Homology
BLAST of Cla97C01G008675 vs. NCBI nr
Match:
XP_008463174.1 (PREDICTED: probable acyl-activating enzyme 5, peroxisomal [Cucumis melo] >KAA0046220.1 putative acyl-activating enzyme 5, peroxisomal [Cucumis melo var. makuwa] >TYK18976.1 putative acyl-activating enzyme 5, peroxisomal [Cucumis melo var. makuwa])
HSP 1 Score: 997.7 bits (2578), Expect = 4.1e-287
Identity = 486/557 (87.25%), Postives = 521/557 (93.54%), Query Frame = 0
Query: 1 MENLKPRPANSSPLTPIGFIERTALIYGDSPSIIYNSTTYTWSETHRRCLQLASSLSSIG 60
ME+LKP PANS PLTPIGFI+R A IYGDS SIIYNSTTYTWSETHRRCLQLASS+SS G
Sbjct: 1 MEDLKPTPANSFPLTPIGFIQRAATIYGDSTSIIYNSTTYTWSETHRRCLQLASSISSFG 60
Query: 61 IQRGHVVSVIAPNTPAMYELHFAVPMVGAVLNTINLRLDPRTISVLLRHSETKLIFVDQA 120
IQRGHVVSVIAPNTPAMYELHFAVPMVGA+LNTINLRLD RTIS+LLRHSE+KLIFVDQA
Sbjct: 61 IQRGHVVSVIAPNTPAMYELHFAVPMVGAILNTINLRLDTRTISILLRHSESKLIFVDQA 120
Query: 121 SCALISDALALFPSETKRPLLVLIADDTAVQDSSLSSSVLIRNDFVDSYEEMVWKGDSNF 180
SCALI +ALALFP E KRPLLVLIADD A QDSSL S V IR++FVDSYE+MV +GDS F
Sbjct: 121 SCALIFEALALFPPEMKRPLLVLIADDAAEQDSSLPSFVSIRDNFVDSYEDMVKRGDSKF 180
Query: 181 EWIRPVSEWDPMVLNYTSGTTSSPKGVVHCHRGIFTVALDSLVEWGVPKQSVYLWTLPMF 240
+WIRP+SEWDP+VLNYTSGTTSSPKGVVHCHRGIFTVALDSL+EWGVPKQSVYLWTLPMF
Sbjct: 181 QWIRPLSEWDPIVLNYTSGTTSSPKGVVHCHRGIFTVALDSLLEWGVPKQSVYLWTLPMF 240
Query: 241 HANGWSFPWGIAAVGGTNVCIRKFDASLIFSLIRRHHVTHLCGAPVVLNMLTNSPDNRPL 300
H NGWSFPWGIAAVGGTNVCIRKFDA LIFSLIRRHHVTHLCGAPVVLNMLTNSPDN PL
Sbjct: 241 HGNGWSFPWGIAAVGGTNVCIRKFDARLIFSLIRRHHVTHLCGAPVVLNMLTNSPDNLPL 300
Query: 301 DRPVKILTAGAPPPAAVLLRIESLGFVVTHGYGLTESGGTIISCSWKSEWNRLTQTEQAR 360
DRPVKILTAGAPPPA++LLR ESLGFVVTHGYGLTES GTII CSWKSEWN+L EQAR
Sbjct: 301 DRPVKILTAGAPPPASILLRTESLGFVVTHGYGLTESAGTIICCSWKSEWNQLPAIEQAR 360
Query: 361 LKARQGVRMLAVEAADVLDPKTRKSVKRDGASIGEIVVRGGSIMLGYLKDPEATAKAMTD 420
LKARQGVRMLAVEA DVLDP+TRKSVKRDGASIGEIVVRG S+ LGYLKDPE TAKAM++
Sbjct: 361 LKARQGVRMLAVEAVDVLDPETRKSVKRDGASIGEIVVRGASVTLGYLKDPEGTAKAMSE 420
Query: 421 DGWFFTGDVGVMHPDGYIEIKDRSKDVIISGGENLSSVEVESVLYMNPVVNEVAVVARPD 480
DGWFFTGDVGVMHPDGY+EIKDRSKDVIISGGENLSSVEVESVLYMNP+VNE AVVARPD
Sbjct: 421 DGWFFTGDVGVMHPDGYLEIKDRSKDVIISGGENLSSVEVESVLYMNPMVNEAAVVARPD 480
Query: 481 EFWGETPCAFVNLKKGLTRQPTEEEIIEFCKGKLPRFMVPKTVVFTTELPKTSTGKILKS 540
EFWGETPCAF++LK+GL+R+PTEEEIIE+CKGKLPRFMVPKTV+F TE+PKT+TGKI K
Sbjct: 481 EFWGETPCAFISLKEGLSRKPTEEEIIEYCKGKLPRFMVPKTVIFMTEMPKTATGKIQKF 540
Query: 541 VLREMAKSMGPTPKSRL 558
VLR+ AKS+GP PKSRL
Sbjct: 541 VLRQKAKSLGPAPKSRL 557
BLAST of Cla97C01G008675 vs. NCBI nr
Match:
XP_004148908.1 (probable acyl-activating enzyme 5, peroxisomal [Cucumis sativus] >KGN50898.1 hypothetical protein Csa_023091 [Cucumis sativus])
HSP 1 Score: 983.8 bits (2542), Expect = 6.1e-283
Identity = 479/557 (86.00%), Postives = 517/557 (92.82%), Query Frame = 0
Query: 1 MENLKPRPANSSPLTPIGFIERTALIYGDSPSIIYNSTTYTWSETHRRCLQLASSLSSIG 60
ME+LKPRPANS PLTPIGFIER A+IYGDS SIIYNSTTYTWSETHRRCLQLASS+SS G
Sbjct: 1 MEDLKPRPANSFPLTPIGFIERAAIIYGDSTSIIYNSTTYTWSETHRRCLQLASSISSFG 60
Query: 61 IQRGHVVSVIAPNTPAMYELHFAVPMVGAVLNTINLRLDPRTISVLLRHSETKLIFVDQA 120
IQRGHVVSV APNTPAMYELHFAVPM GA+LNTINLRLD RTIS+LLRHSE+KLIFVDQ
Sbjct: 61 IQRGHVVSVFAPNTPAMYELHFAVPMAGAILNTINLRLDTRTISILLRHSESKLIFVDQP 120
Query: 121 SCALISDALALFPSETKRPLLVLIADDTAVQDSSLSSSVLIRNDFVDSYEEMVWKGDSNF 180
SCALI DALALFP + KRPLLVLI DDTA QDSSL S V IR++FVDSYE MV GDS F
Sbjct: 121 SCALIFDALALFPPDMKRPLLVLITDDTAEQDSSLPSVVSIRDNFVDSYENMVKSGDSKF 180
Query: 181 EWIRPVSEWDPMVLNYTSGTTSSPKGVVHCHRGIFTVALDSLVEWGVPKQSVYLWTLPMF 240
+WIRP+SEWDP+VLNYTSGTTS+PKGVV+CHRG+FTVALDS++EWGVPKQSVYLWTLPMF
Sbjct: 181 QWIRPLSEWDPIVLNYTSGTTSAPKGVVNCHRGVFTVALDSILEWGVPKQSVYLWTLPMF 240
Query: 241 HANGWSFPWGIAAVGGTNVCIRKFDASLIFSLIRRHHVTHLCGAPVVLNMLTNSPDNRPL 300
H NGWSFPWGIAAVGGTNVCIRKFD+ LIFSLIRRHHVTHLCGAPVVLNMLTNSPDN PL
Sbjct: 241 HGNGWSFPWGIAAVGGTNVCIRKFDSRLIFSLIRRHHVTHLCGAPVVLNMLTNSPDNLPL 300
Query: 301 DRPVKILTAGAPPPAAVLLRIESLGFVVTHGYGLTESGGTIISCSWKSEWNRLTQTEQAR 360
DRPVKILTAGAPPPA+VLLR ESLGFVVTHGYGLTES GTII CSWKSEWN+L EQAR
Sbjct: 301 DRPVKILTAGAPPPASVLLRTESLGFVVTHGYGLTESAGTIICCSWKSEWNQLPAIEQAR 360
Query: 361 LKARQGVRMLAVEAADVLDPKTRKSVKRDGASIGEIVVRGGSIMLGYLKDPEATAKAMTD 420
LKARQGVRMLAVEA DVLDP+TRKSVKRDGASIGEIVVRG S+MLGYLKD EAT+KAM++
Sbjct: 361 LKARQGVRMLAVEAVDVLDPETRKSVKRDGASIGEIVVRGPSLMLGYLKDTEATSKAMSE 420
Query: 421 DGWFFTGDVGVMHPDGYIEIKDRSKDVIISGGENLSSVEVESVLYMNPVVNEVAVVARPD 480
DGWFFTGDVGVMHPDGYIEIKDRSKDVIISGGENLSSVEVESVLYMNP+VNE AVVARPD
Sbjct: 421 DGWFFTGDVGVMHPDGYIEIKDRSKDVIISGGENLSSVEVESVLYMNPMVNEAAVVARPD 480
Query: 481 EFWGETPCAFVNLKKGLTRQPTEEEIIEFCKGKLPRFMVPKTVVFTTELPKTSTGKILKS 540
EFWGETPCAF++LK+GL+R+PTEEEIIE+CKGKLP+FMVPKTV+F TE+PKT+TGK K
Sbjct: 481 EFWGETPCAFISLKEGLSRKPTEEEIIEYCKGKLPKFMVPKTVIFMTEMPKTATGKFQKF 540
Query: 541 VLREMAKSMGPTPKSRL 558
VLR+ AKS+GP KSRL
Sbjct: 541 VLRQKAKSLGPPQKSRL 557
BLAST of Cla97C01G008675 vs. NCBI nr
Match:
XP_022939278.1 (probable acyl-activating enzyme 5, peroxisomal [Cucurbita moschata] >KAG6579057.1 putative acyl-activating enzyme 5, peroxisomal, partial [Cucurbita argyrosperma subsp. sororia])
HSP 1 Score: 961.8 bits (2485), Expect = 2.5e-276
Identity = 471/557 (84.56%), Postives = 505/557 (90.66%), Query Frame = 0
Query: 1 MENLKPRPANSSPLTPIGFIERTALIYGDSPSIIYNSTTYTWSETHRRCLQLASSLSSIG 60
ME+LKP PANSSPLTPIGF+ER A++YGD PSIIYNSTTYTW+ETHRRCLQLASSL SIG
Sbjct: 1 MEDLKPNPANSSPLTPIGFLERAAVVYGDCPSIIYNSTTYTWTETHRRCLQLASSLVSIG 60
Query: 61 IQRGHVVSVIAPNTPAMYELHFAVPMVGAVLNTINLRLDPRTISVLLRHSETKLIFVDQA 120
IQRG VVSV++PNT AMYELHFAVPM GAVLNTINLRLD RTISV+LRH ET LIFVDQA
Sbjct: 61 IQRGQVVSVVSPNTAAMYELHFAVPMAGAVLNTINLRLDARTISVILRHCETNLIFVDQA 120
Query: 121 SCALISDALALFPSETKRPLLVLIADDTAVQDSSLSSSVLIRNDFVDSYEEMVWKGDSNF 180
SC LISDALALFP E KRP L+LIAD+ A +DSS +SSV R DFVDSYEEMV KGD F
Sbjct: 121 SCGLISDALALFPPEMKRPPLILIADNAAAEDSSSASSVWNRADFVDSYEEMVQKGDPLF 180
Query: 181 EWIRPVSEWDPMVLNYTSGTTSSPKGVVHCHRGIFTVALDSLVEWGVPKQSVYLWTLPMF 240
+WIRP SEWDP+VLNYTSGTTSSPKGVVHCHRGIFTV LDSL+EWGVPK+SVYLWTLPMF
