Cla97C03G062600 (gene) Watermelon (97103) v2.5

Overview
NameCla97C03G062600
Typegene
OrganismCitrullus lanatus subsp. vulgaris cv. 97103 (Watermelon (97103) v2.5)
DescriptionRING-type E3 ubiquitin transferase
LocationCla97Chr03: 23846945 .. 23850304 (-)
RNA-Seq ExpressionCla97C03G062600
SyntenyCla97C03G062600
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: polypeptideexonCDS
Hold the cursor over a type above to highlight its positions in the sequence below.
ATGACGGAAATCGAGGTTCCTTCAGACTTTTTGTGCCCTATTTCTCTACAGATCATGAGAGATCCGGTGACGATCTCGACGGGGATCACATACGACAGAGAGAGCATCGAGACATGGCTCTTGTGTTGCAATAACCGACCCGTTGTCGTCCTGAGCTGCCCAGTGACAAAGCAGGCGTTGAGCGGTATGGATTTGACTCCGAACCATACGCTACGGCGGGTGATTCAAGGATGGTGCAATCTCAATAGGAGGCATGGGGTGGAGCAGATCCCAACCCCTAAGGGTGAGGGTGATAGAGGAGATGTTGTTAAGTTGATTCTTAAGGAGGCAGTGAGAGGTCCAAGAAGCTCAAGGCTTGAGTGCTTAAAGAGATTGAAATCTTTTGTGGATGAGAGTGAGAGGAACAAAGTCTACTTGCAGGCAAGCATTAATTATCAACATTTATCACAACTCTCTTCTTCTTCTTCTTCTTCTTCTTCTTCTTTTTTTTTTTTTTTTGAAGGAATTTTTTATCTGAAAGCTATTTGACTTTCTTTTTATACATTTTTTAAGATCTTCATAATCTTTTCTAAGATTTTCGTAACCATTTTTGGCCTGCTCAAGAATGTGTTAGAAAGAAGCACTTTTCACAACTTTGAAACATATTTTTTCTATAAGTTGTTCTGAAAATGGCAGTAATTAATTAAGTGCTTGTTTGAAGTTTTTTCATTATTTTAGATTGGATTATTAGACAGAAGAAAATTTTTTAAAAGGGTTATAAAAGGAAATTCAATTTCCCCTTTCGAAAATATGTCCCAACCTCTTCTTGAGGCATATATAATTCTTCTAGGATTATTTACACCCGGTTTAGTAACCATTTTATTTTTTACTTTTGGTTTTTGAAAATTAAACTTATAATCACCACTTTCGTATATTGATTGGTGCATCTTGTTGTTTATATTTTAAGGGTGTTTTAAAAAAACAAGCTAAGTTTTAAAAAGTAAAAGGAGTAAGCCCTATTTGGTAACAATTTGATTTTTTGGTTCTTGTTTTTTTTAAAATTAAGCCTATTTACATTCCAACTCCAAATTTATTTCTTTTTTTATCTACTTTTCACCAATAATTTAAAAAAATCAAGCTAAATTTTGAAAAGTAAAAAAAGTAGCTTTTAAAATTTTGCTTTTGTTTTTGTTTTTGGAATTTGGCTAAAAATTCAATCATTGTATTTAAGAAAAATCCGATTTTTTTTTTGTAAGAAATGGAGAGAAAATAGGCTTACTTTTCAAAAACCAAAAACAAAAAATCAAATGGTTACCAAATGGGGGCATAGTATTTCAAAACTTGTTTTTGTATTTGAAATTTGGCCAAAGATTCATATGCCTACATAGAAAATATGAAAACCATGGTAAAAAAGTTGTGTGAACCAAACTCAATTTACAAAAACCAACAATCAAAAATTGAATGGTTACCCCAATGGCACCTTAGTTTTTTAATTTTGATCAAGGTAGAGTATTGATTCCTATTATATGCAATGATTAAAATGTTGATGTTGATCATGGAAATGTCTGTGTCTCAATTTTACAAAAATATTAATGTTGATGGAAATTTATATATATAAATAAAGGAGTTTCAAAAGATTTTTTTTTTTTTTAGATTAATAAATAAACACATTACACTTGTTAAAATGTCCATTGTGTATATATATTCACATTACTGACATTTTATTGCTTATTTTATTTTGTATGTTTTCATATTTTTTTTATTTTTTTATTTTTTTGTAATAGAATTGCCAATCTACGCTTATGTTGATGTTGAATTCATGCATATGTGTGAAAATATCAATGAAAATATTGACATGTCAACATAAATTTAATACCATAATTATATTTAAATATTTTAATTCCTAAATTTTATAATCACAATTTGATCCCTTGGAGTATCTACTCGATCTATACATATATAAAAATCCTAGTAAATCTTAACTTCGTGTGATAAAGGATTAAATTGTTCCAAAATGTTAAAATAACATGGCTCAATTGTTTCAAAAATAATGTTTGAAACTTTAAACTATTATATACTTGAGTTCAGGAACTAATCCGTTGTTAGTTACAACGTTCACGAGTTAATATAATGCTTAAATACTTTAAAAACTAAGTCATCATTTCAATGAAATGTATATATATCGTTTTGGACAATTCTTCTATAAAGAAAAATTATTTGGTTAGTTAGAAAGTTAAATGCCTTTTTTAGGTGTTTTAATTTTAAGATATTTATACACTTGATCTTTTGAAATCACTTTGGCGAGTTGCACATCTCGATTTATGCTATCTACTCAAACAATATTTCTTTCTACTATTTAGTGTCCTCTTCTCTAGCTTGTACCTCTAACATACAATTATTAATGCTTTTAATTAGCTAAAAATACTTTTTGCCCCCACAATATAATGGGACACATATTCTACCTAGACAAATAGAAAATCACTTTTAACCCTTCCAAAATGCTTGTAAATTGAAGAACTTGTTATTATTTCACACTTTCTAAGTAAAAGCCTTTTCTTTTTTCTTCGATGATAATATGTTTAGAATGTTGAATCCGTGGAGTTCTTAGCTTCAATATTGAACGAGGACGAGGACGAAGAAATCATTGTAGAAGAAGCAATAGAAATAATTTCAAATATCAACTCACCCACCACTCTCCTCAAACACCTCCTAACCCAAAACTCTCAACTCATTGACACCCTAATCTCCATCATCACCATAACCAAAACCCCCAAATCTCGAGCCGCCACGATAGCCTTCCTTAGCTCCCTCTACGCCATTTCCGACCAAGCCCACAAAACCTTCACTAAGGACATCCTCTTCATCCAAATCATAAGAGCCTTAAAAGATCAAATCGCCACAAAGTCCACCCTCCAAATCCTCCTCCACTTGACCCCATTTGGCCGGAATCGCATCAAGGCCATAAACAATGGTGCTGTTTTCCACCTCGTCGAGCTGCTTCTAAACTCCTCTGGAAGAGAGTCCGAGCTGGCAATGGCAGTCCTGGACCGGCTTTGTGAATGCGCCGAAGGCCGTGCGGAGCTGCTTCGGCATGGGGGCGGGATGGCCGTGGTGTCGAGGAAGATTCTTAGAGTGTCGAATTTGGGCAATGAGAAGGCAGTGAGGATTGTGTACAATGTTAGCAAGAACAATGTTGGGAATTTTGGAGTTTTTGAGGAGATGGTGGAGGTTGGGGTTGTAGCGAAGCTTTGTTCGATGCTTCAAGTTTGTGGGAGTTTGAAGACTAAAGAAAGAATTAAGGAGATTCTTCATTTACTTCAATGTGTTTTCAAGGGTTCTAAGTGTGTTCTTGTTATTCCTTCTGGGTTTGATCGGTTTTAG

mRNA sequence

ATGACGGAAATCGAGGTTCCTTCAGACTTTTTGTGCCCTATTTCTCTACAGATCATGAGAGATCCGGTGACGATCTCGACGGGGATCACATACGACAGAGAGAGCATCGAGACATGGCTCTTGTGTTGCAATAACCGACCCGTTGTCGTCCTGAGCTGCCCAGTGACAAAGCAGGCGTTGAGCGGTATGGATTTGACTCCGAACCATACGCTACGGCGGGTGATTCAAGGATGGTGCAATCTCAATAGGAGGCATGGGGTGGAGCAGATCCCAACCCCTAAGGGTGAGGGTGATAGAGGAGATGTTGTTAAGTTGATTCTTAAGGAGGCAGTGAGAGGTCCAAGAAGCTCAAGGCTTGAGTGCTTAAAGAGATTGAAATCTTTTGTGGATGAGAGTGAGAGGAACAAAAATGTTGAATCCGTGGAGTTCTTAGCTTCAATATTGAACGAGGACGAGGACGAAGAAATCATTGTAGAAGAAGCAATAGAAATAATTTCAAATATCAACTCACCCACCACTCTCCTCAAACACCTCCTAACCCAAAACTCTCAACTCATTGACACCCTAATCTCCATCATCACCATAACCAAAACCCCCAAATCTCGAGCCGCCACGATAGCCTTCCTTAGCTCCCTCTACGCCATTTCCGACCAAGCCCACAAAACCTTCACTAAGGACATCCTCTTCATCCAAATCATAAGAGCCTTAAAAGATCAAATCGCCACAAAGTCCACCCTCCAAATCCTCCTCCACTTGACCCCATTTGGCCGGAATCGCATCAAGGCCATAAACAATGGTGCTGTTTTCCACCTCGTCGAGCTGCTTCTAAACTCCTCTGGAAGAGAGTCCGAGCTGGCAATGGCAGTCCTGGACCGGCTTTGTGAATGCGCCGAAGGCCGTGCGGAGCTGCTTCGGCATGGGGGCGGGATGGCCGTGGTGTCGAGGAAGATTCTTAGAGTGTCGAATTTGGGCAATGAGAAGGCAGTGAGGATTGTGTACAATGTTAGCAAGAACAATGTTGGGAATTTTGGAGTTTTTGAGGAGATGGTGGAGGTTGGGGTTGTAGCGAAGCTTTGTTCGATGCTTCAAGTTTGTGGGAGTTTGAAGACTAAAGAAAGAATTAAGGAGATTCTTCATTTACTTCAATGTGTTTTCAAGGGTTCTAAGTGTGTTCTTGTTATTCCTTCTGGGTTTGATCGGTTTTAG

Coding sequence (CDS)

