Cla97C03G065030 (gene) Watermelon (97103) v2.5

Overview


Name	Cla97C03G065030
Type	gene
Organism	Citrullus lanatus subsp. vulgaris cv. 97103 (Watermelon (97103) v2.5)
Description	Plant invertase/pectin methylesterase inhibitor superfamily protein
Location	Cla97Chr03: 28535907 .. 28536506 (+)
RNA-Seq Expression	Cla97C03G065030
Synteny	Cla97C03G065030

Sequences

The following sequences are available for this feature:

Gene sequence (with intron)

Legend: exonCDSpolypeptide

Hold the cursor over a type above to highlight its positions in the sequence below.

ATGACTTTCCACTCTCTCCGCCTCCTCCTCCTCCTCTCACTCTGTCTCTCTGCCGCCGCCCACGACGGCGCTGCCGTCTCCCAAGATCTGATCCGCTCCTCCTGCCTCCAAGCAAGCTACCCAAATCTCTGCGTCAGAACCCTCTCCTCCTACGGCGGCGCCGTCAACACATCACAGGATCTTGCCCAAGCCGCCATCTCCGTCAGCCTCTCCCTCGCCCGCGACCTCTCCCGGTATCTCTCCGACGCCCGCCGGCAGGCGTCGAGGCGGCAGCGCGCGGCGGTGAATGACTGCGTGGATCAGATTGGGGACTCTGTTGAGGAGCTGAGCAACACTTTGAGCGTCCTCCGGCACCTCCCCTGCGGCAGCGACCGGCGGGCTTTCCGGCTGCAGATGGGGAATGCGAAGACGTGGGTTAGCGCTGCCTTGACGAATGAATACACGTGTCTCGATGGGTTTAAAGAAGTGGAAGGGGAAGTGAAATTGGATGTGAAGAAGAGGATTTCGAAGGTGGCTAAAGTTACTAGTAATGCTCTGTTTTTTATTAATCGCCTCGACGGTGGAAACCCCGCCGGAAGGAAGAAGTTGGCCGCTCCTTGA

mRNA sequence

ATGACTTTCCACTCTCTCCGCCTCCTCCTCCTCCTCTCACTCTGTCTCTCTGCCGCCGCCCACGACGGCGCTGCCGTCTCCCAAGATCTGATCCGCTCCTCCTGCCTCCAAGCAAGCTACCCAAATCTCTGCGTCAGAACCCTCTCCTCCTACGGCGGCGCCGTCAACACATCACAGGATCTTGCCCAAGCCGCCATCTCCGTCAGCCTCTCCCTCGCCCGCGACCTCTCCCGGTATCTCTCCGACGCCCGCCGGCAGGCGTCGAGGCGGCAGCGCGCGGCGGTGAATGACTGCGTGGATCAGATTGGGGACTCTGTTGAGGAGCTGAGCAACACTTTGAGCGTCCTCCGGCACCTCCCCTGCGGCAGCGACCGGCGGGCTTTCCGGCTGCAGATGGGGAATGCGAAGACGTGGGTTAGCGCTGCCTTGACGAATGAATACACGTGTCTCGATGGGTTTAAAGAAGTGGAAGGGGAAGTGAAATTGGATGTGAAGAAGAGGATTTCGAAGGTGGCTAAAGTTACTAGTAATGCTCTGTTTTTTATTAATCGCCTCGACGGTGGAAACCCCGCCGGAAGGAAGAAGTTGGCCGCTCCTTGA

Coding sequence (CDS)

ATGACTTTCCACTCTCTCCGCCTCCTCCTCCTCCTCTCACTCTGTCTCTCTGCCGCCGCCCACGACGGCGCTGCCGTCTCCCAAGATCTGATCCGCTCCTCCTGCCTCCAAGCAAGCTACCCAAATCTCTGCGTCAGAACCCTCTCCTCCTACGGCGGCGCCGTCAACACATCACAGGATCTTGCCCAAGCCGCCATCTCCGTCAGCCTCTCCCTCGCCCGCGACCTCTCCCGGTATCTCTCCGACGCCCGCCGGCAGGCGTCGAGGCGGCAGCGCGCGGCGGTGAATGACTGCGTGGATCAGATTGGGGACTCTGTTGAGGAGCTGAGCAACACTTTGAGCGTCCTCCGGCACCTCCCCTGCGGCAGCGACCGGCGGGCTTTCCGGCTGCAGATGGGGAATGCGAAGACGTGGGTTAGCGCTGCCTTGACGAATGAATACACGTGTCTCGATGGGTTTAAAGAAGTGGAAGGGGAAGTGAAATTGGATGTGAAGAAGAGGATTTCGAAGGTGGCTAAAGTTACTAGTAATGCTCTGTTTTTTATTAATCGCCTCGACGGTGGAAACCCCGCCGGAAGGAAGAAGTTGGCCGCTCCTTGA

Protein sequence

MTFHSLRLLLLLSLCLSAAAHDGAAVSQDLIRSSCLQASYPNLCVRTLSSYGGAVNTSQDLAQAAISVSLSLARDLSRYLSDARRQASRRQRAAVNDCVDQIGDSVEELSNTLSVLRHLPCGSDRRAFRLQMGNAKTWVSAALTNEYTCLDGFKEVEGEVKLDVKKRISKVAKVTSNALFFINRLDGGNPAGRKKLAAP

Homology

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BLAST of Cla97C03G065030 vs. NCBI nr
Match: XP_038879670.1 (pectinesterase inhibitor 3 [Benincasa hispida])

HSP 1 Score: 326.2 bits (835), Expect = 1.9e-85
Identity = 178/199 (89.45%), Postives = 181/199 (90.95%), Query Frame = 0


Query: 1   MTFHSLRLLLLLSLCLSAAAHDGAAVSQDLIRSSCLQASYPNLCVRTLSSYGGAVNTSQD 60
           MTF  L LLLLLSL LS AAHDGAAVSQDLI SSCLQA YPNLC+RTLSSY GAV T Q+
Sbjct: 33  MTF--LSLLLLLSLSLS-AAHDGAAVSQDLIHSSCLQARYPNLCIRTLSSYAGAVTTPQE 92

