ClCG08G010370 (gene) Watermelon (Charleston Gray) v2.5

Overview
NameClCG08G010370
Typegene
OrganismCitrullus lanatus subsp. vulgaris cv. Charleston Gray (Watermelon (Charleston Gray) v2.5)
DescriptionAB hydrolase-1 domain-containing protein
LocationCG_Chr08: 23093519 .. 23096824 (+)
RNA-Seq ExpressionClCG08G010370
SyntenyClCG08G010370
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: exonCDSpolypeptidethree_prime_UTR
Hold the cursor over a type above to highlight its positions in the sequence below.
ATGCTCTCTCTAACAATGGCTGCGAGTTGGGGCTCGGCTCCATTGCACATACAATGGAAGAAACCCAAGCGTCTGGGTGTCACCGTTAACGAATTTCCTTCATTCCTTCCCAAAGAAGTCCACAATATCAAAGACCAATTTGCTCGAAACCTTGCTACAAGGATTGAGAGGCTACCCGTAAGTTCAAACTTCCTACTTCTCTGGGAAAATTCATACTATTTCATCGTTTTTCAGTTGTTGGAACAATCATCTAATGATTATTTCACATGGGCTGGAGAAAATTGTAATGGAAACTCTCAATTCGAAGAAGTTCAAGCTATGTTACTTGCAGAATTCTGCCTGTCAGTGTGAATTTTGAATCGAAGGGGTCTTAAATTCTGCTAAACGTTCTGGGATTTAGCAGTGTTCCTTATCTTGCTTTGCATGTAGGTCAGCTTCTCCGATTCTTGTATTATGAGTAGTTGTGTGAAGCCGTCAATACAGAGTGAAGAACGTCCAGTGGTTCTTCTCCATGGCTTTGACAGGTTTCTTTCACTAATAAGAAACAATATTCGTGTTAGTTCTGTATTATTGTTATTATTTTATCTTTTCCTCGTTCATCACATCTTATTATTCAGCTCCTGTTTAGAGTGGAGATACACATATCCATTGCTTGAGGAAGCTGGTTTAGAAACTTGGGCTGTTGATATTCTTGGCTGGGGCTTCTCTGATTTAGGTTTGTCTAATTGCTTCATTCCTCATTTAATTGATCGAATACCCATTATTTCTATCTCTAATTTTCGTAGTTGCATGTGATAGAAAGACTCCCACCATGTGATGTGACTTCAAAACGTGTTCATTTGTATCAGGTAGTGAAAACCTCCTGTTTAGGTTGTTTTCCTGTTATGTATTTGGGGCAAATTTCTCTTCAATTCCAATTCTGTTCCCTTCCAATATGATCATCAAATGCTGACACTTGCATCATTGTTATAAGCTTGTTTTATTTTGTCTTCATTGCTATAACGTTTTCATTATTGTCCTGTTCTTTTTAATTCTTTTTTATATTCTGCATCGTTTCTGATTGTCAATCACACTTATATCTGTCCTTTTATGTTGAAACTAATAAAGTTTTTTCCTCATCTTGACAATAGCTTTGGAAATCATATATTAAAAGGCCGATGGTATTGGTTGGACCAAGCCTTGGTGCTGCTGTTGCAATTGATTTTGCTGTCAATTATCCAGAAGCTGTATGTATCAATTCGCTGAAATCTGTGACTACAAATTTGAAGTTATATCCTCACGTGAAATTATTGCAGAGAGTTATTCTCGAATGTAACAAGATTATTTCAGGTTGATAGGCTTGTGTTGATTGATGCAAGTGTATATGCAGAAGGTACAGGAAACTTAGCTACCCTACCAAGATTGCTTGCCTACGCTGGGGTGATTTGCTGCTTCTGTGAACATTCCATCAAGCATGCTAATGCTAAGCATTTTTAATGAGAGTTTGATTTTATGCAATTACAATTTACAAAACCACAAAATTTCCTTCATCTGTACGTCCTTTTCTATCCATGGAAGATACCATTATGGCATCACTACAGGCTTTTCATGATAATTCTTCAGTTAAAATCTTCTTGGAGAGAATCATTCCTCTAAATCTCTATCAACAAATTTGATTTTTTTTTTTTTTGCAAAGCAATTGGCAGTTTTGAGCGGGAAAGATTTTTTACATTTATTTCTTTTCTACAAAAATCATCTTGGAGATAATTGGTTATGTTTGTTGGTTTGTATGTGATGTGATTCGTAATTTTGTTAACTTATTTTGATCAGGTATTTTTATTGAAGAGCATTCCTTTGCGCTTATATGTGAACGTTTTGACCTTCACTGGCATATCATTTAGTACCGGCTTGGATTGGGCCAATGTAAGTTTTCCCCTACGTTAAGGAGGATGTTTATTATAGTTGTTGACTTGACGTGTATTTTGTGAAATTGCAAGTTTTATTTGACATGAAGATAAAATGCTGTGTCCATAACTAATTGACTCACCTCCTTCTCCGACTGTTCTCCTTCTATCCCTACTCACATTTTATTAGATCGGCCGCCTGCATTGTTTATTACCTTGGTGGGAGAATGCGACTGTTAGTTTTATGTTAAGTGGGGGATATAACGTCAGCAGCCAGATCAAAAAGGTTAGCACAATTCTTTTTGCTATTATATGGTTGTTGGAAAATGCAATGTTCTAAGTCACGATGTATCTTGTTCTTGGAACTAGAAAATTGCAAATATGTTAGTGAGACATAAGAAAAAGTTTCCATTCCTCATCAAGTTATGTAGGAATTTTCATCAAGGGTTGTTGAAATGAATTTTCTTTAAGGTCTTTTCATATTGTTCATTTGTCAATTATCATATCGACATTTGAGAATCTTTAGTTACTTCTGAATTCTGATAATTACGTGCATCTTGATTTAATAGGTGAAGCAGAAAACGCTTATTATATGGGGTGAGGATGATCAAATCATCAGCAACAAGCTAGGAGTGGTTAGTGTTTATTGCAATTCTGAAAAATGAGATTCAGATAGAAATTGAATTGGGTTGACAGAAAAGACTGACAAACGCCTTGAAACTAAGTAAATTGAAAAATAATGCTTGAACGTGAATGACTTTGTTGTCTTTTGTCTTTTGCATGGAATCATATATAACTATTGTTGCAAATAGAATAACTTCTCATTTGTCGAAATATGCAATGCAGAGGTTGCACTGTGAACTGCCAAATGCAGTTATTCGTCAAATACCCGAATGCGGGCATCTTCCTCATGTTGAAAAGCCAAATCCTGTTGCCAAATTGATTACACAATTTGTTCATGAAGATTCCAGAAAAGAGGTTCAGTATGTTAATAGTGGATAATTTTTCTTTGAACAAACTTACTATTTTCTTCATCTTATTAACGAATGTGCTTCATTCTGCTCATGAGTTCCCAATCCTGGCGTGTGTTCTGCAAAGAGGGCTGGAAGGTGCTCTACATGTTTCAAATTCAGTAAAATATAACATCATCAAAATAAACCCCCAAAAAATAAAAAGCAAGTCCATAATATCTGCTCCTGATTGTTGTTTTCTTTGCCTGATGATGGTGTAAATAATCCCCTCCTTCAAACATTGTAGGCTAGTTGGTGTAAATAATCCACTAGTGAAGTGGGGCAAGTTTTAAAAATGGAAAGGAATGATACCTACTGTCTAGAATATTAGAATCTCATCACATAACAACATAAAGTTGCAAGGTTAAGTAAAATGAGTTCTTGTAAGTTGGTTAGGAGTCTGTAAAATTAAA

