ClCG08G009620 (gene) Watermelon (Charleston Gray) v2.5

BLAST of ClCG08G009620 vs. NCBI nr
Match: XP_038885883.1 (neutral ceramidase 2-like [Benincasa hispida])

HSP 1 Score: 1367.4 bits (3538), Expect = 0.0e+00
Identity = 676/738 (91.60%), Postives = 702/738 (95.12%), Query Frame = 0

Query: 12  MATVIVA----VNGDWLVGVGSFDMTGPAAGVNMMGYANMDQNTAGIHFRLRARSFVVAK 71
           MATVI A    VNGDWLVGVGSFDMTGPAAGVNM+GYANMDQNTAGIHFRLRAR+FVVAK
Sbjct: 1   MATVIAAVSWCVNGDWLVGVGSFDMTGPAAGVNMIGYANMDQNTAGIHFRLRARTFVVAK 60

Query: 72  SVDGPRIAFVNLDAGMASQLVTIKVLERLKSRFGNLYTEENVAISGIHTHAGPGGYLQYL 131
           +VDGPRIAF+NLDAGMASQLVTIKVLERLKSRFG+LYTEENVAISGIHTHAGPGGYLQYL
Sbjct: 61  TVDGPRIAFINLDAGMASQLVTIKVLERLKSRFGDLYTEENVAISGIHTHAGPGGYLQYL 120

Query: 132 VYSITSLGFVQQSFDAIVDAIEQSIVQAHESLKPGFILFNKGDVANAGINRSPSAYLMNP 191
           VYSITSLGFVQQSFDAIV+AIEQSI+QAHESLKPGFILFNKGDV N GINRSPSAYLMNP
Sbjct: 121 VYSITSLGFVQQSFDAIVNAIEQSIIQAHESLKPGFILFNKGDVENGGINRSPSAYLMNP 180

Query: 192 QQERDRYATNVDKEMTVLKFVDGGSGKNIGAFSWFPTHGTSMSRDNKLISGDNKGAAARF 251
           ++ER RYA NVDKEM+VLKFVDG SG +IGAFSWFPTHGTSMSRDNKLISGDNKGAAARF
Sbjct: 181 EEERARYANNVDKEMSVLKFVDGESGNSIGAFSWFPTHGTSMSRDNKLISGDNKGAAARF 240

Query: 252 FEDWVASNHTTTTFSNHTSDIVELMKKAETIKATGGKRCSKTSSQASKVRKNDGSLFVAA 311
           FEDWVAS  T  T SN+TS IVELM+KAETIKATGGKRCSKTSSQASKVRKNDGSLFVAA
Sbjct: 241 FEDWVASKRTKITSSNNTSGIVELMRKAETIKATGGKRCSKTSSQASKVRKNDGSLFVAA 300

Query: 312 FCQSNVGDVTPNVLGAFCTDSGKPCDFNRSSCHGSDLLCVGRGPGFPDEIRSTKIIGERQ 371
           FCQSNVGDVTPNVLGAFC DSGKPCDFNRSSCHGSDLLCVGRGPGFPDEI STKIIGERQ
Sbjct: 301 FCQSNVGDVTPNVLGAFCIDSGKPCDFNRSSCHGSDLLCVGRGPGFPDEILSTKIIGERQ 360

Query: 372 FQKAAELFTTATEKLTGEIDFRHVYLNFTDIQVEIDGNNVVKTCPAALGPGFAAGTTDGP 431
           FQKAA+LFTTATEKLTGEIDFRHVYLNFTDI+VE+DGNNVVKTCPAALGPGFAAGTTDGP
Sbjct: 361 FQKAADLFTTATEKLTGEIDFRHVYLNFTDIEVELDGNNVVKTCPAALGPGFAAGTTDGP 420

Query: 432 GVFGFQQGDTEINKLWKKLRDSLRKPSEFQVDCQKPKPVLLDTGEMFKPYAWAPAILPIQ 491
           GVFGFQQGDTEINKLWKKLRDSL+KPSEFQV CQKPK VLLDTGEMF+PYAWAPAILPIQ
Sbjct: 421 GVFGFQQGDTEINKLWKKLRDSLKKPSEFQVGCQKPKTVLLDTGEMFEPYAWAPAILPIQ 480

Query: 492 ILRLGKLIILSVPGEFTTMAGRRLREAVKETLISNGNGEFDDDTHVVIAGLTNAYSQYVA 551
           ILRLGKLIILSVPGEFTTMAGR+LREAVKETLI NGNGEFDDDTHVVIAGLTN YSQYVA
Sbjct: 481 ILRLGKLIILSVPGEFTTMAGRQLREAVKETLIRNGNGEFDDDTHVVIAGLTNTYSQYVA 540

Query: 552 TFEEYEQQRYEAASTLYGPHTLSAYIQEFKKLATAIAKGEKIVKPIASPPDLSSVQLRFV 611
           TFEEYEQQRYEAASTLYGPHTLSAYIQEFKKLA AIAK E I KPIASPPDLSSVQLRFV
Sbjct: 541 TFEEYEQQRYEAASTLYGPHTLSAYIQEFKKLAIAIAKHENIAKPIASPPDLSSVQLRFV 600

Query: 612 LDPFGESPPNGTNFGDIKQDVNLPENGWFKRGGIQKPTATFWSANPRFDLLTEGTYAVVE 671
           +DPFGESPPNG NFGDI+QD+ LP NGWFKRGG QKP ATFWSANPRFDLLTEGTYAVVE
Sbjct: 601 IDPFGESPPNGINFGDIQQDIKLPTNGWFKRGGKQKPAATFWSANPRFDLLTEGTYAVVE 660

Query: 672 KLEKQRWTPVYDDDDISLFFKWTLD-NTFMNSLVTIEWDIPINADPGVYRLRHFGSSKST 731
           +LEKQRWTPVYDDDD SLFFKWTLD NTF+NS+ TIEWDIPI+ADPGVYRLRHFGSSKST
Sbjct: 661 RLEKQRWTPVYDDDDFSLFFKWTLDNNTFINSIATIEWDIPIDADPGVYRLRHFGSSKST 720

Query: 732 INSTNTYFTGASQAFAVF 745
           INSTN YFTGASQAFAVF
Sbjct: 721 INSTNIYFTGASQAFAVF 738

BLAST of ClCG08G009620 vs. NCBI nr
Match: XP_031742885.1 (neutral ceramidase 2 [Cucumis sativus])

HSP 1 Score: 1332.0 bits (3446), Expect = 0.0e+00
Identity = 660/743 (88.83%), Postives = 695/743 (93.54%), Query Frame = 0

Query: 12  MATVIVA----VNGDWLVGVGSFDMTGPAAGVNMMGYANMDQNTAGIHFRLRARSFVVAK 71
           MATV+VA    VNGDWLVGVGSFDMTGPAA VNMMGYANMDQNTAGIHFRLRAR+FVVA 
Sbjct: 1   MATVVVAVSWCVNGDWLVGVGSFDMTGPAAEVNMMGYANMDQNTAGIHFRLRARTFVVAS 60

Query: 72  SVDGPRIAFVNLDAGMASQLVTIKVLERLKS-----RFGNLYTEENVAISGIHTHAGPGG 131
           SVDGPRIAFVNLDAGMASQLVTIKVLERLKS     RFG++YTEENVAISGIHTHAGPGG
Sbjct: 61  SVDGPRIAFVNLDAGMASQLVTIKVLERLKSSILVQRFGDVYTEENVAISGIHTHAGPGG 120

Query: 132 YLQYLVYSITSLGFVQQSFDAIVDAIEQSIVQAHESLKPGFILFNKGDVANAGINRSPSA 191
           YLQYLVYSITSLGFVQQSFDAIV+AIEQSI+QAHESLKPGFILFNKG+V NAGINRSPSA
Sbjct: 121 YLQYLVYSITSLGFVQQSFDAIVNAIEQSIIQAHESLKPGFILFNKGEVENAGINRSPSA 180

Query: 192 YLMNPQQERDRYATNVDKEMTVLKFVDGGSGKNIGAFSWFPTHGTSMSRDNKLISGDNKG 251
           YLMNP++ER +YA NVDKEM++LKFVDG +G +IGAFSWFPTHGTSMSRDNKLISGDNKG
Sbjct: 181 YLMNPEEERAKYANNVDKEMSILKFVDGENGNSIGAFSWFPTHGTSMSRDNKLISGDNKG 240

Query: 252 AAARFFEDWVASN-HTTTTFSNHTSDIVELMKKAETIKATGGKRCSKTSSQASKVRKNDG 311
           AAARFFEDW ASN  TTTT SN+TS I +LMKKA  IKATGGKRCSKTSS++SKVRKNDG
Sbjct: 241 AAARFFEDWAASNRRTTTTPSNNTSGIEDLMKKAGKIKATGGKRCSKTSSKSSKVRKNDG 300

Query: 312 SLFVAAFCQSNVGDVTPNVLGAFCTDSGKPCDFNRSSCHGSDLLCVGRGPGFPDEIRSTK 371
           SLFV AFCQSNVGDVTPNVLGAFCTDSG PCDFN SSCHGSDLLCVGRGPGFPDEI STK
Sbjct: 301 SLFVGAFCQSNVGDVTPNVLGAFCTDSGNPCDFNHSSCHGSDLLCVGRGPGFPDEILSTK 360

Query: 372 IIGERQFQKAAELFTTATEKLTGEIDFRHVYLNFTDIQVEIDGNNVVKTCPAALGPGFAA 431
           IIGERQF KAA+LFTTATEKLTGEIDFRHVYLNFTDI+V IDGNNVV+TCPAALGPGFAA
Sbjct: 361 IIGERQFLKAADLFTTATEKLTGEIDFRHVYLNFTDIEVAIDGNNVVRTCPAALGPGFAA 420

Query: 432 GTTDGPGVFGFQQGDTEINKLWKKLRDSLRKPSEFQVDCQKPKPVLLDTGEMFKPYAWAP 491
           GTTDGPG FGFQQGDTEINKLWK+LRDSL+KPSEFQV CQKPK VLLDTGEMF+PYAWAP
Sbjct: 421 GTTDGPGAFGFQQGDTEINKLWKQLRDSLKKPSEFQVGCQKPKTVLLDTGEMFEPYAWAP 480

Query: 492 AILPIQILRLGKLIILSVPGEFTTMAGRRLREAVKETLISNGNGEFDDDTHVVIAGLTNA 551
           AILPIQILRLG LIILSVPGEFTTMAGRRLREAVKETLISNGNG FDDDTHVVIAGLTN 
Sbjct: 481 AILPIQILRLGNLIILSVPGEFTTMAGRRLREAVKETLISNGNGVFDDDTHVVIAGLTNT 540

Query: 552 YSQYVATFEEYEQQRYEAASTLYGPHTLSAYIQEFKKLATAIAKGEKIVKPIASPPDLSS 611
           YSQYVATFEEYEQQRYEAASTLYGPHTLSAYIQEFKKLA AIAKGEKI KPIASPPDLSS
Sbjct: 541 YSQYVATFEEYEQQRYEAASTLYGPHTLSAYIQEFKKLAIAIAKGEKIAKPIASPPDLSS 600

Query: 612 VQLRFVLDPFGESPPNGTNFGDIKQDVNLPENGWFKRGGIQKPTATFWSANPRFDLLTEG 671
           VQLR V+DPFGESPPNG NFGDI+QDV LP++GWFK G  QKPTATFWSANPRFDLLTEG
Sbjct: 601 VQLRLVMDPFGESPPNGVNFGDIQQDVELPKDGWFKTGSKQKPTATFWSANPRFDLLTEG 660

Query: 672 TYAVVEKLEKQRWTPVYDDDDISLFFKWTLDNTFMNSLVTIEWDIPINADPGVYRLRHFG 731
           TYA+VE+LEKQRWTP YDDDD SLFFKW LDNTF+N+LVTIEWDIPI+ADPGVYRLRHFG
Sbjct: 661 TYALVERLEKQRWTPAYDDDDFSLFFKWKLDNTFINNLVTIEWDIPIDADPGVYRLRHFG 720

Query: 732 SSKSTINSTNTYFTGASQAFAVF 745
           SS+STINSTN YFTGAS+AFAVF
Sbjct: 721 SSRSTINSTNVYFTGASRAFAVF 743

BLAST of ClCG08G009620 vs. NCBI nr
Match: XP_008464893.1 (PREDICTED: neutral ceramidase-like [Cucumis melo])

HSP 1 Score: 1320.4 bits (3416), Expect = 0.0e+00
Identity = 657/748 (87.83%), Postives = 697/748 (93.18%), Query Frame = 0

Query: 12  MATVIV----AVNGDWLVGVGSFDMTGPAAGVNMMGYANMDQNTAGIHFRLRARSFVVAK 71
           MA V++     VNGDWLVGVGSFDMTGPAA VNMMGYANMDQ TAGIHFRLRAR+FVVA+
Sbjct: 1   MAIVVLDVSWCVNGDWLVGVGSFDMTGPAAEVNMMGYANMDQVTAGIHFRLRARTFVVAR 60

Query: 72  SVDGPRIAFVNLDAGMASQLVTIKVLERLKS---------RFGNLYTEENVAISGIHTHA 131
           S+DGPRIAFVNLDAGMASQLVTIKVLERL+S         RFG+LYTEENVAISGIHTHA
Sbjct: 61  SIDGPRIAFVNLDAGMASQLVTIKVLERLQSRRNINISVQRFGDLYTEENVAISGIHTHA 120

Query: 132 GPGGYLQYLVYSITSLGFVQQSFDAIVDAIEQSIVQAHESLKPGFILFNKGDVANAGINR 191
           GPGGYLQYLVYSITSLGFVQQSFDAIV+AIEQSI+QAHESLKPGFILFNKG+V NAGINR
Sbjct: 121 GPGGYLQYLVYSITSLGFVQQSFDAIVNAIEQSIIQAHESLKPGFILFNKGEVDNAGINR 180

Query: 192 SPSAYLMNPQQERDRYATNVDKEMTVLKFVDGGSGKNIGAFSWFPTHGTSMSRDNKLISG 251
           SPSAYLMNP++ER +YA NVDKEM++LKFVDG SG +IGAFSWFPTHGTSMSRDNKLISG
Sbjct: 181 SPSAYLMNPEEERAKYANNVDKEMSILKFVDGESGNSIGAFSWFPTHGTSMSRDNKLISG 240

