Cla97C03G059780 (gene) Watermelon (97103) v2.5

Overview
NameCla97C03G059780
Typegene
OrganismCitrullus lanatus subsp. vulgaris cv. 97103 (Watermelon (97103) v2.5)
Descriptionprimase homolog protein isoform X2
LocationCla97Chr03: 9616551 .. 9622275 (-)
RNA-Seq ExpressionCla97C03G059780
SyntenyCla97C03G059780
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: polypeptideexonCDS
Hold the cursor over a type above to highlight its positions in the sequence below.
ATGCCAATAAACTCCACTTTCACTTTCAATTTCAATTTCAACTTCAACTTCAACTTCAACTTCTATCATCGCTCTCCCCCTTTTTCTCATCCACCAAACGATTTCTCCTTCTCCTCTTCCCCTTCCCGCTTCCTTCGATTCTGCACTACCAGACTAGCTTCCGCCGTTTTTAATGCTTCGCCGTCGCCACCACCAGGTTTTCGTTCTTTTCTAGACTCTGTTGAAACTGAAACCCCTCTTTTGTTTGTTCTCGGATTTTGGGTTAATGTTTTGGGCTACCCTAAAGTGGAGGTTCTAAGGGAGAAGATGGATCTTATCGGAATCGTCTGTGATGAACATTGTACTCCTGGAAAATACTATTGCCTCTTCTGTCCCAAGGTTATCTGCTCCTCTCGTTTCTACATTTCGTGGTTTGAATCAAATACATGAAAATCCAAACCCATCTTTTCATTTATATTGTCTTTTTGGTTTTCGTTAGATTATAGGTTAGAGACAAGATTTTCTTGATTTTTTTTTAAAAGTTCAAGGGCAACATACGGTTTAAAAGGTTGGAGATAGTAATAGAACAAACTTAAAAGTTACGGGACAAAAAGGAAACAGACTTAGAAAGTTTAGAGACCAAAATGGTATTTAAACCTAATTTATATATTTCAAGAAGTTCCTAACAAAGTTTTATGGTTAGATTACATATTTGGTCCCTATGGTTTGGAGAAAGTTAGAGTTTAGTCTTATAGTTTTAAATGTTATAATTTAGTACTTGTGGTTTGATAAAACCTCGTAAATAATCTTTATGGTTTGATAAAATCTTCATAAATATCAATCATTCAGAACCACTAAGGAAGGATTCTCAGGACTATTTATAAATGTTGAATGATTTAATATTAAATTTGTCTTCACCTACTAGCTTAAGCTTTTGGGTCAATCGGCGATTTAACAATAAAGGTTTTATCAAACCATAAGTGTAAATTCCAATTATAAACCATAGAGGCTAGATTCTAACTTTCTCCATTCTAAAGGGTCCAAATTTACAATTTAACCAAGTTTTTTATATTTGACTCATATACTTTAAAAAGTTTATAATATGCTATTAACAGTTATGTCTCCACATCCGTGATCCTCCCAACATCATCTCTGTTCATAGTTTTTCAAGAATCTAGCCAGTATGGCAAAATAAGTACTAAATTGTCAAATACTAACGACACAGACTTGAAAAATTCAAGGACCTATTTGAAATCTTTCTAAATATACTAGGACTAAATAGACACAATATGTTGAAACCTCTTAGGAGCCAAATAAGCATAACTATTGAAGTTGGGAGACCAAATTTATAAGTTAACCTTCTTTTATTACTATCTCTCTAATTAGATCTATTCATGGTTGAACAGTGCAAGGGTGGTCGTTTTATGGAGAGGAGCTTATCTCTTCATGTGATTCCAACTGGGTAAGTGATAATATTACCATACTGGTACTATTTGTTCTTTCTTCTTCATTTGTATGGAGATTCATTGGGGAGTGAATTGTTGTTAATTACATTTTGTCATTGGGAACATAGAGATTTTGCAATGTGGAGGTGTTTCCAGTCTGAGTGTGGTTGGGCAGGCCGGGTAAAGTTATAATAATAACGTGTATTTGTAATATTCTCTTCAATCTCATATCTAGCAATTAAACATGAGAATGTTGCTCAGCCACATGATTGTTTCGTTTAGATATTTGCAGATGGCAGATTGGCATTTAATGGATTCAGCAGAACTATAAAGAGGGTTGGATGGAGTGCAAAGGAAAGTTTGGTTTTGGAGCCTCTTTGTGATGAGGTACTTCAAATTTCATTACATTTCCATGAATAAGGCATGCTTTCACACTAATATACCTCAGTGTTGGTTCAATGGGTTAACTGATGCAATCTTTTGACATAAACATAAGTTATTGGTTCAATAGGAAATTGAGTTAGCTCTTGATTTGATTCCTAGTGAGTTGTACAGGTTACAATGAATAGAAAATACTTCTAACTTAATTCGAGAGTGTTTTGATCTTTCTTTTTCAGCTAATCTCTTACTTCAGCAAGAGAATGATATCCCAAGAAACCTTAAAAAGGAATGTTGTTATGCAAATAGCTGGTCGTCAGGTACCTGAAACAATGTTTTTGTGTGTATGCCTTATAATTGAAATTTTAGACTTGGCTTCTTGTGGATGCTATGCTCACTAGTTTCATTGTTAGTATTGACTCGAACATTATTCAATCTATATGTTATTTGTATGTCATGGTGTCTTCGATGAATCATTCCCAAAGCCGATGACAATTTATTTGTTCGAAATGTGTTGCATTCTTTTGTTTATATAATTAGGTGTTTGGTGTTTTAGAACCTCTCCTCATTGAAGAAAGGTTCTTACTTCTAAACTGCGGATGGATATCCTCACCTTATGGGTGAATGTTTCTCTATTGAGGTCCATCATTATAGAATAGGCTTTCAATTAGTGTTTAAATGGTGATTATTCTCTCTCTTTCCCTTGTAGGTTTAACATCTAATAAGTTTAAAGGTGACTTGCAGGTTGCTATTGCATTTACTTATAGACAAAATGGACAGCTCATCGGTTGCAAGTACCGAACCATGGACAAGAGATTTTGGCAGGTACGTAACCCTTCTTCTATCAGAGTTATTTGAGAAATTCTCAGAATGTTCCAGCATCTTTCATTCTGTTCTTTGGCTTATAATTTTTAAGTGTTTATTTCTTGTGCACAGGTTACTTTTCATTTGTTGCCCTCTTTAGCCTTTAGACATAGAACAGATCTTTTATACAGAGAATCTAATCTGTATGTCTGATGTTAACATTCAGGAGAAGGGTACAGAAAAGTTATTGTATGGGATTGATGACATTAATGATGCAGATGAACTCATCATTGTAATCCACATTACCTTATCTTTTAAGGTTCTATTTGATATTTTAATCGCTTTTTCCGTCCATTTTATGTAATATTATGGATATATTGTTCAAGGTTGAAGGTGAAATGGATAAACTTTCAGTGGAAGAAGCTGGTTTCCTCAATTGTATCAGTGTTCCTAGTGGTGCCCCAAGCAAGGTTTCAACTGATACAGTTCCATCAATTGAAAAGGTTCTATTTTATACAAATAGTCCTATGCCTGTACTGTGTGCTTTACATACATTCTTCCTAACTGTCCCAGTTATGCTGCAAATTACCAATTTTGATCTTCCCTTTGAAAATAAACTTTTAGTTGATCTTTGAAAAATTACACAAGTCACTACAGCATCATATAATAAACGCTCTACACCCCTTCTCAACTAGAAACTAACTAAAAAACTAGAAATGATAGAGTAAATTATAATAAACCACCCAAATAGAGAATTTATTACATTACAAGAATAACTAGAATAGATTCTAGATTGAGAACACCAAAGACATAAGCCCTATTTGATAACCATTTGAATTTTTGTTTTTGGTTTTCGAAACTTGTGTTGGTTTACTCACAAATTCTTGAACATTGTTTTCATCTTTTGAAGTTAACGTTTGAATTCTTAGCCAAATTCAAAAAAAGATGAACAGAATTTTAAAAAATCAATTTTTGTAGTTTTGAAAACATGACTTGAATTTTTGGAAGCACTACTGAAAAGTAGATAATAAAACAAAAAAACCAATAGGCGGAAGTATTGTTTCTACAAGCTTTTCAAAACTAAATAGTTATCACATGGGGTCAGAGAGAACTGATTCTAACCTTTAGTTTTTGTGTCAATATTACATTTAGTTTTAGTGATTACATATGGTTTATAAATTCATTGTTTTTTAACTTATATTGTATAACATATGGAAACTGTTTAGTGTACTTCAACTTATTCTATATGCATATTTTGGTGGGATGAAGTCAGGATACTGCATACCAATTTTTATGGAGCTGGAAAGACTACTTAGATAAGGTAACATTGTGCCTGTTAGATATAACCTTTTTGCTTTTACCTTTTAGTGTTAAAAACAATCTTGTTATATATAGTTTCGTTTCTTCATTTGCCTTGTTAAGTTTGAAACACTAACAAAGTAATTAACCTACAAGGTTTCCCGCATTATCTTGGCTACTGATAGCGATGAACCGGGGCAAGCTCTGGCAGAAGAGCTTGCACGTCGCCTTGGAAAACACAGGTCTCAACTCATTTGTGAACCTTTTGTTAATTTTAAAATCAAGATCAAAATTCTATTCCCGTCAAAATGATCCTGGCTTAACTAATATAAAATTTGTACTATCGACCTTGAGGCTAGAGGTTTGATTCTCCCAACCTACATATTGTCAATAAAATAATAATAATAATCAAACTTCTATACTCGTAATGAATGCTTCAGTTTTATTTTATTTTTTATTATATATTTTTTCCCCTTCATGTAGACAACATGGCTTTGTTTTATGGATTAACAGATGTTGGCGAGTAGACTGGCCATATAAAGATGACATCAACCGTTTTAAAGATGCCAATGAGGTTACCTTCCTGCTTTGAAATTACTATAGTTATTCATAAAGTCCTTGCACCTACAAAAGTTGCCCATGACTTGTATACTAGGAATGAATGATCAATACAATAAAATTAATCTTGATGATTGAGTATAAATAGAGGTGGGGCTTGGTCATTAACACATATTTTATGTCAGTTGAGTTAAGTTTACTTCGACAATTAATCTTGATGATTGGGTGGATATGAAATTGTCCACTTAAAAAACAAGCCTAGACAGTTGTTTTCAGAATCTAACTAACATTCTTCATATGGATTGAAGTATTATTTTTACTTTTAAACTAAAGCTTTTTTAATCATTGATAGTAATATGACTCTTGTAGCTAGCCCTGGATAAAGTAAGACAAAAGTCAAAGTATCAAATAGGAATATATTAGTAAGTAATTAGAGTATTAAGGGCATATTAGCAATTAGCTATGGAGTTTGTTAGGAAAGTATGGTTATAAAAAAGTGTGCGGGGAAGGAGATAGGCAATATTTTTGAGTATGAATTAAGGCTTGAGAGTATTCTCAAGATTGGGAGGGTTCATGTATCTCGAACGCTTGGTTTATCTTGTAATTTCATTATAGTTCAGTATATTCAGGTTATTCTAGTTATTCTAATTTCTCTCCCTTCTCATTGTTCATTCTTAGTTCCAAGCTTCTGTCTTCCTTTTAATCCTTTTTCTTCATTTCATTTTTTATCTTGTGTGTGTGTTCTAAATTATTAAATTATATATATACACATATGAACAGATTCTAATGCACTTGGGGCCAGATGCTTTGAAGAACGCAATTCAAGATGCCAAGTTATATCAACTTTCTAATTCAAATCAAATACAAAAAGGTGTGTACTTAATATTTCAAGAGCTTATCCCCATGATATTCATTGGATGGCATATATTTAACATTCATTTCAAAAAATGTTGACTTCTTTTATGGATATTTTATATTATATTTTAATTTGAGTTAGGTTTTGATAGCTCTACCTTTTTAAGATTTTAAACTTCTTTCCATACTTTATAAACATATTTATACTATGGGTAATTTAGGTAAATTACCTCTCTCTCCCTCCCTTATTTTTATTGCAATGGTTTATAAATGCTTCCTGTAATAGACTCTTTTTGCTTTTCCATTTATTACTTCATGTTCTCTTTTTACTTTTGGTAATTATTTTGATAACCTTCTCCCCTTTTGACTTTGCAGAAGAGGACCTCAAGAGCTTCCAAGAGCCTTAA

