Cla97C03G054315 (gene) Watermelon (97103) v2.5

Overview


Name	Cla97C03G054315
Type	gene
Organism	Citrullus lanatus subsp. vulgaris cv. 97103 (Watermelon (97103) v2.5)
Description	Pectinesterase
Location	Cla97Chr03: 3545627 .. 3546499 (-)
RNA-Seq Expression	Cla97C03G054315
Synteny	Cla97C03G054315

Sequences

The following sequences are available for this feature:

Gene sequence (with intron)

Legend: polypeptideexonCDS

Hold the cursor over a type above to highlight its positions in the sequence below.

ATGAAGGTCTTTTTACAACAACTATCATGTGCCTTTCTTGCATGATTTTCCTTTGCATGGTGATAAATTCAATTATAATTCATCACTAATTTTTTTTCATATCACATTAACTCATCCCTTTTTCGTTAGAAGATAAATCTCTTCTTATTTCTTAATTAATTGAACTCGTTACATTATTTGAATGAGATAGATGACTATTTTTCTATAGTTAAGAATTATAATAGAAATAGGACGACTTGGTGATTTTATTTGTAAAGATCAATCCTTGAAAAAAACTCATTAATTCCCTTCACAGTTAAGTTTAATGCTACTAATTAATTTGTATTTAGTGAATAAATAATGCATTGGTTTGTGATAAAAACGGTGTGATGATTGCACATATTGCTTATCAGAATAATGGAACTAAATTGAAGTTGCAACTTCTAAAAAATATCTTCATTAAGCATTAAATCCCTAACTAAGTGAGATTAGCTCCACAAAATTACCTTTTGTTTTTAGTATTAGTGTTAACATTGAAAATGAGAGCTACAAGTACCTATCTACCTCTATAAAAAGTGAGAGCTTCAAGCCCTTTATACCATTTCATTCTTTTCCTTCTCTCCAACTTTAAACAATATGGAATTTTTCAATGTTGTTGCAAATTTTCTTTTTTGATTCTTCAACCTGGTGATATCCGTTCATTGCATCAATTTTAATGTATCTGTATCACTTGACGGTTCCAAAGATTTCGGAAGCATCAATGACGATGCAATTGCAGCGGCTCCTGATAACTCTAATACACGATTTTACATTCGTGTAACCCCTGGCACTTATCATGAACGTCTTCAAATCCCAACTTCCAAGACTTTCATTGCTTTGATTGGTGATAATTAA

mRNA sequence

ATGAAGAATAATGGAACTAAATTGAATATTAGTGTTAACATTGAAAATGAGAGCTACAAGTACCTATCTACCTCTATAAAAAATTTCGGAAGCATCAATGACGATGCAATTGCAGCGGCTCCTGATAACTCTAATACACGATTTTACATTCGTGTAACCCCTGGCACTTATCATGAACGTCTTCAAATCCCAACTTCCAAGACTTTCATTGCTTTGATTGGTGATAATTAA

Coding sequence (CDS)

ATGAAGAATAATGGAACTAAATTGAATATTAGTGTTAACATTGAAAATGAGAGCTACAAGTACCTATCTACCTCTATAAAAAATTTCGGAAGCATCAATGACGATGCAATTGCAGCGGCTCCTGATAACTCTAATACACGATTTTACATTCGTGTAACCCCTGGCACTTATCATGAACGTCTTCAAATCCCAACTTCCAAGACTTTCATTGCTTTGATTGGTGATAATTAA

Protein sequence

MKNNGTKLNISVNIENESYKYLSTSIKNFGSINDDAIAAAPDNSNTRFYIRVTPGTYHERLQIPTSKTFIALIGDN

Homology

[Close]

BLAST of Cla97C03G054315 vs. NCBI nr
Match: KAA0055287.1 (putative pectinesterase/pectinesterase inhibitor 41 [Cucumis melo var. makuwa] >TYJ99210.1 putative pectinesterase/pectinesterase inhibitor 41 [Cucumis melo var. makuwa])