Sbjct: 181 QWIRPASEWDPIVLNYTSGTTSSPKGVVHCHRGIFTVTLDSLIEWGVPKESVYLWTLPMF 240
Query: 241 HANGWSFPWGIAAVGGTNVCIRKFDASLIFSLIRRHHVTHLCGAPVVLNMLTNSPDNRPL 300
HANGWSFPWGIAAVGGTNVCIRKFDA+ IF+LI R+HVTHLCGAPVVLNMLTNSPDNRPL
Sbjct: 241 HANGWSFPWGIAAVGGTNVCIRKFDAAAIFALIHRYHVTHLCGAPVVLNMLTNSPDNRPL 300
Query: 301 DRPVKILTAGAPPPAAVLLRIESLGFVVTHGYGLTESGGTIISCSWKSEWNRLTQTEQAR 360
DRPVKILTAGAPPPAAVL R ESLGF V+HGYGLTE+GG +I CSWKS WNRL E+AR
Sbjct: 301 DRPVKILTAGAPPPAAVLFRTESLGFDVSHGYGLTETGGLVICCSWKSGWNRLPAAERAR 360
Query: 361 LKARQGVRMLAVEAADVLDPKTRKSVKRDGASIGEIVVRGGSIMLGYLKDPEATAKAMTD 420
LKARQGVR LA+ ADVLDP+T KSVKRDG SIGEIV+RG S+MLGYLKDPEATAKAMT
Sbjct: 361 LKARQGVRTLAMAVADVLDPETGKSVKRDGTSIGEIVLRGSSMMLGYLKDPEATAKAMTA 420
Query: 421 DGWFFTGDVGVMHPDGYIEIKDRSKDVIISGGENLSSVEVESVLYMNPVVNEVAVVARPD 480
+GWFFTGDVGVMHPDGY+EIKDRSKDVIISGGENLSSVEVESVLY NP VNE AVVARPD
Sbjct: 421 EGWFFTGDVGVMHPDGYLEIKDRSKDVIISGGENLSSVEVESVLYTNPAVNEAAVVARPD 480
Query: 481 EFWGETPCAFVNLKKGLTRQPTEEEIIEFCKGKLPRFMVPKTVVFTTELPKTSTGKILKS 540
EFWGETPCAFV+LK+GLTR+PTE EIIE+C+GKLPRFMVPKTVVFT ELPKTSTGKI K
Sbjct: 481 EFWGETPCAFVSLKEGLTRRPTEGEIIEYCRGKLPRFMVPKTVVFTAELPKTSTGKIQKF 540
Query: 541 VLREMAKSMGPTPKSRL 558
VLREMAKSMGP KSRL
Sbjct: 541 VLREMAKSMGPAGKSRL 557
BLAST of Cla97C01G008675 vs. NCBI nr
Match:
XP_022993747.1 (probable acyl-activating enzyme 5, peroxisomal [Cucurbita maxima])
HSP 1 Score: 953.0 bits (2462), Expect = 1.2e-273
Identity = 471/561 (83.96%), Postives = 504/561 (89.84%), Query Frame = 0
Query: 1 MENLKPRPANSSPLTPIGFIERTALIYGDSPSIIYNSTTYTWSETHRRCLQLASSLSSIG 60
ME+LKP PANSSPLTPIGF+ER A++YGD PSIIYNSTTYTW+ETHRRCLQLASSL SIG
Sbjct: 1 MEDLKPNPANSSPLTPIGFLERAAVVYGDCPSIIYNSTTYTWTETHRRCLQLASSLVSIG 60
Query: 61 IQRGHVVSVIAPNTPAMYELHFAVPMVGAVLNTINLRLDPRTISVLLRHSETKLIFVDQA 120
IQRG VVSV++PNTPAMYELHFAVPM GAVLNTINLRLD RTISV+LRH ET LIFVDQA
Sbjct: 61 IQRGQVVSVVSPNTPAMYELHFAVPMAGAVLNTINLRLDSRTISVILRHCETNLIFVDQA 120
Query: 121 SCALISDALALFPSETKRPLLVLIADDTAVQDSSLS----SSVLIRNDFVDSYEEMVWKG 180
SC LISDALALFP E KRP L+LIAD+ A +DSS S SSV R DFVDSYEEMV KG
Sbjct: 121 SCGLISDALALFPPEMKRPPLILIADNAAAEDSSSSSSSASSVSNRADFVDSYEEMVQKG 180
Query: 181 DSNFEWIRPVSEWDPMVLNYTSGTTSSPKGVVHCHRGIFTVALDSLVEWGVPKQSVYLWT 240
D F+WIRPVSEWDP+VLNYTSGTTSSPKGVVHCHRGIFTV LDSL+EWGV KQSVYLWT
Sbjct: 181 DPLFQWIRPVSEWDPIVLNYTSGTTSSPKGVVHCHRGIFTVTLDSLIEWGVSKQSVYLWT 240
Query: 241 LPMFHANGWSFPWGIAAVGGTNVCIRKFDASLIFSLIRRHHVTHLCGAPVVLNMLTNSPD 300
LPMFHANGWSFPWGIAAVGGTNVCIRKFDA+ IF+LI R+HVTHLCGAPVVLNMLTNSPD
Sbjct: 241 LPMFHANGWSFPWGIAAVGGTNVCIRKFDAATIFALIHRYHVTHLCGAPVVLNMLTNSPD 300
Query: 301 NRPLDRPVKILTAGAPPPAAVLLRIESLGFVVTHGYGLTESGGTIISCSWKSEWNRLTQT 360
NRPLDRPVKILTAGAPPPAAVL R ESLGF V+HGYGLTE+GG +I CSWKS WNRL
Sbjct: 301 NRPLDRPVKILTAGAPPPAAVLFRTESLGFDVSHGYGLTETGGLVICCSWKSGWNRLPAA 360
Query: 361 EQARLKARQGVRMLAVEAADVLDPKTRKSVKRDGASIGEIVVRGGSIMLGYLKDPEATAK 420
E+ARLKARQGVR LA+ ADVL P+T KSVKRDG SIGEIV+RG S+MLGYLKDPEATAK
Sbjct: 361 ERARLKARQGVRTLAMAVADVLCPETGKSVKRDGTSIGEIVLRGSSMMLGYLKDPEATAK 420
Query: 421 AMTDDGWFFTGDVGVMHPDGYIEIKDRSKDVIISGGENLSSVEVESVLYMNPVVNEVAVV 480
AMT +GWFFTGDVGVMHPDGY+EIKDRSKDVIISGGENLSSVEVESVLY NP VNE AVV
Sbjct: 421 AMTAEGWFFTGDVGVMHPDGYLEIKDRSKDVIISGGENLSSVEVESVLYTNPAVNEAAVV 480
Query: 481 ARPDEFWGETPCAFVNLKKGLTRQPTEEEIIEFCKGKLPRFMVPKTVVFTTELPKTSTGK 540
ARPDEFWGETPCAFV+LK+GLTR+PTE EIIE+C+GKLPRFMVPKTVVF ELPKTSTGK
Sbjct: 481 ARPDEFWGETPCAFVSLKEGLTRRPTEGEIIEYCRGKLPRFMVPKTVVFMAELPKTSTGK 540
Query: 541 ILKSVLREMAKSMGPTPKSRL 558
I K VLREMAKSMGP +SRL
Sbjct: 541 IQKFVLREMAKSMGPAGRSRL 561
BLAST of Cla97C01G008675 vs. NCBI nr
Match:
XP_023550909.1 (probable acyl-activating enzyme 5, peroxisomal [Cucurbita pepo subsp. pepo])
HSP 1 Score: 953.0 bits (2462), Expect = 1.2e-273
Identity = 470/561 (83.78%), Postives = 505/561 (90.02%), Query Frame = 0
Query: 1 MENLKPRPANSSPLTPIGFIERTALIYGDSPSIIYNSTTYTWSETHRRCLQLASSLSSIG 60
ME+LKP PANSSPLTPIGF+ER A++YGD SIIY+STTYTW+ETHRRCLQLASSL SIG
Sbjct: 1 MEDLKPNPANSSPLTPIGFLERAAVVYGDCRSIIYDSTTYTWTETHRRCLQLASSLVSIG 60
Query: 61 IQRGHVVSVIAPNTPAMYELHFAVPMVGAVLNTINLRLDPRTISVLLRHSETKLIFVDQA 120
IQRG VVSV++PNT AMYELHFAVPM GAVLNTINLRLD RTISV+LRH ET LIFVDQA
Sbjct: 61 IQRGQVVSVVSPNTAAMYELHFAVPMAGAVLNTINLRLDARTISVILRHCETNLIFVDQA 120
Query: 121 SCALISDALALFPSETKRPLLVLIADDTAVQDSSLS----SSVLIRNDFVDSYEEMVWKG 180
SC LISDALALFP E KRP L+LIAD+ A +DSS S SSVL R DFVDSYEEMV KG
Sbjct: 121 SCGLISDALALFPPEMKRPPLILIADNAAAEDSSSSSSSASSVLNRADFVDSYEEMVQKG 180
Query: 181 DSNFEWIRPVSEWDPMVLNYTSGTTSSPKGVVHCHRGIFTVALDSLVEWGVPKQSVYLWT 240
D F+WIRP SEWDP+VLNYTSGTTSSPKGVVHCHRGIFTV LDSL+EWGVPK+SVYLWT
Sbjct: 181 DPLFQWIRPASEWDPIVLNYTSGTTSSPKGVVHCHRGIFTVTLDSLIEWGVPKESVYLWT 240
Query: 241 LPMFHANGWSFPWGIAAVGGTNVCIRKFDASLIFSLIRRHHVTHLCGAPVVLNMLTNSPD 300
LPMFHANGWSFPWGIAAVGGTNVCIRKFDA+ IF+LI R+HVTHLCGAPVVLNMLTNSPD
Sbjct: 241 LPMFHANGWSFPWGIAAVGGTNVCIRKFDAAAIFALIHRYHVTHLCGAPVVLNMLTNSPD 300
Query: 301 NRPLDRPVKILTAGAPPPAAVLLRIESLGFVVTHGYGLTESGGTIISCSWKSEWNRLTQT 360
NRPLDRPVKILTAGAPPPAAVL R ESLGF V+HGYGLTE+GG +I CSWKS WNRL
Sbjct: 301 NRPLDRPVKILTAGAPPPAAVLFRTESLGFDVSHGYGLTETGGLVICCSWKSGWNRLPAA 360
Query: 361 EQARLKARQGVRMLAVEAADVLDPKTRKSVKRDGASIGEIVVRGGSIMLGYLKDPEATAK 420
++ARLKARQGVR LA+ ADVLDP+T KSVKRDG SIGEIV+RG S+MLGYLKDPEATAK
Sbjct: 361 DRARLKARQGVRTLAMAVADVLDPETGKSVKRDGTSIGEIVLRGSSMMLGYLKDPEATAK 420
Query: 421 AMTDDGWFFTGDVGVMHPDGYIEIKDRSKDVIISGGENLSSVEVESVLYMNPVVNEVAVV 480
AMT +GWFFTGDVGVMHPDGY+EIKDRSKDVIISGGENLSSVEVESVLY NP VNE AVV
Sbjct: 421 AMTAEGWFFTGDVGVMHPDGYLEIKDRSKDVIISGGENLSSVEVESVLYTNPAVNEAAVV 480
Query: 481 ARPDEFWGETPCAFVNLKKGLTRQPTEEEIIEFCKGKLPRFMVPKTVVFTTELPKTSTGK 540
ARPDEFWGETPCAFV+LK+GLTR+PTE EIIE+C+GKLPRFMVPKTVVFT ELPKTSTGK
Sbjct: 481 ARPDEFWGETPCAFVSLKEGLTRRPTEGEIIEYCRGKLPRFMVPKTVVFTAELPKTSTGK 540
Query: 541 ILKSVLREMAKSMGPTPKSRL 558
I K VLREMAKSMGP KSRL
Sbjct: 541 IQKFVLREMAKSMGPAGKSRL 561
BLAST of Cla97C01G008675 vs. ExPASy Swiss-Prot
Match:
M4IQQ5 (2-methylpropanoate--CoA ligase CCL4 OS=Humulus lupulus OX=3486 GN=CCL4 PE=1 SV=1)
