Cla97C01G001520 (gene) Watermelon (97103) v2.5

Overview
NameCla97C01G001520
Typegene
OrganismCitrullus lanatus subsp. vulgaris cv. 97103 (Watermelon (97103) v2.5)
Descriptionmethionine gamma-lyase-like
LocationCla97Chr01: 1408133 .. 1410909 (-)
RNA-Seq ExpressionCla97C01G001520
SyntenyCla97C01G001520
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: polypeptideexonCDS
Hold the cursor over a type above to highlight its positions in the sequence below.
ATGATAACAGAAGTAAAAGCTCACAACACTTCTCTTCCATGTTGGAACAACAACAATGATAAGTTCAGTTACGACACGACATTGGCTCCATCATGGCCAATGAGAGAGGAGGATCCAGTGGCCGCTTTGGCAATGGCCCGCCCCGAGTTCGGAGAGCACGGTGGTGTGAACTTGTCGATCGAAAACTCAATAACATTCACGGTGATGGAGGCCGAAACGATGCGTAAGATGTTCGAAGGAGAGTTGGGGCCTGAACGGGACTTCTTCATCTATAGCCGCCACTTCCACCCGACCGTCATGGCCCTTAGCCGGCAGATGGCGGCGCTGGAAGGGACCGAGGCGGCATATTGCACGTCGAGTGGGATGGCTGCCATTTCATCCGTTTTGTTGCAGCTTGTGAACTCCGGCGGGCATATCGTGGCATCGAGAACGCTCTACGGCGGCACTCACGCGTTGCTGTCGCATTTTCTGCCGAGGGTCTGCAACATAACGACGACGTTTGTAGACGTGAAGGAGCATGAAATGGTTAGAGATGCCATGGTAGAGGGAAAGACGAAGGTATAATCTAACACCAACTTAAAATTTTATGTTCATTATTGAATTTTATAGATTTGGTTTTTACACCATCAATTTGATTTTATAATTTTATTAGCTTTCATTCAATTTATTTAATTTGGATTTAGTTTTGGTTTGAATTTAATACATAGTTCTAAATGTTTCAATATTAATTTTTTTATTAATTGTTTGGCTAACCGTGTGAGTTTGGCTATATTAAACTTTTTACAAGTAAATAAAAAAAAGTACTCTGTTATTTCATCCGATTCGCTTTAAAGTAATATGTAACATAAAACGAATAAATAAAATAAAATTTTAGAATGATTTGTGGAGTTTAACCAAACCCATAGGAACTAAGATTAGGCCATAAGAACTAAATTGAACTTTAAAAAGTGCATTTGATTAAGATTTTTTTTTTCTTTTTCAAATAATAATAATAATAATAATAAATAAATAAATAAATAAATAAACAAATAAAAACCTCTATTAACACCTATAATCACAACCGTCTCAAAAAAATACATTTTTTTCAAAAGTTATTCCATACTGACTATAAAACTGTGTTCAAAACTAATAGGGACTAAATTATATAATTCTCTGATCTTTTGTTCATATTCTTTTTAACACTTCCCTAATTTCTGGATCTCGACAATTAACACATAAAAGATTGATAATTAAACGTTAATTAGAAAGAAAAAATAAATTATTTTACTAGCGTTGGAATTCGAGTTCTTGCTTGGATAACATGTTAAATCAATTCAAAATTAATCTAGGTTTATAGGTTACAATGAATTAAATTTTTTACTCTTATTATAAACCCAGGTATTGTATTTCGAGACGGTGGCGAACCCGACACTGACGGTGGCGAATGTCCCCGCGCTCAGCCGGATGGCGCACAAGAAGGGGGTGACAGTGGTGGTCGATAATACGTTCTCGCCCATGATCATCTCACCGGTGAGGCTCGGTGCTGACATCGTGGTTCATAGCTGCTCCAAGTTTATCAGCGGCGGAGCAGACATTATTGCTGGTATGATGTCTTTCTAATTTAGTTTTCATTGTTAAGTGAGCCAATTAAAGGGATGTCTATATGTGCCCTTATCCATGTTGCTTAATCAAAGACTATCTGGGTTTTATATTTATGATTTCAAACCTTAATTTACGCAACACTAGTCATACTGAAGCCCATTATTTAATAGTCAATATTCATCCAAACAAGTTCTGAAAGTCTAGTCTTTGTTTTATATTATATATTTACTCTTGTCTATTTGATTATTAGTAAAGCTGATATTAAAAGATTTTAGTCTATCTAATGTAAACATGATTTTAATACGGTTGTTCTTATAACCTTAAAAAATTTGGCTACTATGTTGTTTAATGGATGGTATGTTTTATTTTGGCATATGGTTTAAAGAGGTTTGTTGAGTTGTCATTATATTGTGAAATGTTTTTAAATTATAGGTAATGTTAATTCAAATATATATATATATATATATTCAAACTCAGGATCACCAATTGTGTTAGATGGTGTGATTTATTCTTTGGTCTTTTTTAGGTGCTGTTTGTGGGTCAAAAAGTTTGGTGAATTCTATGATGGACCTTCACCAAGGCACACTGATGCTTCTTGGGCCAACAATGAATGCTAAAGTTGCATCTGAACTCTCTCAAAGAATCCCCCATTTAAGTCTTCGAATGAAAGAGCACTCTCACAGAGCTTTAGTTTTTGCTCAAAGAATGAAGAATCTCGGCCTCAAAGTTATATACCCTGGTCTCAACGATCACCCCGACCACCATCTTCTAAGCTCCATAGCCAACAAAGACTACGGTTATGGCGGAGTTTTGTGCATCGACATGGGGACCGAAGAGAGAGCCAATAGCCTCATGAATATTCTTCAAAACCATACACATTTTGGTCTCATGGCAGTTAGCTTGGGCTTCTTCGAGACCCTCATGTCATGCTCAGGTAGGGTTAAATCACTCTCAGTGATCAACTTAAAAACTTAAGCAAATGGGTTACGTTAAATTTAATTATCAATTCATTTTTCTTTCTTTTTTTTTTTTTTTCTTTTTGGACAGGTAGTAGCACGAGTAGCGAGTTGAACGGCGAGGAGAAGGAATTGGCTGGCATTTCTCCAGGATTGATTAGGATGTCTGTTGGATATTTTGGAACCTTAGAGCAGAAATGGAGCCAATTGGAGAAGGCACTGTTGAAGATGAAGATTTGA

mRNA sequence

ATGATAACAGAAGTAAAAGCTCACAACACTTCTCTTCCATGTTGGAACAACAACAATGATAAGTTCAGTTACGACACGACATTGGCTCCATCATGGCCAATGAGAGAGGAGGATCCAGTGGCCGCTTTGGCAATGGCCCGCCCCGAGTTCGGAGAGCACGGTGGTGTGAACTTGTCGATCGAAAACTCAATAACATTCACGGTGATGGAGGCCGAAACGATGCGTAAGATGTTCGAAGGAGAGTTGGGGCCTGAACGGGACTTCTTCATCTATAGCCGCCACTTCCACCCGACCGTCATGGCCCTTAGCCGGCAGATGGCGGCGCTGGAAGGGACCGAGGCGGCATATTGCACGTCGAGTGGGATGGCTGCCATTTCATCCGTTTTGTTGCAGCTTGTGAACTCCGGCGGGCATATCGTGGCATCGAGAACGCTCTACGGCGGCACTCACGCGTTGCTGTCGCATTTTCTGCCGAGGGTCTGCAACATAACGACGACGTTTGTAGACGTGAAGGAGCATGAAATGGTTAGAGATGCCATGGTAGAGGGAAAGACGAAGGTATTGTATTTCGAGACGGTGGCGAACCCGACACTGACGGTGGCGAATGTCCCCGCGCTCAGCCGGATGGCGCACAAGAAGGGGGTGACAGTGGTGGTCGATAATACGTTCTCGCCCATGATCATCTCACCGGTGAGGCTCGGTGCTGACATCGTGGTTCATAGCTGCTCCAAGTTTATCAGCGGCGGAGCAGACATTATTGCTGGTGCTGTTTGTGGGTCAAAAAGTTTGGTGAATTCTATGATGGACCTTCACCAAGGCACACTGATGCTTCTTGGGCCAACAATGAATGCTAAAGTTGCATCTGAACTCTCTCAAAGAATCCCCCATTTAAGTCTTCGAATGAAAGAGCACTCTCACAGAGCTTTAGTTTTTGCTCAAAGAATGAAGAATCTCGGCCTCAAAGTTATATACCCTGGTCTCAACGATCACCCCGACCACCATCTTCTAAGCTCCATAGCCAACAAAGACTACGGTTATGGCGGAGTTTTGTGCATCGACATGGGGACCGAAGAGAGAGCCAATAGCCTCATGAATATTCTTCAAAACCATACACATTTTGGTCTCATGGCAGTTAGCTTGGGCTTCTTCGAGACCCTCATGTCATGCTCAGGTAGTAGCACGAGTAGCGAGTTGAACGGCGAGGAGAAGGAATTGGCTGGCATTTCTCCAGGATTGATTAGGATGTCTGTTGGATATTTTGGAACCTTAGAGCAGAAATGGAGCCAATTGGAGAAGGCACTGTTGAAGATGAAGATTTGA

Coding sequence (CDS)

ATGATAACAGAAGTAAAAGCTCACAACACTTCTCTTCCATGTTGGAACAACAACAATGATAAGTTCAGTTACGACACGACATTGGCTCCATCATGGCCAATGAGAGAGGAGGATCCAGTGGCCGCTTTGGCAATGGCCCGCCCCGAGTTCGGAGAGCACGGTGGTGTGAACTTGTCGATCGAAAACTCAATAACATTCACGGTGATGGAGGCCGAAACGATGCGTAAGATGTTCGAAGGAGAGTTGGGGCCTGAACGGGACTTCTTCATCTATAGCCGCCACTTCCACCCGACCGTCATGGCCCTTAGCCGGCAGATGGCGGCGCTGGAAGGGACCGAGGCGGCATATTGCACGTCGAGTGGGATGGCTGCCATTTCATCCGTTTTGTTGCAGCTTGTGAACTCCGGCGGGCATATCGTGGCATCGAGAACGCTCTACGGCGGCACTCACGCGTTGCTGTCGCATTTTCTGCCGAGGGTCTGCAACATAACGACGACGTTTGTAGACGTGAAGGAGCATGAAATGGTTAGAGATGCCATGGTAGAGGGAAAGACGAAGGTATTGTATTTCGAGACGGTGGCGAACCCGACACTGACGGTGGCGAATGTCCCCGCGCTCAGCCGGATGGCGCACAAGAAGGGGGTGACAGTGGTGGTCGATAATACGTTCTCGCCCATGATCATCTCACCGGTGAGGCTCGGTGCTGACATCGTGGTTCATAGCTGCTCCAAGTTTATCAGCGGCGGAGCAGACATTATTGCTGGTGCTGTTTGTGGGTCAAAAAGTTTGGTGAATTCTATGATGGACCTTCACCAAGGCACACTGATGCTTCTTGGGCCAACAATGAATGCTAAAGTTGCATCTGAACTCTCTCAAAGAATCCCCCATTTAAGTCTTCGAATGAAAGAGCACTCTCACAGAGCTTTAGTTTTTGCTCAAAGAATGAAGAATCTCGGCCTCAAAGTTATATACCCTGGTCTCAACGATCACCCCGACCACCATCTTCTAAGCTCCATAGCCAACAAAGACTACGGTTATGGCGGAGTTTTGTGCATCGACATGGGGACCGAAGAGAGAGCCAATAGCCTCATGAATATTCTTCAAAACCATACACATTTTGGTCTCATGGCAGTTAGCTTGGGCTTCTTCGAGACCCTCATGTCATGCTCAGGTAGTAGCACGAGTAGCGAGTTGAACGGCGAGGAGAAGGAATTGGCTGGCATTTCTCCAGGATTGATTAGGATGTCTGTTGGATATTTTGGAACCTTAGAGCAGAAATGGAGCCAATTGGAGAAGGCACTGTTGAAGATGAAGATTTGA