mRNA sequence

ATGCTCTCTCTAACAATGGCTGCGAGTTGGGGCTCGGCTCCATTGCACATACAATGGAAGAAACCCAAGCGTCTGGGTGTCACCGTTAACGAATTTCCTTCATTCCTTCCCAAAGAAGTCCACAATATCAAAGACCAATTTGCTCGAAACCTTGCTACAAGGATTGAGAGGCTACCCGTAAGTTCAAACTTCCTACTTCTCTGGGAAAATTCATACTATTTCATCGTTTTTCAGTTGTTGGAACAATCATCTAATGATTATTTCACATGGGCTGGAGAAAATTGTAATGGAAACTCTCAATTCGAAGAAGTTCAAGCTATGTTACTTGCAGAATTCTGCCTGTCAGTCAGCTTCTCCGATTCTTGTATTATGAGTAGTTGTGTGAAGCCGTCAATACAGAGTGAAGAACGTCCAGTGGTTCTTCTCCATGGCTTTGACAGCTCCTGTTTAGAGTGGAGATACACATATCCATTGCTTGAGGAAGCTGGTTTAGAAACTTGGGCTGTTGATATTCTTGGCTGGGGCTTCTCTGATTTAGAAAGACTCCCACCATGTGATGTGACTTCAAAACGTGTTCATTTGTATCAGCTTTGGAAATCATATATTAAAAGGCCGATGGTATTGGTTGGACCAAGCCTTGGTGCTGCTGTTGCAATTGATTTTGCTGTCAATTATCCAGAAGCTGTTGATAGGCTTGTGTTGATTGATGCAAGTGTATATGCAGAAGGTACAGGAAACTTAGCTACCCTACCAAGATTGCTTGCCTACGCTGGGGTATTTTTATTGAAGAGCATTCCTTTGCGCTTATATGTGAACGTTTTGACCTTCACTGGCATATCATTTAGTACCGGCTTGGATTGGGCCAATATCGGCCGCCTGCATTGTTTATTACCTTGGTGGGAGAATGCGACTGTTAGTTTTATGTTAAGTGGGGGATATAACGTCAGCAGCCAGATCAAAAAGGTGAAGCAGAAAACGCTTATTATATGGGGTGAGGATGATCAAATCATCAGCAACAAGCTAGGAGTGAGGTTGCACTGTGAACTGCCAAATGCAGTTATTCGTCAAATACCCGAATGCGGGCATCTTCCTCATGTTGAAAAGCCAAATCCTGTTGCCAAATTGATTACACAATTTGTTCATGAAGATTCCAGAAAAGAGGTTCAGTATGTTAATAGTGGATAATTTTTCTTTGAACAAACTTACTATTTTCTTCATCTTATTAACGAATGTGCTTCATTCTGCTCATGAGTTCCCAATCCTGGCGTGTGTTCTGCAAAGAGGGCTGGAAGGTGCTCTACATGTTTCAAATTCAGTAAAATATAACATCATCAAAATAAACCCCCAAAAAATAAAAAGCAAGTCCATAATATCTGCTCCTGATTGTTGTTTTCTTTGCCTGATGATGGTGTAAATAATCCCCTCCTTCAAACATTGTAGGCTAGTTGGTGTAAATAATCCACTAGTGAAGTGGGGCAAGTTTTAAAAATGGAAAGGAATGATACCTACTGTCTAGAATATTAGAATCTCATCACATAACAACATAAAGTTGCAAGGTTAAGTAAAATGAGTTCTTGTAAGTTGGTTAGGAGTCTGTAAAATTAAA

Coding sequence (CDS)

ATGCTCTCTCTAACAATGGCTGCGAGTTGGGGCTCGGCTCCATTGCACATACAATGGAAGAAACCCAAGCGTCTGGGTGTCACCGTTAACGAATTTCCTTCATTCCTTCCCAAAGAAGTCCACAATATCAAAGACCAATTTGCTCGAAACCTTGCTACAAGGATTGAGAGGCTACCCGTAAGTTCAAACTTCCTACTTCTCTGGGAAAATTCATACTATTTCATCGTTTTTCAGTTGTTGGAACAATCATCTAATGATTATTTCACATGGGCTGGAGAAAATTGTAATGGAAACTCTCAATTCGAAGAAGTTCAAGCTATGTTACTTGCAGAATTCTGCCTGTCAGTCAGCTTCTCCGATTCTTGTATTATGAGTAGTTGTGTGAAGCCGTCAATACAGAGTGAAGAACGTCCAGTGGTTCTTCTCCATGGCTTTGACAGCTCCTGTTTAGAGTGGAGATACACATATCCATTGCTTGAGGAAGCTGGTTTAGAAACTTGGGCTGTTGATATTCTTGGCTGGGGCTTCTCTGATTTAGAAAGACTCCCACCATGTGATGTGACTTCAAAACGTGTTCATTTGTATCAGCTTTGGAAATCATATATTAAAAGGCCGATGGTATTGGTTGGACCAAGCCTTGGTGCTGCTGTTGCAATTGATTTTGCTGTCAATTATCCAGAAGCTGTTGATAGGCTTGTGTTGATTGATGCAAGTGTATATGCAGAAGGTACAGGAAACTTAGCTACCCTACCAAGATTGCTTGCCTACGCTGGGGTATTTTTATTGAAGAGCATTCCTTTGCGCTTATATGTGAACGTTTTGACCTTCACTGGCATATCATTTAGTACCGGCTTGGATTGGGCCAATATCGGCCGCCTGCATTGTTTATTACCTTGGTGGGAGAATGCGACTGTTAGTTTTATGTTAAGTGGGGGATATAACGTCAGCAGCCAGATCAAAAAGGTGAAGCAGAAAACGCTTATTATATGGGGTGAGGATGATCAAATCATCAGCAACAAGCTAGGAGTGAGGTTGCACTGTGAACTGCCAAATGCAGTTATTCGTCAAATACCCGAATGCGGGCATCTTCCTCATGTTGAAAAGCCAAATCCTGTTGCCAAATTGATTACACAATTTGTTCATGAAGATTCCAGAAAAGAGGTTCAGTATGTTAATAGTGGATAA

Protein sequence

MLSLTMAASWGSAPLHIQWKKPKRLGVTVNEFPSFLPKEVHNIKDQFARNLATRIERLPVSSNFLLLWENSYYFIVFQLLEQSSNDYFTWAGENCNGNSQFEEVQAMLLAEFCLSVSFSDSCIMSSCVKPSIQSEERPVVLLHGFDSSCLEWRYTYPLLEEAGLETWAVDILGWGFSDLERLPPCDVTSKRVHLYQLWKSYIKRPMVLVGPSLGAAVAIDFAVNYPEAVDRLVLIDASVYAEGTGNLATLPRLLAYAGVFLLKSIPLRLYVNVLTFTGISFSTGLDWANIGRLHCLLPWWENATVSFMLSGGYNVSSQIKKVKQKTLIIWGEDDQIISNKLGVRLHCELPNAVIRQIPECGHLPHVEKPNPVAKLITQFVHEDSRKEVQYVNSG
Homology
BLAST of ClCG08G010370 vs. NCBI nr
Match: XP_038884275.1 (uncharacterized hydrolase YugF isoform X2 [Benincasa hispida])

HSP 1 Score: 640.2 bits (1650), Expect = 1.2e-179
Identity = 321/393 (81.68%), Postives = 330/393 (83.97%), Query Frame = 0

Query: 1   MLSLTMAASWGSAPLHIQWKKPKRLGVTVNEFPSFLPKEVHNIKDQFARNLATRIERLPV 60
           MLSLTMAASWGSAPLHIQWKK K L VT+NEFPSFLPKEVHNIKDQFARNLATRIERLP 
Sbjct: 1   MLSLTMAASWGSAPLHIQWKKAKHLCVTINEFPSFLPKEVHNIKDQFARNLATRIERLP- 60

Query: 61  SSNFLLLWENSYYFIVFQLLEQSSNDYFTWAGENCNGNSQFEEVQAMLLAEFCLSVSFSD 120
                                                                  VSFSD
Sbjct: 61  -------------------------------------------------------VSFSD 120

Query: 121 SCIMSSCVKPSIQSEERPVVLLHGFDSSCLEWRYTYPLLEEAGLETWAVDILGWGFSDLE 180
           SCIMSSCVKPSIQS+ERPVVLLHGFDSSCLEWRYTYPLLEEAGLETWAVDILGWGFSDLE
Sbjct: 121 SCIMSSCVKPSIQSKERPVVLLHGFDSSCLEWRYTYPLLEEAGLETWAVDILGWGFSDLE 180

Query: 181 RLPPCDVTSKRVHLYQLWKSYIKRPMVLVGPSLGAAVAIDFAVNYPEAVDRLVLIDASVY 240
           RLPPCDVTSKRVHLYQLWKSYIKRPMVLVGPSLGAAVAIDFAVNYP+AVDRLVLIDASVY
Sbjct: 181 RLPPCDVTSKRVHLYQLWKSYIKRPMVLVGPSLGAAVAIDFAVNYPKAVDRLVLIDASVY 240

Query: 241 AEGTGNLATLPRLLAYAGVFLLKSIPLRLYVNVLTFTGISFSTGLDWANIGRLHCLLPWW 300
           AEGTGNLATLPRL+AYAGVFLLKSIPLRLYVNVLTF GISFST LDWANIGRLHCLLPWW
Sbjct: 241 AEGTGNLATLPRLIAYAGVFLLKSIPLRLYVNVLTFAGISFSTSLDWANIGRLHCLLPWW 300

Query: 301 ENATVSFMLSGGYNVSSQIKKVKQKTLIIWGEDDQIISNKLGVRLHCELPNAVIRQIPEC 360
           E+ATVSFMLSGGYNVSSQI+KVKQK+LIIWGEDDQIIS+KLGVRLHCELPNA+IRQIP C
Sbjct: 301 EDATVSFMLSGGYNVSSQIEKVKQKSLIIWGEDDQIISSKLGVRLHCELPNAIIRQIPGC 337

Query: 361 GHLPHVEKPNPVAKLITQFVHEDSRKEVQYVNS 394
           GHLPHVEKPN VAKLITQFVHEDSRKEVQYVNS
Sbjct: 361 GHLPHVEKPNLVAKLITQFVHEDSRKEVQYVNS 337

BLAST of ClCG08G010370 vs. NCBI nr
Match: XP_038884274.1 (uncharacterized hydrolase YugF isoform X1 [Benincasa hispida])

