ClCG09G007850 (gene) Watermelon (Charleston Gray) v2.5

Overview
NameClCG09G007850
Typegene
OrganismCitrullus lanatus subsp. vulgaris cv. Charleston Gray (Watermelon (Charleston Gray) v2.5)
Description2-oxoglutarate (2OG) and Fe(II)-dependent oxygenase superfamily protein
LocationCG_Chr09: 7173838 .. 7178119 (+)
RNA-Seq ExpressionClCG09G007850
SyntenyClCG09G007850
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: exonCDSpolypeptide
Hold the cursor over a type above to highlight its positions in the sequence below.
ATGTCATCAAAAGAATGGATAGATGAAATACCAGTAATGGACATTGATGCTCATAGCTTTAGCGAATCAGCTATATGTTCCCTACGCAGAGCTTGCAAGGAATGGGGATTCTTCTTCATTCGAAACCATGGCGTTCCTAAGGAGTTTTTCAGAAAACTCATCTCCGACGCCGACCGACGACTGTTTACTTTTCCCGACGAGACGAAGCGGAAACTGAAAGTGGGAGCTTCCTCTTACACCCCTAGATTTGTGGTGTCACCATCCCTGGAGAGCTTCAAGGTTTTAGGCCCAAACTTCTCTGCTTCTGCTATTGCTCATGGTTTTACTGACTCCTTATTTGGCCACCAAGCTACTCAATTCAGGTCACCTTTTCTTTCCTTTTTTTTCTTTTCGGGTTTCGAATCGGAAAGAATTGTATGAATGAGACGGCTACAATATGATTGTCAAAATAAACTAACAGTGCCATATGAATATGAAATATAGAGGTCTCCTAATAAGTAATAATTAACTACTAAATAGAGATTTTAATTATGTCTTTTTGGTGGAAAGATTTGTCCTTGAGTTATATGTCTTTTTTTTATTTTTTTAGAAATTATCTGAAGAGGTAGACTGATACAATTTGAAGATAAAATCGTTGGATATGCAAGAGAGAGACTAAAAATTGTGATTTCACTATAAATAAATCCAACAGTTTGGAGGGTAGAGTGGTCAGATTGAATTATTTTTCCACTTTTTAGGGTGTTTGTTTTGTGGTTCCATTACAAATTTGAAGATATTGTGTTTGTTTAGTTTTCAAACCTTAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAACAAAAAAAAAAAAAAAAAAAANNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAATTTGTAAGTTGTCGTAATGATTTGACCGTCATGATTATTCTTTGTTTGTCTACACTACAATGCCATGAAATAAACAATTATGATAATTTTTTTTTGAAGTGACATTGATACTATATTCTTTCCTTAATAATCGATTCATAGTCTAAAAAAAGAGTCATGAAATGTGGTTGACAATTTTAATTCCTTAAATGCTACACGACATGCTAACTTAGTAATTAAAATTCAAAGGTAGGTTAAGCAACAACTACATTAAACATTATTTTCTTATCAAAGTGAGTATTAACTCAGCGGTGAGTAGCATGTACTAAGTTGGAGGTTCAAATCTCTCTACCCTATTTGTTGTACTGGAGAAGAAAAAAAATATTATTTTCTTCATTATTTAATGATCGTTCCATCATCATCTTATTTATTATATATCTAGACATGGATGTGTGTTGTAAGTTGAATTTATTTGCAGGAATCTTCTAGACGAATATGGAAGCAAAATGATAGAAATATCAAAAAGAGTAGTACAACTATTACTAAAAACATTGGGAGATGATATTGAAACCAAATTCTATGATTCTGAGTTCAGTAATTGCTATGGCTATATAAGGATAAACAGATACTCTCCTCAAGACTTGAACGACGAAGAAGAAATGGATGCATTCAAGCAGCACACTGACATCAGCTACGTGACGATCTTGTTCCAAGACGATGTTGGGGGACTACAGATGAGATCGAAAGGAGGCGAGTGGTTTGATGTAAAGCCGAGCGAGGACGGTGATCTTTTGGTGAACATTGGGGATTTTATGGAGGGTTGGAGCAATGGGAGGGTGAAATCAGCTGAACATAGAGTTGTTTTGAGGAAAAATAATGTGAAGACGAGATTTTCTGTGGCTTTCTTTTTGACAAATGAAGATGATGATAATAAGGAAATATATGCTCCAAGTGAGGTTGTTGGAGAAGGGAAATCAAGGCTTTACCTCCCATTTTCAACTAAAGAATATAGGTTATTTAGGGAAAAGAATTATGGGAGAATTGGAGCCACACTTAAAGATTTTACTGGAATATAA

mRNA sequence

ATGTCATCAAAAGAATGGATAGATGAAATACCAGTAATGGACATTGATGCTCATAGCTTTAGCGAATCAGCTATATGTTCCCTACGCAGAGCTTGCAAGGAATGGGGATTCTTCTTCATTCGAAACCATGGCGTTCCTAAGGAGTTTTTCAGAAAACTCATCTCCGACGCCGACCGACGACTGTTTACTTTTCCCGACGAGACGAAGCGGAAACTGAAAGTGGGAGCTTCCTCTTACACCCCTAGATTTGTGGTGTCACCATCCCTGGAGAGCTTCAAGGTTTTAGGCCCAAACTTCTCTGCTTCTGCTATTGCTCATGGTTTTACTGACTCCTTATTTGGCCACCAAGCTACTCAATTCAGGAATCTTCTAGACGAATATGGAAGCAAAATGATAGAAATATCAAAAAGAGTAGTACAACTATTACTAAAAACATTGGGAGATGATATTGAAACCAAATTCTATGATTCTGAGTTCAGTAATTGCTATGGCTATATAAGGATAAACAGATACTCTCCTCAAGACTTGAACGACGAAGAAGAAATGGATGCATTCAAGCAGCACACTGACATCAGCTACGTGACGATCTTGTTCCAAGACGATGTTGGGGGACTACAGATGAGATCGAAAGGAGGCGAGTGGTTTGATGTAAAGCCGAGCGAGGACGGTGATCTTTTGGTGAACATTGGGGATTTTATGGAGGGTTGGAGCAATGGGAGGGTGAAATCAGCTGAACATAGAGTTGTTTTGAGGAAAAATAATGTGAAGACGAGATTTTCTGTGGCTTTCTTTTTGACAAATGAAGATGATGATAATAAGGAAATATATGCTCCAAGTGAGGTTGTTGGAGAAGGGAAATCAAGGCTTTACCTCCCATTTTCAACTAAAGAATATAGGTTATTTAGGGAAAAGAATTATGGGAGAATTGGAGCCACACTTAAAGATTTTACTGGAATATAA

Coding sequence (CDS)

ATGTCATCAAAAGAATGGATAGATGAAATACCAGTAATGGACATTGATGCTCATAGCTTTAGCGAATCAGCTATATGTTCCCTACGCAGAGCTTGCAAGGAATGGGGATTCTTCTTCATTCGAAACCATGGCGTTCCTAAGGAGTTTTTCAGAAAACTCATCTCCGACGCCGACCGACGACTGTTTACTTTTCCCGACGAGACGAAGCGGAAACTGAAAGTGGGAGCTTCCTCTTACACCCCTAGATTTGTGGTGTCACCATCCCTGGAGAGCTTCAAGGTTTTAGGCCCAAACTTCTCTGCTTCTGCTATTGCTCATGGTTTTACTGACTCCTTATTTGGCCACCAAGCTACTCAATTCAGGAATCTTCTAGACGAATATGGAAGCAAAATGATAGAAATATCAAAAAGAGTAGTACAACTATTACTAAAAACATTGGGAGATGATATTGAAACCAAATTCTATGATTCTGAGTTCAGTAATTGCTATGGCTATATAAGGATAAACAGATACTCTCCTCAAGACTTGAACGACGAAGAAGAAATGGATGCATTCAAGCAGCACACTGACATCAGCTACGTGACGATCTTGTTCCAAGACGATGTTGGGGGACTACAGATGAGATCGAAAGGAGGCGAGTGGTTTGATGTAAAGCCGAGCGAGGACGGTGATCTTTTGGTGAACATTGGGGATTTTATGGAGGGTTGGAGCAATGGGAGGGTGAAATCAGCTGAACATAGAGTTGTTTTGAGGAAAAATAATGTGAAGACGAGATTTTCTGTGGCTTTCTTTTTGACAAATGAAGATGATGATAATAAGGAAATATATGCTCCAAGTGAGGTTGTTGGAGAAGGGAAATCAAGGCTTTACCTCCCATTTTCAACTAAAGAATATAGGTTATTTAGGGAAAAGAATTATGGGAGAATTGGAGCCACACTTAAAGATTTTACTGGAATATAA