HSP 1 Score: 815.1 bits (2104), Expect = 5.0e-235
Identity = 375/550 (68.18%), Postives = 466/550 (84.73%), Query Frame = 0
Query: 1 MENLKPRPANSSPLTPIGFIERTALIYGDSPSIIYNSTTYTWSETHRRCLQLASSLSSIG 60
ME+LKPRPA+SSPLTP+GF+ER A +YGD S++Y++ +YTWS+THRRCL LASS++S+G
Sbjct: 1 MEDLKPRPASSSPLTPLGFLERAATVYGDCTSVVYDAVSYTWSQTHRRCLCLASSIASLG 60
Query: 61 IQRGHVVSVIAPNTPAMYELHFAVPMVGAVLNTINLRLDPRTISVLLRHSETKLIFVDQA 120
I+ GHVVSV+APN P MYELHFAVPM GA+LN +NLRLD RTIS+LL HSE+KLIFVD
Sbjct: 61 IENGHVVSVLAPNVPQMYELHFAVPMAGAILNAVNLRLDARTISILLHHSESKLIFVDHL 120
Query: 121 SCALISDALALFPSETKRPLLVLIADDTAVQDSSLSSSVLIRNDFVDSYEEMVWKGDSNF 180
S LI +A+ALFP + P LV +AD +S +S + +F SY++++ +GD +F
Sbjct: 121 SRDLILEAIALFPKQAPVPRLVFMAD-----ESESGNSSELGKEFFCSYKDLIDRGDPDF 180
Query: 181 EWIRPVSEWDPMVLNYTSGTTSSPKGVVHCHRGIFTVALDSLVEWGVPKQSVYLWTLPMF 240
+W+ P SEWDPM+LNYTSGTTSSPKGVVHCHRGIF + +DSL++WGVPKQ VYLWTLPMF
Sbjct: 181 KWVMPKSEWDPMILNYTSGTTSSPKGVVHCHRGIFIMTVDSLIDWGVPKQPVYLWTLPMF 240
Query: 241 HANGWSFPWGIAAVGGTNVCIRKFDASLIFSLIRRHHVTHLCGAPVVLNMLTNSPDNRPL 300
HANGWS+PWG+AAVGGTN+C+RKFD+ +I+ +I+RH VTH+CGAPVVLNML+N+P + PL
Sbjct: 241 HANGWSYPWGMAAVGGTNICLRKFDSEIIYDMIKRHGVTHMCGAPVVLNMLSNAPGSEPL 300
Query: 301 DRPVKILTAGAPPPAAVLLRIESLGFVVTHGYGLTESGGTIISCSWKSEWNRLTQTEQAR 360
V+I+TAGAPPP+AVL R ESLGF V+HGYGLTE+ G ++SC+WK EWN L TE+AR
Sbjct: 301 KTTVQIMTAGAPPPSAVLFRTESLGFAVSHGYGLTETAGLVVSCAWKKEWNHLPATERAR 360
Query: 361 LKARQGVRMLAVEAADVLDPKTRKSVKRDGASIGEIVVRGGSIMLGYLKDPEATAKAMTD 420
LK+RQGV + DV+DP T +VKRDG+++GE+V+RGGS+MLGYLKDPE TAK+MT
Sbjct: 361 LKSRQGVGTVMQTKIDVVDPVTGAAVKRDGSTLGEVVLRGGSVMLGYLKDPEGTAKSMTA 420
Query: 421 DGWFFTGDVGVMHPDGYIEIKDRSKDVIISGGENLSSVEVESVLYMNPVVNEVAVVARPD 480
DGWF+TGDVGVMHPDGY+EIKDRSKDVIISGGENLSSVEVES+LY +P + E AVVARPD
Sbjct: 421 DGWFYTGDVGVMHPDGYLEIKDRSKDVIISGGENLSSVEVESILYSHPDILEAAVVARPD 480
Query: 481 EFWGETPCAFVNLKKGLTRQPTEEEIIEFCKGKLPRFMVPKTVVFTTELPKTSTGKILKS 540
EFWGETPCAFV+LKKGLT++PTE+EI+E+C+ KLPR+MVPKTVVF ELPKTSTGK+ K
Sbjct: 481 EFWGETPCAFVSLKKGLTKKPTEKEIVEYCRSKLPRYMVPKTVVFKEELPKTSTGKVQKF 540
Query: 541 VLREMAKSMG 551
+LR+MA+ MG
Sbjct: 541 ILRDMARGMG 545
BLAST of Cla97C01G008675 vs. ExPASy Swiss-Prot
Match:
M4ISH2 (Probable CoA ligase CCL11 OS=Humulus lupulus OX=3486 GN=CCL11 PE=2 SV=1)
HSP 1 Score: 763.8 bits (1971), Expect = 1.3e-219
Identity = 364/557 (65.35%), Postives = 448/557 (80.43%), Query Frame = 0
Query: 1 MENLKPRPANSSPLTPIGFIERTALIYGDSPSIIYNSTTYTWSETHRRCLQLASSLSS-I 60
ME LKPR ANS PLTP+GF+ER A +YGD S++Y+ +YTWS+TH RCL++AS + S +
Sbjct: 1 MEELKPRFANSCPLTPLGFLERAATVYGDCISVVYDDLSYTWSQTHTRCLRVASCIESCL 60
Query: 61 GIQRGHVVSVIAPNTPAMYELHFAVPMVGAVLNTINLRLDPRTISVLLRHSETKLIFVDQ 120
G+++G VVSV+APN PAMYEL+FAVPM G VLN IN RL+ TISV+LRHSE+KL+FVDQ
Sbjct: 61 GVKKGQVVSVVAPNVPAMYELNFAVPMAGVVLNNINTRLNAVTISVMLRHSESKLVFVDQ 120
Query: 121 ASCALISDALALFPSETKRPLLVLIADDTAVQDSSLSSSVLIRNDFVDSYEEMVWKG-DS 180
S L+ DA++LFP T PLLVLIAD+ ++ ++ R FV YE++V KG D
Sbjct: 121 LSVRLVLDAVSLFPKNTPTPLLVLIADNITGENLTVED----REHFVCCYEDLVEKGDDK 180
Query: 181 NFEWIRPVSEWDPMVLNYTSGTTSSPKGVVHCHRGIFTVALDSLVEWGVPKQSVYLWTLP 240
F+W+RP+SEWDP+VLNYTSGTTSSPKGVVH HR +F V LDSL++WGVPKQ VYLWTLP
Sbjct: 181 TFKWVRPISEWDPIVLNYTSGTTSSPKGVVHSHRSVFVVTLDSLIDWGVPKQPVYLWTLP 240
Query: 241 MFHANGWSFPWGIAAVGGTNVCIRKFDASLIFSLIRRHHVTHLCGAPVVLNMLTNSPDNR 300
MFHANGW + WGIAAVGGTN+C+R+FD +IF+LIRRH VTH+C AP+VLNML+NSP+
Sbjct: 241 MFHANGWGYTWGIAAVGGTNICLRRFDGEIIFNLIRRHRVTHMCAAPIVLNMLSNSPNAE 300
Query: 301 PLDRPVKILTAGAPPPAAVLLRIESLGFVVTHGYGLTESGGTIISCSWKSEWNRLTQTEQ 360
PL PV ++T GAPPPAAVLLR ESLGFV++HGYG+TE G ++SC+WK EWNRL TEQ
Sbjct: 301 PLPNPVHVMTGGAPPPAAVLLRTESLGFVISHGYGMTEMLGVVVSCAWKREWNRLPATEQ 360
Query: 361 ARLKARQGVRMLAVEAADVLDPKTRKSVKRDGASIGEIVVRGGSIMLGYLKDPEATAKAM 420
ARLK+RQGVR A+ DV+DP + SVKRDG ++GEIV++G SIMLGYLK+ ATAK +
Sbjct: 361 ARLKSRQGVRTAAMMEVDVVDPNSGVSVKRDGLTMGEIVLKGSSIMLGYLKNSAATAKCI 420
Query: 421 TDDGWFFTGDVGVMHPDGYIEIKDRSKDVIISGGENLSSVEVESVLYMNPVVNEVAVVAR 480
DGWF+TGD+ VMHPDGY+EIKDRSKDVIISGGEN+SSVEVES LY +P V+E AVVA
Sbjct: 421 RADGWFYTGDMAVMHPDGYLEIKDRSKDVIISGGENVSSVEVESALYSHPAVDEAAVVAC 480
Query: 481 PDEFWGETPCAFVNLKKGLTRQPTEEEIIEFCKGKLPRFMVPKTVVFTTELPKTSTGKIL 540
PDE+WGETP AFV LKKG+ +PTE+EI+E+C+ KL FMVPK VVF +LPKTSTGKI
Sbjct: 481 PDEYWGETPFAFVTLKKGMRVRPTEKEILEYCREKLAHFMVPKVVVFRDKLPKTSTGKIQ 540
Query: 541 KSVLREMAKSMGPTPKS 556
K L+E+ K+MG + S
Sbjct: 541 KFTLKEIVKTMGYSSSS 553
BLAST of Cla97C01G008675 vs. ExPASy Swiss-Prot
Match:
Q9FFE6 (Probable acyl-activating enzyme 5, peroxisomal OS=Arabidopsis thaliana OX=3702 GN=AAE5 PE=1 SV=1)
HSP 1 Score: 759.6 bits (1960), Expect = 2.5e-218
Identity = 368/558 (65.95%), Postives = 456/558 (81.72%), Query Frame = 0
Query: 1 MENLKPRPANSSPLTPIGFIERTALIYGDSPSIIYNS-TTYTWSETHRRCLQLASSLSSI 60
ME +KP ANS PLTPIGF+ER A +YGD SI+Y S T YTW ET+ RCL++ASSLSSI
Sbjct: 1 MEQMKPCAANSPPLTPIGFLERAATVYGDCTSIVYGSNTVYTWRETNLRCLRVASSLSSI 60
Query: 61 GIQRGHVVSVIAPNTPAMYELHFAVPMVGAVLNTINLRLDPRTISVLLRHSETKLIFVDQ 120
GI R VVSV++PNTPAMYEL FAVPM GA+LN IN RLD RT+SVLLRH +KL+FVD
Sbjct: 61 GIGRSDVVSVLSPNTPAMYELQFAVPMSGAILNNINTRLDARTVSVLLRHCGSKLLFVDV 120
Query: 121 ASCALISDALALFPSETKRPLLVLIADDTAVQDSSLSSSVLIRNDFVDSYEEMVWKGDSN 180
S L +A+++ T P+LV IAD ++ + V R F +Y++++ +GD +
Sbjct: 121 FSVDLAVEAISMM--TTDPPILVFIADK---EEEGGDADVADRTKFSYTYDDLIHRGDLD 180
Query: 181 FEWIRPVSEWDPMVLNYTSGTTSSPKGVVHCHRGIFTVALDSLVEWGVPKQSVYLWTLPM 240
F+WIRP SEWDP+VLNYTSGTTS+PKGVVHCHRGIF +++DSL++W VPK VYLWTLP+
Sbjct: 181 FKWIRPESEWDPVVLNYTSGTTSAPKGVVHCHRGIFVMSIDSLIDWTVPKNPVYLWTLPI 240
Query: 241 FHANGWSFPWGIAAVGGTNVCIRKFDASLIFSLIRRHHVTHLCGAPVVLNMLTNSPDNRP 300
FHANGWS+PWGIAAVGGTNVC+RKFDA LI+ LIR H VTH+CGAPVVLNML+ + + +P
Sbjct: 241 FHANGWSYPWGIAAVGGTNVCLRKFDAPLIYRLIRDHGVTHMCGAPVVLNMLSATNEFQP 300
Query: 301 LDRPVKILTAGAPPPAAVLLRIESLGFVVTHGYGLTESGGTIISCSWKSEWNRLTQTEQA 360
L+RPV ILTAGAPPPAAVLLR ES+GFV++HGYGLTE+ G +SC+WK +WNRL +++A
Sbjct: 301 LNRPVNILTAGAPPPAAVLLRAESIGFVISHGYGLTETAGLNVSCAWKPQWNRLPASDRA 360
Query: 361 RLKARQGVRMLAVEAADVLDPKTRKSVKRDGASIGEIVVRGGSIMLGYLKDPEATAKAMT 420
