Cla97C02G026900 (gene) Watermelon (97103) v2.5

Overview
NameCla97C02G026900
Typegene
OrganismCitrullus lanatus subsp. vulgaris cv. 97103 (Watermelon (97103) v2.5)
DescriptionRING-type E3 ubiquitin transferase
LocationCla97Chr02: 607371 .. 609075 (+)
RNA-Seq ExpressionCla97C02G026900
SyntenyCla97C02G026900
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: exonfive_prime_UTRCDSpolypeptidethree_prime_UTR
Hold the cursor over a type above to highlight its positions in the sequence below.
AAAGAAGTGAATAAAGAGGGAAGAGAAAAAGTAGGATGACATATGGGAATTGGGAATTTGGAGCGTACCTTTGACTCTCTATCTTTAAACCTTTCTTCCTCTGCTTTCATCAATCTTTTTCTTCTTCTTCTTCCCATTAACAATTTACAGTTCATCGCCATGGTCAGGCCGCACAATCTCTTCATCACTGTTCCCACTCACTTCTGTTGCCCAATATCTCTCGACCTCATGCAATCTCCCGTCAGTTTGACCACCGGTGTCACCTACGACCGCTCCAGCATCCAGCGCTGGCTCGACTCCGGCCATAATACTTGCCCCGCCACCATGCAGATCCTCCACAACTACGACTTCGTTCCCAATTCTAATCTCAAGCGACTCATTCAGATTTGGTCCGATTCCCTCCAATTCCCCCTCTCTACACTCTCCCAATCGGATCATATTCTTACCGCTGTCGATGATTTGCGCACAAACGGAAATGGCTCTGTTTCAGTTTCTGTTTCTGTCTCTGCTTCTCTATCCAAGCTACTCTGTTTCGGTGCACGATTGGAGCAGAACGTCAAGTTTCTCGGGAGGATTCACGGCTTTGTACCGGTGCTTCTCGATGTTCTTCGTTGTAGAGATGTTGATTGTTCGGAACTGGTGGTTAGGGTTTTGGATTTGGTGAGATGCGAAATTGAGGATCGGGAGGAGTTTATGAACATGATGTTGAAAAGCGATCGCGATTGTTTGAGATCTCTGGTTCTCGTTCTACAGCGAGGAAGTTCGGAATCGCGAATTGGAACAGCGAGGCTTCTCGAGTGCGTCGCCATTAACGCTGAATCAAAGAACTTAATTGCCGAAAACGAAGCTATTCTACACGAATTGATTGGAGTAATCGGAATAGACGAAGATCCGAAACTAATAGAATCGGTGCTCTCGTGCTTAATCGCAATATCAATGCCGAAACGAGTGAAAATCAAACTCGTTCGATTAGGAGTAATAAAAGCCCTAACCAGACTGCTGAAGCACCGGAACGCAAGCGTGGGAGTAACGGAGAAGGTTCTGAGGCTATTAGCGGCGGCGGCGGCGGTAGAGGAAGGGCGGTCGGAGATGTTGGAGAACGGAGGAGAATGCGTGGGGGGCATGGTGAAGAAAGTGATGAAGGTGTCGAGTGCGGCGACGGAGCAGGCGGTGACGGCGCTGTGGTGCATATGCTATTTGTTCCGGGAAGAGAGGGCGGCGGAGGCGGTGGCGGAGGCGAAAGGGGTTGAGAAGATACTGTTGTTGATGCAGAGCCATTGTCCGGCGACGGTCAGGCAAATGGCCAAAGATCTTCTAAAGATTTTGAAGGTTAATTCCATCTCCTCTTGCTTATCAATTTATGATACTAACAATTCTTACATTATGCCCTTTTGAATTTAAATCTAAATTATAATCATTAATTAATACTTAACATTACACTGTTAATTAACTAATTAATCCAACACTTTGGGTTAAATTGTTCATATAACACATGTAGAGAAGCTGAAGAAAGTGATGTGATTCAAAGGGTTGATTTGTAAATTTGAGTAACATTTTTTTCAAAGTGAATACAAAGTTGGGATTCATTTTTATTACAAGTTTGTAATGTGTGTGGTCAACGCCATTCCCAATCTTATACTTTACTCCAAAAGGTTGAAAGATTCAAATTATTTACTTAATCTACTCATACAAAGTAAGAAAGTGT

mRNA sequence

AAAGAAGTGAATAAAGAGGGAAGAGAAAAAGTAGGATGACATATGGGAATTGGGAATTTGGAGCGTACCTTTGACTCTCTATCTTTAAACCTTTCTTCCTCTGCTTTCATCAATCTTTTTCTTCTTCTTCTTCCCATTAACAATTTACAGTTCATCGCCATGGTCAGGCCGCACAATCTCTTCATCACTGTTCCCACTCACTTCTGTTGCCCAATATCTCTCGACCTCATGCAATCTCCCGTCAGTTTGACCACCGGTGTCACCTACGACCGCTCCAGCATCCAGCGCTGGCTCGACTCCGGCCATAATACTTGCCCCGCCACCATGCAGATCCTCCACAACTACGACTTCGTTCCCAATTCTAATCTCAAGCGACTCATTCAGATTTGGTCCGATTCCCTCCAATTCCCCCTCTCTACACTCTCCCAATCGGATCATATTCTTACCGCTGTCGATGATTTGCGCACAAACGGAAATGGCTCTGTTTCAGTTTCTGTTTCTGTCTCTGCTTCTCTATCCAAGCTACTCTGTTTCGGTGCACGATTGGAGCAGAACGTCAAGTTTCTCGGGAGGATTCACGGCTTTGTACCGGTGCTTCTCGATGTTCTTCGTTGTAGAGATGTTGATTGTTCGGAACTGGTGGTTAGGGTTTTGGATTTGGTGAGATGCGAAATTGAGGATCGGGAGGAGTTTATGAACATGATGTTGAAAAGCGATCGCGATTGTTTGAGATCTCTGGTTCTCGTTCTACAGCGAGGAAGTTCGGAATCGCGAATTGGAACAGCGAGGCTTCTCGAGTGCGTCGCCATTAACGCTGAATCAAAGAACTTAATTGCCGAAAACGAAGCTATTCTACACGAATTGATTGGAGTAATCGGAATAGACGAAGATCCGAAACTAATAGAATCGGTGCTCTCGTGCTTAATCGCAATATCAATGCCGAAACGAGTGAAAATCAAACTCGTTCGATTAGGAGTAATAAAAGCCCTAACCAGACTGCTGAAGCACCGGAACGCAAGCGTGGGAGTAACGGAGAAGGTTCTGAGGCTATTAGCGGCGGCGGCGGCGGTAGAGGAAGGGCGGTCGGAGATGTTGGAGAACGGAGGAGAATGCGTGGGGGGCATGGTGAAGAAAGTGATGAAGGTGTCGAGTGCGGCGACGGAGCAGGCGGTGACGGCGCTGTGGTGCATATGCTATTTGTTCCGGGAAGAGAGGGCGGCGGAGGCGGTGGCGGAGGCGAAAGGGGTTGAGAAGATACTGTTGTTGATGCAGAGCCATTGTCCGGCGACGGTCAGGCAAATGGCCAAAGATCTTCTAAAGATTTTGAAGGTTAATTCCATCTCCTCTTGCTTATCAATTTATGATACTAACAATTCTTACATTATGCCCTTTTGAATTTAAATCTAAATTATAATCATTAATTAATACTTAACATTACACTGTTAATTAACTAATTAATCCAACACTTTGGGTTAAATTGTTCATATAACACATGTAGAGAAGCTGAAGAAAGTGATGTGATTCAAAGGGTTGATTTGTAAATTTGAGTAACATTTTTTTCAAAGTGAATACAAAGTTGGGATTCATTTTTATTACAAGTTTGTAATGTGTGTGGTCAACGCCATTCCCAATCTTATACTTTACTCCAAAAGGTTGAAAGATTCAAATTATTTACTTAATCTACTCATACAAAGTAAGAAAGTGT

Coding sequence (CDS)

ATGGGAATTGGGAATTTGGAGCGTACCTTTGACTCTCTATCTTTAAACCTTTCTTCCTCTGCTTTCATCAATCTTTTTCTTCTTCTTCTTCCCATTAACAATTTACAGTTCATCGCCATGGTCAGGCCGCACAATCTCTTCATCACTGTTCCCACTCACTTCTGTTGCCCAATATCTCTCGACCTCATGCAATCTCCCGTCAGTTTGACCACCGGTGTCACCTACGACCGCTCCAGCATCCAGCGCTGGCTCGACTCCGGCCATAATACTTGCCCCGCCACCATGCAGATCCTCCACAACTACGACTTCGTTCCCAATTCTAATCTCAAGCGACTCATTCAGATTTGGTCCGATTCCCTCCAATTCCCCCTCTCTACACTCTCCCAATCGGATCATATTCTTACCGCTGTCGATGATTTGCGCACAAACGGAAATGGCTCTGTTTCAGTTTCTGTTTCTGTCTCTGCTTCTCTATCCAAGCTACTCTGTTTCGGTGCACGATTGGAGCAGAACGTCAAGTTTCTCGGGAGGATTCACGGCTTTGTACCGGTGCTTCTCGATGTTCTTCGTTGTAGAGATGTTGATTGTTCGGAACTGGTGGTTAGGGTTTTGGATTTGGTGAGATGCGAAATTGAGGATCGGGAGGAGTTTATGAACATGATGTTGAAAAGCGATCGCGATTGTTTGAGATCTCTGGTTCTCGTTCTACAGCGAGGAAGTTCGGAATCGCGAATTGGAACAGCGAGGCTTCTCGAGTGCGTCGCCATTAACGCTGAATCAAAGAACTTAATTGCCGAAAACGAAGCTATTCTACACGAATTGATTGGAGTAATCGGAATAGACGAAGATCCGAAACTAATAGAATCGGTGCTCTCGTGCTTAATCGCAATATCAATGCCGAAACGAGTGAAAATCAAACTCGTTCGATTAGGAGTAATAAAAGCCCTAACCAGACTGCTGAAGCACCGGAACGCAAGCGTGGGAGTAACGGAGAAGGTTCTGAGGCTATTAGCGGCGGCGGCGGCGGTAGAGGAAGGGCGGTCGGAGATGTTGGAGAACGGAGGAGAATGCGTGGGGGGCATGGTGAAGAAAGTGATGAAGGTGTCGAGTGCGGCGACGGAGCAGGCGGTGACGGCGCTGTGGTGCATATGCTATTTGTTCCGGGAAGAGAGGGCGGCGGAGGCGGTGGCGGAGGCGAAAGGGGTTGAGAAGATACTGTTGTTGATGCAGAGCCATTGTCCGGCGACGGTCAGGCAAATGGCCAAAGATCTTCTAAAGATTTTGAAGGTTAATTCCATCTCCTCTTGCTTATCAATTTATGATACTAACAATTCTTACATTATGCCCTTTTGA