Query: 252 DNKGAAARFFEDWVASN-HTTTTFSNHTSDIVELMKKAETIKATGGKRCSKTSSQASKVR 311
           DNKGAAARFFEDW ASN  TTTT SN+ S I ELMKKAE IKATGGK+CSKTSS++SKVR
Sbjct: 241 DNKGAAARFFEDWAASNGRTTTTPSNNASVIEELMKKAEKIKATGGKQCSKTSSKSSKVR 300

Query: 312 KNDGSLFVAAFCQSNVGDVTPNVLGAFCTDSGKPCDFNRSSCHGSDLLCVGRGPGFPDEI 371
           KNDGSLFVAAFCQSNVGDVTPNVLGAFC DSGKPCDFNRSSCHGSDLLCVGRGPGFPDEI
Sbjct: 301 KNDGSLFVAAFCQSNVGDVTPNVLGAFCIDSGKPCDFNRSSCHGSDLLCVGRGPGFPDEI 360

Query: 372 RSTKIIGERQFQKAAELFTTATEKLTGEIDFRHVYLNFTDIQVEIDGNNVVKTCPAALGP 431
            STKIIGERQF KAA+LFTTATEKLTGEIDFRHVYLNFTDI+VEIDGNNVV+TCPAALGP
Sbjct: 361 LSTKIIGERQFLKAADLFTTATEKLTGEIDFRHVYLNFTDIEVEIDGNNVVRTCPAALGP 420

Query: 432 GFAAGTTDGPGVFGFQQGDTEINKLWKKLRDSLRKPSEFQVDCQKPKPVLLDTGEMFKPY 491
           GFAAGTTDGPGVFGFQQGDTEINKLWK+LRDSL+KPSEFQV CQKPK VLLDTGEMF+PY
Sbjct: 421 GFAAGTTDGPGVFGFQQGDTEINKLWKQLRDSLKKPSEFQVGCQKPKTVLLDTGEMFEPY 480

Query: 492 AWAPAILPIQILRLGKLIILSVPGEFTTMAGRRLREAVKETLISNGNGEFDDDTHVVIAG 551
           AWAPAILPIQILRLGKLIILSVPGEFTTMAGRRLREAVKETLISNGNGEFDDDTHVVIAG
Sbjct: 481 AWAPAILPIQILRLGKLIILSVPGEFTTMAGRRLREAVKETLISNGNGEFDDDTHVVIAG 540

Query: 552 LTNAYSQYVATFEEYEQQRYEAASTLYGPHTLSAYIQEFKKLATAIAKGEKIVKPIASPP 611
           LTN YSQYVATFEEYEQQRYEAASTLYGPHTLSAYIQEFKKLA+AIAKGEK+ KPIASPP
Sbjct: 541 LTNTYSQYVATFEEYEQQRYEAASTLYGPHTLSAYIQEFKKLASAIAKGEKVAKPIASPP 600

Query: 612 DLSSVQLRFVLDPFGESPPNGTNFGDIKQDVNLPENGWFKRGGIQKPTATFWSANPRFDL 671
           DLSSVQLR V+DPFGESPP+G NFGDI+QDV LP++GWFK G  QKPTATFWSANPRFDL
Sbjct: 601 DLSSVQLRLVMDPFGESPPDGVNFGDIQQDVELPKDGWFKTGSKQKPTATFWSANPRFDL 660

Query: 672 LTEGTYAVVEKLEKQRWTPVYDDDDISLFFKWTLDN-TFMNSLVTIEWDIPINADPGVYR 731
           LTEGTYA+VE+LEKQRWTP YDDDD S+ FKW LDN T +NSL TIEWDIPI+ADPGVYR
Sbjct: 661 LTEGTYALVERLEKQRWTPAYDDDDFSVSFKWKLDNTTLINSLATIEWDIPIDADPGVYR 720

Query: 732 LRHFGSSKSTINSTNTYFTGASQAFAVF 745
           LRHFGSS+STINSTN YFTGAS+AFAVF
Sbjct: 721 LRHFGSSRSTINSTNIYFTGASRAFAVF 748

BLAST of ClCG08G009620 vs. NCBI nr
Match: KAG7030054.1 (Neutral ceramidase 2, partial [Cucurbita argyrosperma subsp. argyrosperma])

HSP 1 Score: 1306.2 bits (3379), Expect = 0.0e+00
Identity = 657/765 (85.88%), Postives = 693/765 (90.59%), Query Frame = 0

Query: 1   MRWQLLK----RLMAMATVIVAVNGDWLVGVGSFDMTGPAAGVNMMGYANMDQNTAGIHF 60
           MR QLLK     ++A  +   A +GDWLVGVGSFDMTGPAA VNMMGYANMDQ+ AGIHF
Sbjct: 1   MRRQLLKVVATAVLAAVSCCCAADGDWLVGVGSFDMTGPAAEVNMMGYANMDQSAAGIHF 60

Query: 61  RLRARSFVVAKSVDGPRIAFVNLDAGMASQLVTIKVLERLKS---------------RFG 120
           RLRAR+F+VAKSVDGPRIAFVNLDAGMASQLVTIK+LERLK+               RFG
Sbjct: 61  RLRARTFIVAKSVDGPRIAFVNLDAGMASQLVTIKLLERLKASLTFLSKLKPRHFIQRFG 120

Query: 121 NLYTEENVAISGIHTHAGPGGYLQYLVYSITSLGFVQQSFDAIVDAIEQSIVQAHESLKP 180
           NLYTEENVAISGIHTHAGPGGYLQYLVYSITSLGFVQQSFDAIV+AIEQSIVQAH SLKP
Sbjct: 121 NLYTEENVAISGIHTHAGPGGYLQYLVYSITSLGFVQQSFDAIVNAIEQSIVQAHGSLKP 180

Query: 181 GFILFNKGDVANAGINRSPSAYLMNPQQERDRYATNVDKEMTVLKFVDGGSGKNIGAFSW 240
           GFIL NKG+V NAGINRSPSAYLMNP +ER RY TNVD EM+++K V+GGSG ++GAFSW
Sbjct: 181 GFILINKGEVENAGINRSPSAYLMNPPEERARYPTNVDTEMSLVKVVEGGSGVSLGAFSW 240

Query: 241 FPTHGTSMSRDNKLISGDNKGAAARFFEDWV-ASNHTTTTFSNHTSDIVELMKKAETIKA 300
           FPTHGTSMSRDNKLISGDNKGAAARFFEDWV ASN +T   S++ SDIVELMKKAETIKA
Sbjct: 241 FPTHGTSMSRDNKLISGDNKGAAARFFEDWVAASNRSTRATSSNKSDIVELMKKAETIKA 300

Query: 301 TGGKRCSKTSSQASKVRKNDGSLFVAAFCQSNVGDVTPNVLGAFCTDSGKPCDFNRSSCH 360
           TGGKRCSKTSSQ+SKVRKNDGSLFV AFCQSNVGDVTPNVLGAFCTDSGKPCDFNRSSCH
Sbjct: 301 TGGKRCSKTSSQSSKVRKNDGSLFVGAFCQSNVGDVTPNVLGAFCTDSGKPCDFNRSSCH 360

Query: 361 GSDLLCVGRGPGFPDEIRSTKIIGERQFQKAAELFTTATEKLTGEIDFRHVYLNFTDIQV 420
           GSDLLCVGRGPGFPDEI STKIIGERQF KA +LFTTATE+LTGEIDFRHVYLNFTDI+V
Sbjct: 361 GSDLLCVGRGPGFPDEILSTKIIGERQFLKAVDLFTTATERLTGEIDFRHVYLNFTDIEV 420

Query: 421 EIDGNNVVKTCPAALGPGFAAGTTDGPGVFGFQQGDTEINKLWKKLRDSLRKPSEFQVDC 480
           E+ G+NVVKTCPAALGPGFAAGTTDGPGVFGFQQGDT+INKLWKKLRDSLRKPSEFQV C
Sbjct: 421 ELGGDNVVKTCPAALGPGFAAGTTDGPGVFGFQQGDTQINKLWKKLRDSLRKPSEFQVGC 480

Query: 481 QKPKPVLLDTGEMFKPYAWAPAILPIQILRLGKLIILSVPGEFTTMAGRRLREAVKETLI 540
           QKPK VLLDTGEMF+PYAWAPAILPIQILRLGKLIILSVPGEFTTMAGRRLREAVKETLI
Sbjct: 481 QKPKTVLLDTGEMFEPYAWAPAILPIQILRLGKLIILSVPGEFTTMAGRRLREAVKETLI 540

Query: 541 SNGNGEFDDDTHVVIAGLTNAYSQYVATFEEYEQQRYEAASTLYGPHTLSAYIQEFKKLA 600
           SNGNGEFDDDTHVVIAGLTNAYSQYVAT EEYEQQRYEAASTLYGPHTLSAYIQEFKKLA
Sbjct: 541 SNGNGEFDDDTHVVIAGLTNAYSQYVATSEEYEQQRYEAASTLYGPHTLSAYIQEFKKLA 600

Query: 601 TAIAKGEKIVKPIASPPDLSSVQLRFVLDPFGESPPNGTNFGDIKQDVNLPENGWFKRGG 660
            AIAKGEKI KPI SPPDLSSVQLRFV DPF ESPPNG NFGDI+QD+ LP+ GWFKRG 
Sbjct: 601 IAIAKGEKIAKPIVSPPDLSSVQLRFVSDPFIESPPNGVNFGDIQQDIKLPKVGWFKRGS 660

Query: 661 IQKPTATFWSANPRFDLLTEGTYAVVEKLEKQRWTPVYDDDDISLFFKWTLD-NTFMNSL 720
            QKPTA FWSANPRFDLLTEGTYAVVE+LEK+RWTPVYDDDD SL FKW LD NTF NSL
Sbjct: 661 KQKPTARFWSANPRFDLLTEGTYAVVERLEKRRWTPVYDDDDFSLVFKWALDNNTFTNSL 720

Query: 721 VTIEWDIPINADPGVYRLRHFGSSKSTINSTNTYFTGASQAFAVF 745
            TIEWDIPI+A+PGVYRLRHFGSSKS INSTN YFTGAS AFAVF
Sbjct: 721 ATIEWDIPIDANPGVYRLRHFGSSKSAINSTNIYFTGASSAFAVF 765

BLAST of ClCG08G009620 vs. NCBI nr
Match: KAG6599114.1 (Neutral ceramidase 2, partial [Cucurbita argyrosperma subsp. sororia])

HSP 1 Score: 1305.0 bits (3376), Expect = 0.0e+00
Identity = 656/765 (85.75%), Postives = 693/765 (90.59%), Query Frame = 0

Query: 1   MRWQLLK----RLMAMATVIVAVNGDWLVGVGSFDMTGPAAGVNMMGYANMDQNTAGIHF 60
           MR QLLK     ++A  +   A +GDWLVGVGSFDMTGPAA VNMMGYANMDQ+ AGIHF
Sbjct: 1   MRRQLLKVVATAVLAAVSCCCAADGDWLVGVGSFDMTGPAAEVNMMGYANMDQSAAGIHF 60

Query: 61  RLRARSFVVAKSVDGPRIAFVNLDAGMASQLVTIKVLERLKS---------------RFG 120
           RLRAR+F+VAKSVDGPRIAFVNLDAGMASQLVTIK+LERLK+               RFG
Sbjct: 61  RLRARTFIVAKSVDGPRIAFVNLDAGMASQLVTIKLLERLKASLTYLSKLKPRHFIQRFG 120

Query: 121 NLYTEENVAISGIHTHAGPGGYLQYLVYSITSLGFVQQSFDAIVDAIEQSIVQAHESLKP 180
           NLYTEENVAISGIHTHAGPGGYLQYLVYSITSLGFVQQSFDAIV+AIEQSIVQAH SLKP
Sbjct: 121 NLYTEENVAISGIHTHAGPGGYLQYLVYSITSLGFVQQSFDAIVNAIEQSIVQAHGSLKP 180

Query: 181 GFILFNKGDVANAGINRSPSAYLMNPQQERDRYATNVDKEMTVLKFVDGGSGKNIGAFSW 240
           GFIL NKG+V NAGINRSPSAYLMNP +ER RY TNVD EM+++K V+GGSG ++GAFSW
Sbjct: 181 GFILINKGEVENAGINRSPSAYLMNPPEERARYPTNVDTEMSLVKVVEGGSGVSLGAFSW 240

Query: 241 FPTHGTSMSRDNKLISGDNKGAAARFFEDWV-ASNHTTTTFSNHTSDIVELMKKAETIKA 300
           FPTHGTSMSRDNKLISGDNKGAAARFFEDWV ASN +T   S++ SDI+ELMKKAETIKA
Sbjct: 241 FPTHGTSMSRDNKLISGDNKGAAARFFEDWVAASNRSTRATSSNKSDILELMKKAETIKA 300

Query: 301 TGGKRCSKTSSQASKVRKNDGSLFVAAFCQSNVGDVTPNVLGAFCTDSGKPCDFNRSSCH 360
           TGGKRCSKTSSQ+SKVRKNDGSLFV AFCQSNVGDVTPNVLGAFCTDSGKPCDFNRSSCH
Sbjct: 301 TGGKRCSKTSSQSSKVRKNDGSLFVGAFCQSNVGDVTPNVLGAFCTDSGKPCDFNRSSCH 360

Query: 361 GSDLLCVGRGPGFPDEIRSTKIIGERQFQKAAELFTTATEKLTGEIDFRHVYLNFTDIQV 420
           GSDLLCVGRGPGFPDEI STKIIGERQF KA +LFTTATE+LTGEIDFRHVYLNFTDI+V
Sbjct: 361 GSDLLCVGRGPGFPDEILSTKIIGERQFLKAVDLFTTATERLTGEIDFRHVYLNFTDIEV 420

Query: 421 EIDGNNVVKTCPAALGPGFAAGTTDGPGVFGFQQGDTEINKLWKKLRDSLRKPSEFQVDC 480
           E+ G+NVVKTCPAALGPGFAAGTTDGPGVFGFQQGDT+INKLWKKLRDSLRKPSEFQV C
Sbjct: 421 ELGGDNVVKTCPAALGPGFAAGTTDGPGVFGFQQGDTQINKLWKKLRDSLRKPSEFQVGC 480

