Cla97C03G057680 (gene) Watermelon (97103) v2.5

Overview


Name	Cla97C03G057680
Type	gene
Organism	Citrullus lanatus subsp. vulgaris cv. 97103 (Watermelon (97103) v2.5)
Description	glucan endo-1,3-beta-glucosidase 13
Location	Cla97Chr03: 6647786 .. 6649233 (-)
RNA-Seq Expression	Cla97C03G057680
Synteny	Cla97C03G057680

Sequences

The following sequences are available for this feature:

Gene sequence (with intron)

Legend: polypeptideexonCDS

Hold the cursor over a type above to highlight its positions in the sequence below.

ATGAGAAGAAAAATGTGGGTTTTGGAGATCATCATCCTCCTGCTACATTATTTCTTTGTGGGTATGTTTTGATTTTATGGCATTTTAAGAAAGCAGGAAAAAACAGAGTCCTGTGTTTTGTTTTGCATATTGAGTGAAAGTGTTGTGGTTTTGTTGTTAGGGGGAGCGACGGCGGCGGAGGCAGGAATGCCGGTGACGGCGGGATGGACGCCGCCGGAAGGGAACACGACGTTTATAGAAGGAACAACGTGGTGCGTGGCAAAGAGTGGAGTTTCGCAGATTGATCTGCAGAATGCTTTGGATTGGGCTTGTGGATTGGGAAAAGCAGATTGCAAAGCCATTCAAAAGGGAGGGCCTTGTTATCAACCCGATACTCTTTTGTCTCATGCTTCTTATGCATTTAATAGCTATTATCAGCAAAATGGCAACTCTGATATTGCTTGCAACTTTGGTGGCACTGCTACTTTGACCAAGAAAAACCCAAGTAAAATCTCTCTCTCTCCTTGCTTTGGTTTTTATATTTCTCTATAATGTTCTTACCCAATTTCCAAAAAAAAAAAAAAAAAACAAAAAAATTAACTAAATATATATGTTTATAGGTTTAATTTTCAAAAGCAAAAACCAAATCGTTATCAAATAAATGTTTTTTTTCTTTATATTTTATGAATATTTATAGAAGATATTGGAGATATTGATATTTGATGTCGAACCTTCTGATTTGTCGATATATTAATTCATCGAGTAATTTTTTTATCCTTGGCTTACATTGACTATATATAGTTGAGTTAAAGTTATTATTTTGGATGGGTATGGTTTGAAGTTGGATTTTTGATTTGTGTGAATTGAATGTGGGGTTTTTTTTTTTTCTTCTTTCCTCTTCTAAGAATGTTTTATGGTGGGTGAGGTTAGAAGATTCGAATCTTAGATTTTTGTAGAGGGAGTTAGAGATGTCTTTGTTAGTAAGTTATGCTTTGAGTTGAAAACTTAGAAAAGGTAATGAGTAGATCGTTCTATTTATTGTAATCAATGGATGAGACGAAAATAATATTTATAAGTTTAATTTTCATAAATCAAATAGTTATGAAATAGATGGTAGGAAAAGGAATGTTTTCTCAATCTTAAAAACAATTCCATCTTAAATGTTATAAACCATTCCTCATTGCAGCTTCTTTTTTTATTATTATTTTTACAGGTTATGGGAAATGTGACTATTCAGCATCCAGGTATTTTTTTTTTTTTTTCACATGAAATTTGACAATAATTTTTTTTAAAACAATCACTAAATATTAACCATAAAATAATATTGAAGCTTTTTTTTTTTTTGGAAAATGGCCCTCAGTTCAACAAGCTCAGCACCTTCAAAATCTAAGAACAGTTCAAGCCAAATCTGCATGAAGTTGCTTTCTGGGGTTTTTATGCTTCTAAATTTATATTTTGGAAGCTCCTAA

mRNA sequence

ATGAGAAGAAAAATGTGGGTTTTGGAGATCATCATCCTCCTGCTACATTATTTCTTTGTGGGGGGAGCGACGGCGGCGGAGGCAGGAATGCCGGTGACGGCGGGATGGACGCCGCCGGAAGGGAACACGACGTTTATAGAAGGAACAACGTGGTGCGTGGCAAAGAGTGGAGTTTCGCAGATTGATCTGCAGAATGCTTTGGATTGGGCTTGTGGATTGGGAAAAGCAGATTGCAAAGCCATTCAAAAGGGAGGGCCTTGTTATCAACCCGATACTCTTTTGTCTCATGCTTCTTATGCATTTAATAGCTATTATCAGCAAAATGGCAACTCTGATATTGCTTGCAACTTTGGTGGCACTGCTACTTTGACCAAGAAAAACCCAAGTTATGGGAAATGTGACTATTCAGCATCCAGTTCAACAAGCTCAGCACCTTCAAAATCTAAGAACAGTTCAAGCCAAATCTGCATGAAGTTGCTTTCTGGGGTTTTTATGCTTCTAAATTTATATTTTGGAAGCTCCTAA

Coding sequence (CDS)