Query: 61  LAQAAISVSLSLARDLSRYLSDARRQASRRQRAAVNDCVDQIGDSVEELSNTLSVLRHLP 120
           LAQAAISVSLSLARDLSRYLSDA RQAS RQRAAVNDCVDQIGDSVEELSNTL VLRHL 
Sbjct: 93  LAQAAISVSLSLARDLSRYLSDALRQASMRQRAAVNDCVDQIGDSVEELSNTLGVLRHLT 152

Query: 121 CGSDRRAFRLQMGNAKTWVSAALTNEYTCLDGFKEVEGEVKLDVKKRISKVAKVTSNALF 180
           CG DRR FRLQMGNAKTWVSAALTNE TCLDGFKEV+GEVKLDVKKRISKVAKVTSNALF
Sbjct: 153 CGGDRRDFRLQMGNAKTWVSAALTNEDTCLDGFKEVDGEVKLDVKKRISKVAKVTSNALF 212

Query: 181 FINRLDGGNPAGRKKLAAP 200
            INRLDGGNPAGRKKLAAP
Sbjct: 213 MINRLDGGNPAGRKKLAAP 228

[Close]

BLAST of Cla97C03G065030 vs. NCBI nr
Match: XP_023516503.1 (pectinesterase inhibitor 3 [Cucurbita pepo subsp. pepo])

HSP 1 Score: 298.1 bits (762), Expect = 5.6e-77
Identity = 163/202 (80.69%), Postives = 176/202 (87.13%), Query Frame = 0


Query: 1   MTFHSLRLLLLLSLCLSAAAHDGAAVSQDLIRSSCLQASYPNLCVRTLSSYGGAVNTSQD 60
           MT  SL L LLLSL LSAAAHDG   SQDLI SSCLQASYP+LC+RTLSSY GAVNT QD
Sbjct: 1   MTSLSLLLFLLLSLSLSAAAHDGPPPSQDLIHSSCLQASYPSLCIRTLSSYSGAVNTPQD 60

Query: 61  LAQAAISVSLSLARDLSRYLSDAR---RQASRRQRAAVNDCVDQIGDSVEELSNTLSVLR 120
           LAQA +SVSLSLARDLSRYLS++    RQAS+RQRAAV+DCV+QIGDSVEELSNTL +LR
Sbjct: 61  LAQATLSVSLSLARDLSRYLSNSSANLRQASKRQRAAVDDCVEQIGDSVEELSNTLGLLR 120

Query: 121 HLPCGSDRRAFRLQMGNAKTWVSAALTNEYTCLDGFKEVEGEVKLDVKKRISKVAKVTSN 180
           HLP G DRR+FRLQM NA+TWVSAALTNE TCLDGFKEV+GEVK DVKKRISKVAK TSN
Sbjct: 121 HLPYG-DRRSFRLQMSNAQTWVSAALTNEDTCLDGFKEVDGEVKFDVKKRISKVAKATSN 180

Query: 181 ALFFINRLDGGNPAGRKKLAAP 200
           AL+ INRLD G PAGRKKLAAP
Sbjct: 181 ALYMINRLDVGTPAGRKKLAAP 201

[Close]

BLAST of Cla97C03G065030 vs. NCBI nr
Match: XP_022961187.1 (pectinesterase inhibitor 3 [Cucurbita moschata] >KAG6590055.1 Pectinesterase inhibitor 3, partial [Cucurbita argyrosperma subsp. sororia] >KAG7023750.1 Pectinesterase inhibitor 3, partial [Cucurbita argyrosperma subsp. argyrosperma])

HSP 1 Score: 297.0 bits (759), Expect = 1.2e-76
Identity = 162/202 (80.20%), Postives = 176/202 (87.13%), Query Frame = 0


Query: 1   MTFHSLRLLLLLSLCLSAAAHDGAAVSQDLIRSSCLQASYPNLCVRTLSSYGGAVNTSQD 60
           MT  SL L+LLLSL LSAAAHDG   SQDLI SSCLQASYP+LC+RTLSSY GAVNT QD
Sbjct: 1   MTSLSLLLVLLLSLSLSAAAHDGPPPSQDLIHSSCLQASYPSLCIRTLSSYSGAVNTPQD 60

Query: 61  LAQAAISVSLSLARDLSRYLSDAR---RQASRRQRAAVNDCVDQIGDSVEELSNTLSVLR 120
           LAQA +SVSLSLARDLSRYLS++    RQAS+RQRAAV+DCV+QIGDSVEELSNTL +LR
Sbjct: 61  LAQATLSVSLSLARDLSRYLSNSSANLRQASKRQRAAVDDCVEQIGDSVEELSNTLGLLR 120

Query: 121 HLPCGSDRRAFRLQMGNAKTWVSAALTNEYTCLDGFKEVEGEVKLDVKKRISKVAKVTSN 180
           HLP G DRR+FRLQM NA+TWVSAALTNE TCLDGFKEV+GEVK DVKKRISKVAK TSN
Sbjct: 121 HLPYG-DRRSFRLQMSNAQTWVSAALTNEDTCLDGFKEVDGEVKFDVKKRISKVAKATSN 180

Query: 181 ALFFINRLDGGNPAGRKKLAAP 200
           AL+ INRLD G PAGRKKL AP
Sbjct: 181 ALYMINRLDVGTPAGRKKLTAP 201

[Close]

BLAST of Cla97C03G065030 vs. NCBI nr
Match: XP_004144132.2 (pectinesterase inhibitor 3 [Cucumis sativus] >KGN66330.1 hypothetical protein Csa_007271 [Cucumis sativus])

HSP 1 Score: 295.0 bits (754), Expect = 4.7e-76
Identity = 159/196 (81.12%), Postives = 170/196 (86.73%), Query Frame = 0


