Cla97C02G034290 (gene) Watermelon (97103) v2.5

Overview
NameCla97C02G034290
Typegene
OrganismCitrullus lanatus subsp. vulgaris cv. 97103 (Watermelon (97103) v2.5)
Description4-coumarate-CoA ligase
LocationCla97Chr02: 8473865 .. 8480972 (+)
RNA-Seq ExpressionCla97C02G034290
SyntenyCla97C02G034290
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: exonCDSpolypeptide
Hold the cursor over a type above to highlight its positions in the sequence below.
ATGATTTCCATTGCCCAAAATCCCAATGTCTCCTCCGAACTCTCCTCCTCTCCCCCGCCTTCCACCGCCACAACCCATACTTTCCGATCCAAATTTCCCGATATTACTATCCCCGACCACCTCCCTCTTCACAGTTACTGCTTTCAGAACCTCTCTGAAGTTTCCCACCGCCCCTGTTTGATCCTCGGCTCCACTGGCAAATCCTATTCCTACTCCGACACCCACCTCTTCTCCCTCAAAGCCGCCGCCACTTTCTCTAAACTTGGAGTTAAGAAAGGCGATATCATTATGATTCTTCTCCACAACTCTCCCGAATTTGTTTTCTCTTTTATGGCTTCCTCCATGCTCGGCGCCGTCGCCACCACCGCAAATCCGTACTACACGGCGGCCGAGATTTCCAAGCAGTTGAAGGCCTCCGGTGCCAAATTCGTGGTTACTTACTCCCAATGCGTCGACAAGCTCAAAGAATCCGGCGAACATCTCACCATTGTCACCGTCGATGACCCGCCAGAGAATTGTCTGAGCTTCTCGATGGTGTACGACGCCGATGTGAATGACGTACCCTCCGTGGAGATTGACACAAACGACGCCGTTTCGCTACCGTTCTCCTCTGGCACGACCGGGCTCCCGAAGGGTGTGATTTTGACTCATAAGAACATGGTGTCGAGTGTGGCTCAACAGGTTCTCATAAATAAAAAGCGTTGAACTCAAAAGATAGATTATTATATATATTTAATCATATATTTTATATACTTCAAAATTAAGCTAATTGGAAAATATACAAGGGCCCAACAGATAGCTACCCATATTAAAAGACATGAGATAGAGGTATGTTTGGAGGTGACTTTAAATTTTATTTAAAAAAAAATATATATATATATACACACATATATAAAATCATTAAATTATGAATCTATGTGACTGACTAATGATATAATGTAATTACGGAAAGAAACTGAGTGGTTATTGGTTTATTTAATCATCTATTCATGTTATGAATGATGCAGGTGGATGGAGAGAATCCAAACTTGTATTTAAGAAGAAACGACGTAGTGCTATGCGTGTTACCAATGTTCCACATATTCTCGTTGAGTAGCATAGTTTTGATATCAATTAGGTCAGGGGCAGCGCTGCTTTTGATGGAGAAGTTCGAGATAGAGTCATTGCTAAGACTAATAGAGAGGCACGAGGTGACAGTGGCGGCGGTGGTGCCGCCGCTTGTGGTAGCGCTAGTGAAGAACCCGAGGTTGGCGGATTTTGACCTGAGCTCGATTAGATTGGTCCTTTCCGGAGCGGCGCCGCTGCGGAAGGAGCTGGAGGAGGCTCTCATGGAGAGGCTTCCTCAAGCCATTTTTGGTCAGGTATGGTAATGAAAATGGGTATAAATGGTATGGCGTGTTTGTTGTGAATATTTAAATAACAGGTTCCCAAAAATGTTATAGATCTAGCATGTGAAAGTAGTTAATAAAAAGGGTTTTATTTTGCAATCTCTTAGAAAATCAAACTAAAACAGTTATGTTATGCACTTTTGTAAGAACATGTGGATGGTAACTAAACAACAAAACTGGATTTCATATTATTCTTTTTCTCATATATTTCGGAAATCAAGTCAGATTCACCAATATTTGTACTACTCTAAATTTTTCCCTTCCTAAACTTTGTCACTTTTTTTTTAATTTGTATTAAATCCTAATATGCTTTTCTTTTTTATTTTATTATGGTTTTTAAAATCCCTTCGTCTAATTCCCCTACTTAACTAAACTTACTTACCTTTCTTCTTAAACTATTAAACTATTTGTTTTATTTTGAAAAAATATTTTTAGTTATTACTATTACTGTGTCTTTTTATATATAATTGTTGACTCCGTAAGACATTCAAATTTCAAATATATATAAACGTTAATATGATAATATACATTTTAAATATACTATGTAGATGGAACAATTGAACCTCTTACTTCAAGATAGATGGTAAAAACTAAAACAAAAAATTTCAAATTTCAAATACTAAAACAATAAGTGAATCATAATGTTAAAAATGCCCATTTGCCCAACTATAGGAACGGATTTAGAGGTGGTCCCTAGGTTTCCATTTCCATCGAAATAAATGCGTGTGTTTTCTAGGGGTTTTTTTTTTTTTTTTTTTTTTTCATTTAAATTGCTTATGTCCCTAAATTTTAGATTTAGTTTCCAAATATAGGTGGATGTGTTTTTTCTTCTCTTATATTTTTTTTAATCTTTAATTATTCCATATATATATATATATATAAACTTTTAGTACAACTTGAAATCATTTAGAACTTAGTTTGATTTTTAATTCGTTACAAATGTCCATATTTGGCAAAATATATATATATATATATATATATATATATATATATATTTTTAATGTAAAACTGTTGAAAATATTTACAAATAATAGTAAATATCACTGTTTATTTGCGATATACCGCAATAAATCACGATAAACTATTCTATATATCTATCATGACACAAATAGACGCTGATAATAATTTTTTTATTTATTTTTTATTTTTATATTTGAAAAATTCACATTTACTGCTATCAAGTTTCATTTTTTTTTCAATTATATATTTAGTGGAAAATTTAGAATGATTTATCAGAGTGTTGTAATAAAGATTAGTTAAAATAAATAGGGTTATGGGATGACGGAAGCGGGTCCCGTGCTGAGCATGTGCTCGGCTTTTGCAAAGGAGCCACTGATGCCAACAAAATCAGGTTCTTGTGGCAGAGTAGTGAGAAACTCGGAGCTCAAAGTCATTGACCCACAAACCGGCGACTCCCTCACTTATAATCAACCTGGAGAGATTTGTGTTCGAGGTCCTCAAGTAATGAAAGGTTCTTTCCTTCTTTCTTTATTTTTGTCTTTTCATTTTTCCATTTACACTATTTATTTTTTTCTTTTCATTTTTCATTTAGAATATTTATACACATGACAATTAAGATAATACCCATTAGATCAGTTTATTGATAAGAATCTATTATTGGATGATCTTGCTACATTTGTAATTTTTTTAAAAAATATTGCTATATATGTCATTATTAATTTTGAAGAGTAGTTTGTTTCGTAGGAATCCTTCAAGTCACCATTTAAATTTTTGCATTTGTTCTACGGTAACGTAAGATAATCTATATTTAGATGTTTTTATTTTTTAAAAATATGTTCAAATTTAAATTTAATTAGCATTTATTTTATGAAAAAAAATCTTATATTGATATATATATAATTTAGTGGAGAAAAATAACAAATAAAATAATAAAACTATATTAATTTGAAAAGAAATTCAAATTAAAATGTGTGATATAAGTTTAAATTAAATTAAATTATATTAATTATAAATCCATACAACTATTTTTGGAACATTAGGTAAAATTCAAACATTCTCAAGTAAAATCCTTCAAAACAAAAATTTATCTAAGTATTCTCAAATATTTTCAAAAATATTATTACAAAACAAACAAAGTTGTCTAACCATATATGATATATGTAATTCTGGAATCTATAATACTAGGTATATAAAGATTTTATTGATCTACATTCTCAAAAAAGAAATAAAGAGATGACCCCCTAAAAGTGGCATTCATTGTCATTACGTTTTTCCTTTTCACTAAATTAAATACCATATTGTTTGAAACTTTGACTAAATATCCAACAAAAAATTATCCAGTATAGTGGTAGTTGCAATTACGCACAAACTTTTAATTGTAAAAATTAGGTCTCTTAAACTTTTACAAACATTAAAATTGGACAAAACTTACAGTAGTTATAGAAATTGGACTTCCAAATGATAAAAATTGACACCTTAAATTTATACAATTGTTACAATTTCTACAATTATGTAAATTTCAGTGTTCAATTTCTACAATTGAAAGTTTGAGGGTATTATTGCAATTATCGCCATACTTTAGAGTTGGTTTTTGCGATTGGTTCTTAAACTATCAATTTTACTTTATAGACATAATTTAGAATTCTTTAGAAGTTATTTTCTCTTTTTATCATATAATAACTTGTTAAAATTCAACTTTTGTATGGTTTCATCAAAGGTATCCGGTTGGATTCTCTTTTTTAAGTAGGTGTTAGTAAACAAGGATAAAAAGTCAAGCCAAAATTTGAAAACTAACAAAAAAAAAAAAAAAATCTTTTAAAAATTTGTTTTTGTTTTTTTTTTTTTTGAAATTTGGTTAAAACTTCAACCATTGTGCTTAAGAAAGATGCAGAACATTGTAAGAAATGTGGAGAAATTATGCTTAATTTTCAAAAATCAAAAAACAAAAAATGAAATCATTACCAAACATAACTTAATTAATGAAAATTTATGGCTTTTGAAACTTGAGTTTTTAATTTCTTAAATAACTTAAATAGTATATCATATTAAAGAAATTTAAATTAAAGTAAAGTAGAAATTAGAAATTTGAATCTTCCCAACTCTAAAAGAGAAAATAATTTCAAATTCAATAATCTAGAAACTAATTAGGGTTAGGCAGAAGGTCCAAAAAGGAGTGATGTTTATTCATCTGCGCGCAACTGTCTTTTACCATAAAAATAAAGGTGAAAATTAAACCATCTTTGAAGCTGTTTAATTATGATTTAGAAAAGTTACAATAAATGGAATTTAATTTTGGTGCTAAACTGACCCGACTGTGATGTTAGGATATTTGAACGACCCGGTGTCCACGTCCTTGACTGTCGACGTGGAGGGTTGGCTTCATACCGGTGACATTGGTTACATTGACGATGAAGATGAAATTTTTATTGTTGACAGAGTCAAAGAGATCATTAAATTTAAAGGTTTTCAGGTACATGCACCTCACTTTTACTCTTACTTCATTACCAAAATATTAATATTTATATTATGTTTTAATCTTTTTCACTCATATTTAATTGTTATTTACTGAACTTGAAAAAGTCCAGAAGAATTTAATGATTTTTTTTTCTTTTTGAATAAAAAATGTTTATGGTCTTCAATTCTGAGTGTAATTTCTATTTGGATTTCAAAAACTTATAATATTCCTAATTTTGAGTATTTGATTTTAATAACGTGCAGTTTGATACTTTGATATTTGATGTCAAGTTTTAATTAATTGACTTAAAAAAGTTAAAATTTAAATTTAATAAAAAATGAATGGAAATTATTAGAGTGAGTTATTATAGTTACTTTCCAACTCTCTTGTATTCATGGCTATATATATGCCATCTTTGCACATAATAAATTAACGATTTTAATTCTTTTAATTCTTTAAAATTAATTGATAGACGTGAATATTTAACAAATAAAGGCCAACAGTGAGTACTTGTAAAAGATTAGGATTAAAAGTATTATTTTTTACGCAATTTGCATTATATATAATTTAAAACTCCAAATTTTATTTTTAATTGATTTTTCAGCCTTATAATTTTTTAGATAGGCAATAATCAAGTGCTATAGCTTAGCTGCACCTAATCACATACTCCGTTTTTAACACACAGAAAGAAAATTTTAAAAACCTTAACTTTTGGAAAAACAAAAAACTATTACGTGGCAGTGTTTTGCTCGACAACTTCCTATAAGGAGCACCGAAGTATTTTTCTTTTTAGATAATGAATTGTGTGCTATATTAAAATCAATTTTTTTTTTGGTCTACTCTAAAAAAATTAACAATCAAGGCTTCCTAACAACACTTGGTTTGTGTAACTAATTACAATTTGGTTTTGAATTATTTCTTGTTTTTTAAAAAATTAAATTGATATTAATGCAATTAAGTCTATAATTTATGATATTTTACAGGTGGCACCAGCAGAGTTAGAGGCCCTTCTTGTAACCCATCCATCTATTGTGGATGCCGCTGTTGTCCCGTAAGTGCACAATTGAAATTTCTTAGTTTTATAATTAAATTACATATAAATAATTCTTCCAAGGAAAAACATTTTTATTAACCGTAGCAAATGTAAACGTGTGTTTGTTAAATATTTTATTTGCATTTTTTAAAGCTTATTTAATATTAAAAAATTATACGTAAAAATTAAGATCTTGTGTGAGTTAGACCTCTTGTTTATTAGTGATTTTGTTTTTAGTTATATATATGGCATACATTTTCTTTTTTTCCTTTTTAGTTTATTTACTATTTTAATAAGCTTTTCAGTTTCCAAAATTAGATCTGCAATATAAGAAGTAATGTTTCTGAAAAATAACATTTTGAAAGACGACCAAAATCATTCTGAAAATGTTTTGATGAAAGTCGAGTAATCATTGAAAAGGGGGAAAAAAAAAAAAAAAAGGTGGAGTAACAGAGGAGAATTTGTTGGGCCAAAAATTCTTACTTTTCAAAACGAGAAAACTAAAAGTAAAATTATTTTGACGTTTTAAATTTTCTTTGGAATGATGAGAAAATAAACTGTTTGAGGAAATTACAGGCAAAGTGATGATGTTGCTGGTGAAGTTCCAGTGGCTTTCGTAGTTCCATCAACTCAGGATGAACTTACTGAGGACGCAGTAAAAGAATTCATAGCAAAGCAGGTTCGTGTATATTCTACCTAAATTTAATTTCAGTTGTAATCAAATTAGAATTATAATTCTAATTCGAGTTTAAATTTTTTATAATTATTCATTAATTTTCTACTAAATTATTCAATAAAAATTTATATTGCACGTATTCCTTTTTATTATGATTTTCACCTTAAAAATTGGTTTTTTTTTTTAGTGAATCCAATCCTTGCTTGTTCTCCTCATTATCAAATTGTTTTAAAATAAAAATCCTTATATTCATCTAATCAGTGTTACCCAAACGGAGTTAAAATTTTCATTTTTTTTTTTTTTTTAAATTTTGAAATCATTTAAAAAAGTACATAGTAAATAGAGTGGTGTTAAATCACAATAAAAAAGTTCAAATCAGTAGCCTGTATGAAGGGATGGAAAAGAAAATTTAGAAAAGCAAATACATGGATCTGACATTGCTTATTTTTTGTAAATTTGAATGCAAAACCAAACAGGTTGTGTTCTACAAGAGATTGCAGAAAGTGTATTTTGTGCAAACAATTCCAAAATCACCCTCTGGAAAGATTTTGAGAAAGGAACTCAAAACCAAGCTCTCAGTGTCACCTGGATTTTAG