Protein sequence

MGIGNLERTFDSLSLNLSSSAFINLFLLLLPINNLQFIAMVRPHNLFITVPTHFCCPISLDLMQSPVSLTTGVTYDRSSIQRWLDSGHNTCPATMQILHNYDFVPNSNLKRLIQIWSDSLQFPLSTLSQSDHILTAVDDLRTNGNGSVSVSVSVSASLSKLLCFGARLEQNVKFLGRIHGFVPVLLDVLRCRDVDCSELVVRVLDLVRCEIEDREEFMNMMLKSDRDCLRSLVLVLQRGSSESRIGTARLLECVAINAESKNLIAENEAILHELIGVIGIDEDPKLIESVLSCLIAISMPKRVKIKLVRLGVIKALTRLLKHRNASVGVTEKVLRLLAAAAAVEEGRSEMLENGGECVGGMVKKVMKVSSAATEQAVTALWCICYLFREERAAEAVAEAKGVEKILLLMQSHCPATVRQMAKDLLKILKVNSISSCLSIYDTNNSYIMPF
Homology
BLAST of Cla97C02G026900 vs. NCBI nr
Match: XP_038901738.1 (U-box domain-containing protein 27-like [Benincasa hispida])

HSP 1 Score: 706.1 bits (1821), Expect = 2.0e-199
Identity = 374/410 (91.22%), Postives = 385/410 (93.90%), Query Frame = 0

Query: 40  MVRPHNLFITVPTHFCCPISLDLMQSPVSLTTGVTYDRSSIQRWLDSGHNTCPATMQILH 99
           MVRPH+LFI VPTHF CPISLDLMQSPV+LTTG+TYDRSSIQ WLDSGHNTCPATMQ+LH
Sbjct: 1   MVRPHDLFINVPTHFLCPISLDLMQSPVTLTTGITYDRSSIQHWLDSGHNTCPATMQVLH 60

Query: 100 NYDFVPNSNLKRLIQIWSDSLQFPLSTLSQSDHILTAVDDLRTNGNGSVSVSVSVSASLS 159
           NYD VPNS+LKRLIQIWSDS    L  LSQSDHILT VDDL TN NG  SVS+SVSASLS
Sbjct: 61  NYDLVPNSSLKRLIQIWSDS----LLDLSQSDHILTLVDDLHTNRNG--SVSLSVSASLS 120

Query: 160 KLLCFGARLEQNVKFLGRIHGFVPVLLDVLRCRDVDCSELVVRVLDLVRCEIEDREEFMN 219
           KLLCFGARLEQNVKFLGRIHGFVPVLLDVLRCRDVDCSELVVRVLDLVRCEI DREEFMN
Sbjct: 121 KLLCFGARLEQNVKFLGRIHGFVPVLLDVLRCRDVDCSELVVRVLDLVRCEIVDREEFMN 180

Query: 220 MMLKSDRDCLRSLVLVLQRGSSESRIGTARLLECVAINAESKNLIAENEAILHELIGVIG 279
           MMLKSDRDCLRSLVLVLQRGSSESRIGTARLLECVAINAESKNLIAENE ILHELIGVIG
Sbjct: 181 MMLKSDRDCLRSLVLVLQRGSSESRIGTARLLECVAINAESKNLIAENEGILHELIGVIG 240

Query: 280 IDEDPKLIESVLSCLIAISMPKRVKIKLVRLGVIKALTRLLKHRNASVGVTEKVLRLLAA 339
           IDEDPK+IESVLSCLIAISMPKRVKIKLVRLGVIKALTRLLKHRN SV +TEKVLRLLAA
Sbjct: 241 IDEDPKVIESVLSCLIAISMPKRVKIKLVRLGVIKALTRLLKHRNVSVEITEKVLRLLAA 300

Query: 340 AAAVEEGRSEMLENGGECVGGMVKKVMKVSSAATEQAVTALWCICYLFREERAAEAVAEA 399
           AAAVEEGRSEMLENGGECVG MV+KVMKVSSAATE AVTALWCICYLFREE+AAEAVAEA
Sbjct: 301 AAAVEEGRSEMLENGGECVGRMVRKVMKVSSAATEHAVTALWCICYLFREEKAAEAVAEA 360

Query: 400 KGVEKILLLMQSHCPATVRQMAKDLLKILKVNSISSCLSIYDTNNSYIMP 450
           KGVE ILLLMQSHCPATVRQMAKDLL+ILKVNSIS CLSIYDT N YIMP
Sbjct: 361 KGVENILLLMQSHCPATVRQMAKDLLEILKVNSISYCLSIYDTANFYIMP 404

BLAST of Cla97C02G026900 vs. NCBI nr
Match: XP_008461027.1 (PREDICTED: LOW QUALITY PROTEIN: U-box domain-containing protein 27 [Cucumis melo])

HSP 1 Score: 647.9 bits (1670), Expect = 6.5e-182
Identity = 342/390 (87.69%), Postives = 356/390 (91.28%), Query Frame = 0

Query: 40  MVRPHNLFITVPTHFCCPISLDLMQSPVSLTTGVTYDRSSIQRWLDSGHNTCPATMQILH 99
           MV+PHNLF+T+PTHF CPISLDLMQSPVSL TGVTYDRSSIQRWLDSGHNTCPATMQ+L 
Sbjct: 1   MVKPHNLFLTIPTHFICPISLDLMQSPVSLITGVTYDRSSIQRWLDSGHNTCPATMQVLP 60

Query: 100 NYDFVPNSNLKRLIQIWSDSLQFPLSTLSQSDHILTAVDDLRTNGNGSVSVSVSVSASLS 159
           NY FVPNSNLKRLIQIWSDSL+F        DHILT VD LRTNGN SV         LS
Sbjct: 61  NYGFVPNSNLKRLIQIWSDSLEF--------DHILTVVDGLRTNGNASV--------FLS 120

Query: 160 KLLCFGARLEQNVKFLGRIHGFVPVLLDVLRCRDVDCSELVVRVLDLVRCEIEDREEFMN 219
           +LLCFG+RLE+NVKFLGRIHGFVPVLLDVLRCRDVDCSELVVRVLDLVRCEIEDR EFMN
Sbjct: 121 ELLCFGSRLEKNVKFLGRIHGFVPVLLDVLRCRDVDCSELVVRVLDLVRCEIEDRVEFMN 180

Query: 220 MMLKSDRDCLRSLVLVLQRGSSESRIGTARLLECVAINAESKNLIAENEAILHELIGVIG 279
           +MLKSDRDCL+SLVLVLQRGSSESRIGT RLLEC+AINAESKNLIAENE ILHELI VIG
Sbjct: 181 LMLKSDRDCLKSLVLVLQRGSSESRIGTVRLLECIAINAESKNLIAENEGILHELIXVIG 240

Query: 280 IDEDPKLIESVLSCLIAISMPKRVKIKLVRLGVIKALTRLLKHRNASVGVTEKVLRLLAA 339
           IDEDPKLIESVLSCLIAISMPKRVKIKLVRLGVIKALTRLLKHRNASVGVTEKVLRLLAA
Sbjct: 241 IDEDPKLIESVLSCLIAISMPKRVKIKLVRLGVIKALTRLLKHRNASVGVTEKVLRLLAA 300

Query: 340 AAAVEEGRSEMLENGGECVGGMVKKVMKVSSAATEQAVTALWCICYLFREERAAEAVAEA 399
           AAAVEEGRSEM+ NGGECVG MV+KVMKVSSAATEQAVTALWCICYLFREE+AA A AEA
Sbjct: 301 AAAVEEGRSEMMVNGGECVGRMVRKVMKVSSAATEQAVTALWCICYLFREEKAAVAAAEA 360

Query: 400 KGVEKILLLMQSHCPATVRQMAKDLLKILK 430
           KGVEKILLLMQSHCPATVR MAKDLLKI K
Sbjct: 361 KGVEKILLLMQSHCPATVRVMAKDLLKIFK 374

BLAST of Cla97C02G026900 vs. NCBI nr
Match: XP_004147378.1 (U-box domain-containing protein 27 [Cucumis sativus] >KGN62142.1 hypothetical protein Csa_006074 [Cucumis sativus])

HSP 1 Score: 644.0 bits (1660), Expect = 9.3e-181
Identity = 341/390 (87.44%), Postives = 354/390 (90.77%), Query Frame = 0

Query: 40  MVRPHNLFITVPTHFCCPISLDLMQSPVSLTTGVTYDRSSIQRWLDSGHNTCPATMQILH 99
           MV+PHNLFIT+PTHF CPISLDLMQSPVSL TGVTYDRSSIQRWLDSGHNTCPATMQ+L 
Sbjct: 1   MVKPHNLFITIPTHFLCPISLDLMQSPVSLITGVTYDRSSIQRWLDSGHNTCPATMQVLT 60

Query: 100 NYDFVPNSNLKRLIQIWSDSLQFPLSTLSQSDHILTAVDDLRTNGNGSVSVSVSVSASLS 159
           NYDFVPNSNLKRLIQIWSDSL+         DHILT VD LRTNGN SV        SLS
Sbjct: 61  NYDFVPNSNLKRLIQIWSDSLEL--------DHILTVVDGLRTNGNASV--------SLS 120

Query: 160 KLLCFGARLEQNVKFLGRIHGFVPVLLDVLRCRDVDCSELVVRVLDLVRCEIEDREEFMN 219
           +LLCFG+RLE+NVKFLGRI GFVPVLLDVLRCRDVDCSELVVRVLDLVRCEIEDR EFMN
Sbjct: 121 ELLCFGSRLEKNVKFLGRIRGFVPVLLDVLRCRDVDCSELVVRVLDLVRCEIEDRVEFMN 180

Query: 220 MMLKSDRDCLRSLVLVLQRGSSESRIGTARLLECVAINAESKNLIAENEAILHELIGVIG 279
           +MLKSDRDCLRSLVLVLQRGSSESRIGT RLLEC+AINAESKNLIAENE ILHELI VIG
Sbjct: 181 LMLKSDRDCLRSLVLVLQRGSSESRIGTVRLLECIAINAESKNLIAENEGILHELIEVIG 240

