ClCG10G011490 (gene) Watermelon (Charleston Gray) v2.5

Overview
NameClCG10G011490
Typegene
OrganismCitrullus lanatus subsp. vulgaris cv. Charleston Gray (Watermelon (Charleston Gray) v2.5)
DescriptionPMEI domain-containing protein
LocationCG_Chr10: 25166726 .. 25167565 (-)
RNA-Seq ExpressionClCG10G011490
SyntenyClCG10G011490
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: exonCDSpolypeptide
Hold the cursor over a type above to highlight its positions in the sequence below.
ATGGAGCTCATCAATAATGTCTTCCTCCTCCTCGCCCTCTCTTCTCTTTTCACCTCTGCCATTGCCGTTTGCGTCCCTCGCAATACCTTTAACTTCGGATCGAATTCTATTCCACCAGTCTTGTCGCCGCAAATAGCCCCACAGTCACTGCCAGTTACGGTGTCGCCGCCACAATCTACCCCATCACAGGCACCGTCGGTTACGGTTTCTCCGCCACAATCTCCCCCATCACAGGCACCGCCGGCTACGGTTTCTCCACCACAATCTTCCACAGCACCGACGAATTCCGCTCCGGCGGAGGAGGAATCCGGCTCTGAAGAACACTCAGGACTACCTGTATTTCACAAATTACATTCGGATGATTTCGTATCGTCGGTCACATCGAGTGTGACGACGGATCTGGCGGAGATCTGCGATGTGACGAGCAATCCTCAGCTATGTAGAACCTCGATTGCATCTCACATCAATGGACCGACGGTGGATCCTACTACGGCACTGAAAACAGAAATTGAACAATCGATTAAAGAGGTTTCGAAAGCAATTGCAACATTGAACACACTTCGTAAAGATTCAGCAGCATCGAATGCGGAGATCGCGTGTTACGATACTTGCTTGGAGAATTTCGATATGGCTATTGAAGACCTAAAAGCTGGATTGGAATCGATCGATGTCCACGACAATGGTAGAATGGAGAGCGTGCTTACGGCCGTATTGACAGATCTGATGACTTGCGATGATACATTTGCAGAGATGGGAGTGGATTCTCCATTGGATAGTCTTAGCGCGAAGATGAGCAAGTACGCCAGCAATTGCTTGGCGATTTCGAAATTGCTTCTGTAA

mRNA sequence

ATGGAGCTCATCAATAATGTCTTCCTCCTCCTCGCCCTCTCTTCTCTTTTCACCTCTGCCATTGCCGTTTGCGTCCCTCGCAATACCTTTAACTTCGGATCGAATTCTATTCCACCAGTCTTGTCGCCGCAAATAGCCCCACAGTCACTGCCAGTTACGGTGTCGCCGCCACAATCTACCCCATCACAGGCACCGTCGGTTACGGTTTCTCCGCCACAATCTCCCCCATCACAGGCACCGCCGGCTACGGTTTCTCCACCACAATCTTCCACAGCACCGACGAATTCCGCTCCGGCGGAGGAGGAATCCGGCTCTGAAGAACACTCAGGACTACCTGTATTTCACAAATTACATTCGGATGATTTCGTATCGTCGGTCACATCGAGTGTGACGACGGATCTGGCGGAGATCTGCGATGTGACGAGCAATCCTCAGCTATGTAGAACCTCGATTGCATCTCACATCAATGGACCGACGGTGGATCCTACTACGGCACTGAAAACAGAAATTGAACAATCGATTAAAGAGGTTTCGAAAGCAATTGCAACATTGAACACACTTCGTAAAGATTCAGCAGCATCGAATGCGGAGATCGCGTGTTACGATACTTGCTTGGAGAATTTCGATATGGCTATTGAAGACCTAAAAGCTGGATTGGAATCGATCGATGTCCACGACAATGGTAGAATGGAGAGCGTGCTTACGGCCGTATTGACAGATCTGATGACTTGCGATGATACATTTGCAGAGATGGGAGTGGATTCTCCATTGGATAGTCTTAGCGCGAAGATGAGCAAGTACGCCAGCAATTGCTTGGCGATTTCGAAATTGCTTCTGTAA

Coding sequence (CDS)

ATGGAGCTCATCAATAATGTCTTCCTCCTCCTCGCCCTCTCTTCTCTTTTCACCTCTGCCATTGCCGTTTGCGTCCCTCGCAATACCTTTAACTTCGGATCGAATTCTATTCCACCAGTCTTGTCGCCGCAAATAGCCCCACAGTCACTGCCAGTTACGGTGTCGCCGCCACAATCTACCCCATCACAGGCACCGTCGGTTACGGTTTCTCCGCCACAATCTCCCCCATCACAGGCACCGCCGGCTACGGTTTCTCCACCACAATCTTCCACAGCACCGACGAATTCCGCTCCGGCGGAGGAGGAATCCGGCTCTGAAGAACACTCAGGACTACCTGTATTTCACAAATTACATTCGGATGATTTCGTATCGTCGGTCACATCGAGTGTGACGACGGATCTGGCGGAGATCTGCGATGTGACGAGCAATCCTCAGCTATGTAGAACCTCGATTGCATCTCACATCAATGGACCGACGGTGGATCCTACTACGGCACTGAAAACAGAAATTGAACAATCGATTAAAGAGGTTTCGAAAGCAATTGCAACATTGAACACACTTCGTAAAGATTCAGCAGCATCGAATGCGGAGATCGCGTGTTACGATACTTGCTTGGAGAATTTCGATATGGCTATTGAAGACCTAAAAGCTGGATTGGAATCGATCGATGTCCACGACAATGGTAGAATGGAGAGCGTGCTTACGGCCGTATTGACAGATCTGATGACTTGCGATGATACATTTGCAGAGATGGGAGTGGATTCTCCATTGGATAGTCTTAGCGCGAAGATGAGCAAGTACGCCAGCAATTGCTTGGCGATTTCGAAATTGCTTCTGTAA

