Cla97C02G041770 (gene) Watermelon (97103) v2.5

Overview


Name	Cla97C02G041770
Type	gene
Organism	Citrullus lanatus subsp. vulgaris cv. 97103 (Watermelon (97103) v2.5)
Description	Miraculin-like
Location	Cla97Chr02: 29652176 .. 29652806 (+)
RNA-Seq Expression	Cla97C02G041770
Synteny	Cla97C02G041770

Sequences

The following sequences are available for this feature:

Gene sequence (with intron)

Legend: exonfive_prime_UTRCDSpolypeptide

Hold the cursor over a type above to highlight its positions in the sequence below.

GTTTACACAAATTAAATTCTCAAGTACTTTGAAGAATAAGAAGATGATGATGCTGCATAAATCACTTGCCATCTTTGGCTATGCATGCCTCTTCATGGCCATAACTTCGATAGCTCAATTTCCTCCAGTTTTCGACACCGATGGTCAACCTCTGCGGCGGGGTGTCGAGTACTACATCTCGCCTGTATTTACCGATGTTGGGGGCAACCTCACCCTAAAAAGTCGTAGTAACGCTCCATGCCCGCTCTTCGTCGGCCAGGAACCGGTTACTTCAACGAATATCGGTCTCCCGGTCACCTTCACTCCCATCATGGCAGGCAAAGATATAATTGACGAAGGTACCAGTTTGAACATTGTGTTTCAAGCGTTGTCAACGTGCATAACATCGACGCAATGGAGAGTGGATGCGGCGGAGTCTGACACCGGGAGGAGGTTCGTGGGGATCGGGAGGGAGGACGGTCCTGCCGGGATCTTTGGGATCAGTAGGGACAATGGAGTTTACAATATAGTATGGTGTCCGGCGATGATGGGGAGGCCAAGGTGTGGAAGGGCTGGGATTTTGGTTGAGAATGGAGTGAGGCTGTTGGCTTTGGATGGTGAAGCGTTTCCTTTTGAGTTTGTGAAAGCTTGA

mRNA sequence

GTTTACACAAATTAAATTCTCAAGTACTTTGAAGAATAAGAAGATGATGATGCTGCATAAATCACTTGCCATCTTTGGCTATGCATGCCTCTTCATGGCCATAACTTCGATAGCTCAATTTCCTCCAGTTTTCGACACCGATGGTCAACCTCTGCGGCGGGGTGTCGAGTACTACATCTCGCCTGTATTTACCGATGTTGGGGGCAACCTCACCCTAAAAAGTCGTAGTAACGCTCCATGCCCGCTCTTCGTCGGCCAGGAACCGGTTACTTCAACGAATATCGGTCTCCCGGTCACCTTCACTCCCATCATGGCAGGCAAAGATATAATTGACGAAGGTACCAGTTTGAACATTGTGTTTCAAGCGTTGTCAACGTGCATAACATCGACGCAATGGAGAGTGGATGCGGCGGAGTCTGACACCGGGAGGAGGTTCGTGGGGATCGGGAGGGAGGACGGTCCTGCCGGGATCTTTGGGATCAGTAGGGACAATGGAGTTTACAATATAGTATGGTGTCCGGCGATGATGGGGAGGCCAAGGTGTGGAAGGGCTGGGATTTTGGTTGAGAATGGAGTGAGGCTGTTGGCTTTGGATGGTGAAGCGTTTCCTTTTGAGTTTGTGAAAGCTTGA

Coding sequence (CDS)

ATGATGATGCTGCATAAATCACTTGCCATCTTTGGCTATGCATGCCTCTTCATGGCCATAACTTCGATAGCTCAATTTCCTCCAGTTTTCGACACCGATGGTCAACCTCTGCGGCGGGGTGTCGAGTACTACATCTCGCCTGTATTTACCGATGTTGGGGGCAACCTCACCCTAAAAAGTCGTAGTAACGCTCCATGCCCGCTCTTCGTCGGCCAGGAACCGGTTACTTCAACGAATATCGGTCTCCCGGTCACCTTCACTCCCATCATGGCAGGCAAAGATATAATTGACGAAGGTACCAGTTTGAACATTGTGTTTCAAGCGTTGTCAACGTGCATAACATCGACGCAATGGAGAGTGGATGCGGCGGAGTCTGACACCGGGAGGAGGTTCGTGGGGATCGGGAGGGAGGACGGTCCTGCCGGGATCTTTGGGATCAGTAGGGACAATGGAGTTTACAATATAGTATGGTGTCCGGCGATGATGGGGAGGCCAAGGTGTGGAAGGGCTGGGATTTTGGTTGAGAATGGAGTGAGGCTGTTGGCTTTGGATGGTGAAGCGTTTCCTTTTGAGTTTGTGAAAGCTTGA

