGTGCACAATTTCACCAGAGAATAGAAAGAGTCCTCGGAAGTTGTGTGTGTGTCTAGAGAAGGCATTGAAGCGCATGAAGAAGTGGGCAGATATGAAACGCAGACCCCTCAAGTTTCAATTAGGGGTGTGCACGGTTCGGTTCGGTCTGGTTTTTAGCCGAACCATGAACCAAACCGAAGTTATCCGTTCCCATTGA

Protein sequence

MTTQLFFKHIVKLWSVSVSIISDRDARFTSTFWTELFRILGSTLNISSSYHPQTDGQTERFNICSKTKRVPSQERRPSRLCVVDSSCHTLLTTHTWVRALRCTISPENRKSPRKLCVCLEKALKRMKKWADMKRRPLKFQLGVCTVRFGLVFSRTMNQTEVIRSH

Homology

[Close]

BLAST of Cla97C05G080087 vs. NCBI nr
Match: KAA0053507.1 (reverse transcriptase [Cucumis melo var. makuwa])

HSP 1 Score: 131.7 bits (330), Expect = 5.7e-27
Identity = 78/147 (53.06%), Postives = 91/147 (61.90%), Query Frame = 0


Query: 2   TTQLFFKHIVKLWSVSVSIISDRDARFTSTFWTELFRILGSTLNISSSYHPQTDGQTERF 61
           T QLFFKH+VKLW V  SI+SDRD RF  +FWTELF  LG++LNISSSYHPQTDGQTERF
Sbjct: 320 TAQLFFKHVVKLWGVPTSIVSDRDGRFIGSFWTELFSFLGTSLNISSSYHPQTDGQTERF 379

Query: 62  N--ICSKTKRVPSQERRPSRLCVVDSSCHTLLTTHTWV----RALRCTISPENRKSPRKL 121
           N   C   +   S  R P  +          L  H +V    +AL  T   E R++    
Sbjct: 380 NSQFCFNAQTSSSTGRSPFEIVSGRQPVLPHLVDHPFVGKNPQALNFT--KEWRQTNDIA 439

Query: 122 CVCLEKALKRMKKWADMKRRPLKFQLG 143
              LEKA KRMKKWAD KRRPL+F+ G
Sbjct: 440 RAYLEKASKRMKKWADKKRRPLEFRAG 464

[Close]

BLAST of Cla97C05G080087 vs. NCBI nr
Match: KAA0034360.1 (reverse transcriptase [Cucumis melo var. makuwa])

HSP 1 Score: 124.8 bits (312), Expect = 7.0e-25
Identity = 77/170 (45.29%), Postives = 98/170 (57.65%), Query Frame = 0


Query: 1   MTTQLFFKHIVKLWSVSVSIISDRDARFTSTFWTELFRILGSTLNISSSYHPQTDGQTER 60
           MT QLFFKH+VKLW V  SI+SDRD RF  +FWTELF +LG++LNISSSYHPQTDGQTER
Sbjct: 241 MTAQLFFKHVVKLWGVPTSIVSDRDGRFIGSFWTELFSLLGTSLNISSSYHPQTDGQTER 300

Query: 61  FNICSK---TKRVPSQERRPSRLCVVDSSCHTLLTTHTWVRALRCTIS------------ 120
           FN   +      V ++++   +L  +   C    T+ +  R+    +S            
Sbjct: 301 FNCMLEEYLRHFVNARQKNWVQLLGIAQFCFNAQTSSSTGRSPFEIVSRRQPVLPHLVDH 360

Query: 121 PENRKSPRKL-------------CVCLEKALKRMKKWADMKRRPLKFQLG 143
           P   K+P+ L                LEKA KRMKKWAD KRRPL+F+ G
Sbjct: 361 PYAGKNPQALNFTKEWKQTNDIPRAYLEKASKRMKKWADKKRRPLEFRAG 410

[Close]

BLAST of Cla97C05G080087 vs. NCBI nr
Match: TYK15559.1 (reverse transcriptase [Cucumis melo var. makuwa])

HSP 1 Score: 124.8 bits (312), Expect = 7.0e-25
Identity = 77/170 (45.29%), Postives = 98/170 (57.65%), Query Frame = 0