Protein sequence

MSSKEWIDEIPVMDIDAHSFSESAICSLRRACKEWGFFFIRNHGVPKEFFRKLISDADRRLFTFPDETKRKLKVGASSYTPRFVVSPSLESFKVLGPNFSASAIAHGFTDSLFGHQATQFRNLLDEYGSKMIEISKRVVQLLLKTLGDDIETKFYDSEFSNCYGYIRINRYSPQDLNDEEEMDAFKQHTDISYVTILFQDDVGGLQMRSKGGEWFDVKPSEDGDLLVNIGDFMEGWSNGRVKSAEHRVVLRKNNVKTRFSVAFFLTNEDDDNKEIYAPSEVVGEGKSRLYLPFSTKEYRLFREKNYGRIGATLKDFTGI
Homology
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BLAST of ClCG09G007850 vs. NCBI nr
Match: XP_038899419.1 (gibberellin 20-oxidase-like protein [Benincasa hispida])

HSP 1 Score: 549.7 bits (1415), Expect = 1.7e-152
Identity = 266/320 (83.12%), Postives = 294/320 (91.88%), Query Frame = 0 

Query: 1   MSSKEWIDEIPVMDIDAHSFSESAICSLRRACKEWGFFFIRNHGVPKEFFRKLISDADRR 60
           MSSKEWI+EIPVMDI +HSFS S I SL +ACKEWGFFFIRNHG+PKEF+ KLISDADRR
Sbjct: 1   MSSKEWIEEIPVMDIYSHSFSNSDISSLSKACKEWGFFFIRNHGIPKEFYGKLISDADRR 60

Query: 61  LFTFPDETKRKLKVGASSYTPRFVVSPSLESFKVLGPNFSASAIAHGFTDSLFGHQATQF 120
           LF+FPDETKRK+KVG SSYTPRFVVSPS+ESFKVLGPNFSASA AHGFTDS+FG QAT+F
Sbjct: 61  LFSFPDETKRKMKVGVSSYTPRFVVSPSMESFKVLGPNFSASAAAHGFTDSIFGRQATEF 120

Query: 121 RNLLDEYGSKMIEISKRVVQLLLKTLGDDIETKFYDSEFSNCYGYIRINRYSPQDLNDEE 180
           RNLLDEYGSKM E+SKRVVQLLLKTLG+D+E KFY+SEF NC+GY+RIN Y+ QDLNDEE
Sbjct: 121 RNLLDEYGSKMEEVSKRVVQLLLKTLGEDLENKFYESEFGNCHGYLRINSYTLQDLNDEE 180

Query: 181 EMDAFKQHTDISYVTILFQDDVGGLQMRSKGGEWFDVKPSEDGDLLVNIGDFMEGWSNGR 240
           E+  FK+HTD+SYVTILFQDDVGGLQMRSKGGEWFDVKPS+DG+LLVNIGDFME WSNG+
Sbjct: 181 EVVGFKKHTDLSYVTILFQDDVGGLQMRSKGGEWFDVKPSKDGNLLVNIGDFMEAWSNGK 240

Query: 241 VKSAEHRVVLRKN-NVKTRFSVAFFLTNEDDDNKEIYAPSEVVGEGKSRLYLPFSTKEYR 300
           V+SAEHRVVLRK  NVK RFSVAFFLT ED D +EIYAP+EVVGEGKSRLYLPFST+EYR
Sbjct: 241 VRSAEHRVVLRKKINVKKRFSVAFFLTYEDGD-REIYAPTEVVGEGKSRLYLPFSTQEYR 300

Query: 301 LFREKNYGRIGATLKDFTGI 320
           LFREKNY +I ATLKDF GI
Sbjct: 301 LFREKNYEKIDATLKDFAGI 319

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BLAST of ClCG09G007850 vs. NCBI nr
Match: XP_022991453.1 (gibberellin 20-oxidase-like protein isoform X1 [Cucurbita maxima])

HSP 1 Score: 444.1 bits (1141), Expect = 1.0e-120
Identity = 220/311 (70.74%), Postives = 263/311 (84.57%), Query Frame = 0 

Query: 7   IDEIPVMDIDAHSFSESAICSLRRACKEWGFFFIRNHGVPKEFFRKLISDADRRLFTFPD 66
           I E+PV+DI + S +ESA  SL RAC E+GFFFIRNHG+ KE ++KLIS AD +LF+ P+
Sbjct: 3   IVEVPVIDISSQSLTESAASSLLRACNEFGFFFIRNHGLSKELYQKLISGAD-QLFSSPE 62

Query: 67  ETKRKLKVGASSYTPRFVVSPSLESFKVLGPNFSASAIAHGFTDSLFGHQATQFRNLLDE 126
           ETK+KL +GASSY PRFV+SP  ESFKV GPNFSASA A GFT SLFGHQAT+F NLLDE
Sbjct: 63  ETKQKLTIGASSYIPRFVLSPFFESFKVSGPNFSASANAQGFTQSLFGHQATEFSNLLDE 122

Query: 127 YGSKMIEISKRVVQLLLKTLGDDIETKFYDSEFSNCYGYIRINRYSPQDLN-DEEEMDAF 186
           YGSKM E+SKRVV+LLL  LGD+IE KFYDSEFSNC  YIRIN ++P+ +N +EEE++ F
Sbjct: 123 YGSKMKEVSKRVVKLLLNILGDNIENKFYDSEFSNCNAYIRINGFTPRGMNEEEEELEVF 182

Query: 187 KQHTDISYVTILFQDDVGGLQMRSKGGEWFDVKPSEDGDLLVNIGDFMEGWSNGRVKSAE 246
           K+HTD+SYVTI+FQ+D+ GLQMR KGG+W DV+PSED +L+VNIGDFME WSNGR++SA 
Sbjct: 183 KKHTDLSYVTIVFQNDIRGLQMRLKGGDWMDVRPSEDNNLVVNIGDFMEAWSNGRLRSAV 242

Query: 247 HRVVLRKNNVKTRFSVAFFLTNEDDDNKEIYAPSEVVGEGKSRLYLPFSTKEYRLFREKN 306
           HRVVLRKNN+K RFS+AFFLT +DDD KEIYAPSE+VGEGKSRLY PFST++YRLF+EKN
Sbjct: 243 HRVVLRKNNMK-RFSMAFFLTPKDDD-KEIYAPSEIVGEGKSRLYRPFSTQQYRLFKEKN 302

Query: 307 YGRIGATLKDF 317
               G  LKDF
Sbjct: 303 DVNAGYQLKDF 310

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BLAST of ClCG09G007850 vs. NCBI nr
Match: XP_022953360.1 (gibberellin 20-oxidase-like protein [Cucurbita moschata])

HSP 1 Score: 441.8 bits (1135), Expect = 5.0e-120
Identity = 211/313 (67.41%), Postives = 262/313 (83.71%), Query Frame = 0 

Query: 7   IDEIPVMDIDAHSFSESAICSLRRACKEWGFFFIRNHGVPKEFFRKLISDADRRLFTFPD 66
           I+E+PV+DI + + +ES   SL RAC E GFFFI+NHG+ KEF++KLIS  D +LF+ P+
Sbjct: 3   IEEVPVIDISSQTLTESTASSLSRACTELGFFFIKNHGISKEFYQKLISGID-QLFSSPE 62

Query: 67  ETKRKLKVGASSYTPRFVVSPSLESFKVLGPNFSASAIAHGFTDSLFGHQATQFRNLLDE 126
           ETK+KLK G SSYTPRFVVSP LESFK+ GP FSASA+AHGFT S FGHQAT+F NLLDE
Sbjct: 63  ETKQKLKQGVSSYTPRFVVSPFLESFKLSGPKFSASAVAHGFTQSQFGHQATEFSNLLDE 122

Query: 127 YGSKMIEISKRVVQLLLKTLGDDIETKFYDSEFSNCYGYIRINRYSPQDLNDEEEMDAFK 186
           YGSKM E+SK+VV+LLLK LGD+IE KFYD EFSNC  Y+RIN Y+P  +NDEEE++ FK
Sbjct: 123 YGSKMTEVSKKVVKLLLKILGDNIENKFYDPEFSNCTSYLRINSYTPLGMNDEEEVEVFK 182