Protein sequence

MITEVKAHNTSLPCWNNNNDKFSYDTTLAPSWPMREEDPVAALAMARPEFGEHGGVNLSIENSITFTVMEAETMRKMFEGELGPERDFFIYSRHFHPTVMALSRQMAALEGTEAAYCTSSGMAAISSVLLQLVNSGGHIVASRTLYGGTHALLSHFLPRVCNITTTFVDVKEHEMVRDAMVEGKTKVLYFETVANPTLTVANVPALSRMAHKKGVTVVVDNTFSPMIISPVRLGADIVVHSCSKFISGGADIIAGAVCGSKSLVNSMMDLHQGTLMLLGPTMNAKVASELSQRIPHLSLRMKEHSHRALVFAQRMKNLGLKVIYPGLNDHPDHHLLSSIANKDYGYGGVLCIDMGTEERANSLMNILQNHTHFGLMAVSLGFFETLMSCSGSSTSSELNGEEKELAGISPGLIRMSVGYFGTLEQKWSQLEKALLKMKI
Homology
BLAST of Cla97C01G001520 vs. NCBI nr
Match: XP_038875936.1 (methionine gamma-lyase-like [Benincasa hispida])

HSP 1 Score: 802.7 bits (2072), Expect = 1.5e-228
Identity = 403/443 (90.97%), Postives = 419/443 (94.58%), Query Frame = 0

Query: 1   MITEVKAHNTSLPCWNNNNDKFSYD----TTLAPSWPMREEDPVAALAMARPEFGEHGGV 60
           M TEVKAH+TSLPCWN +N KFSY     T +  SWPM +EDP A LAMARPEFGEHGGV
Sbjct: 1   MTTEVKAHDTSLPCWNIDNKKFSYQTKSATPMVTSWPMGDEDPAATLAMARPEFGEHGGV 60

Query: 61  NLSIENSITFTVMEAETMRKMFEGELGPERDFFIYSRHFHPTVMALSRQMAALEGTEAAY 120
           N+SIENSITFTVMEAETMR MFEGELGPERDFFIYSRHF+PTV+ALSRQMAALEGTEAAY
Sbjct: 61  NMSIENSITFTVMEAETMRMMFEGELGPERDFFIYSRHFNPTVLALSRQMAALEGTEAAY 120

Query: 121 CTSSGMAAISSVLLQLVNSGGHIVASRTLYGGTHALLSHFLPRVCNITTTFVDVKEHEMV 180
           CTSSGMAA+SSVLLQLV+SGGHIVASRTLYGGTHALLSHFLPRVCNITTTFVDVK+HEMV
Sbjct: 121 CTSSGMAAVSSVLLQLVSSGGHIVASRTLYGGTHALLSHFLPRVCNITTTFVDVKDHEMV 180

Query: 181 RDAMVEGKTKVLYFETVANPTLTVANVPALSRMAHKKGVTVVVDNTFSPMIISPVRLGAD 240
           RDAMVEGKTKVLYFETVANPTLTVANVP LSRMAH+KGVTVVVDNTFSPMIISP RLGAD
Sbjct: 181 RDAMVEGKTKVLYFETVANPTLTVANVPELSRMAHEKGVTVVVDNTFSPMIISPARLGAD 240

Query: 241 IVVHSCSKFISGGADIIAGAVCGSKSLVNSMMDLHQGTLMLLGPTMNAKVASELSQRIPH 300
           +VVHSCSKFISGGADIIAGAVCGSK+LVNSMMDLHQGTLMLLGPTMNAKVASE+SQRIPH
Sbjct: 241 VVVHSCSKFISGGADIIAGAVCGSKNLVNSMMDLHQGTLMLLGPTMNAKVASEISQRIPH 300

Query: 301 LSLRMKEHSHRALVFAQRMKNLGLKVIYPGLNDHPDHHLLSSIANKDYGYGGVLCIDMGT 360
           L LRMKEHSHRALVFAQRMKNLGLKV YPGLNDHPDH LLS IANKDYGYGGVLCIDMGT
Sbjct: 301 LGLRMKEHSHRALVFAQRMKNLGLKVTYPGLNDHPDHQLLSYIANKDYGYGGVLCIDMGT 360

Query: 361 EERANSLMNILQNHTHFGLMAVSLGFFETLMSCSGSSTSSELNGEEKELAGISPGLIRMS 420
           EERAN LMN+LQNHTHFGLMAVSLGFFETLMSCSGSSTSSELN +EKELAGISPGLIRMS
Sbjct: 361 EERANRLMNLLQNHTHFGLMAVSLGFFETLMSCSGSSTSSELNSKEKELAGISPGLIRMS 420

Query: 421 VGYFGTLEQKWSQLEKALLKMKI 440
           VGY GTLEQKWSQLEKALLKMKI
Sbjct: 421 VGYVGTLEQKWSQLEKALLKMKI 443

BLAST of Cla97C01G001520 vs. NCBI nr
Match: XP_004143943.3 (methionine gamma-lyase [Cucumis sativus] >KGN49981.1 hypothetical protein Csa_000014 [Cucumis sativus])

HSP 1 Score: 793.5 bits (2048), Expect = 9.3e-226
Identity = 404/444 (90.99%), Postives = 419/444 (94.37%), Query Frame = 0

Query: 1   MITEVKAHNTSLPCWNNNNDKFSYDT-----TLAPSWPMREEDPVAALAMARPEFGEHGG 60
           MITEV+AH+TSLPC NNNN  FSYDT     T   SWPM E DP AALAMA PEFGEHGG
Sbjct: 1   MITEVRAHDTSLPCKNNNN--FSYDTEPTSITRLSSWPMGELDPAAALAMASPEFGEHGG 60

Query: 61  VNLSIENSITFTVMEAETMRKMFEGELGPERDFFIYSRHFHPTVMALSRQMAALEGTEAA 120
           VN+SIENSITFTVMEAETMR+MFEGELGPERDFFIYSRHF+PTVMALSRQMAALEGTEAA
Sbjct: 61  VNMSIENSITFTVMEAETMRRMFEGELGPERDFFIYSRHFNPTVMALSRQMAALEGTEAA 120

Query: 121 YCTSSGMAAISSVLLQLVNSGGHIVASRTLYGGTHALLSHFLPRVCNITTTFVDVKEHEM 180
           YCTSSGMAA+SSVLLQLV+SGGHIVASRTLYGGTHALLSHFLPRV  ITTTFVDV+ HEM
Sbjct: 121 YCTSSGMAAVSSVLLQLVSSGGHIVASRTLYGGTHALLSHFLPRVSKITTTFVDVRNHEM 180

Query: 181 VRDAMVEGKTKVLYFETVANPTLTVANVPALSRMAHKKGVTVVVDNTFSPMIISPVRLGA 240
           VRDAMVEGKTKVLYFETVANPTLTVAN+P LSRMAH+KGVTVVVDNTFSP+IISP RLGA
Sbjct: 181 VRDAMVEGKTKVLYFETVANPTLTVANIPELSRMAHQKGVTVVVDNTFSPIIISPTRLGA 240

Query: 241 DIVVHSCSKFISGGADIIAGAVCGSKSLVNSMMDLHQGTLMLLGPTMNAKVASELSQRIP 300
           D+VVHSCSKFISGGADIIAGAVCGSK LVNSMMDLHQGTLMLLGPTMNAKVASELSQRIP
Sbjct: 241 DVVVHSCSKFISGGADIIAGAVCGSKELVNSMMDLHQGTLMLLGPTMNAKVASELSQRIP 300

Query: 301 HLSLRMKEHSHRALVFAQRMKNLGLKVIYPGLNDHPDHHLLSSIANKDYGYGGVLCIDMG 360
           HLSLRMKEHS+RALVFAQRMKNLGLKVIYPGLNDHPDH LL SIANKDYGYGGVLCIDMG
Sbjct: 301 HLSLRMKEHSNRALVFAQRMKNLGLKVIYPGLNDHPDHQLLGSIANKDYGYGGVLCIDMG 360

Query: 361 TEERANSLMNILQNHTHFGLMAVSLGFFETLMSCSGSSTSSELNGEEKELAGISPGLIRM 420
           TEERAN LMN+LQNHTHFGLMAVSLGFFETLMSCSGSSTSSELNGEEKELAGISPGLIRM
Sbjct: 361 TEERANRLMNLLQNHTHFGLMAVSLGFFETLMSCSGSSTSSELNGEEKELAGISPGLIRM 420

Query: 421 SVGYFGTLEQKWSQLEKALLKMKI 440
           SVGY GTLEQKW+QLEKALLKMKI
Sbjct: 421 SVGYVGTLEQKWNQLEKALLKMKI 442

BLAST of Cla97C01G001520 vs. NCBI nr
Match: XP_008437446.1 (PREDICTED: methionine gamma-lyase-like [Cucumis melo] >KAA0042648.1 methionine gamma-lyase-like [Cucumis melo var. makuwa] >TYK06049.1 methionine gamma-lyase-like [Cucumis melo var. makuwa])

HSP 1 Score: 786.9 bits (2031), Expect = 8.7e-224
Identity = 403/444 (90.77%), Postives = 417/444 (93.92%), Query Frame = 0

Query: 1   MIT-EVKAHNTSLPCWNNNNDKFSYDT-----TLAPSWPMREEDPVAALAMARPEFGEHG 60
           MIT +VKAH+TSLPC NNN   FSYDT     T   SWPM + DP AALAMARPEFGEHG
Sbjct: 1   MITDQVKAHDTSLPCKNNN---FSYDTKPTSITRLTSWPMGKVDPAAALAMARPEFGEHG 60

