Cla97C03G064220 (gene) Watermelon (97103) v2.5

Overview


Name	Cla97C03G064220
Type	gene
Organism	Citrullus lanatus subsp. vulgaris cv. 97103 (Watermelon (97103) v2.5)
Description	L-ascorbate oxidase
Location	Cla97Chr03: 27686012 .. 27690141 (+)
RNA-Seq Expression	Cla97C03G064220
Synteny	Cla97C03G064220

Sequences

The following sequences are available for this feature:

Gene sequence (with intron)

Legend: exonCDSpolypeptide

Hold the cursor over a type above to highlight its positions in the sequence below.

ATGCCTTTCCTAGTCATCTTCTTGGTTTTGTCAATCATGTCTGGTTTTGGAATAACACTCTCTGAGGCAGTTCCCAAAGTCAGACACTACAAATGGGAAGTTGAGTACATGTTTTGGTACCCCGACTGTGTCGAAAACATCGTCATGGGAATCAACGGTCAGTTTCCCGGGCCAACGATTAGAGCCAACGTTGGTGACACCATCGTTGTAGAGATGATTAACAAACTCTCCACTGAAGGCCTCGTTATTCACTGGCATGGAATCCTACAACGAGGAACTCCCTGGGCCGATGGCACTGCTTCCATCTCACAGTGTGCCACTAACCCAGGTGAAACCTTCATTTACCGGTTCGTAGTGGATAAGGTCAGAAGGATTTAAAATATCAATGTCAATGAAAGTGCCATATTGTGTCTAATAAAATTTATTCGTTTATTGAACTTTTTTTTTCGTAGGCTGGGACATATTTTTATCATGGCCATTTAGGGATGCAAAGAGCAGCAGGGTTGTATGGATCCTTGATAGTGGATCTAGAAGAAGGAAAGAAAGAGCCCTTCCATTATGACGAAGATATCACCTTATTGCTTAGTGATTGGTGGCATAAAACTGTTCAAAAGCAAGAAGTTGGCCTCAACTCCAACCCTATGCATTGGATTAATGAGCCCCAGGTAGTTAATTTATGTCTTATCTACTAAATTATGTTTGAGTTAACTCTCAAATATTATATATTTAATTAAATTATGCGATTCAACTCTGAAAAGTTTCATTTTTTATTCTAATATATATTCTTGAAATTTCTTACATATAAACACACATCTTTGAGAGTTTTTTTTTTTTTTTTTTTTTTTGTCCTATTAAAAAAGATGAAGGTGGCATAAAGGTTAAGGTTTAAAATTTGAAAGACCTACTTTAACAAGAAAAAAAAAAAAGTTATTGAACAATTTCAATATTTTAGGATATTTTTTTTTAAACAAAGTTTAAAATTTATGCATAAAAACAAAACATCTCAAATCAAAAGAGATAATTGAAACATATATGTCTTTACTTAAATTCTAAAGCAAAAACTTTATTTTAAAAATATTTATGAGTGTTCGAACTAGCTTACATGTACCAACTAATTTTACGGAACAATTTATCTAGCCCTAAAATATTTGTGTGTCAAGGAAAACAAATATTTTCAAAATTAATTTTTAAAGAATTTGTATTAGAAAAAAAAAAAAAAACTACTTTTTGAATTTTTTTTTTAGTTTTCAAAATTTAAACTCTTTTTTAAAAAATTGCTAGATCGTGAATAGCACAAAACAAAGAAGCTTATAAGTGAAAATAATATTACAAATTTATTTTTTTTAAAAAAATAAATAAAGGTCACATTTGATAATTATTTAATTTAACTTTTTTTTACAATATCAAGTTCATTTCCTCTCAATTTAATATTATAAAAACAAAAACTAGTTTTTTTTACTTTTCAAAACTTTACTTGTTTTTAGAAAATACATGTAAAAGGTATATAAAGAAAAAACAAGAAATTTAGAAGGGAAAGAGAGATCTGTAAAGCTTAATATTCAAAAATTAAAAACAAAAAACTAAGAGCCCGTTTGGCAATCATTTCCTTTCTTGCTTTTTATCTTTTAAAATTAAGCTTATTTCCTCCTAATTTTTTATCATGATTTGCATATTTCTTAAGTACAAGAATTGAATTCTTAGACAAATTCTAAAAATAAAAACAAGCTTTTTAAAAAAAAATACTTTTTTAAAAAATTGGCTTGATTTTTGTAAACATGGGTAAAAAAATTGATTAAAAAAAATAAAAAAGTAAAATAAGTATTTATAAACTTAATTTTTAAAAATTAAGAAAAAAAACTAAATAACTATAAAATACGGCCTAAACAGTTATGTAAATGATTATCACCTTAGATTTAAATTGCTTTCTTTCTTTGGACATGAGAACATATTGACAGATTCCTTTTTTTTTTCAATATTGAAAACGTTTGCTGCTTTTTCCCGAGCAAAAACAAATAAACAAAACAAAAAAAAATATAAAATAAAATAAAAAAATAAACAAAATAAAAAATAAACAAAACAAAAAAACAAAAAAAACAAAAAAAGAAAAAGAAAAACTGTCCACATAATATTGTCTCTCCCCTCTCTAATCTTTCCCCGTTTCTGTCCTTGAATTGTATGTGAGAGAGTTTTATTTTTATTTTATTTTATTTTTTCTCTCTTTTTTTATCCTTTAAACTTCATTAAACCAAACAAAACTAATTACAATCAAAGTTCTTTATTGAAAATAGAGGACACTCAACTAGAATAATGCGGTAGAAGAAAACTTTTTAAAGTAAATTTGTATATAATATAAGAAAATTTAAACTTTCAATTTTATGTAAAATATATTTTTAAATTTTAAAAAATGTCGAATAAGTAAAAAACATATTAAAACATAGAATTAAAAGTTCAACTACTTATTAGAAATTTCTAAAGTTTAAAAATCTATTAGACTCAAACTTTAACAATTCGTGAATAATATTAAGCATAATTCAATTAGAAATATCATCAAACAAGAGTTCAAATGTTCCAATATTTTTTTTCTTACATGTCGGGTGAACTCTAAAAATAAATAAATATGATAGAGAAAAGGAATTTTTTTTTTTTTTAAGCCGGCAGTGTATCCAAAATTTGAATTTTATATTAACTTTTAGGGTTAACTTTTAGCATTAACACTAATGTAGGGAAAATCCATATGGGACACATTTATTGAAATTTAGATAAAATGACATAATTTAAAATTGATTGGAAAAAAAATAATAAAAAAGTTGATTGAAAAAAATTGAGAGATTGGTGGGGCATGAAAATTATCCCAACCCAATCAAAATCCTCAAAGATTATCAAATTTAATCCAATTTATATCCCACAATTAATTAAAATCAACATTAATGATTCATTGATATTTATTTATTAATTAATTAGCCTATATCTTATTGTTGATTTTCCAGGTATAAAAACTACACCTATATGGTCAGCATTAGAAAACCTATATTAGATGGGTTTTAAAGTTATCTAAATAATAATAATAATAACAATAATATAATTGGTTTTAAAAATAAAATCTTATGAAATAAACAAGTATATTTTAATGTTTGTTTGAAATCCTATATCAAAATATGTGAAACAAAATAGACCCTTAGGACAAAAATTCTAAAATATTGGATAAAGAAAATGACTTAAATTGAGACTTGAACATCGTCAAACTGTTCTAAGTTACCAATATACGCATGCAGTCAACTAGTTTCAATTTGCATCCAAAACCTATATGATTTTATTTCAAAGCTAATTTTGAAACATGTATAAAAAGGTCGAAAATAATATTAGTGTTTGGTCAATTTAGGTAAAGTATTTATTAGAGATTTAATAATTGAACTATAGTGTTGTTGATTAATTAAATAGAATGTTTTTATAATATTTATGTTGTTGTTGTTGTTGTTGAAGGCCATACTGATAAATGGGAGAGGGCAGACCAATTGTTCAATAGCAGCAAAATACAACACAAGTTTGAAGCAATGTGAGTTGAAAGGAGATGAACAATGTGCACCATTTATTCTTCATGTCAAACCCAATAAGACTTATAGGATAAGAATTGCAAGTACCACTTCATTGTCTGCCCTCAACTTTGCCATTCGGGTAACTCTCTCTCTCTCTTAAATGTTAAAATTGCTAAATAGTGATATTTTGTTATATTTATACAAATTTGGTTCTTTTGCTATATTTGATAATAACGTTGTTATTCTTGATTTTTAATATTTTTTTTAATCATTTTTCTTCTTCAGAATCATCAACTGTTAGTGGTAGAAGCCGACGGTAACTACGTTCAACCATTTCTAACTTCCGACATCGACATTTATTCCGGCGAGTCCTACTCCGTCCTAATCACCACCGATCAACCGGCATCAGAGAATTACTGGGTTTCCGTCGGCGTTCCGCGCGCGGCCACCCAAAACGCCTCCGGCACTAACTATCCTCAACTACCTCCCCAACTCCGTCTCCAAATTACCCATTTCTCCGCCCCCGGAAATCCCCAATTGGGACGATTACGACCGCAGCAAAAACTTCACATACAGAATCTTAGCCGCCATGGGTAG

