ClCG10G010350 (gene) Watermelon (Charleston Gray) v2.5

Overview


Name	ClCG10G010350
Type	gene
Organism	Citrullus lanatus subsp. vulgaris cv. Charleston Gray (Watermelon (Charleston Gray) v2.5)
Description	trypsin inhibitor 1-like
Location	CG_Chr10: 23069890 .. 23070144 (+)
RNA-Seq Expression	ClCG10G010350
Synteny	ClCG10G010350

Sequences

The following sequences are available for this feature:

Gene sequence (with intron)

Legend: exonCDSpolypeptide

Hold the cursor over a type above to highlight its positions in the sequence below.

ATGGAGTGGAAGAGAGTTGGGTTGGTGGCTGTGGTAGGGATGCTGATAATGGCTGCTTTGGTGGAGGAGTGTGTAGCCATTGAAGCACAGTCTGTGGGAGGTTTGAATAAAAATGAATTCCCAAGAAAGATGATGAATGAAGATTATGATAATGTTAATAAAGGAAGAATGTGCCCTAGAATCCTTCAGGAGTGCACTACTGATGATGATTGCATGAATGACTGCATTTGCCTCTCTAATGGCTTTTGTGGTTGA

mRNA sequence

ATGGAGTGGAAGAGAGTTGGGTTGGTGGCTGTGGTAGGGATGCTGATAATGGCTGCTTTGGTGGAGGAGTGTGTAGCCATTGAAGCACAGTCTGTGGGAGGTTTGAATAAAAATGAATTCCCAAGAAAGATGATGAATGAAGATTATGATAATGTTAATAAAGGAAGAATGTGCCCTAGAATCCTTCAGGAGTGCACTACTGATGATGATTGCATGAATGACTGCATTTGCCTCTCTAATGGCTTTTGTGGTTGA

Coding sequence (CDS)

ATGGAGTGGAAGAGAGTTGGGTTGGTGGCTGTGGTAGGGATGCTGATAATGGCTGCTTTGGTGGAGGAGTGTGTAGCCATTGAAGCACAGTCTGTGGGAGGTTTGAATAAAAATGAATTCCCAAGAAAGATGATGAATGAAGATTATGATAATGTTAATAAAGGAAGAATGTGCCCTAGAATCCTTCAGGAGTGCACTACTGATGATGATTGCATGAATGACTGCATTTGCCTCTCTAATGGCTTTTGTGGTTGA

Protein sequence

MEWKRVGLVAVVGMLIMAALVEECVAIEAQSVGGLNKNEFPRKMMNEDYDNVNKGRMCPRILQECTTDDDCMNDCICLSNGFCG

Homology

[Close]

BLAST of ClCG10G010350 vs. NCBI nr
Match: XP_031739937.1 (uncharacterized protein LOC105435333 [Cucumis sativus])

HSP 1 Score: 131.7 bits (330), Expect = 2.9e-27
Identity = 64/85 (75.29%), Postives = 74/85 (87.06%), Query Frame = 0


Query: 1  MEWKRVGLVAVVGMLIMAALVEEC-VAIEAQSVGGLNKNEFPRKMMNEDYDNVNKGRMCP 60
          MEWK++G++AVVGML+MAALVE+C  AIE++SV GLNKNE PRKMMNE+       RMCP
Sbjct: 1  MEWKKIGMLAVVGMLVMAALVEDCHAAIESESVKGLNKNELPRKMMNEE------TRMCP 60

Query: 61 RILQECTTDDDCMNDCICLSNGFCG 85
          RIL+ECTTDDDCMNDCICLSNGFCG
Sbjct: 61 RILEECTTDDDCMNDCICLSNGFCG 79

[Close]

BLAST of ClCG10G010350 vs. NCBI nr
Match: KGN52760.1 (hypothetical protein Csa_014741 [Cucumis sativus])

HSP 1 Score: 131.7 bits (330), Expect = 2.9e-27
Identity = 64/85 (75.29%), Postives = 74/85 (87.06%), Query Frame = 0