Query: 61  GVNLSIENSITFTVMEAETMRKMFEGELGPERDFFIYSRHFHPTVMALSRQMAALEGTEA 120
           GVN+SIENSITFTVMEAETMR+MFEGELGPERDFFIYSRHF+PTVMALSRQMAALEGTEA
Sbjct: 61  GVNMSIENSITFTVMEAETMRRMFEGELGPERDFFIYSRHFNPTVMALSRQMAALEGTEA 120

Query: 121 AYCTSSGMAAISSVLLQLVNSGGHIVASRTLYGGTHALLSHFLPRVCNITTTFVDVKEHE 180
           AYCTSSGMAA+SSVLLQLV+SGGHIVASRTLYGGTHALLSHFLPRV  ITTTFVDV+ HE
Sbjct: 121 AYCTSSGMAAVSSVLLQLVSSGGHIVASRTLYGGTHALLSHFLPRVSKITTTFVDVRNHE 180

Query: 181 MVRDAMVEGKTKVLYFETVANPTLTVANVPALSRMAHKKGVTVVVDNTFSPMIISPVRLG 240
           MVRDAMVEGKTKVLYFETVANPTLTVAN+P LSRMAH+KGVTVVVDNTFSPMIISP RLG
Sbjct: 181 MVRDAMVEGKTKVLYFETVANPTLTVANIPELSRMAHEKGVTVVVDNTFSPMIISPARLG 240

Query: 241 ADIVVHSCSKFISGGADIIAGAVCGSKSLVNSMMDLHQGTLMLLGPTMNAKVASELSQRI 300
           AD+VVHSCSKFISGGADIIAGAVCGSK LVNSMMDLHQGTLMLLGPTMNAKVASELSQRI
Sbjct: 241 ADVVVHSCSKFISGGADIIAGAVCGSKDLVNSMMDLHQGTLMLLGPTMNAKVASELSQRI 300

Query: 301 PHLSLRMKEHSHRALVFAQRMKNLGLKVIYPGLNDHPDHHLLSSIANKDYGYGGVLCIDM 360
           PHL LRMKEHS+RALVFAQRMKNLGLKVIYPGLNDHPDH LL SIANKDYGYGGVLCIDM
Sbjct: 301 PHLGLRMKEHSNRALVFAQRMKNLGLKVIYPGLNDHPDHQLLGSIANKDYGYGGVLCIDM 360

Query: 361 GTEERANSLMNILQNHTHFGLMAVSLGFFETLMSCSGSSTSSELNGEEKELAGISPGLIR 420
           GTEERAN LMN+LQNHTHFGLMAVSLGFFETLMSCSGSSTSSELNGEEKELAGISPGLIR
Sbjct: 361 GTEERANRLMNLLQNHTHFGLMAVSLGFFETLMSCSGSSTSSELNGEEKELAGISPGLIR 420

Query: 421 MSVGYFGTLEQKWSQLEKALLKMK 439
           MSVGY GTLEQKWSQLEKALLKMK
Sbjct: 421 MSVGYVGTLEQKWSQLEKALLKMK 441

BLAST of Cla97C01G001520 vs. NCBI nr
Match: KAG7017043.1 (Methionine gamma-lyase, partial [Cucurbita argyrosperma subsp. argyrosperma])

HSP 1 Score: 748.0 bits (1930), Expect = 4.5e-212
Identity = 378/429 (88.11%), Postives = 399/429 (93.01%), Query Frame = 0

Query: 15  WNNNNDKFSYDTTLAP---SW-PMREEDPVAALAMARPEFGEHGGVNLSIENSITFTVME 74
           W+  N +  +     P   +W P  EEDP A+LA ARPEFGEHGGVNLSIENSITFTVME
Sbjct: 8   WDVKNKRLKHTAPPPPPPVTWRPEGEEDPAASLAAARPEFGEHGGVNLSIENSITFTVME 67

Query: 75  AETMRKMFEGELGPERDFFIYSRHFHPTVMALSRQMAALEGTEAAYCTSSGMAAISSVLL 134
           AETMRKMFEGELGPE+DFFIYSRHF+PTVMAL RQMAA+EGTEAAYCTSSGMAAISSVLL
Sbjct: 68  AETMRKMFEGELGPEQDFFIYSRHFNPTVMALGRQMAAIEGTEAAYCTSSGMAAISSVLL 127

Query: 135 QLVNSGGHIVASRTLYGGTHALLSHFLPRVCNITTTFVDVKEHEMVRDAMVEGKTKVLYF 194
           QLV+SGGHIVASRTLYGGTHALLSHFLPRVCNITT+FVDVK+H  VRDAMV+ KTKVLYF
Sbjct: 128 QLVSSGGHIVASRTLYGGTHALLSHFLPRVCNITTSFVDVKDHAKVRDAMVKDKTKVLYF 187

Query: 195 ETVANPTLTVANVPALSRMAHKKGVTVVVDNTFSPMIISPVRLGADIVVHSCSKFISGGA 254
           ETVANPTLTVAN+P LSRMAH+KGVTVVVDNTFSPMIISP RLGAD+VVHSCSKFISGGA
Sbjct: 188 ETVANPTLTVANIPELSRMAHEKGVTVVVDNTFSPMIISPTRLGADVVVHSCSKFISGGA 247

Query: 255 DIIAGAVCGSKSLVNSMMDLHQGTLMLLGPTMNAKVASELSQRIPHLSLRMKEHSHRALV 314
           DIIAGAVCGSK LVNSMMDLHQGTLMLLGPTMNAKVASELSQR+PHL+LRMKEHSHRALV
Sbjct: 248 DIIAGAVCGSKKLVNSMMDLHQGTLMLLGPTMNAKVASELSQRVPHLALRMKEHSHRALV 307

Query: 315 FAQRMKNLGLKVIYPGLNDHPDHHLLSSIANKDYGYGGVLCIDMGTEERANSLMNILQNH 374
           FAQRMKNLGLKVIYPGL DHPDHHLLSSIANKDYGYGGVLCIDMGTEERAN LMN+LQNH
Sbjct: 308 FAQRMKNLGLKVIYPGLKDHPDHHLLSSIANKDYGYGGVLCIDMGTEERANRLMNLLQNH 367

Query: 375 THFGLMAVSLGFFETLMSCSGSSTSSELNGEEKELAGISPGLIRMSVGYFGTLEQKWSQL 434
           THFGL+AVSLG+FETLMSCSGSSTSSE+N EEKELAGISPGLIRMSVGY GTLEQKWSQL
Sbjct: 368 THFGLLAVSLGYFETLMSCSGSSTSSEMNVEEKELAGISPGLIRMSVGYVGTLEQKWSQL 427

Query: 435 EKALLKMKI 440
           EKALLKM I
Sbjct: 428 EKALLKMNI 436

BLAST of Cla97C01G001520 vs. NCBI nr
Match: KAG6579584.1 (Methionine gamma-lyase, partial [Cucurbita argyrosperma subsp. sororia])

HSP 1 Score: 743.4 bits (1918), Expect = 1.1e-210
Identity = 377/429 (87.88%), Postives = 397/429 (92.54%), Query Frame = 0

Query: 15  WNNNNDKFSYDTTLAP---SW-PMREEDPVAALAMARPEFGEHGGVNLSIENSITFTVME 74
           W+  N +  +     P   +W P  EEDP A+LA ARPEFGEHGGVNLSIENSITFTVME
Sbjct: 8   WDVKNKRLKHTAPPPPPPVTWRPEGEEDPAASLAAARPEFGEHGGVNLSIENSITFTVME 67

Query: 75  AETMRKMFEGELGPERDFFIYSRHFHPTVMALSRQMAALEGTEAAYCTSSGMAAISSVLL 134
           AETM KMFEGELGPE+DFFIYSRHF+PTVMAL RQMAALEGTEAAYCTSSGMAAISSVLL
Sbjct: 68  AETMSKMFEGELGPEQDFFIYSRHFNPTVMALGRQMAALEGTEAAYCTSSGMAAISSVLL 127

Query: 135 QLVNSGGHIVASRTLYGGTHALLSHFLPRVCNITTTFVDVKEHEMVRDAMVEGKTKVLYF 194
           QLV+SGGHIVASRTLYGGTHALLSHFLPRVCNITT+FVDVK+H  VRDAMV+ KTKVLYF
Sbjct: 128 QLVSSGGHIVASRTLYGGTHALLSHFLPRVCNITTSFVDVKDHAKVRDAMVKDKTKVLYF 187

Query: 195 ETVANPTLTVANVPALSRMAHKKGVTVVVDNTFSPMIISPVRLGADIVVHSCSKFISGGA 254
           ETVANPTLTVAN+P LSRMAH+KGVTVVVDNTFSPMIISP RLGAD+VVHSCSKFISG A
Sbjct: 188 ETVANPTLTVANIPELSRMAHEKGVTVVVDNTFSPMIISPTRLGADVVVHSCSKFISGRA 247

Query: 255 DIIAGAVCGSKSLVNSMMDLHQGTLMLLGPTMNAKVASELSQRIPHLSLRMKEHSHRALV 314
           DIIAGAVCGSK LVNSMMDLHQGTLMLLGPTMNAKVASELSQR+PHL+LRMKEHSHRALV
Sbjct: 248 DIIAGAVCGSKKLVNSMMDLHQGTLMLLGPTMNAKVASELSQRVPHLALRMKEHSHRALV 307

Query: 315 FAQRMKNLGLKVIYPGLNDHPDHHLLSSIANKDYGYGGVLCIDMGTEERANSLMNILQNH 374
           FAQRMKNLGLKVIYPGL DHPDHHLLSSIANKDYGYGGVLCIDMGTEERAN LMN+LQNH
Sbjct: 308 FAQRMKNLGLKVIYPGLKDHPDHHLLSSIANKDYGYGGVLCIDMGTEERANRLMNLLQNH 367

Query: 375 THFGLMAVSLGFFETLMSCSGSSTSSELNGEEKELAGISPGLIRMSVGYFGTLEQKWSQL 434
           THFGL+AVSLG+FETLMSCSGSSTSSE+N EEKELAGISPGLIRMSVGY GTLEQKWSQL
Sbjct: 368 THFGLLAVSLGYFETLMSCSGSSTSSEMNVEEKELAGISPGLIRMSVGYVGTLEQKWSQL 427

Query: 435 EKALLKMKI 440
           EKALLKM I
Sbjct: 428 EKALLKMNI 436

BLAST of Cla97C01G001520 vs. ExPASy Swiss-Prot
Match: Q9SGU9 (Methionine gamma-lyase OS=Arabidopsis thaliana OX=3702 GN=MGL PE=1 SV=1)