Query: 1   MTTQLFFKHIVKLWSVSVSIISDRDARFTSTFWTELFRILGSTLNISSSYHPQTDGQTER 60
           MT QLFFKH+VKLW V  SI+SDRD RF  +FWTELF +LG++LNISSSYHPQTDGQTER
Sbjct: 241 MTAQLFFKHVVKLWGVPTSIVSDRDGRFIGSFWTELFSLLGTSLNISSSYHPQTDGQTER 300

Query: 61  FNICSK---TKRVPSQERRPSRLCVVDSSCHTLLTTHTWVRALRCTIS------------ 120
           FN   +      V ++++   +L  +   C    T+ +  R+    +S            
Sbjct: 301 FNCMLEEYLRHFVNARQKNWVQLLGIAQFCFNAQTSSSTGRSPFEIVSRRQPVLPHLVDH 360

Query: 121 PENRKSPRKL-------------CVCLEKALKRMKKWADMKRRPLKFQLG 143
           P   K+P+ L                LEKA KRMKKWAD KRRPL+F+ G
Sbjct: 361 PYAGKNPQALNFTKEWKQTNDIPRAYLEKASKRMKKWADKKRRPLEFRAG 410

[Close]

BLAST of Cla97C05G080087 vs. NCBI nr
Match: KAA0046544.1 (reverse transcriptase [Cucumis melo var. makuwa])

HSP 1 Score: 124.8 bits (312), Expect = 7.0e-25
Identity = 80/159 (50.31%), Postives = 90/159 (56.60%), Query Frame = 0


Query: 2    TTQLFFKHIVKLWSVSVSIISDRDARFTSTFWTELFRILGSTLNISSSYHPQTDGQTERF 61
            T QLFFKHIVKLW V  SI+SDRD RF  +FWTELF  LG++LNISSSYHPQTDGQTERF
Sbjct: 1210 TAQLFFKHIVKLWGVPTSIVSDRDGRFIGSFWTELFSFLGTSLNISSSYHPQTDGQTERF 1269

Query: 62   N----------ICSKTKRVPSQ---ERRPSRLCVVD-----SSCHTLLTTHTWVRALRCT 121
            N            S T R P +    R+P    +VD      S   L  T  W       
Sbjct: 1270 NSMLEEYLRHFTSSSTGRSPFEIVSGRQPVLPHLVDHPFAGKSPQALNFTKEW------- 1329

Query: 122  ISPENRKSPRKLCVCLEKALKRMKKWADMKRRPLKFQLG 143
                 R+        LEKA KRMKKWAD KRRPL+F+ G
Sbjct: 1330 -----RQKNDIARAYLEKASKRMKKWADKKRRPLEFRAG 1356

[Close]

BLAST of Cla97C05G080087 vs. NCBI nr
Match: TYK01597.1 (reverse transcriptase [Cucumis melo var. makuwa])

HSP 1 Score: 124.0 bits (310), Expect = 1.2e-24
Identity = 78/170 (45.88%), Postives = 97/170 (57.06%), Query Frame = 0


Query: 1    MTTQLFFKHIVKLWSVSVSIISDRDARFTSTFWTELFRILGSTLNISSSYHPQTDGQTER 60
            MT QLFFKH+VKLW V  SI+SDRD RF  +FWTELF  LG++LNISSSYHPQTDGQTER
Sbjct: 1245 MTAQLFFKHVVKLWGVPTSIVSDRDGRFIGSFWTELFSFLGTSLNISSSYHPQTDGQTER 1304

Query: 61   FNICSK---TKRVPSQERRPSRLCVVDSSCHTLLTTHTWVRALRCTIS------------ 120
            FN   +      V ++++   +L  V   C    T+ +  R+    +S            
Sbjct: 1305 FNCMLEEYLRHFVNARQKNWVQLLDVAQFCFNAQTSSSTGRSPFEIVSGRQPVLPHLVDH 1364

Query: 121  PENRKSPRKL-------------CVCLEKALKRMKKWADMKRRPLKFQLG 143
            P   K+P+ L                LEKA KRMKKWAD KRRPL+F+ G
Sbjct: 1365 PFAGKNPQALNFTKEWKQTNDIARAYLEKASKRMKKWADKKRRPLEFRAG 1414

[Close]