mRNA sequence

ATGATTTCCATTGCCCAAAATCCCAATGTCTCCTCCGAACTCTCCTCCTCTCCCCCGCCTTCCACCGCCACAACCCATACTTTCCGATCCAAATTTCCCGATATTACTATCCCCGACCACCTCCCTCTTCACAGTTACTGCTTTCAGAACCTCTCTGAAGTTTCCCACCGCCCCTGTTTGATCCTCGGCTCCACTGGCAAATCCTATTCCTACTCCGACACCCACCTCTTCTCCCTCAAAGCCGCCGCCACTTTCTCTAAACTTGGAGTTAAGAAAGGCGATATCATTATGATTCTTCTCCACAACTCTCCCGAATTTGTTTTCTCTTTTATGGCTTCCTCCATGCTCGGCGCCGTCGCCACCACCGCAAATCCGTACTACACGGCGGCCGAGATTTCCAAGCAGTTGAAGGCCTCCGGTGCCAAATTCGTGGTTACTTACTCCCAATGCGTCGACAAGCTCAAAGAATCCGGCGAACATCTCACCATTGTCACCGTCGATGACCCGCCAGAGAATTGTCTGAGCTTCTCGATGGTGTACGACGCCGATGTGAATGACGTACCCTCCGTGGAGATTGACACAAACGACGCCGTTTCGCTACCGTTCTCCTCTGGCACGACCGGGCTCCCGAAGGGTGTGATTTTGACTCATAAGAACATGGTGTCGAGTGTGGCTCAACAGGTGGATGGAGAGAATCCAAACTTGTATTTAAGAAGAAACGACGTAGTGCTATGCGTGTTACCAATGTTCCACATATTCTCGTTGAGTAGCATAGTTTTGATATCAATTAGGTCAGGGGCAGCGCTGCTTTTGATGGAGAAGTTCGAGATAGAGTCATTGCTAAGACTAATAGAGAGGCACGAGGTGACAGTGGCGGCGGTGGTGCCGCCGCTTGTGGTAGCGCTAGTGAAGAACCCGAGGTTGGCGGATTTTGACCTGAGCTCGATTAGATTGGTCCTTTCCGGAGCGGCGCCGCTGCGGAAGGAGCTGGAGGAGGCTCTCATGGAGAGGCTTCCTCAAGCCATTTTTGGTCAGGGTTATGGGATGACGGAAGCGGGTCCCGTGCTGAGCATGTGCTCGGCTTTTGCAAAGGAGCCACTGATGCCAACAAAATCAGGTTCTTGTGGCAGAGTAGTGAGAAACTCGGAGCTCAAAGTCATTGACCCACAAACCGGCGACTCCCTCACTTATAATCAACCTGGAGAGATTTGTGTTCGAGGTCCTCAAGTAATGAAAGGATATTTGAACGACCCGGTGTCCACGTCCTTGACTGTCGACGTGGAGGGTTGGCTTCATACCGGTGACATTGGTTACATTGACGATGAAGATGAAATTTTTATTGTTGACAGAGTCAAAGAGATCATTAAATTTAAAGGTTTTCAGGTGGCACCAGCAGAGTTAGAGGCCCTTCTTGTAACCCATCCATCTATTGTGGATGCCGCTGTTGTCCCGCAAAGTGATGATGTTGCTGGTGAAGTTCCAGTGGCTTTCGTAGTTCCATCAACTCAGGATGAACTTACTGAGGACGCAGTAAAAGAATTCATAGCAAAGCAGGTTGTGTTCTACAAGAGATTGCAGAAAGTGTATTTTGTGCAAACAATTCCAAAATCACCCTCTGGAAAGATTTTGAGAAAGGAACTCAAAACCAAGCTCTCAGTGTCACCTGGATTTTAG

Coding sequence (CDS)

ATGATTTCCATTGCCCAAAATCCCAATGTCTCCTCCGAACTCTCCTCCTCTCCCCCGCCTTCCACCGCCACAACCCATACTTTCCGATCCAAATTTCCCGATATTACTATCCCCGACCACCTCCCTCTTCACAGTTACTGCTTTCAGAACCTCTCTGAAGTTTCCCACCGCCCCTGTTTGATCCTCGGCTCCACTGGCAAATCCTATTCCTACTCCGACACCCACCTCTTCTCCCTCAAAGCCGCCGCCACTTTCTCTAAACTTGGAGTTAAGAAAGGCGATATCATTATGATTCTTCTCCACAACTCTCCCGAATTTGTTTTCTCTTTTATGGCTTCCTCCATGCTCGGCGCCGTCGCCACCACCGCAAATCCGTACTACACGGCGGCCGAGATTTCCAAGCAGTTGAAGGCCTCCGGTGCCAAATTCGTGGTTACTTACTCCCAATGCGTCGACAAGCTCAAAGAATCCGGCGAACATCTCACCATTGTCACCGTCGATGACCCGCCAGAGAATTGTCTGAGCTTCTCGATGGTGTACGACGCCGATGTGAATGACGTACCCTCCGTGGAGATTGACACAAACGACGCCGTTTCGCTACCGTTCTCCTCTGGCACGACCGGGCTCCCGAAGGGTGTGATTTTGACTCATAAGAACATGGTGTCGAGTGTGGCTCAACAGGTGGATGGAGAGAATCCAAACTTGTATTTAAGAAGAAACGACGTAGTGCTATGCGTGTTACCAATGTTCCACATATTCTCGTTGAGTAGCATAGTTTTGATATCAATTAGGTCAGGGGCAGCGCTGCTTTTGATGGAGAAGTTCGAGATAGAGTCATTGCTAAGACTAATAGAGAGGCACGAGGTGACAGTGGCGGCGGTGGTGCCGCCGCTTGTGGTAGCGCTAGTGAAGAACCCGAGGTTGGCGGATTTTGACCTGAGCTCGATTAGATTGGTCCTTTCCGGAGCGGCGCCGCTGCGGAAGGAGCTGGAGGAGGCTCTCATGGAGAGGCTTCCTCAAGCCATTTTTGGTCAGGGTTATGGGATGACGGAAGCGGGTCCCGTGCTGAGCATGTGCTCGGCTTTTGCAAAGGAGCCACTGATGCCAACAAAATCAGGTTCTTGTGGCAGAGTAGTGAGAAACTCGGAGCTCAAAGTCATTGACCCACAAACCGGCGACTCCCTCACTTATAATCAACCTGGAGAGATTTGTGTTCGAGGTCCTCAAGTAATGAAAGGATATTTGAACGACCCGGTGTCCACGTCCTTGACTGTCGACGTGGAGGGTTGGCTTCATACCGGTGACATTGGTTACATTGACGATGAAGATGAAATTTTTATTGTTGACAGAGTCAAAGAGATCATTAAATTTAAAGGTTTTCAGGTGGCACCAGCAGAGTTAGAGGCCCTTCTTGTAACCCATCCATCTATTGTGGATGCCGCTGTTGTCCCGCAAAGTGATGATGTTGCTGGTGAAGTTCCAGTGGCTTTCGTAGTTCCATCAACTCAGGATGAACTTACTGAGGACGCAGTAAAAGAATTCATAGCAAAGCAGGTTGTGTTCTACAAGAGATTGCAGAAAGTGTATTTTGTGCAAACAATTCCAAAATCACCCTCTGGAAAGATTTTGAGAAAGGAACTCAAAACCAAGCTCTCAGTGTCACCTGGATTTTAG

Protein sequence

MISIAQNPNVSSELSSSPPPSTATTHTFRSKFPDITIPDHLPLHSYCFQNLSEVSHRPCLILGSTGKSYSYSDTHLFSLKAAATFSKLGVKKGDIIMILLHNSPEFVFSFMASSMLGAVATTANPYYTAAEISKQLKASGAKFVVTYSQCVDKLKESGEHLTIVTVDDPPENCLSFSMVYDADVNDVPSVEIDTNDAVSLPFSSGTTGLPKGVILTHKNMVSSVAQQVDGENPNLYLRRNDVVLCVLPMFHIFSLSSIVLISIRSGAALLLMEKFEIESLLRLIERHEVTVAAVVPPLVVALVKNPRLADFDLSSIRLVLSGAAPLRKELEEALMERLPQAIFGQGYGMTEAGPVLSMCSAFAKEPLMPTKSGSCGRVVRNSELKVIDPQTGDSLTYNQPGEICVRGPQVMKGYLNDPVSTSLTVDVEGWLHTGDIGYIDDEDEIFIVDRVKEIIKFKGFQVAPAELEALLVTHPSIVDAAVVPQSDDVAGEVPVAFVVPSTQDELTEDAVKEFIAKQVVFYKRLQKVYFVQTIPKSPSGKILRKELKTKLSVSPGF
Homology
BLAST of Cla97C02G034290 vs. NCBI nr
Match: XP_038901546.1 (4-coumarate--CoA ligase 3 isoform X1 [Benincasa hispida])

HSP 1 Score: 986.1 bits (2548), Expect = 1.2e-283
Identity = 509/553 (92.04%), Postives = 525/553 (94.94%), Query Frame = 0

Query: 5   AQNPNVSSELSSSPPPSTATTHTFRSKFPDITIPDHLPLHSYCFQNLSEVSHRPCLILGS 64
           AQ PNVSSELSSSPPP+ A TH FRSK PDITIPDHLPLHSYCFQ LSEV   PCLI+GS
Sbjct: 10  AQQPNVSSELSSSPPPAMAPTHIFRSKLPDITIPDHLPLHSYCFQKLSEVKDLPCLIVGS 69

Query: 65  TGKSYSYSDTHLFSLKAAATFSKLGVKKGDIIMILLHNSPEFVFSFMASSMLGAVATTAN 124
           TGKSYSYS+THLFS KAAATFSKLGVKKGD+IMILL NSPEF+FSFM SSMLG VATTAN
Sbjct: 70  TGKSYSYSETHLFSRKAAATFSKLGVKKGDVIMILLQNSPEFIFSFMGSSMLGTVATTAN 129

Query: 125 PYYTAAEISKQLKASGAKFVVTYSQCVDKLKESGEHLTIVTVDDPPENCLSFSMVYDADV 184
           PYYTAAEISKQL ASGAKFVVTYSQCV KL+ESGE LTIVTVDDPPENCLSFSMVYDAD 
Sbjct: 130 PYYTAAEISKQLMASGAKFVVTYSQCVAKLRESGEDLTIVTVDDPPENCLSFSMVYDADE 189

Query: 185 NDVPSVEIDTNDAVSLPFSSGTTGLPKGVILTHKNMVSSVAQQVDGENPNLYLRRNDVVL 244
           NDVP VEIDTNDAVSLPFSSGTTGLPKGVILTHKNMVSSVAQQVDGENPNLYLRRND+VL
Sbjct: 190 NDVPFVEIDTNDAVSLPFSSGTTGLPKGVILTHKNMVSSVAQQVDGENPNLYLRRNDIVL 249

Query: 245 CVLPMFHIFSLSSIVLISIRSGAALLLMEKFEIESLLRLIERHEVTVAAVVPPLVVALVK 304
           CVLPMFHIFSLSSIVL+SIRSGAALLLMEKFEIESLLRLIE+H VTVA VVPPLVVALVK
Sbjct: 250 CVLPMFHIFSLSSIVLVSIRSGAALLLMEKFEIESLLRLIEKHGVTVATVVPPLVVALVK 309

Query: 305 NPRLADFDLSSIRLVLSGAAPLRKELEEALMERLPQAIFGQGYGMTEAGPVLSMCSAFAK 364
           NPR ADFDLSSIR+VLSGAAPLRKELEEALM+RLPQAIFGQGYGMTEAGPVLSMCSAFAK
Sbjct: 310 NPRAADFDLSSIRMVLSGAAPLRKELEEALMQRLPQAIFGQGYGMTEAGPVLSMCSAFAK 369

Query: 365 EPLMPTKSGSCGRVVRNSELKVIDPQTGDSLTYNQPGEICVRGPQVMKGYLNDPVSTSLT 424
           EP MPTKSGSCGRVVRNS+LKVIDPQTG SL+YNQ GEIC+RGPQVMKGYLNDPVSTSLT
Sbjct: 370 EPPMPTKSGSCGRVVRNSDLKVIDPQTGASLSYNQSGEICLRGPQVMKGYLNDPVSTSLT 429

Query: 425 VDVEGWLHTGDIGYIDDEDEIFIVDRVKEIIKFKGFQVAPAELEALLVTHPSIVDAAVVP 484
           VDVEGWLHTGDIGYIDDEDEIFIVDRVKEIIKFKGFQVAPAELE LLVTHPSIVDAAVVP
Sbjct: 430 VDVEGWLHTGDIGYIDDEDEIFIVDRVKEIIKFKGFQVAPAELETLLVTHPSIVDAAVVP 489

Query: 485 QSDDVAGEVPVAFVVPSTQDELTEDAVKEFIAKQVVFYKRLQKVYFVQTIPKSPSGKILR 544
           Q+DDVAGEVPVAFVVPST +ELTEDAVKEFIAKQVVFYKRLQKVYFVQTIPKSPSGKILR
Sbjct: 490 QNDDVAGEVPVAFVVPSTHNELTEDAVKEFIAKQVVFYKRLQKVYFVQTIPKSPSGKILR 549

Query: 545 KELKTKLSVSPGF 558
           KELK KLSVSP F
Sbjct: 550 KELKAKLSVSPAF 562

BLAST of Cla97C02G034290 vs. NCBI nr
Match: XP_008462779.1 (PREDICTED: 4-coumarate--CoA ligase 2 [Cucumis melo] >KAA0042418.1 4-coumarate--CoA ligase 2 [Cucumis melo var. makuwa] >TYK01002.1 4-coumarate--CoA ligase 2 [Cucumis melo var. makuwa])

HSP 1 Score: 962.6 bits (2487), Expect = 1.5e-276
Identity = 498/558 (89.25%), Postives = 527/558 (94.44%), Query Frame = 0

Query: 1   MISIA-----QNP-NVSSELSSSPPPSTATTHTFRSKFPDITIPDHLPLHSYCFQNLSEV 60
           MIS+A     Q P NVSS+ SSSPPPST  TH FRSK PDI IPDHLPLHSYCFQ LS+V
Sbjct: 1   MISLAPVCDPQPPNNVSSDFSSSPPPSTPATHVFRSKLPDIPIPDHLPLHSYCFQKLSQV 60

