Cla97C01G024660 (gene) Watermelon (97103) v2.5

Overview
NameCla97C01G024660
Typegene
OrganismCitrullus lanatus subsp. vulgaris cv. 97103 (Watermelon (97103) v2.5)
Description(S)-2-hydroxy-acid oxidase
LocationCla97Chr01: 35716118 .. 35720412 (-)
RNA-Seq ExpressionCla97C01G024660
SyntenyCla97C01G024660
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: exonfive_prime_UTRpolypeptideCDSthree_prime_UTR
Hold the cursor over a type above to highlight its positions in the sequence below.
CAAATTCGTCAACACCATTTTTTCCTTCATCCCAAGCAATTCGAATGGAATTTAGATTACATTATTGAGGATTGAGAATGAGCGACAGTCTTGCGAACGCCCTCCACACAAGTTTTTATTTCTGATTAATATGGGAAATTTCTGTGAAACAGATTTGGTTTCTAACGCTCACCTTCAAAATCCAATCCAAATAATGTGGTCTCTCTCTCTTTTCCCCAATTTGATCCCACCCCTTTTGTCTTTACCTTCAATCCGATCACGGTCTTCCTCTATCGCCCTTATGCTATATTTGTTGTTTGTTTTCGCATTCCCCATTTCGAGAGCTCAATCTCTTTCTCCTTCGCTCGTCTTTCCATCTTTTCCTTGTTATCTTTAACCACTTTTCATCACTTCCCTTCATTCATTTTCATTTCCACAGATAAGGGTATGTTCTCTCTCCCTCATTTGTTCTTCTGCAACTTCTGATTTGTTCATCTTCTTTTATCTGGGATCTCGTGGTACTACATGTATGTCTTGTTCACTCACTCATTTGGGTCTTTCGAGTTTTGTGTTTCATGCAGTTCTTATCGTTTCTATTCTGTTCCATTGATGTGGGTTTGGATTCTTTTAGACCAACTTTCACTTGTGCTCTATGAATTTGCTTTTGATGATGATGGGGGTGGGCTTGTTTTCTGTTGCATTCGTTATTTATCGATGGAACTTGAATTAATTGAATCCGATATTCAACGGTTTAAGTAGATTCATATCTTGATACCGATGAATGTTCGTTTGCTATAGTTTTCAAGCATGGATCAAATGATATAAGTTTTTAAGGTGTTAAGGCATCGGAATCGCATCTCCTGCTGAATTTGATGAATAAACCTTAATAAATAAACTGATAACTGTTTTATTTTGTTAATCAATGATTATCTCATACATCAATCGTAATTCTTTTTCTCGGGGATCGTGTACTACTATTTTTATTACTTTTTTGGTCTTGATGAGAATTATTGATTCATCCCTTTTCATCAAATATTTCAACAAATACTTGAGGGATCGTTTCAATTTGCTGGGTTTTGAATTAATTAATTTATCCGACTTTTCTTAATCACAGTTTTAAGTAGTCTGTTGAAAAGATGGAGGTTACTAATGTCACCGAGTATGAGGCTATTGCAAAGGAGAAGTTGCCAAAGATGGTTTATGACTACTATGCATCTGGTGCAGAGGACCAGTGGACCCTGAAGGAGAATCGAAATGCATTTTCCAGGATTTTGTACGTTCCTTTCCATGCTTTTGCCAGTGCTTTTCAATACAAACTTACTCCACTTTTTATCTTGTATTGTATATCTCTTTTTATTTGTAAGATAAATCTTTGAAAGAGAGTTTGAGGTGATATTTGGAAGTAGTACTTTTAGATAATACTTTTTTTCTATAATATTTTGGAAAAACACTTATATAGTTCTTTATCCAAGATTTTGAGTTATATGGACACGTTTGAATGGATAAAAATACCTTTAGTCTCAGTAGAAGCACTTTTCACAGTTTTCATGACAGTTAAAAATAGTTTTGAAAATTTTGAAGATTCTAAATATTGCTCTTGAAGCAAGCACTTAAGTAGTATTTTGTTCTCCATAATTATAAATTTAAAAAATAAATTTGACTTAAAACATCTCTGAAATTTTTTTAGAAACGTTTGTAGGGAGTTTTAGTTATGTTACTATACACCAAGAAATTCTTTTTCAATCTGAGTTTGTGTTCTTTGTTCTTCTTTTCTCTTCAGAGCAATATTAAAACTAATTATCTGATAGTTGCTAGCTTTCACTGAGACAGGTTCCGTCCCCGTATTCTTATTGATGTGAGTAAGATAGACATGTCAACAACTGTGTTGGGTTTCAAAATTTCAATGCCCATTATGATTGCTCCAACTGCCATGCAAAAGATGGCTCACCCTGAAGGTAAAAAACTTCCTAATTCCATTGTGAACTCCATTTTTCTAGTCAGTTATGCCGTGTCTGTCTATATTGGTTTAGAAAACTGTCAAATGCTTTGGATAGTTGGAACTTGTTGAAAGTTGATTTGTCAAGTTTAATGTATATGCACTACTTTTATAGCTTATAAATCCTTCTAGTCAACCACAGGCTTCTTTAGTTGCATTTCAAGATTAGTACTTACGTTAGCTTTGAATGAGAAAATTCAAACAGCAAGATGAAATTTATGCATTAGAGAAAATAACCATACTTATTTGTGCTCATTAGCACTTCAATTCCTACAAGAATGACACTGTATTGATCTGACTGATATTGTTGTTTCAATGCAGGGGAGTATGCAACAGCCAGAGCAGCGTCTGCAGCAGGCACAATTATGGTTTAAATTTCTTCTTCCAAGCCTAAAGTTTTTTTTATTCTCTCAAATATTTAGTACACATTCTTGACTTGAATTAGAAGTGTGGGATTGATTAAAGACTTTATTTCTATGCTTATTCAAGTAATTCTTGAATTGTTTCAGACTCTTTCCTCATGGGCTACTTCCAGTGTGGAAGAAGTTGCTTCCACTGGACCTGGCATTCGGTTCTTCCAGCTATATGTAAGAACAGTTTGTAAAGAATTTTCAAGAATAATAAAAAGCAATATTTTCTTCTTCGTTTGTCTCAAACATTTGGTCTTTACGCATTTCATTCGAATCCATTTTATTGCTTATTCAGGTGTACAAGGATAGGAATGTTGTGGCACAACTTGTGAGAAGAGCTGAAAAGGCTGGTTTCAAAGCCATTGCTCTGACTGTGGACACTCCAAGGCTTGGTCGCAGGGAAGCTGATATCAAGAACAGGTGAGTATGGATTTATTGAGTCAGCTAATACACGTTTGAGGTATATAATAAACCTTGTCATCAAATGTTTTTCACACATTGATGGCTCTTGATGCAGATTTACTTTGCCACCATTTTTGACATTGAAGAACTTTGAGGGATTGGATCTTGGTAAAATGGACAAGGTAAGACTGAAATGCCACTCTATGCTAAGTTCTGTTTCCATCAACAATGTCCATGATAACATTTTCTTTCTCTGTGGGGGTGTGTTTTTCTACAGGCAGACGACTCTGGACTGGCCTCATATGTTGCGGGGCAGATCGACCGTACTCTCAGTTGGCAGGTAACTAAAAAAGGTCATTCAAAGTTCAAAAGGACAATGTTATTGTTATTATTATTATTTTGAAACAAACAAGATAGCATCATGGAAGAAGCTTCTTTTTCCATTGACTTACAGGATGTGAAGTGGCTGCAGACCATCACCAAGTTGCCAATTCTAGTGAAGGGTGTGCTTACTGCTGAGGACAGTAAGTAAACTATGATCGAAGAGACTAAAACCACTAATGAAAAAAAGATGCTATAACAAGACATATTCATTTGATTCTATCAAGAACATTTTGTCATTTCCTTGGGATGATTTTAGTGTAGTTTGATGTTTGGCTTCCTAATTGCAGCAAGGATTGCCATTCAAAGTGGAGCTGCTGGAATAATTGTTTCAAATCATGGAGCTCGCCAACTTGACTATGTTCCTGCAACCATTGTGGCTTTGGAAGAGGTACTTGATACAATTGCACAATAGTAAATTTTGGTCGAAAAGATTATATTTTTCAAAGCAGATCGTCAGTTGGCCTAAAACAAAACATGTCTTTATCTTTTGAGCTATGATCACAATCTCATACTTGATGATTTCAGGTTGTTAAAGCTGCTAGAGGTGAAATTCCTGTTTTCCTGGATGGCGGTGTTCGACGTGGAACAGATGTTTTCAAAGCATTGGCACTTGGAGCATCTGGCATATTTGTAAGTAAAGAGAACACATATCGGACGATCACTTTGCTACTATCAACTATATGATCACTTTAACAAATATCATATTCATCAATGTTAGATTGGGAGGCCAGTGGTGTTCTCTTTGGCAGCGGAGGGCGAGGCCGGTGTGAGAAAAGTGCTTCAGATGTTGCGTGATGAGTTTGAGCTGACCATGGCATTGAGTGGCTGCCGCTCACTTCAGGAGATCACCCGCAGCCACATTGTGGCCGACTGGGACACTCCTCGCATCGTGCCGAGGCTATGAGATGTTGCATTTTGTATGCAGCAACAAACACCATTTGGCAGTCTTGAATTCTTGGGGTTGTAATTTATAGCATATGAACGATGTTCTGTTATGATAAAAATATGTAATGTCTTCTTGAATGGGATATTACATTCATCCTATATTGTTTCTGTTGCCAGCATTGATGATATTTTACTGAAGTTGACAACTGAATGTCTTTTTGGTTGCTGGTACAGGTGGT