Query: 187 QHTDISYVTILFQDDVGGLQMRSKGGEWFDVKPSEDGDLLVNIGDFMEGWSNGRVKSAEH 246
           +HTD+SY TI+F +D+ GLQMRSKGG+W +V+PSEDG+L+VNIGD+ME WSNGR++SA H
Sbjct: 183 KHTDLSYATIVFPNDMRGLQMRSKGGDWINVRPSEDGNLVVNIGDYMEAWSNGRLRSALH 242

Query: 247 RVVLRKNNVKTRFSVAFFLTNEDDDNKEIYAPSEVVGEGKSRLYLPFSTKEYRLFREKNY 306
           RVVLRKN  K RFS+A FL+  +D+++EIYAPSEVVGEGKSRLY  FS ++Y+ FRE NY
Sbjct: 243 RVVLRKNITK-RFSIA-FLSTPEDESREIYAPSEVVGEGKSRLYRSFSIQQYKHFRENNY 302

Query: 307 GRIGATLKDFTGI 320
            ++GAT+KDF GI
Sbjct: 303 KKVGATIKDFVGI 312

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BLAST of ClCG09G007850 vs. NCBI nr
Match: KAG6576054.1 (Gibberellin 20-oxidase-like protein, partial [Cucurbita argyrosperma subsp. sororia])

HSP 1 Score: 441.4 bits (1134), Expect = 6.5e-120
Identity = 211/313 (67.41%), Postives = 262/313 (83.71%), Query Frame = 0 

Query: 7   IDEIPVMDIDAHSFSESAICSLRRACKEWGFFFIRNHGVPKEFFRKLISDADRRLFTFPD 66
           I+E+PV+DI + S +ES   SL RAC E GFFFI+NHG+ KEF++KLIS  D +LF+ P+
Sbjct: 3   IEEVPVIDISSQSLTESTASSLTRACTELGFFFIKNHGISKEFYQKLISGID-QLFSSPE 62

Query: 67  ETKRKLKVGASSYTPRFVVSPSLESFKVLGPNFSASAIAHGFTDSLFGHQATQFRNLLDE 126
           ETK+KLK G SSYTPRFVVSP LESFK+ GP FSASA+ HGFT S FGHQAT+F NLLDE
Sbjct: 63  ETKQKLKQGVSSYTPRFVVSPFLESFKLSGPKFSASAVTHGFTQSQFGHQATEFSNLLDE 122

Query: 127 YGSKMIEISKRVVQLLLKTLGDDIETKFYDSEFSNCYGYIRINRYSPQDLNDEEEMDAFK 186
           YGSKM E+SK+VV+LLLK LGD+IE KFYD E+SNC  Y+RIN Y+P  +NDEEE++ FK
Sbjct: 123 YGSKMTEVSKKVVKLLLKILGDNIENKFYDPEYSNCTSYLRINGYTPLGMNDEEEVEVFK 182

Query: 187 QHTDISYVTILFQDDVGGLQMRSKGGEWFDVKPSEDGDLLVNIGDFMEGWSNGRVKSAEH 246
           +HTD+SYVTI+F +D+ GLQMRSKGG+W +V+PSEDG+L+VNIGD+ME WSNGR++SA H
Sbjct: 183 KHTDLSYVTIVFPNDMRGLQMRSKGGDWINVRPSEDGNLVVNIGDYMEAWSNGRLRSALH 242

Query: 247 RVVLRKNNVKTRFSVAFFLTNEDDDNKEIYAPSEVVGEGKSRLYLPFSTKEYRLFREKNY 306
           RVVLRKN  K RFS+A FL+  +D+++EIYAPSEVVGEGKSRLY  FS ++Y+ FRE NY
Sbjct: 243 RVVLRKNITK-RFSIA-FLSTPEDESREIYAPSEVVGEGKSRLYRSFSIQQYKHFRENNY 302

Query: 307 GRIGATLKDFTGI 320
            ++GAT+KDF GI
Sbjct: 303 EKVGATIKDFVGI 312

[Close]
BLAST of ClCG09G007850 vs. NCBI nr
Match: XP_022953356.1 (gibberellin 20-oxidase-like protein isoform X1 [Cucurbita moschata])

HSP 1 Score: 440.3 bits (1131), Expect = 1.4e-119
Identity = 221/318 (69.50%), Postives = 263/318 (82.70%), Query Frame = 0 

Query: 7   IDEIPVMDIDAHSFSESAICSLRRACKEWGFFFIRNHGVPKEFFRKLISDADRRLFTFPD 66
           I+EIPV+DI   S +ESA  SL RAC E+GFFFIRNHG+ KE ++KLIS AD +LF+ P+
Sbjct: 3   IEEIPVIDISCQSLTESAASSLLRACNEFGFFFIRNHGLSKELYQKLISGAD-QLFSSPE 62

Query: 67  ETKRKLKVGASSYTPRFVVSPSLESFKVLGPNFSASAIAHGFTDSLFGHQATQFRNLLDE 126
           ETK+KL +GASSYTPRFVVSP  ESFKV GPNFSASA A GFT SLFGHQAT+F NLLDE
Sbjct: 63  ETKQKLTMGASSYTPRFVVSPFFESFKVSGPNFSASAAAQGFTQSLFGHQATEFSNLLDE 122

Query: 127 YGSKMIEISKRVVQLLLKTLGDDIETKFYDSEFSNCYGYIRINRYSPQDLN----DEEEM 186
           YGSKM E+SKRVV+LLL  LGD+IE KFYDSEFSNC  Y+RIN ++P+ +N    +EEE+
Sbjct: 123 YGSKMKEVSKRVVKLLLNILGDNIEDKFYDSEFSNCNAYLRINGFTPRSMNEEEEEEEEL 182

Query: 187 DAFKQHTDISYVTILFQDDVGGLQMRSKGGEWFDV--KPSEDGDLLVNIGDFMEGWSNGR 246
           + FK+HTD+SYVTI+FQ+D+ GLQMR KGG+W DV  +PSED +L+VNIGDFME WSNGR
Sbjct: 183 EVFKKHTDLSYVTIVFQNDIRGLQMRLKGGDWMDVRPRPSEDDNLMVNIGDFMEAWSNGR 242

Query: 247 VKSAEHRVVLRKNNVKTRFSVAFFLTNEDDDNKEIYAPSEVVGEGKSRLYLPFSTKEYRL 306
           ++SA HRVVLRKNN+K RFS+AFFLT EDDD KEIYAPSE+VGEGKSRLY PFST++YRL
Sbjct: 243 LRSAVHRVVLRKNNMK-RFSMAFFLTTEDDD-KEIYAPSEIVGEGKSRLYRPFSTQQYRL 302

Query: 307 FREKNYGRIGATLKDFTG 319
           F+EK+       LKD  G
Sbjct: 303 FKEKSGVNARYALKDIIG 317

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BLAST of ClCG09G007850 vs. ExPASy Swiss-Prot
Match: F4KBY0 (Gibberellin 20-oxidase-like protein OS=Arabidopsis thaliana OX=3702 GN=At5g51310 PE=2 SV=1)

HSP 1 Score: 258.5 bits (659), Expect = 1.0e-67
Identity = 145/331 (43.81%), Postives = 215/331 (64.95%), Query Frame = 0 

Query: 9   EIPVMDIDAHSFSESAICSLRRACKEWGFFFIRNHGVPKEFFRKLISDADRRLFT--FPD 68
           E+PV DI +   SES++ SL+ ACKEWGFF++ NHGV ++ ++KL     RR  T  F  
Sbjct: 4   ELPVFDI-SKPLSESSLTSLQDACKEWGFFYVTNHGVSRDMYKKL-----RRFSTGVFEL 63

Query: 69  ETKRKLKVGASSYTPRFVVSPSLESFKVLGPNFSASAIAHGFTDSLFGHQAT--QFRNLL 128
           E + K+K+GAS+YTPRF+ SP  ES +V GP+F ASA +   +   F  QAT  +F  L+
Sbjct: 64  EDEEKMKMGASNYTPRFIASPFFESLRVSGPDFYASAKS---SVDAFSDQATDEEFSGLM 123

Query: 129 DEYGSKMIEISKRVVQLLLKTLGDDIETKFYDSEFSNCYGYIRINRYS-PQDLNDE---- 188
            EYG KM ++ +++++ +L + GDD+  K+Y+SEF NC+GY RIN Y+ P D  D+    
Sbjct: 124 KEYGEKMTKLCEKIMKAILSSFGDDLHHKYYESEFGNCHGYFRINNYTIPSDQEDDHHNG 183