mRNA sequence

ATGCCTTTCCTAGTCATCTTCTTGGTTTTGTCAATCATGTCTGGTTTTGGAATAACACTCTCTGAGGCAGTTCCCAAAGTCAGACACTACAAATGGGAAGTTGAGTACATGTTTTGGTACCCCGACTGTGTCGAAAACATCGTCATGGGAATCAACGGTCAGTTTCCCGGGCCAACGATTAGAGCCAACGTTGGTGACACCATCGTTGTAGAGATGATTAACAAACTCTCCACTGAAGGCCTCGTTATTCACTGGCATGGAATCCTACAACGAGGAACTCCCTGGGCCGATGGCACTGCTTCCATCTCACAGTGTGCCACTAACCCAGGTGAAACCTTCATTTACCGGTTCGTAGTGGATAAGGCTGGGACATATTTTTATCATGGCCATTTAGGGATGCAAAGAGCAGCAGGGTTGTATGGATCCTTGATAGTGGATCTAGAAGAAGGAAAGAAAGAGCCCTTCCATTATGACGAAGATATCACCTTATTGCTTAGTGATTGGTGGCATAAAACTGTTCAAAAGCAAGAAGTTGGCCTCAACTCCAACCCTATGCATTGGATTAATGAGCCCCAGGCCATACTGATAAATGGGAGAGGGCAGACCAATTGTTCAATAGCAGCAAAATACAACACAAGTTTGAAGCAATGTGAGTTGAAAGGAGATGAACAATGTGCACCATTTATTCTTCATGTCAAACCCAATAAGACTTATAGGATAAGAATTGCAAGTACCACTTCATTGTCTGCCCTCAACTTTGCCATTCGGAATCATCAACTGTTAGTGGTAGAAGCCGACGGTAACTACGTTCAACCATTTCTAACTTCCGACATCGACATTTATTCCGGCGAGTCCTACTCCGTCCTAATCACCACCGATCAACCGGCATCAGAGAATTACTGGGTTTCCGTCGGCGTTCCGCGCGCGGCCACCCAAAACGCCTCCGGCACTAACTATCCTCAACTACCTCCCCAACTCCGTCTCCAAATTACCCATTTCTCCGCCCCCGGAAATCCCCAATTGGGACGATTACGACCGCAGCAAAAACTTCACATACAGAATCTTAGCCGCCATGGGTAG

Coding sequence (CDS)

ATGCCTTTCCTAGTCATCTTCTTGGTTTTGTCAATCATGTCTGGTTTTGGAATAACACTCTCTGAGGCAGTTCCCAAAGTCAGACACTACAAATGGGAAGTTGAGTACATGTTTTGGTACCCCGACTGTGTCGAAAACATCGTCATGGGAATCAACGGTCAGTTTCCCGGGCCAACGATTAGAGCCAACGTTGGTGACACCATCGTTGTAGAGATGATTAACAAACTCTCCACTGAAGGCCTCGTTATTCACTGGCATGGAATCCTACAACGAGGAACTCCCTGGGCCGATGGCACTGCTTCCATCTCACAGTGTGCCACTAACCCAGGTGAAACCTTCATTTACCGGTTCGTAGTGGATAAGGCTGGGACATATTTTTATCATGGCCATTTAGGGATGCAAAGAGCAGCAGGGTTGTATGGATCCTTGATAGTGGATCTAGAAGAAGGAAAGAAAGAGCCCTTCCATTATGACGAAGATATCACCTTATTGCTTAGTGATTGGTGGCATAAAACTGTTCAAAAGCAAGAAGTTGGCCTCAACTCCAACCCTATGCATTGGATTAATGAGCCCCAGGCCATACTGATAAATGGGAGAGGGCAGACCAATTGTTCAATAGCAGCAAAATACAACACAAGTTTGAAGCAATGTGAGTTGAAAGGAGATGAACAATGTGCACCATTTATTCTTCATGTCAAACCCAATAAGACTTATAGGATAAGAATTGCAAGTACCACTTCATTGTCTGCCCTCAACTTTGCCATTCGGAATCATCAACTGTTAGTGGTAGAAGCCGACGGTAACTACGTTCAACCATTTCTAACTTCCGACATCGACATTTATTCCGGCGAGTCCTACTCCGTCCTAATCACCACCGATCAACCGGCATCAGAGAATTACTGGGTTTCCGTCGGCGTTCCGCGCGCGGCCACCCAAAACGCCTCCGGCACTAACTATCCTCAACTACCTCCCCAACTCCGTCTCCAAATTACCCATTTCTCCGCCCCCGGAAATCCCCAATTGGGACGATTACGACCGCAGCAAAAACTTCACATACAGAATCTTAGCCGCCATGGGTAG

Protein sequence

MPFLVIFLVLSIMSGFGITLSEAVPKVRHYKWEVEYMFWYPDCVENIVMGINGQFPGPTIRANVGDTIVVEMINKLSTEGLVIHWHGILQRGTPWADGTASISQCATNPGETFIYRFVVDKAGTYFYHGHLGMQRAAGLYGSLIVDLEEGKKEPFHYDEDITLLLSDWWHKTVQKQEVGLNSNPMHWINEPQAILINGRGQTNCSIAAKYNTSLKQCELKGDEQCAPFILHVKPNKTYRIRIASTTSLSALNFAIRNHQLLVVEADGNYVQPFLTSDIDIYSGESYSVLITTDQPASENYWVSVGVPRAATQNASGTNYPQLPPQLRLQITHFSAPGNPQLGRLRPQQKLHIQNLSRHG

Homology

[Close]

BLAST of Cla97C03G064220 vs. NCBI nr
Match: XP_038878978.1 (L-ascorbate oxidase [Benincasa hispida])

HSP 1 Score: 552.7 bits (1423), Expect = 2.3e-153
Identity = 264/328 (80.49%), Postives = 289/328 (88.11%), Query Frame = 0


Query: 2   PFLVIFLVLSIMSGFGITLSEA-VPKVRHYKWEVEYMFWYPDCVENIVMGINGQFPGPTI 61
           PFL IF +LSI+ GFGITLSEA  PK+RHYKWEVEYMFW PDCVENIVMGI+GQFPGPTI
Sbjct: 13  PFL-IFFILSIIFGFGITLSEAGFPKIRHYKWEVEYMFWSPDCVENIVMGIDGQFPGPTI 72

Query: 62  RANVGDTIVVEMINKLSTEGLVIHWHGILQRGTPWADGTASISQCATNPGETFIYRFVVD 121
           RANVGDT+VVE+ NKL TEG+VIHWHGI+QRGTPWADGTASISQCA NPGETF YRFVVD
Sbjct: 73  RANVGDTVVVELTNKLHTEGVVIHWHGIMQRGTPWADGTASISQCAINPGETFTYRFVVD 132

Query: 122 KAGTYFYHGHLGMQRAAGLYGSLIVDLEEGKKEPFHYDEDITLLLSDWWHKTVQKQEVGL 181
           KAGTYFYHGHLGMQR+AGLYGSLIVD EEGK EPFHYDE+I LLLSDWWH++V KQEVGL
Sbjct: 133 KAGTYFYHGHLGMQRSAGLYGSLIVDPEEGKNEPFHYDEEINLLLSDWWHQSVHKQEVGL 192

Query: 182 NSNPMHWINEPQAILINGRGQTNCSIAAKYNTSLKQCELKGDEQCAPFILHVKPNKTYRI 241
           +S P+ WI EPQ ILINGRGQ +CSIAAKYN +LKQCELKG EQCAPFILHV PNKTYRI
Sbjct: 193 SSKPIRWIGEPQTILINGRGQFDCSIAAKYNNNLKQCELKGGEQCAPFILHVLPNKTYRI 252

Query: 242 RIASTTSLSALNFAIRNHQLLVVEADGNYVQPFLTSDIDIYSGESYSVLITTDQPASENY 301
           RIASTT+L++LNFAI NH+LLVVEADGNY+QPFLTSDIDIYSGESYSVLITTDQP+SENY
Sbjct: 253 RIASTTALASLNFAIGNHRLLVVEADGNYIQPFLTSDIDIYSGESYSVLITTDQPSSENY 312

Query: 302 WVSVGVPRAATQNASGTNYPQLPPQLRL 329
           WVS+GV             P+ PP L +
Sbjct: 313 WVSIGV---------RARLPKTPPGLTI 330

[Close]

BLAST of Cla97C03G064220 vs. NCBI nr
Match: XP_038880052.1 (L-ascorbate oxidase-like [Benincasa hispida])

HSP 1 Score: 540.8 bits (1392), Expect = 8.9e-150
Identity = 256/328 (78.05%), Postives = 285/328 (86.89%), Query Frame = 0


Query: 1   MPFLVIFLVLSIMSGFGITLSEAVPKVRHYKWEVEYMFWYPDCVENIVMGINGQFPGPTI 60
           +PFLVIFL+ S++SGFGITLSEAV   R++KWEVEYMFW PDCVEN+VMGINGQFPGPTI
Sbjct: 12  LPFLVIFLIFSMISGFGITLSEAVNNTRYFKWEVEYMFWSPDCVENLVMGINGQFPGPTI 71

