Cla97C03G060842 (gene) Watermelon (97103) v2.5

Overview


Name	Cla97C03G060842
Type	gene
Organism	Citrullus lanatus subsp. vulgaris cv. 97103 (Watermelon (97103) v2.5)
Description	tRNA pseudouridine synthase B
Location	Cla97Chr03: 14877458 .. 14879216 (-)
RNA-Seq Expression	Cla97C03G060842
Synteny	Cla97C03G060842

Sequences

The following sequences are available for this feature:

Gene sequence (with intron)

Legend: polypeptideexonCDS

Hold the cursor over a type above to highlight its positions in the sequence below.

ATGAATGACAAAGGGTTGGTTTTCTTTGATGAGGGGAAACCAGAGAAGGATAATAAGGGGTCGGTGAAAGATGTGGCAGAGAAGAAGGTGGAGGAGTTCTTCAAGTGTTTGAAGAAAGTTCCAGCTAAAGATTCTGAAATTGGTCAGATGGATCCATATCTTTTAACGAGGCATACGGAGCTCCCTGCCAAATGGGATAGTCCTTCTGGGACAGTGGTTTTGCTGAACAAACCTAAAGGGTGGACTTCATTTACGGTATGTGGAAAACTTTGGCGCCTAGTCAAAGTGAAAAAGGTAGGTCATGCAGGTACTCTTGATCCTATGGCTACTGGTTTGTTGATAGTGTGTGTTGGTAAAGCGATGAAGTTGGTAGACAGATATCAAGGTATGAGCTACAGTGGAGTTTTCCGTTTAGGAGAGGCTACTTCAACTTGGGATGCTGATTCACCGGTAATTCAGCGAGAGCCTTGGGAGCACATCAAAGATGATGACATCAAAAAAGCTGCTGCCTCTTTTTGTGGGGAAATTTGGCAAGTACCTCCCATGTTTTCTGCCATTAAAGTTGGAGGTGAGAAGATGTACGAGAAAGCAAGAAGGGGAGAAAGTATTGAGCTCTCCCCAAGACGGATATCAATTTTCAAATTTGATATTGAACGTAGTTTAGATGACAGGCAAAGCTTGATTTTTCGAGTAACATGCTCTAAAGGAACATACATACGGTCACCTTGTGCAGATCTTGGGAAGGTTCTTGGCAACTATTTCAAAAAAAATACCACCTATTGCAATTACCCTTTCTTAAATTCTTGCTTTGAGCAAGGAAAAAATTTAGGCCATGAATATATTAGGGGCTTGAGAAAGTTGGCGTTGTATTTTATACACTTGACCTCAGCACCTGAATTATTCATCTAGTTCTTCACATCTCACTCATTTATTCATCTAGTTCTCCACATCTCACTTCATCCAACTTGAGGCTGTCTGGTGCTTGTATCACCCTTATCCTGCGAACTAATTTGTGATTTGAAATTATCGATTGAACTTGCTACCGTTTTTGTGAGTGTGTGCAATGTTTCAAAAGGTTTTTGAGGCATAGTCCATGATGCTCACCTGAGGTTGAGGCTCGAACTTTTAGCCTCAAAGACTTGCACTTTCTTTAGATCATAAGTGGCTGTGGCTTCTTTAGGCTTCTTATAGAGAGGCCTGTGTTCATGAGAATGCATCCAGGTTTGCAGCCTTTACCTCATGAAATAGCCAGAGGAATATATTTAACTTGTTAACTAAAATTATATTAAGAAAATTTGCTTAGACTTTAATGTTTTAATATACCATGAGTTTCGGTAACTTAGGCCCTTACTAGTTTTACCATCAAGGTAAAAAAGAACTTTGAAGCAAAGTGAAGGATGAGTTGAGTCTATTATGATATTTATTTAAGTGACATTTGACATGCAGGAGTTTCTTTTCCTCCTTTTTTGTACTTCTTGATAATCAATTTAATTTAACAATACTTGATCTTGGTAGGAGTGGGAGAGGGAATGATATCTAATATTGATAGGGTTTGGGTTAGTTACTTTACCTCTATATTACTTGCTGACTTAGCTTAAAAAATGAAATTATTGATAAATGAAATTAGGGGGAAATGTTAGTTCTTTCCAGTTAAGAGGCCATTTCTGATGCCATTGCATTTCATGCTTGTTAACATCAGCTGATGGTGATGAGTATGAGTATATTTAGAGGATCCTATATTCATAATCTGATAATCTAA

mRNA sequence

ATGAATGACAAAGGGTTGGTTTTCTTTGATGAGGGGAAACCAGAGAAGGATAATAAGGGGTCGGTGAAAGATGTGGCAGAGAAGAAGGTGGAGGAGTTCTTCAAGTGTTTGAAGAAAGTTCCAGCTAAAGATTCTGAAATTGGTCAGATGGATCCATATCTTTTAACGAGGCATACGGAGCTCCCTGCCAAATGGGATAGTCCTTCTGGGACAGTGGTTTTGCTGAACAAACCTAAAGGGTGGACTTCATTTACGGTATGTGGAAAACTTTGGCGCCTAGTCAAAGTGAAAAAGGTAGGTCATGCAGGTACTCTTGATCCTATGGCTACTGGTTTGTTGATAGTGTGTGTTGGTAAAGCGATGAAGTTGGTAGACAGATATCAAGGTATGAGCTACAGTGGAGTTTTCCGTTTAGGAGAGGCTACTTCAACTTGGGATGCTGATTCACCGGTAATTCAGCGAGAGCCTTGGGAGCACATCAAAGATGATGACATCAAAAAAGCTGCTGCCTCTTTTTGTGGGGAAATTTGGCAAGTACCTCCCATGTTTTCTGCCATTAAAGTTGGAGGAGTGGGAGAGGGAATGATATCTAATATTGATAGGGTTTGGCTGATGGTGATGAGTATGAGTATATTTAGAGGATCCTATATTCATAATCTGATAATCTAA