Query: 1   MTFHSLRLLLLLSLCLSAAAHDGAAVSQDLIRSSCLQASYPNLCVRTLSSYGGAVNTSQD 60
           MT  SL LLLL SL LSAA     A+SQDLI SSCLQASYP LC+RTLSSY GAV T +D
Sbjct: 28  MTVLSL-LLLLFSLSLSAAHGGRGAISQDLIHSSCLQASYPTLCIRTLSSYAGAVKTPRD 87

Query: 61  LAQAAISVSLSLARDLSRYLSDARRQASRRQRAAVNDCVDQIGDSVEELSNTLSVLRHLP 120
           LAQA ISVSLSLA++LS YLSD+ RQASR+QRAAV+DCVDQIGDSVEELSNTL VLRHLP
Sbjct: 88  LAQATISVSLSLAQNLSEYLSDSLRQASRQQRAAVDDCVDQIGDSVEELSNTLGVLRHLP 147

Query: 121 CGSDRRAFRLQMGNAKTWVSAALTNEYTCLDGFKEVEGEVKLDVKKRISKVAKVTSNALF 180
           CG DRR FRL+MGNAKTWVSAALTNE TCLDGFKEV+GEVKLDVK+RI KVAKVTSNALF
Sbjct: 148 CGDDRRKFRLEMGNAKTWVSAALTNEETCLDGFKEVDGEVKLDVKRRILKVAKVTSNALF 207

Query: 181 FINRLDGGNPAGRKKL 197
            INRLDGGNP GRK L
Sbjct: 208 MINRLDGGNPTGRKML 222

[Close]

BLAST of Cla97C03G065030 vs. NCBI nr
Match: XP_022987422.1 (pectinesterase inhibitor 3 [Cucurbita maxima])

HSP 1 Score: 294.7 bits (753), Expect = 6.1e-76
Identity = 161/202 (79.70%), Postives = 175/202 (86.63%), Query Frame = 0


Query: 1   MTFHSLRLLLLLSLCLSAAAHDGAAVSQDLIRSSCLQASYPNLCVRTLSSYGGAVNTSQD 60
           MT  SL L LLLSL LSAAAHDG   SQDLI SSCLQASYP+LC+RTLSSY GAVNT QD
Sbjct: 1   MTSLSLLLFLLLSLSLSAAAHDGPPPSQDLIHSSCLQASYPSLCIRTLSSYSGAVNTPQD 60

Query: 61  LAQAAISVSLSLARDLSRYLSDAR---RQASRRQRAAVNDCVDQIGDSVEELSNTLSVLR 120
           LAQA +SVSLSLARDLSRYLS++    RQAS+RQR AV+DCV+QIGDSVEELSNTL +LR
Sbjct: 61  LAQATLSVSLSLARDLSRYLSNSSVNLRQASKRQRVAVDDCVEQIGDSVEELSNTLGLLR 120

Query: 121 HLPCGSDRRAFRLQMGNAKTWVSAALTNEYTCLDGFKEVEGEVKLDVKKRISKVAKVTSN 180
           HLP G DRR+FRLQM NA+TWVSAALTNE TCLDGFKEV+GEVK DVKKRISKVAK TSN
Sbjct: 121 HLPYG-DRRSFRLQMSNAQTWVSAALTNEDTCLDGFKEVDGEVKFDVKKRISKVAKATSN 180

Query: 181 ALFFINRLDGGNPAGRKKLAAP 200
           AL+ INRL+ G PAGRKKLAAP
Sbjct: 181 ALYMINRLNVGTPAGRKKLAAP 201

[Close]

BLAST of Cla97C03G065030 vs. ExPASy Swiss-Prot
Match: Q84WE4 (Pectinesterase inhibitor 3 OS=Arabidopsis thaliana OX=3702 GN=PMEI3 PE=2 SV=1)

HSP 1 Score: 165.2 bits (417), Expect = 7.4e-40
Identity = 90/171 (52.63%), Postives = 126/171 (73.68%), Query Frame = 0


Query: 21  HDGAAVSQDLIRSSCLQASYPNLCVRTLSSYGG-AVNTSQDLAQAAISVSLSLARDLSRY 80
           H+GA   +D++ SSC  ASYP+LCVRTLSSY G  +   +DLAQAAI +SLS A+  ++ 
Sbjct: 27  HNGA---EDIVHSSCEHASYPSLCVRTLSSYSGPTITNRRDLAQAAIKISLSHAQSAAKK 86

Query: 81  LSDARRQASRR--QRAAVNDCVDQIGDSVEELSNTLSVLRHL-PCGSDRRAFRLQMGNAK 140
           L+  R    ++  ++AA+ DCV+ IGDSV+ELS TL VL+HL   G   + FR QM NA+
Sbjct: 87  LAVVRDSVGKKKQEKAALVDCVEMIGDSVDELSRTLGVLKHLRVSGGSAKEFRWQMSNAQ 146

Query: 141 TWVSAALTNEYTCLDGFKEV-EGEVKLDVKKRISKVAKVTSNALFFINRLD 187
           TW SAALT++ TCLDGF+ + +GE+K +VK+ ++KVA+VTSNAL+ +N+LD
Sbjct: 147 TWASAALTDDDTCLDGFQGMDDGEIKTEVKQWMTKVARVTSNALYMVNQLD 194

[Close]

BLAST of Cla97C03G065030 vs. ExPASy Swiss-Prot
Match: Q9SI72 (Pectinesterase inhibitor 9 OS=Arabidopsis thaliana OX=3702 GN=PMEI9 PE=2 SV=1)

HSP 1 Score: 118.2 bits (295), Expect = 1.0e-25
Identity = 70/186 (37.63%), Postives = 113/186 (60.75%), Query Frame = 0


Query: 6   LRLLLLLSLCLSAAAHDGAAVSQDLIRSSCLQASYPNLCVRTLSSYGGAV--NTSQDLAQ 65
           L +LL +++  S++A    + S   I SSC    YP+LCV TLS+Y   +  N  QDLAQ
Sbjct: 9   LVILLSITILQSSSATPNRSESDQFIVSSCQTTQYPSLCVHTLSAYATKIRHNNDQDLAQ 68