Query: 280 IDEDPKLIESVLSCLIAISMPKRVKIKLVRLGVIKALTRLLKHRNASVGVTEKVLRLLAA 339
           IDEDPKLIESVLSCLI ISMPKRVKIKLVRLGVIKALTRLLKH+NASVGVTEKVLRLLAA
Sbjct: 241 IDEDPKLIESVLSCLIPISMPKRVKIKLVRLGVIKALTRLLKHQNASVGVTEKVLRLLAA 300

Query: 340 AAAVEEGRSEMLENGGECVGGMVKKVMKVSSAATEQAVTALWCICYLFREERAAEAVAEA 399
           AAAVEEGR EM+ENGGECVG MV+KVMKVSSAATEQAVTALWCICYL+REERAA A AEA
Sbjct: 301 AAAVEEGRWEMMENGGECVGRMVRKVMKVSSAATEQAVTALWCICYLYREERAAVAAAEA 360

Query: 400 KGVEKILLLMQSHCPATVRQMAKDLLKILK 430
           KGVEKILLLMQSHCPATVR MAKDLLK  K
Sbjct: 361 KGVEKILLLMQSHCPATVRAMAKDLLKTFK 374

BLAST of Cla97C02G026900 vs. NCBI nr
Match: XP_023534066.1 (U-box domain-containing protein 27-like [Cucurbita pepo subsp. pepo])

HSP 1 Score: 587.4 bits (1513), Expect = 1.0e-163
Identity = 323/417 (77.46%), Postives = 353/417 (84.65%), Query Frame = 0

Query: 40  MVRPHNLFITVPTHFCCPISLDLMQSPVSLTTGVTYDRSSIQRWLDSGHNTCPATMQILH 99
           MV+ +NLFITVPTHF CPISLDLM+SPVSL TGVTYDRSSIQRWLDSG+NTCPATMQ+L 
Sbjct: 1   MVKQNNLFITVPTHFRCPISLDLMKSPVSLATGVTYDRSSIQRWLDSGNNTCPATMQLLL 60

Query: 100 NYDFVPNSNLKRLIQIWSDSL--QFPLSTLSQSDHILTAVDDLRTNGNGSVSVSVSVSAS 159
           + DFVPN NLKRLIQIWS +L  QFPL       H+ T    +R + + S SVSV    S
Sbjct: 61  SKDFVPNCNLKRLIQIWSHTLHFQFPL----HDHHLGTNATPIRNHSHPSCSVSV----S 120

Query: 160 LSKLLCFGARLEQNVKFLGRIHGFVPVLLDVL----RCRDVDCSELVVRVLDLVRCEIED 219
           LS LLCF   LEQNV+FL  IHG VPVLLDVL     C D++C EL V+VLDLVRCEIED
Sbjct: 121 LSNLLCFAPPLEQNVEFLRTIHGLVPVLLDVLGGEGSCGDIECFELAVKVLDLVRCEIED 180

Query: 220 REEFMNMMLKSDRDCLRSLVLVLQRGSSESRIGTARLLECVAINAESKNLIAENEAILHE 279
           REEFMN MLKS+RDCLRSL LVLQRGS+ESRIGTAR+LE VAINAESK LIAENE IL E
Sbjct: 181 REEFMNSMLKSNRDCLRSLALVLQRGSTESRIGTARILEYVAINAESKTLIAENEGILSE 240

Query: 280 LIGVIGIDEDPKLIESVLSCLIAISMPKRVKIKLVRLGVIKALTRLLKHRNASVGVTEKV 339
           LI +IG+D+D KLIESVLSCLIAISMP+RVKI+LV+LGVIKALTRLL  RNA+VGVTEKV
Sbjct: 241 LIALIGVDKDMKLIESVLSCLIAISMPRRVKIRLVQLGVIKALTRLLLQRNAAVGVTEKV 300

Query: 340 LRLLAAAAAVEEGRSEMLENGGECVGGMVKKVMKVSSAATEQAVTALWCICYLFREERAA 399
           +RLLAAAAAVEEGRSEM ENGGECVGGMV+KVMKVSSAATEQAVTALWCICYLFREERA 
Sbjct: 301 MRLLAAAAAVEEGRSEMCENGGECVGGMVRKVMKVSSAATEQAVTALWCICYLFREERAL 360

Query: 400 EAVAEAKGVEKILLLMQSHCPATVRQMAKDLLKILKVNSISSCLSIYDTNNSYIMPF 451
            AVAEAKGVEKILL++QS CPA VRQM KDLLKILKVNS S  LS YDTN+SYIMPF
Sbjct: 361 VAVAEAKGVEKILLVLQSECPAPVRQMGKDLLKILKVNSKSFTLSFYDTNSSYIMPF 409

BLAST of Cla97C02G026900 vs. NCBI nr
Match: KAG7035268.1 (U-box domain-containing protein 27, partial [Cucurbita argyrosperma subsp. argyrosperma])

HSP 1 Score: 585.1 bits (1507), Expect = 5.1e-163
Identity = 319/417 (76.50%), Postives = 353/417 (84.65%), Query Frame = 0

Query: 40  MVRPHNLFITVPTHFCCPISLDLMQSPVSLTTGVTYDRSSIQRWLDSGHNTCPATMQILH 99
           MV+ +NLFITVPTHF CPISLDLM+SPVSL TGVTYDRSSIQRWLDSG+NTCPATMQ+L 
Sbjct: 1   MVKQNNLFITVPTHFRCPISLDLMKSPVSLATGVTYDRSSIQRWLDSGNNTCPATMQLLL 60

Query: 100 NYDFVPNSNLKRLIQIWSDSL--QFPLSTLSQSDHILTAVDDLRTNGNGSVSVSVSVSAS 159
           + DFVPN NLKRLIQIWS +L  QFPL       H+ T    +R + +   SVSV    S
Sbjct: 61  SKDFVPNCNLKRLIQIWSHTLHFQFPL----HDHHLGTNATPIRNHSHPYCSVSV----S 120

Query: 160 LSKLLCFGARLEQNVKFLGRIHGFVPVLLDVL----RCRDVDCSELVVRVLDLVRCEIED 219
           LS LLCF   LEQNV+FL  IHG VPVLLDVL     C D++C EL V+VLDLVRCEI+D
Sbjct: 121 LSNLLCFSPPLEQNVEFLRTIHGLVPVLLDVLGGEGSCGDIECFELAVKVLDLVRCEIKD 180

Query: 220 REEFMNMMLKSDRDCLRSLVLVLQRGSSESRIGTARLLECVAINAESKNLIAENEAILHE 279
           REEFMN MLKS+RDCLRSL LVLQRG++ESRIGTAR+LEC+AINAESK LIAENE IL E
Sbjct: 181 REEFMNSMLKSNRDCLRSLALVLQRGNTESRIGTARILECIAINAESKTLIAENEGILCE 240

Query: 280 LIGVIGIDEDPKLIESVLSCLIAISMPKRVKIKLVRLGVIKALTRLLKHRNASVGVTEKV 339
           LI +IG+D+D KLIESVLSCL+AISMP+RVKI+LV+LGVIKALTRLL  RNA+VGVTEKV
Sbjct: 241 LIALIGVDKDMKLIESVLSCLVAISMPRRVKIRLVQLGVIKALTRLLLQRNAAVGVTEKV 300

Query: 340 LRLLAAAAAVEEGRSEMLENGGECVGGMVKKVMKVSSAATEQAVTALWCICYLFREERAA 399
           +RLLAAAAAVEEGRSEM ENGGECVGGMV+KVMKVSSAATEQAVTALWCICYLFREERA 
Sbjct: 301 MRLLAAAAAVEEGRSEMCENGGECVGGMVRKVMKVSSAATEQAVTALWCICYLFREERAL 360

Query: 400 EAVAEAKGVEKILLLMQSHCPATVRQMAKDLLKILKVNSISSCLSIYDTNNSYIMPF 451
            AVAEAKGVEKILL++QS CPA VRQM KDLLKILKVNS S  LS YDTN+SYIMPF
Sbjct: 361 VAVAEAKGVEKILLVLQSECPAPVRQMGKDLLKILKVNSKSFTLSFYDTNSSYIMPF 409

BLAST of Cla97C02G026900 vs. ExPASy Swiss-Prot
Match: Q9FLF4 (U-box domain-containing protein 27 OS=Arabidopsis thaliana OX=3702 GN=PUB27 PE=2 SV=1)

HSP 1 Score: 348.6 bits (893), Expect = 1.1e-94
Identity = 208/426 (48.83%), Postives = 276/426 (64.79%), Query Frame = 0

Query: 41  VRPHNLFITVPTHFCCPISLDLMQSPVSLTTGVTYDRSSIQRWLDSGHNTCPATMQILHN 100
           +R  +L ITVPT F CPISLD+M+SPVSL TGVTYDR+SIQRWLD G+NTCPATMQIL N
Sbjct: 1   MRKDDLCITVPTFFRCPISLDVMKSPVSLCTGVTYDRASIQRWLDGGNNTCPATMQILQN 60

Query: 101 YDFVPNSNLKRLIQIWSDSLQFPLSTLSQS------DHILTAVDDLRTNGNGSVSVSVSV 160
            DF+PN  L+RLI+IWSDS++      S        D I  A+D ++          V  
Sbjct: 61  KDFIPNRTLQRLIEIWSDSVRRRTCVESAELAAPTRDEIADAIDRVKIEKEERDDREV-- 120

Query: 161 SASLSKLLCFGARLEQNVKFLGRIHGFVPVLLDVLRCRDVDCS--------ELVVRVLDL 220
              LSK++ FG   + N  FL     FV +L+D++   D + +        +  V++L  
Sbjct: 121 ---LSKIVRFGRESDDNRGFLAGKDDFVKLLVDLINQVDFETTSAAKSLVVQEAVKILST 180

Query: 221 VRCEIEDREEFMNMMLKSDRDCLRSLVLVLQRGSSESRIGTARLLECVAINAESKNLIAE 280
           +R ++ DR  F N++L + RD L  +V + + G+ E +I  A LLE +A++AESK LIAE
Sbjct: 181 IRSKVSDRRRFSNLILTNGRDRLSVIVYLFKTGNVELKIDCAGLLEFIAVDAESKLLIAE 240

Query: 281 NEAILHELIGVIGIDEDPKLIESVLSCLIAISMPKRVKIKLVRLGVIKALTRLLKHRNA- 340
            + ++ EL+  I  D D  LIES LSCLIAIS PKRVK+ L+R  +I  +T+LL    + 
Sbjct: 241 RDGLITELMKSISKDSDLSLIESSLSCLIAISSPKRVKLNLLREKLIGDVTKLLSDSTSS 300

