Cla97C01G008675 (gene) Watermelon (97103) v2.5

Overview
NameCla97C01G008675
Typegene
OrganismCitrullus lanatus subsp. vulgaris cv. 97103 (Watermelon (97103) v2.5)
DescriptionPropionate-CoA ligase
LocationCla97Chr01: 9227701 .. 9230808 (+)
RNA-Seq ExpressionCla97C01G008675
SyntenyCla97C01G008675
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: exonfive_prime_UTRCDSpolypeptidethree_prime_UTR
Hold the cursor over a type above to highlight its positions in the sequence below.
TTCCATGATTGGTGTGCAACACTCCTCCTCCCTCTTTCTTCTTCTTCATCTTCCTCCTTTCTTATTAAATCTCATCTTCTTCTACTCGATCTTCAGTTTTCCTAGTCCTAGCCCTCTCCTTAGTCGCCATTGCGGTTCGCAGCGCCTTCCATCACCGATAATTTCAGTTCCATTTTTCTCTCAAGTTTAAATGTCACTTTTTACTTCGTTGATTTTTACCCGAACATCCTATAAAGTTTGTTTAAAATTTTGTGTCTATTTAATCTCTAAAATTTTATAAATTATTGCAAAATACAAATTTTAAAAAAATAAATAGCAAAATCTTATTAATGAGTACATACACTTTTATGAATTTCCATCAATATGTTGACATTTTCATCGATATCTTCGTATTTTCATGTTTTCAATATCAATACGAAGGGCGAATCGATATTTTTATTATATCATGGAAAATCCACTAAACATAAAAGAATAAGCATCAAAATGTCATTAATATAAATAATAGACATATTTACTTATTTGAAAATAATAGAATATTTATTTACTAATTTAAATATATTTTTTATTTTTATAATTTTTCTTTAAAAAATCATCCATATTTCCATCAAAATTTCTCTAAAATTAGAAACTCCATATTTCATCTATATTTTAAACTTTGCTTTTTGATTAATGAAATAAACATAACCCCCGAAAAGAATACACATAACTGTCAAGAGACTTTTATTATTATTATTTTTTGAGTTTCACAATTTACAGATAGATAGTTGAATCTTTGGATTGGCATTTAAAAAAGTTCAAAGAAAAACCACAAAAATACTTCAAAATTATAAATATAAATTTACATATGTTGTAATTATTAAAAAAAAAAATGCAGATTTTCAAATTTAGATGTAAGAATTTAAACAATTTTTATCTATAAAAAAAGTGTGTTCTTCTTCTTATCATCATCTTCTTCGAAGTTGTTGAACTTTCTTTTATAATTGCAGAGGGAGAAAATTGAGCAACAATAAGATAACAGAGCATAAACAATTTCTATGGAAAATTTGAAGCCACGCCCTGCTAATTCCTCTCCCCTCACTCCAATCGGCTTCATCGAAAGAACCGCCCTCATTTATGGCGATTCTCCCTCGATCATCTACAACTCCACCACTTACACCTGGTCGGAAACCCATCGCCGATGCCTTCAACTCGCCTCTTCCCTCTCTTCCATTGGCATCCAAAGAGGCCACGTCGTCTCCGTTATCGCTCCCAACACTCCGGCCATGTACGAACTCCACTTCGCCGTTCCGATGGTTGGTGCCGTCCTTAATACCATCAATCTCCGCCTCGACCCTCGAACCATCTCCGTCCTCCTCCGCCACAGCGAAACCAAACTCATCTTCGTCGATCAAGCCTCTTGTGCTCTAATCTCCGACGCTTTGGCCTTGTTCCCTTCGGAGACGAAACGGCCGTTACTCGTCCTCATTGCCGACGACACTGCGGTACAAGATTCGTCATTGTCATCCTCTGTTTTGATTCGCAACGATTTTGTCGATTCGTACGAGGAAATGGTTTGGAAAGGGGATTCTAATTTCGAATGGATAAGACCGGTGAGTGAATGGGATCCGATGGTTTTGAATTACACTTCTGGAACAACGTCGTCGCCGAAAGGAGTGGTTCATTGTCACAGGGGAATTTTCACGGTGGCACTCGATTCGCTTGTGGAATGGGGAGTTCCTAAGCAATCAGTTTATTTATGGACTTTACCAATGTTTCATGCCAATGGGTGGAGTTTTCCTTGGGGAATTGCTGCCGTAGGTGGAACAAATGTTTGTATTCGAAAATTCGATGCATCATTAATTTTTTCCCTGATTCGTCGCCATCATGTGACACACTTATGTGGAGCTCCGGTCGTACTCAATATGCTAACGAACTCTCCTGACAATCGACCACTTGATCGACCGGTCAAGATTCTGACTGCCGGAGCACCACCGCCTGCGGCAGTTCTGCTTCGAATTGAATCATTGGGGTTTGTTGTCACTCATGGGTATGGATTAACTGAGAGTGGTGGGACGATCATCAGCTGTTCATGGAAGTCGGAGTGGAACCGGCTGACGCAAACAGAGCAAGCACGGCTGAAGGCGAGGCAGGGGGTACGGATGCTAGCGGTTGAGGCGGCGGATGTGTTAGATCCAAAGACTAGAAAAAGTGTGAAAAGAGACGGAGCTTCCATAGGAGAGATTGTTGTGAGAGGCGGATCGATAATGCTTGGGTATTTGAAAGATCCAGAAGCGACAGCGAAAGCGATGACCGACGACGGGTGGTTCTTCACCGGAGATGTGGGGGTGATGCATCCAGATGGGTATATAGAGATAAAGGACAGATCGAAGGATGTGATTATTAGTGGAGGAGAGAATTTGAGCAGCGTTGAGGTGGAATCAGTTCTGTACATGAATCCGGTGGTGAATGAAGTGGCAGTAGTGGCACGACCGGACGAGTTCTGGGGAGAGACACCGTGTGCTTTTGTGAACTTGAAAAAAGGATTGACTCGACAACCGACGGAGGAAGAGATTATTGAGTTTTGTAAAGGGAAGCTGCCACGATTTATGGTGCCTAAGACGGTGGTCTTCACCACGGAGCTTCCAAAGACATCAACCGGAAAAATTCTGAAGTCTGTGCTCAGAGAAATGGCCAAGTCAATGGGCCCCACACCAAAAAGTAGATTGTAAAAAAAAAAAAAAAAAAAATTCACAAATCATTTATATTCATATTACCAAAAATATCATCCATCTCCAAAATAAATAAATAAATATTATGGTGTTTCTTATTATAAATATTCTAATATGGGTTGGGTTTTAAATAGATGCCCATGTTTAGTGTTAAAAAAACCAAATGGCATTCTCACACTATCCCATGTGTCTTATACTTAAACATCATTGGTGTCCATGTAATTTCACCTCTTAATTGCCAAATTTCCTAGAAATAATGACGTTTGGTGGATTTGGAAGAGGTGTGCATATTGATAAAAATCCAAATATATTAGAGAAGATTTCATAGATTTCATAAATTTCATATTGTCGAATTTCTTGTTATTTTGTTAATTATTTGTTTTAATATTATAATTCTTCG

mRNA sequence

TTCCATGATTGGTGTGCAACACTCCTCCTCCCTCTTTCTTCTTCTTCATCTTCCTCCTTTCTTATTAAATCTCATCTTCTTCTACTCGATCTTCAGTTTTCCTAGTCCTAGCCCTCTCCTTAGTCGCCATTGCGGTTCGCAGCGCCTTCCATCACCGATAATTTCAAGGGAGAAAATTGAGCAACAATAAGATAACAGAGCATAAACAATTTCTATGGAAAATTTGAAGCCACGCCCTGCTAATTCCTCTCCCCTCACTCCAATCGGCTTCATCGAAAGAACCGCCCTCATTTATGGCGATTCTCCCTCGATCATCTACAACTCCACCACTTACACCTGGTCGGAAACCCATCGCCGATGCCTTCAACTCGCCTCTTCCCTCTCTTCCATTGGCATCCAAAGAGGCCACGTCGTCTCCGTTATCGCTCCCAACACTCCGGCCATGTACGAACTCCACTTCGCCGTTCCGATGGTTGGTGCCGTCCTTAATACCATCAATCTCCGCCTCGACCCTCGAACCATCTCCGTCCTCCTCCGCCACAGCGAAACCAAACTCATCTTCGTCGATCAAGCCTCTTGTGCTCTAATCTCCGACGCTTTGGCCTTGTTCCCTTCGGAGACGAAACGGCCGTTACTCGTCCTCATTGCCGACGACACTGCGGTACAAGATTCGTCATTGTCATCCTCTGTTTTGATTCGCAACGATTTTGTCGATTCGTACGAGGAAATGGTTTGGAAAGGGGATTCTAATTTCGAATGGATAAGACCGGTGAGTGAATGGGATCCGATGGTTTTGAATTACACTTCTGGAACAACGTCGTCGCCGAAAGGAGTGGTTCATTGTCACAGGGGAATTTTCACGGTGGCACTCGATTCGCTTGTGGAATGGGGAGTTCCTAAGCAATCAGTTTATTTATGGACTTTACCAATGTTTCATGCCAATGGGTGGAGTTTTCCTTGGGGAATTGCTGCCGTAGGTGGAACAAATGTTTGTATTCGAAAATTCGATGCATCATTAATTTTTTCCCTGATTCGTCGCCATCATGTGACACACTTATGTGGAGCTCCGGTCGTACTCAATATGCTAACGAACTCTCCTGACAATCGACCACTTGATCGACCGGTCAAGATTCTGACTGCCGGAGCACCACCGCCTGCGGCAGTTCTGCTTCGAATTGAATCATTGGGGTTTGTTGTCACTCATGGGTATGGATTAACTGAGAGTGGTGGGACGATCATCAGCTGTTCATGGAAGTCGGAGTGGAACCGGCTGACGCAAACAGAGCAAGCACGGCTGAAGGCGAGGCAGGGGGTACGGATGCTAGCGGTTGAGGCGGCGGATGTGTTAGATCCAAAGACTAGAAAAAGTGTGAAAAGAGACGGAGCTTCCATAGGAGAGATTGTTGTGAGAGGCGGATCGATAATGCTTGGGTATTTGAAAGATCCAGAAGCGACAGCGAAAGCGATGACCGACGACGGGTGGTTCTTCACCGGAGATGTGGGGGTGATGCATCCAGATGGGTATATAGAGATAAAGGACAGATCGAAGGATGTGATTATTAGTGGAGGAGAGAATTTGAGCAGCGTTGAGGTGGAATCAGTTCTGTACATGAATCCGGTGGTGAATGAAGTGGCAGTAGTGGCACGACCGGACGAGTTCTGGGGAGAGACACCGTGTGCTTTTGTGAACTTGAAAAAAGGATTGACTCGACAACCGACGGAGGAAGAGATTATTGAGTTTTGTAAAGGGAAGCTGCCACGATTTATGGTGCCTAAGACGGTGGTCTTCACCACGGAGCTTCCAAAGACATCAACCGGAAAAATTCTGAAGTCTGTGCTCAGAGAAATGGCCAAGTCAATGGGCCCCACACCAAAAAGTAGATTGTAAAAAAAAAAAAAAAAAAAATTCACAAATCATTTATATTCATATTACCAAAAATATCATCCATCTCCAAAATAAATAAATAAATATTATGGTGTTTCTTATTATAAATATTCTAATATGGGTTGGGTTTTAAATAGATGCCCATGTTTAGTGTTAAAAAAACCAAATGGCATTCTCACACTATCCCATGTGTCTTATACTTAAACATCATTGGTGTCCATGTAATTTCACCTCTTAATTGCCAAATTTCCTAGAAATAATGACGTTTGGTGGATTTGGAAGAGGTGTGCATATTGATAAAAATCCAAATATATTAGAGAAGATTTCATAGATTTCATAAATTTCATATTGTCGAATTTCTTGTTATTTTGTTAATTATTTGTTTTAATATTATAATTCTTCG

Coding sequence (CDS)

ATGGAAAATTTGAAGCCACGCCCTGCTAATTCCTCTCCCCTCACTCCAATCGGCTTCATCGAAAGAACCGCCCTCATTTATGGCGATTCTCCCTCGATCATCTACAACTCCACCACTTACACCTGGTCGGAAACCCATCGCCGATGCCTTCAACTCGCCTCTTCCCTCTCTTCCATTGGCATCCAAAGAGGCCACGTCGTCTCCGTTATCGCTCCCAACACTCCGGCCATGTACGAACTCCACTTCGCCGTTCCGATGGTTGGTGCCGTCCTTAATACCATCAATCTCCGCCTCGACCCTCGAACCATCTCCGTCCTCCTCCGCCACAGCGAAACCAAACTCATCTTCGTCGATCAAGCCTCTTGTGCTCTAATCTCCGACGCTTTGGCCTTGTTCCCTTCGGAGACGAAACGGCCGTTACTCGTCCTCATTGCCGACGACACTGCGGTACAAGATTCGTCATTGTCATCCTCTGTTTTGATTCGCAACGATTTTGTCGATTCGTACGAGGAAATGGTTTGGAAAGGGGATTCTAATTTCGAATGGATAAGACCGGTGAGTGAATGGGATCCGATGGTTTTGAATTACACTTCTGGAACAACGTCGTCGCCGAAAGGAGTGGTTCATTGTCACAGGGGAATTTTCACGGTGGCACTCGATTCGCTTGTGGAATGGGGAGTTCCTAAGCAATCAGTTTATTTATGGACTTTACCAATGTTTCATGCCAATGGGTGGAGTTTTCCTTGGGGAATTGCTGCCGTAGGTGGAACAAATGTTTGTATTCGAAAATTCGATGCATCATTAATTTTTTCCCTGATTCGTCGCCATCATGTGACACACTTATGTGGAGCTCCGGTCGTACTCAATATGCTAACGAACTCTCCTGACAATCGACCACTTGATCGACCGGTCAAGATTCTGACTGCCGGAGCACCACCGCCTGCGGCAGTTCTGCTTCGAATTGAATCATTGGGGTTTGTTGTCACTCATGGGTATGGATTAACTGAGAGTGGTGGGACGATCATCAGCTGTTCATGGAAGTCGGAGTGGAACCGGCTGACGCAAACAGAGCAAGCACGGCTGAAGGCGAGGCAGGGGGTACGGATGCTAGCGGTTGAGGCGGCGGATGTGTTAGATCCAAAGACTAGAAAAAGTGTGAAAAGAGACGGAGCTTCCATAGGAGAGATTGTTGTGAGAGGCGGATCGATAATGCTTGGGTATTTGAAAGATCCAGAAGCGACAGCGAAAGCGATGACCGACGACGGGTGGTTCTTCACCGGAGATGTGGGGGTGATGCATCCAGATGGGTATATAGAGATAAAGGACAGATCGAAGGATGTGATTATTAGTGGAGGAGAGAATTTGAGCAGCGTTGAGGTGGAATCAGTTCTGTACATGAATCCGGTGGTGAATGAAGTGGCAGTAGTGGCACGACCGGACGAGTTCTGGGGAGAGACACCGTGTGCTTTTGTGAACTTGAAAAAAGGATTGACTCGACAACCGACGGAGGAAGAGATTATTGAGTTTTGTAAAGGGAAGCTGCCACGATTTATGGTGCCTAAGACGGTGGTCTTCACCACGGAGCTTCCAAAGACATCAACCGGAAAAATTCTGAAGTCTGTGCTCAGAGAAATGGCCAAGTCAATGGGCCCCACACCAAAAAGTAGATTGTAA

