Cla97C01G024530 (gene) Watermelon (97103) v2.5

Overview
NameCla97C01G024530
Typegene
OrganismCitrullus lanatus subsp. vulgaris cv. 97103 (Watermelon (97103) v2.5)
Descriptionalpha-1,2-Mannosidase
LocationCla97Chr01: 35571074 .. 35576722 (-)
RNA-Seq ExpressionCla97C01G024530
SyntenyCla97C01G024530
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: polypeptideexonCDS
Hold the cursor over a type above to highlight its positions in the sequence below.
ATGTCGAGTTCTCTACCCTACTCTGTCAAGGATGTTCATTACGACAATGCTAAGTTTCGACAGAGATCCTACTTCAAGGTCTTTCTATGCTAGATCTTGACTTATTGCTCCATTTCGATTTCATGAAATCTGATTGCTTATAAGCCACTCCGTTATGCGATTTTTTTATTCGGTTAATGTTTGGATGAATTGGTAGCTTTCTAGTTACATTCTAGCGACGAGTATTTTTTAATTCTTATTCGTTCCTTGAACTCTGATTGTGCCTGAAACAACTTTAGATTCTGGAATGGAGCAAAACTCTACGGAATTGAACATCTGGATTTGTTTGAATAGTCATTTTCGTCGGCAATGCGTGCATAATCAGTGGAAATTATCATAGAAACGCCAGATTGAGGATATTTGTGTTTTGCGAAATAATTAGGAGCTATGTTGTTAGGTCCTTTGTGATTGATGTGAAATTATACAACGTTGTTCATAAACGTATAGTCTCTATTGGTGGTTATACACGCTTCTTCTACTGTAAATTTCCGACTTGCCAATACTTTTGGATGACTGCCCGAACAATATGGAAAAAACAGAGAAGAGCATTTTTCAAGGAAGAATAAGTTTACATCTCTTTTTCACATGCCTCAAACTACAGAAGATTTTTAATGACTAGTAATTTCTCCTTCCAAAATTCTCCTTACATGTTGTTGAACTGCTCATCTAGCACGTGAGACATGCAACTTATCTCTAAAATCCTCCTCTCGTGTGGTTGTATGATCATTGTTATTGGTAAATGGTGACCCTGTGAATTGTGTACAGTTTCAATTTTAACACTGAATATAGTGACCTAATAATTTTCTTTTAATTTTTCATGTCATGCCTTAGACACATAGAGACTGTATTTTGGTGGTACAAAACTCCTGATGATTAGATCATATCCAAAAAGAAAATCTATTATTTTAATTAGTCCTTATTGGTCACACGAACTGCACTTATCAGAATCTTTCATTTTAGAATCAAGCTATAGTTATTTGATATAGGTTGACATTGTTTGTTCTGGGTTATGACTCAAAGCTCTCATCTCATTTTCTCAGGTGATGACTCAGAATTTACTCACTGGTAGCAGAAAGCGTGATTATCTAAGTTGTAGCACTGGGAAGTTTTTAGTATTACTTTTGATCCTCGGCTTGGGGTATCTTTTATTGACAAGTACTACTCCTGTCCATGTTCCCAATCGACTGGCCAAAGTTAGCGAGAATAATGAGAAAGGAGAGCTCAAGAATAATGGTGGCATCAACTTCAGGAACTTATGGAGGAGAGCTCCTAGGCTTCCTCCTCGACTACCACCAGATGAAAAAGGTAGCAATACTAGCTATCACATCGATCCAAACATGTTAATCTCTGAGTCATTATGGATTTCAAGGCAACAGAAAGTTAAGGATGCGTTTATCCATGCATGGTCTGGCTATAAAAGGTATGCAATGGGGTATGATGAACTTATGCCACTGAGCCAGCAAGGAACTGATGGGTTAGGAGGTTTAGGTGCTACGGTTGTGGATGCCCTTGACACTGCCATGATAATGGGTGCTAGTGATATTGTCTCTGAGGCAGGCTCATGGGTTGAAAAACACCTTCTGGATAGAATTAAGGAAAAGGGTCAAGTCAATTTATTTGAGACTACCATACGTGTTTTAGGTGGTCTCTTAAGTGCATATCACTTGAGAGCTGGAGAATATGGGAAGACCTTTGAAAATGAAGGACCTAAACCAGCTGTTTATCTAGAAATTGCAAAGAACTTGGCCGATCGTTTGTTAAGTGCTTTTACTTCCAGTCCAACCGCTATTCCTCTCTGTGATGTTGTTTTAAAAGATTCCTCAGCTCATGCAGCTCCTGGTGGATCAAGCAGCACATCCGAAGTATCTACTTTGCAGCTGGAATTCAATTATCTTAGTACTGTTTCAGGCGACCCAAAGTATTCAATTGCTGCTATGAAGGTCTTGGAACACATGAAGAACCTTCCGAAAGTGGAAGGTTTGGTTCCCATCTATATTAGGTATGGAGCTTTTATATCTAGCTATAAGCACGTTTACTTTATTTTAGACAATATGGTTTATTCTAACATGTGTGTCAAACTTTTTATGCCCTTTTTGTTATGATCTATGGACTCCTCTAGTTTTCATCTAGGTTGATAGCTTATCTCCCCACACCTTTTTTGGTATCCCTGACTTTCAGTATTTATGAATTACATACTGATGTGTGTGTGTAATCAATTCAATGAATATAACTGATATATATTTGACGTTGAACTTTCTATTTTCTTTTATTAATATATATTTTTGTCTTCTCATTGTGGTTAAATGAAAGGAAAATTATTATAAGTAGAAAAAAATGTTAAACTATTTACAAATATAGACAAATTTCACTGTCTATCGGCGATAGACCGTGATAAAAGTCTATCGCTCAAGTGATAGAAGTCTATCGGTCTATCGCTAATCGGCAGTAAAATTTTTCTATATTTGTAGATAGTTTGCCTCATTTTGCTATATTTGAAACCAACCCTAAATGAAATATTATTATTTTCAATGCAAAATTTTGGGCATCAAAATCATTCTGATCTGTCAAAATATACATATCTAAATAAAGAATCATAACAAATACTATCTTTGTCGATTATAATCGTGATGTTCTTTTAGCTCTCCTCCTGTTTTTTCTCCCTTTTTTCTAATATTCATACATTTCTTACAGTCACAAAATTGATTCTGTTCTTTTTTTATTTTAAAAAAATACATGTATATCTACTTACATAACAAATTCACAATATGCTAGATTGGTAGTCCTTTTAAAAGTTGATGTCTAGTCAGTAGTGTTATGTCCATACTTCACGGTGTGTGAGATATGGCTTCAATTAAACCTTTTGGCATGTTGGCAAAATTCTCTTTTCCAATCAAGAGAGGTTATTCTTCTCAGGCCAAGAGTGAAAGTAAATTGTTATGCTTGTATCAGTTTTATTTTCTTTTTTGGGGAACATATGGTCTTGTGGCTGTTTGTCCTTTTTGAGCACCAATCCTGAAAAGGTTCACTTTGTATTTCATATTTAATTTTCGAGTTTTGGTATATTGTGATTCCTTCTGCCACTCTAAATCTCCTGGTGCTTTTTGGTTCTTGAAAGGTTCCTTTAACTTAGAGGATATGGTGATGACTAAATTTACTTATTTTCACATAATTTTCTTCTCAATTACAGCCCTCATAGCGGCCAATTTAGTGGAGAGACCATAAGACTGGGATCTCGTGGTGATAGCTACTATGAGTATCTACTCAAAGTGTGGCTTCAGAAGCGAACGAGAGAAAATAATAATTTCACTTATCTGCATGATATGTATGAAGAAGCCATGAAAGGTGTTAAACATCTTCTTGTTCGGAAGTCTGTCCCAAAGGAGTTGGTTTTTGTTGGAGAATTGCCCTATGGATCTGGTTCTGCTATTAGTCCAAAAATGGACCACCTGGTATGCATTATTTCCTCCTGTATCTAACATTCACTTTTCTGGTAGACCAGCTTATGGTATAGTCTAGGCTATATATCAAGGAACATTGGCTCAAACTGAGCAATAATTTGGAAGATTGTTCCCCAAATTTGGGAGTTGCTTGATTTATCTGCATATTGATCCGTCTAAGGTATTCAACCTCGAAATGTCTCTGGATGTTTAGAAATATGATTTAAACTTTGAAAAGTAATATTACATATGATAAAGATGCTATATGTGCTCGTTGATTGACATAGAGCTATATACATAACTTCCATTCACCCTCAAGATCGGTATTAGTATAACAATGGAAACAGTTCCACTATCTTCAAGCCTGTTATGTATTTCTTCATGTGCAACAGACCTTGTGTTTGTGTTACAGATTTAGGATCTTACAAATTGTAAAAAACACAGGTTCACCTCCTTAAGTACGTTATGAAAATGTCTTTCTCCTCTGCTCTGTTATTGGCCGTTTCATCTGTTTCCAAGCAGTGAGACAAATTTTAAGCTTTAGAGCTTCTTTTCGTTTTATCTATACGGTAAAAAAGGAACAATAAGCGGACTTTGCTGCTAATCTGCTAGCCATTTACCAATATTTCCTCTTCAGCTTCTGTTGCCATTTATTCAAAGTATTGAACACTTCTCCGACATAACATTTAATAGCCTATGAGTTATTTGGCAACATAAATATATCTTCCTGTTCTCTTTAATGAATACAAAGAGATAAGGAGTAAATCTTGCGTTATCCTACATCAGTTGTGAAAAAGGATGGGGGTTGGTCTTCAAGTACTGAGGAAAAGTTTTGCCTCTTAACTTAGCTTTTGGGGAGAGAACCCCCATTTGGCACTAGCAAGTGCCATAAGACATTGGAAAGTATTAGGCAAAACCCACTTCATATTTTAGCTTAGGGAGAAAGTTTGTCTGGTTATGGGATTAGATGATGCAGGACAAAAGAAAACATACAAATAGTGGTAGAAGGGGGGGAATGTATGAGGAATTTGTATTTATTTACTTTTTTTTTTTTTTTAATAGTAATTAGTAAATAAAAACAAAAGTAACCGTTAGCTCATGTTCATATTGTTTTAAGGCTCTTTATTAGTGTAAATGTGTCCCCCTATAGTCTCTCTTGCATATTTTCATTCCAAGAGAATAGCAACGCCATTGTAATGGGTTCTAGATCAAGACTTCAAATTAATACTTTGATTCTTTAGCTCGTTGGTGATTAAAGCAATGTGACAGAATAAAGCATCACAGTAGCCCTTGTTTCGTTTTGGAAAAGTACTTTCACTAATAAACTTGTGCCTGTTTTAGGTATGTTTCTTATCAGGTACCCTTGCTCTTGGTGCCACCAAGGGCATCACCAAGGAAGAAGCAATGAGTAAAAGCTTGTTGAGCTTCGATGATCTGGAAAATCTAAATCTGGCAGAGGATCTTGCAAAAACTTGCTACGAGATGTATGAAGTAACCTCTACCGGTCTTGCCCCAGAAATAGTTTATTTCCATACCGAGGTCGGTTTGATCTATTGTAAATTTGTTCGCTATATCTTTCTTTCCCCATTTCTTCGTGCTGTCCATTAATCTAAATACTCTAGGTGCCTAATTTGTCCAGGAATATTCTGAACAAGGCTTCGATGGTGGAAACAAAAATTCAAAATACATTGATGACATAATAATCAAGCCATTGGATCGTCATAATCTGATGCGGCCCGAAACAGTCGAGTCATTGTTCGTCCTGTATCGTATCACAGGAAACCAAAAGTATGCCTTCTACATTAACCATGCTTCTTGATTGTTTATTACCGTGTTTCATATATTTGCATTGAATGAGGAAAAAAAAAAAGAACAAAAAAAGAAAGAAAGGATGTTTATATGAACGTACTGCAGGTACCGTGCATGGGGATGGGAGATTTTCGAAGCATTCGAGAGGCACACAAAGGTTAGTAGTGGTGGATATACATCCCTAGATGATGTTACTACAGTTCCTCCCCGGAGAAGGGACAAAATGGAGACATTCTTTCTGGGGGAGACTCTCAAGTATTTGTACTTGCTATTTGGAGACAGTTCCACGATTCCATTGGATAAATATGTGTTCAATACTGAAGCACATCCTCTACCAATTGAAGGCAATATTCAGATTAAATGA

mRNA sequence

ATGTCGAGTTCTCTACCCTACTCTGTCAAGGATGTTCATTACGACAATGCTAAGTTTCGACAGAGATCCTACTTCAAGGTGATGACTCAGAATTTACTCACTGGTAGCAGAAAGCGTGATTATCTAAGTTGTAGCACTGGGAAGTTTTTAGTATTACTTTTGATCCTCGGCTTGGGGTATCTTTTATTGACAAGTACTACTCCTGTCCATGTTCCCAATCGACTGGCCAAAGTTAGCGAGAATAATGAGAAAGGAGAGCTCAAGAATAATGGTGGCATCAACTTCAGGAACTTATGGAGGAGAGCTCCTAGGCTTCCTCCTCGACTACCACCAGATGAAAAAGGTAGCAATACTAGCTATCACATCGATCCAAACATGTTAATCTCTGAGTCATTATGGATTTCAAGGCAACAGAAAGTTAAGGATGCGTTTATCCATGCATGGTCTGGCTATAAAAGGTATGCAATGGGGTATGATGAACTTATGCCACTGAGCCAGCAAGGAACTGATGGGTTAGGAGGTTTAGGTGCTACGGTTGTGGATGCCCTTGACACTGCCATGATAATGGGTGCTAGTGATATTGTCTCTGAGGCAGGCTCATGGGTTGAAAAACACCTTCTGGATAGAATTAAGGAAAAGGGTCAAGTCAATTTATTTGAGACTACCATACGTGTTTTAGGTGGTCTCTTAAGTGCATATCACTTGAGAGCTGGAGAATATGGGAAGACCTTTGAAAATGAAGGACCTAAACCAGCTGTTTATCTAGAAATTGCAAAGAACTTGGCCGATCGTTTGTTAAGTGCTTTTACTTCCAGTCCAACCGCTATTCCTCTCTGTGATGTTGTTTTAAAAGATTCCTCAGCTCATGCAGCTCCTGGTGGATCAAGCAGCACATCCGAAGTATCTACTTTGCAGCTGGAATTCAATTATCTTAGTACTGTTTCAGGCGACCCAAAGTATTCAATTGCTGCTATGAAGGTCTTGGAACACATGAAGAACCTTCCGAAAGTGGAAGGTTTGGTTCCCATCTATATTAGCCCTCATAGCGGCCAATTTAGTGGAGAGACCATAAGACTGGGATCTCGTGGTGATAGCTACTATGAGTATCTACTCAAAGTGTGGCTTCAGAAGCGAACGAGAGAAAATAATAATTTCACTTATCTGCATGATATGTATGAAGAAGCCATGAAAGGTGTTAAACATCTTCTTGTTCGGAAGTCTGTCCCAAAGGAGTTGGTTTTTGTTGGAGAATTGCCCTATGGATCTGGTTCTGCTATTAGTCCAAAAATGGACCACCTGGTATGTTTCTTATCAGGTACCCTTGCTCTTGGTGCCACCAAGGGCATCACCAAGGAAGAAGCAATGAGTAAAAGCTTGTTGAGCTTCGATGATCTGGAAAATCTAAATCTGGCAGAGGATCTTGCAAAAACTTGCTACGAGATGTATGAAGTAACCTCTACCGGTCTTGCCCCAGAAATAGTTTATTTCCATACCGAGGAATATTCTGAACAAGGCTTCGATGGTGGAAACAAAAATTCAAAATACATTGATGACATAATAATCAAGCCATTGGATCGTCATAATCTGATGCGGCCCGAAACAGTCGAGTCATTGTTCGTCCTGTATCGTATCACAGGAAACCAAAAGTACCGTGCATGGGGATGGGAGATTTTCGAAGCATTCGAGAGGCACACAAAGGTTAGTAGTGGTGGATATACATCCCTAGATGATGTTACTACAGTTCCTCCCCGGAGAAGGGACAAAATGGAGACATTCTTTCTGGGGGAGACTCTCAAGTATTTGTACTTGCTATTTGGAGACAGTTCCACGATTCCATTGGATAAATATGTGTTCAATACTGAAGCACATCCTCTACCAATTGAAGGCAATATTCAGATTAAATGA