Query: 341 -SVGVTEKVLRLLAAAAAVEEGRSEMLENGGECVGGMVKKVMKVSSAATEQAVTALWCIC 400
            SV VTEK L+LL   A+ +EGRSE+    GEC+  +VKK+MKVS+AATE AVT LW + 
Sbjct: 301 LSVSVTEKCLKLLEILASTKEGRSEICGGDGECLKTVVKKLMKVSTAATEHAVTVLWSVS 360

Query: 401 YLFREERAAEAVAEAKGVEKILLLMQSHCPATVRQMAKDLLKILKVNSISSCLSIYDTNN 451
           YLF+E++A EAV    GV KILLL+QS+C   VR+M  DLLK+ KVNS  SCLS YDT  
Sbjct: 361 YLFKEDKALEAVTSVNGVTKILLLLQSNCSPAVRRMLTDLLKVFKVNS-RSCLSAYDTKT 420

BLAST of Cla97C02G026900 vs. ExPASy Swiss-Prot
Match: Q9LXE3 (U-box domain-containing protein 28 OS=Arabidopsis thaliana OX=3702 GN=PUB28 PE=1 SV=1)

HSP 1 Score: 334.7 bits (857), Expect = 1.6e-90
Identity = 200/423 (47.28%), Postives = 277/423 (65.48%), Query Frame = 0

Query: 41  VRPHNLFI-TVPTHFCCPISLDLMQSPVSLTTGVTYDRSSIQRWLDSGHNTCPATMQILH 100
           +R  +L+I TVP  F CPISLD+M+SPVSL+TGVTYDR SIQRWLD G+NTCPATMQIL 
Sbjct: 1   MRSDDLYITTVPCFFKCPISLDVMKSPVSLSTGVTYDRVSIQRWLDDGNNTCPATMQILQ 60

Query: 101 NYDFVPNSNLKRLIQIWSDSL------QFPLSTLSQSDHILTAVDDLRTNGNGSVSVSVS 160
           N +FVPN  L RLI  WSDS+      + P S     D I  A++  R   +        
Sbjct: 61  NKEFVPNLTLHRLIDHWSDSINRRADSESPESDTPTRDEINAAIERFRIEND-------- 120

Query: 161 VSASLSKLLCFGARLEQNVKFLGRIHGFVPVLLDVLR-CRDVDCSELV-----VRVLDLV 220
              + SK+L F    ++N +FL     FV +L+D++   R+   S+L+     V++L ++
Sbjct: 121 ---ARSKILRFARESDENREFLAGKDDFVAMLVDLISDSRNFSDSQLLLVGEAVKILSMI 180

Query: 221 RCEIEDREEFMNMMLKSDRDCLRSLVLVLQRGSSESRIGTARLLECVAINAESKNLIAEN 280
           R +I DR    N++L +  DCL S  L+++RG+ + +I  + +LE +A++AESK +IA+ 
Sbjct: 181 RRKIFDRRRLSNLILTNGGDCLTSFFLLIKRGNPKLKIDCSAVLEFIAVDAESKLIIAKG 240

Query: 281 EAILHELIGVIGIDEDPKLIESVLSCLIAISMPKRVKIKLVRLGVIKALTRLLKHRNASV 340
           E ++ E+I +I  D D  LIE+ LS LIAI+  KRVK+ L+R  ++  LT LL     SV
Sbjct: 241 EGLVTEIIKLISSDSDSSLIEANLSLLIAIASSKRVKLALIREKLVTKLTSLLTDPTTSV 300

Query: 341 GVTEKVLRLLAAAAAVEEGRSEMLENGGECVGGMVKKVMKVSSAATEQAVTALWCICYLF 400
            VTEK L+LL A ++ +EGRSE+ +  G CV  +V K+MKVS+AATE AVT LW +CYLF
Sbjct: 301 SVTEKCLKLLEAISSCKEGRSEICD--GVCVETVVNKLMKVSTAATEHAVTVLWSVCYLF 360

Query: 401 REERAAEAVAEAKGVEKILLLMQSHCPATVRQMAKDLLKILKVNSISSCLSIYDTNNSYI 451
           +E++A +AV    GV KILLL+QS+C  TVR M  DLLK+ KVNS  SCLS+Y+T  ++I
Sbjct: 361 KEKKAQDAVIRINGVTKILLLLQSNCSLTVRHMLTDLLKVFKVNS-RSCLSVYETKTTHI 409

BLAST of Cla97C02G026900 vs. ExPASy Swiss-Prot
Match: Q9LSA6 (U-box domain-containing protein 29 OS=Arabidopsis thaliana OX=3702 GN=PUB29 PE=1 SV=1)

HSP 1 Score: 314.7 bits (805), Expect = 1.7e-84
Identity = 192/418 (45.93%), Postives = 274/418 (65.55%), Query Frame = 0

Query: 47  FITVPTHFCCPISLDLMQSPVSLTTGVTYDRSSIQRWLDSGHNTCPATMQILHNYDFVPN 106
           +ITVP+ F CPISLD+M+SPVSL TGVTYDR+SIQRWLD G+NTCPATMQ+L   DFVPN
Sbjct: 9   YITVPSFFKCPISLDVMRSPVSLCTGVTYDRASIQRWLDGGNNTCPATMQLLKTKDFVPN 68

Query: 107 SNLKRLIQIWSDSLQFPLSTLSQSDHILTAVDDLRTNGNGSVSVSVSVSASLS---KLLC 166
             L+RLI IWSDS+       +    +L             V+V +    SL    K++ 
Sbjct: 69  LTLQRLINIWSDSIG---RRHNGDSPVLNPPSGREVPTKEEVNVLLERLMSLENLMKIVR 128

Query: 167 FGARLEQNVKFLGRIHGFVPVLLDVLRCR--DVDCSELVVRVLDLVRCEIEDREEFMNMM 226
           F    + N +FL +   FVP+L+D++R +   ++   + +R+LD ++    DRE   N+M
Sbjct: 129 FVKDSDSNREFLSKKMEFVPMLVDIIRTKKTKIELVIMAIRILDSIKV---DRERLSNLM 188

Query: 227 LKSD-RDCLRSLVLVLQRGSSESRIGTARLLECVAINAESKNLIAENEAILHELIGVIGI 286
           L +D  DCL +++L +QRG+ ES+I + R+L+ ++ +A+SK +IAE + +L E++  I I
Sbjct: 189 LANDGGDCLTAILLAIQRGNLESKIESVRVLDWISFDAKSKLMIAERDGVLTEMMKSISI 248

Query: 287 DE--DPKLIESVLSCLIAISMPKRVKIKLVRLGVIKALTR----LLKHRNASVGVTEKVL 346
            E  DP LIE+ LS LI IS  KRV+ KL+     KA+T+    LL     +V VTEK L
Sbjct: 249 TESSDPSLIEASLSFLITISKSKRVRSKLI---AAKAITKIKDILLTETLTNVAVTEKSL 308

Query: 347 RLLAAAAAVEEGRSEML-ENGGECVGGMVKKVMKVSSAATEQAVTALWCICYLFREERAA 406
           +LL   ++  EGR E+  ++ G CV G+VKK++KVS+ ATE AVT LWC+CY+FRE++  
Sbjct: 309 KLLETLSSKREGRLEICGDDNGRCVEGVVKKLLKVSTTATEHAVTILWCLCYVFREDKTV 368

Query: 407 EAVAE-AKGVEKILLLMQSHCPATVRQMAKDLLKILKVNSISSCLSIYDTNNSYIMPF 451
           E   E + GV K+L+++QS+C A VRQMAKDL+K+LK N  SS L+ Y+T  ++IMPF
Sbjct: 369 EETVERSNGVTKLLVVIQSNCSAMVRQMAKDLIKVLKFN--SSALAAYETKTTHIMPF 415

BLAST of Cla97C02G026900 vs. ExPASy Swiss-Prot
Match: Q9LT79 (U-box domain-containing protein 25 OS=Arabidopsis thaliana OX=3702 GN=PUB25 PE=2 SV=1)

HSP 1 Score: 128.3 bits (321), Expect = 2.3e-28
Identity = 125/406 (30.79%), Postives = 204/406 (50.25%), Query Frame = 0

Query: 41  VRPHNLFITVPTHFCCPISLDLMQSPVSLTTGVTYDRSSIQRWLDSGHN-TCPATMQILH 100
           + P +L I +P HF CPISL+LMQ PV++ TG TYDR+SI+ W+  G+N TCP T   L 
Sbjct: 5   IEPLDLGIQIPYHFRCPISLELMQDPVTVCTGQTYDRASIESWVSIGNNTTCPVTRAPLS 64

Query: 101 NYDFVPNSNLKRLIQIW-----SDSLQFPLSTLSQSDHILTAVDDLRTNGNGSVSVSVSV 160
           ++  +PN  L+RLIQ W     S+ ++   +    +D   T+V  L +  +      VSV
Sbjct: 65  DFTLIPNHTLRRLIQEWCVANRSNGVERIPTPKQPADP--TSVRALLSQASAITGTHVSV 124

Query: 161 ---SASLSKLLCFGARLEQNVKFLGRIHGFVPVLLDVLRCRDVDCSELVVRVLD-LVRCE 220
              +A+L +L  F    ++N + L   H    +L+ +L   +   SELV   L  LV   
Sbjct: 125 RSRAAALRRLRGFARDSDKN-RVLIAAHNATEILIKIL-FSETTSSELVSESLALLVMLP 184

Query: 221 IEDREEFMNMMLKSDRDCLRSLVLVLQRGSSESRIGTARLLECVAINAESKNL---IAEN 280
           I +  +F++  + SD   +  L  +L   S E+R+  A L+E V+   +S +L   I+ +
Sbjct: 185 ITEPNQFVS--ISSDPGRVEFLTRLLFDSSIETRVNAAALIEIVSTGTKSADLKGSISNS 244

Query: 281 EAILHELIGVIGIDEDP----KLIESVLSCLIAISMPKRVKIKLVRLGVIKALTRLLKHR 340
           E++     GV+ +  +P    + ++  +  L A+   K  +   +  G  + L   L   
Sbjct: 245 ESVFE---GVLDLLRNPISSRRALKIGIKTLFALCSVKSTRHIAITAGAPEILIDRLA-A 304