HSP 1 Score: 85.5 bits (210), Expect = 2.2e-13
Identity = 43/52 (82.69%), Postives = 45/52 (86.54%), Query Frame = 0


Query: 30 GSIND-----DAIAAAPDNSNTRFYIRVTPGTYHERLQIPTSKTFIALIGDN 77
          GS+ND      AIAAAPDNSNTRFYIRVTPGTYHERLQIP +KTFIALIGDN
Sbjct: 34 GSMNDFKTIAAAIAAAPDNSNTRFYIRVTPGTYHERLQIPPTKTFIALIGDN 85

[Close]

BLAST of Cla97C03G054315 vs. NCBI nr
Match: KAA0055288.1 (putative pectinesterase/pectinesterase inhibitor 41 [Cucumis melo var. makuwa] >TYJ99211.1 putative pectinesterase/pectinesterase inhibitor 41 [Cucumis melo var. makuwa])

HSP 1 Score: 83.6 bits (205), Expect = 8.2e-13
Identity = 43/55 (78.18%), Postives = 47/55 (85.45%), Query Frame = 0


Query: 22 LSTSIKNFGSINDDAIAAAPDNSNTRFYIRVTPGTYHERLQIPTSKTFIALIGDN 77
          L  S+ +F +I   AIAAAPDNSNTRFYIRVTPGTYHERLQIP +KTFIALIGDN
Sbjct: 32 LDGSMNHFKTI-AAAIAAAPDNSNTRFYIRVTPGTYHERLQIPPTKTFIALIGDN 85

[Close]

BLAST of Cla97C03G054315 vs. NCBI nr
Match: XP_016903572.1 (PREDICTED: probable pectinesterase/pectinesterase inhibitor 40 [Cucumis melo])

HSP 1 Score: 80.9 bits (198), Expect = 5.3e-12
Identity = 36/42 (85.71%), Postives = 39/42 (92.86%), Query Frame = 0


Query: 35 DAIAAAPDNSNTRFYIRVTPGTYHERLQIPTSKTFIALIGDN 77
          DAI AAPDNSNT+FYI VTPGTYHERLQIP +KTF+ALIGDN
Sbjct: 9  DAIVAAPDNSNTQFYIHVTPGTYHERLQIPPTKTFVALIGDN 50

[Close]

BLAST of Cla97C03G054315 vs. NCBI nr
Match: TYJ99206.1 (putative pectinesterase/pectinesterase inhibitor 41 [Cucumis melo var. makuwa])

HSP 1 Score: 80.9 bits (198), Expect = 5.3e-12
Identity = 36/42 (85.71%), Postives = 39/42 (92.86%), Query Frame = 0


Query: 35 DAIAAAPDNSNTRFYIRVTPGTYHERLQIPTSKTFIALIGDN 77
          DAI AAPDNSNT+FYI VTPGTYHERLQIP +KTF+ALIGDN
Sbjct: 9  DAIVAAPDNSNTQFYIHVTPGTYHERLQIPPTKTFVALIGDN 50

[Close]

BLAST of Cla97C03G054315 vs. NCBI nr
Match: KAA0055283.1 (putative pectinesterase/pectinesterase inhibitor 41 [Cucumis melo var. makuwa])

HSP 1 Score: 79.0 bits (193), Expect = 2.0e-11
Identity = 37/42 (88.10%), Postives = 38/42 (90.48%), Query Frame = 0


Query: 35 DAIAAAPDNSNTRFYIRVTPGTYHERLQIPTSKTFIALIGDN 77
          DAI AA DNSNTRFYI VTPGTYHERLQIP +KTFIALIGDN
Sbjct: 9  DAIVAALDNSNTRFYIHVTPGTYHERLQIPPTKTFIALIGDN 50

[Close]

