Cla97C04G074420 (gene) Watermelon (97103) v2.5

Overview


Name	Cla97C04G074420
Type	gene
Organism	Citrullus lanatus subsp. vulgaris cv. 97103 (Watermelon (97103) v2.5)
Description	protein MIZU-KUSSEI 1
Location	Cla97Chr04: 22118943 .. 22119704 (-)
RNA-Seq Expression	Cla97C04G074420
Synteny	Cla97C04G074420

Sequences

The following sequences are available for this feature:

Gene sequence (with intron)

Legend: exonCDSpolypeptide

Hold the cursor over a type above to highlight its positions in the sequence below.

ATGGCCAAGATCGACGCCCTTCGTCGGTTTCTCCTCCCTTGTTTCTTTCCCCCCACCGCCACAACCGCTGCTGCCGCCTCCGCCGTTCCCAAGAAACGTCTAAGCACCTCGCTTCGCGACGACGTCGAAACCACCACCTCCGCCGCCGCCAATGGAGATCCAACCCACGACCAGGATTCCGCCCAAGATTCTCCGACCACCACTCCCGACAGCGTCACGCCGAAGTTTTCCGCCACTGCCGCCTTCGTAGCCCCACCCCGGCCTTCGAAAACCATGGTCATCGGAACCATCTTCGGCCACCGGCGTGGGCACGTGTGGTTCTGCGTCCAACACGACCGCCTTCGAAACAAGCCGTTTCTCCTCCTCGAGTTTCCGATTCTGACTCACCAACTCGTTAACGAAATGCGGTTCGGACTCGTTCGAATCGCTCTCGAGTGCAACCGTGTCGAACTCGGATTCTGCCCGCTTCGTTCGATTCCCATCTGGGCAATGTCCTGCAATGGCCGAAAACTCGGGTTCGCCGCAAAGAAAAAAGCCGGCGACTCGGTCCGGTCCATGTTGAAGACGATGCAATCTACAACGGTCGGAGCCGGTGTAATGCCATCCAGGTTCGGGTCGGGTTCGGAAGAGGTTATGTACATGAGAGCTAATTATGAGCACGTGGTTGGGAGTGCTGACTCGGAATCGTTTCACCTTATCAACCCGGACGAGTGCCCGAGTCAAGAACTCAGTATCTTCTTGCTCAGATCTCGAAATGGGTAA

mRNA sequence

ATGGCCAAGATCGACGCCCTTCGTCGGTTTCTCCTCCCTTGTTTCTTTCCCCCCACCGCCACAACCGCTGCTGCCGCCTCCGCCGTTCCCAAGAAACGTCTAAGCACCTCGCTTCGCGACGACGTCGAAACCACCACCTCCGCCGCCGCCAATGGAGATCCAACCCACGACCAGGATTCCGCCCAAGATTCTCCGACCACCACTCCCGACAGCGTCACGCCGAAGTTTTCCGCCACTGCCGCCTTCGTAGCCCCACCCCGGCCTTCGAAAACCATGGTCATCGGAACCATCTTCGGCCACCGGCGTGGGCACGTGTGGTTCTGCGTCCAACACGACCGCCTTCGAAACAAGCCGTTTCTCCTCCTCGAGTTTCCGATTCTGACTCACCAACTCGTTAACGAAATGCGGTTCGGACTCGTTCGAATCGCTCTCGAGTGCAACCGTGTCGAACTCGGATTCTGCCCGCTTCGTTCGATTCCCATCTGGGCAATGTCCTGCAATGGCCGAAAACTCGGGTTCGCCGCAAAGAAAAAAGCCGGCGACTCGGTCCGGTCCATGTTGAAGACGATGCAATCTACAACGGTCGGAGCCGGTGTAATGCCATCCAGGTTCGGGTCGGGTTCGGAAGAGGTTATGTACATGAGAGCTAATTATGAGCACGTGGTTGGGAGTGCTGACTCGGAATCGTTTCACCTTATCAACCCGGACGAGTGCCCGAGTCAAGAACTCAGTATCTTCTTGCTCAGATCTCGAAATGGGTAA

Coding sequence (CDS)

ATGGCCAAGATCGACGCCCTTCGTCGGTTTCTCCTCCCTTGTTTCTTTCCCCCCACCGCCACAACCGCTGCTGCCGCCTCCGCCGTTCCCAAGAAACGTCTAAGCACCTCGCTTCGCGACGACGTCGAAACCACCACCTCCGCCGCCGCCAATGGAGATCCAACCCACGACCAGGATTCCGCCCAAGATTCTCCGACCACCACTCCCGACAGCGTCACGCCGAAGTTTTCCGCCACTGCCGCCTTCGTAGCCCCACCCCGGCCTTCGAAAACCATGGTCATCGGAACCATCTTCGGCCACCGGCGTGGGCACGTGTGGTTCTGCGTCCAACACGACCGCCTTCGAAACAAGCCGTTTCTCCTCCTCGAGTTTCCGATTCTGACTCACCAACTCGTTAACGAAATGCGGTTCGGACTCGTTCGAATCGCTCTCGAGTGCAACCGTGTCGAACTCGGATTCTGCCCGCTTCGTTCGATTCCCATCTGGGCAATGTCCTGCAATGGCCGAAAACTCGGGTTCGCCGCAAAGAAAAAAGCCGGCGACTCGGTCCGGTCCATGTTGAAGACGATGCAATCTACAACGGTCGGAGCCGGTGTAATGCCATCCAGGTTCGGGTCGGGTTCGGAAGAGGTTATGTACATGAGAGCTAATTATGAGCACGTGGTTGGGAGTGCTGACTCGGAATCGTTTCACCTTATCAACCCGGACGAGTGCCCGAGTCAAGAACTCAGTATCTTCTTGCTCAGATCTCGAAATGGGTAA

