ClCG09G000780 (gene) Watermelon (Charleston Gray) v2.5

Overview
NameClCG09G000780
Typegene
OrganismCitrullus lanatus subsp. vulgaris cv. Charleston Gray (Watermelon (Charleston Gray) v2.5)
DescriptionacidPPc domain-containing protein
LocationCG_Chr09: 668449 .. 669536 (+)
RNA-Seq ExpressionClCG09G000780
SyntenyClCG09G000780
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: exonCDSpolypeptide
Hold the cursor over a type above to highlight its positions in the sequence below.
ATGTTTAATCTTGGGCCAGAACTGGGCTCTATAAAGATTTCGTTAGAACGAAACGACAAGTCGTCTCGCAATCGGAGCTGTCGAGACGTATATACCGTCTTTTAGTTTCGTCGACGATGGGGAAACTGGAAAGTGACAGTCCAGCGAACGGACACGAGTGAGTGAGTGAGACCAGGTTAATCAATCCCGCGGAAAAGCTCGAATGGAGGAGCCAAAGGCTGAGATCAAAGATTCATCCTCAGGTATTTCCAAACTTTCAAATCGTTCTCGTCAACTTCATTTTAGGCACTATCGTTTTGATTGCTCTGATGTTATGTGCGTTTCTTCAATATGCTGTTTCTGAAATAAAACGAGCTTGAAATTGTTGCACTAAGTAAAGATCATCCAGTGGCATACAGTTGATAACGTATTTCAGCTTTTTCAGTGATTTGTTGTCGGCGAGTGTTGCGCTATCGAGCTAAAAAACACTTCACAGCCCATATTTTCAGTAATTGTTGGCGTTGATTGGTTACTGCGGCTATTTCTAAGGCAATGTTTGGTTTCAAATCATTAGCATGGTTTAGGTTTTTAGGATTAATTGATTCAATGGGAATTGTCAATTATGTTTAGTTGAAAGGAATCTGCTGTCATTTCTGAAGAGATACTTTTCCTTCTGCGCTTATTAGAGCAAATTATTTTCAAAATGGATGAACTTCGAACTTATGGTTATTAAGGTGTTGCTCCAATTTTATGAGGAGACTTTTTAATTCAGAAACGACTTGTCTCTGTTAGAGATTCATAGTTTGATTTTGCTCGAGGTGTTTGGCCGACTGACATTATATATATATTTTCTTCTTGGCGTGCAATGAGAAGAAGAAGATGCAAAATCGCCCAATATTTTTGAAAGAGCGAAAGAAGAGATAGAGGCTGTGATCCACTCTAATAAATCACCACGGCATCACAAAGAAACACATGGAAGAAGCAATGATATTGACGACACTACGCCCGCCGATGAAGTAAAGGCCCCCGGAGTGTTTGAGAGAGTCAAGGAAGAGGTTGAGGCCCTGGTGGGAGCAATTCATCCCAAGAAGGATTCCGGCAATCATTAG

mRNA sequence

ATGTTTAATCTTGGGCCAGAACTGGGCTCTATAAAGATTTCGTTAGAACGAAACGACAAGTCGTCTCGCAATCGGAGCTGTCGAGACGTTAATCAATCCCGCGGAAAAGCTCGAATGGAGGAGCCAAAGGCTGAGATCAAAGATTCATCCTCAGAAGAAGATGCAAAATCGCCCAATATTTTTGAAAGAGCGAAAGAAGAGATAGAGGCTGTGATCCACTCTAATAAATCACCACGGCATCACAAAGAAACACATGGAAGAAGCAATGATATTGACGACACTACGCCCGCCGATGAAGTAAAGGCCCCCGGAGTGTTTGAGAGAGTCAAGGAAGAGGTTGAGGCCCTGGTGGGAGCAATTCATCCCAAGAAGGATTCCGGCAATCATTAG

Coding sequence (CDS)