ATGACGGAAATCGAGGTTCCTTCAGACTTTTTGTGCCCTATTTCTCTACAGATCATGAGAGATCCGGTGACGATCTCGACGGGGATCACATACGACAGAGAGAGCATCGAGACATGGCTCTTGTGTTGCAATAACCGACCCGTTGTCGTCCTGAGCTGCCCAGTGACAAAGCAGGCGTTGAGCGGTATGGATTTGACTCCGAACCATACGCTACGGCGGGTGATTCAAGGATGGTGCAATCTCAATAGGAGGCATGGGGTGGAGCAGATCCCAACCCCTAAGGGTGAGGGTGATAGAGGAGATGTTGTTAAGTTGATTCTTAAGGAGGCAGTGAGAGGTCCAAGAAGCTCAAGGCTTGAGTGCTTAAAGAGATTGAAATCTTTTGTGGATGAGAGTGAGAGGAACAAAAATGTTGAATCCGTGGAGTTCTTAGCTTCAATATTGAACGAGGACGAGGACGAAGAAATCATTGTAGAAGAAGCAATAGAAATAATTTCAAATATCAACTCACCCACCACTCTCCTCAAACACCTCCTAACCCAAAACTCTCAACTCATTGACACCCTAATCTCCATCATCACCATAACCAAAACCCCCAAATCTCGAGCCGCCACGATAGCCTTCCTTAGCTCCCTCTACGCCATTTCCGACCAAGCCCACAAAACCTTCACTAAGGACATCCTCTTCATCCAAATCATAAGAGCCTTAAAAGATCAAATCGCCACAAAGTCCACCCTCCAAATCCTCCTCCACTTGACCCCATTTGGCCGGAATCGCATCAAGGCCATAAACAATGGTGCTGTTTTCCACCTCGTCGAGCTGCTTCTAAACTCCTCTGGAAGAGAGTCCGAGCTGGCAATGGCAGTCCTGGACCGGCTTTGTGAATGCGCCGAAGGCCGTGCGGAGCTGCTTCGGCATGGGGGCGGGATGGCCGTGGTGTCGAGGAAGATTCTTAGAGTGTCGAATTTGGGCAATGAGAAGGCAGTGAGGATTGTGTACAATGTTAGCAAGAACAATGTTGGGAATTTTGGAGTTTTTGAGGAGATGGTGGAGGTTGGGGTTGTAGCGAAGCTTTGTTCGATGCTTCAAGTTTGTGGGAGTTTGAAGACTAAAGAAAGAATTAAGGAGATTCTTCATTTACTTCAATGTGTTTTCAAGGGTTCTAAGTGTGTTCTTGTTATTCCTTCTGGGTTTGATCGGTTTTAG

Protein sequence

MTEIEVPSDFLCPISLQIMRDPVTISTGITYDRESIETWLLCCNNRPVVVLSCPVTKQALSGMDLTPNHTLRRVIQGWCNLNRRHGVEQIPTPKGEGDRGDVVKLILKEAVRGPRSSRLECLKRLKSFVDESERNKNVESVEFLASILNEDEDEEIIVEEAIEIISNINSPTTLLKHLLTQNSQLIDTLISIITITKTPKSRAATIAFLSSLYAISDQAHKTFTKDILFIQIIRALKDQIATKSTLQILLHLTPFGRNRIKAINNGAVFHLVELLLNSSGRESELAMAVLDRLCECAEGRAELLRHGGGMAVVSRKILRVSNLGNEKAVRIVYNVSKNNVGNFGVFEEMVEVGVVAKLCSMLQVCGSLKTKERIKEILHLLQCVFKGSKCVLVIPSGFDRF
Homology
BLAST of Cla97C03G062600 vs. NCBI nr
Match: XP_038879749.1 (E3 ubiquitin-protein ligase PUB23-like isoform X1 [Benincasa hispida])

HSP 1 Score: 647.1 bits (1668), Expect = 9.8e-182
Identity = 344/406 (84.73%), Postives = 360/406 (88.67%), Query Frame = 0

Query: 1   MTEIEVPSDFLCPISLQIMRDPVTISTGITYDRESIETWLLCCNNRPVVVLSCPVTKQAL 60
           M EIEVPSDFLCPISLQIMRDPVTISTGITYDRESIE WL  C N     LSCPVTKQAL
Sbjct: 1   MAEIEVPSDFLCPISLQIMRDPVTISTGITYDRESIEKWLSSCKN-----LSCPVTKQAL 60

Query: 61  SGMDLTPNHTLRRVIQGWCNLNRRHGVEQIPTPKGEGDRGDVVKLILKEAVRGPRSSRLE 120
            G+DLTPNHTLRRVIQGWC+LN RHGVEQIPTP  E DRGDVVKLILKEAV+GPRSSRLE
Sbjct: 61  GGIDLTPNHTLRRVIQGWCSLNNRHGVEQIPTPNSEDDRGDVVKLILKEAVKGPRSSRLE 120

Query: 121 CLKRLKSFVDESERNK----NVESVEFLASILNEDEDEEIIVEEAIEIISNINSPTTLLK 180
           CLKRLK  V ESERNK    NVES+E LASIL EDEDE  IVEE IEIISNINS T LLK
Sbjct: 121 CLKRLKDIVAESERNKIYFQNVESMECLASILKEDEDEAAIVEETIEIISNINSSTALLK 180

Query: 181 HLLTQNSQLIDTLISIITITKTPKSRAATIAFLSSLYAISDQAHKTFTKDILFIQIIRAL 240
           H+LTQN +LIDTLISIITITKT KSRAATIAFLSSLY ISDQ HKTFTK+I+F+Q+ RAL
Sbjct: 181 HILTQNPRLIDTLISIITITKTSKSRAATIAFLSSLYTISDQTHKTFTKEIIFVQLTRAL 240

Query: 241 KDQIATKSTLQILLHLTPFGRNRIKAINNGAVFHLVELLLNSS-GRESELAMAVLDRLCE 300
           KDQIATK TL+ILLHL PFGRNRIKA+ NGAVFH+VELLL+SS GRE ELAMAVLDRLCE
Sbjct: 241 KDQIATKPTLKILLHLAPFGRNRIKAVKNGAVFHVVELLLHSSNGRECELAMAVLDRLCE 300

Query: 301 CAEGRAELLRHGGGMAVVSRKILRVSNLGNEKAVRIVYNVSKNNVGNFGVFEEMVEVGVV 360
           CAEGRAELLRHGGGMAVV RKILRVSNLGNEKAVRIVYNV KNNVGN GV EEMVEVGVV
Sbjct: 301 CAEGRAELLRHGGGMAVVGRKILRVSNLGNEKAVRIVYNVCKNNVGNLGVVEEMVEVGVV 360

Query: 361 AKLCSMLQVCGSLKTKERIKEILHLLQCVFKGSKCVLVIPSGFDRF 402
            KLC MLQV G+LKTKERIKEILHLLQCVFKG+KCVLVIPSGFDRF
Sbjct: 361 GKLCLMLQVGGNLKTKERIKEILHLLQCVFKGTKCVLVIPSGFDRF 401

BLAST of Cla97C03G062600 vs. NCBI nr
Match: XP_038879750.1 (E3 ubiquitin-protein ligase PUB23-like isoform X2 [Benincasa hispida])

HSP 1 Score: 618.6 bits (1594), Expect = 3.7e-173
Identity = 331/392 (84.44%), Postives = 346/392 (88.27%), Query Frame = 0

Query: 1   MTEIEVPSDFLCPISLQIMRDPVTISTGITYDRESIETWLLCCNNRPVVVLSCPVTKQAL 60
           M EIEVPSDFLCPISLQIMRDPVTISTGITYDRESIE WL  C N     LSCPVTKQAL
Sbjct: 1   MAEIEVPSDFLCPISLQIMRDPVTISTGITYDRESIEKWLSSCKN-----LSCPVTKQAL 60

Query: 61  SGMDLTPNHTLRRVIQGWCNLNRRHGVEQIPTPKGEGDRGDVVKLILKEAVRGPRSSRLE 120
            G+DLTPNHTLRRVIQGWC+LN RHGVEQIPTP  E DRGDVVKLILKEAV+GPRSSRLE
Sbjct: 61  GGIDLTPNHTLRRVIQGWCSLNNRHGVEQIPTPNSEDDRGDVVKLILKEAVKGPRSSRLE 120

Query: 121 CLKRLKSFVDESERNK----NVESVEFLASILNEDEDEEIIVEEAIEIISNINSPTTLLK 180
           CLKRLK  V ESERNK    NVES+E LASIL EDEDE  IVEE IEIISNINS T LLK
Sbjct: 121 CLKRLKDIVAESERNKIYFQNVESMECLASILKEDEDEAAIVEETIEIISNINSSTALLK 180

Query: 181 HLLTQNSQLIDTLISIITITKTPKSRAATIAFLSSLYAISDQAHKTFTKDILFIQIIRAL 240
           H+LTQN +LIDTLISIITITKT KSRAATIAFLSSLY ISDQ HKTFTK+I+F+Q+ RAL
Sbjct: 181 HILTQNPRLIDTLISIITITKTSKSRAATIAFLSSLYTISDQTHKTFTKEIIFVQLTRAL 240

Query: 241 KDQIATKSTLQILLHLTPFGRNRIKAINNGAVFHLVELLLNSS-GRESELAMAVLDRLCE 300
           KDQIATK TL+ILLHL PFGRNRIKA+ NGAVFH+VELLL+SS GRE ELAMAVLDRLCE
Sbjct: 241 KDQIATKPTLKILLHLAPFGRNRIKAVKNGAVFHVVELLLHSSNGRECELAMAVLDRLCE 300

Query: 301 CAEGRAELLRHGGGMAVVSRKILRVSNLGNEKAVRIVYNVSKNNVGNFGVFEEMVEVGVV 360
           CAEGRAELLRHGGGMAVV RKILRVSNLGNEKAVRIVYNV KNNVGN GV EEMVEVGVV
Sbjct: 301 CAEGRAELLRHGGGMAVVGRKILRVSNLGNEKAVRIVYNVCKNNVGNLGVVEEMVEVGVV 360

Query: 361 AKLCSMLQVCGSLKTKERIKEILHLLQCVFKG 388
            KLC MLQV G+LKTKERIKEILHLLQCVFKG
Sbjct: 361 GKLCLMLQVGGNLKTKERIKEILHLLQCVFKG 387

BLAST of Cla97C03G062600 vs. NCBI nr
Match: XP_004139144.2 (E3 ubiquitin-protein ligase PUB23 [Cucumis sativus] >KGN66593.1 hypothetical protein Csa_006907 [Cucumis sativus])

HSP 1 Score: 607.8 bits (1566), Expect = 6.6e-170
Identity = 325/410 (79.27%), Postives = 358/410 (87.32%), Query Frame = 0