Protein sequence

MAKIDALRRFLLPCFFPPTATTAAAASAVPKKRLSTSLRDDVETTTSAAANGDPTHDQDSAQDSPTTTPDSVTPKFSATAAFVAPPRPSKTMVIGTIFGHRRGHVWFCVQHDRLRNKPFLLLEFPILTHQLVNEMRFGLVRIALECNRVELGFCPLRSIPIWAMSCNGRKLGFAAKKKAGDSVRSMLKTMQSTTVGAGVMPSRFGSGSEEVMYMRANYEHVVGSADSESFHLINPDECPSQELSIFLLRSRNG

Homology

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BLAST of Cla97C04G074420 vs. NCBI nr
Match: XP_038880832.1 (protein MIZU-KUSSEI 1 [Benincasa hispida])

HSP 1 Score: 477.6 bits (1228), Expect = 6.5e-131
Identity = 238/253 (94.07%), Postives = 242/253 (95.65%), Query Frame = 0


Query: 1   MAKIDALRRFLLPCFFPPTATTAAAASAVPKKRLSTSLRDDVETTTSAAANGDPTHDQDS 60
           MAKID+LRRFLLPCFFPPT T A AASAVPKKRLSTSLRDDVETTTS  ANGDPT+DQDS
Sbjct: 1   MAKIDSLRRFLLPCFFPPTVTNATAASAVPKKRLSTSLRDDVETTTSTTANGDPTNDQDS 60

Query: 61  AQDSPTTTPDSVTPKFSATAAFVAPPRPSKTMVIGTIFGHRRGHVWFCVQHDRLRNKPFL 120
           AQDSP TTPD+VTPKF+ATAA  APPRPSKTMVIGTIFGHRRGHVWFCVQHDRLRNKPFL
Sbjct: 61  AQDSPATTPDNVTPKFAATAAIAAPPRPSKTMVIGTIFGHRRGHVWFCVQHDRLRNKPFL 120

Query: 121 LLEFPILTHQLVNEMRFGLVRIALECNRVELGFCPLRSIPIWAMSCNGRKLGFAAKKKAG 180
           LLEFPILTHQLVNEMRFGLVRIALECNRVELG CPLRSIPIWAMSCNGRKLGFAAKKKAG
Sbjct: 121 LLEFPILTHQLVNEMRFGLVRIALECNRVELGLCPLRSIPIWAMSCNGRKLGFAAKKKAG 180

Query: 181 DSVRSMLKTMQSTTVGAGVMPSRFGSGSEEVMYMRANYEHVVGSADSESFHLINPDECPS 240
           DSVRSMLKTMQSTTVGAGVMPS FGSGSEEVMYMRANYEHVVGSADSESFHLINPDECP 
Sbjct: 181 DSVRSMLKTMQSTTVGAGVMPSGFGSGSEEVMYMRANYEHVVGSADSESFHLINPDECPG 240

Query: 241 QELSIFLLRSRNG 254
           QELSIFLLRSRNG
Sbjct: 241 QELSIFLLRSRNG 253

[Close]

BLAST of Cla97C04G074420 vs. NCBI nr
Match: XP_004142185.2 (protein MIZU-KUSSEI 1 [Cucumis sativus] >KGN54300.1 hypothetical protein Csa_017911 [Cucumis sativus])

HSP 1 Score: 456.1 bits (1172), Expect = 2.0e-124
Identity = 231/253 (91.30%), Postives = 237/253 (93.68%), Query Frame = 0


Query: 1   MAKIDALRRFLLPCFFPPTATTAAAASAVPKKRLSTSLRDDVETTTSAAANGDPTHDQDS 60
           MAKIDALRRFLLPCFFPPTA TAAA+SAVPKKRLSTSLRDD+ETTT A  N DPTH    
Sbjct: 1   MAKIDALRRFLLPCFFPPTAATAAASSAVPKKRLSTSLRDDLETTT-ATLNEDPTH---- 60

Query: 61  AQDSPTTTPDSVTPKFSATAAFVAPPRPSKTMVIGTIFGHRRGHVWFCVQHDRLRNKPFL 120
            QDSP TTPDSVTPKF+ +A+ VAPPRPSKTMVIGTIFGHRRGHVWFCVQHDRLRNKPFL
Sbjct: 61  GQDSPATTPDSVTPKFAVSASIVAPPRPSKTMVIGTIFGHRRGHVWFCVQHDRLRNKPFL 120

Query: 121 LLEFPILTHQLVNEMRFGLVRIALECNRVELGFCPLRSIPIWAMSCNGRKLGFAAKKKAG 180
           LLEFPILTHQLVNEMRFGLVRIALECNRVELGFCPLRSIPIWAMSCNGRKLGFAAKKKAG
Sbjct: 121 LLEFPILTHQLVNEMRFGLVRIALECNRVELGFCPLRSIPIWAMSCNGRKLGFAAKKKAG 180

Query: 181 DSVRSMLKTMQSTTVGAGVMPSRFGSGSEEVMYMRANYEHVVGSADSESFHLINPDECPS 240
           + VRSMLKTMQSTTVGAGVMPS FGSGSEEVMYMRANYEHVVGSADSESFHLINPDECP 
Sbjct: 181 EPVRSMLKTMQSTTVGAGVMPSGFGSGSEEVMYMRANYEHVVGSADSESFHLINPDECPG 240

Query: 241 QELSIFLLRSRNG 254
           QELSIFLLRSRNG
Sbjct: 241 QELSIFLLRSRNG 248

[Close]

