ClCG10G002750 (gene) Watermelon (Charleston Gray) v2.5

Overview


Name	ClCG10G002750
Type	gene
Organism	Citrullus lanatus subsp. vulgaris cv. Charleston Gray (Watermelon (Charleston Gray) v2.5)
Description	HMA domain-containing protein
Location	CG_Chr10: 3178999 .. 3181427 (-)
RNA-Seq Expression	ClCG10G002750
Synteny	ClCG10G002750

Sequences

The following sequences are available for this feature:

Gene sequence (with intron)

Legend: polypeptideexonCDS

Hold the cursor over a type above to highlight its positions in the sequence below.

ATGGAGCAATCTTTGTTATTTTCTGAGAATCTAACACCCCCTTCTGTAAGATTTTTCAATTATATTATTGCTTTCTTTCACTCTCTCTTTTTCTTAGTTTGACGTTATTCTGAAAATTTGAATATTTGACATCTAGATCGATCTAAAATACATGTTTTCTTTGGGGATATAAGAGATGTATAATAATGTTTTTGTTTGTTTGATATATAGTTTCAGGTGATTGTTATAAATGCAAATATGAGTTGCAATGGATGTCGCCAAAGAGTTTCTCAAATTGTCTCTAAAATGTCAGGTTGGTATTTTTCATTATTTATTGATTTGCTTCTTCACTTCTTCCTTCTTCTTTTAACATAATTTAAACCTAATTTGGAATTTAAAAAAATATGATCTTTTTCTTTCGTTTTACTTTAAAAGTAGTCGATGTAATTTTCATAGTTTGTTATATTATATAATATTTAAATTAATTAGGATTGATGTGATTTCAAATATTGAGTGAAGTGGAAGATTCAAACTTCAAATCTTTTGATCGAGTGCATATATTTTAATTAGTTACACTATGCTTAAGTTAGTTGTATAGTTTCTTGTTATCTCTTGATTAATATCACAGTATTCCCTAAAGTTTAAGAATGCAATTTAAACTCGAATTTAAAAAAATATGATCTTTTTCTTTCGTTTTACTTTAAAAGTAGTCAATGTAATTTTCATAGTTTGTTATATTATATAATATTTAAATTAATTAGGATTGATGTGATTTCAAATATTGAGTGAAGTGGAAGATTCAAACTTCAAATCTTTTGATCGAGTGCATATATTTTAATTAGTTACACTCTGCTTAAGTTAGTTGTATAGTTTCTTGTTATCTCTTGATTAATATCACAGTATTCCCTAAAGTTTAAGAATGCAATTTAAACTTTTGAATTTTTAGAAACTTCATTTTTTAAGTTTAAACAATATACATATGATGAGAGGATTCAAATATTTATAGGGTCTGGTTGGTAGAGAATTCAAATTCTATTTTAGGTCTTCAAATTCATTGAGTTAAACAAATATGTTTTTTGTAGACAACTCGAATTTTGTTTCTAAATACTTCTGTGGTCCAAGTAGTGCAAATTCTAAAATCAATATATTTTTTTTATTACAGTACACAAAAGTCAAGGGATTTAAACTTCCAACTTTAAAATAGGTAGTAATACATGTCAATTATCATTAAACTATGCTTAACTTTGACAGGTAAATGACAATATTCCTATTGGTATAAGACATCTTGAATGAAACTTGAAGTAAATATATGCATGTCTTAAACACAAAAAAGATATAGCAGTCAATTGTGGAAATGATAGATTATCCTTATAAGTAGTGATATTAGATTAGTCATGTCTTGGCCTCCTCCATGGAGTTCATAACAAAAAATAACAAAAAAAAACTGTGGGACTCTTGAAAATATATAACTGTAAGGCCCTAAGGTTCAAGATTCGAACTAGGATTGATAATCTAAATTCAACACTTGATAGTCTCGACATTTTCCTACATCTACTGCCACCTGATAATGCCATCCTCTCCTATCTTGAATTGCTTTTAAGATGAAGACTATTCCCACAAATTGACATAGATCCTTTTGACATACTTTGTCCTCACTCACATGCTACTTAGAAAAGTTTTTAAGAGGTTGTCTAGCAAAAATTGCTCCAAATAAAACACGCTTAACTGTGAAGTTCCTATGATTGGACTCCGAAGAGGAGTGTGCACCTTATTAATATAGGTGCTAAATATCAATTCTTTTAAGCAATTTTTGAACCATATTTTCATATTCTTGAATATGATCTTGGTTCGTTTTTATCATCCTCCTAAACTTAGGGCATTACAAACCTTCTGGGCATTACAAACCTTCTATAAAAAATGTAGGATTAATGTAGGATTATAAAAAAAAAAAAAAAAAAAAAAAAGAAAAGAAAAAATCTTCCTTTGAAGAGGGAGGATTATAGAAAATAGGAATAACCTAAATAGAAAGAGATACATCTATGAAAAAAGCAAGCAACATCTTACATCGACAAAATATGAGGAAGATCCATGATATATATATAGTTGCAAAGAGCAAAATATTTACGATATTTGAAATTAGAAGTGAAGTAGGCCCAAAGTTTAATATTACACACATTTATGCTTTTCTTTTTCGCTCATTCTTCCACATCAATTTTCGAAAATTTTACGAAGAAATCATTATATGAACTTGCAAATTATACTTAAAAAAATTTCAAATGAAGTCATTATTCTTACTGTTGTTTGACCTTGAAAGTTTAGGCTTCCTTTCTAAACAATATATATATATTTTTCCTTTATATGCAGAATTGGTGGAGTTCACCATAGATGTGACAAAGAAACAAGTGATTGTAAAAGGAAATGCCAATTGCCAAATGCCAAAGCAAGAATGA