mRNA sequence

CAAATTCGTCAACACCATTTTTTCCTTCATCCCAAGCAATTCGAATGGAATTTAGATTACATTATTGAGGATTGAGAATGAGCGACAGTCTTGCGAACGCCCTCCACACAAGTTTTTATTTCTGATTAATATGGGAAATTTCTGTGAAACAGATTTGGTTTCTAACGCTCACCTTCAAAATCCAATCCAAATAATGTGGTCTCTCTCTCTTTTCCCCAATTTGATCCCACCCCTTTTGTCTTTACCTTCAATCCGATCACGGTCTTCCTCTATCGCCCTTATGCTATATTTGTTGTTTGTTTTCGCATTCCCCATTTCGAGAGCTCAATCTCTTTCTCCTTCGCTCGTCTTTCCATCTTTTCCTTGTTATCTTTAACCACTTTTCATCACTTCCCTTCATTCATTTTCATTTCCACAGATAAGGGTATGTTCTCTCTCCCTCATTTGTTCTTCTGCAACTTCTGATTTGTTCATCTTCTTTTATCTGGGATCTCGTGGTACTACATTTTTAAGTAGTCTGTTGAAAAGATGGAGGTTACTAATGTCACCGAGTATGAGGCTATTGCAAAGGAGAAGTTGCCAAAGATGGTTTATGACTACTATGCATCTGGTGCAGAGGACCAGTGGACCCTGAAGGAGAATCGAAATGCATTTTCCAGGATTTTGTTCCGTCCCCGTATTCTTATTGATGTGAGTAAGATAGACATGTCAACAACTGTGTTGGGTTTCAAAATTTCAATGCCCATTATGATTGCTCCAACTGCCATGCAAAAGATGGCTCACCCTGAAGGGGAGTATGCAACAGCCAGAGCAGCGTCTGCAGCAGGCACAATTATGACTCTTTCCTCATGGGCTACTTCCAGTGTGGAAGAAGTTGCTTCCACTGGACCTGGCATTCGGTTCTTCCAGCTATATGTGTACAAGGATAGGAATGTTGTGGCACAACTTGTGAGAAGAGCTGAAAAGGCTGGTTTCAAAGCCATTGCTCTGACTGTGGACACTCCAAGGCTTGGTCGCAGGGAAGCTGATATCAAGAACAGATTTACTTTGCCACCATTTTTGACATTGAAGAACTTTGAGGGATTGGATCTTGGTAAAATGGACAAGGTAAGACTGAAATGCCACTCTATGCTAAGTTCTGTTTCCATCAACAATGTCCATGATAACATTTTCTTTCTCTGTGGGGGTGTGTTTTTCTACAGGCAGACGACTCTGGACTGGCCTCATATGTTGCGGGGCAGATCGACCGTACTCTCAGTTGGCAGCATCATGGAAGAAGCTTCTTTTTCCATTGACTTACAGGATGTGAAGTGGCTGCAGACCATCACCAAGTTGCCAATTCTAGTGAAGGGTGTGCTTACTGCTGAGGACACAAGGATTGCCATTCAAAGTGGAGCTGCTGGAATAATTGTTTCAAATCATGGAGCTCGCCAACTTGACTATGTTCCTGCAACCATTGTGGCTTTGGAAGAGGTTGTTAAAGCTGCTAGAGGTGAAATTCCTGTTTTCCTGGATGGCGGTGTTCGACGTGGAACAGATGTTTTCAAAGCATTGGCACTTGGAGCATCTGGCATATTTATTGGGAGGCCAGTGGTGTTCTCTTTGGCAGCGGAGGGCGAGGCCGGTGTGAGAAAAGTGCTTCAGATGTTGCGTGATGAGTTTGAGCTGACCATGGCATTGAGTGGCTGCCGCTCACTTCAGGAGATCACCCGCAGCCACATTGTGGCCGACTGGGACACTCCTCGCATCGTGCCGAGGCTATGAGATGTTGCATTTTGTATGCAGCAACAAACACCATTTGGCAGTCTTGAATTCTTGGGGTTGTAATTTATAGCATATGAACGATGTTCTGTTATGATAAAAATATGTAATGTCTTCTTGAATGGGATATTACATTCATCCTATATTGTTTCTGTTGCCAGCATTGATGATATTTTACTGAAGTTGACAACTGAATGTCTTTTTGGTTGCTGGTACAGGTGGT

Coding sequence (CDS)

ATGGAGGTTACTAATGTCACCGAGTATGAGGCTATTGCAAAGGAGAAGTTGCCAAAGATGGTTTATGACTACTATGCATCTGGTGCAGAGGACCAGTGGACCCTGAAGGAGAATCGAAATGCATTTTCCAGGATTTTGTTCCGTCCCCGTATTCTTATTGATGTGAGTAAGATAGACATGTCAACAACTGTGTTGGGTTTCAAAATTTCAATGCCCATTATGATTGCTCCAACTGCCATGCAAAAGATGGCTCACCCTGAAGGGGAGTATGCAACAGCCAGAGCAGCGTCTGCAGCAGGCACAATTATGACTCTTTCCTCATGGGCTACTTCCAGTGTGGAAGAAGTTGCTTCCACTGGACCTGGCATTCGGTTCTTCCAGCTATATGTGTACAAGGATAGGAATGTTGTGGCACAACTTGTGAGAAGAGCTGAAAAGGCTGGTTTCAAAGCCATTGCTCTGACTGTGGACACTCCAAGGCTTGGTCGCAGGGAAGCTGATATCAAGAACAGATTTACTTTGCCACCATTTTTGACATTGAAGAACTTTGAGGGATTGGATCTTGGTAAAATGGACAAGGTAAGACTGAAATGCCACTCTATGCTAAGTTCTGTTTCCATCAACAATGTCCATGATAACATTTTCTTTCTCTGTGGGGGTGTGTTTTTCTACAGGCAGACGACTCTGGACTGGCCTCATATGTTGCGGGGCAGATCGACCGTACTCTCAGTTGGCAGCATCATGGAAGAAGCTTCTTTTTCCATTGACTTACAGGATGTGAAGTGGCTGCAGACCATCACCAAGTTGCCAATTCTAGTGAAGGGTGTGCTTACTGCTGAGGACACAAGGATTGCCATTCAAAGTGGAGCTGCTGGAATAATTGTTTCAAATCATGGAGCTCGCCAACTTGACTATGTTCCTGCAACCATTGTGGCTTTGGAAGAGGTTGTTAAAGCTGCTAGAGGTGAAATTCCTGTTTTCCTGGATGGCGGTGTTCGACGTGGAACAGATGTTTTCAAAGCATTGGCACTTGGAGCATCTGGCATATTTATTGGGAGGCCAGTGGTGTTCTCTTTGGCAGCGGAGGGCGAGGCCGGTGTGAGAAAAGTGCTTCAGATGTTGCGTGATGAGTTTGAGCTGACCATGGCATTGAGTGGCTGCCGCTCACTTCAGGAGATCACCCGCAGCCACATTGTGGCCGACTGGGACACTCCTCGCATCGTGCCGAGGCTATGA

Protein sequence

MEVTNVTEYEAIAKEKLPKMVYDYYASGAEDQWTLKENRNAFSRILFRPRILIDVSKIDMSTTVLGFKISMPIMIAPTAMQKMAHPEGEYATARAASAAGTIMTLSSWATSSVEEVASTGPGIRFFQLYVYKDRNVVAQLVRRAEKAGFKAIALTVDTPRLGRREADIKNRFTLPPFLTLKNFEGLDLGKMDKVRLKCHSMLSSVSINNVHDNIFFLCGGVFFYRQTTLDWPHMLRGRSTVLSVGSIMEEASFSIDLQDVKWLQTITKLPILVKGVLTAEDTRIAIQSGAAGIIVSNHGARQLDYVPATIVALEEVVKAARGEIPVFLDGGVRRGTDVFKALALGASGIFIGRPVVFSLAAEGEAGVRKVLQMLRDEFELTMALSGCRSLQEITRSHIVADWDTPRIVPRL
Homology
BLAST of Cla97C01G024660 vs. NCBI nr
Match: XP_008439620.1 (PREDICTED: peroxisomal (S)-2-hydroxy-acid oxidase GLO1 [Cucumis melo] >XP_008439621.1 PREDICTED: peroxisomal (S)-2-hydroxy-acid oxidase GLO1 [Cucumis melo] >TYK13291.1 peroxisomal (S)-2-hydroxy-acid oxidase GLO1 [Cucumis melo var. makuwa])

HSP 1 Score: 649.4 bits (1674), Expect = 2.0e-182
Identity = 351/411 (85.40%), Postives = 358/411 (87.10%), Query Frame = 0

Query: 1   MEVTNVTEYEAIAKEKLPKMVYDYYASGAEDQWTLKENRNAFSRILFRPRILIDVSKIDM 60
           ME+TNVTEYEAIAKEKLPKMVYDYYASGAEDQWTLKENRNAFSRILFRPRILIDVSKIDM
Sbjct: 1   MEITNVTEYEAIAKEKLPKMVYDYYASGAEDQWTLKENRNAFSRILFRPRILIDVSKIDM 60

Query: 61  STTVLGFKISMPIMIAPTAMQKMAHPEGEYATARAASAAGTIMTLSSWATSSVEEVASTG 120
           STTVLGFKISMPIMIAPTAMQKMAHPEGEYATARAASAAGTIMTLSSWATSSVEEVASTG
Sbjct: 61  STTVLGFKISMPIMIAPTAMQKMAHPEGEYATARAASAAGTIMTLSSWATSSVEEVASTG 120

Query: 121 PGIRFFQLYVYKDRNVVAQLVRRAEKAGFKAIALTVDTPRLGRREADIKNRFTLPPFLTL 180
           PGIRFFQLYVYKDRNVVAQLVRRAEKAGFKAIALTVDTPRLGRREADIKNRFTLPPFLTL
Sbjct: 121 PGIRFFQLYVYKDRNVVAQLVRRAEKAGFKAIALTVDTPRLGRREADIKNRFTLPPFLTL 180

Query: 181 KNFEGLDLGKMDKVRLKCHSMLSSVSINNVHDNIFFLCGGVFFYRQTTLDWPHMLRGRST 240
           KNFEGLDLGKMDK      S L+S           ++ G +      TL W         
Sbjct: 181 KNFEGLDLGKMDKAD---DSGLAS-----------YVAGQI----DRTLSW--------- 240

Query: 241 VLSVGSIMEEASFSIDLQDVKWLQTITKLPILVKGVLTAEDTRIAIQSGAAGIIVSNHGA 300
                            QDVKWLQTITKLPILVKGVLTAEDTRIAIQSGAAGIIVSNHGA
Sbjct: 241 -----------------QDVKWLQTITKLPILVKGVLTAEDTRIAIQSGAAGIIVSNHGA 300

Query: 301 RQLDYVPATIVALEEVVKAARGEIPVFLDGGVRRGTDVFKALALGASGIFIGRPVVFSLA 360
           RQLDYVPATIVALEEVVKAARGE+PVFLDGGVRRGTDVFKALALGASGIFIGRPVVFSLA
Sbjct: 301 RQLDYVPATIVALEEVVKAARGEVPVFLDGGVRRGTDVFKALALGASGIFIGRPVVFSLA 360

Query: 361 AEGEAGVRKVLQMLRDEFELTMALSGCRSLQEITRSHIVADWDTPRIVPRL 412
           AEGEAGVRKVLQMLRDEFELTMALSGCRSLQEITRSHI+ADWDTPRIVPRL
Sbjct: 361 AEGEAGVRKVLQMLRDEFELTMALSGCRSLQEITRSHILADWDTPRIVPRL 367

BLAST of Cla97C01G024660 vs. NCBI nr
Match: XP_038883246.1 ((S)-2-hydroxy-acid oxidase GLO1 [Benincasa hispida])

HSP 1 Score: 648.7 bits (1672), Expect = 3.5e-182
Identity = 349/411 (84.91%), Postives = 358/411 (87.10%), Query Frame = 0

Query: 1   MEVTNVTEYEAIAKEKLPKMVYDYYASGAEDQWTLKENRNAFSRILFRPRILIDVSKIDM 60
           ME+TNVTEYEAIAKEKLPKMVYDYYASGAEDQWTLKENRNAFSRILFRPRILIDVSKIDM
Sbjct: 1   MEITNVTEYEAIAKEKLPKMVYDYYASGAEDQWTLKENRNAFSRILFRPRILIDVSKIDM 60

Query: 61  STTVLGFKISMPIMIAPTAMQKMAHPEGEYATARAASAAGTIMTLSSWATSSVEEVASTG 120
           STTVLGFKISMPIMIAPTAMQKMAHPEGEYATARAASAAGTIMTLSSWATSSVEEVASTG
Sbjct: 61  STTVLGFKISMPIMIAPTAMQKMAHPEGEYATARAASAAGTIMTLSSWATSSVEEVASTG 120

Query: 121 PGIRFFQLYVYKDRNVVAQLVRRAEKAGFKAIALTVDTPRLGRREADIKNRFTLPPFLTL 180
           PGIRFFQLYVYKDRNVVAQLVRRAE+AGFKAIALTVDTPRLGRREADIKNRFTLPPFLTL
Sbjct: 121 PGIRFFQLYVYKDRNVVAQLVRRAERAGFKAIALTVDTPRLGRREADIKNRFTLPPFLTL 180

