ClCG08G009152 (gene) Watermelon (Charleston Gray) v2.5

Overview
NameClCG08G009152
Typegene
OrganismCitrullus lanatus subsp. vulgaris cv. Charleston Gray (Watermelon (Charleston Gray) v2.5)
DescriptionBeta-galactosidase
LocationCG_Chr08: 21770914 .. 21771195 (+)
RNA-Seq ExpressionClCG08G009152
SyntenyClCG08G009152
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: exonCDSpolypeptide
Hold the cursor over a type above to highlight its positions in the sequence below.
ATGTTTAAGCTTCAACTATTTGTTCTTCTTCTTATTGCTACTTTAGCTTGCTTATCTTTCTATACAGGAGTAAATGTCACCTATGACTCAAATGCTATAATTATCAATGGTGAACGACGCATTATCTTCTCAGGTTCAATCCATTATCCACGTAGTACTGAAAAGATGTGGCCTGATCTTATTAAGAAAGCTTGGATGGTGGACTCGATGCAATTGAGACATACATTTTTTGGGATCGTCATGAGCTGCAACGACGAAAATATGATTTCTCTGGAAATCTAG

mRNA sequence

ATGTTTAAGCTTCAACTATTTGTTCTTCTTCTTATTGCTACTTTAGCTTGCTTATCTTTCTATACAGGAGTAAATGTCACCTATGACTCAAATGCTATAATTATCAATGGTGAACGACGCATTATCTTCTCAGGTTCAATCCATTATCCACGTAGTACTGAAAAGATGTGGCCTGATCTTATTAAGAAAGCTTGGATGGTGGACTCGATGCAATTGAGACATACATTTTTTGGGATCGTCATGAGCTGCAACGACGAAAATATGATTTCTCTGGAAATCTAG

Coding sequence (CDS)

ATGTTTAAGCTTCAACTATTTGTTCTTCTTCTTATTGCTACTTTAGCTTGCTTATCTTTCTATACAGGAGTAAATGTCACCTATGACTCAAATGCTATAATTATCAATGGTGAACGACGCATTATCTTCTCAGGTTCAATCCATTATCCACGTAGTACTGAAAAGATGTGGCCTGATCTTATTAAGAAAGCTTGGATGGTGGACTCGATGCAATTGAGACATACATTTTTTGGGATCGTCATGAGCTGCAACGACGAAAATATGATTTCTCTGGAAATCTAG

Protein sequence

MFKLQLFVLLLIATLACLSFYTGVNVTYDSNAIIINGERRIIFSGSIHYPRSTEKMWPDLIKKAWMVDSMQLRHTFFGIVMSCNDENMISLEI
Homology
[Close]
BLAST of ClCG08G009152 vs. NCBI nr
Match: KAA0045403.1 (beta-galactosidase-like [Cucumis melo var. makuwa] >TYK11334.1 beta-galactosidase-like [Cucumis melo var. makuwa])

HSP 1 Score: 108.2 bits (269), Expect = 3.8e-20
Identity = 55/64 (85.94%), Postives = 56/64 (87.50%), Query Frame = 0 

Query: 1  MFKLQLFVLLLIATLACLSFYTGVNVTYDSNAIIINGERRIIFSGSIHYPRSTEKMWPDL 60
          MFKL   VLLLIA LACLSF TG NVTYDSNAIIINGERRII SGSIHYPRSTE MWPDL
Sbjct: 1  MFKLHWLVLLLIAALACLSFCTGANVTYDSNAIIINGERRIILSGSIHYPRSTENMWPDL 60

Query: 61 IKKA 65
          I+KA
Sbjct: 61 IQKA 64

[Close]
BLAST of ClCG08G009152 vs. NCBI nr
Match: XP_008464891.1 (PREDICTED: beta-galactosidase-like [Cucumis melo])

HSP 1 Score: 108.2 bits (269), Expect = 3.8e-20
Identity = 55/64 (85.94%), Postives = 56/64 (87.50%), Query Frame = 0 

Query: 1  MFKLQLFVLLLIATLACLSFYTGVNVTYDSNAIIINGERRIIFSGSIHYPRSTEKMWPDL 60
          MFKL   VLLLIA LACLSF TG NVTYDSNAIIINGERRII SGSIHYPRSTE MWPDL
Sbjct: 1  MFKLHWLVLLLIAALACLSFCTGANVTYDSNAIIINGERRIILSGSIHYPRSTENMWPDL 60