HSP 1 Score: 629.4 bits (1622), Expect = 2.1e-176
Identity = 321/410 (78.29%), Postives = 330/410 (80.49%), Query Frame = 0

Query: 1   MLSLTMAASWGSAPLHIQWKKPKRLGVTVNEFPSFLPKEVHNIKDQFARNLATRIERLPV 60
           MLSLTMAASWGSAPLHIQWKK K L VT+NEFPSFLPKEVHNIKDQFARNLATRIERLP 
Sbjct: 1   MLSLTMAASWGSAPLHIQWKKAKHLCVTINEFPSFLPKEVHNIKDQFARNLATRIERLP- 60

Query: 61  SSNFLLLWENSYYFIVFQLLEQSSNDYFTWAGENCNGNSQFEEVQAMLLAEFCLSVSFSD 120
                                                                  VSFSD
Sbjct: 61  -------------------------------------------------------VSFSD 120

Query: 121 SCIMSSCVKPSIQSEERPVVLLHGFDSSCLEWRYTYPLLEEAGLETWAVDILGWGFSDLE 180
           SCIMSSCVKPSIQS+ERPVVLLHGFDSSCLEWRYTYPLLEEAGLETWAVDILGWGFSDLE
Sbjct: 121 SCIMSSCVKPSIQSKERPVVLLHGFDSSCLEWRYTYPLLEEAGLETWAVDILGWGFSDLE 180

Query: 181 RLPPCDVTSKRVHLYQ-----------------LWKSYIKRPMVLVGPSLGAAVAIDFAV 240
           RLPPCDVTSKRVHLYQ                 LWKSYIKRPMVLVGPSLGAAVAIDFAV
Sbjct: 181 RLPPCDVTSKRVHLYQVMKTCSYVFGEIFSSIPLWKSYIKRPMVLVGPSLGAAVAIDFAV 240

Query: 241 NYPEAVDRLVLIDASVYAEGTGNLATLPRLLAYAGVFLLKSIPLRLYVNVLTFTGISFST 300
           NYP+AVDRLVLIDASVYAEGTGNLATLPRL+AYAGVFLLKSIPLRLYVNVLTF GISFST
Sbjct: 241 NYPKAVDRLVLIDASVYAEGTGNLATLPRLIAYAGVFLLKSIPLRLYVNVLTFAGISFST 300

Query: 301 GLDWANIGRLHCLLPWWENATVSFMLSGGYNVSSQIKKVKQKTLIIWGEDDQIISNKLGV 360
            LDWANIGRLHCLLPWWE+ATVSFMLSGGYNVSSQI+KVKQK+LIIWGEDDQIIS+KLGV
Sbjct: 301 SLDWANIGRLHCLLPWWEDATVSFMLSGGYNVSSQIEKVKQKSLIIWGEDDQIISSKLGV 354

Query: 361 RLHCELPNAVIRQIPECGHLPHVEKPNPVAKLITQFVHEDSRKEVQYVNS 394
           RLHCELPNA+IRQIP CGHLPHVEKPN VAKLITQFVHEDSRKEVQYVNS
Sbjct: 361 RLHCELPNAIIRQIPGCGHLPHVEKPNLVAKLITQFVHEDSRKEVQYVNS 354

BLAST of ClCG08G010370 vs. NCBI nr
Match: KAA0064761.1 (putative hydrolase YugF [Cucumis melo var. makuwa])

HSP 1 Score: 617.8 bits (1592), Expect = 6.3e-173
Identity = 308/393 (78.37%), Postives = 324/393 (82.44%), Query Frame = 0

Query: 1   MLSLTMAASWGSAPLHIQWKKPKRLGVTVNEFPSFLPKEVHNIKDQFARNLATRIERLPV 60
           MLS TMAA+WGSAPLHIQWKKPKR+GVTVNEFPSFLPKEV++IKDQFARNLA+RIERLP 
Sbjct: 1   MLSPTMAATWGSAPLHIQWKKPKRMGVTVNEFPSFLPKEVYDIKDQFARNLASRIERLP- 60

Query: 61  SSNFLLLWENSYYFIVFQLLEQSSNDYFTWAGENCNGNSQFEEVQAMLLAEFCLSVSFSD 120
                                                                  VSFSD
Sbjct: 61  -------------------------------------------------------VSFSD 120

Query: 121 SCIMSSCVKPSIQSEERPVVLLHGFDSSCLEWRYTYPLLEEAGLETWAVDILGWGFSDLE 180
           SCIMSSCVKPSIQS++ PVVLLHGFDSSCLEWRYTYPLLEEAGLETWAVD+LGWGFSDL 
Sbjct: 121 SCIMSSCVKPSIQSKKCPVVLLHGFDSSCLEWRYTYPLLEEAGLETWAVDVLGWGFSDLG 180

Query: 181 RLPPCDVTSKRVHLYQLWKSYIKRPMVLVGPSLGAAVAIDFAVNYPEAVDRLVLIDASVY 240
            LPPCDVTSKRVHLYQLWKSYIKRPMV+VGPSLGAAVAIDFAVNYPEAVDRLVLIDASVY
Sbjct: 181 LLPPCDVTSKRVHLYQLWKSYIKRPMVIVGPSLGAAVAIDFAVNYPEAVDRLVLIDASVY 240

Query: 241 AEGTGNLATLPRLLAYAGVFLLKSIPLRLYVNVLTFTGISFSTGLDWANIGRLHCLLPWW 300
           AEGTGNLATLPRL+AYAGVFLLKSIPLR+YVN LTFT I FSTGLDWANIGRLHCLLPWW
Sbjct: 241 AEGTGNLATLPRLIAYAGVFLLKSIPLRVYVNFLTFTSIPFSTGLDWANIGRLHCLLPWW 300

Query: 301 ENATVSFMLSGGYNVSSQIKKVKQKTLIIWGEDDQIISNKLGVRLHCELPNAVIRQIPEC 360
           E+ATVSFMLSGGY VSSQI+KVKQKTLIIWGEDDQI+S KLGVRLHCELPNAV R I EC
Sbjct: 301 EDATVSFMLSGGYKVSSQIEKVKQKTLIIWGEDDQIVSYKLGVRLHCELPNAVFRPIAEC 337

Query: 361 GHLPHVEKPNPVAKLITQFVHEDSRKEVQYVNS 394
           GHLPHVEKPN VAKLITQF+HEDSRKEVQ+VNS
Sbjct: 361 GHLPHVEKPNLVAKLITQFIHEDSRKEVQFVNS 337

BLAST of ClCG08G010370 vs. NCBI nr
Match: XP_008445456.1 (PREDICTED: uncharacterized hydrolase YugF [Cucumis melo])

HSP 1 Score: 613.2 bits (1580), Expect = 1.5e-171
Identity = 305/387 (78.81%), Postives = 320/387 (82.69%), Query Frame = 0

Query: 1   MLSLTMAASWGSAPLHIQWKKPKRLGVTVNEFPSFLPKEVHNIKDQFARNLATRIERLPV 60
           MLS TMAA+WGSAPLHIQWKKPKR+GVTVNEFPSFLPKEV++IKDQFARNLA+RIERLP 
Sbjct: 1   MLSPTMAATWGSAPLHIQWKKPKRMGVTVNEFPSFLPKEVYDIKDQFARNLASRIERLP- 60

Query: 61  SSNFLLLWENSYYFIVFQLLEQSSNDYFTWAGENCNGNSQFEEVQAMLLAEFCLSVSFSD 120
                                                                  VSFSD
Sbjct: 61  -------------------------------------------------------VSFSD 120

Query: 121 SCIMSSCVKPSIQSEERPVVLLHGFDSSCLEWRYTYPLLEEAGLETWAVDILGWGFSDLE 180
           SCIMSSCVKPSIQS++ PVVLLHGFDSSCLEWRYTYPLLEEAGLETWAVD+LGWGFSDLE
Sbjct: 121 SCIMSSCVKPSIQSKKCPVVLLHGFDSSCLEWRYTYPLLEEAGLETWAVDVLGWGFSDLE 180

Query: 181 RLPPCDVTSKRVHLYQLWKSYIKRPMVLVGPSLGAAVAIDFAVNYPEAVDRLVLIDASVY 240
           RLPPCDVTSKRVHLYQLWKSYIKRPMV+VGPSLGAAVAIDFAVNYPEAVDRLVLIDASVY
Sbjct: 181 RLPPCDVTSKRVHLYQLWKSYIKRPMVIVGPSLGAAVAIDFAVNYPEAVDRLVLIDASVY 240

Query: 241 AEGTGNLATLPRLLAYAGVFLLKSIPLRLYVNVLTFTGISFSTGLDWANIGRLHCLLPWW 300
           AEGTGNLATLPRL+AYAGVFLLKSIPLR+YVN LTFT I FSTGLDWANIGRLHCLLPWW
Sbjct: 241 AEGTGNLATLPRLIAYAGVFLLKSIPLRVYVNFLTFTSIPFSTGLDWANIGRLHCLLPWW 300