Query: 341 NASVGVTEKVLRLLAAAAAVEEGRSEMLENGGECVGGMVKKVMKVSSAATEQAVTALWCI 400
           +     TE+ L  +       EG +   E+    V  +VK +++VS  ATE A  AL  +
Sbjct: 305 DFDRCDTERALATVELLCRTPEGCAAFGEH-ALTVPLLVKTILRVSDRATEYAAGALLAL 364

Query: 401 CYLFREERAAEAVAEAKGVEKILLLMQSHCPATVRQMAKDLLKILK 430
           C    EER  E  A A  V ++LL++QS C    ++ A+ LLK+L+
Sbjct: 365 C--TAEERWREEAAGAGVVVQLLLMVQSECTERAKKKAQKLLKLLR 397

BLAST of Cla97C02G026900 vs. ExPASy Swiss-Prot
Match: Q84TG3 (E3 ubiquitin-protein ligase PUB23 OS=Arabidopsis thaliana OX=3702 GN=PUB23 PE=1 SV=1)

HSP 1 Score: 123.2 bits (308), Expect = 7.3e-27
Identity = 110/404 (27.23%), Postives = 191/404 (47.28%), Query Frame = 0

Query: 48  ITVPTHFCCPISLDLMQSPVSLTTGVTYDRSSIQRWLDSG-HNTCPATMQILHNYDFVPN 107
           I +P  F CPISL++M+ PV ++TG+TYDR SI++WL +G  N+CP T Q + + D  PN
Sbjct: 10  IEIPPFFLCPISLEIMKDPVIVSTGITYDRDSIEKWLFAGKKNSCPVTKQDITDADLTPN 69

Query: 108 SNLKRLIQIWS--------DSLQFPLSTLSQSDHILTAVDDLRTNGNGSVSVSVSVSASL 167
             L+RLIQ W         + +  P   + +S+     ++ L  +   S    V     L
Sbjct: 70  HTLRRLIQSWCTLNASYGVERIPTPRPPICKSE-----IEKLIRDSASSHENQVKCLKRL 129

Query: 168 SKLLCFGARLEQNVKFLGRIHGFVPVLLDVLRCRDVDCSELVVRVLDLVRCEIEDREEFM 227
            +++   A  ++ ++  G     VP  L  +   D +   L    L+L+   +E  E  +
Sbjct: 130 RQIVSENATNKRCLEAAG-----VPEFLANIVSNDSENGSLTDEALNLL-YHLETSETVL 189

Query: 228 NMML--KSDRDCLRSLVLVLQRGSSESRIGTARLLECVAINAESKNLIAENEAILHELIG 287
             +L  K D + ++SL  ++QRG  ESR+    LL+ +   A+    +     +  E++ 
Sbjct: 190 KNLLNNKKDNNIVKSLTKIMQRGMYESRVYATLLLKNILEVADPMQSMTLKPEVFTEVVQ 249

Query: 288 VIGIDEDPKLIESVLSCLIAISMPKRVKIKLVRLGVIKALTRLLKHRN--ASVGVTEKVL 347
           ++      K  ++ +  L+ I    R + K V  GVI  +  LL   +  +     E  +
Sbjct: 250 ILDDRISQKATKAAMHILVNICPWGRNRHKAVEAGVISVIIELLMDESFTSERRGPEMAM 309

Query: 348 RLLAAAAAVEEGRSEMLENGGECVGGMVKKVMKVSSAATEQAVTALWCICYLFREERAAE 407
            +L       EGR+E L N G  +  + KK+++VS  A+++AV  L  +           
Sbjct: 310 VVLDLLCQCAEGRAEFL-NHGAAIAVVCKKILRVSQTASDRAVRVLLSVGRFCATPALLH 369

Query: 408 AVAEAKGVEKILLLMQSHCPATVRQMAKDLLKI-LKVNSISSCL 438
            + +   V K+ L++Q  C    ++ AK+LLK+  +V   S CL
Sbjct: 370 EMLQLGVVAKLCLVLQVSCGGKTKEKAKELLKLHARVWKDSPCL 401

BLAST of Cla97C02G026900 vs. ExPASy TrEMBL
Match: A0A1S3CF07 (RING-type E3 ubiquitin transferase OS=Cucumis melo OX=3656 GN=LOC103499732 PE=4 SV=1)

HSP 1 Score: 647.9 bits (1670), Expect = 3.1e-182
Identity = 342/390 (87.69%), Postives = 356/390 (91.28%), Query Frame = 0

Query: 40  MVRPHNLFITVPTHFCCPISLDLMQSPVSLTTGVTYDRSSIQRWLDSGHNTCPATMQILH 99
           MV+PHNLF+T+PTHF CPISLDLMQSPVSL TGVTYDRSSIQRWLDSGHNTCPATMQ+L 
Sbjct: 1   MVKPHNLFLTIPTHFICPISLDLMQSPVSLITGVTYDRSSIQRWLDSGHNTCPATMQVLP 60

Query: 100 NYDFVPNSNLKRLIQIWSDSLQFPLSTLSQSDHILTAVDDLRTNGNGSVSVSVSVSASLS 159
           NY FVPNSNLKRLIQIWSDSL+F        DHILT VD LRTNGN SV         LS
Sbjct: 61  NYGFVPNSNLKRLIQIWSDSLEF--------DHILTVVDGLRTNGNASV--------FLS 120

Query: 160 KLLCFGARLEQNVKFLGRIHGFVPVLLDVLRCRDVDCSELVVRVLDLVRCEIEDREEFMN 219
           +LLCFG+RLE+NVKFLGRIHGFVPVLLDVLRCRDVDCSELVVRVLDLVRCEIEDR EFMN
Sbjct: 121 ELLCFGSRLEKNVKFLGRIHGFVPVLLDVLRCRDVDCSELVVRVLDLVRCEIEDRVEFMN 180

Query: 220 MMLKSDRDCLRSLVLVLQRGSSESRIGTARLLECVAINAESKNLIAENEAILHELIGVIG 279
           +MLKSDRDCL+SLVLVLQRGSSESRIGT RLLEC+AINAESKNLIAENE ILHELI VIG
Sbjct: 181 LMLKSDRDCLKSLVLVLQRGSSESRIGTVRLLECIAINAESKNLIAENEGILHELIXVIG 240

Query: 280 IDEDPKLIESVLSCLIAISMPKRVKIKLVRLGVIKALTRLLKHRNASVGVTEKVLRLLAA 339
           IDEDPKLIESVLSCLIAISMPKRVKIKLVRLGVIKALTRLLKHRNASVGVTEKVLRLLAA
Sbjct: 241 IDEDPKLIESVLSCLIAISMPKRVKIKLVRLGVIKALTRLLKHRNASVGVTEKVLRLLAA 300

Query: 340 AAAVEEGRSEMLENGGECVGGMVKKVMKVSSAATEQAVTALWCICYLFREERAAEAVAEA 399
           AAAVEEGRSEM+ NGGECVG MV+KVMKVSSAATEQAVTALWCICYLFREE+AA A AEA
Sbjct: 301 AAAVEEGRSEMMVNGGECVGRMVRKVMKVSSAATEQAVTALWCICYLFREEKAAVAAAEA 360

Query: 400 KGVEKILLLMQSHCPATVRQMAKDLLKILK 430
           KGVEKILLLMQSHCPATVR MAKDLLKI K
Sbjct: 361 KGVEKILLLMQSHCPATVRVMAKDLLKIFK 374

BLAST of Cla97C02G026900 vs. ExPASy TrEMBL
Match: A0A0A0LMY5 (RING-type E3 ubiquitin transferase OS=Cucumis sativus OX=3659 GN=Csa_2G302090 PE=4 SV=1)

HSP 1 Score: 644.0 bits (1660), Expect = 4.5e-181
Identity = 341/390 (87.44%), Postives = 354/390 (90.77%), Query Frame = 0

Query: 40  MVRPHNLFITVPTHFCCPISLDLMQSPVSLTTGVTYDRSSIQRWLDSGHNTCPATMQILH 99
           MV+PHNLFIT+PTHF CPISLDLMQSPVSL TGVTYDRSSIQRWLDSGHNTCPATMQ+L 
Sbjct: 1   MVKPHNLFITIPTHFLCPISLDLMQSPVSLITGVTYDRSSIQRWLDSGHNTCPATMQVLT 60

Query: 100 NYDFVPNSNLKRLIQIWSDSLQFPLSTLSQSDHILTAVDDLRTNGNGSVSVSVSVSASLS 159
           NYDFVPNSNLKRLIQIWSDSL+         DHILT VD LRTNGN SV        SLS
Sbjct: 61  NYDFVPNSNLKRLIQIWSDSLEL--------DHILTVVDGLRTNGNASV--------SLS 120

Query: 160 KLLCFGARLEQNVKFLGRIHGFVPVLLDVLRCRDVDCSELVVRVLDLVRCEIEDREEFMN 219
           +LLCFG+RLE+NVKFLGRI GFVPVLLDVLRCRDVDCSELVVRVLDLVRCEIEDR EFMN
Sbjct: 121 ELLCFGSRLEKNVKFLGRIRGFVPVLLDVLRCRDVDCSELVVRVLDLVRCEIEDRVEFMN 180

Query: 220 MMLKSDRDCLRSLVLVLQRGSSESRIGTARLLECVAINAESKNLIAENEAILHELIGVIG 279
           +MLKSDRDCLRSLVLVLQRGSSESRIGT RLLEC+AINAESKNLIAENE ILHELI VIG
Sbjct: 181 LMLKSDRDCLRSLVLVLQRGSSESRIGTVRLLECIAINAESKNLIAENEGILHELIEVIG 240

Query: 280 IDEDPKLIESVLSCLIAISMPKRVKIKLVRLGVIKALTRLLKHRNASVGVTEKVLRLLAA 339
           IDEDPKLIESVLSCLI ISMPKRVKIKLVRLGVIKALTRLLKH+NASVGVTEKVLRLLAA
Sbjct: 241 IDEDPKLIESVLSCLIPISMPKRVKIKLVRLGVIKALTRLLKHQNASVGVTEKVLRLLAA 300

Query: 340 AAAVEEGRSEMLENGGECVGGMVKKVMKVSSAATEQAVTALWCICYLFREERAAEAVAEA 399
           AAAVEEGR EM+ENGGECVG MV+KVMKVSSAATEQAVTALWCICYL+REERAA A AEA
Sbjct: 301 AAAVEEGRWEMMENGGECVGRMVRKVMKVSSAATEQAVTALWCICYLYREERAAVAAAEA 360

