ClCG09G001620 (gene) Watermelon (Charleston Gray) v2.5

Overview
NameClCG09G001620
Typegene
OrganismCitrullus lanatus subsp. vulgaris cv. Charleston Gray (Watermelon (Charleston Gray) v2.5)
Description7-dehydrocholesterol reductase
LocationCG_Chr09: 1518429 .. 1524991 (-)
RNA-Seq ExpressionClCG09G001620
SyntenyClCG09G001620
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: exonfive_prime_UTRpolypeptideCDSthree_prime_UTR
Hold the cursor over a type above to highlight its positions in the sequence below.
CTCGTCTTCAAATCATCTACCCGGCGAGCTTGCTGTTGTGAGTACGGTTTCGCTAAAACTCAAACGCCTCAAAAATGGCGGAAGGATCCAACACCGTACACTCACCCTTCCTCACTTATGCTTCCATGTTATCTCTTCTCACTCTCTGCCCTCCCTTTGTTATCTTACTGTAAATCTTTTATCTCTCTCTCTGCTTCTTTATTGGAACAAATCTCCTTCAACTGTTCTTTACTGCGCCTTCCATCTTGGATCTCATCCATTTCTCTATATTCTACCTCTACGATTCAATATTCAACTAATTTCAACGATTCATCACAAATCTTGATCTGCTTGAATATCTCAACGTTTTTTTTCCTGAAATGCAGATGGTACACAATGGTCCATGCTGATGGGTCAGTTTCTCAGACTTGGAATTACTTATACCAGAATGGATTCAAGGGCTTTATAAATATGTGGCCACCGCCCTCTGCTACTGCTTGCAAGATAATTGGATGTTATGCTGCCTTTGAGGCTGCCCTTCAGCTTCTTTTACCTGGAAAGAGGGTTCTAGGCCCGGTCTCTCCAACAGGAAATCAACCTGTCTACAAGGTATTTGCACATTACACTCTCGATCATTTTGTTTCAGTTCTCTGCATTTCAAAGTCCAGTTAAAGTTACTAATGCCTCGACATTCATGATGTTGTCCATAGATCTGTTAATAATTTATTGCTCCTGTTCTGTGCTGCAGGCAAATGGCGTGGCTGCCTACATAGTGACACTGGTTACTTATACTGCTCTTTGGTGGTATGGTGTTGAGTCGATTTTCTTTCGTCTTTGGCCAAATGTTTGAGATTAAACAACCATGATGGATTTCTACTAACTTATCGCCCCTTCAGGAATTTATTGCAGTCTGTACCTTCAGTTTAGACAATTTACACTTTCGTAATTCATTTGCTGTACCAATTTTTTGTACATCTGTTCGTTTCGTCTATATATCTCAATCTTGGTCTATTGTTATCATTATATTTCTCCGACAGAGTCAGTATAGTGTTTATGCTAAAATTATATGTTTATACATATGTAGGTAATATCTTTTCTGCTTTCTCGATTTATTATGATATTTTTCTTGTTTCATTGTTTAATTGAAACTTTTATTCTTCAGGTTTGGGATATTTGATCCAACAATTGTTTATGATCATCTGGGAGAAATATTTTCGGCTCTTATTTTTGGAAGCTTCATCTTTTGTATTCTTCTATACATAAAAGTGAGGATTTAGATTTTTGTAACGATACCAGTTGAAGTTGAGTATATGCATATATTTGCTCTCTGTAAACGTAACACTGGTGTTTGCATGTACAAGGGTCATGTGGCGCCATCTTCCTCTGATTCTGGTTCCTCTGGTAACATAATACTTGATTTCTACTGGGTAAGCTTTCACATCCCTTTGATATTCATGACTTGACATGACAACTTTAATTTTTCTTTAGTTAAAGAAAAAAAAAACCCAGTTATTTTAAAGATAATGAAATTCAGAGAAGGCCACGCATAAAAACGGACTCAACTAACATGGGATAACAAAAAGGAAAAGAGAAATTACAAAATTATGAAAAAGGAACCTCGGCTGTTGGTTGTAAGAAAAATAGGCTGCCTGAAATTTTTTGAAGAGCAAACCTAATTAGAAGCATTAAAATGTTCGAGCCAGCAAACCCTTCTTACAACCTATGAACAGGTCGAACAACTAGCTCCTCGAAGTAAACAAATTTATCTCAAAATGGAGGACTCAATGCAACTTCCTCAATCATCGTGTGTGAACTAGAATTAGGACCATGAAAATTCCAAGGTCTACCAAAAACAACACCGCGCTTTGAAACAATGTGAGACTAATGAATATTATAGATGGTTGTTGAACTAAGTTACTTGTGAATGAACTACTGTATTCTACATATTCAGTTATTAGGGTAATCTGGCCCAAATCAGATGATAATCTCCGAGACATTCAAGTAGTTTGCATCTGCACTTATTGTCCTGAAAAGTTCCCTTGTTTTCTTACGTTATGATGCTACTATATATCAAATATATAAACATAGCTATAATCTTTTGCTTTATTATGTGTTCTGTGCTCGTTACTTTACTAATCAGCCTGCCTTTAATTTTTGGTCTGTTAATATATATGGTATGAGAACAAAATGGTGGGAACCCGACTGGCTTGGCATAAACTATGTTTCCTTTTGTTATAACTTCAATTTTTTTTATTTAACTCTGGATGATTCCTTGGAGCGAACAATATCTTTTTGCGGTGTATTTCGACTTCAGCTGCTATGTAATCTCCGCTAGAATTTCATTTAAATGCTTTTTGCTGCCTTGTGAAGTTATATATTTATGCAGTTTATGGACTTCCAATTTGGGAATTGAGAAAAATAGTATACTCTATACTTCAAGAGTTCATACAACTTGTCTATGTTTTGCTCACATGTTTTTTTCTTTTTTTTTTTTTTAGTTTTTTATTTGCTTAATTTTCTCTTTAGTTCTATTTAAAGCACCTGTAAAGAGAAAATTGAATAAGTACTAGAGTATTGTATTTTGTTTGATCACGTGATTGCATTTGCCAGTCTAGGTCTTGTTGATATTCTATAACACCTTTTTTTATTTACTTGCTTACAGGGCATGGAATTGTACCCGCGTATTGGCAAACATTTTGATATTAAGGTTTTCACAAACTGCAGATTTGGGATGATGTCTTGGGCCGTCCTTGCTGTGACCTATTGCATAAAGCAAGTAGGTAATCTAAAAGATCCCAAGTATGAAAGATAATCCTACATTTGTGGATGTTTTCTCTTCATATGAGGTTTACTAAATATTATTTATAATAATGGGATTCAAGTCTCGTTTAACTGATTGGCACCTTTGCTTTATCTATTATTGGTATTTTCTTCTGTAACATTTTTAAGGCAACAGAAGTTAAAATATGTGGTTCTTTTTTTTTTTTTTTTTTTTCATTTTAAATTTTGTTATTTTAAATTTCTATCTTATGAGTCAAGCCTCTTATTGCCAATAAATTTGTTTCTTTGCTTCTATCAAGGCCACATTAGTTTGTAGTAATCACACTTTTAAAGTAGAGTAGTTGGTAAACATTGATGATTGGAACTTTGGATGTGCGTTTTTGCACAAAGGAGCTTTGGAAGCTGGAGGCATATACTGTCTTACTGAAAGTACTTAGCTCTTATTTAGTAGTTTTTGGGGAGTGGGGAGTCTGTAGATAGTATGCTACTTTCTCTTGATACATATGATTTGATAGAGCCTTGTGATGTGATCAAGGAGTTCCTCCTCCATCCGTTTTTTTTTTCGAGAAGGATTGGGTTTTATGGCTTGCTGGGGTGTATGCTATATTGTGGGATCTTTCGGGTAAGCCAAATAACCGAGAATTTAAAGGGCTGGAAAGGGATCCTAGTGGTATTTGGTCCCTCATTAGATTTCGTATTTCCCTTTGGACGTCATGCTTCAATTTCAAAGACCTTTTGTAATTGCTCTATAGGCAATTTTCTGTAGATAGAGCCCCTTCTTCTAGTTGGGATCCTTTTTTGTGGGTTTGGTTTTCTGTATGCCCTTGTATTCTTTCAATTTTTTTCTCACTACCAACTTGGGGAAATGATGTACAACTCTGATGACCTAAGGGAAAAAGAAATTAAATTCACAGATGTTTGAGAGGCTTGCCTCTCCCAAGGCCAGAAACTATTTCCAGCTTTCACTTGCTGACAGACAGACTATTTCCTTCGCCCTCTCTTGTACATATAACCCAGACCATTTCCCACTCAAAATGACTGACTAACTAATTTTATGTCCTTCCGATGACTTTGCCAATAGTAGCAAACTATGATAAAGTTGTTGGATCTTGGCAAAATATTAGAAGAGCAGTTATCCCACATTGGAAAGATTTAAGAAAGTGATGAGCTGCAAACACTACAAAAGGAGTTTATGTCTTTGGTTTCAGATCATCTCAATTTGAAGCACTTTAAGCCTGGCATACTAACTCAATTGTGTGAGTGGTATAATGTTTTGAGAGAGTATTTTCTTTGTAATTAGAGTTGGAGAGAGATTTTTGTGTGATTGTAATAATTTTTCACATAGTGGAATTTTTTCTAGTCCGTGGTTTTTTTATCTAGTTTAGAGTTTTTCCACATTAATTCTTGTGTTTCTAGTTTCATTGCTTTCTCTTAATTTCTCAATTATTTTTCTATTAGTGGAAGGTTTTTTCCAACAAAGGTCTCACCCTACTTTGAACAACTACTTTGCCTTTGACCCACAGCACCCCATTTTCCCTCCTTAGATTCACTCTTTTCCCCCTTAACATAAACAATGTAGACTGCTTTAAATTCCTGTCAGAACATTTTATTTGTAGCAATCCTGAATCGTTAGAGGATCATCGTTTATTAACTGAGAAGTTGTATATTTTACCCAAAAGTTAGCTTTTACCCTTCTTTTTCCTGCTCTGCAAGCACTTATATGTATATTGTAGTTCTGTACCTATGTTGGTTCTATGGTAGTCAAAGTGTTGAGTGATGTAAATTGTTGCATAATTATCTTCTGTCTATTTCAGTTTGAAAAGAACGGGACAATAGCTGATTCAATGCTTGTAAATACTGCATTGATGTTGGTTTATGTTACAAAGTTTTTTTGGTGGGAAGCAGGGTACTGGAACACAATGGACATAGCGCATGATAGAGGTTAGCACATATTGTACGGTTTTCTTTGTTTAACATATTATTTCTTCATACCCTTCTGTTATTAATTAATGGTTAGATCAAACAAACGCCCTTGAACTTTAGTGCAAGTTTCAATTATGTCTCTAAACTTTGAAAAGTTCAACCTTTTCAAAATCTAGGAACATAATTGAAACCTAATCTGAAGTTCAGGATTATTTTGTATAATTTAGCCTAATTAAAAATGTTGGAGCACAATTGACTAGTTTTTTTTATTTTGCAACTCATTTGCATTTGAAATGTACTAGGATCTGATTTTAATTGTCCGTTTACCTTGTTGGAAATATAAATAAATAAATATATGTTATTCCTTTTGTTTTATCAATCTCAATATTCTTTGAAATTGGGAACTTGGATTTTTCAGCTGGTTTCTATATTTGTTGGGGATGCTTGGTATGGGTTCCGTCTGTATATACCTCTCCAGGCATGTATTTGGTCAACCATCCTGTAAACCTTGGAACTCAGGTATTCTTTCTTTCTGGTTAACTTCGAACATCAAGCAATTAATTTGAATTGAAACATGCTGCGTAATCACATTTTTTAGAGATTCGTGAAAACTCATTAGCTTTTGGATGGATGCTGGTTATCCAATGTGGAAAATACCCACTTTTTAAGAAACGCCATCTTAATATATTTATAGGCGTTTGGATATCAAATAAGAAAGATGATTTGAATAGCTAGATGCTTCATCGGGTGTGGTTGCATAGTTGTTTCTTAATCCAAAAACTCAGGACTCACCCAATCAGACGAGCTTGAAAGCAGATAGAAAAAATATATCTCTATTTGTATACATCTTTATGGACCGTGCTACTTTCCTAACAATCCCTTTTGGCTTTCAGTTGGCAGTATATATTCTAGTAGCAGGCATTCTTTGCGTATATATCAACTACGAATGTGACAGGCAGAGGCAAGAATTTCGCAGAAAAAGTGGGAAGTGCTTGGTTTGGGGAAAAGCTCCATCAAAGGTTTTGATGAGAATTCTATGTCCACAAGAATTCATATAGAACTAACTTGTGATATGATGTGATAAAATCTAGTATTAATTTGACAGATAGTCGCTTCATATACCACTACATCTGGGGATACCAAAACTAGTCTTCTTTTAACCTCTGGATGGTGAGTTTTCATTTGCTTCATTTCCTACATATACATAAGTGGGTGGGAAAGTATTGGACATTTTGCTGTTGGTGTTGATGCAGGTGGGGTTTAGCAAGGCATTTTCATTATGTCCCAGAAATTTTGGCTGCATTTTTCTGGACAGTTCCAGCCCTTTTTAATCATGTAAGCACCATCTATTCTAATCAATACAACAAACACCTAAGTTTCACCAGAAGGAAGAATTATATCATCTGTTTATGGTAATTGTCTTGTTGGTTCGAATCTCCTCTTCTGCAGTTTCTCCCATATTTCTATGTGATATTTCTTACTATCCTTCTGTTCGACCGAGCTAAAAGGGATGATGATCGATGTCGGTCCAAGTAAGTAAATCAGTTTGGTTCAGTTAATATGGGATGAGAAGTACTTTGGTTGATGATTTTGAAATCATGCAGGTATGGGAAATATTGGAAACTCTACTGCGAGAAAGTGCCTTACAAAATCATTCCTGGAATATACTAATTGTAGTTCCTACTTTGTGTATTACTTATTACAATCAGAATACCTTCTGTACACTATTACTTACGATGTTTCGGTTCACTTCTTGTAAACTCATGTCGCTCCCCAAAGTTGGGATTAGATTTGAACTCATCTTCTTAAAATTAAGTTATTTAAAGAAATTACTACCTTACTTATTTATAACACTTCCTTCATCCTTCTTTCCGTTGGTC