BLAST of Cla97C03G054315 vs. ExPASy Swiss-Prot
Match: O81415 (Probable pectinesterase/pectinesterase inhibitor 39 OS=Arabidopsis thaliana OX=3702 GN=PME39 PE=2 SV=1)

HSP 1 Score: 47.8 bits (112), Expect = 6.5e-05
Identity = 29/70 (41.43%), Postives = 38/70 (54.29%), Query Frame = 0


Query: 6   TKLNISVNIENESYKYLSTSIKNFGSINDDAIAAAPDNSNTRFYIRVTPGTYHERLQIPT 65
           T  N+SV I+            NF +IN DA+ AAP+ S TRF I +  G Y E +++P 
Sbjct: 218 TNYNLSVAIDGTG---------NFTTIN-DAVFAAPNMSETRFIIYIKGGEYFENVELPK 277

Query: 66  SKTFIALIGD 76
            KT I  IGD
Sbjct: 278 KKTMIMFIGD 277

[Close]

BLAST of Cla97C03G054315 vs. ExPASy Swiss-Prot
Match: O81301 (Probable pectinesterase/pectinesterase inhibitor 40 OS=Arabidopsis thaliana OX=3702 GN=PME40 PE=2 SV=1)

HSP 1 Score: 47.0 bits (110), Expect = 1.1e-04
Identity = 21/41 (51.22%), Postives = 28/41 (68.29%), Query Frame = 0


Query: 35  DAIAAAPDNSNTRFYIRVTPGTYHERLQIPTSKTFIALIGD 76
           +AI+AAP++S TRF I +  G Y E ++IP  KT I  IGD
Sbjct: 223 EAISAAPNSSETRFVIYIKCGEYFENIEIPREKTMIMFIGD 263

[Close]

BLAST of Cla97C03G054315 vs. ExPASy Swiss-Prot
Match: Q1JPL7 (Pectinesterase/pectinesterase inhibitor 18 OS=Arabidopsis thaliana OX=3702 GN=PME18 PE=1 SV=3)

HSP 1 Score: 45.4 bits (106), Expect = 3.2e-04
Identity = 22/47 (46.81%), Postives = 31/47 (65.96%), Query Frame = 0


Query: 29  FGSINDDAIAAAPDNSNTRFYIRVTPGTYHERLQIPTSKTFIALIGD 76
           F ++N +A+AAAP+NSNTR+ I V  G Y E + I   K  + L+GD
Sbjct: 257 FKTVN-EAVAAAPENSNTRYVIYVKKGVYKETIDIGKKKKNLMLVGD 302

[Close]

BLAST of Cla97C03G054315 vs. ExPASy Swiss-Prot
Match: P83218 (Pectinesterase OS=Daucus carota OX=4039 PE=1 SV=1)

HSP 1 Score: 45.4 bits (106), Expect = 3.2e-04
Identity = 18/41 (43.90%), Postives = 27/41 (65.85%), Query Frame = 0


Query: 35 DAIAAAPDNSNTRFYIRVTPGTYHERLQIPTSKTFIALIGD 76
          +A+AAAP++S TR+ IR+  G Y E + +P  K  I  +GD
Sbjct: 24 EAVAAAPEDSKTRYVIRIKAGVYRENVDVPKKKKNIMFLGD 64

[Close]

BLAST of Cla97C03G054315 vs. ExPASy Swiss-Prot
Match: P14280 (Pectinesterase 1 OS=Solanum lycopersicum OX=4081 GN=PME1.9 PE=1 SV=5)

HSP 1 Score: 44.7 bits (104), Expect = 5.5e-04
Identity = 18/41 (43.90%), Postives = 29/41 (70.73%), Query Frame = 0


Query: 35  DAIAAAPDNSNTRFYIRVTPGTYHERLQIPTSKTFIALIGD 76
           +A+AAAPD S TR+ I V  GTY E +++ ++K  + ++GD
Sbjct: 249 EAVAAAPDKSKTRYVIYVKRGTYKENVEVASNKMNLMIVGD 289

[Close]