mRNA sequence

ATGCCAATAAACTCCACTTTCACTTTCAATTTCAATTTCAACTTCAACTTCAACTTCAACTTCTATCATCGCTCTCCCCCTTTTTCTCATCCACCAAACGATTTCTCCTTCTCCTCTTCCCCTTCCCGCTTCCTTCGATTCTGCACTACCAGACTAGCTTCCGCCGTTTTTAATGCTTCGCCGTCGCCACCACCAGGTTTTCGTTCTTTTCTAGACTCTGTTGAAACTGAAACCCCTCTTTTGTTTGTTCTCGGATTTTGGGTTAATGTTTTGGGCTACCCTAAAGTGGAGGTTCTAAGGGAGAAGATGGATCTTATCGGAATCGTCTGTGATGAACATTGTACTCCTGGAAAATACTATTGCCTCTTCTGTCCCAAGTGCAAGGGTGGTCGTTTTATGGAGAGGAGCTTATCTCTTCATGTGATTCCAACTGGAGATTTTGCAATGTGGAGGTGTTTCCAGTCTGAGTGTGGTTGGGCAGGCCGGATATTTGCAGATGGCAGATTGGCATTTAATGGATTCAGCAGAACTATAAAGAGGGTTGGATGGAGTGCAAAGGAAAGTTTGGTTTTGGAGCCTCTTTGTGATGAGCTAATCTCTTACTTCAGCAAGAGAATGATATCCCAAGAAACCTTAAAAAGGAATGTTGTTATGCAAATAGCTGGTCGTCAGGTTGCTATTGCATTTACTTATAGACAAAATGGACAGCTCATCGGTTGCAAGTACCGAACCATGGACAAGAGATTTTGGCAGGAGAAGGGTACAGAAAAGTTATTGTATGGGATTGATGACATTAATGATGCAGATGAACTCATCATTGTTGAAGGTGAAATGGATAAACTTTCAGTGGAAGAAGCTGGTTTCCTCAATTGTATCAGTGTTCCTAGTGGTGCCCCAAGCAAGGTTTCAACTGATACAGTTCCATCAATTGAAAAGGATACTGCATACCAATTTTTATGGAGCTGGAAAGACTACTTAGATAAGGTTTCCCGCATTATCTTGGCTACTGATAGCGATGAACCGGGGCAAGCTCTGGCAGAAGAGCTTGCACGTCGCCTTGGAAAACACAGATGTTGGCGAGTAGACTGGCCATATAAAGATGACATCAACCGTTTTAAAGATGCCAATGAGATTCTAATGCACTTGGGGCCAGATGCTTTGAAGAACGCAATTCAAGATGCCAAGTTATATCAACTTTCTAATTCAAATCAAATACAAAAAGAAGAGGACCTCAAGAGCTTCCAAGAGCCTTAA

Coding sequence (CDS)

ATGCCAATAAACTCCACTTTCACTTTCAATTTCAATTTCAACTTCAACTTCAACTTCAACTTCTATCATCGCTCTCCCCCTTTTTCTCATCCACCAAACGATTTCTCCTTCTCCTCTTCCCCTTCCCGCTTCCTTCGATTCTGCACTACCAGACTAGCTTCCGCCGTTTTTAATGCTTCGCCGTCGCCACCACCAGGTTTTCGTTCTTTTCTAGACTCTGTTGAAACTGAAACCCCTCTTTTGTTTGTTCTCGGATTTTGGGTTAATGTTTTGGGCTACCCTAAAGTGGAGGTTCTAAGGGAGAAGATGGATCTTATCGGAATCGTCTGTGATGAACATTGTACTCCTGGAAAATACTATTGCCTCTTCTGTCCCAAGTGCAAGGGTGGTCGTTTTATGGAGAGGAGCTTATCTCTTCATGTGATTCCAACTGGAGATTTTGCAATGTGGAGGTGTTTCCAGTCTGAGTGTGGTTGGGCAGGCCGGATATTTGCAGATGGCAGATTGGCATTTAATGGATTCAGCAGAACTATAAAGAGGGTTGGATGGAGTGCAAAGGAAAGTTTGGTTTTGGAGCCTCTTTGTGATGAGCTAATCTCTTACTTCAGCAAGAGAATGATATCCCAAGAAACCTTAAAAAGGAATGTTGTTATGCAAATAGCTGGTCGTCAGGTTGCTATTGCATTTACTTATAGACAAAATGGACAGCTCATCGGTTGCAAGTACCGAACCATGGACAAGAGATTTTGGCAGGAGAAGGGTACAGAAAAGTTATTGTATGGGATTGATGACATTAATGATGCAGATGAACTCATCATTGTTGAAGGTGAAATGGATAAACTTTCAGTGGAAGAAGCTGGTTTCCTCAATTGTATCAGTGTTCCTAGTGGTGCCCCAAGCAAGGTTTCAACTGATACAGTTCCATCAATTGAAAAGGATACTGCATACCAATTTTTATGGAGCTGGAAAGACTACTTAGATAAGGTTTCCCGCATTATCTTGGCTACTGATAGCGATGAACCGGGGCAAGCTCTGGCAGAAGAGCTTGCACGTCGCCTTGGAAAACACAGATGTTGGCGAGTAGACTGGCCATATAAAGATGACATCAACCGTTTTAAAGATGCCAATGAGATTCTAATGCACTTGGGGCCAGATGCTTTGAAGAACGCAATTCAAGATGCCAAGTTATATCAACTTTCTAATTCAAATCAAATACAAAAAGAAGAGGACCTCAAGAGCTTCCAAGAGCCTTAA