Protein sequence

MELINNVFLLLALSSLFTSAIAVCVPRNTFNFGSNSIPPVLSPQIAPQSLPVTVSPPQSTPSQAPSVTVSPPQSPPSQAPPATVSPPQSSTAPTNSAPAEEESGSEEHSGLPVFHKLHSDDFVSSVTSSVTTDLAEICDVTSNPQLCRTSIASHINGPTVDPTTALKTEIEQSIKEVSKAIATLNTLRKDSAASNAEIACYDTCLENFDMAIEDLKAGLESIDVHDNGRMESVLTAVLTDLMTCDDTFAEMGVDSPLDSLSAKMSKYASNCLAISKLLL
Homology
BLAST of ClCG10G011490 vs. NCBI nr
Match: XP_038903716.1 (21 kDa protein-like [Benincasa hispida])

HSP 1 Score: 385.2 bits (988), Expect = 4.8e-103
Identity = 225/286 (78.67%), Postives = 243/286 (84.97%), Query Frame = 0

Query: 1   MELINNVFLLLALSSLFTSAIAVCVPRNTFNFGSNSIPPVLSPQIAPQSL-PVTVSPPQS 60
           MELINNVFLLLA SSLF+SAIA+CVPRN   F S SIPP + PQIAPQ+   VT S PQS
Sbjct: 1   MELINNVFLLLAFSSLFSSAIAICVPRNALGFVSTSIPPTIEPQIAPQTTESVTASLPQS 60

Query: 61  TPSQAP--SVTVSPPQSPPSQAPPA--TVSPPQSSTAPTNSAPAEE--ESGSEEHSGLPV 120
             +Q P   VTVSPPQSPP+QAPPA  TVSPPQS +  TNSAPAEE  ES SEE SGLP 
Sbjct: 61  PSAQTPQVEVTVSPPQSPPTQAPPATVTVSPPQSPSPSTNSAPAEEEQESASEEDSGLPE 120

Query: 121 FHKLHSDDFVSSVTSSVTTDLAEICDVTSNPQLCRTSIASHINGPTVDPTTALKTEIEQS 180
           F KL SD+ VSSV SS TTDL EICDVTSNPQLC+TSI+S I+G TVDPT+ALKTEI+QS
Sbjct: 121 FQKLPSDNLVSSVASSTTTDLVEICDVTSNPQLCKTSISSQIHGMTVDPTSALKTEIQQS 180

Query: 181 IKEVSKAIATLNTLRKDSAASNAEIACYDTCLENFDMAIEDLKAGLESIDVHDNGRMESV 240
           +KEV+KAIATL  LRKD +ASN EIACYDTCLENFD+AIEDLKAGLESIDVHD GRMESV
Sbjct: 181 MKEVTKAIATLKELRKDPSASNVEIACYDTCLENFDIAIEDLKAGLESIDVHDVGRMESV 240

Query: 241 LTAVLTDLMTCDDTFAEMGVDSPLDSLSAKMSKYASNCLAISKLLL 280
           LTAVLTDLMTCDDTFAEMGVDSPLDSLS +MSKYASNCLAISKLLL
Sbjct: 241 LTAVLTDLMTCDDTFAEMGVDSPLDSLSTRMSKYASNCLAISKLLL 286

BLAST of ClCG10G011490 vs. NCBI nr
Match: XP_011654315.2 (uncharacterized protein LOC105435336 [Cucumis sativus] >KAE8649055.1 hypothetical protein Csa_015367 [Cucumis sativus])

HSP 1 Score: 307.8 bits (787), Expect = 9.8e-80
Identity = 185/288 (64.24%), Postives = 218/288 (75.69%), Query Frame = 0

Query: 1   MELIN----NVFLLLALSSLFTSAIAVCVPRNTFNFGSNSIPPVLSPQIAPQSLPVTVSP 60
           MELIN    NVFLLLA SS+F+ A+AVCVPRNT + GS +IPP+    ++PQ        
Sbjct: 1   MELINNSIHNVFLLLAFSSVFSFAVAVCVPRNTSDIGSTAIPPLFEQTVSPQ------VA 60

Query: 61  PQSTPSQAPSVTVSPPQSPPSQAPPATVSPPQSSTAPTNSAPAEEESGSE-EHSGLPV-- 120
           PQ  P   P+VTVS  + P + +P  + S P S++    +A   EE+ SE E   L +  
Sbjct: 61  PQVAPQSPPAVTVS--EEPEAASPETSPSQPSSASESEEAASESEEAASETEEDDLGLLG 120

Query: 121 --FHKLHSDDFVSSVTSSVTTDLAEICDVTSNPQLCRTSIASHINGPTVDPTTALKTEIE 180
               +LHS D +SSVTSSVTT L +ICDVTSNPQLC+TSI+SHI G  VDP +ALKTEI+
Sbjct: 121 GGLDQLHSADLISSVTSSVTTSLVDICDVTSNPQLCKTSISSHIEGTKVDPASALKTEID 180