BLAST of Cla97C03G054315 vs. ExPASy TrEMBL
Match: A0A5D3BHF7 (Pectinesterase OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold248G004200 PE=4 SV=1)

HSP 1 Score: 85.5 bits (210), Expect = 1.0e-13
Identity = 43/52 (82.69%), Postives = 45/52 (86.54%), Query Frame = 0


Query: 30 GSIND-----DAIAAAPDNSNTRFYIRVTPGTYHERLQIPTSKTFIALIGDN 77
          GS+ND      AIAAAPDNSNTRFYIRVTPGTYHERLQIP +KTFIALIGDN
Sbjct: 34 GSMNDFKTIAAAIAAAPDNSNTRFYIRVTPGTYHERLQIPPTKTFIALIGDN 85

[Close]

BLAST of Cla97C03G054315 vs. ExPASy TrEMBL
Match: A0A5D3BKF8 (Pectinesterase OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold248G004210 PE=4 SV=1)

HSP 1 Score: 83.6 bits (205), Expect = 4.0e-13
Identity = 43/55 (78.18%), Postives = 47/55 (85.45%), Query Frame = 0


Query: 22 LSTSIKNFGSINDDAIAAAPDNSNTRFYIRVTPGTYHERLQIPTSKTFIALIGDN 77
          L  S+ +F +I   AIAAAPDNSNTRFYIRVTPGTYHERLQIP +KTFIALIGDN
Sbjct: 32 LDGSMNHFKTI-AAAIAAAPDNSNTRFYIRVTPGTYHERLQIPPTKTFIALIGDN 85

[Close]

BLAST of Cla97C03G054315 vs. ExPASy TrEMBL
Match: A0A5D3BJD0 (Pectinesterase OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold248G004160 PE=4 SV=1)

HSP 1 Score: 80.9 bits (198), Expect = 2.6e-12
Identity = 36/42 (85.71%), Postives = 39/42 (92.86%), Query Frame = 0


Query: 35 DAIAAAPDNSNTRFYIRVTPGTYHERLQIPTSKTFIALIGDN 77
          DAI AAPDNSNT+FYI VTPGTYHERLQIP +KTF+ALIGDN
Sbjct: 9  DAIVAAPDNSNTQFYIHVTPGTYHERLQIPPTKTFVALIGDN 50

[Close]

BLAST of Cla97C03G054315 vs. ExPASy TrEMBL
Match: A0A1S4E5T0 (Pectinesterase OS=Cucumis melo OX=3656 GN=LOC107992235 PE=4 SV=1)

HSP 1 Score: 80.9 bits (198), Expect = 2.6e-12
Identity = 36/42 (85.71%), Postives = 39/42 (92.86%), Query Frame = 0


Query: 35 DAIAAAPDNSNTRFYIRVTPGTYHERLQIPTSKTFIALIGDN 77
          DAI AAPDNSNT+FYI VTPGTYHERLQIP +KTF+ALIGDN
Sbjct: 9  DAIVAAPDNSNTQFYIHVTPGTYHERLQIPPTKTFVALIGDN 50

[Close]

BLAST of Cla97C03G054315 vs. ExPASy TrEMBL
Match: A0A5A7UH51 (Pectinesterase OS=Cucumis melo var. makuwa OX=1194695 GN=E6C27_scaffold80G001260 PE=4 SV=1)

HSP 1 Score: 79.0 bits (193), Expect = 9.8e-12
Identity = 37/42 (88.10%), Postives = 38/42 (90.48%), Query Frame = 0


Query: 35 DAIAAAPDNSNTRFYIRVTPGTYHERLQIPTSKTFIALIGDN 77
          DAI AA DNSNTRFYI VTPGTYHERLQIP +KTFIALIGDN
Sbjct: 9  DAIVAALDNSNTRFYIHVTPGTYHERLQIPPTKTFIALIGDN 50

[Close]

BLAST of Cla97C03G054315 vs. TAIR 10
Match: AT4G02300.1 (Plant invertase/pectin methylesterase inhibitor superfamily )