mRNA sequence

ATGGAGCAATCTTTGTTATTTTCTGAGAATCTAACACCCCCTTCTTTTCAGGTGATTGTTATAAATGCAAATATGAGTTGCAATGGATGTCGCCAAAGAGTTTCTCAAATTGTCTCTAAAATGTCAGAATTGGTGGAGTTCACCATAGATGTGACAAAGAAACAAGTGATTGTAAAAGGAAATGCCAATTGCCAAATGCCAAAGCAAGAATGA

Coding sequence (CDS)

ATGGAGCAATCTTTGTTATTTTCTGAGAATCTAACACCCCCTTCTTTTCAGGTGATTGTTATAAATGCAAATATGAGTTGCAATGGATGTCGCCAAAGAGTTTCTCAAATTGTCTCTAAAATGTCAGAATTGGTGGAGTTCACCATAGATGTGACAAAGAAACAAGTGATTGTAAAAGGAAATGCCAATTGCCAAATGCCAAAGCAAGAATGA

Protein sequence

MEQSLLFSENLTPPSFQVIVINANMSCNGCRQRVSQIVSKMSELVEFTIDVTKKQVIVKGNANCQMPKQE

Homology

[Close]

BLAST of ClCG10G002750 vs. NCBI nr
Match: XP_038903445.1 (copper transport protein ATX1 [Benincasa hispida])

HSP 1 Score: 121.7 bits (304), Expect = 2.5e-24
Identity = 61/70 (87.14%), Postives = 63/70 (90.00%), Query Frame = 0


Query: 1  MEQSLLFSENLTPPSFQVIVINANMSCNGCRQRVSQIVSKMSELVEFTIDVTKKQVIVKG 60
          MEQSLLFSENLTPPSFQVIVINANMSC GCRQRVSQIV KMSELVEFTIDVTK+QVIVKG
Sbjct: 1  MEQSLLFSENLTPPSFQVIVINANMSCKGCRQRVSQIVCKMSELVEFTIDVTKRQVIVKG 60

Query: 61 NANCQMPKQE 71
          N  CQ   +E
Sbjct: 61 NVKCQSKNEE 70

[Close]

BLAST of ClCG10G002750 vs. NCBI nr
Match: KGN55871.1 (hypothetical protein Csa_010412 [Cucumis sativus])

HSP 1 Score: 120.2 bits (300), Expect = 7.3e-24
Identity = 60/70 (85.71%), Postives = 62/70 (88.57%), Query Frame = 0


Query: 1  MEQSLLFSENLTPPSFQVIVINANMSCNGCRQRVSQIVSKMSELVEFTIDVTKKQVIVKG 60
          M+QSLLFSENLTPP F+VIVINANMSCNGCRQRVSQIVSKMSEL E TIDVTKKQVIVKG
Sbjct: 2  MKQSLLFSENLTPPKFEVIVINANMSCNGCRQRVSQIVSKMSELAELTIDVTKKQVIVKG 61