Query: 1  MEWKRVGLVAVVGMLIMAALVEEC-VAIEAQSVGGLNKNEFPRKMMNEDYDNVNKGRMCP 60
          MEWK++G++AVVGML+MAALVE+C  AIE++SV GLNKNE PRKMMNE+       RMCP
Sbjct: 1  MEWKKIGMLAVVGMLVMAALVEDCHAAIESESVKGLNKNELPRKMMNEE------TRMCP 60

Query: 61 RILQECTTDDDCMNDCICLSNGFCG 85
          RIL+ECTTDDDCMNDCICLSNGFCG
Sbjct: 61 RILEECTTDDDCMNDCICLSNGFCG 79

[Close]

BLAST of ClCG10G010350 vs. NCBI nr
Match: KAG6576915.1 (hypothetical protein SDJN03_24489, partial [Cucurbita argyrosperma subsp. sororia])

HSP 1 Score: 74.7 bits (182), Expect = 4.2e-10
Identity = 41/87 (47.13%), Postives = 52/87 (59.77%), Query Frame = 0


Query: 1   MEWKRVGLVAVVGMLIMAALVEECVAIEAQSVGGLNK--NEFPRKMMNEDYDNVNKGRM- 60
           MEWKR  L+A+VG+L+MAA  E       + +  ++   N+FPRKMMN        G + 
Sbjct: 83  MEWKRGALIAMVGLLLMAAFAESAAVHGGEVIQLVSDGVNDFPRKMMN--------GALG 142

Query: 61  CPRILQECTTDDDCMNDCICLSNGFCG 85
           CPRIL EC  D DC+  CIC  NGFCG
Sbjct: 143 CPRILMECEVDSDCLPGCICRPNGFCG 161

[Close]

BLAST of ClCG10G010350 vs. NCBI nr
Match: KAG7014942.1 (hypothetical protein SDJN02_22573, partial [Cucurbita argyrosperma subsp. argyrosperma])

HSP 1 Score: 74.7 bits (182), Expect = 4.2e-10
Identity = 41/87 (47.13%), Postives = 52/87 (59.77%), Query Frame = 0


Query: 1   MEWKRVGLVAVVGMLIMAALVEECVAIEAQSVGGLNK--NEFPRKMMNEDYDNVNKGRM- 60
           MEWKR  L+A+VG+L+MAA  E       + +  ++   N+FPRKMMN        G + 
Sbjct: 83  MEWKRGALIAMVGLLLMAAFAESAAVHGGEVIQLVSDGVNDFPRKMMN--------GALG 142

Query: 61  CPRILQECTTDDDCMNDCICLSNGFCG 85
           CPRIL EC  D DC+  CIC  NGFCG
Sbjct: 143 CPRILMECEVDSDCLPGCICRPNGFCG 161

[Close]

BLAST of ClCG10G010350 vs. NCBI nr
Match: XP_022984819.1 (trypsin inhibitor 1-like [Cucurbita maxima])

HSP 1 Score: 73.6 bits (179), Expect = 9.4e-10
Identity = 40/86 (46.51%), Postives = 51/86 (59.30%), Query Frame = 0


Query: 1  MEWKRVGLVAVVGMLIMAALVEECVAIEAQSVGGLNK--NEFPRKMMNEDYDNVNKGRMC 60
          MEWKR  L+A+VG+L+MAA  E       + +  ++   N+FPRKMM       N  + C
Sbjct: 1  MEWKRGALIAMVGLLLMAAFAESAAVHGGEVIQLVSDGVNDFPRKMM-------NGVQGC 60

Query: 61 PRILQECTTDDDCMNDCICLSNGFCG 85
          PRIL EC  D DC+  CIC  NGFCG
Sbjct: 61 PRILMECELDSDCLPGCICRPNGFCG 79

[Close]

BLAST of ClCG10G010350 vs. ExPASy Swiss-Prot
Match: P34950 (Trypsin inhibitor 5 OS=Luffa aegyptiaca OX=3670 PE=1 SV=1)

HSP 1 Score: 62.4 bits (150), Expect = 2.8e-09
Identity = 31/68 (45.59%), Postives = 41/68 (60.29%), Query Frame = 0