Query: 189 EEMDAFK---QHTDISYVTILFQDDVGGLQMRSKGG-EWFDVKPSEDGDLLVNIGDFMEG 248
           +E D  +    HTD+S +TI+ QDD+GGLQ+R++ G    D+ P ++  L+VN+GD +  
Sbjct: 184 DEQDLIEGLGMHTDMSCITIVDQDDIGGLQVRTRDGIGLMDINPKDEA-LVVNVGDLLHA 243

Query: 249 WSNGRVKSAEHRVVL-RKNNVKTRFSVAFFLTNEDDDNKEIYAPSEVVG--EGKSRLYLP 308
           W+NGR++S++HRV+L R+  V  RFS+AFF     DD K ++AP EVVG  EG  R++  
Sbjct: 244 WTNGRLRSSQHRVILKRRGFVGNRFSLAFFWC--FDDGKVVFAPDEVVGGCEG-MRVFRS 303

Query: 309 FSTKEYRLFREKN----YGRIGATLKDFTGI 320
           F   +Y  FRE N    + ++G T++DF  I
Sbjct: 304 FKCGDYLRFRESNEKGKFEKVGDTVEDFARI 321

[Close]
BLAST of ClCG09G007850 vs. ExPASy Swiss-Prot
Match: Q9C6I4 (Gibberellin 2-beta-dioxygenase 7 OS=Arabidopsis thaliana OX=3702 GN=GA2OX7 PE=1 SV=1)

HSP 1 Score: 121.7 bits (304), Expect = 1.5e-26
Identity = 84/298 (28.19%), Postives = 146/298 (48.99%), Query Frame = 0 

Query: 9   EIPVMDIDAHSFSES-----AICSLRRACKEWGFFFIRNHGVPKEFFRKLISDADRRLFT 68
           ++PV+D+   +  E       +  +  A KEWGFF I NHG+PK+ F  ++ + +++LF 
Sbjct: 39  KLPVIDLSHLTSGEEVKRKRCVKQMVAAAKEWGFFQIVNHGIPKDVFEMMLLE-EKKLFD 98

Query: 69  FPDETKRKLKVGASSYTPRFVVSPSLESFKVLGPNFSASAIAHGFTD--SLFGHQATQFR 128
            P   K + +    S       +PS  S       +S S   H      S         R
Sbjct: 99  QPFSVKVRERFSDLSKNSYRWGNPSATS----PAQYSVSEAFHIILSEVSRISDDRNNLR 158

Query: 129 NLLDEYGSKMIEISKRVVQLLLKTLGDDIETKFYDSEFSNCYGYIRINRYSPQDLNDEEE 188
            +++ Y  ++  +++ + ++L K +  ++ ++++++ F     ++R+N+Y P       E
Sbjct: 159 TIVETYVQEIARVAQMICEILGKQV--NVSSEYFENIFELENSFLRLNKYHPSVFG--SE 218

Query: 189 MDAFKQHTDISYVTILFQDDVGGLQMRSKGGEWFDVKPSEDGDLLVNIGDFMEGWSNGRV 248
           +     HTD S++TIL QD +GGL++ +  G+W  VKP  +  L VNIGD  +  SNG  
Sbjct: 219 VFGLVPHTDTSFLTILSQDQIGGLELEN-NGQWISVKPCLEA-LTVNIGDMFQALSNGVY 278

Query: 249 KSAEHRVVLRKNNVKTRFSVAFFLTNEDDDNKEIYAPSEVVGEGKSRLYLPFSTKEYR 300
           +S  HRV+   N    R S+AFF+          Y  +E+   G  + Y  FS +EY+
Sbjct: 279 QSVRHRVISPAN--IERMSIAFFVCP--------YLETEIDCFGYPKKYRRFSFREYK 315

[Close]
BLAST of ClCG09G007850 vs. ExPASy Swiss-Prot
Match: O49561 (Gibberellin 2-beta-dioxygenase 8 OS=Arabidopsis thaliana OX=3702 GN=GA2OX8 PE=1 SV=2)

HSP 1 Score: 118.6 bits (296), Expect = 1.3e-25
Identity = 83/263 (31.56%), Postives = 129/263 (49.05%), Query Frame = 0 

Query: 9   EIPVMDID-----AHSFSESAICSLRRACKEWGFFFIRNHGVPKEFFRKLISDADRRLFT 68
           E+PV+D+      A    E    ++ RA +EWGFF + NHG+  +   K+  +   R+F 
Sbjct: 41  ELPVIDVSRLIDGAEEEREKCKEAIARASREWGFFQVINHGISMDVLEKMRQE-QIRVFR 100

Query: 69  FP-DETKRKLKVGASSYTPRFVVSPSLESFKVLGPNFSASAIAHGFTDSLFGHQATQFRN 128
            P D+  +  K  A SY      +PS  S + L     + A     TD       T   +
Sbjct: 101 EPFDKKSKSEKFSAGSYR---WGTPSATSIRQLS---WSEAFHVPMTDISDNKDFTTLSS 160

Query: 129 LLDEYGSKMIEISKRVVQLLLKTLGDDIETKFYDSEFSNCYGYIRINRYSPQDLNDEEEM 188
            ++++ S+   ++  + ++L +  G +  + F+         Y+R+NRY P       E+
Sbjct: 161 TMEKFASESEALAYMLAEVLAEKSGQN--SSFFKENCVRNTCYLRMNRYPP--CPKPSEV 220

Query: 189 DAFKQHTDISYVTILFQDDVGGLQMRSKGGEWFDVKPSEDGDLLVNIGDFMEGWSNGRVK 248
                HTD  ++TIL+QD VGGLQ+  K   W  VKP+    L++NIGD  + WSNG  K
Sbjct: 221 YGLMPHTDSDFLTILYQDQVGGLQL-IKDNRWIAVKPNPKA-LIINIGDLFQAWSNGMYK 280

Query: 249 SAEHRVVLRKNNVKTRFSVAFFL 266
           S EHRV+   N    RFS A+F+
Sbjct: 281 SVEHRVM--TNPKVERFSTAYFM 288

[Close]
BLAST of ClCG09G007850 vs. ExPASy Swiss-Prot
Match: Q9C899 (Feruloyl CoA ortho-hydroxylase 2 OS=Arabidopsis thaliana OX=3702 GN=F6'H2 PE=1 SV=1)

HSP 1 Score: 116.3 bits (290), Expect = 6.3e-25
Identity = 108/319 (33.86%), Postives = 154/319 (48.28%), Query Frame = 0 

Query: 4   KEWIDE-IPVMDI---DAHSFSESAICSLRRACKEWGFFFIRNHGVPKEFFRKLISDADR 63
           KE  DE IPV+DI   D  S S+ A+C    A +EWGFF + NHGV  E    +   A  
Sbjct: 55  KEDSDESIPVIDISNLDEKSVSK-AVCD---AAEEWGFFQVINHGVSMEVLENM-KTATH 114

Query: 64  RLFTFPDETKRKLKVGAS-SYTPRFVVSPSLESFKVLGPNFSASAIAHGFTDSLFGHQAT 123
           R F  P E KRK     S S   RF  S          P+   +     +    F  +A 
Sbjct: 115 RFFGLPVEEKRKFSREKSLSTNVRFGTS--------FSPHAEKALEWKDYLSLFFVSEAE 174

Query: 124 QFRNLLDEYGSKMIEI---SKRVVQLLLKTLGDDIETKFYDSEFSNCY-GYIRIN-RYSP 183
             +   D   S+ +E    +K +V+ LL+ LG+++  K  D    + + G  RIN  Y P
Sbjct: 175 ASQLWPDSCRSETLEYMNETKPLVKKLLRFLGENLNVKELDKTKESFFMGSTRINLNYYP 234

Query: 184 QDLNDEEEMDAFKQHTDISYVTILFQDDVGGLQMRS-KGGEWFDVKPSEDGDLLVNIGDF 243
              N E  +    +H+D+S +TIL QD++GGL +RS   G W  V P   G L++NIGD 
Sbjct: 235 ICPNPELTV-GVGRHSDVSSLTILLQDEIGGLHVRSLTTGRWVHVPPI-SGSLVINIGDA 294

Query: 244 MEGWSNGRVKSAEHRVVLRKNNVKTRFSVAFFLTNEDDDNKEIYAP-SEVVGEGKSRLYL 303
           M+  SNGR KS EHRV+   N    R SV  F++ + +    +  P  EV+  G+  +Y 
Sbjct: 295 MQIMSNGRYKSVEHRVL--ANGSYNRISVPIFVSPKPE---SVIGPLLEVIENGEKPVYK 353