Coding sequence (CDS)

ATGTCGAGTTCTCTACCCTACTCTGTCAAGGATGTTCATTACGACAATGCTAAGTTTCGACAGAGATCCTACTTCAAGGTGATGACTCAGAATTTACTCACTGGTAGCAGAAAGCGTGATTATCTAAGTTGTAGCACTGGGAAGTTTTTAGTATTACTTTTGATCCTCGGCTTGGGGTATCTTTTATTGACAAGTACTACTCCTGTCCATGTTCCCAATCGACTGGCCAAAGTTAGCGAGAATAATGAGAAAGGAGAGCTCAAGAATAATGGTGGCATCAACTTCAGGAACTTATGGAGGAGAGCTCCTAGGCTTCCTCCTCGACTACCACCAGATGAAAAAGGTAGCAATACTAGCTATCACATCGATCCAAACATGTTAATCTCTGAGTCATTATGGATTTCAAGGCAACAGAAAGTTAAGGATGCGTTTATCCATGCATGGTCTGGCTATAAAAGGTATGCAATGGGGTATGATGAACTTATGCCACTGAGCCAGCAAGGAACTGATGGGTTAGGAGGTTTAGGTGCTACGGTTGTGGATGCCCTTGACACTGCCATGATAATGGGTGCTAGTGATATTGTCTCTGAGGCAGGCTCATGGGTTGAAAAACACCTTCTGGATAGAATTAAGGAAAAGGGTCAAGTCAATTTATTTGAGACTACCATACGTGTTTTAGGTGGTCTCTTAAGTGCATATCACTTGAGAGCTGGAGAATATGGGAAGACCTTTGAAAATGAAGGACCTAAACCAGCTGTTTATCTAGAAATTGCAAAGAACTTGGCCGATCGTTTGTTAAGTGCTTTTACTTCCAGTCCAACCGCTATTCCTCTCTGTGATGTTGTTTTAAAAGATTCCTCAGCTCATGCAGCTCCTGGTGGATCAAGCAGCACATCCGAAGTATCTACTTTGCAGCTGGAATTCAATTATCTTAGTACTGTTTCAGGCGACCCAAAGTATTCAATTGCTGCTATGAAGGTCTTGGAACACATGAAGAACCTTCCGAAAGTGGAAGGTTTGGTTCCCATCTATATTAGCCCTCATAGCGGCCAATTTAGTGGAGAGACCATAAGACTGGGATCTCGTGGTGATAGCTACTATGAGTATCTACTCAAAGTGTGGCTTCAGAAGCGAACGAGAGAAAATAATAATTTCACTTATCTGCATGATATGTATGAAGAAGCCATGAAAGGTGTTAAACATCTTCTTGTTCGGAAGTCTGTCCCAAAGGAGTTGGTTTTTGTTGGAGAATTGCCCTATGGATCTGGTTCTGCTATTAGTCCAAAAATGGACCACCTGGTATGTTTCTTATCAGGTACCCTTGCTCTTGGTGCCACCAAGGGCATCACCAAGGAAGAAGCAATGAGTAAAAGCTTGTTGAGCTTCGATGATCTGGAAAATCTAAATCTGGCAGAGGATCTTGCAAAAACTTGCTACGAGATGTATGAAGTAACCTCTACCGGTCTTGCCCCAGAAATAGTTTATTTCCATACCGAGGAATATTCTGAACAAGGCTTCGATGGTGGAAACAAAAATTCAAAATACATTGATGACATAATAATCAAGCCATTGGATCGTCATAATCTGATGCGGCCCGAAACAGTCGAGTCATTGTTCGTCCTGTATCGTATCACAGGAAACCAAAAGTACCGTGCATGGGGATGGGAGATTTTCGAAGCATTCGAGAGGCACACAAAGGTTAGTAGTGGTGGATATACATCCCTAGATGATGTTACTACAGTTCCTCCCCGGAGAAGGGACAAAATGGAGACATTCTTTCTGGGGGAGACTCTCAAGTATTTGTACTTGCTATTTGGAGACAGTTCCACGATTCCATTGGATAAATATGTGTTCAATACTGAAGCACATCCTCTACCAATTGAAGGCAATATTCAGATTAAATGA

Protein sequence

MSSSLPYSVKDVHYDNAKFRQRSYFKVMTQNLLTGSRKRDYLSCSTGKFLVLLLILGLGYLLLTSTTPVHVPNRLAKVSENNEKGELKNNGGINFRNLWRRAPRLPPRLPPDEKGSNTSYHIDPNMLISESLWISRQQKVKDAFIHAWSGYKRYAMGYDELMPLSQQGTDGLGGLGATVVDALDTAMIMGASDIVSEAGSWVEKHLLDRIKEKGQVNLFETTIRVLGGLLSAYHLRAGEYGKTFENEGPKPAVYLEIAKNLADRLLSAFTSSPTAIPLCDVVLKDSSAHAAPGGSSSTSEVSTLQLEFNYLSTVSGDPKYSIAAMKVLEHMKNLPKVEGLVPIYISPHSGQFSGETIRLGSRGDSYYEYLLKVWLQKRTRENNNFTYLHDMYEEAMKGVKHLLVRKSVPKELVFVGELPYGSGSAISPKMDHLVCFLSGTLALGATKGITKEEAMSKSLLSFDDLENLNLAEDLAKTCYEMYEVTSTGLAPEIVYFHTEEYSEQGFDGGNKNSKYIDDIIIKPLDRHNLMRPETVESLFVLYRITGNQKYRAWGWEIFEAFERHTKVSSGGYTSLDDVTTVPPRRRDKMETFFLGETLKYLYLLFGDSSTIPLDKYVFNTEAHPLPIEGNIQIK
Homology
BLAST of Cla97C01G024530 vs. NCBI nr
Match: XP_038882608.1 (mannosyl-oligosaccharide 1,2-alpha-mannosidase MNS3 isoform X1 [Benincasa hispida])

HSP 1 Score: 1234.6 bits (3193), Expect = 0.0e+00
Identity = 613/634 (96.69%), Postives = 617/634 (97.32%), Query Frame = 0

Query: 1   MSSSLPYSVKDVHYDNAKFRQRSYFKVMTQNLLTGSRKRDYLSCSTGKFLVLLLILGLGY 60
           MSSSLPYSVKDVHYDNAKFRQRSYFKVMTQNLLTGSRKRDYLSCSTGKFLVLLLI GL Y
Sbjct: 1   MSSSLPYSVKDVHYDNAKFRQRSYFKVMTQNLLTGSRKRDYLSCSTGKFLVLLLIFGLAY 60

Query: 61  LLLTSTTPVHVPNRLAKVSENNEKGELKNNGGINFRNLWRRAPRLPPRLPPDEKGSNTSY 120
           LLLTSTTPVH PNR+AKV ENNEKGE KNNGGINFRNLWRRAPRLPPRLPPDEKGSNTSY
Sbjct: 61  LLLTSTTPVHGPNRVAKVGENNEKGEFKNNGGINFRNLWRRAPRLPPRLPPDEKGSNTSY 120

Query: 121 HIDPNMLISESLWISRQQKVKDAFIHAWSGYKRYAMGYDELMPLSQQGTDGLGGLGATVV 180
           H DPN LISESLWISRQQKVKDAFIHAWSGYKRYAMGYDELMPLSQ GTDGLGGLGATVV
Sbjct: 121 HFDPNTLISESLWISRQQKVKDAFIHAWSGYKRYAMGYDELMPLSQHGTDGLGGLGATVV 180

Query: 181 DALDTAMIMGASDIVSEAGSWVEKHLLDRIKEKGQVNLFETTIRVLGGLLSAYHLRAGEY 240
           DALDTAMIMGASDIVSEAGSWVEKHLLDRIKEKGQVNLFETTIRVLGGLLSAYHLRAGEY
Sbjct: 181 DALDTAMIMGASDIVSEAGSWVEKHLLDRIKEKGQVNLFETTIRVLGGLLSAYHLRAGEY 240

Query: 241 GKTFENEGPKPAVYLEIAKNLADRLLSAFTSSPTAIPLCDVVLKDSSAHAAPGGSSSTSE 300
           GK FENEGPKPAVYLEIAKNLADRLLSAFTSSPTAIPLCDVVLKDSSAHAAPGGSSSTSE
Sbjct: 241 GKRFENEGPKPAVYLEIAKNLADRLLSAFTSSPTAIPLCDVVLKDSSAHAAPGGSSSTSE 300

Query: 301 VSTLQLEFNYLSTVSGDPKYSIAAMKVLEHMKNLPKVEGLVPIYISPHSGQFSGETIRLG 360
           VSTLQLEFNYLSTVSGDPKYSIAAMKVLEHMK+LPKVEGLVPIYISP +GQFSGETIRLG
Sbjct: 301 VSTLQLEFNYLSTVSGDPKYSIAAMKVLEHMKSLPKVEGLVPIYISPQTGQFSGETIRLG 360

Query: 361 SRGDSYYEYLLKVWLQKRTRENNNFTYLHDMYEEAMKGVKHLLVRKSVPKELVFVGELPY 420
           SRGDSYYEYLLKVWLQKR RENNNFTYLHDMYEEAMKGVKHLLVRKSVP  LVFVGELPY
Sbjct: 361 SRGDSYYEYLLKVWLQKRRRENNNFTYLHDMYEEAMKGVKHLLVRKSVPNGLVFVGELPY 420

Query: 421 GSGSAISPKMDHLVCFLSGTLALGATKGITKEEAMSKSLLSFDDLENLNLAEDLAKTCYE 480
           GS S+ISPKMDHLVCFLSGTLALGATKGITKEEAMSKSLLSFDDLENLNLAEDLAKTCYE
Sbjct: 421 GSDSSISPKMDHLVCFLSGTLALGATKGITKEEAMSKSLLSFDDLENLNLAEDLAKTCYE 480

Query: 481 MYEVTSTGLAPEIVYFHTEEYSEQGFDGGNKNSKYIDDIIIKPLDRHNLMRPETVESLFV 540
           MYEVTSTGLAPEIVYFHTEEYSEQG DGGNKNSKYI DIIIKPLDRHNLMRPETVESLFV
Sbjct: 481 MYEVTSTGLAPEIVYFHTEEYSEQGLDGGNKNSKYIGDIIIKPLDRHNLMRPETVESLFV 540

Query: 541 LYRITGNQKYRAWGWEIFEAFERHTKVSSGGYTSLDDVTTVPPRRRDKMETFFLGETLKY 600
           LYRITGN KYRAWGWEIFEAFERHTKVSSGGYTSLDDVTTVPPRRRDKMETFFLGETLKY
Sbjct: 541 LYRITGNPKYRAWGWEIFEAFERHTKVSSGGYTSLDDVTTVPPRRRDKMETFFLGETLKY 600

Query: 601 LYLLFGDSSTIPLDKYVFNTEAHPLPIEGNIQIK 635
           LYLLFGDSSTIPLDKYVFNTEAHPLPIEGNIQIK
Sbjct: 601 LYLLFGDSSTIPLDKYVFNTEAHPLPIEGNIQIK 634

BLAST of Cla97C01G024530 vs. NCBI nr
Match: XP_008439639.1 (PREDICTED: mannosyl-oligosaccharide 1,2-alpha-mannosidase MNS3 [Cucumis melo])

HSP 1 Score: 1219.5 bits (3154), Expect = 0.0e+00
Identity = 600/634 (94.64%), Postives = 617/634 (97.32%), Query Frame = 0

Query: 1   MSSSLPYSVKDVHYDNAKFRQRSYFKVMTQNLLTGSRKRDYLSCSTGKFLVLLLILGLGY 60
           MSSSLPYSVKDVHYDNAKFRQRSY KVMTQNLLTGSRKRDYLSCSTGKFL+LL+I GL Y
Sbjct: 1   MSSSLPYSVKDVHYDNAKFRQRSYLKVMTQNLLTGSRKRDYLSCSTGKFLILLMIFGLAY 60

Query: 61  LLLTSTTPVHVPNRLAKVSENNEKGELKNNGGINFRNLWRRAPRLPPRLPPDEKGSNTSY 120
           LLLT+ TPVHVPN+ AKVSENNEK E+KNNGGINFRNLWR+APRLPP+LPPDEKGSNTSY
Sbjct: 61  LLLTNATPVHVPNQPAKVSENNEKREIKNNGGINFRNLWRKAPRLPPQLPPDEKGSNTSY 120

Query: 121 HIDPNMLISESLWISRQQKVKDAFIHAWSGYKRYAMGYDELMPLSQQGTDGLGGLGATVV 180
           HIDPN LISESLWISRQQKVKDAFIHAWSGYKRYAMGYDELMPLSQ GTDGLGGLGATVV
Sbjct: 121 HIDPNTLISESLWISRQQKVKDAFIHAWSGYKRYAMGYDELMPLSQHGTDGLGGLGATVV 180

Query: 181 DALDTAMIMGASDIVSEAGSWVEKHLLDRIKEKGQVNLFETTIRVLGGLLSAYHLRAGEY 240
           DALDTAMIMGASDIVSEAGSW+EKHL+DRIKEKGQVNLFETTIRV+GGLLSAYHLRAGEY
Sbjct: 181 DALDTAMIMGASDIVSEAGSWIEKHLMDRIKEKGQVNLFETTIRVVGGLLSAYHLRAGEY 240

Query: 241 GKTFENEGPKPAVYLEIAKNLADRLLSAFTSSPTAIPLCDVVLKDSSAHAAPGGSSSTSE 300
           GKTF NEGPKPAVYLEIAKNLADRLLSAFTSSPTAIPLCDVVLKDSSAHAAPGGSSSTSE
Sbjct: 241 GKTFGNEGPKPAVYLEIAKNLADRLLSAFTSSPTAIPLCDVVLKDSSAHAAPGGSSSTSE 300

Query: 301 VSTLQLEFNYLSTVSGDPKYSIAAMKVLEHMKNLPKVEGLVPIYISPHSGQFSGETIRLG 360
           VSTLQLEFNYLSTVSGDPKYSIAAMKVLEHMK+LPKVEGLVPIYISPH+G+FSGETIRLG
Sbjct: 301 VSTLQLEFNYLSTVSGDPKYSIAAMKVLEHMKSLPKVEGLVPIYISPHTGEFSGETIRLG 360