Query: 61  RANVGDTIVVEMINKLSTEGLVIHWHGILQRGTPWADGTASISQCATNPGETFIYRFVVD 120
           RANVGDTI+VE++NKL TEG+VIHWHGILQR TPWADGTASISQCA NPGETFIY+F  D
Sbjct: 72  RANVGDTIIVELVNKLFTEGVVIHWHGILQRETPWADGTASISQCAINPGETFIYQFKAD 131

Query: 121 KAGTYFYHGHLGMQRAAGLYGSLIVDLEEGKKEPFHYDEDITLLLSDWWHKTVQKQEVGL 180
           K GTYFYHGHLGMQR+AGLYGSLI+D EEGKKEPFHYDE+I LLLSDWWHK+  KQEVGL
Sbjct: 132 KPGTYFYHGHLGMQRSAGLYGSLIIDPEEGKKEPFHYDEEINLLLSDWWHKSAHKQEVGL 191

Query: 181 NSNPMHWINEPQAILINGRGQTNCSIAAKYNTSLKQCELKGDEQCAPFILHVKPNKTYRI 240
           +S+PM WI EP+ ILINGRGQ +CSIAAKYN +L+QC+LKG EQCAPFIL VKP KTYRI
Sbjct: 192 SSSPMRWIGEPETILINGRGQISCSIAAKYNKNLEQCKLKGGEQCAPFILQVKPKKTYRI 251

Query: 241 RIASTTSLSALNFAIRNHQLLVVEADGNYVQPFLTSDIDIYSGESYSVLITTDQPASENY 300
           RIASTTSLSALN AI NHQLLVVEADGNYV+PF+TSDIDIYSGESYSVLITT+Q  ++NY
Sbjct: 252 RIASTTSLSALNLAIGNHQLLVVEADGNYVEPFVTSDIDIYSGESYSVLITTNQDPAQNY 311

Query: 301 WVSVGVPRAATQNASGTNYPQLPPQLRL 329
           WVSVGV             P+ PP L L
Sbjct: 312 WVSVGV---------RGRLPKTPPALTL 330

[Close]

BLAST of Cla97C03G064220 vs. NCBI nr
Match: KAA0055636.1 (L-ascorbate oxidase [Cucumis melo var. makuwa])

HSP 1 Score: 538.1 bits (1385), Expect = 5.7e-149
Identity = 256/306 (83.66%), Postives = 274/306 (89.54%), Query Frame = 0


Query: 2   PFLVIFLVLSIMSGFGITLSEA-VPKVRHYKWEVEYMFWYPDCVENIVMGINGQFPGPTI 61
           PFLV F VLSI+ GFGITLSEA  PK+RHYKWEVEYMFW PDCVENIVMGINGQFPGPTI
Sbjct: 13  PFLV-FFVLSIIFGFGITLSEAGFPKIRHYKWEVEYMFWSPDCVENIVMGINGQFPGPTI 72

Query: 62  RANVGDTIVVEMINKLSTEGLVIHWHGILQRGTPWADGTASISQCATNPGETFIYRFVVD 121
           RAN GD +VVE+ NKL TEG+VIHWHGILQRGTPWADGTASISQCA NPGETF YRFVVD
Sbjct: 73  RANAGDMVVVELTNKLHTEGVVIHWHGILQRGTPWADGTASISQCAINPGETFTYRFVVD 132

Query: 122 KAGTYFYHGHLGMQRAAGLYGSLIVDLEEGKKEPFHYDEDITLLLSDWWHKTVQKQEVGL 181
           KAGTYFYHGHLGMQR+AGLYGSLIVD +EG  EPFHYDE+I LLLSDWWH++V KQEVGL
Sbjct: 133 KAGTYFYHGHLGMQRSAGLYGSLIVDPQEGTSEPFHYDEEINLLLSDWWHQSVHKQEVGL 192

Query: 182 NSNPMHWINEPQAILINGRGQTNCSIAAKYNTSLKQCELKGDEQCAPFILHVKPNKTYRI 241
           +S PM WI EPQ+ILINGRGQ +CSIAAKY   LKQCEL G EQCAPFILHV P KTYRI
Sbjct: 193 SSKPMRWIGEPQSILINGRGQFDCSIAAKYKNGLKQCELSGKEQCAPFILHVHPKKTYRI 252

Query: 242 RIASTTSLSALNFAIRNHQLLVVEADGNYVQPFLTSDIDIYSGESYSVLITTDQPASENY 301
           RIASTT+L++LNFAI NH+LLVVEADGNYVQPF TSDIDIYSGESYSVLITTDQ  SENY
Sbjct: 253 RIASTTALASLNFAIGNHKLLVVEADGNYVQPFSTSDIDIYSGESYSVLITTDQNPSENY 312

Query: 302 WVSVGV 307
           WVS+GV
Sbjct: 313 WVSIGV 317

[Close]

BLAST of Cla97C03G064220 vs. NCBI nr
Match: NP_001284441.1 (L-ascorbate oxidase [Cucumis melo] >AAF35911.2 ascorbate oxidase AO4 [Cucumis melo])

HSP 1 Score: 535.8 bits (1379), Expect = 2.9e-148
Identity = 255/306 (83.33%), Postives = 273/306 (89.22%), Query Frame = 0


Query: 2   PFLVIFLVLSIMSGFGITLSEA-VPKVRHYKWEVEYMFWYPDCVENIVMGINGQFPGPTI 61
           PFLV F VLSI+ GFGITLSEA  PK+RHYKWEVEYMFW PDCVENIVMGINGQFPGPTI
Sbjct: 13  PFLV-FFVLSIIFGFGITLSEAGFPKIRHYKWEVEYMFWSPDCVENIVMGINGQFPGPTI 72

Query: 62  RANVGDTIVVEMINKLSTEGLVIHWHGILQRGTPWADGTASISQCATNPGETFIYRFVVD 121
           RAN GD +VVE+ NKL TEG+VIHWHGILQRGTPWADGTASISQCA NPGETF YRFVVD
Sbjct: 73  RANAGDMVVVELTNKLHTEGVVIHWHGILQRGTPWADGTASISQCAINPGETFTYRFVVD 132

Query: 122 KAGTYFYHGHLGMQRAAGLYGSLIVDLEEGKKEPFHYDEDITLLLSDWWHKTVQKQEVGL 181
           KAGTYFYHGHLGMQR+AGLYGSLIVD +EG  EPFHYDE+I LLLSDWWH++V KQEVGL
Sbjct: 133 KAGTYFYHGHLGMQRSAGLYGSLIVDPQEGTSEPFHYDEEINLLLSDWWHQSVHKQEVGL 192

Query: 182 NSNPMHWINEPQAILINGRGQTNCSIAAKYNTSLKQCELKGDEQCAPFILHVKPNKTYRI 241
           +S PM WI EPQ+ILINGRGQ +CSIAAKY   LKQCEL G EQCAPFILHV P K YRI
Sbjct: 193 SSKPMRWIGEPQSILINGRGQFDCSIAAKYKNGLKQCELSGKEQCAPFILHVHPKKIYRI 252

Query: 242 RIASTTSLSALNFAIRNHQLLVVEADGNYVQPFLTSDIDIYSGESYSVLITTDQPASENY 301
           RIASTT+L++LNFAI NH+LLVVEADGNYVQPF TSDIDIYSGESYSVLITTDQ  SENY
Sbjct: 253 RIASTTALASLNFAIGNHKLLVVEADGNYVQPFSTSDIDIYSGESYSVLITTDQNPSENY 312

Query: 302 WVSVGV 307
           WVS+GV
Sbjct: 313 WVSIGV 317

[Close]

BLAST of Cla97C03G064220 vs. NCBI nr
Match: XP_004149015.1 (L-ascorbate oxidase [Cucumis sativus] >P14133.1 RecName: Full=L-ascorbate oxidase; Short=ASO; Short=Ascorbase; Flags: Precursor [Cucumis sativus] >AAA33119.1 ascorbate oxidase precursor (EC 1.10.3.3) [Cucumis sativus] >KGN66418.1 hypothetical protein Csa_007350 [Cucumis sativus])

HSP 1 Score: 533.5 bits (1373), Expect = 1.4e-147
Identity = 256/328 (78.05%), Postives = 282/328 (85.98%), Query Frame = 0


Query: 2   PFLVIFLVLSIMSGFGITLSEA-VPKVRHYKWEVEYMFWYPDCVENIVMGINGQFPGPTI 61
           PFL  FLVLSI+ GFGITLSEA  PK++HYKW+VEYMFW PDCVENIVMGING+FPGPTI
Sbjct: 13  PFL-SFLVLSIIFGFGITLSEAGFPKIKHYKWDVEYMFWSPDCVENIVMGINGEFPGPTI 72

Query: 62  RANVGDTIVVEMINKLSTEGLVIHWHGILQRGTPWADGTASISQCATNPGETFIYRFVVD 121
           RAN GD +VVE+ NKL TEG+VIHWHGILQRGTPWADGTASISQCA NPGETF YRFVVD
Sbjct: 73  RANAGDIVVVELTNKLHTEGVVIHWHGILQRGTPWADGTASISQCAINPGETFTYRFVVD 132

