Cla97C02G040000 (gene) Watermelon (97103) v2.5

Overview
NameCla97C02G040000
Typegene
OrganismCitrullus lanatus subsp. vulgaris cv. 97103 (Watermelon (97103) v2.5)
DescriptionEukaryotic aspartyl protease family protein
LocationCla97Chr02: 27890920 .. 27893721 (-)
RNA-Seq ExpressionCla97C02G040000
SyntenyCla97C02G040000
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: polypeptideexonCDS
Hold the cursor over a type above to highlight its positions in the sequence below.
ATGTTCCTTCAACAACCCTCTTCTCTTCTCTTCTTTCTCCATCTCCTCTTCTTCTCCGCCGCGGTTGCCGCCCGTCCATCTCCTCCGACAAAGTTTCAATACCTTAATGTCAAAGCTACCAAATTGGACTTTAATGACGGTCAGATTCTTCATACCCTTAATTTCTCCGACGGCCACCGGCAAGCGTCCGGTCACAAATCCGACAATAATACATTCAAACTCAACCTTGTCCACCGGGATAAGCTTTCCCACGTCCATGGCCATCGTCATGGCTTTAATGAACGCATCAAAAGAGACGCCATAAGAGTCGCCACCCTCGTCCGCCGCCTATCTCACGGCGGCGGCGCCGTGCAAGATAACAAATACAAGGTGGCCAATTTTGGCACGGATGTGATTTCAGGCAAGGGAAATTTAGAATTTTTTTGAATACCCTTTAAAATACAATGTGTATATTCTTGAAATTTTAATTATTGTATTTATTTGGGAATTAGGAATGGAAGAGGGAAGCGGAGAATATTTTGTTCGGATCGGAGTTGGGAGCCCGCCGAGGAGTCAGTATATGGTGATTGATTCCGGCAGTGATATTGTGTGGGTTCAATGTCAACCTTGCACCCGATGCTACCAACAATCCGACCCGGTTTTCGACCCGGCTGACTCCTCCTCCTTCGCTGGTGTGTCTTGTGGCTCCGCCGTTTGCGACCGCCTTGAGAACACCGGCTGTAACGCTGGACGATGTCGGTATGAGGTGTCCTATGGGGACGGGTCGTACACTAAAGGCACTCTCGCTCTCGAAACTCTCACCCTTGGCCAAGTAACTACTATACTTATATTATATTTTTTATTATTTACTCATATTCATATTTTTAAATCCCTCTTTTTTCTTACCTTACCTTACCTTAGCTTAGCTTAGAAGGCTTAAATATTATTGTAGTAATCAACTAATTTGTTCAAATATGACAAATAGTATGTGTTATGCACTTCAAATTGAGCCAAGGGGAATTTAAAATATTTTTTTTCTAGAAAAAAAAAAAAGAAAGTTAGAGGTGTAATTGAACCTTTTTCTCCTTGTTCTAAAACTAGTTTCATGGTCTTTTTGACATGATCATCCAACCACTACAAAAGGAAAAAAGAAAAAAAAAATTATATGTAACATTGTCACGTGTTTTTTTCTTATCCAAATAGTAAGTTCTATTCTCAAGATTTTCACATAACCACGATTTTTTTAGAGAAGTTTATCATGACAACTTTTAAGTTTTAAGTAAAGATTTGTGTTAAAAAAAAATACTTACAACTTCTAATTTTTTAATTTGATATTTAATGGATATCTAAATTTTAGAAATGTATAAGTCTATTTTTTTTTATTATGTATTTATTAGATCTATAATGTAGTTGAAAAAAAAAATTAAATCAATTGATCTATATAAAAACAAGGAAAATGAAGTTAATTGTTTCAAGGTTTCTAAGCTTTTTAACTTTATGTTTAATAAATAAACTAATTTGAAAAAAAAATGTTAAATATGTATACAATCTATTAGATACAAAAGAAAAAAAAAATTAAAGCACCGTTTGGTTGCATTTGGTTTTTAGTTTTTTGAAATTATGTATAAAGACACTTTTTCCATTTCTAAAACTAAGTCATATTTTGAAAACTCAGAAGAGTAGTTAATAAAAACTTATTTTTGTTTTTATAATTTGACTAAGAATTTAATTCTTTTACTCAGGAGTAAGAAATTAAGAGAAAATAAACATAATTTTCAAAGACCAAATAGTTACCAAATAGAGCCTAATGAAGTATTAAATACAAAATTGATAGTTAAATATAATATATTTGACACTTTAGAACTTCAAAGTGTAGTGGATACTTATATATTCAACATCATGCAAATTTAATGACTCAACTGTTATAAACTTGTATCATTAACCTCGAAGTTAAATATTTGATTTCTTCACCACATGTTTGAAAAAAAAAAACAGAAATCGAAGAGAATTTTTTAAGATTGTGGGACTAAATGGCATGTAATTTAACCCTTATATTTTTATAATTTATAATTCATTCATTCATATTCTCTCAATAAGGTGATGATTCGTGACGTGGCAATCGGGTGCGGTCATACAAACCAAGGCATGTTTATAGGGGCCGCCGGGTTACTCGGACTCGGTGGAGGTTCAATGTCATTCATCGGCCAACTCGGTGGTCAAACCGGCGGCGCATTCAGCTACTGTTTAGTGAGCCGAGGCACCGGCTCGACCGGAACATTGGAATTCGGCCGTGGAACACTGCCAGTTGGCGCCACGTGGATCTCCCTAATTCGAAACCCACGCGCCCCAAGCTTCTACTACATAGGACTCGCCGGCATCGGGGTCGGCGGCGTTCGAGTTCCGGTACCAGAAGAAACTTTTCAACTCACCGAGTACGGAACAAACGGCGTCGTAATGGACACTGGCACCGCCGTGACGCGTCTACCAACGGCGGCCTACGTGGCACTCCGTGATTCGTTCACAGCTCAAACTAGCAACCTCCCACGAGCGCCTGGTGTTTCGATCTTCGACACGTGTTACGATCTCAACGGGTTCGAATCCGTACGAGTGCCCACGGTGTCGTTTTACTTCTCCGATGGACCCGTTCTGACGTTGCCAGCGAAGAACTTTTTGATTCCGGTGGACGGCGGGGGAACTTTTTGCCTGGCTTTTGCTCCGTCGCCGTCGGGACTTTCTATTATCGGAAACATCCAGCAGGAAGGAATTCAAATTTCATTCGATGGGGCTAATGGGTTCGTTGGATTTGGCCCAAATATTTGCTAA

mRNA sequence

ATGTTCCTTCAACAACCCTCTTCTCTTCTCTTCTTTCTCCATCTCCTCTTCTTCTCCGCCGCGGTTGCCGCCCGTCCATCTCCTCCGACAAAGTTTCAATACCTTAATGTCAAAGCTACCAAATTGGACTTTAATGACGGTCAGATTCTTCATACCCTTAATTTCTCCGACGGCCACCGGCAAGCGTCCGGTCACAAATCCGACAATAATACATTCAAACTCAACCTTGTCCACCGGGATAAGCTTTCCCACGTCCATGGCCATCGTCATGGCTTTAATGAACGCATCAAAAGAGACGCCATAAGAGTCGCCACCCTCGTCCGCCGCCTATCTCACGGCGGCGGCGCCGTGCAAGATAACAAATACAAGGTGGCCAATTTTGGCACGGATGTGATTTCAGGAATGGAAGAGGGAAGCGGAGAATATTTTGTTCGGATCGGAGTTGGGAGCCCGCCGAGGAGTCAGTATATGGTGATTGATTCCGGCAGTGATATTGTGTGGGTTCAATGTCAACCTTGCACCCGATGCTACCAACAATCCGACCCGGTTTTCGACCCGGCTGACTCCTCCTCCTTCGCTGGTGTGTCTTGTGGCTCCGCCGTTTGCGACCGCCTTGAGAACACCGGCTGTAACGCTGGACGATGTCGGTATGAGGTGTCCTATGGGGACGGGTCGTACACTAAAGGCACTCTCGCTCTCGAAACTCTCACCCTTGGCCAAGTGATGATTCGTGACGTGGCAATCGGGTGCGGTCATACAAACCAAGGCATGTTTATAGGGGCCGCCGGGTTACTCGGACTCGGTGGAGGTTCAATGTCATTCATCGGCCAACTCGGTGGTCAAACCGGCGGCGCATTCAGCTACTGTTTAGTGAGCCGAGGCACCGGCTCGACCGGAACATTGGAATTCGGCCGTGGAACACTGCCAGTTGGCGCCACGTGGATCTCCCTAATTCGAAACCCACGCGCCCCAAGCTTCTACTACATAGGACTCGCCGGCATCGGGGTCGGCGGCGTTCGAGTTCCGGTACCAGAAGAAACTTTTCAACTCACCGAGTACGGAACAAACGGCGTCGTAATGGACACTGGCACCGCCGTGACGCGTCTACCAACGGCGGCCTACGTGGCACTCCGTGATTCGTTCACAGCTCAAACTAGCAACCTCCCACGAGCGCCTGGTGTTTCGATCTTCGACACGTGTTACGATCTCAACGGGTTCGAATCCGTACGAGTGCCCACGGTGTCGTTTTACTTCTCCGATGGACCCGTTCTGACGTTGCCAGCGAAGAACTTTTTGATTCCGGTGGACGGCGGGGGAACTTTTTGCCTGGCTTTTGCTCCGTCGCCGTCGGGACTTTCTATTATCGGAAACATCCAGCAGGAAGGAATTCAAATTTCATTCGATGGGGCTAATGGGTTCGTTGGATTTGGCCCAAATATTTGCTAA

Coding sequence (CDS)

ATGTTCCTTCAACAACCCTCTTCTCTTCTCTTCTTTCTCCATCTCCTCTTCTTCTCCGCCGCGGTTGCCGCCCGTCCATCTCCTCCGACAAAGTTTCAATACCTTAATGTCAAAGCTACCAAATTGGACTTTAATGACGGTCAGATTCTTCATACCCTTAATTTCTCCGACGGCCACCGGCAAGCGTCCGGTCACAAATCCGACAATAATACATTCAAACTCAACCTTGTCCACCGGGATAAGCTTTCCCACGTCCATGGCCATCGTCATGGCTTTAATGAACGCATCAAAAGAGACGCCATAAGAGTCGCCACCCTCGTCCGCCGCCTATCTCACGGCGGCGGCGCCGTGCAAGATAACAAATACAAGGTGGCCAATTTTGGCACGGATGTGATTTCAGGAATGGAAGAGGGAAGCGGAGAATATTTTGTTCGGATCGGAGTTGGGAGCCCGCCGAGGAGTCAGTATATGGTGATTGATTCCGGCAGTGATATTGTGTGGGTTCAATGTCAACCTTGCACCCGATGCTACCAACAATCCGACCCGGTTTTCGACCCGGCTGACTCCTCCTCCTTCGCTGGTGTGTCTTGTGGCTCCGCCGTTTGCGACCGCCTTGAGAACACCGGCTGTAACGCTGGACGATGTCGGTATGAGGTGTCCTATGGGGACGGGTCGTACACTAAAGGCACTCTCGCTCTCGAAACTCTCACCCTTGGCCAAGTGATGATTCGTGACGTGGCAATCGGGTGCGGTCATACAAACCAAGGCATGTTTATAGGGGCCGCCGGGTTACTCGGACTCGGTGGAGGTTCAATGTCATTCATCGGCCAACTCGGTGGTCAAACCGGCGGCGCATTCAGCTACTGTTTAGTGAGCCGAGGCACCGGCTCGACCGGAACATTGGAATTCGGCCGTGGAACACTGCCAGTTGGCGCCACGTGGATCTCCCTAATTCGAAACCCACGCGCCCCAAGCTTCTACTACATAGGACTCGCCGGCATCGGGGTCGGCGGCGTTCGAGTTCCGGTACCAGAAGAAACTTTTCAACTCACCGAGTACGGAACAAACGGCGTCGTAATGGACACTGGCACCGCCGTGACGCGTCTACCAACGGCGGCCTACGTGGCACTCCGTGATTCGTTCACAGCTCAAACTAGCAACCTCCCACGAGCGCCTGGTGTTTCGATCTTCGACACGTGTTACGATCTCAACGGGTTCGAATCCGTACGAGTGCCCACGGTGTCGTTTTACTTCTCCGATGGACCCGTTCTGACGTTGCCAGCGAAGAACTTTTTGATTCCGGTGGACGGCGGGGGAACTTTTTGCCTGGCTTTTGCTCCGTCGCCGTCGGGACTTTCTATTATCGGAAACATCCAGCAGGAAGGAATTCAAATTTCATTCGATGGGGCTAATGGGTTCGTTGGATTTGGCCCAAATATTTGCTAA