Query: 361 SRGDSYYEYLLKVWLQKRTRENNNFTYLHDMYEEAMKGVKHLLVRKSVPKELVFVGELPY 420
           SRGDSYYEYLLKVWLQKRT  NNNFTYLHDMYEEAMKGVKHLLVRKS+P  LVFVGELPY
Sbjct: 361 SRGDSYYEYLLKVWLQKRTSGNNNFTYLHDMYEEAMKGVKHLLVRKSIPNGLVFVGELPY 420

Query: 421 GSGSAISPKMDHLVCFLSGTLALGATKGITKEEAMSKSLLSFDDLENLNLAEDLAKTCYE 480
           GS SAISPKMDHLVCFLSGTLALGATKGITKEEAMSKSLLSFDDLENLNLAEDLAKTC+E
Sbjct: 421 GSDSAISPKMDHLVCFLSGTLALGATKGITKEEAMSKSLLSFDDLENLNLAEDLAKTCFE 480

Query: 481 MYEVTSTGLAPEIVYFHTEEYSEQGFDGGNKNSKYIDDIIIKPLDRHNLMRPETVESLFV 540
           MYEVTSTGLAPEI YFHTEEYSEQG DGGNKNSKYIDDIIIKPLDRHNLMRPETVESLFV
Sbjct: 481 MYEVTSTGLAPEIAYFHTEEYSEQGHDGGNKNSKYIDDIIIKPLDRHNLMRPETVESLFV 540

Query: 541 LYRITGNQKYRAWGWEIFEAFERHTKVSSGGYTSLDDVTTVPPRRRDKMETFFLGETLKY 600
           LYRITGN KYRAWGWEIFEAFERHTKVSSGGYTSLDDVTTVPPRRRDKMETFFLGETLKY
Sbjct: 541 LYRITGNPKYRAWGWEIFEAFERHTKVSSGGYTSLDDVTTVPPRRRDKMETFFLGETLKY 600

Query: 601 LYLLFGDSSTIPLDKYVFNTEAHPLPIEGNIQIK 635
           LYLLFGDSSTIPLDKYVFNTEAHPLP+EGNIQI+
Sbjct: 601 LYLLFGDSSTIPLDKYVFNTEAHPLPMEGNIQIQ 634

BLAST of Cla97C01G024530 vs. NCBI nr
Match: XP_004134582.1 (mannosyl-oligosaccharide 1,2-alpha-mannosidase MNS3 [Cucumis sativus] >KGN49392.1 hypothetical protein Csa_002711 [Cucumis sativus])

HSP 1 Score: 1209.1 bits (3127), Expect = 0.0e+00
Identity = 594/634 (93.69%), Postives = 616/634 (97.16%), Query Frame = 0

Query: 1   MSSSLPYSVKDVHYDNAKFRQRSYFKVMTQNLLTGSRKRDYLSCSTGKFLVLLLILGLGY 60
           MSSSLPYSVKDVHYDNAKFRQRSY KVMTQNLLTGSRKRDYLSCSTGKFLVLLLI GL Y
Sbjct: 1   MSSSLPYSVKDVHYDNAKFRQRSYVKVMTQNLLTGSRKRDYLSCSTGKFLVLLLIFGLAY 60

Query: 61  LLLTSTTPVHVPNRLAKVSENNEKGELKNNGGINFRNLWRRAPRLPPRLPPDEKGSNTSY 120
           LLLTS TPVHVPN+LAKVSEN+EK E+KNNGGINFRNLWR+APRLPP+LPPDEKGSNTSY
Sbjct: 61  LLLTSATPVHVPNKLAKVSENSEKQEIKNNGGINFRNLWRKAPRLPPQLPPDEKGSNTSY 120

Query: 121 HIDPNMLISESLWISRQQKVKDAFIHAWSGYKRYAMGYDELMPLSQQGTDGLGGLGATVV 180
           HIDPN LISESLWISRQQKVKDAFIHAWSGYKRYAMGYDELMPLSQ GTDGLGGLGATVV
Sbjct: 121 HIDPNTLISESLWISRQQKVKDAFIHAWSGYKRYAMGYDELMPLSQHGTDGLGGLGATVV 180

Query: 181 DALDTAMIMGASDIVSEAGSWVEKHLLDRIKEKGQVNLFETTIRVLGGLLSAYHLRAGEY 240
           DALDTAMIMGASDIVSEAGSW+EK+L+DRIKEKGQVNLFETTIR++GGLLSAYHLRAGEY
Sbjct: 181 DALDTAMIMGASDIVSEAGSWIEKNLMDRIKEKGQVNLFETTIRIVGGLLSAYHLRAGEY 240

Query: 241 GKTFENEGPKPAVYLEIAKNLADRLLSAFTSSPTAIPLCDVVLKDSSAHAAPGGSSSTSE 300
           GKTF NEGPKPAVYLEIAKNLADRLLSAFTSSPTAIPLCDVVLKDSSAHAAPGGSSSTSE
Sbjct: 241 GKTFGNEGPKPAVYLEIAKNLADRLLSAFTSSPTAIPLCDVVLKDSSAHAAPGGSSSTSE 300

Query: 301 VSTLQLEFNYLSTVSGDPKYSIAAMKVLEHMKNLPKVEGLVPIYISPHSGQFSGETIRLG 360
           VSTLQLEFNYLSTVSGDPKYSIAAMKVLEHMK+LPKVEGLVPIYISPH+G+FSGETIRLG
Sbjct: 301 VSTLQLEFNYLSTVSGDPKYSIAAMKVLEHMKSLPKVEGLVPIYISPHTGKFSGETIRLG 360

Query: 361 SRGDSYYEYLLKVWLQKRTRENNNFTYLHDMYEEAMKGVKHLLVRKSVPKELVFVGELPY 420
           SRGDSYYEYLLKVWLQKRT  N+NFTYLHDMYEEAMKGVKHLLVRKS+P  LVFVGELPY
Sbjct: 361 SRGDSYYEYLLKVWLQKRTSGNSNFTYLHDMYEEAMKGVKHLLVRKSIPNGLVFVGELPY 420

Query: 421 GSGSAISPKMDHLVCFLSGTLALGATKGITKEEAMSKSLLSFDDLENLNLAEDLAKTCYE 480
           GS S ISPKMDHLVCFLSGTLALGATKGITKEEAMSKSLL+FDDLENLNLAEDLAKTC+E
Sbjct: 421 GSDSTISPKMDHLVCFLSGTLALGATKGITKEEAMSKSLLNFDDLENLNLAEDLAKTCFE 480

Query: 481 MYEVTSTGLAPEIVYFHTEEYSEQGFDGGNKNSKYIDDIIIKPLDRHNLMRPETVESLFV 540
           MYEVTSTGLAPEI YFHTEEYSEQG DGGNK SKYIDDIIIKPLDRHNLMRPETVESLFV
Sbjct: 481 MYEVTSTGLAPEIAYFHTEEYSEQGLDGGNKKSKYIDDIIIKPLDRHNLMRPETVESLFV 540

Query: 541 LYRITGNQKYRAWGWEIFEAFERHTKVSSGGYTSLDDVTTVPPRRRDKMETFFLGETLKY 600
           LYRITG+ KYRAWGWEIFEAFERHTKVSSGGYTSLDDVTT+PPRRRDKMETFFLGETLKY
Sbjct: 541 LYRITGDPKYRAWGWEIFEAFERHTKVSSGGYTSLDDVTTIPPRRRDKMETFFLGETLKY 600

Query: 601 LYLLFGDSSTIPLDKYVFNTEAHPLPIEGNIQIK 635
           LYLLFGDSSTIPLDKYVFNTEAHPLP++GNIQI+
Sbjct: 601 LYLLFGDSSTIPLDKYVFNTEAHPLPMDGNIQIQ 634

BLAST of Cla97C01G024530 vs. NCBI nr
Match: XP_038882609.1 (mannosyl-oligosaccharide 1,2-alpha-mannosidase MNS3 isoform X2 [Benincasa hispida])

HSP 1 Score: 1182.5 bits (3058), Expect = 0.0e+00
Identity = 587/608 (96.55%), Postives = 591/608 (97.20%), Query Frame = 0

Query: 27  VMTQNLLTGSRKRDYLSCSTGKFLVLLLILGLGYLLLTSTTPVHVPNRLAKVSENNEKGE 86
           VMTQNLLTGSRKRDYLSCSTGKFLVLLLI GL YLLLTSTTPVH PNR+AKV ENNEKGE
Sbjct: 20  VMTQNLLTGSRKRDYLSCSTGKFLVLLLIFGLAYLLLTSTTPVHGPNRVAKVGENNEKGE 79

Query: 87  LKNNGGINFRNLWRRAPRLPPRLPPDEKGSNTSYHIDPNMLISESLWISRQQKVKDAFIH 146
            KNNGGINFRNLWRRAPRLPPRLPPDEKGSNTSYH DPN LISESLWISRQQKVKDAFIH
Sbjct: 80  FKNNGGINFRNLWRRAPRLPPRLPPDEKGSNTSYHFDPNTLISESLWISRQQKVKDAFIH 139

Query: 147 AWSGYKRYAMGYDELMPLSQQGTDGLGGLGATVVDALDTAMIMGASDIVSEAGSWVEKHL 206
           AWSGYKRYAMGYDELMPLSQ GTDGLGGLGATVVDALDTAMIMGASDIVSEAGSWVEKHL
Sbjct: 140 AWSGYKRYAMGYDELMPLSQHGTDGLGGLGATVVDALDTAMIMGASDIVSEAGSWVEKHL 199

Query: 207 LDRIKEKGQVNLFETTIRVLGGLLSAYHLRAGEYGKTFENEGPKPAVYLEIAKNLADRLL 266
           LDRIKEKGQVNLFETTIRVLGGLLSAYHLRAGEYGK FENEGPKPAVYLEIAKNLADRLL
Sbjct: 200 LDRIKEKGQVNLFETTIRVLGGLLSAYHLRAGEYGKRFENEGPKPAVYLEIAKNLADRLL 259

Query: 267 SAFTSSPTAIPLCDVVLKDSSAHAAPGGSSSTSEVSTLQLEFNYLSTVSGDPKYSIAAMK 326
           SAFTSSPTAIPLCDVVLKDSSAHAAPGGSSSTSEVSTLQLEFNYLSTVSGDPKYSIAAMK
Sbjct: 260 SAFTSSPTAIPLCDVVLKDSSAHAAPGGSSSTSEVSTLQLEFNYLSTVSGDPKYSIAAMK 319

Query: 327 VLEHMKNLPKVEGLVPIYISPHSGQFSGETIRLGSRGDSYYEYLLKVWLQKRTRENNNFT 386
           VLEHMK+LPKVEGLVPIYISP +GQFSGETIRLGSRGDSYYEYLLKVWLQKR RENNNFT
Sbjct: 320 VLEHMKSLPKVEGLVPIYISPQTGQFSGETIRLGSRGDSYYEYLLKVWLQKRRRENNNFT 379

Query: 387 YLHDMYEEAMKGVKHLLVRKSVPKELVFVGELPYGSGSAISPKMDHLVCFLSGTLALGAT 446
           YLHDMYEEAMKGVKHLLVRKSVP  LVFVGELPYGS S+ISPKMDHLVCFLSGTLALGAT
Sbjct: 380 YLHDMYEEAMKGVKHLLVRKSVPNGLVFVGELPYGSDSSISPKMDHLVCFLSGTLALGAT 439

Query: 447 KGITKEEAMSKSLLSFDDLENLNLAEDLAKTCYEMYEVTSTGLAPEIVYFHTEEYSEQGF 506
           KGITKEEAMSKSLLSFDDLENLNLAEDLAKTCYEMYEVTSTGLAPEIVYFHTEEYSEQG 
Sbjct: 440 KGITKEEAMSKSLLSFDDLENLNLAEDLAKTCYEMYEVTSTGLAPEIVYFHTEEYSEQGL 499

Query: 507 DGGNKNSKYIDDIIIKPLDRHNLMRPETVESLFVLYRITGNQKYRAWGWEIFEAFERHTK 566
           DGGNKNSKYI DIIIKPLDRHNLMRPETVESLFVLYRITGN KYRAWGWEIFEAFERHTK
Sbjct: 500 DGGNKNSKYIGDIIIKPLDRHNLMRPETVESLFVLYRITGNPKYRAWGWEIFEAFERHTK 559

Query: 567 VSSGGYTSLDDVTTVPPRRRDKMETFFLGETLKYLYLLFGDSSTIPLDKYVFNTEAHPLP 626
           VSSGGYTSLDDVTTVPPRRRDKMETFFLGETLKYLYLLFGDSSTIPLDKYVFNTEAHPLP
Sbjct: 560 VSSGGYTSLDDVTTVPPRRRDKMETFFLGETLKYLYLLFGDSSTIPLDKYVFNTEAHPLP 619

Query: 627 IEGNIQIK 635
           IEGNIQIK
Sbjct: 620 IEGNIQIK 627

BLAST of Cla97C01G024530 vs. NCBI nr
Match: XP_023517941.1 (mannosyl-oligosaccharide 1,2-alpha-mannosidase MNS3-like [Cucurbita pepo subsp. pepo] >XP_023517942.1 mannosyl-oligosaccharide 1,2-alpha-mannosidase MNS3-like [Cucurbita pepo subsp. pepo] >XP_023517943.1 mannosyl-oligosaccharide 1,2-alpha-mannosidase MNS3-like [Cucurbita pepo subsp. pepo])

HSP 1 Score: 1181.0 bits (3054), Expect = 0.0e+00
Identity = 585/633 (92.42%), Postives = 606/633 (95.73%), Query Frame = 0

Query: 1   MSSSLPYSVKDVHYDNAKFRQRSYFKVMTQNLLTGSRKRDYLSCSTGKFLVLLLILGLGY 60
           M SSLPYSVKDVHYDNAKFRQRSYFKVMTQNLLTGSRKRDYLSCSTGKFLVLLLI GL Y
Sbjct: 1   MRSSLPYSVKDVHYDNAKFRQRSYFKVMTQNLLTGSRKRDYLSCSTGKFLVLLLIFGLAY 60

Query: 61  LLLTSTTP-VHVPNRLAKVSENNEKGELKNNGGINFRNLWRRAPRLPPRLPPDEKGSNTS 120
           LLLTSTTP   VPNRL KVSENN+KGE K+NGGINFRNLWR+ PRLPPRLPPDEKGSNTS
Sbjct: 61  LLLTSTTPGDFVPNRLDKVSENNQKGEFKSNGGINFRNLWRKPPRLPPRLPPDEKGSNTS 120

Query: 121 YHIDPNMLISESLWISRQQKVKDAFIHAWSGYKRYAMGYDELMPLSQQGTDGLGGLGATV 180
           YHIDPN LISESLWISRQQKVKDAFIHAWSGYKRYAMG+DELMPLSQQGTDGLGGLGATV
Sbjct: 121 YHIDPNTLISESLWISRQQKVKDAFIHAWSGYKRYAMGHDELMPLSQQGTDGLGGLGATV 180

Query: 181 VDALDTAMIMGASDIVSEAGSWVEKHLLDRIKEKGQVNLFETTIRVLGGLLSAYHLRAGE 240
           VDALDTAMIMGASDIVSEAGSWVEKHLLDRIKEKGQVNLFETTIRVLGGLLSAYHLRAGE
Sbjct: 181 VDALDTAMIMGASDIVSEAGSWVEKHLLDRIKEKGQVNLFETTIRVLGGLLSAYHLRAGE 240