Query: 61  SHRPCLILGSTGKSYSYSDTHLFSLKAAATFSKLGVKKGDIIMILLHNSPEFVFSFMASS 120
           S RPCLI+GSTGKSYSYS+THLFS KAAATFSKLGV++GD+IMILLHNSPEF+FSFM SS
Sbjct: 61  SDRPCLIVGSTGKSYSYSETHLFSRKAAATFSKLGVQRGDVIMILLHNSPEFIFSFMGSS 120

Query: 121 MLGAVATTANPYYTAAEISKQLKASGAKFVVTYSQCVDKLKESGEHLTIVTVDDPPENCL 180
           MLGA+ATTANPYYT AEISKQLKASGAKFVVTYS+CVDKL+E GE LTIVTVDDPPENCL
Sbjct: 121 MLGAIATTANPYYTGAEISKQLKASGAKFVVTYSRCVDKLRECGEVLTIVTVDDPPENCL 180

Query: 181 SFSMVYDADVNDVPSVEIDTNDAVSLPFSSGTTGLPKGVILTHKNMVSSVAQQVDGENPN 240
           SFSMVYDA+ NDVP VEIDTNDAVSLPFSSGTTGLPKGVILTHKNMVSSVAQQVDGENPN
Sbjct: 181 SFSMVYDANENDVPFVEIDTNDAVSLPFSSGTTGLPKGVILTHKNMVSSVAQQVDGENPN 240

Query: 241 LYLRRNDVVLCVLPMFHIFSLSSIVLISIRSGAALLLMEKFEIESLLRLIERHEVTVAAV 300
           LYL+RND+VLCVLPMFHIFSLSSIVLISIRSGAALLLMEKFEIESLLRL+ERH+VTVA V
Sbjct: 241 LYLKRNDIVLCVLPMFHIFSLSSIVLISIRSGAALLLMEKFEIESLLRLVERHKVTVATV 300

Query: 301 VPPLVVALVKNPRLADFDLSSIRLVLSGAAPLRKELEEALMERLPQAIFGQGYGMTEAGP 360
           VPPLVV+LVKNP++ADF+LSSIRLVLSGAAPLRKELEEALMERLPQAIFGQGYGMTEAGP
Sbjct: 301 VPPLVVSLVKNPKVADFNLSSIRLVLSGAAPLRKELEEALMERLPQAIFGQGYGMTEAGP 360

Query: 361 VLSMCSAFAKEPLMPTKSGSCGRVVRNSELKVIDPQTGDSLTYNQPGEICVRGPQVMKGY 420
           VLSMCSAFAKEP MPTKSGSCGRVVRNSELKV+DP TG SLTYNQPGEICVRGPQVMKGY
Sbjct: 361 VLSMCSAFAKEPPMPTKSGSCGRVVRNSELKVVDPLTGASLTYNQPGEICVRGPQVMKGY 420

Query: 421 LNDPVSTSLTVDVEGWLHTGDIGYIDDEDEIFIVDRVKEIIKFKGFQVAPAELEALLVTH 480
           LNDPVSTSLTVDVEGWLHTGDIGY+DDE+EIFIVDRVKEIIKFKGFQVAPAELEALLVTH
Sbjct: 421 LNDPVSTSLTVDVEGWLHTGDIGYVDDEEEIFIVDRVKEIIKFKGFQVAPAELEALLVTH 480

Query: 481 PSIVDAAVVPQSDDVAGEVPVAFVVPSTQDELTEDAVKEFIAKQVVFYKRLQKVYFVQTI 540
            SIVDAAVVPQ+DDVAGEVPVAFVVPSTQ+ LTE++VKEFIAKQVVFYKRL KVYFV+TI
Sbjct: 481 TSIVDAAVVPQNDDVAGEVPVAFVVPSTQNGLTEESVKEFIAKQVVFYKRLHKVYFVKTI 540

Query: 541 PKSPSGKILRKELKTKLS 553
           PKSPSGK LRKELK KLS
Sbjct: 541 PKSPSGKTLRKELKAKLS 558

BLAST of Cla97C02G034290 vs. NCBI nr
Match: XP_038901547.1 (4-coumarate--CoA ligase 3 isoform X2 [Benincasa hispida])

HSP 1 Score: 952.2 bits (2460), Expect = 2.0e-273
Identity = 497/553 (89.87%), Postives = 513/553 (92.77%), Query Frame = 0

Query: 5   AQNPNVSSELSSSPPPSTATTHTFRSKFPDITIPDHLPLHSYCFQNLSEVSHRPCLILGS 64
           AQ PNVSSELSSSPPP+ A TH FRSK PDITIPDHLPLHSYCFQ LSEV   PCLI+GS
Sbjct: 10  AQQPNVSSELSSSPPPAMAPTHIFRSKLPDITIPDHLPLHSYCFQKLSEVKDLPCLIVGS 69

Query: 65  TGKSYSYSDTHLFSLKAAATFSKLGVKKGDIIMILLHNSPEFVFSFMASSMLGAVATTAN 124
           TGKSYSYS+THLFS KAAATFSKLGVKKGD+IMILL NSPEF+FSFM SSMLG VATTAN
Sbjct: 70  TGKSYSYSETHLFSRKAAATFSKLGVKKGDVIMILLQNSPEFIFSFMGSSMLGTVATTAN 129

Query: 125 PYYTAAEISKQLKASGAKFVVTYSQCVDKLKESGEHLTIVTVDDPPENCLSFSMVYDADV 184
           PYYTAAEISKQL ASGAKFVVTYSQCV KL+ESGE LTIVTVDDPPENCLSFSMVYDAD 
Sbjct: 130 PYYTAAEISKQLMASGAKFVVTYSQCVAKLRESGEDLTIVTVDDPPENCLSFSMVYDADE 189

Query: 185 NDVPSVEIDTNDAVSLPFSSGTTGLPKGVILTHKNMVSSVAQQVDGENPNLYLRRNDVVL 244
           NDVP VEIDTNDAVSLPFSSGTTGLPKGVILTHKNMVSSVAQQVDGENPNLYLRRND+VL
Sbjct: 190 NDVPFVEIDTNDAVSLPFSSGTTGLPKGVILTHKNMVSSVAQQVDGENPNLYLRRNDIVL 249

Query: 245 CVLPMFHIFSLSSIVLISIRSGAALLLMEKFEIESLLRLIERHEVTVAAVVPPLVVALVK 304
           CVLPMFHIFSLSSIVL+SIRSGAALLLMEKFEIESLLRLIE+H VTVA VVPPLVVALVK
Sbjct: 250 CVLPMFHIFSLSSIVLVSIRSGAALLLMEKFEIESLLRLIEKHGVTVATVVPPLVVALVK 309

Query: 305 NPRLADFDLSSIRLVLSGAAPLRKELEEALMERLPQAIFGQGYGMTEAGPVLSMCSAFAK 364
           NPR ADFDLSSIR+VLSGAAPLRKELEEALM+RLPQAIFGQGYGMTEAGPVLSMCSAFAK
Sbjct: 310 NPRAADFDLSSIRMVLSGAAPLRKELEEALMQRLPQAIFGQGYGMTEAGPVLSMCSAFAK 369

Query: 365 EPLMPTKSGSCGRVVRNSELKVIDPQTGDSLTYNQPGEICVRGPQVMKGYLNDPVSTSLT 424
           EP MPTKSGSCGRVVRNS+LKVIDPQTG SL+YNQ GEIC+RGPQVMK            
Sbjct: 370 EPPMPTKSGSCGRVVRNSDLKVIDPQTGASLSYNQSGEICLRGPQVMK------------ 429

Query: 425 VDVEGWLHTGDIGYIDDEDEIFIVDRVKEIIKFKGFQVAPAELEALLVTHPSIVDAAVVP 484
           VDVEGWLHTGDIGYIDDEDEIFIVDRVKEIIKFKGFQVAPAELE LLVTHPSIVDAAVVP
Sbjct: 430 VDVEGWLHTGDIGYIDDEDEIFIVDRVKEIIKFKGFQVAPAELETLLVTHPSIVDAAVVP 489

Query: 485 QSDDVAGEVPVAFVVPSTQDELTEDAVKEFIAKQVVFYKRLQKVYFVQTIPKSPSGKILR 544
           Q+DDVAGEVPVAFVVPST +ELTEDAVKEFIAKQVVFYKRLQKVYFVQTIPKSPSGKILR
Sbjct: 490 QNDDVAGEVPVAFVVPSTHNELTEDAVKEFIAKQVVFYKRLQKVYFVQTIPKSPSGKILR 549

Query: 545 KELKTKLSVSPGF 558
           KELK KLSVSP F
Sbjct: 550 KELKAKLSVSPAF 550

BLAST of Cla97C02G034290 vs. NCBI nr
Match: XP_004151908.1 (4-coumarate--CoA ligase 2 [Cucumis sativus] >KAE8652343.1 hypothetical protein Csa_021810 [Cucumis sativus])

HSP 1 Score: 948.0 bits (2449), Expect = 3.7e-272
Identity = 488/557 (87.61%), Postives = 526/557 (94.43%), Query Frame = 0

Query: 1   MISIAQ--NPNVSSELSSSPPPSTATTHTFRSKFPDITIPDHLPLHSYCFQNLSEVSHRP 60
           MIS+A   +P   + +SSSPPPST  TH FRSK PDI IPDHLPLH+Y FQ LS+VS RP
Sbjct: 1   MISLAPLCDPQQPNNVSSSPPPSTPPTHVFRSKLPDIPIPDHLPLHTYSFQKLSQVSDRP 60

Query: 61  CLILGSTGKSYSYSDTHLFSLKAAATFSKLGVKKGDIIMILLHNSPEFVFSFMASSMLGA 120
           CLI+GSTGKSYSYS+THL S KAAATFSKLGVK+GD+IMILLHNSPEF+FSFM SSMLGA
Sbjct: 61  CLIVGSTGKSYSYSETHLLSRKAAATFSKLGVKRGDVIMILLHNSPEFIFSFMGSSMLGA 120

Query: 121 VATTANPYYTAAEISKQLKASGAKFVVTYSQCVDKLKES-GEHLTIVTVDDPPENCLSFS 180
           VATTANPYYT AEIS+QLKASGAKFVVTYS+CVDKL+ES G+ LTIVT+D PPENCLSFS
Sbjct: 121 VATTANPYYTGAEISRQLKASGAKFVVTYSRCVDKLRESCGDVLTIVTIDAPPENCLSFS 180

Query: 181 MVYDADVNDVPSVEIDTNDAVSLPFSSGTTGLPKGVILTHKNMVSSVAQQVDGENPNLYL 240
           MVYDAD NDVPSVEIDTNDAV+LPFSSGTTGLPKGVILTHKNMVSSVAQQVDGENPNLYL
Sbjct: 181 MVYDADENDVPSVEIDTNDAVALPFSSGTTGLPKGVILTHKNMVSSVAQQVDGENPNLYL 240

Query: 241 RRNDVVLCVLPMFHIFSLSSIVLISIRSGAALLLMEKFEIESLLRLIERHEVTVAAVVPP 300
           +RNDVVLCVLPMFHIFSLSSIVLIS+RSGAALLL+EKFEIESLLRL+ERH+VTVA VVPP
Sbjct: 241 KRNDVVLCVLPMFHIFSLSSIVLISMRSGAALLLIEKFEIESLLRLVERHKVTVATVVPP 300

Query: 301 LVVALVKNPRLADFDLSSIRLVLSGAAPLRKELEEALMERLPQAIFGQGYGMTEAGPVLS 360
           LVV+LVKNP++ADFDLSSIRLVLSGAAPLRKELEEALMERLPQAIFGQGYGMTEAGPVLS
Sbjct: 301 LVVSLVKNPKVADFDLSSIRLVLSGAAPLRKELEEALMERLPQAIFGQGYGMTEAGPVLS 360

Query: 361 MCSAFAKEPLMPTKSGSCGRVVRNSELKVIDPQTGDSLTYNQPGEICVRGPQVMKGYLND 420
           MCSAFAKEP MPTKSGSCGRVVRNSELKV+DP TG SLTYNQPGEICVRGPQVMKGYLND
Sbjct: 361 MCSAFAKEPPMPTKSGSCGRVVRNSELKVVDPITGASLTYNQPGEICVRGPQVMKGYLND 420

Query: 421 PVSTSLTVDVEGWLHTGDIGYIDDEDEIFIVDRVKEIIKFKGFQVAPAELEALLVTHPSI 480
           PVSTSLTVDVEGWLHTGDIG++DDE+EIFIVDRVKEIIKFKGFQVAPAELEALLVTH SI
Sbjct: 421 PVSTSLTVDVEGWLHTGDIGFVDDEEEIFIVDRVKEIIKFKGFQVAPAELEALLVTHTSI 480

Query: 481 VDAAVVPQSDDVAGEVPVAFVVPSTQDELTEDAVKEFIAKQVVFYKRLQKVYFVQTIPKS 540
           VDAAVVPQ+DDVAGEVPVAF+VPST +EL+E++VKEFIAKQVVFYKRL KVYFV+TIPKS
Sbjct: 481 VDAAVVPQNDDVAGEVPVAFIVPSTHNELSEESVKEFIAKQVVFYKRLHKVYFVKTIPKS 540

Query: 541 PSGKILRKELKTKLSVS 555
           PSGKILRKELK KLS++
Sbjct: 541 PSGKILRKELKAKLSIT 557

BLAST of Cla97C02G034290 vs. NCBI nr
Match: XP_022947959.1 (4-coumarate--CoA ligase 3 [Cucurbita moschata])

HSP 1 Score: 867.5 bits (2240), Expect = 6.4e-248
Identity = 441/556 (79.32%), Postives = 498/556 (89.57%), Query Frame = 0

Query: 1   MISIAQ-NPNVSSELSSSPPPSTATTHTFRSKFPDITIPDHLPLHSYCFQNLSEVSHRPC 60
           MIS+AQ +     +LSSSPPP       FRSK PDITIPDHLPLH YCF+ +SE S RPC
Sbjct: 1   MISVAQLSDGEKPKLSSSPPP-----RVFRSKLPDITIPDHLPLHDYCFEKISEFSDRPC 60

Query: 61  LILGSTGKSYSYSDTHLFSLKAAATFSKLGVKKGDIIMILLHNSPEFVFSFMASSMLGAV 120
           LI+G+TGKSYS+SDTHLFS +AAATFSKLGVKKGD IMILL NS EFVFSFM SSM+G+V
Sbjct: 61  LIVGATGKSYSFSDTHLFSKRAAATFSKLGVKKGDFIMILLKNSAEFVFSFMGSSMIGSV 120

Query: 121 ATTANPYYTAAEISKQLKASGAKFVVTYSQCVDKLKESGEHLTIVTVDDPPENCLSFSMV 180
           ATTANPYYTAAEISKQLK SGAK VVTYS CVDKL+ES   LTIVTVDDPPENCLSFSMV
Sbjct: 121 ATTANPYYTAAEISKQLKMSGAKLVVTYSHCVDKLRESDVDLTIVTVDDPPENCLSFSMV 180