Query: 301 ENATVSFMLSGGYNVSSQIKKVKQKTLIIWGEDDQIISNKLGVRLHCELPNAVIRQIPEC 360
           E+ATVSFMLSGGY VSSQI+KVKQKTLIIWGEDDQI+S KLGVRLHCELPNAV R I EC
Sbjct: 301 EDATVSFMLSGGYKVSSQIEKVKQKTLIIWGEDDQIVSYKLGVRLHCELPNAVFRPIAEC 331

Query: 361 GHLPHVEKPNPVAKLITQFVHEDSRKE 388
           GHLPHVEKPN VAKLITQF+HEDSRKE
Sbjct: 361 GHLPHVEKPNLVAKLITQFIHEDSRKE 331

BLAST of ClCG08G010370 vs. NCBI nr
Match: KAG7029571.1 (yugF, partial [Cucurbita argyrosperma subsp. argyrosperma])

HSP 1 Score: 612.8 bits (1579), Expect = 2.0e-171
Identity = 310/393 (78.88%), Postives = 319/393 (81.17%), Query Frame = 0

Query: 1   MLSLTMAASWGSAPLHIQWKKPKRLGVTVNEFPSFLPKEVHNIKDQFARNLATRIERLPV 60
           MLSL +AASW SAP  +QWKKPKRLGV +N+FPSFLPKEV NIKDQFAR LATRIERLP 
Sbjct: 27  MLSLAVAASWCSAPPLMQWKKPKRLGVILNKFPSFLPKEVDNIKDQFARELATRIERLP- 86

Query: 61  SSNFLLLWENSYYFIVFQLLEQSSNDYFTWAGENCNGNSQFEEVQAMLLAEFCLSVSFSD 120
                                                                  VSFSD
Sbjct: 87  -------------------------------------------------------VSFSD 146

Query: 121 SCIMSSCVKPSIQSEERPVVLLHGFDSSCLEWRYTYPLLEEAGLETWAVDILGWGFSDLE 180
           SC MSSCVKPSIQS+E PVVLLHGFDSSCLEWRYTYPLLEEAG ETWAVDILGWGFSDLE
Sbjct: 147 SCTMSSCVKPSIQSKECPVVLLHGFDSSCLEWRYTYPLLEEAGFETWAVDILGWGFSDLE 206

Query: 181 RLPPCDVTSKRVHLYQLWKSYIKRPMVLVGPSLGAAVAIDFAVNYPEAVDRLVLIDASVY 240
           RLPPCDVTSKRVHLYQLWKSYIKRPMVLVGPSLGAAVAIDFAVNYPEAVDRLVLIDASVY
Sbjct: 207 RLPPCDVTSKRVHLYQLWKSYIKRPMVLVGPSLGAAVAIDFAVNYPEAVDRLVLIDASVY 266

Query: 241 AEGTGNLATLPRLLAYAGVFLLKSIPLRLYVNVLTFTGISFSTGLDWANIGRLHCLLPWW 300
           AEGTGNLATLPRL+AYAGVFLLKSIPLRLYVNVL FTGIS ST LDW NIGRLHCLLPWW
Sbjct: 267 AEGTGNLATLPRLIAYAGVFLLKSIPLRLYVNVLAFTGISLSTSLDWVNIGRLHCLLPWW 326

Query: 301 ENATVSFMLSGGYNVSSQIKKVKQKTLIIWGEDDQIISNKLGVRLHCELPNAVIRQIPEC 360
           E+ATVSFMLSGGYNVSSQI+KVKQKTLIIWGEDDQIISNKL VRLHCELPNA IRQIPEC
Sbjct: 327 EDATVSFMLSGGYNVSSQIEKVKQKTLIIWGEDDQIISNKLAVRLHCELPNAFIRQIPEC 363

Query: 361 GHLPHVEKPNPVAKLITQFVHEDSRKEVQYVNS 394
           GHLPHVEKPN VAKLITQFVHEDSRKEV YVN+
Sbjct: 387 GHLPHVEKPNFVAKLITQFVHEDSRKEVPYVNN 363

BLAST of ClCG08G010370 vs. ExPASy Swiss-Prot
Match: O05235 (Uncharacterized hydrolase YugF OS=Bacillus subtilis (strain 168) OX=224308 GN=yugF PE=3 SV=1)

HSP 1 Score: 86.7 bits (213), Expect = 6.6e-16
Identity = 64/246 (26.02%), Postives = 118/246 (47.97%), Query Frame = 0

Query: 139 VVLLHGFDSSCLEWRYTYPLLEEAGLETWAVDILGWGFSDLERLPPCDVTSKRVHLYQLW 198
           +V +HGF SS   +R   PLL +   +  A+D+  +G S+  R       +    +  + 
Sbjct: 30  LVCVHGFLSSAFSFRKVIPLLRDK-YDIIALDLPPFGQSEKSRTFIYTYQNLAKLVIGIL 89

Query: 199 KSYIKRPMVLVGPSLGAAVAIDFAVNYPEAVDRLVLIDASVYAEGTGNL----ATLPRLL 258
           +    +  VLVG S+G  +++  A+  PE   ++VL+ +S Y + +         +P   
Sbjct: 90  EHLQVKQAVLVGHSMGGQISLSAALQKPELFSKVVLLCSSGYLKRSHPTIIFGTHIPYFH 149

Query: 259 AYAGVFLLKSIPLRLYVNVLTFTGISFSTGLDWANIGRLHCLLPWWENATVSFMLSGGYN 318
            Y   +L K   ++  +NV+    +     +D    GR       ++  T       G  
Sbjct: 150 LYIKRWLSKEGVMKNLLNVVHDKSLIDEEMID--GYGRPFQDEQIFKAMTRFIRHREGDL 209

Query: 319 VSSQIKKVKQKTLIIWGEDDQIISNKLGVRLHCELPNAVIRQIPECGHLPHVEKPNPVAK 378
              Q+KK+ +  L+IWGE+D+I+  ++G RLH +LPN+V+  + + GHL   E+P  +++
Sbjct: 210 EPEQLKKMNKPALLIWGEEDRIVPMEIGKRLHADLPNSVLYSLGQTGHLVPEERPELISE 269

Query: 379 LITQFV 381
            I  F+
Sbjct: 270 HIADFI 272

BLAST of ClCG08G010370 vs. ExPASy Swiss-Prot
Match: Q476M7 (2-hydroxy-6-oxononadienedioate/2-hydroxy-6-oxononatrienedioate hydrolase OS=Cupriavidus pinatubonensis (strain JMP 134 / LMG 1197) OX=264198 GN=mhpC PE=3 SV=1)

HSP 1 Score: 76.6 bits (187), Expect = 6.8e-13
Identity = 72/260 (27.69%), Postives = 112/260 (43.08%), Query Frame = 0

Query: 139 VVLLHGFDSSCLEW----RYTYPLLEEAGLETWAVDILGWGFSDLERLPPCDVTSK---- 198
           VV+LHG       W    R   PL+  AG     VD  GWG SD    P  +  S+    
Sbjct: 40  VVMLHGSGPGATGWANFNRNVEPLV-AAGYRVLLVDCPGWGKSD----PVVNAGSRSELN 99

Query: 199 -RVHLYQLWKSYIKRPMVLVGPSLGAAVAIDFAVNYPEAVDRLVLIDASVYAEGTGNLAT 258
            RV    L +  I+R  +L G S+G   A+ FA+  P+ V +LVL+       GTG   +
Sbjct: 100 GRVLKGVLDELDIERVHIL-GNSMGGHSAVAFALANPQRVGKLVLMGG-----GTGG-PS 159

Query: 259 LPRLLAYAGVFLLKSI-------PLRLYVNVLTFTGISFSTGLDWANIGRLHCLLPWWEN 318
           L   +   G+ LL  +        L+  +NV  +   S +  L  A +  +       EN
Sbjct: 160 LYAPMPTEGIKLLNGLYREPSIENLKRMMNVFVYDASSLTDDLMQARLDNMLARRDHLEN 219

Query: 319 ATVSFMLSGGY--NVSSQIKKVKQKTLIIWGEDDQIISNKLGVRLHCELPNAVIRQIPEC 378
              S   +     +  S++ ++   TL+IWG DD+ +   +G+RL   +PNA +     C
Sbjct: 220 FVKSLAANPKQFTDYGSRLGEITAPTLVIWGRDDRFVPMDVGLRLIAGIPNAQMHIFNRC 279

Query: 379 GHLPHVEKPNPVAKLITQFV 381
           GH    E      +++  F+
Sbjct: 280 GHWAQWEHAKAFNRMVVDFL 287

BLAST of ClCG08G010370 vs. ExPASy Swiss-Prot
Match: Q8KZP5 (2-hydroxy-6-oxononadienedioate/2-hydroxy-6-oxononatrienedioate hydrolase OS=Comamonas testosteroni OX=285 GN=mhpC PE=1 SV=1)

HSP 1 Score: 73.9 bits (180), Expect = 4.4e-12
Identity = 57/181 (31.49%), Postives = 89/181 (49.17%), Query Frame = 0