Protein sequence

MFLQQPSSLLFFLHLLFFSAAVAARPSPPTKFQYLNVKATKLDFNDGQILHTLNFSDGHRQASGHKSDNNTFKLNLVHRDKLSHVHGHRHGFNERIKRDAIRVATLVRRLSHGGGAVQDNKYKVANFGTDVISGMEEGSGEYFVRIGVGSPPRSQYMVIDSGSDIVWVQCQPCTRCYQQSDPVFDPADSSSFAGVSCGSAVCDRLENTGCNAGRCRYEVSYGDGSYTKGTLALETLTLGQVMIRDVAIGCGHTNQGMFIGAAGLLGLGGGSMSFIGQLGGQTGGAFSYCLVSRGTGSTGTLEFGRGTLPVGATWISLIRNPRAPSFYYIGLAGIGVGGVRVPVPEETFQLTEYGTNGVVMDTGTAVTRLPTAAYVALRDSFTAQTSNLPRAPGVSIFDTCYDLNGFESVRVPTVSFYFSDGPVLTLPAKNFLIPVDGGGTFCLAFAPSPSGLSIIGNIQQEGIQISFDGANGFVGFGPNIC
Homology
BLAST of Cla97C02G040000 vs. NCBI nr
Match: XP_038893071.1 (protein ASPARTIC PROTEASE IN GUARD CELL 2-like [Benincasa hispida])

HSP 1 Score: 918.7 bits (2373), Expect = 2.1e-263
Identity = 456/481 (94.80%), Postives = 464/481 (96.47%), Query Frame = 0

Query: 1   MFLQQPSSLLFFLHLLFFSAAVAARPSPPTKFQYLNVKATKLDFNDGQILHTLNFSDGHR 60
           MFL+QPSSLLFFL  LFFSA  AA  S PTKFQYLNVKATKLDFND QILHTLNFS   R
Sbjct: 1   MFLKQPSSLLFFLFFLFFSA--AAGRSSPTKFQYLNVKATKLDFNDDQILHTLNFSHARR 60

Query: 61  QASGHKSDNNTFKLNLVHRDKLSHVHGHRHGFNERIKRDAIRVATLVRRLSHGGGAVQDN 120
           Q SGHKSDNNTFKLNL+HRDKLSHVHGHRHGFNERIKRDAIRVATLVRRLSHGGGAVQDN
Sbjct: 61  QVSGHKSDNNTFKLNLLHRDKLSHVHGHRHGFNERIKRDAIRVATLVRRLSHGGGAVQDN 120

Query: 121 KYKVANFGTDVISGMEEGSGEYFVRIGVGSPPRSQYMVIDSGSDIVWVQCQPCTRCYQQS 180
           KYKVA+F TDVISGMEEGSGEYFVRIGVGSPPRSQYMVIDSGSDIVWVQC+PC+RCYQQS
Sbjct: 121 KYKVADFATDVISGMEEGSGEYFVRIGVGSPPRSQYMVIDSGSDIVWVQCKPCSRCYQQS 180

Query: 181 DPVFDPADSSSFAGVSCGSAVCDRLENTGCNAGRCRYEVSYGDGSYTKGTLALETLTLGQ 240
           DPVFDPADSSSFAGVSC S VCDRLENTGCNAGRCRYEVSYGDGSYTKGTLALETLT+G+
Sbjct: 181 DPVFDPADSSSFAGVSCDSIVCDRLENTGCNAGRCRYEVSYGDGSYTKGTLALETLTVGE 240

Query: 241 VMIRDVAIGCGHTNQGMFIGAAGLLGLGGGSMSFIGQLGGQTGGAFSYCLVSRGTGSTGT 300
           VMIRDVAIGCGHTNQGMFIGAAGLLGLGGGSMSFIGQLGGQTGGAFSYCLVSRGTGSTGT
Sbjct: 241 VMIRDVAIGCGHTNQGMFIGAAGLLGLGGGSMSFIGQLGGQTGGAFSYCLVSRGTGSTGT 300

Query: 301 LEFGRGTLPVGATWISLIRNPRAPSFYYIGLAGIGVGGVRVPVPEETFQLTEYGTNGVVM 360
           LEFGRG LPVGATWISLIRNPRAPSFYYIGLAGIGVGGVRV VPEETFQLTEYGTNGVVM
Sbjct: 301 LEFGRGALPVGATWISLIRNPRAPSFYYIGLAGIGVGGVRVSVPEETFQLTEYGTNGVVM 360

Query: 361 DTGTAVTRLPTAAYVALRDSFTAQTSNLPRAPGVSIFDTCYDLNGFESVRVPTVSFYFSD 420
           DTGTAVTRLPTAAYVALRDSFTAQTSNLPRAPGVSIFDTCYDLNGFESVRVPTVSFYFSD
Sbjct: 361 DTGTAVTRLPTAAYVALRDSFTAQTSNLPRAPGVSIFDTCYDLNGFESVRVPTVSFYFSD 420

Query: 421 GPVLTLPAKNFLIPVDGGGTFCLAFAPSPSGLSIIGNIQQEGIQISFDGANGFVGFGPNI 480
           GPVLTLPAKNFLIPVDGGGTFCLAFAPSPSGLSIIGNIQQEGIQISFDGANGFVGFGPN+
Sbjct: 421 GPVLTLPAKNFLIPVDGGGTFCLAFAPSPSGLSIIGNIQQEGIQISFDGANGFVGFGPNV 479

Query: 481 C 482
           C
Sbjct: 481 C 479

BLAST of Cla97C02G040000 vs. NCBI nr
Match: XP_008445900.1 (PREDICTED: protein ASPARTIC PROTEASE IN GUARD CELL 2-like [Cucumis melo])

HSP 1 Score: 899.4 bits (2323), Expect = 1.3e-257
Identity = 445/480 (92.71%), Postives = 460/480 (95.83%), Query Frame = 0

Query: 2   FLQQPSSLLFFLHLLFFSAAVAARPSPPTKFQYLNVKATKLDFNDGQILHTLNFSDGHRQ 61
           F Q  SSLLFFL +L   AA AARPS PTKFQYLNVKATKLDFNDGQILHTLNFSD HRQ
Sbjct: 3   FKQLISSLLFFLAVLVVVAA-AARPSSPTKFQYLNVKATKLDFNDGQILHTLNFSDAHRQ 62

Query: 62  ASGHKSDNNTFKLNLVHRDKLSHVHGHRHGFNERIKRDAIRVATLVRRLSHGGGAVQDNK 121
            SGHKS N+TFKLNL+HRDKLSHVHGHR GFN+R+KRDAIRVATLVRRLSHG  AV+D++
Sbjct: 63  VSGHKSHNDTFKLNLLHRDKLSHVHGHRRGFNDRMKRDAIRVATLVRRLSHGAAAVEDSR 122

Query: 122 YKVANFGTDVISGMEEGSGEYFVRIGVGSPPRSQYMVIDSGSDIVWVQCQPCTRCYQQSD 181
           +KVANF TDVISGMEEGSGEYFVRIGVGSPPRSQYMVIDSGSDIVWVQC+PC+RCYQQSD
Sbjct: 123 FKVANFATDVISGMEEGSGEYFVRIGVGSPPRSQYMVIDSGSDIVWVQCKPCSRCYQQSD 182

Query: 182 PVFDPADSSSFAGVSCGSAVCDRLENTGCNAGRCRYEVSYGDGSYTKGTLALETLTLGQV 241
           PVFDPADSSSFAGVSCGS VCDRLENTGCNAGRCRYEVSYGDGSYTKGTLALETLT+GQV
Sbjct: 183 PVFDPADSSSFAGVSCGSDVCDRLENTGCNAGRCRYEVSYGDGSYTKGTLALETLTVGQV 242

Query: 242 MIRDVAIGCGHTNQGMFIGAAGLLGLGGGSMSFIGQLGGQTGGAFSYCLVSRGTGSTGTL 301
           MIRDVAIGCGHTNQGMFIGAAGLLGLGGGSMSFIGQLGGQTGGAFSYCLVSRGTGSTGTL
Sbjct: 243 MIRDVAIGCGHTNQGMFIGAAGLLGLGGGSMSFIGQLGGQTGGAFSYCLVSRGTGSTGTL 302

Query: 302 EFGRGTLPVGATWISLIRNPRAPSFYYIGLAGIGVGGVRVPVPEETFQLTEYGTNGVVMD 361
           EFGRG LPVGATWISLIRNPRAPSFYYIGLAGIGVGGVRV +PEETFQLTE+GTNGVVMD
Sbjct: 303 EFGRGALPVGATWISLIRNPRAPSFYYIGLAGIGVGGVRVSIPEETFQLTEFGTNGVVMD 362

Query: 362 TGTAVTRLPTAAYVALRDSFTAQTSNLPRAPGVSIFDTCYDLNGFESVRVPTVSFYFSDG 421
           TGTAVTRLPT+AYVALRDSFTAQTSNLPRAPGVSIFDTCYDLNGFESVRVPTVSFYFSDG
Sbjct: 363 TGTAVTRLPTSAYVALRDSFTAQTSNLPRAPGVSIFDTCYDLNGFESVRVPTVSFYFSDG 422

Query: 422 PVLTLPAKNFLIPVDGGGTFCLAFAPSPSGLSIIGNIQQEGIQISFDGANGFVGFGPNIC 481
           P LTLPAKNFLIPVDGGGTFCLAFAPSPSGLSIIGNIQQEGIQISFDGANGFVGFGPNIC
Sbjct: 423 PALTLPAKNFLIPVDGGGTFCLAFAPSPSGLSIIGNIQQEGIQISFDGANGFVGFGPNIC 481

BLAST of Cla97C02G040000 vs. NCBI nr
Match: XP_004147103.1 (protein ASPARTIC PROTEASE IN GUARD CELL 2 [Cucumis sativus] >KGN51568.1 hypothetical protein Csa_008597 [Cucumis sativus])

HSP 1 Score: 887.5 bits (2292), Expect = 5.2e-254
Identity = 440/482 (91.29%), Postives = 455/482 (94.40%), Query Frame = 0

Query: 1   MFLQQPSSLLFFLHLLFFSAAVAARPSPPTKFQYLNVKATKLDFNDGQILHTLNFSDGHR 60
           MF +Q  S L FL ++   AA AAR S  TKFQYLNVKATKLDFNDGQILH LNFSDGHR
Sbjct: 1   MFFKQLFSSLLFLLVVIVGAAAAARLSSSTKFQYLNVKATKLDFNDGQILHALNFSDGHR 60

Query: 61  QASGHKSDNNTFKLNLVHRDKLSHVHGHRHGFNERIKRDAIRVATLVRRLSHGG-GAVQD 120
           Q SG+KSDNNTFKLNL+HRDKLSHVHGHR GFN+R+KRDAIRVATLVRRLSHG   AV+D
Sbjct: 61  QVSGYKSDNNTFKLNLLHRDKLSHVHGHRRGFNDRMKRDAIRVATLVRRLSHGAPAAVKD 120

Query: 121 NKYKVANFGTDVISGMEEGSGEYFVRIGVGSPPRSQYMVIDSGSDIVWVQCQPCTRCYQQ 180
           ++YKVANF TDVISGME GSGEYFVRIGVGSPPR+QYMVIDSGSDIVWVQC+PC+RCYQQ
Sbjct: 121 SRYKVANFATDVISGMEAGSGEYFVRIGVGSPPRNQYMVIDSGSDIVWVQCKPCSRCYQQ 180

Query: 181 SDPVFDPADSSSFAGVSCGSAVCDRLENTGCNAGRCRYEVSYGDGSYTKGTLALETLTLG 240
           SDPVFDPADSSSFAGVSCGS VCDRLENTGCNAGRCRYEVSYGDGSYTKGTLALETLT+G
Sbjct: 181 SDPVFDPADSSSFAGVSCGSDVCDRLENTGCNAGRCRYEVSYGDGSYTKGTLALETLTVG 240