Query: 181 YDADVNDVPSVEIDTNDAVSLPFSSGTTGLPKGVILTHKNMVSSVAQQVDGENPNLYLRR 240
           YDAD NDVP VEID NDAVSLPFSSGTTG PKGV+LTHK+MVSS+AQQVDGENPN+YL  
Sbjct: 181 YDADENDVPFVEIDWNDAVSLPFSSGTTGTPKGVVLTHKSMVSSIAQQVDGENPNIYLGT 240

Query: 241 NDVVLCVLPMFHIFSLSSIVLISIRSGAALLLMEKFEIESLLRLIERHEVTVAAVVPPLV 300
           NDVVLCVLPMFHIFSLSSIVLISIRSGA +LL+EKFEIE+ + LIERH VTVA VVPP+V
Sbjct: 241 NDVVLCVLPMFHIFSLSSIVLISIRSGATILLIEKFEIETSMSLIERHGVTVATVVPPMV 300

Query: 301 VALVKNPRLADFDLSSIRLVLSGAAPLRKELEEALMERLPQAIFGQGYGMTEAGPVLSMC 360
           + +VKNP++ADF+LSSIR+V+SGAAPL K++EEALMER+PQA+ GQGYGMTEAGPVLSMC
Sbjct: 301 LMMVKNPKVADFNLSSIRMVISGAAPLGKQIEEALMERIPQAVIGQGYGMTEAGPVLSMC 360

Query: 361 SAFAKEPLMPTKSGSCGRVVRNSELKVIDPQTGDSLTYNQPGEICVRGPQVMKGYLNDPV 420
           +AFAKEP MPTK GSCGRVVRNSELK+IDP+TG SLTYNQPGEIC+RGPQ+MKGYLNDPV
Sbjct: 361 TAFAKEPPMPTKIGSCGRVVRNSELKIIDPKTGVSLTYNQPGEICIRGPQIMKGYLNDPV 420

Query: 421 STSLTVDVEGWLHTGDIGYIDDEDEIFIVDRVKEIIKFKGFQVAPAELEALLVTHPSIVD 480
           +TSLTVDVEGWLHTGDIGYID+E+EIFIVDR+KEIIK+KGFQVAP ELE+LL+THPSI++
Sbjct: 421 ATSLTVDVEGWLHTGDIGYIDEEEEIFIVDRIKEIIKYKGFQVAPVELESLLLTHPSIME 480

Query: 481 AAVVPQSDDVAGEVPVAFVVPSTQDELTEDAVKEFIAKQVVFYKRLQKVYFVQTIPKSPS 540
            AVV ++D++AGEVPVAFVV S  ++LT++ VKEFIAKQVVFYKRL +VYFV TIPK PS
Sbjct: 481 VAVVAENDEIAGEVPVAFVVCSAGNQLTQEEVKEFIAKQVVFYKRLHRVYFVPTIPKLPS 540

Query: 541 GKILRKELKTKLSVSP 556
           GKIL+K+LK KLS SP
Sbjct: 541 GKILKKDLKAKLSNSP 551

BLAST of Cla97C02G034290 vs. ExPASy Swiss-Prot
Match: P31687 (4-coumarate--CoA ligase 2 OS=Glycine max OX=3847 PE=2 SV=2)

HSP 1 Score: 752.7 bits (1942), Expect = 3.0e-216
Identity = 377/536 (70.34%), Postives = 449/536 (83.77%), Query Frame = 0

Query: 24  TTHTFRSKFPDITIPDHLPLHSYCFQNLSEVSHRPCLILGSTGKSYSYSDTHLFSLKAAA 83
           T+H F+SK PDI I +HLPLHSYCFQNLS+ +HRPCLI+G   K+++Y+DTHL S K AA
Sbjct: 23  TSHVFKSKLPDIPISNHLPLHSYCFQNLSQFAHRPCLIVGPASKTFTYADTHLISSKIAA 82

Query: 84  TFSKLGVKKGDIIMILLHNSPEFVFSFMASSMLGAVATTANPYYTAAEISKQLKASGAKF 143
             S LG+ KGD++MILL NS +FVFSF+A SM+GAVATTANP+YTA EI KQ   S AK 
Sbjct: 83  GLSNLGILKGDVVMILLQNSADFVFSFLAISMIGAVATTANPFYTAPEIFKQFTVSKAKL 142

Query: 144 VVTYSQCVDKLK-----ESGEHLTIVTVDDPPENCLSFSMVYDADVNDVPSVEIDTNDAV 203
           ++T +  VDKL+     + GE   +VTVDDPPENCL FS++ +A+ +DVP VEI  +DAV
Sbjct: 143 IITQAMYVDKLRNHDGAKLGEDFKVVTVDDPPENCLHFSVLSEANESDVPEVEIHPDDAV 202

Query: 204 SLPFSSGTTGLPKGVILTHKNMVSSVAQQVDGENPNLYLRRNDVVLCVLPMFHIFSLSSI 263
           ++PFSSGTTGLPKGVILTHK++ +SVAQQVDGENPNLYL   DV+LCVLP+FHIFSL+S+
Sbjct: 203 AMPFSSGTTGLPKGVILTHKSLTTSVAQQVDGENPNLYLTTEDVLLCVLPLFHIFSLNSV 262

Query: 264 VLISIRSGAALLLMEKFEIESLLRLIERHEVTVAAVVPPLVVALVKNPRLADFDLSSIRL 323
           +L ++R+G+A+LLM+KFEI +LL LI+RH V+VA VVPPLV+AL KNP +ADFDLSSIRL
Sbjct: 263 LLCALRAGSAVLLMQKFEIGTLLELIQRHRVSVAMVVPPLVLALAKNPMVADFDLSSIRL 322

Query: 324 VLSGAAPLRKELEEALMERLPQAIFGQGYGMTEAGPVLSMCSAFAKEPLMPTKSGSCGRV 383
           VLSGAAPL KELEEAL  R+PQA+ GQGYGMTEAGPVLSMC  FAK+P   TKSGSCG V
Sbjct: 323 VLSGAAPLGKELEEALRNRMPQAVLGQGYGMTEAGPVLSMCLGFAKQPFQ-TKSGSCGTV 382

Query: 384 VRNSELKVIDPQTGDSLTYNQPGEICVRGPQVMKGYLNDPVSTSLTVDVEGWLHTGDIGY 443
           VRN+ELKV+DP+TG SL YNQPGEIC+RG Q+MKGYLND  +T+ T+D EGWLHTGD+GY
Sbjct: 383 VRNAELKVVDPETGRSLGYNQPGEICIRGQQIMKGYLNDEAATASTIDSEGWLHTGDVGY 442

Query: 444 IDDEDEIFIVDRVKEIIKFKGFQVAPAELEALLVTHPSIVDAAVVPQSDDVAGEVPVAFV 503
           +DD+DEIFIVDRVKE+IK+KGFQV PAELE LLV+HPSI DAAVVPQ D  AGEVPVAFV
Sbjct: 443 VDDDDEIFIVDRVKELIKYKGFQVPPAELEGLLVSHPSIADAAVVPQKDVAAGEVPVAFV 502

Query: 504 VPSTQDELTEDAVKEFIAKQVVFYKRLQKVYFVQTIPKSPSGKILRKELKTKLSVS 555
           V S   +LTE+AVKEFIAKQVVFYKRL KVYFV  IPKSPSGKILRK+L+ KL  +
Sbjct: 503 VRSNGFDLTEEAVKEFIAKQVVFYKRLHKVYFVHAIPKSPSGKILRKDLRAKLETA 557

BLAST of Cla97C02G034290 vs. ExPASy Swiss-Prot
Match: Q9S777 (4-coumarate--CoA ligase 3 OS=Arabidopsis thaliana OX=3702 GN=4CL3 PE=1 SV=1)

HSP 1 Score: 731.5 bits (1887), Expect = 7.2e-210
Identity = 367/536 (68.47%), Postives = 452/536 (84.33%), Query Frame = 0

Query: 19  PPSTATTHTFRSKFPDITIPDHLPLHSYCFQNLSEVSHRPCLILGSTGKSYSYSDTHLFS 78
           P S  T   FRSK PDI IP+HLPLH+YCF+ LS VS +PCLI+GSTGKSY+Y +THL  
Sbjct: 26  PHSPPTPRIFRSKLPDIDIPNHLPLHTYCFEKLSSVSDKPCLIVGSTGKSYTYGETHLIC 85

Query: 79  LKAAATFSKLGVKKGDIIMILLHNSPEFVFSFMASSMLGAVATTANPYYTAAEISKQLKA 138
            + A+   KLG++KGD+IMILL NS EFVFSFM +SM+GAV+TTANP+YT+ E+ KQLK+
Sbjct: 86  RRVASGLYKLGIRKGDVIMILLQNSAEFVFSFMGASMIGAVSTTANPFYTSQELYKQLKS 145

Query: 139 SGAKFVVTYSQCVDKLKESGEHLTIVTVDDP-PENCLSFS-MVYDADVNDV-PSVEIDTN 198
           SGAK ++T+SQ VDKLK  GE+LT++T D+P PENCL FS ++ D + N    +V+I  +
Sbjct: 146 SGAKLIITHSQYVDKLKNLGENLTLITTDEPTPENCLPFSTLITDDETNPFQETVDIGGD 205

Query: 199 DAVSLPFSSGTTGLPKGVILTHKNMVSSVAQQVDGENPNLYLRRNDVVLCVLPMFHIFSL 258
           DA +LPFSSGTTGLPKGV+LTHK++++SVAQQVDG+NPNLYL+ NDV+LCVLP+FHI+SL
Sbjct: 206 DAAALPFSSGTTGLPKGVVLTHKSLITSVAQQVDGDNPNLYLKSNDVILCVLPLFHIYSL 265

Query: 259 SSIVLISIRSGAALLLMEKFEIESLLRLIERHEVTVAAVVPPLVVALVKNPRLADFDLSS 318
           +S++L S+RSGA +LLM KFEI +LL LI+RH VT+AA+VPPLV+AL KNP +  +DLSS
Sbjct: 266 NSVLLNSLRSGATVLLMHKFEIGALLDLIQRHRVTIAALVPPLVIALAKNPTVNSYDLSS 325

Query: 319 IRLVLSGAAPLRKELEEALMERLPQAIFGQGYGMTEAGPVLSMCSAFAKEPLMPTKSGSC 378
           +R VLSGAAPL KEL+++L  RLPQAI GQGYGMTEAGPVLSM   FAKEP+ PTKSGSC
Sbjct: 326 VRFVLSGAAPLGKELQDSLRRRLPQAILGQGYGMTEAGPVLSMSLGFAKEPI-PTKSGSC 385

Query: 379 GRVVRNSELKVIDPQTGDSLTYNQPGEICVRGPQVMKGYLNDPVSTSLTVDVEGWLHTGD 438
           G VVRN+ELKV+  +T  SL YNQPGEIC+RG Q+MK YLNDP +TS T+D EGWLHTGD
Sbjct: 386 GTVVRNAELKVVHLETRLSLGYNQPGEICIRGQQIMKEYLNDPEATSATIDEEGWLHTGD 445

Query: 439 IGYIDDEDEIFIVDRVKEIIKFKGFQVAPAELEALLVTHPSIVDAAVVPQSDDVAGEVPV 498
           IGY+D++DEIFIVDR+KE+IKFKGFQV PAELE+LL+ H SI DAAVVPQ+D+VAGEVPV
Sbjct: 446 IGYVDEDDEIFIVDRLKEVIKFKGFQVPPAELESLLINHHSIADAAVVPQNDEVAGEVPV 505

Query: 499 AFVVPSTQDELTEDAVKEFIAKQVVFYKRLQKVYFVQTIPKSPSGKILRKELKTKL 552
           AFVV S  +++TE+ VKE++AKQVVFYKRL KV+FV +IPKSPSGKILRK+LK KL
Sbjct: 506 AFVVRSNGNDITEEDVKEYVAKQVVFYKRLHKVFFVASIPKSPSGKILRKDLKAKL 560

BLAST of Cla97C02G034290 vs. ExPASy Swiss-Prot
Match: Q42982 (4-coumarate--CoA ligase 2 OS=Oryza sativa subsp. japonica OX=39947 GN=4CL2 PE=1 SV=2)

HSP 1 Score: 682.6 bits (1760), Expect = 3.8e-195
Identity = 354/571 (62.00%), Postives = 436/571 (76.36%), Query Frame = 0

Query: 1   MISIA---QNPNVSSELSSSPPPSTATTHTFRSKFPDITIPDHLPLHSYCFQNLSEVSHR 60
           MI++A     P V++ +  +PP +      FRSK PDI IP HLPLH YCF   +E+   
Sbjct: 1   MITVAAPEAQPQVAAAVDEAPPEAVT---VFRSKLPDIDIPSHLPLHEYCFARAAELPDA 60

Query: 61  PCLILGSTGKSYSYSDTHLFSLKAAATFSKLGVKKGDIIMILLHNSPEFVFSFMASSMLG 120
           PCLI  +TG++Y++++T L   +AAA   +LGV  GD +M+LL N  EF  +F A+S LG
Sbjct: 61  PCLIAAATGRTYTFAETRLLCRRAAAALHRLGVGHGDRVMVLLQNCVEFAVAFFAASFLG 120

Query: 121 AVATTANPYYTAAEISKQLKASGAKFVVTYSQCVDKLKE------------SGEHLTIVT 180
           AV T ANP+ T  EI KQ KASG K ++T S  VDKL++              + LT++T
Sbjct: 121 AVTTAANPFCTPQEIHKQFKASGVKLILTQSVYVDKLRQHEAFPRIDACTVGDDTLTVIT 180

Query: 181 VDD---PPENCLSF-SMVYDADVNDVPSVEIDTNDAVSLPFSSGTTGLPKGVILTHKNMV 240
           +DD    PE CL F  ++ DAD   VP V I  +D V+LPFSSGTTGLPKGV+LTH+++V
Sbjct: 181 IDDDEATPEGCLPFWDLIADADEGSVPEVAISPDDPVALPFSSGTTGLPKGVVLTHRSVV 240

Query: 241 SSVAQQVDGENPNLYLRRNDVVLCVLPMFHIFSLSSIVLISIRSGAALLLMEKFEIESLL 300
           S VAQQVDGENPNL++   DV LCVLP+FHIFSL+S++L ++R+GAA+ LM +FE+ ++L
Sbjct: 241 SGVAQQVDGENPNLHMGAGDVALCVLPLFHIFSLNSVLLCAVRAGAAVALMPRFEMGAML 300

Query: 301 RLIERHEVTVAAVVPPLVVALVKNPRLADFDLSSIRLVLSGAAPLRKELEEALMERLPQA 360
             IER  VTVAAVVPPLV+AL KNP +   DLSSIR+VLSGAAPL KELE+AL  RLPQA
Sbjct: 301 GAIERWRVTVAAVVPPLVLALAKNPFVERHDLSSIRIVLSGAAPLGKELEDALRARLPQA 360