HSP 1 Score: 614.8 bits (1584), Expect = 7.7e-175
Identity = 299/402 (74.38%), Postives = 352/402 (87.56%), Query Frame = 0

Query: 36  EEDPVAALAMARPEFGEHGGVNLSIENSITFTVMEAETMRKMFEGELGPERDFFIYSRHF 95
           + DP AA+A  R EFGEHGGVN+SIE S TFTVME +TMR+MF GELGP+ DF++YSRHF
Sbjct: 40  DADPAAAIANIRHEFGEHGGVNMSIEASATFTVMEPDTMRRMFTGELGPDNDFYVYSRHF 99

Query: 96  HPTVMALSRQMAALEGTEAAYCTSSGMAAISSVLLQLVNSGGHIVASRTLYGGTHALLSH 155
           +PTV+ LSRQMAALEGT+AAYCTSSGM+AISSV+LQL +SGGH+VA+ TLYGGTHALLSH
Sbjct: 100 NPTVLNLSRQMAALEGTQAAYCTSSGMSAISSVMLQLCSSGGHVVAASTLYGGTHALLSH 159

Query: 156 FLPRVCNITTTFVDVKEHEMVRDAMVEGKTKVLYFETVANPTLTVANVPALSRMAHKKGV 215
           FLPR CNITT+FVD+ +H  V +A+VEG+T+VLYFE+VANPTLTVA++P LSRMAH+KGV
Sbjct: 160 FLPRTCNITTSFVDITDHGAVANAIVEGRTQVLYFESVANPTLTVADIPELSRMAHEKGV 219

Query: 216 TVVVDNTFSPMIISPVRLGADIVVHSCSKFISGGADIIAGAVCGSKSLVNSMMDLHQGTL 275
           TVVVDNTF+PM++SP +LGAD+VVHS SKFISGGADIIAGAVCGS++LV  MMDL  G+L
Sbjct: 220 TVVVDNTFAPMVLSPAKLGADVVVHSISKFISGGADIIAGAVCGSENLVKEMMDLRGGSL 279

Query: 276 MLLGPTMNAKVASELSQRIPHLSLRMKEHSHRALVFAQRMKNLGLKVIYPGLNDHPDHHL 335
           MLLGPTMNAKVA ELS+RIPHL LRM+EHSHRA V+A+RM++LG+KVIYPGL  HP H L
Sbjct: 280 MLLGPTMNAKVAFELSERIPHLGLRMREHSHRAQVYAERMRDLGMKVIYPGLETHPQHKL 339

Query: 336 LSSIANKDYGYGGVLCIDMGTEERANSLMNILQNHTHFGLMAVSLGFFETLMSCSGSSTS 395
              + N+DYGYGG+L IDM TEE+AN LM  LQN T FG MAVSLG++ETLMSCSGSSTS
Sbjct: 340 FKGMVNRDYGYGGLLSIDMETEEKANKLMAYLQNATQFGFMAVSLGYYETLMSCSGSSTS 399

Query: 396 SELNGEEKELAGISPGLIRMSVGYFGTLEQKWSQLEKALLKM 438
           SEL+  +KE AGISPGL+RMSVGY GTLEQKW+Q EKA L+M
Sbjct: 400 SELDPSQKEAAGISPGLVRMSVGYVGTLEQKWTQFEKAFLRM 441

BLAST of Cla97C01G001520 vs. ExPASy Swiss-Prot
Match: Q8L0X4 (L-methionine gamma-lyase OS=Fusobacterium nucleatum subsp. polymorphum OX=76857 GN=mgl PE=1 SV=1)

HSP 1 Score: 197.6 bits (501), Expect = 3.0e-49
Identity = 146/392 (37.24%), Postives = 218/392 (55.61%), Query Frame = 0

Query: 53  HGGVNLSIENSITFTVMEAETMRKMFEGELGPERDFFIYSRHFHPTVMALSRQMAALEGT 112
           +G + + I  + TF    AE   + F      E   +IY+R  +PT   L  ++AALE  
Sbjct: 22  YGTLAMPIYQTSTFIFDSAEQGGRRF----ALEEAGYIYTRLGNPTTTVLENKIAALEEG 81

Query: 113 EAAYCTSSGMAAISSVLLQLVNSGGHIVASRTLYGGTHALLSHFLPRVCNITTTFVDVKE 172
           EAA  TSSGM AISS L  ++ +G H+V  +TLYG T AL+ H L R   I  TFVD   
Sbjct: 82  EAAVATSSGMGAISSTLWTVLKAGDHVVTDKTLYGCTFALMCHGLTRF-GIEVTFVDTSN 141

Query: 173 HEMVRDAMVEGKTKVLYFETVANPTLTVANVPALSRMAHKKGVT-VVVDNTF-SPMIISP 232
            + V++AM +  T+V+Y ET ANP L + ++ ALS++AH    T V+VDNTF +P +  P
Sbjct: 142 LDEVKNAM-KKNTRVVYLETPANPNLKIVDLEALSKLAHTNPNTLVIVDNTFATPYMQKP 201

Query: 233 VRLGADIVVHSCSKFISGGADIIAGAVCGSKSLVNSMMDLHQGTLMLLGPTMNAKVASEL 292
           ++LGADIVVHS +K+I+G  D+IAG V  +K L + +  +  G   + G  +  + A  +
Sbjct: 202 LKLGADIVVHSVTKYINGHGDVIAGLVITNKELADQIRFI--GLKDMTGAVLGPQDAYYI 261

Query: 293 SQRIPHLSLRMKEH---SHRALVFAQRMKNLGLKVIYPGLNDHPDHHLLSSIANKDY-GY 352
            + +    +RM+ H   + + + F  +   +  +V YPGL  HP H     IA K    +
Sbjct: 262 IRGMKTFEIRMERHCKNAKKVVEFLNKHPKIE-RVYYPGLETHPGH----EIAKKQMKDF 321

Query: 353 GGVLCIDM-GTEERANSLMNILQNHTHFGLMAVSLGFFETLMSCSGSSTSSELNGEEKEL 412
           G ++  ++ G  E   +L+N L+       +AVSLG  ETL+    S T S    EE+E 
Sbjct: 322 GAMISFELKGGFEAGKTLLNNLK----LCSLAVSLGDTETLIQHPASMTHSPYTKEEREA 381

Query: 413 AGISPGLIRMSVGYFGTLEQKWSQLEKALLKM 438
           AGI+ GL+R+SVG    +E   + LE+ L K+
Sbjct: 382 AGITDGLVRLSVG-LENVEDIIADLEQGLEKI 395

BLAST of Cla97C01G001520 vs. ExPASy Swiss-Prot
Match: Q8RDT4 (L-methionine gamma-lyase OS=Fusobacterium nucleatum subsp. nucleatum (strain ATCC 25586 / DSM 15643 / BCRC 10681 / CIP 101130 / JCM 8532 / KCTC 2640 / LMG 13131 / VPI 4355) OX=190304 GN=FN1419 PE=1 SV=1)

HSP 1 Score: 190.7 bits (483), Expect = 3.6e-47
Identity = 141/391 (36.06%), Postives = 212/391 (54.22%), Query Frame = 0

Query: 53  HGGVNLSIENSITFTVMEAETMRKMFEGELGPERDFFIYSRHFHPTVMALSRQMAALEGT 112
           +G + + I  + TF    AE   + F      E   +IY+R  +PT   L  ++AALE  
Sbjct: 22  YGTLAMPIYQTSTFIFDSAEQGGRRF----ALEEAGYIYTRLGNPTTTVLENKIAALEEG 81

Query: 113 EAAYCTSSGMAAISSVLLQLVNSGGHIVASRTLYGGTHALLSHFLPRVCNITTTFVDVKE 172
           EA    SSGM AISS L  ++ +G H+V  +TLYG T AL++H L R   +  TFVD   
Sbjct: 82  EAGIAMSSGMGAISSTLWTVLKAGDHVVTDKTLYGCTFALMNHGLTRF-GVEVTFVDTSN 141

Query: 173 HEMVRDAMVEGKTKVLYFETVANPTLTVANVPALSRMAHKKGVT-VVVDNTF-SPMIISP 232
            E V++AM +  T+V+Y ET ANP L + ++ ALS++AH    T V+VDNTF +P +  P
Sbjct: 142 LEEVKNAM-KKNTRVVYLETPANPNLKIVDLEALSKIAHTNPNTLVIVDNTFATPYMQKP 201

Query: 233 VRLGADIVVHSCSKFISGGADIIAGAVCGSKSLVNSMMDLHQGTLMLLGPTMNAKVASEL 292
           ++LG DIVVHS +K+++G  D+IAG V   + L + +  +  G   + G  +  + A  +
Sbjct: 202 LKLGVDIVVHSATKYLNGHGDVIAGLVVTRQELADQIRFV--GLKDMTGAVLGPQEAYYI 261

Query: 293 SQRIPHLSLRMKEHSHRALVFAQRMKNLGL--KVIYPGLNDHPDHHLLSSIANKDY-GYG 352
            + +    +RM+ H   A      +       KV YPGL  HP +     IA K    +G
Sbjct: 262 IRGLKTFEIRMERHCKNARTIVDFLNKHPKVEKVYYPGLETHPGY----EIAKKQMKDFG 321

Query: 353 GVLCIDM-GTEERANSLMNILQNHTHFGLMAVSLGFFETLMSCSGSSTSSELNGEEKELA 412
            ++  ++ G  E   +L+N L+       +AVSLG  ETL+    S T S    EE+E+A
Sbjct: 322 AMISFELKGGFEAGKTLLNNLK----LCSLAVSLGDTETLIQHPASMTHSPYTKEEREVA 381

Query: 413 GISPGLIRMSVGYFGTLEQKWSQLEKALLKM 438
           GI+ GL+R+SVG    +E   + LE+ L K+
Sbjct: 382 GITDGLVRLSVG-LENVEDIIADLEQGLEKI 395

BLAST of Cla97C01G001520 vs. ExPASy Swiss-Prot
Match: Q7MX71 (L-methionine gamma-lyase OS=Porphyromonas gingivalis (strain ATCC BAA-308 / W83) OX=242619 GN=mgl PE=1 SV=2)

HSP 1 Score: 186.8 bits (473), Expect = 5.2e-46
Identity = 138/365 (37.81%), Postives = 201/365 (55.07%), Query Frame = 0

Query: 60  IENSITFTVMEAETMRKMFEGELGPERDFFIYSRHFHPTVMALSRQMAALEGTEAAYCTS 119
           I  + TF    AE   + F G    E D +IY+R  +P    +  ++A LEG EAA   S
Sbjct: 32  IYQTSTFVFDTAEQGGRRFAG----EEDGYIYTRLGNPNCTQVEEKLAMLEGGEAAASAS 91