Query: 61 NANCQMPKQE 71
          N  CQ   QE
Sbjct: 62 NVKCQSKNQE 71

[Close]

BLAST of ClCG10G002750 vs. NCBI nr
Match: XP_023553457.1 (uncharacterized protein LOC111810871 [Cucurbita pepo subsp. pepo])

HSP 1 Score: 115.5 bits (288), Expect = 1.8e-22
Identity = 57/65 (87.69%), Postives = 62/65 (95.38%), Query Frame = 0


Query: 1  MEQSLLFSENLTPPSFQVIVINANMSCNGCRQRVSQIVSKMSELVEFTIDVTKKQVIVKG 60
          MEQSLLFSE+LTPPSFQVIVINANMSCNGCRQRVSQ++SK+SELVEFTIDVTKKQV+VKG
Sbjct: 1  MEQSLLFSEDLTPPSFQVIVINANMSCNGCRQRVSQLLSKISELVEFTIDVTKKQVVVKG 60

Query: 61 NANCQ 66
          N   Q
Sbjct: 61 NVKFQ 65

[Close]

BLAST of ClCG10G002750 vs. NCBI nr
Match: XP_022965234.1 (uncharacterized protein LOC111465148 isoform X1 [Cucurbita maxima])

HSP 1 Score: 115.5 bits (288), Expect = 1.8e-22
Identity = 57/65 (87.69%), Postives = 62/65 (95.38%), Query Frame = 0


Query: 1  MEQSLLFSENLTPPSFQVIVINANMSCNGCRQRVSQIVSKMSELVEFTIDVTKKQVIVKG 60
          MEQSLLFSE+LTPPSFQVIVINANMSCNGCRQRVSQ++SK+SELVEFTIDVTKKQV+VKG
Sbjct: 1  MEQSLLFSEDLTPPSFQVIVINANMSCNGCRQRVSQLLSKISELVEFTIDVTKKQVVVKG 60

Query: 61 NANCQ 66
          N   Q
Sbjct: 61 NVKFQ 65

[Close]

BLAST of ClCG10G002750 vs. NCBI nr
Match: XP_022154033.1 (uncharacterized protein LOC111021388 isoform X1 [Momordica charantia])

HSP 1 Score: 106.3 bits (264), Expect = 1.1e-19
Identity = 53/70 (75.71%), Postives = 59/70 (84.29%), Query Frame = 0


Query: 1  MEQSLLFSENLTPPSFQVIVINANMSCNGCRQRVSQIVSKMSELVEFTIDVTKKQVIVKG 60
          M+QSLLF ENLTPP FQVIVINANM CN CRQRVSQ++SKMSEL+E TIDVTK QV+VKG
Sbjct: 1  MKQSLLFYENLTPPLFQVIVINANMGCNRCRQRVSQLLSKMSELIEVTIDVTKNQVVVKG 60

Query: 61 NANCQMPKQE 71
          N   Q  K+E
Sbjct: 61 NIKFQSKKEE 70

[Close]

BLAST of ClCG10G002750 vs. ExPASy TrEMBL
Match: A0A0A0L6W5 (HMA domain-containing protein OS=Cucumis sativus OX=3659 GN=Csa_3G020590 PE=4 SV=1)

HSP 1 Score: 120.2 bits (300), Expect = 3.5e-24
Identity = 60/70 (85.71%), Postives = 62/70 (88.57%), Query Frame = 0


Query: 1  MEQSLLFSENLTPPSFQVIVINANMSCNGCRQRVSQIVSKMSELVEFTIDVTKKQVIVKG 60
          M+QSLLFSENLTPP F+VIVINANMSCNGCRQRVSQIVSKMSEL E TIDVTKKQVIVKG
Sbjct: 2  MKQSLLFSENLTPPKFEVIVINANMSCNGCRQRVSQIVSKMSELAELTIDVTKKQVIVKG 61

Query: 61 NANCQMPKQE 71
          N  CQ   QE
Sbjct: 62 NVKCQSKNQE 71

[Close]

BLAST of ClCG10G002750 vs. ExPASy TrEMBL
Match: A0A6J1HQF9 (uncharacterized protein LOC111465148 isoform X1 OS=Cucurbita maxima OX=3661 GN=LOC111465148 PE=4 SV=1)