Protein sequence

MENLKPRPANSSPLTPIGFIERTALIYGDSPSIIYNSTTYTWSETHRRCLQLASSLSSIGIQRGHVVSVIAPNTPAMYELHFAVPMVGAVLNTINLRLDPRTISVLLRHSETKLIFVDQASCALISDALALFPSETKRPLLVLIADDTAVQDSSLSSSVLIRNDFVDSYEEMVWKGDSNFEWIRPVSEWDPMVLNYTSGTTSSPKGVVHCHRGIFTVALDSLVEWGVPKQSVYLWTLPMFHANGWSFPWGIAAVGGTNVCIRKFDASLIFSLIRRHHVTHLCGAPVVLNMLTNSPDNRPLDRPVKILTAGAPPPAAVLLRIESLGFVVTHGYGLTESGGTIISCSWKSEWNRLTQTEQARLKARQGVRMLAVEAADVLDPKTRKSVKRDGASIGEIVVRGGSIMLGYLKDPEATAKAMTDDGWFFTGDVGVMHPDGYIEIKDRSKDVIISGGENLSSVEVESVLYMNPVVNEVAVVARPDEFWGETPCAFVNLKKGLTRQPTEEEIIEFCKGKLPRFMVPKTVVFTTELPKTSTGKILKSVLREMAKSMGPTPKSRL
Homology
BLAST of Cla97C01G008675 vs. NCBI nr
Match: XP_008463174.1 (PREDICTED: probable acyl-activating enzyme 5, peroxisomal [Cucumis melo] >KAA0046220.1 putative acyl-activating enzyme 5, peroxisomal [Cucumis melo var. makuwa] >TYK18976.1 putative acyl-activating enzyme 5, peroxisomal [Cucumis melo var. makuwa])

HSP 1 Score: 997.7 bits (2578), Expect = 4.1e-287
Identity = 486/557 (87.25%), Postives = 521/557 (93.54%), Query Frame = 0

Query: 1   MENLKPRPANSSPLTPIGFIERTALIYGDSPSIIYNSTTYTWSETHRRCLQLASSLSSIG 60
           ME+LKP PANS PLTPIGFI+R A IYGDS SIIYNSTTYTWSETHRRCLQLASS+SS G
Sbjct: 1   MEDLKPTPANSFPLTPIGFIQRAATIYGDSTSIIYNSTTYTWSETHRRCLQLASSISSFG 60

Query: 61  IQRGHVVSVIAPNTPAMYELHFAVPMVGAVLNTINLRLDPRTISVLLRHSETKLIFVDQA 120
           IQRGHVVSVIAPNTPAMYELHFAVPMVGA+LNTINLRLD RTIS+LLRHSE+KLIFVDQA
Sbjct: 61  IQRGHVVSVIAPNTPAMYELHFAVPMVGAILNTINLRLDTRTISILLRHSESKLIFVDQA 120

Query: 121 SCALISDALALFPSETKRPLLVLIADDTAVQDSSLSSSVLIRNDFVDSYEEMVWKGDSNF 180
           SCALI +ALALFP E KRPLLVLIADD A QDSSL S V IR++FVDSYE+MV +GDS F
Sbjct: 121 SCALIFEALALFPPEMKRPLLVLIADDAAEQDSSLPSFVSIRDNFVDSYEDMVKRGDSKF 180

Query: 181 EWIRPVSEWDPMVLNYTSGTTSSPKGVVHCHRGIFTVALDSLVEWGVPKQSVYLWTLPMF 240
           +WIRP+SEWDP+VLNYTSGTTSSPKGVVHCHRGIFTVALDSL+EWGVPKQSVYLWTLPMF
Sbjct: 181 QWIRPLSEWDPIVLNYTSGTTSSPKGVVHCHRGIFTVALDSLLEWGVPKQSVYLWTLPMF 240

Query: 241 HANGWSFPWGIAAVGGTNVCIRKFDASLIFSLIRRHHVTHLCGAPVVLNMLTNSPDNRPL 300
           H NGWSFPWGIAAVGGTNVCIRKFDA LIFSLIRRHHVTHLCGAPVVLNMLTNSPDN PL
Sbjct: 241 HGNGWSFPWGIAAVGGTNVCIRKFDARLIFSLIRRHHVTHLCGAPVVLNMLTNSPDNLPL 300

Query: 301 DRPVKILTAGAPPPAAVLLRIESLGFVVTHGYGLTESGGTIISCSWKSEWNRLTQTEQAR 360
           DRPVKILTAGAPPPA++LLR ESLGFVVTHGYGLTES GTII CSWKSEWN+L   EQAR
Sbjct: 301 DRPVKILTAGAPPPASILLRTESLGFVVTHGYGLTESAGTIICCSWKSEWNQLPAIEQAR 360

Query: 361 LKARQGVRMLAVEAADVLDPKTRKSVKRDGASIGEIVVRGGSIMLGYLKDPEATAKAMTD 420
           LKARQGVRMLAVEA DVLDP+TRKSVKRDGASIGEIVVRG S+ LGYLKDPE TAKAM++
Sbjct: 361 LKARQGVRMLAVEAVDVLDPETRKSVKRDGASIGEIVVRGASVTLGYLKDPEGTAKAMSE 420

Query: 421 DGWFFTGDVGVMHPDGYIEIKDRSKDVIISGGENLSSVEVESVLYMNPVVNEVAVVARPD 480
           DGWFFTGDVGVMHPDGY+EIKDRSKDVIISGGENLSSVEVESVLYMNP+VNE AVVARPD
Sbjct: 421 DGWFFTGDVGVMHPDGYLEIKDRSKDVIISGGENLSSVEVESVLYMNPMVNEAAVVARPD 480

Query: 481 EFWGETPCAFVNLKKGLTRQPTEEEIIEFCKGKLPRFMVPKTVVFTTELPKTSTGKILKS 540
           EFWGETPCAF++LK+GL+R+PTEEEIIE+CKGKLPRFMVPKTV+F TE+PKT+TGKI K 
Sbjct: 481 EFWGETPCAFISLKEGLSRKPTEEEIIEYCKGKLPRFMVPKTVIFMTEMPKTATGKIQKF 540

Query: 541 VLREMAKSMGPTPKSRL 558
           VLR+ AKS+GP PKSRL
Sbjct: 541 VLRQKAKSLGPAPKSRL 557

BLAST of Cla97C01G008675 vs. NCBI nr
Match: XP_004148908.1 (probable acyl-activating enzyme 5, peroxisomal [Cucumis sativus] >KGN50898.1 hypothetical protein Csa_023091 [Cucumis sativus])

HSP 1 Score: 983.8 bits (2542), Expect = 6.1e-283
Identity = 479/557 (86.00%), Postives = 517/557 (92.82%), Query Frame = 0

Query: 1   MENLKPRPANSSPLTPIGFIERTALIYGDSPSIIYNSTTYTWSETHRRCLQLASSLSSIG 60
           ME+LKPRPANS PLTPIGFIER A+IYGDS SIIYNSTTYTWSETHRRCLQLASS+SS G
Sbjct: 1   MEDLKPRPANSFPLTPIGFIERAAIIYGDSTSIIYNSTTYTWSETHRRCLQLASSISSFG 60

Query: 61  IQRGHVVSVIAPNTPAMYELHFAVPMVGAVLNTINLRLDPRTISVLLRHSETKLIFVDQA 120
           IQRGHVVSV APNTPAMYELHFAVPM GA+LNTINLRLD RTIS+LLRHSE+KLIFVDQ 
Sbjct: 61  IQRGHVVSVFAPNTPAMYELHFAVPMAGAILNTINLRLDTRTISILLRHSESKLIFVDQP 120

Query: 121 SCALISDALALFPSETKRPLLVLIADDTAVQDSSLSSSVLIRNDFVDSYEEMVWKGDSNF 180
           SCALI DALALFP + KRPLLVLI DDTA QDSSL S V IR++FVDSYE MV  GDS F
Sbjct: 121 SCALIFDALALFPPDMKRPLLVLITDDTAEQDSSLPSVVSIRDNFVDSYENMVKSGDSKF 180

Query: 181 EWIRPVSEWDPMVLNYTSGTTSSPKGVVHCHRGIFTVALDSLVEWGVPKQSVYLWTLPMF 240
           +WIRP+SEWDP+VLNYTSGTTS+PKGVV+CHRG+FTVALDS++EWGVPKQSVYLWTLPMF
Sbjct: 181 QWIRPLSEWDPIVLNYTSGTTSAPKGVVNCHRGVFTVALDSILEWGVPKQSVYLWTLPMF 240

Query: 241 HANGWSFPWGIAAVGGTNVCIRKFDASLIFSLIRRHHVTHLCGAPVVLNMLTNSPDNRPL 300
           H NGWSFPWGIAAVGGTNVCIRKFD+ LIFSLIRRHHVTHLCGAPVVLNMLTNSPDN PL
Sbjct: 241 HGNGWSFPWGIAAVGGTNVCIRKFDSRLIFSLIRRHHVTHLCGAPVVLNMLTNSPDNLPL 300

Query: 301 DRPVKILTAGAPPPAAVLLRIESLGFVVTHGYGLTESGGTIISCSWKSEWNRLTQTEQAR 360
           DRPVKILTAGAPPPA+VLLR ESLGFVVTHGYGLTES GTII CSWKSEWN+L   EQAR
Sbjct: 301 DRPVKILTAGAPPPASVLLRTESLGFVVTHGYGLTESAGTIICCSWKSEWNQLPAIEQAR 360

Query: 361 LKARQGVRMLAVEAADVLDPKTRKSVKRDGASIGEIVVRGGSIMLGYLKDPEATAKAMTD 420
           LKARQGVRMLAVEA DVLDP+TRKSVKRDGASIGEIVVRG S+MLGYLKD EAT+KAM++
Sbjct: 361 LKARQGVRMLAVEAVDVLDPETRKSVKRDGASIGEIVVRGPSLMLGYLKDTEATSKAMSE 420

Query: 421 DGWFFTGDVGVMHPDGYIEIKDRSKDVIISGGENLSSVEVESVLYMNPVVNEVAVVARPD 480
           DGWFFTGDVGVMHPDGYIEIKDRSKDVIISGGENLSSVEVESVLYMNP+VNE AVVARPD
Sbjct: 421 DGWFFTGDVGVMHPDGYIEIKDRSKDVIISGGENLSSVEVESVLYMNPMVNEAAVVARPD 480

Query: 481 EFWGETPCAFVNLKKGLTRQPTEEEIIEFCKGKLPRFMVPKTVVFTTELPKTSTGKILKS 540
           EFWGETPCAF++LK+GL+R+PTEEEIIE+CKGKLP+FMVPKTV+F TE+PKT+TGK  K 
Sbjct: 481 EFWGETPCAFISLKEGLSRKPTEEEIIEYCKGKLPKFMVPKTVIFMTEMPKTATGKFQKF 540

Query: 541 VLREMAKSMGPTPKSRL 558
           VLR+ AKS+GP  KSRL
Sbjct: 541 VLRQKAKSLGPPQKSRL 557

BLAST of Cla97C01G008675 vs. NCBI nr
Match: XP_022939278.1 (probable acyl-activating enzyme 5, peroxisomal [Cucurbita moschata] >KAG6579057.1 putative acyl-activating enzyme 5, peroxisomal, partial [Cucurbita argyrosperma subsp. sororia])

HSP 1 Score: 961.8 bits (2485), Expect = 2.5e-276
Identity = 471/557 (84.56%), Postives = 505/557 (90.66%), Query Frame = 0

Query: 1   MENLKPRPANSSPLTPIGFIERTALIYGDSPSIIYNSTTYTWSETHRRCLQLASSLSSIG 60
           ME+LKP PANSSPLTPIGF+ER A++YGD PSIIYNSTTYTW+ETHRRCLQLASSL SIG
Sbjct: 1   MEDLKPNPANSSPLTPIGFLERAAVVYGDCPSIIYNSTTYTWTETHRRCLQLASSLVSIG 60

Query: 61  IQRGHVVSVIAPNTPAMYELHFAVPMVGAVLNTINLRLDPRTISVLLRHSETKLIFVDQA 120
           IQRG VVSV++PNT AMYELHFAVPM GAVLNTINLRLD RTISV+LRH ET LIFVDQA
Sbjct: 61  IQRGQVVSVVSPNTAAMYELHFAVPMAGAVLNTINLRLDARTISVILRHCETNLIFVDQA 120

Query: 121 SCALISDALALFPSETKRPLLVLIADDTAVQDSSLSSSVLIRNDFVDSYEEMVWKGDSNF 180
           SC LISDALALFP E KRP L+LIAD+ A +DSS +SSV  R DFVDSYEEMV KGD  F
Sbjct: 121 SCGLISDALALFPPEMKRPPLILIADNAAAEDSSSASSVWNRADFVDSYEEMVQKGDPLF 180

Query: 181 EWIRPVSEWDPMVLNYTSGTTSSPKGVVHCHRGIFTVALDSLVEWGVPKQSVYLWTLPMF 240
           +WIRP SEWDP+VLNYTSGTTSSPKGVVHCHRGIFTV LDSL+EWGVPK+SVYLWTLPMF
Sbjct: 181 QWIRPASEWDPIVLNYTSGTTSSPKGVVHCHRGIFTVTLDSLIEWGVPKESVYLWTLPMF 240