BLAST of Cla97C04G074420 vs. NCBI nr
Match: XP_008448402.1 (PREDICTED: protein MIZU-KUSSEI 1 [Cucumis melo] >KAA0045148.1 protein MIZU-KUSSEI 1 [Cucumis melo var. makuwa] >TYK23589.1 protein MIZU-KUSSEI 1 [Cucumis melo var. makuwa])

HSP 1 Score: 453.8 bits (1166), Expect = 1.0e-123
Identity = 230/253 (90.91%), Postives = 237/253 (93.68%), Query Frame = 0


Query: 1   MAKIDALRRFLLPCFFPPTATTAAAASAVPKKRLSTSLRDDVETTTSAAANGDPTHDQDS 60
           MAKIDALRRFLLPCFFPPTA TAAA+S VPKKRLSTSLRDD+ETTT A  + DPTH    
Sbjct: 1   MAKIDALRRFLLPCFFPPTAATAAASSTVPKKRLSTSLRDDLETTT-ATVSEDPTH---- 60

Query: 61  AQDSPTTTPDSVTPKFSATAAFVAPPRPSKTMVIGTIFGHRRGHVWFCVQHDRLRNKPFL 120
           AQDSP TTPDSVTPKF+ +A+ VAPPRPSKTMVIGTIFGHRRGHVWFCVQHDRLRNKPFL
Sbjct: 61  AQDSPATTPDSVTPKFAISASIVAPPRPSKTMVIGTIFGHRRGHVWFCVQHDRLRNKPFL 120

Query: 121 LLEFPILTHQLVNEMRFGLVRIALECNRVELGFCPLRSIPIWAMSCNGRKLGFAAKKKAG 180
           LLEFPILTHQLVNEMRFGLVRIALECNRVELGFCPLRSIPIWAMSCNGRKLGFAAKKKAG
Sbjct: 121 LLEFPILTHQLVNEMRFGLVRIALECNRVELGFCPLRSIPIWAMSCNGRKLGFAAKKKAG 180

Query: 181 DSVRSMLKTMQSTTVGAGVMPSRFGSGSEEVMYMRANYEHVVGSADSESFHLINPDECPS 240
           +SVRSMLKTMQSTTVGAGVMPS  GSGSEEVMYMRANYEHVVGSADSESFHLINPDECP 
Sbjct: 181 ESVRSMLKTMQSTTVGAGVMPSGLGSGSEEVMYMRANYEHVVGSADSESFHLINPDECPG 240

Query: 241 QELSIFLLRSRNG 254
           QELSIFLLRSRNG
Sbjct: 241 QELSIFLLRSRNG 248

[Close]

BLAST of Cla97C04G074420 vs. NCBI nr
Match: XP_022978268.1 (protein MIZU-KUSSEI 1-like [Cucurbita maxima])

HSP 1 Score: 437.2 bits (1123), Expect = 9.7e-119
Identity = 225/256 (87.89%), Postives = 228/256 (89.06%), Query Frame = 0


Query: 1   MAKIDALRRFLLPCFFPPTATTAAAASAVPKKRLSTSLRDDVETTTSAAANGDPTHDQDS 60
           MAKIDALRRF LPCFFPPTATTA AASA PKKRLSTSLRDD+E + S  ANG PTHD   
Sbjct: 1   MAKIDALRRFFLPCFFPPTATTATAASAAPKKRLSTSLRDDLEISASTTANGGPTHD--- 60

Query: 61  AQDSPTTTPDSVTPKFSATAAFVAPPRPSKTMVIGTIFGHRRGHVWFCVQHDRLRNKPFL 120
            QDSP TTPDSVTPKF   AA VAPPRPSKTMVIGTIFGHRRGHVWFCVQ DRLRNKPFL
Sbjct: 61  -QDSPATTPDSVTPKFVTAAAIVAPPRPSKTMVIGTIFGHRRGHVWFCVQQDRLRNKPFL 120

Query: 121 LLEFPILTHQLVNEMRFGLVRIALECNRVELGFCPLRSIPIWAMSCNGRKLGFAAKKKAG 180
           LLEFPILTHQLVNEMRFGLVRIALECNRVELG CPLRSIPIWAMSCNGRKLGFAAKKKAG
Sbjct: 121 LLEFPILTHQLVNEMRFGLVRIALECNRVELGLCPLRSIPIWAMSCNGRKLGFAAKKKAG 180

Query: 181 DSVRSMLKTMQSTTVGAGVMPSRFGSGS----EEVMYMRANYEHVVGSADSESFHLINPD 240
           DS+RSMLKTMQSTTVGAGVMPS FG GS    EEVMYMRANYEHVVGSADSESFHLINPD
Sbjct: 181 DSIRSMLKTMQSTTVGAGVMPSGFGFGSGSKTEEVMYMRANYEHVVGSADSESFHLINPD 240

Query: 241 ECPSQELSIFLLRSRN 253
           E P QELSIFLLRS N
Sbjct: 241 EFPGQELSIFLLRSPN 252

[Close]

BLAST of Cla97C04G074420 vs. NCBI nr
Match: KAG7033814.1 (Protein MIZU-KUSSEI 1, partial [Cucurbita argyrosperma subsp. argyrosperma])

HSP 1 Score: 434.1 bits (1115), Expect = 8.2e-118
Identity = 225/258 (87.21%), Postives = 230/258 (89.15%), Query Frame = 0


Query: 1   MAKIDALRRFLLPCFFPPTATTAAAASAVPKKRLSTSLRDDVETTTSAAANGDPTHDQDS 60
           M KIDALRRF LPCFFPPTATTA AA+A PKKRLSTSLRDD+E +TS  ANG PTHD   
Sbjct: 1   MTKIDALRRFFLPCFFPPTATTATAATAAPKKRLSTSLRDDLEISTSTTANGGPTHD--- 60