Query: 181 KNFEGLDLGKMDKVRLKCHSMLSSVSINNVHDNIFFLCGGVFFYRQTTLDWPHMLRGRST 240
           KNFEGLDLGKMDK      S L+S           ++ G +      TL W         
Sbjct: 181 KNFEGLDLGKMDKAD---DSGLAS-----------YVAGQI----DRTLSW--------- 240

Query: 241 VLSVGSIMEEASFSIDLQDVKWLQTITKLPILVKGVLTAEDTRIAIQSGAAGIIVSNHGA 300
                            QDVKWLQTITKLPILVKGVLTAEDTRIAIQSGAAG+IVSNHGA
Sbjct: 241 -----------------QDVKWLQTITKLPILVKGVLTAEDTRIAIQSGAAGVIVSNHGA 300

Query: 301 RQLDYVPATIVALEEVVKAARGEIPVFLDGGVRRGTDVFKALALGASGIFIGRPVVFSLA 360
           RQLDYVPATI+ALEEVVKAARGE+PVFLDGGVRRGTDVFKALALGASGIFIGRPVVFSLA
Sbjct: 301 RQLDYVPATIIALEEVVKAARGEVPVFLDGGVRRGTDVFKALALGASGIFIGRPVVFSLA 360

Query: 361 AEGEAGVRKVLQMLRDEFELTMALSGCRSLQEITRSHIVADWDTPRIVPRL 412
           AEGEAGVRKVLQMLRDEFELTMALSGCRSLQEITRSHIVADWDTPRIVPRL
Sbjct: 361 AEGEAGVRKVLQMLRDEFELTMALSGCRSLQEITRSHIVADWDTPRIVPRL 367

BLAST of Cla97C01G024660 vs. NCBI nr
Match: KAA0052535.1 (peroxisomal (S)-2-hydroxy-acid oxidase GLO1 [Cucumis melo var. makuwa])

HSP 1 Score: 646.7 bits (1667), Expect = 1.3e-181
Identity = 349/411 (84.91%), Postives = 358/411 (87.10%), Query Frame = 0

Query: 1   MEVTNVTEYEAIAKEKLPKMVYDYYASGAEDQWTLKENRNAFSRILFRPRILIDVSKIDM 60
           ME+TNVTEYEAIAKEKLPKMVYDYYASGAEDQWTLKENRNAFSRILFRPRILIDVSKIDM
Sbjct: 1   MEITNVTEYEAIAKEKLPKMVYDYYASGAEDQWTLKENRNAFSRILFRPRILIDVSKIDM 60

Query: 61  STTVLGFKISMPIMIAPTAMQKMAHPEGEYATARAASAAGTIMTLSSWATSSVEEVASTG 120
           STTVLGFKISMPIMIAPTAMQKMAHPEGEYATARAASAAGTIMTLSSWATSSVEEVASTG
Sbjct: 61  STTVLGFKISMPIMIAPTAMQKMAHPEGEYATARAASAAGTIMTLSSWATSSVEEVASTG 120

Query: 121 PGIRFFQLYVYKDRNVVAQLVRRAEKAGFKAIALTVDTPRLGRREADIKNRFTLPPFLTL 180
           PGIRFFQLYVYKDRNVVAQLVRRAEKAGFKAIALTVDTPRLGRREADIKNRFTLPPFLTL
Sbjct: 121 PGIRFFQLYVYKDRNVVAQLVRRAEKAGFKAIALTVDTPRLGRREADIKNRFTLPPFLTL 180

Query: 181 KNFEGLDLGKMDKVRLKCHSMLSSVSINNVHDNIFFLCGGVFFYRQTTLDWPHMLRGRST 240
           KNFEGLDLGKMDK      S L+S           ++ G +      TL W         
Sbjct: 181 KNFEGLDLGKMDKAD---DSGLAS-----------YVAGQI----DRTLSW--------- 240

Query: 241 VLSVGSIMEEASFSIDLQDVKWLQTITKLPILVKGVLTAEDTRIAIQSGAAGIIVSNHGA 300
                            QDVKWLQTITKLPILVKGVLTAED++IAIQSGAAGIIVSNHGA
Sbjct: 241 -----------------QDVKWLQTITKLPILVKGVLTAEDSKIAIQSGAAGIIVSNHGA 300

Query: 301 RQLDYVPATIVALEEVVKAARGEIPVFLDGGVRRGTDVFKALALGASGIFIGRPVVFSLA 360
           RQLDYVPATIVALEEVVKAARGE+PVFLDGGVRRGTDVFKALALGASGIFIGRPVVFSLA
Sbjct: 301 RQLDYVPATIVALEEVVKAARGEVPVFLDGGVRRGTDVFKALALGASGIFIGRPVVFSLA 360

Query: 361 AEGEAGVRKVLQMLRDEFELTMALSGCRSLQEITRSHIVADWDTPRIVPRL 412
           AEGEAGVRKVLQMLRDEFELTMALSGCRSLQEITRSHI+ADWDTPRIVPRL
Sbjct: 361 AEGEAGVRKVLQMLRDEFELTMALSGCRSLQEITRSHILADWDTPRIVPRL 367

BLAST of Cla97C01G024660 vs. NCBI nr
Match: XP_022926037.1 ((S)-2-hydroxy-acid oxidase GLO1 [Cucurbita moschata] >XP_022926038.1 (S)-2-hydroxy-acid oxidase GLO1 [Cucurbita moschata] >XP_022978357.1 (S)-2-hydroxy-acid oxidase GLO1 [Cucurbita maxima] >XP_022978358.1 (S)-2-hydroxy-acid oxidase GLO1 [Cucurbita maxima] >XP_023544737.1 (S)-2-hydroxy-acid oxidase GLO1 [Cucurbita pepo subsp. pepo])

HSP 1 Score: 646.0 bits (1665), Expect = 2.2e-181
Identity = 347/411 (84.43%), Postives = 358/411 (87.10%), Query Frame = 0

Query: 1   MEVTNVTEYEAIAKEKLPKMVYDYYASGAEDQWTLKENRNAFSRILFRPRILIDVSKIDM 60
           ME+TNVTEYEAIAKEKLPKMVYDYYASGAEDQWTLKENRNAFSRILFRPRILIDVSKIDM
Sbjct: 1   MEITNVTEYEAIAKEKLPKMVYDYYASGAEDQWTLKENRNAFSRILFRPRILIDVSKIDM 60

Query: 61  STTVLGFKISMPIMIAPTAMQKMAHPEGEYATARAASAAGTIMTLSSWATSSVEEVASTG 120
           STTVLGFKISMPIMIAPTAMQKMAHPEGEYATARAASAAGTIMTLSSWATSSVEEVASTG
Sbjct: 61  STTVLGFKISMPIMIAPTAMQKMAHPEGEYATARAASAAGTIMTLSSWATSSVEEVASTG 120

Query: 121 PGIRFFQLYVYKDRNVVAQLVRRAEKAGFKAIALTVDTPRLGRREADIKNRFTLPPFLTL 180
           PGIRFFQLYVYKDRNVVAQLVRRAEKAGFKAIALTVDTPRLGRREADIKNRFTLPPFLTL
Sbjct: 121 PGIRFFQLYVYKDRNVVAQLVRRAEKAGFKAIALTVDTPRLGRREADIKNRFTLPPFLTL 180

Query: 181 KNFEGLDLGKMDKVRLKCHSMLSSVSINNVHDNIFFLCGGVFFYRQTTLDWPHMLRGRST 240
           KNFEGLDLGKMD+      S L+S           ++ G +      TL W         
Sbjct: 181 KNFEGLDLGKMDQAD---DSGLAS-----------YVAGQI----DRTLSW--------- 240

Query: 241 VLSVGSIMEEASFSIDLQDVKWLQTITKLPILVKGVLTAEDTRIAIQSGAAGIIVSNHGA 300
                            QDVKWLQTITKLPILVKGVLTAEDTRIA+QSGAAGIIVSNHGA
Sbjct: 241 -----------------QDVKWLQTITKLPILVKGVLTAEDTRIAVQSGAAGIIVSNHGA 300

Query: 301 RQLDYVPATIVALEEVVKAARGEIPVFLDGGVRRGTDVFKALALGASGIFIGRPVVFSLA 360
           RQLDYVPATI+ALEEVVKAARGE+PVFLDGGVRRGTDVFKALALGASGIFIGRPVVFSLA
Sbjct: 301 RQLDYVPATIMALEEVVKAARGEVPVFLDGGVRRGTDVFKALALGASGIFIGRPVVFSLA 360

Query: 361 AEGEAGVRKVLQMLRDEFELTMALSGCRSLQEITRSHIVADWDTPRIVPRL 412
           AEGEAGVRKVLQMLRDEFELTMALSGCRSLQEITR+HIVADWDTPR+VPRL
Sbjct: 361 AEGEAGVRKVLQMLRDEFELTMALSGCRSLQEITRNHIVADWDTPRVVPRL 367

BLAST of Cla97C01G024660 vs. NCBI nr
Match: KAG7034728.1 (Peroxisomal (S)-2-hydroxy-acid oxidase GLO1 [Cucurbita argyrosperma subsp. argyrosperma])

HSP 1 Score: 646.0 bits (1665), Expect = 2.2e-181
Identity = 347/411 (84.43%), Postives = 358/411 (87.10%), Query Frame = 0

Query: 1   MEVTNVTEYEAIAKEKLPKMVYDYYASGAEDQWTLKENRNAFSRILFRPRILIDVSKIDM 60
           ME+TNVTEYEAIAKEKLPKMVYDYYASGAEDQWTLKENRNAFSRILFRPRILIDVSKIDM
Sbjct: 11  MEITNVTEYEAIAKEKLPKMVYDYYASGAEDQWTLKENRNAFSRILFRPRILIDVSKIDM 70

Query: 61  STTVLGFKISMPIMIAPTAMQKMAHPEGEYATARAASAAGTIMTLSSWATSSVEEVASTG 120
           STTVLGFKISMPIMIAPTAMQKMAHPEGEYATARAASAAGTIMTLSSWATSSVEEVASTG
Sbjct: 71  STTVLGFKISMPIMIAPTAMQKMAHPEGEYATARAASAAGTIMTLSSWATSSVEEVASTG 130

Query: 121 PGIRFFQLYVYKDRNVVAQLVRRAEKAGFKAIALTVDTPRLGRREADIKNRFTLPPFLTL 180
           PGIRFFQLYVYKDRNVVAQLVRRAEKAGFKAIALTVDTPRLGRREADIKNRFTLPPFLTL
Sbjct: 131 PGIRFFQLYVYKDRNVVAQLVRRAEKAGFKAIALTVDTPRLGRREADIKNRFTLPPFLTL 190

Query: 181 KNFEGLDLGKMDKVRLKCHSMLSSVSINNVHDNIFFLCGGVFFYRQTTLDWPHMLRGRST 240
           KNFEGLDLGKMD+      S L+S           ++ G +      TL W         
Sbjct: 191 KNFEGLDLGKMDQAD---DSGLAS-----------YVAGQI----DRTLSW--------- 250

Query: 241 VLSVGSIMEEASFSIDLQDVKWLQTITKLPILVKGVLTAEDTRIAIQSGAAGIIVSNHGA 300
                            QDVKWLQTITKLPILVKGVLTAEDTRIA+QSGAAGIIVSNHGA
Sbjct: 251 -----------------QDVKWLQTITKLPILVKGVLTAEDTRIAVQSGAAGIIVSNHGA 310