RLKARQGVR + DV+DP++ +SV+R+G ++GEIV+RG SIMLGYLKDP T KA+
Sbjct: 361 RLKARQGVRTVGFTEIDVVDPESGRSVERNGETVGEIVMRGSSIMLGYLKDPVGTEKAL- 420
Query: 421 DDGWFFTGDVGVMHPDGYIEIKDRSKDVIISGGENLSSVEVESVLYMNPVVNEVAVVARP 480
+GWF+TGDVGV+H DGY+EIKDRSKD+II+GGEN+SSVEVE+VLY NP VNEVAVVARP
Sbjct: 421 KNGWFYTGDVGVIHSDGYLEIKDRSKDIIITGGENVSSVEVETVLYTNPAVNEVAVVARP 480
Query: 481 DEFWGETPCAFVNLKKGLTRQPTEEEIIEFCKGKLPRFMVPKTVVFTTELPKTSTGKILK 540
D FWGETPCAFV+LK GLT++PTE E+IE+C+ K+P++MVPKTV F ELPKTSTGK++K
Sbjct: 481 DVFWGETPCAFVSLKSGLTQRPTEVEMIEYCRKKMPKYMVPKTVSFVDELPKTSTGKVMK 540
Query: 541 SVLREMAKSMGPTPKSRL 558
VLRE+AK MG T SR+
Sbjct: 541 FVLREIAKKMGTTRLSRM 552
BLAST of Cla97C01G008675 vs. ExPASy Swiss-Prot
Match:
Q9FFE9 (Probable acyl-activating enzyme 6 OS=Arabidopsis thaliana OX=3702 GN=AAE6 PE=2 SV=1)
HSP 1 Score: 740.0 bits (1909), Expect = 2.0e-212
Identity = 355/551 (64.43%), Postives = 448/551 (81.31%), Query Frame = 0
Query: 1 MENLKPRPANSSPLTPIGFIERTALIYGDSPSIIYNS-TTYTWSETHRRCLQLASSLSSI 60
ME +KP ANS PLTPIGF+ER A +YGD SI+Y S T YTW ET+ RCL++ASSLSSI
Sbjct: 1 MEEMKPCAANSPPLTPIGFLERAATVYGDCTSIVYGSNTVYTWRETNLRCLRVASSLSSI 60
Query: 61 GIQRGHVVSVIAPNTPAMYELHFAVPMVGAVLNTINLRLDPRTISVLLRHSETKLIFVDQ 120
GI R VVSV++PNTPAMYEL FAVPM GA+LN IN RLD RT+SVLLRH E+KL+FVD
Sbjct: 61 GIGRSDVVSVLSPNTPAMYELQFAVPMSGAILNNINTRLDARTVSVLLRHCESKLLFVDV 120
Query: 121 ASCALISDALALFPSETKRPLLVLIADDTAVQDSSLSSSVLIRNDFVDSYEEMVWKGDSN 180
S L +A+++ T P+LV+IAD ++ + V + F +Y++++ +GD
Sbjct: 121 FSVDLAVEAVSMM--TTDPPILVVIADK---EEEGGVADVADLSKFSYTYDDLIERGDPG 180
Query: 181 FEWIRPVSEWDPMVLNYTSGTTSSPKGVVHCHRGIFTVALDSLVEWGVPKQSVYLWTLPM 240
F+WIRP SEWDP+VLNYTSGTTS+PKGVVHCHRGIF +++DSL++W VPK VYLWTLP+
Sbjct: 181 FKWIRPESEWDPVVLNYTSGTTSAPKGVVHCHRGIFVMSVDSLIDWAVPKNPVYLWTLPI 240
Query: 241 FHANGWSFPWGIAAVGGTNVCIRKFDASLIFSLIRRHHVTHLCGAPVVLNMLTNSPDNRP 300
FH+NGW+ PWGIAAVGGTNVC+RKFDA LI+ LIR H VTH+CGAPVVLNML+ + +++P
Sbjct: 241 FHSNGWTNPWGIAAVGGTNVCLRKFDAPLIYRLIRDHGVTHMCGAPVVLNMLSATQESQP 300
Query: 301 LDRPVKILTAGAPPPAAVLLRIESLGFVVTHGYGLTESGGTIISCSWKSEWNRLTQTEQA 360
L+ PV ILTAG+PPPA VLLR ES+GFV++HGYGLTE+ G I+SC+WK +WN L +++A
Sbjct: 301 LNHPVNILTAGSPPPATVLLRAESIGFVISHGYGLTETAGVIVSCAWKPKWNHLPASDRA 360
Query: 361 RLKARQGVRMLAVEAADVLDPKTRKSVKRDGASIGEIVVRGGSIMLGYLKDPEATAKAMT 420
RLKARQGVR + DV+DP++ SV+R+G ++GEIV+RG S+MLGYLKDP T KA+
Sbjct: 361 RLKARQGVRTVGFTEIDVVDPESGLSVERNGETVGEIVMRGSSVMLGYLKDPVGTEKAL- 420
Query: 421 DDGWFFTGDVGVMHPDGYIEIKDRSKDVIISGGENLSSVEVESVLYMNPVVNEVAVVARP 480
+GWF+TGDVGV+H DGY+EIKDRSKD+II+GGEN+SSVEVE+VLY P VNEVAVVARP
Sbjct: 421 KNGWFYTGDVGVIHSDGYLEIKDRSKDIIITGGENVSSVEVETVLYTIPAVNEVAVVARP 480
Query: 481 DEFWGETPCAFVNLKKGLTRQPTEEEIIEFCKGKLPRFMVPKTVVFTTELPKTSTGKILK 540
DEFWGETPCAFV+LK G + +PTEEE++E+C+ K+P++MVPKTV F ELPK+STGK+ K
Sbjct: 481 DEFWGETPCAFVSLKNGFSGKPTEEELMEYCRKKMPKYMVPKTVSFMDELPKSSTGKVTK 540
Query: 541 SVLREMAKSMG 551
VLR++AK MG
Sbjct: 541 FVLRDIAKKMG 545
BLAST of Cla97C01G008675 vs. ExPASy Swiss-Prot
Match:
Q9LQS1 (Probable acyl-activating enzyme 8 OS=Arabidopsis thaliana OX=3702 GN=AAE8 PE=2 SV=1)
HSP 1 Score: 714.9 bits (1844), Expect = 7.0e-205
Identity = 350/551 (63.52%), Postives = 437/551 (79.31%), Query Frame = 0
Query: 1 MENLKPRPANSSPLTPIGFIERTALIYGDSPSIIY-NSTTYTWSETHRRCLQLASSLSSI 60
ME+LKP ANS PLT +GF+ER A +YGD SI+Y NST YTW ET+ RCL +AS+LSSI
Sbjct: 1 MEDLKPSAANSLPLTLLGFLERAATVYGDCTSIVYGNSTVYTWRETNHRCLCVASALSSI 60
Query: 61 GIQRGHVVSVIAPNTPAMYELHFAVPMVGAVLNTINLRLDPRTISVLLRHSETKLIFVDQ 120
GI R VVSV++ NTP MYEL F+VPM GA+LN IN RLD RT+SVLLRH E+KL+FVD
Sbjct: 61 GIGRSDVVSVLSANTPEMYELQFSVPMSGAILNNINTRLDARTVSVLLRHCESKLLFVDF 120
Query: 121 ASCALISDALALFPSETKRPLLVLIADDTAVQDSSLSSSVLIRNDFVDSYEEMVWKGDSN 180
L +A+ + P+LVLIA++ ++ + V R+ F Y +++ +G+ +
Sbjct: 121 FYSDLAVEAITML---LNPPILVLIANE---EEEEGGAEVTERSKFCYLYSDLITRGNPD 180
Query: 181 FEWIRPVSEWDPMVLNYTSGTTSSPKGVVHCHRGIFTVALDSLVEWGVPKQSVYLWTLPM 240
F+WIRP SEWDP+V+NYTSGTTSSPKGVVHCHRGIF + LDSL +W VPK VYLWTLP+
Sbjct: 181 FKWIRPGSEWDPIVVNYTSGTTSSPKGVVHCHRGIFVMTLDSLTDWAVPKTPVYLWTLPI 240
Query: 241 FHANGWSFPWGIAAVGGTNVCIRKFDASLIFSLIRRHHVTHLCGAPVVLNMLTNSPD-NR 300
FHANGW++PWGIAAVGGTNVC+RK A I+ LIR H VTH+ GAP+VL +L+ S + ++
Sbjct: 241 FHANGWTYPWGIAAVGGTNVCVRKLHAPSIYHLIRDHGVTHMYGAPIVLQILSASQESDQ 300
Query: 301 PLDRPVKILTAGAPPPAAVLLRIESLGFVVTHGYGLTESGGTIISCSWKSEWNRLTQTEQ 360
PL PV LTAG+ PPA VLLR ESLGF+V+HGYGLTE+ G I+SC+WK WNRL ++Q
Sbjct: 301 PLKSPVNFLTAGSSPPATVLLRAESLGFIVSHGYGLTETAGVIVSCAWKPNWNRLPASDQ 360
Query: 361 ARLKARQGVRMLAVEAADVLDPKTRKSVKRDGASIGEIVVRGGSIMLGYLKDPEATAKAM 420
A+LK+RQGVR + DV+DP++ +SV+RDG ++GEIV+RG SIMLGYLK+P T +
Sbjct: 361 AQLKSRQGVRTVGFSEIDVVDPESGRSVERDGETVGEIVLRGSSIMLGYLKNPIGTQNSF 420
Query: 421 TDDGWFFTGDVGVMHPDGYIEIKDRSKDVIISGGENLSSVEVESVLYMNPVVNEVAVVAR 480
+GWFFTGD+GV+H DGY+EIKDRSKDVIISGGEN+SSVEVE+VLY NP VNE AVVAR
Sbjct: 421 -KNGWFFTGDLGVIHGDGYLEIKDRSKDVIISGGENVSSVEVEAVLYTNPAVNEAAVVAR 480
Query: 481 PDEFWGETPCAFVNLKKGLTRQPTEEEIIEFCKGKLPRFMVPKTVVFTTELPKTSTGKIL 540
PDEFWGETPCAFV+LK GLTR+PT++EIIE+CK K+PR+M PKTV F ELPKTSTGKI+
Sbjct: 481 PDEFWGETPCAFVSLKPGLTRKPTDKEIIEYCKYKMPRYMAPKTVSFLEELPKTSTGKII 540
Query: 541 KSVLREMAKSM 550
KS+L+E+AK+M
Sbjct: 541 KSLLKEIAKNM 544
BLAST of Cla97C01G008675 vs. ExPASy TrEMBL
Match:
A0A5D3D623 (Putative acyl-activating enzyme 5, peroxisomal OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold418G00690 PE=4 SV=1)
HSP 1 Score: 997.7 bits (2578), Expect = 2.0e-287
Identity = 486/557 (87.25%), Postives = 521/557 (93.54%), Query Frame = 0
Query: 1 MENLKPRPANSSPLTPIGFIERTALIYGDSPSIIYNSTTYTWSETHRRCLQLASSLSSIG 60
ME+LKP PANS PLTPIGFI+R A IYGDS SIIYNSTTYTWSETHRRCLQLASS+SS G
Sbjct: 1 MEDLKPTPANSFPLTPIGFIQRAATIYGDSTSIIYNSTTYTWSETHRRCLQLASSISSFG 60
Query: 61 IQRGHVVSVIAPNTPAMYELHFAVPMVGAVLNTINLRLDPRTISVLLRHSETKLIFVDQA 120
IQRGHVVSVIAPNTPAMYELHFAVPMVGA+LNTINLRLD RTIS+LLRHSE+KLIFVDQA
Sbjct: 61 IQRGHVVSVIAPNTPAMYELHFAVPMVGAILNTINLRLDTRTISILLRHSESKLIFVDQA 120
Query: 121 SCALISDALALFPSETKRPLLVLIADDTAVQDSSLSSSVLIRNDFVDSYEEMVWKGDSNF 180