Query: 241 QVMIRDVAIGCGHTNQGMFIGAAGLLGLGGGSMSFIGQLGGQTGGAFSYCLVSRGTGSTG 300
           QVMIRDVAIGCGHTNQGMFIGAAGLLGLGGGSMSFIGQLGGQTGGAFSYCLVSRGTGSTG
Sbjct: 241 QVMIRDVAIGCGHTNQGMFIGAAGLLGLGGGSMSFIGQLGGQTGGAFSYCLVSRGTGSTG 300

Query: 301 TLEFGRGTLPVGATWISLIRNPRAPSFYYIGLAGIGVGGVRVPVPEETFQLTEYGTNGVV 360
            LEFGRG LPVGATWISLIRNPRAPSFYYIGLAGIGVGGVRV VPEETFQLTEYGTNGVV
Sbjct: 301 ALEFGRGALPVGATWISLIRNPRAPSFYYIGLAGIGVGGVRVSVPEETFQLTEYGTNGVV 360

Query: 361 MDTGTAVTRLPTAAYVALRDSFTAQTSNLPRAPGVSIFDTCYDLNGFESVRVPTVSFYFS 420
           MDTGTAVTR PTAAYVA RDSFTAQTSNLPRAPGVSIFDTCYDLNGFESVRVPTVSFYFS
Sbjct: 361 MDTGTAVTRFPTAAYVAFRDSFTAQTSNLPRAPGVSIFDTCYDLNGFESVRVPTVSFYFS 420

Query: 421 DGPVLTLPAKNFLIPVDGGGTFCLAFAPSPSGLSIIGNIQQEGIQISFDGANGFVGFGPN 480
           DGPVLTLPA+NFLIPVDGGGTFCLAFAPSPSGLSIIGNIQQEGIQISFDGANGFVGFGPN
Sbjct: 421 DGPVLTLPARNFLIPVDGGGTFCLAFAPSPSGLSIIGNIQQEGIQISFDGANGFVGFGPN 480

Query: 481 IC 482
           IC
Sbjct: 481 IC 482

BLAST of Cla97C02G040000 vs. NCBI nr
Match: KAA0034086.1 (protein ASPARTIC PROTEASE IN GUARD CELL 2-like [Cucumis melo var. makuwa])

HSP 1 Score: 864.8 bits (2233), Expect = 3.6e-247
Identity = 432/480 (90.00%), Postives = 447/480 (93.12%), Query Frame = 0

Query: 2   FLQQPSSLLFFLHLLFFSAAVAARPSPPTKFQYLNVKATKLDFNDGQILHTLNFSDGHRQ 61
           F Q  SSLLFFL +L   AA AARPS PTKFQYLNVKATKLDFNDGQILHTLNFSD HRQ
Sbjct: 3   FKQLISSLLFFLAVLVVVAA-AARPSSPTKFQYLNVKATKLDFNDGQILHTLNFSDAHRQ 62

Query: 62  ASGHKSDNNTFKLNLVHRDKLSHVHGHRHGFNERIKRDAIRVATLVRRLSHGGGAVQDNK 121
            SGHKS N+TFKLNL+HRDKLSHVHGHR GFN+R+KRDAIRVATLVRRLSHG  AV+D++
Sbjct: 63  VSGHKSHNDTFKLNLLHRDKLSHVHGHRRGFNDRMKRDAIRVATLVRRLSHGAAAVEDSR 122

Query: 122 YKVANFGTDVISGMEEGSGEYFVRIGVGSPPRSQYMVIDSGSDIVWVQCQPCTRCYQQSD 181
           +K              GSGEYFVRIGVGSPPRSQYMVIDSGSDIVWVQC+PC+RCYQQSD
Sbjct: 123 FK--------------GSGEYFVRIGVGSPPRSQYMVIDSGSDIVWVQCKPCSRCYQQSD 182

Query: 182 PVFDPADSSSFAGVSCGSAVCDRLENTGCNAGRCRYEVSYGDGSYTKGTLALETLTLGQV 241
           PVFDPADSSSFAGVSCGS VCDRLENTGCNAGRCRYEVSYGDGSYTKGTLALETLT+GQV
Sbjct: 183 PVFDPADSSSFAGVSCGSDVCDRLENTGCNAGRCRYEVSYGDGSYTKGTLALETLTVGQV 242

Query: 242 MIRDVAIGCGHTNQGMFIGAAGLLGLGGGSMSFIGQLGGQTGGAFSYCLVSRGTGSTGTL 301
           MIRDVAIGCGHTNQGMFIGAAGLLGLGGGSMSFIGQLGGQTGGAFSYCLVSRGTGSTGTL
Sbjct: 243 MIRDVAIGCGHTNQGMFIGAAGLLGLGGGSMSFIGQLGGQTGGAFSYCLVSRGTGSTGTL 302

Query: 302 EFGRGTLPVGATWISLIRNPRAPSFYYIGLAGIGVGGVRVPVPEETFQLTEYGTNGVVMD 361
           EFGRG LPVGATWISLIRNPRAPSFYYIGLAGIGVGGVRV +PEETFQLTE+GTNGVVMD
Sbjct: 303 EFGRGALPVGATWISLIRNPRAPSFYYIGLAGIGVGGVRVSIPEETFQLTEFGTNGVVMD 362

Query: 362 TGTAVTRLPTAAYVALRDSFTAQTSNLPRAPGVSIFDTCYDLNGFESVRVPTVSFYFSDG 421
           TGTAVTRLPT+AYVALRDSFTAQTSNLPRAPGVSIFDTCYDLNGFESVRVPTVSFYFSDG
Sbjct: 363 TGTAVTRLPTSAYVALRDSFTAQTSNLPRAPGVSIFDTCYDLNGFESVRVPTVSFYFSDG 422

Query: 422 PVLTLPAKNFLIPVDGGGTFCLAFAPSPSGLSIIGNIQQEGIQISFDGANGFVGFGPNIC 481
           P LTLPAKNFLIPVDGGGTFCLAFAPSPSGLSIIGNIQQEGIQISFDGANGFVGFGPNIC
Sbjct: 423 PALTLPAKNFLIPVDGGGTFCLAFAPSPSGLSIIGNIQQEGIQISFDGANGFVGFGPNIC 467

BLAST of Cla97C02G040000 vs. NCBI nr
Match: XP_023547721.1 (protein ASPARTIC PROTEASE IN GUARD CELL 2-like [Cucurbita pepo subsp. pepo])

HSP 1 Score: 840.1 bits (2169), Expect = 9.5e-240
Identity = 416/485 (85.77%), Postives = 440/485 (90.72%), Query Frame = 0

Query: 1   MFLQQPSS-LLFFLHLLFFSAAVA--ARPSPPTKFQYLNVKATKLDFNDGQILHTLNFSD 60
           MFL+ PSS LL  LHLL F AA A  ARPSPP  F YLNVK TKL+ N   IL TLNFSD
Sbjct: 1   MFLKHPSSPLLLLLHLLLFLAAAAALARPSPPPNFLYLNVKPTKLELNHTHILPTLNFSD 60

Query: 61  GHRQASGHKSDNNTFKLNLVHRDKLSHVHGHRHGFNERIKRDAIRVATLVRRLSH-GGGA 120
            H Q +  K  N T KLNL+HRDKL HVH HRH FNERIKRDAIRVATL+RRLSH    A
Sbjct: 61  AHLQVTAQKFHNTTLKLNLLHRDKLPHVHAHRHAFNERIKRDAIRVATLIRRLSHAAAAA 120

Query: 121 VQDNKYKVANFGTDVISGMEEGSGEYFVRIGVGSPPRSQYMVIDSGSDIVWVQCQPCTRC 180
            QDN YKVANF TDV+SGMEEGSGE+FVR+GVGSPPRSQYMVIDSGSDIVWVQCQPCT+C
Sbjct: 121 AQDNSYKVANFATDVVSGMEEGSGEFFVRVGVGSPPRSQYMVIDSGSDIVWVQCQPCTQC 180

Query: 181 YQQSDPVFDPADSSSFAGVSCGSAVCDRLENTGCNAGRCRYEVSYGDGSYTKGTLALETL 240
           YQQSDPVF+PADSSSFAGVSCGSAVCDRL+NT C+AGRCRYEVSYGDGSYTKGTLALET+
Sbjct: 181 YQQSDPVFNPADSSSFAGVSCGSAVCDRLDNTDCHAGRCRYEVSYGDGSYTKGTLALETI 240

Query: 241 TLGQVMIRDVAIGCGHTNQGMFIGAAGLLGLGGGSMSFIGQLGGQTGGAFSYCLVSRGTG 300
           T+GQVMIRDVAIGCGHTNQGMFIGAAGLLGLGGGSMSFIGQLGGQTGGAFSYCLVSR TG
Sbjct: 241 TMGQVMIRDVAIGCGHTNQGMFIGAAGLLGLGGGSMSFIGQLGGQTGGAFSYCLVSRDTG 300

Query: 301 STGTLEFGRGTLPVGATWISLIRNPRAPSFYYIGLAGIGVGGVRVPVPEETFQLTEYGTN 360
           STGTLEFGRG +PVGATWISLIRNPRAPSFYYIGLAG+GVGGVRVP+PEETFQL+EYGTN
Sbjct: 301 STGTLEFGRGAMPVGATWISLIRNPRAPSFYYIGLAGVGVGGVRVPIPEETFQLSEYGTN 360

Query: 361 GVVMDTGTAVTRLPTAAYVALRDSFTAQTSNLPRAPGVSIFDTCYDLNGFESVRVPTVSF 420
           GVVMDTGTAVTRLPTAAY A RD+FTAQT+NLPRA GVSIFDTC+DLNGFES+RVPTVSF
Sbjct: 361 GVVMDTGTAVTRLPTAAYEAFRDAFTAQTNNLPRAGGVSIFDTCFDLNGFESIRVPTVSF 420

Query: 421 YFSDGPVLTLPAKNFLIPVDGGGTFCLAFAPSPSGLSIIGNIQQEGIQISFDGANGFVGF 480
           YFSDGPVLTLPAKNFLIPV+G GTFCLAFAPSPSGLSIIGNIQQEGIQIS DGANGFVGF
Sbjct: 421 YFSDGPVLTLPAKNFLIPVNGAGTFCLAFAPSPSGLSIIGNIQQEGIQISIDGANGFVGF 480

Query: 481 GPNIC 482
           GPN+C
Sbjct: 481 GPNVC 485

BLAST of Cla97C02G040000 vs. ExPASy Swiss-Prot
Match: Q9LHE3 (Protein ASPARTIC PROTEASE IN GUARD CELL 2 OS=Arabidopsis thaliana OX=3702 GN=ASPG2 PE=2 SV=1)

HSP 1 Score: 638.3 bits (1645), Expect = 7.2e-182
Identity = 314/478 (65.69%), Postives = 378/478 (79.08%), Query Frame = 0

Query: 11  FFLHLLFFSAAVAARPSPPTKF-----QYLNVKATKLDFNDGQILHTLNFSDGHRQASGH 70
           FFLHL    ++ ++   P  +        L V AT  DFN+       +FSD        
Sbjct: 8   FFLHLHLHLSSSSSISFPDFQIIDVLQPPLTVTATLPDFNN------THFSD-------- 67

Query: 71  KSDNNTFKLNLVHRDKLSHV--HGHRHGFNERIKRDAIRVATLVRRLSHGGGAVQDNKYK 130
              ++ + L L+HRD+   V    H H  + R++RD  RV+ ++RR+S       D++Y+
Sbjct: 68  -ESSSKYTLRLLHRDRFPSVTYRNHHHRLHARMRRDTDRVSAILRRISGKVIPSSDSRYE 127

Query: 131 VANFGTDVISGMEEGSGEYFVRIGVGSPPRSQYMVIDSGSDIVWVQCQPCTRCYQQSDPV 190
           V +FG+D++SGM++GSGEYFVRIGVGSPPR QYMVIDSGSD+VWVQCQPC  CY+QSDPV
Sbjct: 128 VNDFGSDIVSGMDQGSGEYFVRIGVGSPPRDQYMVIDSGSDMVWVQCQPCKLCYKQSDPV 187