Query: 66  AAISVSLSLARDLSRYLSDARRQA---SRRQRAAVNDCVDQIGDSVEELSNTLSVLRHLP 125
            A+ +SL+ A+ ++ +++   ++     RR+  A+ DC++ +G+SV+ L+ ++  L    
Sbjct: 69  TALIISLARAKSVTIFVAKLTKETPKFKRREYLAIKDCIEVLGNSVDRLAQSVKELARAG 128

Query: 126 CGSDRRAFRLQMGNAKTWVSAALTNEYTCLDGFKE--VEGEVKLDVKKRISKVAKVTSNA 185
                  F  +M N +TWVSAALT+E TCLDGF E  + G+VK  ++ ++  VA+VTSNA
Sbjct: 129 HAVASEDFMWKMSNVQTWVSAALTDETTCLDGFSERAMGGKVKRLIRYKVVHVAQVTSNA 188

[Close]

BLAST of Cla97C03G065030 vs. ExPASy Swiss-Prot
Match: Q9SB37 (Pectinesterase inhibitor 7 OS=Arabidopsis thaliana OX=3702 GN=PMEI7 PE=2 SV=1)

HSP 1 Score: 115.9 bits (289), Expect = 5.1e-25
Identity = 71/187 (37.97%), Postives = 111/187 (59.36%), Query Frame = 0


Query: 9   LLLLSLCLSAAA-------HDGAAVSQDLIRSSCLQASYPNLCVRTLSSYGGAVNTS-QD 68
           L+L  L LS AA        + ++   + I++SC   SYP+ C ++LSSY   +    + 
Sbjct: 9   LILFVLYLSTAAIVMARNLEEESSGDTEFIKASCETTSYPDRCFQSLSSYASEIKKQPRK 68

Query: 69  LAQAAISVSLSLARDLSRYLSDAR--RQASRRQRAAVNDCVDQIGDSVEELSNTLSVLRH 128
           LA+ A++VS++ A+    Y+S+    +  ++RQ  AV DC++++GD+V+ LSN+L  L+H
Sbjct: 69  LAETALAVSIARAKSAKTYVSEMTDYKGITKRQHEAVADCLEEMGDTVDRLSNSLKELKH 128

Query: 129 LPCGSDRRAFRLQMGNAKTWVSAALTNEYTCLDGF--KEVEGEVKLDVKKRISKVAKVTS 184
           L  G     F   + N +TW SAALT+E  C+DGF  K + GE+K  ++  I  VA+ TS
Sbjct: 129 LEEGDSGEDFWFCLSNVRTWTSAALTDETACMDGFGGKAMAGELKSLIRTHIVSVAEETS 188

[Close]

BLAST of Cla97C03G065030 vs. ExPASy Swiss-Prot
Match: P17407 (21 kDa protein OS=Daucus carota OX=4039 PE=2 SV=1)

HSP 1 Score: 108.2 bits (269), Expect = 1.1e-22
Identity = 65/181 (35.91%), Postives = 110/181 (60.77%), Query Frame = 0


Query: 10  LLLSLCLSAAAHDGAAVSQDLIRSSCLQASYPNLCVRTLSSYGGAV-NTSQDLAQAAISV 69
           L+ S  L   A   AA +   I++SC   +YP +C ++LS+Y   + N  Q+LA  A+ V
Sbjct: 8   LVFSALLVILAAASAAPANQFIKTSCTLTTYPAVCEQSLSAYAKTIQNNPQELASTALQV 67

Query: 70  SLSLARDLSRYLS--DARRQASRRQRAAVNDCVDQIGDSVEELSNTLSVLRHL--PCGSD 129
           SL+  +    ++   +  +    RQ AA++DC++++ DS++ +S +   +++L    G+D
Sbjct: 68  SLTRTQQAQTFMKRLNKFKGLKARQYAAIHDCLEEVEDSLDRVSRSCDEMKNLSHAKGND 127

Query: 130 RRAFRLQMGNAKTWVSAALTNEYTCLDGF--KEVEGEVKLDVKKRISKVAKVTSNALFFI 184
              F  +M N +TWVSAALT+E TC+DGF  K ++G++K  V+ ++  VA+VTSNAL  +
Sbjct: 128 ---FTFRMSNVETWVSAALTDETTCMDGFAGKGMDGKIKESVRAQVVAVARVTSNALALV 185

[Close]

BLAST of Cla97C03G065030 vs. ExPASy Swiss-Prot
Match: Q9STY5 (Pectinesterase inhibitor 11 OS=Arabidopsis thaliana OX=3702 GN=PMEI11 PE=2 SV=1)

HSP 1 Score: 102.1 bits (253), Expect = 7.7e-21
Identity = 72/193 (37.31%), Postives = 115/193 (59.59%), Query Frame = 0


Query: 3   FHSLRLLLLLSLCLSAAAH-DGAAVSQ----DLIRSSCLQASYPNLCVRTLSSYGGAVNT 62
           F++L L LL +  L+A++    AA++     + I++SC   +YP +CV +L+ Y  ++ T
Sbjct: 6   FYTLFLFLLSTAILTASSSAPRAAITSKRAINFIQASCKATTYPTVCVNSLTGYANSIQT 65

Query: 63  S-QDLAQAAISVSLSLARDLSRYLSDARRQAS--RRQRAAVNDCVDQIGDSVEELSNTLS 122
           S + LA+ A++V+++ A+    ++    R  S  +R+  AV DC+++I D+V+ L  T+S
Sbjct: 66  SPRRLAETALNVTVTQAQSTKVFVWRLGRFTSLKKREIQAVKDCIEEIHDAVDRL--TMS 125

Query: 123 VLRHLPCGS--DRRAFRLQMGNAKTWVSAALTNEYTCLDGF--KEVEGEVKLDVKKRISK 182
           +     CGS   R  F   M NA+TW SAALTN  TC DGF  + ++G VK  V+ RI  
Sbjct: 126 IHEVKMCGSAKGRDQFWFHMSNAQTWTSAALTNANTCSDGFAGRVMDGRVKNSVRARILN 185