SCALI +ALALFP E KRPLLVLIADD A QDSSL S V IR++FVDSYE+MV +GDS F
Sbjct: 121 SCALIFEALALFPPEMKRPLLVLIADDAAEQDSSLPSFVSIRDNFVDSYEDMVKRGDSKF 180
Query: 181 EWIRPVSEWDPMVLNYTSGTTSSPKGVVHCHRGIFTVALDSLVEWGVPKQSVYLWTLPMF 240
+WIRP+SEWDP+VLNYTSGTTSSPKGVVHCHRGIFTVALDSL+EWGVPKQSVYLWTLPMF
Sbjct: 181 QWIRPLSEWDPIVLNYTSGTTSSPKGVVHCHRGIFTVALDSLLEWGVPKQSVYLWTLPMF 240
Query: 241 HANGWSFPWGIAAVGGTNVCIRKFDASLIFSLIRRHHVTHLCGAPVVLNMLTNSPDNRPL 300
H NGWSFPWGIAAVGGTNVCIRKFDA LIFSLIRRHHVTHLCGAPVVLNMLTNSPDN PL
Sbjct: 241 HGNGWSFPWGIAAVGGTNVCIRKFDARLIFSLIRRHHVTHLCGAPVVLNMLTNSPDNLPL 300
Query: 301 DRPVKILTAGAPPPAAVLLRIESLGFVVTHGYGLTESGGTIISCSWKSEWNRLTQTEQAR 360
DRPVKILTAGAPPPA++LLR ESLGFVVTHGYGLTES GTII CSWKSEWN+L EQAR
Sbjct: 301 DRPVKILTAGAPPPASILLRTESLGFVVTHGYGLTESAGTIICCSWKSEWNQLPAIEQAR 360
Query: 361 LKARQGVRMLAVEAADVLDPKTRKSVKRDGASIGEIVVRGGSIMLGYLKDPEATAKAMTD 420
LKARQGVRMLAVEA DVLDP+TRKSVKRDGASIGEIVVRG S+ LGYLKDPE TAKAM++
Sbjct: 361 LKARQGVRMLAVEAVDVLDPETRKSVKRDGASIGEIVVRGASVTLGYLKDPEGTAKAMSE 420
Query: 421 DGWFFTGDVGVMHPDGYIEIKDRSKDVIISGGENLSSVEVESVLYMNPVVNEVAVVARPD 480
DGWFFTGDVGVMHPDGY+EIKDRSKDVIISGGENLSSVEVESVLYMNP+VNE AVVARPD
Sbjct: 421 DGWFFTGDVGVMHPDGYLEIKDRSKDVIISGGENLSSVEVESVLYMNPMVNEAAVVARPD 480
Query: 481 EFWGETPCAFVNLKKGLTRQPTEEEIIEFCKGKLPRFMVPKTVVFTTELPKTSTGKILKS 540
EFWGETPCAF++LK+GL+R+PTEEEIIE+CKGKLPRFMVPKTV+F TE+PKT+TGKI K
Sbjct: 481 EFWGETPCAFISLKEGLSRKPTEEEIIEYCKGKLPRFMVPKTVIFMTEMPKTATGKIQKF 540
Query: 541 VLREMAKSMGPTPKSRL 558
VLR+ AKS+GP PKSRL
Sbjct: 541 VLRQKAKSLGPAPKSRL 557
BLAST of Cla97C01G008675 vs. ExPASy TrEMBL
Match:
A0A1S3CIL0 (probable acyl-activating enzyme 5, peroxisomal OS=Cucumis melo OX=3656 GN=LOC103501381 PE=4 SV=1)
HSP 1 Score: 997.7 bits (2578), Expect = 2.0e-287
Identity = 486/557 (87.25%), Postives = 521/557 (93.54%), Query Frame = 0
Query: 1 MENLKPRPANSSPLTPIGFIERTALIYGDSPSIIYNSTTYTWSETHRRCLQLASSLSSIG 60
ME+LKP PANS PLTPIGFI+R A IYGDS SIIYNSTTYTWSETHRRCLQLASS+SS G
Sbjct: 1 MEDLKPTPANSFPLTPIGFIQRAATIYGDSTSIIYNSTTYTWSETHRRCLQLASSISSFG 60
Query: 61 IQRGHVVSVIAPNTPAMYELHFAVPMVGAVLNTINLRLDPRTISVLLRHSETKLIFVDQA 120
IQRGHVVSVIAPNTPAMYELHFAVPMVGA+LNTINLRLD RTIS+LLRHSE+KLIFVDQA
Sbjct: 61 IQRGHVVSVIAPNTPAMYELHFAVPMVGAILNTINLRLDTRTISILLRHSESKLIFVDQA 120
Query: 121 SCALISDALALFPSETKRPLLVLIADDTAVQDSSLSSSVLIRNDFVDSYEEMVWKGDSNF 180
SCALI +ALALFP E KRPLLVLIADD A QDSSL S V IR++FVDSYE+MV +GDS F
Sbjct: 121 SCALIFEALALFPPEMKRPLLVLIADDAAEQDSSLPSFVSIRDNFVDSYEDMVKRGDSKF 180
Query: 181 EWIRPVSEWDPMVLNYTSGTTSSPKGVVHCHRGIFTVALDSLVEWGVPKQSVYLWTLPMF 240
+WIRP+SEWDP+VLNYTSGTTSSPKGVVHCHRGIFTVALDSL+EWGVPKQSVYLWTLPMF
Sbjct: 181 QWIRPLSEWDPIVLNYTSGTTSSPKGVVHCHRGIFTVALDSLLEWGVPKQSVYLWTLPMF 240
Query: 241 HANGWSFPWGIAAVGGTNVCIRKFDASLIFSLIRRHHVTHLCGAPVVLNMLTNSPDNRPL 300
H NGWSFPWGIAAVGGTNVCIRKFDA LIFSLIRRHHVTHLCGAPVVLNMLTNSPDN PL
Sbjct: 241 HGNGWSFPWGIAAVGGTNVCIRKFDARLIFSLIRRHHVTHLCGAPVVLNMLTNSPDNLPL 300
Query: 301 DRPVKILTAGAPPPAAVLLRIESLGFVVTHGYGLTESGGTIISCSWKSEWNRLTQTEQAR 360
DRPVKILTAGAPPPA++LLR ESLGFVVTHGYGLTES GTII CSWKSEWN+L EQAR
Sbjct: 301 DRPVKILTAGAPPPASILLRTESLGFVVTHGYGLTESAGTIICCSWKSEWNQLPAIEQAR 360
Query: 361 LKARQGVRMLAVEAADVLDPKTRKSVKRDGASIGEIVVRGGSIMLGYLKDPEATAKAMTD 420
LKARQGVRMLAVEA DVLDP+TRKSVKRDGASIGEIVVRG S+ LGYLKDPE TAKAM++
Sbjct: 361 LKARQGVRMLAVEAVDVLDPETRKSVKRDGASIGEIVVRGASVTLGYLKDPEGTAKAMSE 420
Query: 421 DGWFFTGDVGVMHPDGYIEIKDRSKDVIISGGENLSSVEVESVLYMNPVVNEVAVVARPD 480
DGWFFTGDVGVMHPDGY+EIKDRSKDVIISGGENLSSVEVESVLYMNP+VNE AVVARPD
Sbjct: 421 DGWFFTGDVGVMHPDGYLEIKDRSKDVIISGGENLSSVEVESVLYMNPMVNEAAVVARPD 480
Query: 481 EFWGETPCAFVNLKKGLTRQPTEEEIIEFCKGKLPRFMVPKTVVFTTELPKTSTGKILKS 540
EFWGETPCAF++LK+GL+R+PTEEEIIE+CKGKLPRFMVPKTV+F TE+PKT+TGKI K
Sbjct: 481 EFWGETPCAFISLKEGLSRKPTEEEIIEYCKGKLPRFMVPKTVIFMTEMPKTATGKIQKF 540
Query: 541 VLREMAKSMGPTPKSRL 558
VLR+ AKS+GP PKSRL
Sbjct: 541 VLRQKAKSLGPAPKSRL 557
BLAST of Cla97C01G008675 vs. ExPASy TrEMBL
Match:
A0A0A0KMT3 (AMP dependent ligase OS=Cucumis sativus OX=3659 GN=Csa_5G318390 PE=4 SV=1)
HSP 1 Score: 983.8 bits (2542), Expect = 3.0e-283
Identity = 479/557 (86.00%), Postives = 517/557 (92.82%), Query Frame = 0
Query: 1 MENLKPRPANSSPLTPIGFIERTALIYGDSPSIIYNSTTYTWSETHRRCLQLASSLSSIG 60
ME+LKPRPANS PLTPIGFIER A+IYGDS SIIYNSTTYTWSETHRRCLQLASS+SS G
Sbjct: 1 MEDLKPRPANSFPLTPIGFIERAAIIYGDSTSIIYNSTTYTWSETHRRCLQLASSISSFG 60
Query: 61 IQRGHVVSVIAPNTPAMYELHFAVPMVGAVLNTINLRLDPRTISVLLRHSETKLIFVDQA 120
IQRGHVVSV APNTPAMYELHFAVPM GA+LNTINLRLD RTIS+LLRHSE+KLIFVDQ
Sbjct: 61 IQRGHVVSVFAPNTPAMYELHFAVPMAGAILNTINLRLDTRTISILLRHSESKLIFVDQP 120
Query: 121 SCALISDALALFPSETKRPLLVLIADDTAVQDSSLSSSVLIRNDFVDSYEEMVWKGDSNF 180
SCALI DALALFP + KRPLLVLI DDTA QDSSL S V IR++FVDSYE MV GDS F
Sbjct: 121 SCALIFDALALFPPDMKRPLLVLITDDTAEQDSSLPSVVSIRDNFVDSYENMVKSGDSKF 180
Query: 181 EWIRPVSEWDPMVLNYTSGTTSSPKGVVHCHRGIFTVALDSLVEWGVPKQSVYLWTLPMF 240
+WIRP+SEWDP+VLNYTSGTTS+PKGVV+CHRG+FTVALDS++EWGVPKQSVYLWTLPMF
Sbjct: 181 QWIRPLSEWDPIVLNYTSGTTSAPKGVVNCHRGVFTVALDSILEWGVPKQSVYLWTLPMF 240
Query: 241 HANGWSFPWGIAAVGGTNVCIRKFDASLIFSLIRRHHVTHLCGAPVVLNMLTNSPDNRPL 300
H NGWSFPWGIAAVGGTNVCIRKFD+ LIFSLIRRHHVTHLCGAPVVLNMLTNSPDN PL
Sbjct: 241 HGNGWSFPWGIAAVGGTNVCIRKFDSRLIFSLIRRHHVTHLCGAPVVLNMLTNSPDNLPL 300
Query: 301 DRPVKILTAGAPPPAAVLLRIESLGFVVTHGYGLTESGGTIISCSWKSEWNRLTQTEQAR 360
DRPVKILTAGAPPPA+VLLR ESLGFVVTHGYGLTES GTII CSWKSEWN+L EQAR
Sbjct: 301 DRPVKILTAGAPPPASVLLRTESLGFVVTHGYGLTESAGTIICCSWKSEWNQLPAIEQAR 360
Query: 361 LKARQGVRMLAVEAADVLDPKTRKSVKRDGASIGEIVVRGGSIMLGYLKDPEATAKAMTD 420
LKARQGVRMLAVEA DVLDP+TRKSVKRDGASIGEIVVRG S+MLGYLKD EAT+KAM++
Sbjct: 361 LKARQGVRMLAVEAVDVLDPETRKSVKRDGASIGEIVVRGPSLMLGYLKDTEATSKAMSE 420
Query: 421 DGWFFTGDVGVMHPDGYIEIKDRSKDVIISGGENLSSVEVESVLYMNPVVNEVAVVARPD 480
DGWFFTGDVGVMHPDGYIEIKDRSKDVIISGGENLSSVEVESVLYMNP+VNE AVVARPD
Sbjct: 421 DGWFFTGDVGVMHPDGYIEIKDRSKDVIISGGENLSSVEVESVLYMNPMVNEAAVVARPD 480
Query: 481 EFWGETPCAFVNLKKGLTRQPTEEEIIEFCKGKLPRFMVPKTVVFTTELPKTSTGKILKS 540
EFWGETPCAF++LK+GL+R+PTEEEIIE+CKGKLP+FMVPKTV+F TE+PKT+TGK K