ATGAGAAGAAAAATGTGGGTTTTGGAGATCATCATCCTCCTGCTACATTATTTCTTTGTGGGGGGAGCGACGGCGGCGGAGGCAGGAATGCCGGTGACGGCGGGATGGACGCCGCCGGAAGGGAACACGACGTTTATAGAAGGAACAACGTGGTGCGTGGCAAAGAGTGGAGTTTCGCAGATTGATCTGCAGAATGCTTTGGATTGGGCTTGTGGATTGGGAAAAGCAGATTGCAAAGCCATTCAAAAGGGAGGGCCTTGTTATCAACCCGATACTCTTTTGTCTCATGCTTCTTATGCATTTAATAGCTATTATCAGCAAAATGGCAACTCTGATATTGCTTGCAACTTTGGTGGCACTGCTACTTTGACCAAGAAAAACCCAAGTTATGGGAAATGTGACTATTCAGCATCCAGTTCAACAAGCTCAGCACCTTCAAAATCTAAGAACAGTTCAAGCCAAATCTGCATGAAGTTGCTTTCTGGGGTTTTTATGCTTCTAAATTTATATTTTGGAAGCTCCTAA

Protein sequence

MRRKMWVLEIIILLLHYFFVGGATAAEAGMPVTAGWTPPEGNTTFIEGTTWCVAKSGVSQIDLQNALDWACGLGKADCKAIQKGGPCYQPDTLLSHASYAFNSYYQQNGNSDIACNFGGTATLTKKNPSYGKCDYSASSSTSSAPSKSKNSSSQICMKLLSGVFMLLNLYFGSS

Homology

[Close]

BLAST of Cla97C03G057680 vs. NCBI nr
Match: XP_038895270.1 (major pollen allergen Ole e 10 [Benincasa hispida])

HSP 1 Score: 303.1 bits (775), Expect = 1.5e-78
Identity = 151/175 (86.29%), Postives = 161/175 (92.00%), Query Frame = 0


Query: 1   MRRKMWVLEIIILLLHYFFVGGATAAEAGMPV-TAGWTPPEGNTTFIEGTTWCVAKSGVS 60
           M+RK+WVLE I+LLLHY F+GG TAAEAGM V TA  TPPEGNTTF++GT+WCVAK+G S
Sbjct: 1   MKRKIWVLE-ILLLLHYLFLGGGTAAEAGMAVTTAAMTPPEGNTTFLDGTSWCVAKAGAS 60

Query: 61  QIDLQNALDWACGLGKADCKAIQKGGPCYQPDTLLSHASYAFNSYYQQNGNSDIACNFGG 120
           QIDLQNALDWACGLGKADC+AIQKGGPCYQPDTLLSHASYAFNSYYQQNGNSDIACNFGG
Sbjct: 61  QIDLQNALDWACGLGKADCRAIQKGGPCYQPDTLLSHASYAFNSYYQQNGNSDIACNFGG 120

Query: 121 TATLTKKNPSYGKCDYSASSSTSSAPSKSKNSSSQICMKLLSGVFMLLNLYFGSS 175
            ATLTK NPSYGKCDYSASSSTSSAPSK KNS S IC+KLL GVFMLLNLYFGSS
Sbjct: 121 CATLTKNNPSYGKCDYSASSSTSSAPSKFKNSPSHICLKLLCGVFMLLNLYFGSS 174

[Close]

BLAST of Cla97C03G057680 vs. NCBI nr
Match: XP_008444815.1 (PREDICTED: glucan endo-1,3-beta-glucosidase 13 [Cucumis melo])

HSP 1 Score: 293.1 bits (749), Expect = 1.6e-75
Identity = 146/173 (84.39%), Postives = 159/173 (91.91%), Query Frame = 0


Query: 1   MRRKMWVLEIIILLLHYFFVGGATAAEAGMPVTAGWTPPEGNTTFIEGTTWCVAKSGVSQ 60
           M+ K+WVLE I+L LHYFF+GGA AAEAGMPV A   P EGNTTFI+GTTWCVAK GVSQ
Sbjct: 1   MKTKIWVLE-ILLFLHYFFLGGAMAAEAGMPVMA--APVEGNTTFIDGTTWCVAKPGVSQ 60

Query: 61  IDLQNALDWACGLGKADCKAIQKGGPCYQPDTLLSHASYAFNSYYQQNGNSDIACNFGGT 120
           IDLQNALDWACG+GKADC+AIQKGG CY+PDTLLSHASYAFNSYYQQNGNSDIACNFGG 
Sbjct: 61  IDLQNALDWACGMGKADCRAIQKGGRCYEPDTLLSHASYAFNSYYQQNGNSDIACNFGGC 120

Query: 121 ATLTKKNPSYGKCDYSASSSTSSAPSKSKNSSSQICMKLLSGVFMLLNLYFGS 174
           ATLTKK+PSYGKCDYSASSST+SAPSKSKNS S IC+KLLSGVF++LNLYFGS
Sbjct: 121 ATLTKKDPSYGKCDYSASSSTNSAPSKSKNSPSHICLKLLSGVFVVLNLYFGS 170

[Close]

BLAST of Cla97C03G057680 vs. NCBI nr
Match: XP_004135538.1 (major pollen allergen Ole e 10 [Cucumis sativus] >KAE8653300.1 hypothetical protein Csa_023370 [Cucumis sativus])

HSP 1 Score: 290.4 bits (742), Expect = 1.0e-74
Identity = 142/174 (81.61%), Postives = 160/174 (91.95%), Query Frame = 0