Query: 120 SGMAAISSVLLQLVNSGGHIVASRTLYGGTHALLSHFLPRVCNITTTFVDVKEHEMVRDA 179
           SG+ AISS +   V +G HIVA +TLYG T A L+H L R   +  T VD +  E V +A
Sbjct: 92  SGIGAISSAIWVCVKAGDHIVAGKTLYGCTFAFLTHGLSRY-GVEVTLVDTRHPEEV-EA 151

Query: 180 MVEGKTKVLYFETVANPTLTVANVPALSRMAHK-KGVTVVVDNTF-SPMIISPVRLGADI 239
            +   TK++Y ET ANP + + ++ A+  +AHK +GV V+VDNT+ +P I  P+ LGADI
Sbjct: 152 AIRPNTKLVYLETPANPNMYLTDIKAVCDIAHKHEGVRVMVDNTYCTPYICRPLELGADI 211

Query: 240 VVHSCSKFISGGADIIAGAVCGSKSLVNSMMDLHQGTLMLLGPTMNAKVASELSQRIPHL 299
           VVHS +K+++G  D+IAG V G +  +  +  +  G   L G  M+   A  +S+ +  L
Sbjct: 212 VVHSATKYLNGHGDVIAGFVVGKEDYIKEVKLV--GVKDLTGANMSPFDAYLISRGMKTL 271

Query: 300 SLRMKEHSHRALVFAQRM-KNLGLKVIY-PGLNDHPDHHLLSSIANKDYGY-GGVLCIDM 359
            +RM++H   A   A+ + K+  ++ +Y PGL   P + L    A K     G ++  ++
Sbjct: 272 QIRMEQHCRNAQTVAEFLEKHPAVEAVYFPGLPSFPQYEL----AKKQMALPGAMIAFEV 331

Query: 360 -GTEERANSLMNILQNHTHFGLMAVSLGFFETLMSCSGSSTSSELNGEEKELAGISPGLI 419
            G  E    LMN L    H   +AVSLG  ETL+    S T S    EE+  + IS GL+
Sbjct: 332 KGGCEAGKKLMNNL----HLCSLAVSLGDTETLIQHPASMTHSPYTPEERAASDISEGLV 380

BLAST of Cla97C01G001520 vs. ExPASy Swiss-Prot
Match: O05394 (Cystathionine gamma-lyase OS=Bacillus subtilis (strain 168) OX=224308 GN=mccB PE=1 SV=1)

HSP 1 Score: 181.8 bits (460), Expect = 1.7e-44
Identity = 128/371 (34.50%), Postives = 204/371 (54.99%), Query Frame = 0

Query: 53  HGGVNLSIE-NSITFTVMEAETMRKMFEGELGPERDFFIYSRHFHPTVMALSRQMAALEG 112
           HGG+    +  +++  + +  T ++   G    +   + YSR  +PT  AL   +  LE 
Sbjct: 9   HGGITGDEKTGAVSVPIYQVSTYKQPKAG----QHTGYEYSRTANPTRTALEALVTELES 68

Query: 113 TEAAYCTSSGMAAISSVLLQLVNSGGHIVASRTLYGGTHALLSHFLPRVCNITTTFVDVK 172
            EA Y  SSGMAAI++V++ L NSG H+V +  +YGGT+ +++  L R+  I +TFVD  
Sbjct: 69  GEAGYAFSSGMAAITAVMM-LFNSGDHVVLTDDVYGGTYRVMTKVLNRL-GIESTFVDTS 128

Query: 173 EHEMVRDAMVEGKTKVLYFETVANPTLTVANVPALSRMAHKKGVTVVVDNTF-SPMIISP 232
             E V  A +   TK +Y ET  NP L + ++  ++ +A K GV ++VDNTF +P    P
Sbjct: 129 SREEVEKA-IRPNTKAIYIETPTNPLLKITDLTLMADIAKKAGVLLIVDNTFNTPYFQQP 188

Query: 233 VRLGADIVVHSCSKFISGGADIIAG-AVCGSKSLVNSMMDLHQGTLMLLGPTMNAKVASE 292
           + LGADIV+HS +K++ G +D++ G  V  SK L   +  +   T  +LGP    + +  
Sbjct: 189 LTLGADIVLHSATKYLGGHSDVVGGLVVTASKELGEELHFVQNSTGGVLGP----QDSWL 248

Query: 293 LSQRIPHLSLRMKEHSHRALVFAQRMKN--LGLKVIYPGLNDHPDHHLLSSIANKDYGYG 352
           L + I  L LRM+     A   A  ++N      + YPG ++HP H L  +   +  G+G
Sbjct: 249 LMRGIKTLGLRMEAIDQNARKIASFLENHPAVQTLYYPGSSNHPGHELAKT---QGAGFG 308

Query: 353 GVLCIDMGTEERANSLMNILQNHTHFGLMAVSLGFFETLMSCSGSSTSSELNGEEKELAG 412
           G++  D+G+EER ++ +  L+  T    +A SLG  E+L+S     T + +  E +   G
Sbjct: 309 GMISFDIGSEERVDAFLGNLKLFT----IAESLGAVESLISVPARMTHASIPRERRLELG 361

Query: 413 ISPGLIRMSVG 419
           I+ GLIR+SVG
Sbjct: 369 ITDGLIRISVG 361

BLAST of Cla97C01G001520 vs. ExPASy TrEMBL
Match: A0A0A0KQL7 (Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_5G148520 PE=3 SV=1)

HSP 1 Score: 793.5 bits (2048), Expect = 4.5e-226
Identity = 404/444 (90.99%), Postives = 419/444 (94.37%), Query Frame = 0

Query: 1   MITEVKAHNTSLPCWNNNNDKFSYDT-----TLAPSWPMREEDPVAALAMARPEFGEHGG 60
           MITEV+AH+TSLPC NNNN  FSYDT     T   SWPM E DP AALAMA PEFGEHGG
Sbjct: 1   MITEVRAHDTSLPCKNNNN--FSYDTEPTSITRLSSWPMGELDPAAALAMASPEFGEHGG 60

Query: 61  VNLSIENSITFTVMEAETMRKMFEGELGPERDFFIYSRHFHPTVMALSRQMAALEGTEAA 120
           VN+SIENSITFTVMEAETMR+MFEGELGPERDFFIYSRHF+PTVMALSRQMAALEGTEAA
Sbjct: 61  VNMSIENSITFTVMEAETMRRMFEGELGPERDFFIYSRHFNPTVMALSRQMAALEGTEAA 120

Query: 121 YCTSSGMAAISSVLLQLVNSGGHIVASRTLYGGTHALLSHFLPRVCNITTTFVDVKEHEM 180
           YCTSSGMAA+SSVLLQLV+SGGHIVASRTLYGGTHALLSHFLPRV  ITTTFVDV+ HEM
Sbjct: 121 YCTSSGMAAVSSVLLQLVSSGGHIVASRTLYGGTHALLSHFLPRVSKITTTFVDVRNHEM 180

Query: 181 VRDAMVEGKTKVLYFETVANPTLTVANVPALSRMAHKKGVTVVVDNTFSPMIISPVRLGA 240
           VRDAMVEGKTKVLYFETVANPTLTVAN+P LSRMAH+KGVTVVVDNTFSP+IISP RLGA
Sbjct: 181 VRDAMVEGKTKVLYFETVANPTLTVANIPELSRMAHQKGVTVVVDNTFSPIIISPTRLGA 240

Query: 241 DIVVHSCSKFISGGADIIAGAVCGSKSLVNSMMDLHQGTLMLLGPTMNAKVASELSQRIP 300
           D+VVHSCSKFISGGADIIAGAVCGSK LVNSMMDLHQGTLMLLGPTMNAKVASELSQRIP
Sbjct: 241 DVVVHSCSKFISGGADIIAGAVCGSKELVNSMMDLHQGTLMLLGPTMNAKVASELSQRIP 300

Query: 301 HLSLRMKEHSHRALVFAQRMKNLGLKVIYPGLNDHPDHHLLSSIANKDYGYGGVLCIDMG 360
           HLSLRMKEHS+RALVFAQRMKNLGLKVIYPGLNDHPDH LL SIANKDYGYGGVLCIDMG
Sbjct: 301 HLSLRMKEHSNRALVFAQRMKNLGLKVIYPGLNDHPDHQLLGSIANKDYGYGGVLCIDMG 360

Query: 361 TEERANSLMNILQNHTHFGLMAVSLGFFETLMSCSGSSTSSELNGEEKELAGISPGLIRM 420
           TEERAN LMN+LQNHTHFGLMAVSLGFFETLMSCSGSSTSSELNGEEKELAGISPGLIRM
Sbjct: 361 TEERANRLMNLLQNHTHFGLMAVSLGFFETLMSCSGSSTSSELNGEEKELAGISPGLIRM 420

Query: 421 SVGYFGTLEQKWSQLEKALLKMKI 440
           SVGY GTLEQKW+QLEKALLKMKI
Sbjct: 421 SVGYVGTLEQKWNQLEKALLKMKI 442

BLAST of Cla97C01G001520 vs. ExPASy TrEMBL
Match: A0A5A7TIP4 (Methionine gamma-lyase-like OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold376G001500 PE=3 SV=1)

HSP 1 Score: 786.9 bits (2031), Expect = 4.2e-224
Identity = 403/444 (90.77%), Postives = 417/444 (93.92%), Query Frame = 0

Query: 1   MIT-EVKAHNTSLPCWNNNNDKFSYDT-----TLAPSWPMREEDPVAALAMARPEFGEHG 60
           MIT +VKAH+TSLPC NNN   FSYDT     T   SWPM + DP AALAMARPEFGEHG
Sbjct: 1   MITDQVKAHDTSLPCKNNN---FSYDTKPTSITRLTSWPMGKVDPAAALAMARPEFGEHG 60

Query: 61  GVNLSIENSITFTVMEAETMRKMFEGELGPERDFFIYSRHFHPTVMALSRQMAALEGTEA 120
           GVN+SIENSITFTVMEAETMR+MFEGELGPERDFFIYSRHF+PTVMALSRQMAALEGTEA
Sbjct: 61  GVNMSIENSITFTVMEAETMRRMFEGELGPERDFFIYSRHFNPTVMALSRQMAALEGTEA 120

Query: 121 AYCTSSGMAAISSVLLQLVNSGGHIVASRTLYGGTHALLSHFLPRVCNITTTFVDVKEHE 180
           AYCTSSGMAA+SSVLLQLV+SGGHIVASRTLYGGTHALLSHFLPRV  ITTTFVDV+ HE
Sbjct: 121 AYCTSSGMAAVSSVLLQLVSSGGHIVASRTLYGGTHALLSHFLPRVSKITTTFVDVRNHE 180