Query: 241 YGKTFENEGPKPAVYLEIAKNLADRLLSAFTSSPTAIPLCDVVLKDSSAHAAPGGSSSTS 300
           YGKTFENEGPKPAVYLE AKNLADRLLSAFTSSPTAIPLCDVVL+DSSAHAAPGG SSTS
Sbjct: 241 YGKTFENEGPKPAVYLETAKNLADRLLSAFTSSPTAIPLCDVVLRDSSAHAAPGGLSSTS 300

Query: 301 EVSTLQLEFNYLSTVSGDPKYSIAAMKVLEHMKNLPKVEGLVPIYISPHSGQFSGETIRL 360
           EVSTLQLEFNYLST+SGDPKYS+AAMKVLEHMK+LPK+EGLVPIYISP SGQFSGETIRL
Sbjct: 301 EVSTLQLEFNYLSTISGDPKYSMAAMKVLEHMKSLPKLEGLVPIYISPDSGQFSGETIRL 360

Query: 361 GSRGDSYYEYLLKVWLQKRTRENNNFTYLHDMYEEAMKGVKHLLVRKSVPKELVFVGELP 420
           GSRGDSYYEYLLKVWLQK  REN+N TYLHDMYEEAMKGVKHLLVRKSVP  LVFVGELP
Sbjct: 361 GSRGDSYYEYLLKVWLQKGARENHNLTYLHDMYEEAMKGVKHLLVRKSVPNGLVFVGELP 420

Query: 421 YGSGSAISPKMDHLVCFLSGTLALGATKGITKEEAMSKSLLSFDDLENLNLAEDLAKTCY 480
           YGS  AISPKMDHLVCFL GTLALGATKGI+KEEAM KSLLSF+DLENLNLAEDLAKTC+
Sbjct: 421 YGSSEAISPKMDHLVCFLPGTLALGATKGISKEEAMKKSLLSFEDLENLNLAEDLAKTCF 480

Query: 481 EMYEVTSTGLAPEIVYFHTEEYSEQGFDGGNKNSKYIDDIIIKPLDRHNLMRPETVESLF 540
           EMYEVT+TGLAPEIVYFHTEEYSE G DGGNKN+KYIDDIIIKP DRHNL+RPETVESLF
Sbjct: 481 EMYEVTATGLAPEIVYFHTEEYSEGGHDGGNKNAKYIDDIIIKPPDRHNLLRPETVESLF 540

Query: 541 VLYRITGNQKYRAWGWEIFEAFERHTKVSSGGYTSLDDVTTVPPRRRDKMETFFLGETLK 600
           VLYRITG+ KYRAWGW+IFEAFERHTKV++GGYTSLDDVTTVPPRRRDKMETFFLGETLK
Sbjct: 541 VLYRITGDPKYRAWGWQIFEAFERHTKVNTGGYTSLDDVTTVPPRRRDKMETFFLGETLK 600

Query: 601 YLYLLFGDSSTIPLDKYVFNTEAHPLPIEGNIQ 633
           YLYLLFGD+STIPLDKYVFNTEAHPLPIE NIQ
Sbjct: 601 YLYLLFGDNSTIPLDKYVFNTEAHPLPIETNIQ 633

BLAST of Cla97C01G024530 vs. ExPASy Swiss-Prot
Match: Q93Y37 (Mannosyl-oligosaccharide 1,2-alpha-mannosidase MNS3 OS=Arabidopsis thaliana OX=3702 GN=MNS3 PE=1 SV=1)

HSP 1 Score: 914.1 bits (2361), Expect = 8.9e-265
Identity = 455/634 (71.77%), Postives = 523/634 (82.49%), Query Frame = 0

Query: 1   MSSSLPYSVKDVHYDNAKFRQRSYFKVMTQNLLTGSRKRDYLSCSTGKFLVLLLILGLGY 60
           MS SLPYSVKD+HYDNAKFR RS  KV +Q+LLT S KR+Y SCSTGKFL+L+L  G+  
Sbjct: 1   MSKSLPYSVKDIHYDNAKFRHRSPLKVFSQSLLTLSTKRNYASCSTGKFLILILFFGVAC 60

Query: 61  LLLTSTTPVHVPNRLAKVSENNEKGELKNNGGINFRNLWRRAPRLPPRLPPDE---KGSN 120
           L+L S +P          S  NEKG++   GG+    L R+ PRLPPRL PDE   +GS+
Sbjct: 61  LMLMSKSP--------NESGLNEKGKVTFVGGLRLGGLLRKPPRLPPRLSPDEGQLRGSS 120

Query: 121 TSYHIDPNMLISESLWISRQQKVKDAFIHAWSGYKRYAMGYDELMPLSQQGTDGLGGLGA 180
           T+     N   S+  W +RQQ VK+AF HAWSGY++YAMGYDELMP+SQ+G DGLGGLGA
Sbjct: 121 TNGSTISN---SDPKWAARQQSVKEAFDHAWSGYRKYAMGYDELMPISQKGVDGLGGLGA 180

Query: 181 TVVDALDTAMIMGASDIVSEAGSWVEKHLLDRIKEKGQVNLFETTIRVLGGLLSAYHLRA 240
           TVVDALDTAMIMG  +IVSEAGSWVE HLL+RI +KGQVNLFETTIRVLGGLLSAYHL  
Sbjct: 181 TVVDALDTAMIMGLDNIVSEAGSWVETHLLERISQKGQVNLFETTIRVLGGLLSAYHLSG 240

Query: 241 GEYGK-TFENEGPKPAVYLEIAKNLADRLLSAFTSSPTAIPLCDVVLKDSSAHAAPGGSS 300
           GE G     + GPKP +YL IAK+LADRLLSAFTSSPT +P CDV+L +S+AH APGG+S
Sbjct: 241 GEQGTVNMTHVGPKPVIYLNIAKDLADRLLSAFTSSPTPVPFCDVILHESTAHPAPGGAS 300

Query: 301 STSEVSTLQLEFNYLSTVSGDPKYSIAAMKVLEHMKNLPKVEGLVPIYISPHSGQFSGET 360
           ST+EV+++QLEFNYLS++SGDPKYS  AMKVL H+K LPK EGLVPIYISP +G F GE 
Sbjct: 301 STAEVASVQLEFNYLSSISGDPKYSTEAMKVLAHIKTLPKTEGLVPIYISPQTGDFVGEN 360

Query: 361 IRLGSRGDSYYEYLLKVWLQKRTRENNNFTYLHDMYEEAMKGVKHLLVRKSVPKELVFVG 420
           IRLGSRGDSYYEYL+KVWLQ+  + N+NFTYLHDMY EAMKGV+HLLV+ S+PK LVFVG
Sbjct: 361 IRLGSRGDSYYEYLIKVWLQQGAKLNSNFTYLHDMYIEAMKGVRHLLVQNSIPKGLVFVG 420

Query: 421 ELPYGSGSAISPKMDHLVCFLSGTLALGATKGITKEEAMSKSLLSFDDLENLNLAEDLAK 480
           ELPYGS    SPKMDHLVCFL GTLALGATKG+TKE+A+ ++LLSF+DLENL LAEDLAK
Sbjct: 421 ELPYGSKGEFSPKMDHLVCFLPGTLALGATKGLTKEQALKENLLSFEDLENLKLAEDLAK 480

Query: 481 TCYEMYEVTSTGLAPEIVYFHTEEYSEQGFDGGNKNSKYIDDIIIKPLDRHNLMRPETVE 540
           TC+EMYEVT+TGLAPEI YFHT++Y+E G DGGNK+S Y +DIIIKP DRHNL+RPETVE
Sbjct: 481 TCFEMYEVTATGLAPEIAYFHTKDYTEDGLDGGNKSSMYANDIIIKPADRHNLLRPETVE 540

Query: 541 SLFVLYRITGNQKYRAWGWEIFEAFERHTKVSSGGYTSLDDVTTVPPRRRDKMETFFLGE 600
           SLFVLYRIT + KYR  GW+IFEAFE++TKV SGGYTSLDDVT VPP RRDKMETFFLGE
Sbjct: 541 SLFVLYRITKDTKYRDQGWQIFEAFEKYTKVKSGGYTSLDDVTEVPPHRRDKMETFFLGE 600

Query: 601 TLKYLYLLFGDSSTIPLDKYVFNTEAHPLPIEGN 631
           TLKYLYLLFGD S IPLDK+VFNTEAHPLPI  N
Sbjct: 601 TLKYLYLLFGDDSVIPLDKFVFNTEAHPLPIRRN 623

BLAST of Cla97C01G024530 vs. ExPASy Swiss-Prot
Match: Q9UKM7 (Endoplasmic reticulum mannosyl-oligosaccharide 1,2-alpha-mannosidase OS=Homo sapiens OX=9606 GN=MAN1B1 PE=1 SV=2)

HSP 1 Score: 405.2 bits (1040), Expect = 1.3e-111
Identity = 246/535 (45.98%), Postives = 329/535 (61.50%), Query Frame = 0

Query: 100 RRA--PRLPPRLPPDEKGSNTSYHIDPNMLISESLWISRQQKVKDAFIHAWSGYKRYAMG 159
           RRA  P  PP LPP  +   T  H++            RQ+ V D F+HAW GY+++A G
Sbjct: 224 RRAEVPTKPP-LPP-ARTQGTPVHLN-----------YRQKGVIDVFLHAWKGYRKFAWG 283

Query: 160 YDELMPLSQQGTDGLGGLGATVVDALDTAMIMGASDIVSEAGSWVEKHLLDRIKEKGQVN 219
           +DEL P+S+  ++   GLG T++DALDT  I+G      EA  WV K L    ++   VN
Sbjct: 284 HDELKPVSRSFSEWF-GLGLTLIDALDTMWILGLRKEFEEARKWVSKKL--HFEKDVDVN 343

Query: 220 LFETTIRVLGGLLSAYHLRAGEYGKTFENEGPKPAVYLEIAKNLADRLLSAFTSSPTAIP 279
           LFE+TIR+LGGLLSAYHL                +++L  A++  +RL+ AF  +P+ IP
Sbjct: 344 LFESTIRILGGLLSAYHLSG-------------DSLFLRKAEDFGNRLMPAF-RTPSKIP 403

Query: 280 LCDVVLKDSSAHAAPGGSSST-SEVSTLQLEFNYLSTVSGDPKYSIAAMKVLEHMKNLP- 339
             DV +    AH     S ST +EV+++QLEF  LS ++GD K+  A  KV +H+  L  
Sbjct: 404 YSDVNIGTGVAHPPRWTSDSTVAEVTSIQLEFRELSRLTGDKKFQEAVEKVTQHIHGLSG 463

Query: 340 KVEGLVPIYISPHSGQFSG-ETIRLGSRGDSYYEYLLKVWLQKRTRENNNFTYLHDMYEE 399
           K +GLVP++I+ HSG F+      LG+R DSYYEYLLK W+Q   +E    T L + Y E
Sbjct: 464 KKDGLVPMFINTHSGLFTHLGVFTLGARADSYYEYLLKQWIQGGKQE----TQLLEDYVE 523

Query: 400 AMKGVKHLLVRKSVPKELVFVGELPYGSGSAISPKMDHLVCFLSGTLALGATKGITKEEA 459
           A++GV+  L+R S P +L FVGEL +G  SA   KMDHLVCFL GTLALG   G+     
Sbjct: 524 AIEGVRTHLLRHSEPSKLTFVGELAHGRFSA---KMDHLVCFLPGTLALGVYHGL----- 583

Query: 460 MSKSLLSFDDLENLNLAEDLAKTCYEMYEVTSTGLAPEIVYFHTEEYSEQGFDGGNKNSK 519
                       ++ LA++L +TCY+M     TGL+PEIV+F+   Y + G         
Sbjct: 584 ---------PASHMELAQELMETCYQMNRQMETGLSPEIVHFNL--YPQPG--------- 643

Query: 520 YIDDIIIKPLDRHNLMRPETVESLFVLYRITGNQKYRAWGWEIFEAFERHTKVSSGGYTS 579
              D+ +KP DRHNL+RPETVESLF LYR+TG++KY+ WGWEI ++F R T+V SGGY+S
Sbjct: 644 -RRDVEVKPADRHNLLRPETVESLFYLYRVTGDRKYQDWGWEILQSFSRFTRVPSGGYSS 695

Query: 580 LDDV-TTVPPRRRDKMETFFLGETLKYLYLLFGDS-STIPLDKYVFNTEAHPLPI 628
           +++V     P  RDKME+FFLGETLKYL+LLF D  + + LD YVFNTEAHPLPI
Sbjct: 704 INNVQDPQKPEPRDKMESFFLGETLKYLFLLFSDDPNLLSLDAYVFNTEAHPLPI 695

BLAST of Cla97C01G024530 vs. ExPASy Swiss-Prot
Match: Q8J0Q0 (Mannosyl-oligosaccharide 1,2-alpha-mannosidase OS=Candida albicans OX=5476 GN=MNS1 PE=3 SV=2)

HSP 1 Score: 399.1 bits (1024), Expect = 9.7e-110
Identity = 238/509 (46.76%), Postives = 314/509 (61.69%), Query Frame = 0

Query: 130 ESLWISRQQKVKDAFIHAWSGYKRYAMGYDELMPLSQQGTD-GLGGLGATVVDALDTAMI 189
           +S W   Q +V+ A + +W  Y++Y  GYD   P+ Q+G + G   LG  +VD+LDT MI
Sbjct: 32  QSKWERAQSEVRQAILDSWHTYEKYGWGYDVYHPIKQEGENMGPKPLGWMIVDSLDTLMI 91

Query: 190 MGASDIVSEAGSWVEKHLLDRIKEKGQVNLFETTIRVLGGLLSAYHLRAGEYGKTFENEG 249
           M   + VS A  W+ K+ LD   +   VN FETTIR+LGGLLSAYH         F N+ 
Sbjct: 92  MDCPEEVSRARDWI-KNDLDYTFDY-NVNTFETTIRMLGGLLSAYH---------FSNDD 151

Query: 250 PKPAVYLEIAKNLADRLLSAFTSSPTAIPLCDVVLKDSSA--HAAPGGSSSTSEVSTLQL 309
               VYL+ A  LA+ L  A+  SP+ IP   V LK      +    G+SST+E +T+QL
Sbjct: 152 ----VYLDKAVQLANALHGAY-DSPSGIPYSSVNLKSGKGIKNHVDNGASSTAEAATVQL 211

Query: 310 EFNYLSTVSGDPKYSIAAMKVLEHMKNLPKVEGLVPIYISPHSGQFSGETIRLGSRGDSY 369
           E  YLS ++G+  +   A KV++ +++    +GLVPIY++P +G++ G  IRLGSRGDSY
Sbjct: 212 EMKYLSKLTGEILWWNLAEKVMQVLESNKPQDGLVPIYVNPDTGKYQGHLIRLGSRGDSY 271

Query: 370 YEYLLKVWLQKRTRENNNFTYLHDMYEEAMKGVKHLLVRKSVPKELVFVGELPYGSGSAI 429
           YEYLLK +LQ   +E        DMY E+++GVK  LV  S P  L F+GEL  G G  +
Sbjct: 272 YEYLLKQYLQTNKQE----LVYWDMYRESVEGVKKHLVSDSYPSGLTFIGELDNGIGGKL 331