Query: 181 QSIKEVSKAIATLNTLRKDSAASNAEIACYDTCLENFDMAIEDLKAGLESIDVHDNGRME 240
           +SIKEV+KAIATLN+LRKDSAAS  EIACYDTCLENF+MAI+DLKAG+ESI+  D GRME
Sbjct: 181 ESIKEVAKAIATLNSLRKDSAASETEIACYDTCLENFEMAIDDLKAGVESINARDAGRME 240

Query: 241 SVLTAVLTDLMTCDDTFAEMGVDSPLDSLSAKMSKYASNCLAISKLLL 280
           SVLTAV+TDL TCDDTFAEMGVDSPLDSLS KMSKYASNCLAISKLLL
Sbjct: 241 SVLTAVMTDLTTCDDTFAEMGVDSPLDSLSTKMSKYASNCLAISKLLL 280

BLAST of ClCG10G011490 vs. NCBI nr
Match: XP_008454280.1 (PREDICTED: pectinesterase inhibitor 1 [Cucumis melo] >KAA0044419.1 pectinesterase inhibitor 1 [Cucumis melo var. makuwa] >TYK29546.1 pectinesterase inhibitor 1 [Cucumis melo var. makuwa])

HSP 1 Score: 297.7 bits (761), Expect = 1.0e-76
Identity = 179/281 (63.70%), Postives = 206/281 (73.31%), Query Frame = 0

Query: 4   INNVFLLLALSSLFTSAIAVCVPR--NTFNFGSNSIPPVLSPQIAPQSLPVTVSPPQSTP 63
           INNVFLLLA SS+F SAIA+CVP   N  +  S +IPP+L   ++PQ             
Sbjct: 8   INNVFLLLAFSSVFFSAIAICVPLNINITDVESTAIPPLLEQTVSPQ------------- 67

Query: 64  SQAPSVTVSPPQSPPSQAPPATVSPPQSSTAP---TNSAPAEEESGSEEHSGLPVFHKLH 123
                  V+P QSPP+     TVS P  +T+P   T+   +  E   EE+SGL     LH
Sbjct: 68  -------VAPDQSPPA---VTTVSQPPEATSPQTSTSDQSSSNEISDEENSGLLGLDYLH 127

Query: 124 SDDFVSSVTSSVTTDLAEICDVTSNPQLCRTSIASHINGPTVDPTTALKTEIEQSIKEVS 183
           SD  +S+  S+V T+L EICDVTSNPQLC+TSI+SHI G  VDP +ALKTEIE+SIKEV+
Sbjct: 128 SDGLISTAASTVKTNLVEICDVTSNPQLCKTSISSHIEGTKVDPASALKTEIEESIKEVT 187

Query: 184 KAIATLNTLRKDSAASNAEIACYDTCLENFDMAIEDLKAGLESIDVHDNGRMESVLTAVL 243
           KAIATLN+LRKDS+AS  EIACYDTCLENFDMAIEDLKAG+ESID  D GRMESVLTAVL
Sbjct: 188 KAIATLNSLRKDSSASETEIACYDTCLENFDMAIEDLKAGVESIDARDAGRMESVLTAVL 247

Query: 244 TDLMTCDDTFAEMGVDSPLDSLSAKMSKYASNCLAISKLLL 280
           TDL TCDDTFAEMGVDSPLD+LS KMSKYASNCLAISKLLL
Sbjct: 248 TDLTTCDDTFAEMGVDSPLDNLSTKMSKYASNCLAISKLLL 265

BLAST of ClCG10G011490 vs. NCBI nr
Match: XP_023525321.1 (pectinesterase inhibitor 10 [Cucurbita pepo subsp. pepo])

HSP 1 Score: 240.0 bits (611), Expect = 2.5e-59
Identity = 161/287 (56.10%), Postives = 195/287 (67.94%), Query Frame = 0

Query: 1   MELIN--NVFLLLALSSLFTSAIAVCVPRNTFNFGS---NSIPPVLSPQI--APQ-SLPV 60
           ME+IN  NVFLLLALSSLF  A  VCVPRN+  FGS   + I P  SP I  +PQ S+  
Sbjct: 46  MEIINGLNVFLLLALSSLFFPANTVCVPRNSAPFGSPVDSLIAPQASPSISSSPQPSVST 105

Query: 61  TVSPPQSTPSQAPSVTVSPPQSPPSQAPPATVSPPQSSTAPTNSAPAEEESGSEEHSGLP 120
           + SPP  +P+ +    ++  +S      P+ +  P  S + +  + A E S  +  S  P
Sbjct: 106 SSSPPAQSPTDSEDSALNSQESYSDAVSPSDMEFPFDSYSSSEKSEASEASSPQSPSSTP 165

Query: 121 VFHKLHSDDFVSSVTSSVTTDLAEICDVTSNPQLCRTSIASHINGPTVDPTTALKTEIEQ 180
                 SDD      S+V TDL EICDVTS+PQLC  SI+SHI+   V+PT+ALKTEIE+
Sbjct: 166 PTSASSSDD------STVPTDLEEICDVTSDPQLCTKSISSHIDESEVNPTSALKTEIEE 225

Query: 181 SIKEVSKAIATLNTLRKDSAASNAEIACYDTCLENFDMAIEDLKAGLESIDVHDNGRMES 240
           SIKEV KA+  L +LRK  +AS  EI+CYDTCLE FDMAI DL++GLESID  D G MES
Sbjct: 226 SIKEVRKAVTKLKSLRK--SASKVEISCYDTCLETFDMAIYDLQSGLESIDTKDTGTMES 285