Query: 301 RQLDYVPATIVALEEVVKAARGEIPVFLDGGVRRGTDVFKALALGASGIFIGRPVVFSLA 360
           RQLDYVPATI+ALEEVVKAARGE+PVFLDGGVRRGTDVFKALALGASGIFIGRPVVFSLA
Sbjct: 311 RQLDYVPATIMALEEVVKAARGEVPVFLDGGVRRGTDVFKALALGASGIFIGRPVVFSLA 370

Query: 361 AEGEAGVRKVLQMLRDEFELTMALSGCRSLQEITRSHIVADWDTPRIVPRL 412
           AEGEAGVRKVLQMLRDEFELTMALSGCRSLQEITR+HIVADWDTPR+VPRL
Sbjct: 371 AEGEAGVRKVLQMLRDEFELTMALSGCRSLQEITRNHIVADWDTPRVVPRL 377

BLAST of Cla97C01G024660 vs. ExPASy Swiss-Prot
Match: Q9LRR9 ((S)-2-hydroxy-acid oxidase GLO1 OS=Arabidopsis thaliana OX=3702 GN=GLO1 PE=1 SV=1)

HSP 1 Score: 600.9 bits (1548), Expect = 1.1e-170
Identity = 322/411 (78.35%), Postives = 346/411 (84.18%), Query Frame = 0

Query: 1   MEVTNVTEYEAIAKEKLPKMVYDYYASGAEDQWTLKENRNAFSRILFRPRILIDVSKIDM 60
           ME+TNVTEY+AIAK+KLPKMVYDYYASGAEDQWTL+ENRNAF+RILFRPRILIDVSKIDM
Sbjct: 1   MEITNVTEYDAIAKQKLPKMVYDYYASGAEDQWTLQENRNAFARILFRPRILIDVSKIDM 60

Query: 61  STTVLGFKISMPIMIAPTAMQKMAHPEGEYATARAASAAGTIMTLSSWATSSVEEVASTG 120
           +TTVLGFKISMPIM+APTAMQKMAHP+GEYATARAASAAGTIMTLSSWATSSVEEVASTG
Sbjct: 61  TTTVLGFKISMPIMVAPTAMQKMAHPDGEYATARAASAAGTIMTLSSWATSSVEEVASTG 120

Query: 121 PGIRFFQLYVYKDRNVVAQLVRRAEKAGFKAIALTVDTPRLGRREADIKNRFTLPPFLTL 180
           PGIRFFQLYVYK+RNVV QLVRRAE+AGFKAIALTVDTPRLGRRE+DIKNRFTLPP LTL
Sbjct: 121 PGIRFFQLYVYKNRNVVEQLVRRAERAGFKAIALTVDTPRLGRRESDIKNRFTLPPNLTL 180

Query: 181 KNFEGLDLGKMDKVRLKCHSMLSSVSINNVHDNIFFLCGGVFFYRQTTLDWPHMLRGRST 240
           KNFEGLDLGKMD+      S L+S           ++ G +      TL W         
Sbjct: 181 KNFEGLDLGKMDEAN---DSGLAS-----------YVAGQI----DRTLSW--------- 240

Query: 241 VLSVGSIMEEASFSIDLQDVKWLQTITKLPILVKGVLTAEDTRIAIQSGAAGIIVSNHGA 300
                            +DV+WLQTITKLPILVKGVLT ED RIAIQ+GAAGIIVSNHGA
Sbjct: 241 -----------------KDVQWLQTITKLPILVKGVLTGEDARIAIQAGAAGIIVSNHGA 300

Query: 301 RQLDYVPATIVALEEVVKAARGEIPVFLDGGVRRGTDVFKALALGASGIFIGRPVVFSLA 360
           RQLDYVPATI ALEEVVKA +G IPVFLDGGVRRGTDVFKALALGASGIFIGRPVVFSLA
Sbjct: 301 RQLDYVPATISALEEVVKATQGRIPVFLDGGVRRGTDVFKALALGASGIFIGRPVVFSLA 360

Query: 361 AEGEAGVRKVLQMLRDEFELTMALSGCRSLQEITRSHIVADWDTPRIVPRL 412
           AEGEAGVRKVLQMLRDEFELTMALSGCRSL+EI+R+HI  +WDTPR   RL
Sbjct: 361 AEGEAGVRKVLQMLRDEFELTMALSGCRSLKEISRNHITTEWDTPRPSARL 367

BLAST of Cla97C01G024660 vs. ExPASy Swiss-Prot
Match: P05414 (Peroxisomal (S)-2-hydroxy-acid oxidase OS=Spinacia oleracea OX=3562 PE=1 SV=1)

HSP 1 Score: 600.5 bits (1547), Expect = 1.4e-170
Identity = 322/413 (77.97%), Postives = 345/413 (83.54%), Query Frame = 0

Query: 1   MEVTNVTEYEAIAKEKLPKMVYDYYASGAEDQWTLKENRNAFSRILFRPRILIDVSKIDM 60
           ME+TNV EYEAIAK+KLPKMVYDYYASGAEDQWTL ENRNAFSRILFRPRILIDV+ IDM
Sbjct: 1   MEITNVNEYEAIAKQKLPKMVYDYYASGAEDQWTLAENRNAFSRILFRPRILIDVTNIDM 60

Query: 61  STTVLGFKISMPIMIAPTAMQKMAHPEGEYATARAASAAGTIMTLSSWATSSVEEVASTG 120
           +TT+LGFKISMPIMIAPTAMQKMAHPEGEYATARAASAAGTIMTLSSWATSSVEEVASTG
Sbjct: 61  TTTILGFKISMPIMIAPTAMQKMAHPEGEYATARAASAAGTIMTLSSWATSSVEEVASTG 120

Query: 121 PGIRFFQLYVYKDRNVVAQLVRRAEKAGFKAIALTVDTPRLGRREADIKNRFTLPPFLTL 180
           PGIRFFQLYVYKDRNVVAQLVRRAE+AGFKAIALTVDTPRLGRREADIKNRF LPPFLTL
Sbjct: 121 PGIRFFQLYVYKDRNVVAQLVRRAERAGFKAIALTVDTPRLGRREADIKNRFVLPPFLTL 180

Query: 181 KNFEGLDLGKMDKVRLKCHSMLSSVSINNVHDNIFFLCGGVFFYRQTTLDWPHMLRGRST 240
           KNFEG+DLGKMDK      S LSS           ++ G +      +L W         
Sbjct: 181 KNFEGIDLGKMDKAN---DSGLSS-----------YVAGQI----DRSLSW--------- 240

Query: 241 VLSVGSIMEEASFSIDLQDVKWLQTITKLPILVKGVLTAEDTRIAIQSGAAGIIVSNHGA 300
                            +DV WLQTIT LPILVKGV+TAED R+A+Q GAAGIIVSNHGA
Sbjct: 241 -----------------KDVAWLQTITSLPILVKGVITAEDARLAVQHGAAGIIVSNHGA 300

Query: 301 RQLDYVPATIVALEEVVKAARGEIPVFLDGGVRRGTDVFKALALGASGIFIGRPVVFSLA 360
           RQLDYVPATI+ALEEVVKAA+G IPVFLDGGVRRGTDVFKALALGA+G+FIGRPVVFSLA
Sbjct: 301 RQLDYVPATIMALEEVVKAAQGRIPVFLDGGVRRGTDVFKALALGAAGVFIGRPVVFSLA 360

Query: 361 AEGEAGVRKVLQMLRDEFELTMALSGCRSLQEITRSHIVADWDTP--RIVPRL 412
           AEGEAGV+KVLQM+RDEFELTMALSGCRSL+EI+RSHI ADWD P  R V RL
Sbjct: 361 AEGEAGVKKVLQMMRDEFELTMALSGCRSLKEISRSHIAADWDGPSSRAVARL 369

BLAST of Cla97C01G024660 vs. ExPASy Swiss-Prot
Match: Q9LRS0 ((S)-2-hydroxy-acid oxidase GLO2 OS=Arabidopsis thaliana OX=3702 GN=GLO2 PE=1 SV=1)

HSP 1 Score: 596.7 bits (1537), Expect = 2.0e-169
Identity = 317/411 (77.13%), Postives = 344/411 (83.70%), Query Frame = 0

Query: 1   MEVTNVTEYEAIAKEKLPKMVYDYYASGAEDQWTLKENRNAFSRILFRPRILIDVSKIDM 60
           ME+TNVTEY+AIAK KLPKMVYDYYASGAEDQWTL+ENRNAF+RILFRPRILIDV+KIDM
Sbjct: 1   MEITNVTEYDAIAKAKLPKMVYDYYASGAEDQWTLQENRNAFARILFRPRILIDVNKIDM 60

Query: 61  STTVLGFKISMPIMIAPTAMQKMAHPEGEYATARAASAAGTIMTLSSWATSSVEEVASTG 120
           +TTVLGFKISMPIM+APTA QKMAHP+GEYATARAASAAGTIMTLSSWATSSVEEVASTG
Sbjct: 61  ATTVLGFKISMPIMVAPTAFQKMAHPDGEYATARAASAAGTIMTLSSWATSSVEEVASTG 120

Query: 121 PGIRFFQLYVYKDRNVVAQLVRRAEKAGFKAIALTVDTPRLGRREADIKNRFTLPPFLTL 180
           PGIRFFQLYVYK+R VV QLVRRAEKAGFKAIALTVDTPRLGRRE+DIKNRFTLPP LTL
Sbjct: 121 PGIRFFQLYVYKNRKVVEQLVRRAEKAGFKAIALTVDTPRLGRRESDIKNRFTLPPNLTL 180

Query: 181 KNFEGLDLGKMDKVRLKCHSMLSSVSINNVHDNIFFLCGGVFFYRQTTLDWPHMLRGRST 240
           KNFEGLDLGKMD+      S L+S           ++ G +      TL W         
Sbjct: 181 KNFEGLDLGKMDEAN---DSGLAS-----------YVAGQI----DRTLSW--------- 240

Query: 241 VLSVGSIMEEASFSIDLQDVKWLQTITKLPILVKGVLTAEDTRIAIQSGAAGIIVSNHGA 300
                            +D++WLQTIT +PILVKGVLT ED RIAIQ+GAAGIIVSNHGA
Sbjct: 241 -----------------KDIQWLQTITNMPILVKGVLTGEDARIAIQAGAAGIIVSNHGA 300

Query: 301 RQLDYVPATIVALEEVVKAARGEIPVFLDGGVRRGTDVFKALALGASGIFIGRPVVFSLA 360
           RQLDYVPATI ALEEVVKA +G +PVFLDGGVRRGTDVFKALALGASGIFIGRPVVF+LA
Sbjct: 301 RQLDYVPATISALEEVVKATQGRVPVFLDGGVRRGTDVFKALALGASGIFIGRPVVFALA 360

Query: 361 AEGEAGVRKVLQMLRDEFELTMALSGCRSLQEITRSHIVADWDTPRIVPRL 412
           AEGEAGV+KVLQMLRDEFELTMALSGCRSL EITR+HIV +WDTPR +PRL
Sbjct: 361 AEGEAGVKKVLQMLRDEFELTMALSGCRSLSEITRNHIVTEWDTPRHLPRL 367

BLAST of Cla97C01G024660 vs. ExPASy Swiss-Prot
Match: B8B7C5 (Peroxisomal (S)-2-hydroxy-acid oxidase GLO5 OS=Oryza sativa subsp. indica OX=39946 GN=GLO5 PE=3 SV=1)