Query: 208 LVGPSLGAAVAIDFAVNYPEAVDRLVLIDASVYAEGTGN-LATLPRLLAYAGVFLLKSIP 267
           LVG S+G A A++FA+ YPE   +L+L+       G GN L T   +     +F L + P
Sbjct: 108 LVGNSMGGAGALNFALEYPERTGKLILMGPG----GLGNSLFTAMPMEGIKLLFKLYAEP 167

Query: 268 ----LRLYVNVLTFTGISFSTGL---DWANIGRLHCLLPWWENATVSFMLSGGYNVSSQI 327
               L+  +NV  F     +  L    WANI R    L  +  ++    LS  +NVS ++
Sbjct: 168 SLDTLKQMLNVFLFDQSLITDELVQGRWANIQRNPEHLKNFLLSSQKLPLS-SWNVSPRM 227

Query: 328 KKVKQKTLIIWGEDDQIISNKLGVRLHCELPNAVIRQIPECGHLPHVEKPNPVAKLITQF 381
            ++K KTL+ WG DD+ +    G++L   +P+A +   P CGH    E  +   +L   F
Sbjct: 228 GEIKAKTLVTWGRDDRFVPLDHGLKLVANMPDAQLHVFPRCGHWAQWEHADAFNRLTLDF 283

BLAST of ClCG08G010370 vs. ExPASy Swiss-Prot
Match: Q2VLB9 (2-hydroxy-6-oxo-6-phenylhexa-2,4-dienoate hydrolase OS=Burkholderia cepacia OX=292 GN=bphD PE=3 SV=1)

HSP 1 Score: 70.5 bits (171), Expect = 4.9e-11
Identity = 55/184 (29.89%), Postives = 89/184 (48.37%), Query Frame = 0

Query: 208 LVGPSLGAAVAIDFAVNYPEAVDRLVLIDASVYAEGTGNL-ATLPRLLAYAGVFLLKSI- 267
           LVG S+G A A++FA+ YPE   +++L+       G G L A+L   +   G+ LL  + 
Sbjct: 108 LVGNSMGGAGALNFALEYPERTGKVILM-------GPGGLGASLFNPMPMEGIKLLFKLY 167

Query: 268 ------PLRLYVNVLTFTGISFSTGL---DWANIGRLHCLLPWWENATVSFMLSGGYNVS 327
                  L+  +NV  F     +  L    WANI R    L  +  +     LS  ++VS
Sbjct: 168 AEPSLETLKQMLNVFMFDQSLITDELLQGRWANIQRNPEHLKNFILSAQKVPLS-AWDVS 227

Query: 328 SQIKKVKQKTLIIWGEDDQIISNKLGVRLHCELPNAVIRQIPECGHLPHVEKPNPVAKLI 381
            ++ ++K KTL+ WG DD+ +    G++L   +P+A +   P CGH    E  +   +L 
Sbjct: 228 PRLGEIKAKTLVTWGRDDRFVPLDHGLKLVANMPDAQLHVFPRCGHWAQWEHADAFNRLT 283

BLAST of ClCG08G010370 vs. ExPASy Swiss-Prot
Match: P17548 (2-hydroxy-6-oxo-6-phenylhexa-2,4-dienoate hydrolase OS=Pseudomonas sp. (strain KKS102) OX=307 GN=bphD PE=1 SV=2)

HSP 1 Score: 67.0 bits (162), Expect = 5.4e-10
Identity = 54/181 (29.83%), Postives = 87/181 (48.07%), Query Frame = 0

Query: 208 LVGPSLGAAVAIDFAVNYPEAVDRLVLIDASVYAEGTGN-LATLPRLLAYAGVFLLKSIP 267
           LVG S+G A A++FA+ YPE   +L+L+       G GN L T   +     +F L + P
Sbjct: 108 LVGNSMGGAGALNFALEYPERTGKLILMGPG----GLGNSLFTAMPMEGIKLLFKLYAEP 167

Query: 268 ----LRLYVNVLTFTGISFSTGL---DWANIGRLHCLLPWWENATVSFMLSGGYNVSSQI 327
               L+  +NV  F     +  L    WANI R    L  +  +     LS  ++VS+++
Sbjct: 168 SLETLKQMLNVFLFDQSVITDELLQGRWANIQRNPEHLKNFILSAQKVPLS-AWDVSARL 227

Query: 328 KKVKQKTLIIWGEDDQIISNKLGVRLHCELPNAVIRQIPECGHLPHVEKPNPVAKLITQF 381
            ++K KTL+ WG DD+ +    G++L   + +A +   P C H    E  +   +L   F
Sbjct: 228 GEIKAKTLVTWGRDDRFVPLDHGLKLIANMQDAHVHVFPRCAHWAQWEHADAFNRLTLDF 283

BLAST of ClCG08G010370 vs. ExPASy TrEMBL
Match: A0A5A7VGC5 (Putative hydrolase YugF OS=Cucumis melo var. makuwa OX=1194695 GN=E6C27_scaffold82G00660 PE=4 SV=1)

HSP 1 Score: 617.8 bits (1592), Expect = 3.0e-173
Identity = 308/393 (78.37%), Postives = 324/393 (82.44%), Query Frame = 0

Query: 1   MLSLTMAASWGSAPLHIQWKKPKRLGVTVNEFPSFLPKEVHNIKDQFARNLATRIERLPV 60
           MLS TMAA+WGSAPLHIQWKKPKR+GVTVNEFPSFLPKEV++IKDQFARNLA+RIERLP 
Sbjct: 1   MLSPTMAATWGSAPLHIQWKKPKRMGVTVNEFPSFLPKEVYDIKDQFARNLASRIERLP- 60

Query: 61  SSNFLLLWENSYYFIVFQLLEQSSNDYFTWAGENCNGNSQFEEVQAMLLAEFCLSVSFSD 120
                                                                  VSFSD
Sbjct: 61  -------------------------------------------------------VSFSD 120

Query: 121 SCIMSSCVKPSIQSEERPVVLLHGFDSSCLEWRYTYPLLEEAGLETWAVDILGWGFSDLE 180
           SCIMSSCVKPSIQS++ PVVLLHGFDSSCLEWRYTYPLLEEAGLETWAVD+LGWGFSDL 
Sbjct: 121 SCIMSSCVKPSIQSKKCPVVLLHGFDSSCLEWRYTYPLLEEAGLETWAVDVLGWGFSDLG 180

Query: 181 RLPPCDVTSKRVHLYQLWKSYIKRPMVLVGPSLGAAVAIDFAVNYPEAVDRLVLIDASVY 240
            LPPCDVTSKRVHLYQLWKSYIKRPMV+VGPSLGAAVAIDFAVNYPEAVDRLVLIDASVY
Sbjct: 181 LLPPCDVTSKRVHLYQLWKSYIKRPMVIVGPSLGAAVAIDFAVNYPEAVDRLVLIDASVY 240

Query: 241 AEGTGNLATLPRLLAYAGVFLLKSIPLRLYVNVLTFTGISFSTGLDWANIGRLHCLLPWW 300
           AEGTGNLATLPRL+AYAGVFLLKSIPLR+YVN LTFT I FSTGLDWANIGRLHCLLPWW
Sbjct: 241 AEGTGNLATLPRLIAYAGVFLLKSIPLRVYVNFLTFTSIPFSTGLDWANIGRLHCLLPWW 300

Query: 301 ENATVSFMLSGGYNVSSQIKKVKQKTLIIWGEDDQIISNKLGVRLHCELPNAVIRQIPEC 360
           E+ATVSFMLSGGY VSSQI+KVKQKTLIIWGEDDQI+S KLGVRLHCELPNAV R I EC
Sbjct: 301 EDATVSFMLSGGYKVSSQIEKVKQKTLIIWGEDDQIVSYKLGVRLHCELPNAVFRPIAEC 337

Query: 361 GHLPHVEKPNPVAKLITQFVHEDSRKEVQYVNS 394
           GHLPHVEKPN VAKLITQF+HEDSRKEVQ+VNS
Sbjct: 361 GHLPHVEKPNLVAKLITQFIHEDSRKEVQFVNS 337

BLAST of ClCG08G010370 vs. ExPASy TrEMBL
Match: A0A1S3BDL9 (uncharacterized hydrolase YugF OS=Cucumis melo OX=3656 GN=LOC103488473 PE=4 SV=1)

HSP 1 Score: 613.2 bits (1580), Expect = 7.5e-172
Identity = 305/387 (78.81%), Postives = 320/387 (82.69%), Query Frame = 0

Query: 1   MLSLTMAASWGSAPLHIQWKKPKRLGVTVNEFPSFLPKEVHNIKDQFARNLATRIERLPV 60
           MLS TMAA+WGSAPLHIQWKKPKR+GVTVNEFPSFLPKEV++IKDQFARNLA+RIERLP 
Sbjct: 1   MLSPTMAATWGSAPLHIQWKKPKRMGVTVNEFPSFLPKEVYDIKDQFARNLASRIERLP- 60

Query: 61  SSNFLLLWENSYYFIVFQLLEQSSNDYFTWAGENCNGNSQFEEVQAMLLAEFCLSVSFSD 120
                                                                  VSFSD
Sbjct: 61  -------------------------------------------------------VSFSD 120