Query: 1   MRRKMWVLEIIILLLHYFFVGGATAAEAGMPVTAGWTPPEGNTTFIEGTTWCVAKSGVSQ 60
           M+ K+WVLEI++L LHYFF+GGA AAEAGMPV A   P EGNTTFI+GTTWCVAK+GVSQ
Sbjct: 1   MKTKIWVLEILLLFLHYFFLGGAMAAEAGMPVMA--APAEGNTTFIDGTTWCVAKAGVSQ 60

Query: 61  IDLQNALDWACGLGKADCKAIQKGGPCYQPDTLLSHASYAFNSYYQQNGNSDIACNFGGT 120
           IDLQNALDWACG+GKADC+AIQKGG CY+PDTLLSHAS+AFNSYYQQNGNSDIACNFGG 
Sbjct: 61  IDLQNALDWACGMGKADCRAIQKGGRCYEPDTLLSHASFAFNSYYQQNGNSDIACNFGGC 120

Query: 121 ATLTKKNPSYGKCDYSASSSTSSAPSKSKNSSSQICMKLLSGVFMLLNLYFGSS 175
           ATLTKK+PSYGKCDYSASSST+SAPSKSKNS + IC+KL+S V ++LNLYFGSS
Sbjct: 121 ATLTKKDPSYGKCDYSASSSTNSAPSKSKNSPTHICLKLVSVVSVVLNLYFGSS 172

[Close]

BLAST of Cla97C03G057680 vs. NCBI nr
Match: XP_022140209.1 (major pollen allergen Ole e 10 [Momordica charantia])

HSP 1 Score: 252.7 bits (644), Expect = 2.3e-63
Identity = 126/180 (70.00%), Postives = 149/180 (82.78%), Query Frame = 0


Query: 1   MRRKMWVLEIII---LLLHYFFVGGATA----AEAGMPVTAGWTPPEGNTTFIEGTTWCV 60
           MR K+WVL +++    LL   F+G   A    AEA MP T   +PPEGN TF++GT+WCV
Sbjct: 1   MRTKIWVLLLVLEAMFLLQCCFLGEGMAVEEKAEAAMPATT-LSPPEGNATFLDGTSWCV 60

Query: 61  AKSGVSQIDLQNALDWACGLGKADCKAIQKGGPCYQPDTLLSHASYAFNSYYQQNGNSDI 120
           A++G SQIDLQNALDWACGLGKADC+AIQKGG CYQPDTLLSHASYAFNS+YQQNGNSDI
Sbjct: 61  ARAGASQIDLQNALDWACGLGKADCRAIQKGGACYQPDTLLSHASYAFNSFYQQNGNSDI 120

Query: 121 ACNFGGTATLTKKNPSYGKCDYSASSSTSSAPSKSKNSSSQICMKLLSGVFMLLNLYFGS 174
           ACNFGG+ATLTKKNPSYGKCDYSASSS +SA SK KN+ S +C++L++G+ M+LNLY GS
Sbjct: 121 ACNFGGSATLTKKNPSYGKCDYSASSSVNSATSKLKNNGSHVCLRLVAGILMVLNLYLGS 179

[Close]

BLAST of Cla97C03G057680 vs. NCBI nr
Match: XP_022994908.1 (major pollen allergen Ole e 10-like [Cucurbita maxima])

HSP 1 Score: 251.1 bits (640), Expect = 6.8e-63
Identity = 126/180 (70.00%), Postives = 149/180 (82.78%), Query Frame = 0


Query: 1   MRRKMWVLEIIILLLHYFFVGGATA-------AEAGMPVTAGWTPPEGNTTFIEGTTWCV 60
           M  ++W L + + LL  F +G A A       AEA MP+T  W+PPEGNTTF++GT+WCV
Sbjct: 1   MSIQIWGL-VALFLLQSFLMGEAMATAVAVEKAEASMPMTT-WSPPEGNTTFLDGTSWCV 60

Query: 61  AKSGVSQIDLQNALDWACGLGKADCKAIQKGGPCYQPDTLLSHASYAFNSYYQQNGNSDI 120
           AK GVSQIDLQNALDWACGLGKADC+AIQKGG CYQPDTL SHASYAFNSYYQQNGNSDI
Sbjct: 61  AKPGVSQIDLQNALDWACGLGKADCRAIQKGGRCYQPDTLFSHASYAFNSYYQQNGNSDI 120

Query: 121 ACNFGGTATLTKKNPSYGKCDYSASSSTSSAPSKSKNSSSQICMKLLSGVFMLLNLYFGS 174
           ACNFGG+ATLTK++PSYGKCDYSAS S SSAPSK K + S+ C++L+ G++M++NLYFGS
Sbjct: 121 ACNFGGSATLTKRDPSYGKCDYSASRSASSAPSKWKKNGSRGCLQLVGGIWMVMNLYFGS 178

[Close]

BLAST of Cla97C03G057680 vs. ExPASy Swiss-Prot
Match: Q9FJU9 (Glucan endo-1,3-beta-glucosidase 13 OS=Arabidopsis thaliana OX=3702 GN=At5g56590 PE=2 SV=1)

HSP 1 Score: 119.4 bits (298), Expect = 4.1e-26
Identity = 63/142 (44.37%), Postives = 86/142 (60.56%), Query Frame = 0