Query: 241 HANGWSFPWGIAAVGGTNVCIRKFDASLIFSLIRRHHVTHLCGAPVVLNMLTNSPDNRPL 300
           HANGWSFPWGIAAVGGTNVCIRKFDA+ IF+LI R+HVTHLCGAPVVLNMLTNSPDNRPL
Sbjct: 241 HANGWSFPWGIAAVGGTNVCIRKFDAAAIFALIHRYHVTHLCGAPVVLNMLTNSPDNRPL 300

Query: 301 DRPVKILTAGAPPPAAVLLRIESLGFVVTHGYGLTESGGTIISCSWKSEWNRLTQTEQAR 360
           DRPVKILTAGAPPPAAVL R ESLGF V+HGYGLTE+GG +I CSWKS WNRL   E+AR
Sbjct: 301 DRPVKILTAGAPPPAAVLFRTESLGFDVSHGYGLTETGGLVICCSWKSGWNRLPAAERAR 360

Query: 361 LKARQGVRMLAVEAADVLDPKTRKSVKRDGASIGEIVVRGGSIMLGYLKDPEATAKAMTD 420
           LKARQGVR LA+  ADVLDP+T KSVKRDG SIGEIV+RG S+MLGYLKDPEATAKAMT 
Sbjct: 361 LKARQGVRTLAMAVADVLDPETGKSVKRDGTSIGEIVLRGSSMMLGYLKDPEATAKAMTA 420

Query: 421 DGWFFTGDVGVMHPDGYIEIKDRSKDVIISGGENLSSVEVESVLYMNPVVNEVAVVARPD 480
           +GWFFTGDVGVMHPDGY+EIKDRSKDVIISGGENLSSVEVESVLY NP VNE AVVARPD
Sbjct: 421 EGWFFTGDVGVMHPDGYLEIKDRSKDVIISGGENLSSVEVESVLYTNPAVNEAAVVARPD 480

Query: 481 EFWGETPCAFVNLKKGLTRQPTEEEIIEFCKGKLPRFMVPKTVVFTTELPKTSTGKILKS 540
           EFWGETPCAFV+LK+GLTR+PTE EIIE+C+GKLPRFMVPKTVVFT ELPKTSTGKI K 
Sbjct: 481 EFWGETPCAFVSLKEGLTRRPTEGEIIEYCRGKLPRFMVPKTVVFTAELPKTSTGKIQKF 540

Query: 541 VLREMAKSMGPTPKSRL 558
           VLREMAKSMGP  KSRL
Sbjct: 541 VLREMAKSMGPAGKSRL 557

BLAST of Cla97C01G008675 vs. NCBI nr
Match: XP_022993747.1 (probable acyl-activating enzyme 5, peroxisomal [Cucurbita maxima])

HSP 1 Score: 953.0 bits (2462), Expect = 1.2e-273
Identity = 471/561 (83.96%), Postives = 504/561 (89.84%), Query Frame = 0

Query: 1   MENLKPRPANSSPLTPIGFIERTALIYGDSPSIIYNSTTYTWSETHRRCLQLASSLSSIG 60
           ME+LKP PANSSPLTPIGF+ER A++YGD PSIIYNSTTYTW+ETHRRCLQLASSL SIG
Sbjct: 1   MEDLKPNPANSSPLTPIGFLERAAVVYGDCPSIIYNSTTYTWTETHRRCLQLASSLVSIG 60

Query: 61  IQRGHVVSVIAPNTPAMYELHFAVPMVGAVLNTINLRLDPRTISVLLRHSETKLIFVDQA 120
           IQRG VVSV++PNTPAMYELHFAVPM GAVLNTINLRLD RTISV+LRH ET LIFVDQA
Sbjct: 61  IQRGQVVSVVSPNTPAMYELHFAVPMAGAVLNTINLRLDSRTISVILRHCETNLIFVDQA 120

Query: 121 SCALISDALALFPSETKRPLLVLIADDTAVQDSSLS----SSVLIRNDFVDSYEEMVWKG 180
           SC LISDALALFP E KRP L+LIAD+ A +DSS S    SSV  R DFVDSYEEMV KG
Sbjct: 121 SCGLISDALALFPPEMKRPPLILIADNAAAEDSSSSSSSASSVSNRADFVDSYEEMVQKG 180

Query: 181 DSNFEWIRPVSEWDPMVLNYTSGTTSSPKGVVHCHRGIFTVALDSLVEWGVPKQSVYLWT 240
           D  F+WIRPVSEWDP+VLNYTSGTTSSPKGVVHCHRGIFTV LDSL+EWGV KQSVYLWT
Sbjct: 181 DPLFQWIRPVSEWDPIVLNYTSGTTSSPKGVVHCHRGIFTVTLDSLIEWGVSKQSVYLWT 240

Query: 241 LPMFHANGWSFPWGIAAVGGTNVCIRKFDASLIFSLIRRHHVTHLCGAPVVLNMLTNSPD 300
           LPMFHANGWSFPWGIAAVGGTNVCIRKFDA+ IF+LI R+HVTHLCGAPVVLNMLTNSPD
Sbjct: 241 LPMFHANGWSFPWGIAAVGGTNVCIRKFDAATIFALIHRYHVTHLCGAPVVLNMLTNSPD 300

Query: 301 NRPLDRPVKILTAGAPPPAAVLLRIESLGFVVTHGYGLTESGGTIISCSWKSEWNRLTQT 360
           NRPLDRPVKILTAGAPPPAAVL R ESLGF V+HGYGLTE+GG +I CSWKS WNRL   
Sbjct: 301 NRPLDRPVKILTAGAPPPAAVLFRTESLGFDVSHGYGLTETGGLVICCSWKSGWNRLPAA 360

Query: 361 EQARLKARQGVRMLAVEAADVLDPKTRKSVKRDGASIGEIVVRGGSIMLGYLKDPEATAK 420
           E+ARLKARQGVR LA+  ADVL P+T KSVKRDG SIGEIV+RG S+MLGYLKDPEATAK
Sbjct: 361 ERARLKARQGVRTLAMAVADVLCPETGKSVKRDGTSIGEIVLRGSSMMLGYLKDPEATAK 420

Query: 421 AMTDDGWFFTGDVGVMHPDGYIEIKDRSKDVIISGGENLSSVEVESVLYMNPVVNEVAVV 480
           AMT +GWFFTGDVGVMHPDGY+EIKDRSKDVIISGGENLSSVEVESVLY NP VNE AVV
Sbjct: 421 AMTAEGWFFTGDVGVMHPDGYLEIKDRSKDVIISGGENLSSVEVESVLYTNPAVNEAAVV 480

Query: 481 ARPDEFWGETPCAFVNLKKGLTRQPTEEEIIEFCKGKLPRFMVPKTVVFTTELPKTSTGK 540
           ARPDEFWGETPCAFV+LK+GLTR+PTE EIIE+C+GKLPRFMVPKTVVF  ELPKTSTGK
Sbjct: 481 ARPDEFWGETPCAFVSLKEGLTRRPTEGEIIEYCRGKLPRFMVPKTVVFMAELPKTSTGK 540

Query: 541 ILKSVLREMAKSMGPTPKSRL 558
           I K VLREMAKSMGP  +SRL
Sbjct: 541 IQKFVLREMAKSMGPAGRSRL 561

BLAST of Cla97C01G008675 vs. NCBI nr
Match: XP_023550909.1 (probable acyl-activating enzyme 5, peroxisomal [Cucurbita pepo subsp. pepo])

HSP 1 Score: 953.0 bits (2462), Expect = 1.2e-273
Identity = 470/561 (83.78%), Postives = 505/561 (90.02%), Query Frame = 0

Query: 1   MENLKPRPANSSPLTPIGFIERTALIYGDSPSIIYNSTTYTWSETHRRCLQLASSLSSIG 60
           ME+LKP PANSSPLTPIGF+ER A++YGD  SIIY+STTYTW+ETHRRCLQLASSL SIG
Sbjct: 1   MEDLKPNPANSSPLTPIGFLERAAVVYGDCRSIIYDSTTYTWTETHRRCLQLASSLVSIG 60

Query: 61  IQRGHVVSVIAPNTPAMYELHFAVPMVGAVLNTINLRLDPRTISVLLRHSETKLIFVDQA 120
           IQRG VVSV++PNT AMYELHFAVPM GAVLNTINLRLD RTISV+LRH ET LIFVDQA
Sbjct: 61  IQRGQVVSVVSPNTAAMYELHFAVPMAGAVLNTINLRLDARTISVILRHCETNLIFVDQA 120

Query: 121 SCALISDALALFPSETKRPLLVLIADDTAVQDSSLS----SSVLIRNDFVDSYEEMVWKG 180
           SC LISDALALFP E KRP L+LIAD+ A +DSS S    SSVL R DFVDSYEEMV KG
Sbjct: 121 SCGLISDALALFPPEMKRPPLILIADNAAAEDSSSSSSSASSVLNRADFVDSYEEMVQKG 180

Query: 181 DSNFEWIRPVSEWDPMVLNYTSGTTSSPKGVVHCHRGIFTVALDSLVEWGVPKQSVYLWT 240
           D  F+WIRP SEWDP+VLNYTSGTTSSPKGVVHCHRGIFTV LDSL+EWGVPK+SVYLWT
Sbjct: 181 DPLFQWIRPASEWDPIVLNYTSGTTSSPKGVVHCHRGIFTVTLDSLIEWGVPKESVYLWT 240

Query: 241 LPMFHANGWSFPWGIAAVGGTNVCIRKFDASLIFSLIRRHHVTHLCGAPVVLNMLTNSPD 300
           LPMFHANGWSFPWGIAAVGGTNVCIRKFDA+ IF+LI R+HVTHLCGAPVVLNMLTNSPD
Sbjct: 241 LPMFHANGWSFPWGIAAVGGTNVCIRKFDAAAIFALIHRYHVTHLCGAPVVLNMLTNSPD 300

Query: 301 NRPLDRPVKILTAGAPPPAAVLLRIESLGFVVTHGYGLTESGGTIISCSWKSEWNRLTQT 360
           NRPLDRPVKILTAGAPPPAAVL R ESLGF V+HGYGLTE+GG +I CSWKS WNRL   
Sbjct: 301 NRPLDRPVKILTAGAPPPAAVLFRTESLGFDVSHGYGLTETGGLVICCSWKSGWNRLPAA 360

Query: 361 EQARLKARQGVRMLAVEAADVLDPKTRKSVKRDGASIGEIVVRGGSIMLGYLKDPEATAK 420
           ++ARLKARQGVR LA+  ADVLDP+T KSVKRDG SIGEIV+RG S+MLGYLKDPEATAK
Sbjct: 361 DRARLKARQGVRTLAMAVADVLDPETGKSVKRDGTSIGEIVLRGSSMMLGYLKDPEATAK 420

Query: 421 AMTDDGWFFTGDVGVMHPDGYIEIKDRSKDVIISGGENLSSVEVESVLYMNPVVNEVAVV 480
           AMT +GWFFTGDVGVMHPDGY+EIKDRSKDVIISGGENLSSVEVESVLY NP VNE AVV
Sbjct: 421 AMTAEGWFFTGDVGVMHPDGYLEIKDRSKDVIISGGENLSSVEVESVLYTNPAVNEAAVV 480

Query: 481 ARPDEFWGETPCAFVNLKKGLTRQPTEEEIIEFCKGKLPRFMVPKTVVFTTELPKTSTGK 540
           ARPDEFWGETPCAFV+LK+GLTR+PTE EIIE+C+GKLPRFMVPKTVVFT ELPKTSTGK
Sbjct: 481 ARPDEFWGETPCAFVSLKEGLTRRPTEGEIIEYCRGKLPRFMVPKTVVFTAELPKTSTGK 540

Query: 541 ILKSVLREMAKSMGPTPKSRL 558
           I K VLREMAKSMGP  KSRL
Sbjct: 541 IQKFVLREMAKSMGPAGKSRL 561

BLAST of Cla97C01G008675 vs. ExPASy Swiss-Prot
Match: M4IQQ5 (2-methylpropanoate--CoA ligase CCL4 OS=Humulus lupulus OX=3486 GN=CCL4 PE=1 SV=1)

HSP 1 Score: 815.1 bits (2104), Expect = 5.0e-235
Identity = 375/550 (68.18%), Postives = 466/550 (84.73%), Query Frame = 0

Query: 1   MENLKPRPANSSPLTPIGFIERTALIYGDSPSIIYNSTTYTWSETHRRCLQLASSLSSIG 60
           ME+LKPRPA+SSPLTP+GF+ER A +YGD  S++Y++ +YTWS+THRRCL LASS++S+G
Sbjct: 1   MEDLKPRPASSSPLTPLGFLERAATVYGDCTSVVYDAVSYTWSQTHRRCLCLASSIASLG 60

Query: 61  IQRGHVVSVIAPNTPAMYELHFAVPMVGAVLNTINLRLDPRTISVLLRHSETKLIFVDQA 120
           I+ GHVVSV+APN P MYELHFAVPM GA+LN +NLRLD RTIS+LL HSE+KLIFVD  
Sbjct: 61  IENGHVVSVLAPNVPQMYELHFAVPMAGAILNAVNLRLDARTISILLHHSESKLIFVDHL 120

Query: 121 SCALISDALALFPSETKRPLLVLIADDTAVQDSSLSSSVLIRNDFVDSYEEMVWKGDSNF 180
           S  LI +A+ALFP +   P LV +AD     +S   +S  +  +F  SY++++ +GD +F
Sbjct: 121 SRDLILEAIALFPKQAPVPRLVFMAD-----ESESGNSSELGKEFFCSYKDLIDRGDPDF 180

Query: 181 EWIRPVSEWDPMVLNYTSGTTSSPKGVVHCHRGIFTVALDSLVEWGVPKQSVYLWTLPMF 240
           +W+ P SEWDPM+LNYTSGTTSSPKGVVHCHRGIF + +DSL++WGVPKQ VYLWTLPMF
Sbjct: 181 KWVMPKSEWDPMILNYTSGTTSSPKGVVHCHRGIFIMTVDSLIDWGVPKQPVYLWTLPMF 240