Query: 361 IFGQGYGMTEAGPVLSMCSAFAKEPLMPTKSGSCGRVVRNSELKVIDPQTGDSLTYNQPG 420
           IFGQGYGMTEAGPVLSMC AFAKEP  P KSGSCG VVRN+ELKV+DP TG SL  N PG
Sbjct: 361 IFGQGYGMTEAGPVLSMCPAFAKEP-TPAKSGSCGTVVRNAELKVVDPDTGFSLGRNLPG 420

Query: 421 EICVRGPQVMKGYLNDPVSTSLTVDVEGWLHTGDIGYIDDEDEIFIVDRVKEIIKFKGFQ 480
           EIC+RGPQ+MKGYLNDP +T+ T+DVEGWLHTGDIGY+DD+DE+FIVDRVKE+IKFKGFQ
Sbjct: 421 EICIRGPQIMKGYLNDPEATAATIDVEGWLHTGDIGYVDDDDEVFIVDRVKELIKFKGFQ 480

Query: 481 VAPAELEALLVTHPSIVDAAVVPQSDDVAGEVPVAFVVPSTQDELTEDAVKEFIAKQVVF 540
           V PAELE+LL+ HPSI DAAVVPQ DDVAGEVPVAFVV +   ++TE+++KEFI+KQVVF
Sbjct: 481 VPPAELESLLIAHPSIADAAVVPQKDDVAGEVPVAFVVRAADSDITEESIKEFISKQVVF 540

Query: 541 YKRLQKVYFVQTIPKSPSGKILRKELKTKLS 553
           YKRL KV+F+  IPKS SGKILR+EL+ KL+
Sbjct: 541 YKRLHKVHFIHAIPKSASGKILRRELRAKLA 567

BLAST of Cla97C02G034290 vs. ExPASy Swiss-Prot
Match: A0A2H5AIY4 (4-coumarate-CoA ligase 2 OS=Narcissus pseudonarcissus OX=39639 GN=4CL2 PE=2 SV=1)

HSP 1 Score: 675.6 bits (1742), Expect = 4.7e-193
Identity = 354/567 (62.43%), Postives = 443/567 (78.13%), Query Frame = 0

Query: 1   MISIAQ-NPNVSSELSSSPPPSTATTHT-FRSKFPDITIPDHLPLHSYCFQNLSEVSHRP 60
           MI+IA+ +P +      +  PST  T T FRS+ PDI + +HLPLH Y F+N +  S  P
Sbjct: 1   MITIAESHPQIHHSPPDTTAPSTPPTATIFRSRLPDIVLSNHLPLHEYLFEN-TLTSPSP 60

Query: 61  CLILGSTGKSYSYSDTHLFSLKAAATF-SKLGVKKGDIIMILLHNSPEFVFSFMASSMLG 120
           C+I  STG+SYS+++THL S K A+   S+ GV++G ++M+LLHN PEFVFSF+ SSMLG
Sbjct: 61  CIISASTGRSYSFAETHLLSRKTASFLSSRCGVRRGGVVMLLLHNCPEFVFSFLGSSMLG 120

Query: 121 AVATTANPYYTAAEISKQLKASGAKFVVTYSQCVDKLK--ESGEHLTIVTVDD------- 180
           AV T ANP+ T  EI KQL ASGA  ++T S    K+   +  E L +VTV D       
Sbjct: 121 AVTTAANPFCTPQEIKKQLLASGATVIITQSAYASKISRDDDVEDLIVVTVSDNELERHA 180

Query: 181 -PPENCLSFSMVYDADVNDVP-SVEIDTNDAVSLPFSSGTTGLPKGVILTHKNMVSSVAQ 240
            PPE C+SFS V  AD + VP +V +   DAV++PFSSGTTGLPKGV+LTHK+M SSV Q
Sbjct: 181 APPEGCVSFSEVESADEDAVPDAVGVGPEDAVAMPFSSGTTGLPKGVVLTHKSMTSSVGQ 240

Query: 241 QVDGENPNLYLRR-NDVVLCVLPMFHIFSLSSIVLISIRSGAALLLMEKFEIESLLRLIE 300
            VDGENPNL+LR+  DV+LCVLP+FHIFSL+S++L  +R+GAA+++M +FE+E +L  I+
Sbjct: 241 IVDGENPNLHLRKGEDVLLCVLPLFHIFSLNSVLLCGLRTGAAVVIMARFEMEGMLETIQ 300

Query: 301 RHEVTVAAVVPPLVVALVKNPRLADFDLSSIRLVLSGAAPLRKELEEALMERLPQAIFGQ 360
           R  V+VAAVVPPLV+AL KNP +  +D+ ++R+VLSGAAPL KELE  L  RLPQA+ GQ
Sbjct: 301 RWGVSVAAVVPPLVLALAKNPLVEKYDMGTVRMVLSGAAPLGKELEAVLKGRLPQAVLGQ 360

Query: 361 GYGMTEAGPVLSMCSAFAKEPLMPTKSGSCGRVVRNSELKVIDPQTGDSLTYNQPGEICV 420
           GYGMTEAGPV+SM   FAK+P  P KSGSCG VVRN+ELKV+DP+TG SL  NQPGEICV
Sbjct: 361 GYGMTEAGPVISMSPGFAKQP-TPVKSGSCGTVVRNAELKVMDPETGFSLGRNQPGEICV 420

Query: 421 RGPQVMKGYLNDPVSTSLTVDVEGWLHTGDIGYIDDEDEIFIVDRVKEIIKFKGFQVAPA 480
           RGPQ+MKGYLNDP +TS T+DVEGWLHTGD+GY+DD+DE+FIVDRVKE+IKFKGFQV PA
Sbjct: 421 RGPQIMKGYLNDPEATSATIDVEGWLHTGDVGYVDDDDEVFIVDRVKELIKFKGFQVPPA 480

Query: 481 ELEALLVTHPSIVDAAVVPQSDDVAGEVPVAFVVPSTQDELTEDAVKEFIAKQVVFYKRL 540
           ELEALL+ HPSI DAAV+PQ+D+VAGEVPVAFVVPS   +LTE+ VKEFI+KQVVFYKR+
Sbjct: 481 ELEALLLGHPSIADAAVIPQNDEVAGEVPVAFVVPSKSSDLTEEIVKEFISKQVVFYKRI 540

Query: 541 QKVYFVQTIPKSPSGKILRKELKTKLS 553
            +VYF+  IPKSPSGKILRK+L+ K++
Sbjct: 541 HRVYFIHAIPKSPSGKILRKDLRAKVA 565

BLAST of Cla97C02G034290 vs. ExPASy Swiss-Prot
Match: I3PB37 (4-coumarate:CoA ligase 1 OS=Petunia hybrida OX=4102 GN=4CL1 PE=1 SV=1)

HSP 1 Score: 665.6 bits (1716), Expect = 4.9e-190
Identity = 323/527 (61.29%), Postives = 425/527 (80.65%), Query Frame = 0

Query: 28  FRSKFPDITIPDHLPLHSYCFQNLSEVSHRPCLILGSTGKSYSYSDTHLFSLKAAATFSK 87
           FRSK PDI IP HLPLHSYCF+N+SE S RPCLI G+    Y+Y+D  L S K AA  +K
Sbjct: 14  FRSKLPDIYIPKHLPLHSYCFENISEFSSRPCLINGANNHIYTYADVELTSRKVAAGLNK 73

Query: 88  LGVKKGDIIMILLHNSPEFVFSFMASSMLGAVATTANPYYTAAEISKQLKASGAKFVVTY 147
           LG+++ D IMILL NSPEFVF+FM +S LGA++T ANP +T AE+ KQ KAS AK ++T 
Sbjct: 74  LGIQQKDTIMILLPNSPEFVFAFMGASYLGAISTMANPLFTPAEVVKQAKASNAKLIITQ 133

Query: 148 SQCVDKLKESG--EHLTIVTVDDPPENCLSFSMVYDADVNDVPSVEIDTNDAVSLPFSSG 207
           +  V+K+K+     +L ++ +D  PE C+ FS +  AD +D+P V+I ++D V+LP+SSG
Sbjct: 134 ACFVNKVKDYAFDNNLNVICIDSAPEGCIHFSELTQADEHDIPDVKIQSDDVVALPYSSG 193

Query: 208 TTGLPKGVILTHKNMVSSVAQQVDGENPNLYLRRNDVVLCVLPMFHIFSLSSIVLISIRS 267
           TTGLPKGV+LTHK +V+SVAQQVDGEN NLY+   DV++CVLP+FHI+SL+S++L  +R 
Sbjct: 194 TTGLPKGVMLTHKGLVTSVAQQVDGENANLYMHSEDVLMCVLPLFHIYSLNSVLLCGLRV 253

Query: 268 GAALLLMEKFEIESLLRLIERHEVTVAAVVPPLVVALVKNPRLADFDLSSIRLVLSGAAP 327
           GAA+L+M+KF+I     LIE+++VT+   VPP+V+A+ K+P + ++DLSS+R V+SGAAP
Sbjct: 254 GAAILIMQKFDIVQFCELIEKYKVTIGPFVPPIVLAIAKSPVVDNYDLSSVRTVMSGAAP 313

Query: 328 LRKELEEALMERLPQAIFGQGYGMTEAGPVLSMCSAFAKEPLMPTKSGSCGRVVRNSELK 387
           L KELE+A+  + P A  GQGYGMTEAGPVL+MC AFAKEP    KSG+CG VVRN+E+K
Sbjct: 314 LGKELEDAVRIKFPNAKLGQGYGMTEAGPVLAMCLAFAKEP-FDIKSGACGTVVRNAEMK 373

Query: 388 VIDPQTGDSLTYNQPGEICVRGPQVMKGYLNDPVSTSLTVDVEGWLHTGDIGYIDDEDEI 447
           ++DP TG SL  NQPGEIC+RG Q+MKGYLNDP +T+ T+D EGWLHTGDIGYID++DE+
Sbjct: 374 IVDPDTGCSLPRNQPGEICIRGDQIMKGYLNDPAATTRTIDKEGWLHTGDIGYIDNDDEL 433

Query: 448 FIVDRVKEIIKFKGFQVAPAELEALLVTHPSIVDAAVVPQSDDVAGEVPVAFVVPSTQDE 507
           FIVDR+KE+IK+KGFQVAPAELEALL+ HP+I DAAVVP  D+ AGEVPVAFVV S   +
Sbjct: 434 FIVDRLKELIKYKGFQVAPAELEALLLNHPNISDAAVVPMKDEQAGEVPVAFVVRSNGSD 493

Query: 508 LTEDAVKEFIAKQVVFYKRLQKVYFVQTIPKSPSGKILRKELKTKLS 553
           +TED VK+F++KQV+FYKR+++V+FV+T+PKSPSGKILRK+L+ +L+
Sbjct: 494 ITEDEVKDFVSKQVIFYKRIKRVFFVETVPKSPSGKILRKDLRARLA 539

BLAST of Cla97C02G034290 vs. ExPASy TrEMBL
Match: A0A5A7THZ8 (4-coumarate--CoA ligase 2 OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold602G002030 PE=4 SV=1)

HSP 1 Score: 962.6 bits (2487), Expect = 7.1e-277
Identity = 498/558 (89.25%), Postives = 527/558 (94.44%), Query Frame = 0

Query: 1   MISIA-----QNP-NVSSELSSSPPPSTATTHTFRSKFPDITIPDHLPLHSYCFQNLSEV 60
           MIS+A     Q P NVSS+ SSSPPPST  TH FRSK PDI IPDHLPLHSYCFQ LS+V
Sbjct: 1   MISLAPVCDPQPPNNVSSDFSSSPPPSTPATHVFRSKLPDIPIPDHLPLHSYCFQKLSQV 60

Query: 61  SHRPCLILGSTGKSYSYSDTHLFSLKAAATFSKLGVKKGDIIMILLHNSPEFVFSFMASS 120
           S RPCLI+GSTGKSYSYS+THLFS KAAATFSKLGV++GD+IMILLHNSPEF+FSFM SS
Sbjct: 61  SDRPCLIVGSTGKSYSYSETHLFSRKAAATFSKLGVQRGDVIMILLHNSPEFIFSFMGSS 120

Query: 121 MLGAVATTANPYYTAAEISKQLKASGAKFVVTYSQCVDKLKESGEHLTIVTVDDPPENCL 180
           MLGA+ATTANPYYT AEISKQLKASGAKFVVTYS+CVDKL+E GE LTIVTVDDPPENCL
Sbjct: 121 MLGAIATTANPYYTGAEISKQLKASGAKFVVTYSRCVDKLRECGEVLTIVTVDDPPENCL 180

Query: 181 SFSMVYDADVNDVPSVEIDTNDAVSLPFSSGTTGLPKGVILTHKNMVSSVAQQVDGENPN 240
           SFSMVYDA+ NDVP VEIDTNDAVSLPFSSGTTGLPKGVILTHKNMVSSVAQQVDGENPN
Sbjct: 181 SFSMVYDANENDVPFVEIDTNDAVSLPFSSGTTGLPKGVILTHKNMVSSVAQQVDGENPN 240

Query: 241 LYLRRNDVVLCVLPMFHIFSLSSIVLISIRSGAALLLMEKFEIESLLRLIERHEVTVAAV 300
           LYL+RND+VLCVLPMFHIFSLSSIVLISIRSGAALLLMEKFEIESLLRL+ERH+VTVA V
Sbjct: 241 LYLKRNDIVLCVLPMFHIFSLSSIVLISIRSGAALLLMEKFEIESLLRLVERHKVTVATV 300

Query: 301 VPPLVVALVKNPRLADFDLSSIRLVLSGAAPLRKELEEALMERLPQAIFGQGYGMTEAGP 360
           VPPLVV+LVKNP++ADF+LSSIRLVLSGAAPLRKELEEALMERLPQAIFGQGYGMTEAGP
Sbjct: 301 VPPLVVSLVKNPKVADFNLSSIRLVLSGAAPLRKELEEALMERLPQAIFGQGYGMTEAGP 360

Query: 361 VLSMCSAFAKEPLMPTKSGSCGRVVRNSELKVIDPQTGDSLTYNQPGEICVRGPQVMKGY 420
           VLSMCSAFAKEP MPTKSGSCGRVVRNSELKV+DP TG SLTYNQPGEICVRGPQVMKGY
Sbjct: 361 VLSMCSAFAKEPPMPTKSGSCGRVVRNSELKVVDPLTGASLTYNQPGEICVRGPQVMKGY 420

Query: 421 LNDPVSTSLTVDVEGWLHTGDIGYIDDEDEIFIVDRVKEIIKFKGFQVAPAELEALLVTH 480
           LNDPVSTSLTVDVEGWLHTGDIGY+DDE+EIFIVDRVKEIIKFKGFQVAPAELEALLVTH
Sbjct: 421 LNDPVSTSLTVDVEGWLHTGDIGYVDDEEEIFIVDRVKEIIKFKGFQVAPAELEALLVTH 480