Query: 241 VLTAVLTDLMTCDDTFAEMGVDSPLDSLSAKMSKYASNCLAISKLLL 280
           VL AV++DL TCDDTF EMGVDSPLD LS+KMSKYASNCLAISKLLL
Sbjct: 286 VLAAVMSDLTTCDDTFIEMGVDSPLDYLSSKMSKYASNCLAISKLLL 324

BLAST of ClCG10G011490 vs. NCBI nr
Match: KAG7036636.1 (hypothetical protein SDJN02_00256, partial [Cucurbita argyrosperma subsp. argyrosperma])

HSP 1 Score: 237.7 bits (605), Expect = 1.2e-58
Identity = 158/291 (54.30%), Postives = 196/291 (67.35%), Query Frame = 0

Query: 1   MELIN--NVFLLLALSSLFTSAIAVCVPRNTFNFGSNSIPPVLSPQIAPQ-------SLP 60
           ME+IN  NVFLLLALSSLF  A  VCVPRN+  FGS  +  +++PQ +P        S+ 
Sbjct: 46  MEIINGLNVFLLLALSSLFFPANTVCVPRNSAPFGS-PVDSLIAPQASPSQSSSPQPSVS 105

Query: 61  VTVSPPQSTPSQAPSVTVSPPQSPPSQAPPATVSPP---QSSTAPTNSAPAEEESGSEEH 120
            + SPP  +P+ +    ++  +S      P+ +  P    SS+  +  + A E S  +  
Sbjct: 106 TSSSPPAQSPTDSEDSALNSQESYSGALSPSDMEFPFDSYSSSEKSEKSEASEASSPQSP 165

Query: 121 SGLPVFHKLHSDDFVSSVTSSVTTDLAEICDVTSNPQLCRTSIASHINGPTVDPTTALKT 180
           S  P      SDD      S+V TDL EICDVTSNPQLC  SI+SHI+   V+PT+ALKT
Sbjct: 166 SSTPPTSASSSDD------STVPTDLEEICDVTSNPQLCTKSISSHIDESEVNPTSALKT 225

Query: 181 EIEQSIKEVSKAIATLNTLRKDSAASNAEIACYDTCLENFDMAIEDLKAGLESIDVHDNG 240
           EIE+SIKEV KA+  L +LRK  +AS  EI+CYDTCLE FDMAI DL++GLESI+  D G
Sbjct: 226 EIEESIKEVRKAVTKLKSLRK--SASKVEISCYDTCLETFDMAIYDLQSGLESIETKDTG 285

Query: 241 RMESVLTAVLTDLMTCDDTFAEMGVDSPLDSLSAKMSKYASNCLAISKLLL 280
            MESVL AV++DL TCDDTF EMGVDSPLD+LS+KMSKYASNCLAISKLLL
Sbjct: 286 TMESVLAAVMSDLTTCDDTFIEMGVDSPLDNLSSKMSKYASNCLAISKLLL 327

BLAST of ClCG10G011490 vs. ExPASy TrEMBL
Match: A0A0A0KWP8 (PMEI domain-containing protein OS=Cucumis sativus OX=3659 GN=Csa_4G003750 PE=4 SV=1)

HSP 1 Score: 306.6 bits (784), Expect = 1.1e-79
Identity = 184/287 (64.11%), Postives = 213/287 (74.22%), Query Frame = 0

Query: 1   MELIN----NVFLLLALSSLFTSAIAVCVPRNTFNFGSNSIPPVLSPQIAPQSLPVTVSP 60
           MELIN    NVFLLLA SS+F+ A+AVCVPRNT + GS +IPP+    ++PQ        
Sbjct: 1   MELINNSIHNVFLLLAFSSVFSFAVAVCVPRNTSDIGSTAIPPLFEQTVSPQ------VA 60

Query: 61  PQSTPSQAPSVTVSPPQSPPSQAPPATVSPPQSSTAPTNSAPAEEESGSEEHSGLPV--- 120
           PQ  P   P+VTVS     P  A P T     SS + +  A +EE +   E   L +   
Sbjct: 61  PQVAPQSPPAVTVS---EEPEAASPETSPSQPSSASESEEAESEEAASETEEDDLGLLGG 120

Query: 121 -FHKLHSDDFVSSVTSSVTTDLAEICDVTSNPQLCRTSIASHINGPTVDPTTALKTEIEQ 180
              +LHS D +SSVTSSVTT L +ICDVTSNPQLC+TSI+SHI G  VDP +ALKTEI++
Sbjct: 121 GLDQLHSADLISSVTSSVTTSLVDICDVTSNPQLCKTSISSHIEGTKVDPASALKTEIDE 180

Query: 181 SIKEVSKAIATLNTLRKDSAASNAEIACYDTCLENFDMAIEDLKAGLESIDVHDNGRMES 240
           SIKEV+KAIATLN+LRKDSAAS  EIACYDTCLENF+MAI+DLKAG+ESI+  D GRMES
Sbjct: 181 SIKEVAKAIATLNSLRKDSAASETEIACYDTCLENFEMAIDDLKAGVESINARDAGRMES 240

Query: 241 VLTAVLTDLMTCDDTFAEMGVDSPLDSLSAKMSKYASNCLAISKLLL 280
           VLTAV+TDL TCDDTFAEMGVDSPLDSLS KMSKYASNCLAISKLLL
Sbjct: 241 VLTAVMTDLTTCDDTFAEMGVDSPLDSLSTKMSKYASNCLAISKLLL 278