mRNA sequence

CTCGTCTTCAAATCATCTACCCGGCGAGCTTGCTGTTGTGAGTACGGTTTCGCTAAAACTCAAACGCCTCAAAAATGGCGGAAGGATCCAACACCGTACACTCACCCTTCCTCACTTATGCTTCCATGTTATCTCTTCTCACTCTCTGCCCTCCCTTTGTTATCTTACTATGGTACACAATGGTCCATGCTGATGGGTCAGTTTCTCAGACTTGGAATTACTTATACCAGAATGGATTCAAGGGCTTTATAAATATGTGGCCACCGCCCTCTGCTACTGCTTGCAAGATAATTGGATGTTATGCTGCCTTTGAGGCTGCCCTTCAGCTTCTTTTACCTGGAAAGAGGGTTCTAGGCCCGGTCTCTCCAACAGGAAATCAACCTGTCTACAAGGCAAATGGCGTGGCTGCCTACATAGTGACACTGGTTACTTATACTGCTCTTTGGTGGTTTGGGATATTTGATCCAACAATTGTTTATGATCATCTGGGAGAAATATTTTCGGCTCTTATTTTTGGAAGCTTCATCTTTTGTATTCTTCTATACATAAAAGGTCATGTGGCGCCATCTTCCTCTGATTCTGGTTCCTCTGGTAACATAATACTTGATTTCTACTGGGGCATGGAATTGTACCCGCGTATTGGCAAACATTTTGATATTAAGGTTTTCACAAACTGCAGATTTGGGATGATGTCTTGGGCCGTCCTTGCTGTGACCTATTGCATAAAGCAATTTGAAAAGAACGGGACAATAGCTGATTCAATGCTTGTAAATACTGCATTGATGTTGGTTTATGTTACAAAGTTTTTTTGGTGGGAAGCAGGGTACTGGAACACAATGGACATAGCGCATGATAGAGCTGGTTTCTATATTTGTTGGGGATGCTTGGTATGGGTTCCGTCTGTATATACCTCTCCAGGCATGTATTTGGTCAACCATCCTGTAAACCTTGGAACTCAGTTGGCAGTATATATTCTAGTAGCAGGCATTCTTTGCGTATATATCAACTACGAATGTGACAGGCAGAGGCAAGAATTTCGCAGAAAAAGTGGGAAGTGCTTGGTTTGGGGAAAAGCTCCATCAAAGATAGTCGCTTCATATACCACTACATCTGGGGATACCAAAACTAGTCTTCTTTTAACCTCTGGATGGTGGGGTTTAGCAAGGCATTTTCATTATGTCCCAGAAATTTTGGCTGCATTTTTCTGGACAGTTCCAGCCCTTTTTAATCATTTTCTCCCATATTTCTATGTGATATTTCTTACTATCCTTCTGTTCGACCGAGCTAAAAGGGATGATGATCGATGTCGGTCCAAGTATGGGAAATATTGGAAACTCTACTGCGAGAAAGTGCCTTACAAAATCATTCCTGGAATATACTAATTGTAGTTCCTACTTTGTGTATTACTTATTACAATCAGAATACCTTCTGTACACTATTACTTACGATGTTTCGGTTCACTTCTTGTAAACTCATGTCGCTCCCCAAAGTTGGGATTAGATTTGAACTCATCTTCTTAAAATTAAGTTATTTAAAGAAATTACTACCTTACTTATTTATAACACTTCCTTCATCCTTCTTTCCGTTGGTC

Coding sequence (CDS)

ATGGCGGAAGGATCCAACACCGTACACTCACCCTTCCTCACTTATGCTTCCATGTTATCTCTTCTCACTCTCTGCCCTCCCTTTGTTATCTTACTATGGTACACAATGGTCCATGCTGATGGGTCAGTTTCTCAGACTTGGAATTACTTATACCAGAATGGATTCAAGGGCTTTATAAATATGTGGCCACCGCCCTCTGCTACTGCTTGCAAGATAATTGGATGTTATGCTGCCTTTGAGGCTGCCCTTCAGCTTCTTTTACCTGGAAAGAGGGTTCTAGGCCCGGTCTCTCCAACAGGAAATCAACCTGTCTACAAGGCAAATGGCGTGGCTGCCTACATAGTGACACTGGTTACTTATACTGCTCTTTGGTGGTTTGGGATATTTGATCCAACAATTGTTTATGATCATCTGGGAGAAATATTTTCGGCTCTTATTTTTGGAAGCTTCATCTTTTGTATTCTTCTATACATAAAAGGTCATGTGGCGCCATCTTCCTCTGATTCTGGTTCCTCTGGTAACATAATACTTGATTTCTACTGGGGCATGGAATTGTACCCGCGTATTGGCAAACATTTTGATATTAAGGTTTTCACAAACTGCAGATTTGGGATGATGTCTTGGGCCGTCCTTGCTGTGACCTATTGCATAAAGCAATTTGAAAAGAACGGGACAATAGCTGATTCAATGCTTGTAAATACTGCATTGATGTTGGTTTATGTTACAAAGTTTTTTTGGTGGGAAGCAGGGTACTGGAACACAATGGACATAGCGCATGATAGAGCTGGTTTCTATATTTGTTGGGGATGCTTGGTATGGGTTCCGTCTGTATATACCTCTCCAGGCATGTATTTGGTCAACCATCCTGTAAACCTTGGAACTCAGTTGGCAGTATATATTCTAGTAGCAGGCATTCTTTGCGTATATATCAACTACGAATGTGACAGGCAGAGGCAAGAATTTCGCAGAAAAAGTGGGAAGTGCTTGGTTTGGGGAAAAGCTCCATCAAAGATAGTCGCTTCATATACCACTACATCTGGGGATACCAAAACTAGTCTTCTTTTAACCTCTGGATGGTGGGGTTTAGCAAGGCATTTTCATTATGTCCCAGAAATTTTGGCTGCATTTTTCTGGACAGTTCCAGCCCTTTTTAATCATTTTCTCCCATATTTCTATGTGATATTTCTTACTATCCTTCTGTTCGACCGAGCTAAAAGGGATGATGATCGATGTCGGTCCAAGTATGGGAAATATTGGAAACTCTACTGCGAGAAAGTGCCTTACAAAATCATTCCTGGAATATACTAA

Protein sequence

MAEGSNTVHSPFLTYASMLSLLTLCPPFVILLWYTMVHADGSVSQTWNYLYQNGFKGFINMWPPPSATACKIIGCYAAFEAALQLLLPGKRVLGPVSPTGNQPVYKANGVAAYIVTLVTYTALWWFGIFDPTIVYDHLGEIFSALIFGSFIFCILLYIKGHVAPSSSDSGSSGNIILDFYWGMELYPRIGKHFDIKVFTNCRFGMMSWAVLAVTYCIKQFEKNGTIADSMLVNTALMLVYVTKFFWWEAGYWNTMDIAHDRAGFYICWGCLVWVPSVYTSPGMYLVNHPVNLGTQLAVYILVAGILCVYINYECDRQRQEFRRKSGKCLVWGKAPSKIVASYTTTSGDTKTSLLLTSGWWGLARHFHYVPEILAAFFWTVPALFNHFLPYFYVIFLTILLFDRAKRDDDRCRSKYGKYWKLYCEKVPYKIIPGIY
Homology
BLAST of ClCG09G001620 vs. NCBI nr
Match: XP_011659586.1 (7-dehydrocholesterol reductase isoform X1 [Cucumis sativus] >KGN45416.1 hypothetical protein Csa_016668 [Cucumis sativus])

HSP 1 Score: 887.1 bits (2291), Expect = 6.1e-254
Identity = 412/435 (94.71%), Postives = 428/435 (98.39%), Query Frame = 0

Query: 1   MAEGSNTVHSPFLTYASMLSLLTLCPPFVILLWYTMVHADGSVSQTWNYLYQNGFKGFIN 60
           MAEGSNTVHSPFLTY SMLSLLTLCPPFVILLWYTMVHADGSVSQTWNYLYQNGF+GFIN
Sbjct: 1   MAEGSNTVHSPFLTYVSMLSLLTLCPPFVILLWYTMVHADGSVSQTWNYLYQNGFEGFIN 60

Query: 61  MWPPPSATACKIIGCYAAFEAALQLLLPGKRVLGPVSPTGNQPVYKANGVAAYIVTLVTY 120
           +WP PSATACKIIGCYAAFEAALQLLLPGKRVLGPVSPTGNQPVYKANGVAAYIVTL+TY
Sbjct: 61  IWPSPSATACKIIGCYAAFEAALQLLLPGKRVLGPVSPTGNQPVYKANGVAAYIVTLLTY 120

Query: 121 TALWWFGIFDPTIVYDHLGEIFSALIFGSFIFCILLYIKGHVAPSSSDSGSSGNIILDFY 180
            ALW FGIFDP IVYDHLGEI+S LIFGSFIFCILLYIKGHVAPSSSDSGSSGN+ILDFY
Sbjct: 121 VALWRFGIFDPAIVYDHLGEIYSTLIFGSFIFCILLYIKGHVAPSSSDSGSSGNVILDFY 180

