Cla97C01G010645 (gene) Watermelon (97103) v2.5

BLAST of Cla97C01G010645 vs. NCBI nr
Match: XP_038893739.1 (tRNA (guanine(9)-N1)-methyltransferase [Benincasa hispida])

HSP 1 Score: 598.6 bits (1542), Expect = 3.3e-167
Identity = 317/337 (94.07%), Postives = 323/337 (95.85%), Query Frame = 0

Query: 1   MEAMEEGQSAKMDPPSSPSTCNPPEPPLSKNARKKLLKQQRYEAKKAEKKLQAKEQKKKD 60
           MEA+EE QSAKMDPP SP   NPPEPPLSKNA+KKLLKQQRYEAKKAEKKLQAKEQKKKD
Sbjct: 1   MEAVEEDQSAKMDPPPSPFAFNPPEPPLSKNAKKKLLKQQRYEAKKAEKKLQAKEQKKKD 60

Query: 61  IERKRKEWEETLASVTEEERSKLIESRRSLRKERMGKRSEEREKKIERLNMAKDCGQNIV 120
           IERKRKEWE+TLASVTEEERSKLIESRRSLRKERMGKRSEEREKKIERLN AKDCGQNIV
Sbjct: 61  IERKRKEWEDTLASVTEEERSKLIESRRSLRKERMGKRSEEREKKIERLNRAKDCGQNIV 120

Query: 121 IDLEFSHLMTPSEIHSLVQQIMYCYAVNGRCPSPGHLWLTGCNGEMETQLQRLPGFDKWI 180
           IDLEFSHLMTPSEIHSLVQQIMYCYAVNGRCPSPGHLWLTGCNGEMETQLQRLPGFDKWI
Sbjct: 121 IDLEFSHLMTPSEIHSLVQQIMYCYAVNGRCPSPGHLWLTGCNGEMETQLQRLPGFDKWI 180

Query: 181 IEKEHRSYIDALQDQKENLVYLTADAETVLDDLDLKKIYIIGGLVDRNRWKGITMKKAQE 240
           IEKEHRSYIDALQDQKENLVYLTADAETVLDDLD KKIYIIGGLVDRNRWKGITMKKA E
Sbjct: 181 IEKEHRSYIDALQDQKENLVYLTADAETVLDDLDPKKIYIIGGLVDRNRWKGITMKKAHE 240

Query: 241 QGIQTAKLPIGSYLKMSSSQVLTVNQVIEILLKYLETRDWKASFFQVVPQRKRCEADSNS 300
           QGIQTAKLPIGSYLKMSSSQVLTVNQVIEILLKYLETRDWKASFFQVVPQRKRC ADSNS
Sbjct: 241 QGIQTAKLPIGSYLKMSSSQVLTVNQVIEILLKYLETRDWKASFFQVVPQRKRCGADSNS 300

Query: 301 DHPVDGEEN--GDGDGDNEHETKKTKCDKVSSSNHTL 336
           DHPVD EEN  GDGDGD+EHE KKTKCD+VSSS+ TL
Sbjct: 301 DHPVDEEENGDGDGDGDDEHEMKKTKCDEVSSSDDTL 337

BLAST of Cla97C01G010645 vs. NCBI nr
Match: KAG6583717.1 (hypothetical protein SDJN03_19649, partial [Cucurbita argyrosperma subsp. sororia])

HSP 1 Score: 588.6 bits (1516), Expect = 3.5e-164
Identity = 309/334 (92.51%), Postives = 319/334 (95.51%), Query Frame = 0

Query: 1   MEAMEEGQSAKMDPPSSPSTCNPPEPPLSKNARKKLLKQQRYEAKKAEKKLQAKEQKKKD 60
           MEA EE QSAKMDPP SPS CNPPEPPLSKNARKKLLKQQRYEAKKAEKK Q KEQKKKD
Sbjct: 1   MEAREEDQSAKMDPPCSPSGCNPPEPPLSKNARKKLLKQQRYEAKKAEKKAQEKEQKKKD 60

Query: 61  IERKRKEWEETLASVTEEERSKLIESRRSLRKERMGKRSEEREKKIERLNMAKDCGQNIV 120
           IERKRKEWEETLASVTEEER+KLIESR+SLRKERMGKRSEE+EKKIERLN AKDCGQ IV
Sbjct: 61  IERKRKEWEETLASVTEEERAKLIESRKSLRKERMGKRSEEKEKKIERLNRAKDCGQKIV 120

Query: 121 IDLEFSHLMTPSEIHSLVQQIMYCYAVNGRCPSPGHLWLTGCNGEMETQLQRLPGFDKWI 180
           IDLEFS LMTPSEIHSLVQQIMYCYAVNGRCPSPGHLWLTGCNGEMETQLQRLPGFDKWI
Sbjct: 121 IDLEFSDLMTPSEIHSLVQQIMYCYAVNGRCPSPGHLWLTGCNGEMETQLQRLPGFDKWI 180

Query: 181 IEKEHRSYIDALQDQKENLVYLTADAETVLDDLDLKKIYIIGGLVDRNRWKGITMKKAQE 240
           IE+EHRSYIDALQDQKENLVYLTAD+ETVLDDLDLKKIYIIGGLVDRNRWKGITMKKAQE
Sbjct: 181 IEREHRSYIDALQDQKENLVYLTADSETVLDDLDLKKIYIIGGLVDRNRWKGITMKKAQE 240

Query: 241 QGIQTAKLPIGSYLKMSSSQVLTVNQVIEILLKYLETRDWKASFFQVVPQRKRCEADSNS 300
           QGIQTAKLPIGSYLKMSSSQVLTVNQV+EILLKY+ETRDWKASFFQVVPQRKRCEA+ NS
Sbjct: 241 QGIQTAKLPIGSYLKMSSSQVLTVNQVLEILLKYIETRDWKASFFQVVPQRKRCEAECNS 300