Query: 191 FDPADSSSFAGVSCGSAVCDRLENTGCNAGRCRYEVSYGDGSYTKGTLALETLTLGQVMI 250
           FDPA S S+ GVSCGS+VCDR+EN+GC++G CRYEV YGDGSYTKGTLALETLT  + ++
Sbjct: 188 FDPAKSGSYTGVSCGSSVCDRIENSGCHSGGCRYEVMYGDGSYTKGTLALETLTFAKTVV 247

Query: 251 RDVAIGCGHTNQGMFIGAAGLLGLGGGSMSFIGQLGGQTGGAFSYCLVSRGTGSTGTLEF 310
           R+VA+GCGH N+GMFIGAAGLLG+GGGSMSF+GQL GQTGGAF YCLVSRGT STG+L F
Sbjct: 248 RNVAMGCGHRNRGMFIGAAGLLGIGGGSMSFVGQLSGQTGGAFGYCLVSRGTDSTGSLVF 307

Query: 311 GRGTLPVGATWISLIRNPRAPSFYYIGLAGIGVGGVRVPVPEETFQLTEYGTNGVVMDTG 370
           GR  LPVGA+W+ L+RNPRAPSFYY+GL G+GVGGVR+P+P+  F LTE G  GVVMDTG
Sbjct: 308 GREALPVGASWVPLVRNPRAPSFYYVGLKGLGVGGVRIPLPDGVFDLTETGDGGVVMDTG 367

Query: 371 TAVTRLPTAAYVALRDSFTAQTSNLPRAPGVSIFDTCYDLNGFESVRVPTVSFYFSDGPV 430
           TAVTRLPTAAYVA RD F +QT+NLPRA GVSIFDTCYDL+GF SVRVPTVSFYF++GPV
Sbjct: 368 TAVTRLPTAAYVAFRDGFKSQTANLPRASGVSIFDTCYDLSGFVSVRVPTVSFYFTEGPV 427

Query: 431 LTLPAKNFLIPVDGGGTFCLAFAPSPSGLSIIGNIQQEGIQISFDGANGFVGFGPNIC 482
           LTLPA+NFL+PVD  GT+C AFA SP+GLSIIGNIQQEGIQ+SFDGANGFVGFGPN+C
Sbjct: 428 LTLPARNFLMPVDDSGTYCFAFAASPTGLSIIGNIQQEGIQVSFDGANGFVGFGPNVC 470

BLAST of Cla97C02G040000 vs. ExPASy Swiss-Prot
Match: Q9LNJ3 (Aspartyl protease family protein 2 OS=Arabidopsis thaliana OX=3702 GN=APF2 PE=2 SV=1)

HSP 1 Score: 391.0 bits (1003), Expect = 2.0e-107
Identity = 218/484 (45.04%), Postives = 297/484 (61.36%), Query Frame = 0

Query: 8   SLLFFLHLLFFS-AAVAARPSPPTKF--QYLNVKATKLDF---NDGQILHTLNFSDGHRQ 67
           +LLF L   F S  + ++ PS  T F   +    A+ + F   +D + L    F  G   
Sbjct: 7   ALLFSLCFFFLSLPSFSSLPSFQTLFPNSHSLPCASPVSFQPDSDSESLLESEFESGSDS 66

Query: 68  ASGHKSDNNTFKLNLVHRDKLSHVHGHRHGFNERIKRDAIRVATLVRRLSHGGGAVQDNK 127
            S     +++  LNL H D LS        F+ R++RD+ RV ++    +   G    + 
Sbjct: 67  ES-----SSSITLNLDHIDALSSNKTPDELFSSRLQRDSRRVKSIATLAAQIPGRNVTHA 126

Query: 128 YKVANFGTDVISGMEEGSGEYFVRIGVGSPPRSQYMVIDSGSDIVWVQCQPCTRCYQQSD 187
            +   F + V+SG+ +GSGEYF R+GVG+P R  YMV+D+GSDIVW+QC PC RCY QSD
Sbjct: 127 PRPGGFSSSVVSGLSQGSGEYFTRLGVGTPARYVYMVLDTGSDIVWLQCAPCRRCYSQSD 186

Query: 188 PVFDPADSSSFAGVSCGSAVCDRLENTGCNAGR--CRYEVSYGDGSYTKGTLALETLTLG 247
           P+FDP  S ++A + C S  C RL++ GCN  R  C Y+VSYGDGS+T G  + ETLT  
Sbjct: 187 PIFDPRKSKTYATIPCSSPHCRRLDSAGCNTRRKTCLYQVSYGDGSFTVGDFSTETLTFR 246

Query: 248 QVMIRDVAIGCGHTNQGMFIGAAGLLGLGGGSMSFIGQLGGQTGGAFSYCLVSRGTGS-T 307
           +  ++ VA+GCGH N+G+F+GAAGLLGLG G +SF GQ G +    FSYCLV R   S  
Sbjct: 247 RNRVKGVALGCGHDNEGLFVGAAGLLGLGKGKLSFPGQTGHRFNQKFSYCLVDRSASSKP 306

Query: 308 GTLEFGRGTLPVGATWISLIRNPRAPSFYYIGLAGIGVGGVRVP-VPEETFQLTEYGTNG 367
            ++ FG   +   A +  L+ NP+  +FYY+GL GI VGG RVP V    F+L + G  G
Sbjct: 307 SSVVFGNAAVSRIARFTPLLSNPKLDTFYYVGLLGISVGGTRVPGVTASLFKLDQIGNGG 366

Query: 368 VVMDTGTAVTRLPTAAYVALRDSFTAQTSNLPRAPGVSIFDTCYDLNGFESVRVPTVSFY 427
           V++D+GT+VTRL   AY+A+RD+F      L RAP  S+FDTC+DL+    V+VPTV  +
Sbjct: 367 VIIDSGTSVTRLIRPAYIAMRDAFRVGAKTLKRAPDFSLFDTCFDLSNMNEVKVPTVVLH 426

Query: 428 FSDGPVLTLPAKNFLIPVDGGGTFCLAFAPSPSGLSIIGNIQQEGIQISFDGANGFVGFG 482
           F  G  ++LPA N+LIPVD  G FC AFA +  GLSIIGNIQQ+G ++ +D A+  VGF 
Sbjct: 427 FR-GADVSLPATNYLIPVDTNGKFCFAFAGTMGGLSIIGNIQQQGFRVVYDLASSRVGFA 484

BLAST of Cla97C02G040000 vs. ExPASy Swiss-Prot
Match: Q9LS40 (Protein ASPARTIC PROTEASE IN GUARD CELL 1 OS=Arabidopsis thaliana OX=3702 GN=ASPG1 PE=1 SV=1)

HSP 1 Score: 390.6 bits (1002), Expect = 2.6e-107
Identity = 190/399 (47.62%), Postives = 267/399 (66.92%), Query Frame = 0

Query: 95  RIKRDAIRVATLVRRLSHGGGAV----------QDNKYKVANFGTDVISGMEEGSGEYFV 154
           R++RD+ RVA +V ++      V          +D +Y+  +  T V+SG  +GSGEYF 
Sbjct: 105 RLERDSSRVAGIVAKIRFAVEGVDRSDLKPVYNEDTRYQTEDLTTPVVSGASQGSGEYFS 164

Query: 155 RIGVGSPPRSQYMVIDSGSDIVWVQCQPCTRCYQQSDPVFDPADSSSFAGVSCGSAVCDR 214
           RIGVG+P +  Y+V+D+GSD+ W+QC+PC  CYQQSDPVF+P  SS++  ++C +  C  
Sbjct: 165 RIGVGTPAKEMYLVLDTGSDVNWIQCEPCADCYQQSDPVFNPTSSSTYKSLTCSAPQCSL 224

Query: 215 LENTGCNAGRCRYEVSYGDGSYTKGTLALETLTLGQV-MIRDVAIGCGHTNQGMFIGAAG 274
           LE + C + +C Y+VSYGDGS+T G LA +T+T G    I +VA+GCGH N+G+F GAAG
Sbjct: 225 LETSACRSNKCLYQVSYGDGSFTVGELATDTVTFGNSGKINNVALGCGHDNEGLFTGAAG 284

Query: 275 LLGLGGGSMSFIGQLGGQTGGAFSYCLVSRGTGSTGTLEFGRGTLPVGATWISLIRNPRA 334
           LLGLGGG +S   Q+      +FSYCLV R +G + +L+F    L  G     L+RN + 
Sbjct: 285 LLGLGGGVLSITNQM---KATSFSYCLVDRDSGKSSSLDFNSVQLGGGDATAPLLRNKKI 344

Query: 335 PSFYYIGLAGIGVGGVRVPVPEETFQLTEYGTNGVVMDTGTAVTRLPTAAYVALRDSFTA 394
            +FYY+GL+G  VGG +V +P+  F +   G+ GV++D GTAVTRL T AY +LRD+F  
Sbjct: 345 DTFYYVGLSGFSVGGEKVVLPDAIFDVDASGSGGVILDCGTAVTRLQTQAYNSLRDAFLK 404

Query: 395 QTSNLPR-APGVSIFDTCYDLNGFESVRVPTVSFYFSDGPVLTLPAKNFLIPVDGGGTFC 454
            T NL + +  +S+FDTCYD +   +V+VPTV+F+F+ G  L LPAKN+LIPVD  GTFC
Sbjct: 405 LTVNLKKGSSSISLFDTCYDFSSLSTVKVPTVAFHFTGGKSLDLPAKNYLIPVDDSGTFC 464

Query: 455 LAFAPSPSGLSIIGNIQQEGIQISFDGANGFVGFGPNIC 482
            AFAP+ S LSIIGN+QQ+G +I++D +   +G   N C
Sbjct: 465 FAFAPTSSSLSIIGNVQQQGTRITYDLSKNVIGLSGNKC 500

BLAST of Cla97C02G040000 vs. ExPASy Swiss-Prot
Match: Q8S9J6 (Aspartyl protease family protein At5g10770 OS=Arabidopsis thaliana OX=3702 GN=At5g10770 PE=2 SV=1)

HSP 1 Score: 273.1 bits (697), Expect = 6.1e-72
Identity = 164/415 (39.52%), Postives = 219/415 (52.77%), Query Frame = 0

Query: 88  HRHGFNERIKRDAIRVATLVRRLSHGGGAVQDNKYKVA-NFGTDVIS-----------GM 147
           HRHG   R+          V  L      V     K++    TD +S           G 
Sbjct: 66  HRHGTCSRLNNGKATSPDHVEILRLDQARVNSIHSKLSKKLATDHVSESKSTDLPAKDGS 125

Query: 148 EEGSGEYFVRIGVGSPPRSQYMVIDSGSDIVWVQCQPCTR-CYQQSDPVFDPADSSSFAG 207
             GSG Y V +G+G+P     ++ D+GSD+ W QCQPC R CY Q +P+F+P+ S+S+  
Sbjct: 126 TLGSGNYIVTVGLGTPKNDLSLIFDTGSDLTWTQCQPCVRTCYDQKEPIFNPSKSTSYYN 185

Query: 208 VSCGSAVCDRLENT-----GCNAGRCRYEVSYGDGSYTKGTLALETLTLGQVMIRD-VAI 267
           VSC SA C  L +       C+A  C Y + YGD S++ G LA E  TL    + D V  
Sbjct: 186 VSCSSAACGSLSSATGNAGSCSASNCIYGIQYGDQSFSVGFLAKEKFTLTNSDVFDGVYF 245

Query: 268 GCGHTNQGMFIGAAGLLGLGGGSMSFIGQLGGQTGGAFSYCLVSRGTGSTGTLEFGRGTL 327
           GCG  NQG+F G AGLLGLG   +SF  Q        FSYCL S     TG L FG   +
Sbjct: 246 GCGENNQGLFTGVAGLLGLGRDKLSFPSQTATAYNKIFSYCLPS-SASYTGHLTFGSAGI 305

Query: 328 PVGATWISLIRNPRAPSFYYIGLAGIGVGGVRVPVPEETFQLTEYGTNGVVMDTGTAVTR 387
                +  +       SFY + +  I VGG ++P+P      T + T G ++D+GT +TR
Sbjct: 306 SRSVKFTPISTITDGTSFYGLNIVAITVGGQKLPIPS-----TVFSTPGALIDSGTVITR 365