Query: 400 KGVEKILLLMQSHCPATVRQMAKDLLKILK 430
           KGVEKILLLMQSHCPATVR MAKDLLK  K
Sbjct: 361 KGVEKILLLMQSHCPATVRAMAKDLLKTFK 374

BLAST of Cla97C02G026900 vs. ExPASy TrEMBL
Match: A0A6J1L488 (RING-type E3 ubiquitin transferase OS=Cucurbita maxima OX=3661 GN=LOC111499751 PE=4 SV=1)

HSP 1 Score: 584.7 bits (1506), Expect = 3.3e-163
Identity = 321/417 (76.98%), Postives = 352/417 (84.41%), Query Frame = 0

Query: 40  MVRPHNLFITVPTHFCCPISLDLMQSPVSLTTGVTYDRSSIQRWLDSGHNTCPATMQILH 99
           MV+ +NLFITVPTHF CPISLDLM+SPVSL TGVTYDRSSIQRWLDSG+NTCPATMQ+L 
Sbjct: 1   MVKQNNLFITVPTHFRCPISLDLMKSPVSLATGVTYDRSSIQRWLDSGNNTCPATMQLLL 60

Query: 100 NYDFVPNSNLKRLIQIWSDS--LQFPLSTLSQSDHILTAVDDLRTNGNGSVSVSVSVSAS 159
           + DFVPN NLKRLIQIWS +   QFPL       H+ T    +R + + S SVSV    S
Sbjct: 61  SKDFVPNCNLKRLIQIWSHTFHFQFPL----HDHHLGTNAAPIRNHAHPSCSVSV----S 120

Query: 160 LSKLLCFGARLEQNVKFLGRIHGFVPVLLDVL----RCRDVDCSELVVRVLDLVRCEIED 219
           LS LLCF   LEQNV+FL  IHG VPVLLDVL     C D++C EL V+VLDLVRCEIED
Sbjct: 121 LSNLLCFAPPLEQNVEFLRTIHGLVPVLLDVLGEEGSCGDIECFELAVKVLDLVRCEIED 180

Query: 220 REEFMNMMLKSDRDCLRSLVLVLQRGSSESRIGTARLLECVAINAESKNLIAENEAILHE 279
           REEFM  MLKS+R+CLRSL LVLQRGS+ESRIGTAR+LECVAINAESK LIAENE IL E
Sbjct: 181 REEFMTSMLKSNRNCLRSLALVLQRGSTESRIGTARILECVAINAESKTLIAENEGILCE 240

Query: 280 LIGVIGIDEDPKLIESVLSCLIAISMPKRVKIKLVRLGVIKALTRLLKHRNASVGVTEKV 339
           LI +IG+D+D KLIESVLSCLIAISMP+RVKI+LV+LGVIKALTRLL  RNA+VGVTEKV
Sbjct: 241 LIALIGVDKDMKLIESVLSCLIAISMPRRVKIRLVQLGVIKALTRLLLQRNAAVGVTEKV 300

Query: 340 LRLLAAAAAVEEGRSEMLENGGECVGGMVKKVMKVSSAATEQAVTALWCICYLFREERAA 399
           +RLLAAAAAVEEGRSEM ENGGECVGGMV+KVMKVSSAATEQAVTALWCICYLFREERA 
Sbjct: 301 MRLLAAAAAVEEGRSEMCENGGECVGGMVRKVMKVSSAATEQAVTALWCICYLFREERAL 360

Query: 400 EAVAEAKGVEKILLLMQSHCPATVRQMAKDLLKILKVNSISSCLSIYDTNNSYIMPF 451
            AVAEAKGVEKILL++QS CPA VRQM KDLLKILKVNS S  LS YDTN+SYIMPF
Sbjct: 361 VAVAEAKGVEKILLVLQSECPAPVRQMGKDLLKILKVNSKSFTLSFYDTNSSYIMPF 409

BLAST of Cla97C02G026900 vs. ExPASy TrEMBL
Match: A0A6J1G743 (RING-type E3 ubiquitin transferase OS=Cucurbita moschata OX=3662 GN=LOC111451471 PE=4 SV=1)

HSP 1 Score: 583.2 bits (1502), Expect = 9.5e-163
Identity = 320/417 (76.74%), Postives = 351/417 (84.17%), Query Frame = 0

Query: 40  MVRPHNLFITVPTHFCCPISLDLMQSPVSLTTGVTYDRSSIQRWLDSGHNTCPATMQILH 99
           MV+ +NLFITVPTHF CPISLDLM+SPVSL TGVTYDRSSIQRWLDSG+NTCPATMQ+L 
Sbjct: 1   MVKQNNLFITVPTHFRCPISLDLMKSPVSLATGVTYDRSSIQRWLDSGNNTCPATMQLLL 60

Query: 100 NYDFVPNSNLKRLIQIWSDSL--QFPLSTLSQSDHILTAVDDLRTNGNGSVSVSVSVSAS 159
           + DFVPN NLKRLIQIWS +L  QFPL       H+ T    +R + + S SVSV    S
Sbjct: 61  SKDFVPNCNLKRLIQIWSHTLHFQFPL----HDHHLGTNATPIRNHSHPSCSVSV----S 120

Query: 160 LSKLLCFGARLEQNVKFLGRIHGFVPVLLDVL----RCRDVDCSELVVRVLDLVRCEIED 219
           LS LLCF   LEQNV+FL  I G VPVLLDVL     C D++C EL V+VLDLVRCEIED
Sbjct: 121 LSNLLCFAPPLEQNVEFLRTIQGLVPVLLDVLGGEGSCGDIECFELAVKVLDLVRCEIED 180

Query: 220 REEFMNMMLKSDRDCLRSLVLVLQRGSSESRIGTARLLECVAINAESKNLIAENEAILHE 279
           REEFMN MLKS+RDCLRSL LVLQRGS+ESRIGTAR+LECVAINAESK LIAENE IL E
Sbjct: 181 REEFMNSMLKSNRDCLRSLALVLQRGSTESRIGTARILECVAINAESKTLIAENEGILCE 240

Query: 280 LIGVIGIDEDPKLIESVLSCLIAISMPKRVKIKLVRLGVIKALTRLLKHRNASVGVTEKV 339
           LI +IG+D+D KLIESVLSCL+AISMP+RVKI+LV+LGVIKALTRLL  RN +VGVTEKV
Sbjct: 241 LIALIGVDKDMKLIESVLSCLVAISMPRRVKIRLVQLGVIKALTRLLLQRNVAVGVTEKV 300

Query: 340 LRLLAAAAAVEEGRSEMLENGGECVGGMVKKVMKVSSAATEQAVTALWCICYLFREERAA 399
           +RLLAAAAAV EGRSEM ENGGECVGGMV+KVMKVSSAATEQAVTALWCICYLFREERA 
Sbjct: 301 MRLLAAAAAVVEGRSEMCENGGECVGGMVRKVMKVSSAATEQAVTALWCICYLFREERAL 360

Query: 400 EAVAEAKGVEKILLLMQSHCPATVRQMAKDLLKILKVNSISSCLSIYDTNNSYIMPF 451
            AVAEAKGVEKILL++QS CPA VRQM KDLLKILKVNS S  LS YDTN+SYIMPF
Sbjct: 361 VAVAEAKGVEKILLVLQSECPAPVRQMGKDLLKILKVNSKSFTLSFYDTNSSYIMPF 409

BLAST of Cla97C02G026900 vs. ExPASy TrEMBL
Match: A0A438DBD3 (RING-type E3 ubiquitin transferase OS=Vitis vinifera OX=29760 GN=PUB27_1 PE=4 SV=1)

HSP 1 Score: 406.8 bits (1044), Expect = 1.2e-109
Identity = 227/415 (54.70%), Postives = 301/415 (72.53%), Query Frame = 0

Query: 40  MVRPHNLFITVPTHFCCPISLDLMQSPVSLTTGVTYDRSSIQRWLDSGHNTCPATMQILH 99
           MVR  NLFITVP+ F CPISLD+M+SPVSL TGVTYDRSSIQRWLD+G+NTCPATMQ+LH
Sbjct: 1   MVR-DNLFITVPSLFRCPISLDVMKSPVSLCTGVTYDRSSIQRWLDNGNNTCPATMQVLH 60

Query: 100 NYDFVPNSNLKRLIQIWSDSLQFPLSTLSQSDHILTAV--DDLRTNGNGSVSVSVSVSAS 159
           + DFVPN  L+RLIQIWS+S++   ++      ++ ++  D  R       S        
Sbjct: 61  SKDFVPNHTLQRLIQIWSNSVRHRSNSPDSPIQLVPSLSPDQARDLIKEIESKPEDCLEC 120

Query: 160 LSKLLCFGARLEQNVKFLGRIHGFVPVLLDVLRCRDVD--CSELVVRVLDLVRCEIEDRE 219
           +SK++CF    E++ KFL RI GFV +L+D L   + +    E VVRVLD++  E ED +
Sbjct: 121 MSKIICFARESEESRKFLARIDGFVSLLVDFLGSGNANFLALEQVVRVLDMIISEHEDHK 180

Query: 220 EFMNMMLKSDRDCLRSLVLVLQRGSSESRIGTARLLECVAINAESKNLIAENEAILHELI 279
           +  N MLKSDRDCL S++LVLQ+GS+ESRI +AR+LE +AI+AESK LIAE + +  ELI
Sbjct: 181 QLANSMLKSDRDCLSSILLVLQQGSAESRIASARVLESIAIDAESKLLIAEKDGLFSELI 240

Query: 280 GVIGIDEDPKLIESVLSCLIAISMPKRVKIKLVRLGVIKALTRLLKHRNASVGVTEKVLR 339
            ++  + DP +IES LSCLIA+SMP+R++ K+VRLGV+K LT+LL   N SV VTEK L+
Sbjct: 241 RIMSTETDPTMIESTLSCLIAVSMPRRIRPKIVRLGVVKQLTKLLSDPNWSVSVTEKALK 300

Query: 340 LLAAAAAVEEGRSEMLENGGECVGGMVKKVMKVSSAATEQAVTALWCICYLFREERAAEA 399
           LL  A++ +EG+SE+ EN  +CV  +V+K++KVSS ATE AVT LW +CYL R++RA   
Sbjct: 301 LLEMASSCKEGKSEICEN-SDCVSAIVQKMLKVSSTATEHAVTILWSVCYLSRDDRAQST 360