Query: 304 PFSTKEY--RLFREKNYGR 309
                +Y    FR+ + G+
Sbjct: 355 DILYTDYVKHFFRKAHDGK 353

[Close]
BLAST of ClCG09G007850 vs. ExPASy Swiss-Prot
Match: P10967 (1-aminocyclopropane-1-carboxylate oxidase homolog OS=Solanum lycopersicum OX=4081 GN=ACO3 PE=2 SV=1)

HSP 1 Score: 115.9 bits (289), Expect = 8.2e-25
Identity = 88/300 (29.33%), Postives = 148/300 (49.33%), Query Frame = 0 

Query: 7   IDEIPVMDIDAHSFSESAICSLRRACKEWGFFFIRNHGVPKEFFRKLISDADRRLFTFPD 66
           IDE P+         +  +  +R A ++WGFF + NHG+P     + +    R+ F   +
Sbjct: 66  IDEDPIK-------HKEIVDKVRDASEKWGFFQVVNHGIPTSVLDRTL-QGTRQFFEQDN 125

Query: 67  ETKRKLKVGASSYTPRFVVSPSLESFKVLGPNFSASAIAHGFTDSLFGHQATQFRNLLD- 126
           E K++     ++   + V + +L+ +K       +S  A  + D++F + A    +L + 
Sbjct: 126 EVKKQYYTRDTA--KKVVYTSNLDLYK-------SSVPAASWRDTIFCYMAPNPPSLQEF 185

Query: 127 --EYGSKMIEISKRVVQL---LLKTLGDDIE-TKFYDSEFSNCYGYIRINRYSPQDLNDE 186
               G  +I+ SK V +L   LL+ L + +   + Y  ++ +C+       Y P     E
Sbjct: 186 PTPCGESLIDFSKDVKKLGFTLLELLSEGLGLDRSYLKDYMDCFHLFCSCNYYPPCPQPE 245

Query: 187 EEMDAFKQHTDISYVTILFQDDVGGLQMRSKGGEWFDVKPSEDGDLLVNIGDFMEGWSNG 246
             M    QHTDI +VTIL QDD+GGLQ+  +   W DV P+  G L+VNIGDF++  SN 
Sbjct: 246 LTMGTI-QHTDIGFVTILLQDDMGGLQVLHQ-NHWVDVPPT-PGSLVVNIGDFLQLLSND 305

Query: 247 RVKSAEHRVVLRKNNVKTRFSVAFFLTNEDDDNKEIYAP-SEVVGEGKSRLYLPFSTKEY 299
           +  S EHR +   NNV +R S+  F       + ++Y P +E++ E     Y   + K++
Sbjct: 306 KYLSVEHRAI--SNNVGSRMSITCFFGESPYQSSKLYGPITELLSEDNPPKYRATTVKDH 343

[Close]
BLAST of ClCG09G007850 vs. ExPASy TrEMBL
Match: A0A6J1JW96 (gibberellin 20-oxidase-like protein isoform X1 OS=Cucurbita maxima OX=3661 GN=LOC111488064 PE=3 SV=1)

HSP 1 Score: 444.1 bits (1141), Expect = 4.9e-121
Identity = 220/311 (70.74%), Postives = 263/311 (84.57%), Query Frame = 0 

Query: 7   IDEIPVMDIDAHSFSESAICSLRRACKEWGFFFIRNHGVPKEFFRKLISDADRRLFTFPD 66
           I E+PV+DI + S +ESA  SL RAC E+GFFFIRNHG+ KE ++KLIS AD +LF+ P+
Sbjct: 3   IVEVPVIDISSQSLTESAASSLLRACNEFGFFFIRNHGLSKELYQKLISGAD-QLFSSPE 62

Query: 67  ETKRKLKVGASSYTPRFVVSPSLESFKVLGPNFSASAIAHGFTDSLFGHQATQFRNLLDE 126
           ETK+KL +GASSY PRFV+SP  ESFKV GPNFSASA A GFT SLFGHQAT+F NLLDE
Sbjct: 63  ETKQKLTIGASSYIPRFVLSPFFESFKVSGPNFSASANAQGFTQSLFGHQATEFSNLLDE 122

Query: 127 YGSKMIEISKRVVQLLLKTLGDDIETKFYDSEFSNCYGYIRINRYSPQDLN-DEEEMDAF 186
           YGSKM E+SKRVV+LLL  LGD+IE KFYDSEFSNC  YIRIN ++P+ +N +EEE++ F
Sbjct: 123 YGSKMKEVSKRVVKLLLNILGDNIENKFYDSEFSNCNAYIRINGFTPRGMNEEEEELEVF 182

Query: 187 KQHTDISYVTILFQDDVGGLQMRSKGGEWFDVKPSEDGDLLVNIGDFMEGWSNGRVKSAE 246
           K+HTD+SYVTI+FQ+D+ GLQMR KGG+W DV+PSED +L+VNIGDFME WSNGR++SA 
Sbjct: 183 KKHTDLSYVTIVFQNDIRGLQMRLKGGDWMDVRPSEDNNLVVNIGDFMEAWSNGRLRSAV 242

Query: 247 HRVVLRKNNVKTRFSVAFFLTNEDDDNKEIYAPSEVVGEGKSRLYLPFSTKEYRLFREKN 306
           HRVVLRKNN+K RFS+AFFLT +DDD KEIYAPSE+VGEGKSRLY PFST++YRLF+EKN
Sbjct: 243 HRVVLRKNNMK-RFSMAFFLTPKDDD-KEIYAPSEIVGEGKSRLYRPFSTQQYRLFKEKN 302

Query: 307 YGRIGATLKDF 317
               G  LKDF
Sbjct: 303 DVNAGYQLKDF 310

[Close]
BLAST of ClCG09G007850 vs. ExPASy TrEMBL
Match: A0A6J1GPF2 (gibberellin 20-oxidase-like protein OS=Cucurbita moschata OX=3662 GN=LOC111455932 PE=3 SV=1)

HSP 1 Score: 441.8 bits (1135), Expect = 2.4e-120
Identity = 211/313 (67.41%), Postives = 262/313 (83.71%), Query Frame = 0 

Query: 7   IDEIPVMDIDAHSFSESAICSLRRACKEWGFFFIRNHGVPKEFFRKLISDADRRLFTFPD 66
           I+E+PV+DI + + +ES   SL RAC E GFFFI+NHG+ KEF++KLIS  D +LF+ P+
Sbjct: 3   IEEVPVIDISSQTLTESTASSLSRACTELGFFFIKNHGISKEFYQKLISGID-QLFSSPE 62

Query: 67  ETKRKLKVGASSYTPRFVVSPSLESFKVLGPNFSASAIAHGFTDSLFGHQATQFRNLLDE 126
           ETK+KLK G SSYTPRFVVSP LESFK+ GP FSASA+AHGFT S FGHQAT+F NLLDE
Sbjct: 63  ETKQKLKQGVSSYTPRFVVSPFLESFKLSGPKFSASAVAHGFTQSQFGHQATEFSNLLDE 122

Query: 127 YGSKMIEISKRVVQLLLKTLGDDIETKFYDSEFSNCYGYIRINRYSPQDLNDEEEMDAFK 186
           YGSKM E+SK+VV+LLLK LGD+IE KFYD EFSNC  Y+RIN Y+P  +NDEEE++ FK
Sbjct: 123 YGSKMTEVSKKVVKLLLKILGDNIENKFYDPEFSNCTSYLRINSYTPLGMNDEEEVEVFK 182

Query: 187 QHTDISYVTILFQDDVGGLQMRSKGGEWFDVKPSEDGDLLVNIGDFMEGWSNGRVKSAEH 246
           +HTD+SY TI+F +D+ GLQMRSKGG+W +V+PSEDG+L+VNIGD+ME WSNGR++SA H
Sbjct: 183 KHTDLSYATIVFPNDMRGLQMRSKGGDWINVRPSEDGNLVVNIGDYMEAWSNGRLRSALH 242

Query: 247 RVVLRKNNVKTRFSVAFFLTNEDDDNKEIYAPSEVVGEGKSRLYLPFSTKEYRLFREKNY 306
           RVVLRKN  K RFS+A FL+  +D+++EIYAPSEVVGEGKSRLY  FS ++Y+ FRE NY
Sbjct: 243 RVVLRKNITK-RFSIA-FLSTPEDESREIYAPSEVVGEGKSRLYRSFSIQQYKHFRENNY 302

Query: 307 GRIGATLKDFTGI 320
            ++GAT+KDF GI
Sbjct: 303 KKVGATIKDFVGI 312

[Close]
BLAST of ClCG09G007850 vs. ExPASy TrEMBL
Match: A0A6J1GMS4 (gibberellin 20-oxidase-like protein isoform X1 OS=Cucurbita moschata OX=3662 GN=LOC111455930 PE=3 SV=1)