Query: 61  AQDSPTTTPDSVTPKF----SATAAFVAPPRPSKTMVIGTIFGHRRGHVWFCVQHDRLRN 120
            QDSP TTPDSVTPKF    +A AA VAPPRPSKTMVIGTIFGHRRGHVWFCVQ DRLRN
Sbjct: 61  -QDSPATTPDSVTPKFAAAAAAAAAIVAPPRPSKTMVIGTIFGHRRGHVWFCVQQDRLRN 120

Query: 121 KPFLLLEFPILTHQLVNEMRFGLVRIALECNRVELGFCPLRSIPIWAMSCNGRKLGFAAK 180
           KPFLLLE PILTHQLVNEMRFGLVRIALECNRVELG CPLRSIPIWAMSCNGRKLGFAAK
Sbjct: 121 KPFLLLELPILTHQLVNEMRFGLVRIALECNRVELGLCPLRSIPIWAMSCNGRKLGFAAK 180

Query: 181 KKAGDSVRSMLKTMQSTTVGAGVMPSRFGSGS--EEVMYMRANYEHVVGSADSESFHLIN 240
           KKAGDS+RSMLKTMQSTTVGAGVMPS FGSGS  EEVMYMRANYEHVVGSADSESFHLIN
Sbjct: 181 KKAGDSIRSMLKTMQSTTVGAGVMPSGFGSGSKTEEVMYMRANYEHVVGSADSESFHLIN 240

Query: 241 PDECPSQELSIFLLRSRN 253
           PDE P QELSIFLLRS N
Sbjct: 241 PDEFPGQELSIFLLRSPN 254

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BLAST of Cla97C04G074420 vs. ExPASy Swiss-Prot
Match: O22227 (Protein MIZU-KUSSEI 1 OS=Arabidopsis thaliana OX=3702 GN=MIZ1 PE=1 SV=1)

HSP 1 Score: 140.6 bits (353), Expect = 2.5e-32
Identity = 81/169 (47.93%), Postives = 106/169 (62.72%), Query Frame = 0


Query: 93  VIGTIFGHRRGHVWFCVQHDRLRNKPFLLLEFPILTHQLVNEMRFGLVRIALECNRVELG 152
           V GT++GH+RGHV F VQ+++ R+ P LLL+  + T  LV EM  GLVRIALEC +    
Sbjct: 129 VTGTLYGHKRGHVTFSVQYNQ-RSDPVLLLDLAMSTATLVKEMSSGLVRIALECEKRHRS 188

Query: 153 FCPLRSIPIWAMSCNGRKLGFAAKKKAG--DSVRSMLKTMQSTTVGAGVMP--------S 212
              L   P W M CNGRK G+A  +     D+   +L T+   TVGAGV+P        S
Sbjct: 189 GTKLFQEPKWTMYCNGRKCGYAVSRGGACTDTDWRVLNTVSRVTVGAGVIPTPKTIDDVS 248

Query: 213 RFGSGSE--EVMYMRANYEHVVGSADSESFHLINPDECPSQELSIFLLR 250
             GSG+E  E++YMR  +E VVGS DSE+F+++NPD+    ELSIFLLR
Sbjct: 249 GVGSGTELGELLYMRGKFERVVGSRDSEAFYMMNPDKNGGPELSIFLLR 296

[Close]

BLAST of Cla97C04G074420 vs. ExPASy TrEMBL
Match: A0A0A0KXC2 (Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_4G297500 PE=4 SV=1)

HSP 1 Score: 456.1 bits (1172), Expect = 9.8e-125
Identity = 231/253 (91.30%), Postives = 237/253 (93.68%), Query Frame = 0


Query: 1   MAKIDALRRFLLPCFFPPTATTAAAASAVPKKRLSTSLRDDVETTTSAAANGDPTHDQDS 60
           MAKIDALRRFLLPCFFPPTA TAAA+SAVPKKRLSTSLRDD+ETTT A  N DPTH    
Sbjct: 1   MAKIDALRRFLLPCFFPPTAATAAASSAVPKKRLSTSLRDDLETTT-ATLNEDPTH---- 60

Query: 61  AQDSPTTTPDSVTPKFSATAAFVAPPRPSKTMVIGTIFGHRRGHVWFCVQHDRLRNKPFL 120
            QDSP TTPDSVTPKF+ +A+ VAPPRPSKTMVIGTIFGHRRGHVWFCVQHDRLRNKPFL
Sbjct: 61  GQDSPATTPDSVTPKFAVSASIVAPPRPSKTMVIGTIFGHRRGHVWFCVQHDRLRNKPFL 120

Query: 121 LLEFPILTHQLVNEMRFGLVRIALECNRVELGFCPLRSIPIWAMSCNGRKLGFAAKKKAG 180
           LLEFPILTHQLVNEMRFGLVRIALECNRVELGFCPLRSIPIWAMSCNGRKLGFAAKKKAG
Sbjct: 121 LLEFPILTHQLVNEMRFGLVRIALECNRVELGFCPLRSIPIWAMSCNGRKLGFAAKKKAG 180

Query: 181 DSVRSMLKTMQSTTVGAGVMPSRFGSGSEEVMYMRANYEHVVGSADSESFHLINPDECPS 240
           + VRSMLKTMQSTTVGAGVMPS FGSGSEEVMYMRANYEHVVGSADSESFHLINPDECP 
Sbjct: 181 EPVRSMLKTMQSTTVGAGVMPSGFGSGSEEVMYMRANYEHVVGSADSESFHLINPDECPG 240