ATGTTTAATCTTGGGCCAGAACTGGGCTCTATAAAGATTTCGTTAGAACGAAACGACAAGTCGTCTCGCAATCGGAGCTGTCGAGACGTTAATCAATCCCGCGGAAAAGCTCGAATGGAGGAGCCAAAGGCTGAGATCAAAGATTCATCCTCAGAAGAAGATGCAAAATCGCCCAATATTTTTGAAAGAGCGAAAGAAGAGATAGAGGCTGTGATCCACTCTAATAAATCACCACGGCATCACAAAGAAACACATGGAAGAAGCAATGATATTGACGACACTACGCCCGCCGATGAAGTAAAGGCCCCCGGAGTGTTTGAGAGAGTCAAGGAAGAGGTTGAGGCCCTGGTGGGAGCAATTCATCCCAAGAAGGATTCCGGCAATCATTAG

Protein sequence

MFNLGPELGSIKISLERNDKSSRNRSCRDVNQSRGKARMEEPKAEIKDSSSEEDAKSPNIFERAKEEIEAVIHSNKSPRHHKETHGRSNDIDDTTPADEVKAPGVFERVKEEVEALVGAIHPKKDSGNH
Homology
BLAST of ClCG09G000780 vs. NCBI nr
Match: XP_038900254.1 (uncharacterized protein LOC120087339 [Benincasa hispida] >XP_038900255.1 uncharacterized protein LOC120087339 [Benincasa hispida])

HSP 1 Score: 166.4 bits (420), Expect = 1.6e-37
Identity = 84/91 (92.31%), Postives = 88/91 (96.70%), Query Frame = 0

Query: 39  MEEPKAEIKDSSSEEDAKSPNIFERAKEEIEAVIHSNKSPRHHKETHGRSNDIDDTTPAD 98
           MEEPK EIKDSSSE+DAKSPNIFERAKEEIEAVIHSNKSPRHHKETHGRS+DID+TTPAD
Sbjct: 1   MEEPKTEIKDSSSEKDAKSPNIFERAKEEIEAVIHSNKSPRHHKETHGRSDDIDETTPAD 60

Query: 99  EVKAPGVFERVKEEVEALVGAIHPKKDSGNH 130
           EVKAPGVF RVKEEVEAL+GAIHPKKDS NH
Sbjct: 61  EVKAPGVFGRVKEEVEALIGAIHPKKDSDNH 91

BLAST of ClCG09G000780 vs. NCBI nr
Match: XP_008462344.1 (PREDICTED: uncharacterized protein LOC103500717 [Cucumis melo] >KAA0059422.1 uncharacterized protein E6C27_scaffold242G001490 [Cucumis melo var. makuwa] >TYK03904.1 uncharacterized protein E5676_scaffold347G001000 [Cucumis melo var. makuwa])

HSP 1 Score: 159.1 bits (401), Expect = 2.6e-35
Identity = 81/91 (89.01%), Postives = 86/91 (94.51%), Query Frame = 0

Query: 39  MEEPKAEIKDSSSEEDAKSPNIFERAKEEIEAVIHSNKSPRHHKETHGRSNDIDDTTPAD 98
           MEEPKAEIK SSSE++ KSPNIFERAKEEIEAV H NKSPRHHKETHGRS+DID+TTPAD
Sbjct: 1   MEEPKAEIKGSSSEKEPKSPNIFERAKEEIEAVTHCNKSPRHHKETHGRSDDIDETTPAD 60

Query: 99  EVKAPGVFERVKEEVEALVGAIHPKKDSGNH 130
           EVKAPGVF RVKEEVEALVGAIHPKKDSG+H
Sbjct: 61  EVKAPGVFGRVKEEVEALVGAIHPKKDSGSH 91

BLAST of ClCG09G000780 vs. NCBI nr
Match: KGN45517.1 (hypothetical protein Csa_016837 [Cucumis sativus])

HSP 1 Score: 156.0 bits (393), Expect = 2.2e-34
Identity = 80/91 (87.91%), Postives = 84/91 (92.31%), Query Frame = 0

Query: 39  MEEPKAEIKDSSSEEDAKSPNIFERAKEEIEAVIHSNKSPRHHKETHGRSNDIDDTTPAD 98
           MEE K EIK SSSE + KSPNIFERAKEEIEAVIH NKSPRHHKETHGRS+DID+TTPAD
Sbjct: 1   MEESKTEIKGSSSEREPKSPNIFERAKEEIEAVIHCNKSPRHHKETHGRSDDIDETTPAD 60