Query: 1   MTEIEVPSDFLCPISLQIMRDPVTISTGITYDRESIETWLLCCNNRPVVVLSCPVTKQAL 60
           M EIEVPSDFLCPISLQ+MRDPVTISTGITYDRESI+ WL  C N+P   LSCPV+KQAL
Sbjct: 1   MAEIEVPSDFLCPISLQLMRDPVTISTGITYDRESIKKWLSSCKNQP---LSCPVSKQAL 60

Query: 61  SGMDLTPNHTLRRVIQGWCNLNRRHGVEQIPTPKGEGDRGDVVKLILKEAVRGPRSSRLE 120
           S +DLTPNHTLRRVIQGWC+LN RHGVEQIPTP+ E DRGDVVKLILKEA++ PRSS LE
Sbjct: 61  SSVDLTPNHTLRRVIQGWCSLNERHGVEQIPTPEDESDRGDVVKLILKEAMKSPRSSMLE 120

Query: 121 CLKRLKSFVDESERNK----NVESVEFLASIL----NEDEDEEIIVEEAIEIISNINSPT 180
           CLKRLKS V E+E NK    NVES+ FL SIL    +EDEDE+ +VEEAIEII++INSPT
Sbjct: 121 CLKRLKSIVAENESNKIYLQNVESMVFLTSILFEDEDEDEDEDAMVEEAIEIITDINSPT 180

Query: 181 TLLKHLLTQNSQLIDTLISIITITKTPKSRAATIAFLSSLYAISDQAHKTFTKDILFIQI 240
            +L ++LTQN QLIDTLISIIT TK+PKSRA+TIAFLSSLY ISDQ HKTFTKDILFIQ+
Sbjct: 181 IILNNVLTQNPQLIDTLISIITSTKSPKSRASTIAFLSSLYTISDQMHKTFTKDILFIQL 240

Query: 241 IRALKDQIATKSTLQILLHLTPFGRNRIKAINNGAVFHLVELLLNS-SGRESELAMAVLD 300
            RALKDQ+ATK  LQILL L PFGRNRIKA+ +G VF +VELLLNS +G+E ELAMAVLD
Sbjct: 241 TRALKDQVATKPALQILLRLAPFGRNRIKAVKHGVVFCVVELLLNSTNGKECELAMAVLD 300

Query: 301 RLCECAEGRAELLRHGGGMAVVSRKILRVSNLGNEKAVRIVYNVSKNNVGNFGVFEEMVE 360
           RLCECAEGRAELL+HGGGMAVV RKILRVSNLGNEKAVRI+YNV KNNVGN GV EEMVE
Sbjct: 301 RLCECAEGRAELLQHGGGMAVVGRKILRVSNLGNEKAVRILYNVCKNNVGNCGVAEEMVE 360

Query: 361 VGVVAKLCSMLQVCGSLKTKERIKEILHLLQCVFKGSKCVLVIPSGFDRF 402
           VGVV KLC MLQV G+LKTKERIKEILHLLQCVFKG+KCV V+PSGFDRF
Sbjct: 361 VGVVGKLCLMLQVGGNLKTKERIKEILHLLQCVFKGTKCV-VVPSGFDRF 406

BLAST of Cla97C03G062600 vs. NCBI nr
Match: XP_008450285.1 (PREDICTED: E3 ubiquitin-protein ligase PUB22-like [Cucumis melo])

HSP 1 Score: 604.7 bits (1558), Expect = 5.6e-169
Identity = 324/408 (79.41%), Postives = 355/408 (87.01%), Query Frame = 0

Query: 1   MTEIEVPSDFLCPISLQIMRDPVTISTGITYDRESIETWLLCCNNRPVVVLSCPVTKQAL 60
           M EIEVPSDFLCPISLQIMRDPVTISTGITYDRE I+ WL  C N+P++   CPV+KQAL
Sbjct: 1   MAEIEVPSDFLCPISLQIMRDPVTISTGITYDRECIKKWLSSCKNQPLI---CPVSKQAL 60

Query: 61  SGMDLTPNHTLRRVIQGWCNLNRRHGVEQIPTPKGEGDRGDVVKLILKEAVRGPRSSRLE 120
           S +DLTPNHTL RVIQGWC+L+ RHGVEQIPTPK E DRGD VK ILKEA++ PRSSRLE
Sbjct: 61  SSVDLTPNHTLGRVIQGWCSLHERHGVEQIPTPKDEIDRGDAVKSILKEAMKSPRSSRLE 120

Query: 121 CLKRLKSFVDESERNK----NVESVEFLASILNED--EDEEIIVEEAIEIISNINSPTTL 180
           CLKRLKS V E+E NK    NVES+ FL SIL ED  EDE+++VEEAIEII+NINSP T+
Sbjct: 121 CLKRLKSIVAENESNKIYLQNVESMVFLTSILFEDEGEDEDVMVEEAIEIITNINSPATI 180

Query: 181 LKHLLTQNSQLIDTLISIITITKTPKSRAATIAFLSSLYAISDQAHKTFTKDILFIQIIR 240
           LK+LLTQN QLIDTLISIIT TK+PKSRA+TIAFL SLY ISDQ HKTFTKDILFIQ+ R
Sbjct: 181 LKNLLTQNPQLIDTLISIITSTKSPKSRASTIAFLCSLYTISDQTHKTFTKDILFIQLTR 240

Query: 241 ALKDQIATKSTLQILLHLTPFGRNRIKAINNGAVFHLVELLLNSS-GRESELAMAVLDRL 300
           AL DQI+TK  LQILL L PFGRNRIKAI NG VFH+VELLLNSS GRE ELAMAVLDRL
Sbjct: 241 ALMDQISTKPALQILLRLAPFGRNRIKAIKNGVVFHVVELLLNSSNGRECELAMAVLDRL 300

Query: 301 CECAEGRAELLRHGGGMAVVSRKILRVSNLGNEKAVRIVYNVSKNNVGNFGVFEEMVEVG 360
           CECAEGRAELL+HGGGMAVV RKILRVSNLGNEKAVRI+YNV KNNVGN GV EEMVEVG
Sbjct: 301 CECAEGRAELLQHGGGMAVVGRKILRVSNLGNEKAVRILYNVCKNNVGNCGVAEEMVEVG 360

Query: 361 VVAKLCSMLQVCGSLKTKERIKEILHLLQCVFKGSKCVLVIPSGFDRF 402
           VVAKLC MLQV G+LKT+ER+KEILHLLQCV +G+KCV V+PSGFDRF
Sbjct: 361 VVAKLCLMLQVGGNLKTRERVKEILHLLQCVSRGTKCV-VVPSGFDRF 404

BLAST of Cla97C03G062600 vs. NCBI nr
Match: XP_022961129.1 (E3 ubiquitin-protein ligase PUB23-like [Cucurbita moschata])

HSP 1 Score: 570.9 bits (1470), Expect = 8.9e-159
Identity = 303/408 (74.26%), Postives = 344/408 (84.31%), Query Frame = 0

Query: 1   MTEIEVPSDFLCPISLQIMRDPVTISTGITYDRESIETWLLCCNNRPVVVLSCPVTKQAL 60
           M EIEVPSDFLCPISLQIM DPVTISTGITYDRESIE WLL C N       CPVTKQ L
Sbjct: 1   MPEIEVPSDFLCPISLQIMSDPVTISTGITYDRESIEKWLLFCKN-------CPVTKQPL 60

Query: 61  SGMDLTPNHTLRRVIQGWCNLNRRHGVEQIPTPKGEGDRGDVVKLILKEAVRGPRSSRLE 120
            G DLTPNHTLRRVIQGWC +N  HG E++ +PKGEGD G VVK+ILKEA+RG RSSRLE
Sbjct: 61  IGTDLTPNHTLRRVIQGWCCVNAEHGAERVRSPKGEGDGGHVVKVILKEAMRG-RSSRLE 120

Query: 121 CLKRLKSFVDESERNK----NVESVEFLASILN--EDEDEEIIVEEAIEIISNINSPTTL 180
           CL +L++F+ E+ERNK    +  S+EFLAS+LN  ED +E++IVE+AIEIISNINSPTTL
Sbjct: 121 CLNKLEAFLAETERNKIYLQDARSMEFLASMLNNPEDGNEDVIVEKAIEIISNINSPTTL 180

Query: 181 LKHLLTQNSQLIDTLISIITITKTPKSRAATIAFLSSLYAISDQAHKTFTKDILFIQIIR 240
           L+HLL +NS LID +ISI+ +TKT +SRAA IAFLSSLY+ISDQ HKTFTKD LFIQ+ R
Sbjct: 181 LEHLLIRNSNLIDVVISIVAVTKTSRSRAAAIAFLSSLYSISDQTHKTFTKDDLFIQLSR 240

Query: 241 ALKDQIATKSTLQILLHLTPFGRNRIKAINNGAVFHLVELLLNSS-GRESELAMAVLDRL 300
           AL DQIA K TL+ILLHL PFGRNRIKA+ +G VF LVELLLNS+ GRE ELA+  +D+L
Sbjct: 241 ALNDQIAIKPTLKILLHLAPFGRNRIKAVKHGVVFDLVELLLNSTVGRECELAIMAMDQL 300

Query: 301 CECAEGRAELLRHGGGMAVVSRKILRVSNLGNEKAVRIVYNVSKNNVGNFGVFEEMVEVG 360
           CECAEGRAELLRHGGGMAVV+RKILRVSN  NEKAVRI+Y+V KNNVGNFGV EEMVEVG
Sbjct: 301 CECAEGRAELLRHGGGMAVVARKILRVSNFSNEKAVRILYHVCKNNVGNFGVAEEMVEVG 360

Query: 361 VVAKLCSMLQVCGSLKTKERIKEILHLLQCVFKGSKCVLVIPSGFDRF 402
            V KLC MLQ+ GSLKT ERIKEILH L+C+FKG+KCVLV+PSGFDRF
Sbjct: 361 AVGKLCLMLQIGGSLKTNERIKEILHFLRCIFKGTKCVLVLPSGFDRF 400

BLAST of Cla97C03G062600 vs. ExPASy Swiss-Prot
Match: Q84TG3 (E3 ubiquitin-protein ligase PUB23 OS=Arabidopsis thaliana OX=3702 GN=PUB23 PE=1 SV=1)

HSP 1 Score: 288.1 bits (736), Expect = 1.5e-76
Identity = 170/402 (42.29%), Postives = 256/402 (63.68%), Query Frame = 0

Query: 3   EIEVPSDFLCPISLQIMRDPVTISTGITYDRESIETWLLCCNNRPVVVLSCPVTKQALSG 62
           EIE+P  FLCPISL+IM+DPV +STGITYDR+SIE WL           SCPVTKQ ++ 
Sbjct: 9   EIEIPPFFLCPISLEIMKDPVIVSTGITYDRDSIEKWLFAGKKN-----SCPVTKQDITD 68