Query: 241 QELSIFLLRSRNG 254
           QELSIFLLRSRNG
Sbjct: 241 QELSIFLLRSRNG 248

[Close]

BLAST of Cla97C04G074420 vs. ExPASy TrEMBL
Match: A0A5D3DK02 (Protein MIZU-KUSSEI 1 OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold500G001000 PE=4 SV=1)

HSP 1 Score: 453.8 bits (1166), Expect = 4.9e-124
Identity = 230/253 (90.91%), Postives = 237/253 (93.68%), Query Frame = 0


Query: 1   MAKIDALRRFLLPCFFPPTATTAAAASAVPKKRLSTSLRDDVETTTSAAANGDPTHDQDS 60
           MAKIDALRRFLLPCFFPPTA TAAA+S VPKKRLSTSLRDD+ETTT A  + DPTH    
Sbjct: 1   MAKIDALRRFLLPCFFPPTAATAAASSTVPKKRLSTSLRDDLETTT-ATVSEDPTH---- 60

Query: 61  AQDSPTTTPDSVTPKFSATAAFVAPPRPSKTMVIGTIFGHRRGHVWFCVQHDRLRNKPFL 120
           AQDSP TTPDSVTPKF+ +A+ VAPPRPSKTMVIGTIFGHRRGHVWFCVQHDRLRNKPFL
Sbjct: 61  AQDSPATTPDSVTPKFAISASIVAPPRPSKTMVIGTIFGHRRGHVWFCVQHDRLRNKPFL 120

Query: 121 LLEFPILTHQLVNEMRFGLVRIALECNRVELGFCPLRSIPIWAMSCNGRKLGFAAKKKAG 180
           LLEFPILTHQLVNEMRFGLVRIALECNRVELGFCPLRSIPIWAMSCNGRKLGFAAKKKAG
Sbjct: 121 LLEFPILTHQLVNEMRFGLVRIALECNRVELGFCPLRSIPIWAMSCNGRKLGFAAKKKAG 180

Query: 181 DSVRSMLKTMQSTTVGAGVMPSRFGSGSEEVMYMRANYEHVVGSADSESFHLINPDECPS 240
           +SVRSMLKTMQSTTVGAGVMPS  GSGSEEVMYMRANYEHVVGSADSESFHLINPDECP 
Sbjct: 181 ESVRSMLKTMQSTTVGAGVMPSGLGSGSEEVMYMRANYEHVVGSADSESFHLINPDECPG 240

Query: 241 QELSIFLLRSRNG 254
           QELSIFLLRSRNG
Sbjct: 241 QELSIFLLRSRNG 248

[Close]

BLAST of Cla97C04G074420 vs. ExPASy TrEMBL
Match: A0A1S3BKH4 (protein MIZU-KUSSEI 1 OS=Cucumis melo OX=3656 GN=LOC103490601 PE=4 SV=1)

HSP 1 Score: 453.8 bits (1166), Expect = 4.9e-124
Identity = 230/253 (90.91%), Postives = 237/253 (93.68%), Query Frame = 0


Query: 1   MAKIDALRRFLLPCFFPPTATTAAAASAVPKKRLSTSLRDDVETTTSAAANGDPTHDQDS 60
           MAKIDALRRFLLPCFFPPTA TAAA+S VPKKRLSTSLRDD+ETTT A  + DPTH    
Sbjct: 1   MAKIDALRRFLLPCFFPPTAATAAASSTVPKKRLSTSLRDDLETTT-ATVSEDPTH---- 60

Query: 61  AQDSPTTTPDSVTPKFSATAAFVAPPRPSKTMVIGTIFGHRRGHVWFCVQHDRLRNKPFL 120
           AQDSP TTPDSVTPKF+ +A+ VAPPRPSKTMVIGTIFGHRRGHVWFCVQHDRLRNKPFL
Sbjct: 61  AQDSPATTPDSVTPKFAISASIVAPPRPSKTMVIGTIFGHRRGHVWFCVQHDRLRNKPFL 120

Query: 121 LLEFPILTHQLVNEMRFGLVRIALECNRVELGFCPLRSIPIWAMSCNGRKLGFAAKKKAG 180
           LLEFPILTHQLVNEMRFGLVRIALECNRVELGFCPLRSIPIWAMSCNGRKLGFAAKKKAG
Sbjct: 121 LLEFPILTHQLVNEMRFGLVRIALECNRVELGFCPLRSIPIWAMSCNGRKLGFAAKKKAG 180

Query: 181 DSVRSMLKTMQSTTVGAGVMPSRFGSGSEEVMYMRANYEHVVGSADSESFHLINPDECPS 240
           +SVRSMLKTMQSTTVGAGVMPS  GSGSEEVMYMRANYEHVVGSADSESFHLINPDECP 
Sbjct: 181 ESVRSMLKTMQSTTVGAGVMPSGLGSGSEEVMYMRANYEHVVGSADSESFHLINPDECPG 240

Query: 241 QELSIFLLRSRNG 254
           QELSIFLLRSRNG
Sbjct: 241 QELSIFLLRSRNG 248

[Close]

BLAST of Cla97C04G074420 vs. ExPASy TrEMBL
Match: A0A6J1ISJ8 (protein MIZU-KUSSEI 1-like OS=Cucurbita maxima OX=3661 GN=LOC111478303 PE=4 SV=1)

HSP 1 Score: 437.2 bits (1123), Expect = 4.7e-119
Identity = 225/256 (87.89%), Postives = 228/256 (89.06%), Query Frame = 0


Query: 1   MAKIDALRRFLLPCFFPPTATTAAAASAVPKKRLSTSLRDDVETTTSAAANGDPTHDQDS 60
           MAKIDALRRF LPCFFPPTATTA AASA PKKRLSTSLRDD+E + S  ANG PTHD   
Sbjct: 1   MAKIDALRRFFLPCFFPPTATTATAASAAPKKRLSTSLRDDLEISASTTANGGPTHD--- 60