Query: 481 PSIVDAAVVPQSDDVAGEVPVAFVVPSTQDELTEDAVKEFIAKQVVFYKRLQKVYFVQTI 540
            SIVDAAVVPQ+DDVAGEVPVAFVVPSTQ+ LTE++VKEFIAKQVVFYKRL KVYFV+TI
Sbjct: 481 TSIVDAAVVPQNDDVAGEVPVAFVVPSTQNGLTEESVKEFIAKQVVFYKRLHKVYFVKTI 540

Query: 541 PKSPSGKILRKELKTKLS 553
           PKSPSGK LRKELK KLS
Sbjct: 541 PKSPSGKTLRKELKAKLS 558

BLAST of Cla97C02G034290 vs. ExPASy TrEMBL
Match: A0A1S3CJB3 (4-coumarate--CoA ligase 2 OS=Cucumis melo OX=3656 GN=LOC103501067 PE=4 SV=1)

HSP 1 Score: 962.6 bits (2487), Expect = 7.1e-277
Identity = 498/558 (89.25%), Postives = 527/558 (94.44%), Query Frame = 0

Query: 1   MISIA-----QNP-NVSSELSSSPPPSTATTHTFRSKFPDITIPDHLPLHSYCFQNLSEV 60
           MIS+A     Q P NVSS+ SSSPPPST  TH FRSK PDI IPDHLPLHSYCFQ LS+V
Sbjct: 1   MISLAPVCDPQPPNNVSSDFSSSPPPSTPATHVFRSKLPDIPIPDHLPLHSYCFQKLSQV 60

Query: 61  SHRPCLILGSTGKSYSYSDTHLFSLKAAATFSKLGVKKGDIIMILLHNSPEFVFSFMASS 120
           S RPCLI+GSTGKSYSYS+THLFS KAAATFSKLGV++GD+IMILLHNSPEF+FSFM SS
Sbjct: 61  SDRPCLIVGSTGKSYSYSETHLFSRKAAATFSKLGVQRGDVIMILLHNSPEFIFSFMGSS 120

Query: 121 MLGAVATTANPYYTAAEISKQLKASGAKFVVTYSQCVDKLKESGEHLTIVTVDDPPENCL 180
           MLGA+ATTANPYYT AEISKQLKASGAKFVVTYS+CVDKL+E GE LTIVTVDDPPENCL
Sbjct: 121 MLGAIATTANPYYTGAEISKQLKASGAKFVVTYSRCVDKLRECGEVLTIVTVDDPPENCL 180

Query: 181 SFSMVYDADVNDVPSVEIDTNDAVSLPFSSGTTGLPKGVILTHKNMVSSVAQQVDGENPN 240
           SFSMVYDA+ NDVP VEIDTNDAVSLPFSSGTTGLPKGVILTHKNMVSSVAQQVDGENPN
Sbjct: 181 SFSMVYDANENDVPFVEIDTNDAVSLPFSSGTTGLPKGVILTHKNMVSSVAQQVDGENPN 240

Query: 241 LYLRRNDVVLCVLPMFHIFSLSSIVLISIRSGAALLLMEKFEIESLLRLIERHEVTVAAV 300
           LYL+RND+VLCVLPMFHIFSLSSIVLISIRSGAALLLMEKFEIESLLRL+ERH+VTVA V
Sbjct: 241 LYLKRNDIVLCVLPMFHIFSLSSIVLISIRSGAALLLMEKFEIESLLRLVERHKVTVATV 300

Query: 301 VPPLVVALVKNPRLADFDLSSIRLVLSGAAPLRKELEEALMERLPQAIFGQGYGMTEAGP 360
           VPPLVV+LVKNP++ADF+LSSIRLVLSGAAPLRKELEEALMERLPQAIFGQGYGMTEAGP
Sbjct: 301 VPPLVVSLVKNPKVADFNLSSIRLVLSGAAPLRKELEEALMERLPQAIFGQGYGMTEAGP 360

Query: 361 VLSMCSAFAKEPLMPTKSGSCGRVVRNSELKVIDPQTGDSLTYNQPGEICVRGPQVMKGY 420
           VLSMCSAFAKEP MPTKSGSCGRVVRNSELKV+DP TG SLTYNQPGEICVRGPQVMKGY
Sbjct: 361 VLSMCSAFAKEPPMPTKSGSCGRVVRNSELKVVDPLTGASLTYNQPGEICVRGPQVMKGY 420

Query: 421 LNDPVSTSLTVDVEGWLHTGDIGYIDDEDEIFIVDRVKEIIKFKGFQVAPAELEALLVTH 480
           LNDPVSTSLTVDVEGWLHTGDIGY+DDE+EIFIVDRVKEIIKFKGFQVAPAELEALLVTH
Sbjct: 421 LNDPVSTSLTVDVEGWLHTGDIGYVDDEEEIFIVDRVKEIIKFKGFQVAPAELEALLVTH 480

Query: 481 PSIVDAAVVPQSDDVAGEVPVAFVVPSTQDELTEDAVKEFIAKQVVFYKRLQKVYFVQTI 540
            SIVDAAVVPQ+DDVAGEVPVAFVVPSTQ+ LTE++VKEFIAKQVVFYKRL KVYFV+TI
Sbjct: 481 TSIVDAAVVPQNDDVAGEVPVAFVVPSTQNGLTEESVKEFIAKQVVFYKRLHKVYFVKTI 540

Query: 541 PKSPSGKILRKELKTKLS 553
           PKSPSGK LRKELK KLS
Sbjct: 541 PKSPSGKTLRKELKAKLS 558

BLAST of Cla97C02G034290 vs. ExPASy TrEMBL
Match: A0A6J1G8F9 (4-coumarate--CoA ligase 3 OS=Cucurbita moschata OX=3662 GN=LOC111451685 PE=4 SV=1)

HSP 1 Score: 867.5 bits (2240), Expect = 3.1e-248
Identity = 441/556 (79.32%), Postives = 498/556 (89.57%), Query Frame = 0

Query: 1   MISIAQ-NPNVSSELSSSPPPSTATTHTFRSKFPDITIPDHLPLHSYCFQNLSEVSHRPC 60
           MIS+AQ +     +LSSSPPP       FRSK PDITIPDHLPLH YCF+ +SE S RPC
Sbjct: 1   MISVAQLSDGEKPKLSSSPPP-----RVFRSKLPDITIPDHLPLHDYCFEKISEFSDRPC 60

Query: 61  LILGSTGKSYSYSDTHLFSLKAAATFSKLGVKKGDIIMILLHNSPEFVFSFMASSMLGAV 120
           LI+G+TGKSYS+SDTHLFS +AAATFSKLGVKKGD IMILL NS EFVFSFM SSM+G+V
Sbjct: 61  LIVGATGKSYSFSDTHLFSKRAAATFSKLGVKKGDFIMILLKNSAEFVFSFMGSSMIGSV 120

Query: 121 ATTANPYYTAAEISKQLKASGAKFVVTYSQCVDKLKESGEHLTIVTVDDPPENCLSFSMV 180
           ATTANPYYTAAEISKQLK SGAK VVTYS CVDKL+ES   LTIVTVDDPPENCLSFSMV
Sbjct: 121 ATTANPYYTAAEISKQLKMSGAKLVVTYSHCVDKLRESDVDLTIVTVDDPPENCLSFSMV 180

Query: 181 YDADVNDVPSVEIDTNDAVSLPFSSGTTGLPKGVILTHKNMVSSVAQQVDGENPNLYLRR 240
           YDAD NDVP VEID NDAVSLPFSSGTTG PKGV+LTHK+MVSS+AQQVDGENPN+YL  
Sbjct: 181 YDADENDVPFVEIDWNDAVSLPFSSGTTGTPKGVVLTHKSMVSSIAQQVDGENPNIYLGT 240

Query: 241 NDVVLCVLPMFHIFSLSSIVLISIRSGAALLLMEKFEIESLLRLIERHEVTVAAVVPPLV 300
           NDVVLCVLPMFHIFSLSSIVLISIRSGA +LL+EKFEIE+ + LIERH VTVA VVPP+V
Sbjct: 241 NDVVLCVLPMFHIFSLSSIVLISIRSGATILLIEKFEIETSMSLIERHGVTVATVVPPMV 300

Query: 301 VALVKNPRLADFDLSSIRLVLSGAAPLRKELEEALMERLPQAIFGQGYGMTEAGPVLSMC 360
           + +VKNP++ADF+LSSIR+V+SGAAPL K++EEALMER+PQA+ GQGYGMTEAGPVLSMC
Sbjct: 301 LMMVKNPKVADFNLSSIRMVISGAAPLGKQIEEALMERIPQAVIGQGYGMTEAGPVLSMC 360

Query: 361 SAFAKEPLMPTKSGSCGRVVRNSELKVIDPQTGDSLTYNQPGEICVRGPQVMKGYLNDPV 420
           +AFAKEP MPTK GSCGRVVRNSELK+IDP+TG SLTYNQPGEIC+RGPQ+MKGYLNDPV
Sbjct: 361 TAFAKEPPMPTKIGSCGRVVRNSELKIIDPKTGVSLTYNQPGEICIRGPQIMKGYLNDPV 420

Query: 421 STSLTVDVEGWLHTGDIGYIDDEDEIFIVDRVKEIIKFKGFQVAPAELEALLVTHPSIVD 480
           +TSLTVDVEGWLHTGDIGYID+E+EIFIVDR+KEIIK+KGFQVAP ELE+LL+THPSI++
Sbjct: 421 ATSLTVDVEGWLHTGDIGYIDEEEEIFIVDRIKEIIKYKGFQVAPVELESLLLTHPSIME 480

Query: 481 AAVVPQSDDVAGEVPVAFVVPSTQDELTEDAVKEFIAKQVVFYKRLQKVYFVQTIPKSPS 540
            AVV ++D++AGEVPVAFVV S  ++LT++ VKEFIAKQVVFYKRL +VYFV TIPK PS
Sbjct: 481 VAVVAENDEIAGEVPVAFVVCSAGNQLTQEEVKEFIAKQVVFYKRLHRVYFVPTIPKLPS 540

Query: 541 GKILRKELKTKLSVSP 556
           GKIL+K+LK KLS SP
Sbjct: 541 GKILKKDLKAKLSNSP 551

BLAST of Cla97C02G034290 vs. ExPASy TrEMBL
Match: A0A0A0LQV1 (AMP-binding domain-containing protein OS=Cucumis sativus OX=3659 GN=Csa_2G433350 PE=4 SV=1)

HSP 1 Score: 835.9 bits (2158), Expect = 1.0e-238
Identity = 430/487 (88.30%), Postives = 459/487 (94.25%), Query Frame = 0

Query: 1   MISIAQ--NPNVSSELSSSPPPSTATTHTFRSKFPDITIPDHLPLHSYCFQNLSEVSHRP 60
           MIS+A   +P   + +SSSPPPST  TH FRSK PDI IPDHLPLH+Y FQ LS+VS RP
Sbjct: 1   MISLAPLCDPQQPNNVSSSPPPSTPPTHVFRSKLPDIPIPDHLPLHTYSFQKLSQVSDRP 60

Query: 61  CLILGSTGKSYSYSDTHLFSLKAAATFSKLGVKKGDIIMILLHNSPEFVFSFMASSMLGA 120
           CLI+GSTGKSYSYS+THL S KAAATFSKLGVK+GD+IMILLHNSPEF+FSFM SSMLGA
Sbjct: 61  CLIVGSTGKSYSYSETHLLSRKAAATFSKLGVKRGDVIMILLHNSPEFIFSFMGSSMLGA 120

Query: 121 VATTANPYYTAAEISKQLKASGAKFVVTYSQCVDKLKES-GEHLTIVTVDDPPENCLSFS 180
           VATTANPYYT AEIS+QLKASGAKFVVTYS+CVDKL+ES G+ LTIVT+D PPENCLSFS
Sbjct: 121 VATTANPYYTGAEISRQLKASGAKFVVTYSRCVDKLRESCGDVLTIVTIDAPPENCLSFS 180

Query: 181 MVYDADVNDVPSVEIDTNDAVSLPFSSGTTGLPKGVILTHKNMVSSVAQQVDGENPNLYL 240
           MVYDAD NDVPSVEIDTNDAV+LPFSSGTTGLPKGVILTHKNMVSSVAQQVDGENPNLYL
Sbjct: 181 MVYDADENDVPSVEIDTNDAVALPFSSGTTGLPKGVILTHKNMVSSVAQQVDGENPNLYL 240

Query: 241 RRNDVVLCVLPMFHIFSLSSIVLISIRSGAALLLMEKFEIESLLRLIERHEVTVAAVVPP 300
           +RNDVVLCVLPMFHIFSLSSIVLIS+RSGAALLL+EKFEIESLLRL+ERH+VTVA VVPP
Sbjct: 241 KRNDVVLCVLPMFHIFSLSSIVLISMRSGAALLLIEKFEIESLLRLVERHKVTVATVVPP 300

Query: 301 LVVALVKNPRLADFDLSSIRLVLSGAAPLRKELEEALMERLPQAIFGQGYGMTEAGPVLS 360
           LVV+LVKNP++ADFDLSSIRLVLSGAAPLRKELEEALMERLPQAIFGQGYGMTEAGPVLS
Sbjct: 301 LVVSLVKNPKVADFDLSSIRLVLSGAAPLRKELEEALMERLPQAIFGQGYGMTEAGPVLS 360

Query: 361 MCSAFAKEPLMPTKSGSCGRVVRNSELKVIDPQTGDSLTYNQPGEICVRGPQVMKGYLND 420
           MCSAFAKEP MPTKSGSCGRVVRNSELKV+DP TG SLTYNQPGEICVRGPQVMKGYLND
Sbjct: 361 MCSAFAKEPPMPTKSGSCGRVVRNSELKVVDPITGASLTYNQPGEICVRGPQVMKGYLND 420

Query: 421 PVSTSLTVDVEGWLHTGDIGYIDDEDEIFIVDRVKEIIKFKGFQVAPAELEALLVTHPSI 480
           PVSTSLTVDVEGWLHTGDIG++DDE+EIFIVDRVKEIIKFKGFQVAPAELEALLVTH SI
Sbjct: 421 PVSTSLTVDVEGWLHTGDIGFVDDEEEIFIVDRVKEIIKFKGFQVAPAELEALLVTHTSI 480

Query: 481 VDAAVVP 485
           VDAAVVP
Sbjct: 481 VDAAVVP 487

BLAST of Cla97C02G034290 vs. ExPASy TrEMBL
Match: A0A4Y1R2Z5 (4-coumarate:CoA ligase 3 OS=Prunus dulcis OX=3755 GN=ALMOND_2B015040 PE=4 SV=1)