Query: 41  GNTTFIEGTTWCVAKSGVSQIDLQNALDWACGLGKADCKAIQKGGPCYQPDTLLSHASYA 100
           G  +     +WC+A S  S+ DL+ ALDWACG G  DC AIQ   PC+QPDTL+SHAS+ 
Sbjct: 359 GTNSSGSSNSWCIASSKASERDLKGALDWACGPGNVDCTAIQPSQPCFQPDTLVSHASFV 418

Query: 101 FNSYYQQNGNSDIACNFGGTATLTKKNPSYGKCDY-----------SASSSTSSAPSKSK 160
           FNSY+QQN  +D+AC+FGG      K+PSY KC Y           +A++ TSSA +   
Sbjct: 419 FNSYFQQNRATDVACSFGGAGVKVNKDPSYDKCIYITAGGNKTKATNATALTSSASTPRG 478

Query: 161 NSSSQICMKLLSGVFMLLNLYF 172
           N   Q  +KL     ++++L+F
Sbjct: 479 NELLQWILKLC----LMISLFF 496

[Close]

BLAST of Cla97C03G057680 vs. ExPASy Swiss-Prot
Match: Q8VYE5 (Glucan endo-1,3-beta-glucosidase 12 OS=Arabidopsis thaliana OX=3702 GN=At4g29360 PE=2 SV=1)

HSP 1 Score: 117.9 bits (294), Expect = 1.2e-25
Identity = 59/137 (43.07%), Postives = 77/137 (56.20%), Query Frame = 0


Query: 19  FVGGATAAEAGMPVTAGWTPPEGNTTFIEGTT--------------WCVAKSGVSQIDLQ 78
           F G  T   +    T G +P   ++  I G +              WC+A S  S  +LQ
Sbjct: 345 FTGENTTPVSPTNSTTGTSPSPSSSPIINGNSTVTIGGGGGGGTKKWCIASSQASVTELQ 404

Query: 79  NALDWACGLGKADCKAIQKGGPCYQPDTLLSHASYAFNSYYQQNGNSDIACNFGGTATLT 138
            ALDWACG G  DC A+Q   PC++PDT+LSHASYAFN+YYQQ+G S I C+F G +   
Sbjct: 405 TALDWACGPGNVDCSAVQPDQPCFEPDTVLSHASYAFNTYYQQSGASSIDCSFNGASVEV 464

Query: 139 KKNPSYGKCDYSASSST 142
            K+PSYG C Y  + +T
Sbjct: 465 DKDPSYGNCLYMIAPAT 481

[Close]

BLAST of Cla97C03G057680 vs. ExPASy Swiss-Prot
Match: P52409 (Glucan endo-1,3-beta-glucosidase OS=Triticum aestivum OX=4565 GN=GLC1 PE=2 SV=1)

HSP 1 Score: 109.0 bits (271), Expect = 5.5e-23
Identity = 47/88 (53.41%), Postives = 62/88 (70.45%), Query Frame = 0


Query: 48  GTTWCVAKSGVSQIDLQNALDWACGLGKADCKAIQKGGPCYQPDTLLSHASYAFNSYYQQ 107
           G  WCVAK G +  DLQN +++ACG    DCK IQ GG C+ P++L +HASY  N+YYQ 
Sbjct: 374 GGKWCVAKDGANGTDLQNNINYACGF--VDCKPIQSGGACFSPNSLQAHASYVMNAYYQA 433

Query: 108 NGNSDIACNFGGTATLTKKNPSYGKCDY 136
           NG++D+AC+F GT  +T  +PSYG C Y
Sbjct: 434 NGHTDLACDFKGTGIVTSSDPSYGGCKY 459

[Close]

BLAST of Cla97C03G057680 vs. ExPASy Swiss-Prot
Match: Q9C7U5 (Glucan endo-1,3-beta-glucosidase 2 OS=Arabidopsis thaliana OX=3702 GN=At1g66250 PE=2 SV=2)

HSP 1 Score: 105.5 bits (262), Expect = 6.1e-22
Identity = 52/114 (45.61%), Postives = 73/114 (64.04%), Query Frame = 0


Query: 50  TWCVAKSGVSQIDLQNALDWACGLGKADCKAIQKGGPCYQPDTLLSHASYAFNSYYQQNG 109
           T+C A+ G     LQ ALDWACG GK DC  I++G  CY+PD +++HA+YAF++YY Q G
Sbjct: 367 TYCTAREGADTKMLQAALDWACGPGKIDCSPIKQGETCYEPDNVVAHANYAFDTYYHQTG 426

Query: 110 NSDIACNFGGTATLTKKNPSYGKCDYSAS--------SSTSSAPSKSKNSSSQI 156
           N+  ACNF G A++T  +PS+G C ++ S        S   +APS +  +SS I
Sbjct: 427 NNPDACNFNGVASITTTDPSHGTCVFAGSRGNGRNGTSVNITAPSANSTTSSGI 480

[Close]

BLAST of Cla97C03G057680 vs. ExPASy Swiss-Prot
Match: Q9M2K6 (PLASMODESMATA CALLOSE-BINDING PROTEIN 5 OS=Arabidopsis thaliana OX=3702 GN=PDCB5 PE=2 SV=2)

HSP 1 Score: 102.8 bits (255), Expect = 3.9e-21
Identity = 47/98 (47.96%), Postives = 59/98 (60.20%), Query Frame = 0