Query: 481 QKPKPVLLDTGEMFKPYAWAPAILPIQILRLGKLIILSVPGEFTTMAGRRLREAVKETLI 540
           QKPK VLLDTGEMF+PYAWAPAILPIQILRLGKLIILSVPGEFTTMAGRRLREAVKETLI
Sbjct: 481 QKPKTVLLDTGEMFEPYAWAPAILPIQILRLGKLIILSVPGEFTTMAGRRLREAVKETLI 540

Query: 541 SNGNGEFDDDTHVVIAGLTNAYSQYVATFEEYEQQRYEAASTLYGPHTLSAYIQEFKKLA 600
           SNGNGEFDDDTHVVIAGLTNAYSQYVAT EEYEQQRYEAASTLYGPHTLSAYIQEFKKLA
Sbjct: 541 SNGNGEFDDDTHVVIAGLTNAYSQYVATSEEYEQQRYEAASTLYGPHTLSAYIQEFKKLA 600

Query: 601 TAIAKGEKIVKPIASPPDLSSVQLRFVLDPFGESPPNGTNFGDIKQDVNLPENGWFKRGG 660
            AIAKGEKI KPI SPPDLSSVQLRFV DPF ESPPNG NFGDI+QD+ LP+ GWFKRG 
Sbjct: 601 IAIAKGEKIAKPIVSPPDLSSVQLRFVSDPFIESPPNGVNFGDIQQDIKLPKVGWFKRGS 660

Query: 661 IQKPTATFWSANPRFDLLTEGTYAVVEKLEKQRWTPVYDDDDISLFFKWTLD-NTFMNSL 720
            QKPTA FWSANPRFDLLTEGTYAVVE+LEK+RWTPVYDDDD SL FKW LD NTF NSL
Sbjct: 661 KQKPTARFWSANPRFDLLTEGTYAVVERLEKRRWTPVYDDDDFSLVFKWALDNNTFTNSL 720

Query: 721 VTIEWDIPINADPGVYRLRHFGSSKSTINSTNTYFTGASQAFAVF 745
            TIEWDIPI+A+PGVYRLRHFGSSKS INSTN YFTGAS AFAVF
Sbjct: 721 ATIEWDIPIDANPGVYRLRHFGSSKSAINSTNIYFTGASSAFAVF 765

BLAST of ClCG08G009620 vs. ExPASy Swiss-Prot
Match: Q304B9 (Neutral ceramidase 2 OS=Arabidopsis thaliana OX=3702 GN=NCER2 PE=3 SV=1)

HSP 1 Score: 854.4 bits (2206), Expect = 9.8e-247
Identity = 443/739 (59.95%), Postives = 542/739 (73.34%), Query Frame = 0

Query: 23  WLVGVGSFDMTGPAAGVNMMGYANMDQNTAGIHFRLRARSFVVAKSVDGPRIAFVNLDAG 82
           +L+GVGS+D+TGPAA VNMMGYAN DQ  +GIHFRLRAR+F+VA+   G R+ FVNLDA 
Sbjct: 26  YLIGVGSYDITGPAADVNMMGYANSDQIASGIHFRLRARAFIVAEP-QGNRVVFVNLDAC 85

Query: 83  MASQLVTIKVLERLKSRFGNLYTEENVAISGIHTHAGPGGYLQYLVYSITSLGFVQQSFD 142
           MASQ+VTIKVLERLK+R+G LYTE+NVAISGIHTHAGPGGYLQY+ Y +TSLGFV+QSFD
Sbjct: 86  MASQIVTIKVLERLKARYGELYTEKNVAISGIHTHAGPGGYLQYVTYIVTSLGFVRQSFD 145

Query: 143 AIVDAIEQSIVQAHESLKPGFILFNKGDVANAGINRSPSAYLMNPQQERDRYATNVDKEM 202
            +V+ IEQSIVQAHESL+PG    NKGD+ +AG+NRSPS+YL NP  ER +Y  +VDKEM
Sbjct: 146 VVVNGIEQSIVQAHESLRPGSAFVNKGDLLDAGVNRSPSSYLNNPAAERSKYKYDVDKEM 205

Query: 203 TVLKFVDGGSGKNIGAFSWFPTHGTSMSRDNKLISGDNKGAAARFFEDW--------VAS 262
           T++KFVD   G   G+F+WF THGTSMSR N LISGDNKGAAARF EDW        V+S
Sbjct: 206 TLVKFVDSQLGPT-GSFNWFATHGTSMSRTNSLISGDNKGAAARFMEDWFENGQKNSVSS 265

Query: 263 NHTTTTFSNHTSDIV----ELMKKAETIKATGGKRCSKTSSQASKVRKNDGSLFVAAFCQ 322
            +     S   SD       L+  A T K++ G    K+    ++VR      FV+AFCQ
Sbjct: 266 RNIPRRVSTIVSDFSRNRDRLLDIAATYKSSRGHSVDKSLDVKTRVRNGSKRKFVSAFCQ 325

Query: 323 SNVGDVTPNVLGAFCTDSGKPCDFNRSSCHGSDLLCVGRGPGFPDEIRSTKIIGERQFQK 382
           SN GDV+PN LG FC D+G PCDFN S+C+G + LC GRGPG+PDE  ST+IIGE+QF+ 
Sbjct: 326 SNCGDVSPNTLGTFCIDTGLPCDFNHSTCNGQNELCYGRGPGYPDEFESTRIIGEKQFKM 385

Query: 383 AAELFTTATEKLTGEIDFRHVYLNFTDIQVEI----DGNNVVKTCPAALGPGFAAGTTDG 442
           A ELF  ATEKL G+I ++H YL+F+++ V +     G+  VKTCPAA+G GFAAGTTDG
Sbjct: 386 AVELFNKATEKLQGKIGYQHAYLDFSNLDVTVPKAGGGSETVKTCPAAMGFGFAAGTTDG 445

Query: 443 PGVFGFQQGDTEINKLWKKLRDSLRKPSEFQVDCQKPKPVLLDTGEMFKPYAWAPAILPI 502
           PG F F+QGD + N  W+ +R+ LR P   QV CQKPKP+LLDTGEM +PY WAP+ILPI
Sbjct: 446 PGAFDFKQGDDQGNVFWRLVRNVLRTPGPEQVQCQKPKPILLDTGEMKEPYDWAPSILPI 505

Query: 503 QILRLGKLIILSVPGEFTTMAGRRLREAVKETLISNGNGEFDDDTHVVIAGLTNAYSQYV 562
           QILR+G+L+ILSVPGEFTTMAGRRLR+A+K  LIS+   EF ++ HVVIAGLTN YSQY+
Sbjct: 506 QILRIGQLVILSVPGEFTTMAGRRLRDAIKSFLISSDPKEFSNNMHVVIAGLTNTYSQYI 565

Query: 563 ATFEEYEQQRYEAASTLYGPHTLSAYIQEFKKLATAIAKGEKIVKPIASPPDLSSVQLRF 622
           ATFEEYE QRYE ASTLYG HTL+AYIQEFKKLATA+  G  + +    PPDL   Q+  
Sbjct: 566 ATFEEYEVQRYEGASTLYGRHTLTAYIQEFKKLATALVNGLTLPRG-PQPPDLLDKQISL 625

Query: 623 VLDPFGESPPNGTNFGDIKQDVNLPENGWFKRGGIQKPTATFWSANPRFDLLTEGTYAVV 682
           +     +S P G  FGD+K DV  P    F+RG  Q+  ATFWS  PR DL+TEG++AVV
Sbjct: 626 LSPVVVDSTPLGVKFGDVKADV--PPKSTFRRG--QQVNATFWSGCPRNDLMTEGSFAVV 685

Query: 683 EKL-EKQRWTPVYDDDDISLFFKWTLDNTFMN-SLVTIEWDIPINADPGVYRLRHFGSSK 742
           E L E  +W PVYDDDD SL FKW+      + S  TIEW +P +A  GVYR+RH+G+SK
Sbjct: 686 ETLREGGKWAPVYDDDDFSLKFKWSRPAKLSSESQATIEWRVPESAVAGVYRIRHYGASK 745

Query: 743 STINSTNTYFTGASQAFAV 744
           S   S ++ F+G+S AF V
Sbjct: 746 SLFGSISS-FSGSSSAFVV 756

BLAST of ClCG08G009620 vs. ExPASy Swiss-Prot
Match: Q0JL46 (Neutral ceramidase OS=Oryza sativa subsp. japonica OX=39947 GN=Os01g0624000 PE=1 SV=1)

HSP 1 Score: 843.6 bits (2178), Expect = 1.7e-243
Identity = 435/773 (56.27%), Postives = 562/773 (72.70%), Query Frame = 0

Query: 3   WQLLKRLMAMATVIVAVNGDWLVGVGSFDMTGPAAGVNMMGYANMDQNTAGIHFRLRARS 62
           W LL  ++   +++++ +  +LVG+GSFD+TGPAA VNMMGYAN +Q  +GIHFRL++R+
Sbjct: 22  WLLLALVLLNCSLVLSAS-PYLVGMGSFDITGPAADVNMMGYANTEQIASGIHFRLKSRA 81

Query: 63  FVVAKSVDGPRIAFVNLDAGMASQLVTIKVLERLKSRFGNLYTEENVAISGIHTHAGPGG 122
           F+VA+  +G R+ FVN+DA MASQ+VTIKVLERLK+R+G+LY E NVAISGIHTHAGPGG
Sbjct: 82  FIVAEP-NGKRVVFVNIDACMASQIVTIKVLERLKARYGDLYNENNVAISGIHTHAGPGG 141

Query: 123 YLQYLVYSITSLGFVQQSFDAIVDAIEQSIVQAHESLKPGFILFNKGDVANAGINRSPSA 182
           YLQY+VY +TSLGFV+QSFD IVD IEQSIV+AH +L+PG I  NKGD+ +AG+NRSPSA
Sbjct: 142 YLQYVVYIVTSLGFVRQSFDVIVDGIEQSIVEAHNNLRPGKIFVNKGDLLDAGVNRSPSA 201

Query: 183 YLMNPQQERDRYATNVDKEMTVLKFVDGGSGKNIGAFSWFPTHGTSMSRDNKLISGDNKG 242
           YL NP +ER +Y  NVDKEMT++KFVD   G  +G+F+WF THGTSMSR N LISGDNKG
Sbjct: 202 YLNNPAEERSKYEYNVDKEMTLIKFVDDELGP-VGSFNWFATHGTSMSRTNSLISGDNKG 261

Query: 243 AAARFFEDW-----------------VASNHTTTTFSNHTSDIV--------ELMKKAET 302
           AAARF EDW                 + S H T+      S I+        +L++ A +
Sbjct: 262 AAARFMEDWAEQMGLPKQSAHANSDDLRSLHKTSVLPRRVSTIIPEPNEITDDLIQLASS 321

Query: 303 IKATGGKRCSKTS-SQASKVRKNDGSLFVAAFCQSNVGDVTPNVLGAFCTDSGKPCDFNR 362
            +A+GG+R + +S ++  +  + +   FV+AFCQSN GDV+PNVLG FC D+  PCDFN 
Sbjct: 322 YEASGGRRLAGSSITRRIRSTQQNKPKFVSAFCQSNCGDVSPNVLGTFCIDTNLPCDFNH 381

Query: 363 SSCHGSDLLCVGRGPGFPDEIRSTKIIGERQFQKAAELFTTATEKLTGEIDFRHVYLNFT 422
           S+C+G + LC GRGPG+PDE  ST++IG RQF KA +LF +A+E++ G+ID+RH YL+F+
Sbjct: 382 STCNGKNELCYGRGPGYPDEFESTRVIGNRQFLKARDLFDSASEEIQGKIDYRHTYLDFS 441

Query: 423 DIQVEID----GNNVVKTCPAALGPGFAAGTTDGPGVFGFQQGDTEINKLWKKLRDSLRK 482
            ++V++     G   VKTCPAA+G  FAAGTTDGPG F F+QGD + N  WK +R+ L+ 
Sbjct: 442 KLEVKVSTSAGGQQTVKTCPAAMGFAFAAGTTDGPGAFDFRQGDVKGNPFWKLVRNLLKT 501

Query: 483 PSEFQVDCQKPKPVLLDTGEMFKPYAWAPAILPIQILRLGKLIILSVPGEFTTMAGRRLR 542
           P + QV+C  PKP+LLDTGEM +PY WAPAILP+Q++R+G+L+IL VPGEFTTMAGRRLR
Sbjct: 502 PGKDQVECHSPKPILLDTGEMKEPYDWAPAILPVQMIRIGQLVILCVPGEFTTMAGRRLR 561

Query: 543 EAVKETLISNGNGEFDDDTHVVIAGLTNAYSQYVATFEEYEQQRYEAASTLYGPHTLSAY 602
           +AVK T++++GN EFD + HVV+AGLTN+YSQY+ TFEEY+ QRYE ASTLYGPHTLSAY
Sbjct: 562 DAVK-TVLTSGNSEFDKNIHVVLAGLTNSYSQYITTFEEYQIQRYEGASTLYGPHTLSAY 621

Query: 603 IQEFKKLATAIAKGEKIVKPIASPPDLSSVQLRFVLDPFGESPPNGTNFGDIKQDVNLPE 662
           IQEF+KLA A+    K V     PPD+   Q+  +     +S P G  FGD+  DV  P 
Sbjct: 622 IQEFQKLAMAMI-ANKEVPTNFQPPDMLDKQIGLLPGVVFDSTPLGVKFGDVNSDV--PG 681

Query: 663 NGWFKRGGIQKPTATFWSANPRFDLLTEGTYAVVEKLE-KQRWTPVYDDDDISLFFKWTL 722
           N  F +G      ATF+SA PR DLLT+GT+A+VEKL+    W PVYDDDD SL FKW+ 
Sbjct: 682 NSTFNKG--STVNATFYSACPRNDLLTDGTFALVEKLDGNNNWVPVYDDDDWSLRFKWSR 741

Query: 723 DNTFMN-SLVTIEWDIPINADPGVYRLRHFGSSKSTINSTNTYFTGASQAFAV 744
                + S  T+EW +P +A  GVYRLRHFG+SK    S   +FTG S+AFAV
Sbjct: 742 PARLSSRSFATLEWTVPEDAAAGVYRLRHFGASKPMFGSVR-HFTGTSRAFAV 784