Query: 99  EVKAPGVFERVKEEVEALVGAIHPKKDSGNH 130
           EVKAPGVF RVKEEVEALVGAIHPKKDSG+H
Sbjct: 61  EVKAPGVFGRVKEEVEALVGAIHPKKDSGSH 91

BLAST of ClCG09G000780 vs. NCBI nr
Match: XP_022144348.1 (uncharacterized protein LOC111014044 isoform X2 [Momordica charantia])

HSP 1 Score: 155.6 bits (392), Expect = 2.9e-34
Identity = 76/91 (83.52%), Postives = 85/91 (93.41%), Query Frame = 0

Query: 39  MEEPKAEIKDSSSEEDAKSPNIFERAKEEIEAVIHSNKSPRHHKETHGRSNDIDDTTPAD 98
           MEEPK  IKDSSS +DAK+PN+FERAKEEIEA++HSNKSPRHHKETHGRS+DID+TTP+D
Sbjct: 1   MEEPKTVIKDSSSGKDAKAPNLFERAKEEIEAIVHSNKSPRHHKETHGRSDDIDETTPSD 60

Query: 99  EVKAPGVFERVKEEVEALVGAIHPKKDSGNH 130
           EVKAPGVF RVKEE+EALVG IHPKKDS NH
Sbjct: 61  EVKAPGVFGRVKEEIEALVGTIHPKKDSDNH 91

BLAST of ClCG09G000780 vs. NCBI nr
Match: XP_022144340.1 (uncharacterized protein LOC111014044 isoform X1 [Momordica charantia])

HSP 1 Score: 153.7 bits (387), Expect = 1.1e-33
Identity = 77/92 (83.70%), Postives = 86/92 (93.48%), Query Frame = 0

Query: 39  MEEPKAEIKDSSSE-EDAKSPNIFERAKEEIEAVIHSNKSPRHHKETHGRSNDIDDTTPA 98
           MEEPK  IKDSSSE +DAK+PN+FERAKEEIEA++HSNKSPRHHKETHGRS+DID+TTP+
Sbjct: 1   MEEPKTVIKDSSSEGKDAKAPNLFERAKEEIEAIVHSNKSPRHHKETHGRSDDIDETTPS 60

Query: 99  DEVKAPGVFERVKEEVEALVGAIHPKKDSGNH 130
           DEVKAPGVF RVKEE+EALVG IHPKKDS NH
Sbjct: 61  DEVKAPGVFGRVKEEIEALVGTIHPKKDSDNH 92

BLAST of ClCG09G000780 vs. ExPASy TrEMBL
Match: A0A5D3BY12 (Uncharacterized protein OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold347G001000 PE=4 SV=1)

HSP 1 Score: 159.1 bits (401), Expect = 1.3e-35
Identity = 81/91 (89.01%), Postives = 86/91 (94.51%), Query Frame = 0

Query: 39  MEEPKAEIKDSSSEEDAKSPNIFERAKEEIEAVIHSNKSPRHHKETHGRSNDIDDTTPAD 98
           MEEPKAEIK SSSE++ KSPNIFERAKEEIEAV H NKSPRHHKETHGRS+DID+TTPAD
Sbjct: 1   MEEPKAEIKGSSSEKEPKSPNIFERAKEEIEAVTHCNKSPRHHKETHGRSDDIDETTPAD 60

Query: 99  EVKAPGVFERVKEEVEALVGAIHPKKDSGNH 130
           EVKAPGVF RVKEEVEALVGAIHPKKDSG+H
Sbjct: 61  EVKAPGVFGRVKEEVEALVGAIHPKKDSGSH 91

BLAST of ClCG09G000780 vs. ExPASy TrEMBL
Match: A0A1S3CGT3 (uncharacterized protein LOC103500717 OS=Cucumis melo OX=3656 GN=LOC103500717 PE=4 SV=1)

HSP 1 Score: 159.1 bits (401), Expect = 1.3e-35
Identity = 81/91 (89.01%), Postives = 86/91 (94.51%), Query Frame = 0