Query: 61  AQDSPTTTPDSVTPKFSATAAFVAPPRPSKTMVIGTIFGHRRGHVWFCVQHDRLRNKPFL 120
            QDSP TTPDSVTPKF   AA VAPPRPSKTMVIGTIFGHRRGHVWFCVQ DRLRNKPFL
Sbjct: 61  -QDSPATTPDSVTPKFVTAAAIVAPPRPSKTMVIGTIFGHRRGHVWFCVQQDRLRNKPFL 120

Query: 121 LLEFPILTHQLVNEMRFGLVRIALECNRVELGFCPLRSIPIWAMSCNGRKLGFAAKKKAG 180
           LLEFPILTHQLVNEMRFGLVRIALECNRVELG CPLRSIPIWAMSCNGRKLGFAAKKKAG
Sbjct: 121 LLEFPILTHQLVNEMRFGLVRIALECNRVELGLCPLRSIPIWAMSCNGRKLGFAAKKKAG 180

Query: 181 DSVRSMLKTMQSTTVGAGVMPSRFGSGS----EEVMYMRANYEHVVGSADSESFHLINPD 240
           DS+RSMLKTMQSTTVGAGVMPS FG GS    EEVMYMRANYEHVVGSADSESFHLINPD
Sbjct: 181 DSIRSMLKTMQSTTVGAGVMPSGFGFGSGSKTEEVMYMRANYEHVVGSADSESFHLINPD 240

Query: 241 ECPSQELSIFLLRSRN 253
           E P QELSIFLLRS N
Sbjct: 241 EFPGQELSIFLLRSPN 252

[Close]

BLAST of Cla97C04G074420 vs. ExPASy TrEMBL
Match: A0A6J1DF35 (protein MIZU-KUSSEI 1 OS=Momordica charantia OX=3673 GN=LOC111019486 PE=4 SV=1)

HSP 1 Score: 424.9 bits (1091), Expect = 2.4e-115
Identity = 218/254 (85.83%), Postives = 228/254 (89.76%), Query Frame = 0


Query: 1   MAKIDALRRFLLPCFFPPTA-TTAAAASAVPKKRLSTSLRDDVETTTSAAANGDPTHDQD 60
           MAKIDALRRFLLPCFFPP A  TA A SA PKKRLSTSLRDD+ETT  AA   DPT DQD
Sbjct: 1   MAKIDALRRFLLPCFFPPAAGATATATSAAPKKRLSTSLRDDIETT--AATKCDPTQDQD 60

Query: 61  SAQDSPTTTPDSVTPKFSATAAFVAPPRPSKTMVIGTIFGHRRGHVWFCVQHDRLRNKPF 120
           S Q SP TTPDS TPKF+A AA +A PRPSK+MVIGTIFG+RRGHVWFCVQHDRLR KPF
Sbjct: 61  SPQGSPATTPDSATPKFAA-AAVMAAPRPSKSMVIGTIFGNRRGHVWFCVQHDRLRTKPF 120

Query: 121 LLLEFPILTHQLVNEMRFGLVRIALECNRVELGFCPLRSIPIWAMSCNGRKLGFAAKKKA 180
           LLLEFPILTHQLVNEMRFGLVRIALEC+RVELG CPLRSIP+W MSCNGRKLGFAA+KKA
Sbjct: 121 LLLEFPILTHQLVNEMRFGLVRIALECSRVELGLCPLRSIPVWGMSCNGRKLGFAARKKA 180

Query: 181 GDSVRSMLKTMQSTTVGAGVMPSRFGSGSEEVMYMRANYEHVVGSADSESFHLINPDECP 240
           GD VR MLKTMQSTTVGAGV+PS FGSGSEEVMYMRANYEHVVGS+DSESFHLINPDECP
Sbjct: 181 GDRVRFMLKTMQSTTVGAGVIPSGFGSGSEEVMYMRANYEHVVGSSDSESFHLINPDECP 240

Query: 241 SQELSIFLLRSRNG 254
            QELSIFLLRSRNG
Sbjct: 241 GQELSIFLLRSRNG 251

[Close]

BLAST of Cla97C04G074420 vs. TAIR 10
Match: AT2G37880.1 (Protein of unknown function, DUF617 )

HSP 1 Score: 271.9 bits (694), Expect = 5.0e-73
Identity = 152/259 (58.69%), Postives = 180/259 (69.50%), Query Frame = 0


Query: 1   MAKIDALRRFLLPCFFPPT--ATTAAAASAVPKKRLSTSLRDDVETTTSAAANGDPTHDQ 60
           M KID+LRRFLLPC   PT   T +  ++   KKRLSTSLRDD++              Q
Sbjct: 1   MTKIDSLRRFLLPCISTPTNPTTVSTTSTGASKKRLSTSLRDDIDV-------------Q 60

Query: 61  DSAQDSPTTTPDSVTPKFSATAAFVAPPRPSKTMVIGTIFGHRRGHVWFCVQHDRLRNKP 120
           DSA  S +++  +    +S +A  V P RPSKTMVIGTIFG R+GHVWFCVQHDRL  KP
Sbjct: 61  DSASSSASSSEATSAVDYSISAVTV-PQRPSKTMVIGTIFGRRKGHVWFCVQHDRLSVKP 120