Protein sequence

MPINSTFTFNFNFNFNFNFNFYHRSPPFSHPPNDFSFSSSPSRFLRFCTTRLASAVFNASPSPPPGFRSFLDSVETETPLLFVLGFWVNVLGYPKVEVLREKMDLIGIVCDEHCTPGKYYCLFCPKCKGGRFMERSLSLHVIPTGDFAMWRCFQSECGWAGRIFADGRLAFNGFSRTIKRVGWSAKESLVLEPLCDELISYFSKRMISQETLKRNVVMQIAGRQVAIAFTYRQNGQLIGCKYRTMDKRFWQEKGTEKLLYGIDDINDADELIIVEGEMDKLSVEEAGFLNCISVPSGAPSKVSTDTVPSIEKDTAYQFLWSWKDYLDKVSRIILATDSDEPGQALAEELARRLGKHRCWRVDWPYKDDINRFKDANEILMHLGPDALKNAIQDAKLYQLSNSNQIQKEEDLKSFQEP
Homology
BLAST of Cla97C03G059780 vs. NCBI nr
Match: XP_038893950.1 (primase homolog protein isoform X1 [Benincasa hispida])

HSP 1 Score: 688.3 bits (1775), Expect = 4.0e-194
Identity = 346/416 (83.17%), Postives = 362/416 (87.02%), Query Frame = 0

Query: 3   INSTFTFNFNFNFNFNFNFYHRSPPFSHPPNDFS--FSSSPSRFLRFCTTRLASAVFNAS 62
           ++S  TFN N NFNFN NF HRSPPFSHP NDFS  FSSSPSRFLRFCTTR +SAV +  
Sbjct: 1   MSSNTTFNLNLNFNFN-NFNHRSPPFSHPSNDFSFFFSSSPSRFLRFCTTRPSSAVSHGP 60

Query: 63  PSPPPGFRSFLDSVETETPLLFVLGFWVNVLGYPKVEVLREKMDLIGIVCDEHCTPGKYY 122
           PSPPP                              V+VL EKMDLIGIVCDEHCTPGKYY
Sbjct: 61  PSPPP------------------------------VKVLMEKMDLIGIVCDEHCTPGKYY 120

Query: 123 CLFCPKCKGGRFMERSLSLHVIPTGDFAMWRCFQSECGWAGRIFADGRLAFNGFSRTIKR 182
           CLFCPKCKGGRF+ERSLSLHVIPTGDFAMWRCFQSECGWAGRIFADGRLAFNGFSRT+K 
Sbjct: 121 CLFCPKCKGGRFIERSLSLHVIPTGDFAMWRCFQSECGWAGRIFADGRLAFNGFSRTVKT 180

Query: 183 VGWSAKESLVLEPLCDELISYFSKRMISQETLKRNVVMQIAGRQVAIAFTYRQNGQLIGC 242
           VG SA+ESLVLEPL DELISYF+KRMISQETL+RNVVMQIAGRQVAIAFTYRQNGQL+GC
Sbjct: 181 VGRSARESLVLEPLGDELISYFNKRMISQETLERNVVMQIAGRQVAIAFTYRQNGQLVGC 240

Query: 243 KYRTMDKRFWQEKGTEKLLYGIDDINDADELIIVEGEMDKLSVEEAGFLNCISVPSGAPS 302
           KYRTMDKRFWQEKGTEKLLYGIDDINDA+ELIIVEGEMDKLSVEEAG LNCISVP GAPS
Sbjct: 241 KYRTMDKRFWQEKGTEKLLYGIDDINDANELIIVEGEMDKLSVEEAGLLNCISVPGGAPS 300

Query: 303 KVSTDTVPSIEKDTAYQFLWSWKDYLDKVSRIILATDSDEPGQALAEELARRLGKHRCWR 362
           KVSTDTVPS EKDTAYQ+LWS KDYLDKVSRIILATDSDEPGQALAEELARRLGKHRCWR
Sbjct: 301 KVSTDTVPSNEKDTAYQYLWSCKDYLDKVSRIILATDSDEPGQALAEELARRLGKHRCWR 360

Query: 363 VDWPYKDDINRFKDANEILMHLGPDALKNAIQDAKLYQLSNSNQIQKEEDLKSFQE 417
           VDWPYKDD NRFKDANEILMHLGPDALKNAIQDAK Y+L +SNQI+KEEDLKSFQE
Sbjct: 361 VDWPYKDDFNRFKDANEILMHLGPDALKNAIQDAKFYELFSSNQIKKEEDLKSFQE 385

BLAST of Cla97C03G059780 vs. NCBI nr
Match: XP_011659247.1 (primase homolog protein isoform X1 [Cucumis sativus] >KGN65952.1 hypothetical protein Csa_023223 [Cucumis sativus])

HSP 1 Score: 655.6 bits (1690), Expect = 2.9e-184
Identity = 328/416 (78.85%), Postives = 349/416 (83.89%), Query Frame = 0

Query: 1   MPINSTFTFNFNFNFNFNFNFYHRSPPFSHPPNDFSFSSSPSRFLRFCTTRLASAVFNAS 60
           MPINSTFTFN NFNF F F   HRS PFSH  N+FS SSSPSRFLRFCTTRL+SAV +A 
Sbjct: 1   MPINSTFTFNSNFNFQFKF-INHRSFPFSHSLNNFSISSSPSRFLRFCTTRLSSAVSHAP 60

Query: 61  PSPPPGFRSFLDSVETETPLLFVLGFWVNVLGYPKVEVLREKMDLIGIVCDEHCTPGKYY 120
           PSPPP                              V+ L E+MDL+GIVCDEHC PGKYY
Sbjct: 61  PSPPP------------------------------VKHLMERMDLVGIVCDEHCIPGKYY 120

Query: 121 CLFCPKCKGGRFMERSLSLHVIPTGDFAMWRCFQSECGWAGRIFADGRLAFNGFSRTIKR 180
           CLFCPKCKGGR MERSLSLHVIPTGDFAMWRCFQ ECGWAG+IFADG+LAFNGF +  K 
Sbjct: 121 CLFCPKCKGGRLMERSLSLHVIPTGDFAMWRCFQFECGWAGQIFADGKLAFNGFGKITKM 180

Query: 181 VGWSAKESLVLEPLCDELISYFSKRMISQETLKRNVVMQIAGRQVAIAFTYRQNGQLIGC 240
           VG S+KESLVLEPLCDELISYFS RMISQETL+RNVVMQ+AGRQV IAFTYRQNGQL+GC
Sbjct: 181 VGRSSKESLVLEPLCDELISYFSMRMISQETLERNVVMQLAGRQVTIAFTYRQNGQLVGC 240

Query: 241 KYRTMDKRFWQEKGTEKLLYGIDDINDADELIIVEGEMDKLSVEEAGFLNCISVPSGAPS 300
           KYRTMDKRFWQEKGT K LYGIDDINDADELIIVEGEMDKLSVEEAGFLNCISVP GAP+
Sbjct: 241 KYRTMDKRFWQEKGTAKFLYGIDDINDADELIIVEGEMDKLSVEEAGFLNCISVPGGAPN 300

Query: 301 KVSTDTVPSIEKDTAYQFLWSWKDYLDKVSRIILATDSDEPGQALAEELARRLGKHRCWR 360
           KVSTDTVP IEKDTAYQ+LWS KDYLDKVSR+ILATDSD PGQALAEELARRLGK+RCWR
Sbjct: 301 KVSTDTVPPIEKDTAYQYLWSCKDYLDKVSRVILATDSDVPGQALAEELARRLGKNRCWR 360