Query: 63  MDLTPNHTLRRVIQGWCNLNRRHGVEQIPTPKGEGDRGDVVKLILKEAVRGPRSSRLECL 122
            DLTPNHTLRR+IQ WC LN  +GVE+IPTP+    + ++ KLI   A      ++++CL
Sbjct: 69  ADLTPNHTLRRLIQSWCTLNASYGVERIPTPRPPICKSEIEKLIRDSA--SSHENQVKCL 128

Query: 123 KRLKSFVDESERNKN-VESV---EFLASILNEDEDEEIIVEEAIEIISNINSPTTLLKHL 182
           KRL+  V E+  NK  +E+    EFLA+I++ D +   + +EA+ ++ ++ +  T+LK+L
Sbjct: 129 KRLRQIVSENATNKRCLEAAGVPEFLANIVSNDSENGSLTDEALNLLYHLETSETVLKNL 188

Query: 183 LT--QNSQLIDTLISIITITKTPKSRAATIAFLSSLYAISDQAHKTFTKDILFIQIIRAL 242
           L   +++ ++ +L  I+      +SR      L ++  ++D       K  +F ++++ L
Sbjct: 189 LNNKKDNNIVKSLTKIMQ-RGMYESRVYATLLLKNILEVADPMQSMTLKPEVFTEVVQIL 248

Query: 243 KDQI---ATKSTLQILLHLTPFGRNRIKAINNGAVFHLVELLLN----SSGRESELAMAV 302
            D+I   ATK+ + IL+++ P+GRNR KA+  G +  ++ELL++    S  R  E+AM V
Sbjct: 249 DDRISQKATKAAMHILVNICPWGRNRHKAVEAGVISVIIELLMDESFTSERRGPEMAMVV 308

Query: 303 LDRLCECAEGRAELLRHGGGMAVVSRKILRVSNLGNEKAVRIVYNVSKNNVGNFGVFEEM 362
           LD LC+CAEGRAE L HG  +AVV +KILRVS   +++AVR++ +V +       +  EM
Sbjct: 309 LDLLCQCAEGRAEFLNHGAAIAVVCKKILRVSQTASDRAVRVLLSVGR-FCATPALLHEM 368

Query: 363 VEVGVVAKLCSMLQVCGSLKTKERIKEILHLLQCVFKGSKCV 392
           +++GVVAKLC +LQV    KTKE+ KE+L L   V+K S C+
Sbjct: 369 LQLGVVAKLCLVLQVSCGGKTKEKAKELLKLHARVWKDSPCL 401

BLAST of Cla97C03G062600 vs. ExPASy Swiss-Prot
Match: Q9SVC6 (E3 ubiquitin-protein ligase PUB22 OS=Arabidopsis thaliana OX=3702 GN=PUB22 PE=1 SV=1)

HSP 1 Score: 286.6 bits (732), Expect = 4.4e-76
Identity = 181/431 (42.00%), Postives = 256/431 (59.40%), Query Frame = 0

Query: 3   EIEVPSDFLCPISLQIMRDPVTISTGITYDRESIETWLLCCNNRPVVVLSCPVTKQALSG 62
           EIE+PS FLCPISL IM+DPV +STGITYDRESIE WL           SCPVTKQ ++ 
Sbjct: 4   EIEIPSFFLCPISLDIMKDPVIVSTGITYDRESIEKWLFSGKKN-----SCPVTKQVITE 63

Query: 63  MDLTPNHTLRRVIQGWCNLNRRHGVEQIPTPKGEGDRGDVVKLILKEAVRGPRSSRLECL 122
            DLTPNHTLRR+IQ WC LN  +G+E+IPTPK    + ++ KLI + +      ++++CL
Sbjct: 64  TDLTPNHTLRRLIQSWCTLNASYGIERIPTPKPPICKSEIEKLIKESS--SSHLNQVKCL 123

Query: 123 KRLKSFVDESERNKN----VESVEFLASIL-----------------------------N 182
           KRL+  V E+  NK      E  EFLA+I+                             N
Sbjct: 124 KRLRQIVSENTTNKRCLEAAEVPEFLANIVSNSVDTYNSPSSSLSSSNLNDMCQSNMLEN 183

Query: 183 EDEDEEIIVEEAIEIISNINSPTTLLKHLLT--QNSQLIDTLISIITITKTPKSRAATIA 242
             +    +++EA+ ++ ++++  T LK LL   + + L+ TL  I+      +SRA    
Sbjct: 184 RFDSSRSLMDEALSVLYHLDTSETALKSLLNNKKGTNLVKTLTKIMQ-RGIYESRAYAAL 243

Query: 243 FLSSLYAISDQAHKTFTKDILFIQIIRALKDQI---ATKSTLQILLHLTPFGRNRIKAIN 302
            L  L  ++D       +  LF ++I+ L DQI   AT+S +QIL+   P+GRNR KA+ 
Sbjct: 244 LLKKLLEVADPMQIILLERELFGEVIQILHDQISHKATRSAMQILVITCPWGRNRHKAVE 303

Query: 303 NGAVFHLVELLL----NSSGRESELAMAVLDRLCECAEGRAELLRHGGGMAVVSRKILRV 362
            G +  ++ELL+    +S  R SE+AM VLD LC+CAEGRAE L HG  +AVVS+KILRV
Sbjct: 304 GGTISMIIELLMDDTFSSERRNSEMAMVVLDMLCQCAEGRAEFLNHGAAIAVVSKKILRV 363

Query: 363 SNLGNEKAVRIVYNVSKNNVGNFGVFEEMVEVGVVAKLCSMLQVCGSLKTKERIKEILHL 392
           S + +E+AVR++ +V +       + +EM+++GVVAKLC +LQV    KTKE+ KE+L L
Sbjct: 364 SQITSERAVRVLLSVGR-FCATPSLLQEMLQLGVVAKLCLVLQVSCGNKTKEKAKELLKL 423

BLAST of Cla97C03G062600 vs. ExPASy Swiss-Prot
Match: Q9SF15 (E3 ubiquitin-protein ligase PUB24 OS=Arabidopsis thaliana OX=3702 GN=PUB24 PE=1 SV=1)

HSP 1 Score: 194.1 bits (492), Expect = 3.0e-48
Identity = 154/428 (35.98%), Postives = 225/428 (52.57%), Query Frame = 0

Query: 3   EIEVPSDFLCPISLQIMRDPVTISTGITYDRESIETWLLCCNNRPVVVLSCPVTKQALS- 62
           EIE+P+ F+CPISL+IM+DPVT  +GITYDR++I  WL         V SCPVTKQ L  
Sbjct: 7   EIEIPNYFICPISLEIMKDPVTTVSGITYDRQNIVKWL-------EKVPSCPVTKQPLPL 66

Query: 63  GMDLTPNHTLRRVIQGWCNLNRRHGVEQIPTPKGEGDRGDVVKLILKEAVRGPRS-SRLE 122
             DLTPNH LRR+IQ WC  N   GV +I TP+    + +VV+ I      G  +  R E
Sbjct: 67  DSDLTPNHMLRRLIQHWCVENETRGVVRISTPRVPPGKLNVVEEIKNLKKFGQEALGREE 126

Query: 123 CLKRLKSF-VDESERNKNVES------VEFLASILNEDEDEEIIV---EEAIEIISNINS 182
            L++L+   +D + R    E       + F+    +EDED    +   +E++ ++  I  
Sbjct: 127 TLQKLEVLAMDGNNRRLMCECGVHKSLILFVVKCTSEDEDGRRRIKGLDESLRLLHLIGI 186

Query: 183 PTTLLKHLLTQNSQLIDTLISIITITKTPKSRAATIAFLSSLYAISDQAHKTFTKDILFI 242
           P+   K +L +N +++++L  ++   +   S+A TI  L +L   +           +F 
Sbjct: 187 PSNDAKTILMENDRVMESLTWVLH-QEDFLSKAYTIVLLRNLTEYTSSHIVERLNPEIFK 246

Query: 243 QIIRALKDQI--------------------------------------ATKSTLQILLHL 302
            II  LKD +                                      A  + L ILL  
Sbjct: 247 GIIGFLKDVVNSVNRTSPTVRETVQSSSRPSLGKTEPSKLDHSLVIKQAVTAALMILLET 306

Query: 303 TPFGRNRIKAINNGAVFHLVELLLNSSG--RESELAMAVLDRLCECAEGRAELLRHGGGM 362
           + + RNR   ++ GAV  L+EL ++ +G  R +EL + VL RLC CA GRAE+L H GG+
Sbjct: 307 SSWSRNRSLLVDLGAVSELIELEISYTGEKRITELMLGVLSRLCCCANGRAEILAHRGGI 366

Query: 363 AVVSRKILRVSNLGNEKAVRIVYNVSKNNVGNFGVFEEMVEVGVVAKLCSMLQVCGSLKT 379
           AVV++++LRVS   +++A+ I+  VSK +  N  V EEMV VG V KLCS+L +   L  
Sbjct: 367 AVVTKRLLRVSPAADDRAISILTTVSKFSPENM-VVEEMVNVGTVEKLCSVLGMDCGLNL 425

BLAST of Cla97C03G062600 vs. ExPASy Swiss-Prot
Match: Q9LT79 (U-box domain-containing protein 25 OS=Arabidopsis thaliana OX=3702 GN=PUB25 PE=2 SV=1)

HSP 1 Score: 149.1 bits (375), Expect = 1.1e-34
Identity = 126/396 (31.82%), Postives = 204/396 (51.52%), Query Frame = 0

Query: 4   IEVPSDFLCPISLQIMRDPVTISTGITYDRESIETWLLCCNNRPVVVLSCPVTKQALSGM 63
           I++P  F CPISL++M+DPVT+ TG TYDR SIE+W+   NN      +CPVT+  LS  
Sbjct: 12  IQIPYHFRCPISLELMQDPVTVCTGQTYDRASIESWVSIGNN-----TTCPVTRAPLSDF 71

Query: 64  DLTPNHTLRRVIQGWCNLNRRHGVEQIPTPKGEGDRGDVVKLILK-EAVRGPR---SSRL 123
            L PNHTLRR+IQ WC  NR +GVE+IPTPK   D   V  L+ +  A+ G      SR 
Sbjct: 72  TLIPNHTLRRLIQEWCVANRSNGVERIPTPKQPADPTSVRALLSQASAITGTHVSVRSRA 131