Query: 46  IEGTTWCVAKSGVSQIDLQNALDWACGLGKADCKAIQKGGPCYQPDTLLSHASYAFNSYY 105
           I+   WCVAK+      LQ A++WACG G ADC  IQ+GGPC  P  +   AS+ FN+YY
Sbjct: 36  IQVELWCVAKNNAEDSSLQTAIEWACGQGGADCGPIQQGGPCNDPTDVQKMASFVFNNYY 95

Query: 106 QQNGNSDIACNFGGTATLTKKNPSYGKCDYSASSSTSS 144
            +NG  D ACNF   A LT  NPS G C Y +S   ++
Sbjct: 96  LKNGEEDEACNFNNNAALTSLNPSQGTCKYPSSKGANN 133

[Close]

BLAST of Cla97C03G057680 vs. ExPASy TrEMBL
Match: A0A1S3BB84 (glucan endo-1,3-beta-glucosidase 13 OS=Cucumis melo OX=3656 GN=LOC103488047 PE=4 SV=1)

HSP 1 Score: 293.1 bits (749), Expect = 7.6e-76
Identity = 146/173 (84.39%), Postives = 159/173 (91.91%), Query Frame = 0


Query: 1   MRRKMWVLEIIILLLHYFFVGGATAAEAGMPVTAGWTPPEGNTTFIEGTTWCVAKSGVSQ 60
           M+ K+WVLE I+L LHYFF+GGA AAEAGMPV A   P EGNTTFI+GTTWCVAK GVSQ
Sbjct: 1   MKTKIWVLE-ILLFLHYFFLGGAMAAEAGMPVMA--APVEGNTTFIDGTTWCVAKPGVSQ 60

Query: 61  IDLQNALDWACGLGKADCKAIQKGGPCYQPDTLLSHASYAFNSYYQQNGNSDIACNFGGT 120
           IDLQNALDWACG+GKADC+AIQKGG CY+PDTLLSHASYAFNSYYQQNGNSDIACNFGG 
Sbjct: 61  IDLQNALDWACGMGKADCRAIQKGGRCYEPDTLLSHASYAFNSYYQQNGNSDIACNFGGC 120

Query: 121 ATLTKKNPSYGKCDYSASSSTSSAPSKSKNSSSQICMKLLSGVFMLLNLYFGS 174
           ATLTKK+PSYGKCDYSASSST+SAPSKSKNS S IC+KLLSGVF++LNLYFGS
Sbjct: 121 ATLTKKDPSYGKCDYSASSSTNSAPSKSKNSPSHICLKLLSGVFVVLNLYFGS 170

[Close]

BLAST of Cla97C03G057680 vs. ExPASy TrEMBL
Match: A0A0A0M121 (X8 domain-containing protein OS=Cucumis sativus OX=3659 GN=Csa_1G537410 PE=4 SV=1)

HSP 1 Score: 268.1 bits (684), Expect = 2.6e-68
Identity = 134/174 (77.01%), Postives = 151/174 (86.78%), Query Frame = 0


Query: 1   MRRKMWVLEIIILLLHYFFVGGATAAEAGMPVTAGWTPPEGNTTFIEGTTWCVAKSGVSQ 60
           M+ K+WVLEI++L LHYFF+GGA AAEAGMPV A   P EGNTTFI+GTTWCVAK+GVSQ
Sbjct: 1   MKTKIWVLEILLLFLHYFFLGGAMAAEAGMPVMA--APAEGNTTFIDGTTWCVAKAGVSQ 60

Query: 61  IDLQNALDWACGLGKADCKAIQKGGPCYQPDTLLSHASYAFNSYYQQNGNSDIACNFGGT 120
           IDLQNALDWACG+GKADC+AIQKGG CY+PDTLLSHAS+AFNSYYQQNGNSDIACNFGG 
Sbjct: 61  IDLQNALDWACGMGKADCRAIQKGGRCYEPDTLLSHASFAFNSYYQQNGNSDIACNFGG- 120

Query: 121 ATLTKKNPSYGKCDYSASSSTSSAPSKSKNSSSQICMKLLSGVFMLLNLYFGSS 175
                    YGKCDYSASSST+SAPSKSKNS + IC+KL+S V ++LNLYFGSS
Sbjct: 121 ---------YGKCDYSASSSTNSAPSKSKNSPTHICLKLVSVVSVVLNLYFGSS 162

[Close]

BLAST of Cla97C03G057680 vs. ExPASy TrEMBL
Match: A0A6J1CF38 (major pollen allergen Ole e 10 OS=Momordica charantia OX=3673 GN=LOC111010934 PE=4 SV=1)

HSP 1 Score: 252.7 bits (644), Expect = 1.1e-63
Identity = 126/180 (70.00%), Postives = 149/180 (82.78%), Query Frame = 0


Query: 1   MRRKMWVLEIII---LLLHYFFVGGATA----AEAGMPVTAGWTPPEGNTTFIEGTTWCV 60
           MR K+WVL +++    LL   F+G   A    AEA MP T   +PPEGN TF++GT+WCV
Sbjct: 1   MRTKIWVLLLVLEAMFLLQCCFLGEGMAVEEKAEAAMPATT-LSPPEGNATFLDGTSWCV 60