Query: 17 MAALVEECVAIEAQSVGGLNKNEFPRKMMNEDYDNVNKGRMCPRILQECTTDDDCMNDCI 76
          MA++ E    +E   +     N+ PRK+M+  +     G +CPRIL  C TDDDCM DC 
Sbjct: 1  MASVAESSGVVEVIELISDGGNDLPRKIMSGRH-----GGICPRILMPCKTDDDCMLDCR 60

Query: 77 CLSNGFCG 85
          CLSNG+CG
Sbjct: 61 CLSNGYCG 63

[Close]

BLAST of ClCG10G010350 vs. ExPASy Swiss-Prot
Match: Q9S8I2 (Trypsin inhibitor 3 OS=Luffa aegyptiaca OX=3670 PE=1 SV=1)

HSP 1 Score: 54.3 bits (129), Expect = 7.7e-07
Identity = 20/29 (68.97%), Postives = 25/29 (86.21%), Query Frame = 0


Query: 56 RMCPRILQECTTDDDCMNDCICLSNGFCG 85
          R+CPRIL EC++D DC+ +CICL NGFCG
Sbjct: 1  RICPRILMECSSDSDCLAECICLENGFCG 29

[Close]

BLAST of ClCG10G010350 vs. ExPASy Swiss-Prot
Match: P25849 (Trypsin inhibitor 1 OS=Luffa aegyptiaca OX=3670 PE=1 SV=1)

HSP 1 Score: 52.0 bits (123), Expect = 3.8e-06
Identity = 19/29 (65.52%), Postives = 24/29 (82.76%), Query Frame = 0


Query: 56 RMCPRILQECTTDDDCMNDCICLSNGFCG 85
          R+CPRIL EC++D DC+ +CICL  GFCG
Sbjct: 1  RICPRILMECSSDSDCLAECICLEQGFCG 29

[Close]

BLAST of ClCG10G010350 vs. ExPASy Swiss-Prot
Match: P35628 (Trypsin inhibitor 4 OS=Luffa aegyptiaca OX=3670 PE=1 SV=1)

HSP 1 Score: 50.4 bits (119), Expect = 1.1e-05
Identity = 18/28 (64.29%), Postives = 23/28 (82.14%), Query Frame = 0


Query: 57 MCPRILQECTTDDDCMNDCICLSNGFCG 85
          +CPRIL  C++D DC+ +CICL NGFCG
Sbjct: 1  ICPRILMPCSSDSDCLAECICLENGFCG 28

[Close]

BLAST of ClCG10G010350 vs. ExPASy Swiss-Prot
Match: P32041 (Trypsin inhibitor 3 OS=Cucumis melo var. conomon OX=3657 PE=1 SV=1)

HSP 1 Score: 50.1 bits (118), Expect = 1.5e-05
Identity = 18/29 (62.07%), Postives = 22/29 (75.86%), Query Frame = 0


Query: 56 RMCPRILQECTTDDDCMNDCICLSNGFCG 85
          RMCP+IL +C  D DC+ DC+CL  GFCG
Sbjct: 2  RMCPKILMKCKQDSDCLLDCVCLKEGFCG 30

[Close]

BLAST of ClCG10G010350 vs. ExPASy TrEMBL
Match: A0A0A0KT01 (Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_4G000805 PE=3 SV=1)

HSP 1 Score: 131.7 bits (330), Expect = 1.4e-27
Identity = 64/85 (75.29%), Postives = 74/85 (87.06%), Query Frame = 0


Query: 1  MEWKRVGLVAVVGMLIMAALVEEC-VAIEAQSVGGLNKNEFPRKMMNEDYDNVNKGRMCP 60
          MEWK++G++AVVGML+MAALVE+C  AIE++SV GLNKNE PRKMMNE+       RMCP
Sbjct: 1  MEWKKIGMLAVVGMLVMAALVEDCHAAIESESVKGLNKNELPRKMMNEE------TRMCP 60

Query: 61 RILQECTTDDDCMNDCICLSNGFCG 85
          RIL+ECTTDDDCMNDCICLSNGFCG
Sbjct: 61 RILEECTTDDDCMNDCICLSNGFCG 79

[Close]

BLAST of ClCG10G010350 vs. ExPASy TrEMBL
Match: A0A6J1JBL8 (trypsin inhibitor 1-like OS=Cucurbita maxima OX=3661 GN=LOC111482991 PE=3 SV=1)