Query: 181 MVRDAMVEGKTKVLYFETVANPTLTVANVPALSRMAHKKGVTVVVDNTFSPMIISPVRLG 240
           MVRDAMVEGKTKVLYFETVANPTLTVAN+P LSRMAH+KGVTVVVDNTFSPMIISP RLG
Sbjct: 181 MVRDAMVEGKTKVLYFETVANPTLTVANIPELSRMAHEKGVTVVVDNTFSPMIISPARLG 240

Query: 241 ADIVVHSCSKFISGGADIIAGAVCGSKSLVNSMMDLHQGTLMLLGPTMNAKVASELSQRI 300
           AD+VVHSCSKFISGGADIIAGAVCGSK LVNSMMDLHQGTLMLLGPTMNAKVASELSQRI
Sbjct: 241 ADVVVHSCSKFISGGADIIAGAVCGSKDLVNSMMDLHQGTLMLLGPTMNAKVASELSQRI 300

Query: 301 PHLSLRMKEHSHRALVFAQRMKNLGLKVIYPGLNDHPDHHLLSSIANKDYGYGGVLCIDM 360
           PHL LRMKEHS+RALVFAQRMKNLGLKVIYPGLNDHPDH LL SIANKDYGYGGVLCIDM
Sbjct: 301 PHLGLRMKEHSNRALVFAQRMKNLGLKVIYPGLNDHPDHQLLGSIANKDYGYGGVLCIDM 360

Query: 361 GTEERANSLMNILQNHTHFGLMAVSLGFFETLMSCSGSSTSSELNGEEKELAGISPGLIR 420
           GTEERAN LMN+LQNHTHFGLMAVSLGFFETLMSCSGSSTSSELNGEEKELAGISPGLIR
Sbjct: 361 GTEERANRLMNLLQNHTHFGLMAVSLGFFETLMSCSGSSTSSELNGEEKELAGISPGLIR 420

Query: 421 MSVGYFGTLEQKWSQLEKALLKMK 439
           MSVGY GTLEQKWSQLEKALLKMK
Sbjct: 421 MSVGYVGTLEQKWSQLEKALLKMK 441

BLAST of Cla97C01G001520 vs. ExPASy TrEMBL
Match: A0A1S3AU15 (methionine gamma-lyase-like OS=Cucumis melo OX=3656 GN=LOC103482860 PE=3 SV=1)

HSP 1 Score: 786.9 bits (2031), Expect = 4.2e-224
Identity = 403/444 (90.77%), Postives = 417/444 (93.92%), Query Frame = 0

Query: 1   MIT-EVKAHNTSLPCWNNNNDKFSYDT-----TLAPSWPMREEDPVAALAMARPEFGEHG 60
           MIT +VKAH+TSLPC NNN   FSYDT     T   SWPM + DP AALAMARPEFGEHG
Sbjct: 1   MITDQVKAHDTSLPCKNNN---FSYDTKPTSITRLTSWPMGKVDPAAALAMARPEFGEHG 60

Query: 61  GVNLSIENSITFTVMEAETMRKMFEGELGPERDFFIYSRHFHPTVMALSRQMAALEGTEA 120
           GVN+SIENSITFTVMEAETMR+MFEGELGPERDFFIYSRHF+PTVMALSRQMAALEGTEA
Sbjct: 61  GVNMSIENSITFTVMEAETMRRMFEGELGPERDFFIYSRHFNPTVMALSRQMAALEGTEA 120

Query: 121 AYCTSSGMAAISSVLLQLVNSGGHIVASRTLYGGTHALLSHFLPRVCNITTTFVDVKEHE 180
           AYCTSSGMAA+SSVLLQLV+SGGHIVASRTLYGGTHALLSHFLPRV  ITTTFVDV+ HE
Sbjct: 121 AYCTSSGMAAVSSVLLQLVSSGGHIVASRTLYGGTHALLSHFLPRVSKITTTFVDVRNHE 180

Query: 181 MVRDAMVEGKTKVLYFETVANPTLTVANVPALSRMAHKKGVTVVVDNTFSPMIISPVRLG 240
           MVRDAMVEGKTKVLYFETVANPTLTVAN+P LSRMAH+KGVTVVVDNTFSPMIISP RLG
Sbjct: 181 MVRDAMVEGKTKVLYFETVANPTLTVANIPELSRMAHEKGVTVVVDNTFSPMIISPARLG 240

Query: 241 ADIVVHSCSKFISGGADIIAGAVCGSKSLVNSMMDLHQGTLMLLGPTMNAKVASELSQRI 300
           AD+VVHSCSKFISGGADIIAGAVCGSK LVNSMMDLHQGTLMLLGPTMNAKVASELSQRI
Sbjct: 241 ADVVVHSCSKFISGGADIIAGAVCGSKDLVNSMMDLHQGTLMLLGPTMNAKVASELSQRI 300

Query: 301 PHLSLRMKEHSHRALVFAQRMKNLGLKVIYPGLNDHPDHHLLSSIANKDYGYGGVLCIDM 360
           PHL LRMKEHS+RALVFAQRMKNLGLKVIYPGLNDHPDH LL SIANKDYGYGGVLCIDM
Sbjct: 301 PHLGLRMKEHSNRALVFAQRMKNLGLKVIYPGLNDHPDHQLLGSIANKDYGYGGVLCIDM 360

Query: 361 GTEERANSLMNILQNHTHFGLMAVSLGFFETLMSCSGSSTSSELNGEEKELAGISPGLIR 420
           GTEERAN LMN+LQNHTHFGLMAVSLGFFETLMSCSGSSTSSELNGEEKELAGISPGLIR
Sbjct: 361 GTEERANRLMNLLQNHTHFGLMAVSLGFFETLMSCSGSSTSSELNGEEKELAGISPGLIR 420

Query: 421 MSVGYFGTLEQKWSQLEKALLKMK 439
           MSVGY GTLEQKWSQLEKALLKMK
Sbjct: 421 MSVGYVGTLEQKWSQLEKALLKMK 441

BLAST of Cla97C01G001520 vs. ExPASy TrEMBL
Match: A0A6J1I2P5 (methionine gamma-lyase-like OS=Cucurbita maxima OX=3661 GN=LOC111469051 PE=3 SV=1)

HSP 1 Score: 739.2 bits (1907), Expect = 1.0e-209
Identity = 372/429 (86.71%), Postives = 395/429 (92.07%), Query Frame = 0

Query: 15  WNNNNDKFSYDTTLAP---SW-PMREEDPVAALAMARPEFGEHGGVNLSIENSITFTVME 74
           W+  N K      L P   +W P  EEDP A+LA  RPEFGEHGGVNLSIENSI+FTVME
Sbjct: 3   WDVKNKKLKPTARLPPLPVTWRPEEEEDPAASLAAVRPEFGEHGGVNLSIENSISFTVME 62

Query: 75  AETMRKMFEGELGPERDFFIYSRHFHPTVMALSRQMAALEGTEAAYCTSSGMAAISSVLL 134
           AETMRKMFEGELGPE+D FIYSRHF+PTVMAL RQMAALEGTEAAYCTSSGMAA+SSVLL
Sbjct: 63  AETMRKMFEGELGPEQDCFIYSRHFNPTVMALGRQMAALEGTEAAYCTSSGMAAVSSVLL 122

Query: 135 QLVNSGGHIVASRTLYGGTHALLSHFLPRVCNITTTFVDVKEHEMVRDAMVEGKTKVLYF 194
           QL++SGGHIVASRTLYGGTHALLSHFLPRVCNITT+FVDVK+H MVRDAMV+GKTKVLYF
Sbjct: 123 QLLSSGGHIVASRTLYGGTHALLSHFLPRVCNITTSFVDVKDHAMVRDAMVKGKTKVLYF 182

Query: 195 ETVANPTLTVANVPALSRMAHKKGVTVVVDNTFSPMIISPVRLGADIVVHSCSKFISGGA 254
           ETVANPTLTVAN+P LSRMAH+KGVTVVVDNTFSPMIISP RLGAD+VVHSCSKFISGGA
Sbjct: 183 ETVANPTLTVANIPELSRMAHEKGVTVVVDNTFSPMIISPTRLGADVVVHSCSKFISGGA 242

Query: 255 DIIAGAVCGSKSLVNSMMDLHQGTLMLLGPTMNAKVASELSQRIPHLSLRMKEHSHRALV 314
           DIIAGAVCG K LVNSMMDLHQGTLMLLGPTMNAKVASELSQR+PHL+LRMKEHSHR LV
Sbjct: 243 DIIAGAVCGKKKLVNSMMDLHQGTLMLLGPTMNAKVASELSQRVPHLALRMKEHSHRTLV 302

Query: 315 FAQRMKNLGLKVIYPGLNDHPDHHLLSSIANKDYGYGGVLCIDMGTEERANSLMNILQNH 374
           FAQRMKNLGLKVIYPGL DHPDHHLLSSIANKDYGYGGVLCIDMGTEERAN LMN+LQNH
Sbjct: 303 FAQRMKNLGLKVIYPGLKDHPDHHLLSSIANKDYGYGGVLCIDMGTEERANRLMNLLQNH 362

Query: 375 THFGLMAVSLGFFETLMSCSGSSTSSELNGEEKELAGISPGLIRMSVGYFGTLEQKWSQL 434
           THFG +AVSLG+FETL+SCSGSSTSSE+N EEKELAGISPGLIRMSV Y GTLEQKWSQL
Sbjct: 363 THFGFLAVSLGYFETLISCSGSSTSSEMNVEEKELAGISPGLIRMSVAYVGTLEQKWSQL 422

Query: 435 EKALLKMKI 440
           EKALLK+ I
Sbjct: 423 EKALLKLNI 431

BLAST of Cla97C01G001520 vs. ExPASy TrEMBL
Match: A0A6J1E0P6 (methionine gamma-lyase OS=Momordica charantia OX=3673 GN=LOC111026086 PE=3 SV=1)

HSP 1 Score: 706.1 bits (1821), Expect = 9.4e-200
Identity = 356/443 (80.36%), Postives = 391/443 (88.26%), Query Frame = 0

Query: 2   ITEVKAHNTSLPCWNNNNDKFSYDTTLAPSWPMR---EEDPVAALAMARPEFGEHGGVNL 61
           + EV+A     PCWN       Y+   +P    +   EEDP AALA+A  EFGEHGGVN+
Sbjct: 1   MAEVEARPIPHPCWNEKKKLIKYEKAKSPKPATKSATEEDPAAALALAEHEFGEHGGVNM 60

Query: 62  SIENSITFTVMEAETMRKMFEGELGPERDFFIYSRHFHPTVMALSRQMAALEGTEAAYCT 121
           S+E SITFTVMEAETMR+MFEGE GPE + FIYSRHF+PTVMALSRQMAALEGTEAAYCT
Sbjct: 61  SVEASITFTVMEAETMRRMFEGEEGPEGECFIYSRHFNPTVMALSRQMAALEGTEAAYCT 120