Query: 124 ECLKRLKSFVDESERNKNV----ESVEFLASIL-NEDEDEEIIVEE-AIEIISNINSPTT 183
             L+RL+ F  +S++N+ +     + E L  IL +E    E++ E  A+ ++  I  P  
Sbjct: 132 AALRRLRGFARDSDKNRVLIAAHNATEILIKILFSETTSSELVSESLALLVMLPITEPNQ 191

Query: 184 LLKHLLTQNSQLIDTLISII--TITKTPKSRAATIAFLSSLYAISDQAHKTFTKDILFIQ 243
            +   ++ +   ++ L  ++  +  +T  + AA I  +S+    +D        + +F  
Sbjct: 192 FVS--ISSDPGRVEFLTRLLFDSSIETRVNAAALIEIVSTGTKSADLKGSISNSESVFEG 251

Query: 244 IIRALKDQIATKSTLQI----LLHLTPFGRNRIKAINNGAVFHLVELLLNSSGR-ESELA 303
           ++  L++ I+++  L+I    L  L      R  AI  GA   L++ L     R ++E A
Sbjct: 252 VLDLLRNPISSRRALKIGIKTLFALCSVKSTRHIAITAGAPEILIDRLAADFDRCDTERA 311

Query: 304 MAVLDRLCECAEGRAELLRHGGGMAVVSRKILRVSNLGNEKAVRIVYNVSKNNVGNFGVF 363
           +A ++ LC   EG A    H   + ++ + ILRVS+   E A   +  +           
Sbjct: 312 LATVELLCRTPEGCAAFGEHALTVPLLVKTILRVSDRATEYAAGALLALC---TAEERWR 371

Query: 364 EEMVEVGVVAKLCSMLQVCGSLKTKERIKEILHLLQ 383
           EE    GVV +L  M+Q   + + K++ +++L LL+
Sbjct: 372 EEAAGAGVVVQLLLMVQSECTERAKKKAQKLLKLLR 397

BLAST of Cla97C03G062600 vs. ExPASy Swiss-Prot
Match: Q9C8D1 (U-box domain-containing protein 20 OS=Arabidopsis thaliana OX=3702 GN=PUB20 PE=1 SV=1)

HSP 1 Score: 138.3 bits (347), Expect = 1.9e-31
Identity = 113/409 (27.63%), Postives = 199/409 (48.66%), Query Frame = 0

Query: 3   EIEVPSDFLCPISLQIMRDPVTISTGITYDRESIETWLLCCNNRPVVVLSCPVTKQALSG 62
           EI +PS F CPIS ++M+DPV I++GITYDRE+IE W            +CPVT   L+ 
Sbjct: 30  EITIPSQFQCPISYELMKDPVIIASGITYDRENIEKWF------ESGYQTCPVTNTVLTS 89

Query: 63  MDLTPNHTLRRVIQGWCNLNRRHGVEQIPTPK---GEGDRGDVVKLILKEAVRGPRSSRL 122
           ++  PNHT+RR+IQGWC  +   G+E+IPTP+         ++ + +     RG  ++ +
Sbjct: 90  LEQIPNHTIRRMIQGWCGSSLGGGIERIPTPRVPVTSHQVSEICERLSAATRRGDYAACM 149

Query: 123 ECLKRLKSFVDESERNKNVESVEFLASIL-------NEDEDEEIIVEEAIEIISNINSPT 182
           E + ++     ESERN+          +L       +E+ +  +++EE + ++      T
Sbjct: 150 EMVTKMTRLGKESERNRKCVKENGAGLVLCVCFDAFSENANASLLLEETVSVL------T 209

Query: 183 TLLKHLLTQNSQLIDT-----LISII---------TITKTPKSRAATIAFLSSLYAISDQ 242
            +L   L   S+L  T     L+ ++          I +  +     +  L+ +  + + 
Sbjct: 210 WMLPIGLEGQSKLTTTSSFNRLVELLRNGDQNAAFLIKELLELNVTHVHALTKINGVQEA 269

Query: 243 AHKTFTKDILFIQIIRALKDQIAT-KSTLQILLHLTPFGRNRIKAINNGAVFHLVELLLN 302
             K+  +D   +  + ++   I T + T+   L L               V   VE+L++
Sbjct: 270 FMKSINRDSTCVNSLISIHHMILTNQETVSRFLEL-------------DLVNITVEMLVD 329

Query: 303 SSGRESELAMAVLDRLCECAEGRAELLRHGGGMAVVSRKILRVSNLGNEKAVRIVYNVSK 362
           S     E A+ VL+ +CE  EGR ++ R+   + ++ +KIL++S    +  V +++ V K
Sbjct: 330 SENSVCEKALTVLNVICETKEGREKVRRNKLVIPILVKKILKISE--KKDLVSVMWKVCK 389

Query: 363 NNVGNFGVFEEMVEVGVVAKLCSMLQVCGSLKTKERIKEILHLLQCVFK 387
           +  G+    EE + +G   KL  MLQV     TKE++ E+L ++  V K
Sbjct: 390 S--GDGSEVEEALRLGAFKKLVVMLQVGCGEGTKEKVTELLKMMNKVMK 409

BLAST of Cla97C03G062600 vs. ExPASy TrEMBL
Match: A0A0A0LXW1 (RING-type E3 ubiquitin transferase OS=Cucumis sativus OX=3659 GN=Csa_1G635930 PE=4 SV=1)

HSP 1 Score: 607.8 bits (1566), Expect = 3.2e-170
Identity = 325/410 (79.27%), Postives = 358/410 (87.32%), Query Frame = 0

Query: 1   MTEIEVPSDFLCPISLQIMRDPVTISTGITYDRESIETWLLCCNNRPVVVLSCPVTKQAL 60
           M EIEVPSDFLCPISLQ+MRDPVTISTGITYDRESI+ WL  C N+P   LSCPV+KQAL
Sbjct: 1   MAEIEVPSDFLCPISLQLMRDPVTISTGITYDRESIKKWLSSCKNQP---LSCPVSKQAL 60

Query: 61  SGMDLTPNHTLRRVIQGWCNLNRRHGVEQIPTPKGEGDRGDVVKLILKEAVRGPRSSRLE 120
           S +DLTPNHTLRRVIQGWC+LN RHGVEQIPTP+ E DRGDVVKLILKEA++ PRSS LE
Sbjct: 61  SSVDLTPNHTLRRVIQGWCSLNERHGVEQIPTPEDESDRGDVVKLILKEAMKSPRSSMLE 120

Query: 121 CLKRLKSFVDESERNK----NVESVEFLASIL----NEDEDEEIIVEEAIEIISNINSPT 180
           CLKRLKS V E+E NK    NVES+ FL SIL    +EDEDE+ +VEEAIEII++INSPT
Sbjct: 121 CLKRLKSIVAENESNKIYLQNVESMVFLTSILFEDEDEDEDEDAMVEEAIEIITDINSPT 180

Query: 181 TLLKHLLTQNSQLIDTLISIITITKTPKSRAATIAFLSSLYAISDQAHKTFTKDILFIQI 240
            +L ++LTQN QLIDTLISIIT TK+PKSRA+TIAFLSSLY ISDQ HKTFTKDILFIQ+
Sbjct: 181 IILNNVLTQNPQLIDTLISIITSTKSPKSRASTIAFLSSLYTISDQMHKTFTKDILFIQL 240

Query: 241 IRALKDQIATKSTLQILLHLTPFGRNRIKAINNGAVFHLVELLLNS-SGRESELAMAVLD 300
            RALKDQ+ATK  LQILL L PFGRNRIKA+ +G VF +VELLLNS +G+E ELAMAVLD
Sbjct: 241 TRALKDQVATKPALQILLRLAPFGRNRIKAVKHGVVFCVVELLLNSTNGKECELAMAVLD 300

Query: 301 RLCECAEGRAELLRHGGGMAVVSRKILRVSNLGNEKAVRIVYNVSKNNVGNFGVFEEMVE 360
           RLCECAEGRAELL+HGGGMAVV RKILRVSNLGNEKAVRI+YNV KNNVGN GV EEMVE
Sbjct: 301 RLCECAEGRAELLQHGGGMAVVGRKILRVSNLGNEKAVRILYNVCKNNVGNCGVAEEMVE 360

Query: 361 VGVVAKLCSMLQVCGSLKTKERIKEILHLLQCVFKGSKCVLVIPSGFDRF 402
           VGVV KLC MLQV G+LKTKERIKEILHLLQCVFKG+KCV V+PSGFDRF
Sbjct: 361 VGVVGKLCLMLQVGGNLKTKERIKEILHLLQCVFKGTKCV-VVPSGFDRF 406

BLAST of Cla97C03G062600 vs. ExPASy TrEMBL
Match: A0A1S3BNV9 (RING-type E3 ubiquitin transferase OS=Cucumis melo OX=3656 GN=LOC103491935 PE=4 SV=1)

HSP 1 Score: 604.7 bits (1558), Expect = 2.7e-169
Identity = 324/408 (79.41%), Postives = 355/408 (87.01%), Query Frame = 0

Query: 1   MTEIEVPSDFLCPISLQIMRDPVTISTGITYDRESIETWLLCCNNRPVVVLSCPVTKQAL 60
           M EIEVPSDFLCPISLQIMRDPVTISTGITYDRE I+ WL  C N+P++   CPV+KQAL
Sbjct: 1   MAEIEVPSDFLCPISLQIMRDPVTISTGITYDRECIKKWLSSCKNQPLI---CPVSKQAL 60

Query: 61  SGMDLTPNHTLRRVIQGWCNLNRRHGVEQIPTPKGEGDRGDVVKLILKEAVRGPRSSRLE 120
           S +DLTPNHTL RVIQGWC+L+ RHGVEQIPTPK E DRGD VK ILKEA++ PRSSRLE
Sbjct: 61  SSVDLTPNHTLGRVIQGWCSLHERHGVEQIPTPKDEIDRGDAVKSILKEAMKSPRSSRLE 120

Query: 121 CLKRLKSFVDESERNK----NVESVEFLASILNED--EDEEIIVEEAIEIISNINSPTTL 180
           CLKRLKS V E+E NK    NVES+ FL SIL ED  EDE+++VEEAIEII+NINSP T+
Sbjct: 121 CLKRLKSIVAENESNKIYLQNVESMVFLTSILFEDEGEDEDVMVEEAIEIITNINSPATI 180

Query: 181 LKHLLTQNSQLIDTLISIITITKTPKSRAATIAFLSSLYAISDQAHKTFTKDILFIQIIR 240
           LK+LLTQN QLIDTLISIIT TK+PKSRA+TIAFL SLY ISDQ HKTFTKDILFIQ+ R
Sbjct: 181 LKNLLTQNPQLIDTLISIITSTKSPKSRASTIAFLCSLYTISDQTHKTFTKDILFIQLTR 240