Coding sequence (CDS)

ATGAATGACAAAGGGTTGGTTTTCTTTGATGAGGGGAAACCAGAGAAGGATAATAAGGGGTCGGTGAAAGATGTGGCAGAGAAGAAGGTGGAGGAGTTCTTCAAGTGTTTGAAGAAAGTTCCAGCTAAAGATTCTGAAATTGGTCAGATGGATCCATATCTTTTAACGAGGCATACGGAGCTCCCTGCCAAATGGGATAGTCCTTCTGGGACAGTGGTTTTGCTGAACAAACCTAAAGGGTGGACTTCATTTACGGTATGTGGAAAACTTTGGCGCCTAGTCAAAGTGAAAAAGGTAGGTCATGCAGGTACTCTTGATCCTATGGCTACTGGTTTGTTGATAGTGTGTGTTGGTAAAGCGATGAAGTTGGTAGACAGATATCAAGGTATGAGCTACAGTGGAGTTTTCCGTTTAGGAGAGGCTACTTCAACTTGGGATGCTGATTCACCGGTAATTCAGCGAGAGCCTTGGGAGCACATCAAAGATGATGACATCAAAAAAGCTGCTGCCTCTTTTTGTGGGGAAATTTGGCAAGTACCTCCCATGTTTTCTGCCATTAAAGTTGGAGGAGTGGGAGAGGGAATGATATCTAATATTGATAGGGTTTGGCTGATGGTGATGAGTATGAGTATATTTAGAGGATCCTATATTCATAATCTGATAATCTAA

Protein sequence

MNDKGLVFFDEGKPEKDNKGSVKDVAEKKVEEFFKCLKKVPAKDSEIGQMDPYLLTRHTELPAKWDSPSGTVVLLNKPKGWTSFTVCGKLWRLVKVKKVGHAGTLDPMATGLLIVCVGKAMKLVDRYQGMSYSGVFRLGEATSTWDADSPVIQREPWEHIKDDDIKKAAASFCGEIWQVPPMFSAIKVGGVGEGMISNIDRVWLMVMSMSIFRGSYIHNLII

Homology

[Close]

BLAST of Cla97C03G060842 vs. NCBI nr
Match: XP_038891059.1 (uncharacterized protein LOC120080471 [Benincasa hispida])

HSP 1 Score: 366.7 bits (940), Expect = 1.4e-97
Identity = 178/192 (92.71%), Postives = 182/192 (94.79%), Query Frame = 0


Query: 1   MNDKGLVFFDEGKPEKDNKGSVKDVAEKKVEEFFKCLKKVPAKDSEIGQMDPYLLTRHTE 60
           M+DKGLVFFDEGKPE +NKGSVKDV EKKVEEFFKCLKKVPAKDSEIG  +PYLLTRHTE
Sbjct: 257 MDDKGLVFFDEGKPENENKGSVKDVTEKKVEEFFKCLKKVPAKDSEIGPTEPYLLTRHTE 316

Query: 61  LPAKWDSPSGTVVLLNKPKGWTSFTVCGKLWRLVKVKKVGHAGTLDPMATGLLIVCVGKA 120
           LPAKWDSP GTVVLLNKPKGWTSFTVCGKL RLVKVKKVGHAGTLDPMATGLLIVCVGKA
Sbjct: 317 LPAKWDSPCGTVVLLNKPKGWTSFTVCGKLRRLVKVKKVGHAGTLDPMATGLLIVCVGKA 376

Query: 121 MKLVDRYQGM--SYSGVFRLGEATSTWDADSPVIQREPWEHIKDDDIKKAAASFCGEIWQ 180
            KLV+RYQGM  SYSGVFRLGEATSTWDADSPVIQREPWEHIKD DIKKAAASFCGEIWQ
Sbjct: 377 TKLVERYQGMIKSYSGVFRLGEATSTWDADSPVIQREPWEHIKDGDIKKAAASFCGEIWQ 436

Query: 181 VPPMFSAIKVGG 191
           VPPMFSAIKVGG
Sbjct: 437 VPPMFSAIKVGG 448

[Close]

BLAST of Cla97C03G060842 vs. NCBI nr
Match: XP_022993331.1 (uncharacterized protein LOC111489372 [Cucurbita maxima] >XP_022993332.1 uncharacterized protein LOC111489372 [Cucurbita maxima])

HSP 1 Score: 364.0 bits (933), Expect = 9.2e-97
Identity = 176/192 (91.67%), Postives = 182/192 (94.79%), Query Frame = 0


Query: 1   MNDKGLVFFDEGKPEKDNKGSVKDVAEKKVEEFFKCLKKVPAKDSEIGQMDPYLLTRHTE 60
           +++KGLVFFD G+PEKDNKGSVKDV EKKVEEFFKCLKKVPAKDSEIGQ +PYLLTRHTE
Sbjct: 255 VDEKGLVFFDGGRPEKDNKGSVKDVTEKKVEEFFKCLKKVPAKDSEIGQTEPYLLTRHTE 314