HSP 1 Score: 440.3 bits (1131), Expect = 7.0e-120
Identity = 221/318 (69.50%), Postives = 263/318 (82.70%), Query Frame = 0 

Query: 7   IDEIPVMDIDAHSFSESAICSLRRACKEWGFFFIRNHGVPKEFFRKLISDADRRLFTFPD 66
           I+EIPV+DI   S +ESA  SL RAC E+GFFFIRNHG+ KE ++KLIS AD +LF+ P+
Sbjct: 3   IEEIPVIDISCQSLTESAASSLLRACNEFGFFFIRNHGLSKELYQKLISGAD-QLFSSPE 62

Query: 67  ETKRKLKVGASSYTPRFVVSPSLESFKVLGPNFSASAIAHGFTDSLFGHQATQFRNLLDE 126
           ETK+KL +GASSYTPRFVVSP  ESFKV GPNFSASA A GFT SLFGHQAT+F NLLDE
Sbjct: 63  ETKQKLTMGASSYTPRFVVSPFFESFKVSGPNFSASAAAQGFTQSLFGHQATEFSNLLDE 122

Query: 127 YGSKMIEISKRVVQLLLKTLGDDIETKFYDSEFSNCYGYIRINRYSPQDLN----DEEEM 186
           YGSKM E+SKRVV+LLL  LGD+IE KFYDSEFSNC  Y+RIN ++P+ +N    +EEE+
Sbjct: 123 YGSKMKEVSKRVVKLLLNILGDNIEDKFYDSEFSNCNAYLRINGFTPRSMNEEEEEEEEL 182

Query: 187 DAFKQHTDISYVTILFQDDVGGLQMRSKGGEWFDV--KPSEDGDLLVNIGDFMEGWSNGR 246
           + FK+HTD+SYVTI+FQ+D+ GLQMR KGG+W DV  +PSED +L+VNIGDFME WSNGR
Sbjct: 183 EVFKKHTDLSYVTIVFQNDIRGLQMRLKGGDWMDVRPRPSEDDNLMVNIGDFMEAWSNGR 242

Query: 247 VKSAEHRVVLRKNNVKTRFSVAFFLTNEDDDNKEIYAPSEVVGEGKSRLYLPFSTKEYRL 306
           ++SA HRVVLRKNN+K RFS+AFFLT EDDD KEIYAPSE+VGEGKSRLY PFST++YRL
Sbjct: 243 LRSAVHRVVLRKNNMK-RFSMAFFLTTEDDD-KEIYAPSEIVGEGKSRLYRPFSTQQYRL 302

Query: 307 FREKNYGRIGATLKDFTG 319
           F+EK+       LKD  G
Sbjct: 303 FKEKSGVNARYALKDIIG 317

[Close]
BLAST of ClCG09G007850 vs. ExPASy TrEMBL
Match: A0A6J1JSZ7 (gibberellin 20-oxidase-like protein OS=Cucurbita maxima OX=3661 GN=LOC111488065 PE=3 SV=1)

HSP 1 Score: 435.3 bits (1118), Expect = 2.3e-118
Identity = 211/313 (67.41%), Postives = 258/313 (82.43%), Query Frame = 0 

Query: 7   IDEIPVMDIDAHSFSESAICSLRRACKEWGFFFIRNHGVPKEFFRKLISDADRRLFTFPD 66
           I E+PV+D+   S +ES   SL RAC + GFFFIRNHGV KEF+ KLIS AD  LF+ P+
Sbjct: 3   IKEVPVIDLGCQSLTESTSSSLLRACNKLGFFFIRNHGVSKEFYEKLISGAD-ELFSSPE 62

Query: 67  ETKRKLKVGASSYTPRFVVSPSLESFKVLGPNFSASAIAHGFTDSLFGHQATQFRNLLDE 126
           ETK++LK+G SSYTPRF+VSP  ESF+V GPNFSASA A GFT SLFGH AT+F NLLDE
Sbjct: 63  ETKQQLKMGVSSYTPRFLVSPFFESFRVSGPNFSASAAAQGFTQSLFGHHATEFSNLLDE 122

Query: 127 YGSKMIEISKRVVQLLLKTLGDDIETKFYDSEFSNCYGYIRINRYSPQDLNDEEEMDAFK 186
           YG KM E+SKRVV+LLL  LGD+IE KFYD EFSNC GY+RIN ++P D+N EEE++ FK
Sbjct: 123 YGRKMKEVSKRVVKLLLNVLGDNIENKFYDPEFSNCNGYLRINGFTPLDMNAEEEIEVFK 182

Query: 187 QHTDISYVTILFQDDVGGLQMRSKGGEWFDVKPSEDGDLLVNIGDFMEGWSNGRVKSAEH 246
           +HTD+SYVTI+FQ+D+ GLQMRSKGG+W DV+ SEDG L+VNIGD+ME WSNGR++SA H
Sbjct: 183 KHTDLSYVTIVFQNDIRGLQMRSKGGDWIDVRLSEDGILVVNIGDYMEAWSNGRLRSAVH 242

Query: 247 RVVLRKNNVKTRFSVAFFLTNEDDDNKEIYAPSEVVGEGKSRLYLPFSTKEYRLFREKNY 306
           RVVLRKN +K RFS+A FL+  +D+++EIYAPSEVVGEGKSRLY  FS ++Y+ FRE NY
Sbjct: 243 RVVLRKNIMK-RFSIA-FLSTPEDESREIYAPSEVVGEGKSRLYRSFSVQQYKHFRENNY 302

Query: 307 GRIGATLKDFTGI 320
            ++GAT+KDF GI
Sbjct: 303 EKVGATIKDFVGI 312

[Close]
BLAST of ClCG09G007850 vs. ExPASy TrEMBL
Match: A0A6J1GMT1 (gibberellin 20-oxidase-like protein OS=Cucurbita moschata OX=3662 GN=LOC111455933 PE=3 SV=1)

HSP 1 Score: 426.0 bits (1094), Expect = 1.4e-115
Identity = 204/313 (65.18%), Postives = 254/313 (81.15%), Query Frame = 0 

Query: 7   IDEIPVMDIDAHSFSESAICSLRRACKEWGFFFIRNHGVPKEFFRKLISDADRRLFTFPD 66
           I+E+PV+DI + S +ES   SL RAC E GFFFI+NHG+ KEF++K IS  D  LF+ P+
Sbjct: 3   IEEVPVIDISSQSLTESTASSLFRACTELGFFFIKNHGISKEFYQKFISGID-LLFSSPE 62

Query: 67  ETKRKLKVGASSYTPRFVVSPSLESFKVLGPNFSASAIAHGFTDSLFGHQATQFRNLLDE 126
           ETK++LK G SSYTPRFV+SP LE+FK  GPNFSAS+ A G T S FGHQAT+F NLLDE
Sbjct: 63  ETKQRLKHGVSSYTPRFVISPFLETFKFSGPNFSASSTAQGVTQSQFGHQATEFSNLLDE 122

Query: 127 YGSKMIEISKRVVQLLLKTLGDDIETKFYDSEFSNCYGYIRINRYSPQDLNDEEEMDAFK 186
           YGSKM E+SKR+++LLL  LGD+IE KFYD EFSNC GY+RIN Y+P  +ND+EE++ FK
Sbjct: 123 YGSKMAEVSKRILKLLLNILGDNIENKFYDPEFSNCNGYLRINGYTPLGMNDDEEVEVFK 182

Query: 187 QHTDISYVTILFQDDVGGLQMRSKGGEWFDVKPSEDGDLLVNIGDFMEGWSNGRVKSAEH 246
           +HTD+SYVTI+F +D+ GLQMRSKGG+W D  PSED  L+VNIGD +E WSNGR++SA H
Sbjct: 183 KHTDVSYVTIVFPNDIRGLQMRSKGGDWIDAMPSEDDTLVVNIGDLLEAWSNGRLRSAIH 242

Query: 247 RVVLRKNNVKTRFSVAFFLTNEDDDNKEIYAPSEVVGEGKSRLYLPFSTKEYRLFREKNY 306
           RVVLRKNN+K RFS+ +  T E D+++EI+APSEVVGEGKSRLY PFST++Y+ FR KNY
Sbjct: 243 RVVLRKNNMK-RFSIVYTSTPE-DESREIHAPSEVVGEGKSRLYRPFSTQQYKDFRNKNY 302