Query: 181 WGMELYPRIGKHFDIKVFTNCRFGMMSWAVLAVTYCIKQFEKNGTIADSMLVNTALMLVY 240
           WGMELYPRIGKHFDIKVFTNCRFGMMSWAVLAVTYCIKQ+EKNGT+ADS+LVNTALMLVY
Sbjct: 181 WGMELYPRIGKHFDIKVFTNCRFGMMSWAVLAVTYCIKQYEKNGTLADSLLVNTALMLVY 240

Query: 241 VTKFFWWEAGYWNTMDIAHDRAGFYICWGCLVWVPSVYTSPGMYLVNHPVNLGTQLAVYI 300
           VTKFFWWEAGYWNTMDIAHDRAGFYICWGCLVWVPSVYTSPGMYLVNHPVNLGTQLAV I
Sbjct: 241 VTKFFWWEAGYWNTMDIAHDRAGFYICWGCLVWVPSVYTSPGMYLVNHPVNLGTQLAVSI 300

Query: 301 LVAGILCVYINYECDRQRQEFRRKSGKCLVWGKAPSKIVASYTTTSGDTKTSLLLTSGWW 360
           LVAGILC+YINY+CDRQRQEFRRK+GKCLVWGKAPSKIVA+YTTT+G+T+TSLLLTSGWW
Sbjct: 301 LVAGILCIYINYDCDRQRQEFRRKNGKCLVWGKAPSKIVATYTTTTGETRTSLLLTSGWW 360

Query: 361 GLARHFHYVPEILAAFFWTVPALFNHFLPYFYVIFLTILLFDRAKRDDDRCRSKYGKYWK 420
           GLARHFHYVPEILAAFFWTVPALF+HFLPYFYVIFLTILLFDRAKRDDDRCRSKYGKYWK
Sbjct: 361 GLARHFHYVPEILAAFFWTVPALFHHFLPYFYVIFLTILLFDRAKRDDDRCRSKYGKYWK 420

Query: 421 LYCEKVPYKIIPGIY 436
           LYCEKVPYKIIPGIY
Sbjct: 421 LYCEKVPYKIIPGIY 435

BLAST of ClCG09G001620 vs. NCBI nr
Match: XP_008462206.1 (PREDICTED: 7-dehydrocholesterol reductase [Cucumis melo] >KAA0059337.1 7-dehydrocholesterol reductase [Cucumis melo var. makuwa] >TYK03988.1 7-dehydrocholesterol reductase [Cucumis melo var. makuwa])

HSP 1 Score: 886.3 bits (2289), Expect = 1.0e-253
Identity = 416/435 (95.63%), Postives = 426/435 (97.93%), Query Frame = 0

Query: 1   MAEGSNTVHSPFLTYASMLSLLTLCPPFVILLWYTMVHADGSVSQTWNYLYQNGFKGFIN 60
           MAEGSNTVHSPFLTY SMLSLLTLCPPFVILLWYTMVHADGSVSQTWNYLYQNGF+GFIN
Sbjct: 1   MAEGSNTVHSPFLTYVSMLSLLTLCPPFVILLWYTMVHADGSVSQTWNYLYQNGFEGFIN 60

Query: 61  MWPPPSATACKIIGCYAAFEAALQLLLPGKRVLGPVSPTGNQPVYKANGVAAYIVTLVTY 120
           +WP PSATACKIIGCYAAFEAALQLLLPGKRVLGPVSPTGNQPVYKANGVAAYIVTLVTY
Sbjct: 61  IWPSPSATACKIIGCYAAFEAALQLLLPGKRVLGPVSPTGNQPVYKANGVAAYIVTLVTY 120

Query: 121 TALWWFGIFDPTIVYDHLGEIFSALIFGSFIFCILLYIKGHVAPSSSDSGSSGNIILDFY 180
            ALW FGIFDP IVYDHLGEIFS LIFGSFIFCILLYIKGHVAPSSSDSGSSGNIILDFY
Sbjct: 121 VALWRFGIFDPAIVYDHLGEIFSTLIFGSFIFCILLYIKGHVAPSSSDSGSSGNIILDFY 180

Query: 181 WGMELYPRIGKHFDIKVFTNCRFGMMSWAVLAVTYCIKQFEKNGTIADSMLVNTALMLVY 240
           WGMELYPRIGKHFDIKVFTNCRFGMMSWAVLAVTYCIKQ+EKNG +ADSMLVNTALMLVY
Sbjct: 181 WGMELYPRIGKHFDIKVFTNCRFGMMSWAVLAVTYCIKQYEKNGILADSMLVNTALMLVY 240

Query: 241 VTKFFWWEAGYWNTMDIAHDRAGFYICWGCLVWVPSVYTSPGMYLVNHPVNLGTQLAVYI 300
           VTKFFWWEAGYWNTMDIAHDRAGFYICWGCLVWVPSVYTSPGMYLVNHPVNLGTQLAV I
Sbjct: 241 VTKFFWWEAGYWNTMDIAHDRAGFYICWGCLVWVPSVYTSPGMYLVNHPVNLGTQLAVSI 300

Query: 301 LVAGILCVYINYECDRQRQEFRRKSGKCLVWGKAPSKIVASYTTTSGDTKTSLLLTSGWW 360
           LVAGILC+YINY+CDRQRQEFRRK+GK LVWGKAPSKIVASYTTTSG+T+TSLLLTSGWW
Sbjct: 301 LVAGILCIYINYDCDRQRQEFRRKNGKRLVWGKAPSKIVASYTTTSGETRTSLLLTSGWW 360

Query: 361 GLARHFHYVPEILAAFFWTVPALFNHFLPYFYVIFLTILLFDRAKRDDDRCRSKYGKYWK 420
           GLARHFHYVPEILAAFFWTVPALF+HFLPYFYVIFLTILLFDRAKRDDDRCRSKYGKYWK
Sbjct: 361 GLARHFHYVPEILAAFFWTVPALFHHFLPYFYVIFLTILLFDRAKRDDDRCRSKYGKYWK 420

Query: 421 LYCEKVPYKIIPGIY 436
           LYCEKVPYKIIPGIY
Sbjct: 421 LYCEKVPYKIIPGIY 435

BLAST of ClCG09G001620 vs. NCBI nr
Match: XP_038898375.1 (7-dehydrocholesterol reductase isoform X1 [Benincasa hispida])

HSP 1 Score: 884.4 bits (2284), Expect = 4.0e-253
Identity = 416/435 (95.63%), Postives = 426/435 (97.93%), Query Frame = 0

Query: 1   MAEGSNTVHSPFLTYASMLSLLTLCPPFVILLWYTMVHADGSVSQTWNYLYQNGFKGFIN 60
           MAEGSNTVHSPFLTYASMLSLLTLCPPFVILLWYTMVHADGSVSQTW YLYQNGFKGFIN
Sbjct: 1   MAEGSNTVHSPFLTYASMLSLLTLCPPFVILLWYTMVHADGSVSQTWKYLYQNGFKGFIN 60

Query: 61  MWPPPSATACKIIGCYAAFEAALQLLLPGKRVLGPVSPTGNQPVYKANGVAAYIVTLVTY 120
           MWPPPSATACKIIGCYAAFEAALQLLLPGK+VLGPVSPTGNQPVYKANGVAAYIVTLVTY
Sbjct: 61  MWPPPSATACKIIGCYAAFEAALQLLLPGKKVLGPVSPTGNQPVYKANGVAAYIVTLVTY 120

Query: 121 TALWWFGIFDPTIVYDHLGEIFSALIFGSFIFCILLYIKGHVAPSSSDSGSSGNIILDFY 180
            ALW F IFDPTIVYDHLGEIFSALIFGSFIFCILLYIKGHVAPSSSDSGSSGNIILDFY
Sbjct: 121 IALWRFRIFDPTIVYDHLGEIFSALIFGSFIFCILLYIKGHVAPSSSDSGSSGNIILDFY 180

Query: 181 WGMELYPRIGKHFDIKVFTNCRFGMMSWAVLAVTYCIKQFEKNGTIADSMLVNTALMLVY 240
           WGMELYPRIGKHFDIKVFTNCRFGMMSWAVLAVTYCIKQ+EK GTIADSMLV+TALMLVY
Sbjct: 181 WGMELYPRIGKHFDIKVFTNCRFGMMSWAVLAVTYCIKQYEKYGTIADSMLVSTALMLVY 240

Query: 241 VTKFFWWEAGYWNTMDIAHDRAGFYICWGCLVWVPSVYTSPGMYLVNHPVNLGTQLAVYI 300
           VTKFFWWEAGYWNTMDIAHDRAGFYICWGCLVWVPSVY SPG+YLVNHPV +GTQLAV I
Sbjct: 241 VTKFFWWEAGYWNTMDIAHDRAGFYICWGCLVWVPSVYISPGLYLVNHPVKIGTQLAVSI 300

Query: 301 LVAGILCVYINYECDRQRQEFRRKSGKCLVWGKAPSKIVASYTTTSGDTKTSLLLTSGWW 360
           LVAGILCVYINY+CD QRQEFRRKSGKCLVWGKAPSKIVASYTTTSG+T+TSLLLTSGWW
Sbjct: 301 LVAGILCVYINYDCDGQRQEFRRKSGKCLVWGKAPSKIVASYTTTSGETRTSLLLTSGWW 360

Query: 361 GLARHFHYVPEILAAFFWTVPALFNHFLPYFYVIFLTILLFDRAKRDDDRCRSKYGKYWK 420
           GLARHFHYVPEILAAFFWTVPALF+HFLPYFYVIFLTILLFDRAKRDDDRCRSKYGKYWK
Sbjct: 361 GLARHFHYVPEILAAFFWTVPALFHHFLPYFYVIFLTILLFDRAKRDDDRCRSKYGKYWK 420

Query: 421 LYCEKVPYKIIPGIY 436
           LYCEKVPY+IIPGIY
Sbjct: 421 LYCEKVPYRIIPGIY 435

BLAST of ClCG09G001620 vs. NCBI nr
Match: XP_022147941.1 (7-dehydrocholesterol reductase [Momordica charantia])

HSP 1 Score: 870.5 bits (2248), Expect = 5.9e-249
Identity = 403/435 (92.64%), Postives = 419/435 (96.32%), Query Frame = 0

Query: 1   MAEGSNTVHSPFLTYASMLSLLTLCPPFVILLWYTMVHADGSVSQTWNYLYQNGFKGFIN 60
           MAEGS TVHSP LTY SMLSLL +CPPFVI LWYTMVHADGSVSQTWNYLY NG +GF+N
Sbjct: 1   MAEGSTTVHSPILTYVSMLSLLIICPPFVIFLWYTMVHADGSVSQTWNYLYHNGVRGFMN 60

Query: 61  MWPPPSATACKIIGCYAAFEAALQLLLPGKRVLGPVSPTGNQPVYKANGVAAYIVTLVTY 120
           +WPPPSATACKIIGCYAAFEAALQLLLPG RV GPVSPTGNQPVYKANGVAAYIVTLVTY
Sbjct: 61  IWPPPSATACKIIGCYAAFEAALQLLLPGNRVQGPVSPTGNQPVYKANGVAAYIVTLVTY 120

Query: 121 TALWWFGIFDPTIVYDHLGEIFSALIFGSFIFCILLYIKGHVAPSSSDSGSSGNIILDFY 180
            ALWWFGIF+PTIVYDHLGEIFSALIFGS IFCI LYIKGHVAPSSSDSGSSGNII+DFY
Sbjct: 121 IALWWFGIFNPTIVYDHLGEIFSALIFGSLIFCIFLYIKGHVAPSSSDSGSSGNIIIDFY 180

Query: 181 WGMELYPRIGKHFDIKVFTNCRFGMMSWAVLAVTYCIKQFEKNGTIADSMLVNTALMLVY 240
           WGMELYPRIGK+FDIKVFTNCRFGMMSWAVLAVTYCIKQ+EKNGTIADSMLVNTALMLVY
Sbjct: 181 WGMELYPRIGKNFDIKVFTNCRFGMMSWAVLAVTYCIKQYEKNGTIADSMLVNTALMLVY 240