HSP 1 Score: 774.2 bits (1998), Expect = 3.6e-220
Identity = 391/547 (71.48%), Postives = 459/547 (83.91%), Query Frame = 0

Query: 21  STATTHTFRSKFPDITIPDHLPLHSYCFQNLSEVSHRPCLILGSTGKSYSYSDTHLFSLK 80
           S  T H F+SK PDI IPDHLPLH+YCFQ LSE S RPCLI+GSTGKSYS+SDT+L S K
Sbjct: 55  SNTTNHVFKSKLPDIPIPDHLPLHTYCFQKLSEFSDRPCLIVGSTGKSYSFSDTYLISQK 114

Query: 81  AAATFSKLGVKKGDIIMILLHNSPEFVFSFMASSMLGAVATTANPYYTAAEISKQLKASG 140
             A  S LG+KKGD+IMILL N  EFVF+FM +SM+GAV TTANP+YTAAEI KQ KAS 
Sbjct: 115 TGAGLSNLGIKKGDVIMILLQNCAEFVFAFMGASMIGAVTTTANPFYTAAEIFKQFKASN 174

Query: 141 AKFVVTYSQCVDKLKES---------------GEHLTIVTVDDPPENCLSFSMVYDADVN 200
           AK ++T+SQ VDKL+E+               GEH  +VTVDDPPENCL FS++ +A+  
Sbjct: 175 AKLIITHSQFVDKLREAPADGSAPYQNNYPKLGEHFKVVTVDDPPENCLHFSVISEANET 234

Query: 201 DVPSVEIDTNDAVSLPFSSGTTGLPKGVILTHKNMVSSVAQQVDGENPNLYLRRNDVVLC 260
           ++P V ID  D V+LPFSSGTTGLPKGVILTHK++++SVAQQVDGENPNLYL+ +DVVLC
Sbjct: 235 ELPEVVIDPEDPVALPFSSGTTGLPKGVILTHKSLITSVAQQVDGENPNLYLKEDDVVLC 294

Query: 261 VLPMFHIFSLSSIVLISIRSGAALLLMEKFEIESLLRLIERHEVTVAAVVPPLVVALVKN 320
           VLP+FHI+SL+S++L S+R+GAA+L+M KFEI +LL LI+R+ V+VAAVVPPLV+AL KN
Sbjct: 295 VLPLFHIYSLNSVMLCSLRAGAAVLVMHKFEIGTLLELIQRYRVSVAAVVPPLVIALAKN 354

Query: 321 PRLADFDLSSIRLVLSGAAPLRKELEEALMERLPQAIFGQGYGMTEAGPVLSMCSAFAKE 380
           P +A FDLSSIR+VLSGAAPL KELEEAL  R+PQA+ GQGYGMTEAGPVLSMC AFAKE
Sbjct: 355 PMVAQFDLSSIRVVLSGAAPLGKELEEALKSRVPQAVLGQGYGMTEAGPVLSMCLAFAKE 414

Query: 381 PLMPTKSGSCGRVVRNSELKVIDPQTGDSLTYNQPGEICVRGPQVMKGYLNDPVSTSLTV 440
           PL PTKSGSCG VVRN+ELKVID +TG SL YNQPGEIC+RG Q+MKGYLND  +T+ TV
Sbjct: 415 PL-PTKSGSCGTVVRNAELKVIDAETGCSLGYNQPGEICIRGSQIMKGYLNDAEATATTV 474

Query: 441 DVEGWLHTGDIGYIDDEDEIFIVDRVKEIIKFKGFQVAPAELEALLVTHPSIVDAAVVPQ 500
           D EGWLHTGD+GY+DD++E+FIVDRVKE+IKFKGFQV PAELE+LLV+HPSI DAAVVPQ
Sbjct: 475 DKEGWLHTGDVGYVDDDEEVFIVDRVKELIKFKGFQVPPAELESLLVSHPSIADAAVVPQ 534

Query: 501 SDDVAGEVPVAFVVPSTQDELTEDAVKEFIAKQVVFYKRLQKVYFVQTIPKSPSGKILRK 553
            D+ AGEVPVAFVV S   +LTE+AVKEFIAKQVVFYKRL KVYFV  IPKSPSGKILRK
Sbjct: 535 KDEAAGEVPVAFVVRSNGLQLTEEAVKEFIAKQVVFYKRLHKVYFVHAIPKSPSGKILRK 594

BLAST of Cla97C02G034290 vs. TAIR 10
Match: AT1G65060.1 (4-coumarate:CoA ligase 3 )

HSP 1 Score: 731.5 bits (1887), Expect = 5.1e-211
Identity = 367/536 (68.47%), Postives = 452/536 (84.33%), Query Frame = 0

Query: 19  PPSTATTHTFRSKFPDITIPDHLPLHSYCFQNLSEVSHRPCLILGSTGKSYSYSDTHLFS 78
           P S  T   FRSK PDI IP+HLPLH+YCF+ LS VS +PCLI+GSTGKSY+Y +THL  
Sbjct: 26  PHSPPTPRIFRSKLPDIDIPNHLPLHTYCFEKLSSVSDKPCLIVGSTGKSYTYGETHLIC 85

Query: 79  LKAAATFSKLGVKKGDIIMILLHNSPEFVFSFMASSMLGAVATTANPYYTAAEISKQLKA 138
            + A+   KLG++KGD+IMILL NS EFVFSFM +SM+GAV+TTANP+YT+ E+ KQLK+
Sbjct: 86  RRVASGLYKLGIRKGDVIMILLQNSAEFVFSFMGASMIGAVSTTANPFYTSQELYKQLKS 145

Query: 139 SGAKFVVTYSQCVDKLKESGEHLTIVTVDDP-PENCLSFS-MVYDADVNDV-PSVEIDTN 198
           SGAK ++T+SQ VDKLK  GE+LT++T D+P PENCL FS ++ D + N    +V+I  +
Sbjct: 146 SGAKLIITHSQYVDKLKNLGENLTLITTDEPTPENCLPFSTLITDDETNPFQETVDIGGD 205

Query: 199 DAVSLPFSSGTTGLPKGVILTHKNMVSSVAQQVDGENPNLYLRRNDVVLCVLPMFHIFSL 258
           DA +LPFSSGTTGLPKGV+LTHK++++SVAQQVDG+NPNLYL+ NDV+LCVLP+FHI+SL
Sbjct: 206 DAAALPFSSGTTGLPKGVVLTHKSLITSVAQQVDGDNPNLYLKSNDVILCVLPLFHIYSL 265

Query: 259 SSIVLISIRSGAALLLMEKFEIESLLRLIERHEVTVAAVVPPLVVALVKNPRLADFDLSS 318
           +S++L S+RSGA +LLM KFEI +LL LI+RH VT+AA+VPPLV+AL KNP +  +DLSS
Sbjct: 266 NSVLLNSLRSGATVLLMHKFEIGALLDLIQRHRVTIAALVPPLVIALAKNPTVNSYDLSS 325

Query: 319 IRLVLSGAAPLRKELEEALMERLPQAIFGQGYGMTEAGPVLSMCSAFAKEPLMPTKSGSC 378
           +R VLSGAAPL KEL+++L  RLPQAI GQGYGMTEAGPVLSM   FAKEP+ PTKSGSC
Sbjct: 326 VRFVLSGAAPLGKELQDSLRRRLPQAILGQGYGMTEAGPVLSMSLGFAKEPI-PTKSGSC 385

Query: 379 GRVVRNSELKVIDPQTGDSLTYNQPGEICVRGPQVMKGYLNDPVSTSLTVDVEGWLHTGD 438
           G VVRN+ELKV+  +T  SL YNQPGEIC+RG Q+MK YLNDP +TS T+D EGWLHTGD
Sbjct: 386 GTVVRNAELKVVHLETRLSLGYNQPGEICIRGQQIMKEYLNDPEATSATIDEEGWLHTGD 445

Query: 439 IGYIDDEDEIFIVDRVKEIIKFKGFQVAPAELEALLVTHPSIVDAAVVPQSDDVAGEVPV 498
           IGY+D++DEIFIVDR+KE+IKFKGFQV PAELE+LL+ H SI DAAVVPQ+D+VAGEVPV
Sbjct: 446 IGYVDEDDEIFIVDRLKEVIKFKGFQVPPAELESLLINHHSIADAAVVPQNDEVAGEVPV 505

Query: 499 AFVVPSTQDELTEDAVKEFIAKQVVFYKRLQKVYFVQTIPKSPSGKILRKELKTKL 552
           AFVV S  +++TE+ VKE++AKQVVFYKRL KV+FV +IPKSPSGKILRK+LK KL
Sbjct: 506 AFVVRSNGNDITEEDVKEYVAKQVVFYKRLHKVFFVASIPKSPSGKILRKDLKAKL 560

BLAST of Cla97C02G034290 vs. TAIR 10
Match: AT3G21240.1 (4-coumarate:CoA ligase 2 )

HSP 1 Score: 647.9 bits (1670), Expect = 7.4e-186
Identity = 324/530 (61.13%), Postives = 419/530 (79.06%), Query Frame = 0

Query: 28  FRSKFPDITIPDHLPLHSYCFQNLSEVSHRPCLILGSTGKSYSYSDTHLFSLKAAATFSK 87
           FRS+ PDI IP+HLPLH Y F+N+SE + +PCLI G TG+ Y+Y+D H+ S K AA    
Sbjct: 23  FRSRLPDIYIPNHLPLHDYIFENISEFAAKPCLINGPTGEVYTYADVHVTSRKLAAGLHN 82

Query: 88  LGVKKGDIIMILLHNSPEFVFSFMASSMLGAVATTANPYYTAAEISKQLKASGAKFVVTY 147
           LGVK+ D++MILL NSPE V +F+A+S +GA+ T+ANP++T AEISKQ KAS AK +VT 
Sbjct: 83  LGVKQHDVVMILLPNSPEVVLTFLAASFIGAITTSANPFFTPAEISKQAKASAAKLIVTQ 142

Query: 148 SQCVDKLKE-SGEHLTIVTVDDP--PENCLSFSMVYDADVNDVPSV--EIDTNDAVSLPF 207
           S+ VDK+K    + + IVT D    PENCL FS +  ++   V S+  +I   D V+LPF
Sbjct: 143 SRYVDKIKNLQNDGVLIVTTDSDAIPENCLRFSELTQSEEPRVDSIPEKISPEDVVALPF 202

Query: 208 SSGTTGLPKGVILTHKNMVSSVAQQVDGENPNLYLRRNDVVLCVLPMFHIFSLSSIVLIS 267
           SSGTTGLPKGV+LTHK +V+SVAQQVDGENPNLY  R+DV+LCVLPMFHI++L+SI+L S
Sbjct: 203 SSGTTGLPKGVMLTHKGLVTSVAQQVDGENPNLYFNRDDVILCVLPMFHIYALNSIMLCS 262

Query: 268 IRSGAALLLMEKFEIESLLRLIERHEVTVAAVVPPLVVALVKNPRLADFDLSSIRLVLSG 327
           +R GA +L+M KFEI  LL  I+R +VTVA VVPP+V+A+ K+P    +DLSS+R+V SG
Sbjct: 263 LRVGATILIMPKFEITLLLEQIQRCKVTVAMVVPPIVLAIAKSPETEKYDLSSVRMVKSG 322

Query: 328 AAPLRKELEEALMERLPQAIFGQGYGMTEAGPVLSMCSAFAKEPLMPTKSGSCGRVVRNS 387
           AAPL KELE+A+  + P A  GQGYGMTEAGPVL+M   FAKEP  P KSG+CG VVRN+
Sbjct: 323 AAPLGKELEDAISAKFPNAKLGQGYGMTEAGPVLAMSLGFAKEP-FPVKSGACGTVVRNA 382

Query: 388 ELKVIDPQTGDSLTYNQPGEICVRGPQVMKGYLNDPVSTSLTVDVEGWLHTGDIGYIDDE 447
           E+K++DP TGDSL  N+PGEIC+RG Q+MKGYLNDP++T+ T+D +GWLHTGD+G+IDD+
Sbjct: 383 EMKILDPDTGDSLPRNKPGEICIRGNQIMKGYLNDPLATASTIDKDGWLHTGDVGFIDDD 442

Query: 448 DEIFIVDRVKEIIKFKGFQVAPAELEALLVTHPSIVDAAVVPQSDDVAGEVPVAFVVPST 507
           DE+FIVDR+KE+IK+KGFQVAPAELE+LL+ HP I D AVV   ++ AGEVPVAFVV S 
Sbjct: 443 DELFIVDRLKELIKYKGFQVAPAELESLLIGHPEINDVAVVAMKEEDAGEVPVAFVVRSK 502

Query: 508 QDELTEDAVKEFIAKQVVFYKRLQKVYFVQTIPKSPSGKILRKELKTKLS 553
              ++ED +K+F++KQVVFYKR+ KV+F  +IPK+PSGKILRK+L+ +L+
Sbjct: 503 DSNISEDEIKQFVSKQVVFYKRINKVFFTDSIPKAPSGKILRKDLRARLA 551

BLAST of Cla97C02G034290 vs. TAIR 10
Match: AT1G51680.1 (4-coumarate:CoA ligase 1 )

HSP 1 Score: 646.0 bits (1665), Expect = 2.8e-185
Identity = 326/535 (60.93%), Postives = 417/535 (77.94%), Query Frame = 0

Query: 28  FRSKFPDITIPDHLPLHSYCFQNLSEVSHRPCLILGSTGKSYSYSDTHLFSLKAAATFSK 87
           FRSK PDI IP+HL LH Y FQN+SE + +PCLI G TG  Y+YSD H+ S + AA F K
Sbjct: 25  FRSKLPDIYIPNHLSLHDYIFQNISEFATKPCLINGPTGHVYTYSDVHVISRQIAANFHK 84

Query: 88  LGVKKGDIIMILLHNSPEFVFSFMASSMLGAVATTANPYYTAAEISKQLKASGAKFVVTY 147
           LGV + D++M+LL N PEFV SF+A+S  GA AT ANP++T AEI+KQ KAS  K ++T 
Sbjct: 85  LGVNQNDVVMLLLPNCPEFVLSFLAASFRGATATAANPFFTPAEIAKQAKASNTKLIITE 144

Query: 148 SQCVDKLK--ESGEHLTIVTVDDP-----PENCLSFSMVYDADVND---VPSVEIDTNDA 207
           ++ VDK+K  ++ + + IV +DD      PE CL F+ +  +       + SVEI  +D 
Sbjct: 145 ARYVDKIKPLQNDDGVVIVCIDDNESVPIPEGCLRFTELTQSTTEASEVIDSVEISPDDV 204

Query: 208 VSLPFSSGTTGLPKGVILTHKNMVSSVAQQVDGENPNLYLRRNDVVLCVLPMFHIFSLSS 267
           V+LP+SSGTTGLPKGV+LTHK +V+SVAQQVDGENPNLY   +DV+LCVLPMFHI++L+S
Sbjct: 205 VALPYSSGTTGLPKGVMLTHKGLVTSVAQQVDGENPNLYFHSDDVILCVLPMFHIYALNS 264