Query: 307 GRIGATLKDFTGI 320
            +IG T+KDF GI
Sbjct: 303 EKIGETIKDFVGI 312

[Close]
BLAST of ClCG09G007850 vs. TAIR 10
Match: AT4G23340.1 (2-oxoglutarate (2OG) and Fe(II)-dependent oxygenase superfamily protein )

HSP 1 Score: 288.9 bits (738), Expect = 5.0e-78
Identity = 156/319 (48.90%), Postives = 211/319 (66.14%), Query Frame = 0 

Query: 9   EIPVMDIDAHSFSESAICSLRRACKEWGFFFIRNHGVPKEFFRKLISDADRRLFTFPDET 68
           ++PV+D+       S + SL  ACKEWGFF++ NHG+ KE F K+ S   R +F  P E+
Sbjct: 9   QLPVLDL-TQPIESSILSSLSEACKEWGFFYVTNHGISKEMFSKICS-LSRDVFKAPLES 68

Query: 69  KRKLKVGASSYTPRFVVSPSLESFKVLGPNFSASAIAHGFTDSLF-GHQATQFRNLLDEY 128
             KLK+G  SYTPR++ SP  ES  V GP+FS SA A    D LF  H   + R  + EY
Sbjct: 69  --KLKLGPISYTPRYIASPYFESLVVSGPDFSDSAKAS--ADVLFQDHHKPELRETMQEY 128

Query: 129 GSKMIEISKRVVQ-LLLKTLGDDIETKFYDSEFSNCYGYIRINRYS-PQDLNDEEEM-DA 188
           G+KM E+SKR+++ LL+ TLGD+   + Y ++FSNC+GY+R+  Y+ P D+  +EE+ + 
Sbjct: 129 GAKMAELSKRLIKILLMMTLGDETGKRLYQTDFSNCHGYLRLVNYTPPHDVEKQEELVEG 188

Query: 189 FKQHTDISYVTILFQDDVGGLQMRSKGGEWFDVKPSEDGDLLVNIGDFMEGWSNGRVKSA 248
              HTD+S +TI++QD VGGLQMRSK G+W D+ P  D  L+VNIGD M+ WSNGR++S+
Sbjct: 189 LGMHTDMSCITIVYQDSVGGLQMRSKEGKWIDINPCND-FLVVNIGDLMQAWSNGRLRSS 248

Query: 249 EHRVVLRKNNVKTRFSVAFFLTNEDDDNKEIYAPSEVVGEGKSRLYLPFSTKEYRLFREK 308
           EHRVVLRK  +  R S+AFFL  ED+  K I AP E+VGEGK R Y  F   EY  FR+ 
Sbjct: 249 EHRVVLRK--LVNRVSLAFFLCFEDE--KVILAPQEIVGEGKQRSYKSFKCSEYLKFRQS 308

Query: 309 N----YGRIGATLKDFTGI 320
           N    + +IG T+KDF G+
Sbjct: 309 NEEGKFEKIGFTVKDFAGL 316

[Close]
BLAST of ClCG09G007850 vs. TAIR 10
Match: AT5G51310.1 (2-oxoglutarate (2OG) and Fe(II)-dependent oxygenase superfamily protein )

HSP 1 Score: 258.5 bits (659), Expect = 7.3e-69
Identity = 145/331 (43.81%), Postives = 215/331 (64.95%), Query Frame = 0 

Query: 9   EIPVMDIDAHSFSESAICSLRRACKEWGFFFIRNHGVPKEFFRKLISDADRRLFT--FPD 68
           E+PV DI +   SES++ SL+ ACKEWGFF++ NHGV ++ ++KL     RR  T  F  
Sbjct: 4   ELPVFDI-SKPLSESSLTSLQDACKEWGFFYVTNHGVSRDMYKKL-----RRFSTGVFEL 63

Query: 69  ETKRKLKVGASSYTPRFVVSPSLESFKVLGPNFSASAIAHGFTDSLFGHQAT--QFRNLL 128
           E + K+K+GAS+YTPRF+ SP  ES +V GP+F ASA +   +   F  QAT  +F  L+
Sbjct: 64  EDEEKMKMGASNYTPRFIASPFFESLRVSGPDFYASAKS---SVDAFSDQATDEEFSGLM 123

Query: 129 DEYGSKMIEISKRVVQLLLKTLGDDIETKFYDSEFSNCYGYIRINRYS-PQDLNDE---- 188
            EYG KM ++ +++++ +L + GDD+  K+Y+SEF NC+GY RIN Y+ P D  D+    
Sbjct: 124 KEYGEKMTKLCEKIMKAILSSFGDDLHHKYYESEFGNCHGYFRINNYTIPSDQEDDHHNG 183

Query: 189 EEMDAFK---QHTDISYVTILFQDDVGGLQMRSKGG-EWFDVKPSEDGDLLVNIGDFMEG 248
           +E D  +    HTD+S +TI+ QDD+GGLQ+R++ G    D+ P ++  L+VN+GD +  
Sbjct: 184 DEQDLIEGLGMHTDMSCITIVDQDDIGGLQVRTRDGIGLMDINPKDEA-LVVNVGDLLHA 243

Query: 249 WSNGRVKSAEHRVVL-RKNNVKTRFSVAFFLTNEDDDNKEIYAPSEVVG--EGKSRLYLP 308
           W+NGR++S++HRV+L R+  V  RFS+AFF     DD K ++AP EVVG  EG  R++  
Sbjct: 244 WTNGRLRSSQHRVILKRRGFVGNRFSLAFFWC--FDDGKVVFAPDEVVGGCEG-MRVFRS 303

Query: 309 FSTKEYRLFREKN----YGRIGATLKDFTGI 320
           F   +Y  FRE N    + ++G T++DF  I
Sbjct: 304 FKCGDYLRFRESNEKGKFEKVGDTVEDFARI 321

[Close]
BLAST of ClCG09G007850 vs. TAIR 10
Match: AT4G23340.2 (2-oxoglutarate (2OG) and Fe(II)-dependent oxygenase superfamily protein )

HSP 1 Score: 203.4 bits (516), Expect = 2.8e-52
Identity = 104/203 (51.23%), Postives = 143/203 (70.44%), Query Frame = 0 

Query: 124 LDEYGSKMIEISKRVVQ-LLLKTLGDDIETKFYDSEFSNCYGYIRINRYS-PQDLNDEEE 183
           + EYG+KM E+SKR+++ LL+ TLGD+   + Y ++FSNC+GY+R+  Y+ P D+  +EE
Sbjct: 1   MQEYGAKMAELSKRLIKILLMMTLGDETGKRLYQTDFSNCHGYLRLVNYTPPHDVEKQEE 60

Query: 184 M-DAFKQHTDISYVTILFQDDVGGLQMRSKGGEWFDVKPSEDGDLLVNIGDFMEGWSNGR 243
           + +    HTD+S +TI++QD VGGLQMRSK G+W D+ P  D  L+VNIGD M+ WSNGR
Sbjct: 61  LVEGLGMHTDMSCITIVYQDSVGGLQMRSKEGKWIDINPCND-FLVVNIGDLMQAWSNGR 120

Query: 244 VKSAEHRVVLRKNNVKTRFSVAFFLTNEDDDNKEIYAPSEVVGEGKSRLYLPFSTKEYRL 303
           ++S+EHRVVLRK  +  R S+AFFL  ED+  K I AP E+VGEGK R Y  F   EY  
Sbjct: 121 LRSSEHRVVLRK--LVNRVSLAFFLCFEDE--KVILAPQEIVGEGKQRSYKSFKCSEYLK 180

Query: 304 FREKN----YGRIGATLKDFTGI 320
           FR+ N    + +IG T+KDF G+
Sbjct: 181 FRQSNEEGKFEKIGFTVKDFAGL 198

[Close]
BLAST of ClCG09G007850 vs. TAIR 10
Match: AT1G50960.1 (gibberellin 2-oxidase 7 )

HSP 1 Score: 121.7 bits (304), Expect = 1.1e-27
Identity = 84/298 (28.19%), Postives = 146/298 (48.99%), Query Frame = 0 

Query: 9   EIPVMDIDAHSFSES-----AICSLRRACKEWGFFFIRNHGVPKEFFRKLISDADRRLFT 68
           ++PV+D+   +  E       +  +  A KEWGFF I NHG+PK+ F  ++ + +++LF 
Sbjct: 39  KLPVIDLSHLTSGEEVKRKRCVKQMVAAAKEWGFFQIVNHGIPKDVFEMMLLE-EKKLFD 98