Query: 122 SSGMAAISSVLLQLVNSGGHIVASRTLYGGTHALLSHFLPRVCNITTTFVDVKEHEMVRD 181
           SSGMAA+SSVLLQLV+SGGHIVASRTLYGGTHALLSHFLPRVCNITTTFVD+K+H MV +
Sbjct: 121 SSGMAAVSSVLLQLVSSGGHIVASRTLYGGTHALLSHFLPRVCNITTTFVDIKDHGMVAN 180

Query: 182 AMVEGKTKVLYFETVANPTLTVANVPALSRMAHKKGVTVVVDNTFSPMIISPVRLGADIV 241
           AM+EGKTKVLYFETVANPTLTVA++P LSRMAH+KGVTVVVDNTF+PM+ISPVRLGAD+V
Sbjct: 181 AMLEGKTKVLYFETVANPTLTVADIPELSRMAHEKGVTVVVDNTFAPMVISPVRLGADVV 240

Query: 242 VHSCSKFISGGADIIAGAVCGSKSLVNSMMDLHQGTLMLLGPTMNAKVASELSQRIPHLS 301
           VHS SKFISGGADIIAGAVCGSK LV+SMMDL QGTLMLLGPTMN KVA ELSQR+PHL 
Sbjct: 241 VHSSSKFISGGADIIAGAVCGSKKLVHSMMDLQQGTLMLLGPTMNPKVACELSQRVPHLG 300

Query: 302 LRMKEHSHRALVFAQRMKNLGLKVIYPGLNDHPDHHLLSSIANKD--YGYGGVLCIDMGT 361
           LRMKEH HRAL+FAQR++NLG KVIYPGL DHPDHHL++SIAN    YGYGGVLCIDM T
Sbjct: 301 LRMKEHCHRALIFAQRIQNLGFKVIYPGLKDHPDHHLINSIANTSYGYGYGGVLCIDMET 360

Query: 362 EERANSLMNILQNHTHFGLMAVSLGFFETLMSCSGSSTSSELNGEEKELAGISPGLIRMS 421
           EERAN LMN+LQNHT FGLMAVSLGFFETLMSCSGSSTSSELN EEKELAGISPGL+R+S
Sbjct: 361 EERANRLMNLLQNHTKFGLMAVSLGFFETLMSCSGSSTSSELNAEEKELAGISPGLVRIS 420

Query: 422 VGYFGTLEQKWSQLEKALLKMKI 440
           VGY GTLEQKWSQLE+ LLKMK+
Sbjct: 421 VGYVGTLEQKWSQLERGLLKMKL 443

BLAST of Cla97C01G001520 vs. TAIR 10
Match: AT1G64660.1 (methionine gamma-lyase )

HSP 1 Score: 614.8 bits (1584), Expect = 5.5e-176
Identity = 299/402 (74.38%), Postives = 352/402 (87.56%), Query Frame = 0

Query: 36  EEDPVAALAMARPEFGEHGGVNLSIENSITFTVMEAETMRKMFEGELGPERDFFIYSRHF 95
           + DP AA+A  R EFGEHGGVN+SIE S TFTVME +TMR+MF GELGP+ DF++YSRHF
Sbjct: 40  DADPAAAIANIRHEFGEHGGVNMSIEASATFTVMEPDTMRRMFTGELGPDNDFYVYSRHF 99

Query: 96  HPTVMALSRQMAALEGTEAAYCTSSGMAAISSVLLQLVNSGGHIVASRTLYGGTHALLSH 155
           +PTV+ LSRQMAALEGT+AAYCTSSGM+AISSV+LQL +SGGH+VA+ TLYGGTHALLSH
Sbjct: 100 NPTVLNLSRQMAALEGTQAAYCTSSGMSAISSVMLQLCSSGGHVVAASTLYGGTHALLSH 159

Query: 156 FLPRVCNITTTFVDVKEHEMVRDAMVEGKTKVLYFETVANPTLTVANVPALSRMAHKKGV 215
           FLPR CNITT+FVD+ +H  V +A+VEG+T+VLYFE+VANPTLTVA++P LSRMAH+KGV
Sbjct: 160 FLPRTCNITTSFVDITDHGAVANAIVEGRTQVLYFESVANPTLTVADIPELSRMAHEKGV 219

Query: 216 TVVVDNTFSPMIISPVRLGADIVVHSCSKFISGGADIIAGAVCGSKSLVNSMMDLHQGTL 275
           TVVVDNTF+PM++SP +LGAD+VVHS SKFISGGADIIAGAVCGS++LV  MMDL  G+L
Sbjct: 220 TVVVDNTFAPMVLSPAKLGADVVVHSISKFISGGADIIAGAVCGSENLVKEMMDLRGGSL 279

Query: 276 MLLGPTMNAKVASELSQRIPHLSLRMKEHSHRALVFAQRMKNLGLKVIYPGLNDHPDHHL 335
           MLLGPTMNAKVA ELS+RIPHL LRM+EHSHRA V+A+RM++LG+KVIYPGL  HP H L
Sbjct: 280 MLLGPTMNAKVAFELSERIPHLGLRMREHSHRAQVYAERMRDLGMKVIYPGLETHPQHKL 339

Query: 336 LSSIANKDYGYGGVLCIDMGTEERANSLMNILQNHTHFGLMAVSLGFFETLMSCSGSSTS 395
              + N+DYGYGG+L IDM TEE+AN LM  LQN T FG MAVSLG++ETLMSCSGSSTS
Sbjct: 340 FKGMVNRDYGYGGLLSIDMETEEKANKLMAYLQNATQFGFMAVSLGYYETLMSCSGSSTS 399

Query: 396 SELNGEEKELAGISPGLIRMSVGYFGTLEQKWSQLEKALLKM 438
           SEL+  +KE AGISPGL+RMSVGY GTLEQKW+Q EKA L+M
Sbjct: 400 SELDPSQKEAAGISPGLVRMSVGYVGTLEQKWTQFEKAFLRM 441

BLAST of Cla97C01G001520 vs. TAIR 10
Match: AT3G01120.1 (Pyridoxal phosphate (PLP)-dependent transferases superfamily protein )

HSP 1 Score: 161.4 bits (407), Expect = 1.7e-39
Identity = 109/334 (32.63%), Postives = 180/334 (53.89%), Query Frame = 0

Query: 89  FIYSRHFHPTVMALSRQMAALEGTEAAYCTSSGMAAISSVLLQLVNSGGHIVASRTLYGG 148
           F Y R+ +PT + L  +++ALEG E+    +SGM A + +LL LV +GGHIV +   Y  
Sbjct: 224 FEYGRYGNPTTVVLEDKISALEGAESTLVMASGMCASTVMLLALVPAGGHIVTTTDCYRK 283

Query: 149 THALLSHFLPRVCNITTTFVDVKEHEMVRDAMVEGKTKVLYFETVANPTLTVANVPALSR 208
           T   + +FLP++  IT T +D  +   +  A+ E K  + + E+  NP L   ++  +S+
Sbjct: 284 TRIFMENFLPKL-GITVTVIDPADIAGLEAAVNEFKVSLFFTESPTNPFLRCVDIELVSK 343

Query: 209 MAHKKGVTVVVDNTF-SPMIISPVRLGADIVVHSCSKFISGGADIIAGAVCGSKSLVNSM 268
           + HK+G  V +D TF +P+    + LGAD+VVHS +K+I G  D++AG +CGS  LV+ +
Sbjct: 344 ICHKRGTLVCIDGTFATPLNQKALALGADLVVHSATKYIGGHNDVLAGCICGSLKLVSEI 403

Query: 269 MDLHQGTLMLLGPTMNAKVASELSQRIPHLSLRMKEHSHRALVFAQRMK--NLGLKVIYP 328
            +LH     +LG T+N   A  + + +  L LR+++ +  A   A+ ++       V YP
Sbjct: 404 RNLHH----VLGGTLNPNAAYLIIRGMKTLHLRVQQQNSTAFRMAEILEAHPKVSHVYYP 463

Query: 329 GLNDHPDHHLLSSIANKDYGYGGVLCIDM-GTEERANSLMNILQNHTHFGLMAVSLGFFE 388
           GL  HP+H L      +  G+GGV+  ++ G  E     ++ L+       +A S G  E
Sbjct: 464 GLPSHPEHELAK---RQMTGFGGVVSFEIDGDIETTIKFVDSLK----IPYIAPSFGGCE 523

Query: 389 TLMSCSGSSTSSELNGEEKELAGISPGLIRMSVG 419
           +++      +  +L  EE+   GI   L+R S G
Sbjct: 524 SIVDQPAIMSYWDLPQEERLKYGIKDNLVRFSFG 545

BLAST of Cla97C01G001520 vs. TAIR 10
Match: AT3G57050.1 (cystathionine beta-lyase )

HSP 1 Score: 141.4 bits (355), Expect = 1.8e-33
Identity = 117/363 (32.23%), Postives = 188/363 (51.79%), Query Frame = 0

Query: 62  NSITFTVMEAETMRKMFEGELGPERDFFIYSRHFHPTVMALSRQMAALEGTEAAYCTSSG 121
           ++++  + +  T ++    E GP    + Y+R  +PT  AL   +A L+  + A+C +SG
Sbjct: 102 DAMSTPLYQTATFKQPSAIENGP----YDYTRSGNPTRDALESLLAKLDKADRAFCFTSG 161

Query: 122 MAAISSVLLQLVNSGGHIVASRTLYGGTHALLSHFLPRVCNITTTFVDVKEHEMVRDAMV 181
           MAA+S+V   L+ +G  IVA   +YGG+  LLS  +PR   +       K  E+   A +
Sbjct: 162 MAALSAV-THLIKNGEEIVAGDDVYGGSDRLLSQVVPRSGVVVKRVNTTKLDEVA--AAI 221

Query: 182 EGKTKVLYFETVANPTLTVANVPALSRMAHKKGVTVVVDNT-FSPMIISPVRLGADIVVH 241
             +TK+++ E+  NP   ++++  +S MAH +G  V+VDN+  SP++  P+ LGADIV+H
Sbjct: 222 GPQTKLVWLESPTNPRQQISDIRKISEMAHAQGALVLVDNSIMSPVLSRPLELGADIVMH 281

Query: 242 SCSKFISGGADIIAG--AVCGSKSLVNSMMDLHQGTLMLLGPTMNAKVASELSQR-IPHL 301
           S +KFI+G +D++AG  AV G K L   +  L       L P         L  R I  +
Sbjct: 282 SATKFIAGHSDVMAGVLAVKGEK-LAKEVYFLQNSEGSGLAP-----FDCWLCLRGIKTM 341

Query: 302 SLRMKEHSHRALVFAQRMKN--LGLKVIYPGLNDHPDHHLLSSIANKDYGYGGVLCIDMG 361
           +LR+++    A   A  + +     KV Y GL DHP HHL  S A    G G V     G
Sbjct: 342 ALRIEKQQENARKIAMYLSSHPRVKKVYYAGLPDHPGHHLHFSQAK---GAGSVFSFITG 401