Query: 39  MEEPKAEIKDSSSEEDAKSPNIFERAKEEIEAVIHSNKSPRHHKETHGRSNDIDDTTPAD 98
           MEEPKAEIK SSSE++ KSPNIFERAKEEIEAV H NKSPRHHKETHGRS+DID+TTPAD
Sbjct: 1   MEEPKAEIKGSSSEKEPKSPNIFERAKEEIEAVTHCNKSPRHHKETHGRSDDIDETTPAD 60

Query: 99  EVKAPGVFERVKEEVEALVGAIHPKKDSGNH 130
           EVKAPGVF RVKEEVEALVGAIHPKKDSG+H
Sbjct: 61  EVKAPGVFGRVKEEVEALVGAIHPKKDSGSH 91

BLAST of ClCG09G000780 vs. ExPASy TrEMBL
Match: A0A0A0KAY9 (Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_7G450740 PE=4 SV=1)

HSP 1 Score: 156.0 bits (393), Expect = 1.1e-34
Identity = 80/91 (87.91%), Postives = 84/91 (92.31%), Query Frame = 0

Query: 39  MEEPKAEIKDSSSEEDAKSPNIFERAKEEIEAVIHSNKSPRHHKETHGRSNDIDDTTPAD 98
           MEE K EIK SSSE + KSPNIFERAKEEIEAVIH NKSPRHHKETHGRS+DID+TTPAD
Sbjct: 1   MEESKTEIKGSSSEREPKSPNIFERAKEEIEAVIHCNKSPRHHKETHGRSDDIDETTPAD 60

Query: 99  EVKAPGVFERVKEEVEALVGAIHPKKDSGNH 130
           EVKAPGVF RVKEEVEALVGAIHPKKDSG+H
Sbjct: 61  EVKAPGVFGRVKEEVEALVGAIHPKKDSGSH 91

BLAST of ClCG09G000780 vs. ExPASy TrEMBL
Match: A0A6J1CT17 (uncharacterized protein LOC111014044 isoform X2 OS=Momordica charantia OX=3673 GN=LOC111014044 PE=4 SV=1)

HSP 1 Score: 155.6 bits (392), Expect = 1.4e-34
Identity = 76/91 (83.52%), Postives = 85/91 (93.41%), Query Frame = 0

Query: 39  MEEPKAEIKDSSSEEDAKSPNIFERAKEEIEAVIHSNKSPRHHKETHGRSNDIDDTTPAD 98
           MEEPK  IKDSSS +DAK+PN+FERAKEEIEA++HSNKSPRHHKETHGRS+DID+TTP+D
Sbjct: 1   MEEPKTVIKDSSSGKDAKAPNLFERAKEEIEAIVHSNKSPRHHKETHGRSDDIDETTPSD 60

Query: 99  EVKAPGVFERVKEEVEALVGAIHPKKDSGNH 130
           EVKAPGVF RVKEE+EALVG IHPKKDS NH
Sbjct: 61  EVKAPGVFGRVKEEIEALVGTIHPKKDSDNH 91

BLAST of ClCG09G000780 vs. ExPASy TrEMBL
Match: A0A6J1CRC8 (uncharacterized protein LOC111014044 isoform X1 OS=Momordica charantia OX=3673 GN=LOC111014044 PE=4 SV=1)

HSP 1 Score: 153.7 bits (387), Expect = 5.3e-34
Identity = 77/92 (83.70%), Postives = 86/92 (93.48%), Query Frame = 0

Query: 39  MEEPKAEIKDSSSE-EDAKSPNIFERAKEEIEAVIHSNKSPRHHKETHGRSNDIDDTTPA 98
           MEEPK  IKDSSSE +DAK+PN+FERAKEEIEA++HSNKSPRHHKETHGRS+DID+TTP+
Sbjct: 1   MEEPKTVIKDSSSEGKDAKAPNLFERAKEEIEAIVHSNKSPRHHKETHGRSDDIDETTPS 60

Query: 99  DEVKAPGVFERVKEEVEALVGAIHPKKDSGNH 130
           DEVKAPGVF RVKEE+EALVG IHPKKDS NH
Sbjct: 61  DEVKAPGVFGRVKEEIEALVGTIHPKKDSDNH 92