Query: 301 DHPVDGEENGDGDGDNEHETKKTKCDKVSSSNHT 335
           DH VDGEEN  GD D+E ETKKTKCD+VSSSNHT
Sbjct: 301 DHAVDGEEN--GDRDDEQETKKTKCDEVSSSNHT 332

BLAST of Cla97C01G010645 vs. NCBI nr
Match: XP_023520714.1 (tRNA (guanine(9)-N1)-methyltransferase [Cucurbita pepo subsp. pepo])

HSP 1 Score: 587.8 bits (1514), Expect = 5.9e-164
Identity = 309/334 (92.51%), Postives = 319/334 (95.51%), Query Frame = 0

Query: 1   MEAMEEGQSAKMDPPSSPSTCNPPEPPLSKNARKKLLKQQRYEAKKAEKKLQAKEQKKKD 60
           MEA+EE QSAKMDPP SPS CNPPEPPLSKNARKKLLKQQRYEAKKAEKK Q KE KKKD
Sbjct: 1   MEAIEEDQSAKMDPPCSPSGCNPPEPPLSKNARKKLLKQQRYEAKKAEKKAQEKELKKKD 60

Query: 61  IERKRKEWEETLASVTEEERSKLIESRRSLRKERMGKRSEEREKKIERLNMAKDCGQNIV 120
           IERKRKEWEETLASVTEEER+KLIESR+SLRKERMGKRSEE+EKKIERLN AKDCGQ IV
Sbjct: 61  IERKRKEWEETLASVTEEERAKLIESRKSLRKERMGKRSEEKEKKIERLNRAKDCGQKIV 120

Query: 121 IDLEFSHLMTPSEIHSLVQQIMYCYAVNGRCPSPGHLWLTGCNGEMETQLQRLPGFDKWI 180
           IDLEFS LMTPSEIHSLVQQIMYCYAVNGRCPSPGHLWLTGCNGEMETQLQRLPGFDKWI
Sbjct: 121 IDLEFSDLMTPSEIHSLVQQIMYCYAVNGRCPSPGHLWLTGCNGEMETQLQRLPGFDKWI 180

Query: 181 IEKEHRSYIDALQDQKENLVYLTADAETVLDDLDLKKIYIIGGLVDRNRWKGITMKKAQE 240
           IEKEHRSYIDALQDQKENLVYLTAD+ETVLDDLDLKKIYIIGGLVDRNRWKGITMKKAQE
Sbjct: 181 IEKEHRSYIDALQDQKENLVYLTADSETVLDDLDLKKIYIIGGLVDRNRWKGITMKKAQE 240

Query: 241 QGIQTAKLPIGSYLKMSSSQVLTVNQVIEILLKYLETRDWKASFFQVVPQRKRCEADSNS 300
           QGIQTAKLPIGSYLKMSSSQVLTVNQV+EILLKY+ETRDWKASFFQVVPQRKRCEA+ NS
Sbjct: 241 QGIQTAKLPIGSYLKMSSSQVLTVNQVLEILLKYIETRDWKASFFQVVPQRKRCEAECNS 300

Query: 301 DHPVDGEENGDGDGDNEHETKKTKCDKVSSSNHT 335
           DH VDGEEN  GD D+E ETKKTKCD+VSSSNHT
Sbjct: 301 DHAVDGEEN--GDRDDEQETKKTKCDEVSSSNHT 332

BLAST of Cla97C01G010645 vs. NCBI nr
Match: XP_022973151.1 (tRNA (guanine(9)-N1)-methyltransferase [Cucurbita maxima])

HSP 1 Score: 583.6 bits (1503), Expect = 1.1e-162
Identity = 306/334 (91.62%), Postives = 318/334 (95.21%), Query Frame = 0

Query: 1   MEAMEEGQSAKMDPPSSPSTCNPPEPPLSKNARKKLLKQQRYEAKKAEKKLQAKEQKKKD 60
           MEA+EE QSAKMDPP SPS CNPPEPPLSKNARKKLLKQQRYEAKKAEKK Q KEQKKKD
Sbjct: 1   MEAIEEDQSAKMDPPCSPSGCNPPEPPLSKNARKKLLKQQRYEAKKAEKKAQEKEQKKKD 60

Query: 61  IERKRKEWEETLASVTEEERSKLIESRRSLRKERMGKRSEEREKKIERLNMAKDCGQNIV 120
           IERKRKEWE+TLASVTEEER+KLIESRRSLRKERMGKRSEE+EKKIERLN AKDCGQ I+
Sbjct: 61  IERKRKEWEDTLASVTEEERAKLIESRRSLRKERMGKRSEEKEKKIERLNRAKDCGQKII 120

Query: 121 IDLEFSHLMTPSEIHSLVQQIMYCYAVNGRCPSPGHLWLTGCNGEMETQLQRLPGFDKWI 180
           IDLEFS LMTPSEIHSLVQQIMYCYAVNGRCPSPGHLWLTGCNGEMETQLQRLPGFDKWI
Sbjct: 121 IDLEFSDLMTPSEIHSLVQQIMYCYAVNGRCPSPGHLWLTGCNGEMETQLQRLPGFDKWI 180

Query: 181 IEKEHRSYIDALQDQKENLVYLTADAETVLDDLDLKKIYIIGGLVDRNRWKGITMKKAQE 240
           IEKEHRSYIDALQDQKENLVYLTAD+ETVLDDLDLKKIYIIGGLVDRNRWKGITMKKAQE
Sbjct: 181 IEKEHRSYIDALQDQKENLVYLTADSETVLDDLDLKKIYIIGGLVDRNRWKGITMKKAQE 240

Query: 241 QGIQTAKLPIGSYLKMSSSQVLTVNQVIEILLKYLETRDWKASFFQVVPQRKRCEADSNS 300
           QGIQTAKLPIGSYLKMSSSQVLTVNQV+EILLKY+ETRDWKASFFQVVPQRKRCEA+ NS
Sbjct: 241 QGIQTAKLPIGSYLKMSSSQVLTVNQVLEILLKYIETRDWKASFFQVVPQRKRCEAECNS 300