BLAST of ClCG10G011490 vs. ExPASy TrEMBL
Match: A0A5A7TT72 (Pectinesterase inhibitor 1 OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold655G001500 PE=4 SV=1)

HSP 1 Score: 297.7 bits (761), Expect = 4.9e-77
Identity = 179/281 (63.70%), Postives = 206/281 (73.31%), Query Frame = 0

Query: 4   INNVFLLLALSSLFTSAIAVCVPR--NTFNFGSNSIPPVLSPQIAPQSLPVTVSPPQSTP 63
           INNVFLLLA SS+F SAIA+CVP   N  +  S +IPP+L   ++PQ             
Sbjct: 8   INNVFLLLAFSSVFFSAIAICVPLNINITDVESTAIPPLLEQTVSPQ------------- 67

Query: 64  SQAPSVTVSPPQSPPSQAPPATVSPPQSSTAP---TNSAPAEEESGSEEHSGLPVFHKLH 123
                  V+P QSPP+     TVS P  +T+P   T+   +  E   EE+SGL     LH
Sbjct: 68  -------VAPDQSPPA---VTTVSQPPEATSPQTSTSDQSSSNEISDEENSGLLGLDYLH 127

Query: 124 SDDFVSSVTSSVTTDLAEICDVTSNPQLCRTSIASHINGPTVDPTTALKTEIEQSIKEVS 183
           SD  +S+  S+V T+L EICDVTSNPQLC+TSI+SHI G  VDP +ALKTEIE+SIKEV+
Sbjct: 128 SDGLISTAASTVKTNLVEICDVTSNPQLCKTSISSHIEGTKVDPASALKTEIEESIKEVT 187

Query: 184 KAIATLNTLRKDSAASNAEIACYDTCLENFDMAIEDLKAGLESIDVHDNGRMESVLTAVL 243
           KAIATLN+LRKDS+AS  EIACYDTCLENFDMAIEDLKAG+ESID  D GRMESVLTAVL
Sbjct: 188 KAIATLNSLRKDSSASETEIACYDTCLENFDMAIEDLKAGVESIDARDAGRMESVLTAVL 247

Query: 244 TDLMTCDDTFAEMGVDSPLDSLSAKMSKYASNCLAISKLLL 280
           TDL TCDDTFAEMGVDSPLD+LS KMSKYASNCLAISKLLL
Sbjct: 248 TDLTTCDDTFAEMGVDSPLDNLSTKMSKYASNCLAISKLLL 265

BLAST of ClCG10G011490 vs. ExPASy TrEMBL
Match: A0A1S3BYC7 (pectinesterase inhibitor 1 OS=Cucumis melo OX=3656 GN=LOC103494726 PE=4 SV=1)

HSP 1 Score: 297.7 bits (761), Expect = 4.9e-77
Identity = 179/281 (63.70%), Postives = 206/281 (73.31%), Query Frame = 0

Query: 4   INNVFLLLALSSLFTSAIAVCVPR--NTFNFGSNSIPPVLSPQIAPQSLPVTVSPPQSTP 63
           INNVFLLLA SS+F SAIA+CVP   N  +  S +IPP+L   ++PQ             
Sbjct: 8   INNVFLLLAFSSVFFSAIAICVPLNINITDVESTAIPPLLEQTVSPQ------------- 67

Query: 64  SQAPSVTVSPPQSPPSQAPPATVSPPQSSTAP---TNSAPAEEESGSEEHSGLPVFHKLH 123
                  V+P QSPP+     TVS P  +T+P   T+   +  E   EE+SGL     LH
Sbjct: 68  -------VAPDQSPPA---VTTVSQPPEATSPQTSTSDQSSSNEISDEENSGLLGLDYLH 127

Query: 124 SDDFVSSVTSSVTTDLAEICDVTSNPQLCRTSIASHINGPTVDPTTALKTEIEQSIKEVS 183
           SD  +S+  S+V T+L EICDVTSNPQLC+TSI+SHI G  VDP +ALKTEIE+SIKEV+
Sbjct: 128 SDGLISTAASTVKTNLVEICDVTSNPQLCKTSISSHIEGTKVDPASALKTEIEESIKEVT 187

Query: 184 KAIATLNTLRKDSAASNAEIACYDTCLENFDMAIEDLKAGLESIDVHDNGRMESVLTAVL 243
           KAIATLN+LRKDS+AS  EIACYDTCLENFDMAIEDLKAG+ESID  D GRMESVLTAVL
Sbjct: 188 KAIATLNSLRKDSSASETEIACYDTCLENFDMAIEDLKAGVESIDARDAGRMESVLTAVL 247

Query: 244 TDLMTCDDTFAEMGVDSPLDSLSAKMSKYASNCLAISKLLL 280
           TDL TCDDTFAEMGVDSPLD+LS KMSKYASNCLAISKLLL
Sbjct: 248 TDLTTCDDTFAEMGVDSPLDNLSTKMSKYASNCLAISKLLL 265

BLAST of ClCG10G011490 vs. ExPASy TrEMBL
Match: A0A6J1KAN1 (pectinesterase inhibitor 10 OS=Cucurbita maxima OX=3661 GN=LOC111493211 PE=4 SV=1)