Query: 241 VTKFFWWEAGYWNTMDIAHDRAGFYICWGCLVWVPSVYTSPGMYLVNHPVNLGTQLAVYI 300
           VTKFFWWE+GYWNTMDIAHDRAGFYICWGCLVWVPSVYTSPGMYLVNHPVNLGTQLAVYI
Sbjct: 241 VTKFFWWESGYWNTMDIAHDRAGFYICWGCLVWVPSVYTSPGMYLVNHPVNLGTQLAVYI 300

Query: 301 LVAGILCVYINYECDRQRQEFRRKSGKCLVWGKAPSKIVASYTTTSGDTKTSLLLTSGWW 360
           LVAG+LC+YINY+CDRQRQEFRRK+GKCL+W KAPSKIVASYTTTSG+TKTSLLLTSGWW
Sbjct: 301 LVAGLLCIYINYDCDRQRQEFRRKNGKCLIWRKAPSKIVASYTTTSGETKTSLLLTSGWW 360

Query: 361 GLARHFHYVPEILAAFFWTVPALFNHFLPYFYVIFLTILLFDRAKRDDDRCRSKYGKYWK 420
           GLARHFHY PEILAAFFWTVPALFNHFLPYFYVIFLTILL DRAKRDDDRCRSKYGKYWK
Sbjct: 361 GLARHFHYAPEILAAFFWTVPALFNHFLPYFYVIFLTILLLDRAKRDDDRCRSKYGKYWK 420

Query: 421 LYCEKVPYKIIPGIY 436
           LYCEKV Y+IIPGIY
Sbjct: 421 LYCEKVAYRIIPGIY 435

BLAST of ClCG09G001620 vs. NCBI nr
Match: XP_022964233.1 (7-dehydrocholesterol reductase [Cucurbita moschata])

HSP 1 Score: 859.4 bits (2219), Expect = 1.4e-245
Identity = 400/434 (92.17%), Postives = 418/434 (96.31%), Query Frame = 0

Query: 1   MAEGSNTVHSPFLTYASMLSLLTLCPPFVILLWYTMVHADGSVSQTWNYLYQNGFKGFIN 60
           MAEGSNTVHSP +TYASMLSLLTLCPPFVI LWYTMVHADGSVSQT  YLY NG +G I+
Sbjct: 1   MAEGSNTVHSPLVTYASMLSLLTLCPPFVIFLWYTMVHADGSVSQTMAYLYHNGLRGLID 60

Query: 61  MWPPPSATACKIIGCYAAFEAALQLLLPGKRVLGPVSPTGNQPVYKANGVAAYIVTLVTY 120
           +WPPPSATA K+IG YAAFEAALQLLLPGK V GP+SPTGNQPVYKANGVAAY+VTLVTY
Sbjct: 61  IWPPPSATAFKLIGSYAAFEAALQLLLPGKTVYGPISPTGNQPVYKANGVAAYVVTLVTY 120

Query: 121 TALWWFGIFDPTIVYDHLGEIFSALIFGSFIFCILLYIKGHVAPSSSDSGSSGNIILDFY 180
            ALWWFGIF+PT+VYDHLGEIFSALI GSFIFCI+LYIKG VAPSSSDSGSSGNII+DFY
Sbjct: 121 IALWWFGIFNPTVVYDHLGEIFSALIIGSFIFCIVLYIKGCVAPSSSDSGSSGNIIIDFY 180

Query: 181 WGMELYPRIGKHFDIKVFTNCRFGMMSWAVLAVTYCIKQFEKNGTIADSMLVNTALMLVY 240
           WGMELYPRIGKHFDIKVFTNCRFGMMSWAVLAVTYCIKQ+EKNGTIADSMLVNTALML Y
Sbjct: 181 WGMELYPRIGKHFDIKVFTNCRFGMMSWAVLAVTYCIKQYEKNGTIADSMLVNTALMLAY 240

Query: 241 VTKFFWWEAGYWNTMDIAHDRAGFYICWGCLVWVPSVYTSPGMYLVNHPVNLGTQLAVYI 300
           VTKFFWWEAGYWNTMDIAHDRAGFYICWGCLVWVPSVYTSPGMYLVNHPVNLGTQLAVYI
Sbjct: 241 VTKFFWWEAGYWNTMDIAHDRAGFYICWGCLVWVPSVYTSPGMYLVNHPVNLGTQLAVYI 300

Query: 301 LVAGILCVYINYECDRQRQEFRRKSGKCLVWGKAPSKIVASYTTTSGDTKTSLLLTSGWW 360
           LVAG+LC+YINY+CDRQRQEFRRK+GKCLVWGKAPSKIVASYTTTSG+TKTSLLLTSGWW
Sbjct: 301 LVAGMLCIYINYDCDRQRQEFRRKNGKCLVWGKAPSKIVASYTTTSGETKTSLLLTSGWW 360

Query: 361 GLARHFHYVPEILAAFFWTVPALFNHFLPYFYVIFLTILLFDRAKRDDDRCRSKYGKYWK 420
           GLARHFHYVPEILAAFFWTVPALFNHFLPYFYVIFLTILL DRAKRDDDRCRSKYGKYWK
Sbjct: 361 GLARHFHYVPEILAAFFWTVPALFNHFLPYFYVIFLTILLVDRAKRDDDRCRSKYGKYWK 420

Query: 421 LYCEKVPYKIIPGI 435
           LYCEKVPY+IIPGI
Sbjct: 421 LYCEKVPYRIIPGI 434

BLAST of ClCG09G001620 vs. ExPASy Swiss-Prot
Match: Q9LDU6 (7-dehydrocholesterol reductase OS=Arabidopsis thaliana OX=3702 GN=DWF5 PE=1 SV=1)

HSP 1 Score: 802.4 bits (2071), Expect = 2.6e-231
Identity = 368/431 (85.38%), Postives = 399/431 (92.58%), Query Frame = 0

Query: 5   SNTVHSPFLTYASMLSLLTLCPPFVILLWYTMVHADGSVSQTWNYLYQNGFKGFINMWPP 64
           + TVHSP +TYASMLSLL  CPPFVILLWYTMVH DGSV+QT+ + ++NG +G IN+WP 
Sbjct: 2   AETVHSPIVTYASMLSLLAFCPPFVILLWYTMVHQDGSVTQTFGFFWENGVQGLINIWPR 61

Query: 65  PSATACKIIGCYAAFEAALQLLLPGKRVLGPVSPTGNQPVYKANGVAAYIVTLVTYTALW 124
           P+  A KII CY AFEA LQLLLPGKRV GP+SP GN+PVYKANG+AAY VTL TY  LW
Sbjct: 62  PTLIAWKIIFCYGAFEAILQLLLPGKRVEGPISPAGNRPVYKANGLAAYFVTLATYLGLW 121

Query: 125 WFGIFDPTIVYDHLGEIFSALIFGSFIFCILLYIKGHVAPSSSDSGSSGNIILDFYWGME 184
           WFGIF+P IVYDHLGEIFSALIFGSFIFC+LLYIKGHVAPSSSDSGS GN+I+DFYWGME
Sbjct: 122 WFGIFNPAIVYDHLGEIFSALIFGSFIFCVLLYIKGHVAPSSSDSGSCGNLIIDFYWGME 181

Query: 185 LYPRIGKHFDIKVFTNCRFGMMSWAVLAVTYCIKQFEKNGTIADSMLVNTALMLVYVTKF 244
           LYPRIGK FDIKVFTNCRFGMMSWAVLAVTYCIKQ+E NG ++DSMLVNT LMLVYVTKF
Sbjct: 182 LYPRIGKSFDIKVFTNCRFGMMSWAVLAVTYCIKQYEINGKVSDSMLVNTILMLVYVTKF 241

Query: 245 FWWEAGYWNTMDIAHDRAGFYICWGCLVWVPSVYTSPGMYLVNHPVNLGTQLAVYILVAG 304
           FWWEAGYWNTMDIAHDRAGFYICWGCLVWVPSVYTSPGMYLVNHPV LGTQLA+YILVAG
Sbjct: 242 FWWEAGYWNTMDIAHDRAGFYICWGCLVWVPSVYTSPGMYLVNHPVELGTQLAIYILVAG 301

Query: 305 ILCVYINYECDRQRQEFRRKSGKCLVWGKAPSKIVASYTTTSGDTKTSLLLTSGWWGLAR 364
           ILC+YINY+CDRQRQEFRR +GKCLVWG+APSKIVASYTTTSG+TKTSLLLTSGWWGLAR
Sbjct: 302 ILCIYINYDCDRQRQEFRRTNGKCLVWGRAPSKIVASYTTTSGETKTSLLLTSGWWGLAR 361

Query: 365 HFHYVPEILAAFFWTVPALFNHFLPYFYVIFLTILLFDRAKRDDDRCRSKYGKYWKLYCE 424
           HFHYVPEIL+AFFWTVPALF++FL YFYVIFLT+LLFDRAKRDDDRCRSKYGKYWKLYCE
Sbjct: 362 HFHYVPEILSAFFWTVPALFDNFLAYFYVIFLTLLLFDRAKRDDDRCRSKYGKYWKLYCE 421

Query: 425 KVPYKIIPGIY 436
           KV Y+IIPGIY
Sbjct: 422 KVKYRIIPGIY 432

BLAST of ClCG09G001620 vs. ExPASy Swiss-Prot
Match: O88455 (7-dehydrocholesterol reductase OS=Mus musculus OX=10090 GN=Dhcr7 PE=1 SV=1)

HSP 1 Score: 286.2 bits (731), Expect = 6.3e-76
Identity = 159/442 (35.97%), Postives = 249/442 (56.33%), Query Frame = 0

Query: 12  FLTYASMLSLLTLCPPFVILLWYTMVHADGSVSQTWNYL-YQNGFKGFINMW---PPPSA 71
           + + AS++ LL   P   I+ ++ M     S S T   L    G     ++W   PP +A
Sbjct: 33  WFSLASIIFLLLFAP--FIVYYFIMACDQYSCSLTAPALDIATGHASLADIWAKTPPVTA 92

Query: 72  TACKIIGCYAAFEAALQLLLP-----------GKRVLGPVSPTGNQPVYKANGVAAYIVT 131
            A ++   + +F+  L   LP           G    G ++P G    Y+ NG+ A+++T
Sbjct: 93  KAAQLYALWVSFQVLLYSWLPDFCHRFLPGYVGGVQEGAITPAGVVNKYEVNGLQAWLIT 152

Query: 132 -LVTYTALWWFGIFDPTIVYDH-LGEIFSALIFGSFIFCILLYIKGHVAPSSS-DSGSSG 191
            ++ +   +    F PTI++D+ +  ++ A I G +       IKG++ P+S+ D   +G
Sbjct: 153 HILWFVNAYLLSWFSPTIIFDNWIPLLWCANILG-YAVSTFAMIKGYLFPTSAEDCKFTG 212

Query: 192 NIILDFYWGMELYPRIGKHFDIKVFTNCRFGMMSWAVLAVTYCIKQFEKNGTIADSMLVN 251
           N   ++  G+E  PRIGK FD K+F N R G+++W ++ +++  KQ E  G + +SM++ 
Sbjct: 213 NFFYNYMMGIEFNPRIGKWFDFKLFFNGRPGIVAWTLINLSFAAKQQELYGHVTNSMILV 272

Query: 252 TALMLVYVTKFFWWEAGYWNTMDIAHDRAGFYICWGCLVWVPSVYTSPGMYLVNHPVNLG 311
             L  +YV  FFW E  Y  T+DI HD  G+Y+ WG  VW+P +YT  G+YLV HPV L 
Sbjct: 273 NVLQAIYVLDFFWNETWYLKTIDICHDHFGWYLGWGDCVWLPYLYTLQGLYLVYHPVQLS 332