Query: 241 ALKDQIATKSTLQILLHLTPFGRNRIKAINNGAVFHLVELLLNSS-GRESELAMAVLDRL 300
           AL DQI+TK  LQILL L PFGRNRIKAI NG VFH+VELLLNSS GRE ELAMAVLDRL
Sbjct: 241 ALMDQISTKPALQILLRLAPFGRNRIKAIKNGVVFHVVELLLNSSNGRECELAMAVLDRL 300

Query: 301 CECAEGRAELLRHGGGMAVVSRKILRVSNLGNEKAVRIVYNVSKNNVGNFGVFEEMVEVG 360
           CECAEGRAELL+HGGGMAVV RKILRVSNLGNEKAVRI+YNV KNNVGN GV EEMVEVG
Sbjct: 301 CECAEGRAELLQHGGGMAVVGRKILRVSNLGNEKAVRILYNVCKNNVGNCGVAEEMVEVG 360

Query: 361 VVAKLCSMLQVCGSLKTKERIKEILHLLQCVFKGSKCVLVIPSGFDRF 402
           VVAKLC MLQV G+LKT+ER+KEILHLLQCV +G+KCV V+PSGFDRF
Sbjct: 361 VVAKLCLMLQVGGNLKTRERVKEILHLLQCVSRGTKCV-VVPSGFDRF 404

BLAST of Cla97C03G062600 vs. ExPASy TrEMBL
Match: A0A6J1HD28 (RING-type E3 ubiquitin transferase OS=Cucurbita moschata OX=3662 GN=LOC111461732 PE=4 SV=1)

HSP 1 Score: 570.9 bits (1470), Expect = 4.3e-159
Identity = 303/408 (74.26%), Postives = 344/408 (84.31%), Query Frame = 0

Query: 1   MTEIEVPSDFLCPISLQIMRDPVTISTGITYDRESIETWLLCCNNRPVVVLSCPVTKQAL 60
           M EIEVPSDFLCPISLQIM DPVTISTGITYDRESIE WLL C N       CPVTKQ L
Sbjct: 1   MPEIEVPSDFLCPISLQIMSDPVTISTGITYDRESIEKWLLFCKN-------CPVTKQPL 60

Query: 61  SGMDLTPNHTLRRVIQGWCNLNRRHGVEQIPTPKGEGDRGDVVKLILKEAVRGPRSSRLE 120
            G DLTPNHTLRRVIQGWC +N  HG E++ +PKGEGD G VVK+ILKEA+RG RSSRLE
Sbjct: 61  IGTDLTPNHTLRRVIQGWCCVNAEHGAERVRSPKGEGDGGHVVKVILKEAMRG-RSSRLE 120

Query: 121 CLKRLKSFVDESERNK----NVESVEFLASILN--EDEDEEIIVEEAIEIISNINSPTTL 180
           CL +L++F+ E+ERNK    +  S+EFLAS+LN  ED +E++IVE+AIEIISNINSPTTL
Sbjct: 121 CLNKLEAFLAETERNKIYLQDARSMEFLASMLNNPEDGNEDVIVEKAIEIISNINSPTTL 180

Query: 181 LKHLLTQNSQLIDTLISIITITKTPKSRAATIAFLSSLYAISDQAHKTFTKDILFIQIIR 240
           L+HLL +NS LID +ISI+ +TKT +SRAA IAFLSSLY+ISDQ HKTFTKD LFIQ+ R
Sbjct: 181 LEHLLIRNSNLIDVVISIVAVTKTSRSRAAAIAFLSSLYSISDQTHKTFTKDDLFIQLSR 240

Query: 241 ALKDQIATKSTLQILLHLTPFGRNRIKAINNGAVFHLVELLLNSS-GRESELAMAVLDRL 300
           AL DQIA K TL+ILLHL PFGRNRIKA+ +G VF LVELLLNS+ GRE ELA+  +D+L
Sbjct: 241 ALNDQIAIKPTLKILLHLAPFGRNRIKAVKHGVVFDLVELLLNSTVGRECELAIMAMDQL 300

Query: 301 CECAEGRAELLRHGGGMAVVSRKILRVSNLGNEKAVRIVYNVSKNNVGNFGVFEEMVEVG 360
           CECAEGRAELLRHGGGMAVV+RKILRVSN  NEKAVRI+Y+V KNNVGNFGV EEMVEVG
Sbjct: 301 CECAEGRAELLRHGGGMAVVARKILRVSNFSNEKAVRILYHVCKNNVGNFGVAEEMVEVG 360

Query: 361 VVAKLCSMLQVCGSLKTKERIKEILHLLQCVFKGSKCVLVIPSGFDRF 402
            V KLC MLQ+ GSLKT ERIKEILH L+C+FKG+KCVLV+PSGFDRF
Sbjct: 361 AVGKLCLMLQIGGSLKTNERIKEILHFLRCIFKGTKCVLVLPSGFDRF 400

BLAST of Cla97C03G062600 vs. ExPASy TrEMBL
Match: A0A6J1JH48 (RING-type E3 ubiquitin transferase OS=Cucurbita maxima OX=3661 GN=LOC111485066 PE=4 SV=1)

HSP 1 Score: 562.8 bits (1449), Expect = 1.2e-156
Identity = 300/408 (73.53%), Postives = 339/408 (83.09%), Query Frame = 0

Query: 1   MTEIEVPSDFLCPISLQIMRDPVTISTGITYDRESIETWLLCCNNRPVVVLSCPVTKQAL 60
           M EIEVPSDFLCPISLQIM DPVTISTGITYDRESIE WLL C         CPVTKQ L
Sbjct: 1   MAEIEVPSDFLCPISLQIMSDPVTISTGITYDRESIEKWLLFCK-------KCPVTKQPL 60

Query: 61  SGMDLTPNHTLRRVIQGWCNLNRRHGVEQIPTPKGEGDRGDVVKLILKEAVRGPRSSRLE 120
            G+DLTPNHTLRRVIQGW  +N  HGVE++ +PKGEGD G VVKLILKEA+RG RSSRLE
Sbjct: 61  IGIDLTPNHTLRRVIQGWSCVNTEHGVERVRSPKGEGDGGHVVKLILKEAMRG-RSSRLE 120

Query: 121 CLKRLKSFVDESERNK----NVESVEFLASILN--EDEDEEIIVEEAIEIISNINSPTTL 180
           CL +L++F+ E+ERNK    +  S+EFL S+LN  EDE+EE+IVE+AIEII NINSPTTL
Sbjct: 121 CLNKLEAFLAETERNKIYLQDARSMEFLTSMLNNPEDENEEVIVEKAIEIILNINSPTTL 180

Query: 181 LKHLLTQNSQLIDTLISIITITKTPKSRAATIAFLSSLYAISDQAHKTFTKDILFIQIIR 240
           L+HLL QN+ LID +ISI+ +TKT +SR A IAFLSSLY+ISDQ HK FTKD LFIQ+ R
Sbjct: 181 LEHLLIQNANLIDAVISIVAVTKTSRSRGAAIAFLSSLYSISDQTHKIFTKDNLFIQLSR 240

Query: 241 ALKDQIATKSTLQILLHLTPFGRNRIKAINNGAVFHLVELLLNSS-GRESELAMAVLDRL 300
           AL DQIA K  L+ILLHL PFGRNRIKA+ +G VF LVELLLNS+ GRE ELA+  +D L
Sbjct: 241 ALNDQIAIKPALKILLHLAPFGRNRIKAVKHGVVFDLVELLLNSTVGRECELAIMAMDHL 300

Query: 301 CECAEGRAELLRHGGGMAVVSRKILRVSNLGNEKAVRIVYNVSKNNVGNFGVFEEMVEVG 360
           CECAEGRAELLRHGGGMAVV+RKILRVSN  NEKAVRI+Y+V KNNVGNFGV EEMVEVG
Sbjct: 301 CECAEGRAELLRHGGGMAVVARKILRVSNFSNEKAVRILYHVCKNNVGNFGVAEEMVEVG 360

Query: 361 VVAKLCSMLQVCGSLKTKERIKEILHLLQCVFKGSKCVLVIPSGFDRF 402
            V KLC MLQ+ GSLKT ERIKEILH L+C+FKG+KCVLV+PSGFDRF
Sbjct: 361 AVGKLCLMLQIGGSLKTNERIKEILHFLRCIFKGTKCVLVLPSGFDRF 400

BLAST of Cla97C03G062600 vs. ExPASy TrEMBL
Match: A0A6J1EH89 (RING-type E3 ubiquitin transferase OS=Cucurbita moschata OX=3662 GN=LOC111434282 PE=4 SV=1)

HSP 1 Score: 534.3 bits (1375), Expect = 4.5e-148
Identity = 295/402 (73.38%), Postives = 328/402 (81.59%), Query Frame = 0

Query: 4   IEVPSDFLCPISLQIMRDPVTISTGITYDRESIETWLLCCNNRPVVVLSCPVTKQALSGM 63
           +EVPSDFLCPISLQIMRDPVTISTGITYDRESIE WL    NR VVV SCPVTK  LS +
Sbjct: 3   VEVPSDFLCPISLQIMRDPVTISTGITYDRESIEKWLSSYKNRHVVVPSCPVTKLPLSEL 62

Query: 64  DLTPNHTLRRVIQGWCNLNRRHGVEQIPTPKGEGDRGDVVKLILKEAVRGPRSSRLECLK 123
           DLTPNHTLRR+IQGWC+LN   GVE IPTPKGE DRG VVKL+ KEA+R   SSRLECLK
Sbjct: 63  DLTPNHTLRRLIQGWCSLNAGDGVEPIPTPKGEVDRGQVVKLV-KEAMR-CGSSRLECLK 122

Query: 124 RLKSFVDESERNK----NVESVEFLASILNEDEDEEIIVEEAIEIISNINSPTTLLKHLL 183
           RLK+ V E+ERNK     VES+EFL S+LN   ++E ++EEAIEI+SN NSPTTLLKHLL
Sbjct: 123 RLKAIVVENERNKFYLQEVESMEFLTSVLN--NNDESMIEEAIEILSNTNSPTTLLKHLL 182

Query: 184 TQNSQLIDTLISIITITKTPKSRAATIAFLSSLYAISDQAHKTFTKDILFIQIIRALKDQ 243
           TQN  LI TLISIITITK+  S AA IAFLSSLY ISDQ HKTFT+D LFI + RAL DQ
Sbjct: 183 TQNPNLIQTLISIITITKSSISSAAAIAFLSSLYTISDQTHKTFTQDTLFIHLTRALSDQ 242