Protein sequence

MMMLHKSLAIFGYACLFMAITSIAQFPPVFDTDGQPLRRGVEYYISPVFTDVGGNLTLKSRSNAPCPLFVGQEPVTSTNIGLPVTFTPIMAGKDIIDEGTSLNIVFQALSTCITSTQWRVDAAESDTGRRFVGIGREDGPAGIFGISRDNGVYNIVWCPAMMGRPRCGRAGILVENGVRLLALDGEAFPFEFVKA

Homology

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BLAST of Cla97C02G041770 vs. NCBI nr
Match: KAA0063690.1 (miraculin-like [Cucumis melo var. makuwa] >TYK18337.1 miraculin-like [Cucumis melo var. makuwa])

HSP 1 Score: 370.2 bits (949), Expect = 1.1e-98
Identity = 178/195 (91.28%), Postives = 183/195 (93.85%), Query Frame = 0


Query: 1   MMMLHKSLAIFGYACLFMAITSIAQFPPVFDTDGQPLRRGVEYYISPVFTDVGGNLTLKS 60
           MMMLHKSLAIFGYACLFMAITS AQ PPV DT+GQPLRRGVEYYISP  TDVGGNLTLKS
Sbjct: 1   MMMLHKSLAIFGYACLFMAITSTAQSPPVLDTNGQPLRRGVEYYISPAITDVGGNLTLKS 60

Query: 61  RSNAPCPLFVGQEPVTSTNIGLPVTFTPIMAGKDIIDEGTSLNIVFQALSTCITSTQWRV 120
           RSNAPCPLFVGQEPVTSTNIGLPVTF P  AGKDIIDEGTSLNIVF+ALSTC TSTQWRV
Sbjct: 61  RSNAPCPLFVGQEPVTSTNIGLPVTFRPTEAGKDIIDEGTSLNIVFEALSTCATSTQWRV 120

Query: 121 DAAESDTGRRFVGIGREDGPAGIFGISRDNGVYNIVWCPAMMGRPRCGRAGILVENGVRL 180
           DA ESDTGRRFVGIG EDGPAGIFGISRDNG YNIVWCPAMMGRPRCGRAGILVENGVRL
Sbjct: 121 DATESDTGRRFVGIGDEDGPAGIFGISRDNGAYNIVWCPAMMGRPRCGRAGILVENGVRL 180

Query: 181 LALDGEAFPFEFVKA 196
           +ALDG+AFPFEF+KA
Sbjct: 181 VALDGDAFPFEFIKA 195

[Close]

BLAST of Cla97C02G041770 vs. NCBI nr
Match: KGN60910.2 (hypothetical protein Csa_023447 [Cucumis sativus])

HSP 1 Score: 367.1 bits (941), Expect = 9.5e-98
Identity = 176/195 (90.26%), Postives = 182/195 (93.33%), Query Frame = 0


Query: 1   MMMLHKSLAIFGYACLFMAITSIAQFPPVFDTDGQPLRRGVEYYISPVFTDVGGNLTLKS 60
           MMMLHKSLAIFGYACLFMAITS AQ PPV DT+GQPL+RGVEYYISP  TDVGGNLTLKS
Sbjct: 170 MMMLHKSLAIFGYACLFMAITSTAQLPPVLDTNGQPLQRGVEYYISPAITDVGGNLTLKS 229

Query: 61  RSNAPCPLFVGQEPVTSTNIGLPVTFTPIMAGKDIIDEGTSLNIVFQALSTCITSTQWRV 120
           RSNAPCPLFVGQEPVTSTNIGLPVTF P   G DIIDEGTSLNIVFQALSTC+TSTQWRV
Sbjct: 230 RSNAPCPLFVGQEPVTSTNIGLPVTFRPTEEGIDIIDEGTSLNIVFQALSTCVTSTQWRV 289

Query: 121 DAAESDTGRRFVGIGREDGPAGIFGISRDNGVYNIVWCPAMMGRPRCGRAGILVENGVRL 180
           DA ESDTGRRFVGIG EDGPAGIFGISRDNG YNIVWCPAMMGRPRCGRAGILVENGVRL
Sbjct: 290 DATESDTGRRFVGIGDEDGPAGIFGISRDNGAYNIVWCPAMMGRPRCGRAGILVENGVRL 349

Query: 181 LALDGEAFPFEFVKA 196
           +ALDG+AFPFEF+KA
Sbjct: 350 VALDGDAFPFEFIKA 364

[Close]

BLAST of Cla97C02G041770 vs. NCBI nr
Match: KAA0063689.1 (miraculin-like [Cucumis melo var. makuwa] >TYK18338.1 miraculin-like [Cucumis melo var. makuwa])

HSP 1 Score: 367.1 bits (941), Expect = 9.5e-98
Identity = 176/193 (91.19%), Postives = 181/193 (93.78%), Query Frame = 0


Query: 3   MLHKSLAIFGYACLFMAITSIAQFPPVFDTDGQPLRRGVEYYISPVFTDVGGNLTLKSRS 62
           MLHKSLAIFGYACLFMAITS AQ PPV DT+GQPLRRGVEYYISP  TDVGGNLTLKSRS
Sbjct: 1   MLHKSLAIFGYACLFMAITSTAQSPPVLDTNGQPLRRGVEYYISPAITDVGGNLTLKSRS 60