Query: 183 VAKVTSNALFFIN 184
           + + TSNAL  IN
Sbjct: 186 LGRGTSNALALIN 196

[Close]

BLAST of Cla97C03G065030 vs. ExPASy TrEMBL
Match: A0A6J1H9I1 (pectinesterase inhibitor 3 OS=Cucurbita moschata OX=3662 GN=LOC111461775 PE=4 SV=1)

HSP 1 Score: 297.0 bits (759), Expect = 6.0e-77
Identity = 162/202 (80.20%), Postives = 176/202 (87.13%), Query Frame = 0


Query: 1   MTFHSLRLLLLLSLCLSAAAHDGAAVSQDLIRSSCLQASYPNLCVRTLSSYGGAVNTSQD 60
           MT  SL L+LLLSL LSAAAHDG   SQDLI SSCLQASYP+LC+RTLSSY GAVNT QD
Sbjct: 1   MTSLSLLLVLLLSLSLSAAAHDGPPPSQDLIHSSCLQASYPSLCIRTLSSYSGAVNTPQD 60

Query: 61  LAQAAISVSLSLARDLSRYLSDAR---RQASRRQRAAVNDCVDQIGDSVEELSNTLSVLR 120
           LAQA +SVSLSLARDLSRYLS++    RQAS+RQRAAV+DCV+QIGDSVEELSNTL +LR
Sbjct: 61  LAQATLSVSLSLARDLSRYLSNSSANLRQASKRQRAAVDDCVEQIGDSVEELSNTLGLLR 120

Query: 121 HLPCGSDRRAFRLQMGNAKTWVSAALTNEYTCLDGFKEVEGEVKLDVKKRISKVAKVTSN 180
           HLP G DRR+FRLQM NA+TWVSAALTNE TCLDGFKEV+GEVK DVKKRISKVAK TSN
Sbjct: 121 HLPYG-DRRSFRLQMSNAQTWVSAALTNEDTCLDGFKEVDGEVKFDVKKRISKVAKATSN 180

Query: 181 ALFFINRLDGGNPAGRKKLAAP 200
           AL+ INRLD G PAGRKKL AP
Sbjct: 181 ALYMINRLDVGTPAGRKKLTAP 201

[Close]

BLAST of Cla97C03G065030 vs. ExPASy TrEMBL
Match: A0A0A0LWR8 (PMEI domain-containing protein OS=Cucumis sativus OX=3659 GN=Csa_1G597770 PE=4 SV=1)

HSP 1 Score: 295.0 bits (754), Expect = 2.3e-76
Identity = 159/196 (81.12%), Postives = 170/196 (86.73%), Query Frame = 0


Query: 1   MTFHSLRLLLLLSLCLSAAAHDGAAVSQDLIRSSCLQASYPNLCVRTLSSYGGAVNTSQD 60
           MT  SL LLLL SL LSAA     A+SQDLI SSCLQASYP LC+RTLSSY GAV T +D
Sbjct: 28  MTVLSL-LLLLFSLSLSAAHGGRGAISQDLIHSSCLQASYPTLCIRTLSSYAGAVKTPRD 87

Query: 61  LAQAAISVSLSLARDLSRYLSDARRQASRRQRAAVNDCVDQIGDSVEELSNTLSVLRHLP 120
           LAQA ISVSLSLA++LS YLSD+ RQASR+QRAAV+DCVDQIGDSVEELSNTL VLRHLP
Sbjct: 88  LAQATISVSLSLAQNLSEYLSDSLRQASRQQRAAVDDCVDQIGDSVEELSNTLGVLRHLP 147

Query: 121 CGSDRRAFRLQMGNAKTWVSAALTNEYTCLDGFKEVEGEVKLDVKKRISKVAKVTSNALF 180
           CG DRR FRL+MGNAKTWVSAALTNE TCLDGFKEV+GEVKLDVK+RI KVAKVTSNALF
Sbjct: 148 CGDDRRKFRLEMGNAKTWVSAALTNEETCLDGFKEVDGEVKLDVKRRILKVAKVTSNALF 207

Query: 181 FINRLDGGNPAGRKKL 197
            INRLDGGNP GRK L
Sbjct: 208 MINRLDGGNPTGRKML 222

[Close]

BLAST of Cla97C03G065030 vs. ExPASy TrEMBL
Match: A0A6J1JAB3 (pectinesterase inhibitor 3 OS=Cucurbita maxima OX=3661 GN=LOC111484974 PE=4 SV=1)

HSP 1 Score: 294.7 bits (753), Expect = 3.0e-76
Identity = 161/202 (79.70%), Postives = 175/202 (86.63%), Query Frame = 0


Query: 1   MTFHSLRLLLLLSLCLSAAAHDGAAVSQDLIRSSCLQASYPNLCVRTLSSYGGAVNTSQD 60
           MT  SL L LLLSL LSAAAHDG   SQDLI SSCLQASYP+LC+RTLSSY GAVNT QD
Sbjct: 1   MTSLSLLLFLLLSLSLSAAAHDGPPPSQDLIHSSCLQASYPSLCIRTLSSYSGAVNTPQD 60

Query: 61  LAQAAISVSLSLARDLSRYLSDAR---RQASRRQRAAVNDCVDQIGDSVEELSNTLSVLR 120
           LAQA +SVSLSLARDLSRYLS++    RQAS+RQR AV+DCV+QIGDSVEELSNTL +LR
Sbjct: 61  LAQATLSVSLSLARDLSRYLSNSSVNLRQASKRQRVAVDDCVEQIGDSVEELSNTLGLLR 120

Query: 121 HLPCGSDRRAFRLQMGNAKTWVSAALTNEYTCLDGFKEVEGEVKLDVKKRISKVAKVTSN 180
           HLP G DRR+FRLQM NA+TWVSAALTNE TCLDGFKEV+GEVK DVKKRISKVAK TSN
Sbjct: 121 HLPYG-DRRSFRLQMSNAQTWVSAALTNEDTCLDGFKEVDGEVKFDVKKRISKVAKATSN 180