HSP 1 Score: 236.9 bits (603), Expect = 1.0e-58
Identity = 159/291 (54.64%), Postives = 195/291 (67.01%), Query Frame = 0

Query: 1   MELIN--NVFLLLALSSLFTSAIAVCVPRNTFNFGSNSIPPVLSPQIAPQ-------SLP 60
           MELIN  NVFLLLALSSLF  A  VCVPRN+  FGS  +   ++PQ +P        S+ 
Sbjct: 46  MELINGLNVFLLLALSSLFFPANTVCVPRNSAPFGS-LVDSFVAPQASPSQSSSPQPSIS 105

Query: 61  VTVSPPQSTPSQAPSVTVSPPQSPPSQAPPATVSPP---QSSTAPTNSAPAEEESGSEEH 120
           ++ SPP  +P+ +    ++  +S      P+ +  P    SS+  + ++ A E S  +  
Sbjct: 106 ISPSPPAQSPTDSEDSALNSQESDSDALSPSDMEFPFDSYSSSEKSEASEASEASSPQSP 165

Query: 121 SGLPVFHKLHSDDFVSSVTSSVTTDLAEICDVTSNPQLCRTSIASHINGPTVDPTTALKT 180
           S  P      S D      S+V TDL EICDVTS+PQLC  SI+SHI+   V+PT+ALKT
Sbjct: 166 SSTPPTSASSSSD------STVPTDLEEICDVTSDPQLCTKSISSHIDESEVNPTSALKT 225

Query: 181 EIEQSIKEVSKAIATLNTLRKDSAASNAEIACYDTCLENFDMAIEDLKAGLESIDVHDNG 240
           EIE+SIKEV KA+  L  LRK  +AS  EIACYDTCLE FDMAI DL++GLESID  D G
Sbjct: 226 EIEESIKEVRKAVTKLKLLRK--SASKVEIACYDTCLETFDMAIFDLQSGLESIDTKDTG 285

Query: 241 RMESVLTAVLTDLMTCDDTFAEMGVDSPLDSLSAKMSKYASNCLAISKLLL 280
            MESVL AV++DL TCDDTF EMGVDSPLD+LS+KMSKYASNCLAISKLLL
Sbjct: 286 TMESVLAAVMSDLTTCDDTFIEMGVDSPLDNLSSKMSKYASNCLAISKLLL 327

BLAST of ClCG10G011490 vs. ExPASy TrEMBL
Match: A0A6J1GAW7 (pectinesterase inhibitor 10 OS=Cucurbita moschata OX=3662 GN=LOC111452501 PE=4 SV=1)

HSP 1 Score: 236.5 bits (602), Expect = 1.3e-58
Identity = 167/292 (57.19%), Postives = 194/292 (66.44%), Query Frame = 0

Query: 1   MELIN--NVFLLLALSSLFTSAIAVCVPRNTFNFGSNSIPPVLSPQIAPQSLPVTVSPPQ 60
           ME+IN  NVFLLLALSSLF  A  VCVPRN+  FGS    PV S  IAPQ+ P   S PQ
Sbjct: 46  MEIINGLNVFLLLALSSLFFPANTVCVPRNSAPFGS----PVDS-LIAPQASPSQSSSPQ 105

Query: 61  STPSQAPSVTVSPPQSPPSQAPPATVSPPQSSTAPT-----------NSAPAEEESGSEE 120
            + S +PS    P QSP +    A  S    S A +           +S+   E S  + 
Sbjct: 106 PSVSTSPS---PPAQSPTNSEDSALNSQESYSDALSPSDMEFPFDSYSSSEKSEASSPQS 165

Query: 121 HSGLPVFHKLHSDDFVSSVTSSVTTDLAEICDVTSNPQLCRTSIASHINGPTVDPTTALK 180
            S  P      SDD      S+V TDL EICDVTS+PQLC  SI+SHI+   V+PT+ALK
Sbjct: 166 PSSTPPTSASSSDD------STVPTDLEEICDVTSDPQLCTKSISSHIDESEVNPTSALK 225

Query: 181 TEIEQSIKEVSKAIATLNTLRKDSAASNAEIACYDTCLENFDMAIEDLKAGLESIDVHDN 240
           TEIE+SIKEV KA+  L +LRK  +AS  EI+CYDTCLE FDMAI DL++GLESID  D 
Sbjct: 226 TEIEESIKEVRKAVTKLKSLRK--SASKVEISCYDTCLETFDMAIYDLQSGLESIDTKDT 285

Query: 241 GRMESVLTAVLTDLMTCDDTFAEMGVDSPLDSLSAKMSKYASNCLAISKLLL 280
           G MESVL AV++DL TCDDTF EMGVDSPLD+LS+KMSKYASNCLAISKLLL
Sbjct: 286 GTMESVLAAVMSDLTTCDDTFIEMGVDSPLDNLSSKMSKYASNCLAISKLLL 321

BLAST of ClCG10G011490 vs. TAIR 10
Match: AT3G36659.1 (Plant invertase/pectin methylesterase inhibitor superfamily protein )

HSP 1 Score: 60.5 bits (145), Expect = 2.5e-09
Identity = 78/272 (28.68%), Postives = 124/272 (45.59%), Query Frame = 0

Query: 16  LFTSAIAVCVPRNTFNFGSNSIPPVLSPQIAPQSLPVTVSPPQSTPSQAPSVTVSPPQSP 75
           +FT +I V +   + +  S S  P  +P  AP+ +    SP  S         V+ P++P
Sbjct: 12  IFTVSILVTI---STHVESRSDSPFSTPAPAPEVMKPIASPANSPAEIYTDSPVASPEAP 71