Query: 312 TQLAVYILVAGILCVYINYECDRQRQEFRRKSGKCLVWGKAPSKIVASYTTTSGDTKTSL 371
           T  A+ IL+ G++  YI    + Q+  FRR  G+CL+WGK P  I  SYT+  G    S 
Sbjct: 333 TPNALGILLLGLVGYYIFRMTNHQKDLFRRTDGRCLIWGKKPKAIECSYTSADGLKHHSK 392

Query: 372 LLTSGWWGLARHFHYVPEILAAFFWTVPALFNHFLPYFYVIFLTILLFDRAKRDDDRCRS 431
           LL SG+WG+ARHF+Y  +++ +  + +     H LPYFY+I++TILL  R  RD+ RC +
Sbjct: 393 LLVSGFWGVARHFNYTGDLMGSLAYCLACGGGHLLPYFYIIYMTILLTHRCLRDEHRCAN 452

Query: 432 KYGKYWKLYCEKVPYKIIPGIY 436
           KYG+ W+ Y   VPY+++PGI+
Sbjct: 453 KYGRDWERYTAAVPYRLLPGIF 471

BLAST of ClCG09G001620 vs. ExPASy Swiss-Prot
Match: Q5E9J5 (7-dehydrocholesterol reductase OS=Bos taurus OX=9913 GN=DHCR7 PE=2 SV=1)

HSP 1 Score: 282.3 bits (721), Expect = 9.0e-75
Identity = 146/387 (37.73%), Postives = 225/387 (58.14%), Query Frame = 0

Query: 63  PPPSATACKIIGCYAAFEAALQLLLP-----------GKRVLGPVSPTGNQPVYKANGVA 122
           PP +A A +I   +   +  L +LLP           G    G V+P G    Y+ NG+ 
Sbjct: 90  PPVTAKAAQIYTAWVTLQVLLYMLLPDFCHKFLPGYVGGVQEGAVTPAGAVNKYEINGLQ 149

Query: 123 AYIVT-LVTYTALWWFGIFDPTIVYDH-LGEIFSALIFGSFIFCILLYIKGHVAPSSS-D 182
           A+++T L+ +      G F PTI++D+ +  ++ A I G +       +KG++ P+ + +
Sbjct: 150 AWLLTHLLWFANAHLLGWFSPTIIFDNWIPLLWCANILG-YTVSTFAMVKGYLFPTDARE 209

Query: 183 SGSSGNIILDFYWGMELYPRIGKHFDIKVFTNCRFGMMSWAVLAVTYCIKQFEKNGTIAD 242
              +GN   ++  G+E  PRIGK FD K+F N R G+++W ++ +++  KQ E  G + +
Sbjct: 210 CKFTGNFFYNYMMGVEFNPRIGKWFDFKLFFNGRPGIVAWTLINLSFAAKQQELYGHVTN 269

Query: 243 SMLVNTALMLVYVTKFFWWEAGYWNTMDIAHDRAGFYICWGCLVWVPSVYTSPGMYLVNH 302
           SM++   L  +YV  FFW E  Y  T+DI HD  G+Y+ WG  VW+P +YT  G+YLV H
Sbjct: 270 SMVLVNILQAIYVLDFFWNETWYLKTIDICHDHFGWYLGWGDCVWLPYLYTLQGLYLVYH 329

Query: 303 PVNLGTQLAVYILVAGILCVYINYECDRQRQEFRRKSGKCLVWGKAPSKIVASYTTTSGD 362
           PV L T  A+ +L+ G+L  YI    + Q+  FRR  G+CL+WG+ P  I  SYT+  G 
Sbjct: 330 PVQLPTYYALGVLLLGLLGYYIFRMTNHQKDLFRRTDGRCLIWGRKPKAIECSYTSADGQ 389

Query: 363 TKTSLLLTSGWWGLARHFHYVPEILAAFFWTVPALFNHFLPYFYVIFLTILLFDRAKRDD 422
              S LL SG+WG+ARHF+Y  +++ +  + +     H LPYFY+IF+ ILL  R  RD+
Sbjct: 390 RHHSKLLVSGFWGVARHFNYTGDLMGSLAYCLACGGGHLLPYFYIIFMAILLTHRCLRDE 449

Query: 423 DRCRSKYGKYWKLYCEKVPYKIIPGIY 436
            RC +KYG+ W+ Y   VPY+++PGI+
Sbjct: 450 HRCANKYGRDWEHYTAAVPYRLLPGIF 475

BLAST of ClCG09G001620 vs. ExPASy Swiss-Prot
Match: Q9Z2Z8 (7-dehydrocholesterol reductase OS=Rattus norvegicus OX=10116 GN=Dhcr7 PE=1 SV=1)

HSP 1 Score: 282.3 bits (721), Expect = 9.0e-75
Identity = 157/442 (35.52%), Postives = 246/442 (55.66%), Query Frame = 0

Query: 12  FLTYASMLSLLTLCPPFVILLWYTMVHADGSVSQTWNYL-YQNGFKGFINMW---PPPSA 71
           + +  S++ LL   P   I+ ++ M     S S T   L    G     ++W   PP +A
Sbjct: 33  WFSLVSVIFLLLFAP--FIVYYFIMACDQYSCSLTAPILDVATGRASLADIWAKTPPVTA 92

Query: 72  TACKIIGCYAAFEAALQLLLP-----------GKRVLGPVSPTGNQPVYKANGVAAYIVT 131
            A ++   + +F+  L   LP           G    G ++P G    Y+ NG+ A+++T
Sbjct: 93  KAAQLYALWVSFQVLLYSWLPDFCHRFLPGYVGGVQEGAITPAGIVNKYEVNGLQAWLIT 152

Query: 132 -LVTYTALWWFGIFDPTIVYDH-LGEIFSALIFGSFIFCILLYIKGHVAPSSS-DSGSSG 191
             + +   +    F PTI++D+ +  ++ A I G +       IKG++ P+S+ D   +G
Sbjct: 153 HFLWFVNAYLLSWFSPTIIFDNWIPLLWCANILG-YAVSTFAMIKGYLFPTSAEDCKFTG 212

Query: 192 NIILDFYWGMELYPRIGKHFDIKVFTNCRFGMMSWAVLAVTYCIKQFEKNGTIADSMLVN 251
           N   ++  G+E  PRIGK FD K+F N R G+++W ++ +++  KQ E  G + +SM++ 
Sbjct: 213 NFFYNYMMGIEFNPRIGKWFDFKLFFNGRPGIVAWTLINLSFAAKQQELYGHVTNSMILV 272

Query: 252 TALMLVYVTKFFWWEAGYWNTMDIAHDRAGFYICWGCLVWVPSVYTSPGMYLVNHPVNLG 311
             L  +YV  FFW E  Y  T+DI HD  G+Y+ WG  VW+P +YT  G+YLV HPV L 
Sbjct: 273 NVLQAIYVLDFFWNETWYLKTIDICHDHFGWYLGWGDCVWLPYLYTLQGLYLVYHPVQLS 332

Query: 312 TQLAVYILVAGILCVYINYECDRQRQEFRRKSGKCLVWGKAPSKIVASYTTTSGDTKTSL 371
           T  A+ +L+ G++  YI    + Q+  FRR  G CL+WGK P  I  SYT+  G    S 
Sbjct: 333 TPNALGVLLLGLVGYYIFRMTNHQKDLFRRTDGHCLIWGKKPKAIECSYTSADGLKHRSK 392

Query: 372 LLTSGWWGLARHFHYVPEILAAFFWTVPALFNHFLPYFYVIFLTILLFDRAKRDDDRCRS 431
           LL SG+WG+ARHF+Y  +++ +  + +     H LPYFY+I++TILL  R  RD+ RC +
Sbjct: 393 LLVSGFWGVARHFNYTGDLMGSLAYCLACGGGHLLPYFYIIYMTILLTHRCLRDEHRCAN 452

Query: 432 KYGKYWKLYCEKVPYKIIPGIY 436
           KYG+ W+ Y   VPY+++PGI+
Sbjct: 453 KYGRDWERYVAAVPYRLLPGIF 471

BLAST of ClCG09G001620 vs. ExPASy Swiss-Prot
Match: Q7ZXH1 (7-dehydrocholesterol reductase OS=Xenopus laevis OX=8355 GN=dhcr7 PE=2 SV=1)

HSP 1 Score: 279.6 bits (714), Expect = 5.9e-74
Identity = 156/443 (35.21%), Postives = 246/443 (55.53%), Query Frame = 0

Query: 12  FLTYASMLSLLTLCPPFVILLWYTMVHADG---SVSQTWNYLYQNGFKGFINMW---PPP 71
           + + AS++ LL   P   ++++Y ++  D    +++     LY +G     ++W   P  
Sbjct: 35  YFSLASVIFLLAFAP---LIVYYFVMSCDQYQCALTAPVLDLY-SGKARLSDIWDKTPAL 94

Query: 72  SATACKIIGCYAAFEAALQLLLP-----------GKRVLGPVSPTGNQPVYKANGVAAYI 131
           + TA KI   + +F+  L + LP           G    G  +P G    Y+ NG+ A+ 
Sbjct: 95  TWTAVKIYLAWVSFQVFLYMFLPDILHKFVPGYEGGVQEGARTPAGLINKYQVNGLQAWT 154

Query: 132 VT-LVTYTALWWFGIFDPTIVYDH-LGEIFSALIFGSFIFCILLYIKGHVAPSSSDSGSS 191
           +T L+ +   + F  F PTIV D+ +  ++ A + G  +    L        +++D   +
Sbjct: 155 ITHLLWFANAYHFHWFSPTIVIDNWIPLLWCANLLGYSVATFALVKANFFPTNANDCKFT 214

Query: 192 GNIILDFYWGMELYPRIGKHFDIKVFTNCRFGMMSWAVLAVTYCIKQFEKNGTIADSMLV 251
           GN   D+  G+E  PRIGK FD K+F N R G+++W ++ ++Y  KQ E  G + +SM++
Sbjct: 215 GNFFYDYMMGIEFNPRIGKWFDFKLFFNGRPGIVAWTLINLSYAAKQQELYGQVTNSMIL 274

Query: 252 NTALMLVYVTKFFWWEAGYWNTMDIAHDRAGFYICWGCLVWVPSVYTSPGMYLVNHPVNL 311
              L  +YV  FFW E+ Y  T+DI HD  G+Y+ WG  VW+P +YT  G+YLV +PV L
Sbjct: 275 VNVLQAIYVVDFFWNESWYLKTIDICHDHFGWYLGWGDCVWLPYLYTLQGLYLVYNPVEL 334

Query: 312 GTQLAVYILVAGILCVYINYECDRQRQEFRRKSGKCLVWGKAPSKIVASYTTTSGDTKTS 371
            T  AV +L+ G++  YI    + Q+  FRR +G C +WGK P  I   Y +  G    S
Sbjct: 335 STTAAVAVLLLGLIGYYIFRMTNHQKDLFRRTNGNCKIWGKKPKSIECFYVSADGKRHYS 394

Query: 372 LLLTSGWWGLARHFHYVPEILAAFFWTVPALFNHFLPYFYVIFLTILLFDRAKRDDDRCR 431
            L+ SG+WG+ARH +Y  +++ +  + +   F+H LPYFY I++TILL  R  RD+ RC 
Sbjct: 395 KLMISGFWGVARHLNYTGDLMGSLAYCLACGFDHLLPYFYFIYMTILLVHRCIRDEHRCS 454

Query: 432 SKYGKYWKLYCEKVPYKIIPGIY 436
           SKYGK WKLY   VPY+++PG++
Sbjct: 455 SKYGKDWKLYTSAVPYRLLPGLF 473

BLAST of ClCG09G001620 vs. ExPASy TrEMBL
Match: A0A0A0KC98 (Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_7G447780 PE=3 SV=1)