Query: 400 VAEAKGVEKILLLMQSHCPATVRQMAKDLLKILKVNSISSCLSIYDTNNSYIMPF 451
           V +  G+ KIL+LMQS+C   VRQ+A DLLKI +V+S  SCLS YDT  ++IMPF
Sbjct: 361 VTQNNGLTKILVLMQSNCSPAVRQLAGDLLKIFRVSS-KSCLSSYDTKTTHIMPF 412

BLAST of Cla97C02G026900 vs. TAIR 10
Match: AT5G64660.1 (CYS, MET, PRO, and GLY protein 2 )

HSP 1 Score: 348.6 bits (893), Expect = 7.5e-96
Identity = 208/426 (48.83%), Postives = 276/426 (64.79%), Query Frame = 0

Query: 41  VRPHNLFITVPTHFCCPISLDLMQSPVSLTTGVTYDRSSIQRWLDSGHNTCPATMQILHN 100
           +R  +L ITVPT F CPISLD+M+SPVSL TGVTYDR+SIQRWLD G+NTCPATMQIL N
Sbjct: 1   MRKDDLCITVPTFFRCPISLDVMKSPVSLCTGVTYDRASIQRWLDGGNNTCPATMQILQN 60

Query: 101 YDFVPNSNLKRLIQIWSDSLQFPLSTLSQS------DHILTAVDDLRTNGNGSVSVSVSV 160
            DF+PN  L+RLI+IWSDS++      S        D I  A+D ++          V  
Sbjct: 61  KDFIPNRTLQRLIEIWSDSVRRRTCVESAELAAPTRDEIADAIDRVKIEKEERDDREV-- 120

Query: 161 SASLSKLLCFGARLEQNVKFLGRIHGFVPVLLDVLRCRDVDCS--------ELVVRVLDL 220
              LSK++ FG   + N  FL     FV +L+D++   D + +        +  V++L  
Sbjct: 121 ---LSKIVRFGRESDDNRGFLAGKDDFVKLLVDLINQVDFETTSAAKSLVVQEAVKILST 180

Query: 221 VRCEIEDREEFMNMMLKSDRDCLRSLVLVLQRGSSESRIGTARLLECVAINAESKNLIAE 280
           +R ++ DR  F N++L + RD L  +V + + G+ E +I  A LLE +A++AESK LIAE
Sbjct: 181 IRSKVSDRRRFSNLILTNGRDRLSVIVYLFKTGNVELKIDCAGLLEFIAVDAESKLLIAE 240

Query: 281 NEAILHELIGVIGIDEDPKLIESVLSCLIAISMPKRVKIKLVRLGVIKALTRLLKHRNA- 340
            + ++ EL+  I  D D  LIES LSCLIAIS PKRVK+ L+R  +I  +T+LL    + 
Sbjct: 241 RDGLITELMKSISKDSDLSLIESSLSCLIAISSPKRVKLNLLREKLIGDVTKLLSDSTSS 300

Query: 341 -SVGVTEKVLRLLAAAAAVEEGRSEMLENGGECVGGMVKKVMKVSSAATEQAVTALWCIC 400
            SV VTEK L+LL   A+ +EGRSE+    GEC+  +VKK+MKVS+AATE AVT LW + 
Sbjct: 301 LSVSVTEKCLKLLEILASTKEGRSEICGGDGECLKTVVKKLMKVSTAATEHAVTVLWSVS 360

Query: 401 YLFREERAAEAVAEAKGVEKILLLMQSHCPATVRQMAKDLLKILKVNSISSCLSIYDTNN 451
           YLF+E++A EAV    GV KILLL+QS+C   VR+M  DLLK+ KVNS  SCLS YDT  
Sbjct: 361 YLFKEDKALEAVTSVNGVTKILLLLQSNCSPAVRRMLTDLLKVFKVNS-RSCLSAYDTKT 420

BLAST of Cla97C02G026900 vs. TAIR 10
Match: AT5G09800.1 (ARM repeat superfamily protein )

HSP 1 Score: 334.7 bits (857), Expect = 1.1e-91
Identity = 200/423 (47.28%), Postives = 277/423 (65.48%), Query Frame = 0

Query: 41  VRPHNLFI-TVPTHFCCPISLDLMQSPVSLTTGVTYDRSSIQRWLDSGHNTCPATMQILH 100
           +R  +L+I TVP  F CPISLD+M+SPVSL+TGVTYDR SIQRWLD G+NTCPATMQIL 
Sbjct: 1   MRSDDLYITTVPCFFKCPISLDVMKSPVSLSTGVTYDRVSIQRWLDDGNNTCPATMQILQ 60

Query: 101 NYDFVPNSNLKRLIQIWSDSL------QFPLSTLSQSDHILTAVDDLRTNGNGSVSVSVS 160
           N +FVPN  L RLI  WSDS+      + P S     D I  A++  R   +        
Sbjct: 61  NKEFVPNLTLHRLIDHWSDSINRRADSESPESDTPTRDEINAAIERFRIEND-------- 120

Query: 161 VSASLSKLLCFGARLEQNVKFLGRIHGFVPVLLDVLR-CRDVDCSELV-----VRVLDLV 220
              + SK+L F    ++N +FL     FV +L+D++   R+   S+L+     V++L ++
Sbjct: 121 ---ARSKILRFARESDENREFLAGKDDFVAMLVDLISDSRNFSDSQLLLVGEAVKILSMI 180

Query: 221 RCEIEDREEFMNMMLKSDRDCLRSLVLVLQRGSSESRIGTARLLECVAINAESKNLIAEN 280
           R +I DR    N++L +  DCL S  L+++RG+ + +I  + +LE +A++AESK +IA+ 
Sbjct: 181 RRKIFDRRRLSNLILTNGGDCLTSFFLLIKRGNPKLKIDCSAVLEFIAVDAESKLIIAKG 240

Query: 281 EAILHELIGVIGIDEDPKLIESVLSCLIAISMPKRVKIKLVRLGVIKALTRLLKHRNASV 340
           E ++ E+I +I  D D  LIE+ LS LIAI+  KRVK+ L+R  ++  LT LL     SV
Sbjct: 241 EGLVTEIIKLISSDSDSSLIEANLSLLIAIASSKRVKLALIREKLVTKLTSLLTDPTTSV 300

Query: 341 GVTEKVLRLLAAAAAVEEGRSEMLENGGECVGGMVKKVMKVSSAATEQAVTALWCICYLF 400
            VTEK L+LL A ++ +EGRSE+ +  G CV  +V K+MKVS+AATE AVT LW +CYLF
Sbjct: 301 SVTEKCLKLLEAISSCKEGRSEICD--GVCVETVVNKLMKVSTAATEHAVTVLWSVCYLF 360

Query: 401 REERAAEAVAEAKGVEKILLLMQSHCPATVRQMAKDLLKILKVNSISSCLSIYDTNNSYI 451
           +E++A +AV    GV KILLL+QS+C  TVR M  DLLK+ KVNS  SCLS+Y+T  ++I
Sbjct: 361 KEKKAQDAVIRINGVTKILLLLQSNCSLTVRHMLTDLLKVFKVNS-RSCLSVYETKTTHI 409

BLAST of Cla97C02G026900 vs. TAIR 10
Match: AT3G18710.1 (plant U-box 29 )

HSP 1 Score: 314.7 bits (805), Expect = 1.2e-85
Identity = 192/418 (45.93%), Postives = 274/418 (65.55%), Query Frame = 0

Query: 47  FITVPTHFCCPISLDLMQSPVSLTTGVTYDRSSIQRWLDSGHNTCPATMQILHNYDFVPN 106
           +ITVP+ F CPISLD+M+SPVSL TGVTYDR+SIQRWLD G+NTCPATMQ+L   DFVPN
Sbjct: 9   YITVPSFFKCPISLDVMRSPVSLCTGVTYDRASIQRWLDGGNNTCPATMQLLKTKDFVPN 68

Query: 107 SNLKRLIQIWSDSLQFPLSTLSQSDHILTAVDDLRTNGNGSVSVSVSVSASLS---KLLC 166
             L+RLI IWSDS+       +    +L             V+V +    SL    K++ 
Sbjct: 69  LTLQRLINIWSDSIG---RRHNGDSPVLNPPSGREVPTKEEVNVLLERLMSLENLMKIVR 128

Query: 167 FGARLEQNVKFLGRIHGFVPVLLDVLRCR--DVDCSELVVRVLDLVRCEIEDREEFMNMM 226
           F    + N +FL +   FVP+L+D++R +   ++   + +R+LD ++    DRE   N+M
Sbjct: 129 FVKDSDSNREFLSKKMEFVPMLVDIIRTKKTKIELVIMAIRILDSIKV---DRERLSNLM 188

Query: 227 LKSD-RDCLRSLVLVLQRGSSESRIGTARLLECVAINAESKNLIAENEAILHELIGVIGI 286
           L +D  DCL +++L +QRG+ ES+I + R+L+ ++ +A+SK +IAE + +L E++  I I
Sbjct: 189 LANDGGDCLTAILLAIQRGNLESKIESVRVLDWISFDAKSKLMIAERDGVLTEMMKSISI 248

Query: 287 DE--DPKLIESVLSCLIAISMPKRVKIKLVRLGVIKALTR----LLKHRNASVGVTEKVL 346
            E  DP LIE+ LS LI IS  KRV+ KL+     KA+T+    LL     +V VTEK L
Sbjct: 249 TESSDPSLIEASLSFLITISKSKRVRSKLI---AAKAITKIKDILLTETLTNVAVTEKSL 308

Query: 347 RLLAAAAAVEEGRSEML-ENGGECVGGMVKKVMKVSSAATEQAVTALWCICYLFREERAA 406
           +LL   ++  EGR E+  ++ G CV G+VKK++KVS+ ATE AVT LWC+CY+FRE++  
Sbjct: 309 KLLETLSSKREGRLEICGDDNGRCVEGVVKKLLKVSTTATEHAVTILWCLCYVFREDKTV 368

Query: 407 EAVAE-AKGVEKILLLMQSHCPATVRQMAKDLLKILKVNSISSCLSIYDTNNSYIMPF 451
           E   E + GV K+L+++QS+C A VRQMAKDL+K+LK N  SS L+ Y+T  ++IMPF
Sbjct: 369 EETVERSNGVTKLLVVIQSNCSAMVRQMAKDLIKVLKFN--SSALAAYETKTTHIMPF 415

BLAST of Cla97C02G026900 vs. TAIR 10
Match: AT3G19380.1 (plant U-box 25 )

HSP 1 Score: 128.3 bits (321), Expect = 1.6e-29
Identity = 125/406 (30.79%), Postives = 204/406 (50.25%), Query Frame = 0