Query: 63  NAPCPLFVGQEPVTSTNIGLPVTFTPIMAGKDIIDEGTSLNIVFQALSTCITSTQWRVDA 122
           NAPCPLFVGQEPVTSTNIGLPVTF P  AGKDIIDEGTSLNIVF+ALSTC TSTQWRVDA
Sbjct: 61  NAPCPLFVGQEPVTSTNIGLPVTFRPTEAGKDIIDEGTSLNIVFEALSTCATSTQWRVDA 120

Query: 123 AESDTGRRFVGIGREDGPAGIFGISRDNGVYNIVWCPAMMGRPRCGRAGILVENGVRLLA 182
            ESDTGRRFVGIG EDGPAGIFGISRDNG YNIVWCPAMMGRPRCGRAGILVENGVRL+A
Sbjct: 121 TESDTGRRFVGIGDEDGPAGIFGISRDNGAYNIVWCPAMMGRPRCGRAGILVENGVRLVA 180

Query: 183 LDGEAFPFEFVKA 196
           LDG+AFPFEF+KA
Sbjct: 181 LDGDAFPFEFIKA 193

[Close]

BLAST of Cla97C02G041770 vs. NCBI nr
Match: XP_004139192.1 (miraculin [Cucumis sativus])

HSP 1 Score: 367.1 bits (941), Expect = 9.5e-98
Identity = 176/195 (90.26%), Postives = 182/195 (93.33%), Query Frame = 0


Query: 1   MMMLHKSLAIFGYACLFMAITSIAQFPPVFDTDGQPLRRGVEYYISPVFTDVGGNLTLKS 60
           MMMLHKSLAIFGYACLFMAITS AQ PPV DT+GQPL+RGVEYYISP  TDVGGNLTLKS
Sbjct: 1   MMMLHKSLAIFGYACLFMAITSTAQLPPVLDTNGQPLQRGVEYYISPAITDVGGNLTLKS 60

Query: 61  RSNAPCPLFVGQEPVTSTNIGLPVTFTPIMAGKDIIDEGTSLNIVFQALSTCITSTQWRV 120
           RSNAPCPLFVGQEPVTSTNIGLPVTF P   G DIIDEGTSLNIVFQALSTC+TSTQWRV
Sbjct: 61  RSNAPCPLFVGQEPVTSTNIGLPVTFRPTEEGIDIIDEGTSLNIVFQALSTCVTSTQWRV 120

Query: 121 DAAESDTGRRFVGIGREDGPAGIFGISRDNGVYNIVWCPAMMGRPRCGRAGILVENGVRL 180
           DA ESDTGRRFVGIG EDGPAGIFGISRDNG YNIVWCPAMMGRPRCGRAGILVENGVRL
Sbjct: 121 DATESDTGRRFVGIGDEDGPAGIFGISRDNGAYNIVWCPAMMGRPRCGRAGILVENGVRL 180

Query: 181 LALDGEAFPFEFVKA 196
           +ALDG+AFPFEF+KA
Sbjct: 181 VALDGDAFPFEFIKA 195

[Close]

BLAST of Cla97C02G041770 vs. NCBI nr
Match: XP_038888526.1 (kunitz type trypsin inhibitor 106-like [Benincasa hispida])

HSP 1 Score: 360.5 bits (924), Expect = 8.9e-96
Identity = 172/195 (88.21%), Postives = 181/195 (92.82%), Query Frame = 0


Query: 1   MMMLHKSLAIFGYACLFMAITSIAQFPPVFDTDGQPLRRGVEYYISPVFTDVGGNLTLKS 60
           MMMLHKSLAIFGYACLFMAITS AQ PPV DTD QPLRRGVEYYI P  TDVGGNLTLKS
Sbjct: 1   MMMLHKSLAIFGYACLFMAITSTAQLPPVLDTDSQPLRRGVEYYIKPAITDVGGNLTLKS 60

Query: 61  RSNAPCPLFVGQEPVTSTNIGLPVTFTPIMAGKDIIDEGTSLNIVFQALSTCITSTQWRV 120
           RSNAPCPLFVGQEPVTSTNIGLPVTFTPI+AG+DII+E  SLNIVF+ALSTC+TSTQWRV
Sbjct: 61  RSNAPCPLFVGQEPVTSTNIGLPVTFTPIVAGEDIIEESRSLNIVFEALSTCVTSTQWRV 120

Query: 121 DAAESDTGRRFVGIGREDGPAGIFGISRDNGVYNIVWCPAMMGRPRCGRAGILVENGVRL 180
           D AESDTGRRFVGIG +DGP+GIF I R+NGVYNIVWCPAMMGRPRCG AGILVENGVRL
Sbjct: 121 DEAESDTGRRFVGIGNDDGPSGIFRIDRNNGVYNIVWCPAMMGRPRCGSAGILVENGVRL 180

Query: 181 LALDGEAFPFEFVKA 196
           LALDG+AFPFEFVKA
Sbjct: 181 LALDGDAFPFEFVKA 195

[Close]