Query: 301 DHPVDGEENGDGDGDNEHETKKTKCDKVSSSNHT 335
           DH VDGEEN  GD D+E E+KK KC +VSSSNHT
Sbjct: 301 DHAVDGEEN--GDRDDEQESKKIKCGEVSSSNHT 332

BLAST of Cla97C01G010645 vs. NCBI nr
Match: XP_022927615.1 (tRNA (guanine(9)-N1)-methyltransferase [Cucurbita moschata])

HSP 1 Score: 583.2 bits (1502), Expect = 1.5e-162
Identity = 306/334 (91.62%), Postives = 317/334 (94.91%), Query Frame = 0

Query: 1   MEAMEEGQSAKMDPPSSPSTCNPPEPPLSKNARKKLLKQQRYEAKKAEKKLQAKEQKKKD 60
           MEA EE QSAKMDPP SPS CNPPEPPLSKNARKKLLKQQRYEAKKAEKK Q KEQKKKD
Sbjct: 1   MEAREEDQSAKMDPPCSPSGCNPPEPPLSKNARKKLLKQQRYEAKKAEKKAQEKEQKKKD 60

Query: 61  IERKRKEWEETLASVTEEERSKLIESRRSLRKERMGKRSEEREKKIERLNMAKDCGQNIV 120
           IERKRKEWEETLASVTEEER+KLIESR+SLRKERMGKRSEE+EKKIERLN AKDCGQ IV
Sbjct: 61  IERKRKEWEETLASVTEEERAKLIESRKSLRKERMGKRSEEKEKKIERLNRAKDCGQKIV 120

Query: 121 IDLEFSHLMTPSEIHSLVQQIMYCYAVNGRCPSPGHLWLTGCNGEMETQLQRLPGFDKWI 180
           IDLEFS LMTPSEIHSLVQQIMYCYAVNGRCPSPGHLWLTGCNGEMETQLQRLPGFDKWI
Sbjct: 121 IDLEFSDLMTPSEIHSLVQQIMYCYAVNGRCPSPGHLWLTGCNGEMETQLQRLPGFDKWI 180

Query: 181 IEKEHRSYIDALQDQKENLVYLTADAETVLDDLDLKKIYIIGGLVDRNRWKGITMKKAQE 240
           IEKEHRSYIDALQD KENLVYLTAD+ETVLDDLDLKKIYIIGGLVDRNRWKGITM KAQE
Sbjct: 181 IEKEHRSYIDALQDHKENLVYLTADSETVLDDLDLKKIYIIGGLVDRNRWKGITMNKAQE 240

Query: 241 QGIQTAKLPIGSYLKMSSSQVLTVNQVIEILLKYLETRDWKASFFQVVPQRKRCEADSNS 300
           QGIQTAKLPIGSYLKMSSSQVLTVNQV+EILL+Y+ETRDWKASFFQVVPQRKRCEA+ NS
Sbjct: 241 QGIQTAKLPIGSYLKMSSSQVLTVNQVLEILLRYIETRDWKASFFQVVPQRKRCEAECNS 300

Query: 301 DHPVDGEENGDGDGDNEHETKKTKCDKVSSSNHT 335
           DH VDG+EN  GD D+E ETKKTKCD+VSSSNHT
Sbjct: 301 DHAVDGKEN--GDRDDEQETKKTKCDEVSSSNHT 332

BLAST of Cla97C01G010645 vs. ExPASy Swiss-Prot
Match: Q8TBZ6 (tRNA methyltransferase 10 homolog A OS=Homo sapiens OX=9606 GN=TRMT10A PE=1 SV=1)

HSP 1 Score: 156.8 bits (395), Expect = 4.4e-37
Identity = 115/324 (35.49%), Postives = 179/324 (55.25%), Query Frame = 0

Query: 27  PLSKNARKKLLKQQRYEAKKAEKKLQAKEQKKKDIERKRKEWEETLASVTEEERSKLIES 86
           P+SK   KKL+KQ+++E ++     + ++QK+K+ +RKRK+ E                 
Sbjct: 39  PISKRQMKKLIKQKQWEEQR-----ELRKQKRKE-KRKRKKLE----------------- 98

Query: 87  RRSLRKERMGKRSEEREKKIERLNMAKDCGQNIVIDLEFSHLMTPSEIHSLVQQIMYCYA 146
               R+ +M   S+  ++K  R ++       ++ID  F HLM   +I  L +QI  CYA
Sbjct: 99  ----RQCQMEPNSDGHDRKRVRRDVVHST-LRLIIDCSFDHLMVLKDIKKLHKQIQRCYA 158

Query: 147 VNGRCPSPGHLWLTGCNGEMETQL-QRLPGFDKW---IIEKEHRSYIDALQDQKENLVYL 206
            N R   P   +LT   G+++  + +   G+  W    I+ EH S +     +KE+L+YL
Sbjct: 159 ENRRALHPVQFYLTSHGGQLKKNMDENDKGWVNWKDIHIKPEHYSEL----IKKEDLIYL 218

Query: 207 TADAETVLDDLDLKKIYIIGGLVDRNRWKGITMKKAQEQGIQTAKLPIGSYLKMSSSQVL 266
           T+D+  +L +LD  K Y+IGGLVD N  KG+T K+A + GI  A+LP+G+++KM+S +VL
Sbjct: 219 TSDSPNILKELDESKAYVIGGLVDHNHHKGLTYKQASDYGINHAQLPLGNFVKMNSRKVL 278

Query: 267 TVNQVIEILLKYLETRDWKASFFQVVPQR-------KRCEADSNSDHPVDGEENGDGDGD 326
            VN V EI+L+YLETRDW+ +FF ++PQR       K CE+ S+ +  V  EE G     
Sbjct: 279 AVNHVFEIILEYLETRDWQEAFFTILPQRKGAVPTDKACESASHDNQSVRMEEGGSDSDS 330