Query: 61  LPAKWDSPSGTVVLLNKPKGWTSFTVCGKLWRLVKVKKVGHAGTLDPMATGLLIVCVGKA 120
           LPAKWDSP GTVVL+NKPKGWTSFTVCGKL RLVKVKKVGHAGTLDPMATGLLIVCVGKA
Sbjct: 315 LPAKWDSPCGTVVLVNKPKGWTSFTVCGKLRRLVKVKKVGHAGTLDPMATGLLIVCVGKA 374

Query: 121 MKLVDRYQGM--SYSGVFRLGEATSTWDADSPVIQREPWEHIKDDDIKKAAASFCGEIWQ 180
            KL DRYQGM  SYSGVFRLGEATSTWDADS VIQREPWEHIKDDDIKKAAASFCGEIWQ
Sbjct: 375 TKLADRYQGMIKSYSGVFRLGEATSTWDADSAVIQREPWEHIKDDDIKKAAASFCGEIWQ 434

Query: 181 VPPMFSAIKVGG 191
           VPPMFSAIKVGG
Sbjct: 435 VPPMFSAIKVGG 446

[Close]

BLAST of Cla97C03G060842 vs. NCBI nr
Match: XP_022938741.1 (uncharacterized protein LOC111444875 [Cucurbita moschata] >KAG7016215.1 truB [Cucurbita argyrosperma subsp. argyrosperma])

HSP 1 Score: 363.6 bits (932), Expect = 1.2e-96
Identity = 176/192 (91.67%), Postives = 181/192 (94.27%), Query Frame = 0


Query: 1   MNDKGLVFFDEGKPEKDNKGSVKDVAEKKVEEFFKCLKKVPAKDSEIGQMDPYLLTRHTE 60
           +++KGLVFFD G+PEKDNKGSVKDV EKKVEEFFKCLKKVPAKDSEIGQM PYLLTRHTE
Sbjct: 255 VDEKGLVFFDGGRPEKDNKGSVKDVTEKKVEEFFKCLKKVPAKDSEIGQMGPYLLTRHTE 314

Query: 61  LPAKWDSPSGTVVLLNKPKGWTSFTVCGKLWRLVKVKKVGHAGTLDPMATGLLIVCVGKA 120
           LPAKWDSP GTVVL+NKPKGWTSFTVCGKL RLVKVKKVGHAGTLDPMATGLLIVCVGKA
Sbjct: 315 LPAKWDSPCGTVVLVNKPKGWTSFTVCGKLRRLVKVKKVGHAGTLDPMATGLLIVCVGKA 374

Query: 121 MKLVDRYQGM--SYSGVFRLGEATSTWDADSPVIQREPWEHIKDDDIKKAAASFCGEIWQ 180
            KL DRYQGM  SYSGVFRLGEATSTWDADS VIQREPWEHIKDDDIKKA ASFCGEIWQ
Sbjct: 375 TKLADRYQGMIKSYSGVFRLGEATSTWDADSAVIQREPWEHIKDDDIKKAVASFCGEIWQ 434

Query: 181 VPPMFSAIKVGG 191
           VPPMFSAIKVGG
Sbjct: 435 VPPMFSAIKVGG 446

[Close]

BLAST of Cla97C03G060842 vs. NCBI nr
Match: KAG6578676.1 (hypothetical protein SDJN03_23124, partial [Cucurbita argyrosperma subsp. sororia])

HSP 1 Score: 362.5 bits (929), Expect = 2.7e-96
Identity = 175/192 (91.15%), Postives = 181/192 (94.27%), Query Frame = 0


Query: 1   MNDKGLVFFDEGKPEKDNKGSVKDVAEKKVEEFFKCLKKVPAKDSEIGQMDPYLLTRHTE 60
           +++KGLVFFD G+P+KDNKGSVKDV EKKVEEFFKCLKKVPAKDSEIGQM PYLLTRHTE
Sbjct: 255 VDEKGLVFFDGGRPDKDNKGSVKDVTEKKVEEFFKCLKKVPAKDSEIGQMGPYLLTRHTE 314

Query: 61  LPAKWDSPSGTVVLLNKPKGWTSFTVCGKLWRLVKVKKVGHAGTLDPMATGLLIVCVGKA 120
           LPAKWDSP GTVVL+NKPKGWTSFTVCGKL RLVKVKKVGHAGTLDPMATGLLIVCVGKA
Sbjct: 315 LPAKWDSPCGTVVLVNKPKGWTSFTVCGKLRRLVKVKKVGHAGTLDPMATGLLIVCVGKA 374

Query: 121 MKLVDRYQGM--SYSGVFRLGEATSTWDADSPVIQREPWEHIKDDDIKKAAASFCGEIWQ 180
            KL DRYQGM  SYSGVFRLGEATSTWDADS VIQREPWEHIKDDDIKKA ASFCGEIWQ
Sbjct: 375 TKLADRYQGMIKSYSGVFRLGEATSTWDADSAVIQREPWEHIKDDDIKKAVASFCGEIWQ 434

Query: 181 VPPMFSAIKVGG 191
           VPPMFSAIKVGG
Sbjct: 435 VPPMFSAIKVGG 446

[Close]