HSP 1 Score: 578.2 bits (1489), Expect = 7.5e-164
Identity = 306/408 (75.00%), Postives = 340/408 (83.33%), Query Frame = 0

Query: 2   EVTNVTEYEAIAKEKLPKMVYDYYASGAEDQWTLKENRNAFSRILFRPRILIDVSKIDMS 61
           E+TNVTEY+AIAK+KLPKM+YDYYASGAED+WTL+ENR AF+RILFRPRILIDVSKIDM+
Sbjct: 3   EITNVTEYQAIAKQKLPKMIYDYYASGAEDEWTLQENREAFARILFRPRILIDVSKIDMA 62

Query: 62  TTVLGFKISMPIMIAPTAMQKMAHPEGEYATARAASAAGTIMTLSSWATSSVEEVASTGP 121
           TTVLGFKISMPIMIAP+AMQKMAHP+GEYATARAASAAGTIMTLSSWATSSVEEVASTGP
Sbjct: 63  TTVLGFKISMPIMIAPSAMQKMAHPDGEYATARAASAAGTIMTLSSWATSSVEEVASTGP 122

Query: 122 GIRFFQLYVYKDRNVVAQLVRRAEKAGFKAIALTVDTPRLGRREADIKNRFTLPPFLTLK 181
           GIRFFQLYVYKDR VV QLVRRAE+AGFKAIALTVDTPRLGRREADIKNRF LPPFLTLK
Sbjct: 123 GIRFFQLYVYKDRRVVEQLVRRAERAGFKAIALTVDTPRLGRREADIKNRFVLPPFLTLK 182

Query: 182 NFEGLDLGKMDKVRLKCHSMLSSVSINNVHDNIFFLCGGVFFYRQTTLDWPHMLRGRSTV 241
           NFEGL+LGKMD+      S L+S           ++ G +      TL W          
Sbjct: 183 NFEGLELGKMDQA---SDSGLAS-----------YVAGQI----DRTLSW---------- 242

Query: 242 LSVGSIMEEASFSIDLQDVKWLQTITKLPILVKGVLTAEDTRIAIQSGAAGIIVSNHGAR 301
                           +DVKWLQTIT LPILVKGV+TAEDTR+A+++GAAGIIVSNHGAR
Sbjct: 243 ----------------KDVKWLQTITTLPILVKGVITAEDTRLAVENGAAGIIVSNHGAR 302

Query: 302 QLDYVPATIVALEEVVKAARGEIPVFLDGGVRRGTDVFKALALGASGIFIGRPVVFSLAA 361
           QLDYVPATI ALEEVVKAARG++PVFLDGGVRRGTDVFKALALGA+G+FIGRPVVFSLAA
Sbjct: 303 QLDYVPATISALEEVVKAARGQLPVFLDGGVRRGTDVFKALALGAAGVFIGRPVVFSLAA 362

Query: 362 EGEAGVRKVLQMLRDEFELTMALSGCRSLQEITRSHIVADWDTPRIVP 410
            GEAGVR VLQMLRDEFELTMALSGC SL +ITR+H++ + D   ++P
Sbjct: 363 AGEAGVRNVLQMLRDEFELTMALSGCTSLADITRNHVITEADKLGVMP 366

BLAST of Cla97C01G024660 vs. ExPASy Swiss-Prot
Match: Q6YT73 (Peroxisomal (S)-2-hydroxy-acid oxidase GLO5 OS=Oryza sativa subsp. japonica OX=39947 GN=GLO5 PE=2 SV=1)

HSP 1 Score: 578.2 bits (1489), Expect = 7.5e-164
Identity = 306/408 (75.00%), Postives = 340/408 (83.33%), Query Frame = 0

Query: 2   EVTNVTEYEAIAKEKLPKMVYDYYASGAEDQWTLKENRNAFSRILFRPRILIDVSKIDMS 61
           E+TNVTEY+AIAK+KLPKM+YDYYASGAED+WTL+ENR AF+RILFRPRILIDVSKIDM+
Sbjct: 3   EITNVTEYQAIAKQKLPKMIYDYYASGAEDEWTLQENREAFARILFRPRILIDVSKIDMA 62

Query: 62  TTVLGFKISMPIMIAPTAMQKMAHPEGEYATARAASAAGTIMTLSSWATSSVEEVASTGP 121
           TTVLGFKISMPIMIAP+AMQKMAHP+GEYATARAASAAGTIMTLSSWATSSVEEVASTGP
Sbjct: 63  TTVLGFKISMPIMIAPSAMQKMAHPDGEYATARAASAAGTIMTLSSWATSSVEEVASTGP 122

Query: 122 GIRFFQLYVYKDRNVVAQLVRRAEKAGFKAIALTVDTPRLGRREADIKNRFTLPPFLTLK 181
           GIRFFQLYVYKDR VV QLVRRAE+AGFKAIALTVDTPRLGRREADIKNRF LPPFLTLK
Sbjct: 123 GIRFFQLYVYKDRRVVEQLVRRAERAGFKAIALTVDTPRLGRREADIKNRFVLPPFLTLK 182

Query: 182 NFEGLDLGKMDKVRLKCHSMLSSVSINNVHDNIFFLCGGVFFYRQTTLDWPHMLRGRSTV 241
           NFEGL+LGKMD+      S L+S           ++ G +      TL W          
Sbjct: 183 NFEGLELGKMDQA---SDSGLAS-----------YVAGQI----DRTLSW---------- 242

Query: 242 LSVGSIMEEASFSIDLQDVKWLQTITKLPILVKGVLTAEDTRIAIQSGAAGIIVSNHGAR 301
                           +DVKWLQTIT LPILVKGV+TAEDTR+A+++GAAGIIVSNHGAR
Sbjct: 243 ----------------KDVKWLQTITTLPILVKGVITAEDTRLAVENGAAGIIVSNHGAR 302

Query: 302 QLDYVPATIVALEEVVKAARGEIPVFLDGGVRRGTDVFKALALGASGIFIGRPVVFSLAA 361
           QLDYVPATI ALEEVVKAARG++PVFLDGGVRRGTDVFKALALGA+G+FIGRPVVFSLAA
Sbjct: 303 QLDYVPATISALEEVVKAARGQLPVFLDGGVRRGTDVFKALALGAAGVFIGRPVVFSLAA 362

Query: 362 EGEAGVRKVLQMLRDEFELTMALSGCRSLQEITRSHIVADWDTPRIVP 410
            GEAGVR VLQMLRDEFELTMALSGC SL +ITR+H++ + D   ++P
Sbjct: 363 AGEAGVRNVLQMLRDEFELTMALSGCTSLADITRNHVITEADKLGVMP 366

BLAST of Cla97C01G024660 vs. ExPASy TrEMBL
Match: A0A1S3AYS3 ((S)-2-hydroxy-acid oxidase OS=Cucumis melo OX=3656 GN=LOC103484358 PE=3 SV=1)

HSP 1 Score: 649.4 bits (1674), Expect = 9.8e-183
Identity = 351/411 (85.40%), Postives = 358/411 (87.10%), Query Frame = 0

Query: 1   MEVTNVTEYEAIAKEKLPKMVYDYYASGAEDQWTLKENRNAFSRILFRPRILIDVSKIDM 60
           ME+TNVTEYEAIAKEKLPKMVYDYYASGAEDQWTLKENRNAFSRILFRPRILIDVSKIDM
Sbjct: 1   MEITNVTEYEAIAKEKLPKMVYDYYASGAEDQWTLKENRNAFSRILFRPRILIDVSKIDM 60

Query: 61  STTVLGFKISMPIMIAPTAMQKMAHPEGEYATARAASAAGTIMTLSSWATSSVEEVASTG 120
           STTVLGFKISMPIMIAPTAMQKMAHPEGEYATARAASAAGTIMTLSSWATSSVEEVASTG
Sbjct: 61  STTVLGFKISMPIMIAPTAMQKMAHPEGEYATARAASAAGTIMTLSSWATSSVEEVASTG 120

Query: 121 PGIRFFQLYVYKDRNVVAQLVRRAEKAGFKAIALTVDTPRLGRREADIKNRFTLPPFLTL 180
           PGIRFFQLYVYKDRNVVAQLVRRAEKAGFKAIALTVDTPRLGRREADIKNRFTLPPFLTL
Sbjct: 121 PGIRFFQLYVYKDRNVVAQLVRRAEKAGFKAIALTVDTPRLGRREADIKNRFTLPPFLTL 180

Query: 181 KNFEGLDLGKMDKVRLKCHSMLSSVSINNVHDNIFFLCGGVFFYRQTTLDWPHMLRGRST 240
           KNFEGLDLGKMDK      S L+S           ++ G +      TL W         
Sbjct: 181 KNFEGLDLGKMDKAD---DSGLAS-----------YVAGQI----DRTLSW--------- 240

Query: 241 VLSVGSIMEEASFSIDLQDVKWLQTITKLPILVKGVLTAEDTRIAIQSGAAGIIVSNHGA 300
                            QDVKWLQTITKLPILVKGVLTAEDTRIAIQSGAAGIIVSNHGA
Sbjct: 241 -----------------QDVKWLQTITKLPILVKGVLTAEDTRIAIQSGAAGIIVSNHGA 300

Query: 301 RQLDYVPATIVALEEVVKAARGEIPVFLDGGVRRGTDVFKALALGASGIFIGRPVVFSLA 360
           RQLDYVPATIVALEEVVKAARGE+PVFLDGGVRRGTDVFKALALGASGIFIGRPVVFSLA
Sbjct: 301 RQLDYVPATIVALEEVVKAARGEVPVFLDGGVRRGTDVFKALALGASGIFIGRPVVFSLA 360

Query: 361 AEGEAGVRKVLQMLRDEFELTMALSGCRSLQEITRSHIVADWDTPRIVPRL 412
           AEGEAGVRKVLQMLRDEFELTMALSGCRSLQEITRSHI+ADWDTPRIVPRL
Sbjct: 361 AEGEAGVRKVLQMLRDEFELTMALSGCRSLQEITRSHILADWDTPRIVPRL 367

BLAST of Cla97C01G024660 vs. ExPASy TrEMBL
Match: A0A5D3CN84 ((S)-2-hydroxy-acid oxidase OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold255G009120 PE=3 SV=1)

HSP 1 Score: 649.4 bits (1674), Expect = 9.8e-183
Identity = 351/411 (85.40%), Postives = 358/411 (87.10%), Query Frame = 0

Query: 1   MEVTNVTEYEAIAKEKLPKMVYDYYASGAEDQWTLKENRNAFSRILFRPRILIDVSKIDM 60
           ME+TNVTEYEAIAKEKLPKMVYDYYASGAEDQWTLKENRNAFSRILFRPRILIDVSKIDM
Sbjct: 1   MEITNVTEYEAIAKEKLPKMVYDYYASGAEDQWTLKENRNAFSRILFRPRILIDVSKIDM 60

Query: 61  STTVLGFKISMPIMIAPTAMQKMAHPEGEYATARAASAAGTIMTLSSWATSSVEEVASTG 120
           STTVLGFKISMPIMIAPTAMQKMAHPEGEYATARAASAAGTIMTLSSWATSSVEEVASTG
Sbjct: 61  STTVLGFKISMPIMIAPTAMQKMAHPEGEYATARAASAAGTIMTLSSWATSSVEEVASTG 120