Query: 361 VDWPYKDDINRFKDANEILMHLGPDALKNAIQDAKLYQLSNSNQIQKEEDLKSFQE 417
           V WPYKD  NRFKDANE+L+HLGPDALK AI+DAK YQLS+SNQIQKEEDL  FQE
Sbjct: 361 VSWPYKDGFNRFKDANEVLVHLGPDALKKAIEDAKFYQLSSSNQIQKEEDL--FQE 383

BLAST of Cla97C03G059780 vs. NCBI nr
Match: XP_008445790.1 (PREDICTED: primase homolog protein isoform X2 [Cucumis melo])

HSP 1 Score: 652.5 bits (1682), Expect = 2.4e-183
Identity = 332/417 (79.62%), Postives = 348/417 (83.45%), Query Frame = 0

Query: 1   MPINSTFTFNFNFNFNFNFNFYHRSPPFSHPPNDFSFSSSPSRF-LRFCTTRLASAVFNA 60
           MPINSTFT  FN NFNFNF F HRS P S   N+FS SSSPSRF LRFCTTRL+SA+ +A
Sbjct: 1   MPINSTFT--FNSNFNFNFKFIHRSSPSSLSLNNFSISSSPSRFLLRFCTTRLSSALSHA 60

Query: 61  SPSPPPGFRSFLDSVETETPLLFVLGFWVNVLGYPKVEVLREKMDLIGIVCDEHCTPGKY 120
            P PPP                              V+VLREKMDLIGIVCDEHC PGKY
Sbjct: 61  PPLPPP------------------------------VKVLREKMDLIGIVCDEHCIPGKY 120

Query: 121 YCLFCPKCKGGRFMERSLSLHVIPTGDFAMWRCFQSECGWAGRIFADGRLAFNGFSRTIK 180
           YCLFCPKCKGGR MERSLSLHVIPTGDFAMWRCF+ ECGWAGRIFADGRLAFNGFS+T K
Sbjct: 121 YCLFCPKCKGGRLMERSLSLHVIPTGDFAMWRCFRFECGWAGRIFADGRLAFNGFSKTEK 180

Query: 181 RVGWSAKESLVLEPLCDELISYFSKRMISQETLKRNVVMQIAGRQVAIAFTYRQNGQLIG 240
            VG S+KESLVLEPLCDELISYFS RMISQETL+RNVVMQ+ GRQVAIAFTYRQNGQL+G
Sbjct: 181 MVGRSSKESLVLEPLCDELISYFSMRMISQETLERNVVMQVVGRQVAIAFTYRQNGQLVG 240

Query: 241 CKYRTMDKRFWQEKGTEKLLYGIDDINDADELIIVEGEMDKLSVEEAGFLNCISVPSGAP 300
           CKYRTMDKRFWQEKGT K LYGIDDINDADELIIVEGEMDKLSVEEAGFLNCISVP GAP
Sbjct: 241 CKYRTMDKRFWQEKGTAKFLYGIDDINDADELIIVEGEMDKLSVEEAGFLNCISVPGGAP 300

Query: 301 SKVSTDTVPSIEKDTAYQFLWSWKDYLDKVSRIILATDSDEPGQALAEELARRLGKHRCW 360
           SKVSTDT+P IEKD+AYQ+LW  KDYLDKVSRIILATDSD PGQALAEELARRLGKHRCW
Sbjct: 301 SKVSTDTIPPIEKDSAYQYLWGCKDYLDKVSRIILATDSDVPGQALAEELARRLGKHRCW 360

Query: 361 RVDWPYKDDINRFKDANEILMHLGPDALKNAIQDAKLYQLSNSNQIQKEEDLKSFQE 417
           RVDWPYKD  NRFKDANE+L+HLGPDALKNAIQDAK YQLS S  IQKEE L  FQE
Sbjct: 361 RVDWPYKDGFNRFKDANEVLVHLGPDALKNAIQDAKFYQLSISISIQKEEGL--FQE 383

BLAST of Cla97C03G059780 vs. NCBI nr
Match: XP_016899150.1 (PREDICTED: primase homolog protein isoform X1 [Cucumis melo])

HSP 1 Score: 647.9 bits (1670), Expect = 6.0e-182
Identity = 332/418 (79.43%), Postives = 348/418 (83.25%), Query Frame = 0

Query: 1   MPINSTFTFNFNFNFNFNFNFYHRSPPFSHPPNDFSFSSSPSRF-LRFCTTRLASAVFNA 60
           MPINSTFT  FN NFNFNF F HRS P S   N+FS SSSPSRF LRFCTTRL+SA+ +A
Sbjct: 1   MPINSTFT--FNSNFNFNFKFIHRSSPSSLSLNNFSISSSPSRFLLRFCTTRLSSALSHA 60

Query: 61  SPSPPPGFRSFLDSVETETPLLFVLGFWVNVLGYPKVEVLREKMDLIGIVCDEHCTPGKY 120
            P PPP                              V+VLREKMDLIGIVCDEHC PGKY
Sbjct: 61  PPLPPP------------------------------VKVLREKMDLIGIVCDEHCIPGKY 120

Query: 121 YCLFCPKCKGGRFMERSLSLHVIPTGDFAMWRCFQSECGWAGRIFADGRLAFNGFSRTIK 180
           YCLFCPKCKGGR MERSLSLHVIPTGDFAMWRCF+ ECGWAGRIFADGRLAFNGFS+T K
Sbjct: 121 YCLFCPKCKGGRLMERSLSLHVIPTGDFAMWRCFRFECGWAGRIFADGRLAFNGFSKTEK 180

Query: 181 RVGWSAKESLVLEPLCDELISYFSKRMISQETLKRNVVMQIAGR-QVAIAFTYRQNGQLI 240
            VG S+KESLVLEPLCDELISYFS RMISQETL+RNVVMQ+ GR QVAIAFTYRQNGQL+
Sbjct: 181 MVGRSSKESLVLEPLCDELISYFSMRMISQETLERNVVMQVVGRQQVAIAFTYRQNGQLV 240

Query: 241 GCKYRTMDKRFWQEKGTEKLLYGIDDINDADELIIVEGEMDKLSVEEAGFLNCISVPSGA 300
           GCKYRTMDKRFWQEKGT K LYGIDDINDADELIIVEGEMDKLSVEEAGFLNCISVP GA
Sbjct: 241 GCKYRTMDKRFWQEKGTAKFLYGIDDINDADELIIVEGEMDKLSVEEAGFLNCISVPGGA 300

Query: 301 PSKVSTDTVPSIEKDTAYQFLWSWKDYLDKVSRIILATDSDEPGQALAEELARRLGKHRC 360
           PSKVSTDT+P IEKD+AYQ+LW  KDYLDKVSRIILATDSD PGQALAEELARRLGKHRC
Sbjct: 301 PSKVSTDTIPPIEKDSAYQYLWGCKDYLDKVSRIILATDSDVPGQALAEELARRLGKHRC 360

Query: 361 WRVDWPYKDDINRFKDANEILMHLGPDALKNAIQDAKLYQLSNSNQIQKEEDLKSFQE 417
           WRVDWPYKD  NRFKDANE+L+HLGPDALKNAIQDAK YQLS S  IQKEE L  FQE
Sbjct: 361 WRVDWPYKDGFNRFKDANEVLVHLGPDALKNAIQDAKFYQLSISISIQKEEGL--FQE 384

BLAST of Cla97C03G059780 vs. NCBI nr
Match: XP_038893951.1 (primase homolog protein isoform X2 [Benincasa hispida])

HSP 1 Score: 624.8 bits (1610), Expect = 5.4e-175
Identity = 312/378 (82.54%), Postives = 326/378 (86.24%), Query Frame = 0

Query: 3   INSTFTFNFNFNFNFNFNFYHRSPPFSHPPNDFS--FSSSPSRFLRFCTTRLASAVFNAS 62
           ++S  TFN N NFNFN NF HRSPPFSHP NDFS  FSSSPSRFLRFCTTR +SAV +  
Sbjct: 1   MSSNTTFNLNLNFNFN-NFNHRSPPFSHPSNDFSFFFSSSPSRFLRFCTTRPSSAVSHGP 60

Query: 63  PSPPPGFRSFLDSVETETPLLFVLGFWVNVLGYPKVEVLREKMDLIGIVCDEHCTPGKYY 122
           PSPPP                              V+VL EKMDLIGIVCDEHCTPGKYY
Sbjct: 61  PSPPP------------------------------VKVLMEKMDLIGIVCDEHCTPGKYY 120