Query: 388 LPTAAYVALRDSFTAQTSNLPRAPGVSIFDTCYDLNGFESVRVPTVSFYFSDGPVLTLPA 447
           LP  AY ALR SF A+ S  P   GVSI DTC+DL+GF++V +P V+F FS G V+ L +
Sbjct: 366 LPPKAYAALRSSFKAKMSKYPTTSGVSILDTCFDLSGFKTVTIPKVAFSFSGGAVVELGS 425

Query: 448 KNFLIPVDGGGTFCLAFA--PSPSGLSIIGNIQQEGIQISFDGANGFVGFGPNIC 482
           K  +  V      CLAFA     S  +I GN+QQ+ +++ +DGA G VGF PN C
Sbjct: 426 KG-IFYVFKISQVCLAFAGNSDDSNAAIFGNVQQQTLEVVYDGAGGRVGFAPNGC 473

BLAST of Cla97C02G040000 vs. ExPASy Swiss-Prot
Match: Q9LEW3 (Aspartyl protease AED1 OS=Arabidopsis thaliana OX=3702 GN=AED1 PE=2 SV=1)

HSP 1 Score: 261.9 bits (668), Expect = 1.4e-68
Identity = 157/420 (37.38%), Postives = 220/420 (52.38%), Query Frame = 0

Query: 66  KSDNNTFKLNLVH-RDKLSHVHGH-RHGFNERIKRDAIRVATLVRRLSHGGGAVQDNKYK 125
           K+ N    L +VH     SH+    R   +E I+RD  RV ++  +LS       +   +
Sbjct: 57  KASNTKSSLRVVHMHGACSHLSSDARVDHDEIIRRDQARVESIYSKLSKNSA---NEVSE 116

Query: 126 VANFGTDVISGMEEGSGEYFVRIGVGSPPRSQYMVIDSGSDIVWVQCQPCT-RCYQQSDP 185
             +      SG+  GSG Y V IG+G+P     +V D+GSD+ W QC+PC   CY Q +P
Sbjct: 117 AKSTELPAKSGITLGSGNYIVTIGIGTPKHDLSLVFDTGSDLTWTQCEPCLGSCYSQKEP 176

Query: 186 VFDPADSSSFAGVSCGSAVCDRLENTGCNAGRCRYEVSYGDGSYTKGTLALETLTL-GQV 245
            F+P+ SS++  VSC S +C+  E+  C+A  C Y + YGD S+T+G LA E  TL    
Sbjct: 177 KFNPSSSSTYQNVSCSSPMCEDAES--CSASNCVYSIVYGDKSFTQGFLAKEKFTLTNSD 236

Query: 246 MIRDVAIGCGHTNQGMFIGAAGLLGLGGGSMSFIGQLGGQTGGAFSYCLVSRGTGSTGTL 305
           ++ DV  GCG  NQG+F G AGLLGLG G +S   Q        FSYCL S  + STG L
Sbjct: 237 VLEDVYFGCGENNQGLFDGVAGLLGLGPGKLSLPAQTTTTYNNIFSYCLPSFTSNSTGHL 296

Query: 306 EFGRGTLPVGATWISLIRNPRAPSFYYIGLAGIGVGGVRVPVPEETFQLTEYGTNGVVMD 365
            FG   +     +  +   P A + Y I + GI VG   + +   +F      T G ++D
Sbjct: 297 TFGSAGISESVKFTPISSFPSAFN-YGIDIIGISVGDKELAITPNSF-----STEGAIID 356

Query: 366 TGTAVTRLPTAAYVALRDSFTAQTSNLPRAPGVSIFDTCYDLNGFESVRVPTVSFYFSDG 425
           +GT  TRLPT  Y  LR  F  + S+     G  +FDTCYD  G ++V  PT++F F+  
Sbjct: 357 SGTVFTRLPTKVYAELRSVFKEKMSSYKSTSGYGLFDTCYDFTGLDTVTYPTIAFSFAGS 416

Query: 426 PVLTLPAKNFLIPVDGGGTFCLAFAPSPSGLSIIGNIQQEGIQISFDGANGFVGFGPNIC 482
            V+ L      +P+      CLAFA +    +I GN+QQ  + + +D A G VGF PN C
Sbjct: 417 TVVELDGSGISLPIK-ISQVCLAFAGNDDLPAIFGNVQQTTLDVVYDVAGGRVGFAPNGC 464

BLAST of Cla97C02G040000 vs. ExPASy TrEMBL
Match: A0A1S3BEG7 (protein ASPARTIC PROTEASE IN GUARD CELL 2-like OS=Cucumis melo OX=3656 GN=LOC103488783 PE=4 SV=1)

HSP 1 Score: 899.4 bits (2323), Expect = 6.4e-258
Identity = 445/480 (92.71%), Postives = 460/480 (95.83%), Query Frame = 0

Query: 2   FLQQPSSLLFFLHLLFFSAAVAARPSPPTKFQYLNVKATKLDFNDGQILHTLNFSDGHRQ 61
           F Q  SSLLFFL +L   AA AARPS PTKFQYLNVKATKLDFNDGQILHTLNFSD HRQ
Sbjct: 3   FKQLISSLLFFLAVLVVVAA-AARPSSPTKFQYLNVKATKLDFNDGQILHTLNFSDAHRQ 62

Query: 62  ASGHKSDNNTFKLNLVHRDKLSHVHGHRHGFNERIKRDAIRVATLVRRLSHGGGAVQDNK 121
            SGHKS N+TFKLNL+HRDKLSHVHGHR GFN+R+KRDAIRVATLVRRLSHG  AV+D++
Sbjct: 63  VSGHKSHNDTFKLNLLHRDKLSHVHGHRRGFNDRMKRDAIRVATLVRRLSHGAAAVEDSR 122

Query: 122 YKVANFGTDVISGMEEGSGEYFVRIGVGSPPRSQYMVIDSGSDIVWVQCQPCTRCYQQSD 181
           +KVANF TDVISGMEEGSGEYFVRIGVGSPPRSQYMVIDSGSDIVWVQC+PC+RCYQQSD
Sbjct: 123 FKVANFATDVISGMEEGSGEYFVRIGVGSPPRSQYMVIDSGSDIVWVQCKPCSRCYQQSD 182

Query: 182 PVFDPADSSSFAGVSCGSAVCDRLENTGCNAGRCRYEVSYGDGSYTKGTLALETLTLGQV 241
           PVFDPADSSSFAGVSCGS VCDRLENTGCNAGRCRYEVSYGDGSYTKGTLALETLT+GQV
Sbjct: 183 PVFDPADSSSFAGVSCGSDVCDRLENTGCNAGRCRYEVSYGDGSYTKGTLALETLTVGQV 242

Query: 242 MIRDVAIGCGHTNQGMFIGAAGLLGLGGGSMSFIGQLGGQTGGAFSYCLVSRGTGSTGTL 301
           MIRDVAIGCGHTNQGMFIGAAGLLGLGGGSMSFIGQLGGQTGGAFSYCLVSRGTGSTGTL
Sbjct: 243 MIRDVAIGCGHTNQGMFIGAAGLLGLGGGSMSFIGQLGGQTGGAFSYCLVSRGTGSTGTL 302

Query: 302 EFGRGTLPVGATWISLIRNPRAPSFYYIGLAGIGVGGVRVPVPEETFQLTEYGTNGVVMD 361
           EFGRG LPVGATWISLIRNPRAPSFYYIGLAGIGVGGVRV +PEETFQLTE+GTNGVVMD
Sbjct: 303 EFGRGALPVGATWISLIRNPRAPSFYYIGLAGIGVGGVRVSIPEETFQLTEFGTNGVVMD 362

Query: 362 TGTAVTRLPTAAYVALRDSFTAQTSNLPRAPGVSIFDTCYDLNGFESVRVPTVSFYFSDG 421
           TGTAVTRLPT+AYVALRDSFTAQTSNLPRAPGVSIFDTCYDLNGFESVRVPTVSFYFSDG
Sbjct: 363 TGTAVTRLPTSAYVALRDSFTAQTSNLPRAPGVSIFDTCYDLNGFESVRVPTVSFYFSDG 422

Query: 422 PVLTLPAKNFLIPVDGGGTFCLAFAPSPSGLSIIGNIQQEGIQISFDGANGFVGFGPNIC 481
           P LTLPAKNFLIPVDGGGTFCLAFAPSPSGLSIIGNIQQEGIQISFDGANGFVGFGPNIC
Sbjct: 423 PALTLPAKNFLIPVDGGGTFCLAFAPSPSGLSIIGNIQQEGIQISFDGANGFVGFGPNIC 481

BLAST of Cla97C02G040000 vs. ExPASy TrEMBL
Match: A0A0A0KPR4 (Peptidase A1 domain-containing protein OS=Cucumis sativus OX=3659 GN=Csa_5G579060 PE=4 SV=1)

HSP 1 Score: 887.5 bits (2292), Expect = 2.5e-254
Identity = 440/482 (91.29%), Postives = 455/482 (94.40%), Query Frame = 0

Query: 1   MFLQQPSSLLFFLHLLFFSAAVAARPSPPTKFQYLNVKATKLDFNDGQILHTLNFSDGHR 60
           MF +Q  S L FL ++   AA AAR S  TKFQYLNVKATKLDFNDGQILH LNFSDGHR
Sbjct: 1   MFFKQLFSSLLFLLVVIVGAAAAARLSSSTKFQYLNVKATKLDFNDGQILHALNFSDGHR 60

Query: 61  QASGHKSDNNTFKLNLVHRDKLSHVHGHRHGFNERIKRDAIRVATLVRRLSHGG-GAVQD 120
           Q SG+KSDNNTFKLNL+HRDKLSHVHGHR GFN+R+KRDAIRVATLVRRLSHG   AV+D
Sbjct: 61  QVSGYKSDNNTFKLNLLHRDKLSHVHGHRRGFNDRMKRDAIRVATLVRRLSHGAPAAVKD 120

Query: 121 NKYKVANFGTDVISGMEEGSGEYFVRIGVGSPPRSQYMVIDSGSDIVWVQCQPCTRCYQQ 180
           ++YKVANF TDVISGME GSGEYFVRIGVGSPPR+QYMVIDSGSDIVWVQC+PC+RCYQQ
Sbjct: 121 SRYKVANFATDVISGMEAGSGEYFVRIGVGSPPRNQYMVIDSGSDIVWVQCKPCSRCYQQ 180

Query: 181 SDPVFDPADSSSFAGVSCGSAVCDRLENTGCNAGRCRYEVSYGDGSYTKGTLALETLTLG 240
           SDPVFDPADSSSFAGVSCGS VCDRLENTGCNAGRCRYEVSYGDGSYTKGTLALETLT+G
Sbjct: 181 SDPVFDPADSSSFAGVSCGSDVCDRLENTGCNAGRCRYEVSYGDGSYTKGTLALETLTVG 240

Query: 241 QVMIRDVAIGCGHTNQGMFIGAAGLLGLGGGSMSFIGQLGGQTGGAFSYCLVSRGTGSTG 300
           QVMIRDVAIGCGHTNQGMFIGAAGLLGLGGGSMSFIGQLGGQTGGAFSYCLVSRGTGSTG
Sbjct: 241 QVMIRDVAIGCGHTNQGMFIGAAGLLGLGGGSMSFIGQLGGQTGGAFSYCLVSRGTGSTG 300

Query: 301 TLEFGRGTLPVGATWISLIRNPRAPSFYYIGLAGIGVGGVRVPVPEETFQLTEYGTNGVV 360
            LEFGRG LPVGATWISLIRNPRAPSFYYIGLAGIGVGGVRV VPEETFQLTEYGTNGVV
Sbjct: 301 ALEFGRGALPVGATWISLIRNPRAPSFYYIGLAGIGVGGVRVSVPEETFQLTEYGTNGVV 360

Query: 361 MDTGTAVTRLPTAAYVALRDSFTAQTSNLPRAPGVSIFDTCYDLNGFESVRVPTVSFYFS 420
           MDTGTAVTR PTAAYVA RDSFTAQTSNLPRAPGVSIFDTCYDLNGFESVRVPTVSFYFS
Sbjct: 361 MDTGTAVTRFPTAAYVAFRDSFTAQTSNLPRAPGVSIFDTCYDLNGFESVRVPTVSFYFS 420