Query: 121 PGIRFFQLYVYKDRNVVAQLVRRAEKAGFKAIALTVDTPRLGRREADIKNRFTLPPFLTL 180
           PGIRFFQLYVYKDRNVVAQLVRRAEKAGFKAIALTVDTPRLGRREADIKNRFTLPPFLTL
Sbjct: 121 PGIRFFQLYVYKDRNVVAQLVRRAEKAGFKAIALTVDTPRLGRREADIKNRFTLPPFLTL 180

Query: 181 KNFEGLDLGKMDKVRLKCHSMLSSVSINNVHDNIFFLCGGVFFYRQTTLDWPHMLRGRST 240
           KNFEGLDLGKMDK      S L+S           ++ G +      TL W         
Sbjct: 181 KNFEGLDLGKMDKAD---DSGLAS-----------YVAGQI----DRTLSW--------- 240

Query: 241 VLSVGSIMEEASFSIDLQDVKWLQTITKLPILVKGVLTAEDTRIAIQSGAAGIIVSNHGA 300
                            QDVKWLQTITKLPILVKGVLTAEDTRIAIQSGAAGIIVSNHGA
Sbjct: 241 -----------------QDVKWLQTITKLPILVKGVLTAEDTRIAIQSGAAGIIVSNHGA 300

Query: 301 RQLDYVPATIVALEEVVKAARGEIPVFLDGGVRRGTDVFKALALGASGIFIGRPVVFSLA 360
           RQLDYVPATIVALEEVVKAARGE+PVFLDGGVRRGTDVFKALALGASGIFIGRPVVFSLA
Sbjct: 301 RQLDYVPATIVALEEVVKAARGEVPVFLDGGVRRGTDVFKALALGASGIFIGRPVVFSLA 360

Query: 361 AEGEAGVRKVLQMLRDEFELTMALSGCRSLQEITRSHIVADWDTPRIVPRL 412
           AEGEAGVRKVLQMLRDEFELTMALSGCRSLQEITRSHI+ADWDTPRIVPRL
Sbjct: 361 AEGEAGVRKVLQMLRDEFELTMALSGCRSLQEITRSHILADWDTPRIVPRL 367

BLAST of Cla97C01G024660 vs. ExPASy TrEMBL
Match: A0A5A7UFQ2 ((S)-2-hydroxy-acid oxidase OS=Cucumis melo var. makuwa OX=1194695 GN=E6C27_scaffold120G001360 PE=3 SV=1)

HSP 1 Score: 646.7 bits (1667), Expect = 6.4e-182
Identity = 349/411 (84.91%), Postives = 358/411 (87.10%), Query Frame = 0

Query: 1   MEVTNVTEYEAIAKEKLPKMVYDYYASGAEDQWTLKENRNAFSRILFRPRILIDVSKIDM 60
           ME+TNVTEYEAIAKEKLPKMVYDYYASGAEDQWTLKENRNAFSRILFRPRILIDVSKIDM
Sbjct: 1   MEITNVTEYEAIAKEKLPKMVYDYYASGAEDQWTLKENRNAFSRILFRPRILIDVSKIDM 60

Query: 61  STTVLGFKISMPIMIAPTAMQKMAHPEGEYATARAASAAGTIMTLSSWATSSVEEVASTG 120
           STTVLGFKISMPIMIAPTAMQKMAHPEGEYATARAASAAGTIMTLSSWATSSVEEVASTG
Sbjct: 61  STTVLGFKISMPIMIAPTAMQKMAHPEGEYATARAASAAGTIMTLSSWATSSVEEVASTG 120

Query: 121 PGIRFFQLYVYKDRNVVAQLVRRAEKAGFKAIALTVDTPRLGRREADIKNRFTLPPFLTL 180
           PGIRFFQLYVYKDRNVVAQLVRRAEKAGFKAIALTVDTPRLGRREADIKNRFTLPPFLTL
Sbjct: 121 PGIRFFQLYVYKDRNVVAQLVRRAEKAGFKAIALTVDTPRLGRREADIKNRFTLPPFLTL 180

Query: 181 KNFEGLDLGKMDKVRLKCHSMLSSVSINNVHDNIFFLCGGVFFYRQTTLDWPHMLRGRST 240
           KNFEGLDLGKMDK      S L+S           ++ G +      TL W         
Sbjct: 181 KNFEGLDLGKMDKAD---DSGLAS-----------YVAGQI----DRTLSW--------- 240

Query: 241 VLSVGSIMEEASFSIDLQDVKWLQTITKLPILVKGVLTAEDTRIAIQSGAAGIIVSNHGA 300
                            QDVKWLQTITKLPILVKGVLTAED++IAIQSGAAGIIVSNHGA
Sbjct: 241 -----------------QDVKWLQTITKLPILVKGVLTAEDSKIAIQSGAAGIIVSNHGA 300

Query: 301 RQLDYVPATIVALEEVVKAARGEIPVFLDGGVRRGTDVFKALALGASGIFIGRPVVFSLA 360
           RQLDYVPATIVALEEVVKAARGE+PVFLDGGVRRGTDVFKALALGASGIFIGRPVVFSLA
Sbjct: 301 RQLDYVPATIVALEEVVKAARGEVPVFLDGGVRRGTDVFKALALGASGIFIGRPVVFSLA 360

Query: 361 AEGEAGVRKVLQMLRDEFELTMALSGCRSLQEITRSHIVADWDTPRIVPRL 412
           AEGEAGVRKVLQMLRDEFELTMALSGCRSLQEITRSHI+ADWDTPRIVPRL
Sbjct: 361 AEGEAGVRKVLQMLRDEFELTMALSGCRSLQEITRSHILADWDTPRIVPRL 367

BLAST of Cla97C01G024660 vs. ExPASy TrEMBL
Match: A0A6J1ISV7 ((S)-2-hydroxy-acid oxidase OS=Cucurbita maxima OX=3661 GN=LOC111478373 PE=3 SV=1)

HSP 1 Score: 646.0 bits (1665), Expect = 1.1e-181
Identity = 347/411 (84.43%), Postives = 358/411 (87.10%), Query Frame = 0

Query: 1   MEVTNVTEYEAIAKEKLPKMVYDYYASGAEDQWTLKENRNAFSRILFRPRILIDVSKIDM 60
           ME+TNVTEYEAIAKEKLPKMVYDYYASGAEDQWTLKENRNAFSRILFRPRILIDVSKIDM
Sbjct: 1   MEITNVTEYEAIAKEKLPKMVYDYYASGAEDQWTLKENRNAFSRILFRPRILIDVSKIDM 60

Query: 61  STTVLGFKISMPIMIAPTAMQKMAHPEGEYATARAASAAGTIMTLSSWATSSVEEVASTG 120
           STTVLGFKISMPIMIAPTAMQKMAHPEGEYATARAASAAGTIMTLSSWATSSVEEVASTG
Sbjct: 61  STTVLGFKISMPIMIAPTAMQKMAHPEGEYATARAASAAGTIMTLSSWATSSVEEVASTG 120

Query: 121 PGIRFFQLYVYKDRNVVAQLVRRAEKAGFKAIALTVDTPRLGRREADIKNRFTLPPFLTL 180
           PGIRFFQLYVYKDRNVVAQLVRRAEKAGFKAIALTVDTPRLGRREADIKNRFTLPPFLTL
Sbjct: 121 PGIRFFQLYVYKDRNVVAQLVRRAEKAGFKAIALTVDTPRLGRREADIKNRFTLPPFLTL 180

Query: 181 KNFEGLDLGKMDKVRLKCHSMLSSVSINNVHDNIFFLCGGVFFYRQTTLDWPHMLRGRST 240
           KNFEGLDLGKMD+      S L+S           ++ G +      TL W         
Sbjct: 181 KNFEGLDLGKMDQAD---DSGLAS-----------YVAGQI----DRTLSW--------- 240

Query: 241 VLSVGSIMEEASFSIDLQDVKWLQTITKLPILVKGVLTAEDTRIAIQSGAAGIIVSNHGA 300
                            QDVKWLQTITKLPILVKGVLTAEDTRIA+QSGAAGIIVSNHGA
Sbjct: 241 -----------------QDVKWLQTITKLPILVKGVLTAEDTRIAVQSGAAGIIVSNHGA 300

Query: 301 RQLDYVPATIVALEEVVKAARGEIPVFLDGGVRRGTDVFKALALGASGIFIGRPVVFSLA 360
           RQLDYVPATI+ALEEVVKAARGE+PVFLDGGVRRGTDVFKALALGASGIFIGRPVVFSLA
Sbjct: 301 RQLDYVPATIMALEEVVKAARGEVPVFLDGGVRRGTDVFKALALGASGIFIGRPVVFSLA 360

Query: 361 AEGEAGVRKVLQMLRDEFELTMALSGCRSLQEITRSHIVADWDTPRIVPRL 412
           AEGEAGVRKVLQMLRDEFELTMALSGCRSLQEITR+HIVADWDTPR+VPRL
Sbjct: 361 AEGEAGVRKVLQMLRDEFELTMALSGCRSLQEITRNHIVADWDTPRVVPRL 367

BLAST of Cla97C01G024660 vs. ExPASy TrEMBL
Match: A0A6J1EGX3 ((S)-2-hydroxy-acid oxidase OS=Cucurbita moschata OX=3662 GN=LOC111433275 PE=3 SV=1)

HSP 1 Score: 646.0 bits (1665), Expect = 1.1e-181
Identity = 347/411 (84.43%), Postives = 358/411 (87.10%), Query Frame = 0

Query: 1   MEVTNVTEYEAIAKEKLPKMVYDYYASGAEDQWTLKENRNAFSRILFRPRILIDVSKIDM 60
           ME+TNVTEYEAIAKEKLPKMVYDYYASGAEDQWTLKENRNAFSRILFRPRILIDVSKIDM
Sbjct: 1   MEITNVTEYEAIAKEKLPKMVYDYYASGAEDQWTLKENRNAFSRILFRPRILIDVSKIDM 60

Query: 61  STTVLGFKISMPIMIAPTAMQKMAHPEGEYATARAASAAGTIMTLSSWATSSVEEVASTG 120
           STTVLGFKISMPIMIAPTAMQKMAHPEGEYATARAASAAGTIMTLSSWATSSVEEVASTG
Sbjct: 61  STTVLGFKISMPIMIAPTAMQKMAHPEGEYATARAASAAGTIMTLSSWATSSVEEVASTG 120

Query: 121 PGIRFFQLYVYKDRNVVAQLVRRAEKAGFKAIALTVDTPRLGRREADIKNRFTLPPFLTL 180
           PGIRFFQLYVYKDRNVVAQLVRRAEKAGFKAIALTVDTPRLGRREADIKNRFTLPPFLTL
Sbjct: 121 PGIRFFQLYVYKDRNVVAQLVRRAEKAGFKAIALTVDTPRLGRREADIKNRFTLPPFLTL 180

Query: 181 KNFEGLDLGKMDKVRLKCHSMLSSVSINNVHDNIFFLCGGVFFYRQTTLDWPHMLRGRST 240
           KNFEGLDLGKMD+      S L+S           ++ G +      TL W         
Sbjct: 181 KNFEGLDLGKMDQAD---DSGLAS-----------YVAGQI----DRTLSW--------- 240

Query: 241 VLSVGSIMEEASFSIDLQDVKWLQTITKLPILVKGVLTAEDTRIAIQSGAAGIIVSNHGA 300
                            QDVKWLQTITKLPILVKGVLTAEDTRIA+QSGAAGIIVSNHGA
Sbjct: 241 -----------------QDVKWLQTITKLPILVKGVLTAEDTRIAVQSGAAGIIVSNHGA 300