Query: 430 SPKMDHLVCFLSGTLALGATKGITKEEAMSKSLLSFDDLENLNLAEDLAKTCYEMY-EVT 489
           S KMDHLVCF  G LALGAT G+T  EA S    + +   +  L E+L  TCY+MY +V+
Sbjct: 332 STKMDHLVCFYGGLLALGATGGLTLNEAQSLKSWNEEREADFKLGEELTYTCYKMYHDVS 391

Query: 490 STGLAPEIVYFHTEEYSEQGFDGGNKNSKYIDDIIIKPLDRHNLMRPETVESLFVLYRIT 549
            TGL+PEIV F             N+++    D IIKPLDRHNL RPETVESLF LYR+T
Sbjct: 392 PTGLSPEIVVF-------------NEDTSKSKDFIIKPLDRHNLQRPETVESLFYLYRLT 451

Query: 550 GNQKYRAWGWEIFEAFERHTKV--SSG--GYTSLDDVTTVP----PRRRDKMETFFLGET 609
           G+ KYR  G+EIF+ F ++TKV  S G   ++SL DVT+      P+ +D  E+F+  ET
Sbjct: 452 GDVKYREMGYEIFQNFIKYTKVVNSEGEVSFSSLSDVTSFDSNGLPKFKDNTESFWWAET 507

Query: 610 LKYLYLLFGDSSTIPLDKYVFNTEAHPLP 627
           LKYLYLLF D++ IPL  YVFNTEAHP P
Sbjct: 512 LKYLYLLFDDTNKIPLTDYVFNTEAHPFP 507

BLAST of Cla97C01G024530 vs. ExPASy Swiss-Prot
Match: B2GUY0 (Endoplasmic reticulum mannosyl-oligosaccharide 1,2-alpha-mannosidase OS=Rattus norvegicus OX=10116 GN=Man1b1 PE=2 SV=2)

HSP 1 Score: 391.7 bits (1005), Expect = 1.5e-107
Identity = 225/497 (45.27%), Postives = 319/497 (64.19%), Query Frame = 0

Query: 136 RQQKVKDAFIHAWSGYKRYAMGYDELMPLSQQGTDGLGGLGATVVDALDTAMIMGASDIV 195
           RQ+ V +AF+HAW GY+++A G+DEL P+S+  ++   GLG T++DALDT  I+G     
Sbjct: 207 RQKGVIEAFLHAWKGYQKFAWGHDELKPVSKTFSEWF-GLGLTLIDALDTMWILGLKQEF 266

Query: 196 SEAGSWVEKHLLDRIKEKGQVNLFETTIRVLGGLLSAYHLRAGEYGKTFENEGPKPAVYL 255
            EA  WV ++L    ++   VNLFE+TIR+LGGLLSAYHL                +++L
Sbjct: 267 KEARKWVSENL--DFQKNVDVNLFESTIRILGGLLSAYHLSG-------------DSLFL 326

Query: 256 EIAKNLADRLLSAFTSSPTAIPLCDVVLKDSSAHAAPGGSSST-SEVSTLQLEFNYLSTV 315
             A++  +RL+ AFT +P+ IP  DV +    AH+    S ST +EV+++QLEF  LS +
Sbjct: 327 SKAEDFGNRLMPAFT-TPSKIPYSDVNIGTGFAHSPQWTSDSTVAEVTSIQLEFRELSRL 386

Query: 316 SGDPKYSIAAMKVLEHMKNLP-KVEGLVPIYISPHSGQFSGETI-RLGSRGDSYYEYLLK 375
           +G  K+  A  +V +H+ +L  K +GLVP++I+ +SG F+   +  LG+R DSYYEYLLK
Sbjct: 387 TGIKKFQEAVEEVTKHIHSLSGKKDGLVPMFINTNSGLFTHPGVFTLGARADSYYEYLLK 446

Query: 376 VWLQKRTRENNNFTYLHDMYEEAMKGVKHLLVRKSVPKELVFVGELPYGSGSAISPKMDH 435
            W+Q   +E    T L + Y  A++G+K  L+R+S P++L FVGEL +G  SA   KMDH
Sbjct: 447 QWIQGGKKE----TQLLEDYVRAIEGIKAHLLRQSQPRKLTFVGELAHGRFSA---KMDH 506

Query: 436 LVCFLSGTLALGATKGITKEEAMSKSLLSFDDLENLNLAEDLAKTCYEMYEVTSTGLAPE 495
           LVCFL GTLALG   G+                ++++LA  L +TCY+M +   TGL+PE
Sbjct: 507 LVCFLPGTLALGVHHGL--------------PADHMDLARALMETCYQMNQQMETGLSPE 566

Query: 496 IVYFHTEEYSEQGFDGGNKNSKYIDDIIIKPLDRHNLMRPETVESLFVLYRITGNQKYRA 555
           I +F+    ++              D+ +KP DRHNL+RPETVESLF LYR+T ++KY+ 
Sbjct: 567 IAHFNMYPRADH------------KDVEVKPADRHNLLRPETVESLFYLYRVTKDRKYQD 626

Query: 556 WGWEIFEAFERHTKVSSGGYTSLDDV-TTVPPRRRDKMETFFLGETLKYLYLLFGDS-ST 615
           WGWEI ++F ++T+V SGGY+S+++V  +  P  RDKME+FF+GETLKYLYLLF D    
Sbjct: 627 WGWEILQSFNKYTRVPSGGYSSINNVQNSHKPEPRDKMESFFVGETLKYLYLLFSDDLEL 653

Query: 616 IPLDKYVFNTEAHPLPI 628
           + LD  VFNTEAHPLPI
Sbjct: 687 LGLDTCVFNTEAHPLPI 653

BLAST of Cla97C01G024530 vs. ExPASy Swiss-Prot
Match: A2AJ15 (Endoplasmic reticulum mannosyl-oligosaccharide 1,2-alpha-mannosidase OS=Mus musculus OX=10090 GN=Man1b1 PE=1 SV=1)

HSP 1 Score: 389.0 bits (998), Expect = 1.0e-106
Identity = 223/497 (44.87%), Postives = 318/497 (63.98%), Query Frame = 0

Query: 136 RQQKVKDAFIHAWSGYKRYAMGYDELMPLSQQGTDGLGGLGATVVDALDTAMIMGASDIV 195
           RQ+ V +AF+HAW GY+++A G+DEL P+S+  ++   GLG T++DALDT  I+G     
Sbjct: 208 RQKGVIEAFLHAWKGYQKFAWGHDELKPVSKTFSEWF-GLGLTLIDALDTMWILGLKQEF 267

Query: 196 SEAGSWVEKHLLDRIKEKGQVNLFETTIRVLGGLLSAYHLRAGEYGKTFENEGPKPAVYL 255
            +A  WV ++L    ++   VNLFE+TIR+LGGLLS YHL                +++L
Sbjct: 268 KQARKWVSENL--DFQKNVDVNLFESTIRILGGLLSTYHLSG-------------DSLFL 327

Query: 256 EIAKNLADRLLSAFTSSPTAIPLCDVVLKDSSAHAAPGGSSST-SEVSTLQLEFNYLSTV 315
             A++   RL+ AFT +P+ IP  DV +    AH+    S ST +EV+++QLEF  LS +
Sbjct: 328 TKAEDFGKRLMPAFT-TPSKIPYSDVNIGTGFAHSPQWTSDSTVAEVTSIQLEFRELSRL 387

Query: 316 SGDPKYSIAAMKVLEHMKNLP-KVEGLVPIYISPHSGQFSGETI-RLGSRGDSYYEYLLK 375
           +G  K+  A  +V +H+ +L  K +GLVP++I+ +SG F+   +  LG+R DSYYEYLLK
Sbjct: 388 TGIKKFQEAVEEVTKHIHSLSGKKDGLVPMFINTNSGLFTHPGVFTLGARADSYYEYLLK 447

Query: 376 VWLQKRTRENNNFTYLHDMYEEAMKGVKHLLVRKSVPKELVFVGELPYGSGSAISPKMDH 435
            W+Q   +E    T L + Y +A++G+K  L+R+S P++L FVGEL +G  SA   KMDH
Sbjct: 448 QWIQGGKKE----TQLLEDYVKAIEGIKAHLLRQSQPRKLTFVGELAHGRFSA---KMDH 507

Query: 436 LVCFLSGTLALGATKGITKEEAMSKSLLSFDDLENLNLAEDLAKTCYEMYEVTSTGLAPE 495
           LVCFL GTLALG   G+                ++++LA  L +TCY+M +   TGL+PE
Sbjct: 508 LVCFLPGTLALGVHHGL--------------PADHMDLARALMETCYQMNQQMETGLSPE 567

Query: 496 IVYFHTEEYSEQGFDGGNKNSKYIDDIIIKPLDRHNLMRPETVESLFVLYRITGNQKYRA 555
           I +F+    ++              D+ +KP DRHNL+RPETVESLF LYR+T ++KY+ 
Sbjct: 568 IAHFNMYPRADH------------KDVEVKPADRHNLLRPETVESLFYLYRVTRDRKYQD 627

Query: 556 WGWEIFEAFERHTKVSSGGYTSLDDV-TTVPPRRRDKMETFFLGETLKYLYLLFGDS-ST 615
           WGWEI ++F ++T+V SGGY+S+++V  +  P  RDKME+FF+GETLKYLYLLF D    
Sbjct: 628 WGWEILQSFNKYTRVPSGGYSSINNVQNSHKPEPRDKMESFFVGETLKYLYLLFSDDLEL 654

Query: 616 IPLDKYVFNTEAHPLPI 628
           + LD  VFNTEAHPLPI
Sbjct: 688 LSLDSCVFNTEAHPLPI 654

BLAST of Cla97C01G024530 vs. ExPASy TrEMBL
Match: A0A1S3AZ69 (alpha-1,2-Mannosidase OS=Cucumis melo OX=3656 GN=LOC103484372 PE=3 SV=1)

HSP 1 Score: 1219.5 bits (3154), Expect = 0.0e+00
Identity = 600/634 (94.64%), Postives = 617/634 (97.32%), Query Frame = 0

Query: 1   MSSSLPYSVKDVHYDNAKFRQRSYFKVMTQNLLTGSRKRDYLSCSTGKFLVLLLILGLGY 60
           MSSSLPYSVKDVHYDNAKFRQRSY KVMTQNLLTGSRKRDYLSCSTGKFL+LL+I GL Y
Sbjct: 1   MSSSLPYSVKDVHYDNAKFRQRSYLKVMTQNLLTGSRKRDYLSCSTGKFLILLMIFGLAY 60

Query: 61  LLLTSTTPVHVPNRLAKVSENNEKGELKNNGGINFRNLWRRAPRLPPRLPPDEKGSNTSY 120
           LLLT+ TPVHVPN+ AKVSENNEK E+KNNGGINFRNLWR+APRLPP+LPPDEKGSNTSY
Sbjct: 61  LLLTNATPVHVPNQPAKVSENNEKREIKNNGGINFRNLWRKAPRLPPQLPPDEKGSNTSY 120

Query: 121 HIDPNMLISESLWISRQQKVKDAFIHAWSGYKRYAMGYDELMPLSQQGTDGLGGLGATVV 180
           HIDPN LISESLWISRQQKVKDAFIHAWSGYKRYAMGYDELMPLSQ GTDGLGGLGATVV
Sbjct: 121 HIDPNTLISESLWISRQQKVKDAFIHAWSGYKRYAMGYDELMPLSQHGTDGLGGLGATVV 180

Query: 181 DALDTAMIMGASDIVSEAGSWVEKHLLDRIKEKGQVNLFETTIRVLGGLLSAYHLRAGEY 240
           DALDTAMIMGASDIVSEAGSW+EKHL+DRIKEKGQVNLFETTIRV+GGLLSAYHLRAGEY
Sbjct: 181 DALDTAMIMGASDIVSEAGSWIEKHLMDRIKEKGQVNLFETTIRVVGGLLSAYHLRAGEY 240

Query: 241 GKTFENEGPKPAVYLEIAKNLADRLLSAFTSSPTAIPLCDVVLKDSSAHAAPGGSSSTSE 300
           GKTF NEGPKPAVYLEIAKNLADRLLSAFTSSPTAIPLCDVVLKDSSAHAAPGGSSSTSE
Sbjct: 241 GKTFGNEGPKPAVYLEIAKNLADRLLSAFTSSPTAIPLCDVVLKDSSAHAAPGGSSSTSE 300

Query: 301 VSTLQLEFNYLSTVSGDPKYSIAAMKVLEHMKNLPKVEGLVPIYISPHSGQFSGETIRLG 360
           VSTLQLEFNYLSTVSGDPKYSIAAMKVLEHMK+LPKVEGLVPIYISPH+G+FSGETIRLG
Sbjct: 301 VSTLQLEFNYLSTVSGDPKYSIAAMKVLEHMKSLPKVEGLVPIYISPHTGEFSGETIRLG 360

Query: 361 SRGDSYYEYLLKVWLQKRTRENNNFTYLHDMYEEAMKGVKHLLVRKSVPKELVFVGELPY 420
           SRGDSYYEYLLKVWLQKRT  NNNFTYLHDMYEEAMKGVKHLLVRKS+P  LVFVGELPY
Sbjct: 361 SRGDSYYEYLLKVWLQKRTSGNNNFTYLHDMYEEAMKGVKHLLVRKSIPNGLVFVGELPY 420

Query: 421 GSGSAISPKMDHLVCFLSGTLALGATKGITKEEAMSKSLLSFDDLENLNLAEDLAKTCYE 480
           GS SAISPKMDHLVCFLSGTLALGATKGITKEEAMSKSLLSFDDLENLNLAEDLAKTC+E
Sbjct: 421 GSDSAISPKMDHLVCFLSGTLALGATKGITKEEAMSKSLLSFDDLENLNLAEDLAKTCFE 480

Query: 481 MYEVTSTGLAPEIVYFHTEEYSEQGFDGGNKNSKYIDDIIIKPLDRHNLMRPETVESLFV 540
           MYEVTSTGLAPEI YFHTEEYSEQG DGGNKNSKYIDDIIIKPLDRHNLMRPETVESLFV
Sbjct: 481 MYEVTSTGLAPEIAYFHTEEYSEQGHDGGNKNSKYIDDIIIKPLDRHNLMRPETVESLFV 540

Query: 541 LYRITGNQKYRAWGWEIFEAFERHTKVSSGGYTSLDDVTTVPPRRRDKMETFFLGETLKY 600
           LYRITGN KYRAWGWEIFEAFERHTKVSSGGYTSLDDVTTVPPRRRDKMETFFLGETLKY
Sbjct: 541 LYRITGNPKYRAWGWEIFEAFERHTKVSSGGYTSLDDVTTVPPRRRDKMETFFLGETLKY 600

Query: 601 LYLLFGDSSTIPLDKYVFNTEAHPLPIEGNIQIK 635
           LYLLFGDSSTIPLDKYVFNTEAHPLP+EGNIQI+
Sbjct: 601 LYLLFGDSSTIPLDKYVFNTEAHPLPMEGNIQIQ 634

BLAST of Cla97C01G024530 vs. ExPASy TrEMBL
Match: A0A0A0KLY9 (alpha-1,2-Mannosidase OS=Cucumis sativus OX=3659 GN=Csa_6G523340 PE=3 SV=1)

HSP 1 Score: 1209.1 bits (3127), Expect = 0.0e+00
Identity = 594/634 (93.69%), Postives = 616/634 (97.16%), Query Frame = 0