BLAST of Cla97C03G060842 vs. NCBI nr
Match: KAA0037597.1 (TruB_N domain-containing protein [Cucumis melo var. makuwa] >TYK09336.1 TruB_N domain-containing protein [Cucumis melo var. makuwa])

HSP 1 Score: 361.3 bits (926), Expect = 6.0e-96
Identity = 174/192 (90.62%), Postives = 181/192 (94.27%), Query Frame = 0


Query: 1   MNDKGLVFFDEGKPEKDNKGSVKDVAEKKVEEFFKCLKKVPAKDSEIGQMDPYLLTRHTE 60
           M+DKG+VFFDEGKPEK+NKGSV DV EKKVEEFFKCLKK PAKDS+IGQ +PYLLTRH E
Sbjct: 256 MDDKGMVFFDEGKPEKENKGSVTDVTEKKVEEFFKCLKKGPAKDSKIGQAEPYLLTRHME 315

Query: 61  LPAKWDSPSGTVVLLNKPKGWTSFTVCGKLWRLVKVKKVGHAGTLDPMATGLLIVCVGKA 120
           LPA+WDSP GTVVLLNKPKGWTSFTVCGKL RLVKV+KVGHAGTLDPMATGLLIVCVGKA
Sbjct: 316 LPARWDSPCGTVVLLNKPKGWTSFTVCGKLRRLVKVQKVGHAGTLDPMATGLLIVCVGKA 375

Query: 121 MKLVDRYQGM--SYSGVFRLGEATSTWDADSPVIQREPWEHIKDDDIKKAAASFCGEIWQ 180
            KLVDRYQGM   YSGVFRLGEATSTWDADSPVIQREPWEHIKDDDIKKAAASFCGEIWQ
Sbjct: 376 TKLVDRYQGMIKGYSGVFRLGEATSTWDADSPVIQREPWEHIKDDDIKKAAASFCGEIWQ 435

Query: 181 VPPMFSAIKVGG 191
           VPPMFSAIKVGG
Sbjct: 436 VPPMFSAIKVGG 447

[Close]

BLAST of Cla97C03G060842 vs. ExPASy Swiss-Prot
Match: Q5LJ29 (tRNA pseudouridine synthase B OS=Bacteroides fragilis (strain ATCC 25285 / DSM 2151 / JCM 11019 / NCTC 9343) OX=272559 GN=truB PE=3 SV=1)

HSP 1 Score: 113.2 bits (282), Expect = 3.7e-24
Identity = 60/129 (46.51%), Postives = 79/129 (61.24%), Query Frame = 0


Query: 70  GTVVLLNKPKGWTSFTVCG----KLWRLVKVK--KVGHAGTLDPMATGLLIVCVGKAMKL 129
           G V+  NKP GWTSF V G     + R +KVK  KVGHAGTLDP+ATG++IVC GKA K 
Sbjct: 6   GEVLYFNKPLGWTSFKVVGHARYHMCRRMKVKKLKVGHAGTLDPLATGVMIVCTGKATKR 65

Query: 130 VD--RYQGMSYSGVFRLGEATSTWDADSPVIQREPWEHIKDDDIKKAAASFCGEIWQVPP 189
           ++  +Y    Y    +LG  T ++D +  +    P EHI  + ++K   +F GEI Q+PP
Sbjct: 66  IEEFQYHTKEYVATIQLGATTPSYDLEHEIDATYPTEHITRELVEKTLKTFVGEIQQIPP 125

Query: 190 MFSAIKVGG 191
            FSA KV G
Sbjct: 126 AFSACKVDG 134

[Close]

BLAST of Cla97C03G060842 vs. ExPASy Swiss-Prot
Match: Q650L8 (tRNA pseudouridine synthase B OS=Bacteroides fragilis (strain YCH46) OX=295405 GN=truB PE=3 SV=1)

HSP 1 Score: 113.2 bits (282), Expect = 3.7e-24
Identity = 60/129 (46.51%), Postives = 79/129 (61.24%), Query Frame = 0


Query: 70  GTVVLLNKPKGWTSFTVCG----KLWRLVKVK--KVGHAGTLDPMATGLLIVCVGKAMKL 129
           G V+  NKP GWTSF V G     + R +KVK  KVGHAGTLDP+ATG++IVC GKA K 
Sbjct: 6   GEVLYFNKPLGWTSFKVVGHARYHMCRRMKVKKLKVGHAGTLDPLATGVMIVCTGKATKR 65

Query: 130 VD--RYQGMSYSGVFRLGEATSTWDADSPVIQREPWEHIKDDDIKKAAASFCGEIWQVPP 189
           ++  +Y    Y    +LG  T ++D +  +    P EHI  + ++K   +F GEI Q+PP
Sbjct: 66  IEEFQYHTKEYVATIQLGATTPSYDLEHEIDATYPTEHITRELVEKTLKTFVGEIQQIPP 125

Query: 190 MFSAIKVGG 191
            FSA KV G
Sbjct: 126 AFSACKVDG 134

[Close]

BLAST of Cla97C03G060842 vs. ExPASy Swiss-Prot
Match: A1R520 (tRNA pseudouridine synthase B OS=Paenarthrobacter aurescens (strain TC1) OX=290340 GN=truB PE=3 SV=1)

HSP 1 Score: 110.9 bits (276), Expect = 1.8e-23
Identity = 57/121 (47.11%), Postives = 76/121 (62.81%), Query Frame = 0