HSP 1 Score: 887.1 bits (2291), Expect = 3.0e-254
Identity = 412/435 (94.71%), Postives = 428/435 (98.39%), Query Frame = 0

Query: 1   MAEGSNTVHSPFLTYASMLSLLTLCPPFVILLWYTMVHADGSVSQTWNYLYQNGFKGFIN 60
           MAEGSNTVHSPFLTY SMLSLLTLCPPFVILLWYTMVHADGSVSQTWNYLYQNGF+GFIN
Sbjct: 1   MAEGSNTVHSPFLTYVSMLSLLTLCPPFVILLWYTMVHADGSVSQTWNYLYQNGFEGFIN 60

Query: 61  MWPPPSATACKIIGCYAAFEAALQLLLPGKRVLGPVSPTGNQPVYKANGVAAYIVTLVTY 120
           +WP PSATACKIIGCYAAFEAALQLLLPGKRVLGPVSPTGNQPVYKANGVAAYIVTL+TY
Sbjct: 61  IWPSPSATACKIIGCYAAFEAALQLLLPGKRVLGPVSPTGNQPVYKANGVAAYIVTLLTY 120

Query: 121 TALWWFGIFDPTIVYDHLGEIFSALIFGSFIFCILLYIKGHVAPSSSDSGSSGNIILDFY 180
            ALW FGIFDP IVYDHLGEI+S LIFGSFIFCILLYIKGHVAPSSSDSGSSGN+ILDFY
Sbjct: 121 VALWRFGIFDPAIVYDHLGEIYSTLIFGSFIFCILLYIKGHVAPSSSDSGSSGNVILDFY 180

Query: 181 WGMELYPRIGKHFDIKVFTNCRFGMMSWAVLAVTYCIKQFEKNGTIADSMLVNTALMLVY 240
           WGMELYPRIGKHFDIKVFTNCRFGMMSWAVLAVTYCIKQ+EKNGT+ADS+LVNTALMLVY
Sbjct: 181 WGMELYPRIGKHFDIKVFTNCRFGMMSWAVLAVTYCIKQYEKNGTLADSLLVNTALMLVY 240

Query: 241 VTKFFWWEAGYWNTMDIAHDRAGFYICWGCLVWVPSVYTSPGMYLVNHPVNLGTQLAVYI 300
           VTKFFWWEAGYWNTMDIAHDRAGFYICWGCLVWVPSVYTSPGMYLVNHPVNLGTQLAV I
Sbjct: 241 VTKFFWWEAGYWNTMDIAHDRAGFYICWGCLVWVPSVYTSPGMYLVNHPVNLGTQLAVSI 300

Query: 301 LVAGILCVYINYECDRQRQEFRRKSGKCLVWGKAPSKIVASYTTTSGDTKTSLLLTSGWW 360
           LVAGILC+YINY+CDRQRQEFRRK+GKCLVWGKAPSKIVA+YTTT+G+T+TSLLLTSGWW
Sbjct: 301 LVAGILCIYINYDCDRQRQEFRRKNGKCLVWGKAPSKIVATYTTTTGETRTSLLLTSGWW 360

Query: 361 GLARHFHYVPEILAAFFWTVPALFNHFLPYFYVIFLTILLFDRAKRDDDRCRSKYGKYWK 420
           GLARHFHYVPEILAAFFWTVPALF+HFLPYFYVIFLTILLFDRAKRDDDRCRSKYGKYWK
Sbjct: 361 GLARHFHYVPEILAAFFWTVPALFHHFLPYFYVIFLTILLFDRAKRDDDRCRSKYGKYWK 420

Query: 421 LYCEKVPYKIIPGIY 436
           LYCEKVPYKIIPGIY
Sbjct: 421 LYCEKVPYKIIPGIY 435

BLAST of ClCG09G001620 vs. ExPASy TrEMBL
Match: A0A5A7UXH5 (7-dehydrocholesterol reductase OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold347G001860 PE=3 SV=1)

HSP 1 Score: 886.3 bits (2289), Expect = 5.0e-254
Identity = 416/435 (95.63%), Postives = 426/435 (97.93%), Query Frame = 0

Query: 1   MAEGSNTVHSPFLTYASMLSLLTLCPPFVILLWYTMVHADGSVSQTWNYLYQNGFKGFIN 60
           MAEGSNTVHSPFLTY SMLSLLTLCPPFVILLWYTMVHADGSVSQTWNYLYQNGF+GFIN
Sbjct: 1   MAEGSNTVHSPFLTYVSMLSLLTLCPPFVILLWYTMVHADGSVSQTWNYLYQNGFEGFIN 60

Query: 61  MWPPPSATACKIIGCYAAFEAALQLLLPGKRVLGPVSPTGNQPVYKANGVAAYIVTLVTY 120
           +WP PSATACKIIGCYAAFEAALQLLLPGKRVLGPVSPTGNQPVYKANGVAAYIVTLVTY
Sbjct: 61  IWPSPSATACKIIGCYAAFEAALQLLLPGKRVLGPVSPTGNQPVYKANGVAAYIVTLVTY 120

Query: 121 TALWWFGIFDPTIVYDHLGEIFSALIFGSFIFCILLYIKGHVAPSSSDSGSSGNIILDFY 180
            ALW FGIFDP IVYDHLGEIFS LIFGSFIFCILLYIKGHVAPSSSDSGSSGNIILDFY
Sbjct: 121 VALWRFGIFDPAIVYDHLGEIFSTLIFGSFIFCILLYIKGHVAPSSSDSGSSGNIILDFY 180

Query: 181 WGMELYPRIGKHFDIKVFTNCRFGMMSWAVLAVTYCIKQFEKNGTIADSMLVNTALMLVY 240
           WGMELYPRIGKHFDIKVFTNCRFGMMSWAVLAVTYCIKQ+EKNG +ADSMLVNTALMLVY
Sbjct: 181 WGMELYPRIGKHFDIKVFTNCRFGMMSWAVLAVTYCIKQYEKNGILADSMLVNTALMLVY 240

Query: 241 VTKFFWWEAGYWNTMDIAHDRAGFYICWGCLVWVPSVYTSPGMYLVNHPVNLGTQLAVYI 300
           VTKFFWWEAGYWNTMDIAHDRAGFYICWGCLVWVPSVYTSPGMYLVNHPVNLGTQLAV I
Sbjct: 241 VTKFFWWEAGYWNTMDIAHDRAGFYICWGCLVWVPSVYTSPGMYLVNHPVNLGTQLAVSI 300

Query: 301 LVAGILCVYINYECDRQRQEFRRKSGKCLVWGKAPSKIVASYTTTSGDTKTSLLLTSGWW 360
           LVAGILC+YINY+CDRQRQEFRRK+GK LVWGKAPSKIVASYTTTSG+T+TSLLLTSGWW
Sbjct: 301 LVAGILCIYINYDCDRQRQEFRRKNGKRLVWGKAPSKIVASYTTTSGETRTSLLLTSGWW 360

Query: 361 GLARHFHYVPEILAAFFWTVPALFNHFLPYFYVIFLTILLFDRAKRDDDRCRSKYGKYWK 420
           GLARHFHYVPEILAAFFWTVPALF+HFLPYFYVIFLTILLFDRAKRDDDRCRSKYGKYWK
Sbjct: 361 GLARHFHYVPEILAAFFWTVPALFHHFLPYFYVIFLTILLFDRAKRDDDRCRSKYGKYWK 420

Query: 421 LYCEKVPYKIIPGIY 436
           LYCEKVPYKIIPGIY
Sbjct: 421 LYCEKVPYKIIPGIY 435

BLAST of ClCG09G001620 vs. ExPASy TrEMBL
Match: A0A1S3CGG3 (7-dehydrocholesterol reductase OS=Cucumis melo OX=3656 GN=LOC103500620 PE=3 SV=1)

HSP 1 Score: 886.3 bits (2289), Expect = 5.0e-254
Identity = 416/435 (95.63%), Postives = 426/435 (97.93%), Query Frame = 0

Query: 1   MAEGSNTVHSPFLTYASMLSLLTLCPPFVILLWYTMVHADGSVSQTWNYLYQNGFKGFIN 60
           MAEGSNTVHSPFLTY SMLSLLTLCPPFVILLWYTMVHADGSVSQTWNYLYQNGF+GFIN
Sbjct: 1   MAEGSNTVHSPFLTYVSMLSLLTLCPPFVILLWYTMVHADGSVSQTWNYLYQNGFEGFIN 60

Query: 61  MWPPPSATACKIIGCYAAFEAALQLLLPGKRVLGPVSPTGNQPVYKANGVAAYIVTLVTY 120
           +WP PSATACKIIGCYAAFEAALQLLLPGKRVLGPVSPTGNQPVYKANGVAAYIVTLVTY
Sbjct: 61  IWPSPSATACKIIGCYAAFEAALQLLLPGKRVLGPVSPTGNQPVYKANGVAAYIVTLVTY 120

Query: 121 TALWWFGIFDPTIVYDHLGEIFSALIFGSFIFCILLYIKGHVAPSSSDSGSSGNIILDFY 180
            ALW FGIFDP IVYDHLGEIFS LIFGSFIFCILLYIKGHVAPSSSDSGSSGNIILDFY
Sbjct: 121 VALWRFGIFDPAIVYDHLGEIFSTLIFGSFIFCILLYIKGHVAPSSSDSGSSGNIILDFY 180

Query: 181 WGMELYPRIGKHFDIKVFTNCRFGMMSWAVLAVTYCIKQFEKNGTIADSMLVNTALMLVY 240
           WGMELYPRIGKHFDIKVFTNCRFGMMSWAVLAVTYCIKQ+EKNG +ADSMLVNTALMLVY
Sbjct: 181 WGMELYPRIGKHFDIKVFTNCRFGMMSWAVLAVTYCIKQYEKNGILADSMLVNTALMLVY 240

Query: 241 VTKFFWWEAGYWNTMDIAHDRAGFYICWGCLVWVPSVYTSPGMYLVNHPVNLGTQLAVYI 300
           VTKFFWWEAGYWNTMDIAHDRAGFYICWGCLVWVPSVYTSPGMYLVNHPVNLGTQLAV I
Sbjct: 241 VTKFFWWEAGYWNTMDIAHDRAGFYICWGCLVWVPSVYTSPGMYLVNHPVNLGTQLAVSI 300

Query: 301 LVAGILCVYINYECDRQRQEFRRKSGKCLVWGKAPSKIVASYTTTSGDTKTSLLLTSGWW 360
           LVAGILC+YINY+CDRQRQEFRRK+GK LVWGKAPSKIVASYTTTSG+T+TSLLLTSGWW
Sbjct: 301 LVAGILCIYINYDCDRQRQEFRRKNGKRLVWGKAPSKIVASYTTTSGETRTSLLLTSGWW 360

Query: 361 GLARHFHYVPEILAAFFWTVPALFNHFLPYFYVIFLTILLFDRAKRDDDRCRSKYGKYWK 420
           GLARHFHYVPEILAAFFWTVPALF+HFLPYFYVIFLTILLFDRAKRDDDRCRSKYGKYWK
Sbjct: 361 GLARHFHYVPEILAAFFWTVPALFHHFLPYFYVIFLTILLFDRAKRDDDRCRSKYGKYWK 420

Query: 421 LYCEKVPYKIIPGIY 436
           LYCEKVPYKIIPGIY
Sbjct: 421 LYCEKVPYKIIPGIY 435

BLAST of ClCG09G001620 vs. ExPASy TrEMBL
Match: A0A6J1D3V8 (7-dehydrocholesterol reductase OS=Momordica charantia OX=3673 GN=LOC111016744 PE=3 SV=1)

HSP 1 Score: 870.5 bits (2248), Expect = 2.9e-249
Identity = 403/435 (92.64%), Postives = 419/435 (96.32%), Query Frame = 0