Query: 61  AKSGVSQIDLQNALDWACGLGKADCKAIQKGGPCYQPDTLLSHASYAFNSYYQQNGNSDI 120
           A++G SQIDLQNALDWACGLGKADC+AIQKGG CYQPDTLLSHASYAFNS+YQQNGNSDI
Sbjct: 61  ARAGASQIDLQNALDWACGLGKADCRAIQKGGACYQPDTLLSHASYAFNSFYQQNGNSDI 120

Query: 121 ACNFGGTATLTKKNPSYGKCDYSASSSTSSAPSKSKNSSSQICMKLLSGVFMLLNLYFGS 174
           ACNFGG+ATLTKKNPSYGKCDYSASSS +SA SK KN+ S +C++L++G+ M+LNLY GS
Sbjct: 121 ACNFGGSATLTKKNPSYGKCDYSASSSVNSATSKLKNNGSHVCLRLVAGILMVLNLYLGS 179

[Close]

BLAST of Cla97C03G057680 vs. ExPASy TrEMBL
Match: A0A6J1K6E1 (major pollen allergen Ole e 10-like OS=Cucurbita maxima OX=3661 GN=LOC111490492 PE=4 SV=1)

HSP 1 Score: 251.1 bits (640), Expect = 3.3e-63
Identity = 126/180 (70.00%), Postives = 149/180 (82.78%), Query Frame = 0


Query: 1   MRRKMWVLEIIILLLHYFFVGGATA-------AEAGMPVTAGWTPPEGNTTFIEGTTWCV 60
           M  ++W L + + LL  F +G A A       AEA MP+T  W+PPEGNTTF++GT+WCV
Sbjct: 1   MSIQIWGL-VALFLLQSFLMGEAMATAVAVEKAEASMPMTT-WSPPEGNTTFLDGTSWCV 60

Query: 61  AKSGVSQIDLQNALDWACGLGKADCKAIQKGGPCYQPDTLLSHASYAFNSYYQQNGNSDI 120
           AK GVSQIDLQNALDWACGLGKADC+AIQKGG CYQPDTL SHASYAFNSYYQQNGNSDI
Sbjct: 61  AKPGVSQIDLQNALDWACGLGKADCRAIQKGGRCYQPDTLFSHASYAFNSYYQQNGNSDI 120

Query: 121 ACNFGGTATLTKKNPSYGKCDYSASSSTSSAPSKSKNSSSQICMKLLSGVFMLLNLYFGS 174
           ACNFGG+ATLTK++PSYGKCDYSAS S SSAPSK K + S+ C++L+ G++M++NLYFGS
Sbjct: 121 ACNFGGSATLTKRDPSYGKCDYSASRSASSAPSKWKKNGSRGCLQLVGGIWMVMNLYFGS 178

[Close]

BLAST of Cla97C03G057680 vs. ExPASy TrEMBL
Match: A0A6J1EHM6 (glucan endo-1,3-beta-glucosidase 13-like isoform X1 OS=Cucurbita moschata OX=3662 GN=LOC111434256 PE=4 SV=1)

HSP 1 Score: 248.4 bits (633), Expect = 2.1e-62
Identity = 129/176 (73.30%), Postives = 146/176 (82.95%), Query Frame = 0


Query: 1   MRRKMWVLEIIILLLHYFFVGGATAAEAGMPVTAGWTPPEGNTTFIEGTTWCVAKSGVSQ 60
           M  K+WVLEI++LL H FFVG  TAA AG+PVT  WTP EGN T  EG +WCVAK GVSQ
Sbjct: 1   MGTKIWVLEILLLLQH-FFVGEVTAAAAGVPVTRTWTPSEGNATGAEGGSWCVAKPGVSQ 60

Query: 61  IDLQNALDWACGLGKADCKAIQKGGPCYQPDTLLSHASYAFNSYYQQNGNSDIACNFGGT 120
           IDLQNALDWACGLGKADC+AIQKGGPCYQPDTLLSHASYAFN +Y+QN NSD+ACNFGG+
Sbjct: 61  IDLQNALDWACGLGKADCRAIQKGGPCYQPDTLLSHASYAFNRFYKQNANSDMACNFGGS 120

Query: 121 ATLTKKNPSYGKCDYSASSSTSSAPSKSKNSSSQICMKL--LSGVF-MLLNLYFGS 174
           ATLT K+PSYGKCDYSASSS++SA  K KNS S  C+KL  L G+  ++LNLY GS
Sbjct: 121 ATLTNKDPSYGKCDYSASSSSASAEGK-KNSPSHGCLKLGMLCGILVVILNLYLGS 174

[Close]

BLAST of Cla97C03G057680 vs. TAIR 10
Match: AT4G05430.1 (Carbohydrate-binding X8 domain superfamily protein )

HSP 1 Score: 164.9 bits (416), Expect = 6.0e-41
Identity = 75/130 (57.69%), Postives = 96/130 (73.85%), Query Frame = 0


Query: 39  PEGNTTFIEGTTWCVAKSGVSQIDLQNALDWACGLGKADCKAIQKGGPCYQPDTLLSHAS 98
           P+GNTTF+EGTTWCVA+ G +Q +LQ ALDWACG+G+ DC  I++ G CY+PDT++SHAS
Sbjct: 11  PQGNTTFLEGTTWCVARPGATQAELQRALDWACGIGRVDCSVIERHGDCYEPDTIVSHAS 70