Query: 72  VVLLNKPKGWTSFTVCGKLWRLVKVKKVGHAGTLDPMATGLLIVCVGKAMKLVDRYQGMS 131
           +V+++KP+GWTS  V G++ RL   +KVGHAGTLDPMATG+L++ + KA +L+    G S
Sbjct: 5   LVIVDKPQGWTSHDVVGRMRRLAGTRKVGHAGTLDPMATGVLVLGINKATRLLTYIVGTS 64

Query: 132 --YSGVFRLGEATSTWDADSPVIQREPWEHIKDDDIKKAAASFCGEIWQVPPMFSAIKVG 191
             Y+   RLGE T T DA+  V +     HI DD +    A+  G I QVP   SAIKV 
Sbjct: 65  KTYTATIRLGETTITDDAEGEVTEARTAAHITDDAVAVGVAALTGPIQQVPSSVSAIKVN 124

[Close]

BLAST of Cla97C03G060842 vs. ExPASy Swiss-Prot
Match: A6LEL7 (tRNA pseudouridine synthase B OS=Parabacteroides distasonis (strain ATCC 8503 / DSM 20701 / CIP 104284 / JCM 5825 / NCTC 11152) OX=435591 GN=truB PE=3 SV=1)

HSP 1 Score: 110.9 bits (276), Expect = 1.8e-23
Identity = 59/133 (44.36%), Postives = 82/133 (61.65%), Query Frame = 0


Query: 66  DSPSGTVVLLNKPKGWTSFTVCG----KLWRLVKVK--KVGHAGTLDPMATGLLIVCVGK 125
           D  +G ++  NKP  WTSF +      KL R +KVK  KVGHAGTLDP+ATG++IVC G+
Sbjct: 2   DFIAGEILYFNKPLTWTSFDLVNKFRYKLSRKLKVKKIKVGHAGTLDPLATGVMIVCTGR 61

Query: 126 AMKLVD--RYQGMSYSGVFRLGEATSTWDADSPVIQREPWEHIKDDDIKKAAASFCGEIW 185
           A K +D  +YQ   Y    +LGE T ++D +  +    P EHI  + +++   +F G I 
Sbjct: 62  ATKRIDEFQYQTKEYIATLKLGETTPSFDLEKEIDAVYPTEHITRELVEEVLKTFVGTIQ 121

Query: 186 QVPPMFSAIKVGG 191
           Q+PP+FSA KV G
Sbjct: 122 QIPPVFSACKVEG 134

[Close]

BLAST of Cla97C03G060842 vs. ExPASy Swiss-Prot
Match: Q47RU8 (tRNA pseudouridine synthase B OS=Thermobifida fusca (strain YX) OX=269800 GN=truB PE=3 SV=1)

HSP 1 Score: 110.2 bits (274), Expect = 3.1e-23
Identity = 55/122 (45.08%), Postives = 80/122 (65.57%), Query Frame = 0


Query: 72  VVLLNKPKGWTSFTVCGKLWRLVKVKKVGHAGTLDPMATGLLIVCVGKAMKLVDRYQGMS 131
           VV+++KP GWTS  V  ++ RL   ++VGHAGTLDPMATG+L+V VGKA +L+  Y  ++
Sbjct: 5   VVIVDKPAGWTSHDVVARVRRLAGTRRVGHAGTLDPMATGVLVVGVGKATRLLG-YLALT 64

Query: 132 ---YSGVFRLGEATSTWDADSPVIQREPWEHIKDDDIKKAAASFCGEIWQVPPMFSAIKV 191
              Y    RLG++T+T DA+  +++R P +HI +  +     +  G I QVPP  SA+KV
Sbjct: 65  EKVYEATIRLGQSTTTDDAEGELLERRPADHIDEAAVHAGTRALTGVIHQVPPQVSAVKV 124

[Close]

BLAST of Cla97C03G060842 vs. ExPASy TrEMBL
Match: A0A6J1JSH8 (uncharacterized protein LOC111489372 OS=Cucurbita maxima OX=3661 GN=LOC111489372 PE=3 SV=1)

HSP 1 Score: 364.0 bits (933), Expect = 4.4e-97
Identity = 176/192 (91.67%), Postives = 182/192 (94.79%), Query Frame = 0


Query: 1   MNDKGLVFFDEGKPEKDNKGSVKDVAEKKVEEFFKCLKKVPAKDSEIGQMDPYLLTRHTE 60
           +++KGLVFFD G+PEKDNKGSVKDV EKKVEEFFKCLKKVPAKDSEIGQ +PYLLTRHTE
Sbjct: 255 VDEKGLVFFDGGRPEKDNKGSVKDVTEKKVEEFFKCLKKVPAKDSEIGQTEPYLLTRHTE 314

Query: 61  LPAKWDSPSGTVVLLNKPKGWTSFTVCGKLWRLVKVKKVGHAGTLDPMATGLLIVCVGKA 120
           LPAKWDSP GTVVL+NKPKGWTSFTVCGKL RLVKVKKVGHAGTLDPMATGLLIVCVGKA
Sbjct: 315 LPAKWDSPCGTVVLVNKPKGWTSFTVCGKLRRLVKVKKVGHAGTLDPMATGLLIVCVGKA 374

Query: 121 MKLVDRYQGM--SYSGVFRLGEATSTWDADSPVIQREPWEHIKDDDIKKAAASFCGEIWQ 180
            KL DRYQGM  SYSGVFRLGEATSTWDADS VIQREPWEHIKDDDIKKAAASFCGEIWQ
Sbjct: 375 TKLADRYQGMIKSYSGVFRLGEATSTWDADSAVIQREPWEHIKDDDIKKAAASFCGEIWQ 434