BLAST of ClCG08G009620 vs. ExPASy Swiss-Prot
Match: F4HQM3 (Neutral ceramidase 1 OS=Arabidopsis thaliana OX=3702 GN=NCER1 PE=2 SV=1)

HSP 1 Score: 841.3 bits (2172), Expect = 8.6e-243
Identity = 432/752 (57.45%), Postives = 549/752 (73.01%), Query Frame = 0

Query: 20  NGDWLVGVGSFDMTGPAAGVNMMGYANMDQNTAGIHFRLRARSFVVAKSVDGPRIAFVNL 79
           + ++L+G+GS+D+TGPAA VNMMGYANM+Q  +GIHFRLRAR+F+V++   G R+ FVNL
Sbjct: 34  HSEYLIGLGSYDITGPAADVNMMGYANMEQVASGIHFRLRARTFIVSEP-QGKRVVFVNL 93

Query: 80  DAGMASQLVTIKVLERLKSRFGNLYTEENVAISGIHTHAGPGGYLQYLVYSITSLGFVQQ 139
           DA MASQ+V +KV+ERLK+R+G+LYTE+NV ISGIHTHAGPGGYLQY+VY +TSLGFV+Q
Sbjct: 94  DACMASQIVKLKVIERLKARYGDLYTEQNVGISGIHTHAGPGGYLQYVVYIVTSLGFVRQ 153

Query: 140 SFDAIVDAIEQSIVQAHESLKPGFILFNKGDVANAGINRSPSAYLMNPQQERDRYATNVD 199
           SFDA+VD IE SI+QAHE+L+PG I  N G++ +AG+NRSPSAYL NP +ER ++  NVD
Sbjct: 154 SFDALVDGIENSIIQAHENLRPGSIFLNNGELLDAGVNRSPSAYLNNPSKERSKHKYNVD 213

Query: 200 KEMTVLKFVDGGSGKNIGAFSWFPTHGTSMSRDNKLISGDNKGAAARFFEDWVASNHTTT 259
           KEMT+LKFVD   G  +G+F+WF THGTSMSR N LISGDNKGAA+RF EDW   N    
Sbjct: 214 KEMTLLKFVDDQWGP-VGSFNWFATHGTSMSRTNSLISGDNKGAASRFMEDWYEQNTAER 273

Query: 260 TFS-----------------NHTSDIVELMKKAETIKATGGKRCSKTSSQASKV----RK 319
           ++S                 NH     EL++ A   ++  GK  ++ SS A +V    RK
Sbjct: 274 SYSEEFISDEIPRRVSSLIENHQDSHHELLELASYFESQPGKPVTRISSSARRVRSALRK 333

Query: 320 NDGSLFVAAFCQSNVGDVTPNVLGAFCTDSGKPCDFNRSSCHGSDLLCVGRGPGFPDEIR 379
            D   FV+AFCQ+N GDV+PNVLGAFC D+G PCDFN S+C G + +C GRGPG+PDE  
Sbjct: 334 ADKPGFVSAFCQTNCGDVSPNVLGAFCLDTGLPCDFNHSTCGGKNEMCYGRGPGYPDEFE 393

Query: 380 STKIIGERQFQKAAELFTTATEKLTGEIDFRHVYLNFTDIQVEI---DG-NNVVKTCPAA 439
           ST+IIGERQF+ A ELF  A+E+L G++D+RHVY++F+ + V +   DG + VVKTCPAA
Sbjct: 394 STRIIGERQFKMALELFNKASEQLQGKVDYRHVYVDFSQLNVTLPKKDGKSEVVKTCPAA 453

Query: 440 LGPGFAAGTTDGPGVFGFQQGDTEINKLWKKLRDSLRKPSEFQVDCQKPKPVLLDTGEMF 499
           +G  FAAGTTDGPG F F QGD + N  W+ +R+ L+ P + Q+DC  PKP+LLDTGEM 
Sbjct: 454 MGFAFAAGTTDGPGAFDFTQGDDKGNPFWRLVRNVLKTPDKKQIDCHYPKPILLDTGEMT 513

Query: 500 KPYAWAPAILPIQILRLGKLIILSVPGEFTTMAGRRLREAVKETLISNGNGEFDDDTHVV 559
           KPY WAP+IL +Q+LR+G+L ILSVPGEFTTMAGRRLR AVK  L ++GN +   + HVV
Sbjct: 514 KPYDWAPSILSLQVLRIGQLFILSVPGEFTTMAGRRLRYAVKTQLKNSGNKDLSGEIHVV 573

Query: 560 IAGLTNAYSQYVATFEEYEQQRYEAASTLYGPHTLSAYIQEFKKLATAIAKGEKIVKPIA 619
           IAGL N YSQYV TFEEY+ QRYE ASTLYGPHTLS YIQEFKKL+ ++   +  V+P  
Sbjct: 574 IAGLANGYSQYVTTFEEYQVQRYEGASTLYGPHTLSGYIQEFKKLSKSLVL-DMPVQPGP 633

Query: 620 SPPDLSSVQLRFVLDPFGESPPNGTNFGDIKQDVNLPENGWFKRGGIQKPTATFWSANPR 679
            PPDL   QL F+     ++ P+G +FGD+  DV  P+N   KRG  Q  T  F SA PR
Sbjct: 634 QPPDLLDKQLSFLTPVMMDTTPSGDSFGDVISDV--PKNLSLKRGNGQ-VTVVFRSACPR 693

Query: 680 FDLLTEGTYAVVEKLEK--QRWTPVYDDDDISLFFKWTLDNTFMN-SLVTIEWDIPINAD 739
            DLLTEGT+ +VE+LE+  + WTPVYDDDD+ L FKW+      + S  T+EW IP +A 
Sbjct: 694 NDLLTEGTFTLVERLEQKDKTWTPVYDDDDLCLRFKWSRHKKLSSRSQATVEWRIPESAS 753

Query: 740 PGVYRLRHFGSSKSTINSTNTYFTGASQAFAV 744
           PGVYR+ HFG++K    S + +FTG+S AF V
Sbjct: 754 PGVYRITHFGAAKKLFGSVH-HFTGSSSAFVV 778

BLAST of ClCG08G009620 vs. ExPASy Swiss-Prot
Match: F4KHQ8 (Neutral ceramidase 3 OS=Arabidopsis thaliana OX=3702 GN=NCER3 PE=2 SV=1)

HSP 1 Score: 790.0 bits (2039), Expect = 2.3e-227
Identity = 420/769 (54.62%), Postives = 533/769 (69.31%), Query Frame = 0

Query: 2   RWQL-----LKRLMAMATVIVAVNGDWLVGVGSFDMTGPAAGVNMMGYANMDQNTAGIHF 61
           RW +     L  L  +    +  + D+L+G+GS+D+TGPAA VNMMGYANM+Q  +G+HF
Sbjct: 3   RWSMSMHCTLFLLFLLRLTCIFSDSDYLMGLGSYDITGPAADVNMMGYANMEQVASGVHF 62

Query: 62  RLRARSFVVAKSVDGPRIAFVNLDAGMASQLVTIKVLERLKSRFGNLYTEENVAISGIHT 121
           RLRAR+F+VA+     RIAFVNLDAGMASQLVTIKV+ERLK R+G LYTEENVAISG HT
Sbjct: 63  RLRARAFIVAEPYK-KRIAFVNLDAGMASQLVTIKVIERLKQRYGELYTEENVAISGTHT 122

Query: 122 HAGPGGYLQYLVYSITSLGFVQQSFDAIVDAIEQSIVQAHESLKPGFILFNKGDVANAGI 181
           HAGPGGYLQY++Y +TSLGFV QSF+A+VD IEQSI+QAHE+L+PG IL NKG++ +AG+
Sbjct: 123 HAGPGGYLQYILYLVTSLGFVHQSFNALVDGIEQSIIQAHENLRPGSILINKGELLDAGV 182

Query: 182 NRSPSAYLMNPQQERDRYATNVDKEMTVLKFVDGGSGKNIGAFSWFPTHGTSMSRDNKLI 241
           NRSPSAYL NP  ER +Y  +VDKEMT++KFVD                           
Sbjct: 183 NRSPSAYLNNPAHERSKYEYDVDKEMTLVKFVD--------------------------- 242

Query: 242 SGDNKGAAARFFEDWVASNHTTTTFS----NHTSDIV------ELMKKAETIKATGGKRC 301
             D  G  AR  EDW    +   +         S I+      +LM+ A ++ +TGGK  
Sbjct: 243 --DQWGPVARIMEDWFERENGCRSVDVESPRRVSSIISDPYDQDLMEMASSLLSTGGKTV 302

Query: 302 SKTSSQASKVRKN----DGSLFVAAFCQSNVGDVTPNVLGAFCTDSGKPCDFNRSSCHGS 361
           ++ SS A +VR      D   FV+AFCQ+N GDV+PNVLGAFC D+G PC+FN+S+C G 
Sbjct: 303 TRMSSVARRVRSRFRHADKPRFVSAFCQTNCGDVSPNVLGAFCIDTGLPCEFNQSTCGGK 362

Query: 362 DLLCVGRGPGFPDEIRSTKIIGERQFQKAAELFTTATEKLTGEIDFRHVYLNFTDIQVEI 421
           +  C GRGPG+PDE  ST+IIGERQF+KAA+LFT A+E++ G++D+RH Y++F+ ++V I
Sbjct: 363 NEQCYGRGPGYPDEFESTRIIGERQFKKAADLFTKASEEIQGKVDYRHAYVDFSQLEVTI 422

Query: 422 DGNN----VVKTCPAALGPGFAAGTTDGPGVFGFQQGDTEINKLWKKLRDSLRKPSEFQV 481
           +G N    VVKTCPAA+G GFAAGTTDGPG F F+QGD + N  W+ +R+ L+ P+E QV
Sbjct: 423 NGQNGGSEVVKTCPAAMGFGFAAGTTDGPGAFDFKQGDDQGNPFWRLVRNLLKNPTEEQV 482

Query: 482 DCQKPKPVLLDTGEMFKPYAWAPAILPIQILRLGKLIILSVPGEFTTMAGRRLREAVKET 541
            CQ+PKP+LLDTGEM +PY WAP+ILP+QILR+G+L+IL VPGEFTTMAGRRLR+AVK  
Sbjct: 483 RCQRPKPILLDTGEMKQPYDWAPSILPVQILRIGQLVILCVPGEFTTMAGRRLRDAVKTV 542

Query: 542 LISNGNGEFDDDTHVVIAGLTNAYSQYVATFEEYEQQRYEAASTLYGPHTLSAYIQEFKK 601
           L    NG    +  VVIAGLTN+YSQY+ATFEEY+ QRYE ASTLYGPHTLS YIQEFKK
Sbjct: 543 LKEGSNGR---EFSVVIAGLTNSYSQYIATFEEYQVQRYEGASTLYGPHTLSGYIQEFKK 602

Query: 602 LATAIAKGEKIVKPIASPPDLSSVQLRFVLDPFGESPPNGTNFGDIKQDVNLPENGWFKR 661
           LA  +   +    P   PPDL   Q+  +     +  P GT FGD+  DV  P    F++
Sbjct: 603 LANDLLSAQ-TTDPGPQPPDLLHKQISLLTPVVADMTPIGTAFGDVTSDV--PRLSKFRK 662

Query: 662 GGIQKPTATFWSANPRFDLLTEGTYAVVEK-LE-KQRWTPVYDDDDISLFFKWTLD-NTF 721
           G        F SANPR DL+TEGT+A+VE+ LE ++ W PVYDDDD  L FKW+      
Sbjct: 663 GA-DIVRVQFRSANPRNDLMTEGTFALVERWLEGRETWVPVYDDDDFCLRFKWSRPFKLS 722

Query: 722 MNSLVTIEWDIPINADPGVYRLRHFGSSKSTINSTNTYFTGASQAFAVF 745
             S  TIEW IP  A PGVYR+ HFGS+K+ I+S + +F+G+S AF V+
Sbjct: 723 TQSTATIEWRIPETASPGVYRITHFGSAKTPISSIH-HFSGSSSAFVVY 733

BLAST of ClCG08G009620 vs. ExPASy Swiss-Prot
Match: Q55G11 (Neutral ceramidase B OS=Dictyostelium discoideum OX=44689 GN=dcd2B PE=3 SV=1)

HSP 1 Score: 532.3 bits (1370), Expect = 8.6e-150
Identity = 313/733 (42.70%), Postives = 432/733 (58.94%), Query Frame = 0

Query: 20  NGDWLVGVGSFDMTGPAAGVNMMGYANMDQNTAGIHFRLRARSFVVAKSVDGPRIAFVNL 79
           +  + +G G +D+TG +A VN+MGYAN  Q  AGIHFR RAR+FV   S +G R  +V+ 
Sbjct: 43  DSSYQIGAGIYDITGASAEVNLMGYANPLQVGAGIHFRQRARAFVFVDS-NGNRAVYVST 102

Query: 80  DAGMASQLVTIKVLERLKSRFG-NLYTEENVAISGIHTHAGPGGYLQYLVYSITSLGFVQ 139
           D+ M  Q V I V+E L+  FG N+YTE NV +SG HTH+GP G+ QY +Y ITSLGF +
Sbjct: 103 DSCMIFQEVKIHVVELLQDIFGPNVYTEANVLLSGTHTHSGPAGFSQYALYGITSLGFYK 162

Query: 140 QSFDAIVDAIEQSIVQAHESLKPGFILFNKGDVANAGINRSPSAYLMNPQQERDRYATNV 199
           ++FD I + I Q+IV+AH+S++P  +    G++ N  INRSP AY  NP++E+  Y +NV
Sbjct: 163 KNFDTICNGIVQAIVKAHKSVQPANMFTETGELWNTNINRSPFAYDNNPEEEKAMYDSNV 222

Query: 200 DKEMTVLKFVDGGSGKNIGAFSWFPTHGTSMSRDNKLISGDNKGAAARFFEDWVASNHTT 259
           DK MTVL+ ++  +G    A S+F  H TSM+  N LISGDNKG A+  +E  V      
Sbjct: 223 DKNMTVLR-IEDMNGNPFAAISFFAVHCTSMNNTNHLISGDNKGYASYLWEKQVNG---- 282