Query: 122 KAGTYFYHGHLGMQRAAGLYGSLIVDLEEGKKEPFHYDEDITLLLSDWWHKTVQKQEVGL 181
           KAGTYFYHGHLGMQR+AGLYGSLIVD  EG+ EPFHYDE+I LLLSDWWH++V KQEVGL
Sbjct: 133 KAGTYFYHGHLGMQRSAGLYGSLIVDPPEGRSEPFHYDEEINLLLSDWWHQSVHKQEVGL 192

Query: 182 NSNPMHWINEPQAILINGRGQTNCSIAAKYNTSLKQCELKGDEQCAPFILHVKPNKTYRI 241
           +S PM WI EPQ+ILING+GQ +CSIAAKYN  LKQCEL G E+CAPFILHV+P KTYRI
Sbjct: 193 SSKPMRWIGEPQSILINGKGQFDCSIAAKYNQGLKQCELSGKEKCAPFILHVQPKKTYRI 252

Query: 242 RIASTTSLSALNFAIRNHQLLVVEADGNYVQPFLTSDIDIYSGESYSVLITTDQPASENY 301
           RIASTT+L++LNFAI NH+LLVVEADGNYVQPF+TSDIDIYSGESYSVLITTDQ   ENY
Sbjct: 253 RIASTTALASLNFAIGNHELLVVEADGNYVQPFVTSDIDIYSGESYSVLITTDQNPLENY 312

Query: 302 WVSVGVPRAATQNASGTNYPQLPPQLRL 329
           WVS+GV             P+ PP L L
Sbjct: 313 WVSIGV---------RARLPKTPPGLTL 330

[Close]

BLAST of Cla97C03G064220 vs. ExPASy Swiss-Prot
Match: P14133 (L-ascorbate oxidase OS=Cucumis sativus OX=3659 PE=1 SV=1)

HSP 1 Score: 533.5 bits (1373), Expect = 1.9e-150
Identity = 256/328 (78.05%), Postives = 282/328 (85.98%), Query Frame = 0


Query: 2   PFLVIFLVLSIMSGFGITLSEA-VPKVRHYKWEVEYMFWYPDCVENIVMGINGQFPGPTI 61
           PFL  FLVLSI+ GFGITLSEA  PK++HYKW+VEYMFW PDCVENIVMGING+FPGPTI
Sbjct: 13  PFL-SFLVLSIIFGFGITLSEAGFPKIKHYKWDVEYMFWSPDCVENIVMGINGEFPGPTI 72

Query: 62  RANVGDTIVVEMINKLSTEGLVIHWHGILQRGTPWADGTASISQCATNPGETFIYRFVVD 121
           RAN GD +VVE+ NKL TEG+VIHWHGILQRGTPWADGTASISQCA NPGETF YRFVVD
Sbjct: 73  RANAGDIVVVELTNKLHTEGVVIHWHGILQRGTPWADGTASISQCAINPGETFTYRFVVD 132

Query: 122 KAGTYFYHGHLGMQRAAGLYGSLIVDLEEGKKEPFHYDEDITLLLSDWWHKTVQKQEVGL 181
           KAGTYFYHGHLGMQR+AGLYGSLIVD  EG+ EPFHYDE+I LLLSDWWH++V KQEVGL
Sbjct: 133 KAGTYFYHGHLGMQRSAGLYGSLIVDPPEGRSEPFHYDEEINLLLSDWWHQSVHKQEVGL 192

Query: 182 NSNPMHWINEPQAILINGRGQTNCSIAAKYNTSLKQCELKGDEQCAPFILHVKPNKTYRI 241
           +S PM WI EPQ+ILING+GQ +CSIAAKYN  LKQCEL G E+CAPFILHV+P KTYRI
Sbjct: 193 SSKPMRWIGEPQSILINGKGQFDCSIAAKYNQGLKQCELSGKEKCAPFILHVQPKKTYRI 252

Query: 242 RIASTTSLSALNFAIRNHQLLVVEADGNYVQPFLTSDIDIYSGESYSVLITTDQPASENY 301
           RIASTT+L++LNFAI NH+LLVVEADGNYVQPF+TSDIDIYSGESYSVLITTDQ   ENY
Sbjct: 253 RIASTTALASLNFAIGNHELLVVEADGNYVQPFVTSDIDIYSGESYSVLITTDQNPLENY 312

Query: 302 WVSVGVPRAATQNASGTNYPQLPPQLRL 329
           WVS+GV             P+ PP L L
Sbjct: 313 WVSIGV---------RARLPKTPPGLTL 330

[Close]

BLAST of Cla97C03G064220 vs. ExPASy Swiss-Prot
Match: P24792 (L-ascorbate oxidase OS=Cucurbita maxima OX=3661 GN=AAO PE=1 SV=2)

HSP 1 Score: 497.3 bits (1279), Expect = 1.5e-139
Identity = 237/326 (72.70%), Postives = 267/326 (81.90%), Query Frame = 0


Query: 3   FLVIFLVLSIMSGFGITLSEAVPKVRHYKWEVEYMFWYPDCVENIVMGINGQFPGPTIRA 62
           FL++F    ++  FGI  S    ++RHYKWEVEYMFW PDC ENIVMGINGQFPGPTIRA
Sbjct: 12  FLILFF-FGLILAFGI--SSEGSQIRHYKWEVEYMFWAPDCNENIVMGINGQFPGPTIRA 71

Query: 63  NVGDTIVVEMINKLSTEGLVIHWHGILQRGTPWADGTASISQCATNPGETFIYRFVVDKA 122
           N GDT+VVE+INKL TEG+VIHWHGILQRGTPWADGTASISQCA NPGETF Y F VD  
Sbjct: 72  NAGDTVVVELINKLHTEGVVIHWHGILQRGTPWADGTASISQCAINPGETFFYNFTVDNP 131

Query: 123 GTYFYHGHLGMQRAAGLYGSLIVDLEEGKKEPFHYDEDITLLLSDWWHKTVQKQEVGLNS 182
           GT+FYHGHLGMQR+AGLYGSLIVD  +GKKEPFHYD +I LLLSDWWH+++ KQEVGL+S
Sbjct: 132 GTFFYHGHLGMQRSAGLYGSLIVDPPQGKKEPFHYDGEINLLLSDWWHQSIHKQEVGLSS 191

Query: 183 NPMHWINEPQAILINGRGQTNCSIAAKYNTSLKQCELKGDEQCAPFILHVKPNKTYRIRI 242
            P+ WI EPQ IL+NGRGQ +CSIAAKY+++L+ C+LKG E CAP+I HV P KTYRIRI
Sbjct: 192 KPIRWIGEPQTILLNGRGQFDCSIAAKYDSNLEPCKLKGSEPCAPYIFHVMPKKTYRIRI 251

Query: 243 ASTTSLSALNFAIRNHQLLVVEADGNYVQPFLTSDIDIYSGESYSVLITTDQPASENYWV 302
           ASTT+L+ALNFAI NH LLVVEADGNYVQPF TSDIDIYSGESYSVLITTDQ  SENYWV
Sbjct: 252 ASTTALAALNFAIGNHPLLVVEADGNYVQPFYTSDIDIYSGESYSVLITTDQNPSENYWV 311

Query: 303 SVGVPRAATQNASGTNYPQLPPQLRL 329
           SVG         +   +P  PP L L
Sbjct: 312 SVG---------TRGRHPNTPPGLTL 325

[Close]

BLAST of Cla97C03G064220 vs. ExPASy Swiss-Prot
Match: P37064 (L-ascorbate oxidase OS=Cucurbita pepo var. melopepo OX=3665 PE=1 SV=1)

HSP 1 Score: 493.0 bits (1268), Expect = 2.8e-138
Identity = 228/303 (75.25%), Postives = 256/303 (84.49%), Query Frame = 0


Query: 26  KVRHYKWEVEYMFWYPDCVENIVMGINGQFPGPTIRANVGDTIVVEMINKLSTEGLVIHW 85
           ++RHYKWEVEYMFW P+C ENIVMGINGQFPGPTIRAN GD++VVE+ NKL TEG+VIHW
Sbjct: 2   QIRHYKWEVEYMFWAPNCNENIVMGINGQFPGPTIRANAGDSVVVELTNKLHTEGVVIHW 61

Query: 86  HGILQRGTPWADGTASISQCATNPGETFIYRFVVDKAGTYFYHGHLGMQRAAGLYGSLIV 145
           HGILQRGTPWADGTASISQCA NPGETF Y F VD  GT+FYHGHLGMQR+AGLYGSLIV
Sbjct: 62  HGILQRGTPWADGTASISQCAINPGETFFYNFTVDNPGTFFYHGHLGMQRSAGLYGSLIV 121

Query: 146 DLEEGKKEPFHYDEDITLLLSDWWHKTVQKQEVGLNSNPMHWINEPQAILINGRGQTNCS 205
           D  +GKKEPFHYD +I LLLSDWWH+++ KQEVGL+S P+ WI EPQ IL+NGRGQ +CS
Sbjct: 122 DPPQGKKEPFHYDGEINLLLSDWWHQSIHKQEVGLSSKPIRWIGEPQTILLNGRGQFDCS 181

Query: 206 IAAKYNTSLKQCELKGDEQCAPFILHVKPNKTYRIRIASTTSLSALNFAIRNHQLLVVEA 265
           IAAKY+++L+ C+LKG E CAP+I HV P KTYRIRIASTT+L+ALNFAI NHQLLVVEA
Sbjct: 182 IAAKYDSNLEPCKLKGSESCAPYIFHVSPKKTYRIRIASTTALAALNFAIGNHQLLVVEA 241