HSP 1 Score: 115.5 bits (288), Expect = 8.7e-23
Identity = 57/65 (87.69%), Postives = 62/65 (95.38%), Query Frame = 0


Query: 1  MEQSLLFSENLTPPSFQVIVINANMSCNGCRQRVSQIVSKMSELVEFTIDVTKKQVIVKG 60
          MEQSLLFSE+LTPPSFQVIVINANMSCNGCRQRVSQ++SK+SELVEFTIDVTKKQV+VKG
Sbjct: 1  MEQSLLFSEDLTPPSFQVIVINANMSCNGCRQRVSQLLSKISELVEFTIDVTKKQVVVKG 60

Query: 61 NANCQ 66
          N   Q
Sbjct: 61 NVKFQ 65

[Close]

BLAST of ClCG10G002750 vs. ExPASy TrEMBL
Match: A0A6J1HN60 (uncharacterized protein LOC111465148 isoform X2 OS=Cucurbita maxima OX=3661 GN=LOC111465148 PE=4 SV=1)

HSP 1 Score: 106.3 bits (264), Expect = 5.3e-20
Identity = 55/65 (84.62%), Postives = 60/65 (92.31%), Query Frame = 0


Query: 1  MEQSLLFSENLTPPSFQVIVINANMSCNGCRQRVSQIVSKMSELVEFTIDVTKKQVIVKG 60
          MEQSLLFSE+LTPPS  VIVINANMSCNGCRQRVSQ++SK+SELVEFTIDVTKKQV+VKG
Sbjct: 1  MEQSLLFSEDLTPPS--VIVINANMSCNGCRQRVSQLLSKISELVEFTIDVTKKQVVVKG 60

Query: 61 NANCQ 66
          N   Q
Sbjct: 61 NVKFQ 63

[Close]

BLAST of ClCG10G002750 vs. ExPASy TrEMBL
Match: A0A6J1DJ58 (uncharacterized protein LOC111021388 isoform X1 OS=Momordica charantia OX=3673 GN=LOC111021388 PE=4 SV=1)

HSP 1 Score: 106.3 bits (264), Expect = 5.3e-20
Identity = 53/70 (75.71%), Postives = 59/70 (84.29%), Query Frame = 0


Query: 1  MEQSLLFSENLTPPSFQVIVINANMSCNGCRQRVSQIVSKMSELVEFTIDVTKKQVIVKG 60
          M+QSLLF ENLTPP FQVIVINANM CN CRQRVSQ++SKMSEL+E TIDVTK QV+VKG
Sbjct: 1  MKQSLLFYENLTPPLFQVIVINANMGCNRCRQRVSQLLSKMSELIEVTIDVTKNQVVVKG 60

Query: 61 NANCQMPKQE 71
          N   Q  K+E
Sbjct: 61 NIKFQSKKEE 70

[Close]

BLAST of ClCG10G002750 vs. ExPASy TrEMBL
Match: A0A6J1DKV3 (uncharacterized protein LOC111021388 isoform X2 OS=Momordica charantia OX=3673 GN=LOC111021388 PE=4 SV=1)

HSP 1 Score: 97.1 bits (240), Expect = 3.2e-17
Identity = 51/70 (72.86%), Postives = 57/70 (81.43%), Query Frame = 0


Query: 1  MEQSLLFSENLTPPSFQVIVINANMSCNGCRQRVSQIVSKMSELVEFTIDVTKKQVIVKG 60
          M+QSLLF ENLTPP   VIVINANM CN CRQRVSQ++SKMSEL+E TIDVTK QV+VKG
Sbjct: 1  MKQSLLFYENLTPP--LVIVINANMGCNRCRQRVSQLLSKMSELIEVTIDVTKNQVVVKG 60

Query: 61 NANCQMPKQE 71
          N   Q  K+E
Sbjct: 61 NIKFQSKKEE 68

[Close]

BLAST of ClCG10G002750 vs. TAIR 10
Match: AT2G35730.1 (Heavy metal transport/detoxification superfamily protein )

HSP 1 Score: 60.1 bits (144), Expect = 8.3e-10
Identity = 28/59 (47.46%), Postives = 44/59 (74.58%), Query Frame = 0


Query: 3  QSLLFSENLTPPSFQVIVINANMSCNGCRQRVSQIVSKMSELVEFTIDVTKKQVIVKGN 62
          ++L+  E L+ PSFQVI INA++ C  C+ RVS+IVSKM+ + E+ +D+ KK V+ +G+
Sbjct: 9  RTLMLCEKLSLPSFQVIEINADVGCVACQDRVSKIVSKMTGIEEYVVDLKKKLVMARGD 67