Query: 1   MSSSLPYSVKDVHYDNAKFRQRSYFKVMTQNLLTGSRKRDYLSCSTGKFLVLLLILGLGY 60
           MSSSLPYSVKDVHYDNAKFRQRSY KVMTQNLLTGSRKRDYLSCSTGKFLVLLLI GL Y
Sbjct: 1   MSSSLPYSVKDVHYDNAKFRQRSYVKVMTQNLLTGSRKRDYLSCSTGKFLVLLLIFGLAY 60

Query: 61  LLLTSTTPVHVPNRLAKVSENNEKGELKNNGGINFRNLWRRAPRLPPRLPPDEKGSNTSY 120
           LLLTS TPVHVPN+LAKVSEN+EK E+KNNGGINFRNLWR+APRLPP+LPPDEKGSNTSY
Sbjct: 61  LLLTSATPVHVPNKLAKVSENSEKQEIKNNGGINFRNLWRKAPRLPPQLPPDEKGSNTSY 120

Query: 121 HIDPNMLISESLWISRQQKVKDAFIHAWSGYKRYAMGYDELMPLSQQGTDGLGGLGATVV 180
           HIDPN LISESLWISRQQKVKDAFIHAWSGYKRYAMGYDELMPLSQ GTDGLGGLGATVV
Sbjct: 121 HIDPNTLISESLWISRQQKVKDAFIHAWSGYKRYAMGYDELMPLSQHGTDGLGGLGATVV 180

Query: 181 DALDTAMIMGASDIVSEAGSWVEKHLLDRIKEKGQVNLFETTIRVLGGLLSAYHLRAGEY 240
           DALDTAMIMGASDIVSEAGSW+EK+L+DRIKEKGQVNLFETTIR++GGLLSAYHLRAGEY
Sbjct: 181 DALDTAMIMGASDIVSEAGSWIEKNLMDRIKEKGQVNLFETTIRIVGGLLSAYHLRAGEY 240

Query: 241 GKTFENEGPKPAVYLEIAKNLADRLLSAFTSSPTAIPLCDVVLKDSSAHAAPGGSSSTSE 300
           GKTF NEGPKPAVYLEIAKNLADRLLSAFTSSPTAIPLCDVVLKDSSAHAAPGGSSSTSE
Sbjct: 241 GKTFGNEGPKPAVYLEIAKNLADRLLSAFTSSPTAIPLCDVVLKDSSAHAAPGGSSSTSE 300

Query: 301 VSTLQLEFNYLSTVSGDPKYSIAAMKVLEHMKNLPKVEGLVPIYISPHSGQFSGETIRLG 360
           VSTLQLEFNYLSTVSGDPKYSIAAMKVLEHMK+LPKVEGLVPIYISPH+G+FSGETIRLG
Sbjct: 301 VSTLQLEFNYLSTVSGDPKYSIAAMKVLEHMKSLPKVEGLVPIYISPHTGKFSGETIRLG 360

Query: 361 SRGDSYYEYLLKVWLQKRTRENNNFTYLHDMYEEAMKGVKHLLVRKSVPKELVFVGELPY 420
           SRGDSYYEYLLKVWLQKRT  N+NFTYLHDMYEEAMKGVKHLLVRKS+P  LVFVGELPY
Sbjct: 361 SRGDSYYEYLLKVWLQKRTSGNSNFTYLHDMYEEAMKGVKHLLVRKSIPNGLVFVGELPY 420

Query: 421 GSGSAISPKMDHLVCFLSGTLALGATKGITKEEAMSKSLLSFDDLENLNLAEDLAKTCYE 480
           GS S ISPKMDHLVCFLSGTLALGATKGITKEEAMSKSLL+FDDLENLNLAEDLAKTC+E
Sbjct: 421 GSDSTISPKMDHLVCFLSGTLALGATKGITKEEAMSKSLLNFDDLENLNLAEDLAKTCFE 480

Query: 481 MYEVTSTGLAPEIVYFHTEEYSEQGFDGGNKNSKYIDDIIIKPLDRHNLMRPETVESLFV 540
           MYEVTSTGLAPEI YFHTEEYSEQG DGGNK SKYIDDIIIKPLDRHNLMRPETVESLFV
Sbjct: 481 MYEVTSTGLAPEIAYFHTEEYSEQGLDGGNKKSKYIDDIIIKPLDRHNLMRPETVESLFV 540

Query: 541 LYRITGNQKYRAWGWEIFEAFERHTKVSSGGYTSLDDVTTVPPRRRDKMETFFLGETLKY 600
           LYRITG+ KYRAWGWEIFEAFERHTKVSSGGYTSLDDVTT+PPRRRDKMETFFLGETLKY
Sbjct: 541 LYRITGDPKYRAWGWEIFEAFERHTKVSSGGYTSLDDVTTIPPRRRDKMETFFLGETLKY 600

Query: 601 LYLLFGDSSTIPLDKYVFNTEAHPLPIEGNIQIK 635
           LYLLFGDSSTIPLDKYVFNTEAHPLP++GNIQI+
Sbjct: 601 LYLLFGDSSTIPLDKYVFNTEAHPLPMDGNIQIQ 634

BLAST of Cla97C01G024530 vs. ExPASy TrEMBL
Match: A0A6J1KW66 (alpha-1,2-Mannosidase OS=Cucurbita maxima OX=3661 GN=LOC111496964 PE=3 SV=1)

HSP 1 Score: 1179.9 bits (3051), Expect = 0.0e+00
Identity = 584/633 (92.26%), Postives = 604/633 (95.42%), Query Frame = 0

Query: 1   MSSSLPYSVKDVHYDNAKFRQRSYFKVMTQNLLTGSRKRDYLSCSTGKFLVLLLILGLGY 60
           M SSLPYSVKDVHYDNAKFRQRSYFKVMTQNL TGSRKRDYLSCSTGKFLVLLLI GL Y
Sbjct: 1   MRSSLPYSVKDVHYDNAKFRQRSYFKVMTQNLFTGSRKRDYLSCSTGKFLVLLLIFGLAY 60

Query: 61  LLLTSTTP-VHVPNRLAKVSENNEKGELKNNGGINFRNLWRRAPRLPPRLPPDEKGSNTS 120
           LLLTSTTP   VPN+L KV ENN+KGE KNNGGINFRNLWR+ PRLPPRLPPDEKGSNTS
Sbjct: 61  LLLTSTTPGDFVPNQLDKVRENNQKGEFKNNGGINFRNLWRKPPRLPPRLPPDEKGSNTS 120

Query: 121 YHIDPNMLISESLWISRQQKVKDAFIHAWSGYKRYAMGYDELMPLSQQGTDGLGGLGATV 180
           YHIDPN LISESLWISRQQKVKDAFIHAWSGYKRYAMG+DELMPLSQQGTDGLGGLGATV
Sbjct: 121 YHIDPNTLISESLWISRQQKVKDAFIHAWSGYKRYAMGHDELMPLSQQGTDGLGGLGATV 180

Query: 181 VDALDTAMIMGASDIVSEAGSWVEKHLLDRIKEKGQVNLFETTIRVLGGLLSAYHLRAGE 240
           VDALDTAMIMGASDIVSEAGSWVEKHLLDRIKEKGQVNLFETTIRVLGGLLSAYHLRAGE
Sbjct: 181 VDALDTAMIMGASDIVSEAGSWVEKHLLDRIKEKGQVNLFETTIRVLGGLLSAYHLRAGE 240

Query: 241 YGKTFENEGPKPAVYLEIAKNLADRLLSAFTSSPTAIPLCDVVLKDSSAHAAPGGSSSTS 300
           YGKTFENEGPKPAVYLE AKNLADRLLSAFTSSPTAIPLCDVVL+DSSAHAAPGG SSTS
Sbjct: 241 YGKTFENEGPKPAVYLETAKNLADRLLSAFTSSPTAIPLCDVVLRDSSAHAAPGGLSSTS 300

Query: 301 EVSTLQLEFNYLSTVSGDPKYSIAAMKVLEHMKNLPKVEGLVPIYISPHSGQFSGETIRL 360
           EVSTLQLEFNYLST+SGDPKYSIAAMKVLEHMK+LPK+EGLVPIYISP SGQFSGETIRL
Sbjct: 301 EVSTLQLEFNYLSTISGDPKYSIAAMKVLEHMKSLPKLEGLVPIYISPDSGQFSGETIRL 360

Query: 361 GSRGDSYYEYLLKVWLQKRTRENNNFTYLHDMYEEAMKGVKHLLVRKSVPKELVFVGELP 420
           GSRGDSYYEYLLKVWLQK  RENNN TYLHDMYEEAMKGVKHLLVRKSVP  LVFVGELP
Sbjct: 361 GSRGDSYYEYLLKVWLQKGARENNNLTYLHDMYEEAMKGVKHLLVRKSVPNGLVFVGELP 420

Query: 421 YGSGSAISPKMDHLVCFLSGTLALGATKGITKEEAMSKSLLSFDDLENLNLAEDLAKTCY 480
           YGS  AISPKMDHLVCFL GTLALGATKGI+KEE+M KSLLSF+DLENLNLAEDLAKTC+
Sbjct: 421 YGSSEAISPKMDHLVCFLPGTLALGATKGISKEESMKKSLLSFEDLENLNLAEDLAKTCF 480

Query: 481 EMYEVTSTGLAPEIVYFHTEEYSEQGFDGGNKNSKYIDDIIIKPLDRHNLMRPETVESLF 540
           EMYEVT+TGLAPEIVYFHTEEYSE G DGGNKN+KYIDDIIIKP DRHNL+RPETVESLF
Sbjct: 481 EMYEVTATGLAPEIVYFHTEEYSEGGHDGGNKNAKYIDDIIIKPPDRHNLLRPETVESLF 540

Query: 541 VLYRITGNQKYRAWGWEIFEAFERHTKVSSGGYTSLDDVTTVPPRRRDKMETFFLGETLK 600
           VLYRITG+ KYRAWGW+IFEAFERHTKV++GGYTSLDDVTTVPPRRRDKMETFFLGETLK
Sbjct: 541 VLYRITGDPKYRAWGWQIFEAFERHTKVNTGGYTSLDDVTTVPPRRRDKMETFFLGETLK 600

Query: 601 YLYLLFGDSSTIPLDKYVFNTEAHPLPIEGNIQ 633
           YLYLLFGD+STIPLDKYVFNTEAHPLPIE NIQ
Sbjct: 601 YLYLLFGDNSTIPLDKYVFNTEAHPLPIETNIQ 633

BLAST of Cla97C01G024530 vs. ExPASy TrEMBL
Match: A0A6J1ELX6 (alpha-1,2-Mannosidase OS=Cucurbita moschata OX=3662 GN=LOC111433756 PE=3 SV=1)

HSP 1 Score: 1178.3 bits (3047), Expect = 0.0e+00
Identity = 584/633 (92.26%), Postives = 604/633 (95.42%), Query Frame = 0

Query: 1   MSSSLPYSVKDVHYDNAKFRQRSYFKVMTQNLLTGSRKRDYLSCSTGKFLVLLLILGLGY 60
           M SSLPYSVKDVHYDNAKFRQRSYFKVMTQNLLTGSRKRDYLSCSTGKFLVLLLI GL Y
Sbjct: 1   MRSSLPYSVKDVHYDNAKFRQRSYFKVMTQNLLTGSRKRDYLSCSTGKFLVLLLIFGLAY 60

Query: 61  LLLTSTTP-VHVPNRLAKVSENNEKGELKNNGGINFRNLWRRAPRLPPRLPPDEKGSNTS 120
           LLLTSTTP   VPNRL KVSENN+KGE K+NGGINFRNLWR+ PRLPPRLPPDEKGSNTS
Sbjct: 61  LLLTSTTPGDFVPNRLDKVSENNQKGEFKSNGGINFRNLWRKPPRLPPRLPPDEKGSNTS 120

Query: 121 YHIDPNMLISESLWISRQQKVKDAFIHAWSGYKRYAMGYDELMPLSQQGTDGLGGLGATV 180
           YHIDPN LISESLWISRQQKVKDAFIHAWSGYKRYAMG+DELMPLSQQGTDGLGGLGATV
Sbjct: 121 YHIDPNTLISESLWISRQQKVKDAFIHAWSGYKRYAMGHDELMPLSQQGTDGLGGLGATV 180

Query: 181 VDALDTAMIMGASDIVSEAGSWVEKHLLDRIKEKGQVNLFETTIRVLGGLLSAYHLRAGE 240
           VDALDTAMIMGASDIVSEAGSWVEKHLLDRIKEKGQVNLFETTIRVLGGLLSAYHLRAGE
Sbjct: 181 VDALDTAMIMGASDIVSEAGSWVEKHLLDRIKEKGQVNLFETTIRVLGGLLSAYHLRAGE 240

Query: 241 YGKTFENEGPKPAVYLEIAKNLADRLLSAFTSSPTAIPLCDVVLKDSSAHAAPGGSSSTS 300
           YGKTFENEGP PA YLE AKNLADRLLSAFTSSPTAIPLCDVVL+DSSAHAAPGG SSTS
Sbjct: 241 YGKTFENEGPTPAAYLETAKNLADRLLSAFTSSPTAIPLCDVVLRDSSAHAAPGGLSSTS 300

Query: 301 EVSTLQLEFNYLSTVSGDPKYSIAAMKVLEHMKNLPKVEGLVPIYISPHSGQFSGETIRL 360
           EVSTLQLEFNYLST+SGDPKYSIAAMKVLEHMK+LPK+EGLVPIYISP SGQFSGETIRL
Sbjct: 301 EVSTLQLEFNYLSTISGDPKYSIAAMKVLEHMKSLPKLEGLVPIYISPDSGQFSGETIRL 360

Query: 361 GSRGDSYYEYLLKVWLQKRTRENNNFTYLHDMYEEAMKGVKHLLVRKSVPKELVFVGELP 420
           GSRGDSYYEYLLKVWLQK  REN+N TYLHDMYEEAMKGVKHLLVRKSVP  LVFVGELP
Sbjct: 361 GSRGDSYYEYLLKVWLQKGARENHNLTYLHDMYEEAMKGVKHLLVRKSVPNGLVFVGELP 420

Query: 421 YGSGSAISPKMDHLVCFLSGTLALGATKGITKEEAMSKSLLSFDDLENLNLAEDLAKTCY 480
           YGS  AISPKMDHLVCFL GTLALGATKGI+KEEAM KSLLSF+DLENLNLAEDLAKTC+
Sbjct: 421 YGSSEAISPKMDHLVCFLPGTLALGATKGISKEEAMKKSLLSFEDLENLNLAEDLAKTCF 480

Query: 481 EMYEVTSTGLAPEIVYFHTEEYSEQGFDGGNKNSKYIDDIIIKPLDRHNLMRPETVESLF 540
           EMYEVT+TGLAPEIVYFHTEEYSE G DGGNKN+KYIDDIIIKP DRHNL+RPETVESLF
Sbjct: 481 EMYEVTATGLAPEIVYFHTEEYSEGGHDGGNKNAKYIDDIIIKPPDRHNLLRPETVESLF 540

Query: 541 VLYRITGNQKYRAWGWEIFEAFERHTKVSSGGYTSLDDVTTVPPRRRDKMETFFLGETLK 600
           VLYRITG+ KYRAWGW+IFEAFERHTKV++GGYTSLDDVTTVPPRRRDKMETFFLGETLK
Sbjct: 541 VLYRITGDPKYRAWGWQIFEAFERHTKVNTGGYTSLDDVTTVPPRRRDKMETFFLGETLK 600