Query: 327 NEHETKKTKCD-----KVSSSNHT 335
           +E E  + + D     K    NHT
Sbjct: 339 SEEEYSRNELDSPHEEKQDKENHT 330

BLAST of Cla97C01G010645 vs. ExPASy Swiss-Prot
Match: O14214 (tRNA (guanine(9)-N1)-methyltransferase OS=Schizosaccharomyces pombe (strain 972 / ATCC 24843) OX=284812 GN=trm10 PE=1 SV=1)

HSP 1 Score: 148.7 bits (374), Expect = 1.2e-34
Identity = 107/301 (35.55%), Postives = 166/301 (55.15%), Query Frame = 0

Query: 25  EPPLSKNARKKLLKQQRYEAKKAEKKLQAKEQKKKDIERKRKEWEETLASVTEEERSKLI 84
           +P LSK+A K+L +QQ ++A + EK+ + + +KK+               + +EER + I
Sbjct: 29  QPVLSKSALKRLKRQQEWDAGR-EKRAEMRREKKR---------------LRKEERKRKI 88

Query: 85  ESRRSLRKERMGKRSEEREKKIERLNMAKDCGQNIVIDLEFSHLMTPSEIHSLVQQIMYC 144
           E+   ++           +KK  RL         IV+D  F  LM   EI+SL QQ+  C
Sbjct: 89  EAGEVVKS----------QKKRIRLGKVVPSSIRIVLDCAFDDLMNDKEINSLCQQVTRC 148

Query: 145 YAVNGRCPSPGHLWLTGCNGEMETQLQ-RLPG-FDKW-IIEKEHRSYIDALQDQKENLVY 204
           ++ N     P  L+ T   G ++T+    L G  + W       +SY++  + QKE LVY
Sbjct: 149 HSANRTALHPVELFATNFGGRLKTRQDFVLKGQQNNWKRYNPTTKSYLEEFESQKEKLVY 208

Query: 205 LTADAETVLDDLDLKKIYIIGGLVDRNRWKGITMKKAQEQGIQTAKLPIGSYLKMSSSQV 264
           L+AD++  + +LD  KIYIIG +VD+NR+K +   KA EQGI+TAKLPI  Y+K++  ++
Sbjct: 209 LSADSDNTITELDEDKIYIIGAIVDKNRYKNLCQNKASEQGIKTAKLPIDEYIKITDRKI 268

Query: 265 LTVNQVIEILLKYLETRDWKASFFQVVPQRKRCEADSNSDHPVDGEENGDGDGDNEHETK 323
           LTVNQV EIL  +LE RDW+ +F +V+P+RK     S+    V  E+       ++ E +
Sbjct: 269 LTVNQVFEILSLWLEYRDWEKAFMEVIPKRKGILLKSDESFDV-SEDTRSQSNQSDSELE 302

BLAST of Cla97C01G010645 vs. ExPASy Swiss-Prot
Match: Q8C1Z8 (tRNA methyltransferase 10 homolog A OS=Mus musculus OX=10090 GN=Trmt10a PE=2 SV=2)

HSP 1 Score: 147.9 bits (372), Expect = 2.0e-34
Identity = 110/320 (34.38%), Postives = 171/320 (53.44%), Query Frame = 0

Query: 27  PLSKNARKKLLKQQRYEAKKAEKKLQAKEQKKKDIERKRKEWEETLASVTEEERSKLIES 86
           P+SK   KKL+KQ+++E ++ ++K + KE++K                            
Sbjct: 39  PISKRQLKKLMKQKQWEEQREQRKEKRKEKRK---------------------------- 98

Query: 87  RRSLRKERMGKRSEEREKKIERLNMAKDCGQNIVIDLEFSHLMTPSEIHSLVQQIMYCYA 146
           R+ L + ++   S+  ++K  R ++A+     +VID  F  LM   +I  L +QI  CYA
Sbjct: 99  RKKLERRQLESNSDGNDRKRVRRDVARS-SLRLVIDCSFDDLMVLKDIKKLHKQIQRCYA 158

Query: 147 VNGRCPSPGHLWLTGCNGEMETQL-QRLPGFDKW---IIEKEHRSYIDALQDQKENLVYL 206
            N R   P   +LT   G+++  + +   G+  W    I+ EH S +     +KE+LVYL
Sbjct: 159 ENRRASHPVQFYLTSHGGQLKKNMDENDQGWVNWKDIHIKSEHYSEL----IKKEDLVYL 218

Query: 207 TADAETVLDDLDLKKIYIIGGLVDRNRWKGITMKKAQEQGIQTAKLPIGSYLKMSSSQVL 266
           T+D+  VL DLD  K Y+IGGLVD +  KG+T K+A   GI+ A+LP+  ++KM+S +VL
Sbjct: 219 TSDSPNVLKDLDESKAYVIGGLVDHSHHKGLTFKQATSYGIEHAQLPLADFVKMNSRKVL 278

Query: 267 TVNQVIEILLKYLETRDWKASFFQVVPQR-------KRCEADSNSDHPV-----DGEENG 326
            VN V EI+L++LETRDW+ +FF ++PQR       K CE+ S  DH       D    G
Sbjct: 279 AVNHVFEIILEFLETRDWQEAFFTILPQRKGAVPAHKACES-SPQDHQSLPEGWDSASEG 324

Query: 327 DGDGDNEHETKKTKCDKVSS 331
           +   DN    +K +  + SS
Sbjct: 339 ESCRDNPDSPQKDEQGQQSS 324

BLAST of Cla97C01G010645 vs. ExPASy Swiss-Prot
Match: Q3MHI8 (tRNA methyltransferase 10 homolog A OS=Bos taurus OX=9913 GN=TRMT10A PE=2 SV=1)

HSP 1 Score: 147.1 bits (370), Expect = 3.5e-34
Identity = 115/327 (35.17%), Postives = 176/327 (53.82%), Query Frame = 0

Query: 27  PLSKNARKKLLKQQRYEAKKAEKKLQAKEQKKKDIERKRKEWEETLASVTEEERSKLIES 86
           P+SK   KKL+KQ+++E ++     + ++QK+K+ +RKRK+ E      +  + S     
Sbjct: 39  PISKRQMKKLMKQKQWEEQR-----ELRKQKRKE-KRKRKQLERQCQPESNSDGSDRKRI 98