Sbjct: 481 EFWGETPCAFISLKEGLSRKPTEEEIIEYCKGKLPKFMVPKTVIFMTEMPKTATGKFQKF 540
Query: 541 VLREMAKSMGPTPKSRL 558
VLR+ AKS+GP KSRL
Sbjct: 541 VLRQKAKSLGPPQKSRL 557
BLAST of Cla97C01G008675 vs. ExPASy TrEMBL
Match:
A0A6J1FL76 (probable acyl-activating enzyme 5, peroxisomal OS=Cucurbita moschata OX=3662 GN=LOC111445243 PE=4 SV=1)
HSP 1 Score: 961.8 bits (2485), Expect = 1.2e-276
Identity = 471/557 (84.56%), Postives = 505/557 (90.66%), Query Frame = 0
Query: 1 MENLKPRPANSSPLTPIGFIERTALIYGDSPSIIYNSTTYTWSETHRRCLQLASSLSSIG 60
ME+LKP PANSSPLTPIGF+ER A++YGD PSIIYNSTTYTW+ETHRRCLQLASSL SIG
Sbjct: 1 MEDLKPNPANSSPLTPIGFLERAAVVYGDCPSIIYNSTTYTWTETHRRCLQLASSLVSIG 60
Query: 61 IQRGHVVSVIAPNTPAMYELHFAVPMVGAVLNTINLRLDPRTISVLLRHSETKLIFVDQA 120
IQRG VVSV++PNT AMYELHFAVPM GAVLNTINLRLD RTISV+LRH ET LIFVDQA
Sbjct: 61 IQRGQVVSVVSPNTAAMYELHFAVPMAGAVLNTINLRLDARTISVILRHCETNLIFVDQA 120
Query: 121 SCALISDALALFPSETKRPLLVLIADDTAVQDSSLSSSVLIRNDFVDSYEEMVWKGDSNF 180
SC LISDALALFP E KRP L+LIAD+ A +DSS +SSV R DFVDSYEEMV KGD F
Sbjct: 121 SCGLISDALALFPPEMKRPPLILIADNAAAEDSSSASSVWNRADFVDSYEEMVQKGDPLF 180
Query: 181 EWIRPVSEWDPMVLNYTSGTTSSPKGVVHCHRGIFTVALDSLVEWGVPKQSVYLWTLPMF 240
+WIRP SEWDP+VLNYTSGTTSSPKGVVHCHRGIFTV LDSL+EWGVPK+SVYLWTLPMF
Sbjct: 181 QWIRPASEWDPIVLNYTSGTTSSPKGVVHCHRGIFTVTLDSLIEWGVPKESVYLWTLPMF 240
Query: 241 HANGWSFPWGIAAVGGTNVCIRKFDASLIFSLIRRHHVTHLCGAPVVLNMLTNSPDNRPL 300
HANGWSFPWGIAAVGGTNVCIRKFDA+ IF+LI R+HVTHLCGAPVVLNMLTNSPDNRPL
Sbjct: 241 HANGWSFPWGIAAVGGTNVCIRKFDAAAIFALIHRYHVTHLCGAPVVLNMLTNSPDNRPL 300
Query: 301 DRPVKILTAGAPPPAAVLLRIESLGFVVTHGYGLTESGGTIISCSWKSEWNRLTQTEQAR 360
DRPVKILTAGAPPPAAVL R ESLGF V+HGYGLTE+GG +I CSWKS WNRL E+AR
Sbjct: 301 DRPVKILTAGAPPPAAVLFRTESLGFDVSHGYGLTETGGLVICCSWKSGWNRLPAAERAR 360
Query: 361 LKARQGVRMLAVEAADVLDPKTRKSVKRDGASIGEIVVRGGSIMLGYLKDPEATAKAMTD 420
LKARQGVR LA+ ADVLDP+T KSVKRDG SIGEIV+RG S+MLGYLKDPEATAKAMT
Sbjct: 361 LKARQGVRTLAMAVADVLDPETGKSVKRDGTSIGEIVLRGSSMMLGYLKDPEATAKAMTA 420
Query: 421 DGWFFTGDVGVMHPDGYIEIKDRSKDVIISGGENLSSVEVESVLYMNPVVNEVAVVARPD 480
+GWFFTGDVGVMHPDGY+EIKDRSKDVIISGGENLSSVEVESVLY NP VNE AVVARPD
Sbjct: 421 EGWFFTGDVGVMHPDGYLEIKDRSKDVIISGGENLSSVEVESVLYTNPAVNEAAVVARPD 480
Query: 481 EFWGETPCAFVNLKKGLTRQPTEEEIIEFCKGKLPRFMVPKTVVFTTELPKTSTGKILKS 540
EFWGETPCAFV+LK+GLTR+PTE EIIE+C+GKLPRFMVPKTVVFT ELPKTSTGKI K
Sbjct: 481 EFWGETPCAFVSLKEGLTRRPTEGEIIEYCRGKLPRFMVPKTVVFTAELPKTSTGKIQKF 540
Query: 541 VLREMAKSMGPTPKSRL 558
VLREMAKSMGP KSRL
Sbjct: 541 VLREMAKSMGPAGKSRL 557
BLAST of Cla97C01G008675 vs. ExPASy TrEMBL
Match:
A0A6J1JTQ2 (probable acyl-activating enzyme 5, peroxisomal OS=Cucurbita maxima OX=3661 GN=LOC111489657 PE=4 SV=1)
HSP 1 Score: 953.0 bits (2462), Expect = 5.6e-274
Identity = 471/561 (83.96%), Postives = 504/561 (89.84%), Query Frame = 0
Query: 1 MENLKPRPANSSPLTPIGFIERTALIYGDSPSIIYNSTTYTWSETHRRCLQLASSLSSIG 60
ME+LKP PANSSPLTPIGF+ER A++YGD PSIIYNSTTYTW+ETHRRCLQLASSL SIG
Sbjct: 1 MEDLKPNPANSSPLTPIGFLERAAVVYGDCPSIIYNSTTYTWTETHRRCLQLASSLVSIG 60
Query: 61 IQRGHVVSVIAPNTPAMYELHFAVPMVGAVLNTINLRLDPRTISVLLRHSETKLIFVDQA 120
IQRG VVSV++PNTPAMYELHFAVPM GAVLNTINLRLD RTISV+LRH ET LIFVDQA
Sbjct: 61 IQRGQVVSVVSPNTPAMYELHFAVPMAGAVLNTINLRLDSRTISVILRHCETNLIFVDQA 120
Query: 121 SCALISDALALFPSETKRPLLVLIADDTAVQDSSLS----SSVLIRNDFVDSYEEMVWKG 180
SC LISDALALFP E KRP L+LIAD+ A +DSS S SSV R DFVDSYEEMV KG
Sbjct: 121 SCGLISDALALFPPEMKRPPLILIADNAAAEDSSSSSSSASSVSNRADFVDSYEEMVQKG 180
Query: 181 DSNFEWIRPVSEWDPMVLNYTSGTTSSPKGVVHCHRGIFTVALDSLVEWGVPKQSVYLWT 240
D F+WIRPVSEWDP+VLNYTSGTTSSPKGVVHCHRGIFTV LDSL+EWGV KQSVYLWT
Sbjct: 181 DPLFQWIRPVSEWDPIVLNYTSGTTSSPKGVVHCHRGIFTVTLDSLIEWGVSKQSVYLWT 240
Query: 241 LPMFHANGWSFPWGIAAVGGTNVCIRKFDASLIFSLIRRHHVTHLCGAPVVLNMLTNSPD 300
LPMFHANGWSFPWGIAAVGGTNVCIRKFDA+ IF+LI R+HVTHLCGAPVVLNMLTNSPD
Sbjct: 241 LPMFHANGWSFPWGIAAVGGTNVCIRKFDAATIFALIHRYHVTHLCGAPVVLNMLTNSPD 300
Query: 301 NRPLDRPVKILTAGAPPPAAVLLRIESLGFVVTHGYGLTESGGTIISCSWKSEWNRLTQT 360
NRPLDRPVKILTAGAPPPAAVL R ESLGF V+HGYGLTE+GG +I CSWKS WNRL
Sbjct: 301 NRPLDRPVKILTAGAPPPAAVLFRTESLGFDVSHGYGLTETGGLVICCSWKSGWNRLPAA 360
Query: 361 EQARLKARQGVRMLAVEAADVLDPKTRKSVKRDGASIGEIVVRGGSIMLGYLKDPEATAK 420
E+ARLKARQGVR LA+ ADVL P+T KSVKRDG SIGEIV+RG S+MLGYLKDPEATAK
Sbjct: 361 ERARLKARQGVRTLAMAVADVLCPETGKSVKRDGTSIGEIVLRGSSMMLGYLKDPEATAK 420
Query: 421 AMTDDGWFFTGDVGVMHPDGYIEIKDRSKDVIISGGENLSSVEVESVLYMNPVVNEVAVV 480
AMT +GWFFTGDVGVMHPDGY+EIKDRSKDVIISGGENLSSVEVESVLY NP VNE AVV
Sbjct: 421 AMTAEGWFFTGDVGVMHPDGYLEIKDRSKDVIISGGENLSSVEVESVLYTNPAVNEAAVV 480
Query: 481 ARPDEFWGETPCAFVNLKKGLTRQPTEEEIIEFCKGKLPRFMVPKTVVFTTELPKTSTGK 540
ARPDEFWGETPCAFV+LK+GLTR+PTE EIIE+C+GKLPRFMVPKTVVF ELPKTSTGK
Sbjct: 481 ARPDEFWGETPCAFVSLKEGLTRRPTEGEIIEYCRGKLPRFMVPKTVVFMAELPKTSTGK 540
Query: 541 ILKSVLREMAKSMGPTPKSRL 558
I K VLREMAKSMGP +SRL
Sbjct: 541 IQKFVLREMAKSMGPAGRSRL 561
BLAST of Cla97C01G008675 vs. TAIR 10
Match:
AT5G16370.1 (acyl activating enzyme 5 )
HSP 1 Score: 759.6 bits (1960), Expect = 1.8e-219
Identity = 368/558 (65.95%), Postives = 456/558 (81.72%), Query Frame = 0
Query: 1 MENLKPRPANSSPLTPIGFIERTALIYGDSPSIIYNS-TTYTWSETHRRCLQLASSLSSI 60
ME +KP ANS PLTPIGF+ER A +YGD SI+Y S T YTW ET+ RCL++ASSLSSI
Sbjct: 1 MEQMKPCAANSPPLTPIGFLERAATVYGDCTSIVYGSNTVYTWRETNLRCLRVASSLSSI 60
Query: 61 GIQRGHVVSVIAPNTPAMYELHFAVPMVGAVLNTINLRLDPRTISVLLRHSETKLIFVDQ 120
GI R VVSV++PNTPAMYEL FAVPM GA+LN IN RLD RT+SVLLRH +KL+FVD
Sbjct: 61 GIGRSDVVSVLSPNTPAMYELQFAVPMSGAILNNINTRLDARTVSVLLRHCGSKLLFVDV 120
Query: 121 ASCALISDALALFPSETKRPLLVLIADDTAVQDSSLSSSVLIRNDFVDSYEEMVWKGDSN 180
S L +A+++ T P+LV IAD ++ + V R F +Y++++ +GD +
Sbjct: 121 FSVDLAVEAISMM--TTDPPILVFIADK---EEEGGDADVADRTKFSYTYDDLIHRGDLD 180
Query: 181 FEWIRPVSEWDPMVLNYTSGTTSSPKGVVHCHRGIFTVALDSLVEWGVPKQSVYLWTLPM 240
F+WIRP SEWDP+VLNYTSGTTS+PKGVVHCHRGIF +++DSL++W VPK VYLWTLP+
Sbjct: 181 FKWIRPESEWDPVVLNYTSGTTSAPKGVVHCHRGIFVMSIDSLIDWTVPKNPVYLWTLPI 240
Query: 241 FHANGWSFPWGIAAVGGTNVCIRKFDASLIFSLIRRHHVTHLCGAPVVLNMLTNSPDNRP 300
FHANGWS+PWGIAAVGGTNVC+RKFDA LI+ LIR H VTH+CGAPVVLNML+ + + +P