Query: 241 HANGWSFPWGIAAVGGTNVCIRKFDASLIFSLIRRHHVTHLCGAPVVLNMLTNSPDNRPL 300
           HANGWS+PWG+AAVGGTN+C+RKFD+ +I+ +I+RH VTH+CGAPVVLNML+N+P + PL
Sbjct: 241 HANGWSYPWGMAAVGGTNICLRKFDSEIIYDMIKRHGVTHMCGAPVVLNMLSNAPGSEPL 300

Query: 301 DRPVKILTAGAPPPAAVLLRIESLGFVVTHGYGLTESGGTIISCSWKSEWNRLTQTEQAR 360
              V+I+TAGAPPP+AVL R ESLGF V+HGYGLTE+ G ++SC+WK EWN L  TE+AR
Sbjct: 301 KTTVQIMTAGAPPPSAVLFRTESLGFAVSHGYGLTETAGLVVSCAWKKEWNHLPATERAR 360

Query: 361 LKARQGVRMLAVEAADVLDPKTRKSVKRDGASIGEIVVRGGSIMLGYLKDPEATAKAMTD 420
           LK+RQGV  +     DV+DP T  +VKRDG+++GE+V+RGGS+MLGYLKDPE TAK+MT 
Sbjct: 361 LKSRQGVGTVMQTKIDVVDPVTGAAVKRDGSTLGEVVLRGGSVMLGYLKDPEGTAKSMTA 420

Query: 421 DGWFFTGDVGVMHPDGYIEIKDRSKDVIISGGENLSSVEVESVLYMNPVVNEVAVVARPD 480
           DGWF+TGDVGVMHPDGY+EIKDRSKDVIISGGENLSSVEVES+LY +P + E AVVARPD
Sbjct: 421 DGWFYTGDVGVMHPDGYLEIKDRSKDVIISGGENLSSVEVESILYSHPDILEAAVVARPD 480

Query: 481 EFWGETPCAFVNLKKGLTRQPTEEEIIEFCKGKLPRFMVPKTVVFTTELPKTSTGKILKS 540
           EFWGETPCAFV+LKKGLT++PTE+EI+E+C+ KLPR+MVPKTVVF  ELPKTSTGK+ K 
Sbjct: 481 EFWGETPCAFVSLKKGLTKKPTEKEIVEYCRSKLPRYMVPKTVVFKEELPKTSTGKVQKF 540

Query: 541 VLREMAKSMG 551
           +LR+MA+ MG
Sbjct: 541 ILRDMARGMG 545

BLAST of Cla97C01G008675 vs. ExPASy Swiss-Prot
Match: M4ISH2 (Probable CoA ligase CCL11 OS=Humulus lupulus OX=3486 GN=CCL11 PE=2 SV=1)

HSP 1 Score: 763.8 bits (1971), Expect = 1.3e-219
Identity = 364/557 (65.35%), Postives = 448/557 (80.43%), Query Frame = 0

Query: 1   MENLKPRPANSSPLTPIGFIERTALIYGDSPSIIYNSTTYTWSETHRRCLQLASSLSS-I 60
           ME LKPR ANS PLTP+GF+ER A +YGD  S++Y+  +YTWS+TH RCL++AS + S +
Sbjct: 1   MEELKPRFANSCPLTPLGFLERAATVYGDCISVVYDDLSYTWSQTHTRCLRVASCIESCL 60

Query: 61  GIQRGHVVSVIAPNTPAMYELHFAVPMVGAVLNTINLRLDPRTISVLLRHSETKLIFVDQ 120
           G+++G VVSV+APN PAMYEL+FAVPM G VLN IN RL+  TISV+LRHSE+KL+FVDQ
Sbjct: 61  GVKKGQVVSVVAPNVPAMYELNFAVPMAGVVLNNINTRLNAVTISVMLRHSESKLVFVDQ 120

Query: 121 ASCALISDALALFPSETKRPLLVLIADDTAVQDSSLSSSVLIRNDFVDSYEEMVWKG-DS 180
            S  L+ DA++LFP  T  PLLVLIAD+   ++ ++      R  FV  YE++V KG D 
Sbjct: 121 LSVRLVLDAVSLFPKNTPTPLLVLIADNITGENLTVED----REHFVCCYEDLVEKGDDK 180

Query: 181 NFEWIRPVSEWDPMVLNYTSGTTSSPKGVVHCHRGIFTVALDSLVEWGVPKQSVYLWTLP 240
            F+W+RP+SEWDP+VLNYTSGTTSSPKGVVH HR +F V LDSL++WGVPKQ VYLWTLP
Sbjct: 181 TFKWVRPISEWDPIVLNYTSGTTSSPKGVVHSHRSVFVVTLDSLIDWGVPKQPVYLWTLP 240

Query: 241 MFHANGWSFPWGIAAVGGTNVCIRKFDASLIFSLIRRHHVTHLCGAPVVLNMLTNSPDNR 300
           MFHANGW + WGIAAVGGTN+C+R+FD  +IF+LIRRH VTH+C AP+VLNML+NSP+  
Sbjct: 241 MFHANGWGYTWGIAAVGGTNICLRRFDGEIIFNLIRRHRVTHMCAAPIVLNMLSNSPNAE 300

Query: 301 PLDRPVKILTAGAPPPAAVLLRIESLGFVVTHGYGLTESGGTIISCSWKSEWNRLTQTEQ 360
           PL  PV ++T GAPPPAAVLLR ESLGFV++HGYG+TE  G ++SC+WK EWNRL  TEQ
Sbjct: 301 PLPNPVHVMTGGAPPPAAVLLRTESLGFVISHGYGMTEMLGVVVSCAWKREWNRLPATEQ 360

Query: 361 ARLKARQGVRMLAVEAADVLDPKTRKSVKRDGASIGEIVVRGGSIMLGYLKDPEATAKAM 420
           ARLK+RQGVR  A+   DV+DP +  SVKRDG ++GEIV++G SIMLGYLK+  ATAK +
Sbjct: 361 ARLKSRQGVRTAAMMEVDVVDPNSGVSVKRDGLTMGEIVLKGSSIMLGYLKNSAATAKCI 420

Query: 421 TDDGWFFTGDVGVMHPDGYIEIKDRSKDVIISGGENLSSVEVESVLYMNPVVNEVAVVAR 480
             DGWF+TGD+ VMHPDGY+EIKDRSKDVIISGGEN+SSVEVES LY +P V+E AVVA 
Sbjct: 421 RADGWFYTGDMAVMHPDGYLEIKDRSKDVIISGGENVSSVEVESALYSHPAVDEAAVVAC 480

Query: 481 PDEFWGETPCAFVNLKKGLTRQPTEEEIIEFCKGKLPRFMVPKTVVFTTELPKTSTGKIL 540
           PDE+WGETP AFV LKKG+  +PTE+EI+E+C+ KL  FMVPK VVF  +LPKTSTGKI 
Sbjct: 481 PDEYWGETPFAFVTLKKGMRVRPTEKEILEYCREKLAHFMVPKVVVFRDKLPKTSTGKIQ 540

Query: 541 KSVLREMAKSMGPTPKS 556
           K  L+E+ K+MG +  S
Sbjct: 541 KFTLKEIVKTMGYSSSS 553

BLAST of Cla97C01G008675 vs. ExPASy Swiss-Prot
Match: Q9FFE6 (Probable acyl-activating enzyme 5, peroxisomal OS=Arabidopsis thaliana OX=3702 GN=AAE5 PE=1 SV=1)

HSP 1 Score: 759.6 bits (1960), Expect = 2.5e-218
Identity = 368/558 (65.95%), Postives = 456/558 (81.72%), Query Frame = 0

Query: 1   MENLKPRPANSSPLTPIGFIERTALIYGDSPSIIYNS-TTYTWSETHRRCLQLASSLSSI 60
           ME +KP  ANS PLTPIGF+ER A +YGD  SI+Y S T YTW ET+ RCL++ASSLSSI
Sbjct: 1   MEQMKPCAANSPPLTPIGFLERAATVYGDCTSIVYGSNTVYTWRETNLRCLRVASSLSSI 60

Query: 61  GIQRGHVVSVIAPNTPAMYELHFAVPMVGAVLNTINLRLDPRTISVLLRHSETKLIFVDQ 120
           GI R  VVSV++PNTPAMYEL FAVPM GA+LN IN RLD RT+SVLLRH  +KL+FVD 
Sbjct: 61  GIGRSDVVSVLSPNTPAMYELQFAVPMSGAILNNINTRLDARTVSVLLRHCGSKLLFVDV 120

Query: 121 ASCALISDALALFPSETKRPLLVLIADDTAVQDSSLSSSVLIRNDFVDSYEEMVWKGDSN 180
            S  L  +A+++    T  P+LV IAD    ++    + V  R  F  +Y++++ +GD +
Sbjct: 121 FSVDLAVEAISMM--TTDPPILVFIADK---EEEGGDADVADRTKFSYTYDDLIHRGDLD 180

Query: 181 FEWIRPVSEWDPMVLNYTSGTTSSPKGVVHCHRGIFTVALDSLVEWGVPKQSVYLWTLPM 240
           F+WIRP SEWDP+VLNYTSGTTS+PKGVVHCHRGIF +++DSL++W VPK  VYLWTLP+
Sbjct: 181 FKWIRPESEWDPVVLNYTSGTTSAPKGVVHCHRGIFVMSIDSLIDWTVPKNPVYLWTLPI 240

Query: 241 FHANGWSFPWGIAAVGGTNVCIRKFDASLIFSLIRRHHVTHLCGAPVVLNMLTNSPDNRP 300
           FHANGWS+PWGIAAVGGTNVC+RKFDA LI+ LIR H VTH+CGAPVVLNML+ + + +P
Sbjct: 241 FHANGWSYPWGIAAVGGTNVCLRKFDAPLIYRLIRDHGVTHMCGAPVVLNMLSATNEFQP 300

Query: 301 LDRPVKILTAGAPPPAAVLLRIESLGFVVTHGYGLTESGGTIISCSWKSEWNRLTQTEQA 360
           L+RPV ILTAGAPPPAAVLLR ES+GFV++HGYGLTE+ G  +SC+WK +WNRL  +++A
Sbjct: 301 LNRPVNILTAGAPPPAAVLLRAESIGFVISHGYGLTETAGLNVSCAWKPQWNRLPASDRA 360

Query: 361 RLKARQGVRMLAVEAADVLDPKTRKSVKRDGASIGEIVVRGGSIMLGYLKDPEATAKAMT 420
           RLKARQGVR +     DV+DP++ +SV+R+G ++GEIV+RG SIMLGYLKDP  T KA+ 
Sbjct: 361 RLKARQGVRTVGFTEIDVVDPESGRSVERNGETVGEIVMRGSSIMLGYLKDPVGTEKAL- 420

Query: 421 DDGWFFTGDVGVMHPDGYIEIKDRSKDVIISGGENLSSVEVESVLYMNPVVNEVAVVARP 480
            +GWF+TGDVGV+H DGY+EIKDRSKD+II+GGEN+SSVEVE+VLY NP VNEVAVVARP
Sbjct: 421 KNGWFYTGDVGVIHSDGYLEIKDRSKDIIITGGENVSSVEVETVLYTNPAVNEVAVVARP 480

Query: 481 DEFWGETPCAFVNLKKGLTRQPTEEEIIEFCKGKLPRFMVPKTVVFTTELPKTSTGKILK 540
           D FWGETPCAFV+LK GLT++PTE E+IE+C+ K+P++MVPKTV F  ELPKTSTGK++K
Sbjct: 481 DVFWGETPCAFVSLKSGLTQRPTEVEMIEYCRKKMPKYMVPKTVSFVDELPKTSTGKVMK 540

Query: 541 SVLREMAKSMGPTPKSRL 558
            VLRE+AK MG T  SR+
Sbjct: 541 FVLREIAKKMGTTRLSRM 552

BLAST of Cla97C01G008675 vs. ExPASy Swiss-Prot
Match: Q9FFE9 (Probable acyl-activating enzyme 6 OS=Arabidopsis thaliana OX=3702 GN=AAE6 PE=2 SV=1)

HSP 1 Score: 740.0 bits (1909), Expect = 2.0e-212
Identity = 355/551 (64.43%), Postives = 448/551 (81.31%), Query Frame = 0

Query: 1   MENLKPRPANSSPLTPIGFIERTALIYGDSPSIIYNS-TTYTWSETHRRCLQLASSLSSI 60
           ME +KP  ANS PLTPIGF+ER A +YGD  SI+Y S T YTW ET+ RCL++ASSLSSI
Sbjct: 1   MEEMKPCAANSPPLTPIGFLERAATVYGDCTSIVYGSNTVYTWRETNLRCLRVASSLSSI 60

Query: 61  GIQRGHVVSVIAPNTPAMYELHFAVPMVGAVLNTINLRLDPRTISVLLRHSETKLIFVDQ 120
           GI R  VVSV++PNTPAMYEL FAVPM GA+LN IN RLD RT+SVLLRH E+KL+FVD 
Sbjct: 61  GIGRSDVVSVLSPNTPAMYELQFAVPMSGAILNNINTRLDARTVSVLLRHCESKLLFVDV 120

Query: 121 ASCALISDALALFPSETKRPLLVLIADDTAVQDSSLSSSVLIRNDFVDSYEEMVWKGDSN 180
            S  L  +A+++    T  P+LV+IAD    ++    + V   + F  +Y++++ +GD  
Sbjct: 121 FSVDLAVEAVSMM--TTDPPILVVIADK---EEEGGVADVADLSKFSYTYDDLIERGDPG 180

Query: 181 FEWIRPVSEWDPMVLNYTSGTTSSPKGVVHCHRGIFTVALDSLVEWGVPKQSVYLWTLPM 240
           F+WIRP SEWDP+VLNYTSGTTS+PKGVVHCHRGIF +++DSL++W VPK  VYLWTLP+
Sbjct: 181 FKWIRPESEWDPVVLNYTSGTTSAPKGVVHCHRGIFVMSVDSLIDWAVPKNPVYLWTLPI 240

Query: 241 FHANGWSFPWGIAAVGGTNVCIRKFDASLIFSLIRRHHVTHLCGAPVVLNMLTNSPDNRP 300
           FH+NGW+ PWGIAAVGGTNVC+RKFDA LI+ LIR H VTH+CGAPVVLNML+ + +++P
Sbjct: 241 FHSNGWTNPWGIAAVGGTNVCLRKFDAPLIYRLIRDHGVTHMCGAPVVLNMLSATQESQP 300