Query: 123 CLFCPKCKGGRFMERSLSLHVIPTGDFAMWRCFQSECGWAGRIFADGRLAFNGFSRTIKR 182
           CLFCPKCKGGRF+ERSLSLHVIPTGDFAMWRCFQSECGWAGRIFADGRLAFNGFSRT+K 
Sbjct: 121 CLFCPKCKGGRFIERSLSLHVIPTGDFAMWRCFQSECGWAGRIFADGRLAFNGFSRTVKT 180

Query: 183 VGWSAKESLVLEPLCDELISYFSKRMISQETLKRNVVMQIAGRQVAIAFTYRQNGQLIGC 242
           VG SA+ESLVLEPL DELISYF+KRMISQETL+RNVVMQIAGRQVAIAFTYRQNGQL+GC
Sbjct: 181 VGRSARESLVLEPLGDELISYFNKRMISQETLERNVVMQIAGRQVAIAFTYRQNGQLVGC 240

Query: 243 KYRTMDKRFWQEKGTEKLLYGIDDINDADELIIVEGEMDKLSVEEAGFLNCISVPSGAPS 302
           KYRTMDKRFWQEKGTEKLLYGIDDINDA+ELIIVEGEMDKLSVEEAG LNCISVP GAPS
Sbjct: 241 KYRTMDKRFWQEKGTEKLLYGIDDINDANELIIVEGEMDKLSVEEAGLLNCISVPGGAPS 300

Query: 303 KVSTDTVPSIEKDTAYQFLWSWKDYLDKVSRIILATDSDEPGQALAEELARRLGKHRCWR 362
           KVSTDTVPS EKDTAYQ+LWS KDYLDKVSRIILATDSDEPGQALAEELARRLGKHRCWR
Sbjct: 301 KVSTDTVPSNEKDTAYQYLWSCKDYLDKVSRIILATDSDEPGQALAEELARRLGKHRCWR 347

Query: 363 VDWPYKDDINRFKDANEI 379
           VDWPYKDD NRFKDANE+
Sbjct: 361 VDWPYKDDFNRFKDANEL 347

BLAST of Cla97C03G059780 vs. ExPASy Swiss-Prot
Match: B5X582 (Twinkle homolog protein, chloroplastic/mitochondrial OS=Arabidopsis thaliana OX=3702 GN=At1g30680 PE=1 SV=1)

HSP 1 Score: 333.6 bits (854), Expect = 3.3e-90
Identity = 181/380 (47.63%), Postives = 240/380 (63.16%), Query Frame = 0

Query: 26  PPFSHPPNDFSFSSS---PSRFLRFCTTRLASAVFNASP--SPPPGFRSFLDSVETETPL 85
           P F+  P+  S+SSS    S   RF     +  V   SP      G  S+       TP+
Sbjct: 35  PSFASYPSSPSYSSSRQVSSVSRRFRPVLASRPVSKNSPYYQRTNGLSSYNSIPRVPTPV 94

Query: 86  LFVLGFWVNVLGYPKVEVLREKMDLIGIVCDEHCTPGKYYCLFCPKCKGGRFMERSLSLH 145
              +     V+   ++  LR K+   G+   E+C PG++  L CP C+GG   E+SLSL 
Sbjct: 95  DTEVEADKRVV-LSRLVTLRRKLAEQGVDA-ENCPPGQHSGLICPTCEGGNSGEKSLSLF 154

Query: 146 VIPTGDFAMWRCFQSECGWAGRIFADGRLAFNGFSRTIKRVGWSAKESLVLEPLCDELIS 205
           + P G  A W CF+ +CG  G + ADG LA       ++R      E + LEPLCDE+  
Sbjct: 155 IAPDGSSATWNCFRGKCGLKGGVRADGGLASADPIEKVERK--ITVEGIELEPLCDEIQD 214

Query: 206 YFSKRMISQETLKRNVVMQ-IAGRQVAIAFTYRQNGQLIGCKYRTMDKRFWQEKGTEKLL 265
           YF+ R IS++TL+RN VMQ   G ++ IAFTY Q G+L+ CKYR++ K F+QE+ T ++L
Sbjct: 215 YFAARAISRKTLERNRVMQKRIGDEIVIAFTYWQRGELVSCKYRSLTKMFFQERKTRRIL 274

Query: 266 YGIDDINDADELIIVEGEMDKLSVEEAGFLNCISVPSGAPSKVSTDTVPSIEKDTAYQFL 325
           YG+DDI    E+IIVEGE+DKL++EEAGFLNC+SVP GAP+KVS+  +PS +KDT Y+FL
Sbjct: 275 YGLDDIEKTSEVIIVEGEIDKLAMEEAGFLNCVSVPDGAPAKVSSKEIPSEDKDTKYKFL 334

Query: 326 WSWKDYLDKVSRIILATDSDEPGQALAEELARRLGKHRCWRVDWPYKDDINRFKDANEIL 385
           W+  DYL K SRI++ATD D PGQA+AEE+ARRLGK RCWRV WP K +   FKDANE+L
Sbjct: 335 WNCNDYLKKASRIVIATDGDGPGQAMAEEIARRLGKERCWRVKWPKKSEDEHFKDANEVL 394

Query: 386 MHLGPDALKNAIQDAKLYQL 400
           M  GP  LK AI DA+ Y +
Sbjct: 395 MSKGPHLLKEAILDAEPYPI 410

BLAST of Cla97C03G059780 vs. ExPASy Swiss-Prot
Match: F4I6E6 (Primase homolog protein OS=Arabidopsis thaliana OX=3702 GN=At1g30660 PE=3 SV=1)

HSP 1 Score: 313.9 bits (803), Expect = 2.7e-84
Identity = 157/308 (50.97%), Postives = 207/308 (67.21%), Query Frame = 0

Query: 95  KVEVLREKMDLIGIVCDEHCTPGKYYCLFCPKCKGGRFMERSLSLHVIPTGDFAMWRCFQ 154
           K+  L  K+   GI   ++C PG   CL CPKC+ G   E+SL+L++ P G  A W C +
Sbjct: 34  KLVTLMRKLSEQGIDA-QNCPPGVRSCLICPKCEVGDSGEKSLTLYIYPDGSSAKWTC-R 93

Query: 155 SECGWAGRIFADGRLAFNGFSRTIKRVGWSAKESLVLEPLCDELISYFSKRMISQETLKR 214
            +CG  G +  DG+L        ++R      ES+ LEPLCDE+  +F+ R IS +TL+R
Sbjct: 94  RKCGLKGVLQVDGKLVSKDPIGKVERK--ITVESIKLEPLCDEIQDFFAARAISGKTLER 153

Query: 215 NVVMQ-IAGRQVAIAFTYRQNGQLIGCKYRTMDKRFWQEKGTEKLLYGIDDINDADELII 274
           N VMQ     ++ IAFTY Q G+L+ CKYR++ K+F QE+ T K+LYG+DDI +  E+II
Sbjct: 154 NRVMQKRIDDEIVIAFTYWQRGELVSCKYRSLTKKFVQERNTRKILYGLDDIEETSEIII 213

Query: 275 VEGEMDKLSVEEAGFLNCISVPSGAPSKVSTDTVPSIEKDTAYQFLWSWKDYLDKVSRII 334
           VEGE DKL++EEAGF NC+SVP GAP  VS+  +PS  KDTA++++W+  DYL K SRI+
Sbjct: 214 VEGEPDKLAMEEAGFFNCVSVPDGAPETVSSKEIPSESKDTAFKYIWNCNDYLKKASRIV 273

Query: 335 LATDSDEPGQALAEELARRLGKHRCWRVDWPYKDDINRFKDANEILMHLGPDALKNAIQD 394
           +ATD D PGQALAEELARRLGK RCW V WP K +   FKDANE+LM  GP  LK AI +
Sbjct: 274 IATDGDGPGQALAEELARRLGKERCWLVKWPKKSEDEHFKDANEVLMSKGPHLLKEAILN 333

Query: 395 AKLYQLSN 402
           A+ Y L +
Sbjct: 334 AEPYPLKS 337

BLAST of Cla97C03G059780 vs. ExPASy TrEMBL
Match: A0A0A0LYN4 (Toprim domain-containing protein OS=Cucumis sativus OX=3659 GN=Csa_1G542430 PE=4 SV=1)

HSP 1 Score: 655.6 bits (1690), Expect = 1.4e-184
Identity = 328/416 (78.85%), Postives = 349/416 (83.89%), Query Frame = 0

Query: 1   MPINSTFTFNFNFNFNFNFNFYHRSPPFSHPPNDFSFSSSPSRFLRFCTTRLASAVFNAS 60
           MPINSTFTFN NFNF F F   HRS PFSH  N+FS SSSPSRFLRFCTTRL+SAV +A 
Sbjct: 1   MPINSTFTFNSNFNFQFKF-INHRSFPFSHSLNNFSISSSPSRFLRFCTTRLSSAVSHAP 60