BLAST of Cla97C02G041770 vs. ExPASy Swiss-Prot
Match: G7LCV1 (Kunitz type trypsin inhibitor 106 OS=Medicago truncatula OX=3880 GN=KPI106 PE=1 SV=1)

HSP 1 Score: 130.6 bits (327), Expect = 2.0e-29
Identity = 82/205 (40.00%), Postives = 109/205 (53.17%), Query Frame = 0


Query: 1   MMMLHKSLAIFGYACLFMAITSIAQFPPVFDTDGQPLRRGVEYYISPVFTDVGGNLTLKS 60
           M +  ++L I  + CLF+  T+IAQF  V DT G+P+    EY+I PV T+ GG  T+ S
Sbjct: 3   MRLSIRTLIILAHVCLFITTTTIAQF--VLDTVGEPVEGDEEYFIRPVITNKGGRSTMVS 62

Query: 61  RSNAPCPLFVGQEPVTSTNIGLPVTFTPIMAGKDIID--EGTSLNIVFQALSTCITSTQW 120
           R N  CPL VG E +T    GL V FTP     D  D      L I FQA S+C+ ST+W
Sbjct: 63  R-NESCPLHVGLE-LTGLGRGLVVKFTPFAPHHDFDDVRVNRDLRITFQASSSCVQSTEW 122

Query: 121 RVDAAESDTGRRFVGIGREDGPAGIFG------ISRDNGVYNIVWCPAMM---GRPRCGR 180
           R+   ++ +GRR +  G +    G +G       +   G+YNI WCP  +    +  CG 
Sbjct: 123 RLGEKDTKSGRRLIITGTDSATNGSYGNFFRIVETPLEGMYNIQWCPTEVCPSCKFECGT 182

Query: 181 AGILVENGVRLLALDGEAFPFEFVK 195
             +L ENG  LLALDG   P  F K
Sbjct: 183 VDMLNENGKILLALDGGPLPLVFQK 203

[Close]

BLAST of Cla97C02G041770 vs. ExPASy Swiss-Prot
Match: G7LCV7 (Kunitz type trypsin inhibitor 111 OS=Medicago truncatula OX=3880 GN=KPI111 PE=1 SV=1)

HSP 1 Score: 127.1 bits (318), Expect = 2.2e-28
Identity = 76/205 (37.07%), Postives = 111/205 (54.15%), Query Frame = 0


Query: 1   MMMLHKSLAIFGYACLFMAITSIAQFPPVFDTDGQPLRRGVEYYISPVFTDVGGNLTLKS 60
           M  +  ++ I     L +  TSIAQF  V DT G+P+    +Y+I P  T  GG+LTL +
Sbjct: 1   MSTISFTIFILANVWLLVVTTSIAQF--VIDTSGEPVENDEDYFIRPAITGNGGSLTLVT 60

Query: 61  RSNAPCPLFVGQEPVTSTNIGLPVTFTPIMAGK--DIIDEGTSLNIVFQALSTCITSTQW 120
           R++  CP  VG +P      G  V  +P ++ +  D +  G  L ++FQA ++C  ST+W
Sbjct: 61  RNS--CPFNVGLDP--DAPQGFAVLLSPFVSNREEDEVRLGRDLRVIFQAGTSCGQSTEW 120

Query: 121 RVDAAESDTGRRFVGIGREDGPAGIFG------ISRDNGVYNIVWCPAMM---GRPRCGR 180
           R+   ++ TGRRF+  GR+D   G +G       +   G++NI WCP  +    +  CG 
Sbjct: 121 RLGERDATTGRRFIITGRDDSTVGSYGNFFRIVQTPSRGIFNIQWCPTEVCPSCKFECGT 180

Query: 181 AGILVENGVRLLALDGEAFPFEFVK 195
            GI+ ENG  LLALDG A P  F K
Sbjct: 181 VGIVRENGKILLALDGSALPVAFQK 199

[Close]

BLAST of Cla97C02G041770 vs. ExPASy Swiss-Prot
Match: A0A072TH68 (Kunitz type trypsin inhibitor 104 OS=Medicago truncatula OX=3880 GN=KPI104 PE=1 SV=1)

HSP 1 Score: 126.3 bits (316), Expect = 3.7e-28
Identity = 81/204 (39.71%), Postives = 107/204 (52.45%), Query Frame = 0


Query: 3   MLHKSLAIF--GYACLFMAITSIAQFPPVFDTDGQPLRRGVEYYISPVFTDVGGNLTLKS 62
           M  +SL IF   +  L MA TSIAQF  V DT G+P+    EY+I P  T  GG  TL +
Sbjct: 1   MSTRSLTIFILAHVWLLMATTSIAQF--VIDTSGEPVEDDEEYFIRPAITGNGGGSTLVT 60