Query: 260 TTFSNHTSDIVELMKKAETIKATGGKRCSKTSSQASKVRKNDGSLFVAAFCQSNVGDVTP 319
                                 T GK                   FVAAF QSN GDV+P
Sbjct: 283 --------------------PGTAGK-----------------GPFVAAFGQSNEGDVSP 342

Query: 320 NVLGAFCTDSGKPCDFNRSSCHGSDLLCVGRGPGFP-DEIRSTKIIGERQFQKAAELFTT 379
           N  G  C D G PCD+  S+C+G +  C   GPG   D   ST+IIG  QF KA ELF  
Sbjct: 343 NTRGPTCRD-GSPCDYKTSTCNGRNEECWSLGPGKDGDMFESTQIIGGNQFNKALELFNN 402

Query: 380 ATEKLTGEIDFRHVYLNFTDIQVEIDGNNVV---KTCPAALGPGFAAGTTDGPGVFGFQQ 439
           A+ +++G + +RH ++ FT++ VE   N+ V    TC  A+G  FAAGTTDGPG F F Q
Sbjct: 403 ASIQVSGPVQYRHSWVQFTNVSVEPPYNSGVDNATTCRGAMGYSFAAGTTDGPGAFNFVQ 462

Query: 440 GD--TEINKLWKKLRDSLRKPSEFQVDCQKPKPVLLDTGEMFKPYAWAPAILPIQILRLG 499
            D  T  N  W  + D + KP+  Q+ CQ PKP+LLD G M +P  W P ++PIQI+ +G
Sbjct: 463 SDNNTSGNPFWNFIGDFIAKPTPDQIRCQSPKPILLDVG-MVEPIPWVPDVMPIQIVTIG 522

Query: 500 KLIILSVPGEFTTMAGRRLREAVKETLISNGNGEFDDDTHVVIAGLTNAYSQYVATFEEY 559
           ++++++VPGEFTTM+GRRLR +V+E +     GE  ++  V+IAGL+N YS Y+ATFEEY
Sbjct: 523 QIVLVAVPGEFTTMSGRRLRNSVREII-----GESIENPIVLIAGLSNTYSGYIATFEEY 582

Query: 560 EQQRYEAASTLYGPHTLSAYIQEFKKLATAIAKGEKIVKPIASPPDLSSVQLRFVLDPFG 619
           + QRYE AST++GPHTL +Y+QEF KLA +I  G   V    +P +L+   L F+     
Sbjct: 583 QVQRYEGASTVFGPHTLGSYMQEFGKLAQSIVDG-TTVPAGPTPRNLTGHTLFFLPPVIV 642

Query: 620 ESPPNGTNFGDIKQDVNLPENGWFKRGGIQKPTATFWSANPRFDLLTEGTYAVVEKLE-K 679
           ++ P+  +FG++  DVNL           +  +  F+  NPR D + E ++  V+ L   
Sbjct: 643 DAAPDFDDFGEVSIDVNL------NYSVNETVSCVFYGGNPRNDFMIESSFLSVDLLTGT 702

Query: 680 QRWTPVYDDDDISLFFKWTLDNTFMNSLVTIEWDIPINADPGVYRLRHFGSSKSTINSTN 739
            +WT V DD D    FKW + +    SL+TIEW I  +  PG YR+ H G +K    S+N
Sbjct: 703 DQWTTVLDDGDWDTKFKWKMHDLGF-SLITIEWVIAPDTTPGTYRITHSGFAKKNPFSSN 717

Query: 740 -TYFTGASQAFAV 744
            T + G S+ F V
Sbjct: 763 LTPYQGISRNFVV 717

BLAST of ClCG08G009620 vs. ExPASy TrEMBL
Match: A0A1S3CMI6 (Neutral ceramidase OS=Cucumis melo OX=3656 GN=LOC103502652 PE=3 SV=1)

HSP 1 Score: 1320.4 bits (3416), Expect = 0.0e+00
Identity = 657/748 (87.83%), Postives = 697/748 (93.18%), Query Frame = 0

Query: 12  MATVIV----AVNGDWLVGVGSFDMTGPAAGVNMMGYANMDQNTAGIHFRLRARSFVVAK 71
           MA V++     VNGDWLVGVGSFDMTGPAA VNMMGYANMDQ TAGIHFRLRAR+FVVA+
Sbjct: 1   MAIVVLDVSWCVNGDWLVGVGSFDMTGPAAEVNMMGYANMDQVTAGIHFRLRARTFVVAR 60

Query: 72  SVDGPRIAFVNLDAGMASQLVTIKVLERLKS---------RFGNLYTEENVAISGIHTHA 131
           S+DGPRIAFVNLDAGMASQLVTIKVLERL+S         RFG+LYTEENVAISGIHTHA
Sbjct: 61  SIDGPRIAFVNLDAGMASQLVTIKVLERLQSRRNINISVQRFGDLYTEENVAISGIHTHA 120

Query: 132 GPGGYLQYLVYSITSLGFVQQSFDAIVDAIEQSIVQAHESLKPGFILFNKGDVANAGINR 191
           GPGGYLQYLVYSITSLGFVQQSFDAIV+AIEQSI+QAHESLKPGFILFNKG+V NAGINR
Sbjct: 121 GPGGYLQYLVYSITSLGFVQQSFDAIVNAIEQSIIQAHESLKPGFILFNKGEVDNAGINR 180

Query: 192 SPSAYLMNPQQERDRYATNVDKEMTVLKFVDGGSGKNIGAFSWFPTHGTSMSRDNKLISG 251
           SPSAYLMNP++ER +YA NVDKEM++LKFVDG SG +IGAFSWFPTHGTSMSRDNKLISG
Sbjct: 181 SPSAYLMNPEEERAKYANNVDKEMSILKFVDGESGNSIGAFSWFPTHGTSMSRDNKLISG 240

Query: 252 DNKGAAARFFEDWVASN-HTTTTFSNHTSDIVELMKKAETIKATGGKRCSKTSSQASKVR 311
           DNKGAAARFFEDW ASN  TTTT SN+ S I ELMKKAE IKATGGK+CSKTSS++SKVR
Sbjct: 241 DNKGAAARFFEDWAASNGRTTTTPSNNASVIEELMKKAEKIKATGGKQCSKTSSKSSKVR 300

Query: 312 KNDGSLFVAAFCQSNVGDVTPNVLGAFCTDSGKPCDFNRSSCHGSDLLCVGRGPGFPDEI 371
           KNDGSLFVAAFCQSNVGDVTPNVLGAFC DSGKPCDFNRSSCHGSDLLCVGRGPGFPDEI
Sbjct: 301 KNDGSLFVAAFCQSNVGDVTPNVLGAFCIDSGKPCDFNRSSCHGSDLLCVGRGPGFPDEI 360

Query: 372 RSTKIIGERQFQKAAELFTTATEKLTGEIDFRHVYLNFTDIQVEIDGNNVVKTCPAALGP 431
            STKIIGERQF KAA+LFTTATEKLTGEIDFRHVYLNFTDI+VEIDGNNVV+TCPAALGP
Sbjct: 361 LSTKIIGERQFLKAADLFTTATEKLTGEIDFRHVYLNFTDIEVEIDGNNVVRTCPAALGP 420

Query: 432 GFAAGTTDGPGVFGFQQGDTEINKLWKKLRDSLRKPSEFQVDCQKPKPVLLDTGEMFKPY 491
           GFAAGTTDGPGVFGFQQGDTEINKLWK+LRDSL+KPSEFQV CQKPK VLLDTGEMF+PY
Sbjct: 421 GFAAGTTDGPGVFGFQQGDTEINKLWKQLRDSLKKPSEFQVGCQKPKTVLLDTGEMFEPY 480

Query: 492 AWAPAILPIQILRLGKLIILSVPGEFTTMAGRRLREAVKETLISNGNGEFDDDTHVVIAG 551
           AWAPAILPIQILRLGKLIILSVPGEFTTMAGRRLREAVKETLISNGNGEFDDDTHVVIAG
Sbjct: 481 AWAPAILPIQILRLGKLIILSVPGEFTTMAGRRLREAVKETLISNGNGEFDDDTHVVIAG 540

Query: 552 LTNAYSQYVATFEEYEQQRYEAASTLYGPHTLSAYIQEFKKLATAIAKGEKIVKPIASPP 611
           LTN YSQYVATFEEYEQQRYEAASTLYGPHTLSAYIQEFKKLA+AIAKGEK+ KPIASPP
Sbjct: 541 LTNTYSQYVATFEEYEQQRYEAASTLYGPHTLSAYIQEFKKLASAIAKGEKVAKPIASPP 600

Query: 612 DLSSVQLRFVLDPFGESPPNGTNFGDIKQDVNLPENGWFKRGGIQKPTATFWSANPRFDL 671
           DLSSVQLR V+DPFGESPP+G NFGDI+QDV LP++GWFK G  QKPTATFWSANPRFDL
Sbjct: 601 DLSSVQLRLVMDPFGESPPDGVNFGDIQQDVELPKDGWFKTGSKQKPTATFWSANPRFDL 660

Query: 672 LTEGTYAVVEKLEKQRWTPVYDDDDISLFFKWTLDN-TFMNSLVTIEWDIPINADPGVYR 731
           LTEGTYA+VE+LEKQRWTP YDDDD S+ FKW LDN T +NSL TIEWDIPI+ADPGVYR
Sbjct: 661 LTEGTYALVERLEKQRWTPAYDDDDFSVSFKWKLDNTTLINSLATIEWDIPIDADPGVYR 720

Query: 732 LRHFGSSKSTINSTNTYFTGASQAFAVF 745
           LRHFGSS+STINSTN YFTGAS+AFAVF
Sbjct: 721 LRHFGSSRSTINSTNIYFTGASRAFAVF 748

BLAST of ClCG08G009620 vs. ExPASy TrEMBL
Match: A0A0A0KII3 (Neutral ceramidase OS=Cucumis sativus OX=3659 GN=Csa_6G361230 PE=3 SV=1)

HSP 1 Score: 1173.3 bits (3034), Expect = 0.0e+00
Identity = 576/656 (87.80%), Postives = 609/656 (92.84%), Query Frame = 0

Query: 100 FGNLYTEENVAISGIHTHAGPGGYLQYLVYSITSLGFVQQSFDAIVDAIEQSIVQAHESL 159
           FG++YTEENVAISGIHTHAGPGGYLQYLVYSITSLGFVQQSFDAIV+AIEQSI+QAHESL
Sbjct: 4   FGDVYTEENVAISGIHTHAGPGGYLQYLVYSITSLGFVQQSFDAIVNAIEQSIIQAHESL 63

Query: 160 KPGFILFNKG----------DVANAGINRSPSAYLMNPQQERDRYATNVDKEMTVLKFVD 219
           KPGFILFNKG          +V NAGINRSPSAYLMNP++ER +YA NVDKEM++LKFVD
Sbjct: 64  KPGFILFNKGKQIRQLFHFWEVENAGINRSPSAYLMNPEEERAKYANNVDKEMSILKFVD 123

Query: 220 GGSGKNIGAFSWFPTHGTSMSRDNKLISGDNKGAAARFFEDWVASN-HTTTTFSNHTSDI 279
           G +G +IGAFSWFPTHGTSMSRDNKLISGDNKGAAARFFEDW ASN  TTTT SN+TS I
Sbjct: 124 GENGNSIGAFSWFPTHGTSMSRDNKLISGDNKGAAARFFEDWAASNRRTTTTPSNNTSGI 183

Query: 280 VELMKKAETIKATGGKRCSKTSSQASKVRKNDGSLFVAAFCQSNVGDVTPNVLGAFCTDS 339
            +LMKKA  IKATGGKRCSKTSS++SKVRKNDGSLFV AFCQSNVGDVTPNVLGAFCTDS
Sbjct: 184 EDLMKKAGKIKATGGKRCSKTSSKSSKVRKNDGSLFVGAFCQSNVGDVTPNVLGAFCTDS 243

Query: 340 GKPCDFNRSSCHGSDLLCVGRGPGFPDEIRSTKIIGERQFQKAAELFTTATEKLTGEIDF 399
           G PCDFN SSCHGSDLLCVGRGPGFPDEI STKIIGERQF KAA+LFTTATEKLTGEIDF
Sbjct: 244 GNPCDFNHSSCHGSDLLCVGRGPGFPDEILSTKIIGERQFLKAADLFTTATEKLTGEIDF 303

Query: 400 RHVYLNFTDIQVEIDGNNVVKTCPAALGPGFAAGTTDGPGVFGFQQGDTEINKLWKKLRD 459
           RHVYLNFTDI+V IDGNNVV+TCPAALGPGFAAGTTDGPG FGFQQGDTEINKLWK+LRD
Sbjct: 304 RHVYLNFTDIEVAIDGNNVVRTCPAALGPGFAAGTTDGPGAFGFQQGDTEINKLWKQLRD 363

Query: 460 SLRKPSEFQVDCQKPKPVLLDTGEMFKPYAWAPAILPIQILRLGKLIILSVPGEFTTMAG 519
           SL+KPSEFQV CQKPK VLLDTGEMF+PYAWAPAILPIQILRLG LIILSVPGEFTTMAG
Sbjct: 364 SLKKPSEFQVGCQKPKTVLLDTGEMFEPYAWAPAILPIQILRLGNLIILSVPGEFTTMAG 423

Query: 520 RRLREAVKETLISNGNGEFDDDTHVVIAGLTNAYSQYVATFEEYEQQRYEAASTLYGPHT 579
           RRLREAVKETLISNGNG FDDDTHVVIAGLTN YSQYVATFEEYEQQRYEAASTLYGPHT
Sbjct: 424 RRLREAVKETLISNGNGVFDDDTHVVIAGLTNTYSQYVATFEEYEQQRYEAASTLYGPHT 483

Query: 580 LSAYIQEFKKLATAIAKGEKIVKPIASPPDLSSVQLRFVLDPFGESPPNGTNFGDIKQDV 639
           LSAYIQEFKKLA AIAKGEKI KPIASPPDLSSVQLR V+DPFGESPPNG NFGDI+QDV
Sbjct: 484 LSAYIQEFKKLAIAIAKGEKIAKPIASPPDLSSVQLRLVMDPFGESPPNGVNFGDIQQDV 543