Sbjct: 241 FHANGWSYPWGIAAVGGTNVCLRKFDAPLIYRLIRDHGVTHMCGAPVVLNMLSATNEFQP 300
Query: 301 LDRPVKILTAGAPPPAAVLLRIESLGFVVTHGYGLTESGGTIISCSWKSEWNRLTQTEQA 360
L+RPV ILTAGAPPPAAVLLR ES+GFV++HGYGLTE+ G +SC+WK +WNRL +++A
Sbjct: 301 LNRPVNILTAGAPPPAAVLLRAESIGFVISHGYGLTETAGLNVSCAWKPQWNRLPASDRA 360
Query: 361 RLKARQGVRMLAVEAADVLDPKTRKSVKRDGASIGEIVVRGGSIMLGYLKDPEATAKAMT 420
RLKARQGVR + DV+DP++ +SV+R+G ++GEIV+RG SIMLGYLKDP T KA+
Sbjct: 361 RLKARQGVRTVGFTEIDVVDPESGRSVERNGETVGEIVMRGSSIMLGYLKDPVGTEKAL- 420
Query: 421 DDGWFFTGDVGVMHPDGYIEIKDRSKDVIISGGENLSSVEVESVLYMNPVVNEVAVVARP 480
+GWF+TGDVGV+H DGY+EIKDRSKD+II+GGEN+SSVEVE+VLY NP VNEVAVVARP
Sbjct: 421 KNGWFYTGDVGVIHSDGYLEIKDRSKDIIITGGENVSSVEVETVLYTNPAVNEVAVVARP 480
Query: 481 DEFWGETPCAFVNLKKGLTRQPTEEEIIEFCKGKLPRFMVPKTVVFTTELPKTSTGKILK 540
D FWGETPCAFV+LK GLT++PTE E+IE+C+ K+P++MVPKTV F ELPKTSTGK++K
Sbjct: 481 DVFWGETPCAFVSLKSGLTQRPTEVEMIEYCRKKMPKYMVPKTVSFVDELPKTSTGKVMK 540
Query: 541 SVLREMAKSMGPTPKSRL 558
VLRE+AK MG T SR+
Sbjct: 541 FVLREIAKKMGTTRLSRM 552
BLAST of Cla97C01G008675 vs. TAIR 10
Match:
AT5G16340.1 (AMP-dependent synthetase and ligase family protein )
HSP 1 Score: 740.0 bits (1909), Expect = 1.4e-213
Identity = 355/551 (64.43%), Postives = 448/551 (81.31%), Query Frame = 0
Query: 1 MENLKPRPANSSPLTPIGFIERTALIYGDSPSIIYNS-TTYTWSETHRRCLQLASSLSSI 60
ME +KP ANS PLTPIGF+ER A +YGD SI+Y S T YTW ET+ RCL++ASSLSSI
Sbjct: 1 MEEMKPCAANSPPLTPIGFLERAATVYGDCTSIVYGSNTVYTWRETNLRCLRVASSLSSI 60
Query: 61 GIQRGHVVSVIAPNTPAMYELHFAVPMVGAVLNTINLRLDPRTISVLLRHSETKLIFVDQ 120
GI R VVSV++PNTPAMYEL FAVPM GA+LN IN RLD RT+SVLLRH E+KL+FVD
Sbjct: 61 GIGRSDVVSVLSPNTPAMYELQFAVPMSGAILNNINTRLDARTVSVLLRHCESKLLFVDV 120
Query: 121 ASCALISDALALFPSETKRPLLVLIADDTAVQDSSLSSSVLIRNDFVDSYEEMVWKGDSN 180
S L +A+++ T P+LV+IAD ++ + V + F +Y++++ +GD
Sbjct: 121 FSVDLAVEAVSMM--TTDPPILVVIADK---EEEGGVADVADLSKFSYTYDDLIERGDPG 180
Query: 181 FEWIRPVSEWDPMVLNYTSGTTSSPKGVVHCHRGIFTVALDSLVEWGVPKQSVYLWTLPM 240
F+WIRP SEWDP+VLNYTSGTTS+PKGVVHCHRGIF +++DSL++W VPK VYLWTLP+
Sbjct: 181 FKWIRPESEWDPVVLNYTSGTTSAPKGVVHCHRGIFVMSVDSLIDWAVPKNPVYLWTLPI 240
Query: 241 FHANGWSFPWGIAAVGGTNVCIRKFDASLIFSLIRRHHVTHLCGAPVVLNMLTNSPDNRP 300
FH+NGW+ PWGIAAVGGTNVC+RKFDA LI+ LIR H VTH+CGAPVVLNML+ + +++P
Sbjct: 241 FHSNGWTNPWGIAAVGGTNVCLRKFDAPLIYRLIRDHGVTHMCGAPVVLNMLSATQESQP 300
Query: 301 LDRPVKILTAGAPPPAAVLLRIESLGFVVTHGYGLTESGGTIISCSWKSEWNRLTQTEQA 360
L+ PV ILTAG+PPPA VLLR ES+GFV++HGYGLTE+ G I+SC+WK +WN L +++A
Sbjct: 301 LNHPVNILTAGSPPPATVLLRAESIGFVISHGYGLTETAGVIVSCAWKPKWNHLPASDRA 360
Query: 361 RLKARQGVRMLAVEAADVLDPKTRKSVKRDGASIGEIVVRGGSIMLGYLKDPEATAKAMT 420
RLKARQGVR + DV+DP++ SV+R+G ++GEIV+RG S+MLGYLKDP T KA+
Sbjct: 361 RLKARQGVRTVGFTEIDVVDPESGLSVERNGETVGEIVMRGSSVMLGYLKDPVGTEKAL- 420
Query: 421 DDGWFFTGDVGVMHPDGYIEIKDRSKDVIISGGENLSSVEVESVLYMNPVVNEVAVVARP 480
+GWF+TGDVGV+H DGY+EIKDRSKD+II+GGEN+SSVEVE+VLY P VNEVAVVARP
Sbjct: 421 KNGWFYTGDVGVIHSDGYLEIKDRSKDIIITGGENVSSVEVETVLYTIPAVNEVAVVARP 480
Query: 481 DEFWGETPCAFVNLKKGLTRQPTEEEIIEFCKGKLPRFMVPKTVVFTTELPKTSTGKILK 540
DEFWGETPCAFV+LK G + +PTEEE++E+C+ K+P++MVPKTV F ELPK+STGK+ K
Sbjct: 481 DEFWGETPCAFVSLKNGFSGKPTEEELMEYCRKKMPKYMVPKTVSFMDELPKSSTGKVTK 540
Query: 541 SVLREMAKSMG 551
VLR++AK MG
Sbjct: 541 FVLRDIAKKMG 545
BLAST of Cla97C01G008675 vs. TAIR 10
Match:
AT1G75960.1 (AMP-dependent synthetase and ligase family protein )
HSP 1 Score: 714.9 bits (1844), Expect = 5.0e-206
Identity = 350/551 (63.52%), Postives = 437/551 (79.31%), Query Frame = 0
Query: 1 MENLKPRPANSSPLTPIGFIERTALIYGDSPSIIY-NSTTYTWSETHRRCLQLASSLSSI 60
ME+LKP ANS PLT +GF+ER A +YGD SI+Y NST YTW ET+ RCL +AS+LSSI
Sbjct: 1 MEDLKPSAANSLPLTLLGFLERAATVYGDCTSIVYGNSTVYTWRETNHRCLCVASALSSI 60
Query: 61 GIQRGHVVSVIAPNTPAMYELHFAVPMVGAVLNTINLRLDPRTISVLLRHSETKLIFVDQ 120
GI R VVSV++ NTP MYEL F+VPM GA+LN IN RLD RT+SVLLRH E+KL+FVD
Sbjct: 61 GIGRSDVVSVLSANTPEMYELQFSVPMSGAILNNINTRLDARTVSVLLRHCESKLLFVDF 120
Query: 121 ASCALISDALALFPSETKRPLLVLIADDTAVQDSSLSSSVLIRNDFVDSYEEMVWKGDSN 180
L +A+ + P+LVLIA++ ++ + V R+ F Y +++ +G+ +
Sbjct: 121 FYSDLAVEAITML---LNPPILVLIANE---EEEEGGAEVTERSKFCYLYSDLITRGNPD 180
Query: 181 FEWIRPVSEWDPMVLNYTSGTTSSPKGVVHCHRGIFTVALDSLVEWGVPKQSVYLWTLPM 240
F+WIRP SEWDP+V+NYTSGTTSSPKGVVHCHRGIF + LDSL +W VPK VYLWTLP+
Sbjct: 181 FKWIRPGSEWDPIVVNYTSGTTSSPKGVVHCHRGIFVMTLDSLTDWAVPKTPVYLWTLPI 240
Query: 241 FHANGWSFPWGIAAVGGTNVCIRKFDASLIFSLIRRHHVTHLCGAPVVLNMLTNSPD-NR 300
FHANGW++PWGIAAVGGTNVC+RK A I+ LIR H VTH+ GAP+VL +L+ S + ++
Sbjct: 241 FHANGWTYPWGIAAVGGTNVCVRKLHAPSIYHLIRDHGVTHMYGAPIVLQILSASQESDQ 300
Query: 301 PLDRPVKILTAGAPPPAAVLLRIESLGFVVTHGYGLTESGGTIISCSWKSEWNRLTQTEQ 360
PL PV LTAG+ PPA VLLR ESLGF+V+HGYGLTE+ G I+SC+WK WNRL ++Q
Sbjct: 301 PLKSPVNFLTAGSSPPATVLLRAESLGFIVSHGYGLTETAGVIVSCAWKPNWNRLPASDQ 360
Query: 361 ARLKARQGVRMLAVEAADVLDPKTRKSVKRDGASIGEIVVRGGSIMLGYLKDPEATAKAM 420
A+LK+RQGVR + DV+DP++ +SV+RDG ++GEIV+RG SIMLGYLK+P T +
Sbjct: 361 AQLKSRQGVRTVGFSEIDVVDPESGRSVERDGETVGEIVLRGSSIMLGYLKNPIGTQNSF 420
Query: 421 TDDGWFFTGDVGVMHPDGYIEIKDRSKDVIISGGENLSSVEVESVLYMNPVVNEVAVVAR 480
+GWFFTGD+GV+H DGY+EIKDRSKDVIISGGEN+SSVEVE+VLY NP VNE AVVAR
Sbjct: 421 -KNGWFFTGDLGVIHGDGYLEIKDRSKDVIISGGENVSSVEVEAVLYTNPAVNEAAVVAR 480
Query: 481 PDEFWGETPCAFVNLKKGLTRQPTEEEIIEFCKGKLPRFMVPKTVVFTTELPKTSTGKIL 540
PDEFWGETPCAFV+LK GLTR+PT++EIIE+CK K+PR+M PKTV F ELPKTSTGKI+
Sbjct: 481 PDEFWGETPCAFVSLKPGLTRKPTDKEIIEYCKYKMPRYMAPKTVSFLEELPKTSTGKII 540
Query: 541 KSVLREMAKSM 550
KS+L+E+AK+M
Sbjct: 541 KSLLKEIAKNM 544
BLAST of Cla97C01G008675 vs. TAIR 10
Match:
AT1G21540.1 (AMP-dependent synthetase and ligase family protein )
HSP 1 Score: 704.9 bits (1818), Expect = 5.1e-203
Identity = 340/557 (61.04%), Postives = 434/557 (77.92%), Query Frame = 0
Query: 1 MENLKPRPANSSPLTPIGFIERTALIYGDSPSIIY-NSTTYTWSETHRRCLQLASSL--S 60
ME L P P+NS+PLT +GF++R A +YGD PSI++ +T +TWSETH RCL++AS+L S
Sbjct: 1 MELLLPHPSNSTPLTVLGFLDRAASVYGDCPSILHTTNTVHTWSETHNRCLRIASALTSS 60
Query: 61 SIGIQRGHVVSVIAPNTPAMYELHFAVPMVGAVLNTINLRLDPRTISVLLRHSETKLIFV 120
S+GI RG VVSV+ PN P++YEL FAVPM GA+LN IN RLD +SVLLRHSE+KL+FV