Query: 181 VPPMFSAIKVGG 191
           VPPMFSAIKVGG
Sbjct: 435 VPPMFSAIKVGG 446

[Close]

BLAST of Cla97C03G060842 vs. ExPASy TrEMBL
Match: A0A6J1FDZ7 (uncharacterized protein LOC111444875 OS=Cucurbita moschata OX=3662 GN=LOC111444875 PE=3 SV=1)

HSP 1 Score: 363.6 bits (932), Expect = 5.8e-97
Identity = 176/192 (91.67%), Postives = 181/192 (94.27%), Query Frame = 0


Query: 1   MNDKGLVFFDEGKPEKDNKGSVKDVAEKKVEEFFKCLKKVPAKDSEIGQMDPYLLTRHTE 60
           +++KGLVFFD G+PEKDNKGSVKDV EKKVEEFFKCLKKVPAKDSEIGQM PYLLTRHTE
Sbjct: 255 VDEKGLVFFDGGRPEKDNKGSVKDVTEKKVEEFFKCLKKVPAKDSEIGQMGPYLLTRHTE 314

Query: 61  LPAKWDSPSGTVVLLNKPKGWTSFTVCGKLWRLVKVKKVGHAGTLDPMATGLLIVCVGKA 120
           LPAKWDSP GTVVL+NKPKGWTSFTVCGKL RLVKVKKVGHAGTLDPMATGLLIVCVGKA
Sbjct: 315 LPAKWDSPCGTVVLVNKPKGWTSFTVCGKLRRLVKVKKVGHAGTLDPMATGLLIVCVGKA 374

Query: 121 MKLVDRYQGM--SYSGVFRLGEATSTWDADSPVIQREPWEHIKDDDIKKAAASFCGEIWQ 180
            KL DRYQGM  SYSGVFRLGEATSTWDADS VIQREPWEHIKDDDIKKA ASFCGEIWQ
Sbjct: 375 TKLADRYQGMIKSYSGVFRLGEATSTWDADSAVIQREPWEHIKDDDIKKAVASFCGEIWQ 434

Query: 181 VPPMFSAIKVGG 191
           VPPMFSAIKVGG
Sbjct: 435 VPPMFSAIKVGG 446

[Close]

BLAST of Cla97C03G060842 vs. ExPASy TrEMBL
Match: A0A5A7T7S0 (TruB_N domain-containing protein OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold249G00150 PE=3 SV=1)

HSP 1 Score: 361.3 bits (926), Expect = 2.9e-96
Identity = 174/192 (90.62%), Postives = 181/192 (94.27%), Query Frame = 0


Query: 1   MNDKGLVFFDEGKPEKDNKGSVKDVAEKKVEEFFKCLKKVPAKDSEIGQMDPYLLTRHTE 60
           M+DKG+VFFDEGKPEK+NKGSV DV EKKVEEFFKCLKK PAKDS+IGQ +PYLLTRH E
Sbjct: 256 MDDKGMVFFDEGKPEKENKGSVTDVTEKKVEEFFKCLKKGPAKDSKIGQAEPYLLTRHME 315

Query: 61  LPAKWDSPSGTVVLLNKPKGWTSFTVCGKLWRLVKVKKVGHAGTLDPMATGLLIVCVGKA 120
           LPA+WDSP GTVVLLNKPKGWTSFTVCGKL RLVKV+KVGHAGTLDPMATGLLIVCVGKA
Sbjct: 316 LPARWDSPCGTVVLLNKPKGWTSFTVCGKLRRLVKVQKVGHAGTLDPMATGLLIVCVGKA 375

Query: 121 MKLVDRYQGM--SYSGVFRLGEATSTWDADSPVIQREPWEHIKDDDIKKAAASFCGEIWQ 180
            KLVDRYQGM   YSGVFRLGEATSTWDADSPVIQREPWEHIKDDDIKKAAASFCGEIWQ
Sbjct: 376 TKLVDRYQGMIKGYSGVFRLGEATSTWDADSPVIQREPWEHIKDDDIKKAAASFCGEIWQ 435

Query: 181 VPPMFSAIKVGG 191
           VPPMFSAIKVGG
Sbjct: 436 VPPMFSAIKVGG 447

[Close]

BLAST of Cla97C03G060842 vs. ExPASy TrEMBL
Match: A0A1S3C8B0 (uncharacterized protein LOC103498118 OS=Cucumis melo OX=3656 GN=LOC103498118 PE=3 SV=1)

HSP 1 Score: 361.3 bits (926), Expect = 2.9e-96
Identity = 174/192 (90.62%), Postives = 181/192 (94.27%), Query Frame = 0


Query: 1   MNDKGLVFFDEGKPEKDNKGSVKDVAEKKVEEFFKCLKKVPAKDSEIGQMDPYLLTRHTE 60
           M+DKG+VFFDEGKPEK+NKGSV DV EKKVEEFFKCLKK PAKDS+IGQ +PYLLTRH E
Sbjct: 256 MDDKGMVFFDEGKPEKENKGSVTDVTEKKVEEFFKCLKKGPAKDSKIGQAEPYLLTRHME 315