Query: 244 IATKSTLQILLHLTPFGRNRIKAINNGAVFHLVELLLNSS-GRESELAMAVLDRLCECAE 303
           IAT  +LQILLHL P GRNR KA+ +GAVFHL++LL  SS  RE ELAMA LDRLCECAE
Sbjct: 243 IATNPSLQILLHLAPHGRNRFKAVKHGAVFHLIQLLFGSSIPRECELAMATLDRLCECAE 302

Query: 304 GRAELLRHGGGMAVVSRKILRVSNLGNEKAVRIVYNVSKNNVGNFGVFEEMVEVGVVAKL 363
           GR ELLRHGGG+AVV+RKILRVSNL NEKAVRI+YNV KNNVGNF VFEEMVEVG V KL
Sbjct: 303 GRVELLRHGGGVAVVARKILRVSNLTNEKAVRILYNVCKNNVGNFRVFEEMVEVGAVGKL 362

Query: 364 CSMLQVCGSLKTKERIKEILHLLQCVFKG-SKCVLVIPSGFD 400
           C++++V GSLK  ER+K ILHL+Q  F G SKCV+VIP  FD
Sbjct: 363 CAVIEVGGSLKIIERVKVILHLIQRAFDGSSKCVIVIPPRFD 400

BLAST of Cla97C03G062600 vs. TAIR 10
Match: AT2G35930.1 (plant U-box 23 )

HSP 1 Score: 288.1 bits (736), Expect = 1.1e-77
Identity = 170/402 (42.29%), Postives = 256/402 (63.68%), Query Frame = 0

Query: 3   EIEVPSDFLCPISLQIMRDPVTISTGITYDRESIETWLLCCNNRPVVVLSCPVTKQALSG 62
           EIE+P  FLCPISL+IM+DPV +STGITYDR+SIE WL           SCPVTKQ ++ 
Sbjct: 9   EIEIPPFFLCPISLEIMKDPVIVSTGITYDRDSIEKWLFAGKKN-----SCPVTKQDITD 68

Query: 63  MDLTPNHTLRRVIQGWCNLNRRHGVEQIPTPKGEGDRGDVVKLILKEAVRGPRSSRLECL 122
            DLTPNHTLRR+IQ WC LN  +GVE+IPTP+    + ++ KLI   A      ++++CL
Sbjct: 69  ADLTPNHTLRRLIQSWCTLNASYGVERIPTPRPPICKSEIEKLIRDSA--SSHENQVKCL 128

Query: 123 KRLKSFVDESERNKN-VESV---EFLASILNEDEDEEIIVEEAIEIISNINSPTTLLKHL 182
           KRL+  V E+  NK  +E+    EFLA+I++ D +   + +EA+ ++ ++ +  T+LK+L
Sbjct: 129 KRLRQIVSENATNKRCLEAAGVPEFLANIVSNDSENGSLTDEALNLLYHLETSETVLKNL 188

Query: 183 LT--QNSQLIDTLISIITITKTPKSRAATIAFLSSLYAISDQAHKTFTKDILFIQIIRAL 242
           L   +++ ++ +L  I+      +SR      L ++  ++D       K  +F ++++ L
Sbjct: 189 LNNKKDNNIVKSLTKIMQ-RGMYESRVYATLLLKNILEVADPMQSMTLKPEVFTEVVQIL 248

Query: 243 KDQI---ATKSTLQILLHLTPFGRNRIKAINNGAVFHLVELLLN----SSGRESELAMAV 302
            D+I   ATK+ + IL+++ P+GRNR KA+  G +  ++ELL++    S  R  E+AM V
Sbjct: 249 DDRISQKATKAAMHILVNICPWGRNRHKAVEAGVISVIIELLMDESFTSERRGPEMAMVV 308

Query: 303 LDRLCECAEGRAELLRHGGGMAVVSRKILRVSNLGNEKAVRIVYNVSKNNVGNFGVFEEM 362
           LD LC+CAEGRAE L HG  +AVV +KILRVS   +++AVR++ +V +       +  EM
Sbjct: 309 LDLLCQCAEGRAEFLNHGAAIAVVCKKILRVSQTASDRAVRVLLSVGR-FCATPALLHEM 368

Query: 363 VEVGVVAKLCSMLQVCGSLKTKERIKEILHLLQCVFKGSKCV 392
           +++GVVAKLC +LQV    KTKE+ KE+L L   V+K S C+
Sbjct: 369 LQLGVVAKLCLVLQVSCGGKTKEKAKELLKLHARVWKDSPCL 401

BLAST of Cla97C03G062600 vs. TAIR 10
Match: AT3G52450.1 (plant U-box 22 )

HSP 1 Score: 286.6 bits (732), Expect = 3.1e-77
Identity = 181/431 (42.00%), Postives = 256/431 (59.40%), Query Frame = 0

Query: 3   EIEVPSDFLCPISLQIMRDPVTISTGITYDRESIETWLLCCNNRPVVVLSCPVTKQALSG 62
           EIE+PS FLCPISL IM+DPV +STGITYDRESIE WL           SCPVTKQ ++ 
Sbjct: 4   EIEIPSFFLCPISLDIMKDPVIVSTGITYDRESIEKWLFSGKKN-----SCPVTKQVITE 63

Query: 63  MDLTPNHTLRRVIQGWCNLNRRHGVEQIPTPKGEGDRGDVVKLILKEAVRGPRSSRLECL 122
            DLTPNHTLRR+IQ WC LN  +G+E+IPTPK    + ++ KLI + +      ++++CL
Sbjct: 64  TDLTPNHTLRRLIQSWCTLNASYGIERIPTPKPPICKSEIEKLIKESS--SSHLNQVKCL 123

Query: 123 KRLKSFVDESERNKN----VESVEFLASIL-----------------------------N 182
           KRL+  V E+  NK      E  EFLA+I+                             N
Sbjct: 124 KRLRQIVSENTTNKRCLEAAEVPEFLANIVSNSVDTYNSPSSSLSSSNLNDMCQSNMLEN 183

Query: 183 EDEDEEIIVEEAIEIISNINSPTTLLKHLLT--QNSQLIDTLISIITITKTPKSRAATIA 242
             +    +++EA+ ++ ++++  T LK LL   + + L+ TL  I+      +SRA    
Sbjct: 184 RFDSSRSLMDEALSVLYHLDTSETALKSLLNNKKGTNLVKTLTKIMQ-RGIYESRAYAAL 243

Query: 243 FLSSLYAISDQAHKTFTKDILFIQIIRALKDQI---ATKSTLQILLHLTPFGRNRIKAIN 302
            L  L  ++D       +  LF ++I+ L DQI   AT+S +QIL+   P+GRNR KA+ 
Sbjct: 244 LLKKLLEVADPMQIILLERELFGEVIQILHDQISHKATRSAMQILVITCPWGRNRHKAVE 303

Query: 303 NGAVFHLVELLL----NSSGRESELAMAVLDRLCECAEGRAELLRHGGGMAVVSRKILRV 362
            G +  ++ELL+    +S  R SE+AM VLD LC+CAEGRAE L HG  +AVVS+KILRV
Sbjct: 304 GGTISMIIELLMDDTFSSERRNSEMAMVVLDMLCQCAEGRAEFLNHGAAIAVVSKKILRV 363

Query: 363 SNLGNEKAVRIVYNVSKNNVGNFGVFEEMVEVGVVAKLCSMLQVCGSLKTKERIKEILHL 392
           S + +E+AVR++ +V +       + +EM+++GVVAKLC +LQV    KTKE+ KE+L L
Sbjct: 364 SQITSERAVRVLLSVGR-FCATPSLLQEMLQLGVVAKLCLVLQVSCGNKTKEKAKELLKL 423

BLAST of Cla97C03G062600 vs. TAIR 10
Match: AT3G11840.1 (plant U-box 24 )

HSP 1 Score: 194.1 bits (492), Expect = 2.1e-49
Identity = 154/428 (35.98%), Postives = 225/428 (52.57%), Query Frame = 0

Query: 3   EIEVPSDFLCPISLQIMRDPVTISTGITYDRESIETWLLCCNNRPVVVLSCPVTKQALS- 62
           EIE+P+ F+CPISL+IM+DPVT  +GITYDR++I  WL         V SCPVTKQ L  
Sbjct: 21  EIEIPNYFICPISLEIMKDPVTTVSGITYDRQNIVKWL-------EKVPSCPVTKQPLPL 80

Query: 63  GMDLTPNHTLRRVIQGWCNLNRRHGVEQIPTPKGEGDRGDVVKLILKEAVRGPRS-SRLE 122
             DLTPNH LRR+IQ WC  N   GV +I TP+    + +VV+ I      G  +  R E
Sbjct: 81  DSDLTPNHMLRRLIQHWCVENETRGVVRISTPRVPPGKLNVVEEIKNLKKFGQEALGREE 140

Query: 123 CLKRLKSF-VDESERNKNVES------VEFLASILNEDEDEEIIV---EEAIEIISNINS 182
            L++L+   +D + R    E       + F+    +EDED    +   +E++ ++  I  
Sbjct: 141 TLQKLEVLAMDGNNRRLMCECGVHKSLILFVVKCTSEDEDGRRRIKGLDESLRLLHLIGI 200

Query: 183 PTTLLKHLLTQNSQLIDTLISIITITKTPKSRAATIAFLSSLYAISDQAHKTFTKDILFI 242
           P+   K +L +N +++++L  ++   +   S+A TI  L +L   +           +F 
Sbjct: 201 PSNDAKTILMENDRVMESLTWVLH-QEDFLSKAYTIVLLRNLTEYTSSHIVERLNPEIFK 260

Query: 243 QIIRALKDQI--------------------------------------ATKSTLQILLHL 302
            II  LKD +                                      A  + L ILL  
Sbjct: 261 GIIGFLKDVVNSVNRTSPTVRETVQSSSRPSLGKTEPSKLDHSLVIKQAVTAALMILLET 320

Query: 303 TPFGRNRIKAINNGAVFHLVELLLNSSG--RESELAMAVLDRLCECAEGRAELLRHGGGM 362
           + + RNR   ++ GAV  L+EL ++ +G  R +EL + VL RLC CA GRAE+L H GG+
Sbjct: 321 SSWSRNRSLLVDLGAVSELIELEISYTGEKRITELMLGVLSRLCCCANGRAEILAHRGGI 380

Query: 363 AVVSRKILRVSNLGNEKAVRIVYNVSKNNVGNFGVFEEMVEVGVVAKLCSMLQVCGSLKT 379
           AVV++++LRVS   +++A+ I+  VSK +  N  V EEMV VG V KLCS+L +   L  
Sbjct: 381 AVVTKRLLRVSPAADDRAISILTTVSKFSPENM-VVEEMVNVGTVEKLCSVLGMDCGLNL 439