Query: 99  YAFNSYYQQNGNSDIACNFGGTATLTKKNPSYGKCDYSASSSTSSAPSKSKNSSSQICMK 158
           +AFN+YYQ NGN+ IAC FGGTAT TK NPSYGKC Y AS S  S         ++  + 
Sbjct: 71  FAFNAYYQTNGNNRIACYFGGTATFTKINPSYGKCSYDASKSEVSTARSLSEYKARWLLL 130

Query: 159 LLSGVFMLLN 169
           +  GVF L++
Sbjct: 131 MYIGVFSLIS 140

[Close]

BLAST of Cla97C03G057680 vs. TAIR 10
Match: AT5G56590.1 (O-Glycosyl hydrolases family 17 protein )

HSP 1 Score: 119.4 bits (298), Expect = 2.9e-27
Identity = 63/142 (44.37%), Postives = 86/142 (60.56%), Query Frame = 0


Query: 41  GNTTFIEGTTWCVAKSGVSQIDLQNALDWACGLGKADCKAIQKGGPCYQPDTLLSHASYA 100
           G  +     +WC+A S  S+ DL+ ALDWACG G  DC AIQ   PC+QPDTL+SHAS+ 
Sbjct: 359 GTNSSGSSNSWCIASSKASERDLKGALDWACGPGNVDCTAIQPSQPCFQPDTLVSHASFV 418

Query: 101 FNSYYQQNGNSDIACNFGGTATLTKKNPSYGKCDY-----------SASSSTSSAPSKSK 160
           FNSY+QQN  +D+AC+FGG      K+PSY KC Y           +A++ TSSA +   
Sbjct: 419 FNSYFQQNRATDVACSFGGAGVKVNKDPSYDKCIYITAGGNKTKATNATALTSSASTPRG 478

Query: 161 NSSSQICMKLLSGVFMLLNLYF 172
           N   Q  +KL     ++++L+F
Sbjct: 479 NELLQWILKLC----LMISLFF 496

[Close]

BLAST of Cla97C03G057680 vs. TAIR 10
Match: AT4G29360.1 (O-Glycosyl hydrolases family 17 protein )

HSP 1 Score: 117.9 bits (294), Expect = 8.4e-27
Identity = 59/137 (43.07%), Postives = 77/137 (56.20%), Query Frame = 0


Query: 19  FVGGATAAEAGMPVTAGWTPPEGNTTFIEGTT--------------WCVAKSGVSQIDLQ 78
           F G  T   +    T G +P   ++  I G +              WC+A S  S  +LQ
Sbjct: 345 FTGENTTPVSPTNSTTGTSPSPSSSPIINGNSTVTIGGGGGGGTKKWCIASSQASVTELQ 404

Query: 79  NALDWACGLGKADCKAIQKGGPCYQPDTLLSHASYAFNSYYQQNGNSDIACNFGGTATLT 138
            ALDWACG G  DC A+Q   PC++PDT+LSHASYAFN+YYQQ+G S I C+F G +   
Sbjct: 405 TALDWACGPGNVDCSAVQPDQPCFEPDTVLSHASYAFNTYYQQSGASSIDCSFNGASVEV 464

Query: 139 KKNPSYGKCDYSASSST 142
            K+PSYG C Y  + +T
Sbjct: 465 DKDPSYGNCLYMIAPAT 481

[Close]

BLAST of Cla97C03G057680 vs. TAIR 10
Match: AT1G09460.1 (Carbohydrate-binding X8 domain superfamily protein )

HSP 1 Score: 117.5 bits (293), Expect = 1.1e-26
Identity = 60/114 (52.63%), Postives = 76/114 (66.67%), Query Frame = 0


Query: 30  MPVTAGWTPPEGNTTFIEGTTWCVAKSGVSQIDLQNALDWACGLGKADCKAIQKGGPCYQ 89
           +PV     P   N+  + G +WCVAK G SQ+ LQ ALD+ACG+  ADC  +Q+GG CY 
Sbjct: 116 VPVPVVAPPVVSNSPSVSGQSWCVAKPGASQVSLQQALDYACGI--ADCSQLQQGGNCYS 175

Query: 90  PDTLLSHASYAFNSYYQQNGNSDIACNFGGTATLTKKNPSYGKCDYSASSSTSS 144
           P +L SHAS+AFNSYYQ+N  S  +C+FGG A+L   NPS G C Y   SSTS+
Sbjct: 176 PISLQSHASFAFNSYYQKN-PSPQSCDFGGAASLVNTNPSTGSCIYQTGSSTST 226

[Close]

BLAST of Cla97C03G057680 vs. TAIR 10
Match: AT1G29380.1 (Carbohydrate-binding X8 domain superfamily protein )

HSP 1 Score: 111.7 bits (278), Expect = 6.0e-25
Identity = 62/134 (46.27%), Postives = 79/134 (58.96%), Query Frame = 0


Query: 21  GGATAAEAGMPVTAGWTPPEGNTTFIEGT---TWCVAKSGVSQIDLQNALDWACGLGKAD 80
           GG    + G     G     G+T    G     WC+AK+  S   LQ ALD+ACG G AD
Sbjct: 115 GGGGGGDTGAGAGGGGYGGGGDTGAGGGVGSGQWCIAKANASPTSLQVALDYACGYGGAD 174