Query: 87  RRSLRKERMGKRSEEREKKIERLNMAKDCGQNIVIDLEFSHLMTPSEIHSLVQQIMYCYA 146
           RR +    +                       ++ID  F  LM   +I  L +QI  CYA
Sbjct: 99  RRDVVHSPL----------------------RLIIDCSFDSLMVLKDIKKLHKQIQRCYA 158

Query: 147 VNGRCPSPGHLWLTGCNGEMETQL-QRLPGFDKW---IIEKEHRSYIDALQDQKENLVYL 206
            N R   P   +LT   G+++  + +   G+  W    I+ EH S       QKE+L+YL
Sbjct: 159 ENRRALHPVQFYLTSHGGQLKKNMDENDKGWVNWKDIHIKPEHYSEF----IQKEDLIYL 218

Query: 207 TADAETVLDDLDLKKIYIIGGLVDRNRWKGITMKKAQEQGIQTAKLPIGSYLKMSSSQVL 266
           T+D+  +L +LD  K Y+IGGLVD N  KG+T K+A + GI  A+LP+G+++KM+S +VL
Sbjct: 219 TSDSPNILKELDESKAYVIGGLVDHNHHKGLTYKQASDHGIDHAQLPLGNFVKMNSRKVL 278

Query: 267 TVNQVIEILLKYLETRDWKASFFQVVPQRK-------RCEADSNSDH--PVDGEENGD-G 326
            VN V EI+L+YLETRDW+ +FF ++PQRK        CE+ S+      V+G  N D  
Sbjct: 279 AVNHVFEIILEYLETRDWQEAFFTILPQRKGAVPTDQACESCSHDKKFARVEGGLNSDSS 333

Query: 327 DGDNEHETKKT----KCDKVSSSNHTL 336
           + +N HE   T    K DK +S+  T+
Sbjct: 339 EEENRHELDSTHEEEKQDKENSTESTV 333

BLAST of Cla97C01G010645 vs. ExPASy Swiss-Prot
Match: Q66JJ4 (tRNA methyltransferase 10 homolog A OS=Xenopus tropicalis OX=8364 GN=trmt10a PE=2 SV=1)

HSP 1 Score: 138.7 bits (348), Expect = 1.2e-31
Identity = 122/360 (33.89%), Postives = 185/360 (51.39%), Query Frame = 0

Query: 1   MEAMEEGQSAKMDPPSSPSTCNPPEPPLSKNARKKLLKQQRYEAKKAEKKLQ---AKEQK 60
           +EA+ E     +DP       N  +   S++A      Q+   A ++E K Q   +K Q 
Sbjct: 3   LEAVTEPGKCSIDP-------NTKDLLASQHAGNNTPLQENSSAPRSECKAQDAMSKRQM 62

Query: 61  KKDIERKRKEWEETLASVTEEERSKLIESRRSLRKERMGKRSEEREKKIER--------- 120
           KK +  K+K+WE+              E R+  RKE+  KR  ER+ + E          
Sbjct: 63  KKFL--KQKQWEDQR------------ELRKQKRKEKRQKRKLERQAQAEHNIDANSRKR 122

Query: 121 -LNMAKDCGQNIVIDLEFSHLMTPSEIHSLVQQIMYCYAVNGRCPSPGHLWLTGCNGEME 180
             +  +     ++ID  F  LM   ++  L +QI  CYA N R   P  L+LT   G+++
Sbjct: 123 FRHEVQPSALRLIIDCSFDDLMALRDVKKLNKQIRRCYAENRRAVHPVQLYLTSHGGQLK 182

Query: 181 TQLQRLPGFDK-WI------IEKEHRSYIDALQDQKENLVYLTADAETVLDDLDLKKIYI 240
           + +     +DK WI      I+ EH  Y D +  +KE+LVYLT+D+  VL +LD  K YI
Sbjct: 183 SNMDE---YDKGWINWKDIHIKPEH--YKDLI--KKEDLVYLTSDSPEVLSELDETKAYI 242

Query: 241 IGGLVDRNRWKGITMKKAQEQGIQTAKLPIGSYLKMSSSQVLTVNQVIEILLKYLETRDW 300
           IGGLVD N  KGIT KKA E GI  A+LP+G+++KM++ +VL VN V EI+L +LE ++W
Sbjct: 243 IGGLVDHNHHKGITYKKALELGISHAQLPLGNFVKMNTRKVLAVNHVFEIILAFLEKKEW 302

Query: 301 KASFFQVVPQRKRC--------EADSNSDHPVDGEENGDGDGDNEHET--KKTKCDKVSS 331
           K +FF V+PQRK          + +  +    DGE++      +E  T     +C++ +S
Sbjct: 303 KEAFFSVLPQRKGAIPLTETGEQPECRASEQEDGEDSDSDSSIDESATIQPSVRCEEQNS 334

BLAST of Cla97C01G010645 vs. ExPASy TrEMBL
Match: A0A6J1I7W5 (tRNA (guanine(9)-N(1))-methyltransferase OS=Cucurbita maxima OX=3661 GN=LOC111471663 PE=4 SV=1)

HSP 1 Score: 583.6 bits (1503), Expect = 5.4e-163
Identity = 306/334 (91.62%), Postives = 318/334 (95.21%), Query Frame = 0

Query: 1   MEAMEEGQSAKMDPPSSPSTCNPPEPPLSKNARKKLLKQQRYEAKKAEKKLQAKEQKKKD 60
           MEA+EE QSAKMDPP SPS CNPPEPPLSKNARKKLLKQQRYEAKKAEKK Q KEQKKKD
Sbjct: 1   MEAIEEDQSAKMDPPCSPSGCNPPEPPLSKNARKKLLKQQRYEAKKAEKKAQEKEQKKKD 60