Query: 421 DGPVLTLPAKNFLIPVDGGGTFCLAFAPSPSGLSIIGNIQQEGIQISFDGANGFVGFGPN 480
           DGPVLTLPA+NFLIPVDGGGTFCLAFAPSPSGLSIIGNIQQEGIQISFDGANGFVGFGPN
Sbjct: 421 DGPVLTLPARNFLIPVDGGGTFCLAFAPSPSGLSIIGNIQQEGIQISFDGANGFVGFGPN 480

Query: 481 IC 482
           IC
Sbjct: 481 IC 482

BLAST of Cla97C02G040000 vs. ExPASy TrEMBL
Match: A0A5A7SVY2 (Protein ASPARTIC PROTEASE IN GUARD CELL 2-like OS=Cucumis melo var. makuwa OX=1194695 GN=E6C27_scaffold65G00740 PE=4 SV=1)

HSP 1 Score: 864.8 bits (2233), Expect = 1.7e-247
Identity = 432/480 (90.00%), Postives = 447/480 (93.12%), Query Frame = 0

Query: 2   FLQQPSSLLFFLHLLFFSAAVAARPSPPTKFQYLNVKATKLDFNDGQILHTLNFSDGHRQ 61
           F Q  SSLLFFL +L   AA AARPS PTKFQYLNVKATKLDFNDGQILHTLNFSD HRQ
Sbjct: 3   FKQLISSLLFFLAVLVVVAA-AARPSSPTKFQYLNVKATKLDFNDGQILHTLNFSDAHRQ 62

Query: 62  ASGHKSDNNTFKLNLVHRDKLSHVHGHRHGFNERIKRDAIRVATLVRRLSHGGGAVQDNK 121
            SGHKS N+TFKLNL+HRDKLSHVHGHR GFN+R+KRDAIRVATLVRRLSHG  AV+D++
Sbjct: 63  VSGHKSHNDTFKLNLLHRDKLSHVHGHRRGFNDRMKRDAIRVATLVRRLSHGAAAVEDSR 122

Query: 122 YKVANFGTDVISGMEEGSGEYFVRIGVGSPPRSQYMVIDSGSDIVWVQCQPCTRCYQQSD 181
           +K              GSGEYFVRIGVGSPPRSQYMVIDSGSDIVWVQC+PC+RCYQQSD
Sbjct: 123 FK--------------GSGEYFVRIGVGSPPRSQYMVIDSGSDIVWVQCKPCSRCYQQSD 182

Query: 182 PVFDPADSSSFAGVSCGSAVCDRLENTGCNAGRCRYEVSYGDGSYTKGTLALETLTLGQV 241
           PVFDPADSSSFAGVSCGS VCDRLENTGCNAGRCRYEVSYGDGSYTKGTLALETLT+GQV
Sbjct: 183 PVFDPADSSSFAGVSCGSDVCDRLENTGCNAGRCRYEVSYGDGSYTKGTLALETLTVGQV 242

Query: 242 MIRDVAIGCGHTNQGMFIGAAGLLGLGGGSMSFIGQLGGQTGGAFSYCLVSRGTGSTGTL 301
           MIRDVAIGCGHTNQGMFIGAAGLLGLGGGSMSFIGQLGGQTGGAFSYCLVSRGTGSTGTL
Sbjct: 243 MIRDVAIGCGHTNQGMFIGAAGLLGLGGGSMSFIGQLGGQTGGAFSYCLVSRGTGSTGTL 302

Query: 302 EFGRGTLPVGATWISLIRNPRAPSFYYIGLAGIGVGGVRVPVPEETFQLTEYGTNGVVMD 361
           EFGRG LPVGATWISLIRNPRAPSFYYIGLAGIGVGGVRV +PEETFQLTE+GTNGVVMD
Sbjct: 303 EFGRGALPVGATWISLIRNPRAPSFYYIGLAGIGVGGVRVSIPEETFQLTEFGTNGVVMD 362

Query: 362 TGTAVTRLPTAAYVALRDSFTAQTSNLPRAPGVSIFDTCYDLNGFESVRVPTVSFYFSDG 421
           TGTAVTRLPT+AYVALRDSFTAQTSNLPRAPGVSIFDTCYDLNGFESVRVPTVSFYFSDG
Sbjct: 363 TGTAVTRLPTSAYVALRDSFTAQTSNLPRAPGVSIFDTCYDLNGFESVRVPTVSFYFSDG 422

Query: 422 PVLTLPAKNFLIPVDGGGTFCLAFAPSPSGLSIIGNIQQEGIQISFDGANGFVGFGPNIC 481
           P LTLPAKNFLIPVDGGGTFCLAFAPSPSGLSIIGNIQQEGIQISFDGANGFVGFGPNIC
Sbjct: 423 PALTLPAKNFLIPVDGGGTFCLAFAPSPSGLSIIGNIQQEGIQISFDGANGFVGFGPNIC 467

BLAST of Cla97C02G040000 vs. ExPASy TrEMBL
Match: A0A6J1KA06 (protein ASPARTIC PROTEASE IN GUARD CELL 2-like OS=Cucurbita maxima OX=3661 GN=LOC111493608 PE=4 SV=1)

HSP 1 Score: 834.7 bits (2155), Expect = 1.9e-238
Identity = 416/486 (85.60%), Postives = 440/486 (90.53%), Query Frame = 0

Query: 1   MFLQQPSS-LLFFLHLLFFSAAVA--ARPSPPTKFQYLNVKATKLDFNDGQILHTLNFSD 60
           MFL+ PSS LL  LHLLF +AA A  ARPS P  F YLNVK TKL+ N  QIL TLNFSD
Sbjct: 1   MFLKHPSSPLLLLLHLLFLAAAAAALARPSLPPNFLYLNVKPTKLELNHTQILPTLNFSD 60

Query: 61  GHRQASGHKSDNNTFKLNLVHRDKLSHVHG--HRHGFNERIKRDAIRVATLVRRLSHGGG 120
            H Q +  K  N T KLNL+HRDKL HVH   HRH FNERIKRDAIRVATLVRRLSH   
Sbjct: 61  AHLQVTAQKFHNTTLKLNLLHRDKLPHVHAHRHRHAFNERIKRDAIRVATLVRRLSHTAA 120

Query: 121 AVQDNKYKVANFGTDVISGMEEGSGEYFVRIGVGSPPRSQYMVIDSGSDIVWVQCQPCTR 180
           AVQDN YKVANF T V+SGMEEGSGE+FVR+GVGSPPRSQYMVIDSGSDIVWVQCQPCT+
Sbjct: 121 AVQDNSYKVANFATGVVSGMEEGSGEFFVRVGVGSPPRSQYMVIDSGSDIVWVQCQPCTQ 180

Query: 181 CYQQSDPVFDPADSSSFAGVSCGSAVCDRLENTGCNAGRCRYEVSYGDGSYTKGTLALET 240
           CYQQSDPVF+PADSSSFAGVSCGSAVC RL+NT C+AGRCRYEVSYGDGSYTKGTLALET
Sbjct: 181 CYQQSDPVFNPADSSSFAGVSCGSAVCGRLDNTDCHAGRCRYEVSYGDGSYTKGTLALET 240

Query: 241 LTLGQVMIRDVAIGCGHTNQGMFIGAAGLLGLGGGSMSFIGQLGGQTGGAFSYCLVSRGT 300
           +T+GQVMIRDVAIGCGHTNQGMFIGAAGLLGLGGGSMSFIGQLGGQTGGAFSYCLVSR T
Sbjct: 241 ITMGQVMIRDVAIGCGHTNQGMFIGAAGLLGLGGGSMSFIGQLGGQTGGAFSYCLVSRDT 300

Query: 301 GSTGTLEFGRGTLPVGATWISLIRNPRAPSFYYIGLAGIGVGGVRVPVPEETFQLTEYGT 360
           GSTGTLEFGRG +PVGATWISLIRNPRAPSFYYIGLAG+GVGGVRVP+PEETFQL+EYGT
Sbjct: 301 GSTGTLEFGRGAMPVGATWISLIRNPRAPSFYYIGLAGVGVGGVRVPIPEETFQLSEYGT 360

Query: 361 NGVVMDTGTAVTRLPTAAYVALRDSFTAQTSNLPRAPGVSIFDTCYDLNGFESVRVPTVS 420
           NGVVMDTGTAVTRLPTAAY A RD+FTAQT+NLPRA GVSIFDTC+DLNGFES+RVPTVS
Sbjct: 361 NGVVMDTGTAVTRLPTAAYEAFRDAFTAQTNNLPRAGGVSIFDTCFDLNGFESIRVPTVS 420

Query: 421 FYFSDGPVLTLPAKNFLIPVDGGGTFCLAFAPSPSGLSIIGNIQQEGIQISFDGANGFVG 480
           FYFSDGPVLTLPAKNFLIPV+G GTFCLAFAPSPSGLSIIGNIQQEGIQIS DGANGFVG
Sbjct: 421 FYFSDGPVLTLPAKNFLIPVNGAGTFCLAFAPSPSGLSIIGNIQQEGIQISIDGANGFVG 480

Query: 481 FGPNIC 482
           FGPN+C
Sbjct: 481 FGPNVC 486

BLAST of Cla97C02G040000 vs. ExPASy TrEMBL
Match: A0A6J1GXH5 (protein ASPARTIC PROTEASE IN GUARD CELL 2-like OS=Cucurbita moschata OX=3662 GN=LOC111458389 PE=4 SV=1)

HSP 1 Score: 826.6 bits (2134), Expect = 5.2e-236
Identity = 411/489 (84.05%), Postives = 436/489 (89.16%), Query Frame = 0

Query: 1   MFLQQPSS-LLFFLHLLFFSAAVA---ARPSPPTKFQYLNVKATKLDFNDGQILHTLNFS 60
           MFL+ PSS LL  LHLL F AA +   ARPSPP  F YLNVK TKL+ N   IL TLNFS
Sbjct: 1   MFLKHPSSPLLLLLHLLLFLAAASAALARPSPPPNFLYLNVKPTKLELNHTHILPTLNFS 60

Query: 61  DGHRQASGHKSDNNTFKLNLVHRDKLSHVHG----HRHGFNERIKRDAIRVATLVRRLSH 120
             H Q +  K  N T KLNL+HRDKL HVH     HRH FNERIKRDAIRVATLVRRLSH
Sbjct: 61  HAHLQVTAQKFHNTTLKLNLLHRDKLPHVHALRHRHRHAFNERIKRDAIRVATLVRRLSH 120

Query: 121 GGGAVQDNKYKVANFGTDVISGMEEGSGEYFVRIGVGSPPRSQYMVIDSGSDIVWVQCQP 180
              AVQDN YKVA+F TDV+SGMEEGSGE+FVR+GVGSPPRSQYMVIDSGSDIVWVQCQP
Sbjct: 121 AAAAVQDNSYKVASFATDVVSGMEEGSGEFFVRVGVGSPPRSQYMVIDSGSDIVWVQCQP 180

Query: 181 CTRCYQQSDPVFDPADSSSFAGVSCGSAVCDRLENTGCNAGRCRYEVSYGDGSYTKGTLA 240
           CT+CYQQSDPVF+PADSSSF GVSCGS VCDRL+NT C+AGRCRYEVSYGDGSYTKGTLA
Sbjct: 181 CTQCYQQSDPVFNPADSSSFEGVSCGSTVCDRLDNTDCHAGRCRYEVSYGDGSYTKGTLA 240

Query: 241 LETLTLGQVMIRDVAIGCGHTNQGMFIGAAGLLGLGGGSMSFIGQLGGQTGGAFSYCLVS 300
           LET+T+GQVMIRDVAIGCGHTNQGMFIGAAGLLGLGGGSMS IGQLGGQTGGAFSYCLVS
Sbjct: 241 LETITMGQVMIRDVAIGCGHTNQGMFIGAAGLLGLGGGSMSLIGQLGGQTGGAFSYCLVS 300

Query: 301 RGTGSTGTLEFGRGTLPVGATWISLIRNPRAPSFYYIGLAGIGVGGVRVPVPEETFQLTE 360
           R TGSTGTLEFGRG +PVGATWISLIRNPRAPSFYYIGLAG+GVGGVRVP+PEETFQL+E
Sbjct: 301 RDTGSTGTLEFGRGAMPVGATWISLIRNPRAPSFYYIGLAGVGVGGVRVPIPEETFQLSE 360