Query: 601 YLYLLFGDSSTIPLDKYVFNTEAHPLPIEGNIQ 633
           YLYLLFGD+STIPLDKYVFNTEAHPLPIE NIQ
Sbjct: 601 YLYLLFGDNSTIPLDKYVFNTEAHPLPIETNIQ 633

BLAST of Cla97C01G024530 vs. ExPASy TrEMBL
Match: A0A5A7UEF9 (alpha-1,2-Mannosidase OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold255G008980 PE=3 SV=1)

HSP 1 Score: 1168.3 bits (3021), Expect = 0.0e+00
Identity = 574/607 (94.56%), Postives = 591/607 (97.36%), Query Frame = 0

Query: 28  MTQNLLTGSRKRDYLSCSTGKFLVLLLILGLGYLLLTSTTPVHVPNRLAKVSENNEKGEL 87
           MTQNLLTGSRKRDYLSCSTGKFL+LL+I GL YLLLT+ TPVHVPN+ AKVSENNEK E+
Sbjct: 1   MTQNLLTGSRKRDYLSCSTGKFLILLMIFGLAYLLLTNATPVHVPNQPAKVSENNEKREI 60

Query: 88  KNNGGINFRNLWRRAPRLPPRLPPDEKGSNTSYHIDPNMLISESLWISRQQKVKDAFIHA 147
           KNNGGINFRNLWR+APRLPP+LPPDEKGSNTSYHIDPN LISESLWISRQQKVKDAFIHA
Sbjct: 61  KNNGGINFRNLWRKAPRLPPQLPPDEKGSNTSYHIDPNTLISESLWISRQQKVKDAFIHA 120

Query: 148 WSGYKRYAMGYDELMPLSQQGTDGLGGLGATVVDALDTAMIMGASDIVSEAGSWVEKHLL 207
           WSGYKRYAMGYDELMPLSQ GTDGLGGLGATVVDALDTAMIMGASDIVSEAGSW+EKHL+
Sbjct: 121 WSGYKRYAMGYDELMPLSQHGTDGLGGLGATVVDALDTAMIMGASDIVSEAGSWIEKHLM 180

Query: 208 DRIKEKGQVNLFETTIRVLGGLLSAYHLRAGEYGKTFENEGPKPAVYLEIAKNLADRLLS 267
           DRIKEKGQVNLFETTIRV+GGLLSAYHLRAGEYGKTF NEGPKPAVYLEIAKNLADRLLS
Sbjct: 181 DRIKEKGQVNLFETTIRVVGGLLSAYHLRAGEYGKTFGNEGPKPAVYLEIAKNLADRLLS 240

Query: 268 AFTSSPTAIPLCDVVLKDSSAHAAPGGSSSTSEVSTLQLEFNYLSTVSGDPKYSIAAMKV 327
           AFTSSPTAIPLCDVVLKDSSAHAAPGGSSSTSEVSTLQLEFNYLSTVSGDPKYSIAAMKV
Sbjct: 241 AFTSSPTAIPLCDVVLKDSSAHAAPGGSSSTSEVSTLQLEFNYLSTVSGDPKYSIAAMKV 300

Query: 328 LEHMKNLPKVEGLVPIYISPHSGQFSGETIRLGSRGDSYYEYLLKVWLQKRTRENNNFTY 387
           LEHMK+LPKVEGLVPIYISPH+G+FSGETIRLGSRGDSYYEYLLKVWLQKRT  NNNFTY
Sbjct: 301 LEHMKSLPKVEGLVPIYISPHTGEFSGETIRLGSRGDSYYEYLLKVWLQKRTSGNNNFTY 360

Query: 388 LHDMYEEAMKGVKHLLVRKSVPKELVFVGELPYGSGSAISPKMDHLVCFLSGTLALGATK 447
           LHDMYEEAMKGVKHLLVRKS+P  LVFVGELPYGS SAISPKMDHLVCFLSGTLALGATK
Sbjct: 361 LHDMYEEAMKGVKHLLVRKSIPNGLVFVGELPYGSDSAISPKMDHLVCFLSGTLALGATK 420

Query: 448 GITKEEAMSKSLLSFDDLENLNLAEDLAKTCYEMYEVTSTGLAPEIVYFHTEEYSEQGFD 507
           GITKEEAMSKSLLSFDDLENLNLAEDLAKTC+EMYEVTSTGLAPEI YFHTEEYSEQG D
Sbjct: 421 GITKEEAMSKSLLSFDDLENLNLAEDLAKTCFEMYEVTSTGLAPEIAYFHTEEYSEQGHD 480

Query: 508 GGNKNSKYIDDIIIKPLDRHNLMRPETVESLFVLYRITGNQKYRAWGWEIFEAFERHTKV 567
           GGNKNSKYIDDIIIKPLDRHNLMRPETVESLFVLYRITGN KYRAWGWEIFEAFERHTKV
Sbjct: 481 GGNKNSKYIDDIIIKPLDRHNLMRPETVESLFVLYRITGNPKYRAWGWEIFEAFERHTKV 540

Query: 568 SSGGYTSLDDVTTVPPRRRDKMETFFLGETLKYLYLLFGDSSTIPLDKYVFNTEAHPLPI 627
           SSGGYTSLDDVTTVPPRRRDKMETFFLGETLKYLYLLFGDSSTIPLDKYVFNTEAHPLP+
Sbjct: 541 SSGGYTSLDDVTTVPPRRRDKMETFFLGETLKYLYLLFGDSSTIPLDKYVFNTEAHPLPM 600

Query: 628 EGNIQIK 635
           EGNIQI+
Sbjct: 601 EGNIQIQ 607

BLAST of Cla97C01G024530 vs. TAIR 10
Match: AT1G30000.1 (alpha-mannosidase 3 )

HSP 1 Score: 914.1 bits (2361), Expect = 6.3e-266
Identity = 455/634 (71.77%), Postives = 523/634 (82.49%), Query Frame = 0

Query: 1   MSSSLPYSVKDVHYDNAKFRQRSYFKVMTQNLLTGSRKRDYLSCSTGKFLVLLLILGLGY 60
           MS SLPYSVKD+HYDNAKFR RS  KV +Q+LLT S KR+Y SCSTGKFL+L+L  G+  
Sbjct: 1   MSKSLPYSVKDIHYDNAKFRHRSPLKVFSQSLLTLSTKRNYASCSTGKFLILILFFGVAC 60

Query: 61  LLLTSTTPVHVPNRLAKVSENNEKGELKNNGGINFRNLWRRAPRLPPRLPPDE---KGSN 120
           L+L S +P          S  NEKG++   GG+    L R+ PRLPPRL PDE   +GS+
Sbjct: 61  LMLMSKSP--------NESGLNEKGKVTFVGGLRLGGLLRKPPRLPPRLSPDEGQLRGSS 120

Query: 121 TSYHIDPNMLISESLWISRQQKVKDAFIHAWSGYKRYAMGYDELMPLSQQGTDGLGGLGA 180
           T+     N   S+  W +RQQ VK+AF HAWSGY++YAMGYDELMP+SQ+G DGLGGLGA
Sbjct: 121 TNGSTISN---SDPKWAARQQSVKEAFDHAWSGYRKYAMGYDELMPISQKGVDGLGGLGA 180

Query: 181 TVVDALDTAMIMGASDIVSEAGSWVEKHLLDRIKEKGQVNLFETTIRVLGGLLSAYHLRA 240
           TVVDALDTAMIMG  +IVSEAGSWVE HLL+RI +KGQVNLFETTIRVLGGLLSAYHL  
Sbjct: 181 TVVDALDTAMIMGLDNIVSEAGSWVETHLLERISQKGQVNLFETTIRVLGGLLSAYHLSG 240

Query: 241 GEYGK-TFENEGPKPAVYLEIAKNLADRLLSAFTSSPTAIPLCDVVLKDSSAHAAPGGSS 300
           GE G     + GPKP +YL IAK+LADRLLSAFTSSPT +P CDV+L +S+AH APGG+S
Sbjct: 241 GEQGTVNMTHVGPKPVIYLNIAKDLADRLLSAFTSSPTPVPFCDVILHESTAHPAPGGAS 300

Query: 301 STSEVSTLQLEFNYLSTVSGDPKYSIAAMKVLEHMKNLPKVEGLVPIYISPHSGQFSGET 360
           ST+EV+++QLEFNYLS++SGDPKYS  AMKVL H+K LPK EGLVPIYISP +G F GE 
Sbjct: 301 STAEVASVQLEFNYLSSISGDPKYSTEAMKVLAHIKTLPKTEGLVPIYISPQTGDFVGEN 360

Query: 361 IRLGSRGDSYYEYLLKVWLQKRTRENNNFTYLHDMYEEAMKGVKHLLVRKSVPKELVFVG 420
           IRLGSRGDSYYEYL+KVWLQ+  + N+NFTYLHDMY EAMKGV+HLLV+ S+PK LVFVG
Sbjct: 361 IRLGSRGDSYYEYLIKVWLQQGAKLNSNFTYLHDMYIEAMKGVRHLLVQNSIPKGLVFVG 420

Query: 421 ELPYGSGSAISPKMDHLVCFLSGTLALGATKGITKEEAMSKSLLSFDDLENLNLAEDLAK 480
           ELPYGS    SPKMDHLVCFL GTLALGATKG+TKE+A+ ++LLSF+DLENL LAEDLAK
Sbjct: 421 ELPYGSKGEFSPKMDHLVCFLPGTLALGATKGLTKEQALKENLLSFEDLENLKLAEDLAK 480

Query: 481 TCYEMYEVTSTGLAPEIVYFHTEEYSEQGFDGGNKNSKYIDDIIIKPLDRHNLMRPETVE 540
           TC+EMYEVT+TGLAPEI YFHT++Y+E G DGGNK+S Y +DIIIKP DRHNL+RPETVE
Sbjct: 481 TCFEMYEVTATGLAPEIAYFHTKDYTEDGLDGGNKSSMYANDIIIKPADRHNLLRPETVE 540

Query: 541 SLFVLYRITGNQKYRAWGWEIFEAFERHTKVSSGGYTSLDDVTTVPPRRRDKMETFFLGE 600
           SLFVLYRIT + KYR  GW+IFEAFE++TKV SGGYTSLDDVT VPP RRDKMETFFLGE
Sbjct: 541 SLFVLYRITKDTKYRDQGWQIFEAFEKYTKVKSGGYTSLDDVTEVPPHRRDKMETFFLGE 600

Query: 601 TLKYLYLLFGDSSTIPLDKYVFNTEAHPLPIEGN 631
           TLKYLYLLFGD S IPLDK+VFNTEAHPLPI  N
Sbjct: 601 TLKYLYLLFGDDSVIPLDKFVFNTEAHPLPIRRN 623

BLAST of Cla97C01G024530 vs. TAIR 10
Match: AT1G51590.1 (alpha-mannosidase 1 )

HSP 1 Score: 357.8 bits (917), Expect = 1.8e-98
Identity = 222/496 (44.76%), Postives = 297/496 (59.88%), Query Frame = 0

Query: 136 RQQKVKDAFIHAWSGYKRYAMGYDELMPLSQQGTDGLGGLGATVVDALDTAMIMGASDIV 195
           R+QKVK+A IHAWS Y++YA G DEL P ++ GTD  GGLGAT+VD+LDT  IMG  +  
Sbjct: 97  RRQKVKEAMIHAWSSYEKYAWGKDELQPRTKDGTDSFGGLGATMVDSLDTLYIMGLDEQF 156

Query: 196 SEAGSWVEKHLLDRIKEKGQVNLFETTIRVLGGLLSAYHLRAGEYGKTFENEGPKPAVYL 255
            +A  WV   L     +    ++FETTIRV+GGLLSAY L            G K  ++L
Sbjct: 157 QKAREWVASSL--DFDKDYDASMFETTIRVVGGLLSAYDL-----------SGDK--MFL 216

Query: 256 EIAKNLADRLLSAFTSSPTAIPLCDVVLKDSSAHA---APGGSSSTSEVSTLQLEFNYLS 315
           E AK++ADRLL A+ ++PT IP   + L++ +AH    A GG S  ++  T QLEF  LS
Sbjct: 217 EKAKDIADRLLPAW-NTPTGIPYNIINLRNGNAHNPSWAAGGDSILADSGTEQLEFIALS 276

Query: 316 TVSGDPKYSIAAMKVLEHM-KNLPKVEGLVPIYISPHSGQFSGETIRLGSRGDSYYEYLL 375
             +GDPKY     KV+  + KN P  +GL+PIYI+P +   S  T   G+ GDS+YEYLL
Sbjct: 277 QRTGDPKYQQKVEKVITELNKNFP-ADGLLPIYINPDNANPSYSTTTFGAMGDSFYEYLL 336

Query: 376 KVWLQKRTRENNNFTYLHDMYEEAMKGVKHLLVRKSVPKELVFVGELPYGSGSAISPKMD 435
           KVW+Q    + +      DM+E++MKG+   LV+KS P    ++ E    +G+ +  KMD
Sbjct: 337 KVWVQ--GNKTSAVKPYRDMWEKSMKGLLS-LVKKSTPSSFTYICE---KNGNNLIDKMD 396

Query: 436 HLVCFLSGTLALGATKGITKEEAMSKSLLSFDDLENLNLAEDLAKTCYEMYEVTSTGLAP 495
            L CF  G LALGA+ G   +E          + + L+LA +LA TCY  Y+ T T LA 
Sbjct: 397 ELACFAPGMLALGAS-GYGPDE----------EKKFLSLAGELAWTCYNFYQSTPTKLAG 456

Query: 496 EIVYFHTEEYSEQGFDGGNKNSKYIDDIIIKPLDRHNLMRPETVESLFVLYRITGNQKYR 555
           E  +F   +    G                      N++RPETVESLF L+R+TGN+ Y+
Sbjct: 457 ENYFFTAGQDMSVG-------------------TSWNILRPETVESLFYLWRLTGNKTYQ 516

Query: 556 AWGWEIFEAFERHTKVSSGGYTSLDDVTTVPPRRRDKMETFFLGETLKYLYLLFGDSSTI 615
            WGW IF+AFE++++V S GY  L DV T    + +KM++FFL ETLKYLYLLF  SS I
Sbjct: 517 EWGWNIFQAFEKNSRVES-GYVGLKDVNT--GAKDNKMQSFFLAETLKYLYLLFSPSSVI 536

Query: 616 PLDKYVFNTEAHPLPI 628
            LD++VFNTEAHPL I
Sbjct: 577 SLDEWVFNTEAHPLKI 536

BLAST of Cla97C01G024530 vs. TAIR 10
Match: AT3G21160.1 (alpha-mannosidase 2 )

HSP 1 Score: 350.9 bits (899), Expect = 2.1e-96
Identity = 218/496 (43.95%), Postives = 295/496 (59.48%), Query Frame = 0

Query: 136 RQQKVKDAFIHAWSGYKRYAMGYDELMPLSQQGTDGLGGLGATVVDALDTAMIMGASDIV 195
           R Q+VK+A +HAWS Y++YA G DEL P ++ G D  GGLGAT++DALDT  IMG  +  
Sbjct: 98  RMQRVKEAMVHAWSSYEKYAWGQDELQPQTKDGVDSFGGLGATMIDALDTLYIMGLDEQF 157