Query: 61  IERKRKEWEETLASVTEEERSKLIESRRSLRKERMGKRSEEREKKIERLNMAKDCGQNIV 120
           IERKRKEWE+TLASVTEEER+KLIESRRSLRKERMGKRSEE+EKKIERLN AKDCGQ I+
Sbjct: 61  IERKRKEWEDTLASVTEEERAKLIESRRSLRKERMGKRSEEKEKKIERLNRAKDCGQKII 120

Query: 121 IDLEFSHLMTPSEIHSLVQQIMYCYAVNGRCPSPGHLWLTGCNGEMETQLQRLPGFDKWI 180
           IDLEFS LMTPSEIHSLVQQIMYCYAVNGRCPSPGHLWLTGCNGEMETQLQRLPGFDKWI
Sbjct: 121 IDLEFSDLMTPSEIHSLVQQIMYCYAVNGRCPSPGHLWLTGCNGEMETQLQRLPGFDKWI 180

Query: 181 IEKEHRSYIDALQDQKENLVYLTADAETVLDDLDLKKIYIIGGLVDRNRWKGITMKKAQE 240
           IEKEHRSYIDALQDQKENLVYLTAD+ETVLDDLDLKKIYIIGGLVDRNRWKGITMKKAQE
Sbjct: 181 IEKEHRSYIDALQDQKENLVYLTADSETVLDDLDLKKIYIIGGLVDRNRWKGITMKKAQE 240

Query: 241 QGIQTAKLPIGSYLKMSSSQVLTVNQVIEILLKYLETRDWKASFFQVVPQRKRCEADSNS 300
           QGIQTAKLPIGSYLKMSSSQVLTVNQV+EILLKY+ETRDWKASFFQVVPQRKRCEA+ NS
Sbjct: 241 QGIQTAKLPIGSYLKMSSSQVLTVNQVLEILLKYIETRDWKASFFQVVPQRKRCEAECNS 300

Query: 301 DHPVDGEENGDGDGDNEHETKKTKCDKVSSSNHT 335
           DH VDGEEN  GD D+E E+KK KC +VSSSNHT
Sbjct: 301 DHAVDGEEN--GDRDDEQESKKIKCGEVSSSNHT 332

BLAST of Cla97C01G010645 vs. ExPASy TrEMBL
Match: A0A6J1EPG7 (tRNA (guanine(9)-N(1))-methyltransferase OS=Cucurbita moschata OX=3662 GN=LOC111434383 PE=4 SV=1)

HSP 1 Score: 583.2 bits (1502), Expect = 7.0e-163
Identity = 306/334 (91.62%), Postives = 317/334 (94.91%), Query Frame = 0

Query: 1   MEAMEEGQSAKMDPPSSPSTCNPPEPPLSKNARKKLLKQQRYEAKKAEKKLQAKEQKKKD 60
           MEA EE QSAKMDPP SPS CNPPEPPLSKNARKKLLKQQRYEAKKAEKK Q KEQKKKD
Sbjct: 1   MEAREEDQSAKMDPPCSPSGCNPPEPPLSKNARKKLLKQQRYEAKKAEKKAQEKEQKKKD 60

Query: 61  IERKRKEWEETLASVTEEERSKLIESRRSLRKERMGKRSEEREKKIERLNMAKDCGQNIV 120
           IERKRKEWEETLASVTEEER+KLIESR+SLRKERMGKRSEE+EKKIERLN AKDCGQ IV
Sbjct: 61  IERKRKEWEETLASVTEEERAKLIESRKSLRKERMGKRSEEKEKKIERLNRAKDCGQKIV 120

Query: 121 IDLEFSHLMTPSEIHSLVQQIMYCYAVNGRCPSPGHLWLTGCNGEMETQLQRLPGFDKWI 180
           IDLEFS LMTPSEIHSLVQQIMYCYAVNGRCPSPGHLWLTGCNGEMETQLQRLPGFDKWI
Sbjct: 121 IDLEFSDLMTPSEIHSLVQQIMYCYAVNGRCPSPGHLWLTGCNGEMETQLQRLPGFDKWI 180

Query: 181 IEKEHRSYIDALQDQKENLVYLTADAETVLDDLDLKKIYIIGGLVDRNRWKGITMKKAQE 240
           IEKEHRSYIDALQD KENLVYLTAD+ETVLDDLDLKKIYIIGGLVDRNRWKGITM KAQE
Sbjct: 181 IEKEHRSYIDALQDHKENLVYLTADSETVLDDLDLKKIYIIGGLVDRNRWKGITMNKAQE 240

Query: 241 QGIQTAKLPIGSYLKMSSSQVLTVNQVIEILLKYLETRDWKASFFQVVPQRKRCEADSNS 300
           QGIQTAKLPIGSYLKMSSSQVLTVNQV+EILL+Y+ETRDWKASFFQVVPQRKRCEA+ NS
Sbjct: 241 QGIQTAKLPIGSYLKMSSSQVLTVNQVLEILLRYIETRDWKASFFQVVPQRKRCEAECNS 300

Query: 301 DHPVDGEENGDGDGDNEHETKKTKCDKVSSSNHT 335
           DH VDG+EN  GD D+E ETKKTKCD+VSSSNHT
Sbjct: 301 DHAVDGKEN--GDRDDEQETKKTKCDEVSSSNHT 332

BLAST of Cla97C01G010645 vs. ExPASy TrEMBL
Match: A0A0A0LYJ3 (SAM-dependent MTase TRM10-type domain-containing protein OS=Cucumis sativus OX=3659 GN=Csa_1G173210 PE=4 SV=1)

HSP 1 Score: 566.6 bits (1459), Expect = 6.8e-158
Identity = 299/335 (89.25%), Postives = 316/335 (94.33%), Query Frame = 0

Query: 1   MEAMEEGQSAKMDPPSSPSTCNPPEPPLSKNARKKLLKQQRYEAKKAEKKLQAKEQKKKD 60
           MEA+EE  + KMDPP S S CNPPEPPLSKNARKKLLKQQRYEAKKA+KKLQAKEQKKKD
Sbjct: 1   MEAVEESHNPKMDPPCSSSACNPPEPPLSKNARKKLLKQQRYEAKKADKKLQAKEQKKKD 60