Query: 301 LDRPVKILTAGAPPPAAVLLRIESLGFVVTHGYGLTESGGTIISCSWKSEWNRLTQTEQA 360
           L+ PV ILTAG+PPPA VLLR ES+GFV++HGYGLTE+ G I+SC+WK +WN L  +++A
Sbjct: 301 LNHPVNILTAGSPPPATVLLRAESIGFVISHGYGLTETAGVIVSCAWKPKWNHLPASDRA 360

Query: 361 RLKARQGVRMLAVEAADVLDPKTRKSVKRDGASIGEIVVRGGSIMLGYLKDPEATAKAMT 420
           RLKARQGVR +     DV+DP++  SV+R+G ++GEIV+RG S+MLGYLKDP  T KA+ 
Sbjct: 361 RLKARQGVRTVGFTEIDVVDPESGLSVERNGETVGEIVMRGSSVMLGYLKDPVGTEKAL- 420

Query: 421 DDGWFFTGDVGVMHPDGYIEIKDRSKDVIISGGENLSSVEVESVLYMNPVVNEVAVVARP 480
            +GWF+TGDVGV+H DGY+EIKDRSKD+II+GGEN+SSVEVE+VLY  P VNEVAVVARP
Sbjct: 421 KNGWFYTGDVGVIHSDGYLEIKDRSKDIIITGGENVSSVEVETVLYTIPAVNEVAVVARP 480

Query: 481 DEFWGETPCAFVNLKKGLTRQPTEEEIIEFCKGKLPRFMVPKTVVFTTELPKTSTGKILK 540
           DEFWGETPCAFV+LK G + +PTEEE++E+C+ K+P++MVPKTV F  ELPK+STGK+ K
Sbjct: 481 DEFWGETPCAFVSLKNGFSGKPTEEELMEYCRKKMPKYMVPKTVSFMDELPKSSTGKVTK 540

Query: 541 SVLREMAKSMG 551
            VLR++AK MG
Sbjct: 541 FVLRDIAKKMG 545

BLAST of Cla97C01G008675 vs. ExPASy Swiss-Prot
Match: Q9LQS1 (Probable acyl-activating enzyme 8 OS=Arabidopsis thaliana OX=3702 GN=AAE8 PE=2 SV=1)

HSP 1 Score: 714.9 bits (1844), Expect = 7.0e-205
Identity = 350/551 (63.52%), Postives = 437/551 (79.31%), Query Frame = 0

Query: 1   MENLKPRPANSSPLTPIGFIERTALIYGDSPSIIY-NSTTYTWSETHRRCLQLASSLSSI 60
           ME+LKP  ANS PLT +GF+ER A +YGD  SI+Y NST YTW ET+ RCL +AS+LSSI
Sbjct: 1   MEDLKPSAANSLPLTLLGFLERAATVYGDCTSIVYGNSTVYTWRETNHRCLCVASALSSI 60

Query: 61  GIQRGHVVSVIAPNTPAMYELHFAVPMVGAVLNTINLRLDPRTISVLLRHSETKLIFVDQ 120
           GI R  VVSV++ NTP MYEL F+VPM GA+LN IN RLD RT+SVLLRH E+KL+FVD 
Sbjct: 61  GIGRSDVVSVLSANTPEMYELQFSVPMSGAILNNINTRLDARTVSVLLRHCESKLLFVDF 120

Query: 121 ASCALISDALALFPSETKRPLLVLIADDTAVQDSSLSSSVLIRNDFVDSYEEMVWKGDSN 180
               L  +A+ +       P+LVLIA++   ++    + V  R+ F   Y +++ +G+ +
Sbjct: 121 FYSDLAVEAITML---LNPPILVLIANE---EEEEGGAEVTERSKFCYLYSDLITRGNPD 180

Query: 181 FEWIRPVSEWDPMVLNYTSGTTSSPKGVVHCHRGIFTVALDSLVEWGVPKQSVYLWTLPM 240
           F+WIRP SEWDP+V+NYTSGTTSSPKGVVHCHRGIF + LDSL +W VPK  VYLWTLP+
Sbjct: 181 FKWIRPGSEWDPIVVNYTSGTTSSPKGVVHCHRGIFVMTLDSLTDWAVPKTPVYLWTLPI 240

Query: 241 FHANGWSFPWGIAAVGGTNVCIRKFDASLIFSLIRRHHVTHLCGAPVVLNMLTNSPD-NR 300
           FHANGW++PWGIAAVGGTNVC+RK  A  I+ LIR H VTH+ GAP+VL +L+ S + ++
Sbjct: 241 FHANGWTYPWGIAAVGGTNVCVRKLHAPSIYHLIRDHGVTHMYGAPIVLQILSASQESDQ 300

Query: 301 PLDRPVKILTAGAPPPAAVLLRIESLGFVVTHGYGLTESGGTIISCSWKSEWNRLTQTEQ 360
           PL  PV  LTAG+ PPA VLLR ESLGF+V+HGYGLTE+ G I+SC+WK  WNRL  ++Q
Sbjct: 301 PLKSPVNFLTAGSSPPATVLLRAESLGFIVSHGYGLTETAGVIVSCAWKPNWNRLPASDQ 360

Query: 361 ARLKARQGVRMLAVEAADVLDPKTRKSVKRDGASIGEIVVRGGSIMLGYLKDPEATAKAM 420
           A+LK+RQGVR +     DV+DP++ +SV+RDG ++GEIV+RG SIMLGYLK+P  T  + 
Sbjct: 361 AQLKSRQGVRTVGFSEIDVVDPESGRSVERDGETVGEIVLRGSSIMLGYLKNPIGTQNSF 420

Query: 421 TDDGWFFTGDVGVMHPDGYIEIKDRSKDVIISGGENLSSVEVESVLYMNPVVNEVAVVAR 480
             +GWFFTGD+GV+H DGY+EIKDRSKDVIISGGEN+SSVEVE+VLY NP VNE AVVAR
Sbjct: 421 -KNGWFFTGDLGVIHGDGYLEIKDRSKDVIISGGENVSSVEVEAVLYTNPAVNEAAVVAR 480

Query: 481 PDEFWGETPCAFVNLKKGLTRQPTEEEIIEFCKGKLPRFMVPKTVVFTTELPKTSTGKIL 540
           PDEFWGETPCAFV+LK GLTR+PT++EIIE+CK K+PR+M PKTV F  ELPKTSTGKI+
Sbjct: 481 PDEFWGETPCAFVSLKPGLTRKPTDKEIIEYCKYKMPRYMAPKTVSFLEELPKTSTGKII 540

Query: 541 KSVLREMAKSM 550
           KS+L+E+AK+M
Sbjct: 541 KSLLKEIAKNM 544

BLAST of Cla97C01G008675 vs. ExPASy TrEMBL
Match: A0A5D3D623 (Putative acyl-activating enzyme 5, peroxisomal OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold418G00690 PE=4 SV=1)

HSP 1 Score: 997.7 bits (2578), Expect = 2.0e-287
Identity = 486/557 (87.25%), Postives = 521/557 (93.54%), Query Frame = 0

Query: 1   MENLKPRPANSSPLTPIGFIERTALIYGDSPSIIYNSTTYTWSETHRRCLQLASSLSSIG 60
           ME+LKP PANS PLTPIGFI+R A IYGDS SIIYNSTTYTWSETHRRCLQLASS+SS G
Sbjct: 1   MEDLKPTPANSFPLTPIGFIQRAATIYGDSTSIIYNSTTYTWSETHRRCLQLASSISSFG 60

Query: 61  IQRGHVVSVIAPNTPAMYELHFAVPMVGAVLNTINLRLDPRTISVLLRHSETKLIFVDQA 120
           IQRGHVVSVIAPNTPAMYELHFAVPMVGA+LNTINLRLD RTIS+LLRHSE+KLIFVDQA
Sbjct: 61  IQRGHVVSVIAPNTPAMYELHFAVPMVGAILNTINLRLDTRTISILLRHSESKLIFVDQA 120

Query: 121 SCALISDALALFPSETKRPLLVLIADDTAVQDSSLSSSVLIRNDFVDSYEEMVWKGDSNF 180
           SCALI +ALALFP E KRPLLVLIADD A QDSSL S V IR++FVDSYE+MV +GDS F
Sbjct: 121 SCALIFEALALFPPEMKRPLLVLIADDAAEQDSSLPSFVSIRDNFVDSYEDMVKRGDSKF 180

Query: 181 EWIRPVSEWDPMVLNYTSGTTSSPKGVVHCHRGIFTVALDSLVEWGVPKQSVYLWTLPMF 240
           +WIRP+SEWDP+VLNYTSGTTSSPKGVVHCHRGIFTVALDSL+EWGVPKQSVYLWTLPMF
Sbjct: 181 QWIRPLSEWDPIVLNYTSGTTSSPKGVVHCHRGIFTVALDSLLEWGVPKQSVYLWTLPMF 240

Query: 241 HANGWSFPWGIAAVGGTNVCIRKFDASLIFSLIRRHHVTHLCGAPVVLNMLTNSPDNRPL 300
           H NGWSFPWGIAAVGGTNVCIRKFDA LIFSLIRRHHVTHLCGAPVVLNMLTNSPDN PL
Sbjct: 241 HGNGWSFPWGIAAVGGTNVCIRKFDARLIFSLIRRHHVTHLCGAPVVLNMLTNSPDNLPL 300

Query: 301 DRPVKILTAGAPPPAAVLLRIESLGFVVTHGYGLTESGGTIISCSWKSEWNRLTQTEQAR 360
           DRPVKILTAGAPPPA++LLR ESLGFVVTHGYGLTES GTII CSWKSEWN+L   EQAR
Sbjct: 301 DRPVKILTAGAPPPASILLRTESLGFVVTHGYGLTESAGTIICCSWKSEWNQLPAIEQAR 360

Query: 361 LKARQGVRMLAVEAADVLDPKTRKSVKRDGASIGEIVVRGGSIMLGYLKDPEATAKAMTD 420
           LKARQGVRMLAVEA DVLDP+TRKSVKRDGASIGEIVVRG S+ LGYLKDPE TAKAM++
Sbjct: 361 LKARQGVRMLAVEAVDVLDPETRKSVKRDGASIGEIVVRGASVTLGYLKDPEGTAKAMSE 420

Query: 421 DGWFFTGDVGVMHPDGYIEIKDRSKDVIISGGENLSSVEVESVLYMNPVVNEVAVVARPD 480
           DGWFFTGDVGVMHPDGY+EIKDRSKDVIISGGENLSSVEVESVLYMNP+VNE AVVARPD
Sbjct: 421 DGWFFTGDVGVMHPDGYLEIKDRSKDVIISGGENLSSVEVESVLYMNPMVNEAAVVARPD 480

Query: 481 EFWGETPCAFVNLKKGLTRQPTEEEIIEFCKGKLPRFMVPKTVVFTTELPKTSTGKILKS 540
           EFWGETPCAF++LK+GL+R+PTEEEIIE+CKGKLPRFMVPKTV+F TE+PKT+TGKI K 
Sbjct: 481 EFWGETPCAFISLKEGLSRKPTEEEIIEYCKGKLPRFMVPKTVIFMTEMPKTATGKIQKF 540

Query: 541 VLREMAKSMGPTPKSRL 558
           VLR+ AKS+GP PKSRL
Sbjct: 541 VLRQKAKSLGPAPKSRL 557

BLAST of Cla97C01G008675 vs. ExPASy TrEMBL
Match: A0A1S3CIL0 (probable acyl-activating enzyme 5, peroxisomal OS=Cucumis melo OX=3656 GN=LOC103501381 PE=4 SV=1)

HSP 1 Score: 997.7 bits (2578), Expect = 2.0e-287
Identity = 486/557 (87.25%), Postives = 521/557 (93.54%), Query Frame = 0

Query: 1   MENLKPRPANSSPLTPIGFIERTALIYGDSPSIIYNSTTYTWSETHRRCLQLASSLSSIG 60
           ME+LKP PANS PLTPIGFI+R A IYGDS SIIYNSTTYTWSETHRRCLQLASS+SS G
Sbjct: 1   MEDLKPTPANSFPLTPIGFIQRAATIYGDSTSIIYNSTTYTWSETHRRCLQLASSISSFG 60

Query: 61  IQRGHVVSVIAPNTPAMYELHFAVPMVGAVLNTINLRLDPRTISVLLRHSETKLIFVDQA 120
           IQRGHVVSVIAPNTPAMYELHFAVPMVGA+LNTINLRLD RTIS+LLRHSE+KLIFVDQA
Sbjct: 61  IQRGHVVSVIAPNTPAMYELHFAVPMVGAILNTINLRLDTRTISILLRHSESKLIFVDQA 120

Query: 121 SCALISDALALFPSETKRPLLVLIADDTAVQDSSLSSSVLIRNDFVDSYEEMVWKGDSNF 180
           SCALI +ALALFP E KRPLLVLIADD A QDSSL S V IR++FVDSYE+MV +GDS F
Sbjct: 121 SCALIFEALALFPPEMKRPLLVLIADDAAEQDSSLPSFVSIRDNFVDSYEDMVKRGDSKF 180

Query: 181 EWIRPVSEWDPMVLNYTSGTTSSPKGVVHCHRGIFTVALDSLVEWGVPKQSVYLWTLPMF 240
           +WIRP+SEWDP+VLNYTSGTTSSPKGVVHCHRGIFTVALDSL+EWGVPKQSVYLWTLPMF
Sbjct: 181 QWIRPLSEWDPIVLNYTSGTTSSPKGVVHCHRGIFTVALDSLLEWGVPKQSVYLWTLPMF 240

Query: 241 HANGWSFPWGIAAVGGTNVCIRKFDASLIFSLIRRHHVTHLCGAPVVLNMLTNSPDNRPL 300
           H NGWSFPWGIAAVGGTNVCIRKFDA LIFSLIRRHHVTHLCGAPVVLNMLTNSPDN PL
Sbjct: 241 HGNGWSFPWGIAAVGGTNVCIRKFDARLIFSLIRRHHVTHLCGAPVVLNMLTNSPDNLPL 300

Query: 301 DRPVKILTAGAPPPAAVLLRIESLGFVVTHGYGLTESGGTIISCSWKSEWNRLTQTEQAR 360
           DRPVKILTAGAPPPA++LLR ESLGFVVTHGYGLTES GTII CSWKSEWN+L   EQAR
Sbjct: 301 DRPVKILTAGAPPPASILLRTESLGFVVTHGYGLTESAGTIICCSWKSEWNQLPAIEQAR 360