Query: 121 FLLLEFPILTHQLVNEMRFGLVRIALEC-NRVELGFCPLRSIPIWAMSCNGRKLGFAAKK 180
            LLLE  I T QLV+EM  GLVR+ALEC  R EL  C LRS+P+W M CNGRKLGFA ++
Sbjct: 121 ILLLELSIATSQLVHEMGSGLVRVALECPTRPELKSCLLRSVPVWTMFCNGRKLGFAVRR 180

Query: 181 KAGDSVRSMLKTMQSTTVGAGVMPSRFGSG------SEEVMYMRANYEHVVGSADSESFH 240
            A +  R MLK ++S TVGAGV+PS  G G      ++EVMYMRANYEHVVGS+DSESFH
Sbjct: 181 SANEETRMMLKRLESMTVGAGVLPSGSGLGGSDESDTDEVMYMRANYEHVVGSSDSESFH 240

Query: 241 LINPDECPSQELSIFLLRS 251
           LINPD   +QELSIFLLR+
Sbjct: 241 LINPDANSAQELSIFLLRT 245

[Close]

BLAST of Cla97C04G074420 vs. TAIR 10
Match: AT1G21050.1 (Protein of unknown function, DUF617 )

HSP 1 Score: 149.8 bits (377), Expect = 2.9e-36
Identity = 81/166 (48.80%), Postives = 108/166 (65.06%), Query Frame = 0


Query: 89  SKTMVIGTIFGHRRGHVWFCVQHDRLRNKPFLLLEFPILTHQLVNEM-RFGLVRIALECN 148
           S + V GT FGHRRG V FC+Q   + + P LLLE  + T  L  EM   G++RIALEC+
Sbjct: 83  SSSTVSGTFFGHRRGRVSFCLQDAAVGSSPLLLLELAVPTAALAKEMDEEGVLRIALECD 142

Query: 149 RVELGFCPLRSI---PIWAMSCNGRKLGFAAKKKAGDSVRSMLKTMQSTTVGAGVMPSRF 208
           R         SI   P+W+M CNGRK+GFA ++K  ++    L+ MQS +VGAGV+PS  
Sbjct: 143 RRRSSNSRSSSIFDVPVWSMFCNGRKMGFAVRRKVTENDAVFLRMMQSVSVGAGVVPS-- 202

Query: 209 GSGSEEVMYMRANYEHVVGSADSESFHLINPDECPSQELSIFLLRS 251
               ++++Y+RA +E V GS+DSESFH++NP     QELSIFLLRS
Sbjct: 203 -EEEDQMLYLRARFERVTGSSDSESFHMMNPGGSYGQELSIFLLRS 245

[Close]

BLAST of Cla97C04G074420 vs. TAIR 10
Match: AT2G41660.1 (Protein of unknown function, DUF617 )

HSP 1 Score: 140.6 bits (353), Expect = 1.8e-33
Identity = 81/169 (47.93%), Postives = 106/169 (62.72%), Query Frame = 0


Query: 93  VIGTIFGHRRGHVWFCVQHDRLRNKPFLLLEFPILTHQLVNEMRFGLVRIALECNRVELG 152
           V GT++GH+RGHV F VQ+++ R+ P LLL+  + T  LV EM  GLVRIALEC +    
Sbjct: 129 VTGTLYGHKRGHVTFSVQYNQ-RSDPVLLLDLAMSTATLVKEMSSGLVRIALECEKRHRS 188

Query: 153 FCPLRSIPIWAMSCNGRKLGFAAKKKAG--DSVRSMLKTMQSTTVGAGVMP--------S 212
              L   P W M CNGRK G+A  +     D+   +L T+   TVGAGV+P        S
Sbjct: 189 GTKLFQEPKWTMYCNGRKCGYAVSRGGACTDTDWRVLNTVSRVTVGAGVIPTPKTIDDVS 248

Query: 213 RFGSGSE--EVMYMRANYEHVVGSADSESFHLINPDECPSQELSIFLLR 250
             GSG+E  E++YMR  +E VVGS DSE+F+++NPD+    ELSIFLLR
Sbjct: 249 GVGSGTELGELLYMRGKFERVVGSRDSEAFYMMNPDKNGGPELSIFLLR 296

[Close]

BLAST of Cla97C04G074420 vs. TAIR 10
Match: AT1G76610.1 (Protein of unknown function, DUF617 )

HSP 1 Score: 137.5 bits (345), Expect = 1.5e-32
Identity = 83/192 (43.23%), Postives = 115/192 (59.90%), Query Frame = 0


Query: 62  QDSPTTTPDSVTPKFSATAAFVAPPRPSKTMVIGTIFGHRRGHVWFCVQHD-RLRNKPFL 121
           Q  PT   D+V+   S +++       +  +V GT +GHRRGHV FC+Q D R  + P L
Sbjct: 50  QPPPTDQNDTVSVSSSTSSS-----DQTGVVVTGTFYGHRRGHVSFCLQDDTRPSSPPLL 109

Query: 122 LLEFPILTHQLVNEMRFGLVRIAL--ECNRVELGFCPLRSIPIWAMSCNGRKLGFAAKKK 181
           LLE  + T  L  EM  G +RIAL  + NR    F    ++P+W+M CNGRK GFA +++
Sbjct: 110 LLELAVPTAALAREMEEGFLRIALRSKSNRRSSIF----NVPVWSMYCNGRKFGFAVRRE 169

Query: 182 AGDSVRSMLKTMQSTTVGAGVMPSRFGSGSEEVMYMRANYEHVVGSADSESFHLINPDEC 241
             ++    L+ MQS +VGAGV+P      + E +Y+RA +E V GS+DSESFH++N    
Sbjct: 170 TTENDVGFLRLMQSVSVGAGVIP------NGETLYLRAKFERVTGSSDSESFHMVNQGGG 226