Query: 63  RSNAPCPLFVGQEPVTSTNIGLPVTFTPIMAGKDIIDE--GTSLNIVFQALSTCITSTQW 122
             N PCPL VG +    T +G+ V FTP     D  D      L + F   ++C  ST W
Sbjct: 61  -GNGPCPLHVGLDNTEGT-LGVAVKFTPFAPQHDDDDVRLNRDLRVTFLTSTSCGQSTDW 120

Query: 123 RVDAAESDTGRRFVGIGREDG---PAGIFGI--SRDNGVYNIVWCP---AMMGRPRCGRA 182
           R+   ++ +GRR +  GR++G       F I  ++  G YNI WCP       + +CG  
Sbjct: 121 RLGEKDATSGRRLIVTGRDNGAGSQGNFFRIVQTQTGGTYNIQWCPTEACPSCKVQCGTV 180

Query: 183 GILVENGVRLLALDGEAFPFEFVK 195
           G++ ENG  LLALDG+A P  F K
Sbjct: 181 GVIRENGKNLLALDGDALPVVFQK 200

[Close]

BLAST of Cla97C02G041770 vs. ExPASy Swiss-Prot
Match: P13087 (Miraculin OS=Synsepalum dulcificum OX=3743 PE=1 SV=3)

HSP 1 Score: 102.4 bits (254), Expect = 5.7e-21
Identity = 69/184 (37.50%), Postives = 90/184 (48.91%), Query Frame = 0


Query: 28  PVFDTDGQPLRRGVEYYISPVFTDVGGNLTLKSRSNAPCPLFVGQEPVTSTNIGL----P 87
           PV D DG+ LR G  YYI PV  D GG LT+   +  P   FV    V  T   +    P
Sbjct: 35  PVLDIDGEKLRTGTNYYIVPVLRDHGGGLTVS--ATTPNGTFVCPPRVVQTRKEVDHDRP 94

Query: 88  VTFTPIMAGKDIIDEGTSLNIVFQALSTC--ITSTQWRVDAAESDTGRRFVGIGREDGPA 147
           + F P    +D++   T LNI F A   C   +ST WR+D  +  TG+ FV IG   G  
Sbjct: 95  LAFFPENPKEDVVRVSTDLNINFSAFMPCRWTSSTVWRLDKYDESTGQYFVTIGGVKGNP 154

Query: 148 GIFGISR--------DNGVYNIVWCPAMMG--RPRCGRAGILV-ENGVRLLALDGEAFPF 195
           G   IS          +G Y +V+CP + G  + +CG  GI + + G R LAL  + F F
Sbjct: 155 GPETISSWFKIEEFCGSGFYKLVFCPTVCGSCKVKCGDVGIYIDQKGRRRLALSDKPFAF 214

[Close]

BLAST of Cla97C02G041770 vs. ExPASy Swiss-Prot
Match: P07596 (Alpha-amylase/subtilisin inhibitor OS=Hordeum vulgare OX=4513 PE=1 SV=2)

HSP 1 Score: 79.7 bits (195), Expect = 4.0e-14
Identity = 47/120 (39.17%), Postives = 62/120 (51.67%), Query Frame = 0


Query: 27  PPVFDTDGQPLRRGVEYYISPVFTDVGGNLTLKSRSNAPCPLFVGQEPVTSTNIGLPVTF 86
           PPV DTDG  LR    YY+       GG LT+       CPLFV Q+P    + G PV  
Sbjct: 26  PPVHDTDGHELRADANYYVLSANRAHGGGLTMAPGHGRHCPLFVSQDP-NGQHDGFPVRI 85

Query: 87  TP--IMAGKDIIDEGTSLNIVFQALSTCITSTQWRVDAAESDTGRRFVGIG--REDGPAG 143
           TP  +     II   T + I F+A +TC+ ST+W +D +E   GRR V  G  ++  P+G
Sbjct: 86  TPYGVAPSDKIIRLSTDVRISFRAYTTCLQSTEWHID-SELAAGRRHVITGPVKDPSPSG 143

[Close]

BLAST of Cla97C02G041770 vs. ExPASy TrEMBL
Match: A0A5D3D468 (Miraculin-like OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold456G00520 PE=3 SV=1)

HSP 1 Score: 370.2 bits (949), Expect = 5.5e-99
Identity = 178/195 (91.28%), Postives = 183/195 (93.85%), Query Frame = 0


Query: 1   MMMLHKSLAIFGYACLFMAITSIAQFPPVFDTDGQPLRRGVEYYISPVFTDVGGNLTLKS 60
           MMMLHKSLAIFGYACLFMAITS AQ PPV DT+GQPLRRGVEYYISP  TDVGGNLTLKS
Sbjct: 1   MMMLHKSLAIFGYACLFMAITSTAQSPPVLDTNGQPLRRGVEYYISPAITDVGGNLTLKS 60

Query: 61  RSNAPCPLFVGQEPVTSTNIGLPVTFTPIMAGKDIIDEGTSLNIVFQALSTCITSTQWRV 120
           RSNAPCPLFVGQEPVTSTNIGLPVTF P  AGKDIIDEGTSLNIVF+ALSTC TSTQWRV
Sbjct: 61  RSNAPCPLFVGQEPVTSTNIGLPVTFRPTEAGKDIIDEGTSLNIVFEALSTCATSTQWRV 120