Query: 196 SEAGSWVEKHLLDRIKEKGQVNLFETTIRVLGGLLSAYHLRAGEYGKTFENEGPKPAVYL 255
            +A  WV    LD  K+    ++FETTIRV+GGLLSAY L            G K  ++L
Sbjct: 158 QKAREWVASS-LDFDKDYA-ASMFETTIRVVGGLLSAYDL-----------SGDK--IFL 217

Query: 256 EIAKNLADRLLSAFTSSPTAIPLCDVVLKDSSAH--AAPGGSSSTSEVSTLQLEFNYLST 315
           E A ++ADRLL A+  + + IP   + LK  +AH     GG S  ++  T QLEF  LS 
Sbjct: 218 EKAMDIADRLLPAW-DTQSGIPYNIINLKHGNAHNPTWAGGDSILADSGTEQLEFIALSQ 277

Query: 316 VSGDPKYSIAAMKVLEHM-KNLPKVEGLVPIYISPHSGQFSGETIRLGSRGDSYYEYLLK 375
            +GDPKY     KV+  + KN P  +GL+PIYI+P +   S  TI  G+ GDS+YEYLLK
Sbjct: 278 RTGDPKYQQKVEKVISVLNKNFP-ADGLLPIYINPDTANPSQSTITFGAMGDSFYEYLLK 337

Query: 376 VWLQKRTRENNNFTYLHDMYEEAMKGVKHLLVRKSVPKELVFVGELPYGSGSAISPKMDH 435
           VW+     + +   +  DM+E++M G+   LV+KS P    ++ E    SG+++  KMD 
Sbjct: 338 VWV--FGNKTSAVKHYRDMWEKSMNGLLS-LVKKSTPLSFTYICE---KSGNSLIDKMDE 397

Query: 436 LVCFLSGTLALGATKGITKEEAMSKSLLSFDDLENLNLAEDLAKTCYEMYEVTSTGLAPE 495
           L CF  G LALGA+     + A  K  L+        LAE+LA TCY  Y+ T T LA E
Sbjct: 398 LACFAPGMLALGASG--YSDPAEGKKFLT--------LAEELAWTCYNFYQSTPTKLAGE 457

Query: 496 IVYFHTEEYSEQGFDGGNKNSKYIDDIIIKPLDRHNLMRPETVESLFVLYRITGNQKYRA 555
             +F++      G                      N++RPETVESLF L+R+TGN+ Y+ 
Sbjct: 458 NYFFNSGSDMSVG-------------------TSWNILRPETVESLFYLWRLTGNKTYQE 517

Query: 556 WGWEIFEAFERHTKVSSGGYTSLDDVTTVPPRRRDKMETFFLGETLKYLYLLFGDSSTIP 615
           WGW IFEAFE+++++ S GY  L DV T    + +KM++FFL ETLKYLYLLF  ++ IP
Sbjct: 518 WGWNIFEAFEKNSRIES-GYVGLKDVNT--GVKDNKMQSFFLAETLKYLYLLFSPTTVIP 538

Query: 616 LDKYVFNTEAHPLPIE 629
           LD++VFNTEAHPL I+
Sbjct: 578 LDEWVFNTEAHPLKIK 538

BLAST of Cla97C01G024530 vs. TAIR 10
Match: AT1G51590.2 (alpha-mannosidase 1 )

HSP 1 Score: 345.1 bits (884), Expect = 1.2e-94
Identity = 216/487 (44.35%), Postives = 289/487 (59.34%), Query Frame = 0

Query: 145 IHAWSGYKRYAMGYDELMPLSQQGTDGLGGLGATVVDALDTAMIMGASDIVSEAGSWVEK 204
           IHAWS Y++YA G DEL P ++ GTD  GGLGAT+VD+LDT  IMG  +   +A  WV  
Sbjct: 2   IHAWSSYEKYAWGKDELQPRTKDGTDSFGGLGATMVDSLDTLYIMGLDEQFQKAREWVAS 61

Query: 205 HLLDRIKEKGQVNLFETTIRVLGGLLSAYHLRAGEYGKTFENEGPKPAVYLEIAKNLADR 264
            L     +    ++FETTIRV+GGLLSAY L            G K  ++LE AK++ADR
Sbjct: 62  SL--DFDKDYDASMFETTIRVVGGLLSAYDL-----------SGDK--MFLEKAKDIADR 121

Query: 265 LLSAFTSSPTAIPLCDVVLKDSSAHA---APGGSSSTSEVSTLQLEFNYLSTVSGDPKYS 324
           LL A+ ++PT IP   + L++ +AH    A GG S  ++  T QLEF  LS  +GDPKY 
Sbjct: 122 LLPAW-NTPTGIPYNIINLRNGNAHNPSWAAGGDSILADSGTEQLEFIALSQRTGDPKYQ 181

Query: 325 IAAMKVLEHM-KNLPKVEGLVPIYISPHSGQFSGETIRLGSRGDSYYEYLLKVWLQKRTR 384
               KV+  + KN P  +GL+PIYI+P +   S  T   G+ GDS+YEYLLKVW+Q    
Sbjct: 182 QKVEKVITELNKNFP-ADGLLPIYINPDNANPSYSTTTFGAMGDSFYEYLLKVWVQ--GN 241

Query: 385 ENNNFTYLHDMYEEAMKGVKHLLVRKSVPKELVFVGELPYGSGSAISPKMDHLVCFLSGT 444
           + +      DM+E++MKG+   LV+KS P    ++ E    +G+ +  KMD L CF  G 
Sbjct: 242 KTSAVKPYRDMWEKSMKGLLS-LVKKSTPSSFTYICE---KNGNNLIDKMDELACFAPGM 301

Query: 445 LALGATKGITKEEAMSKSLLSFDDLENLNLAEDLAKTCYEMYEVTSTGLAPEIVYFHTEE 504
           LALGA+ G   +E          + + L+LA +LA TCY  Y+ T T LA E  +F   +
Sbjct: 302 LALGAS-GYGPDE----------EKKFLSLAGELAWTCYNFYQSTPTKLAGENYFFTAGQ 361

Query: 505 YSEQGFDGGNKNSKYIDDIIIKPLDRHNLMRPETVESLFVLYRITGNQKYRAWGWEIFEA 564
               G                      N++RPETVESLF L+R+TGN+ Y+ WGW IF+A
Sbjct: 362 DMSVG-------------------TSWNILRPETVESLFYLWRLTGNKTYQEWGWNIFQA 421

Query: 565 FERHTKVSSGGYTSLDDVTTVPPRRRDKMETFFLGETLKYLYLLFGDSSTIPLDKYVFNT 624
           FE++++V S GY  L DV T    + +KM++FFL ETLKYLYLLF  SS I LD++VFNT
Sbjct: 422 FEKNSRVES-GYVGLKDVNT--GAKDNKMQSFFLAETLKYLYLLFSPSSVISLDEWVFNT 432

Query: 625 EAHPLPI 628
           EAHPL I
Sbjct: 482 EAHPLKI 432

BLAST of Cla97C01G024530 vs. TAIR 10
Match: AT5G43710.1 (Glycosyl hydrolase family 47 protein )

HSP 1 Score: 171.8 bits (434), Expect = 1.8e-42
Identity = 154/510 (30.20%), Postives = 235/510 (46.08%), Query Frame = 0

Query: 137 QQKVKDAFIHAWSGYKRYAMGYDELMPLSQQGTDGLGGLGATVVDALDTAMIMGASDIVS 196
           + +V+  F HA+ GY   A   DEL PLS QG D LGG   T++D+LDT  ++G  +  +
Sbjct: 41  RDEVRGMFYHAFDGYMNNAFPLDELRPLSCQGEDTLGGYALTLIDSLDTLALLGDRERFT 100

Query: 197 EAGSWVEKHLLDRIKEKGQVNLFETTIRVLGGLLSAYHLRAGEYG-----KTFENEGPKP 256
            +  W+ K+L   I +   V++FETTIRVLGGLLSA HL A +Y       ++ NE    
Sbjct: 101 SSVEWIGKNLQFNINK--TVSVFETTIRVLGGLLSA-HLIASDYATGMRIPSYNNE---- 160

Query: 257 AVYLEIAKNLADRLLSAFTSSPTAIPLCDVVLKDSSAHAAPGGSSSTSEVSTLQLEFNYL 316
              L +A+NLA R+L AF  +PT IP   V L            +ST+   TL LEF  L
Sbjct: 161 --LLVLAENLARRMLPAF-DTPTGIPFGSVNLM-YGVDKHESKITSTAGGGTLSLEFGVL 220

Query: 317 STVSGDPKYSIAAMKVLEHMKNLPKVEGLVPIYISPHSGQFSGETIRLGSRGDSYYEYLL 376
           S ++ DP +   A   +  +        LV  +I+  +G+++ +   +G+  DS+YEYLL
Sbjct: 221 SRLTNDPVFEQVAKNAVRGLWARRSNLDLVGAHINVFTGEWTQKDAGIGTSIDSFYEYLL 280

Query: 377 KVWLQKRTRENNNFTYLHDMYEEAMKGVKHLLVRKSVPKELVFVGELPYGSGSAISPKMD 436
           K ++     E     YL+ +++EA +     L      K+  +V E+   S + + P  +
Sbjct: 281 KAYILFGDEE-----YLY-IFQEAYRSAMQYL-----HKDPWYV-EVNMDSAAIVWPVFN 340

Query: 437 HLVCFLSGTLALGATKGITKEEAMSKSLLSFDDLENLNLAEDLAKTCYEMYEVTSTG--L 496
            L  F  G   L                    D++          + ++ Y  T  G  L
Sbjct: 341 SLQAFWPGLQVLAG------------------DVDPAIRTHTAFFSVWKRYGFTPEGFNL 400

Query: 497 APEIVYFHTEEYSEQGFDGGNKNSKYIDDIIIKPLDRHNLMRPETVESLFVLYRITGNQK 556
           A   V +  + Y                            +RPE +ES + LY+ T + +
Sbjct: 401 ATLSVQYGQKSYP---------------------------LRPELIESTYWLYKATRDPR 460

Query: 557 YRAWGWEIFEAFERHTKVSSGGYTSLDDVTTVPPRRRDKMETFFLGETLKYLYLLFG--- 616
           Y   G +   + +   K    GY  + DV     ++ D ME+FFL ET+KYL+LLF    
Sbjct: 461 YLDAGRDFVASLQYGAKCPC-GYCHITDVEL--HKQEDHMESFFLAETVKYLWLLFDLAV 479

Query: 617 DSSTIPLD---KYVFNTEAHPLPIEGNIQI 634
           DS  +  +   KY+F+TE H LPI   I +
Sbjct: 521 DSDNLVDNGPYKYIFSTEGHLLPITPQISL 479

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
XP_038882608.10.0e+0096.69mannosyl-oligosaccharide 1,2-alpha-mannosidase MNS3 isoform X1 [Benincasa hispid... [more]
XP_008439639.10.0e+0094.64PREDICTED: mannosyl-oligosaccharide 1,2-alpha-mannosidase MNS3 [Cucumis melo][more]
XP_004134582.10.0e+0093.69mannosyl-oligosaccharide 1,2-alpha-mannosidase MNS3 [Cucumis sativus] >KGN49392.... [more]
XP_038882609.10.0e+0096.55mannosyl-oligosaccharide 1,2-alpha-mannosidase MNS3 isoform X2 [Benincasa hispid... [more]
XP_023517941.10.0e+0092.42mannosyl-oligosaccharide 1,2-alpha-mannosidase MNS3-like [Cucurbita pepo subsp. ... [more]
Match NameE-valueIdentityDescription
Q93Y378.9e-26571.77Mannosyl-oligosaccharide 1,2-alpha-mannosidase MNS3 OS=Arabidopsis thaliana OX=3... [more]
Q9UKM71.3e-11145.98Endoplasmic reticulum mannosyl-oligosaccharide 1,2-alpha-mannosidase OS=Homo sap... [more]
Q8J0Q09.7e-11046.76Mannosyl-oligosaccharide 1,2-alpha-mannosidase OS=Candida albicans OX=5476 GN=MN... [more]
B2GUY01.5e-10745.27Endoplasmic reticulum mannosyl-oligosaccharide 1,2-alpha-mannosidase OS=Rattus n... [more]
A2AJ151.0e-10644.87Endoplasmic reticulum mannosyl-oligosaccharide 1,2-alpha-mannosidase OS=Mus musc... [more]
Match NameE-valueIdentityDescription
A0A1S3AZ690.0e+0094.64alpha-1,2-Mannosidase OS=Cucumis melo OX=3656 GN=LOC103484372 PE=3 SV=1[more]
A0A0A0KLY90.0e+0093.69alpha-1,2-Mannosidase OS=Cucumis sativus OX=3659 GN=Csa_6G523340 PE=3 SV=1[more]
A0A6J1KW660.0e+0092.26alpha-1,2-Mannosidase OS=Cucurbita maxima OX=3661 GN=LOC111496964 PE=3 SV=1[more]
A0A6J1ELX60.0e+0092.26alpha-1,2-Mannosidase OS=Cucurbita moschata OX=3662 GN=LOC111433756 PE=3 SV=1[more]
A0A5A7UEF90.0e+0094.56alpha-1,2-Mannosidase OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold25... [more]
Match NameE-valueIdentityDescription
AT1G30000.16.3e-26671.77alpha-mannosidase 3 [more]
AT1G51590.11.8e-9844.76alpha-mannosidase 1 [more]
AT3G21160.12.1e-9643.95alpha-mannosidase 2 [more]
AT1G51590.21.2e-9444.35alpha-mannosidase 1 [more]
AT5G43710.11.8e-4230.20Glycosyl hydrolase family 47 protein [more]
InterPro
Analysis Name: InterPro Annotations of Watermelon (97103) v2.5
Date Performed: 2022-01-31
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR001382Glycoside hydrolase family 47PRINTSPR00747GLYHDRLASE47coord: 215..233
score: 63.66
coord: 529..553
score: 47.43
coord: 360..377
score: 58.2
coord: 143..163
score: 50.57
coord: 176..190
score: 62.22
coord: 268..287
score: 24.52
coord: 429..445
score: 52.66
coord: 591..611
score: 58.28
IPR001382Glycoside hydrolase family 47PFAMPF01532Glyco_hydro_47coord: 143..627
e-value: 6.2E-158
score: 526.5
IPR012341Six-hairpin glycosidase-like superfamilyGENE3D1.50.10.10coord: 134..633
e-value: 6.1E-173
score: 578.0
NoneNo IPR availablePANTHERPTHR11742MANNOSYL-OLIGOSACCHARIDE ALPHA-1,2-MANNOSIDASE-RELATEDcoord: 14..628
NoneNo IPR availablePANTHERPTHR11742:SF88ALPHA-1,2-MANNOSIDASEcoord: 14..628
IPR036026Seven-hairpin glycosidasesSUPERFAMILY48225Seven-hairpin glycosidasescoord: 133..630

Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
Cla97C01G024530.1Cla97C01G024530.1mRNA


GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
biological_process GO:0005975 carbohydrate metabolic process
biological_process GO:0006491 N-glycan processing
biological_process GO:0030433 ubiquitin-dependent ERAD pathway
cellular_component GO:0005783 endoplasmic reticulum
cellular_component GO:0016021 integral component of membrane
cellular_component GO:0016020 membrane
molecular_function GO:0005509 calcium ion binding
molecular_function GO:0004571 mannosyl-oligosaccharide 1,2-alpha-mannosidase activity