Query: 362 TEERANSLMNILQNHTHFGLMAVSLGFFETLMSCSGSSTSSELNGEEKELAGISPGLIRM 419
           +     +L   L   T +  +AVS G  ++L+S     + + +  E +E  G++  L+R+
Sbjct: 402 SV----ALSKHLVETTKYFSIAVSFGSVKSLISMPCFMSHASIPAEVREARGLTEDLVRI 444

BLAST of Cla97C01G001520 vs. TAIR 10
Match: AT3G57050.2 (cystathionine beta-lyase )

HSP 1 Score: 141.4 bits (355), Expect = 1.8e-33
Identity = 117/363 (32.23%), Postives = 188/363 (51.79%), Query Frame = 0

Query: 62  NSITFTVMEAETMRKMFEGELGPERDFFIYSRHFHPTVMALSRQMAALEGTEAAYCTSSG 121
           ++++  + +  T ++    E GP    + Y+R  +PT  AL   +A L+  + A+C +SG
Sbjct: 87  DAMSTPLYQTATFKQPSAIENGP----YDYTRSGNPTRDALESLLAKLDKADRAFCFTSG 146

Query: 122 MAAISSVLLQLVNSGGHIVASRTLYGGTHALLSHFLPRVCNITTTFVDVKEHEMVRDAMV 181
           MAA+S+V   L+ +G  IVA   +YGG+  LLS  +PR   +       K  E+   A +
Sbjct: 147 MAALSAV-THLIKNGEEIVAGDDVYGGSDRLLSQVVPRSGVVVKRVNTTKLDEVA--AAI 206

Query: 182 EGKTKVLYFETVANPTLTVANVPALSRMAHKKGVTVVVDNT-FSPMIISPVRLGADIVVH 241
             +TK+++ E+  NP   ++++  +S MAH +G  V+VDN+  SP++  P+ LGADIV+H
Sbjct: 207 GPQTKLVWLESPTNPRQQISDIRKISEMAHAQGALVLVDNSIMSPVLSRPLELGADIVMH 266

Query: 242 SCSKFISGGADIIAG--AVCGSKSLVNSMMDLHQGTLMLLGPTMNAKVASELSQR-IPHL 301
           S +KFI+G +D++AG  AV G K L   +  L       L P         L  R I  +
Sbjct: 267 SATKFIAGHSDVMAGVLAVKGEK-LAKEVYFLQNSEGSGLAP-----FDCWLCLRGIKTM 326

Query: 302 SLRMKEHSHRALVFAQRMKN--LGLKVIYPGLNDHPDHHLLSSIANKDYGYGGVLCIDMG 361
           +LR+++    A   A  + +     KV Y GL DHP HHL  S A    G G V     G
Sbjct: 327 ALRIEKQQENARKIAMYLSSHPRVKKVYYAGLPDHPGHHLHFSQAK---GAGSVFSFITG 386

Query: 362 TEERANSLMNILQNHTHFGLMAVSLGFFETLMSCSGSSTSSELNGEEKELAGISPGLIRM 419
           +     +L   L   T +  +AVS G  ++L+S     + + +  E +E  G++  L+R+
Sbjct: 387 SV----ALSKHLVETTKYFSIAVSFGSVKSLISMPCFMSHASIPAEVREARGLTEDLVRI 429

BLAST of Cla97C01G001520 vs. TAIR 10
Match: AT1G33320.1 (Pyridoxal phosphate (PLP)-dependent transferases superfamily protein )

HSP 1 Score: 139.4 bits (350), Expect = 6.8e-33
Identity = 99/336 (29.46%), Postives = 178/336 (52.98%), Query Frame = 0

Query: 87  DFFIYSRHFHPTVMALSRQMAALEGTEAAYCTSSGMAAISSVLLQLVNSGGHIVASRTLY 146
           D + Y+R+ +PT MAL  +++ LEG E+    +SGM A + +LL LV + GHIVA++  Y
Sbjct: 72  DLYEYARYGNPTTMALEEKISVLEGAESTLVMASGMYASNVMLLALVPTNGHIVATKDCY 131

Query: 147 GGTHALLSHFLPRVCNITTTFVDVKEHEMVRDAMVEGKTKVLYFETVANPTLTVANVPAL 206
             T   + +FL ++  IT TF+D  +   ++  +   +  + + E+  NP L   ++  +
Sbjct: 132 KETRIFMENFLTKL-GITVTFIDSDDIAGLQTLVNNHEVSLFFTESPTNPFLRCVDIKLV 191

Query: 207 SRMAHKKGVTVVVDNTF-SPMIISPVRLGADIVVHSCSKFISGGADIIAGAVCGSKSLVN 266
           S++ H++G  V +D T  +P+    + LGAD+V HS +K+I G  D +AG++ GS  LV+
Sbjct: 192 SKICHRRGTLVCIDATIATPINQKTLALGADLVHHSATKYIGGHNDFLAGSISGSMELVS 251

Query: 267 SMMDLHQGTLMLLGPTMNAKVASELSQRIPHLSLRMKEHSHRALVFAQRMK--NLGLKVI 326
            + +LH+    LLG T+N   A  L + +  + LR+++ +   +  AQ ++      +V 
Sbjct: 252 KIRNLHK----LLGGTLNPNAAYLLIRGMKTMHLRVRQQNSTGMKMAQVLEAHPKVSRVY 311

Query: 327 YPGLNDHPDHHLLSSIANKDYGYGGVLCIDM-GTEERANSLMNILQNHTHFGLMAVSLGF 386
           Y GL  HP+H +      +  G GG++  ++ G  +     ++ L+       +A S G 
Sbjct: 312 YLGLPSHPEHLIAK---RQMTGIGGLISFEIDGDLKTTIKFIDALK----IPYLAASFGG 371

Query: 387 FETLMSCSGSSTSSELNGEEKELAGISPGLIRMSVG 419
            E+L+    +    ++  EE+   G    L+R S G
Sbjct: 372 CESLVDQLATGI-WDIPREERLKDGFQDNLVRFSFG 394

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
XP_038875936.11.5e-22890.97methionine gamma-lyase-like [Benincasa hispida][more]
XP_004143943.39.3e-22690.99methionine gamma-lyase [Cucumis sativus] >KGN49981.1 hypothetical protein Csa_00... [more]
XP_008437446.18.7e-22490.77PREDICTED: methionine gamma-lyase-like [Cucumis melo] >KAA0042648.1 methionine g... [more]
KAG7017043.14.5e-21288.11Methionine gamma-lyase, partial [Cucurbita argyrosperma subsp. argyrosperma][more]
KAG6579584.11.1e-21087.88Methionine gamma-lyase, partial [Cucurbita argyrosperma subsp. sororia][more]
Match NameE-valueIdentityDescription
Q9SGU97.7e-17574.38Methionine gamma-lyase OS=Arabidopsis thaliana OX=3702 GN=MGL PE=1 SV=1[more]
Q8L0X43.0e-4937.24L-methionine gamma-lyase OS=Fusobacterium nucleatum subsp. polymorphum OX=76857 ... [more]
Q8RDT43.6e-4736.06L-methionine gamma-lyase OS=Fusobacterium nucleatum subsp. nucleatum (strain ATC... [more]
Q7MX715.2e-4637.81L-methionine gamma-lyase OS=Porphyromonas gingivalis (strain ATCC BAA-308 / W83)... [more]
O053941.7e-4434.50Cystathionine gamma-lyase OS=Bacillus subtilis (strain 168) OX=224308 GN=mccB PE... [more]
Match NameE-valueIdentityDescription
A0A0A0KQL74.5e-22690.99Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_5G148520 PE=3 SV=1[more]
A0A5A7TIP44.2e-22490.77Methionine gamma-lyase-like OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaf... [more]
A0A1S3AU154.2e-22490.77methionine gamma-lyase-like OS=Cucumis melo OX=3656 GN=LOC103482860 PE=3 SV=1[more]
A0A6J1I2P51.0e-20986.71methionine gamma-lyase-like OS=Cucurbita maxima OX=3661 GN=LOC111469051 PE=3 SV=... [more]
A0A6J1E0P69.4e-20080.36methionine gamma-lyase OS=Momordica charantia OX=3673 GN=LOC111026086 PE=3 SV=1[more]
Match NameE-valueIdentityDescription
AT1G64660.15.5e-17674.38methionine gamma-lyase [more]
AT3G01120.11.7e-3932.63Pyridoxal phosphate (PLP)-dependent transferases superfamily protein [more]
AT3G57050.11.8e-3332.23cystathionine beta-lyase [more]
AT3G57050.21.8e-3332.23cystathionine beta-lyase [more]
AT1G33320.16.8e-3329.46Pyridoxal phosphate (PLP)-dependent transferases superfamily protein [more]
InterPro
Analysis Name: InterPro Annotations of Watermelon (97103) v2.5
Date Performed: 2022-01-31
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR000277Cys/Met metabolism, pyridoxal phosphate-dependent enzymePIRSFPIRSF001434CGScoord: 31..438
e-value: 1.2E-100
score: 335.0
IPR000277Cys/Met metabolism, pyridoxal phosphate-dependent enzymePFAMPF01053Cys_Met_Meta_PPcoord: 52..418
e-value: 3.0E-82
score: 276.2
IPR000277Cys/Met metabolism, pyridoxal phosphate-dependent enzymePANTHERPTHR11808TRANS-SULFURATION ENZYME FAMILY MEMBERcoord: 21..438
IPR015421Pyridoxal phosphate-dependent transferase, major domainGENE3D3.40.640.10coord: 41..294
e-value: 1.5E-66
score: 226.2
IPR015422Pyridoxal phosphate-dependent transferase, small domainGENE3D3.90.1150.10Aspartate Aminotransferase, domain 1coord: 299..439
e-value: 1.7E-23
score: 85.1
NoneNo IPR availablePIRSRPIRSR001434-2PIRSR001434-2coord: 92..398
e-value: 5.7E-78
score: 259.9
NoneNo IPR availablePANTHERPTHR11808:SF76METHIONINE GAMMA-LYASE-LIKEcoord: 21..438
IPR015424Pyridoxal phosphate-dependent transferaseSUPERFAMILY53383PLP-dependent transferasescoord: 52..425

Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
Cla97C01G001520.1Cla97C01G001520.1mRNA


GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
biological_process GO:0019343 cysteine biosynthetic process via cystathionine
biological_process GO:0019346 transsulfuration
cellular_component GO:0005737 cytoplasm
molecular_function GO:0004123 cystathionine gamma-lyase activity
molecular_function GO:0018826 methionine gamma-lyase activity
molecular_function GO:0030170 pyridoxal phosphate binding
molecular_function GO:0003824 catalytic activity