Query: 181 ALFFINRLDGGNPAGRKKLAAP 200
           AL+ INRL+ G PAGRKKLAAP
Sbjct: 181 ALYMINRLNVGTPAGRKKLAAP 201

[Close]

BLAST of Cla97C03G065030 vs. ExPASy TrEMBL
Match: A0A5A7UKZ9 (21 kDa protein OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold16G001300 PE=4 SV=1)

HSP 1 Score: 290.4 bits (742), Expect = 5.6e-75
Identity = 154/194 (79.38%), Postives = 168/194 (86.60%), Query Frame = 0


Query: 1   MTFHSLRLLLLLSLCLSAAAHDGAAVSQDLIRSSCLQASYPNLCVRTLSSYGGAVNTSQD 60
           MT  SL LLLLLSL LSAA   G AVSQDLIRSSCLQA YP LC+RTLSSY G+V T +D
Sbjct: 1   MTVLSLLLLLLLSLSLSAAHGGGGAVSQDLIRSSCLQARYPTLCIRTLSSYAGSVKTPRD 60

Query: 61  LAQAAISVSLSLARDLSRYLSDARRQASRRQRAAVNDCVDQIGDSVEELSNTLSVLRHLP 120
           LAQ  ISVSLSLA++LS YLSD+ R+ASR+QRAAV+DCVDQIGDSVEELSNTL VLRHLP
Sbjct: 61  LAQVTISVSLSLAQNLSEYLSDSLRKASRQQRAAVDDCVDQIGDSVEELSNTLGVLRHLP 120

Query: 121 CGSDRRAFRLQMGNAKTWVSAALTNEYTCLDGFKEVEGEVKLDVKKRISKVAKVTSNALF 180
           CG DRR FRL+MGNAKTWVSAALTNE TCLDGFKEV+GEVKLDVK+RI KVAKVTSNALF
Sbjct: 121 CGDDRRKFRLEMGNAKTWVSAALTNEETCLDGFKEVDGEVKLDVKRRIVKVAKVTSNALF 180

Query: 181 FINRLDGGNPAGRK 195
            INRLD GN  G++
Sbjct: 181 MINRLDSGNSTGKE 194

[Close]

BLAST of Cla97C03G065030 vs. ExPASy TrEMBL
Match: A0A1S3BR62 (21 kDa protein OS=Cucumis melo OX=3656 GN=LOC103492396 PE=4 SV=1)

HSP 1 Score: 290.4 bits (742), Expect = 5.6e-75
Identity = 154/194 (79.38%), Postives = 168/194 (86.60%), Query Frame = 0


Query: 1   MTFHSLRLLLLLSLCLSAAAHDGAAVSQDLIRSSCLQASYPNLCVRTLSSYGGAVNTSQD 60
           MT  SL LLLLLSL LSAA   G AVSQDLIRSSCLQA YP LC+RTLSSY G+V T +D
Sbjct: 1   MTVLSLLLLLLLSLSLSAAHGGGGAVSQDLIRSSCLQARYPTLCIRTLSSYAGSVKTPRD 60

Query: 61  LAQAAISVSLSLARDLSRYLSDARRQASRRQRAAVNDCVDQIGDSVEELSNTLSVLRHLP 120
           LAQ  ISVSLSLA++LS YLSD+ R+ASR+QRAAV+DCVDQIGDSVEELSNTL VLRHLP
Sbjct: 61  LAQVTISVSLSLAQNLSEYLSDSLRKASRQQRAAVDDCVDQIGDSVEELSNTLGVLRHLP 120

Query: 121 CGSDRRAFRLQMGNAKTWVSAALTNEYTCLDGFKEVEGEVKLDVKKRISKVAKVTSNALF 180
           CG DRR FRL+MGNAKTWVSAALTNE TCLDGFKEV+GEVKLDVK+RI KVAKVTSNALF
Sbjct: 121 CGDDRRKFRLEMGNAKTWVSAALTNEETCLDGFKEVDGEVKLDVKRRIVKVAKVTSNALF 180

Query: 181 FINRLDGGNPAGRK 195
            INRLD GN  G++
Sbjct: 181 MINRLDSGNSTGKE 194

[Close]

BLAST of Cla97C03G065030 vs. TAIR 10
Match: AT5G20740.1 (Plant invertase/pectin methylesterase inhibitor superfamily protein )

HSP 1 Score: 165.2 bits (417), Expect = 5.2e-41
Identity = 90/171 (52.63%), Postives = 126/171 (73.68%), Query Frame = 0


Query: 21  HDGAAVSQDLIRSSCLQASYPNLCVRTLSSYGG-AVNTSQDLAQAAISVSLSLARDLSRY 80
           H+GA   +D++ SSC  ASYP+LCVRTLSSY G  +   +DLAQAAI +SLS A+  ++ 
Sbjct: 27  HNGA---EDIVHSSCEHASYPSLCVRTLSSYSGPTITNRRDLAQAAIKISLSHAQSAAKK 86

Query: 81  LSDARRQASRR--QRAAVNDCVDQIGDSVEELSNTLSVLRHL-PCGSDRRAFRLQMGNAK 140
           L+  R    ++  ++AA+ DCV+ IGDSV+ELS TL VL+HL   G   + FR QM NA+
Sbjct: 87  LAVVRDSVGKKKQEKAALVDCVEMIGDSVDELSRTLGVLKHLRVSGGSAKEFRWQMSNAQ 146

Query: 141 TWVSAALTNEYTCLDGFKEV-EGEVKLDVKKRISKVAKVTSNALFFINRLD 187
           TW SAALT++ TCLDGF+ + +GE+K +VK+ ++KVA+VTSNAL+ +N+LD
Sbjct: 147 TWASAALTDDDTCLDGFQGMDDGEIKTEVKQWMTKVARVTSNALYMVNQLD 194

[Close]

BLAST of Cla97C03G065030 vs. TAIR 10
Match: AT1G62770.1 (Plant invertase/pectin methylesterase inhibitor superfamily protein )