Query: 1   MAEGSNTVHSPFLTYASMLSLLTLCPPFVILLWYTMVHADGSVSQTWNYLYQNGFKGFIN 60
           MAEGS TVHSP LTY SMLSLL +CPPFVI LWYTMVHADGSVSQTWNYLY NG +GF+N
Sbjct: 1   MAEGSTTVHSPILTYVSMLSLLIICPPFVIFLWYTMVHADGSVSQTWNYLYHNGVRGFMN 60

Query: 61  MWPPPSATACKIIGCYAAFEAALQLLLPGKRVLGPVSPTGNQPVYKANGVAAYIVTLVTY 120
           +WPPPSATACKIIGCYAAFEAALQLLLPG RV GPVSPTGNQPVYKANGVAAYIVTLVTY
Sbjct: 61  IWPPPSATACKIIGCYAAFEAALQLLLPGNRVQGPVSPTGNQPVYKANGVAAYIVTLVTY 120

Query: 121 TALWWFGIFDPTIVYDHLGEIFSALIFGSFIFCILLYIKGHVAPSSSDSGSSGNIILDFY 180
            ALWWFGIF+PTIVYDHLGEIFSALIFGS IFCI LYIKGHVAPSSSDSGSSGNII+DFY
Sbjct: 121 IALWWFGIFNPTIVYDHLGEIFSALIFGSLIFCIFLYIKGHVAPSSSDSGSSGNIIIDFY 180

Query: 181 WGMELYPRIGKHFDIKVFTNCRFGMMSWAVLAVTYCIKQFEKNGTIADSMLVNTALMLVY 240
           WGMELYPRIGK+FDIKVFTNCRFGMMSWAVLAVTYCIKQ+EKNGTIADSMLVNTALMLVY
Sbjct: 181 WGMELYPRIGKNFDIKVFTNCRFGMMSWAVLAVTYCIKQYEKNGTIADSMLVNTALMLVY 240

Query: 241 VTKFFWWEAGYWNTMDIAHDRAGFYICWGCLVWVPSVYTSPGMYLVNHPVNLGTQLAVYI 300
           VTKFFWWE+GYWNTMDIAHDRAGFYICWGCLVWVPSVYTSPGMYLVNHPVNLGTQLAVYI
Sbjct: 241 VTKFFWWESGYWNTMDIAHDRAGFYICWGCLVWVPSVYTSPGMYLVNHPVNLGTQLAVYI 300

Query: 301 LVAGILCVYINYECDRQRQEFRRKSGKCLVWGKAPSKIVASYTTTSGDTKTSLLLTSGWW 360
           LVAG+LC+YINY+CDRQRQEFRRK+GKCL+W KAPSKIVASYTTTSG+TKTSLLLTSGWW
Sbjct: 301 LVAGLLCIYINYDCDRQRQEFRRKNGKCLIWRKAPSKIVASYTTTSGETKTSLLLTSGWW 360

Query: 361 GLARHFHYVPEILAAFFWTVPALFNHFLPYFYVIFLTILLFDRAKRDDDRCRSKYGKYWK 420
           GLARHFHY PEILAAFFWTVPALFNHFLPYFYVIFLTILL DRAKRDDDRCRSKYGKYWK
Sbjct: 361 GLARHFHYAPEILAAFFWTVPALFNHFLPYFYVIFLTILLLDRAKRDDDRCRSKYGKYWK 420

Query: 421 LYCEKVPYKIIPGIY 436
           LYCEKV Y+IIPGIY
Sbjct: 421 LYCEKVAYRIIPGIY 435

BLAST of ClCG09G001620 vs. ExPASy TrEMBL
Match: A0A6J1HK80 (7-dehydrocholesterol reductase OS=Cucurbita moschata OX=3662 GN=LOC111464317 PE=3 SV=1)

HSP 1 Score: 859.4 bits (2219), Expect = 6.6e-246
Identity = 400/434 (92.17%), Postives = 418/434 (96.31%), Query Frame = 0

Query: 1   MAEGSNTVHSPFLTYASMLSLLTLCPPFVILLWYTMVHADGSVSQTWNYLYQNGFKGFIN 60
           MAEGSNTVHSP +TYASMLSLLTLCPPFVI LWYTMVHADGSVSQT  YLY NG +G I+
Sbjct: 1   MAEGSNTVHSPLVTYASMLSLLTLCPPFVIFLWYTMVHADGSVSQTMAYLYHNGLRGLID 60

Query: 61  MWPPPSATACKIIGCYAAFEAALQLLLPGKRVLGPVSPTGNQPVYKANGVAAYIVTLVTY 120
           +WPPPSATA K+IG YAAFEAALQLLLPGK V GP+SPTGNQPVYKANGVAAY+VTLVTY
Sbjct: 61  IWPPPSATAFKLIGSYAAFEAALQLLLPGKTVYGPISPTGNQPVYKANGVAAYVVTLVTY 120

Query: 121 TALWWFGIFDPTIVYDHLGEIFSALIFGSFIFCILLYIKGHVAPSSSDSGSSGNIILDFY 180
            ALWWFGIF+PT+VYDHLGEIFSALI GSFIFCI+LYIKG VAPSSSDSGSSGNII+DFY
Sbjct: 121 IALWWFGIFNPTVVYDHLGEIFSALIIGSFIFCIVLYIKGCVAPSSSDSGSSGNIIIDFY 180

Query: 181 WGMELYPRIGKHFDIKVFTNCRFGMMSWAVLAVTYCIKQFEKNGTIADSMLVNTALMLVY 240
           WGMELYPRIGKHFDIKVFTNCRFGMMSWAVLAVTYCIKQ+EKNGTIADSMLVNTALML Y
Sbjct: 181 WGMELYPRIGKHFDIKVFTNCRFGMMSWAVLAVTYCIKQYEKNGTIADSMLVNTALMLAY 240

Query: 241 VTKFFWWEAGYWNTMDIAHDRAGFYICWGCLVWVPSVYTSPGMYLVNHPVNLGTQLAVYI 300
           VTKFFWWEAGYWNTMDIAHDRAGFYICWGCLVWVPSVYTSPGMYLVNHPVNLGTQLAVYI
Sbjct: 241 VTKFFWWEAGYWNTMDIAHDRAGFYICWGCLVWVPSVYTSPGMYLVNHPVNLGTQLAVYI 300

Query: 301 LVAGILCVYINYECDRQRQEFRRKSGKCLVWGKAPSKIVASYTTTSGDTKTSLLLTSGWW 360
           LVAG+LC+YINY+CDRQRQEFRRK+GKCLVWGKAPSKIVASYTTTSG+TKTSLLLTSGWW
Sbjct: 301 LVAGMLCIYINYDCDRQRQEFRRKNGKCLVWGKAPSKIVASYTTTSGETKTSLLLTSGWW 360

Query: 361 GLARHFHYVPEILAAFFWTVPALFNHFLPYFYVIFLTILLFDRAKRDDDRCRSKYGKYWK 420
           GLARHFHYVPEILAAFFWTVPALFNHFLPYFYVIFLTILL DRAKRDDDRCRSKYGKYWK
Sbjct: 361 GLARHFHYVPEILAAFFWTVPALFNHFLPYFYVIFLTILLVDRAKRDDDRCRSKYGKYWK 420

Query: 421 LYCEKVPYKIIPGI 435
           LYCEKVPY+IIPGI
Sbjct: 421 LYCEKVPYRIIPGI 434

BLAST of ClCG09G001620 vs. TAIR 10
Match: AT1G50430.1 (Ergosterol biosynthesis ERG4/ERG24 family )

HSP 1 Score: 802.4 bits (2071), Expect = 1.8e-232
Identity = 368/431 (85.38%), Postives = 399/431 (92.58%), Query Frame = 0

Query: 5   SNTVHSPFLTYASMLSLLTLCPPFVILLWYTMVHADGSVSQTWNYLYQNGFKGFINMWPP 64
           + TVHSP +TYASMLSLL  CPPFVILLWYTMVH DGSV+QT+ + ++NG +G IN+WP 
Sbjct: 2   AETVHSPIVTYASMLSLLAFCPPFVILLWYTMVHQDGSVTQTFGFFWENGVQGLINIWPR 61

Query: 65  PSATACKIIGCYAAFEAALQLLLPGKRVLGPVSPTGNQPVYKANGVAAYIVTLVTYTALW 124
           P+  A KII CY AFEA LQLLLPGKRV GP+SP GN+PVYKANG+AAY VTL TY  LW
Sbjct: 62  PTLIAWKIIFCYGAFEAILQLLLPGKRVEGPISPAGNRPVYKANGLAAYFVTLATYLGLW 121

Query: 125 WFGIFDPTIVYDHLGEIFSALIFGSFIFCILLYIKGHVAPSSSDSGSSGNIILDFYWGME 184
           WFGIF+P IVYDHLGEIFSALIFGSFIFC+LLYIKGHVAPSSSDSGS GN+I+DFYWGME
Sbjct: 122 WFGIFNPAIVYDHLGEIFSALIFGSFIFCVLLYIKGHVAPSSSDSGSCGNLIIDFYWGME 181

Query: 185 LYPRIGKHFDIKVFTNCRFGMMSWAVLAVTYCIKQFEKNGTIADSMLVNTALMLVYVTKF 244
           LYPRIGK FDIKVFTNCRFGMMSWAVLAVTYCIKQ+E NG ++DSMLVNT LMLVYVTKF
Sbjct: 182 LYPRIGKSFDIKVFTNCRFGMMSWAVLAVTYCIKQYEINGKVSDSMLVNTILMLVYVTKF 241

Query: 245 FWWEAGYWNTMDIAHDRAGFYICWGCLVWVPSVYTSPGMYLVNHPVNLGTQLAVYILVAG 304
           FWWEAGYWNTMDIAHDRAGFYICWGCLVWVPSVYTSPGMYLVNHPV LGTQLA+YILVAG
Sbjct: 242 FWWEAGYWNTMDIAHDRAGFYICWGCLVWVPSVYTSPGMYLVNHPVELGTQLAIYILVAG 301

Query: 305 ILCVYINYECDRQRQEFRRKSGKCLVWGKAPSKIVASYTTTSGDTKTSLLLTSGWWGLAR 364
           ILC+YINY+CDRQRQEFRR +GKCLVWG+APSKIVASYTTTSG+TKTSLLLTSGWWGLAR
Sbjct: 302 ILCIYINYDCDRQRQEFRRTNGKCLVWGRAPSKIVASYTTTSGETKTSLLLTSGWWGLAR 361

Query: 365 HFHYVPEILAAFFWTVPALFNHFLPYFYVIFLTILLFDRAKRDDDRCRSKYGKYWKLYCE 424
           HFHYVPEIL+AFFWTVPALF++FL YFYVIFLT+LLFDRAKRDDDRCRSKYGKYWKLYCE
Sbjct: 362 HFHYVPEILSAFFWTVPALFDNFLAYFYVIFLTLLLFDRAKRDDDRCRSKYGKYWKLYCE 421

Query: 425 KVPYKIIPGIY 436
           KV Y+IIPGIY
Sbjct: 422 KVKYRIIPGIY 432

BLAST of ClCG09G001620 vs. TAIR 10
Match: AT1G50430.2 (Ergosterol biosynthesis ERG4/ERG24 family )

HSP 1 Score: 793.9 bits (2049), Expect = 6.6e-230
Identity = 367/431 (85.15%), Postives = 398/431 (92.34%), Query Frame = 0

Query: 5   SNTVHSPFLTYASMLSLLTLCPPFVILLWYTMVHADGSVSQTWNYLYQNGFKGFINMWPP 64
           + TVHSP +TYASMLSLL  CPPFVILLWYTMVH DGSV+QT+ + ++NG +G IN+WP 
Sbjct: 2   AETVHSPIVTYASMLSLLAFCPPFVILLWYTMVHQDGSVTQTFGFFWENGVQGLINIWPR 61