Query: 301 RQLDYVPATIVALEEVVKAARGEIPVFLDGGVRRGTDVFKALALGASGIFIGRPVVFSLA 360
           RQLDYVPATI+ALEEVVKAARGE+PVFLDGGVRRGTDVFKALALGASGIFIGRPVVFSLA
Sbjct: 301 RQLDYVPATIMALEEVVKAARGEVPVFLDGGVRRGTDVFKALALGASGIFIGRPVVFSLA 360

Query: 361 AEGEAGVRKVLQMLRDEFELTMALSGCRSLQEITRSHIVADWDTPRIVPRL 412
           AEGEAGVRKVLQMLRDEFELTMALSGCRSLQEITR+HIVADWDTPR+VPRL
Sbjct: 361 AEGEAGVRKVLQMLRDEFELTMALSGCRSLQEITRNHIVADWDTPRVVPRL 367

BLAST of Cla97C01G024660 vs. TAIR 10
Match: AT3G14420.1 (Aldolase-type TIM barrel family protein )

HSP 1 Score: 600.9 bits (1548), Expect = 7.7e-172
Identity = 322/411 (78.35%), Postives = 346/411 (84.18%), Query Frame = 0

Query: 1   MEVTNVTEYEAIAKEKLPKMVYDYYASGAEDQWTLKENRNAFSRILFRPRILIDVSKIDM 60
           ME+TNVTEY+AIAK+KLPKMVYDYYASGAEDQWTL+ENRNAF+RILFRPRILIDVSKIDM
Sbjct: 1   MEITNVTEYDAIAKQKLPKMVYDYYASGAEDQWTLQENRNAFARILFRPRILIDVSKIDM 60

Query: 61  STTVLGFKISMPIMIAPTAMQKMAHPEGEYATARAASAAGTIMTLSSWATSSVEEVASTG 120
           +TTVLGFKISMPIM+APTAMQKMAHP+GEYATARAASAAGTIMTLSSWATSSVEEVASTG
Sbjct: 61  TTTVLGFKISMPIMVAPTAMQKMAHPDGEYATARAASAAGTIMTLSSWATSSVEEVASTG 120

Query: 121 PGIRFFQLYVYKDRNVVAQLVRRAEKAGFKAIALTVDTPRLGRREADIKNRFTLPPFLTL 180
           PGIRFFQLYVYK+RNVV QLVRRAE+AGFKAIALTVDTPRLGRRE+DIKNRFTLPP LTL
Sbjct: 121 PGIRFFQLYVYKNRNVVEQLVRRAERAGFKAIALTVDTPRLGRRESDIKNRFTLPPNLTL 180

Query: 181 KNFEGLDLGKMDKVRLKCHSMLSSVSINNVHDNIFFLCGGVFFYRQTTLDWPHMLRGRST 240
           KNFEGLDLGKMD+      S L+S           ++ G +      TL W         
Sbjct: 181 KNFEGLDLGKMDEAN---DSGLAS-----------YVAGQI----DRTLSW--------- 240

Query: 241 VLSVGSIMEEASFSIDLQDVKWLQTITKLPILVKGVLTAEDTRIAIQSGAAGIIVSNHGA 300
                            +DV+WLQTITKLPILVKGVLT ED RIAIQ+GAAGIIVSNHGA
Sbjct: 241 -----------------KDVQWLQTITKLPILVKGVLTGEDARIAIQAGAAGIIVSNHGA 300

Query: 301 RQLDYVPATIVALEEVVKAARGEIPVFLDGGVRRGTDVFKALALGASGIFIGRPVVFSLA 360
           RQLDYVPATI ALEEVVKA +G IPVFLDGGVRRGTDVFKALALGASGIFIGRPVVFSLA
Sbjct: 301 RQLDYVPATISALEEVVKATQGRIPVFLDGGVRRGTDVFKALALGASGIFIGRPVVFSLA 360

Query: 361 AEGEAGVRKVLQMLRDEFELTMALSGCRSLQEITRSHIVADWDTPRIVPRL 412
           AEGEAGVRKVLQMLRDEFELTMALSGCRSL+EI+R+HI  +WDTPR   RL
Sbjct: 361 AEGEAGVRKVLQMLRDEFELTMALSGCRSLKEISRNHITTEWDTPRPSARL 367

BLAST of Cla97C01G024660 vs. TAIR 10
Match: AT3G14420.2 (Aldolase-type TIM barrel family protein )

HSP 1 Score: 600.9 bits (1548), Expect = 7.7e-172
Identity = 322/411 (78.35%), Postives = 346/411 (84.18%), Query Frame = 0

Query: 1   MEVTNVTEYEAIAKEKLPKMVYDYYASGAEDQWTLKENRNAFSRILFRPRILIDVSKIDM 60
           ME+TNVTEY+AIAK+KLPKMVYDYYASGAEDQWTL+ENRNAF+RILFRPRILIDVSKIDM
Sbjct: 1   MEITNVTEYDAIAKQKLPKMVYDYYASGAEDQWTLQENRNAFARILFRPRILIDVSKIDM 60

Query: 61  STTVLGFKISMPIMIAPTAMQKMAHPEGEYATARAASAAGTIMTLSSWATSSVEEVASTG 120
           +TTVLGFKISMPIM+APTAMQKMAHP+GEYATARAASAAGTIMTLSSWATSSVEEVASTG
Sbjct: 61  TTTVLGFKISMPIMVAPTAMQKMAHPDGEYATARAASAAGTIMTLSSWATSSVEEVASTG 120

Query: 121 PGIRFFQLYVYKDRNVVAQLVRRAEKAGFKAIALTVDTPRLGRREADIKNRFTLPPFLTL 180
           PGIRFFQLYVYK+RNVV QLVRRAE+AGFKAIALTVDTPRLGRRE+DIKNRFTLPP LTL
Sbjct: 121 PGIRFFQLYVYKNRNVVEQLVRRAERAGFKAIALTVDTPRLGRRESDIKNRFTLPPNLTL 180

Query: 181 KNFEGLDLGKMDKVRLKCHSMLSSVSINNVHDNIFFLCGGVFFYRQTTLDWPHMLRGRST 240
           KNFEGLDLGKMD+      S L+S           ++ G +      TL W         
Sbjct: 181 KNFEGLDLGKMDEAN---DSGLAS-----------YVAGQI----DRTLSW--------- 240

Query: 241 VLSVGSIMEEASFSIDLQDVKWLQTITKLPILVKGVLTAEDTRIAIQSGAAGIIVSNHGA 300
                            +DV+WLQTITKLPILVKGVLT ED RIAIQ+GAAGIIVSNHGA
Sbjct: 241 -----------------KDVQWLQTITKLPILVKGVLTGEDARIAIQAGAAGIIVSNHGA 300

Query: 301 RQLDYVPATIVALEEVVKAARGEIPVFLDGGVRRGTDVFKALALGASGIFIGRPVVFSLA 360
           RQLDYVPATI ALEEVVKA +G IPVFLDGGVRRGTDVFKALALGASGIFIGRPVVFSLA
Sbjct: 301 RQLDYVPATISALEEVVKATQGRIPVFLDGGVRRGTDVFKALALGASGIFIGRPVVFSLA 360

Query: 361 AEGEAGVRKVLQMLRDEFELTMALSGCRSLQEITRSHIVADWDTPRIVPRL 412
           AEGEAGVRKVLQMLRDEFELTMALSGCRSL+EI+R+HI  +WDTPR   RL
Sbjct: 361 AEGEAGVRKVLQMLRDEFELTMALSGCRSLKEISRNHITTEWDTPRPSARL 367

BLAST of Cla97C01G024660 vs. TAIR 10
Match: AT3G14420.3 (Aldolase-type TIM barrel family protein )

HSP 1 Score: 598.2 bits (1541), Expect = 5.0e-171
Identity = 321/411 (78.10%), Postives = 345/411 (83.94%), Query Frame = 0

Query: 1   MEVTNVTEYEAIAKEKLPKMVYDYYASGAEDQWTLKENRNAFSRILFRPRILIDVSKIDM 60
           ME+TNVTEY+AIAK+KLPKMVYDYYASGAEDQWTL+ENRNAF+RILFRPRILIDVSKIDM
Sbjct: 1   MEITNVTEYDAIAKQKLPKMVYDYYASGAEDQWTLQENRNAFARILFRPRILIDVSKIDM 60

Query: 61  STTVLGFKISMPIMIAPTAMQKMAHPEGEYATARAASAAGTIMTLSSWATSSVEEVASTG 120
           +TTVLGFKISMPIM+APTAMQKMAHP+GEYATARAASAAGTIMTLSSWATSSVEEVASTG
Sbjct: 61  TTTVLGFKISMPIMVAPTAMQKMAHPDGEYATARAASAAGTIMTLSSWATSSVEEVASTG 120

Query: 121 PGIRFFQLYVYKDRNVVAQLVRRAEKAGFKAIALTVDTPRLGRREADIKNRFTLPPFLTL 180
           PGIRFFQLYVYK+RNVV QLVRRAE+AGFKAIALTVDTPRLGRRE+DIKNRFTLPP LTL
Sbjct: 121 PGIRFFQLYVYKNRNVVEQLVRRAERAGFKAIALTVDTPRLGRRESDIKNRFTLPPNLTL 180

Query: 181 KNFEGLDLGKMDKVRLKCHSMLSSVSINNVHDNIFFLCGGVFFYRQTTLDWPHMLRGRST 240
           KNFEGLDLGKMD+      S L+S           ++ G +      TL W         
Sbjct: 181 KNFEGLDLGKMDEAN---DSGLAS-----------YVAGQI----DRTLSW--------- 240

Query: 241 VLSVGSIMEEASFSIDLQDVKWLQTITKLPILVKGVLTAEDTRIAIQSGAAGIIVSNHGA 300
                            +DV+WLQTITKLPILVKGVLT ED  IAIQ+GAAGIIVSNHGA
Sbjct: 241 -----------------KDVQWLQTITKLPILVKGVLTGEDGEIAIQAGAAGIIVSNHGA 300

Query: 301 RQLDYVPATIVALEEVVKAARGEIPVFLDGGVRRGTDVFKALALGASGIFIGRPVVFSLA 360
           RQLDYVPATI ALEEVVKA +G IPVFLDGGVRRGTDVFKALALGASGIFIGRPVVFSLA
Sbjct: 301 RQLDYVPATISALEEVVKATQGRIPVFLDGGVRRGTDVFKALALGASGIFIGRPVVFSLA 360

Query: 361 AEGEAGVRKVLQMLRDEFELTMALSGCRSLQEITRSHIVADWDTPRIVPRL 412
           AEGEAGVRKVLQMLRDEFELTMALSGCRSL+EI+R+HI  +WDTPR   RL
Sbjct: 361 AEGEAGVRKVLQMLRDEFELTMALSGCRSLKEISRNHITTEWDTPRPSARL 367

BLAST of Cla97C01G024660 vs. TAIR 10
Match: AT3G14415.2 (Aldolase-type TIM barrel family protein )

HSP 1 Score: 598.2 bits (1541), Expect = 5.0e-171
Identity = 316/411 (76.89%), Postives = 343/411 (83.45%), Query Frame = 0

Query: 1   MEVTNVTEYEAIAKEKLPKMVYDYYASGAEDQWTLKENRNAFSRILFRPRILIDVSKIDM 60
           ME+TNVTEY+AIAK KLPKMVYDYYASGAEDQWTL+ENRNAF+RILFRPRILIDV+KIDM
Sbjct: 1   MEITNVTEYDAIAKAKLPKMVYDYYASGAEDQWTLQENRNAFARILFRPRILIDVNKIDM 60