Query: 640 NLPENGWFKRGGIQKPTATFWSANPRFDLLTEGTYAVVEKLEKQRWTPVYDDDDISLFFK 699
            LP++GWFK G  QKPTATFWSANPRFDLLTEGTYA+VE+LEKQRWTP YDDDD SLFFK
Sbjct: 544 ELPKDGWFKTGSKQKPTATFWSANPRFDLLTEGTYALVERLEKQRWTPAYDDDDFSLFFK 603

Query: 700 WTLDNTFMNSLVTIEWDIPINADPGVYRLRHFGSSKSTINSTNTYFTGASQAFAVF 745
           W LDNTF+N+LVTIEWDIPI+ADPGVYRLRHFGSS+STINSTN YFTGAS+AFAVF
Sbjct: 604 WKLDNTFINNLVTIEWDIPIDADPGVYRLRHFGSSRSTINSTNVYFTGASRAFAVF 659

BLAST of ClCG08G009620 vs. ExPASy TrEMBL
Match: A0A2I4G350 (Neutral ceramidase OS=Juglans regia OX=51240 GN=LOC109004273 PE=3 SV=1)

HSP 1 Score: 1170.2 bits (3026), Expect = 0.0e+00
Identity = 566/726 (77.96%), Postives = 644/726 (88.71%), Query Frame = 0

Query: 18  AVNGDWLVGVGSFDMTGPAAGVNMMGYANMDQNTAGIHFRLRARSFVVAKSVDGPRIAFV 77
           A NG++L GVGSFDMTGPAAGVNMMGYAN+ QNTAG+HFRLR R+F+VA+S  GPR AFV
Sbjct: 24  ATNGEYLTGVGSFDMTGPAAGVNMMGYANIFQNTAGVHFRLRTRTFIVAESPQGPRFAFV 83

Query: 78  NLDAGMASQLVTIKVLERLKSRFGNLYTEENVAISGIHTHAGPGGYLQYLVYSITSLGFV 137
           NLDAGMASQLVT KVLERLKSRFGNLYT+ENVAISGIHTHAGPGGYLQYL+YSITS GFV
Sbjct: 84  NLDAGMASQLVTTKVLERLKSRFGNLYTQENVAISGIHTHAGPGGYLQYLLYSITSAGFV 143

Query: 138 QQSFDAIVDAIEQSIVQAHESLKPGFILFNKGDVANAGINRSPSAYLMNPQQERDRYATN 197
           QQSFDAIV AIEQSIVQAH +LKPG +  NKG+V NAGINRSPSAYL NP +ERDRY  N
Sbjct: 144 QQSFDAIVGAIEQSIVQAHNNLKPGSVFINKGEVENAGINRSPSAYLFNPAEERDRYPNN 203

Query: 198 VDKEMTVLKFVDGGSGKNIGAFSWFPTHGTSMSRDNKLISGDNKGAAARFFEDWVASNHT 257
           VD +MT+LK +D GSGK+IGAFSW+ THGTSMSRDNKLISGDNKGAAARFFEDW + N +
Sbjct: 204 VDTQMTLLKLLDRGSGKSIGAFSWYATHGTSMSRDNKLISGDNKGAAARFFEDWFSFNKS 263

Query: 258 TTTFSNHTSDIVELMKKAETIKATGGKRCSKTSSQASKVRKNDGSLFVAAFCQSNVGDVT 317
           ++T  +  ++I  L++KA+TIKATGG+ C KT+SQA KVRKNDGSLFV AFCQSNVGDVT
Sbjct: 264 SSTPISSPAEIETLVEKAQTIKATGGQPCGKTTSQAFKVRKNDGSLFVGAFCQSNVGDVT 323

Query: 318 PNVLGAFCTDSGKPCDFNRSSCHGSDLLCVGRGPGFPDEIRSTKIIGERQFQKAAELFTT 377
           PNVLGAFCTDSGKPCDFNRSSCHG+DLLCVGRGPG+PDEI+STKIIGERQFQKA +LF T
Sbjct: 324 PNVLGAFCTDSGKPCDFNRSSCHGNDLLCVGRGPGYPDEIQSTKIIGERQFQKAVDLFAT 383

Query: 378 ATEKLTGEIDFRHVYLNFTDIQVEIDGNNVVKTCPAALGPGFAAGTTDGPGVFGFQQGDT 437
           ATE+LTG+ID+RHVYLNFT+I VE+DG+ VVKTCPAA+GPGFAAGTTDGPGVFGFQQGDT
Sbjct: 384 ATEELTGKIDYRHVYLNFTNIDVELDGSKVVKTCPAAVGPGFAAGTTDGPGVFGFQQGDT 443

Query: 438 EINKLWKKLRDSLRKPSEFQVDCQKPKPVLLDTGEMFKPYAWAPAILPIQILRLGKLIIL 497
           EI+++WKKLR++L++PS +QV+CQKPK VLLD GEMF PYAWAPAILPIQ+LRLGKLIIL
Sbjct: 444 EISEMWKKLRNALKEPSPYQVECQKPKTVLLDAGEMFDPYAWAPAILPIQMLRLGKLIIL 503

Query: 498 SVPGEFTTMAGRRLREAVKETLISNGNGEFDDDTHVVIAGLTNAYSQYVATFEEYEQQRY 557
           SVPGEFTTMAGRRLREAVKETLISNGNGEFDD T VVIAGLTN YSQYVATFEEYEQQRY
Sbjct: 504 SVPGEFTTMAGRRLREAVKETLISNGNGEFDDKTEVVIAGLTNTYSQYVATFEEYEQQRY 563

Query: 558 EAASTLYGPHTLSAYIQEFKKLATAIAKGEKIVKPIASPPDLSSVQLRFVLDPFGESPPN 617
           EAASTLYGPHTLSAYIQEF KLA A+AKGEK++    +PPDLSSVQL+F+L+P G+SPP 
Sbjct: 564 EAASTLYGPHTLSAYIQEFNKLAKAMAKGEKLISNGPTPPDLSSVQLKFLLEPVGDSPPP 623

Query: 618 GTNFGDIKQDVNLPENGWFKRGGIQKPTATFWSANPRFDLLTEGTYAVVEKLEKQRWTPV 677
           G NFGD+KQD+ +P++G F++G   KP+ATFWSANPR+DLLTEGT+AVVE L+ +RW PV
Sbjct: 624 GKNFGDMKQDIIIPKSGTFRKG--DKPSATFWSANPRYDLLTEGTFAVVEMLQVERWIPV 683

Query: 678 YDDDDISLFFKWTLDNTFMNSLVTIEWDIPINADPGVYRLRHFGSSKSTINSTNTYFTGA 737
           +DDDD  L+FKW++DN+    L TIEW+IP     GVYRLRHFGS+K T  S NTYFTGA
Sbjct: 684 FDDDDFCLYFKWSVDNSSFYGLATIEWEIPEVVSSGVYRLRHFGSAKKTKASPNTYFTGA 743

Query: 738 SQAFAV 744
           S AF V
Sbjct: 744 STAFTV 747

BLAST of ClCG08G009620 vs. ExPASy TrEMBL
Match: A0A2H5PA29 (Neutral ceramidase OS=Citrus unshiu OX=55188 GN=CUMW_117460 PE=3 SV=1)

HSP 1 Score: 1156.7 bits (2991), Expect = 0.0e+00
Identity = 565/736 (76.77%), Postives = 645/736 (87.64%), Query Frame = 0

Query: 15  VIVAVNGDWLVGVGSFDMTGPAAGVNMMGYANMDQNTAGIHFRLRARSFVVAKSVDGPRI 74
           +I + +G +LVGVGS+DMTGPAAG+NMMGYAN+ QNTAGIHFRLRAR+F+VA+S +G R 
Sbjct: 109 LIRSADGQYLVGVGSYDMTGPAAGINMMGYANLFQNTAGIHFRLRARTFIVAESSNGTRF 168

Query: 75  AFVNLDAGMASQLVTIKVLERLKSRFGNLYTEENVAISGIHTHAGPGGYLQYLVYSITSL 134
           AFVNLDAGMASQLVTIKVLE+LK+RFGNLYTEEN+AISGIHTHAGPGGYLQYL+YSITSL
Sbjct: 169 AFVNLDAGMASQLVTIKVLEKLKTRFGNLYTEENIAISGIHTHAGPGGYLQYLIYSITSL 228

Query: 135 GFVQQSFDAIVDAIEQSIVQAHESLKPGFILFNKGDVANAGINRSPSAYLMNPQQERDRY 194
           GFVQQSFDAIV AI QSIVQAH +LKPG I   KGDV NAGINRSPSAYL+NP +ER RY
Sbjct: 229 GFVQQSFDAIVSAIVQSIVQAHNNLKPGSIFIRKGDVENAGINRSPSAYLLNPAEERARY 288

Query: 195 ATNVDKEMTVLKFVDGGSGKNIGAFSWFPTHGTSMSRDNKLISGDNKGAAARFFEDWVAS 254
             NVDKEMT+LKFVD  SGK+IGAF+W+ THGTSMSRDN LISGDNKGAAARFFEDW +S
Sbjct: 289 PENVDKEMTLLKFVDAASGKSIGAFNWYATHGTSMSRDNLLISGDNKGAAARFFEDWFSS 348

Query: 255 NH-----TTTTFSNHTSDIVELMKKAETIKATGGKRCSKTSSQASKVRKNDGSLFVAAFC 314
            +     TT+ FS  +SDI +LM+K++TIKATGG++C KT+SQ  KVRKNDGSLFV AFC
Sbjct: 349 KNESSSITTSKFST-SSDIGKLMEKSKTIKATGGQQCGKTTSQGFKVRKNDGSLFVGAFC 408

Query: 315 QSNVGDVTPNVLGAFCTDSGKPCDFNRSSCHGSDLLCVGRGPGFPDEIRSTKIIGERQFQ 374
           QSNVGDVTPNVLGAFC DSGKPCDFN SSCHG D LC+GRGPG+PDEI STKIIGERQFQ
Sbjct: 409 QSNVGDVTPNVLGAFCIDSGKPCDFNHSSCHGDDQLCIGRGPGYPDEILSTKIIGERQFQ 468

Query: 375 KAAELFTTATEKLTGEIDFRHVYLNFTDIQVEID--GNNVVKTCPAALGPGFAAGTTDGP 434
           KA +LF +AT++L+G+ID+RHVY+NFT+I+V +D  GNN VKTCPAALGPGFAAGTTDGP
Sbjct: 469 KAVDLFNSATDQLSGKIDYRHVYINFTNIEVGLDGTGNNTVKTCPAALGPGFAAGTTDGP 528

Query: 435 GVFGFQQGDTEINKLWKKLRDSLRKPSEFQVDCQKPKPVLLDTGEMFKPYAWAPAILPIQ 494
           GVFGFQQGDTEINKLWK+LRD L+KPS++Q +CQKPK VLL +GEMF+PYAWAPA+LPIQ
Sbjct: 529 GVFGFQQGDTEINKLWKQLRDVLKKPSQYQEECQKPKAVLLSSGEMFEPYAWAPAVLPIQ 588

Query: 495 ILRLGKLIILSVPGEFTTMAGRRLREAVKETLISNGNGEFDDDTHVVIAGLTNAYSQYVA 554
           ILRLGKLIILSVPGEFTTMAGRRLREAVK+TLI+NGNGEFD+DTH+VIAGLTNAYSQY+A
Sbjct: 589 ILRLGKLIILSVPGEFTTMAGRRLREAVKDTLINNGNGEFDNDTHIVIAGLTNAYSQYIA 648

Query: 555 TFEEYEQQRYEAASTLYGPHTLSAYIQEFKKLATAIAKGEKIVKPIASPPDLSSVQLRFV 614
           TFEEY  QRYEAASTLYGPHTL AYIQEFKKLA A+AKGEK  K   SPPDLSSVQL   
Sbjct: 649 TFEEYAHQRYEAASTLYGPHTLPAYIQEFKKLAQAMAKGEKTTKG-PSPPDLSSVQLSLS 708

Query: 615 LDPFGESPPNGTNFGDIKQDVNLPENGWFKRGGIQKPTATFWSANPRFDLLTEGTYAVVE 674
           L+P G+SPP G  FGDIK+D+N P++G F +G   +P+ATFWSANPR+DLLTEGTYAVVE
Sbjct: 709 LNPTGDSPPAGKKFGDIKEDINQPKDGSFNKG--DRPSATFWSANPRYDLLTEGTYAVVE 768

Query: 675 KLEKQRWTPVYDDDDISLFFKWTLDNTFMNSLVTIEWDIPINADPGVYRLRHFGSSKSTI 734
            L+ +RW PVYDDDD SL+FKW+L+NT  + L T+EW++P  A PGVYRLRHFGSSK  +
Sbjct: 769 MLQGKRWIPVYDDDDFSLYFKWSLENTSFHGLATVEWEVPTEAIPGVYRLRHFGSSKKAV 828

Query: 735 NSTNTYFTGASQAFAV 744
           NSTN YFTGAS AF V
Sbjct: 829 NSTNHYFTGASSAFTV 840

BLAST of ClCG08G009620 vs. ExPASy TrEMBL
Match: A0A5E4FCT8 (Neutral ceramidase OS=Prunus dulcis OX=3755 GN=ALMOND_2B003982 PE=3 SV=1)

HSP 1 Score: 1153.7 bits (2983), Expect = 0.0e+00
Identity = 567/732 (77.46%), Postives = 647/732 (88.39%), Query Frame = 0

Query: 15  VIVAVNGD-WLVGVGSFDMTGPAAGVNMMGYANMDQNTAGIHFRLRARSFVVAKSVDGPR 74
           VI   NG+ +L+GVGS+DMTGPAAGVNMMGYANMDQ+TAG+HFRLRAR+F+VA+S  GPR
Sbjct: 15  VIRRCNGEGYLIGVGSYDMTGPAAGVNMMGYANMDQSTAGVHFRLRARTFIVAESSQGPR 74

Query: 75  IAFVNLDAGMASQLVTIKVLERLKSRFGNLYTEENVAISGIHTHAGPGGYLQYLVYSITS 134
            AFVNLDAGMASQLV IKVL++LKSRFG+LYTEENVAISGIHTHAGPGGYLQYLVYSITS
Sbjct: 75  FAFVNLDAGMASQLVNIKVLDKLKSRFGDLYTEENVAISGIHTHAGPGGYLQYLVYSITS 134