Query: 361 YGTNGVVMDTGTAVTRLPTAAYVALRDSFTAQTSNLPRAPGVSIFDTCYDLNGFESVRVP 420
           YGTNGVVMDTGTAVTRLPTAAY A RD+F AQT+NLPRA GVSIFDTC+DLNGFES+RVP
Sbjct: 361 YGTNGVVMDTGTAVTRLPTAAYEAFRDAFIAQTNNLPRAGGVSIFDTCFDLNGFESIRVP 420

Query: 421 TVSFYFSDGPVLTLPAKNFLIPVDGGGTFCLAFAPSPSGLSIIGNIQQEGIQISFDGANG 480
           TVSFYFSDGPVLTLPAKNFLIPV+G GTFCLAFAPSPSGLSIIGNIQQEGIQIS DGANG
Sbjct: 421 TVSFYFSDGPVLTLPAKNFLIPVNGAGTFCLAFAPSPSGLSIIGNIQQEGIQISIDGANG 480

Query: 481 FVGFGPNIC 482
           FVGFGPN+C
Sbjct: 481 FVGFGPNVC 489

BLAST of Cla97C02G040000 vs. TAIR 10
Match: AT3G20015.1 (Eukaryotic aspartyl protease family protein )

HSP 1 Score: 638.3 bits (1645), Expect = 5.1e-183
Identity = 314/478 (65.69%), Postives = 378/478 (79.08%), Query Frame = 0

Query: 11  FFLHLLFFSAAVAARPSPPTKF-----QYLNVKATKLDFNDGQILHTLNFSDGHRQASGH 70
           FFLHL    ++ ++   P  +        L V AT  DFN+       +FSD        
Sbjct: 8   FFLHLHLHLSSSSSISFPDFQIIDVLQPPLTVTATLPDFNN------THFSD-------- 67

Query: 71  KSDNNTFKLNLVHRDKLSHV--HGHRHGFNERIKRDAIRVATLVRRLSHGGGAVQDNKYK 130
              ++ + L L+HRD+   V    H H  + R++RD  RV+ ++RR+S       D++Y+
Sbjct: 68  -ESSSKYTLRLLHRDRFPSVTYRNHHHRLHARMRRDTDRVSAILRRISGKVIPSSDSRYE 127

Query: 131 VANFGTDVISGMEEGSGEYFVRIGVGSPPRSQYMVIDSGSDIVWVQCQPCTRCYQQSDPV 190
           V +FG+D++SGM++GSGEYFVRIGVGSPPR QYMVIDSGSD+VWVQCQPC  CY+QSDPV
Sbjct: 128 VNDFGSDIVSGMDQGSGEYFVRIGVGSPPRDQYMVIDSGSDMVWVQCQPCKLCYKQSDPV 187

Query: 191 FDPADSSSFAGVSCGSAVCDRLENTGCNAGRCRYEVSYGDGSYTKGTLALETLTLGQVMI 250
           FDPA S S+ GVSCGS+VCDR+EN+GC++G CRYEV YGDGSYTKGTLALETLT  + ++
Sbjct: 188 FDPAKSGSYTGVSCGSSVCDRIENSGCHSGGCRYEVMYGDGSYTKGTLALETLTFAKTVV 247

Query: 251 RDVAIGCGHTNQGMFIGAAGLLGLGGGSMSFIGQLGGQTGGAFSYCLVSRGTGSTGTLEF 310
           R+VA+GCGH N+GMFIGAAGLLG+GGGSMSF+GQL GQTGGAF YCLVSRGT STG+L F
Sbjct: 248 RNVAMGCGHRNRGMFIGAAGLLGIGGGSMSFVGQLSGQTGGAFGYCLVSRGTDSTGSLVF 307

Query: 311 GRGTLPVGATWISLIRNPRAPSFYYIGLAGIGVGGVRVPVPEETFQLTEYGTNGVVMDTG 370
           GR  LPVGA+W+ L+RNPRAPSFYY+GL G+GVGGVR+P+P+  F LTE G  GVVMDTG
Sbjct: 308 GREALPVGASWVPLVRNPRAPSFYYVGLKGLGVGGVRIPLPDGVFDLTETGDGGVVMDTG 367

Query: 371 TAVTRLPTAAYVALRDSFTAQTSNLPRAPGVSIFDTCYDLNGFESVRVPTVSFYFSDGPV 430
           TAVTRLPTAAYVA RD F +QT+NLPRA GVSIFDTCYDL+GF SVRVPTVSFYF++GPV
Sbjct: 368 TAVTRLPTAAYVAFRDGFKSQTANLPRASGVSIFDTCYDLSGFVSVRVPTVSFYFTEGPV 427

Query: 431 LTLPAKNFLIPVDGGGTFCLAFAPSPSGLSIIGNIQQEGIQISFDGANGFVGFGPNIC 482
           LTLPA+NFL+PVD  GT+C AFA SP+GLSIIGNIQQEGIQ+SFDGANGFVGFGPN+C
Sbjct: 428 LTLPARNFLMPVDDSGTYCFAFAASPTGLSIIGNIQQEGIQVSFDGANGFVGFGPNVC 470

BLAST of Cla97C02G040000 vs. TAIR 10
Match: AT1G25510.1 (Eukaryotic aspartyl protease family protein )

HSP 1 Score: 397.9 bits (1021), Expect = 1.2e-110
Identity = 213/491 (43.38%), Postives = 299/491 (60.90%), Query Frame = 0

Query: 8   SLLFFLHLLFFSAAVAARPSPPTKFQYLNVKATKLDFNDGQILHTLNFSDGHR---QASG 67
           S  FF+  L   ++V +R  P T         T    N    +H   ++   R   Q   
Sbjct: 6   SFFFFIFFLTSHSSVFSRILPETS------TTTTSILNVADSIHRTKYTSSFRLNQQEEQ 65

Query: 68  HKSDNNTFKLNLVHRDKLSHVHGHRHGFNE-----RIKRDAIRVATLVRRLSHGGGAVQD 127
             S +++F L L  R     V G  H   +     R+ RD  RV +L+ RL      +  
Sbjct: 66  THSASSSFSLQLHSR---VSVRGTEHSDYKSLTLARLNRDTARVKSLITRLDLAINNISK 125

Query: 128 NKYK---------VANFGTDVISGMEEGSGEYFVRIGVGSPPRSQYMVIDSGSDIVWVQC 187
              K           +    +ISG  +GSGEYF R+G+G P R  YMV+D+GSD+ W+QC
Sbjct: 126 ADLKPISTMYTTEEQDIEAPLISGTTQGSGEYFTRVGIGKPAREVYMVLDTGSDVNWLQC 185

Query: 188 QPCTRCYQQSDPVFDPADSSSFAGVSCGSAVCDRLENTGCNAGRCRYEVSYGDGSYTKGT 247
            PC  CY Q++P+F+P+ SSS+  +SC +  C+ LE + C    C YEVSYGDGSYT G 
Sbjct: 186 TPCADCYHQTEPIFEPSSSSSYEPLSCDTPQCNALEVSECRNATCLYEVSYGDGSYTVGD 245

Query: 248 LALETLTLGQVMIRDVAIGCGHTNQGMFIGAAGLLGLGGGSMSFIGQLGGQTGGAFSYCL 307
            A ETLT+G  ++++VA+GCGH+N+G+F+GAAGLLGLGGG ++   QL      +FSYCL
Sbjct: 246 FATETLTIGSTLVQNVAVGCGHSNEGLFVGAAGLLGLGGGLLALPSQLNTT---SFSYCL 305

Query: 308 VSRGTGSTGTLEFGRGTLPVGATWISLIRNPRAPSFYYIGLAGIGVGGVRVPVPEETFQL 367
           V R + S  T++FG    P  A    L+RN +  +FYY+GL GI VGG  + +P+ +F++
Sbjct: 306 VDRDSDSASTVDFGTSLSP-DAVVAPLLRNHQLDTFYYLGLTGISVGGELLQIPQSSFEM 365

Query: 368 TEYGTNGVVMDTGTAVTRLPTAAYVALRDSFTAQTSNLPRAPGVSIFDTCYDLNGFESVR 427
            E G+ G+++D+GTAVTRL T  Y +LRDSF   T +L +A GV++FDTCY+L+   +V 
Sbjct: 366 DESGSGGIIIDSGTAVTRLQTEIYNSLRDSFVKGTLDLEKAAGVAMFDTCYNLSAKTTVE 425

Query: 428 VPTVSFYFSDGPVLTLPAKNFLIPVDGGGTFCLAFAPSPSGLSIIGNIQQEGIQISFDGA 482
           VPTV+F+F  G +L LPAKN++IPVD  GTFCLAFAP+ S L+IIGN+QQ+G +++FD A
Sbjct: 426 VPTVAFHFPGGKMLALPAKNYMIPVDSVGTFCLAFAPTASSLAIIGNVQQQGTRVTFDLA 483

BLAST of Cla97C02G040000 vs. TAIR 10
Match: AT1G01300.1 (Eukaryotic aspartyl protease family protein )

HSP 1 Score: 391.0 bits (1003), Expect = 1.4e-108
Identity = 218/484 (45.04%), Postives = 297/484 (61.36%), Query Frame = 0

Query: 8   SLLFFLHLLFFS-AAVAARPSPPTKF--QYLNVKATKLDF---NDGQILHTLNFSDGHRQ 67
           +LLF L   F S  + ++ PS  T F   +    A+ + F   +D + L    F  G   
Sbjct: 7   ALLFSLCFFFLSLPSFSSLPSFQTLFPNSHSLPCASPVSFQPDSDSESLLESEFESGSDS 66

Query: 68  ASGHKSDNNTFKLNLVHRDKLSHVHGHRHGFNERIKRDAIRVATLVRRLSHGGGAVQDNK 127
            S     +++  LNL H D LS        F+ R++RD+ RV ++    +   G    + 
Sbjct: 67  ES-----SSSITLNLDHIDALSSNKTPDELFSSRLQRDSRRVKSIATLAAQIPGRNVTHA 126

Query: 128 YKVANFGTDVISGMEEGSGEYFVRIGVGSPPRSQYMVIDSGSDIVWVQCQPCTRCYQQSD 187
            +   F + V+SG+ +GSGEYF R+GVG+P R  YMV+D+GSDIVW+QC PC RCY QSD
Sbjct: 127 PRPGGFSSSVVSGLSQGSGEYFTRLGVGTPARYVYMVLDTGSDIVWLQCAPCRRCYSQSD 186

Query: 188 PVFDPADSSSFAGVSCGSAVCDRLENTGCNAGR--CRYEVSYGDGSYTKGTLALETLTLG 247
           P+FDP  S ++A + C S  C RL++ GCN  R  C Y+VSYGDGS+T G  + ETLT  
Sbjct: 187 PIFDPRKSKTYATIPCSSPHCRRLDSAGCNTRRKTCLYQVSYGDGSFTVGDFSTETLTFR 246

Query: 248 QVMIRDVAIGCGHTNQGMFIGAAGLLGLGGGSMSFIGQLGGQTGGAFSYCLVSRGTGS-T 307
           +  ++ VA+GCGH N+G+F+GAAGLLGLG G +SF GQ G +    FSYCLV R   S  
Sbjct: 247 RNRVKGVALGCGHDNEGLFVGAAGLLGLGKGKLSFPGQTGHRFNQKFSYCLVDRSASSKP 306

Query: 308 GTLEFGRGTLPVGATWISLIRNPRAPSFYYIGLAGIGVGGVRVP-VPEETFQLTEYGTNG 367
            ++ FG   +   A +  L+ NP+  +FYY+GL GI VGG RVP V    F+L + G  G
Sbjct: 307 SSVVFGNAAVSRIARFTPLLSNPKLDTFYYVGLLGISVGGTRVPGVTASLFKLDQIGNGG 366

Query: 368 VVMDTGTAVTRLPTAAYVALRDSFTAQTSNLPRAPGVSIFDTCYDLNGFESVRVPTVSFY 427
           V++D+GT+VTRL   AY+A+RD+F      L RAP  S+FDTC+DL+    V+VPTV  +
Sbjct: 367 VIIDSGTSVTRLIRPAYIAMRDAFRVGAKTLKRAPDFSLFDTCFDLSNMNEVKVPTVVLH 426