Query: 361 LKARQGVRMLAVEAADVLDPKTRKSVKRDGASIGEIVVRGGSIMLGYLKDPEATAKAMTD 420
           LKARQGVRMLAVEA DVLDP+TRKSVKRDGASIGEIVVRG S+ LGYLKDPE TAKAM++
Sbjct: 361 LKARQGVRMLAVEAVDVLDPETRKSVKRDGASIGEIVVRGASVTLGYLKDPEGTAKAMSE 420

Query: 421 DGWFFTGDVGVMHPDGYIEIKDRSKDVIISGGENLSSVEVESVLYMNPVVNEVAVVARPD 480
           DGWFFTGDVGVMHPDGY+EIKDRSKDVIISGGENLSSVEVESVLYMNP+VNE AVVARPD
Sbjct: 421 DGWFFTGDVGVMHPDGYLEIKDRSKDVIISGGENLSSVEVESVLYMNPMVNEAAVVARPD 480

Query: 481 EFWGETPCAFVNLKKGLTRQPTEEEIIEFCKGKLPRFMVPKTVVFTTELPKTSTGKILKS 540
           EFWGETPCAF++LK+GL+R+PTEEEIIE+CKGKLPRFMVPKTV+F TE+PKT+TGKI K 
Sbjct: 481 EFWGETPCAFISLKEGLSRKPTEEEIIEYCKGKLPRFMVPKTVIFMTEMPKTATGKIQKF 540

Query: 541 VLREMAKSMGPTPKSRL 558
           VLR+ AKS+GP PKSRL
Sbjct: 541 VLRQKAKSLGPAPKSRL 557

BLAST of Cla97C01G008675 vs. ExPASy TrEMBL
Match: A0A0A0KMT3 (AMP dependent ligase OS=Cucumis sativus OX=3659 GN=Csa_5G318390 PE=4 SV=1)

HSP 1 Score: 983.8 bits (2542), Expect = 3.0e-283
Identity = 479/557 (86.00%), Postives = 517/557 (92.82%), Query Frame = 0

Query: 1   MENLKPRPANSSPLTPIGFIERTALIYGDSPSIIYNSTTYTWSETHRRCLQLASSLSSIG 60
           ME+LKPRPANS PLTPIGFIER A+IYGDS SIIYNSTTYTWSETHRRCLQLASS+SS G
Sbjct: 1   MEDLKPRPANSFPLTPIGFIERAAIIYGDSTSIIYNSTTYTWSETHRRCLQLASSISSFG 60

Query: 61  IQRGHVVSVIAPNTPAMYELHFAVPMVGAVLNTINLRLDPRTISVLLRHSETKLIFVDQA 120
           IQRGHVVSV APNTPAMYELHFAVPM GA+LNTINLRLD RTIS+LLRHSE+KLIFVDQ 
Sbjct: 61  IQRGHVVSVFAPNTPAMYELHFAVPMAGAILNTINLRLDTRTISILLRHSESKLIFVDQP 120

Query: 121 SCALISDALALFPSETKRPLLVLIADDTAVQDSSLSSSVLIRNDFVDSYEEMVWKGDSNF 180
           SCALI DALALFP + KRPLLVLI DDTA QDSSL S V IR++FVDSYE MV  GDS F
Sbjct: 121 SCALIFDALALFPPDMKRPLLVLITDDTAEQDSSLPSVVSIRDNFVDSYENMVKSGDSKF 180

Query: 181 EWIRPVSEWDPMVLNYTSGTTSSPKGVVHCHRGIFTVALDSLVEWGVPKQSVYLWTLPMF 240
           +WIRP+SEWDP+VLNYTSGTTS+PKGVV+CHRG+FTVALDS++EWGVPKQSVYLWTLPMF
Sbjct: 181 QWIRPLSEWDPIVLNYTSGTTSAPKGVVNCHRGVFTVALDSILEWGVPKQSVYLWTLPMF 240

Query: 241 HANGWSFPWGIAAVGGTNVCIRKFDASLIFSLIRRHHVTHLCGAPVVLNMLTNSPDNRPL 300
           H NGWSFPWGIAAVGGTNVCIRKFD+ LIFSLIRRHHVTHLCGAPVVLNMLTNSPDN PL
Sbjct: 241 HGNGWSFPWGIAAVGGTNVCIRKFDSRLIFSLIRRHHVTHLCGAPVVLNMLTNSPDNLPL 300

Query: 301 DRPVKILTAGAPPPAAVLLRIESLGFVVTHGYGLTESGGTIISCSWKSEWNRLTQTEQAR 360
           DRPVKILTAGAPPPA+VLLR ESLGFVVTHGYGLTES GTII CSWKSEWN+L   EQAR
Sbjct: 301 DRPVKILTAGAPPPASVLLRTESLGFVVTHGYGLTESAGTIICCSWKSEWNQLPAIEQAR 360

Query: 361 LKARQGVRMLAVEAADVLDPKTRKSVKRDGASIGEIVVRGGSIMLGYLKDPEATAKAMTD 420
           LKARQGVRMLAVEA DVLDP+TRKSVKRDGASIGEIVVRG S+MLGYLKD EAT+KAM++
Sbjct: 361 LKARQGVRMLAVEAVDVLDPETRKSVKRDGASIGEIVVRGPSLMLGYLKDTEATSKAMSE 420

Query: 421 DGWFFTGDVGVMHPDGYIEIKDRSKDVIISGGENLSSVEVESVLYMNPVVNEVAVVARPD 480
           DGWFFTGDVGVMHPDGYIEIKDRSKDVIISGGENLSSVEVESVLYMNP+VNE AVVARPD
Sbjct: 421 DGWFFTGDVGVMHPDGYIEIKDRSKDVIISGGENLSSVEVESVLYMNPMVNEAAVVARPD 480

Query: 481 EFWGETPCAFVNLKKGLTRQPTEEEIIEFCKGKLPRFMVPKTVVFTTELPKTSTGKILKS 540
           EFWGETPCAF++LK+GL+R+PTEEEIIE+CKGKLP+FMVPKTV+F TE+PKT+TGK  K 
Sbjct: 481 EFWGETPCAFISLKEGLSRKPTEEEIIEYCKGKLPKFMVPKTVIFMTEMPKTATGKFQKF 540

Query: 541 VLREMAKSMGPTPKSRL 558
           VLR+ AKS+GP  KSRL
Sbjct: 541 VLRQKAKSLGPPQKSRL 557

BLAST of Cla97C01G008675 vs. ExPASy TrEMBL
Match: A0A6J1FL76 (probable acyl-activating enzyme 5, peroxisomal OS=Cucurbita moschata OX=3662 GN=LOC111445243 PE=4 SV=1)

HSP 1 Score: 961.8 bits (2485), Expect = 1.2e-276
Identity = 471/557 (84.56%), Postives = 505/557 (90.66%), Query Frame = 0

Query: 1   MENLKPRPANSSPLTPIGFIERTALIYGDSPSIIYNSTTYTWSETHRRCLQLASSLSSIG 60
           ME+LKP PANSSPLTPIGF+ER A++YGD PSIIYNSTTYTW+ETHRRCLQLASSL SIG
Sbjct: 1   MEDLKPNPANSSPLTPIGFLERAAVVYGDCPSIIYNSTTYTWTETHRRCLQLASSLVSIG 60

Query: 61  IQRGHVVSVIAPNTPAMYELHFAVPMVGAVLNTINLRLDPRTISVLLRHSETKLIFVDQA 120
           IQRG VVSV++PNT AMYELHFAVPM GAVLNTINLRLD RTISV+LRH ET LIFVDQA
Sbjct: 61  IQRGQVVSVVSPNTAAMYELHFAVPMAGAVLNTINLRLDARTISVILRHCETNLIFVDQA 120

Query: 121 SCALISDALALFPSETKRPLLVLIADDTAVQDSSLSSSVLIRNDFVDSYEEMVWKGDSNF 180
           SC LISDALALFP E KRP L+LIAD+ A +DSS +SSV  R DFVDSYEEMV KGD  F
Sbjct: 121 SCGLISDALALFPPEMKRPPLILIADNAAAEDSSSASSVWNRADFVDSYEEMVQKGDPLF 180

Query: 181 EWIRPVSEWDPMVLNYTSGTTSSPKGVVHCHRGIFTVALDSLVEWGVPKQSVYLWTLPMF 240
           +WIRP SEWDP+VLNYTSGTTSSPKGVVHCHRGIFTV LDSL+EWGVPK+SVYLWTLPMF
Sbjct: 181 QWIRPASEWDPIVLNYTSGTTSSPKGVVHCHRGIFTVTLDSLIEWGVPKESVYLWTLPMF 240

Query: 241 HANGWSFPWGIAAVGGTNVCIRKFDASLIFSLIRRHHVTHLCGAPVVLNMLTNSPDNRPL 300
           HANGWSFPWGIAAVGGTNVCIRKFDA+ IF+LI R+HVTHLCGAPVVLNMLTNSPDNRPL
Sbjct: 241 HANGWSFPWGIAAVGGTNVCIRKFDAAAIFALIHRYHVTHLCGAPVVLNMLTNSPDNRPL 300

Query: 301 DRPVKILTAGAPPPAAVLLRIESLGFVVTHGYGLTESGGTIISCSWKSEWNRLTQTEQAR 360
           DRPVKILTAGAPPPAAVL R ESLGF V+HGYGLTE+GG +I CSWKS WNRL   E+AR
Sbjct: 301 DRPVKILTAGAPPPAAVLFRTESLGFDVSHGYGLTETGGLVICCSWKSGWNRLPAAERAR 360

Query: 361 LKARQGVRMLAVEAADVLDPKTRKSVKRDGASIGEIVVRGGSIMLGYLKDPEATAKAMTD 420
           LKARQGVR LA+  ADVLDP+T KSVKRDG SIGEIV+RG S+MLGYLKDPEATAKAMT 
Sbjct: 361 LKARQGVRTLAMAVADVLDPETGKSVKRDGTSIGEIVLRGSSMMLGYLKDPEATAKAMTA 420

Query: 421 DGWFFTGDVGVMHPDGYIEIKDRSKDVIISGGENLSSVEVESVLYMNPVVNEVAVVARPD 480
           +GWFFTGDVGVMHPDGY+EIKDRSKDVIISGGENLSSVEVESVLY NP VNE AVVARPD
Sbjct: 421 EGWFFTGDVGVMHPDGYLEIKDRSKDVIISGGENLSSVEVESVLYTNPAVNEAAVVARPD 480

Query: 481 EFWGETPCAFVNLKKGLTRQPTEEEIIEFCKGKLPRFMVPKTVVFTTELPKTSTGKILKS 540
           EFWGETPCAFV+LK+GLTR+PTE EIIE+C+GKLPRFMVPKTVVFT ELPKTSTGKI K 
Sbjct: 481 EFWGETPCAFVSLKEGLTRRPTEGEIIEYCRGKLPRFMVPKTVVFTAELPKTSTGKIQKF 540

Query: 541 VLREMAKSMGPTPKSRL 558
           VLREMAKSMGP  KSRL
Sbjct: 541 VLREMAKSMGPAGKSRL 557

BLAST of Cla97C01G008675 vs. ExPASy TrEMBL
Match: A0A6J1JTQ2 (probable acyl-activating enzyme 5, peroxisomal OS=Cucurbita maxima OX=3661 GN=LOC111489657 PE=4 SV=1)

HSP 1 Score: 953.0 bits (2462), Expect = 5.6e-274
Identity = 471/561 (83.96%), Postives = 504/561 (89.84%), Query Frame = 0

Query: 1   MENLKPRPANSSPLTPIGFIERTALIYGDSPSIIYNSTTYTWSETHRRCLQLASSLSSIG 60
           ME+LKP PANSSPLTPIGF+ER A++YGD PSIIYNSTTYTW+ETHRRCLQLASSL SIG
Sbjct: 1   MEDLKPNPANSSPLTPIGFLERAAVVYGDCPSIIYNSTTYTWTETHRRCLQLASSLVSIG 60

Query: 61  IQRGHVVSVIAPNTPAMYELHFAVPMVGAVLNTINLRLDPRTISVLLRHSETKLIFVDQA 120
           IQRG VVSV++PNTPAMYELHFAVPM GAVLNTINLRLD RTISV+LRH ET LIFVDQA
Sbjct: 61  IQRGQVVSVVSPNTPAMYELHFAVPMAGAVLNTINLRLDSRTISVILRHCETNLIFVDQA 120

Query: 121 SCALISDALALFPSETKRPLLVLIADDTAVQDSSLS----SSVLIRNDFVDSYEEMVWKG 180
           SC LISDALALFP E KRP L+LIAD+ A +DSS S    SSV  R DFVDSYEEMV KG
Sbjct: 121 SCGLISDALALFPPEMKRPPLILIADNAAAEDSSSSSSSASSVSNRADFVDSYEEMVQKG 180

Query: 181 DSNFEWIRPVSEWDPMVLNYTSGTTSSPKGVVHCHRGIFTVALDSLVEWGVPKQSVYLWT 240
           D  F+WIRPVSEWDP+VLNYTSGTTSSPKGVVHCHRGIFTV LDSL+EWGV KQSVYLWT
Sbjct: 181 DPLFQWIRPVSEWDPIVLNYTSGTTSSPKGVVHCHRGIFTVTLDSLIEWGVSKQSVYLWT 240

Query: 241 LPMFHANGWSFPWGIAAVGGTNVCIRKFDASLIFSLIRRHHVTHLCGAPVVLNMLTNSPD 300
           LPMFHANGWSFPWGIAAVGGTNVCIRKFDA+ IF+LI R+HVTHLCGAPVVLNMLTNSPD
Sbjct: 241 LPMFHANGWSFPWGIAAVGGTNVCIRKFDAATIFALIHRYHVTHLCGAPVVLNMLTNSPD 300

Query: 301 NRPLDRPVKILTAGAPPPAAVLLRIESLGFVVTHGYGLTESGGTIISCSWKSEWNRLTQT 360
           NRPLDRPVKILTAGAPPPAAVL R ESLGF V+HGYGLTE+GG +I CSWKS WNRL   
Sbjct: 301 NRPLDRPVKILTAGAPPPAAVLFRTESLGFDVSHGYGLTETGGLVICCSWKSGWNRLPAA 360