BLAST of Cla97C05G080087 vs. ExPASy Swiss-Prot
Match: Q99315 (Transposon Ty3-G Gag-Pol polyprotein OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=TY3B-G PE=1 SV=3)

HSP 1 Score: 47.4 bits (111), Expect = 1.9e-04
Identity = 25/56 (44.64%), Postives = 34/56 (60.71%), Query Frame = 0


Query: 5    LFFKHIVKLWSVSVSIISDRDARFTSTFWTELFRILGSTLNISSSYHPQTDGQTER 61
            L F++I        +I SDRD R T+  + EL + LG    +SS+ HPQTDGQ+ER
Sbjct: 1218 LLFRYIFSYHGFPRTITSDRDVRMTADKYQELTKRLGIKSTMSSANHPQTDGQSER 1273

[Close]

BLAST of Cla97C05G080087 vs. ExPASy Swiss-Prot
Match: Q7LHG5 (Transposon Ty3-I Gag-Pol polyprotein OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=TY3B-I PE=1 SV=2)

HSP 1 Score: 47.4 bits (111), Expect = 1.9e-04
Identity = 25/56 (44.64%), Postives = 34/56 (60.71%), Query Frame = 0


Query: 5    LFFKHIVKLWSVSVSIISDRDARFTSTFWTELFRILGSTLNISSSYHPQTDGQTER 61
            L F++I        +I SDRD R T+  + EL + LG    +SS+ HPQTDGQ+ER
Sbjct: 1244 LLFRYIFSYHGFPRTITSDRDVRMTADKYQELTKRLGIKSTMSSANHPQTDGQSER 1299

[Close]

BLAST of Cla97C05G080087 vs. ExPASy TrEMBL
Match: A0A5A7UG93 (Reverse transcriptase OS=Cucumis melo var. makuwa OX=1194695 GN=E6C27_scaffold190G00640 PE=4 SV=1)

HSP 1 Score: 131.7 bits (330), Expect = 2.8e-27
Identity = 78/147 (53.06%), Postives = 91/147 (61.90%), Query Frame = 0


Query: 2   TTQLFFKHIVKLWSVSVSIISDRDARFTSTFWTELFRILGSTLNISSSYHPQTDGQTERF 61
           T QLFFKH+VKLW V  SI+SDRD RF  +FWTELF  LG++LNISSSYHPQTDGQTERF
Sbjct: 320 TAQLFFKHVVKLWGVPTSIVSDRDGRFIGSFWTELFSFLGTSLNISSSYHPQTDGQTERF 379

Query: 62  N--ICSKTKRVPSQERRPSRLCVVDSSCHTLLTTHTWV----RALRCTISPENRKSPRKL 121
           N   C   +   S  R P  +          L  H +V    +AL  T   E R++    
Sbjct: 380 NSQFCFNAQTSSSTGRSPFEIVSGRQPVLPHLVDHPFVGKNPQALNFT--KEWRQTNDIA 439

Query: 122 CVCLEKALKRMKKWADMKRRPLKFQLG 143
              LEKA KRMKKWAD KRRPL+F+ G
Sbjct: 440 RAYLEKASKRMKKWADKKRRPLEFRAG 464

[Close]

BLAST of Cla97C05G080087 vs. ExPASy TrEMBL
Match: A0A5A7TU11 (Reverse transcriptase OS=Cucumis melo var. makuwa OX=1194695 GN=E6C27_scaffold114G00640 PE=4 SV=1)

HSP 1 Score: 124.8 bits (312), Expect = 3.4e-25
Identity = 80/159 (50.31%), Postives = 90/159 (56.60%), Query Frame = 0


Query: 2    TTQLFFKHIVKLWSVSVSIISDRDARFTSTFWTELFRILGSTLNISSSYHPQTDGQTERF 61
            T QLFFKHIVKLW V  SI+SDRD RF  +FWTELF  LG++LNISSSYHPQTDGQTERF
Sbjct: 1210 TAQLFFKHIVKLWGVPTSIVSDRDGRFIGSFWTELFSFLGTSLNISSSYHPQTDGQTERF 1269

Query: 62   N----------ICSKTKRVPSQ---ERRPSRLCVVD-----SSCHTLLTTHTWVRALRCT 121
            N            S T R P +    R+P    +VD      S   L  T  W       
Sbjct: 1270 NSMLEEYLRHFTSSSTGRSPFEIVSGRQPVLPHLVDHPFAGKSPQALNFTKEW------- 1329