Query: 121 SCIMSSCVKPSIQSEERPVVLLHGFDSSCLEWRYTYPLLEEAGLETWAVDILGWGFSDLE 180
           SCIMSSCVKPSIQS++ PVVLLHGFDSSCLEWRYTYPLLEEAGLETWAVD+LGWGFSDLE
Sbjct: 121 SCIMSSCVKPSIQSKKCPVVLLHGFDSSCLEWRYTYPLLEEAGLETWAVDVLGWGFSDLE 180

Query: 181 RLPPCDVTSKRVHLYQLWKSYIKRPMVLVGPSLGAAVAIDFAVNYPEAVDRLVLIDASVY 240
           RLPPCDVTSKRVHLYQLWKSYIKRPMV+VGPSLGAAVAIDFAVNYPEAVDRLVLIDASVY
Sbjct: 181 RLPPCDVTSKRVHLYQLWKSYIKRPMVIVGPSLGAAVAIDFAVNYPEAVDRLVLIDASVY 240

Query: 241 AEGTGNLATLPRLLAYAGVFLLKSIPLRLYVNVLTFTGISFSTGLDWANIGRLHCLLPWW 300
           AEGTGNLATLPRL+AYAGVFLLKSIPLR+YVN LTFT I FSTGLDWANIGRLHCLLPWW
Sbjct: 241 AEGTGNLATLPRLIAYAGVFLLKSIPLRVYVNFLTFTSIPFSTGLDWANIGRLHCLLPWW 300

Query: 301 ENATVSFMLSGGYNVSSQIKKVKQKTLIIWGEDDQIISNKLGVRLHCELPNAVIRQIPEC 360
           E+ATVSFMLSGGY VSSQI+KVKQKTLIIWGEDDQI+S KLGVRLHCELPNAV R I EC
Sbjct: 301 EDATVSFMLSGGYKVSSQIEKVKQKTLIIWGEDDQIVSYKLGVRLHCELPNAVFRPIAEC 331

Query: 361 GHLPHVEKPNPVAKLITQFVHEDSRKE 388
           GHLPHVEKPN VAKLITQF+HEDSRKE
Sbjct: 361 GHLPHVEKPNLVAKLITQFIHEDSRKE 331

BLAST of ClCG08G010370 vs. ExPASy TrEMBL
Match: A0A6J1HBV7 (uncharacterized protein LOC111462669 isoform X1 OS=Cucurbita moschata OX=3662 GN=LOC111462669 PE=4 SV=1)

HSP 1 Score: 612.8 bits (1579), Expect = 9.8e-172
Identity = 310/393 (78.88%), Postives = 319/393 (81.17%), Query Frame = 0

Query: 1   MLSLTMAASWGSAPLHIQWKKPKRLGVTVNEFPSFLPKEVHNIKDQFARNLATRIERLPV 60
           MLSL +AASW SAP  +QWKKPKRLGV +N+FPSFLPKEV NIKDQFAR LATRIERLP 
Sbjct: 1   MLSLAVAASWCSAPPLMQWKKPKRLGVILNKFPSFLPKEVDNIKDQFARELATRIERLP- 60

Query: 61  SSNFLLLWENSYYFIVFQLLEQSSNDYFTWAGENCNGNSQFEEVQAMLLAEFCLSVSFSD 120
                                                                  VSFSD
Sbjct: 61  -------------------------------------------------------VSFSD 120

Query: 121 SCIMSSCVKPSIQSEERPVVLLHGFDSSCLEWRYTYPLLEEAGLETWAVDILGWGFSDLE 180
           SC MSSCVKPSIQS+E PVVLLHGFDSSCLEWRYTYPLLEEAG ETWAVDILGWGFSDLE
Sbjct: 121 SCTMSSCVKPSIQSKECPVVLLHGFDSSCLEWRYTYPLLEEAGFETWAVDILGWGFSDLE 180

Query: 181 RLPPCDVTSKRVHLYQLWKSYIKRPMVLVGPSLGAAVAIDFAVNYPEAVDRLVLIDASVY 240
           RLPPCDVTSKRVHLYQLWKSYIKRPMVLVGPSLGAAVAIDFAVNYPEAVDRLVLIDASVY
Sbjct: 181 RLPPCDVTSKRVHLYQLWKSYIKRPMVLVGPSLGAAVAIDFAVNYPEAVDRLVLIDASVY 240

Query: 241 AEGTGNLATLPRLLAYAGVFLLKSIPLRLYVNVLTFTGISFSTGLDWANIGRLHCLLPWW 300
           AEGTGNLATLPRL+AYAGVFLLKSIPLRLYVNVL FTGIS ST LDW NIGRLHCLLPWW
Sbjct: 241 AEGTGNLATLPRLIAYAGVFLLKSIPLRLYVNVLAFTGISLSTSLDWVNIGRLHCLLPWW 300

Query: 301 ENATVSFMLSGGYNVSSQIKKVKQKTLIIWGEDDQIISNKLGVRLHCELPNAVIRQIPEC 360
           E+ATVSFMLSGGYNVSSQI+KVKQKTLIIWGEDDQIISNKL VRLHCELPNA IRQIPEC
Sbjct: 301 EDATVSFMLSGGYNVSSQIEKVKQKTLIIWGEDDQIISNKLAVRLHCELPNAFIRQIPEC 337

Query: 361 GHLPHVEKPNPVAKLITQFVHEDSRKEVQYVNS 394
           GHLPHVEKPN VAKLITQFVHEDSRKEV YVN+
Sbjct: 361 GHLPHVEKPNFVAKLITQFVHEDSRKEVPYVNN 337

BLAST of ClCG08G010370 vs. ExPASy TrEMBL
Match: A0A0A0KGX4 (AB hydrolase-1 domain-containing protein OS=Cucumis sativus OX=3659 GN=Csa_6G366450 PE=4 SV=1)

HSP 1 Score: 610.1 bits (1572), Expect = 6.3e-171
Identity = 306/393 (77.86%), Postives = 322/393 (81.93%), Query Frame = 0

Query: 1   MLSLTMAASWGSAPLHIQWKKPKRLGVTVNEFPSFLPKEVHNIKDQFARNLATRIERLPV 60
           MLS TMA++WGSAPLHIQWKKPKR GV +NE PSFLPKEV+NIKDQ ARNLA+RI+RLP 
Sbjct: 1   MLSPTMASTWGSAPLHIQWKKPKRPGVILNELPSFLPKEVYNIKDQSARNLASRIQRLP- 60

Query: 61  SSNFLLLWENSYYFIVFQLLEQSSNDYFTWAGENCNGNSQFEEVQAMLLAEFCLSVSFSD 120
                                                                  VSFSD
Sbjct: 61  -------------------------------------------------------VSFSD 120

Query: 121 SCIMSSCVKPSIQSEERPVVLLHGFDSSCLEWRYTYPLLEEAGLETWAVDILGWGFSDLE 180
            CIMSSCVKPSIQ+++ PVVLLHGFDSSCLEWRYTYPLLEEAGLETWAVDILGWGFSDLE
Sbjct: 121 FCIMSSCVKPSIQNKDCPVVLLHGFDSSCLEWRYTYPLLEEAGLETWAVDILGWGFSDLE 180

Query: 181 RLPPCDVTSKRVHLYQLWKSYIKRPMVLVGPSLGAAVAIDFAVNYPEAVDRLVLIDASVY 240
           RLPPCDVTSKRVHLYQLWKSYIK+PMV+VGPSLGAAVAIDFAVNYPEAVDRLVLIDASVY
Sbjct: 181 RLPPCDVTSKRVHLYQLWKSYIKKPMVIVGPSLGAAVAIDFAVNYPEAVDRLVLIDASVY 240

Query: 241 AEGTGNLATLPRLLAYAGVFLLKSIPLRLYVNVLTFTGISFSTGLDWANIGRLHCLLPWW 300
           AEGTGNLATLPR +AYAGVFLLKSIPLR+YVNVLTFTGI FST LDWANIGRLHCLLPWW
Sbjct: 241 AEGTGNLATLPRSIAYAGVFLLKSIPLRVYVNVLTFTGIPFSTSLDWANIGRLHCLLPWW 300

Query: 301 ENATVSFMLSGGYNVSSQIKKVKQKTLIIWGEDDQIISNKLGVRLHCELPNAVIRQIPEC 360
           E+ATVSFMLSGGY VSSQI+KVKQKTLIIWGEDDQIIS KLGVRLHCELPNAVIR I EC
Sbjct: 301 EDATVSFMLSGGYKVSSQIEKVKQKTLIIWGEDDQIISYKLGVRLHCELPNAVIRPIAEC 337

Query: 361 GHLPHVEKPNPVAKLITQFVHEDSRKEVQYVNS 394
           GHLPHVEKPN VAKLITQFVHEDSRKEVQ+VNS
Sbjct: 361 GHLPHVEKPNLVAKLITQFVHEDSRKEVQFVNS 337

BLAST of ClCG08G010370 vs. ExPASy TrEMBL
Match: A0A6J1KAS9 (uncharacterized protein LOC111492171 isoform X2 OS=Cucurbita maxima OX=3661 GN=LOC111492171 PE=4 SV=1)