Query: 61  STTVLGFKISMPIMIAPTAMQKMAHPEGEYATARAASAAGTIMTLSSWATSSVEEVASTG 120
           +TTVLGFKISMPIM+APTA QKMAHP+GEYATARAASAAGTIMTLSSWATSSVEEVASTG
Sbjct: 61  ATTVLGFKISMPIMVAPTAFQKMAHPDGEYATARAASAAGTIMTLSSWATSSVEEVASTG 120

Query: 121 PGIRFFQLYVYKDRNVVAQLVRRAEKAGFKAIALTVDTPRLGRREADIKNRFTLPPFLTL 180
           PGIRFFQLYVYK+R VV QLVRRAEKAGFKAIALTVDTPRLGRRE+DIKNRFTLPP LTL
Sbjct: 121 PGIRFFQLYVYKNRKVVEQLVRRAEKAGFKAIALTVDTPRLGRRESDIKNRFTLPPNLTL 180

Query: 181 KNFEGLDLGKMDKVRLKCHSMLSSVSINNVHDNIFFLCGGVFFYRQTTLDWPHMLRGRST 240
           KNFEGLDLGKMD+  +          I N      ++ G +      TL W         
Sbjct: 181 KNFEGLDLGKMDEASID--------QIANDSGLASYVAGQI----DRTLSW--------- 240

Query: 241 VLSVGSIMEEASFSIDLQDVKWLQTITKLPILVKGVLTAEDTRIAIQSGAAGIIVSNHGA 300
                            +D++WLQTIT +PILVKGVLT ED RIAIQ+GAAGIIVSNHGA
Sbjct: 241 -----------------KDIQWLQTITNMPILVKGVLTGEDARIAIQAGAAGIIVSNHGA 300

Query: 301 RQLDYVPATIVALEEVVKAARGEIPVFLDGGVRRGTDVFKALALGASGIFIGRPVVFSLA 360
           RQLDYVPATI ALEEVVKA +G +PVFLDGGVRRGTDVFKALALGASGIFIGRPVVF+LA
Sbjct: 301 RQLDYVPATISALEEVVKATQGRVPVFLDGGVRRGTDVFKALALGASGIFIGRPVVFALA 360

Query: 361 AEGEAGVRKVLQMLRDEFELTMALSGCRSLQEITRSHIVADWDTPRIVPRL 412
           AEGEAGV+KVLQMLRDEFELTMALSGCRSL EITR+HIV +WDTPR +PRL
Sbjct: 361 AEGEAGVKKVLQMLRDEFELTMALSGCRSLSEITRNHIVTEWDTPRHLPRL 373

BLAST of Cla97C01G024660 vs. TAIR 10
Match: AT3G14415.1 (Aldolase-type TIM barrel family protein )

HSP 1 Score: 596.7 bits (1537), Expect = 1.5e-170
Identity = 317/411 (77.13%), Postives = 344/411 (83.70%), Query Frame = 0

Query: 1   MEVTNVTEYEAIAKEKLPKMVYDYYASGAEDQWTLKENRNAFSRILFRPRILIDVSKIDM 60
           ME+TNVTEY+AIAK KLPKMVYDYYASGAEDQWTL+ENRNAF+RILFRPRILIDV+KIDM
Sbjct: 1   MEITNVTEYDAIAKAKLPKMVYDYYASGAEDQWTLQENRNAFARILFRPRILIDVNKIDM 60

Query: 61  STTVLGFKISMPIMIAPTAMQKMAHPEGEYATARAASAAGTIMTLSSWATSSVEEVASTG 120
           +TTVLGFKISMPIM+APTA QKMAHP+GEYATARAASAAGTIMTLSSWATSSVEEVASTG
Sbjct: 61  ATTVLGFKISMPIMVAPTAFQKMAHPDGEYATARAASAAGTIMTLSSWATSSVEEVASTG 120

Query: 121 PGIRFFQLYVYKDRNVVAQLVRRAEKAGFKAIALTVDTPRLGRREADIKNRFTLPPFLTL 180
           PGIRFFQLYVYK+R VV QLVRRAEKAGFKAIALTVDTPRLGRRE+DIKNRFTLPP LTL
Sbjct: 121 PGIRFFQLYVYKNRKVVEQLVRRAEKAGFKAIALTVDTPRLGRRESDIKNRFTLPPNLTL 180

Query: 181 KNFEGLDLGKMDKVRLKCHSMLSSVSINNVHDNIFFLCGGVFFYRQTTLDWPHMLRGRST 240
           KNFEGLDLGKMD+      S L+S           ++ G +      TL W         
Sbjct: 181 KNFEGLDLGKMDEAN---DSGLAS-----------YVAGQI----DRTLSW--------- 240

Query: 241 VLSVGSIMEEASFSIDLQDVKWLQTITKLPILVKGVLTAEDTRIAIQSGAAGIIVSNHGA 300
                            +D++WLQTIT +PILVKGVLT ED RIAIQ+GAAGIIVSNHGA
Sbjct: 241 -----------------KDIQWLQTITNMPILVKGVLTGEDARIAIQAGAAGIIVSNHGA 300

Query: 301 RQLDYVPATIVALEEVVKAARGEIPVFLDGGVRRGTDVFKALALGASGIFIGRPVVFSLA 360
           RQLDYVPATI ALEEVVKA +G +PVFLDGGVRRGTDVFKALALGASGIFIGRPVVF+LA
Sbjct: 301 RQLDYVPATISALEEVVKATQGRVPVFLDGGVRRGTDVFKALALGASGIFIGRPVVFALA 360

Query: 361 AEGEAGVRKVLQMLRDEFELTMALSGCRSLQEITRSHIVADWDTPRIVPRL 412
           AEGEAGV+KVLQMLRDEFELTMALSGCRSL EITR+HIV +WDTPR +PRL
Sbjct: 361 AEGEAGVKKVLQMLRDEFELTMALSGCRSLSEITRNHIVTEWDTPRHLPRL 367

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
XP_008439620.12.0e-18285.40PREDICTED: peroxisomal (S)-2-hydroxy-acid oxidase GLO1 [Cucumis melo] >XP_008439... [more]
XP_038883246.13.5e-18284.91(S)-2-hydroxy-acid oxidase GLO1 [Benincasa hispida][more]
KAA0052535.11.3e-18184.91peroxisomal (S)-2-hydroxy-acid oxidase GLO1 [Cucumis melo var. makuwa][more]
XP_022926037.12.2e-18184.43(S)-2-hydroxy-acid oxidase GLO1 [Cucurbita moschata] >XP_022926038.1 (S)-2-hydro... [more]
KAG7034728.12.2e-18184.43Peroxisomal (S)-2-hydroxy-acid oxidase GLO1 [Cucurbita argyrosperma subsp. argyr... [more]
Match NameE-valueIdentityDescription
Q9LRR91.1e-17078.35(S)-2-hydroxy-acid oxidase GLO1 OS=Arabidopsis thaliana OX=3702 GN=GLO1 PE=1 SV=... [more]
P054141.4e-17077.97Peroxisomal (S)-2-hydroxy-acid oxidase OS=Spinacia oleracea OX=3562 PE=1 SV=1[more]
Q9LRS02.0e-16977.13(S)-2-hydroxy-acid oxidase GLO2 OS=Arabidopsis thaliana OX=3702 GN=GLO2 PE=1 SV=... [more]
B8B7C57.5e-16475.00Peroxisomal (S)-2-hydroxy-acid oxidase GLO5 OS=Oryza sativa subsp. indica OX=399... [more]
Q6YT737.5e-16475.00Peroxisomal (S)-2-hydroxy-acid oxidase GLO5 OS=Oryza sativa subsp. japonica OX=3... [more]
Match NameE-valueIdentityDescription
A0A1S3AYS39.8e-18385.40(S)-2-hydroxy-acid oxidase OS=Cucumis melo OX=3656 GN=LOC103484358 PE=3 SV=1[more]
A0A5D3CN849.8e-18385.40(S)-2-hydroxy-acid oxidase OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaff... [more]
A0A5A7UFQ26.4e-18284.91(S)-2-hydroxy-acid oxidase OS=Cucumis melo var. makuwa OX=1194695 GN=E6C27_scaff... [more]
A0A6J1ISV71.1e-18184.43(S)-2-hydroxy-acid oxidase OS=Cucurbita maxima OX=3661 GN=LOC111478373 PE=3 SV=1[more]
A0A6J1EGX31.1e-18184.43(S)-2-hydroxy-acid oxidase OS=Cucurbita moschata OX=3662 GN=LOC111433275 PE=3 SV... [more]
Match NameE-valueIdentityDescription
AT3G14420.17.7e-17278.35Aldolase-type TIM barrel family protein [more]
AT3G14420.27.7e-17278.35Aldolase-type TIM barrel family protein [more]
AT3G14420.35.0e-17178.10Aldolase-type TIM barrel family protein [more]
AT3G14415.25.0e-17176.89Aldolase-type TIM barrel family protein [more]
AT3G14415.11.5e-17077.13Aldolase-type TIM barrel family protein [more]
InterPro
Analysis Name: InterPro Annotations of Watermelon (97103) v2.5
Date Performed: 2022-01-31
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR012133Alpha-hydroxy acid dehydrogenase, FMN-dependentPIRSFPIRSF000138Al-hdrx_acd_dhcoord: 1..403
e-value: 1.3E-132
score: 440.3
IPR012133Alpha-hydroxy acid dehydrogenase, FMN-dependentCDDcd02809alpha_hydroxyacid_oxid_FMNcoord: 8..395
e-value: 9.64294E-157
score: 442.272
IPR000262FMN-dependent dehydrogenasePFAMPF01070FMN_dhcoord: 13..398
e-value: 9.5E-132
score: 439.2
IPR013785Aldolase-type TIM barrelGENE3D3.20.20.70Aldolase class Icoord: 1..410
e-value: 1.2E-168
score: 562.5
NoneNo IPR availablePIRSRPIRSR000138-2PIRSR000138-2coord: 2..400
e-value: 5.3E-143
score: 474.2
NoneNo IPR availablePIRSRPIRSR000138-1PIRSR000138-1coord: 2..400
e-value: 5.3E-143
score: 474.2
NoneNo IPR availablePANTHERPTHR10578:SF129ALPHA-HYDROXY ACID DEHYDROGENASE, FMN-DEPENDENT, ALDOLASE-TYPE TIM BARREL-RELATEDcoord: 1..194
coord: 255..408
NoneNo IPR availablePANTHERPTHR10578S -2-HYDROXY-ACID OXIDASE-RELATEDcoord: 1..194
coord: 255..408
NoneNo IPR availableSUPERFAMILY51395FMN-linked oxidoreductasescoord: 3..399
IPR008259FMN-dependent alpha-hydroxy acid dehydrogenase, active sitePROSITEPS00557FMN_HYDROXY_ACID_DH_1coord: 296..302
IPR037396FMN hydroxy acid dehydrogenase domainPROSITEPS51349FMN_HYDROXY_ACID_DH_2coord: 1..403
score: 116.525375

Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
Cla97C01G024660.2Cla97C01G024660.2mRNA


GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
biological_process GO:0009854 oxidative photosynthetic carbon pathway
cellular_component GO:0005777 peroxisome
molecular_function GO:0010181 FMN binding
molecular_function GO:0003973 (S)-2-hydroxy-acid oxidase activity
molecular_function GO:0003824 catalytic activity
molecular_function GO:0016491 oxidoreductase activity