Query: 122  ISPENRKSPRKLCVCLEKALKRMKKWADMKRRPLKFQLG 143
                 R+        LEKA KRMKKWAD KRRPL+F+ G
Sbjct: 1330 -----RQKNDIARAYLEKASKRMKKWADKKRRPLEFRAG 1356

[Close]

BLAST of Cla97C05G080087 vs. ExPASy TrEMBL
Match: A0A5A7STR0 (Reverse transcriptase OS=Cucumis melo var. makuwa OX=1194695 GN=E6C27_scaffold65G003640 PE=4 SV=1)

HSP 1 Score: 124.8 bits (312), Expect = 3.4e-25
Identity = 77/170 (45.29%), Postives = 98/170 (57.65%), Query Frame = 0


Query: 1   MTTQLFFKHIVKLWSVSVSIISDRDARFTSTFWTELFRILGSTLNISSSYHPQTDGQTER 60
           MT QLFFKH+VKLW V  SI+SDRD RF  +FWTELF +LG++LNISSSYHPQTDGQTER
Sbjct: 241 MTAQLFFKHVVKLWGVPTSIVSDRDGRFIGSFWTELFSLLGTSLNISSSYHPQTDGQTER 300

Query: 61  FNICSK---TKRVPSQERRPSRLCVVDSSCHTLLTTHTWVRALRCTIS------------ 120
           FN   +      V ++++   +L  +   C    T+ +  R+    +S            
Sbjct: 301 FNCMLEEYLRHFVNARQKNWVQLLGIAQFCFNAQTSSSTGRSPFEIVSRRQPVLPHLVDH 360

Query: 121 PENRKSPRKL-------------CVCLEKALKRMKKWADMKRRPLKFQLG 143
           P   K+P+ L                LEKA KRMKKWAD KRRPL+F+ G
Sbjct: 361 PYAGKNPQALNFTKEWKQTNDIPRAYLEKASKRMKKWADKKRRPLEFRAG 410

[Close]

BLAST of Cla97C05G080087 vs. ExPASy TrEMBL
Match: A0A5D3CZE9 (Reverse transcriptase OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold35G00520 PE=4 SV=1)

HSP 1 Score: 124.8 bits (312), Expect = 3.4e-25
Identity = 77/170 (45.29%), Postives = 98/170 (57.65%), Query Frame = 0


Query: 1   MTTQLFFKHIVKLWSVSVSIISDRDARFTSTFWTELFRILGSTLNISSSYHPQTDGQTER 60
           MT QLFFKH+VKLW V  SI+SDRD RF  +FWTELF +LG++LNISSSYHPQTDGQTER
Sbjct: 241 MTAQLFFKHVVKLWGVPTSIVSDRDGRFIGSFWTELFSLLGTSLNISSSYHPQTDGQTER 300

Query: 61  FNICSK---TKRVPSQERRPSRLCVVDSSCHTLLTTHTWVRALRCTIS------------ 120
           FN   +      V ++++   +L  +   C    T+ +  R+    +S            
Sbjct: 301 FNCMLEEYLRHFVNARQKNWVQLLGIAQFCFNAQTSSSTGRSPFEIVSRRQPVLPHLVDH 360

Query: 121 PENRKSPRKL-------------CVCLEKALKRMKKWADMKRRPLKFQLG 143
           P   K+P+ L                LEKA KRMKKWAD KRRPL+F+ G
Sbjct: 361 PYAGKNPQALNFTKEWKQTNDIPRAYLEKASKRMKKWADKKRRPLEFRAG 410

[Close]

BLAST of Cla97C05G080087 vs. ExPASy TrEMBL
Match: A0A5D3BQE4 (Reverse transcriptase OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold451G001890 PE=4 SV=1)

HSP 1 Score: 124.0 bits (310), Expect = 5.8e-25
Identity = 78/170 (45.88%), Postives = 97/170 (57.06%), Query Frame = 0