[Close]

BLAST of ClCG10G002750 vs. TAIR 10
Match: AT3G56240.1 (copper chaperone )

HSP 1 Score: 42.4 bits (98), Expect = 1.8e-04
Identity = 18/45 (40.00%), Postives = 29/45 (64.44%), Query Frame = 0


Query: 17 QVIVINANMSCNGCRQRVSQIVSKMSELVEFTIDVTKKQVIVKGN 62
          Q +V+   MSC GC   V++++ KM  +  F ID+ +++V VKGN
Sbjct: 3  QTVVLKVGMSCQGCVGAVNRVLGKMEGVESFDIDIKEQKVTVKGN 47

The following BLAST results are available for this feature:

BLAST of ClCG10G002750 vs. NCBI nr


Match Name	E-value	Identity	Description
XP_038903445.1	2.5e-24	87.14	copper transport protein ATX1 [Benincasa hispida]	[more]
KGN55871.1	7.3e-24	85.71	hypothetical protein Csa_010412 [Cucumis sativus]	[more]
XP_023553457.1	1.8e-22	87.69	uncharacterized protein LOC111810871 [Cucurbita pepo subsp. pepo]	[more]
XP_022965234.1	1.8e-22	87.69	uncharacterized protein LOC111465148 isoform X1 [Cucurbita maxima]	[more]
XP_022154033.1	1.1e-19	75.71	uncharacterized protein LOC111021388 isoform X1 [Momordica charantia]	[more]

BLAST of ClCG10G002750 vs. NCBI nr


Match Name	E-value	Identity	Description

BLAST of ClCG10G002750 vs. ExPASy TrEMBL


Match Name	E-value	Identity	Description
A0A0A0L6W5	3.5e-24	85.71	HMA domain-containing protein OS=Cucumis sativus OX=3659 GN=Csa_3G020590 PE=4 SV...	[more]
A0A6J1HQF9	8.7e-23	87.69	uncharacterized protein LOC111465148 isoform X1 OS=Cucurbita maxima OX=3661 GN=L...	[more]
A0A6J1HN60	5.3e-20	84.62	uncharacterized protein LOC111465148 isoform X2 OS=Cucurbita maxima OX=3661 GN=L...	[more]
A0A6J1DJ58	5.3e-20	75.71	uncharacterized protein LOC111021388 isoform X1 OS=Momordica charantia OX=3673 G...	[more]
A0A6J1DKV3	3.2e-17	72.86	uncharacterized protein LOC111021388 isoform X2 OS=Momordica charantia OX=3673 G...	[more]

BLAST of ClCG10G002750 vs. TAIR 10


Match Name	E-value	Identity	Description
AT2G35730.1	8.3e-10	47.46	Heavy metal transport/detoxification superfamily protein	[more]
AT3G56240.1	1.8e-04	40.00	copper chaperone	[more]

InterPro

Analysis Name: InterPro Annotations of Watermelon (Charleston Gray) v2.5
Date Performed: 2022-01-31


IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
IPR006121	Heavy metal-associated domain, HMA	PFAM	PF00403	HMA	coord: 24..62 e-value: 5.8E-7 score: 29.8
IPR006121	Heavy metal-associated domain, HMA	PROSITE	PS50846	HMA_2	coord: 17..70 score: 8.542343
IPR006121	Heavy metal-associated domain, HMA	CDD	cd00371	HMA	coord: 23..63 e-value: 0.00287285 score: 30.6517
None	No IPR available	GENE3D	3.30.70.100		coord: 15..70 e-value: 1.5E-8 score: 36.7
IPR017969	Heavy-metal-associated, conserved site	PROSITE	PS01047	HMA_1	coord: 22..51
IPR036163	Heavy metal-associated domain superfamily	SUPERFAMILY	55008	HMA, heavy metal-associated domain	coord: 15..63

Relationships

The following mRNA feature(s) are a part of this gene:


Feature Name	Unique Name	Type
ClCG10G002750.2	ClCG10G002750.2	mRNA

GO Annotation

GO Assignments

This gene is annotated with the following GO terms.

Category	Term Accession	Term Name
molecular_function	GO:0046872	metal ion binding