Query: 266 DGNYVQPFLTSDIDIYSGESYSVLITTDQPASENYWVSVGVPRAATQNASGTNYPQLPPQ 325
           DGNYVQPF TSDIDIYSGESYSVLITTDQ  SENYWVSVG         +   +P  PP 
Sbjct: 242 DGNYVQPFYTSDIDIYSGESYSVLITTDQNPSENYWVSVG---------TRARHPNTPPG 295

Query: 326 LRL 329
           L L
Sbjct: 302 LTL 295

[Close]

BLAST of Cla97C03G064220 vs. ExPASy Swiss-Prot
Match: Q40588 (L-ascorbate oxidase OS=Nicotiana tabacum OX=4097 GN=AAO PE=2 SV=1)

HSP 1 Score: 434.5 bits (1116), Expect = 1.2e-120
Identity = 206/336 (61.31%), Postives = 253/336 (75.30%), Query Frame = 0


Query: 3   FLVIFLVLSIMSGFGITLSEAVPKVRHYKWEVEYMFWYPDCVENIVMGINGQFPGPTIRA 62
           FL  FL+  I+     + S    K RH+KW+VEY+ W PD  E++VMGINGQFPGPTIRA
Sbjct: 6   FLFFFLLPLILLELSSSRSVMAAKTRHFKWDVEYIHWSPDGEESVVMGINGQFPGPTIRA 65

Query: 63  NVGDTIVVEMINKLSTEGLVIHWHGILQRGTPWADGTASISQCATNPGETFIYRFVVDKA 122
             GDT+ V + NKL TEG+VIHWHGI Q GTPWADGTA+ISQCA NPGETF+YRF VDKA
Sbjct: 66  KAGDTVAVHLTNKLHTEGVVIHWHGIRQIGTPWADGTAAISQCAINPGETFLYRFKVDKA 125

Query: 123 GTYFYHGHLGMQRAAGLYGSLIVDLEEGKKEPFHYDEDITLLLSDWWHKTVQKQEVGLNS 182
           GTYFYHGH GMQR+AGLYGSLIV++ EG+KEPFHYD +  LLLSDWWHK   +QEV L+S
Sbjct: 126 GTYFYHGHYGMQRSAGLYGSLIVEVGEGEKEPFHYDGEFNLLLSDWWHKGSHEQEVDLSS 185

Query: 183 NPMHWINEPQAILINGRGQTNCSIAAKYN-TSLKQCELKGDEQCAPFILHVKPNKTYRIR 242
           NP+ WI EPQ +L+NGRGQ NCS+AA+++   L QC+L+G EQ AP IL V+PNK YR+R
Sbjct: 186 NPLRWIGEPQTLLLNGRGQYNCSLAARFSKPPLPQCKLRGGEQYAPQILRVRPNKIYRLR 245

Query: 243 IASTTSLSALNFAIRNHQLLVVEADGNYVQPFLTSDIDIYSGESYSVLITTDQPASENYW 302
           +ASTT+L +L+ AI  H+++VVEADGNYVQPF   D+DIYSGESYSVL  TDQ  ++NYW
Sbjct: 246 VASTTALGSLSLAIGGHKMVVVEADGNYVQPFSVQDMDIYSGESYSVLFKTDQDPTKNYW 305

Query: 303 VSVGV-------PRAAT----QNASGTNYPQLPPQL 327
           +S+ V       P+  T       S + +P LPP +
Sbjct: 306 ISINVRGREPKTPQGLTLLNYLPNSASKFPTLPPPI 341

[Close]

BLAST of Cla97C03G064220 vs. ExPASy Swiss-Prot
Match: M4DUF2 (L-ascorbate oxidase OS=Brassica rapa subsp. pekinensis OX=51351 GN=AO PE=2 SV=1)

HSP 1 Score: 418.7 bits (1075), Expect = 6.7e-116
Identity = 194/313 (61.98%), Postives = 240/313 (76.68%), Query Frame = 0


Query: 27  VRHYKWEVEYMFWYPDCVENIVMGINGQFPGPTIRANVGDTIVVEMINKLSTEGLVIHWH 86
           VR Y WEVEY F +PDC E +VM +NGQFPGPTI A  GDTIVV + NKL+TEGLVIHWH
Sbjct: 20  VREYAWEVEYKFGWPDCKEGMVMAVNGQFPGPTIHALAGDTIVVHLTNKLATEGLVIHWH 79

Query: 87  GILQRGTPWADGTASISQCATNPGETFIYRFVVDKAGTYFYHGHLGMQRAAGLYGSLIVD 146
           GI Q G+PWADG A ++QCA +PGETF Y F VDK GT+FYHGH GMQR+AGLYGSLI+D
Sbjct: 80  GIRQLGSPWADGAAGVTQCAISPGETFTYNFTVDKPGTHFYHGHYGMQRSAGLYGSLIID 139

Query: 147 LEEGKKEPFHYDEDITLLLSDWWHKTVQKQEVGLNSNPMHWINEPQAILINGRGQTNCSI 206
           + +GKKEP  YD +  LLLSDWWH+ V  QE+GL+S PM WI E Q+ILINGRGQ NCS+
Sbjct: 140 VAKGKKEPLRYDGEFNLLLSDWWHEDVLSQEIGLSSRPMRWIGEAQSILINGRGQFNCSL 199

Query: 207 AAKY-NTSLKQCELKGDEQCAPFILHVKPNKTYRIRIASTTSLSALNFAIRNHQLLVVEA 266
           AA++ +TSL  C  K  +QCAP  LHV+PNKTYRIR+AS+T+L++LNFA++ H+L+VVEA
Sbjct: 200 AAQFSSTSLPTCTFKEGDQCAPQRLHVEPNKTYRIRLASSTALASLNFAVQGHKLVVVEA 259

Query: 267 DGNYVQPFLTSDIDIYSGESYSVLITTDQPASENYWVSVGVPRAATQNASGTNYPQLPPQ 326
           DGNY+ PF T DIDIYSGE+YSVL+TTDQ  S+NY+++ GV             P  PP 
Sbjct: 260 DGNYITPFTTDDIDIYSGETYSVLLTTDQDPSQNYYITAGV---------RGRKPNTPPA 319

Query: 327 LRLQITHFSAPGN 339
           L + + + +AP +
Sbjct: 320 LTV-LNYVTAPSS 322

[Close]

BLAST of Cla97C03G064220 vs. ExPASy TrEMBL
Match: A0A5A7UMY3 (L-ascorbate oxidase OS=Cucumis melo var. makuwa OX=1194695 GN=E6C27_scaffold181G00010 PE=3 SV=1)

HSP 1 Score: 538.1 bits (1385), Expect = 2.8e-149
Identity = 256/306 (83.66%), Postives = 274/306 (89.54%), Query Frame = 0


Query: 2   PFLVIFLVLSIMSGFGITLSEA-VPKVRHYKWEVEYMFWYPDCVENIVMGINGQFPGPTI 61
           PFLV F VLSI+ GFGITLSEA  PK+RHYKWEVEYMFW PDCVENIVMGINGQFPGPTI
Sbjct: 13  PFLV-FFVLSIIFGFGITLSEAGFPKIRHYKWEVEYMFWSPDCVENIVMGINGQFPGPTI 72

Query: 62  RANVGDTIVVEMINKLSTEGLVIHWHGILQRGTPWADGTASISQCATNPGETFIYRFVVD 121
           RAN GD +VVE+ NKL TEG+VIHWHGILQRGTPWADGTASISQCA NPGETF YRFVVD
Sbjct: 73  RANAGDMVVVELTNKLHTEGVVIHWHGILQRGTPWADGTASISQCAINPGETFTYRFVVD 132

Query: 122 KAGTYFYHGHLGMQRAAGLYGSLIVDLEEGKKEPFHYDEDITLLLSDWWHKTVQKQEVGL 181
           KAGTYFYHGHLGMQR+AGLYGSLIVD +EG  EPFHYDE+I LLLSDWWH++V KQEVGL
Sbjct: 133 KAGTYFYHGHLGMQRSAGLYGSLIVDPQEGTSEPFHYDEEINLLLSDWWHQSVHKQEVGL 192

Query: 182 NSNPMHWINEPQAILINGRGQTNCSIAAKYNTSLKQCELKGDEQCAPFILHVKPNKTYRI 241
           +S PM WI EPQ+ILINGRGQ +CSIAAKY   LKQCEL G EQCAPFILHV P KTYRI
Sbjct: 193 SSKPMRWIGEPQSILINGRGQFDCSIAAKYKNGLKQCELSGKEQCAPFILHVHPKKTYRI 252

Query: 242 RIASTTSLSALNFAIRNHQLLVVEADGNYVQPFLTSDIDIYSGESYSVLITTDQPASENY 301
           RIASTT+L++LNFAI NH+LLVVEADGNYVQPF TSDIDIYSGESYSVLITTDQ  SENY
Sbjct: 253 RIASTTALASLNFAIGNHKLLVVEADGNYVQPFSTSDIDIYSGESYSVLITTDQNPSENY 312

Query: 302 WVSVGV 307
           WVS+GV
Sbjct: 313 WVSIGV 317

[Close]