Query: 121 DAAESDTGRRFVGIGREDGPAGIFGISRDNGVYNIVWCPAMMGRPRCGRAGILVENGVRL 180
           DA ESDTGRRFVGIG EDGPAGIFGISRDNG YNIVWCPAMMGRPRCGRAGILVENGVRL
Sbjct: 121 DATESDTGRRFVGIGDEDGPAGIFGISRDNGAYNIVWCPAMMGRPRCGRAGILVENGVRL 180

Query: 181 LALDGEAFPFEFVKA 196
           +ALDG+AFPFEF+KA
Sbjct: 181 VALDGDAFPFEFIKA 195

[Close]

BLAST of Cla97C02G041770 vs. ExPASy TrEMBL
Match: A0A5A7VDV8 (Miraculin-like OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold456G00530 PE=3 SV=1)

HSP 1 Score: 367.1 bits (941), Expect = 4.6e-98
Identity = 176/193 (91.19%), Postives = 181/193 (93.78%), Query Frame = 0


Query: 3   MLHKSLAIFGYACLFMAITSIAQFPPVFDTDGQPLRRGVEYYISPVFTDVGGNLTLKSRS 62
           MLHKSLAIFGYACLFMAITS AQ PPV DT+GQPLRRGVEYYISP  TDVGGNLTLKSRS
Sbjct: 1   MLHKSLAIFGYACLFMAITSTAQSPPVLDTNGQPLRRGVEYYISPAITDVGGNLTLKSRS 60

Query: 63  NAPCPLFVGQEPVTSTNIGLPVTFTPIMAGKDIIDEGTSLNIVFQALSTCITSTQWRVDA 122
           NAPCPLFVGQEPVTSTNIGLPVTF P  AGKDIIDEGTSLNIVF+ALSTC TSTQWRVDA
Sbjct: 61  NAPCPLFVGQEPVTSTNIGLPVTFRPTEAGKDIIDEGTSLNIVFEALSTCATSTQWRVDA 120

Query: 123 AESDTGRRFVGIGREDGPAGIFGISRDNGVYNIVWCPAMMGRPRCGRAGILVENGVRLLA 182
            ESDTGRRFVGIG EDGPAGIFGISRDNG YNIVWCPAMMGRPRCGRAGILVENGVRL+A
Sbjct: 121 TESDTGRRFVGIGDEDGPAGIFGISRDNGAYNIVWCPAMMGRPRCGRAGILVENGVRLVA 180

Query: 183 LDGEAFPFEFVKA 196
           LDG+AFPFEF+KA
Sbjct: 181 LDGDAFPFEFIKA 193

[Close]

BLAST of Cla97C02G041770 vs. ExPASy TrEMBL
Match: A0A0A0LIK2 (Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_2G021500 PE=3 SV=1)

HSP 1 Score: 334.7 bits (857), Expect = 2.5e-88
Identity = 159/178 (89.33%), Postives = 165/178 (92.70%), Query Frame = 0


Query: 18  MAITSIAQFPPVFDTDGQPLRRGVEYYISPVFTDVGGNLTLKSRSNAPCPLFVGQEPVTS 77
           MAITS AQ PPV DT+GQPL+RGVEYYISP  TDVGGNLTLKSRSNAPCPLFVGQEPVTS
Sbjct: 1   MAITSTAQLPPVLDTNGQPLQRGVEYYISPAITDVGGNLTLKSRSNAPCPLFVGQEPVTS 60

Query: 78  TNIGLPVTFTPIMAGKDIIDEGTSLNIVFQALSTCITSTQWRVDAAESDTGRRFVGIGRE 137
           TNIGLPVTF P   G DIIDEGTSLNIVFQALSTC+TSTQWRVDA ESDTGRRFVGIG E
Sbjct: 61  TNIGLPVTFRPTEEGIDIIDEGTSLNIVFQALSTCVTSTQWRVDATESDTGRRFVGIGDE 120

Query: 138 DGPAGIFGISRDNGVYNIVWCPAMMGRPRCGRAGILVENGVRLLALDGEAFPFEFVKA 196
           DGPAGIFGISRDNG YNIVWCPAMMGRPRCGRAGILVENGVRL+ALDG+AFPFEF+KA
Sbjct: 121 DGPAGIFGISRDNGAYNIVWCPAMMGRPRCGRAGILVENGVRLVALDGDAFPFEFIKA 178

[Close]

BLAST of Cla97C02G041770 vs. ExPASy TrEMBL
Match: A0A6J1G3G3 (miraculin-like OS=Cucurbita moschata OX=3662 GN=LOC111450419 PE=3 SV=1)

HSP 1 Score: 323.2 bits (827), Expect = 7.6e-85
Identity = 152/193 (78.76%), Postives = 166/193 (86.01%), Query Frame = 0