HSP 1 Score: 608.2 bits (1567), Expect = 2.4e-170
Identity = 307/388 (79.12%), Postives = 316/388 (81.44%), Query Frame = 0

Query: 1   MLSLTMAASWGSAPLHIQWKKPKRLGVTVNEFPSFLPKEVHNIKDQFARNLATRIERLPV 60
           MLSL +AASW SAP  +QWKKPKRLGV +N+FPSFLPKEV NIKDQFAR LATRIERLP 
Sbjct: 1   MLSLAVAASWCSAPPLMQWKKPKRLGVIINKFPSFLPKEVDNIKDQFARELATRIERLP- 60

Query: 61  SSNFLLLWENSYYFIVFQLLEQSSNDYFTWAGENCNGNSQFEEVQAMLLAEFCLSVSFSD 120
                                                                  VSFSD
Sbjct: 61  -------------------------------------------------------VSFSD 120

Query: 121 SCIMSSCVKPSIQSEERPVVLLHGFDSSCLEWRYTYPLLEEAGLETWAVDILGWGFSDLE 180
           SCIMSSCVKPSIQS+E PVVLLHGFDSSCLEWRYTYPLLEEAG ETWAVDILGWGFSDLE
Sbjct: 121 SCIMSSCVKPSIQSKECPVVLLHGFDSSCLEWRYTYPLLEEAGFETWAVDILGWGFSDLE 180

Query: 181 RLPPCDVTSKRVHLYQLWKSYIKRPMVLVGPSLGAAVAIDFAVNYPEAVDRLVLIDASVY 240
           RLPPCDVTSKRVHLYQLWKSYIKRPMV+VGPSLGAAVAIDFAVNYPEAVDRLVLIDASVY
Sbjct: 181 RLPPCDVTSKRVHLYQLWKSYIKRPMVMVGPSLGAAVAIDFAVNYPEAVDRLVLIDASVY 240

Query: 241 AEGTGNLATLPRLLAYAGVFLLKSIPLRLYVNVLTFTGISFSTGLDWANIGRLHCLLPWW 300
           AEGTGNLATLPRL+AYAGVFLLKSIPLRLYVNVL FTGIS ST LDW NIGRLHCLLPWW
Sbjct: 241 AEGTGNLATLPRLIAYAGVFLLKSIPLRLYVNVLAFTGISLSTSLDWVNIGRLHCLLPWW 300

Query: 301 ENATVSFMLSGGYNVSSQIKKVKQKTLIIWGEDDQIISNKLGVRLHCELPNAVIRQIPEC 360
           E+ATVSFMLSGGYNVSSQI+KVKQKTLIIWGEDDQIISNKL VRLHCELPNA IRQIPEC
Sbjct: 301 EDATVSFMLSGGYNVSSQIEKVKQKTLIIWGEDDQIISNKLAVRLHCELPNAFIRQIPEC 332

Query: 361 GHLPHVEKPNPVAKLITQFVHEDSRKEV 389
           GHLPHVEKPN VAKLITQFVHEDSRKEV
Sbjct: 361 GHLPHVEKPNFVAKLITQFVHEDSRKEV 332

BLAST of ClCG08G010370 vs. TAIR 10
Match: AT1G13820.1 (alpha/beta-Hydrolases superfamily protein )

HSP 1 Score: 460.3 bits (1183), Expect = 1.6e-129
Identity = 237/393 (60.31%), Postives = 268/393 (68.19%), Query Frame = 0

Query: 1   MLSLTMAASWGSAPLHIQWKKPKRLGVTVNEFPSFLPKEVHNIKDQFARNLATRIERLPV 60
           MLS   A +     L ++    +   VT  +FP+FLP +V  IKD FA  LA RIERLPV
Sbjct: 1   MLSTVTATAVSRPILKVECTIKRTFKVTAEKFPTFLPTDVEKIKDTFALKLAARIERLPV 60

Query: 61  SSNFLLLWENSYYFIVFQLLEQSSNDYFTWAGENCNGNSQFEEVQAMLLAEFCLSVSFSD 120
                                                                 SVSF +
Sbjct: 61  ------------------------------------------------------SVSFRE 120

Query: 121 SCIMSSCVKPSIQSEERPVVLLHGFDSSCLEWRYTYPLLEEAGLETWAVDILGWGFSDLE 180
             IMSSCV P +++E  PVVLLHGFDSSCLEWRYTYPLLEEAGLETWA DILGWGFSDL+
Sbjct: 121 DSIMSSCVTPLMRNETTPVVLLHGFDSSCLEWRYTYPLLEEAGLETWAFDILGWGFSDLD 180

Query: 181 RLPPCDVTSKRVHLYQLWKSYIKRPMVLVGPSLGAAVAIDFAVNYPEAVDRLVLIDASVY 240
           +LPPCDV SKR H Y+ WKS+IKRP+VLVGPSLGAAVAID AVN+PEAV+ LVL+DASVY
Sbjct: 181 KLPPCDVASKREHFYKFWKSHIKRPVVLVGPSLGAAVAIDIAVNHPEAVESLVLMDASVY 240

Query: 241 AEGTGNLATLPRLLAYAGVFLLKSIPLRLYVNVLTFTGISFSTGLDWANIGRLHCLLPWW 300
           AEGTGNLATLP+  AYAGV+LLKSIPLRLYVN + F GIS  T  DW  IGRLHCL PWW
Sbjct: 241 AEGTGNLATLPKAAAYAGVYLLKSIPLRLYVNFICFNGISLETSWDWTKIGRLHCLYPWW 300

Query: 301 ENATVSFMLSGGYNVSSQIKKVKQKTLIIWGEDDQIISNKLGVRLHCELPNAVIRQIPEC 360
           E+ATVSFM SGGYNV+S IKKV QKTLI+WGEDDQIISNKL  RLH EL NA ++QI  C
Sbjct: 301 EDATVSFMTSGGYNVTSLIKKVSQKTLILWGEDDQIISNKLAWRLHGELSNARVKQISNC 339

Query: 361 GHLPHVEKPNPVAKLITQFVHEDSR-KEVQYVN 393
           GHLPHVEKP  V KLI +FV E  R KEV+ ++
Sbjct: 361 GHLPHVEKPAAVTKLIAEFVRETCRCKEVESIS 339

BLAST of ClCG08G010370 vs. TAIR 10
Match: AT5G39220.1 (alpha/beta-Hydrolases superfamily protein )

HSP 1 Score: 396.4 bits (1017), Expect = 2.8e-110
Identity = 193/358 (53.91%), Postives = 238/358 (66.48%), Query Frame = 0

Query: 23  KRLGVTVNEFPSFLPKEVHNIKDQFARNLATRIERLPVSSNFLLLWENSYYFIVFQLLEQ 82
           +R+    + FP+FLPKE+ NIKD FAR LA RI R+PV                      
Sbjct: 19  QRVTANGDGFPTFLPKEIQNIKDPFARALAQRIVRIPVP--------------------- 78

Query: 83  SSNDYFTWAGENCNGNSQFEEVQAMLLAEFCLSVSFSDSCIMSSCVKPSIQSEER-PVVL 142
                                          L +      +MSSC+KP +Q  ++ PVVL
Sbjct: 79  -------------------------------LQMGNFRGSVMSSCIKPLVQLHDKSPVVL 138

Query: 143 LHGFDSSCLEWRYTYPLLEEAGLETWAVDILGWGFSDLERLPPCDVTSKRVHLYQLWKSY 202
           LH FDSSCLEWR TYPLLE+A LETWA+D+LGWGFSDLE+LPPCD  SKR HL++LWK+Y
Sbjct: 139 LHCFDSSCLEWRRTYPLLEQACLETWAIDVLGWGFSDLEKLPPCDAASKRHHLFELWKTY 198

Query: 203 IKRPMVLVGPSLGAAVAIDFAVNYPEAVDRLVLIDASVYAEGTGNLATLPRLLAYAGVFL 262
           IKRPM+LVGPSLGA VA+DF   YPEAVD+LVLI+A+ Y+EGTG L  LP+ +AYAGV L
Sbjct: 199 IKRPMILVGPSLGATVAVDFTATYPEAVDKLVLINANAYSEGTGRLKELPKSIAYAGVKL 258

Query: 263 LKSIPLRLYVNVLTFTGISFSTGLDWANIGRLHCLLPWWENATVSFMLSGGYNVSSQIKK 322
           LKS PLRL  NVL F     S  +DW NIGRLHC +PWWE+A V FM+SGGYNV+S IK 
Sbjct: 259 LKSFPLRLLANVLAFCSSPLSENIDWTNIGRLHCQMPWWEDAMVDFMISGGYNVASHIKH 318

Query: 323 VKQKTLIIWGEDDQIISNKLGVRLHCELPNAVIRQIPECGHLPHVEKPNPVAKLITQF 380
           +  KTL++  E+DQI+SN+L V+L CEL NAV+R++P+ GHLPHVE P  + KLI+ F
Sbjct: 319 IDHKTLVVCSENDQIVSNQLSVKLLCELANAVLREVPDSGHLPHVENPKHIVKLISDF 324