Query: 41  VRPHNLFITVPTHFCCPISLDLMQSPVSLTTGVTYDRSSIQRWLDSGHN-TCPATMQILH 100
           + P +L I +P HF CPISL+LMQ PV++ TG TYDR+SI+ W+  G+N TCP T   L 
Sbjct: 5   IEPLDLGIQIPYHFRCPISLELMQDPVTVCTGQTYDRASIESWVSIGNNTTCPVTRAPLS 64

Query: 101 NYDFVPNSNLKRLIQIW-----SDSLQFPLSTLSQSDHILTAVDDLRTNGNGSVSVSVSV 160
           ++  +PN  L+RLIQ W     S+ ++   +    +D   T+V  L +  +      VSV
Sbjct: 65  DFTLIPNHTLRRLIQEWCVANRSNGVERIPTPKQPADP--TSVRALLSQASAITGTHVSV 124

Query: 161 ---SASLSKLLCFGARLEQNVKFLGRIHGFVPVLLDVLRCRDVDCSELVVRVLD-LVRCE 220
              +A+L +L  F    ++N + L   H    +L+ +L   +   SELV   L  LV   
Sbjct: 125 RSRAAALRRLRGFARDSDKN-RVLIAAHNATEILIKIL-FSETTSSELVSESLALLVMLP 184

Query: 221 IEDREEFMNMMLKSDRDCLRSLVLVLQRGSSESRIGTARLLECVAINAESKNL---IAEN 280
           I +  +F++  + SD   +  L  +L   S E+R+  A L+E V+   +S +L   I+ +
Sbjct: 185 ITEPNQFVS--ISSDPGRVEFLTRLLFDSSIETRVNAAALIEIVSTGTKSADLKGSISNS 244

Query: 281 EAILHELIGVIGIDEDP----KLIESVLSCLIAISMPKRVKIKLVRLGVIKALTRLLKHR 340
           E++     GV+ +  +P    + ++  +  L A+   K  +   +  G  + L   L   
Sbjct: 245 ESVFE---GVLDLLRNPISSRRALKIGIKTLFALCSVKSTRHIAITAGAPEILIDRLA-A 304

Query: 341 NASVGVTEKVLRLLAAAAAVEEGRSEMLENGGECVGGMVKKVMKVSSAATEQAVTALWCI 400
           +     TE+ L  +       EG +   E+    V  +VK +++VS  ATE A  AL  +
Sbjct: 305 DFDRCDTERALATVELLCRTPEGCAAFGEH-ALTVPLLVKTILRVSDRATEYAAGALLAL 364

Query: 401 CYLFREERAAEAVAEAKGVEKILLLMQSHCPATVRQMAKDLLKILK 430
           C    EER  E  A A  V ++LL++QS C    ++ A+ LLK+L+
Sbjct: 365 C--TAEERWREEAAGAGVVVQLLLMVQSECTERAKKKAQKLLKLLR 397

BLAST of Cla97C02G026900 vs. TAIR 10
Match: AT2G35930.1 (plant U-box 23 )

HSP 1 Score: 123.2 bits (308), Expect = 5.2e-28
Identity = 110/404 (27.23%), Postives = 191/404 (47.28%), Query Frame = 0

Query: 48  ITVPTHFCCPISLDLMQSPVSLTTGVTYDRSSIQRWLDSG-HNTCPATMQILHNYDFVPN 107
           I +P  F CPISL++M+ PV ++TG+TYDR SI++WL +G  N+CP T Q + + D  PN
Sbjct: 10  IEIPPFFLCPISLEIMKDPVIVSTGITYDRDSIEKWLFAGKKNSCPVTKQDITDADLTPN 69

Query: 108 SNLKRLIQIWS--------DSLQFPLSTLSQSDHILTAVDDLRTNGNGSVSVSVSVSASL 167
             L+RLIQ W         + +  P   + +S+     ++ L  +   S    V     L
Sbjct: 70  HTLRRLIQSWCTLNASYGVERIPTPRPPICKSE-----IEKLIRDSASSHENQVKCLKRL 129

Query: 168 SKLLCFGARLEQNVKFLGRIHGFVPVLLDVLRCRDVDCSELVVRVLDLVRCEIEDREEFM 227
            +++   A  ++ ++  G     VP  L  +   D +   L    L+L+   +E  E  +
Sbjct: 130 RQIVSENATNKRCLEAAG-----VPEFLANIVSNDSENGSLTDEALNLL-YHLETSETVL 189

Query: 228 NMML--KSDRDCLRSLVLVLQRGSSESRIGTARLLECVAINAESKNLIAENEAILHELIG 287
             +L  K D + ++SL  ++QRG  ESR+    LL+ +   A+    +     +  E++ 
Sbjct: 190 KNLLNNKKDNNIVKSLTKIMQRGMYESRVYATLLLKNILEVADPMQSMTLKPEVFTEVVQ 249

Query: 288 VIGIDEDPKLIESVLSCLIAISMPKRVKIKLVRLGVIKALTRLLKHRN--ASVGVTEKVL 347
           ++      K  ++ +  L+ I    R + K V  GVI  +  LL   +  +     E  +
Sbjct: 250 ILDDRISQKATKAAMHILVNICPWGRNRHKAVEAGVISVIIELLMDESFTSERRGPEMAM 309

Query: 348 RLLAAAAAVEEGRSEMLENGGECVGGMVKKVMKVSSAATEQAVTALWCICYLFREERAAE 407
            +L       EGR+E L N G  +  + KK+++VS  A+++AV  L  +           
Sbjct: 310 VVLDLLCQCAEGRAEFL-NHGAAIAVVCKKILRVSQTASDRAVRVLLSVGRFCATPALLH 369

Query: 408 AVAEAKGVEKILLLMQSHCPATVRQMAKDLLKI-LKVNSISSCL 438
            + +   V K+ L++Q  C    ++ AK+LLK+  +V   S CL
Sbjct: 370 EMLQLGVVAKLCLVLQVSCGGKTKEKAKELLKLHARVWKDSPCL 401

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
XP_038901738.12.0e-19991.22U-box domain-containing protein 27-like [Benincasa hispida][more]
XP_008461027.16.5e-18287.69PREDICTED: LOW QUALITY PROTEIN: U-box domain-containing protein 27 [Cucumis melo... [more]
XP_004147378.19.3e-18187.44U-box domain-containing protein 27 [Cucumis sativus] >KGN62142.1 hypothetical pr... [more]
XP_023534066.11.0e-16377.46U-box domain-containing protein 27-like [Cucurbita pepo subsp. pepo][more]
KAG7035268.15.1e-16376.50U-box domain-containing protein 27, partial [Cucurbita argyrosperma subsp. argyr... [more]
Match NameE-valueIdentityDescription
Q9FLF41.1e-9448.83U-box domain-containing protein 27 OS=Arabidopsis thaliana OX=3702 GN=PUB27 PE=2... [more]
Q9LXE31.6e-9047.28U-box domain-containing protein 28 OS=Arabidopsis thaliana OX=3702 GN=PUB28 PE=1... [more]
Q9LSA61.7e-8445.93U-box domain-containing protein 29 OS=Arabidopsis thaliana OX=3702 GN=PUB29 PE=1... [more]
Q9LT792.3e-2830.79U-box domain-containing protein 25 OS=Arabidopsis thaliana OX=3702 GN=PUB25 PE=2... [more]
Q84TG37.3e-2727.23E3 ubiquitin-protein ligase PUB23 OS=Arabidopsis thaliana OX=3702 GN=PUB23 PE=1 ... [more]
Match NameE-valueIdentityDescription
A0A1S3CF073.1e-18287.69RING-type E3 ubiquitin transferase OS=Cucumis melo OX=3656 GN=LOC103499732 PE=4 ... [more]
A0A0A0LMY54.5e-18187.44RING-type E3 ubiquitin transferase OS=Cucumis sativus OX=3659 GN=Csa_2G302090 PE... [more]
A0A6J1L4883.3e-16376.98RING-type E3 ubiquitin transferase OS=Cucurbita maxima OX=3661 GN=LOC111499751 P... [more]
A0A6J1G7439.5e-16376.74RING-type E3 ubiquitin transferase OS=Cucurbita moschata OX=3662 GN=LOC111451471... [more]
A0A438DBD31.2e-10954.70RING-type E3 ubiquitin transferase OS=Vitis vinifera OX=29760 GN=PUB27_1 PE=4 SV... [more]
Match NameE-valueIdentityDescription
AT5G64660.17.5e-9648.83CYS, MET, PRO, and GLY protein 2 [more]
AT5G09800.11.1e-9147.28ARM repeat superfamily protein [more]
AT3G18710.11.2e-8545.93plant U-box 29 [more]
AT3G19380.11.6e-2930.79plant U-box 25 [more]
AT2G35930.15.2e-2827.23plant U-box 23 [more]
InterPro
Analysis Name: InterPro Annotations of Watermelon (97103) v2.5
Date Performed: 2022-01-31
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR003613U box domainSMARTSM00504Ubox_2coord: 53..116
e-value: 6.8E-24
score: 95.4
IPR003613U box domainPFAMPF04564U-boxcoord: 50..117
e-value: 1.4E-18
score: 66.8
IPR003613U box domainPROSITEPS51698U_BOXcoord: 49..123
score: 34.261162
IPR013083Zinc finger, RING/FYVE/PHD-typeGENE3D3.30.40.10Zinc/RING finger domain, C3HC4 (zinc finger)coord: 49..122
e-value: 4.4E-25
score: 89.5
IPR011989Armadillo-like helicalGENE3D1.25.10.10coord: 124..440
e-value: 2.3E-18
score: 68.0
NoneNo IPR availablePANTHERPTHR22849:SF20U-BOX DOMAIN-CONTAINING PROTEIN 27-RELATEDcoord: 44..450
NoneNo IPR availablePANTHERPTHR22849WDSAM1 PROTEINcoord: 44..450
NoneNo IPR availableCDDcd16664RING-Ubox_PUBcoord: 54..96
e-value: 7.60849E-24
score: 91.5262
NoneNo IPR availableSUPERFAMILY57850RING/U-boxcoord: 47..118
IPR000225ArmadilloPROSITEPS50176ARM_REPEATcoord: 311..355
score: 9.3974
IPR016024Armadillo-type foldSUPERFAMILY48371ARM repeatcoord: 166..439

Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
Cla97C02G026900.2Cla97C02G026900.2mRNA


GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
biological_process GO:0016567 protein ubiquitination
molecular_function GO:0005515 protein binding
molecular_function GO:0004842 ubiquitin-protein transferase activity