BLAST of Cla97C03G064220 vs. ExPASy TrEMBL
Match: Q9M5B4 (L-ascorbate oxidase OS=Cucumis melo OX=3656 GN=LOC103492479 PE=2 SV=1)

HSP 1 Score: 535.8 bits (1379), Expect = 1.4e-148
Identity = 255/306 (83.33%), Postives = 273/306 (89.22%), Query Frame = 0


Query: 2   PFLVIFLVLSIMSGFGITLSEA-VPKVRHYKWEVEYMFWYPDCVENIVMGINGQFPGPTI 61
           PFLV F VLSI+ GFGITLSEA  PK+RHYKWEVEYMFW PDCVENIVMGINGQFPGPTI
Sbjct: 13  PFLV-FFVLSIIFGFGITLSEAGFPKIRHYKWEVEYMFWSPDCVENIVMGINGQFPGPTI 72

Query: 62  RANVGDTIVVEMINKLSTEGLVIHWHGILQRGTPWADGTASISQCATNPGETFIYRFVVD 121
           RAN GD +VVE+ NKL TEG+VIHWHGILQRGTPWADGTASISQCA NPGETF YRFVVD
Sbjct: 73  RANAGDMVVVELTNKLHTEGVVIHWHGILQRGTPWADGTASISQCAINPGETFTYRFVVD 132

Query: 122 KAGTYFYHGHLGMQRAAGLYGSLIVDLEEGKKEPFHYDEDITLLLSDWWHKTVQKQEVGL 181
           KAGTYFYHGHLGMQR+AGLYGSLIVD +EG  EPFHYDE+I LLLSDWWH++V KQEVGL
Sbjct: 133 KAGTYFYHGHLGMQRSAGLYGSLIVDPQEGTSEPFHYDEEINLLLSDWWHQSVHKQEVGL 192

Query: 182 NSNPMHWINEPQAILINGRGQTNCSIAAKYNTSLKQCELKGDEQCAPFILHVKPNKTYRI 241
           +S PM WI EPQ+ILINGRGQ +CSIAAKY   LKQCEL G EQCAPFILHV P K YRI
Sbjct: 193 SSKPMRWIGEPQSILINGRGQFDCSIAAKYKNGLKQCELSGKEQCAPFILHVHPKKIYRI 252

Query: 242 RIASTTSLSALNFAIRNHQLLVVEADGNYVQPFLTSDIDIYSGESYSVLITTDQPASENY 301
           RIASTT+L++LNFAI NH+LLVVEADGNYVQPF TSDIDIYSGESYSVLITTDQ  SENY
Sbjct: 253 RIASTTALASLNFAIGNHKLLVVEADGNYVQPFSTSDIDIYSGESYSVLITTDQNPSENY 312

Query: 302 WVSVGV 307
           WVS+GV
Sbjct: 313 WVSIGV 317

[Close]

BLAST of Cla97C03G064220 vs. ExPASy TrEMBL
Match: E7BBM8 (L-ascorbate oxidase OS=Cucumis sativus OX=3659 GN=aso PE=2 SV=1)

HSP 1 Score: 533.5 bits (1373), Expect = 6.9e-148
Identity = 256/328 (78.05%), Postives = 282/328 (85.98%), Query Frame = 0


Query: 2   PFLVIFLVLSIMSGFGITLSEA-VPKVRHYKWEVEYMFWYPDCVENIVMGINGQFPGPTI 61
           PFL  FLVLSI+ GFGITLSEA  PK++HYKW+VEYMFW PDCVENIVMGING+FPGPTI
Sbjct: 13  PFL-SFLVLSIIFGFGITLSEAGFPKIKHYKWDVEYMFWSPDCVENIVMGINGEFPGPTI 72

Query: 62  RANVGDTIVVEMINKLSTEGLVIHWHGILQRGTPWADGTASISQCATNPGETFIYRFVVD 121
           RAN GD +VVE+ NKL TEG+VIHWHGILQRGTPWADGTASISQCA NPGETF YRFVVD
Sbjct: 73  RANAGDIVVVELTNKLHTEGVVIHWHGILQRGTPWADGTASISQCAINPGETFTYRFVVD 132

Query: 122 KAGTYFYHGHLGMQRAAGLYGSLIVDLEEGKKEPFHYDEDITLLLSDWWHKTVQKQEVGL 181
           KAGTYFYHGHLGMQR+AGLYGSLIVD  EG+ EPFHYDE+I LLLSDWWH++V KQEVGL
Sbjct: 133 KAGTYFYHGHLGMQRSAGLYGSLIVDPPEGRSEPFHYDEEINLLLSDWWHQSVHKQEVGL 192

Query: 182 NSNPMHWINEPQAILINGRGQTNCSIAAKYNTSLKQCELKGDEQCAPFILHVKPNKTYRI 241
           +S PM WI EPQ+ILING+GQ +CSIAAKYN  LKQCEL G E+CAPFILHV+P KTYRI
Sbjct: 193 SSKPMRWIGEPQSILINGKGQFDCSIAAKYNQGLKQCELSGKEKCAPFILHVQPKKTYRI 252

Query: 242 RIASTTSLSALNFAIRNHQLLVVEADGNYVQPFLTSDIDIYSGESYSVLITTDQPASENY 301
           RIASTT+L++LNFAI NH+LLVVEADGNYVQPF+TSDIDIYSGESYSVLITTDQ   ENY
Sbjct: 253 RIASTTALASLNFAIGNHELLVVEADGNYVQPFVTSDIDIYSGESYSVLITTDQNPLENY 312

Query: 302 WVSVGVPRAATQNASGTNYPQLPPQLRL 329
           WVS+GV             P+ PP L L
Sbjct: 313 WVSIGV---------RARLPKTPPGLTL 330

[Close]

BLAST of Cla97C03G064220 vs. ExPASy TrEMBL
Match: A0A0A0LXB9 (L-ascorbate oxidase OS=Cucumis sativus OX=3659 GN=Csa_1G604040 PE=3 SV=1)

HSP 1 Score: 533.5 bits (1373), Expect = 6.9e-148
Identity = 256/328 (78.05%), Postives = 282/328 (85.98%), Query Frame = 0


Query: 2   PFLVIFLVLSIMSGFGITLSEA-VPKVRHYKWEVEYMFWYPDCVENIVMGINGQFPGPTI 61
           PFL  FLVLSI+ GFGITLSEA  PK++HYKW+VEYMFW PDCVENIVMGING+FPGPTI
Sbjct: 13  PFL-SFLVLSIIFGFGITLSEAGFPKIKHYKWDVEYMFWSPDCVENIVMGINGEFPGPTI 72

Query: 62  RANVGDTIVVEMINKLSTEGLVIHWHGILQRGTPWADGTASISQCATNPGETFIYRFVVD 121
           RAN GD +VVE+ NKL TEG+VIHWHGILQRGTPWADGTASISQCA NPGETF YRFVVD
Sbjct: 73  RANAGDIVVVELTNKLHTEGVVIHWHGILQRGTPWADGTASISQCAINPGETFTYRFVVD 132

Query: 122 KAGTYFYHGHLGMQRAAGLYGSLIVDLEEGKKEPFHYDEDITLLLSDWWHKTVQKQEVGL 181
           KAGTYFYHGHLGMQR+AGLYGSLIVD  EG+ EPFHYDE+I LLLSDWWH++V KQEVGL
Sbjct: 133 KAGTYFYHGHLGMQRSAGLYGSLIVDPPEGRSEPFHYDEEINLLLSDWWHQSVHKQEVGL 192

Query: 182 NSNPMHWINEPQAILINGRGQTNCSIAAKYNTSLKQCELKGDEQCAPFILHVKPNKTYRI 241
           +S PM WI EPQ+ILING+GQ +CSIAAKYN  LKQCEL G E+CAPFILHV+P KTYRI
Sbjct: 193 SSKPMRWIGEPQSILINGKGQFDCSIAAKYNQGLKQCELSGKEKCAPFILHVQPKKTYRI 252

Query: 242 RIASTTSLSALNFAIRNHQLLVVEADGNYVQPFLTSDIDIYSGESYSVLITTDQPASENY 301
           RIASTT+L++LNFAI NH+LLVVEADGNYVQPF+TSDIDIYSGESYSVLITTDQ   ENY
Sbjct: 253 RIASTTALASLNFAIGNHELLVVEADGNYVQPFVTSDIDIYSGESYSVLITTDQNPLENY 312

Query: 302 WVSVGVPRAATQNASGTNYPQLPPQLRL 329
           WVS+GV             P+ PP L L
Sbjct: 313 WVSIGV---------RARLPKTPPGLTL 330

[Close]

BLAST of Cla97C03G064220 vs. ExPASy TrEMBL
Match: A0A0A0LZW2 (Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_1G602540 PE=3 SV=1)

HSP 1 Score: 531.6 bits (1368), Expect = 2.6e-147
Identity = 249/308 (80.84%), Postives = 276/308 (89.61%), Query Frame = 0


Query: 1   MPFLVIFLVLSIMSGFGITLSEAVPKVRHYKWEVEYMFWYPDCVENIVMGINGQFPGPTI 60
           MPF  +F +LSI+ GFGITLS+AV + RHY WEVEYMFW PDC EN+VMGINGQFPGPTI
Sbjct: 13  MPFPAMFFLLSIIFGFGITLSDAVSQTRHYTWEVEYMFWSPDCKENLVMGINGQFPGPTI 72