Query: 135 LGFVQQSFDAIVDAIEQSIVQAHESLKPGFILFNKGDVANAGINRSPSAYLMNPQQERDR 194
           LGFVQQSFDAIV+AIEQSIVQAH +LKP  +  N+GDV NAGINRSPSAYL+NP +ER R
Sbjct: 135 LGFVQQSFDAIVNAIEQSIVQAHHNLKPASVFINEGDVVNAGINRSPSAYLLNPAEERAR 194

Query: 195 YATNVDKEMTVLKFVDGGSGKNIGAFSWFPTHGTSMSRDNKLISGDNKGAAARFFEDWV- 254
           Y +NVD  MT+LKFVD  SGK++GAFSWF THGTSMS++N LISGDNKGAAARFFEDW  
Sbjct: 195 YPSNVDTLMTLLKFVDDASGKSVGAFSWFATHGTSMSKNNLLISGDNKGAAARFFEDWFS 254

Query: 255 ASNHTTTTFSNHTSDIVELMKKAETIKATGGKRCSKTSSQASKVRKNDGSLFVAAFCQSN 314
           +S+ ++TT ++ ++ ++ L+KKA+++KATGGK C KTSS+ SKVRKN+GSLFV AFCQSN
Sbjct: 255 SSSSSSTTSADSSASLLSLIKKAQSMKATGGKLCGKTSSRESKVRKNNGSLFVGAFCQSN 314

Query: 315 VGDVTPNVLGAFCTDSGKPCDFNRSSCHGSDLLCVGRGPGFPDEIRSTKIIGERQFQKAA 374
           VGDVTPNVLGAFCTDSG+PCDFN SSCHG+DLLC+GRGPG+PDEI STKIIGERQFQKAA
Sbjct: 315 VGDVTPNVLGAFCTDSGRPCDFNHSSCHGNDLLCLGRGPGYPDEILSTKIIGERQFQKAA 374

Query: 375 ELFTTATEKLTGEIDFRHVYLNFTDIQVE-IDGNNVVKTCPAALGPGFAAGTTDGPGVFG 434
            LFTTAT++LTG ID+RHVYLNFTDI+VE +DGN  VKTCPAALGPGFAAGTTDGPG FG
Sbjct: 375 HLFTTATQQLTGTIDYRHVYLNFTDIEVELLDGNKKVKTCPAALGPGFAAGTTDGPGAFG 434

Query: 435 FQQGDTEINKLWKKLRDSLRKPSEFQVDCQKPKPVLLDTGEMFKPYAWAPAILPIQILRL 494
           FQQGDT+INKLW+KLRD+L+KP+ +QVDCQKPK VLLDTGEMF PYAWAPAILPIQ+LRL
Sbjct: 435 FQQGDTKINKLWRKLRDALKKPTPYQVDCQKPKTVLLDTGEMFVPYAWAPAILPIQMLRL 494

Query: 495 GKLIILSVPGEFTTMAGRRLREAVKETLISNGNGEFDDDTHVVIAGLTNAYSQYVATFEE 554
           GKLIILSVPGEFTTMAGRRLREAVKETLISN NGEFDD+TH+VIAGLTN YSQY+ATFEE
Sbjct: 495 GKLIILSVPGEFTTMAGRRLREAVKETLISNSNGEFDDETHIVIAGLTNTYSQYIATFEE 554

Query: 555 YEQQRYEAASTLYGPHTLSAYIQEFKKLATAIAKGEKIVKPIASPPDLSSVQLRFVLDPF 614
           Y QQRYEAASTLYGPHTLSAYIQEF+KLA A+AKGEKI K   SPPDLSSVQLR +L+P 
Sbjct: 555 YAQQRYEAASTLYGPHTLSAYIQEFEKLAKAMAKGEKITKG-PSPPDLSSVQLRLLLEPT 614

Query: 615 GESPPNGTNFGDIKQDVNLPENGWFKRGGIQKPTATFWSANPRFDLLTEGTYAVVEKLEK 674
           G+ PP  TNFGD+KQD+ LP++G FK+G   +P ATFWSANPR+DLLTEGT+AVVE L+ 
Sbjct: 615 GDLPPPHTNFGDMKQDIILPKSGSFKKG--DRPIATFWSANPRYDLLTEGTFAVVELLQG 674

Query: 675 QRWTPVYDDDDISLFFKWTLDNTFMNSLVTIEWDIPINADPGVYRLRHFGSSKSTINSTN 734
           + W PVYDDDD  LFFKW +DN+ +    TIEW+IP +A  GVYRLRHFGSSK T +S N
Sbjct: 675 KHWVPVYDDDDFCLFFKWKVDNSTLYGTATIEWEIPRDAVSGVYRLRHFGSSKKTKDSPN 734

Query: 735 TYFTGASQAFAV 744
            YFTGAS  FAV
Sbjct: 735 IYFTGASSGFAV 743

BLAST of ClCG08G009620 vs. TAIR 10
Match: AT2G38010.1 (Neutral/alkaline non-lysosomal ceramidase )

HSP 1 Score: 854.4 bits (2206), Expect = 7.0e-248
Identity = 443/739 (59.95%), Postives = 542/739 (73.34%), Query Frame = 0

Query: 23  WLVGVGSFDMTGPAAGVNMMGYANMDQNTAGIHFRLRARSFVVAKSVDGPRIAFVNLDAG 82
           +L+GVGS+D+TGPAA VNMMGYAN DQ  +GIHFRLRAR+F+VA+   G R+ FVNLDA 
Sbjct: 26  YLIGVGSYDITGPAADVNMMGYANSDQIASGIHFRLRARAFIVAEP-QGNRVVFVNLDAC 85

Query: 83  MASQLVTIKVLERLKSRFGNLYTEENVAISGIHTHAGPGGYLQYLVYSITSLGFVQQSFD 142
           MASQ+VTIKVLERLK+R+G LYTE+NVAISGIHTHAGPGGYLQY+ Y +TSLGFV+QSFD
Sbjct: 86  MASQIVTIKVLERLKARYGELYTEKNVAISGIHTHAGPGGYLQYVTYIVTSLGFVRQSFD 145

Query: 143 AIVDAIEQSIVQAHESLKPGFILFNKGDVANAGINRSPSAYLMNPQQERDRYATNVDKEM 202
            +V+ IEQSIVQAHESL+PG    NKGD+ +AG+NRSPS+YL NP  ER +Y  +VDKEM
Sbjct: 146 VVVNGIEQSIVQAHESLRPGSAFVNKGDLLDAGVNRSPSSYLNNPAAERSKYKYDVDKEM 205

Query: 203 TVLKFVDGGSGKNIGAFSWFPTHGTSMSRDNKLISGDNKGAAARFFEDW--------VAS 262
           T++KFVD   G   G+F+WF THGTSMSR N LISGDNKGAAARF EDW        V+S
Sbjct: 206 TLVKFVDSQLGPT-GSFNWFATHGTSMSRTNSLISGDNKGAAARFMEDWFENGQKNSVSS 265

Query: 263 NHTTTTFSNHTSDIV----ELMKKAETIKATGGKRCSKTSSQASKVRKNDGSLFVAAFCQ 322
            +     S   SD       L+  A T K++ G    K+    ++VR      FV+AFCQ
Sbjct: 266 RNIPRRVSTIVSDFSRNRDRLLDIAATYKSSRGHSVDKSLDVKTRVRNGSKRKFVSAFCQ 325

Query: 323 SNVGDVTPNVLGAFCTDSGKPCDFNRSSCHGSDLLCVGRGPGFPDEIRSTKIIGERQFQK 382
           SN GDV+PN LG FC D+G PCDFN S+C+G + LC GRGPG+PDE  ST+IIGE+QF+ 
Sbjct: 326 SNCGDVSPNTLGTFCIDTGLPCDFNHSTCNGQNELCYGRGPGYPDEFESTRIIGEKQFKM 385

Query: 383 AAELFTTATEKLTGEIDFRHVYLNFTDIQVEI----DGNNVVKTCPAALGPGFAAGTTDG 442
           A ELF  ATEKL G+I ++H YL+F+++ V +     G+  VKTCPAA+G GFAAGTTDG
Sbjct: 386 AVELFNKATEKLQGKIGYQHAYLDFSNLDVTVPKAGGGSETVKTCPAAMGFGFAAGTTDG 445

Query: 443 PGVFGFQQGDTEINKLWKKLRDSLRKPSEFQVDCQKPKPVLLDTGEMFKPYAWAPAILPI 502
           PG F F+QGD + N  W+ +R+ LR P   QV CQKPKP+LLDTGEM +PY WAP+ILPI
Sbjct: 446 PGAFDFKQGDDQGNVFWRLVRNVLRTPGPEQVQCQKPKPILLDTGEMKEPYDWAPSILPI 505

Query: 503 QILRLGKLIILSVPGEFTTMAGRRLREAVKETLISNGNGEFDDDTHVVIAGLTNAYSQYV 562
           QILR+G+L+ILSVPGEFTTMAGRRLR+A+K  LIS+   EF ++ HVVIAGLTN YSQY+
Sbjct: 506 QILRIGQLVILSVPGEFTTMAGRRLRDAIKSFLISSDPKEFSNNMHVVIAGLTNTYSQYI 565

Query: 563 ATFEEYEQQRYEAASTLYGPHTLSAYIQEFKKLATAIAKGEKIVKPIASPPDLSSVQLRF 622
           ATFEEYE QRYE ASTLYG HTL+AYIQEFKKLATA+  G  + +    PPDL   Q+  
Sbjct: 566 ATFEEYEVQRYEGASTLYGRHTLTAYIQEFKKLATALVNGLTLPRG-PQPPDLLDKQISL 625

Query: 623 VLDPFGESPPNGTNFGDIKQDVNLPENGWFKRGGIQKPTATFWSANPRFDLLTEGTYAVV 682
           +     +S P G  FGD+K DV  P    F+RG  Q+  ATFWS  PR DL+TEG++AVV
Sbjct: 626 LSPVVVDSTPLGVKFGDVKADV--PPKSTFRRG--QQVNATFWSGCPRNDLMTEGSFAVV 685

Query: 683 EKL-EKQRWTPVYDDDDISLFFKWTLDNTFMN-SLVTIEWDIPINADPGVYRLRHFGSSK 742
           E L E  +W PVYDDDD SL FKW+      + S  TIEW +P +A  GVYR+RH+G+SK
Sbjct: 686 ETLREGGKWAPVYDDDDFSLKFKWSRPAKLSSESQATIEWRVPESAVAGVYRIRHYGASK 745

Query: 743 STINSTNTYFTGASQAFAV 744
           S   S ++ F+G+S AF V
Sbjct: 746 SLFGSISS-FSGSSSAFVV 756

BLAST of ClCG08G009620 vs. TAIR 10
Match: AT1G07380.1 (Neutral/alkaline non-lysosomal ceramidase )

HSP 1 Score: 841.3 bits (2172), Expect = 6.1e-244
Identity = 432/752 (57.45%), Postives = 549/752 (73.01%), Query Frame = 0

Query: 20  NGDWLVGVGSFDMTGPAAGVNMMGYANMDQNTAGIHFRLRARSFVVAKSVDGPRIAFVNL 79
           + ++L+G+GS+D+TGPAA VNMMGYANM+Q  +GIHFRLRAR+F+V++   G R+ FVNL
Sbjct: 34  HSEYLIGLGSYDITGPAADVNMMGYANMEQVASGIHFRLRARTFIVSEP-QGKRVVFVNL 93

Query: 80  DAGMASQLVTIKVLERLKSRFGNLYTEENVAISGIHTHAGPGGYLQYLVYSITSLGFVQQ 139
           DA MASQ+V +KV+ERLK+R+G+LYTE+NV ISGIHTHAGPGGYLQY+VY +TSLGFV+Q
Sbjct: 94  DACMASQIVKLKVIERLKARYGDLYTEQNVGISGIHTHAGPGGYLQYVVYIVTSLGFVRQ 153

Query: 140 SFDAIVDAIEQSIVQAHESLKPGFILFNKGDVANAGINRSPSAYLMNPQQERDRYATNVD 199
           SFDA+VD IE SI+QAHE+L+PG I  N G++ +AG+NRSPSAYL NP +ER ++  NVD
Sbjct: 154 SFDALVDGIENSIIQAHENLRPGSIFLNNGELLDAGVNRSPSAYLNNPSKERSKHKYNVD 213

Query: 200 KEMTVLKFVDGGSGKNIGAFSWFPTHGTSMSRDNKLISGDNKGAAARFFEDWVASNHTTT 259
           KEMT+LKFVD   G  +G+F+WF THGTSMSR N LISGDNKGAA+RF EDW   N    
Sbjct: 214 KEMTLLKFVDDQWGP-VGSFNWFATHGTSMSRTNSLISGDNKGAASRFMEDWYEQNTAER 273

Query: 260 TFS-----------------NHTSDIVELMKKAETIKATGGKRCSKTSSQASKV----RK 319
           ++S                 NH     EL++ A   ++  GK  ++ SS A +V    RK
Sbjct: 274 SYSEEFISDEIPRRVSSLIENHQDSHHELLELASYFESQPGKPVTRISSSARRVRSALRK 333

Query: 320 NDGSLFVAAFCQSNVGDVTPNVLGAFCTDSGKPCDFNRSSCHGSDLLCVGRGPGFPDEIR 379
            D   FV+AFCQ+N GDV+PNVLGAFC D+G PCDFN S+C G + +C GRGPG+PDE  
Sbjct: 334 ADKPGFVSAFCQTNCGDVSPNVLGAFCLDTGLPCDFNHSTCGGKNEMCYGRGPGYPDEFE 393

Query: 380 STKIIGERQFQKAAELFTTATEKLTGEIDFRHVYLNFTDIQVEI---DG-NNVVKTCPAA 439
           ST+IIGERQF+ A ELF  A+E+L G++D+RHVY++F+ + V +   DG + VVKTCPAA
Sbjct: 394 STRIIGERQFKMALELFNKASEQLQGKVDYRHVYVDFSQLNVTLPKKDGKSEVVKTCPAA 453