HSP 1 Score: 118.2 bits (295), Expect = 7.3e-27
Identity = 70/186 (37.63%), Postives = 113/186 (60.75%), Query Frame = 0


Query: 6   LRLLLLLSLCLSAAAHDGAAVSQDLIRSSCLQASYPNLCVRTLSSYGGAV--NTSQDLAQ 65
           L +LL +++  S++A    + S   I SSC    YP+LCV TLS+Y   +  N  QDLAQ
Sbjct: 9   LVILLSITILQSSSATPNRSESDQFIVSSCQTTQYPSLCVHTLSAYATKIRHNNDQDLAQ 68

Query: 66  AAISVSLSLARDLSRYLSDARRQA---SRRQRAAVNDCVDQIGDSVEELSNTLSVLRHLP 125
            A+ +SL+ A+ ++ +++   ++     RR+  A+ DC++ +G+SV+ L+ ++  L    
Sbjct: 69  TALIISLARAKSVTIFVAKLTKETPKFKRREYLAIKDCIEVLGNSVDRLAQSVKELARAG 128

Query: 126 CGSDRRAFRLQMGNAKTWVSAALTNEYTCLDGFKE--VEGEVKLDVKKRISKVAKVTSNA 185
                  F  +M N +TWVSAALT+E TCLDGF E  + G+VK  ++ ++  VA+VTSNA
Sbjct: 129 HAVASEDFMWKMSNVQTWVSAALTDETTCLDGFSERAMGGKVKRLIRYKVVHVAQVTSNA 188

[Close]

BLAST of Cla97C03G065030 vs. TAIR 10
Match: AT4G25260.1 (Plant invertase/pectin methylesterase inhibitor superfamily protein )

HSP 1 Score: 115.9 bits (289), Expect = 3.6e-26
Identity = 71/187 (37.97%), Postives = 111/187 (59.36%), Query Frame = 0


Query: 9   LLLLSLCLSAAA-------HDGAAVSQDLIRSSCLQASYPNLCVRTLSSYGGAVNTS-QD 68
           L+L  L LS AA        + ++   + I++SC   SYP+ C ++LSSY   +    + 
Sbjct: 9   LILFVLYLSTAAIVMARNLEEESSGDTEFIKASCETTSYPDRCFQSLSSYASEIKKQPRK 68

Query: 69  LAQAAISVSLSLARDLSRYLSDAR--RQASRRQRAAVNDCVDQIGDSVEELSNTLSVLRH 128
           LA+ A++VS++ A+    Y+S+    +  ++RQ  AV DC++++GD+V+ LSN+L  L+H
Sbjct: 69  LAETALAVSIARAKSAKTYVSEMTDYKGITKRQHEAVADCLEEMGDTVDRLSNSLKELKH 128

Query: 129 LPCGSDRRAFRLQMGNAKTWVSAALTNEYTCLDGF--KEVEGEVKLDVKKRISKVAKVTS 184
           L  G     F   + N +TW SAALT+E  C+DGF  K + GE+K  ++  I  VA+ TS
Sbjct: 129 LEEGDSGEDFWFCLSNVRTWTSAALTDETACMDGFGGKAMAGELKSLIRTHIVSVAEETS 188

[Close]

BLAST of Cla97C03G065030 vs. TAIR 10
Match: AT1G23205.1 (Plant invertase/pectin methylesterase inhibitor superfamily protein )

HSP 1 Score: 114.0 bits (284), Expect = 1.4e-25
Identity = 75/195 (38.46%), Postives = 110/195 (56.41%), Query Frame = 0


Query: 1   MTFHSLRLLLLLSLCLSAAAHDGAAVSQDLIRSSCLQASYPNLCVRTLSSYGGAV-NTSQ 60
           + F  L  +L L L + + + D      D IR+SC    YP+LC  +LSS+  +V N   
Sbjct: 9   LLFFFLASVLPLILTVHSQSDD-----SDFIRTSCNTTLYPDLCFSSLSSFSSSVHNDPA 68

Query: 61  DLAQAAISVSLSLARDLSRYLS-------DARRQASRRQRAAV--NDCVDQIGDSVEELS 120
            LA+AAISV+L+   DL+ YL+       ++    +    AA   +DC D + D+VEE+ 
Sbjct: 69  LLARAAISVTLTKTLDLASYLANITTLQPESNEDGAHHPTAAAVFHDCFDNLKDAVEEMK 128

Query: 121 NTLSVLRHLPCGSDRRAFRLQMGNAKTWVSAALTNEYTCLDGFKEVEGEV-KLDVKKRIS 180
            ++  +R L       +FR QM N +TW+SAALT+E TC DGFK++  E  K D+  R+ 
Sbjct: 129 GSMKQMRELVSTGSLESFRFQMSNVQTWLSAALTDEETCTDGFKDIHDEPRKDDICARVD 188

Query: 181 KVAKVTSNALFFINR 185
            V K+TSNAL  +NR
Sbjct: 189 DVKKMTSNALALVNR 198

[Close]

BLAST of Cla97C03G065030 vs. TAIR 10
Match: AT5G62350.1 (Plant invertase/pectin methylesterase inhibitor superfamily protein )

HSP 1 Score: 112.1 bits (279), Expect = 5.3e-25
Identity = 72/186 (38.71%), Postives = 107/186 (57.53%), Query Frame = 0


Query: 5   SLRLLLLLSLCLSAAAHDGAAVSQ--DLIRSSCLQASYPNLCVRTLSSYGGAVNTS-QDL 64
           S+  LL L L  + AA    A  +  + I+SSC   +YP LCV +LS Y   + TS + L
Sbjct: 11  SISYLLSLELTAATAASQTGASKKAINFIQSSCKTTTYPALCVHSLSVYANDIQTSPKRL 70

Query: 65  AQAAISVSLSLARDLSRYLSDARRQ--ASRRQRAAVNDCVDQIGDSVEELSNTLSVLRHL 124
           A+ AI+V+LS A+    ++S   R     +R+  A+ DCV+++ D+V+ L+ ++  L+  
Sbjct: 71  AETAIAVTLSRAQSTKLFVSRLTRMKGLKKREVEAIKDCVEEMNDTVDRLTKSVQELKLC 130