Query: 61  RANVGDTIVVEMINKLSTEGLVIHWHGILQRGTPWADGTASISQCATNPGETFIYRFVVD 120
           RANVGDT+VVEMINKLSTE +VIHWHGILQ+GTPWADGTASISQCATNPGE+F Y+FVVD
Sbjct: 73  RANVGDTVVVEMINKLSTEDVVIHWHGILQKGTPWADGTASISQCATNPGESFTYQFVVD 132

Query: 121 KAGTYFYHGHLGMQRAAGLYGSLIVDLEEGKKEPFHYDEDITLLLSDWWHKTVQKQEVGL 180
           K GTYFYHGHLGMQRAAGLYGSLIVD EEG+KEPFHYDE+I LLLSDWWH+++ KQE+GL
Sbjct: 133 KPGTYFYHGHLGMQRAAGLYGSLIVDPEEGEKEPFHYDEEINLLLSDWWHESILKQEIGL 192

Query: 181 NSNPMHWINEPQAILINGRGQTNCSIAAKYNT--SLKQCELKGDEQCAPFILHVKPNKTY 240
           +S+PM WI EPQ+ILINGRGQ+NCSIAAKY     ++QC+L G EQCAPFIL VKPNK Y
Sbjct: 193 SSSPMRWIGEPQSILINGRGQSNCSIAAKYMNMKKMEQCKLSGSEQCAPFILEVKPNKIY 252

Query: 241 RIRIASTTSLSALNFAIRNHQLLVVEADGNYVQPFLTSDIDIYSGESYSVLITTDQPASE 300
           RIRI STTSLSALNFAI  H+LLVVEADGNYVQPFLTSDIDIYSGESYSVLI TDQ  SE
Sbjct: 253 RIRITSTTSLSALNFAIGKHKLLVVEADGNYVQPFLTSDIDIYSGESYSVLINTDQKPSE 312

Query: 301 NYWVSVGV 307
           NYWVS+G+
Sbjct: 313 NYWVSLGI 320

[Close]

BLAST of Cla97C03G064220 vs. TAIR 10
Match: AT5G21105.1 (Plant L-ascorbate oxidase )

HSP 1 Score: 419.9 bits (1078), Expect = 2.1e-117
Identity = 196/308 (63.64%), Postives = 237/308 (76.95%), Query Frame = 0


Query: 7   FLVLSIMSGFGITLSEAV------PKVRHYKWEVEYMFWYPDCVENIVMGINGQFPGPTI 66
           F  +S M  + I L  AV        VR Y WEVEY +W PDC E  VM +NG+FPGPTI
Sbjct: 11  FTYISQMGVWWIVLVVAVLTHTASAAVREYHWEVEYKYWSPDCKEGAVMTVNGEFPGPTI 70

Query: 67  RANVGDTIVVEMINKLSTEGLVIHWHGILQRGTPWADGTASISQCATNPGETFIYRFVVD 126
           +A  GDTIVV + NKL+TEGLVIHWHGI Q G+PWADG A ++QCA NPGETF Y F V+
Sbjct: 71  KAFAGDTIVVNLTNKLTTEGLVIHWHGIRQFGSPWADGAAGVTQCAINPGETFTYNFTVE 130

Query: 127 KAGTYFYHGHLGMQRAAGLYGSLIVDLEEGKKEPFHYDEDITLLLSDWWHKTVQKQEVGL 186
           K GT+FYHGH GMQR+AGLYGSLIVD+ +GK E   YD +  LLLSDWWH+ +  QE+GL
Sbjct: 131 KPGTHFYHGHYGMQRSAGLYGSLIVDVAKGKSERLRYDGEFNLLLSDWWHEAIPSQELGL 190

Query: 187 NSNPMHWINEPQAILINGRGQTNCSIAAKY--NTSLKQCELKGDEQCAPFILHVKPNKTY 246
           +S PM WI E Q+ILINGRGQ NCS+AA++  NTSL  C  K  +QCAP ILHV+PNKTY
Sbjct: 191 SSKPMRWIGEAQSILINGRGQFNCSLAAQFSNNTSLPMCTFKEGDQCAPQILHVEPNKTY 250

Query: 247 RIRIASTTSLSALNFAIRNHQLLVVEADGNYVQPFLTSDIDIYSGESYSVLITTDQPASE 306
           RIR++STT+L++LN A++ H+L+VVEADGNY+ PF T DIDIYSGESYSVL+TTDQ  S+
Sbjct: 251 RIRLSSTTALASLNLAVQGHKLVVVEADGNYITPFTTDDIDIYSGESYSVLLTTDQDPSQ 310

[Close]

BLAST of Cla97C03G064220 vs. TAIR 10
Match: AT5G21105.3 (Plant L-ascorbate oxidase )

HSP 1 Score: 419.9 bits (1078), Expect = 2.1e-117
Identity = 196/308 (63.64%), Postives = 237/308 (76.95%), Query Frame = 0


Query: 7   FLVLSIMSGFGITLSEAV------PKVRHYKWEVEYMFWYPDCVENIVMGINGQFPGPTI 66
           F  +S M  + I L  AV        VR Y WEVEY +W PDC E  VM +NG+FPGPTI
Sbjct: 11  FTYISQMGVWWIVLVVAVLTHTASAAVREYHWEVEYKYWSPDCKEGAVMTVNGEFPGPTI 70

Query: 67  RANVGDTIVVEMINKLSTEGLVIHWHGILQRGTPWADGTASISQCATNPGETFIYRFVVD 126
           +A  GDTIVV + NKL+TEGLVIHWHGI Q G+PWADG A ++QCA NPGETF Y F V+
Sbjct: 71  KAFAGDTIVVNLTNKLTTEGLVIHWHGIRQFGSPWADGAAGVTQCAINPGETFTYNFTVE 130

Query: 127 KAGTYFYHGHLGMQRAAGLYGSLIVDLEEGKKEPFHYDEDITLLLSDWWHKTVQKQEVGL 186
           K GT+FYHGH GMQR+AGLYGSLIVD+ +GK E   YD +  LLLSDWWH+ +  QE+GL
Sbjct: 131 KPGTHFYHGHYGMQRSAGLYGSLIVDVAKGKSERLRYDGEFNLLLSDWWHEAIPSQELGL 190

Query: 187 NSNPMHWINEPQAILINGRGQTNCSIAAKY--NTSLKQCELKGDEQCAPFILHVKPNKTY 246
           +S PM WI E Q+ILINGRGQ NCS+AA++  NTSL  C  K  +QCAP ILHV+PNKTY
Sbjct: 191 SSKPMRWIGEAQSILINGRGQFNCSLAAQFSNNTSLPMCTFKEGDQCAPQILHVEPNKTY 250

Query: 247 RIRIASTTSLSALNFAIRNHQLLVVEADGNYVQPFLTSDIDIYSGESYSVLITTDQPASE 306
           RIR++STT+L++LN A++ H+L+VVEADGNY+ PF T DIDIYSGESYSVL+TTDQ  S+
Sbjct: 251 RIRLSSTTALASLNLAVQGHKLVVVEADGNYITPFTTDDIDIYSGESYSVLLTTDQDPSQ 310

[Close]

BLAST of Cla97C03G064220 vs. TAIR 10
Match: AT5G21100.1 (Plant L-ascorbate oxidase )

HSP 1 Score: 387.5 bits (994), Expect = 1.2e-107
Identity = 173/277 (62.45%), Postives = 218/277 (78.70%), Query Frame = 0


Query: 32  WEVEYMFWYPDCVENIVMGINGQFPGPTIRANVGDTIVVEMINKLSTEGLVIHWHGILQR 91
           WEVEY +W+PDC E IVM INGQFPGPTI A  GDT+++ ++NKLSTEG+VIHWHGI Q+
Sbjct: 28  WEVEYKYWWPDCKEGIVMAINGQFPGPTIDAVAGDTVIIHVVNKLSTEGVVIHWHGIRQK 87

Query: 92  GTPWADGTASISQCATNPGETFIYRFVVDKAGTYFYHGHLGMQRAAGLYGSLIVDLEEGK 151
           GTPWADG A ++QC  NPGETF Y+F+VDKAGT+FYHGH GMQR++GLYG LIV      
Sbjct: 88  GTPWADGAAGVTQCPINPGETFTYKFIVDKAGTHFYHGHYGMQRSSGLYGMLIV---RSP 147

Query: 152 KEPFHYDEDITLLLSDWWHKTVQKQEVGLNSNPMHWINEPQAILINGRGQTNCSIAAKYN 211
           KE   YD +  LLLSDWWH+++  QE+ L+S PM WI EPQ++LINGRGQ NCS AA +N
Sbjct: 148 KERLIYDGEFNLLLSDWWHQSIHAQELALSSRPMRWIGEPQSLLINGRGQFNCSQAAYFN 207

Query: 212 TSLKQ--CELKGDEQCAPFILHVKPNKTYRIRIASTTSLSALNFAIRNHQLLVVEADGNY 271
              ++  C  K ++QCAP  L V+PN+ YR+RIASTT+L++LN A++ HQL+VVEADGNY
Sbjct: 208 KGGEKDVCTFKENDQCAPQTLRVEPNRVYRLRIASTTALASLNLAVQGHQLVVVEADGNY 267