Query: 81  CKAIQKGGPCYQPDTLLSHASYAFNSYYQQNGNSDIACNFGGTATLTKKNPSYGKCDYSA 140
           C  IQ+G  CY+P+T+  HAS+AFNSYYQ++  SD +CNFGG A LT  +PS G C +S+
Sbjct: 175 CGQIQQGAACYEPNTIRDHASFAFNSYYQKHPGSD-SCNFGGAAQLTSTDPSKGSCHFSS 234

Query: 141 SSSTSSAPSKSKNS 152
           SS T S    S+ S
Sbjct: 235 SSGTVSTSPPSQMS 247

The following BLAST results are available for this feature:

BLAST of Cla97C03G057680 vs. NCBI nr


Match Name	E-value	Identity	Description
XP_038895270.1	1.5e-78	86.29	major pollen allergen Ole e 10 [Benincasa hispida]	[more]
XP_008444815.1	1.6e-75	84.39	PREDICTED: glucan endo-1,3-beta-glucosidase 13 [Cucumis melo]	[more]
XP_004135538.1	1.0e-74	81.61	major pollen allergen Ole e 10 [Cucumis sativus] >KAE8653300.1 hypothetical prot...	[more]
XP_022140209.1	2.3e-63	70.00	major pollen allergen Ole e 10 [Momordica charantia]	[more]
XP_022994908.1	6.8e-63	70.00	major pollen allergen Ole e 10-like [Cucurbita maxima]	[more]

BLAST of Cla97C03G057680 vs. ExPASy Swiss-Prot


Match Name	E-value	Identity	Description
Q9FJU9	4.1e-26	44.37	Glucan endo-1,3-beta-glucosidase 13 OS=Arabidopsis thaliana OX=3702 GN=At5g56590...	[more]
Q8VYE5	1.2e-25	43.07	Glucan endo-1,3-beta-glucosidase 12 OS=Arabidopsis thaliana OX=3702 GN=At4g29360...	[more]
P52409	5.5e-23	53.41	Glucan endo-1,3-beta-glucosidase OS=Triticum aestivum OX=4565 GN=GLC1 PE=2 SV=1	[more]
Q9C7U5	6.1e-22	45.61	Glucan endo-1,3-beta-glucosidase 2 OS=Arabidopsis thaliana OX=3702 GN=At1g66250 ...	[more]
Q9M2K6	3.9e-21	47.96	PLASMODESMATA CALLOSE-BINDING PROTEIN 5 OS=Arabidopsis thaliana OX=3702 GN=PDCB5...	[more]

BLAST of Cla97C03G057680 vs. ExPASy TrEMBL


Match Name	E-value	Identity	Description
A0A1S3BB84	7.6e-76	84.39	glucan endo-1,3-beta-glucosidase 13 OS=Cucumis melo OX=3656 GN=LOC103488047 PE=4...	[more]
A0A0A0M121	2.6e-68	77.01	X8 domain-containing protein OS=Cucumis sativus OX=3659 GN=Csa_1G537410 PE=4 SV=...	[more]
A0A6J1CF38	1.1e-63	70.00	major pollen allergen Ole e 10 OS=Momordica charantia OX=3673 GN=LOC111010934 PE...	[more]
A0A6J1K6E1	3.3e-63	70.00	major pollen allergen Ole e 10-like OS=Cucurbita maxima OX=3661 GN=LOC111490492 ...	[more]
A0A6J1EHM6	2.1e-62	73.30	glucan endo-1,3-beta-glucosidase 13-like isoform X1 OS=Cucurbita moschata OX=366...	[more]

BLAST of Cla97C03G057680 vs. TAIR 10


Match Name	E-value	Identity	Description
AT4G05430.1	6.0e-41	57.69	Carbohydrate-binding X8 domain superfamily protein	[more]
AT5G56590.1	2.9e-27	44.37	O-Glycosyl hydrolases family 17 protein	[more]
AT4G29360.1	8.4e-27	43.07	O-Glycosyl hydrolases family 17 protein	[more]
AT1G09460.1	1.1e-26	52.63	Carbohydrate-binding X8 domain superfamily protein	[more]
AT1G29380.1	6.0e-25	46.27	Carbohydrate-binding X8 domain superfamily protein	[more]

InterPro

Analysis Name: InterPro Annotations of Watermelon (97103) v2.5
Date Performed: 2022-01-31


IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
IPR012946	X8 domain	SMART	SM00768	X8_cls	coord: 50..135 e-value: 1.7E-50 score: 183.7
IPR012946	X8 domain	PFAM	PF07983	X8	coord: 50..122 e-value: 9.9E-24 score: 83.8
None	No IPR available	GENE3D	1.20.58.1040		coord: 44..130 e-value: 1.2E-13 score: 53.3
None	No IPR available	PANTHER	PTHR31044:SF36	CARBOHYDRATE-BINDING X8 DOMAIN SUPERFAMILY PROTEIN	coord: 32..136
IPR044788	Carbohydrate-binding X8 domain-containing protein, plant	PANTHER	PTHR31044	BETA-1,3 GLUCANASE	coord: 32..136

Relationships

The following mRNA feature(s) are a part of this gene:


Feature Name	Unique Name	Type
Cla97C03G057680.1	Cla97C03G057680.1	mRNA

GO Annotation

GO Assignments

This gene is annotated with the following GO terms.

Category	Term Accession	Term Name
cellular_component	GO:0009506	plasmodesma