Query: 61  LPAKWDSPSGTVVLLNKPKGWTSFTVCGKLWRLVKVKKVGHAGTLDPMATGLLIVCVGKA 120
           LPA+WDSP GTVVLLNKPKGWTSFTVCGKL RLVKV+KVGHAGTLDPMATGLLIVCVGKA
Sbjct: 316 LPARWDSPCGTVVLLNKPKGWTSFTVCGKLRRLVKVQKVGHAGTLDPMATGLLIVCVGKA 375

Query: 121 MKLVDRYQGM--SYSGVFRLGEATSTWDADSPVIQREPWEHIKDDDIKKAAASFCGEIWQ 180
            KLVDRYQGM   YSGVFRLGEATSTWDADSPVIQREPWEHIKDDDIKKAAASFCGEIWQ
Sbjct: 376 TKLVDRYQGMIKGYSGVFRLGEATSTWDADSPVIQREPWEHIKDDDIKKAAASFCGEIWQ 435

Query: 181 VPPMFSAIKVGG 191
           VPPMFSAIKVGG
Sbjct: 436 VPPMFSAIKVGG 447

[Close]

BLAST of Cla97C03G060842 vs. ExPASy TrEMBL
Match: A0A0A0KP03 (Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_5G522940 PE=3 SV=1)

HSP 1 Score: 359.8 bits (922), Expect = 8.4e-96
Identity = 175/192 (91.15%), Postives = 181/192 (94.27%), Query Frame = 0


Query: 1   MNDKGLVFFDEGKPEKDNKGSVKDVAEKKVEEFFKCLKKVPAKDSEIGQMDPYLLTRHTE 60
           M+DK +VFFDEGKPEK+NKGSV DV+EKKVEEFFKCLKK PAKDS IGQ +PYLLTRH E
Sbjct: 271 MDDKRMVFFDEGKPEKENKGSVVDVSEKKVEEFFKCLKKGPAKDSNIGQGEPYLLTRHME 330

Query: 61  LPAKWDSPSGTVVLLNKPKGWTSFTVCGKLWRLVKVKKVGHAGTLDPMATGLLIVCVGKA 120
           LPAKWDSP GTVVLLNKPKGWTSFTVCGKL RLVKVKKVGHAGTLDPMATGLLIVCVGKA
Sbjct: 331 LPAKWDSPCGTVVLLNKPKGWTSFTVCGKLRRLVKVKKVGHAGTLDPMATGLLIVCVGKA 390

Query: 121 MKLVDRYQGM--SYSGVFRLGEATSTWDADSPVIQREPWEHIKDDDIKKAAASFCGEIWQ 180
            KLVDRYQGM  SYSGVFRLGEATSTWDADSPVIQREPWEHIKDDDI+KAAASFCGEIWQ
Sbjct: 391 TKLVDRYQGMIKSYSGVFRLGEATSTWDADSPVIQREPWEHIKDDDIQKAAASFCGEIWQ 450

Query: 181 VPPMFSAIKVGG 191
           VPPMFSAIKVGG
Sbjct: 451 VPPMFSAIKVGG 462

[Close]

BLAST of Cla97C03G060842 vs. TAIR 10
Match: AT5G14460.1 (Pseudouridine synthase family protein )

HSP 1 Score: 279.3 bits (713), Expect = 2.8e-75
Identity = 145/197 (73.60%), Postives = 160/197 (81.22%), Query Frame = 0


Query: 2   NDKGLVFFDEGKPEKDNKGSVKD---VAEKKVEEFFKCLKKVPAKD---SEIGQMDPYLL 61
           +D  LVFFDE K +++ K S  D   V EKKVE+FFK L K P +    S  G  +P+L+
Sbjct: 247 DDDKLVFFDEVKEKEEKKKSEDDVVVVTEKKVEQFFKGLTKSPNEKGMASGGGDGEPFLV 306

Query: 62  TRHTELPAKWDSPSGTVVLLNKPKGWTSFTVCGKLWRLVKVKKVGHAGTLDPMATGLLIV 121
           TR+ ELP +WD P+GTV+L+NKPKGWTSFTVCGKL RLVKVKKVGHAGTLDPMATGLLIV
Sbjct: 307 TRNGELPPRWDGPNGTVLLVNKPKGWTSFTVCGKLRRLVKVKKVGHAGTLDPMATGLLIV 366

Query: 122 CVGKAMKLVDRYQGM--SYSGVFRLGEATSTWDADSPVIQREPWEHIKDDDIKKAAASFC 181
           CVGKA K+VDRYQGM   YSGVFRLGEATST DADSPVIQRE WEHIKDDDIKKA  SF 
Sbjct: 367 CVGKATKVVDRYQGMIKGYSGVFRLGEATSTLDADSPVIQRESWEHIKDDDIKKALTSFL 426

Query: 182 GEIWQVPPMFSAIKVGG 191
           GEIWQVPPMFSAIKVGG
Sbjct: 427 GEIWQVPPMFSAIKVGG 443

[Close]

BLAST of Cla97C03G060842 vs. TAIR 10
Match: AT3G57150.1 (homologue of NAP57 )

HSP 1 Score: 67.8 bits (164), Expect = 1.3e-11
Identity = 50/158 (31.65%), Postives = 78/158 (49.37%), Query Frame = 0


Query: 72  VVLLNKPKGWTSFTVCGKLWRLVKVKKVGHAGTLDPMATGLLIVCVGKAMKLVDRYQ--G 131
           V+ L+KP   +S  V   + R+++V+K GH+GTLDP  TG LIVC+ +A +LV   Q  G
Sbjct: 82  VINLDKPANPSSHEVVAWIKRILRVEKTGHSGTLDPKVTGNLIVCIDRATRLVKSQQGAG 141