Query: 65  PSATACKIIGCYAAFEAALQLLLPGKRVLGPVSPTGNQPVYKANGVAAYIVTLVTYTALW 124
           P+  A KII CY AFEA LQLLLPGKRV GP+SP GN+PVYKANG+AAY VTL TY  L 
Sbjct: 62  PTLIAWKIIFCYGAFEAILQLLLPGKRVEGPISPAGNRPVYKANGLAAYFVTLATYLGL- 121

Query: 125 WFGIFDPTIVYDHLGEIFSALIFGSFIFCILLYIKGHVAPSSSDSGSSGNIILDFYWGME 184
           WFGIF+P IVYDHLGEIFSALIFGSFIFC+LLYIKGHVAPSSSDSGS GN+I+DFYWGME
Sbjct: 122 WFGIFNPAIVYDHLGEIFSALIFGSFIFCVLLYIKGHVAPSSSDSGSCGNLIIDFYWGME 181

Query: 185 LYPRIGKHFDIKVFTNCRFGMMSWAVLAVTYCIKQFEKNGTIADSMLVNTALMLVYVTKF 244
           LYPRIGK FDIKVFTNCRFGMMSWAVLAVTYCIKQ+E NG ++DSMLVNT LMLVYVTKF
Sbjct: 182 LYPRIGKSFDIKVFTNCRFGMMSWAVLAVTYCIKQYEINGKVSDSMLVNTILMLVYVTKF 241

Query: 245 FWWEAGYWNTMDIAHDRAGFYICWGCLVWVPSVYTSPGMYLVNHPVNLGTQLAVYILVAG 304
           FWWEAGYWNTMDIAHDRAGFYICWGCLVWVPSVYTSPGMYLVNHPV LGTQLA+YILVAG
Sbjct: 242 FWWEAGYWNTMDIAHDRAGFYICWGCLVWVPSVYTSPGMYLVNHPVELGTQLAIYILVAG 301

Query: 305 ILCVYINYECDRQRQEFRRKSGKCLVWGKAPSKIVASYTTTSGDTKTSLLLTSGWWGLAR 364
           ILC+YINY+CDRQRQEFRR +GKCLVWG+APSKIVASYTTTSG+TKTSLLLTSGWWGLAR
Sbjct: 302 ILCIYINYDCDRQRQEFRRTNGKCLVWGRAPSKIVASYTTTSGETKTSLLLTSGWWGLAR 361

Query: 365 HFHYVPEILAAFFWTVPALFNHFLPYFYVIFLTILLFDRAKRDDDRCRSKYGKYWKLYCE 424
           HFHYVPEIL+AFFWTVPALF++FL YFYVIFLT+LLFDRAKRDDDRCRSKYGKYWKLYCE
Sbjct: 362 HFHYVPEILSAFFWTVPALFDNFLAYFYVIFLTLLLFDRAKRDDDRCRSKYGKYWKLYCE 421

Query: 425 KVPYKIIPGIY 436
           KV Y+IIPGIY
Sbjct: 422 KVKYRIIPGIY 431

BLAST of ClCG09G001620 vs. TAIR 10
Match: AT3G52940.1 (Ergosterol biosynthesis ERG4/ERG24 family )

HSP 1 Score: 186.4 bits (472), Expect = 4.8e-47
Identity = 118/381 (30.97%), Postives = 202/381 (53.02%), Query Frame = 0

Query: 65  PSATACKIIGCYAAFEAALQLLLPGKRVLGPVSPTGNQPVYKANGVAAYIVTLVTYTALW 124
           PS  +  ++  Y  + A    +LPGK + G +   G+Q  Y+ NG+ A I+ +       
Sbjct: 12  PSLQSVYVLVFYFVYLAVAGEILPGKVIRGVLLSDGSQLRYRCNGLLALILLVAILGICA 71

Query: 125 WFGIFDPTIVYDHLGEIFSALIFGSFIFCIL----LYIKGHVAPSSSDSGS------SGN 184
             GI  P +V D   E+ SA    +FIFC+L    LY+ G    SSS+ GS      SGN
Sbjct: 72  KLGIVSPLVVADRGLELLSA----TFIFCVLVTLALYVTGR---SSSNKGSSLKPHVSGN 131

Query: 185 IILDFYWGMELYPRIGKHFDIKVFTNCRFGMMSWAVLAVTYCIKQFEKNGTIADSMLVNT 244
           ++ D+++G++L P+     D+K F   R GMM W ++ ++   K  + +G+++ SM++  
Sbjct: 132 LVHDWWFGIQLNPQF-MSIDLKFFF-VRAGMMGWLLINLSILAKSVQ-DGSLSQSMILYQ 191

Query: 245 ALMLVYVTKFFWWEAGYWNTMDIAHDRAGFYICWGCLVWVPSVYTSPGMYLVNHPVNLGT 304
               +Y+  +F  E    +T DI  +R GF + +G L+W+P  ++  G +L+++ V L T
Sbjct: 192 IFCALYILDYFVHEEYMTSTWDIIAERLGFMLVFGDLLWIPFTFSIQGWWLLHNKVEL-T 251

Query: 305 QLAVYILVAGILCVYINYECDRQRQEFRRKSGKCLVWGKAPSKIVASYTTTSGDTKTSLL 364
             A+ +     L  Y+ +    +++   +K+ K  +WGK P  +               L
Sbjct: 252 VPAIVVNCLVFLIGYMVFRGANKQKHIFKKNPKTPIWGKPPVVVGGK------------L 311

Query: 365 LTSGWWGLARHFHYVPEILAAFFWTVPALFNHFLPYFYVIFLTILLFDRAKRDDDRCRSK 424
           L SG+WG+ARH +Y+ +++ A  +++P   +  +PYFY I+L ILL  R +RD+ RC  K
Sbjct: 312 LVSGYWGIARHCNYLGDLMLALSFSLPCGISSPVPYFYPIYLLILLIWRERRDEVRCAEK 369

Query: 425 YGKYWKLYCEKVPYKIIPGIY 436
           Y + W  Y   VP++I+P +Y
Sbjct: 372 YKEIWAEYLRLVPWRILPYVY 369

BLAST of ClCG09G001620 vs. TAIR 10
Match: AT3G52940.2 (Ergosterol biosynthesis ERG4/ERG24 family )

HSP 1 Score: 102.4 bits (254), Expect = 9.1e-22
Identity = 69/225 (30.67%), Postives = 118/225 (52.44%), Query Frame = 0

Query: 65  PSATACKIIGCYAAFEAALQLLLPGKRVLGPVSPTGNQPVYKANGVAAYIVTLVTYTALW 124
           PS  +  ++  Y  + A    +LPGK + G +   G+Q  Y+ NG+ A I+ +       
Sbjct: 12  PSLQSVYVLVFYFVYLAVAGEILPGKVIRGVLLSDGSQLRYRCNGLLALILLVAILGICA 71

Query: 125 WFGIFDPTIVYDHLGEIFSALIFGSFIFCIL----LYIKGHVAPSSSDSGS------SGN 184
             GI  P +V D   E+ SA    +FIFC+L    LY+ G    SSS+ GS      SGN
Sbjct: 72  KLGIVSPLVVADRGLELLSA----TFIFCVLVTLALYVTGR---SSSNKGSSLKPHVSGN 131

Query: 185 IILDFYWGMELYPRIGKHFDIKVFTNCRFGMMSWAVLAVTYCIKQFEKNGTIADSMLVNT 244
           ++ D+++G++L P+     D+K F   R GMM W ++ ++   K  + +G+++ SM++  
Sbjct: 132 LVHDWWFGIQLNPQF-MSIDLKFFF-VRAGMMGWLLINLSILAKSVQ-DGSLSQSMILYQ 191

Query: 245 ALMLVYVTKFFWWEAGYWNTMDIAHDRAGFYICWGCLVWVPSVYT 280
               +Y+  +F  E    +T DI  +R GF + +G L+W+P  ++
Sbjct: 192 IFCALYILDYFVHEEYMTSTWDIIAERLGFMLVFGDLLWIPFTFS 226

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
XP_011659586.16.1e-25494.717-dehydrocholesterol reductase isoform X1 [Cucumis sativus] >KGN45416.1 hypothet... [more]
XP_008462206.11.0e-25395.63PREDICTED: 7-dehydrocholesterol reductase [Cucumis melo] >KAA0059337.1 7-dehydro... [more]
XP_038898375.14.0e-25395.637-dehydrocholesterol reductase isoform X1 [Benincasa hispida][more]
XP_022147941.15.9e-24992.647-dehydrocholesterol reductase [Momordica charantia][more]
XP_022964233.11.4e-24592.177-dehydrocholesterol reductase [Cucurbita moschata][more]
Match NameE-valueIdentityDescription
Q9LDU62.6e-23185.387-dehydrocholesterol reductase OS=Arabidopsis thaliana OX=3702 GN=DWF5 PE=1 SV=1[more]
O884556.3e-7635.977-dehydrocholesterol reductase OS=Mus musculus OX=10090 GN=Dhcr7 PE=1 SV=1[more]
Q5E9J59.0e-7537.737-dehydrocholesterol reductase OS=Bos taurus OX=9913 GN=DHCR7 PE=2 SV=1[more]
Q9Z2Z89.0e-7535.527-dehydrocholesterol reductase OS=Rattus norvegicus OX=10116 GN=Dhcr7 PE=1 SV=1[more]
Q7ZXH15.9e-7435.217-dehydrocholesterol reductase OS=Xenopus laevis OX=8355 GN=dhcr7 PE=2 SV=1[more]
Match NameE-valueIdentityDescription
A0A0A0KC983.0e-25494.71Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_7G447780 PE=3 SV=1[more]
A0A5A7UXH55.0e-25495.637-dehydrocholesterol reductase OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_s... [more]
A0A1S3CGG35.0e-25495.637-dehydrocholesterol reductase OS=Cucumis melo OX=3656 GN=LOC103500620 PE=3 SV=1[more]
A0A6J1D3V82.9e-24992.647-dehydrocholesterol reductase OS=Momordica charantia OX=3673 GN=LOC111016744 PE... [more]
A0A6J1HK806.6e-24692.177-dehydrocholesterol reductase OS=Cucurbita moschata OX=3662 GN=LOC111464317 PE=... [more]
Match NameE-valueIdentityDescription
AT1G50430.11.8e-23285.38Ergosterol biosynthesis ERG4/ERG24 family [more]
AT1G50430.26.6e-23085.15Ergosterol biosynthesis ERG4/ERG24 family [more]
AT3G52940.14.8e-4730.97Ergosterol biosynthesis ERG4/ERG24 family [more]
AT3G52940.29.1e-2230.67Ergosterol biosynthesis ERG4/ERG24 family [more]
InterPro
Analysis Name: InterPro Annotations of Watermelon (Charleston Gray) v2.5
Date Performed: 2022-01-31
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR001171Ergosterol biosynthesis ERG4/ERG24PFAMPF01222ERG4_ERG24coord: 54..435
e-value: 4.4E-85
score: 286.0
NoneNo IPR availableGENE3D1.20.120.1630coord: 225..434
e-value: 2.3E-17
score: 65.4
NoneNo IPR availablePANTHERPTHR21257DELTA(14)-STEROL REDUCTASEcoord: 6..435
NoneNo IPR availablePANTHERPTHR21257:SF537-DEHYDROCHOLESTEROL REDUCTASEcoord: 6..435
IPR018083Sterol reductase, conserved sitePROSITEPS01018STEROL_REDUCT_2coord: 399..422

Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
ClCG09G001620.1ClCG09G001620.1mRNA


GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
biological_process GO:0016132 brassinosteroid biosynthetic process
biological_process GO:0016126 sterol biosynthetic process
cellular_component GO:0030176 integral component of endoplasmic reticulum membrane
cellular_component GO:0016020 membrane
molecular_function GO:0016628 oxidoreductase activity, acting on the CH-CH group of donors, NAD or NADP as acceptor
molecular_function GO:0009918 sterol delta7 reductase activity