Query: 440 LGPGFAAGTTDGPGVFGFQQGDTEINKLWKKLRDSLRKPSEFQVDCQKPKPVLLDTGEMF 499
           +G  FAAGTTDGPG F F QGD + N  W+ +R+ L+ P + Q+DC  PKP+LLDTGEM 
Sbjct: 454 MGFAFAAGTTDGPGAFDFTQGDDKGNPFWRLVRNVLKTPDKKQIDCHYPKPILLDTGEMT 513

Query: 500 KPYAWAPAILPIQILRLGKLIILSVPGEFTTMAGRRLREAVKETLISNGNGEFDDDTHVV 559
           KPY WAP+IL +Q+LR+G+L ILSVPGEFTTMAGRRLR AVK  L ++GN +   + HVV
Sbjct: 514 KPYDWAPSILSLQVLRIGQLFILSVPGEFTTMAGRRLRYAVKTQLKNSGNKDLSGEIHVV 573

Query: 560 IAGLTNAYSQYVATFEEYEQQRYEAASTLYGPHTLSAYIQEFKKLATAIAKGEKIVKPIA 619
           IAGL N YSQYV TFEEY+ QRYE ASTLYGPHTLS YIQEFKKL+ ++   +  V+P  
Sbjct: 574 IAGLANGYSQYVTTFEEYQVQRYEGASTLYGPHTLSGYIQEFKKLSKSLVL-DMPVQPGP 633

Query: 620 SPPDLSSVQLRFVLDPFGESPPNGTNFGDIKQDVNLPENGWFKRGGIQKPTATFWSANPR 679
            PPDL   QL F+     ++ P+G +FGD+  DV  P+N   KRG  Q  T  F SA PR
Sbjct: 634 QPPDLLDKQLSFLTPVMMDTTPSGDSFGDVISDV--PKNLSLKRGNGQ-VTVVFRSACPR 693

Query: 680 FDLLTEGTYAVVEKLEK--QRWTPVYDDDDISLFFKWTLDNTFMN-SLVTIEWDIPINAD 739
            DLLTEGT+ +VE+LE+  + WTPVYDDDD+ L FKW+      + S  T+EW IP +A 
Sbjct: 694 NDLLTEGTFTLVERLEQKDKTWTPVYDDDDLCLRFKWSRHKKLSSRSQATVEWRIPESAS 753

Query: 740 PGVYRLRHFGSSKSTINSTNTYFTGASQAFAV 744
           PGVYR+ HFG++K    S + +FTG+S AF V
Sbjct: 754 PGVYRITHFGAAKKLFGSVH-HFTGSSSAFVV 778

BLAST of ClCG08G009620 vs. TAIR 10
Match: AT2G38010.2 (Neutral/alkaline non-lysosomal ceramidase )

HSP 1 Score: 840.9 bits (2171), Expect = 8.0e-244
Identity = 443/774 (57.24%), Postives = 542/774 (70.03%), Query Frame = 0

Query: 23  WLVGVGSFDMTGPAAGVNMMGYANMDQNTAGIHFRLRARSFVVAKSVDGPRIAFVNLDAG 82
           +L+GVGS+D+TGPAA VNMMGYAN DQ  +GIHFRLRAR+F+VA+   G R+ FVNLDA 
Sbjct: 26  YLIGVGSYDITGPAADVNMMGYANSDQIASGIHFRLRARAFIVAEP-QGNRVVFVNLDAC 85

Query: 83  MASQLVTIKVLERLKSRFGNLYTEENVAISGIHTHAGPGGYLQYLVYSITSLGFVQQSFD 142
           MASQ+VTIKVLERLK+R+G LYTE+NVAISGIHTHAGPGGYLQY+ Y +TSLGFV+QSFD
Sbjct: 86  MASQIVTIKVLERLKARYGELYTEKNVAISGIHTHAGPGGYLQYVTYIVTSLGFVRQSFD 145

Query: 143 AIVDAIEQSIVQAHESLKPGFILFNKGDVANAGINRSPSAYLMNPQQERDRYATNVDKEM 202
            +V+ IEQSIVQAHESL+PG    NKGD+ +AG+NRSPS+YL NP  ER +Y  +VDKEM
Sbjct: 146 VVVNGIEQSIVQAHESLRPGSAFVNKGDLLDAGVNRSPSSYLNNPAAERSKYKYDVDKEM 205

Query: 203 TVLKFVDGGSGKNIGAFSWFPTHGTSMSRDNKLISGDNKGAAARFFEDW--------VAS 262
           T++KFVD   G   G+F+WF THGTSMSR N LISGDNKGAAARF EDW        V+S
Sbjct: 206 TLVKFVDSQLGPT-GSFNWFATHGTSMSRTNSLISGDNKGAAARFMEDWFENGQKNSVSS 265

Query: 263 NHTTTTFSNHTSDI---------------------------------------VELMKKA 322
            +     S   SD                                          L+  A
Sbjct: 266 RNIPRRVSTIVSDFSRNRESYPFLELPNVAILYIAVAERLMWKVMVLFYFVSESRLLDIA 325

Query: 323 ETIKATGGKRCSKTSSQASKVRKNDGSLFVAAFCQSNVGDVTPNVLGAFCTDSGKPCDFN 382
            T K++ G    K+    ++VR      FV+AFCQSN GDV+PN LG FC D+G PCDFN
Sbjct: 326 ATYKSSRGHSVDKSLDVKTRVRNGSKRKFVSAFCQSNCGDVSPNTLGTFCIDTGLPCDFN 385

Query: 383 RSSCHGSDLLCVGRGPGFPDEIRSTKIIGERQFQKAAELFTTATEKLTGEIDFRHVYLNF 442
            S+C+G + LC GRGPG+PDE  ST+IIGE+QF+ A ELF  ATEKL G+I ++H YL+F
Sbjct: 386 HSTCNGQNELCYGRGPGYPDEFESTRIIGEKQFKMAVELFNKATEKLQGKIGYQHAYLDF 445

Query: 443 TDIQVEI----DGNNVVKTCPAALGPGFAAGTTDGPGVFGFQQGDTEINKLWKKLRDSLR 502
           +++ V +     G+  VKTCPAA+G GFAAGTTDGPG F F+QGD + N  W+ +R+ LR
Sbjct: 446 SNLDVTVPKAGGGSETVKTCPAAMGFGFAAGTTDGPGAFDFKQGDDQGNVFWRLVRNVLR 505

Query: 503 KPSEFQVDCQKPKPVLLDTGEMFKPYAWAPAILPIQILRLGKLIILSVPGEFTTMAGRRL 562
            P   QV CQKPKP+LLDTGEM +PY WAP+ILPIQILR+G+L+ILSVPGEFTTMAGRRL
Sbjct: 506 TPGPEQVQCQKPKPILLDTGEMKEPYDWAPSILPIQILRIGQLVILSVPGEFTTMAGRRL 565

Query: 563 REAVKETLISNGNGEFDDDTHVVIAGLTNAYSQYVATFEEYEQQRYEAASTLYGPHTLSA 622
           R+A+K  LIS+   EF ++ HVVIAGLTN YSQY+ATFEEYE QRYE ASTLYG HTL+A
Sbjct: 566 RDAIKSFLISSDPKEFSNNMHVVIAGLTNTYSQYIATFEEYEVQRYEGASTLYGRHTLTA 625

Query: 623 YIQEFKKLATAIAKGEKIVKPIASPPDLSSVQLRFVLDPFGESPPNGTNFGDIKQDVNLP 682
           YIQEFKKLATA+  G  + +    PPDL   Q+  +     +S P G  FGD+K DV  P
Sbjct: 626 YIQEFKKLATALVNGLTLPRG-PQPPDLLDKQISLLSPVVVDSTPLGVKFGDVKADV--P 685

Query: 683 ENGWFKRGGIQKPTATFWSANPRFDLLTEGTYAVVEKL-EKQRWTPVYDDDDISLFFKWT 742
               F+RG  Q+  ATFWS  PR DL+TEG++AVVE L E  +W PVYDDDD SL FKW+
Sbjct: 686 PKSTFRRG--QQVNATFWSGCPRNDLMTEGSFAVVETLREGGKWAPVYDDDDFSLKFKWS 745

Query: 743 LDNTFMN-SLVTIEWDIPINADPGVYRLRHFGSSKSTINSTNTYFTGASQAFAV 744
                 + S  TIEW +P +A  GVYR+RH+G+SKS   S ++ F+G+S AF V
Sbjct: 746 RPAKLSSESQATIEWRVPESAVAGVYRIRHYGASKSLFGSISS-FSGSSSAFVV 791

BLAST of ClCG08G009620 vs. TAIR 10
Match: AT5G58980.1 (Neutral/alkaline non-lysosomal ceramidase )

HSP 1 Score: 790.0 bits (2039), Expect = 1.6e-228
Identity = 420/769 (54.62%), Postives = 533/769 (69.31%), Query Frame = 0

Query: 2   RWQL-----LKRLMAMATVIVAVNGDWLVGVGSFDMTGPAAGVNMMGYANMDQNTAGIHF 61
           RW +     L  L  +    +  + D+L+G+GS+D+TGPAA VNMMGYANM+Q  +G+HF
Sbjct: 3   RWSMSMHCTLFLLFLLRLTCIFSDSDYLMGLGSYDITGPAADVNMMGYANMEQVASGVHF 62

Query: 62  RLRARSFVVAKSVDGPRIAFVNLDAGMASQLVTIKVLERLKSRFGNLYTEENVAISGIHT 121
           RLRAR+F+VA+     RIAFVNLDAGMASQLVTIKV+ERLK R+G LYTEENVAISG HT
Sbjct: 63  RLRARAFIVAEPYK-KRIAFVNLDAGMASQLVTIKVIERLKQRYGELYTEENVAISGTHT 122

Query: 122 HAGPGGYLQYLVYSITSLGFVQQSFDAIVDAIEQSIVQAHESLKPGFILFNKGDVANAGI 181
           HAGPGGYLQY++Y +TSLGFV QSF+A+VD IEQSI+QAHE+L+PG IL NKG++ +AG+
Sbjct: 123 HAGPGGYLQYILYLVTSLGFVHQSFNALVDGIEQSIIQAHENLRPGSILINKGELLDAGV 182

Query: 182 NRSPSAYLMNPQQERDRYATNVDKEMTVLKFVDGGSGKNIGAFSWFPTHGTSMSRDNKLI 241
           NRSPSAYL NP  ER +Y  +VDKEMT++KFVD                           
Sbjct: 183 NRSPSAYLNNPAHERSKYEYDVDKEMTLVKFVD--------------------------- 242

Query: 242 SGDNKGAAARFFEDWVASNHTTTTFS----NHTSDIV------ELMKKAETIKATGGKRC 301
             D  G  AR  EDW    +   +         S I+      +LM+ A ++ +TGGK  
Sbjct: 243 --DQWGPVARIMEDWFERENGCRSVDVESPRRVSSIISDPYDQDLMEMASSLLSTGGKTV 302

Query: 302 SKTSSQASKVRKN----DGSLFVAAFCQSNVGDVTPNVLGAFCTDSGKPCDFNRSSCHGS 361
           ++ SS A +VR      D   FV+AFCQ+N GDV+PNVLGAFC D+G PC+FN+S+C G 
Sbjct: 303 TRMSSVARRVRSRFRHADKPRFVSAFCQTNCGDVSPNVLGAFCIDTGLPCEFNQSTCGGK 362

Query: 362 DLLCVGRGPGFPDEIRSTKIIGERQFQKAAELFTTATEKLTGEIDFRHVYLNFTDIQVEI 421
           +  C GRGPG+PDE  ST+IIGERQF+KAA+LFT A+E++ G++D+RH Y++F+ ++V I
Sbjct: 363 NEQCYGRGPGYPDEFESTRIIGERQFKKAADLFTKASEEIQGKVDYRHAYVDFSQLEVTI 422

Query: 422 DGNN----VVKTCPAALGPGFAAGTTDGPGVFGFQQGDTEINKLWKKLRDSLRKPSEFQV 481
           +G N    VVKTCPAA+G GFAAGTTDGPG F F+QGD + N  W+ +R+ L+ P+E QV
Sbjct: 423 NGQNGGSEVVKTCPAAMGFGFAAGTTDGPGAFDFKQGDDQGNPFWRLVRNLLKNPTEEQV 482

Query: 482 DCQKPKPVLLDTGEMFKPYAWAPAILPIQILRLGKLIILSVPGEFTTMAGRRLREAVKET 541
            CQ+PKP+LLDTGEM +PY WAP+ILP+QILR+G+L+IL VPGEFTTMAGRRLR+AVK  
Sbjct: 483 RCQRPKPILLDTGEMKQPYDWAPSILPVQILRIGQLVILCVPGEFTTMAGRRLRDAVKTV 542

Query: 542 LISNGNGEFDDDTHVVIAGLTNAYSQYVATFEEYEQQRYEAASTLYGPHTLSAYIQEFKK 601
           L    NG    +  VVIAGLTN+YSQY+ATFEEY+ QRYE ASTLYGPHTLS YIQEFKK
Sbjct: 543 LKEGSNGR---EFSVVIAGLTNSYSQYIATFEEYQVQRYEGASTLYGPHTLSGYIQEFKK 602

Query: 602 LATAIAKGEKIVKPIASPPDLSSVQLRFVLDPFGESPPNGTNFGDIKQDVNLPENGWFKR 661
           LA  +   +    P   PPDL   Q+  +     +  P GT FGD+  DV  P    F++
Sbjct: 603 LANDLLSAQ-TTDPGPQPPDLLHKQISLLTPVVADMTPIGTAFGDVTSDV--PRLSKFRK 662

Query: 662 GGIQKPTATFWSANPRFDLLTEGTYAVVEK-LE-KQRWTPVYDDDDISLFFKWTLD-NTF 721
           G        F SANPR DL+TEGT+A+VE+ LE ++ W PVYDDDD  L FKW+      
Sbjct: 663 GA-DIVRVQFRSANPRNDLMTEGTFALVERWLEGRETWVPVYDDDDFCLRFKWSRPFKLS 722

Query: 722 MNSLVTIEWDIPINADPGVYRLRHFGSSKSTINSTNTYFTGASQAFAVF 745
             S  TIEW IP  A PGVYR+ HFGS+K+ I+S + +F+G+S AF V+
Sbjct: 723 TQSTATIEWRIPETASPGVYRITHFGSAKTPISSIH-HFSGSSSAFVVY 733