Query: 61  PSPPPGFRSFLDSVETETPLLFVLGFWVNVLGYPKVEVLREKMDLIGIVCDEHCTPGKYY 120
           PSPPP                              V+ L E+MDL+GIVCDEHC PGKYY
Sbjct: 61  PSPPP------------------------------VKHLMERMDLVGIVCDEHCIPGKYY 120

Query: 121 CLFCPKCKGGRFMERSLSLHVIPTGDFAMWRCFQSECGWAGRIFADGRLAFNGFSRTIKR 180
           CLFCPKCKGGR MERSLSLHVIPTGDFAMWRCFQ ECGWAG+IFADG+LAFNGF +  K 
Sbjct: 121 CLFCPKCKGGRLMERSLSLHVIPTGDFAMWRCFQFECGWAGQIFADGKLAFNGFGKITKM 180

Query: 181 VGWSAKESLVLEPLCDELISYFSKRMISQETLKRNVVMQIAGRQVAIAFTYRQNGQLIGC 240
           VG S+KESLVLEPLCDELISYFS RMISQETL+RNVVMQ+AGRQV IAFTYRQNGQL+GC
Sbjct: 181 VGRSSKESLVLEPLCDELISYFSMRMISQETLERNVVMQLAGRQVTIAFTYRQNGQLVGC 240

Query: 241 KYRTMDKRFWQEKGTEKLLYGIDDINDADELIIVEGEMDKLSVEEAGFLNCISVPSGAPS 300
           KYRTMDKRFWQEKGT K LYGIDDINDADELIIVEGEMDKLSVEEAGFLNCISVP GAP+
Sbjct: 241 KYRTMDKRFWQEKGTAKFLYGIDDINDADELIIVEGEMDKLSVEEAGFLNCISVPGGAPN 300

Query: 301 KVSTDTVPSIEKDTAYQFLWSWKDYLDKVSRIILATDSDEPGQALAEELARRLGKHRCWR 360
           KVSTDTVP IEKDTAYQ+LWS KDYLDKVSR+ILATDSD PGQALAEELARRLGK+RCWR
Sbjct: 301 KVSTDTVPPIEKDTAYQYLWSCKDYLDKVSRVILATDSDVPGQALAEELARRLGKNRCWR 360

Query: 361 VDWPYKDDINRFKDANEILMHLGPDALKNAIQDAKLYQLSNSNQIQKEEDLKSFQE 417
           V WPYKD  NRFKDANE+L+HLGPDALK AI+DAK YQLS+SNQIQKEEDL  FQE
Sbjct: 361 VSWPYKDGFNRFKDANEVLVHLGPDALKKAIEDAKFYQLSSSNQIQKEEDL--FQE 383

BLAST of Cla97C03G059780 vs. ExPASy TrEMBL
Match: A0A1S3BDI8 (primase homolog protein isoform X2 OS=Cucumis melo OX=3656 GN=LOC103488713 PE=4 SV=1)

HSP 1 Score: 652.5 bits (1682), Expect = 1.2e-183
Identity = 332/417 (79.62%), Postives = 348/417 (83.45%), Query Frame = 0

Query: 1   MPINSTFTFNFNFNFNFNFNFYHRSPPFSHPPNDFSFSSSPSRF-LRFCTTRLASAVFNA 60
           MPINSTFT  FN NFNFNF F HRS P S   N+FS SSSPSRF LRFCTTRL+SA+ +A
Sbjct: 1   MPINSTFT--FNSNFNFNFKFIHRSSPSSLSLNNFSISSSPSRFLLRFCTTRLSSALSHA 60

Query: 61  SPSPPPGFRSFLDSVETETPLLFVLGFWVNVLGYPKVEVLREKMDLIGIVCDEHCTPGKY 120
            P PPP                              V+VLREKMDLIGIVCDEHC PGKY
Sbjct: 61  PPLPPP------------------------------VKVLREKMDLIGIVCDEHCIPGKY 120

Query: 121 YCLFCPKCKGGRFMERSLSLHVIPTGDFAMWRCFQSECGWAGRIFADGRLAFNGFSRTIK 180
           YCLFCPKCKGGR MERSLSLHVIPTGDFAMWRCF+ ECGWAGRIFADGRLAFNGFS+T K
Sbjct: 121 YCLFCPKCKGGRLMERSLSLHVIPTGDFAMWRCFRFECGWAGRIFADGRLAFNGFSKTEK 180

Query: 181 RVGWSAKESLVLEPLCDELISYFSKRMISQETLKRNVVMQIAGRQVAIAFTYRQNGQLIG 240
            VG S+KESLVLEPLCDELISYFS RMISQETL+RNVVMQ+ GRQVAIAFTYRQNGQL+G
Sbjct: 181 MVGRSSKESLVLEPLCDELISYFSMRMISQETLERNVVMQVVGRQVAIAFTYRQNGQLVG 240

Query: 241 CKYRTMDKRFWQEKGTEKLLYGIDDINDADELIIVEGEMDKLSVEEAGFLNCISVPSGAP 300
           CKYRTMDKRFWQEKGT K LYGIDDINDADELIIVEGEMDKLSVEEAGFLNCISVP GAP
Sbjct: 241 CKYRTMDKRFWQEKGTAKFLYGIDDINDADELIIVEGEMDKLSVEEAGFLNCISVPGGAP 300

Query: 301 SKVSTDTVPSIEKDTAYQFLWSWKDYLDKVSRIILATDSDEPGQALAEELARRLGKHRCW 360
           SKVSTDT+P IEKD+AYQ+LW  KDYLDKVSRIILATDSD PGQALAEELARRLGKHRCW
Sbjct: 301 SKVSTDTIPPIEKDSAYQYLWGCKDYLDKVSRIILATDSDVPGQALAEELARRLGKHRCW 360

Query: 361 RVDWPYKDDINRFKDANEILMHLGPDALKNAIQDAKLYQLSNSNQIQKEEDLKSFQE 417
           RVDWPYKD  NRFKDANE+L+HLGPDALKNAIQDAK YQLS S  IQKEE L  FQE
Sbjct: 361 RVDWPYKDGFNRFKDANEVLVHLGPDALKNAIQDAKFYQLSISISIQKEEGL--FQE 383

BLAST of Cla97C03G059780 vs. ExPASy TrEMBL
Match: A0A1S4DTX2 (primase homolog protein isoform X1 OS=Cucumis melo OX=3656 GN=LOC103488713 PE=4 SV=1)

HSP 1 Score: 647.9 bits (1670), Expect = 2.9e-182
Identity = 332/418 (79.43%), Postives = 348/418 (83.25%), Query Frame = 0

Query: 1   MPINSTFTFNFNFNFNFNFNFYHRSPPFSHPPNDFSFSSSPSRF-LRFCTTRLASAVFNA 60
           MPINSTFT  FN NFNFNF F HRS P S   N+FS SSSPSRF LRFCTTRL+SA+ +A
Sbjct: 1   MPINSTFT--FNSNFNFNFKFIHRSSPSSLSLNNFSISSSPSRFLLRFCTTRLSSALSHA 60

Query: 61  SPSPPPGFRSFLDSVETETPLLFVLGFWVNVLGYPKVEVLREKMDLIGIVCDEHCTPGKY 120
            P PPP                              V+VLREKMDLIGIVCDEHC PGKY
Sbjct: 61  PPLPPP------------------------------VKVLREKMDLIGIVCDEHCIPGKY 120

Query: 121 YCLFCPKCKGGRFMERSLSLHVIPTGDFAMWRCFQSECGWAGRIFADGRLAFNGFSRTIK 180
           YCLFCPKCKGGR MERSLSLHVIPTGDFAMWRCF+ ECGWAGRIFADGRLAFNGFS+T K
Sbjct: 121 YCLFCPKCKGGRLMERSLSLHVIPTGDFAMWRCFRFECGWAGRIFADGRLAFNGFSKTEK 180

Query: 181 RVGWSAKESLVLEPLCDELISYFSKRMISQETLKRNVVMQIAGR-QVAIAFTYRQNGQLI 240
            VG S+KESLVLEPLCDELISYFS RMISQETL+RNVVMQ+ GR QVAIAFTYRQNGQL+
Sbjct: 181 MVGRSSKESLVLEPLCDELISYFSMRMISQETLERNVVMQVVGRQQVAIAFTYRQNGQLV 240