Query: 69  FPDETKRKLKVGASSYTPRFVVSPSLESFKVLGPNFSASAIAHGFTD--SLFGHQATQFR 128
            P   K + +    S       +PS  S       +S S   H      S         R
Sbjct: 99  QPFSVKVRERFSDLSKNSYRWGNPSATS----PAQYSVSEAFHIILSEVSRISDDRNNLR 158

Query: 129 NLLDEYGSKMIEISKRVVQLLLKTLGDDIETKFYDSEFSNCYGYIRINRYSPQDLNDEEE 188
            +++ Y  ++  +++ + ++L K +  ++ ++++++ F     ++R+N+Y P       E
Sbjct: 159 TIVETYVQEIARVAQMICEILGKQV--NVSSEYFENIFELENSFLRLNKYHPSVFG--SE 218

Query: 189 MDAFKQHTDISYVTILFQDDVGGLQMRSKGGEWFDVKPSEDGDLLVNIGDFMEGWSNGRV 248
           +     HTD S++TIL QD +GGL++ +  G+W  VKP  +  L VNIGD  +  SNG  
Sbjct: 219 VFGLVPHTDTSFLTILSQDQIGGLELEN-NGQWISVKPCLEA-LTVNIGDMFQALSNGVY 278

Query: 249 KSAEHRVVLRKNNVKTRFSVAFFLTNEDDDNKEIYAPSEVVGEGKSRLYLPFSTKEYR 300
           +S  HRV+   N    R S+AFF+          Y  +E+   G  + Y  FS +EY+
Sbjct: 279 QSVRHRVISPAN--IERMSIAFFVCP--------YLETEIDCFGYPKKYRRFSFREYK 315

[Close]
BLAST of ClCG09G007850 vs. TAIR 10
Match: AT4G21200.1 (gibberellin 2-oxidase 8 )

HSP 1 Score: 118.6 bits (296), Expect = 9.0e-27
Identity = 83/263 (31.56%), Postives = 129/263 (49.05%), Query Frame = 0 

Query: 9   EIPVMDID-----AHSFSESAICSLRRACKEWGFFFIRNHGVPKEFFRKLISDADRRLFT 68
           E+PV+D+      A    E    ++ RA +EWGFF + NHG+  +   K+  +   R+F 
Sbjct: 41  ELPVIDVSRLIDGAEEEREKCKEAIARASREWGFFQVINHGISMDVLEKMRQE-QIRVFR 100

Query: 69  FP-DETKRKLKVGASSYTPRFVVSPSLESFKVLGPNFSASAIAHGFTDSLFGHQATQFRN 128
            P D+  +  K  A SY      +PS  S + L     + A     TD       T   +
Sbjct: 101 EPFDKKSKSEKFSAGSYR---WGTPSATSIRQLS---WSEAFHVPMTDISDNKDFTTLSS 160

Query: 129 LLDEYGSKMIEISKRVVQLLLKTLGDDIETKFYDSEFSNCYGYIRINRYSPQDLNDEEEM 188
            ++++ S+   ++  + ++L +  G +  + F+         Y+R+NRY P       E+
Sbjct: 161 TMEKFASESEALAYMLAEVLAEKSGQN--SSFFKENCVRNTCYLRMNRYPP--CPKPSEV 220

Query: 189 DAFKQHTDISYVTILFQDDVGGLQMRSKGGEWFDVKPSEDGDLLVNIGDFMEGWSNGRVK 248
                HTD  ++TIL+QD VGGLQ+  K   W  VKP+    L++NIGD  + WSNG  K
Sbjct: 221 YGLMPHTDSDFLTILYQDQVGGLQL-IKDNRWIAVKPNPKA-LIINIGDLFQAWSNGMYK 280

Query: 249 SAEHRVVLRKNNVKTRFSVAFFL 266
           S EHRV+   N    RFS A+F+
Sbjct: 281 SVEHRVM--TNPKVERFSTAYFM 288

The following BLAST results are available for this feature:
BLAST of ClCG09G007850 vs. NCBI nr
Match NameE-valueIdentityDescription
XP_038899419.11.7e-15283.13gibberellin 20-oxidase-like protein [Benincasa hispida][more]
XP_022991453.11.0e-12070.74gibberellin 20-oxidase-like protein isoform X1 [Cucurbita maxima][more]
XP_022953360.15.0e-12067.41gibberellin 20-oxidase-like protein [Cucurbita moschata][more]
KAG6576054.16.5e-12067.41Gibberellin 20-oxidase-like protein, partial [Cucurbita argyrosperma subsp. soro... [more]
XP_022953356.11.4e-11969.50gibberellin 20-oxidase-like protein isoform X1 [Cucurbita moschata][more]
BLAST of ClCG09G007850 vs. ExPASy Swiss-Prot
Match NameE-valueIdentityDescription
F4KBY01.0e-6743.81Gibberellin 20-oxidase-like protein OS=Arabidopsis thaliana OX=3702 GN=At5g51310... [more]
Q9C6I41.5e-2628.19Gibberellin 2-beta-dioxygenase 7 OS=Arabidopsis thaliana OX=3702 GN=GA2OX7 PE=1 ... [more]
O495611.3e-2531.56Gibberellin 2-beta-dioxygenase 8 OS=Arabidopsis thaliana OX=3702 GN=GA2OX8 PE=1 ... [more]
Q9C8996.3e-2533.86Feruloyl CoA ortho-hydroxylase 2 OS=Arabidopsis thaliana OX=3702 GN=F6'H2 PE=1 S... [more]
P109678.2e-2529.331-aminocyclopropane-1-carboxylate oxidase homolog OS=Solanum lycopersicum OX=408... [more]
BLAST of ClCG09G007850 vs. ExPASy TrEMBL
Match NameE-valueIdentityDescription
A0A6J1JW964.9e-12170.74gibberellin 20-oxidase-like protein isoform X1 OS=Cucurbita maxima OX=3661 GN=LO... [more]
A0A6J1GPF22.4e-12067.41gibberellin 20-oxidase-like protein OS=Cucurbita moschata OX=3662 GN=LOC11145593... [more]
A0A6J1GMS47.0e-12069.50gibberellin 20-oxidase-like protein isoform X1 OS=Cucurbita moschata OX=3662 GN=... [more]
A0A6J1JSZ72.3e-11867.41gibberellin 20-oxidase-like protein OS=Cucurbita maxima OX=3661 GN=LOC111488065 ... [more]
A0A6J1GMT11.4e-11565.18gibberellin 20-oxidase-like protein OS=Cucurbita moschata OX=3662 GN=LOC11145593... [more]
BLAST of ClCG09G007850 vs. TAIR 10
Match NameE-valueIdentityDescription
AT4G23340.15.0e-7848.902-oxoglutarate (2OG) and Fe(II)-dependent oxygenase superfamily protein [more]
AT5G51310.17.3e-6943.812-oxoglutarate (2OG) and Fe(II)-dependent oxygenase superfamily protein [more]
AT4G23340.22.8e-5251.232-oxoglutarate (2OG) and Fe(II)-dependent oxygenase superfamily protein [more]
AT1G50960.11.1e-2728.19gibberellin 2-oxidase 7 [more]
AT4G21200.19.0e-2731.56gibberellin 2-oxidase 8 [more]
InterPro
Analysis Name: InterPro Annotations of Watermelon (Charleston Gray) v2.5
Date Performed: 2022-01-31
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR044861Isopenicillin N synthase-like, Fe(2+) 2OG dioxygenase domainPFAMPF031712OG-FeII_Oxycoord: 167..266
e-value: 8.9E-21
score: 74.3
IPR026992Non-haem dioxygenase N-terminal domainPFAMPF14226DIOX_Ncoord: 10..73
e-value: 1.1E-12
score: 48.8
IPR027443Isopenicillin N synthase-like superfamilyGENE3D2.60.120.330coord: 1..306
e-value: 2.0E-61
score: 210.1
NoneNo IPR availablePANTHERPTHR479902-OXOGLUTARATE (2OG) AND FE(II)-DEPENDENT OXYGENASE SUPERFAMILY PROTEIN-RELATEDcoord: 7..308
NoneNo IPR availablePANTHERPTHR47990:SF9GIBBERELLIN 20-OXIDASE-LIKE PROTEINcoord: 7..308
NoneNo IPR availableSUPERFAMILY51197Clavaminate synthase-likecoord: 8..306
IPR005123Oxoglutarate/iron-dependent dioxygenasePROSITEPS51471FE2OG_OXYcoord: 162..267
score: 12.245684

Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
ClCG09G007850.1ClCG09G007850.1mRNA


GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
molecular_function GO:0046872 metal ion binding
molecular_function GO:0016491 oxidoreductase activity