Query: 1    MTTQLFFKHIVKLWSVSVSIISDRDARFTSTFWTELFRILGSTLNISSSYHPQTDGQTER 60
            MT QLFFKH+VKLW V  SI+SDRD RF  +FWTELF  LG++LNISSSYHPQTDGQTER
Sbjct: 1245 MTAQLFFKHVVKLWGVPTSIVSDRDGRFIGSFWTELFSFLGTSLNISSSYHPQTDGQTER 1304

Query: 61   FNICSK---TKRVPSQERRPSRLCVVDSSCHTLLTTHTWVRALRCTIS------------ 120
            FN   +      V ++++   +L  V   C    T+ +  R+    +S            
Sbjct: 1305 FNCMLEEYLRHFVNARQKNWVQLLDVAQFCFNAQTSSSTGRSPFEIVSGRQPVLPHLVDH 1364

Query: 121  PENRKSPRKL-------------CVCLEKALKRMKKWADMKRRPLKFQLG 143
            P   K+P+ L                LEKA KRMKKWAD KRRPL+F+ G
Sbjct: 1365 PFAGKNPQALNFTKEWKQTNDIARAYLEKASKRMKKWADKKRRPLEFRAG 1414

The following BLAST results are available for this feature:

BLAST of Cla97C05G080087 vs. NCBI nr


Match Name	E-value	Identity	Description
KAA0053507.1	5.7e-27	53.06	reverse transcriptase [Cucumis melo var. makuwa]	[more]
KAA0034360.1	7.0e-25	45.29	reverse transcriptase [Cucumis melo var. makuwa]	[more]
TYK15559.1	7.0e-25	45.29	reverse transcriptase [Cucumis melo var. makuwa]	[more]
KAA0046544.1	7.0e-25	50.31	reverse transcriptase [Cucumis melo var. makuwa]	[more]
TYK01597.1	1.2e-24	45.88	reverse transcriptase [Cucumis melo var. makuwa]	[more]

BLAST of Cla97C05G080087 vs. ExPASy Swiss-Prot


Match Name	E-value	Identity	Description
Q99315	1.9e-04	44.64	Transposon Ty3-G Gag-Pol polyprotein OS=Saccharomyces cerevisiae (strain ATCC 20...	[more]
Q7LHG5	1.9e-04	44.64	Transposon Ty3-I Gag-Pol polyprotein OS=Saccharomyces cerevisiae (strain ATCC 20...	[more]

BLAST of Cla97C05G080087 vs. ExPASy TrEMBL


Match Name	E-value	Identity	Description
A0A5A7UG93	2.8e-27	53.06	Reverse transcriptase OS=Cucumis melo var. makuwa OX=1194695 GN=E6C27_scaffold19...	[more]
A0A5A7TU11	3.4e-25	50.31	Reverse transcriptase OS=Cucumis melo var. makuwa OX=1194695 GN=E6C27_scaffold11...	[more]
A0A5A7STR0	3.4e-25	45.29	Reverse transcriptase OS=Cucumis melo var. makuwa OX=1194695 GN=E6C27_scaffold65...	[more]
A0A5D3CZE9	3.4e-25	45.29	Reverse transcriptase OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold35...	[more]
A0A5D3BQE4	5.8e-25	45.88	Reverse transcriptase OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold45...	[more]

BLAST of Cla97C05G080087 vs. ExPASy TrEMBL


Match Name	E-value	Identity	Description

InterPro

Analysis Name: InterPro Annotations of Watermelon (97103) v2.5
Date Performed: 2022-01-31


IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
IPR036397	Ribonuclease H superfamily	GENE3D	3.30.420.10		coord: 1..135 e-value: 1.2E-13 score: 52.8
None	No IPR available	PANTHER	PTHR45835	YALI0A06105P	coord: 2..62
IPR001584	Integrase, catalytic core	PROSITE	PS50994	INTEGRASE	coord: 1..62 score: 9.226715
IPR012337	Ribonuclease H-like superfamily	SUPERFAMILY	53098	Ribonuclease H-like	coord: 4..111

Relationships

The following mRNA feature(s) are a part of this gene:


Feature Name	Unique Name	Type
Cla97C05G080087.1	Cla97C05G080087.1	mRNA

GO Annotation

GO Assignments

This gene is annotated with the following GO terms.

Category	Term Accession	Term Name
biological_process	GO:0015074	DNA integration
molecular_function	GO:0003676	nucleic acid binding