Query: 61  IERKRKEWEETLASVTEEERSKLIESRRSLRKERMGKRSEEREKKIERLNMAKDCGQNIV 120
           IERKRKEWEE LASVTEEER+KLIESRR+LRKERMGKRSE+REKKIERL+ A+D GQNIV
Sbjct: 61  IERKRKEWEEKLASVTEEERAKLIESRRNLRKERMGKRSEDREKKIERLSTARDYGQNIV 120

Query: 121 IDLEFSHLMTPSEIHSLVQQIMYCYAVNGRCPSPGHLWLTGCNGEMETQLQRLPGFDKWI 180
           IDLEFSHLMTP EIHSLVQQIMYCYAVNGRCPSP HLWLTGCNGEMETQLQRLPGFDKWI
Sbjct: 121 IDLEFSHLMTPWEIHSLVQQIMYCYAVNGRCPSPCHLWLTGCNGEMETQLQRLPGFDKWI 180

Query: 181 IEKEHRSYIDALQDQKENLVYLTADAETVLDDLDLKKIYIIGGLVDRNRWKGITMKKAQE 240
           IEKE RSYIDALQ+QKENLVYLTAD+ETVLDDLDLKKIYI+GGLVDRNRWKGITMKKAQE
Sbjct: 181 IEKECRSYIDALQEQKENLVYLTADSETVLDDLDLKKIYIVGGLVDRNRWKGITMKKAQE 240

Query: 241 QGIQTAKLPIGSYLKMSSSQVLTVNQVIEILLKYLETRDWKASFFQVVPQRKRCEADSNS 300
           Q IQTAKLPIGSYLKMSSSQVLTVNQVIEILLKYLETRDWK SFF+VVPQRKRC  DSNS
Sbjct: 241 QEIQTAKLPIGSYLKMSSSQVLTVNQVIEILLKYLETRDWKDSFFEVVPQRKRCGGDSNS 300

Query: 301 DHPVDGEENGDGDGDNEHETKKTKCDKVSSSNHTL 336
           DHPVDGEEN  GDGDN++ETKKTKCD+VSSS++ L
Sbjct: 301 DHPVDGEEN--GDGDNKNETKKTKCDEVSSSDNNL 333

BLAST of Cla97C01G010645 vs. ExPASy TrEMBL
Match: A0A5D3CW30 (tRNA (Guanine(9)-N1)-methyltransferase isoform X1 OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold477G00280 PE=4 SV=1)

HSP 1 Score: 563.9 bits (1452), Expect = 4.4e-157
Identity = 296/329 (89.97%), Postives = 310/329 (94.22%), Query Frame = 0

Query: 1   MEAMEEGQSAKMDPPSSPSTCNPPEPPLSKNARKKLLKQQRYEAKKAEKKLQAKEQKKKD 60
           ME +E G S K+DPP SPS CNPPEP LSKNARKKLLKQQRYEAKKA+KKLQAKEQKKKD
Sbjct: 1   MEDVEGGHSPKIDPPCSPSACNPPEPSLSKNARKKLLKQQRYEAKKADKKLQAKEQKKKD 60

Query: 61  IERKRKEWEETLASVTEEERSKLIESRRSLRKERMGKRSEEREKKIERLNMAKDCGQNIV 120
           IERKRKEWEE LA VTEEER+KLIESRR+LRKERMGKRSE+REKKIERLN AKDCGQNIV
Sbjct: 61  IERKRKEWEEKLACVTEEERAKLIESRRNLRKERMGKRSEDREKKIERLNTAKDCGQNIV 120

Query: 121 IDLEFSHLMTPSEIHSLVQQIMYCYAVNGRCPSPGHLWLTGCNGEMETQLQRLPGFDKWI 180
           IDLEFSHLMTPSEIHSLVQQIMYCYAVNGRCPSP HLWLTGCNGEM +QLQRLPGFDKWI
Sbjct: 121 IDLEFSHLMTPSEIHSLVQQIMYCYAVNGRCPSPCHLWLTGCNGEMGSQLQRLPGFDKWI 180

Query: 181 IEKEHRSYIDALQDQKENLVYLTADAETVLDDLDLKKIYIIGGLVDRNRWKGITMKKAQE 240
           IEKE RSYIDALQ+QKENLVYLTAD+ETVLDDLDLKKIYI+GGLVDRNRWKGIT+KKAQE
Sbjct: 181 IEKERRSYIDALQEQKENLVYLTADSETVLDDLDLKKIYIVGGLVDRNRWKGITLKKAQE 240

Query: 241 QGIQTAKLPIGSYLKMSSSQVLTVNQVIEILLKYLETRDWKASFFQVVPQRKRCEADSNS 300
           Q IQTA+LPIGSYLKMSSSQVLTVNQVIEILLKYLETRDWK SFFQVVPQRKRC ADSNS
Sbjct: 241 QEIQTARLPIGSYLKMSSSQVLTVNQVIEILLKYLETRDWKDSFFQVVPQRKRCGADSNS 300

Query: 301 DHPVDGEENGDGDGDNEHETKKTKCDKVS 330
           DHPVDGEEN  GDGDN+ ETKKTKCD+VS
Sbjct: 301 DHPVDGEEN--GDGDNKQETKKTKCDEVS 327

BLAST of Cla97C01G010645 vs. ExPASy TrEMBL
Match: A0A1S3CL37 (tRNA (Guanine(9)-N1)-methyltransferase isoform X1 OS=Cucumis melo OX=3656 GN=LOC103502202 PE=4 SV=1)

HSP 1 Score: 563.9 bits (1452), Expect = 4.4e-157
Identity = 296/329 (89.97%), Postives = 310/329 (94.22%), Query Frame = 0