BLAST of ClCG09G000780 vs. TAIR 10
Match: AT1G72690.1 (unknown protein; BEST Arabidopsis thaliana protein match is: unknown protein (TAIR:AT1G17490.1); Has 59 Blast hits to 45 proteins in 9 species: Archae - 0; Bacteria - 0; Metazoa - 0; Fungi - 0; Plants - 59; Viruses - 0; Other Eukaryotes - 0 (source: NCBI BLink). )

HSP 1 Score: 90.1 bits (222), Expect = 1.4e-18
Identity = 48/82 (58.54%), Postives = 62/82 (75.61%), Query Frame = 0

Query: 47  KDSSSEEDAKSPNIFERAKEEIEAVIHSNKS-PRHH-KETHGRSNDIDDTTPADEVKAPG 106
           ++ S E+  KSPN+FER KEEIEA+ H  KS  RHH KETHG S+DID++TP D VK PG
Sbjct: 4   QNRSEEKTVKSPNVFERVKEEIEAMGHHEKSKSRHHDKETHGTSDDIDESTPIDYVKGPG 63

Query: 107 VFERVKEEVEALVGAIHPKKDS 127
            F+R+KEE+EA+  A+ PK+ S
Sbjct: 64  FFQRLKEEIEAIFNAVTPKRSS 85

BLAST of ClCG09G000780 vs. TAIR 10
Match: AT5G57000.1 (unknown protein; FUNCTIONS IN: molecular_function unknown; INVOLVED IN: biological_process unknown; LOCATED IN: cellular_component unknown; EXPRESSED IN: 24 plant structures; EXPRESSED DURING: 15 growth stages; BEST Arabidopsis thaliana protein match is: unknown protein (TAIR:AT1G72690.1); Has 153 Blast hits to 116 proteins in 29 species: Archae - 0; Bacteria - 6; Metazoa - 33; Fungi - 7; Plants - 82; Viruses - 0; Other Eukaryotes - 25 (source: NCBI BLink). )

HSP 1 Score: 85.5 bits (210), Expect = 3.4e-17
Identity = 43/91 (47.25%), Postives = 60/91 (65.93%), Query Frame = 0

Query: 38  RMEEPKAEIKDSSSEEDAKSPNIFERAKEEIEAVIHSNKSPR----HHKETHGRSNDIDD 97
           +  + K E K    E+  K PN+ ++A+E+++++    K PR    HHKETHGRS+DI +
Sbjct: 52  KSSKDKMEFKSDKPEDGKKKPNMLKKAREDLKSLFLKEKPPRHLHHHHKETHGRSDDISE 111

Query: 98  TTPADEVKAPGVFERVKEEVEALVGAIHPKK 125
            TP DEVKAP V ER KEE+EA++  IH KK
Sbjct: 112 NTPVDEVKAPNVLERAKEEIEAVIDTIHSKK 142

BLAST of ClCG09G000780 vs. TAIR 10
Match: AT5G57000.2 (unknown protein; FUNCTIONS IN: molecular_function unknown; INVOLVED IN: biological_process unknown; LOCATED IN: cellular_component unknown; EXPRESSED IN: 24 plant structures; EXPRESSED DURING: 15 growth stages; BEST Arabidopsis thaliana protein match is: unknown protein (TAIR:AT1G72690.1); Has 141 Blast hits to 104 proteins in 24 species: Archae - 0; Bacteria - 6; Metazoa - 27; Fungi - 8; Plants - 82; Viruses - 0; Other Eukaryotes - 18 (source: NCBI BLink). )

HSP 1 Score: 80.5 bits (197), Expect = 1.1e-15
Identity = 43/91 (47.25%), Postives = 60/91 (65.93%), Query Frame = 0

Query: 38  RMEEPKAEIKDSSSEEDAKSPNIFERAKEEIEAVIHSNKSPR----HHKETHGRSNDIDD 97
           +  + K E K S   +  K PN+ ++A+E+++++    K PR    HHKETHGRS+DI +
Sbjct: 52  KSSKDKMEFK-SDKPDGKKKPNMLKKAREDLKSLFLKEKPPRHLHHHHKETHGRSDDISE 111