Query: 125 PCGSDRRAFRLQMGNAKTWVSAALTNEYTCLDGF--KEVEGEVKLDVKKRISKVAKVTSN 184
               D+  F   M NA+TW SAALT+E TC DGF  + ++G +K  V+ RI  V   TSN
Sbjct: 131 GSAKDQDQFAYHMSNAQTWTSAALTDENTCSDGFSGRVMDGRIKNSVRARIMNVGHETSN 190

The following BLAST results are available for this feature:

BLAST of Cla97C03G065030 vs. NCBI nr


Match Name	E-value	Identity	Description
XP_038879670.1	1.9e-85	89.45	pectinesterase inhibitor 3 [Benincasa hispida]	[more]
XP_023516503.1	5.6e-77	80.69	pectinesterase inhibitor 3 [Cucurbita pepo subsp. pepo]	[more]
XP_022961187.1	1.2e-76	80.20	pectinesterase inhibitor 3 [Cucurbita moschata] >KAG6590055.1 Pectinesterase inh...	[more]
XP_004144132.2	4.7e-76	81.12	pectinesterase inhibitor 3 [Cucumis sativus] >KGN66330.1 hypothetical protein Cs...	[more]
XP_022987422.1	6.1e-76	79.70	pectinesterase inhibitor 3 [Cucurbita maxima]	[more]

BLAST of Cla97C03G065030 vs. ExPASy Swiss-Prot


Match Name	E-value	Identity	Description
Q84WE4	7.4e-40	52.63	Pectinesterase inhibitor 3 OS=Arabidopsis thaliana OX=3702 GN=PMEI3 PE=2 SV=1	[more]
Q9SI72	1.0e-25	37.63	Pectinesterase inhibitor 9 OS=Arabidopsis thaliana OX=3702 GN=PMEI9 PE=2 SV=1	[more]
Q9SB37	5.1e-25	37.97	Pectinesterase inhibitor 7 OS=Arabidopsis thaliana OX=3702 GN=PMEI7 PE=2 SV=1	[more]
P17407	1.1e-22	35.91	21 kDa protein OS=Daucus carota OX=4039 PE=2 SV=1	[more]
Q9STY5	7.7e-21	37.31	Pectinesterase inhibitor 11 OS=Arabidopsis thaliana OX=3702 GN=PMEI11 PE=2 SV=1	[more]

BLAST of Cla97C03G065030 vs. ExPASy TrEMBL


Match Name	E-value	Identity	Description
A0A6J1H9I1	6.0e-77	80.20	pectinesterase inhibitor 3 OS=Cucurbita moschata OX=3662 GN=LOC111461775 PE=4 SV...	[more]
A0A0A0LWR8	2.3e-76	81.12	PMEI domain-containing protein OS=Cucumis sativus OX=3659 GN=Csa_1G597770 PE=4 S...	[more]
A0A6J1JAB3	3.0e-76	79.70	pectinesterase inhibitor 3 OS=Cucurbita maxima OX=3661 GN=LOC111484974 PE=4 SV=1	[more]
A0A5A7UKZ9	5.6e-75	79.38	21 kDa protein OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold16G001300...	[more]
A0A1S3BR62	5.6e-75	79.38	21 kDa protein OS=Cucumis melo OX=3656 GN=LOC103492396 PE=4 SV=1	[more]

BLAST of Cla97C03G065030 vs. TAIR 10


Match Name	E-value	Identity	Description
AT5G20740.1	5.2e-41	52.63	Plant invertase/pectin methylesterase inhibitor superfamily protein	[more]
AT1G62770.1	7.3e-27	37.63	Plant invertase/pectin methylesterase inhibitor superfamily protein	[more]
AT4G25260.1	3.6e-26	37.97	Plant invertase/pectin methylesterase inhibitor superfamily protein	[more]
AT1G23205.1	1.4e-25	38.46	Plant invertase/pectin methylesterase inhibitor superfamily protein	[more]
AT5G62350.1	5.3e-25	38.71	Plant invertase/pectin methylesterase inhibitor superfamily protein	[more]

InterPro

Analysis Name: InterPro Annotations of Watermelon (97103) v2.5
Date Performed: 2022-01-31


IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
IPR006501	Pectinesterase inhibitor domain	SMART	SM00856	PMEI_2	coord: 26..181 e-value: 3.7E-41 score: 152.7
IPR006501	Pectinesterase inhibitor domain	PFAM	PF04043	PMEI	coord: 29..179 e-value: 9.6E-35 score: 120.2
IPR006501	Pectinesterase inhibitor domain	TIGRFAM	TIGR01614	TIGR01614	coord: 6..185 e-value: 1.2E-33 score: 113.9
IPR035513	Invertase/pectin methylesterase inhibitor domain superfamily	GENE3D	1.20.140.40	Invertase/pectin methylesterase inhibitor family protein	coord: 24..186 e-value: 7.3E-37 score: 128.7
IPR035513	Invertase/pectin methylesterase inhibitor domain superfamily	SUPERFAMILY	101148	Plant invertase/pectin methylesterase inhibitor	coord: 28..185
None	No IPR available	PANTHER	PTHR31080:SF110	PECTINESTERASE INHIBITOR 3	coord: 3..187
None	No IPR available	PANTHER	PTHR31080	PECTINESTERASE INHIBITOR-LIKE	coord: 3..187
None	No IPR available	CDD	cd15798	PMEI-like_3	coord: 33..187 e-value: 2.49568E-36 score: 122.547

Relationships

The following mRNA feature(s) are a part of this gene:


Feature Name	Unique Name	Type
Cla97C03G065030.1	Cla97C03G065030.1	mRNA

GO Annotation

GO Assignments

This gene is annotated with the following GO terms.

Category	Term Accession	Term Name
biological_process	GO:0043086	negative regulation of catalytic activity
molecular_function	GO:0004857	enzyme inhibitor activity