Query: 361 EQARLKARQGVRMLAVEAADVLDPKTRKSVKRDGASIGEIVVRGGSIMLGYLKDPEATAK 420
           E+ARLKARQGVR LA+  ADVL P+T KSVKRDG SIGEIV+RG S+MLGYLKDPEATAK
Sbjct: 361 ERARLKARQGVRTLAMAVADVLCPETGKSVKRDGTSIGEIVLRGSSMMLGYLKDPEATAK 420

Query: 421 AMTDDGWFFTGDVGVMHPDGYIEIKDRSKDVIISGGENLSSVEVESVLYMNPVVNEVAVV 480
           AMT +GWFFTGDVGVMHPDGY+EIKDRSKDVIISGGENLSSVEVESVLY NP VNE AVV
Sbjct: 421 AMTAEGWFFTGDVGVMHPDGYLEIKDRSKDVIISGGENLSSVEVESVLYTNPAVNEAAVV 480

Query: 481 ARPDEFWGETPCAFVNLKKGLTRQPTEEEIIEFCKGKLPRFMVPKTVVFTTELPKTSTGK 540
           ARPDEFWGETPCAFV+LK+GLTR+PTE EIIE+C+GKLPRFMVPKTVVF  ELPKTSTGK
Sbjct: 481 ARPDEFWGETPCAFVSLKEGLTRRPTEGEIIEYCRGKLPRFMVPKTVVFMAELPKTSTGK 540

Query: 541 ILKSVLREMAKSMGPTPKSRL 558
           I K VLREMAKSMGP  +SRL
Sbjct: 541 IQKFVLREMAKSMGPAGRSRL 561

BLAST of Cla97C01G008675 vs. TAIR 10
Match: AT5G16370.1 (acyl activating enzyme 5 )

HSP 1 Score: 759.6 bits (1960), Expect = 1.8e-219
Identity = 368/558 (65.95%), Postives = 456/558 (81.72%), Query Frame = 0

Query: 1   MENLKPRPANSSPLTPIGFIERTALIYGDSPSIIYNS-TTYTWSETHRRCLQLASSLSSI 60
           ME +KP  ANS PLTPIGF+ER A +YGD  SI+Y S T YTW ET+ RCL++ASSLSSI
Sbjct: 1   MEQMKPCAANSPPLTPIGFLERAATVYGDCTSIVYGSNTVYTWRETNLRCLRVASSLSSI 60

Query: 61  GIQRGHVVSVIAPNTPAMYELHFAVPMVGAVLNTINLRLDPRTISVLLRHSETKLIFVDQ 120
           GI R  VVSV++PNTPAMYEL FAVPM GA+LN IN RLD RT+SVLLRH  +KL+FVD 
Sbjct: 61  GIGRSDVVSVLSPNTPAMYELQFAVPMSGAILNNINTRLDARTVSVLLRHCGSKLLFVDV 120

Query: 121 ASCALISDALALFPSETKRPLLVLIADDTAVQDSSLSSSVLIRNDFVDSYEEMVWKGDSN 180
            S  L  +A+++    T  P+LV IAD    ++    + V  R  F  +Y++++ +GD +
Sbjct: 121 FSVDLAVEAISMM--TTDPPILVFIADK---EEEGGDADVADRTKFSYTYDDLIHRGDLD 180

Query: 181 FEWIRPVSEWDPMVLNYTSGTTSSPKGVVHCHRGIFTVALDSLVEWGVPKQSVYLWTLPM 240
           F+WIRP SEWDP+VLNYTSGTTS+PKGVVHCHRGIF +++DSL++W VPK  VYLWTLP+
Sbjct: 181 FKWIRPESEWDPVVLNYTSGTTSAPKGVVHCHRGIFVMSIDSLIDWTVPKNPVYLWTLPI 240

Query: 241 FHANGWSFPWGIAAVGGTNVCIRKFDASLIFSLIRRHHVTHLCGAPVVLNMLTNSPDNRP 300
           FHANGWS+PWGIAAVGGTNVC+RKFDA LI+ LIR H VTH+CGAPVVLNML+ + + +P
Sbjct: 241 FHANGWSYPWGIAAVGGTNVCLRKFDAPLIYRLIRDHGVTHMCGAPVVLNMLSATNEFQP 300

Query: 301 LDRPVKILTAGAPPPAAVLLRIESLGFVVTHGYGLTESGGTIISCSWKSEWNRLTQTEQA 360
           L+RPV ILTAGAPPPAAVLLR ES+GFV++HGYGLTE+ G  +SC+WK +WNRL  +++A
Sbjct: 301 LNRPVNILTAGAPPPAAVLLRAESIGFVISHGYGLTETAGLNVSCAWKPQWNRLPASDRA 360

Query: 361 RLKARQGVRMLAVEAADVLDPKTRKSVKRDGASIGEIVVRGGSIMLGYLKDPEATAKAMT 420
           RLKARQGVR +     DV+DP++ +SV+R+G ++GEIV+RG SIMLGYLKDP  T KA+ 
Sbjct: 361 RLKARQGVRTVGFTEIDVVDPESGRSVERNGETVGEIVMRGSSIMLGYLKDPVGTEKAL- 420

Query: 421 DDGWFFTGDVGVMHPDGYIEIKDRSKDVIISGGENLSSVEVESVLYMNPVVNEVAVVARP 480
            +GWF+TGDVGV+H DGY+EIKDRSKD+II+GGEN+SSVEVE+VLY NP VNEVAVVARP
Sbjct: 421 KNGWFYTGDVGVIHSDGYLEIKDRSKDIIITGGENVSSVEVETVLYTNPAVNEVAVVARP 480

Query: 481 DEFWGETPCAFVNLKKGLTRQPTEEEIIEFCKGKLPRFMVPKTVVFTTELPKTSTGKILK 540
           D FWGETPCAFV+LK GLT++PTE E+IE+C+ K+P++MVPKTV F  ELPKTSTGK++K
Sbjct: 481 DVFWGETPCAFVSLKSGLTQRPTEVEMIEYCRKKMPKYMVPKTVSFVDELPKTSTGKVMK 540

Query: 541 SVLREMAKSMGPTPKSRL 558
            VLRE+AK MG T  SR+
Sbjct: 541 FVLREIAKKMGTTRLSRM 552

BLAST of Cla97C01G008675 vs. TAIR 10
Match: AT5G16340.1 (AMP-dependent synthetase and ligase family protein )

HSP 1 Score: 740.0 bits (1909), Expect = 1.4e-213
Identity = 355/551 (64.43%), Postives = 448/551 (81.31%), Query Frame = 0

Query: 1   MENLKPRPANSSPLTPIGFIERTALIYGDSPSIIYNS-TTYTWSETHRRCLQLASSLSSI 60
           ME +KP  ANS PLTPIGF+ER A +YGD  SI+Y S T YTW ET+ RCL++ASSLSSI
Sbjct: 1   MEEMKPCAANSPPLTPIGFLERAATVYGDCTSIVYGSNTVYTWRETNLRCLRVASSLSSI 60

Query: 61  GIQRGHVVSVIAPNTPAMYELHFAVPMVGAVLNTINLRLDPRTISVLLRHSETKLIFVDQ 120
           GI R  VVSV++PNTPAMYEL FAVPM GA+LN IN RLD RT+SVLLRH E+KL+FVD 
Sbjct: 61  GIGRSDVVSVLSPNTPAMYELQFAVPMSGAILNNINTRLDARTVSVLLRHCESKLLFVDV 120

Query: 121 ASCALISDALALFPSETKRPLLVLIADDTAVQDSSLSSSVLIRNDFVDSYEEMVWKGDSN 180
            S  L  +A+++    T  P+LV+IAD    ++    + V   + F  +Y++++ +GD  
Sbjct: 121 FSVDLAVEAVSMM--TTDPPILVVIADK---EEEGGVADVADLSKFSYTYDDLIERGDPG 180

Query: 181 FEWIRPVSEWDPMVLNYTSGTTSSPKGVVHCHRGIFTVALDSLVEWGVPKQSVYLWTLPM 240
           F+WIRP SEWDP+VLNYTSGTTS+PKGVVHCHRGIF +++DSL++W VPK  VYLWTLP+
Sbjct: 181 FKWIRPESEWDPVVLNYTSGTTSAPKGVVHCHRGIFVMSVDSLIDWAVPKNPVYLWTLPI 240

Query: 241 FHANGWSFPWGIAAVGGTNVCIRKFDASLIFSLIRRHHVTHLCGAPVVLNMLTNSPDNRP 300
           FH+NGW+ PWGIAAVGGTNVC+RKFDA LI+ LIR H VTH+CGAPVVLNML+ + +++P
Sbjct: 241 FHSNGWTNPWGIAAVGGTNVCLRKFDAPLIYRLIRDHGVTHMCGAPVVLNMLSATQESQP 300

Query: 301 LDRPVKILTAGAPPPAAVLLRIESLGFVVTHGYGLTESGGTIISCSWKSEWNRLTQTEQA 360
           L+ PV ILTAG+PPPA VLLR ES+GFV++HGYGLTE+ G I+SC+WK +WN L  +++A
Sbjct: 301 LNHPVNILTAGSPPPATVLLRAESIGFVISHGYGLTETAGVIVSCAWKPKWNHLPASDRA 360

Query: 361 RLKARQGVRMLAVEAADVLDPKTRKSVKRDGASIGEIVVRGGSIMLGYLKDPEATAKAMT 420
           RLKARQGVR +     DV+DP++  SV+R+G ++GEIV+RG S+MLGYLKDP  T KA+ 
Sbjct: 361 RLKARQGVRTVGFTEIDVVDPESGLSVERNGETVGEIVMRGSSVMLGYLKDPVGTEKAL- 420

Query: 421 DDGWFFTGDVGVMHPDGYIEIKDRSKDVIISGGENLSSVEVESVLYMNPVVNEVAVVARP 480
            +GWF+TGDVGV+H DGY+EIKDRSKD+II+GGEN+SSVEVE+VLY  P VNEVAVVARP
Sbjct: 421 KNGWFYTGDVGVIHSDGYLEIKDRSKDIIITGGENVSSVEVETVLYTIPAVNEVAVVARP 480

Query: 481 DEFWGETPCAFVNLKKGLTRQPTEEEIIEFCKGKLPRFMVPKTVVFTTELPKTSTGKILK 540
           DEFWGETPCAFV+LK G + +PTEEE++E+C+ K+P++MVPKTV F  ELPK+STGK+ K
Sbjct: 481 DEFWGETPCAFVSLKNGFSGKPTEEELMEYCRKKMPKYMVPKTVSFMDELPKSSTGKVTK 540

Query: 541 SVLREMAKSMG 551
            VLR++AK MG
Sbjct: 541 FVLRDIAKKMG 545

BLAST of Cla97C01G008675 vs. TAIR 10
Match: AT1G75960.1 (AMP-dependent synthetase and ligase family protein )

HSP 1 Score: 714.9 bits (1844), Expect = 5.0e-206
Identity = 350/551 (63.52%), Postives = 437/551 (79.31%), Query Frame = 0

Query: 1   MENLKPRPANSSPLTPIGFIERTALIYGDSPSIIY-NSTTYTWSETHRRCLQLASSLSSI 60
           ME+LKP  ANS PLT +GF+ER A +YGD  SI+Y NST YTW ET+ RCL +AS+LSSI
Sbjct: 1   MEDLKPSAANSLPLTLLGFLERAATVYGDCTSIVYGNSTVYTWRETNHRCLCVASALSSI 60

Query: 61  GIQRGHVVSVIAPNTPAMYELHFAVPMVGAVLNTINLRLDPRTISVLLRHSETKLIFVDQ 120
           GI R  VVSV++ NTP MYEL F+VPM GA+LN IN RLD RT+SVLLRH E+KL+FVD 
Sbjct: 61  GIGRSDVVSVLSANTPEMYELQFSVPMSGAILNNINTRLDARTVSVLLRHCESKLLFVDF 120

Query: 121 ASCALISDALALFPSETKRPLLVLIADDTAVQDSSLSSSVLIRNDFVDSYEEMVWKGDSN 180
               L  +A+ +       P+LVLIA++   ++    + V  R+ F   Y +++ +G+ +
Sbjct: 121 FYSDLAVEAITML---LNPPILVLIANE---EEEEGGAEVTERSKFCYLYSDLITRGNPD 180

Query: 181 FEWIRPVSEWDPMVLNYTSGTTSSPKGVVHCHRGIFTVALDSLVEWGVPKQSVYLWTLPM 240
           F+WIRP SEWDP+V+NYTSGTTSSPKGVVHCHRGIF + LDSL +W VPK  VYLWTLP+
Sbjct: 181 FKWIRPGSEWDPIVVNYTSGTTSSPKGVVHCHRGIFVMTLDSLTDWAVPKTPVYLWTLPI 240

Query: 241 FHANGWSFPWGIAAVGGTNVCIRKFDASLIFSLIRRHHVTHLCGAPVVLNMLTNSPD-NR 300
           FHANGW++PWGIAAVGGTNVC+RK  A  I+ LIR H VTH+ GAP+VL +L+ S + ++
Sbjct: 241 FHANGWTYPWGIAAVGGTNVCVRKLHAPSIYHLIRDHGVTHMYGAPIVLQILSASQESDQ 300

Query: 301 PLDRPVKILTAGAPPPAAVLLRIESLGFVVTHGYGLTESGGTIISCSWKSEWNRLTQTEQ 360
           PL  PV  LTAG+ PPA VLLR ESLGF+V+HGYGLTE+ G I+SC+WK  WNRL  ++Q
Sbjct: 301 PLKSPVNFLTAGSSPPATVLLRAESLGFIVSHGYGLTETAGVIVSCAWKPNWNRLPASDQ 360

Query: 361 ARLKARQGVRMLAVEAADVLDPKTRKSVKRDGASIGEIVVRGGSIMLGYLKDPEATAKAM 420
           A+LK+RQGVR +     DV+DP++ +SV+RDG ++GEIV+RG SIMLGYLK+P  T  + 
Sbjct: 361 AQLKSRQGVRTVGFSEIDVVDPESGRSVERDGETVGEIVLRGSSIMLGYLKNPIGTQNSF 420