Sbjct: 61 SLGINRGQVVSVVGPNVPSVYELQFAVPMSGAILNNINPRLDAHALSVLLRHSESKLVFV 120
Query: 121 DQASCALISDALALFPSETKRPLLVLIADDTAVQDSSLSSSVLIRNDFVDSYEEMVWKGD 180
D S +++ +A++ F + ++P LVL+ DD +D SLS S +DF+D+Y+ ++ +GD
Sbjct: 121 DPNSISVVLEAVS-FMRQNEKPHLVLLDDDQ--EDGSLSPSA--ASDFLDTYQGVMERGD 180
Query: 181 SNFEWIRPVSEWDPMVLNYTSGTTSSPKGVVHCHRGIFTVALDSLVEWGVPKQSVYLWTL 240
S F+WIRP +EW PM+LNYTSGTTSSPKGVV HR IF + + SL++W P + VYLWTL
Sbjct: 181 SRFKWIRPQTEWQPMILNYTSGTTSSPKGVVLSHRAIFMLTVSSLLDWHFPNRPVYLWTL 240
Query: 241 PMFHANGWSFPWGIAAVGGTNVCIRKFDASLIFSLIRRHHVTHLCGAPVVLNMLTNSPDN 300
PMFHANGW + WG AAVG TNVC R+ DA I+ LI +HHVTH+C AP+VLNMLTN P
Sbjct: 241 PMFHANGWGYTWGTAAVGATNVCTRRVDAPTIYDLIDKHHVTHMCAAPMVLNMLTNYPSR 300
Query: 301 RPLDRPVKILTAGAPPPAAVLLRIESLGFVVTHGYGLTESGGTIISCSWKSEWNRLTQTE 360
+PL PV+++TAGAPPPAA++ R E+LGF V HGYGLTE+GG ++SC+WK+EW+ L E
Sbjct: 301 KPLKNPVQVMTAGAPPPAAIISRAETLGFNVGHGYGLTETGGPVVSCAWKAEWDHLDPLE 360
Query: 361 QARLKARQGVRMLAVEAADVLDPKTRKSVKRDGASIGEIVVRGGSIMLGYLKDPEATAKA 420
+ARLK+RQGVR + DV DP+T KSV+ DG S+GEIV++GGS+MLGY KDPE TA
Sbjct: 361 RARLKSRQGVRTIGFAEVDVRDPRTGKSVEHDGVSVGEIVLKGGSVMLGYYKDPEGTAAC 420
Query: 421 MTDDGWFFTGDVGVMHPDGYIEIKDRSKDVIISGGENLSSVEVESVLYMNPVVNEVAVVA 480
M +DGWF++GDVGV+H DGY+E+KDRSKDVII GGEN+SS EVE+VLY NPVV E AVVA
Sbjct: 421 MREDGWFYSGDVGVIHEDGYLEVKDRSKDVIICGGENISSAEVETVLYTNPVVKEAAVVA 480
Query: 481 RPDEFWGETPCAFVNLK-----KGLTRQPTEEEIIEFCKGKLPRFMVPKTVVFTTELPKT 540
+PD+ WGETPCAFV+LK GL TE EI EFCK +LP++MVP+ V+F ELPKT
Sbjct: 481 KPDKMWGETPCAFVSLKYDSNGNGLV---TEREIREFCKTRLPKYMVPRKVIFQEELPKT 540
Query: 541 STGKILKSVLREMAKSM 550
STGKI K +LR+MAKS+
Sbjct: 541 STGKIQKFLLRQMAKSL 549
BLAST of Cla97C01G008675 vs. TAIR 10
Match:
AT1G77240.1 (AMP-dependent synthetase and ligase family protein )
HSP 1 Score: 693.0 bits (1787), Expect = 2.0e-199
Identity = 329/551 (59.71%), Postives = 422/551 (76.59%), Query Frame = 0
Query: 1 MENLKPRPANSSPLTPIGFIERTALIYGDSPSIIYNSTTYTWSETHRRCLQLASSLS--S 60
ME L P +NS PLT +GF+ER A ++GDSPS+++ +T +TWSETH RCL++AS+LS S
Sbjct: 1 MELLLPHASNSCPLTVLGFLERAASVFGDSPSLLHTTTVHTWSETHSRCLRIASTLSSAS 60
Query: 61 IGIQRGHVVSVIAPNTPAMYELHFAVPMVGAVLNTINLRLDPRTISVLLRHSETKLIFVD 120
+GI RG VVSVI PN P++YEL FAVPM GAVLN IN RLD +SVLLRHSE+KL+FVD
Sbjct: 61 LGINRGQVVSVIGPNVPSVYELQFAVPMSGAVLNNINPRLDAHALSVLLRHSESKLVFVD 120
Query: 121 QASCALISDALALFPSETKRPLLVLIADDTAVQDSSLSSSVLIRNDFVDSYEEMVWKGDS 180
S +L+ +A++ P + +RP LV++ D + SS + DF+D+YE + +GD
Sbjct: 121 HHSSSLVLEAVSFLPKD-ERPRLVILNDGNDMPSSSSADM-----DFLDTYEGFMERGDL 180
Query: 181 NFEWIRPVSEWDPMVLNYTSGTTSSPKGVVHCHRGIFTVALDSLVEWGVPKQSVYLWTLP 240
F+W+RP SEW PMVLNYTSGTTSSPKGVVH HR +F ++SL++W +P + VYLWTLP
Sbjct: 181 RFKWVRPKSEWTPMVLNYTSGTTSSPKGVVHSHRSVFMSTINSLLDWSLPNRPVYLWTLP 240
Query: 241 MFHANGWSFPWGIAAVGGTNVCIRKFDASLIFSLIRRHHVTHLCGAPVVLNMLTNSPDNR 300
MFHANGWS+ W AAVG N+C+ + D IF+LI ++ VTH+C AP+VLNMLTN P +
Sbjct: 241 MFHANGWSYTWATAAVGARNICVTRVDVPTIFNLIDKYQVTHMCAAPMVLNMLTNHPAQK 300
Query: 301 PLDRPVKILTAGAPPPAAVLLRIESLGFVVTHGYGLTESGGTIISCSWKSEWNRLTQTEQ 360
PL PVK++TAGAPPPA V+ + E+LGF V+HGYG+TE+GG ++SC+ K EW+RL E+
Sbjct: 301 PLQSPVKVMTAGAPPPATVISKAEALGFDVSHGYGMTETGGLVVSCALKPEWDRLEPDER 360
Query: 361 ARLKARQGVRMLAVEAADVLDPKTRKSVKRDGASIGEIVVRGGSIMLGYLKDPEATAKAM 420
A+ K+RQG+R DV DP + KSVK DGA++GEIV RGGS+MLGY KDPE TA +M
Sbjct: 361 AKQKSRQGIRTAVFAEVDVRDPISGKSVKHDGATVGEIVFRGGSVMLGYYKDPEGTAASM 420
Query: 421 TDDGWFFTGDVGVMHPDGYIEIKDRSKDVIISGGENLSSVEVESVLYMNPVVNEVAVVAR 480
+DGWF+TGD+GVMHPDGY+E+KDRSKDV+I GGEN+SS E+E+VLY NP + E AVVA+
Sbjct: 421 REDGWFYTGDIGVMHPDGYLEVKDRSKDVVICGGENISSTELEAVLYTNPAIKEAAVVAK 480
Query: 481 PDEFWGETPCAFVNLKKGLTRQPTEEEIIEFCKGKLPRFMVPKTVVFTTELPKTSTGKIL 540
PD+ WGETPCAFV+LK TE EI EFCK KLP++MVP+ VVF ELPKTSTGKI
Sbjct: 481 PDKMWGETPCAFVSLKYH-DGSVTEREIREFCKTKLPKYMVPRNVVFLEELPKTSTGKIQ 540
Query: 541 KSVLREMAKSM 550
K +LR+MAKS+
Sbjct: 541 KFLLRQMAKSL 544
The following BLAST results are available for this feature:
Match Name | E-value | Identity | Description | |
XP_008463174.1 | 4.1e-287 | 87.25 | PREDICTED: probable acyl-activating enzyme 5, peroxisomal [Cucumis melo] >KAA004... | [more] |
XP_004148908.1 | 6.1e-283 | 86.00 | probable acyl-activating enzyme 5, peroxisomal [Cucumis sativus] >KGN50898.1 hyp... | [more] |
XP_022939278.1 | 2.5e-276 | 84.56 | probable acyl-activating enzyme 5, peroxisomal [Cucurbita moschata] >KAG6579057.... | [more] |
XP_022993747.1 | 1.2e-273 | 83.96 | probable acyl-activating enzyme 5, peroxisomal [Cucurbita maxima] | [more] |
XP_023550909.1 | 1.2e-273 | 83.78 | probable acyl-activating enzyme 5, peroxisomal [Cucurbita pepo subsp. pepo] | [more] |
Match Name | E-value | Identity | Description | |
M4IQQ5 | 5.0e-235 | 68.18 | 2-methylpropanoate--CoA ligase CCL4 OS=Humulus lupulus OX=3486 GN=CCL4 PE=1 SV=1 | [more] |
M4ISH2 | 1.3e-219 | 65.35 | Probable CoA ligase CCL11 OS=Humulus lupulus OX=3486 GN=CCL11 PE=2 SV=1 | [more] |
Q9FFE6 | 2.5e-218 | 65.95 | Probable acyl-activating enzyme 5, peroxisomal OS=Arabidopsis thaliana OX=3702 G... | [more] |
Q9FFE9 | 2.0e-212 | 64.43 | Probable acyl-activating enzyme 6 OS=Arabidopsis thaliana OX=3702 GN=AAE6 PE=2 S... | [more] |
Q9LQS1 | 7.0e-205 | 63.52 | Probable acyl-activating enzyme 8 OS=Arabidopsis thaliana OX=3702 GN=AAE8 PE=2 S... | [more] |
Match Name | E-value | Identity | Description | |
A0A5D3D623 | 2.0e-287 | 87.25 | Putative acyl-activating enzyme 5, peroxisomal OS=Cucumis melo var. makuwa OX=11... | [more] |
A0A1S3CIL0 | 2.0e-287 | 87.25 | probable acyl-activating enzyme 5, peroxisomal OS=Cucumis melo OX=3656 GN=LOC103... | [more] |
A0A0A0KMT3 | 3.0e-283 | 86.00 | AMP dependent ligase OS=Cucumis sativus OX=3659 GN=Csa_5G318390 PE=4 SV=1 | [more] |
A0A6J1FL76 | 1.2e-276 | 84.56 | probable acyl-activating enzyme 5, peroxisomal OS=Cucurbita moschata OX=3662 GN=... | [more] |
A0A6J1JTQ2 | 5.6e-274 | 83.96 | probable acyl-activating enzyme 5, peroxisomal OS=Cucurbita maxima OX=3661 GN=LO... | [more] |