Query: 1   MEAMEEGQSAKMDPPSSPSTCNPPEPPLSKNARKKLLKQQRYEAKKAEKKLQAKEQKKKD 60
           ME +E G S K+DPP SPS CNPPEP LSKNARKKLLKQQRYEAKKA+KKLQAKEQKKKD
Sbjct: 1   MEDVEGGHSPKIDPPCSPSACNPPEPSLSKNARKKLLKQQRYEAKKADKKLQAKEQKKKD 60

Query: 61  IERKRKEWEETLASVTEEERSKLIESRRSLRKERMGKRSEEREKKIERLNMAKDCGQNIV 120
           IERKRKEWEE LA VTEEER+KLIESRR+LRKERMGKRSE+REKKIERLN AKDCGQNIV
Sbjct: 61  IERKRKEWEEKLACVTEEERAKLIESRRNLRKERMGKRSEDREKKIERLNTAKDCGQNIV 120

Query: 121 IDLEFSHLMTPSEIHSLVQQIMYCYAVNGRCPSPGHLWLTGCNGEMETQLQRLPGFDKWI 180
           IDLEFSHLMTPSEIHSLVQQIMYCYAVNGRCPSP HLWLTGCNGEM +QLQRLPGFDKWI
Sbjct: 121 IDLEFSHLMTPSEIHSLVQQIMYCYAVNGRCPSPCHLWLTGCNGEMGSQLQRLPGFDKWI 180

Query: 181 IEKEHRSYIDALQDQKENLVYLTADAETVLDDLDLKKIYIIGGLVDRNRWKGITMKKAQE 240
           IEKE RSYIDALQ+QKENLVYLTAD+ETVLDDLDLKKIYI+GGLVDRNRWKGIT+KKAQE
Sbjct: 181 IEKERRSYIDALQEQKENLVYLTADSETVLDDLDLKKIYIVGGLVDRNRWKGITLKKAQE 240

Query: 241 QGIQTAKLPIGSYLKMSSSQVLTVNQVIEILLKYLETRDWKASFFQVVPQRKRCEADSNS 300
           Q IQTA+LPIGSYLKMSSSQVLTVNQVIEILLKYLETRDWK SFFQVVPQRKRC ADSNS
Sbjct: 241 QEIQTARLPIGSYLKMSSSQVLTVNQVIEILLKYLETRDWKDSFFQVVPQRKRCGADSNS 300

Query: 301 DHPVDGEENGDGDGDNEHETKKTKCDKVS 330
           DHPVDGEEN  GDGDN+ ETKKTKCD+VS
Sbjct: 301 DHPVDGEEN--GDGDNKQETKKTKCDEVS 327

BLAST of Cla97C01G010645 vs. TAIR 10
Match: AT5G47680.1 (FUNCTIONS IN: molecular_function unknown; INVOLVED IN: biological_process unknown; LOCATED IN: cellular_component unknown; EXPRESSED IN: 23 plant structures; EXPRESSED DURING: 13 growth stages; CONTAINS InterPro DOMAIN/s: tRNA (guanine-N1-)-methyltransferase (InterPro:IPR016009), tRNA (guanine-N(1)-)-methyltransferase, metazoa (InterPro:IPR016653), tRNA (guanine-N1-)-methyltransferase, eukaryotic (InterPro:IPR007356); Has 1807 Blast hits to 1807 proteins in 277 species: Archae - 0; Bacteria - 0; Metazoa - 736; Fungi - 347; Plants - 385; Viruses - 0; Other Eukaryotes - 339 (source: NCBI BLink). )

HSP 1 Score: 396.7 bits (1018), Expect = 1.8e-110
Identity = 224/322 (69.57%), Postives = 259/322 (80.43%), Query Frame = 0

Query: 14  PPSSPSTCNPPEPPLSKNARKKLLKQQRYEAKKAEKKLQAKEQKKKDIERKRKEWEETLA 73
           P S P+  N   PPLSKNA+KK LKQQRYEAKKAEKK Q KE K+K+ ERK KEWEETLA
Sbjct: 16  PESEPAPVN-VSPPLSKNAQKKQLKQQRYEAKKAEKKAQEKEHKRKEGERKLKEWEETLA 75

Query: 74  SVTEEERSKLIESRRSLRKERMGKRSEEREKKIERLNMAKDCGQNIVIDLEFSHLMTPSE 133
           + TEEER KLIESR+SLRKERM KRSEE+EKKIERLN AK+ GQ IV+D++F+HLM+ SE
Sbjct: 76  NATEEERLKLIESRKSLRKERMEKRSEEKEKKIERLNQAKEIGQKIVVDVDFAHLMSESE 135

Query: 134 IHSLVQQIMYCYAVNGRCPSPGHLWLTGCNGEMETQLQRLPGFDKWIIEKEHRSYIDALQ 193
           I SLVQQIMYCYAVNGR  SP HLWLTG  G+M T+L +LPGF+KW IEKE R YI+A+ 
Sbjct: 136 ISSLVQQIMYCYAVNGRSTSPCHLWLTGVQGKMSTELDKLPGFEKWFIEKESRCYIEAMA 195

Query: 194 DQKENLVYLTADAETVLDDLDLKKIYIIGGLVDRNRWKGITMKKAQEQGIQTAKLPIGSY 253
           DQK+NLVYLTAD+ETVLDDLD K IYIIGGLVDRNR+KGITM KAQEQGI+TAKLPIG Y
Sbjct: 196 DQKDNLVYLTADSETVLDDLDPKHIYIIGGLVDRNRFKGITMTKAQEQGIKTAKLPIGEY 255

Query: 254 LKMSSSQVLTVNQVIEILLKYLETRDWKASFFQVVPQRKRCEADSNSDHPVDGEENGDGD 313
           +KMSSSQVLTVNQV+EIL+K+LETRDWK +FF V+PQRKR   D     PVD  +     
Sbjct: 256 MKMSSSQVLTVNQVLEILVKFLETRDWKTAFFTVIPQRKRTGLD-----PVDCSKL--EH 315

Query: 314 GDNEHETKKTKCDKVSSSNHTL 336
              EH+ K T+ ++ S+  H L
Sbjct: 316 ISEEHQEKDTEANE-SADKHDL 328