Query: 3   MLHKSLAIFGYACLFMAITSIAQFPPVFDTDGQPLRRGVEYYISPVFTDVGGNLTLKSRS 62
           MLH SLAIF +  +FMAITS AQ PPV DTDGQPLRRGVEYYI P  TDV GNLTLKSRS
Sbjct: 1   MLHNSLAIFSFLLVFMAITSTAQLPPVLDTDGQPLRRGVEYYIKPAITDVAGNLTLKSRS 60

Query: 63  NAPCPLFVGQEPVTSTNIGLPVTFTPIMAGKDIIDEGTSLNIVFQALSTCITSTQWRVDA 122
           NAPCPL+VGQEP+ STNIGLPVTFTPI+AG+DII+E    N+VFQA STCITSTQWRVD 
Sbjct: 61  NAPCPLYVGQEPIASTNIGLPVTFTPIVAGEDIIEESMGFNVVFQASSTCITSTQWRVDE 120

Query: 123 AESDTGRRFVGIGREDGPAGIFGISRDNGVYNIVWCPAMMGRPRCGRAGILVENGVRLLA 182
            ES TGRRFVG+G ++GP GIF I R+NGVYNIVWCP M+GRPRCG AGILVE+GVRLLA
Sbjct: 121 MESATGRRFVGVGNDNGPTGIFRIDRNNGVYNIVWCPEMVGRPRCGSAGILVEDGVRLLA 180

Query: 183 LDGEAFPFEFVKA 196
           LDG AFPFEFVKA
Sbjct: 181 LDGNAFPFEFVKA 193

[Close]

BLAST of Cla97C02G041770 vs. ExPASy TrEMBL
Match: A0A6J1KC85 (miraculin-like OS=Cucurbita maxima OX=3661 GN=LOC111493610 PE=3 SV=1)

HSP 1 Score: 319.3 bits (817), Expect = 1.1e-83
Identity = 150/193 (77.72%), Postives = 165/193 (85.49%), Query Frame = 0


Query: 3   MLHKSLAIFGYACLFMAITSIAQFPPVFDTDGQPLRRGVEYYISPVFTDVGGNLTLKSRS 62
           MLH SLAIF Y  +F+AITS AQ PPV DTDGQPLRRGVEYYI P  T+V GNLTLK+RS
Sbjct: 1   MLHNSLAIFSYLLVFLAITSTAQLPPVLDTDGQPLRRGVEYYIKPAITNVAGNLTLKNRS 60

Query: 63  NAPCPLFVGQEPVTSTNIGLPVTFTPIMAGKDIIDEGTSLNIVFQALSTCITSTQWRVDA 122
           NAPCPL+VGQEPV STNIGLPVTFTPI+AG+DII+E    N+ FQA STCITSTQWRVD 
Sbjct: 61  NAPCPLYVGQEPVASTNIGLPVTFTPIVAGEDIIEESMGFNVAFQASSTCITSTQWRVDE 120

Query: 123 AESDTGRRFVGIGREDGPAGIFGISRDNGVYNIVWCPAMMGRPRCGRAGILVENGVRLLA 182
            ES TGRRFVG+G ++GP GIF I R+NGVYNIVWCP M+GRPRCG AGILVE+GVRLLA
Sbjct: 121 MESATGRRFVGVGNDNGPTGIFRIDRNNGVYNIVWCPEMVGRPRCGSAGILVEDGVRLLA 180

Query: 183 LDGEAFPFEFVKA 196
           LDG AFPFEFVKA
Sbjct: 181 LDGNAFPFEFVKA 193

[Close]

BLAST of Cla97C02G041770 vs. TAIR 10
Match: AT1G17860.1 (Kunitz family trypsin and protease inhibitor protein )

HSP 1 Score: 70.1 bits (170), Expect = 2.2e-12
Identity = 59/199 (29.65%), Postives = 86/199 (43.22%), Query Frame = 0


Query: 8   LAIFGYACLFMA---ITSIAQFPPVFDTDGQPLRRGVEYYISPVFTDVGGNLTLKSRSNA 67
           L IF    +F++   +T+ A   PV D +G+ L  GV YYI PV    GG LT+ +    
Sbjct: 5   LYIFLLLAVFISHRGVTTEAAVEPVKDINGKSLLTGVNYYILPVIRGRGGGLTMSNLKTE 64

Query: 68  PCPLFVGQEPVTSTNIGLPVTFTPIMAGKDIIDEGTSLNIVFQALSTCITSTQWRVDAAE 127
            CP  V Q+     + GLPV F+P    +  I   T +NI F   S       W +   +
Sbjct: 65  TCPTSVIQDQF-EVSQGLPVKFSPYDKSR-TIPVSTDVNIKFSPTSI------WELANFD 124

Query: 128 SDTGRRFVGIGREDGPAG------IFGISRDNGVYNIVWCPAMMG--RPRCGRAGILVEN 187
             T + F+     +G  G       F I +    Y I +CP +    +  C   G+ V++
Sbjct: 125 ETTKQWFISTCGVEGNPGQKTVDNWFKIDKFEKDYKIRFCPTVCNFCKVICRDVGVFVQD 184