Query: 98  TTPADEVKAPGVFERVKEEVEALVGAIHPKK 125
            TP DEVKAP V ER KEE+EA++  IH KK
Sbjct: 112 NTPVDEVKAPNVLERAKEEIEAVIDTIHSKK 141

BLAST of ClCG09G000780 vs. TAIR 10
Match: AT1G17490.1 (unknown protein; BEST Arabidopsis thaliana protein match is: unknown protein (TAIR:AT1G72690.1); Has 57 Blast hits to 45 proteins in 9 species: Archae - 0; Bacteria - 0; Metazoa - 0; Fungi - 0; Plants - 57; Viruses - 0; Other Eukaryotes - 0 (source: NCBI BLink). )

HSP 1 Score: 60.8 bits (146), Expect = 9.0e-10
Identity = 39/83 (46.99%), Postives = 48/83 (57.83%), Query Frame = 0

Query: 44  AEIKDSSSEEDAKSPNIFERAKEEIEAVIHSNKSPRHHKETHGRSNDIDDTTPADEVKAP 103
           AE K SS  E     N+    KEE E++ H        KETHGRS DID+ T  D+VK P
Sbjct: 2   AEPKQSSLSE--VKTNVIGSRKEEKESMFHG-------KETHGRSEDIDEKTRVDDVKGP 61

Query: 104 GVFERVKEEVEALVGAIHPKKDS 127
           GV  R+ EE+EA+V A+ P K S
Sbjct: 62  GVLGRMMEEMEAIVDAVTPNKSS 75

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
XP_038900254.11.6e-3792.31uncharacterized protein LOC120087339 [Benincasa hispida] >XP_038900255.1 unchara... [more]
XP_008462344.12.6e-3589.01PREDICTED: uncharacterized protein LOC103500717 [Cucumis melo] >KAA0059422.1 unc... [more]
KGN45517.12.2e-3487.91hypothetical protein Csa_016837 [Cucumis sativus][more]
XP_022144348.12.9e-3483.52uncharacterized protein LOC111014044 isoform X2 [Momordica charantia][more]
XP_022144340.11.1e-3383.70uncharacterized protein LOC111014044 isoform X1 [Momordica charantia][more]
Match NameE-valueIdentityDescription
Match NameE-valueIdentityDescription
A0A5D3BY121.3e-3589.01Uncharacterized protein OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold... [more]
A0A1S3CGT31.3e-3589.01uncharacterized protein LOC103500717 OS=Cucumis melo OX=3656 GN=LOC103500717 PE=... [more]
A0A0A0KAY91.1e-3487.91Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_7G450740 PE=4 SV=1[more]
A0A6J1CT171.4e-3483.52uncharacterized protein LOC111014044 isoform X2 OS=Momordica charantia OX=3673 G... [more]
A0A6J1CRC85.3e-3483.70uncharacterized protein LOC111014044 isoform X1 OS=Momordica charantia OX=3673 G... [more]
Match NameE-valueIdentityDescription
AT1G72690.11.4e-1858.54unknown protein; BEST Arabidopsis thaliana protein match is: unknown protein (TA... [more]
AT5G57000.13.4e-1747.25unknown protein; FUNCTIONS IN: molecular_function unknown; INVOLVED IN: biologic... [more]
AT5G57000.21.1e-1547.25unknown protein; FUNCTIONS IN: molecular_function unknown; INVOLVED IN: biologic... [more]
AT1G17490.19.0e-1046.99unknown protein; BEST Arabidopsis thaliana protein match is: unknown protein (TA... [more]
InterPro
Analysis Name: InterPro Annotations of Watermelon (Charleston Gray) v2.5
Date Performed: 2022-01-31
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
NoneNo IPR availableMOBIDB_LITEmobidb-litedisorder_predictioncoord: 72..104
NoneNo IPR availableMOBIDB_LITEmobidb-litedisorder_predictioncoord: 13..59
NoneNo IPR availableMOBIDB_LITEmobidb-litedisorder_predictioncoord: 1..59
NoneNo IPR availablePANTHERPTHR35277:SF12PROTEIN, PUTATIVE-RELATEDcoord: 41..127
NoneNo IPR availablePANTHERPTHR35277OS09G0363700 PROTEINcoord: 41..127

Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
ClCG09G000780.2ClCG09G000780.2mRNA