Query: 132 MSYSGVFRLGEATSTWDADSPVIQREPWEHIKDDDIKKAAASFCGEIWQVPPMFSAIK-- 191
             Y  V RL  A        P + +          + +A  S  G ++Q PP+ SA+K  
Sbjct: 142 KEYVCVARLHSAV-------PDVAK----------VARALESLTGAVFQRPPLISAVKRQ 201

Query: 192 --VGGVGEGMISNIDR-VWLMVMSMSIFRGSYIHNLII 223
             +  + E  +   D    L+V  +S   G+YI  + +
Sbjct: 202 LRIRTIYESKLLEYDADRHLVVFWVSCEAGTYIRTMCV 222

The following BLAST results are available for this feature:

BLAST of Cla97C03G060842 vs. NCBI nr


Match Name	E-value	Identity	Description
XP_038891059.1	1.4e-97	92.71	uncharacterized protein LOC120080471 [Benincasa hispida]	[more]
XP_022993331.1	9.2e-97	91.67	uncharacterized protein LOC111489372 [Cucurbita maxima] >XP_022993332.1 uncharac...	[more]
XP_022938741.1	1.2e-96	91.67	uncharacterized protein LOC111444875 [Cucurbita moschata] >KAG7016215.1 truB [Cu...	[more]
KAG6578676.1	2.7e-96	91.15	hypothetical protein SDJN03_23124, partial [Cucurbita argyrosperma subsp. sorori...	[more]
KAA0037597.1	6.0e-96	90.63	TruB_N domain-containing protein [Cucumis melo var. makuwa] >TYK09336.1 TruB_N d...	[more]

BLAST of Cla97C03G060842 vs. ExPASy Swiss-Prot


Match Name	E-value	Identity	Description
Q5LJ29	3.7e-24	46.51	tRNA pseudouridine synthase B OS=Bacteroides fragilis (strain ATCC 25285 / DSM 2...	[more]
Q650L8	3.7e-24	46.51	tRNA pseudouridine synthase B OS=Bacteroides fragilis (strain YCH46) OX=295405 G...	[more]
A1R520	1.8e-23	47.11	tRNA pseudouridine synthase B OS=Paenarthrobacter aurescens (strain TC1) OX=2903...	[more]
A6LEL7	1.8e-23	44.36	tRNA pseudouridine synthase B OS=Parabacteroides distasonis (strain ATCC 8503 / ...	[more]
Q47RU8	3.1e-23	45.08	tRNA pseudouridine synthase B OS=Thermobifida fusca (strain YX) OX=269800 GN=tru...	[more]

BLAST of Cla97C03G060842 vs. ExPASy TrEMBL


Match Name	E-value	Identity	Description
A0A6J1JSH8	4.4e-97	91.67	uncharacterized protein LOC111489372 OS=Cucurbita maxima OX=3661 GN=LOC111489372...	[more]
A0A6J1FDZ7	5.8e-97	91.67	uncharacterized protein LOC111444875 OS=Cucurbita moschata OX=3662 GN=LOC1114448...	[more]
A0A5A7T7S0	2.9e-96	90.63	TruB_N domain-containing protein OS=Cucumis melo var. makuwa OX=1194695 GN=E5676...	[more]
A0A1S3C8B0	2.9e-96	90.63	uncharacterized protein LOC103498118 OS=Cucumis melo OX=3656 GN=LOC103498118 PE=...	[more]
A0A0A0KP03	8.4e-96	91.15	Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_5G522940 PE=3 SV=1	[more]

BLAST of Cla97C03G060842 vs. TAIR 10


Match Name	E-value	Identity	Description
AT5G14460.1	2.8e-75	73.60	Pseudouridine synthase family protein	[more]
AT3G57150.1	1.3e-11	31.65	homologue of NAP57	[more]

InterPro

Analysis Name: InterPro Annotations of Watermelon (97103) v2.5
Date Performed: 2022-01-31

Position :

Zoom :

x 1


IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
None	No IPR available	GENE3D	3.30.2350.10	Pseudouridine synthase	coord: 63..192 e-value: 7.2E-46 score: 158.4
IPR002501	Pseudouridine synthase II, N-terminal	PFAM	PF01509	TruB_N	coord: 92..190 e-value: 4.7E-33 score: 114.7
IPR014780	tRNA pseudouridine synthase II, TruB	PANTHER	PTHR13767	TRNA-PSEUDOURIDINE SYNTHASE	coord: 44..191
IPR020103	Pseudouridine synthase, catalytic domain superfamily	SUPERFAMILY	55120	Pseudouridine synthase	coord: 71..220

Relationships

The following mRNA feature(s) are a part of this gene:


Feature Name	Unique Name	Type
Cla97C03G060842.1	Cla97C03G060842.1	mRNA

GO Annotation

GO Assignments

This gene is annotated with the following GO terms.

Category	Term Accession	Term Name
biological_process	GO:1990481	mRNA pseudouridine synthesis
biological_process	GO:0006400	tRNA modification
biological_process	GO:0001522	pseudouridine synthesis
biological_process	GO:0009451	RNA modification
biological_process	GO:0006396	RNA processing
cellular_component	GO:0005739	mitochondrion
molecular_function	GO:0009982	pseudouridine synthase activity
molecular_function	GO:0003723	RNA binding