Query: 188 GVRLLALDGEAFPFEFVKA 196
           G R LAL        F +A
Sbjct: 185 GKRRLALSDVPLKVMFKRA 195

The following BLAST results are available for this feature:

BLAST of Cla97C02G041770 vs. NCBI nr


Match Name	E-value	Identity	Description
KAA0063690.1	1.1e-98	91.28	miraculin-like [Cucumis melo var. makuwa] >TYK18337.1 miraculin-like [Cucumis me...	[more]
KGN60910.2	9.5e-98	90.26	hypothetical protein Csa_023447 [Cucumis sativus]	[more]
KAA0063689.1	9.5e-98	91.19	miraculin-like [Cucumis melo var. makuwa] >TYK18338.1 miraculin-like [Cucumis me...	[more]
XP_004139192.1	9.5e-98	90.26	miraculin [Cucumis sativus]	[more]
XP_038888526.1	8.9e-96	88.21	kunitz type trypsin inhibitor 106-like [Benincasa hispida]	[more]

BLAST of Cla97C02G041770 vs. ExPASy Swiss-Prot


Match Name	E-value	Identity	Description
G7LCV1	2.0e-29	40.00	Kunitz type trypsin inhibitor 106 OS=Medicago truncatula OX=3880 GN=KPI106 PE=1 ...	[more]
G7LCV7	2.2e-28	37.07	Kunitz type trypsin inhibitor 111 OS=Medicago truncatula OX=3880 GN=KPI111 PE=1 ...	[more]
A0A072TH68	3.7e-28	39.71	Kunitz type trypsin inhibitor 104 OS=Medicago truncatula OX=3880 GN=KPI104 PE=1 ...	[more]
P13087	5.7e-21	37.50	Miraculin OS=Synsepalum dulcificum OX=3743 PE=1 SV=3	[more]
P07596	4.0e-14	39.17	Alpha-amylase/subtilisin inhibitor OS=Hordeum vulgare OX=4513 PE=1 SV=2	[more]

BLAST of Cla97C02G041770 vs. ExPASy TrEMBL


Match Name	E-value	Identity	Description
A0A5D3D468	5.5e-99	91.28	Miraculin-like OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold456G00520...	[more]
A0A5A7VDV8	4.6e-98	91.19	Miraculin-like OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold456G00530...	[more]
A0A0A0LIK2	2.5e-88	89.33	Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_2G021500 PE=3 SV=1	[more]
A0A6J1G3G3	7.6e-85	78.76	miraculin-like OS=Cucurbita moschata OX=3662 GN=LOC111450419 PE=3 SV=1	[more]
A0A6J1KC85	1.1e-83	77.72	miraculin-like OS=Cucurbita maxima OX=3661 GN=LOC111493610 PE=3 SV=1	[more]

BLAST of Cla97C02G041770 vs. TAIR 10


Match Name	E-value	Identity	Description
AT1G17860.1	2.2e-12	29.65	Kunitz family trypsin and protease inhibitor protein	[more]

InterPro

Analysis Name: InterPro Annotations of Watermelon (97103) v2.5
Date Performed: 2022-01-31


IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
IPR002160	Proteinase inhibitor I3, Kunitz legume	SMART	SM00452	kul_2	coord: 28..195 e-value: 1.2E-29 score: 114.5
IPR002160	Proteinase inhibitor I3, Kunitz legume	PFAM	PF00197	Kunitz_legume	coord: 29..194 e-value: 1.3E-40 score: 139.2
IPR002160	Proteinase inhibitor I3, Kunitz legume	PANTHER	PTHR33107	KUNITZ TRYPSIN INHIBITOR 2	coord: 4..194
IPR002160	Proteinase inhibitor I3, Kunitz legume	PROSITE	PS00283	SOYBEAN_KUNITZ	coord: 29..45
IPR002160	Proteinase inhibitor I3, Kunitz legume	CDD	cd00178	STI	coord: 28..194 e-value: 1.84838E-38 score: 128.229
None	No IPR available	GENE3D	2.80.10.50		coord: 24..195 e-value: 9.5E-47 score: 160.9
None	No IPR available	PANTHER	PTHR33107:SF51	PUTATIVE-RELATED	coord: 4..194
IPR011065	Kunitz inhibitor STI-like superfamily	SUPERFAMILY	50386	STI-like	coord: 27..195

Relationships

The following mRNA feature(s) are a part of this gene:


Feature Name	Unique Name	Type
Cla97C02G041770.2	Cla97C02G041770.2	mRNA

GO Annotation

GO Assignments

This gene is annotated with the following GO terms.

Category	Term Accession	Term Name
biological_process	GO:0006952	defense response
biological_process	GO:0010951	negative regulation of endopeptidase activity
biological_process	GO:0007165	signal transduction
molecular_function	GO:0043531	ADP binding
molecular_function	GO:0004866	endopeptidase inhibitor activity
molecular_function	GO:0003953	NAD+ nucleosidase activity