Query: 421 TDDGWFFTGDVGVMHPDGYIEIKDRSKDVIISGGENLSSVEVESVLYMNPVVNEVAVVAR 480
             +GWFFTGD+GV+H DGY+EIKDRSKDVIISGGEN+SSVEVE+VLY NP VNE AVVAR
Sbjct: 421 -KNGWFFTGDLGVIHGDGYLEIKDRSKDVIISGGENVSSVEVEAVLYTNPAVNEAAVVAR 480

Query: 481 PDEFWGETPCAFVNLKKGLTRQPTEEEIIEFCKGKLPRFMVPKTVVFTTELPKTSTGKIL 540
           PDEFWGETPCAFV+LK GLTR+PT++EIIE+CK K+PR+M PKTV F  ELPKTSTGKI+
Sbjct: 481 PDEFWGETPCAFVSLKPGLTRKPTDKEIIEYCKYKMPRYMAPKTVSFLEELPKTSTGKII 540

Query: 541 KSVLREMAKSM 550
           KS+L+E+AK+M
Sbjct: 541 KSLLKEIAKNM 544

BLAST of Cla97C01G008675 vs. TAIR 10
Match: AT1G21540.1 (AMP-dependent synthetase and ligase family protein )

HSP 1 Score: 704.9 bits (1818), Expect = 5.1e-203
Identity = 340/557 (61.04%), Postives = 434/557 (77.92%), Query Frame = 0

Query: 1   MENLKPRPANSSPLTPIGFIERTALIYGDSPSIIY-NSTTYTWSETHRRCLQLASSL--S 60
           ME L P P+NS+PLT +GF++R A +YGD PSI++  +T +TWSETH RCL++AS+L  S
Sbjct: 1   MELLLPHPSNSTPLTVLGFLDRAASVYGDCPSILHTTNTVHTWSETHNRCLRIASALTSS 60

Query: 61  SIGIQRGHVVSVIAPNTPAMYELHFAVPMVGAVLNTINLRLDPRTISVLLRHSETKLIFV 120
           S+GI RG VVSV+ PN P++YEL FAVPM GA+LN IN RLD   +SVLLRHSE+KL+FV
Sbjct: 61  SLGINRGQVVSVVGPNVPSVYELQFAVPMSGAILNNINPRLDAHALSVLLRHSESKLVFV 120

Query: 121 DQASCALISDALALFPSETKRPLLVLIADDTAVQDSSLSSSVLIRNDFVDSYEEMVWKGD 180
           D  S +++ +A++ F  + ++P LVL+ DD   +D SLS S    +DF+D+Y+ ++ +GD
Sbjct: 121 DPNSISVVLEAVS-FMRQNEKPHLVLLDDDQ--EDGSLSPSA--ASDFLDTYQGVMERGD 180

Query: 181 SNFEWIRPVSEWDPMVLNYTSGTTSSPKGVVHCHRGIFTVALDSLVEWGVPKQSVYLWTL 240
           S F+WIRP +EW PM+LNYTSGTTSSPKGVV  HR IF + + SL++W  P + VYLWTL
Sbjct: 181 SRFKWIRPQTEWQPMILNYTSGTTSSPKGVVLSHRAIFMLTVSSLLDWHFPNRPVYLWTL 240

Query: 241 PMFHANGWSFPWGIAAVGGTNVCIRKFDASLIFSLIRRHHVTHLCGAPVVLNMLTNSPDN 300
           PMFHANGW + WG AAVG TNVC R+ DA  I+ LI +HHVTH+C AP+VLNMLTN P  
Sbjct: 241 PMFHANGWGYTWGTAAVGATNVCTRRVDAPTIYDLIDKHHVTHMCAAPMVLNMLTNYPSR 300

Query: 301 RPLDRPVKILTAGAPPPAAVLLRIESLGFVVTHGYGLTESGGTIISCSWKSEWNRLTQTE 360
           +PL  PV+++TAGAPPPAA++ R E+LGF V HGYGLTE+GG ++SC+WK+EW+ L   E
Sbjct: 301 KPLKNPVQVMTAGAPPPAAIISRAETLGFNVGHGYGLTETGGPVVSCAWKAEWDHLDPLE 360

Query: 361 QARLKARQGVRMLAVEAADVLDPKTRKSVKRDGASIGEIVVRGGSIMLGYLKDPEATAKA 420
           +ARLK+RQGVR +     DV DP+T KSV+ DG S+GEIV++GGS+MLGY KDPE TA  
Sbjct: 361 RARLKSRQGVRTIGFAEVDVRDPRTGKSVEHDGVSVGEIVLKGGSVMLGYYKDPEGTAAC 420

Query: 421 MTDDGWFFTGDVGVMHPDGYIEIKDRSKDVIISGGENLSSVEVESVLYMNPVVNEVAVVA 480
           M +DGWF++GDVGV+H DGY+E+KDRSKDVII GGEN+SS EVE+VLY NPVV E AVVA
Sbjct: 421 MREDGWFYSGDVGVIHEDGYLEVKDRSKDVIICGGENISSAEVETVLYTNPVVKEAAVVA 480

Query: 481 RPDEFWGETPCAFVNLK-----KGLTRQPTEEEIIEFCKGKLPRFMVPKTVVFTTELPKT 540
           +PD+ WGETPCAFV+LK      GL    TE EI EFCK +LP++MVP+ V+F  ELPKT
Sbjct: 481 KPDKMWGETPCAFVSLKYDSNGNGLV---TEREIREFCKTRLPKYMVPRKVIFQEELPKT 540

Query: 541 STGKILKSVLREMAKSM 550
           STGKI K +LR+MAKS+
Sbjct: 541 STGKIQKFLLRQMAKSL 549

BLAST of Cla97C01G008675 vs. TAIR 10
Match: AT1G77240.1 (AMP-dependent synthetase and ligase family protein )

HSP 1 Score: 693.0 bits (1787), Expect = 2.0e-199
Identity = 329/551 (59.71%), Postives = 422/551 (76.59%), Query Frame = 0

Query: 1   MENLKPRPANSSPLTPIGFIERTALIYGDSPSIIYNSTTYTWSETHRRCLQLASSLS--S 60
           ME L P  +NS PLT +GF+ER A ++GDSPS+++ +T +TWSETH RCL++AS+LS  S
Sbjct: 1   MELLLPHASNSCPLTVLGFLERAASVFGDSPSLLHTTTVHTWSETHSRCLRIASTLSSAS 60

Query: 61  IGIQRGHVVSVIAPNTPAMYELHFAVPMVGAVLNTINLRLDPRTISVLLRHSETKLIFVD 120
           +GI RG VVSVI PN P++YEL FAVPM GAVLN IN RLD   +SVLLRHSE+KL+FVD
Sbjct: 61  LGINRGQVVSVIGPNVPSVYELQFAVPMSGAVLNNINPRLDAHALSVLLRHSESKLVFVD 120

Query: 121 QASCALISDALALFPSETKRPLLVLIADDTAVQDSSLSSSVLIRNDFVDSYEEMVWKGDS 180
             S +L+ +A++  P + +RP LV++ D   +  SS +       DF+D+YE  + +GD 
Sbjct: 121 HHSSSLVLEAVSFLPKD-ERPRLVILNDGNDMPSSSSADM-----DFLDTYEGFMERGDL 180

Query: 181 NFEWIRPVSEWDPMVLNYTSGTTSSPKGVVHCHRGIFTVALDSLVEWGVPKQSVYLWTLP 240
            F+W+RP SEW PMVLNYTSGTTSSPKGVVH HR +F   ++SL++W +P + VYLWTLP
Sbjct: 181 RFKWVRPKSEWTPMVLNYTSGTTSSPKGVVHSHRSVFMSTINSLLDWSLPNRPVYLWTLP 240

Query: 241 MFHANGWSFPWGIAAVGGTNVCIRKFDASLIFSLIRRHHVTHLCGAPVVLNMLTNSPDNR 300
           MFHANGWS+ W  AAVG  N+C+ + D   IF+LI ++ VTH+C AP+VLNMLTN P  +
Sbjct: 241 MFHANGWSYTWATAAVGARNICVTRVDVPTIFNLIDKYQVTHMCAAPMVLNMLTNHPAQK 300

Query: 301 PLDRPVKILTAGAPPPAAVLLRIESLGFVVTHGYGLTESGGTIISCSWKSEWNRLTQTEQ 360
           PL  PVK++TAGAPPPA V+ + E+LGF V+HGYG+TE+GG ++SC+ K EW+RL   E+
Sbjct: 301 PLQSPVKVMTAGAPPPATVISKAEALGFDVSHGYGMTETGGLVVSCALKPEWDRLEPDER 360

Query: 361 ARLKARQGVRMLAVEAADVLDPKTRKSVKRDGASIGEIVVRGGSIMLGYLKDPEATAKAM 420
           A+ K+RQG+R       DV DP + KSVK DGA++GEIV RGGS+MLGY KDPE TA +M
Sbjct: 361 AKQKSRQGIRTAVFAEVDVRDPISGKSVKHDGATVGEIVFRGGSVMLGYYKDPEGTAASM 420

Query: 421 TDDGWFFTGDVGVMHPDGYIEIKDRSKDVIISGGENLSSVEVESVLYMNPVVNEVAVVAR 480
            +DGWF+TGD+GVMHPDGY+E+KDRSKDV+I GGEN+SS E+E+VLY NP + E AVVA+
Sbjct: 421 REDGWFYTGDIGVMHPDGYLEVKDRSKDVVICGGENISSTELEAVLYTNPAIKEAAVVAK 480

Query: 481 PDEFWGETPCAFVNLKKGLTRQPTEEEIIEFCKGKLPRFMVPKTVVFTTELPKTSTGKIL 540
           PD+ WGETPCAFV+LK       TE EI EFCK KLP++MVP+ VVF  ELPKTSTGKI 
Sbjct: 481 PDKMWGETPCAFVSLKYH-DGSVTEREIREFCKTKLPKYMVPRNVVFLEELPKTSTGKIQ 540

Query: 541 KSVLREMAKSM 550
           K +LR+MAKS+
Sbjct: 541 KFLLRQMAKSL 544

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
XP_008463174.14.1e-28787.25PREDICTED: probable acyl-activating enzyme 5, peroxisomal [Cucumis melo] >KAA004... [more]
XP_004148908.16.1e-28386.00probable acyl-activating enzyme 5, peroxisomal [Cucumis sativus] >KGN50898.1 hyp... [more]
XP_022939278.12.5e-27684.56probable acyl-activating enzyme 5, peroxisomal [Cucurbita moschata] >KAG6579057.... [more]
XP_022993747.11.2e-27383.96probable acyl-activating enzyme 5, peroxisomal [Cucurbita maxima][more]
XP_023550909.11.2e-27383.78probable acyl-activating enzyme 5, peroxisomal [Cucurbita pepo subsp. pepo][more]
Match NameE-valueIdentityDescription
M4IQQ55.0e-23568.182-methylpropanoate--CoA ligase CCL4 OS=Humulus lupulus OX=3486 GN=CCL4 PE=1 SV=1[more]
M4ISH21.3e-21965.35Probable CoA ligase CCL11 OS=Humulus lupulus OX=3486 GN=CCL11 PE=2 SV=1[more]
Q9FFE62.5e-21865.95Probable acyl-activating enzyme 5, peroxisomal OS=Arabidopsis thaliana OX=3702 G... [more]
Q9FFE92.0e-21264.43Probable acyl-activating enzyme 6 OS=Arabidopsis thaliana OX=3702 GN=AAE6 PE=2 S... [more]
Q9LQS17.0e-20563.52Probable acyl-activating enzyme 8 OS=Arabidopsis thaliana OX=3702 GN=AAE8 PE=2 S... [more]
Match NameE-valueIdentityDescription
A0A5D3D6232.0e-28787.25Putative acyl-activating enzyme 5, peroxisomal OS=Cucumis melo var. makuwa OX=11... [more]
A0A1S3CIL02.0e-28787.25probable acyl-activating enzyme 5, peroxisomal OS=Cucumis melo OX=3656 GN=LOC103... [more]
A0A0A0KMT33.0e-28386.00AMP dependent ligase OS=Cucumis sativus OX=3659 GN=Csa_5G318390 PE=4 SV=1[more]
A0A6J1FL761.2e-27684.56probable acyl-activating enzyme 5, peroxisomal OS=Cucurbita moschata OX=3662 GN=... [more]
A0A6J1JTQ25.6e-27483.96probable acyl-activating enzyme 5, peroxisomal OS=Cucurbita maxima OX=3661 GN=LO... [more]
Match NameE-valueIdentityDescription
AT5G16370.11.8e-21965.95acyl activating enzyme 5 [more]
AT5G16340.11.4e-21364.43AMP-dependent synthetase and ligase family protein [more]
AT1G75960.15.0e-20663.52AMP-dependent synthetase and ligase family protein [more]
AT1G21540.15.1e-20361.04AMP-dependent synthetase and ligase family protein [more]
AT1G77240.12.0e-19959.71AMP-dependent synthetase and ligase family protein [more]
InterPro
Analysis Name: InterPro Annotations of Watermelon (97103) v2.5
Date Performed: 2022-01-31
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR042099ANL, N-terminal domainGENE3D3.40.50.12780coord: 3..445
e-value: 4.8E-101
score: 340.3
IPR025110AMP-binding enzyme, C-terminal domainPFAMPF13193AMP-binding_Ccoord: 459..536
e-value: 9.7E-23
score: 81.0
NoneNo IPR availableGENE3D3.30.300.30coord: 446..549
e-value: 1.7E-30
score: 107.4
NoneNo IPR availablePANTHERPTHR43859ACYL-ACTIVATING ENZYMEcoord: 2..552
NoneNo IPR availablePANTHERPTHR43859:SF50LONG-CHAIN ACYL-COA SYNTHETASEcoord: 2..552
NoneNo IPR availableCDDcd12118ttLC_FACS_AEE21_likecoord: 12..544
e-value: 0.0
score: 804.981
NoneNo IPR availableSUPERFAMILY56801Acetyl-CoA synthetase-likecoord: 11..548
IPR000873AMP-dependent synthetase/ligasePFAMPF00501AMP-bindingcoord: 23..450
e-value: 1.8E-92
score: 310.2
IPR020845AMP-binding, conserved sitePROSITEPS00455AMP_BINDINGcoord: 194..205

Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
Cla97C01G008675.1Cla97C01G008675.1mRNA


GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
molecular_function GO:0016874 ligase activity