Query: 242 PSQELSIFLLRS 251
             QELSIFL RS
Sbjct: 230 YGQELSIFLSRS 226

[Close]

BLAST of Cla97C04G074420 vs. TAIR 10
Match: AT3G25640.1 (Protein of unknown function, DUF617 )

HSP 1 Score: 127.9 bits (320), Expect = 1.2e-29
Identity = 75/170 (44.12%), Postives = 102/170 (60.00%), Query Frame = 0


Query: 93  VIGTIFGHRRGHVWFCVQHDRLRNKPFLLLEFPILTHQLVNEMRFGLVRIALECNRVEL- 152
           V+GT+FG+RRGHV+F VQ D  R  P +L++ P  T  LV EM  GLVRIALE    +  
Sbjct: 98  VVGTLFGNRRGHVYFAVQDDPTR-LPAVLIQLPTPTSVLVREMASGLVRIALETAAYKTD 157

Query: 153 GFCPLRSIPIWAMSCNGRKLGFAAKKKAGDSVRSMLKTMQSTTVGAGVMPS--------- 212
               L     W   CNG+K G+AA+K+ G++   +LK +   T+GAGV+P+         
Sbjct: 158 SKKKLLEESTWRTYCNGKKCGYAARKECGEAEWKVLKAVGPITMGAGVLPATTTTVDEEG 217

Query: 213 --RFGSGSEEVMYMRANYEHVVGSADSESFHLINPD-ECPSQELSIFLLR 250
               GS   E+MYMRA +E VVGS DSE+F+++NPD      ELS++ LR
Sbjct: 218 NGAVGSEKGELMYMRARFERVVGSRDSEAFYMMNPDVSSGGPELSVYFLR 266

The following BLAST results are available for this feature:

BLAST of Cla97C04G074420 vs. NCBI nr


Match Name	E-value	Identity	Description
XP_038880832.1	6.5e-131	94.07	protein MIZU-KUSSEI 1 [Benincasa hispida]	[more]
XP_004142185.2	2.0e-124	91.30	protein MIZU-KUSSEI 1 [Cucumis sativus] >KGN54300.1 hypothetical protein Csa_017...	[more]
XP_008448402.1	1.0e-123	90.91	PREDICTED: protein MIZU-KUSSEI 1 [Cucumis melo] >KAA0045148.1 protein MIZU-KUSSE...	[more]
XP_022978268.1	9.7e-119	87.89	protein MIZU-KUSSEI 1-like [Cucurbita maxima]	[more]
KAG7033814.1	8.2e-118	87.21	Protein MIZU-KUSSEI 1, partial [Cucurbita argyrosperma subsp. argyrosperma]	[more]

BLAST of Cla97C04G074420 vs. ExPASy Swiss-Prot


Match Name	E-value	Identity	Description
O22227	2.5e-32	47.93	Protein MIZU-KUSSEI 1 OS=Arabidopsis thaliana OX=3702 GN=MIZ1 PE=1 SV=1	[more]

BLAST of Cla97C04G074420 vs. ExPASy TrEMBL


Match Name	E-value	Identity	Description
A0A0A0KXC2	9.8e-125	91.30	Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_4G297500 PE=4 SV=1	[more]
A0A5D3DK02	4.9e-124	90.91	Protein MIZU-KUSSEI 1 OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold50...	[more]
A0A1S3BKH4	4.9e-124	90.91	protein MIZU-KUSSEI 1 OS=Cucumis melo OX=3656 GN=LOC103490601 PE=4 SV=1	[more]
A0A6J1ISJ8	4.7e-119	87.89	protein MIZU-KUSSEI 1-like OS=Cucurbita maxima OX=3661 GN=LOC111478303 PE=4 SV=1	[more]
A0A6J1DF35	2.4e-115	85.83	protein MIZU-KUSSEI 1 OS=Momordica charantia OX=3673 GN=LOC111019486 PE=4 SV=1	[more]

BLAST of Cla97C04G074420 vs. TAIR 10


Match Name	E-value	Identity	Description
AT2G37880.1	5.0e-73	58.69	Protein of unknown function, DUF617	[more]
AT1G21050.1	2.9e-36	48.80	Protein of unknown function, DUF617	[more]
AT2G41660.1	1.8e-33	47.93	Protein of unknown function, DUF617	[more]
AT1G76610.1	1.5e-32	43.23	Protein of unknown function, DUF617	[more]
AT3G25640.1	1.2e-29	44.12	Protein of unknown function, DUF617	[more]

InterPro

Analysis Name: InterPro Annotations of Watermelon (97103) v2.5
Date Performed: 2022-01-31


IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
IPR006460	Protein MIZU-KUSSEI 1-like, plant	PFAM	PF04759	DUF617	coord: 93..249 e-value: 1.2E-58 score: 197.8
IPR006460	Protein MIZU-KUSSEI 1-like, plant	TIGRFAM	TIGR01570	TIGR01570	coord: 93..250 e-value: 4.7E-58 score: 193.6
None	No IPR available	MOBIDB_LITE	mobidb-lite	disorder_prediction	coord: 40..73
None	No IPR available	MOBIDB_LITE	mobidb-lite	disorder_prediction	coord: 34..73
None	No IPR available	PANTHER	PTHR31276:SF6	PROTEIN MIZU-KUSSEI 1	coord: 1..251
None	No IPR available	PANTHER	PTHR31276	FAMILY NOT NAMED	coord: 1..251

Relationships

The following mRNA feature(s) are a part of this gene:


Feature Name	Unique Name	Type
Cla97C04G074420.1	Cla97C04G074420.1	mRNA

GO Annotation

GO Assignments

This gene is annotated with the following GO terms.

Category	Term Accession	Term Name
biological_process	GO:0010274	hydrotropism