Query: 268 IVLISIRSGAALLLMEKFEIESLLRLIERHEVTVAAVVPPLVVALVKNPRLADFDLSSIR 327
           I+L  +R GAA+L+M KFEI  LL LI+R +VTVA +VPP+V+A+ K+     +DLSSIR
Sbjct: 265 IMLCGLRVGAAILIMPKFEINLLLELIQRCKVTVAPMVPPIVLAIAKSSETEKYDLSSIR 324

Query: 328 LVLSGAAPLRKELEEALMERLPQAIFGQGYGMTEAGPVLSMCSAFAKEPLMPTKSGSCGR 387
           +V SGAAPL KELE+A+  + P A  GQGYGMTEAGPVL+M   FAKEP  P KSG+CG 
Sbjct: 325 VVKSGAAPLGKELEDAVNAKFPNAKLGQGYGMTEAGPVLAMSLGFAKEP-FPVKSGACGT 384

Query: 388 VVRNSELKVIDPQTGDSLTYNQPGEICVRGPQVMKGYLNDPVSTSLTVDVEGWLHTGDIG 447
           VVRN+E+K++DP TGDSL+ NQPGEIC+RG Q+MKGYLN+P +T+ T+D +GWLHTGDIG
Sbjct: 385 VVRNAEMKIVDPDTGDSLSRNQPGEICIRGHQIMKGYLNNPAATAETIDKDGWLHTGDIG 444

Query: 448 YIDDEDEIFIVDRVKEIIKFKGFQVAPAELEALLVTHPSIVDAAVVPQSDDVAGEVPVAF 507
            IDD+DE+FIVDR+KE+IK+KGFQVAPAELEALL+ HP I D AVV   ++ AGEVPVAF
Sbjct: 445 LIDDDDELFIVDRLKELIKYKGFQVAPAELEALLIGHPDITDVAVVAMKEEAAGEVPVAF 504

Query: 508 VVPSTQDELTEDAVKEFIAKQVVFYKRLQKVYFVQTIPKSPSGKILRKELKTKLS 553
           VV S   EL+ED VK+F++KQVVFYKR+ KV+F ++IPK+PSGKILRK+L+ KL+
Sbjct: 505 VVKSKDSELSEDDVKQFVSKQVVFYKRINKVFFTESIPKAPSGKILRKDLRAKLA 558

BLAST of Cla97C02G034290 vs. TAIR 10
Match: AT1G65060.2 (4-coumarate:CoA ligase 3 )

HSP 1 Score: 610.5 bits (1573), Expect = 1.3e-174
Identity = 302/449 (67.26%), Postives = 375/449 (83.52%), Query Frame = 0

Query: 19  PPSTATTHTFRSKFPDITIPDHLPLHSYCFQNLSEVSHRPCLILGSTGKSYSYSDTHLFS 78
           P S  T   FRSK PDI IP+HLPLH+YCF+ LS VS +PCLI+GSTGKSY+Y +THL  
Sbjct: 26  PHSPPTPRIFRSKLPDIDIPNHLPLHTYCFEKLSSVSDKPCLIVGSTGKSYTYGETHLIC 85

Query: 79  LKAAATFSKLGVKKGDIIMILLHNSPEFVFSFMASSMLGAVATTANPYYTAAEISKQLKA 138
            + A+   KLG++KGD+IMILL NS EFVFSFM +SM+GAV+TTANP+YT+ E+ KQLK+
Sbjct: 86  RRVASGLYKLGIRKGDVIMILLQNSAEFVFSFMGASMIGAVSTTANPFYTSQELYKQLKS 145

Query: 139 SGAKFVVTYSQCVDKLKESGEHLTIVTVDDP-PENCLSFS-MVYDADVNDV-PSVEIDTN 198
           SGAK ++T+SQ VDKLK  GE+LT++T D+P PENCL FS ++ D + N    +V+I  +
Sbjct: 146 SGAKLIITHSQYVDKLKNLGENLTLITTDEPTPENCLPFSTLITDDETNPFQETVDIGGD 205

Query: 199 DAVSLPFSSGTTGLPKGVILTHKNMVSSVAQQVDGENPNLYLRRNDVVLCVLPMFHIFSL 258
           DA +LPFSSGTTGLPKGV+LTHK++++SVAQQVDG+NPNLYL+ NDV+LCVLP+FHI+SL
Sbjct: 206 DAAALPFSSGTTGLPKGVVLTHKSLITSVAQQVDGDNPNLYLKSNDVILCVLPLFHIYSL 265

Query: 259 SSIVLISIRSGAALLLMEKFEIESLLRLIERHEVTVAAVVPPLVVALVKNPRLADFDLSS 318
           +S++L S+RSGA +LLM KFEI +LL LI+RH VT+AA+VPPLV+AL KNP +  +DLSS
Sbjct: 266 NSVLLNSLRSGATVLLMHKFEIGALLDLIQRHRVTIAALVPPLVIALAKNPTVNSYDLSS 325

Query: 319 IRLVLSGAAPLRKELEEALMERLPQAIFGQGYGMTEAGPVLSMCSAFAKEPLMPTKSGSC 378
           +R VLSGAAPL KEL+++L  RLPQAI GQGYGMTEAGPVLSM   FAKEP+ PTKSGSC
Sbjct: 326 VRFVLSGAAPLGKELQDSLRRRLPQAILGQGYGMTEAGPVLSMSLGFAKEPI-PTKSGSC 385

Query: 379 GRVVRNSELKVIDPQTGDSLTYNQPGEICVRGPQVMKGYLNDPVSTSLTVDVEGWLHTGD 438
           G VVRN+ELKV+  +T  SL YNQPGEIC+RG Q+MK YLNDP +TS T+D EGWLHTGD
Sbjct: 386 GTVVRNAELKVVHLETRLSLGYNQPGEICIRGQQIMKEYLNDPEATSATIDEEGWLHTGD 445

Query: 439 IGYIDDEDEIFIVDRVKEIIKFKGFQVAP 465
           IGY+D++DEIFIVDR+KE+IKFKGFQ +P
Sbjct: 446 IGYVDEDDEIFIVDRLKEVIKFKGFQASP 473

BLAST of Cla97C02G034290 vs. TAIR 10
Match: AT1G51680.3 (4-coumarate:CoA ligase 1 )

HSP 1 Score: 599.0 bits (1543), Expect = 4.0e-171
Identity = 304/502 (60.56%), Postives = 387/502 (77.09%), Query Frame = 0

Query: 28  FRSKFPDITIPDHLPLHSYCFQNLSEVSHRPCLILGSTGKSYSYSDTHLFSLKAAATFSK 87
           FRSK PDI IP+HL LH Y FQN+SE + +PCLI G TG  Y+YSD H+ S + AA F K
Sbjct: 25  FRSKLPDIYIPNHLSLHDYIFQNISEFATKPCLINGPTGHVYTYSDVHVISRQIAANFHK 84

Query: 88  LGVKKGDIIMILLHNSPEFVFSFMASSMLGAVATTANPYYTAAEISKQLKASGAKFVVTY 147
           LGV + D++M+LL N PEFV SF+A+S  GA AT ANP++T AEI+KQ KAS  K ++T 
Sbjct: 85  LGVNQNDVVMLLLPNCPEFVLSFLAASFRGATATAANPFFTPAEIAKQAKASNTKLIITE 144

Query: 148 SQCVDKLK--ESGEHLTIVTVDDP-----PENCLSFSMVYDADVND---VPSVEIDTNDA 207
           ++ VDK+K  ++ + + IV +DD      PE CL F+ +  +       + SVEI  +D 
Sbjct: 145 ARYVDKIKPLQNDDGVVIVCIDDNESVPIPEGCLRFTELTQSTTEASEVIDSVEISPDDV 204

Query: 208 VSLPFSSGTTGLPKGVILTHKNMVSSVAQQVDGENPNLYLRRNDVVLCVLPMFHIFSLSS 267
           V+LP+SSGTTGLPKGV+LTHK +V+SVAQQVDGENPNLY   +DV+LCVLPMFHI++L+S
Sbjct: 205 VALPYSSGTTGLPKGVMLTHKGLVTSVAQQVDGENPNLYFHSDDVILCVLPMFHIYALNS 264

Query: 268 IVLISIRSGAALLLMEKFEIESLLRLIERHEVTVAAVVPPLVVALVKNPRLADFDLSSIR 327
           I+L  +R GAA+L+M KFEI  LL LI+R +VTVA +VPP+V+A+ K+     +DLSSIR
Sbjct: 265 IMLCGLRVGAAILIMPKFEINLLLELIQRCKVTVAPMVPPIVLAIAKSSETEKYDLSSIR 324

Query: 328 LVLSGAAPLRKELEEALMERLPQAIFGQGYGMTEAGPVLSMCSAFAKEPLMPTKSGSCGR 387
           +V SGAAPL KELE+A+  + P A  GQGYGMTEAGPVL+M   FAKEP  P KSG+CG 
Sbjct: 325 VVKSGAAPLGKELEDAVNAKFPNAKLGQGYGMTEAGPVLAMSLGFAKEP-FPVKSGACGT 384

Query: 388 VVRNSELKVIDPQTGDSLTYNQPGEICVRGPQVMKGYLNDPVSTSLTVDVEGWLHTGDIG 447
           VVRN+E+K++DP TGDSL+ NQPGEIC+RG Q+MKGYLN+P +T+ T+D +GWLHTGDIG
Sbjct: 385 VVRNAEMKIVDPDTGDSLSRNQPGEICIRGHQIMKGYLNNPAATAETIDKDGWLHTGDIG 444

Query: 448 YIDDEDEIFIVDRVKEIIKFKGFQVAPAELEALLVTHPSIVDAAVVPQSDDVAGEVPVAF 507
            IDD+DE+FIVDR+KE+IK+KGFQVAPAELEALL+ HP I D AVV   ++ AGEVPVAF
Sbjct: 445 LIDDDDELFIVDRLKELIKYKGFQVAPAELEALLIGHPDITDVAVVAMKEEAAGEVPVAF 504

Query: 508 VVPSTQDELTEDAVKEFIAKQV 520
           VV S   EL+ED VK+F++KQV
Sbjct: 505 VVKSKDSELSEDDVKQFVSKQV 525

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
XP_038901546.11.2e-28392.044-coumarate--CoA ligase 3 isoform X1 [Benincasa hispida][more]
XP_008462779.11.5e-27689.25PREDICTED: 4-coumarate--CoA ligase 2 [Cucumis melo] >KAA0042418.1 4-coumarate--C... [more]
XP_038901547.12.0e-27389.874-coumarate--CoA ligase 3 isoform X2 [Benincasa hispida][more]
XP_004151908.13.7e-27287.614-coumarate--CoA ligase 2 [Cucumis sativus] >KAE8652343.1 hypothetical protein C... [more]
XP_022947959.16.4e-24879.324-coumarate--CoA ligase 3 [Cucurbita moschata][more]
Match NameE-valueIdentityDescription
P316873.0e-21670.344-coumarate--CoA ligase 2 OS=Glycine max OX=3847 PE=2 SV=2[more]
Q9S7777.2e-21068.474-coumarate--CoA ligase 3 OS=Arabidopsis thaliana OX=3702 GN=4CL3 PE=1 SV=1[more]
Q429823.8e-19562.004-coumarate--CoA ligase 2 OS=Oryza sativa subsp. japonica OX=39947 GN=4CL2 PE=1 ... [more]
A0A2H5AIY44.7e-19362.434-coumarate-CoA ligase 2 OS=Narcissus pseudonarcissus OX=39639 GN=4CL2 PE=2 SV=1[more]
I3PB374.9e-19061.294-coumarate:CoA ligase 1 OS=Petunia hybrida OX=4102 GN=4CL1 PE=1 SV=1[more]
Match NameE-valueIdentityDescription
A0A5A7THZ87.1e-27789.254-coumarate--CoA ligase 2 OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffo... [more]
A0A1S3CJB37.1e-27789.254-coumarate--CoA ligase 2 OS=Cucumis melo OX=3656 GN=LOC103501067 PE=4 SV=1[more]
A0A6J1G8F93.1e-24879.324-coumarate--CoA ligase 3 OS=Cucurbita moschata OX=3662 GN=LOC111451685 PE=4 SV=... [more]
A0A0A0LQV11.0e-23888.30AMP-binding domain-containing protein OS=Cucumis sativus OX=3659 GN=Csa_2G433350... [more]
A0A4Y1R2Z53.6e-22071.484-coumarate:CoA ligase 3 OS=Prunus dulcis OX=3755 GN=ALMOND_2B015040 PE=4 SV=1[more]
Match NameE-valueIdentityDescription
AT1G65060.15.1e-21168.474-coumarate:CoA ligase 3 [more]
AT3G21240.17.4e-18661.134-coumarate:CoA ligase 2 [more]
AT1G51680.12.8e-18560.934-coumarate:CoA ligase 1 [more]
AT1G65060.21.3e-17467.264-coumarate:CoA ligase 3 [more]
AT1G51680.34.0e-17160.564-coumarate:CoA ligase 1 [more]
InterPro
Analysis Name: InterPro Annotations of Watermelon (97103) v2.5
Date Performed: 2022-01-31
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR000873AMP-dependent synthetase/ligasePFAMPF00501AMP-bindingcoord: 56..457
e-value: 1.6E-104
score: 349.9
IPR042099ANL, N-terminal domainGENE3D3.40.50.12780coord: 21..451
e-value: 3.0E-153
score: 512.5
IPR025110AMP-binding enzyme, C-terminal domainPFAMPF13193AMP-binding_Ccoord: 466..541
e-value: 2.8E-17
score: 63.4
NoneNo IPR availableGENE3D3.30.300.30coord: 452..553
e-value: 7.8E-30
score: 105.5
NoneNo IPR availableMOBIDB_LITEmobidb-litedisorder_predictioncoord: 1..25
NoneNo IPR availablePANTHERPTHR24096:SF1694-COUMARATE--COA LIGASE 3coord: 27..550
NoneNo IPR availablePANTHERPTHR24096LONG-CHAIN-FATTY-ACID--COA LIGASEcoord: 27..550
NoneNo IPR availableCDDcd059044CLcoord: 37..547
e-value: 0.0
score: 690.513
NoneNo IPR availableSUPERFAMILY56801Acetyl-CoA synthetase-likecoord: 26..550
IPR020845AMP-binding, conserved sitePROSITEPS00455AMP_BINDINGcoord: 200..211

Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
Cla97C02G034290.1Cla97C02G034290.1mRNA


GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
biological_process GO:0009698 phenylpropanoid metabolic process
biological_process GO:0010584 pollen exine formation
cellular_component GO:0005737 cytoplasm
molecular_function GO:0016207 4-coumarate-CoA ligase activity
molecular_function GO:0106290 trans-cinnamate-CoA ligase activity