HSP 1 Score: 47.8 bits (112), Expect = 4.6e-06
Identity = 29/70 (41.43%), Postives = 38/70 (54.29%), Query Frame = 0


Query: 6   TKLNISVNIENESYKYLSTSIKNFGSINDDAIAAAPDNSNTRFYIRVTPGTYHERLQIPT 65
           T  N+SV I+            NF +IN DA+ AAP+ S TRF I +  G Y E +++P 
Sbjct: 218 TNYNLSVAIDGTG---------NFTTIN-DAVFAAPNMSETRFIIYIKGGEYFENVELPK 277

Query: 66  SKTFIALIGD 76
            KT I  IGD
Sbjct: 278 KKTMIMFIGD 277

[Close]

BLAST of Cla97C03G054315 vs. TAIR 10
Match: AT4G02320.1 (Plant invertase/pectin methylesterase inhibitor superfamily )

HSP 1 Score: 47.0 bits (110), Expect = 7.9e-06
Identity = 21/41 (51.22%), Postives = 28/41 (68.29%), Query Frame = 0


Query: 35  DAIAAAPDNSNTRFYIRVTPGTYHERLQIPTSKTFIALIGD 76
           +AI+AAP++S TRF I +  G Y E ++IP  KT I  IGD
Sbjct: 223 EAISAAPNSSETRFVIYIKCGEYFENIEIPREKTMIMFIGD 263

[Close]

BLAST of Cla97C03G054315 vs. TAIR 10
Match: AT1G11580.1 (methylesterase PCR A )

HSP 1 Score: 45.4 bits (106), Expect = 2.3e-05
Identity = 22/47 (46.81%), Postives = 31/47 (65.96%), Query Frame = 0


Query: 29  FGSINDDAIAAAPDNSNTRFYIRVTPGTYHERLQIPTSKTFIALIGD 76
           F ++N +A+AAAP+NSNTR+ I V  G Y E + I   K  + L+GD
Sbjct: 257 FKTVN-EAVAAAPENSNTRYVIYVKKGVYKETIDIGKKKKNLMLVGD 302

[Close]

BLAST of Cla97C03G054315 vs. TAIR 10
Match: AT1G53830.1 (pectin methylesterase 2 )

HSP 1 Score: 43.5 bits (101), Expect = 8.8e-05
Identity = 18/40 (45.00%), Postives = 25/40 (62.50%), Query Frame = 0


Query: 36  AIAAAPDNSNTRFYIRVTPGTYHERLQIPTSKTFIALIGD 76
           A+AAAP+ SN RF I +  G Y E +++   KT I  +GD
Sbjct: 293 AVAAAPEKSNKRFVIHIKAGVYRENVEVTKKKTNIMFLGD 332

[Close]

BLAST of Cla97C03G054315 vs. TAIR 10
Match: AT3G14310.1 (pectin methylesterase 3 )

HSP 1 Score: 42.0 bits (97), Expect = 2.6e-04
Identity = 17/40 (42.50%), Postives = 25/40 (62.50%), Query Frame = 0


Query: 36  AIAAAPDNSNTRFYIRVTPGTYHERLQIPTSKTFIALIGD 76
           A+AAAP+NSN R+ I +  G Y E +++   K  I  +GD
Sbjct: 298 AVAAAPENSNKRYVIHIKAGVYRENVEVAKKKKNIMFMGD 337

The following BLAST results are available for this feature:

BLAST of Cla97C03G054315 vs. NCBI nr


Match Name	E-value	Identity	Description
KAA0055287.1	2.2e-13	82.69	putative pectinesterase/pectinesterase inhibitor 41 [Cucumis melo var. makuwa] >...	[more]
KAA0055288.1	8.2e-13	78.18	putative pectinesterase/pectinesterase inhibitor 41 [Cucumis melo var. makuwa] >...	[more]
XP_016903572.1	5.3e-12	85.71	PREDICTED: probable pectinesterase/pectinesterase inhibitor 40 [Cucumis melo]	[more]
TYJ99206.1	5.3e-12	85.71	putative pectinesterase/pectinesterase inhibitor 41 [Cucumis melo var. makuwa]	[more]
KAA0055283.1	2.0e-11	88.10	putative pectinesterase/pectinesterase inhibitor 41 [Cucumis melo var. makuwa]	[more]