Query: 241 GCKYRTMDKRFWQEKGTEKLLYGIDDINDADELIIVEGEMDKLSVEEAGFLNCISVPSGA 300
           GCKYRTMDKRFWQEKGT K LYGIDDINDADELIIVEGEMDKLSVEEAGFLNCISVP GA
Sbjct: 241 GCKYRTMDKRFWQEKGTAKFLYGIDDINDADELIIVEGEMDKLSVEEAGFLNCISVPGGA 300

Query: 301 PSKVSTDTVPSIEKDTAYQFLWSWKDYLDKVSRIILATDSDEPGQALAEELARRLGKHRC 360
           PSKVSTDT+P IEKD+AYQ+LW  KDYLDKVSRIILATDSD PGQALAEELARRLGKHRC
Sbjct: 301 PSKVSTDTIPPIEKDSAYQYLWGCKDYLDKVSRIILATDSDVPGQALAEELARRLGKHRC 360

Query: 361 WRVDWPYKDDINRFKDANEILMHLGPDALKNAIQDAKLYQLSNSNQIQKEEDLKSFQE 417
           WRVDWPYKD  NRFKDANE+L+HLGPDALKNAIQDAK YQLS S  IQKEE L  FQE
Sbjct: 361 WRVDWPYKDGFNRFKDANEVLVHLGPDALKNAIQDAKFYQLSISISIQKEEGL--FQE 384

BLAST of Cla97C03G059780 vs. ExPASy TrEMBL
Match: A0A1S4DT34 (primase homolog protein isoform X3 OS=Cucumis melo OX=3656 GN=LOC103488713 PE=4 SV=1)

HSP 1 Score: 609.8 bits (1571), Expect = 8.7e-171
Identity = 320/418 (76.56%), Postives = 334/418 (79.90%), Query Frame = 0

Query: 1   MPINSTFTFNFNFNFNFNFNFYHRSPPFSHPPNDFSFSSSPSRF-LRFCTTRLASAVFNA 60
           MPINSTFT  FN NFNFNF F HRS P S   N+FS SSSPSRF LRFCTTRL+SA+ +A
Sbjct: 1   MPINSTFT--FNSNFNFNFKFIHRSSPSSLSLNNFSISSSPSRFLLRFCTTRLSSALSHA 60

Query: 61  SPSPPPGFRSFLDSVETETPLLFVLGFWVNVLGYPKVEVLREKMDLIGIVCDEHCTPGKY 120
            P PPP                              V+VLREKMDLIGIVCDEHC PGKY
Sbjct: 61  PPLPPP------------------------------VKVLREKMDLIGIVCDEHCIPGKY 120

Query: 121 YCLFCPKCKGGRFMERSLSLHVIPTGDFAMWRCFQSECGWAGRIFADGRLAFNGFSRTIK 180
           YCLFCPKCKGGR MERSLSLHVIPTGDFAMWRCF+ ECGWAGRIFADGRLAFNGFS+T K
Sbjct: 121 YCLFCPKCKGGRLMERSLSLHVIPTGDFAMWRCFRFECGWAGRIFADGRLAFNGFSKTEK 180

Query: 181 RVGWSAKESLVLEPLCDELISYFSKRMISQETLKRNVVMQIAGR-QVAIAFTYRQNGQLI 240
            VG S+KESLVLEPLCDELISYFS RMISQETL+RNVVMQ+ GR QVAIAFTYRQNGQL+
Sbjct: 181 MVGRSSKESLVLEPLCDELISYFSMRMISQETLERNVVMQVVGRQQVAIAFTYRQNGQLV 240

Query: 241 GCKYRTMDKRFWQEKGTEKLLYGIDDINDADELIIVEGEMDKLSVEEAGFLNCISVPSGA 300
           GCKYRTMDKRFWQEKGT K LYGIDDINDADELIIVEGEMDKLSVEEAGFLNCISVP GA
Sbjct: 241 GCKYRTMDKRFWQEKGTAKFLYGIDDINDADELIIVEGEMDKLSVEEAGFLNCISVPGGA 300

Query: 301 PSKVSTDTVPSIEKDTAYQFLWSWKDYLDKVSRIILATDSDEPGQALAEELARRLGKHRC 360
           PSKVSTDT+P IE                KVSRIILATDSD PGQALAEELARRLGKHRC
Sbjct: 301 PSKVSTDTIPPIE----------------KVSRIILATDSDVPGQALAEELARRLGKHRC 360

Query: 361 WRVDWPYKDDINRFKDANEILMHLGPDALKNAIQDAKLYQLSNSNQIQKEEDLKSFQE 417
           WRVDWPYKD  NRFKDANE+L+HLGPDALKNAIQDAK YQLS S  IQKEE L  FQE
Sbjct: 361 WRVDWPYKDGFNRFKDANEVLVHLGPDALKNAIQDAKFYQLSISISIQKEEGL--FQE 368

BLAST of Cla97C03G059780 vs. ExPASy TrEMBL
Match: A0A1S4DT27 (primase homolog protein isoform X4 OS=Cucumis melo OX=3656 GN=LOC103488713 PE=4 SV=1)

HSP 1 Score: 598.2 bits (1541), Expect = 2.6e-167
Identity = 302/379 (79.68%), Postives = 316/379 (83.38%), Query Frame = 0

Query: 1   MPINSTFTFNFNFNFNFNFNFYHRSPPFSHPPNDFSFSSSPSRF-LRFCTTRLASAVFNA 60
           MPINSTFT  FN NFNFNF F HRS P S   N+FS SSSPSRF LRFCTTRL+SA+ +A
Sbjct: 1   MPINSTFT--FNSNFNFNFKFIHRSSPSSLSLNNFSISSSPSRFLLRFCTTRLSSALSHA 60

Query: 61  SPSPPPGFRSFLDSVETETPLLFVLGFWVNVLGYPKVEVLREKMDLIGIVCDEHCTPGKY 120
            P PPP                              V+VLREKMDLIGIVCDEHC PGKY
Sbjct: 61  PPLPPP------------------------------VKVLREKMDLIGIVCDEHCIPGKY 120

Query: 121 YCLFCPKCKGGRFMERSLSLHVIPTGDFAMWRCFQSECGWAGRIFADGRLAFNGFSRTIK 180
           YCLFCPKCKGGR MERSLSLHVIPTGDFAMWRCF+ ECGWAGRIFADGRLAFNGFS+T K
Sbjct: 121 YCLFCPKCKGGRLMERSLSLHVIPTGDFAMWRCFRFECGWAGRIFADGRLAFNGFSKTEK 180

Query: 181 RVGWSAKESLVLEPLCDELISYFSKRMISQETLKRNVVMQIAGR-QVAIAFTYRQNGQLI 240
            VG S+KESLVLEPLCDELISYFS RMISQETL+RNVVMQ+ GR QVAIAFTYRQNGQL+
Sbjct: 181 MVGRSSKESLVLEPLCDELISYFSMRMISQETLERNVVMQVVGRQQVAIAFTYRQNGQLV 240

Query: 241 GCKYRTMDKRFWQEKGTEKLLYGIDDINDADELIIVEGEMDKLSVEEAGFLNCISVPSGA 300
           GCKYRTMDKRFWQEKGT K LYGIDDINDADELIIVEGEMDKLSVEEAGFLNCISVP GA
Sbjct: 241 GCKYRTMDKRFWQEKGTAKFLYGIDDINDADELIIVEGEMDKLSVEEAGFLNCISVPGGA 300

Query: 301 PSKVSTDTVPSIEKDTAYQFLWSWKDYLDKVSRIILATDSDEPGQALAEELARRLGKHRC 360
           PSKVSTDT+P IEKD+AYQ+LW  KDYLDKVSRIILATDSD PGQALAEELARRLGKHRC
Sbjct: 301 PSKVSTDTIPPIEKDSAYQYLWGCKDYLDKVSRIILATDSDVPGQALAEELARRLGKHRC 347

Query: 361 WRVDWPYKDDINRFKDANE 378
           WRVDWPYKD  NRFKDANE
Sbjct: 361 WRVDWPYKDGFNRFKDANE 347

BLAST of Cla97C03G059780 vs. TAIR 10
Match: AT1G30680.1 (toprim domain-containing protein )

HSP 1 Score: 333.6 bits (854), Expect = 2.3e-91
Identity = 181/380 (47.63%), Postives = 240/380 (63.16%), Query Frame = 0

Query: 26  PPFSHPPNDFSFSSS---PSRFLRFCTTRLASAVFNASP--SPPPGFRSFLDSVETETPL 85
           P F+  P+  S+SSS    S   RF     +  V   SP      G  S+       TP+
Sbjct: 35  PSFASYPSSPSYSSSRQVSSVSRRFRPVLASRPVSKNSPYYQRTNGLSSYNSIPRVPTPV 94