Query: 76  PS----QAPPATV---SPPQSSTAPTNSAPAEEESGSEEHSGLPVFHKLHSDDFVSSVTS 135
                 + PP  +   SP  S  AP  S  A  ES S   SG+    K+ S         
Sbjct: 72  ADSFSPKNPPVEIDIYSPAASPEAPVASPEAPAESSSAAPSGI----KVSSSPKSLLNPP 131

Query: 136 SVTTDLAEICDVTSNPQLCRTSIASHINGPTVDPTTA--LKTEIEQSIKEVSKAIATLNT 195
            ++ ++  IC  T NP LC +S+ S +  P + P T+  L   I+ SI     A+A +  
Sbjct: 132 LLSPEIKTICGKTDNPPLCESSV-SPLLTPQLKPNTSSVLILAIQASITATKAAMAIVEK 191

Query: 196 LRKDSAASNAEIACYDTCLENFDMAIEDLKAGLESIDVHDNGRMESVLTAVLTDLMTCDD 255
           +                C E +D A+ +L+  + ++   D   + + L+A +TD  TC+D
Sbjct: 192 VDASD------------CQELYDDAVVNLEDAVNAVKSSDIATVNTNLSAAMTDYSTCND 251

Query: 256 TFAEMGVDSPLDSLSAKMSKYASNCLAISKLL 279
            F E G  +PL  ++ K++K  SNCLAIS L+
Sbjct: 252 GFEESGEPNPLAYVADKLTKMVSNCLAISTLI 263

BLAST of ClCG10G011490 vs. TAIR 10
Match: AT1G23350.1 (Plant invertase/pectin methylesterase inhibitor superfamily protein )

HSP 1 Score: 56.6 bits (135), Expect = 3.7e-08
Identity = 41/152 (26.97%), Postives = 73/152 (48.03%), Query Frame = 0

Query: 127 TSSVTTDLAEICDVTSNPQLCRTSIASHINGPTVDPTTALKTEIEQSIKEVSKAIATLNT 186
           + +++  LA+ C V+  P LCR++I    N         +    + +IKE+ K       
Sbjct: 18  SQALSISLADPCTVSDFPALCRSTIKGQTN---------VNAATDVAIKELMKRTRQAKK 77

Query: 187 LRKDSAASNAEIACYDTCLENFDMAIEDLKAGLESIDVHDNGRMESVLTAVLTDLMTCDD 246
           + +     +  +A   TCL NF+ A ++L+  L +I  +D   +   L+A LTD  TC D
Sbjct: 78  IAEKELKRDGGVA---TCLSNFNSAFDNLEKALTNIKENDGFSLNINLSAALTDYDTCSD 137

Query: 247 TFAEMGVDSPLDSLSAKMSKYASNCLAISKLL 279
           +  E    + +   +  + + A NCLA+S L+
Sbjct: 138 SMKETKEVNVIYKSAGVLYRMADNCLALSTLV 157

BLAST of ClCG10G011490 vs. TAIR 10
Match: AT2G47340.1 (Plant invertase/pectin methylesterase inhibitor superfamily protein )

HSP 1 Score: 51.6 bits (122), Expect = 1.2e-06
Identity = 64/237 (27.00%), Postives = 104/237 (43.88%), Query Frame = 0

Query: 51  PVTVSPPQST----PSQAPSVTVSPPQSPPSQAPPATVSPPQSSTAPTNSAPAEEESGSE 110
           P  + PP S+    P+ APS T     +  + AP A VS P  S           +S + 
Sbjct: 45  PSLIEPPVSSISVVPASAPSPTSEDINTGSNLAPTAAVSAPGPS---QEDIDINFDSVTN 104

Query: 111 EHSGLPVFHKLHSDDFVSSVTSSVTTDLAEICDVTSN------PQLCRTSIASHINGPTV 170
                P F  ++  DF S    +   DL +  D  +       P L + +     +G  +
Sbjct: 105 IADLAPKFEHINKFDFSSMKIDTTAKDLCKNTDYNNECIAAILPDLQKQAEGEGGDG-GI 164

Query: 171 DPTTALKTEIEQSIKEVSKAIATLNTLRKDSAASNAEIACYDTCLENFDMAIEDLKAGLE 230
            P   ++ E E  +K+ +  +     + +D             C+EN+D  I  L+    
Sbjct: 165 APKDVMRMEAESLLKKANATLDYAKRVVEDRKTPMPVKEAMSVCVENYDSLISGLEDAKM 224

Query: 231 SIDVHDNGRMESVLTAVLTDLMTCDDTFAEM-GVDSPLDSLSAKMSKYASNCLAISK 277
           +++  D GR++SVL+A ++D+ TC D F ++  VDSP  SL   M K  SN LA+S+
Sbjct: 225 AMEEGDYGRLDSVLSAAISDVSTCSDNFVDIPDVDSPTASLDDLMKKLCSNVLAMSQ 277

BLAST of ClCG10G011490 vs. TAIR 10
Match: AT1G02550.1 (Plant invertase/pectin methylesterase inhibitor superfamily protein )

HSP 1 Score: 47.4 bits (111), Expect = 2.2e-05
Identity = 63/231 (27.27%), Postives = 100/231 (43.29%), Query Frame = 0