HSP 1 Score: 73.6 bits (179), Expect = 4.5e-10
Identity = 40/86 (46.51%), Postives = 51/86 (59.30%), Query Frame = 0


Query: 1  MEWKRVGLVAVVGMLIMAALVEECVAIEAQSVGGLNK--NEFPRKMMNEDYDNVNKGRMC 60
          MEWKR  L+A+VG+L+MAA  E       + +  ++   N+FPRKMM       N  + C
Sbjct: 1  MEWKRGALIAMVGLLLMAAFAESAAVHGGEVIQLVSDGVNDFPRKMM-------NGVQGC 60

Query: 61 PRILQECTTDDDCMNDCICLSNGFCG 85
          PRIL EC  D DC+  CIC  NGFCG
Sbjct: 61 PRILMECELDSDCLPGCICRPNGFCG 79

[Close]

BLAST of ClCG10G010350 vs. ExPASy TrEMBL
Match: A0A5D3E148 (Trypsin inhibitor 1-like OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold655G00640 PE=3 SV=1)

HSP 1 Score: 63.2 bits (152), Expect = 6.1e-07
Identity = 34/92 (36.96%), Postives = 49/92 (53.26%), Query Frame = 0


Query: 1  MEWKRVGLVAVVGMLIMAALVEECVAIEAQSVGGLN--------KNEFPRKMMNEDYDNV 60
          M+ K++ +VA+VGM+IM A +    +         N         N  PRK+M +     
Sbjct: 1  MDSKKIVVVAIVGMIIMLAAMSSSASFVVDDKDSTNLVSDGRDMNNGCPRKIMIKGGVFK 60

Query: 61 NKGRMCPRILQECTTDDDCMNDCICLSNGFCG 85
           + RMCP+IL +C  D DC+ DC+CL  GFCG
Sbjct: 61 EEQRMCPKILMKCKQDSDCLLDCVCLKEGFCG 92

[Close]

BLAST of ClCG10G010350 vs. ExPASy TrEMBL
Match: A0A5D3E0W0 (Trypsin inhibitor 1 OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold655G00600 PE=3 SV=1)

HSP 1 Score: 60.8 bits (146), Expect = 3.0e-06
Identity = 31/84 (36.90%), Postives = 47/84 (55.95%), Query Frame = 0


Query: 1  MEWKRVGLVAVVGMLIMAALVEECVAIEAQSVGGLNKNEFPRKMMNEDYDNVNKGRMCPR 60
          MEWK++ LVA++GML+MA   E           G   +E   +++++  +  +    CPR
Sbjct: 1  MEWKKIALVAMMGMLLMATFTESV---------GFGVDEEIIQLVSDGVNEYSGHPGCPR 60

Query: 61 ILQECTTDDDCMNDCICLSNGFCG 85
          IL +C TD DC+  C C  NG+CG
Sbjct: 61 ILMKCKTDRDCLAGCTCKRNGYCG 75

[Close]

BLAST of ClCG10G010350 vs. ExPASy TrEMBL
Match: A0A0A0KY34 (Trypsin inhibitor 1 OS=Cucumis sativus OX=3659 GN=Csa_4G000810 PE=3 SV=1)

HSP 1 Score: 58.2 bits (139), Expect = 2.0e-05
Identity = 36/89 (40.45%), Postives = 49/89 (55.06%), Query Frame = 0


Query: 1  MEWKRVGLVAVVGMLIMAALVEECVAIEAQSVG-GLNKN--EFPRKMMNEDYDNVNKGRM 60
          MEWK++ LVA++GML+MA   E        SVG G+++   +     +NE   N+ K   
Sbjct: 1  MEWKKIALVAMMGMLLMATFTE--------SVGLGVDEEIIQLVSDGVNEYSQNIGKDTA 60

Query: 61 --CPRILQECTTDDDCMNDCICLSNGFCG 85
            CPRIL +C TD DC   C C   G+CG
Sbjct: 61 PGCPRILMKCKTDSDCYPGCTCKPMGYCG 81

The following BLAST results are available for this feature:

BLAST of ClCG10G010350 vs. NCBI nr


Match Name	E-value	Identity	Description
XP_031739937.1	2.9e-27	75.29	uncharacterized protein LOC105435333 [Cucumis sativus]	[more]
KGN52760.1	2.9e-27	75.29	hypothetical protein Csa_014741 [Cucumis sativus]	[more]
KAG6576915.1	4.2e-10	47.13	hypothetical protein SDJN03_24489, partial [Cucurbita argyrosperma subsp. sorori...	[more]
KAG7014942.1	4.2e-10	47.13	hypothetical protein SDJN02_22573, partial [Cucurbita argyrosperma subsp. argyro...	[more]
XP_022984819.1	9.4e-10	46.51	trypsin inhibitor 1-like [Cucurbita maxima]	[more]

BLAST of ClCG10G010350 vs. ExPASy Swiss-Prot


Match Name	E-value	Identity	Description
P34950	2.8e-09	45.59	Trypsin inhibitor 5 OS=Luffa aegyptiaca OX=3670 PE=1 SV=1	[more]
Q9S8I2	7.7e-07	68.97	Trypsin inhibitor 3 OS=Luffa aegyptiaca OX=3670 PE=1 SV=1	[more]
P25849	3.8e-06	65.52	Trypsin inhibitor 1 OS=Luffa aegyptiaca OX=3670 PE=1 SV=1	[more]
P35628	1.1e-05	64.29	Trypsin inhibitor 4 OS=Luffa aegyptiaca OX=3670 PE=1 SV=1	[more]
P32041	1.5e-05	62.07	Trypsin inhibitor 3 OS=Cucumis melo var. conomon OX=3657 PE=1 SV=1	[more]

BLAST of ClCG10G010350 vs. ExPASy TrEMBL


Match Name	E-value	Identity	Description
A0A0A0KT01	1.4e-27	75.29	Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_4G000805 PE=3 SV=1	[more]
A0A6J1JBL8	4.5e-10	46.51	trypsin inhibitor 1-like OS=Cucurbita maxima OX=3661 GN=LOC111482991 PE=3 SV=1	[more]
A0A5D3E148	6.1e-07	36.96	Trypsin inhibitor 1-like OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffol...	[more]
A0A5D3E0W0	3.0e-06	36.90	Trypsin inhibitor 1 OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold655G...	[more]
A0A0A0KY34	2.0e-05	40.45	Trypsin inhibitor 1 OS=Cucumis sativus OX=3659 GN=Csa_4G000810 PE=3 SV=1	[more]

BLAST of ClCG10G010350 vs. ExPASy TrEMBL


Match Name	E-value	Identity	Description

InterPro

Analysis Name: InterPro Annotations of Watermelon (Charleston Gray) v2.5
Date Performed: 2022-01-31


IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
None	No IPR available	SMART	SM00286	PTI_1	coord: 56..84 e-value: 5.3E-12 score: 55.9
None	No IPR available	GENE3D	4.10.75.20		coord: 35..84 e-value: 2.6E-16 score: 61.2
IPR000737	Proteinase inhibitor I7, squash	PFAM	PF00299	Squash	coord: 56..84 e-value: 7.9E-15 score: 54.6
IPR000737	Proteinase inhibitor I7, squash	PROSITE	PS00286	SQUASH_INHIBITOR	coord: 58..77
IPR000737	Proteinase inhibitor I7, squash	CDD	cd00150	PlantTI	coord: 58..84 e-value: 1.75862E-10 score: 48.9131
IPR011052	Proteinase/amylase inhibitor domain superfamily	SUPERFAMILY	57027	Plant inhibitors of proteinases and amylases	coord: 54..84

Relationships

The following mRNA feature(s) are a part of this gene:


Feature Name	Unique Name	Type
ClCG10G010350.1	ClCG10G010350.1	mRNA

GO Annotation

GO Assignments

This gene is annotated with the following GO terms.

Category	Term Accession	Term Name
biological_process	GO:0010951	negative regulation of endopeptidase activity
cellular_component	GO:0005576	extracellular region
molecular_function	GO:0004867	serine-type endopeptidase inhibitor activity
molecular_function	GO:0004866	endopeptidase inhibitor activity