BLAST of ClCG08G010370 vs. TAIR 10
Match: AT5G09430.1 (alpha/beta-Hydrolases superfamily protein )

HSP 1 Score: 76.3 bits (186), Expect = 6.3e-14
Identity = 73/264 (27.65%), Postives = 120/264 (45.45%), Query Frame = 0

Query: 127 CVKPSIQSEERP-VVLLHGFDSSCLEWRYTYPLLEEAG-LETWAVDILGWGFSDLERLPP 186
           C  P   +  +P ++LLHGF ++ + W+Y   L    G    +  D+L +G S       
Sbjct: 50  CWIPKSPNRSKPNLLLLHGFGANAM-WQYGEHLRAFTGRFNVYVPDLLFFGLSSTSEPNR 109

Query: 187 CDVTSKRVHLYQLWKSYIKRPMVLVGPSLGAAVAIDFAVNYPEAVDRLVLIDASVYAEG- 246
            +    R  L +L +++  + M +VG S G  V    A  +PE V++LVL  A V  E  
Sbjct: 110 TESFQARC-LMRLMEAHGVQRMNIVGISYGGFVGYSLAAQFPENVEKLVLCCAGVCLEEK 169

Query: 247 --TGNLATLPRLLAYAGVFLLKSIPLRLYVNVLTFTGISFSTGLD----WANIGRLHCLL 306
                L  +P L    G+ L+   P +L   ++ F+ +    G+     W  I  +    
Sbjct: 170 DMEDGLFKVPNLEEATGI-LIPQTPEKL-KELIRFSFVKPIKGVPSFFLWDFIDVMCTEF 229

Query: 307 PWWENATVSFMLSGGYNVSSQIKKVKQKTLIIWGEDDQIISNKLGVRLHCEL-PNAVIRQ 366
              +   +  +L       S + ++KQK+LIIWGE+DQI   +LG RL   +  +A I  
Sbjct: 230 VEEKRDLIKSILKD--RRLSDLPRIKQKSLIIWGEEDQIFPLELGYRLKRHIGESAEIVV 289

Query: 367 IPECGHLPHVEKPNPVAKLITQFV 381
           I + GH  ++EK     K +  F+
Sbjct: 290 IKKAGHAVNLEKSKEFVKHLKSFL 307

BLAST of ClCG08G010370 vs. TAIR 10
Match: AT4G36530.1 (alpha/beta-Hydrolases superfamily protein )

HSP 1 Score: 74.7 bits (182), Expect = 1.8e-13
Identity = 39/107 (36.45%), Postives = 60/107 (56.07%), Query Frame = 0

Query: 132 IQSEERPVVLLHGFDSSCLEWRYTYPLLEEAGLETWAVDILGWGFSDLERLPPCDVTSKR 191
           +Q E  P+VL+HGF +S   WRY  P L +   + +A+D+LG+G+SD + L   D     
Sbjct: 38  VQGEGSPLVLIHGFGASVFHWRYNIPELAKK-YKVYALDLLGFGWSD-KALIEYDAMVWT 97

Query: 192 VHLYQLWKSYIKRPMVLVGPSLGAAVAIDFAVNYPEAVDRLVLIDAS 239
             +    K  +K P V+VG SLG   A+  AV  PE V  + L++++
Sbjct: 98  DQVIDFMKEVVKEPAVVVGNSLGGFTALSVAVGLPEQVTGVALLNSA 142

BLAST of ClCG08G010370 vs. TAIR 10
Match: AT4G36530.2 (alpha/beta-Hydrolases superfamily protein )

HSP 1 Score: 74.7 bits (182), Expect = 1.8e-13
Identity = 39/107 (36.45%), Postives = 60/107 (56.07%), Query Frame = 0

Query: 132 IQSEERPVVLLHGFDSSCLEWRYTYPLLEEAGLETWAVDILGWGFSDLERLPPCDVTSKR 191
           +Q E  P+VL+HGF +S   WRY  P L +   + +A+D+LG+G+SD + L   D     
Sbjct: 95  VQGEGSPLVLIHGFGASVFHWRYNIPELAKK-YKVYALDLLGFGWSD-KALIEYDAMVWT 154

Query: 192 VHLYQLWKSYIKRPMVLVGPSLGAAVAIDFAVNYPEAVDRLVLIDAS 239
             +    K  +K P V+VG SLG   A+  AV  PE V  + L++++
Sbjct: 155 DQVIDFMKEVVKEPAVVVGNSLGGFTALSVAVGLPEQVTGVALLNSA 199

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
XP_038884275.11.2e-17981.68uncharacterized hydrolase YugF isoform X2 [Benincasa hispida][more]
XP_038884274.12.1e-17678.29uncharacterized hydrolase YugF isoform X1 [Benincasa hispida][more]
KAA0064761.16.3e-17378.37putative hydrolase YugF [Cucumis melo var. makuwa][more]
XP_008445456.11.5e-17178.81PREDICTED: uncharacterized hydrolase YugF [Cucumis melo][more]
KAG7029571.12.0e-17178.88yugF, partial [Cucurbita argyrosperma subsp. argyrosperma][more]
Match NameE-valueIdentityDescription
O052356.6e-1626.02Uncharacterized hydrolase YugF OS=Bacillus subtilis (strain 168) OX=224308 GN=yu... [more]
Q476M76.8e-1327.692-hydroxy-6-oxononadienedioate/2-hydroxy-6-oxononatrienedioate hydrolase OS=Cupr... [more]
Q8KZP54.4e-1231.492-hydroxy-6-oxononadienedioate/2-hydroxy-6-oxononatrienedioate hydrolase OS=Coma... [more]
Q2VLB94.9e-1129.892-hydroxy-6-oxo-6-phenylhexa-2,4-dienoate hydrolase OS=Burkholderia cepacia OX=2... [more]
P175485.4e-1029.832-hydroxy-6-oxo-6-phenylhexa-2,4-dienoate hydrolase OS=Pseudomonas sp. (strain K... [more]
Match NameE-valueIdentityDescription
A0A5A7VGC53.0e-17378.37Putative hydrolase YugF OS=Cucumis melo var. makuwa OX=1194695 GN=E6C27_scaffold... [more]
A0A1S3BDL97.5e-17278.81uncharacterized hydrolase YugF OS=Cucumis melo OX=3656 GN=LOC103488473 PE=4 SV=1[more]
A0A6J1HBV79.8e-17278.88uncharacterized protein LOC111462669 isoform X1 OS=Cucurbita moschata OX=3662 GN... [more]
A0A0A0KGX46.3e-17177.86AB hydrolase-1 domain-containing protein OS=Cucumis sativus OX=3659 GN=Csa_6G366... [more]
A0A6J1KAS92.4e-17079.12uncharacterized protein LOC111492171 isoform X2 OS=Cucurbita maxima OX=3661 GN=L... [more]
Match NameE-valueIdentityDescription
AT1G13820.11.6e-12960.31alpha/beta-Hydrolases superfamily protein [more]
AT5G39220.12.8e-11053.91alpha/beta-Hydrolases superfamily protein [more]
AT5G09430.16.3e-1427.65alpha/beta-Hydrolases superfamily protein [more]
AT4G36530.11.8e-1336.45alpha/beta-Hydrolases superfamily protein [more]
AT4G36530.21.8e-1336.45alpha/beta-Hydrolases superfamily protein [more]
InterPro
Analysis Name: InterPro Annotations of Watermelon (Charleston Gray) v2.5
Date Performed: 2022-01-31
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR000073Alpha/beta hydrolase fold-1PRINTSPR00111ABHYDROLASEcoord: 326..340
score: 41.84
coord: 222..235
score: 43.35
coord: 208..221
score: 42.86
coord: 163..178
score: 33.84
IPR000073Alpha/beta hydrolase fold-1PFAMPF12697Abhydrolase_6coord: 139..374
e-value: 3.5E-15
score: 57.2
IPR000639Epoxide hydrolase-likePRINTSPR00412EPOXHYDRLASEcoord: 143..161
score: 36.84
coord: 163..178
score: 37.22
coord: 357..379
score: 27.67
coord: 222..235
score: 29.22
IPR029058Alpha/Beta hydrolase foldGENE3D3.40.50.1820alpha/beta hydrolasecoord: 129..389
e-value: 6.5E-42
score: 146.3
IPR029058Alpha/Beta hydrolase foldSUPERFAMILY53474alpha/beta-Hydrolasescoord: 137..380
NoneNo IPR availablePANTHERPTHR43689HYDROLASEcoord: 112..382
NoneNo IPR availablePANTHERPTHR43689HYDROLASEcoord: 26..61
NoneNo IPR availablePANTHERPTHR43689:SF37ALPHA/BETA-HYDROLASES SUPERFAMILY PROTEINcoord: 26..61
NoneNo IPR availablePANTHERPTHR43689:SF37ALPHA/BETA-HYDROLASES SUPERFAMILY PROTEINcoord: 112..382

Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
ClCG08G010370.2ClCG08G010370.2mRNA


GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
molecular_function GO:0016787 hydrolase activity
molecular_function GO:0003824 catalytic activity