Query: 59  STPSQAPSVTVSPPQSPPSQAPPATVSPPQSSTAPTNSAPAEEESG-------SEEHSGL 118
           S+PS +  +  S     PS AP   V+P        N   A  +               L
Sbjct: 19  SSPSSSTRIISS---IVPSAAPSPAVAPTTDGDVDENDFSAFTQWNILNLTDLKSTFKNL 78

Query: 119 PVFHKLH-SDDFVSSVTSSV--TTDLAEICDVTSNPQLCRTSIASHINGPTVDPTTALKT 178
           P F KL+ S   VS    SV   TD A  C V+  P L               P   L+ 
Sbjct: 79  PDFSKLNISSLHVSPAVGSVCSNTDYAAECIVSILPLL------RDFRKFEPKPIDVLRM 138

Query: 179 EIEQSIKEVSKAIATLNTLRKDSAASNAEIACYDTCLENFDMAIEDLKAGLESIDVHDNG 238
           E+    ++ +  +     L  D +         D C++N++  ++DLK    ++D  D  
Sbjct: 139 EMSALYEKANATLDLAKRLIVDKSTPRDVADVLDLCVDNYESLLDDLKDASVAVDDGDFE 198

Query: 239 RMESVLTAVLTDLMTCDDTFAEMG-VDSPLDSLSAKMSKYASNCLAISKLL 279
           R+ESV++A + D++TC D FAE   ++SP+ ++   + K  SN LAIS+++
Sbjct: 199 RLESVVSAAIADVVTCSDAFAESSELESPMANVDDFLKKLCSNVLAISQMI 240

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
XP_038903716.14.8e-10378.6721 kDa protein-like [Benincasa hispida][more]
XP_011654315.29.8e-8064.24uncharacterized protein LOC105435336 [Cucumis sativus] >KAE8649055.1 hypothetica... [more]
XP_008454280.11.0e-7663.70PREDICTED: pectinesterase inhibitor 1 [Cucumis melo] >KAA0044419.1 pectinesteras... [more]
XP_023525321.12.5e-5956.10pectinesterase inhibitor 10 [Cucurbita pepo subsp. pepo][more]
KAG7036636.11.2e-5854.30hypothetical protein SDJN02_00256, partial [Cucurbita argyrosperma subsp. argyro... [more]
Match NameE-valueIdentityDescription
Match NameE-valueIdentityDescription
A0A0A0KWP81.1e-7964.11PMEI domain-containing protein OS=Cucumis sativus OX=3659 GN=Csa_4G003750 PE=4 S... [more]
A0A5A7TT724.9e-7763.70Pectinesterase inhibitor 1 OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaff... [more]
A0A1S3BYC74.9e-7763.70pectinesterase inhibitor 1 OS=Cucumis melo OX=3656 GN=LOC103494726 PE=4 SV=1[more]
A0A6J1KAN11.0e-5854.64pectinesterase inhibitor 10 OS=Cucurbita maxima OX=3661 GN=LOC111493211 PE=4 SV=... [more]
A0A6J1GAW71.3e-5857.19pectinesterase inhibitor 10 OS=Cucurbita moschata OX=3662 GN=LOC111452501 PE=4 S... [more]
Match NameE-valueIdentityDescription
AT3G36659.12.5e-0928.68Plant invertase/pectin methylesterase inhibitor superfamily protein [more]
AT1G23350.13.7e-0826.97Plant invertase/pectin methylesterase inhibitor superfamily protein [more]
AT2G47340.11.2e-0627.00Plant invertase/pectin methylesterase inhibitor superfamily protein [more]
AT1G02550.12.2e-0527.27Plant invertase/pectin methylesterase inhibitor superfamily protein [more]
InterPro
Analysis Name: InterPro Annotations of Watermelon (Charleston Gray) v2.5
Date Performed: 2022-01-31
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR006501Pectinesterase inhibitor domainSMARTSM00856PMEI_2coord: 129..274
e-value: 5.8E-22
score: 89.0
IPR006501Pectinesterase inhibitor domainTIGRFAMTIGR01614TIGR01614coord: 129..278
e-value: 1.2E-22
score: 78.1
IPR006501Pectinesterase inhibitor domainPFAMPF04043PMEIcoord: 134..274
e-value: 3.1E-19
score: 69.8
IPR035513Invertase/pectin methylesterase inhibitor domain superfamilyGENE3D1.20.140.40Invertase/pectin methylesterase inhibitor family proteincoord: 134..278
e-value: 7.8E-28
score: 99.3
IPR035513Invertase/pectin methylesterase inhibitor domain superfamilySUPERFAMILY101148Plant invertase/pectin methylesterase inhibitorcoord: 134..278
NoneNo IPR availableMOBIDB_LITEmobidb-litedisorder_predictioncoord: 49..67
NoneNo IPR availableMOBIDB_LITEmobidb-litedisorder_predictioncoord: 49..111
NoneNo IPR availableMOBIDB_LITEmobidb-litedisorder_predictioncoord: 68..87
NoneNo IPR availablePANTHERPTHR31707PECTINESTERASEcoord: 24..273
NoneNo IPR availablePANTHERPTHR31707:SF119INVERTASE-RELATEDcoord: 24..273
NoneNo IPR availableCDDcd15800PMEI-like_2coord: 133..278
e-value: 1.16298E-47
score: 154.055

Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
ClCG10G011490.1ClCG10G011490.1mRNA


GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
molecular_function GO:0030599 pectinesterase activity
molecular_function GO:0046910 pectinesterase inhibitor activity
molecular_function GO:0004857 enzyme inhibitor activity