BLAST of Cla97C03G054315 vs. ExPASy Swiss-Prot


Match Name	E-value	Identity	Description
O81415	6.5e-05	41.43	Probable pectinesterase/pectinesterase inhibitor 39 OS=Arabidopsis thaliana OX=3...	[more]
O81301	1.1e-04	51.22	Probable pectinesterase/pectinesterase inhibitor 40 OS=Arabidopsis thaliana OX=3...	[more]
Q1JPL7	3.2e-04	46.81	Pectinesterase/pectinesterase inhibitor 18 OS=Arabidopsis thaliana OX=3702 GN=PM...	[more]
P83218	3.2e-04	43.90	Pectinesterase OS=Daucus carota OX=4039 PE=1 SV=1	[more]
P14280	5.5e-04	43.90	Pectinesterase 1 OS=Solanum lycopersicum OX=4081 GN=PME1.9 PE=1 SV=5	[more]

BLAST of Cla97C03G054315 vs. ExPASy TrEMBL


Match Name	E-value	Identity	Description
A0A5D3BHF7	1.0e-13	82.69	Pectinesterase OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold248G00420...	[more]
A0A5D3BKF8	4.0e-13	78.18	Pectinesterase OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold248G00421...	[more]
A0A5D3BJD0	2.6e-12	85.71	Pectinesterase OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold248G00416...	[more]
A0A1S4E5T0	2.6e-12	85.71	Pectinesterase OS=Cucumis melo OX=3656 GN=LOC107992235 PE=4 SV=1	[more]
A0A5A7UH51	9.8e-12	88.10	Pectinesterase OS=Cucumis melo var. makuwa OX=1194695 GN=E6C27_scaffold80G001260...	[more]

BLAST of Cla97C03G054315 vs. TAIR 10


Match Name	E-value	Identity	Description
AT4G02300.1	4.6e-06	41.43	Plant invertase/pectin methylesterase inhibitor superfamily	[more]
AT4G02320.1	7.9e-06	51.22	Plant invertase/pectin methylesterase inhibitor superfamily	[more]
AT1G11580.1	2.3e-05	46.81	methylesterase PCR A	[more]
AT1G53830.1	8.8e-05	45.00	pectin methylesterase 2	[more]
AT3G14310.1	2.6e-04	42.50	pectin methylesterase 3	[more]

InterPro

Analysis Name: InterPro Annotations of Watermelon (97103) v2.5
Date Performed: 2022-01-31


IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
IPR000070	Pectinesterase, catalytic	PFAM	PF01095	Pectinesterase	coord: 28..75 e-value: 1.4E-7 score: 30.5
IPR012334	Pectin lyase fold	GENE3D	2.160.20.10		coord: 4..76 e-value: 7.8E-12 score: 46.9
IPR011050	Pectin lyase fold/virulence factor	SUPERFAMILY	51126	Pectin lyase-like	coord: 27..76

Relationships

The following mRNA feature(s) are a part of this gene:


Feature Name	Unique Name	Type
Cla97C03G054315.1	Cla97C03G054315.1	mRNA

GO Annotation

GO Assignments

This gene is annotated with the following GO terms.

Category	Term Accession	Term Name
biological_process	GO:0042545	cell wall modification
biological_process	GO:0045490	pectin catabolic process
cellular_component	GO:0016021	integral component of membrane
molecular_function	GO:0045330	aspartyl esterase activity
molecular_function	GO:0030599	pectinesterase activity
molecular_function	GO:0046910	pectinesterase inhibitor activity