BLAST of Cla97C03G062600 vs. TAIR 10
Match: AT3G19380.1 (plant U-box 25 )

HSP 1 Score: 149.1 bits (375), Expect = 7.8e-36
Identity = 126/396 (31.82%), Postives = 204/396 (51.52%), Query Frame = 0

Query: 4   IEVPSDFLCPISLQIMRDPVTISTGITYDRESIETWLLCCNNRPVVVLSCPVTKQALSGM 63
           I++P  F CPISL++M+DPVT+ TG TYDR SIE+W+   NN      +CPVT+  LS  
Sbjct: 12  IQIPYHFRCPISLELMQDPVTVCTGQTYDRASIESWVSIGNN-----TTCPVTRAPLSDF 71

Query: 64  DLTPNHTLRRVIQGWCNLNRRHGVEQIPTPKGEGDRGDVVKLILK-EAVRGPR---SSRL 123
            L PNHTLRR+IQ WC  NR +GVE+IPTPK   D   V  L+ +  A+ G      SR 
Sbjct: 72  TLIPNHTLRRLIQEWCVANRSNGVERIPTPKQPADPTSVRALLSQASAITGTHVSVRSRA 131

Query: 124 ECLKRLKSFVDESERNKNV----ESVEFLASIL-NEDEDEEIIVEE-AIEIISNINSPTT 183
             L+RL+ F  +S++N+ +     + E L  IL +E    E++ E  A+ ++  I  P  
Sbjct: 132 AALRRLRGFARDSDKNRVLIAAHNATEILIKILFSETTSSELVSESLALLVMLPITEPNQ 191

Query: 184 LLKHLLTQNSQLIDTLISII--TITKTPKSRAATIAFLSSLYAISDQAHKTFTKDILFIQ 243
            +   ++ +   ++ L  ++  +  +T  + AA I  +S+    +D        + +F  
Sbjct: 192 FVS--ISSDPGRVEFLTRLLFDSSIETRVNAAALIEIVSTGTKSADLKGSISNSESVFEG 251

Query: 244 IIRALKDQIATKSTLQI----LLHLTPFGRNRIKAINNGAVFHLVELLLNSSGR-ESELA 303
           ++  L++ I+++  L+I    L  L      R  AI  GA   L++ L     R ++E A
Sbjct: 252 VLDLLRNPISSRRALKIGIKTLFALCSVKSTRHIAITAGAPEILIDRLAADFDRCDTERA 311

Query: 304 MAVLDRLCECAEGRAELLRHGGGMAVVSRKILRVSNLGNEKAVRIVYNVSKNNVGNFGVF 363
           +A ++ LC   EG A    H   + ++ + ILRVS+   E A   +  +           
Sbjct: 312 LATVELLCRTPEGCAAFGEHALTVPLLVKTILRVSDRATEYAAGALLALC---TAEERWR 371

Query: 364 EEMVEVGVVAKLCSMLQVCGSLKTKERIKEILHLLQ 383
           EE    GVV +L  M+Q   + + K++ +++L LL+
Sbjct: 372 EEAAGAGVVVQLLLMVQSECTERAKKKAQKLLKLLR 397

BLAST of Cla97C03G062600 vs. TAIR 10
Match: AT1G66160.1 (CYS, MET, PRO, and GLY protein 1 )

HSP 1 Score: 138.3 bits (347), Expect = 1.4e-32
Identity = 113/409 (27.63%), Postives = 199/409 (48.66%), Query Frame = 0

Query: 3   EIEVPSDFLCPISLQIMRDPVTISTGITYDRESIETWLLCCNNRPVVVLSCPVTKQALSG 62
           EI +PS F CPIS ++M+DPV I++GITYDRE+IE W            +CPVT   L+ 
Sbjct: 30  EITIPSQFQCPISYELMKDPVIIASGITYDRENIEKWF------ESGYQTCPVTNTVLTS 89

Query: 63  MDLTPNHTLRRVIQGWCNLNRRHGVEQIPTPK---GEGDRGDVVKLILKEAVRGPRSSRL 122
           ++  PNHT+RR+IQGWC  +   G+E+IPTP+         ++ + +     RG  ++ +
Sbjct: 90  LEQIPNHTIRRMIQGWCGSSLGGGIERIPTPRVPVTSHQVSEICERLSAATRRGDYAACM 149

Query: 123 ECLKRLKSFVDESERNKNVESVEFLASIL-------NEDEDEEIIVEEAIEIISNINSPT 182
           E + ++     ESERN+          +L       +E+ +  +++EE + ++      T
Sbjct: 150 EMVTKMTRLGKESERNRKCVKENGAGLVLCVCFDAFSENANASLLLEETVSVL------T 209

Query: 183 TLLKHLLTQNSQLIDT-----LISII---------TITKTPKSRAATIAFLSSLYAISDQ 242
            +L   L   S+L  T     L+ ++          I +  +     +  L+ +  + + 
Sbjct: 210 WMLPIGLEGQSKLTTTSSFNRLVELLRNGDQNAAFLIKELLELNVTHVHALTKINGVQEA 269

Query: 243 AHKTFTKDILFIQIIRALKDQIAT-KSTLQILLHLTPFGRNRIKAINNGAVFHLVELLLN 302
             K+  +D   +  + ++   I T + T+   L L               V   VE+L++
Sbjct: 270 FMKSINRDSTCVNSLISIHHMILTNQETVSRFLEL-------------DLVNITVEMLVD 329

Query: 303 SSGRESELAMAVLDRLCECAEGRAELLRHGGGMAVVSRKILRVSNLGNEKAVRIVYNVSK 362
           S     E A+ VL+ +CE  EGR ++ R+   + ++ +KIL++S    +  V +++ V K
Sbjct: 330 SENSVCEKALTVLNVICETKEGREKVRRNKLVIPILVKKILKISE--KKDLVSVMWKVCK 389

Query: 363 NNVGNFGVFEEMVEVGVVAKLCSMLQVCGSLKTKERIKEILHLLQCVFK 387
           +  G+    EE + +G   KL  MLQV     TKE++ E+L ++  V K
Sbjct: 390 S--GDGSEVEEALRLGAFKKLVVMLQVGCGEGTKEKVTELLKMMNKVMK 409

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
XP_038879749.19.8e-18284.73E3 ubiquitin-protein ligase PUB23-like isoform X1 [Benincasa hispida][more]
XP_038879750.13.7e-17384.44E3 ubiquitin-protein ligase PUB23-like isoform X2 [Benincasa hispida][more]
XP_004139144.26.6e-17079.27E3 ubiquitin-protein ligase PUB23 [Cucumis sativus] >KGN66593.1 hypothetical pro... [more]
XP_008450285.15.6e-16979.41PREDICTED: E3 ubiquitin-protein ligase PUB22-like [Cucumis melo][more]
XP_022961129.18.9e-15974.26E3 ubiquitin-protein ligase PUB23-like [Cucurbita moschata][more]
Match NameE-valueIdentityDescription
Q84TG31.5e-7642.29E3 ubiquitin-protein ligase PUB23 OS=Arabidopsis thaliana OX=3702 GN=PUB23 PE=1 ... [more]
Q9SVC64.4e-7642.00E3 ubiquitin-protein ligase PUB22 OS=Arabidopsis thaliana OX=3702 GN=PUB22 PE=1 ... [more]
Q9SF153.0e-4835.98E3 ubiquitin-protein ligase PUB24 OS=Arabidopsis thaliana OX=3702 GN=PUB24 PE=1 ... [more]
Q9LT791.1e-3431.82U-box domain-containing protein 25 OS=Arabidopsis thaliana OX=3702 GN=PUB25 PE=2... [more]
Q9C8D11.9e-3127.63U-box domain-containing protein 20 OS=Arabidopsis thaliana OX=3702 GN=PUB20 PE=1... [more]
Match NameE-valueIdentityDescription
A0A0A0LXW13.2e-17079.27RING-type E3 ubiquitin transferase OS=Cucumis sativus OX=3659 GN=Csa_1G635930 PE... [more]
A0A1S3BNV92.7e-16979.41RING-type E3 ubiquitin transferase OS=Cucumis melo OX=3656 GN=LOC103491935 PE=4 ... [more]
A0A6J1HD284.3e-15974.26RING-type E3 ubiquitin transferase OS=Cucurbita moschata OX=3662 GN=LOC111461732... [more]
A0A6J1JH481.2e-15673.53RING-type E3 ubiquitin transferase OS=Cucurbita maxima OX=3661 GN=LOC111485066 P... [more]
A0A6J1EH894.5e-14873.38RING-type E3 ubiquitin transferase OS=Cucurbita moschata OX=3662 GN=LOC111434282... [more]
Match NameE-valueIdentityDescription
AT2G35930.11.1e-7742.29plant U-box 23 [more]
AT3G52450.13.1e-7742.00plant U-box 22 [more]
AT3G11840.12.1e-4935.98plant U-box 24 [more]
AT3G19380.17.8e-3631.82plant U-box 25 [more]
AT1G66160.11.4e-3227.63CYS, MET, PRO, and GLY protein 1 [more]
InterPro
Analysis Name: InterPro Annotations of Watermelon (97103) v2.5
Date Performed: 2022-01-31
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR003613U box domainSMARTSM00504Ubox_2coord: 9..78
e-value: 3.6E-26
score: 102.9
IPR003613U box domainPFAMPF04564U-boxcoord: 6..82
e-value: 1.8E-17
score: 63.2
IPR003613U box domainPROSITEPS51698U_BOXcoord: 5..85
score: 31.297853
IPR013083Zinc finger, RING/FYVE/PHD-typeGENE3D3.30.40.10Zinc/RING finger domain, C3HC4 (zinc finger)coord: 1..82
e-value: 2.3E-26
score: 93.4
IPR011989Armadillo-like helicalGENE3D1.25.10.10coord: 104..390
e-value: 4.3E-12
score: 47.3
NoneNo IPR availablePANTHERPTHR22849:SF11OS11G0573500 PROTEINcoord: 2..392
NoneNo IPR availablePANTHERPTHR22849WDSAM1 PROTEINcoord: 2..392
NoneNo IPR availableCDDcd16664RING-Ubox_PUBcoord: 10..58
e-value: 7.72413E-21
score: 83.0519
NoneNo IPR availableSUPERFAMILY57850RING/U-boxcoord: 3..87
IPR016024Armadillo-type foldSUPERFAMILY48371ARM repeatcoord: 137..366

Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
Cla97C03G062600.1Cla97C03G062600.1mRNA


GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
biological_process GO:0016567 protein ubiquitination
molecular_function GO:0004842 ubiquitin-protein transferase activity