Query: 428 FSDGPVLTLPAKNFLIPVDGGGTFCLAFAPSPSGLSIIGNIQQEGIQISFDGANGFVGFG 482
           F  G  ++LPA N+LIPVD  G FC AFA +  GLSIIGNIQQ+G ++ +D A+  VGF 
Sbjct: 427 FR-GADVSLPATNYLIPVDTNGKFCFAFAGTMGGLSIIGNIQQQGFRVVYDLASSRVGFA 484

BLAST of Cla97C02G040000 vs. TAIR 10
Match: AT3G18490.1 (Eukaryotic aspartyl protease family protein )

HSP 1 Score: 390.6 bits (1002), Expect = 1.9e-108
Identity = 190/399 (47.62%), Postives = 267/399 (66.92%), Query Frame = 0

Query: 95  RIKRDAIRVATLVRRLSHGGGAV----------QDNKYKVANFGTDVISGMEEGSGEYFV 154
           R++RD+ RVA +V ++      V          +D +Y+  +  T V+SG  +GSGEYF 
Sbjct: 105 RLERDSSRVAGIVAKIRFAVEGVDRSDLKPVYNEDTRYQTEDLTTPVVSGASQGSGEYFS 164

Query: 155 RIGVGSPPRSQYMVIDSGSDIVWVQCQPCTRCYQQSDPVFDPADSSSFAGVSCGSAVCDR 214
           RIGVG+P +  Y+V+D+GSD+ W+QC+PC  CYQQSDPVF+P  SS++  ++C +  C  
Sbjct: 165 RIGVGTPAKEMYLVLDTGSDVNWIQCEPCADCYQQSDPVFNPTSSSTYKSLTCSAPQCSL 224

Query: 215 LENTGCNAGRCRYEVSYGDGSYTKGTLALETLTLGQV-MIRDVAIGCGHTNQGMFIGAAG 274
           LE + C + +C Y+VSYGDGS+T G LA +T+T G    I +VA+GCGH N+G+F GAAG
Sbjct: 225 LETSACRSNKCLYQVSYGDGSFTVGELATDTVTFGNSGKINNVALGCGHDNEGLFTGAAG 284

Query: 275 LLGLGGGSMSFIGQLGGQTGGAFSYCLVSRGTGSTGTLEFGRGTLPVGATWISLIRNPRA 334
           LLGLGGG +S   Q+      +FSYCLV R +G + +L+F    L  G     L+RN + 
Sbjct: 285 LLGLGGGVLSITNQM---KATSFSYCLVDRDSGKSSSLDFNSVQLGGGDATAPLLRNKKI 344

Query: 335 PSFYYIGLAGIGVGGVRVPVPEETFQLTEYGTNGVVMDTGTAVTRLPTAAYVALRDSFTA 394
            +FYY+GL+G  VGG +V +P+  F +   G+ GV++D GTAVTRL T AY +LRD+F  
Sbjct: 345 DTFYYVGLSGFSVGGEKVVLPDAIFDVDASGSGGVILDCGTAVTRLQTQAYNSLRDAFLK 404

Query: 395 QTSNLPR-APGVSIFDTCYDLNGFESVRVPTVSFYFSDGPVLTLPAKNFLIPVDGGGTFC 454
            T NL + +  +S+FDTCYD +   +V+VPTV+F+F+ G  L LPAKN+LIPVD  GTFC
Sbjct: 405 LTVNLKKGSSSISLFDTCYDFSSLSTVKVPTVAFHFTGGKSLDLPAKNYLIPVDDSGTFC 464

Query: 455 LAFAPSPSGLSIIGNIQQEGIQISFDGANGFVGFGPNIC 482
            AFAP+ S LSIIGN+QQ+G +I++D +   +G   N C
Sbjct: 465 FAFAPTSSSLSIIGNVQQQGTRITYDLSKNVIGLSGNKC 500

BLAST of Cla97C02G040000 vs. TAIR 10
Match: AT3G61820.1 (Eukaryotic aspartyl protease family protein )

HSP 1 Score: 373.2 bits (957), Expect = 3.1e-103
Identity = 205/445 (46.07%), Postives = 279/445 (62.70%), Query Frame = 0

Query: 52  TLNFSDGHRQASGHKSDNNT-FKLNLVHRDKLSHVHGHRHG--FNERIKRDAIRV--ATL 111
           TL++ +         S++ T   ++L H D LS          FN R++RD++RV   T 
Sbjct: 40  TLSWPESESLTDESLSESTTSLSVHLSHVDALSSFSDASPADLFNLRLQRDSLRVKSITS 99

Query: 112 VRRLSHGGGAVQDNKYKVANFGTDVISGMEEGSGEYFVRIGVGSPPRSQYMVIDSGSDIV 171
           +  +S G  A +        F   VISG+ +GSGEYF+R+GVG+P  + YMV+D+GSD+V
Sbjct: 100 LAAVSTGRNATKRTPRTAGGFSGAVISGLSQGSGEYFMRLGVGTPATNVYMVLDTGSDVV 159

Query: 172 WVQCQPCTRCYQQSDPVFDPADSSSFAGVSCGSAVCDRLENTG-CNAGR---CRYEVSYG 231
           W+QC PC  CY Q+D +FDP  S +FA V CGS +C RL+++  C   R   C Y+VSYG
Sbjct: 160 WLQCSPCKACYNQTDAIFDPKKSKTFATVPCGSRLCRRLDDSSECVTRRSKTCLYQVSYG 219

Query: 232 DGSYTKGTLALETLTLGQVMIRDVAIGCGHTNQGMFIGAAGLLGLGGGSMSFIGQLGGQT 291
           DGS+T+G  + ETLT     +  V +GCGH N+G+F+GAAGLLGLG G +SF  Q   + 
Sbjct: 220 DGSFTEGDFSTETLTFHGARVDHVPLGCGHDNEGLFVGAAGLLGLGRGGLSFPSQTKNRY 279

Query: 292 GGAFSYCLVSR-GTGST----GTLEFGRGTLPVGATWISLIRNPRAPSFYYIGLAGIGVG 351
            G FSYCLV R  +GS+     T+ FG   +P  + +  L+ NP+  +FYY+ L GI VG
Sbjct: 280 NGKFSYCLVDRTSSGSSSKPPSTIVFGNAAVPKTSVFTPLLTNPKLDTFYYLQLLGISVG 339

Query: 352 GVRVP-VPEETFQLTEYGTNGVVMDTGTAVTRLPTAAYVALRDSFTAQTSNLPRAPGVSI 411
           G RVP V E  F+L   G  GV++D+GT+VTRL   AYVALRD+F    + L RAP  S+
Sbjct: 340 GSRVPGVSESQFKLDATGNGGVIIDSGTSVTRLTQPAYVALRDAFRLGATKLKRAPSYSL 399

Query: 412 FDTCYDLNGFESVRVPTVSFYFSDGPVLTLPAKNFLIPVDGGGTFCLAFAPSPSGLSIIG 471
           FDTC+DL+G  +V+VPTV F+F  G V +LPA N+LIPV+  G FC AFA +   LSIIG
Sbjct: 400 FDTCFDLSGMTTVKVPTVVFHFGGGEV-SLPASNYLIPVNTEGRFCFAFAGTMGSLSIIG 459

Query: 472 NIQQEGIQISFDGANGFVGFGPNIC 482
           NIQQ+G ++++D     VGF    C
Sbjct: 460 NIQQQGFRVAYDLVGSRVGFLSRAC 483

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
XP_038893071.12.1e-26394.80protein ASPARTIC PROTEASE IN GUARD CELL 2-like [Benincasa hispida][more]
XP_008445900.11.3e-25792.71PREDICTED: protein ASPARTIC PROTEASE IN GUARD CELL 2-like [Cucumis melo][more]
XP_004147103.15.2e-25491.29protein ASPARTIC PROTEASE IN GUARD CELL 2 [Cucumis sativus] >KGN51568.1 hypothet... [more]
KAA0034086.13.6e-24790.00protein ASPARTIC PROTEASE IN GUARD CELL 2-like [Cucumis melo var. makuwa][more]
XP_023547721.19.5e-24085.77protein ASPARTIC PROTEASE IN GUARD CELL 2-like [Cucurbita pepo subsp. pepo][more]
Match NameE-valueIdentityDescription
Q9LHE37.2e-18265.69Protein ASPARTIC PROTEASE IN GUARD CELL 2 OS=Arabidopsis thaliana OX=3702 GN=ASP... [more]
Q9LNJ32.0e-10745.04Aspartyl protease family protein 2 OS=Arabidopsis thaliana OX=3702 GN=APF2 PE=2 ... [more]
Q9LS402.6e-10747.62Protein ASPARTIC PROTEASE IN GUARD CELL 1 OS=Arabidopsis thaliana OX=3702 GN=ASP... [more]
Q8S9J66.1e-7239.52Aspartyl protease family protein At5g10770 OS=Arabidopsis thaliana OX=3702 GN=At... [more]
Q9LEW31.4e-6837.38Aspartyl protease AED1 OS=Arabidopsis thaliana OX=3702 GN=AED1 PE=2 SV=1[more]
Match NameE-valueIdentityDescription
A0A1S3BEG76.4e-25892.71protein ASPARTIC PROTEASE IN GUARD CELL 2-like OS=Cucumis melo OX=3656 GN=LOC103... [more]
A0A0A0KPR42.5e-25491.29Peptidase A1 domain-containing protein OS=Cucumis sativus OX=3659 GN=Csa_5G57906... [more]
A0A5A7SVY21.7e-24790.00Protein ASPARTIC PROTEASE IN GUARD CELL 2-like OS=Cucumis melo var. makuwa OX=11... [more]
A0A6J1KA061.9e-23885.60protein ASPARTIC PROTEASE IN GUARD CELL 2-like OS=Cucurbita maxima OX=3661 GN=LO... [more]
A0A6J1GXH55.2e-23684.05protein ASPARTIC PROTEASE IN GUARD CELL 2-like OS=Cucurbita moschata OX=3662 GN=... [more]
Match NameE-valueIdentityDescription
AT3G20015.15.1e-18365.69Eukaryotic aspartyl protease family protein [more]
AT1G25510.11.2e-11043.38Eukaryotic aspartyl protease family protein [more]
AT1G01300.11.4e-10845.04Eukaryotic aspartyl protease family protein [more]
AT3G18490.11.9e-10847.62Eukaryotic aspartyl protease family protein [more]
AT3G61820.13.1e-10346.07Eukaryotic aspartyl protease family protein [more]
InterPro
Analysis Name: InterPro Annotations of Watermelon (97103) v2.5
Date Performed: 2022-01-31
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR032799Xylanase inhibitor, C-terminalPFAMPF14541TAXi_Ccoord: 326..477
e-value: 2.2E-33
score: 115.3
IPR032861Xylanase inhibitor, N-terminalPFAMPF14543TAXi_Ncoord: 142..304
e-value: 1.3E-49
score: 168.8
IPR021109Aspartic peptidase domain superfamilyGENE3D2.40.70.10Acid Proteasescoord: 119..304
e-value: 7.2E-51
score: 174.9
IPR021109Aspartic peptidase domain superfamilyGENE3D2.40.70.10Acid Proteasescoord: 311..481
e-value: 7.8E-46
score: 158.0
IPR021109Aspartic peptidase domain superfamilySUPERFAMILY50630Acid proteasescoord: 138..481
NoneNo IPR availablePANTHERPTHR13683:SF838PROTEIN ASPARTIC PROTEASE IN GUARD CELL 2-LIKEcoord: 67..481
IPR001461Aspartic peptidase A1 familyPANTHERPTHR13683ASPARTYL PROTEASEScoord: 67..481
IPR033121Peptidase family A1 domainPROSITEPS51767PEPTIDASE_A1coord: 142..477
score: 45.459389
IPR033873CND41-likeCDDcd05472cnd41_likecoord: 141..481
e-value: 6.27833E-134
score: 387.012

Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
Cla97C02G040000.1Cla97C02G040000.1mRNA


GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
biological_process GO:0006508 proteolysis
molecular_function GO:0004190 aspartic-type endopeptidase activity