Query: 86  LFVLGFWVNVLGYPKVEVLREKMDLIGIVCDEHCTPGKYYCLFCPKCKGGRFMERSLSLH 145
              +     V+   ++  LR K+   G+   E+C PG++  L CP C+GG   E+SLSL 
Sbjct: 95  DTEVEADKRVV-LSRLVTLRRKLAEQGVDA-ENCPPGQHSGLICPTCEGGNSGEKSLSLF 154

Query: 146 VIPTGDFAMWRCFQSECGWAGRIFADGRLAFNGFSRTIKRVGWSAKESLVLEPLCDELIS 205
           + P G  A W CF+ +CG  G + ADG LA       ++R      E + LEPLCDE+  
Sbjct: 155 IAPDGSSATWNCFRGKCGLKGGVRADGGLASADPIEKVERK--ITVEGIELEPLCDEIQD 214

Query: 206 YFSKRMISQETLKRNVVMQ-IAGRQVAIAFTYRQNGQLIGCKYRTMDKRFWQEKGTEKLL 265
           YF+ R IS++TL+RN VMQ   G ++ IAFTY Q G+L+ CKYR++ K F+QE+ T ++L
Sbjct: 215 YFAARAISRKTLERNRVMQKRIGDEIVIAFTYWQRGELVSCKYRSLTKMFFQERKTRRIL 274

Query: 266 YGIDDINDADELIIVEGEMDKLSVEEAGFLNCISVPSGAPSKVSTDTVPSIEKDTAYQFL 325
           YG+DDI    E+IIVEGE+DKL++EEAGFLNC+SVP GAP+KVS+  +PS +KDT Y+FL
Sbjct: 275 YGLDDIEKTSEVIIVEGEIDKLAMEEAGFLNCVSVPDGAPAKVSSKEIPSEDKDTKYKFL 334

Query: 326 WSWKDYLDKVSRIILATDSDEPGQALAEELARRLGKHRCWRVDWPYKDDINRFKDANEIL 385
           W+  DYL K SRI++ATD D PGQA+AEE+ARRLGK RCWRV WP K +   FKDANE+L
Sbjct: 335 WNCNDYLKKASRIVIATDGDGPGQAMAEEIARRLGKERCWRVKWPKKSEDEHFKDANEVL 394

Query: 386 MHLGPDALKNAIQDAKLYQL 400
           M  GP  LK AI DA+ Y +
Sbjct: 395 MSKGPHLLKEAILDAEPYPI 410

BLAST of Cla97C03G059780 vs. TAIR 10
Match: AT1G30660.1 (nucleic acid binding;nucleic acid binding )

HSP 1 Score: 313.9 bits (803), Expect = 1.9e-85
Identity = 157/308 (50.97%), Postives = 207/308 (67.21%), Query Frame = 0

Query: 95  KVEVLREKMDLIGIVCDEHCTPGKYYCLFCPKCKGGRFMERSLSLHVIPTGDFAMWRCFQ 154
           K+  L  K+   GI   ++C PG   CL CPKC+ G   E+SL+L++ P G  A W C +
Sbjct: 34  KLVTLMRKLSEQGIDA-QNCPPGVRSCLICPKCEVGDSGEKSLTLYIYPDGSSAKWTC-R 93

Query: 155 SECGWAGRIFADGRLAFNGFSRTIKRVGWSAKESLVLEPLCDELISYFSKRMISQETLKR 214
            +CG  G +  DG+L        ++R      ES+ LEPLCDE+  +F+ R IS +TL+R
Sbjct: 94  RKCGLKGVLQVDGKLVSKDPIGKVERK--ITVESIKLEPLCDEIQDFFAARAISGKTLER 153

Query: 215 NVVMQ-IAGRQVAIAFTYRQNGQLIGCKYRTMDKRFWQEKGTEKLLYGIDDINDADELII 274
           N VMQ     ++ IAFTY Q G+L+ CKYR++ K+F QE+ T K+LYG+DDI +  E+II
Sbjct: 154 NRVMQKRIDDEIVIAFTYWQRGELVSCKYRSLTKKFVQERNTRKILYGLDDIEETSEIII 213

Query: 275 VEGEMDKLSVEEAGFLNCISVPSGAPSKVSTDTVPSIEKDTAYQFLWSWKDYLDKVSRII 334
           VEGE DKL++EEAGF NC+SVP GAP  VS+  +PS  KDTA++++W+  DYL K SRI+
Sbjct: 214 VEGEPDKLAMEEAGFFNCVSVPDGAPETVSSKEIPSESKDTAFKYIWNCNDYLKKASRIV 273

Query: 335 LATDSDEPGQALAEELARRLGKHRCWRVDWPYKDDINRFKDANEILMHLGPDALKNAIQD 394
           +ATD D PGQALAEELARRLGK RCW V WP K +   FKDANE+LM  GP  LK AI +
Sbjct: 274 IATDGDGPGQALAEELARRLGKERCWLVKWPKKSEDEHFKDANEVLMSKGPHLLKEAILN 333

Query: 395 AKLYQLSN 402
           A+ Y L +
Sbjct: 334 AEPYPLKS 337

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
XP_038893950.14.0e-19483.17primase homolog protein isoform X1 [Benincasa hispida][more]
XP_011659247.12.9e-18478.85primase homolog protein isoform X1 [Cucumis sativus] >KGN65952.1 hypothetical pr... [more]
XP_008445790.12.4e-18379.62PREDICTED: primase homolog protein isoform X2 [Cucumis melo][more]
XP_016899150.16.0e-18279.43PREDICTED: primase homolog protein isoform X1 [Cucumis melo][more]
XP_038893951.15.4e-17582.54primase homolog protein isoform X2 [Benincasa hispida][more]
Match NameE-valueIdentityDescription
B5X5823.3e-9047.63Twinkle homolog protein, chloroplastic/mitochondrial OS=Arabidopsis thaliana OX=... [more]
F4I6E62.7e-8450.97Primase homolog protein OS=Arabidopsis thaliana OX=3702 GN=At1g30660 PE=3 SV=1[more]
Match NameE-valueIdentityDescription
A0A0A0LYN41.4e-18478.85Toprim domain-containing protein OS=Cucumis sativus OX=3659 GN=Csa_1G542430 PE=4... [more]
A0A1S3BDI81.2e-18379.62primase homolog protein isoform X2 OS=Cucumis melo OX=3656 GN=LOC103488713 PE=4 ... [more]
A0A1S4DTX22.9e-18279.43primase homolog protein isoform X1 OS=Cucumis melo OX=3656 GN=LOC103488713 PE=4 ... [more]
A0A1S4DT348.7e-17176.56primase homolog protein isoform X3 OS=Cucumis melo OX=3656 GN=LOC103488713 PE=4 ... [more]
A0A1S4DT272.6e-16779.68primase homolog protein isoform X4 OS=Cucumis melo OX=3656 GN=LOC103488713 PE=4 ... [more]
Match NameE-valueIdentityDescription
AT1G30680.12.3e-9147.63toprim domain-containing protein [more]
AT1G30660.11.9e-8550.97nucleic acid binding;nucleic acid binding [more]
InterPro
Analysis Name: InterPro Annotations of Watermelon (97103) v2.5
Date Performed: 2022-01-31
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR006171TOPRIM domainSMARTSM00493toprim5coord: 269..358
e-value: 4.4E-8
score: 42.9
IPR006171TOPRIM domainPFAMPF13662Toprim_4coord: 269..361
e-value: 7.1E-19
score: 68.1
NoneNo IPR availableGENE3D3.40.1360.10coord: 256..398
e-value: 2.6E-33
score: 116.7
NoneNo IPR availablePANTHERPTHR12873:SF1ACID-BINDING PROTEIN, PUTATIVE-RELATEDcoord: 93..398
NoneNo IPR availableSUPERFAMILY56731DNA primase corecoord: 201..377
IPR027032Twinkle-like proteinPANTHERPTHR12873T7-LIKE MITOCHONDRIAL DNA HELICASEcoord: 93..398
IPR034154Archaeal primase DnaG/twinkle, TOPRIM domainCDDcd01029TOPRIM_primasescoord: 269..359
e-value: 5.22464E-11
score: 56.5098

Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
Cla97C03G059780.1Cla97C03G059780.1mRNA


GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
biological_process GO:0032508 DNA duplex unwinding
molecular_function GO:0043139 5'-3' DNA helicase activity
molecular_function GO:0003697 single-stranded DNA binding