Query: 272 VQPFLTSDIDIYSGESYSVLITTDQPASENYWVSVGV 307
           V PF  +DID+YSGE+YSVL+ T+   S+ YW+SVGV
Sbjct: 268 VAPFTVNDIDVYSGETYSVLLKTNALPSKKYWISVGV 301

[Close]

BLAST of Cla97C03G064220 vs. TAIR 10
Match: AT4G39830.1 (Cupredoxin superfamily protein )

HSP 1 Score: 315.5 bits (807), Expect = 5.6e-86
Identity = 150/289 (51.90%), Postives = 193/289 (66.78%), Query Frame = 0


Query: 26  KVRHYKWEVEYMFWYPDCVENIVMGINGQFPGPTIRANVGDTIVVEMINKLSTEGLVIHW 85
           K+R +KWEV+Y F  PDC E +V+ ING+FPGPTI+A  GDTIVVE+ N   TE + +HW
Sbjct: 34  KIRRFKWEVKYEFKSPDCFEKLVITINGKFPGPTIKAQQGDTIVVELKNSFMTENVAVHW 93

Query: 86  HGILQRGTPWADGTASISQCATNPGETFIYRFVVDKAGTYFYHGHLGMQRAAGLYGSLIV 145
           HGI Q GTPW DG   ++QC   PGE FIY+FVVD+ GTY YH H GMQR +GL G + V
Sbjct: 94  HGIRQIGTPWFDGVEGVTQCPILPGEVFIYQFVVDRPGTYMYHSHYGMQRESGLIGMIQV 153

Query: 146 DLEEGKKEPFHYDEDITLLLSDWWHKTVQKQEVGLNSNPMHWINEPQAILINGRGQTNCS 205
                + EPF YD D   LL+DW+HK++ ++  GL S P  W+ EPQ+++I GRG+ NCS
Sbjct: 154 SPPATEPEPFTYDYDRNFLLTDWYHKSMSEKATGLASIPFKWVGEPQSLMIQGRGRFNCS 213

Query: 206 IAAKYNTSLKQ--CELKGDEQCAPFILHVKPNKTYRIRIASTTSLSALNFAIRNHQLLVV 265
                  SL    C +  +  C+ FIL V P KTYR+RI S T+LSAL+F I  H L VV
Sbjct: 214 NNLTTPPSLVSGVCNV-SNADCSRFILTVIPGKTYRLRIGSLTALSALSFQIEGHNLTVV 273

Query: 266 EADGNYVQPFLTSDIDIYSGESYSVLITTDQPASENYWVSVG-VPRAAT 312
           EADG+YV+PF   ++ +YSGE+YSVL+  DQ    NYW++   V R AT
Sbjct: 274 EADGHYVEPFTVKNLFVYSGETYSVLLKADQNPRRNYWITSSIVSRPAT 321

[Close]

BLAST of Cla97C03G064220 vs. TAIR 10
Match: AT5G21105.2 (Plant L-ascorbate oxidase )

HSP 1 Score: 167.9 bits (424), Expect = 1.5e-41
Identity = 79/117 (67.52%), Postives = 100/117 (85.47%), Query Frame = 0


Query: 192 QAILINGRGQTNCSIAAKY--NTSLKQCELKGDEQCAPFILHVKPNKTYRIRIASTTSLS 251
           ++ILINGRGQ NCS+AA++  NTSL  C  K  +QCAP ILHV+PNKTYRIR++STT+L+
Sbjct: 11  KSILINGRGQFNCSLAAQFSNNTSLPMCTFKEGDQCAPQILHVEPNKTYRIRLSSTTALA 70

Query: 252 ALNFAIRNHQLLVVEADGNYVQPFLTSDIDIYSGESYSVLITTDQPASENYWVSVGV 307
           +LN A++ H+L+VVEADGNY+ PF T DIDIYSGESYSVL+TTDQ  S+NY++SVGV
Sbjct: 71  SLNLAVQGHKLVVVEADGNYITPFTTDDIDIYSGESYSVLLTTDQDPSQNYYISVGV 127

The following BLAST results are available for this feature:

BLAST of Cla97C03G064220 vs. NCBI nr


Match Name	E-value	Identity	Description
XP_038878978.1	2.3e-153	80.49	L-ascorbate oxidase [Benincasa hispida]	[more]
XP_038880052.1	8.9e-150	78.05	L-ascorbate oxidase-like [Benincasa hispida]	[more]
KAA0055636.1	5.7e-149	83.66	L-ascorbate oxidase [Cucumis melo var. makuwa]	[more]
NP_001284441.1	2.9e-148	83.33	L-ascorbate oxidase [Cucumis melo] >AAF35911.2 ascorbate oxidase AO4 [Cucumis me...	[more]
XP_004149015.1	1.4e-147	78.05	L-ascorbate oxidase [Cucumis sativus] >P14133.1 RecName: Full=L-ascorbate oxidas...	[more]

BLAST of Cla97C03G064220 vs. ExPASy Swiss-Prot


Match Name	E-value	Identity	Description
P14133	1.9e-150	78.05	L-ascorbate oxidase OS=Cucumis sativus OX=3659 PE=1 SV=1	[more]
P24792	1.5e-139	72.70	L-ascorbate oxidase OS=Cucurbita maxima OX=3661 GN=AAO PE=1 SV=2	[more]
P37064	2.8e-138	75.25	L-ascorbate oxidase OS=Cucurbita pepo var. melopepo OX=3665 PE=1 SV=1	[more]
Q40588	1.2e-120	61.31	L-ascorbate oxidase OS=Nicotiana tabacum OX=4097 GN=AAO PE=2 SV=1	[more]
M4DUF2	6.7e-116	61.98	L-ascorbate oxidase OS=Brassica rapa subsp. pekinensis OX=51351 GN=AO PE=2 SV=1	[more]

BLAST of Cla97C03G064220 vs. ExPASy TrEMBL


Match Name	E-value	Identity	Description
A0A5A7UMY3	2.8e-149	83.66	L-ascorbate oxidase OS=Cucumis melo var. makuwa OX=1194695 GN=E6C27_scaffold181G...	[more]
Q9M5B4	1.4e-148	83.33	L-ascorbate oxidase OS=Cucumis melo OX=3656 GN=LOC103492479 PE=2 SV=1	[more]
E7BBM8	6.9e-148	78.05	L-ascorbate oxidase OS=Cucumis sativus OX=3659 GN=aso PE=2 SV=1	[more]
A0A0A0LXB9	6.9e-148	78.05	L-ascorbate oxidase OS=Cucumis sativus OX=3659 GN=Csa_1G604040 PE=3 SV=1	[more]
A0A0A0LZW2	2.6e-147	80.84	Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_1G602540 PE=3 SV=1	[more]

BLAST of Cla97C03G064220 vs. TAIR 10


Match Name	E-value	Identity	Description
AT5G21105.1	2.1e-117	63.64	Plant L-ascorbate oxidase	[more]
AT5G21105.3	2.1e-117	63.64	Plant L-ascorbate oxidase	[more]
AT5G21100.1	1.2e-107	62.45	Plant L-ascorbate oxidase	[more]
AT4G39830.1	5.6e-86	51.90	Cupredoxin superfamily protein	[more]
AT5G21105.2	1.5e-41	67.52	Plant L-ascorbate oxidase	[more]

InterPro

Analysis Name: InterPro Annotations of Watermelon (97103) v2.5
Date Performed: 2022-01-31


IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
IPR008972	Cupredoxin	GENE3D	2.60.40.420		coord: 155..348 e-value: 7.6E-56 score: 191.4
IPR008972	Cupredoxin	GENE3D	2.60.40.420		coord: 17..146 e-value: 9.5E-47 score: 160.7
IPR008972	Cupredoxin	SUPERFAMILY	49503	Cupredoxins	coord: 23..172
IPR008972	Cupredoxin	SUPERFAMILY	49503	Cupredoxins	coord: 154..316
IPR001117	Multicopper oxidase, type 1	PFAM	PF00394	Cu-oxidase	coord: 159..311 e-value: 4.4E-46 score: 156.9
IPR011707	Multicopper oxidase, N-termianl	PFAM	PF07732	Cu-oxidase_3	coord: 34..147 e-value: 1.1E-39 score: 135.1
None	No IPR available	PANTHER	PTHR11709:SF394	PLANT L-ASCORBATE OXIDASE	coord: 21..313
IPR045087	Multicopper oxidase	PANTHER	PTHR11709	MULTI-COPPER OXIDASE	coord: 21..313
IPR034259	Ascorbate oxidase, first cupredoxin domain	CDD	cd13845	CuRO_1_AAO	coord: 28..147 e-value: 1.00782E-84 score: 250.441

Relationships

The following mRNA feature(s) are a part of this gene:


Feature Name	Unique Name	Type
Cla97C03G064220.1	Cla97C03G064220.1	mRNA

GO Annotation

GO Assignments

This gene is annotated with the following GO terms.

Category	Term Accession	Term Name
cellular_component	GO:0005576	extracellular region
molecular_function	GO:0005507	copper ion binding
molecular_function	GO:0008447	L-ascorbate oxidase activity