Query: 61 IKKA 65
          I+KA
Sbjct: 61 IQKA 64

[Close]
BLAST of ClCG08G009152 vs. NCBI nr
Match: XP_038893262.1 (beta-galactosidase 7-like [Benincasa hispida])

HSP 1 Score: 104.0 bits (258), Expect = 7.2e-19
Identity = 52/64 (81.25%), Postives = 56/64 (87.50%), Query Frame = 0 

Query: 1  MFKLQLFVLLLIATLACLSFYTGVNVTYDSNAIIINGERRIIFSGSIHYPRSTEKMWPDL 60
          MFKLQ  V+ LIATLAC+SF  G NV+YDSNAIIINGER IIFSGSIHYPRST +MWPDL
Sbjct: 1  MFKLQYLVVRLIATLACISFCIGANVSYDSNAIIINGERHIIFSGSIHYPRSTVQMWPDL 60

Query: 61 IKKA 65
          IKKA
Sbjct: 61 IKKA 64

[Close]
BLAST of ClCG08G009152 vs. NCBI nr
Match: XP_008459141.1 (PREDICTED: beta-galactosidase 15-like [Cucumis melo])

HSP 1 Score: 102.4 bits (254), Expect = 2.1e-18
Identity = 53/64 (82.81%), Postives = 54/64 (84.38%), Query Frame = 0 

Query: 1  MFKLQLFVLLLIATLACLSFYTGVNVTYDSNAIIINGERRIIFSGSIHYPRSTEKMWPDL 60
          MF L   VLLLIA LAC SF TG NVTYDSNAIIINGERRII SGSIHYPRSTE MWPDL
Sbjct: 1  MFNLHWSVLLLIAALACSSFCTGENVTYDSNAIIINGERRIILSGSIHYPRSTENMWPDL 60

Query: 61 IKKA 65
          I+KA
Sbjct: 61 IQKA 64

[Close]
BLAST of ClCG08G009152 vs. NCBI nr
Match: XP_022961111.1 (beta-galactosidase 7-like [Cucurbita moschata])

HSP 1 Score: 100.1 bits (248), Expect = 1.0e-17
Identity = 49/64 (76.56%), Postives = 56/64 (87.50%), Query Frame = 0 

Query: 1  MFKLQLFVLLLIATLACLSFYTGVNVTYDSNAIIINGERRIIFSGSIHYPRSTEKMWPDL 60
          MF ++  VL L+A+LACLSF T  NVTYDSNA+IINGERRIIFSGSIHYPRST+ MWPDL
Sbjct: 1  MFNIRSLVLSLVASLACLSFSTADNVTYDSNALIINGERRIIFSGSIHYPRSTDAMWPDL 60

Query: 61 IKKA 65
          I+KA
Sbjct: 61 IQKA 64

[Close]
BLAST of ClCG08G009152 vs. ExPASy Swiss-Prot
Match: Q9LFA6 (Beta-galactosidase 2 OS=Arabidopsis thaliana OX=3702 GN=BGAL2 PE=2 SV=2)

HSP 1 Score: 73.6 bits (179), Expect = 1.4e-12
Identity = 36/63 (57.14%), Postives = 46/63 (73.02%), Query Frame = 0 

Query: 2  FKLQLFVLLLIATLACLSFYTGVNVTYDSNAIIINGERRIIFSGSIHYPRSTEKMWPDLI 61
          F+ + +++L I   + L   T   VTYD  A+IING+RRI+ SGSIHYPRST +MWPDLI
Sbjct: 5  FRNKAWIILAILCFSSLIHSTEAVVTYDHKALIINGQRRILISGSIHYPRSTPEMWPDLI 64

Query: 62 KKA 65
          KKA
Sbjct: 65 KKA 67

[Close]
BLAST of ClCG08G009152 vs. ExPASy Swiss-Prot
Match: Q67VU7 (Putative beta-galactosidase 10 OS=Oryza sativa subsp. japonica OX=39947 GN=Os06g0628500 PE=3 SV=1)

HSP 1 Score: 72.8 bits (177), Expect = 2.3e-12
Identity = 35/57 (61.40%), Postives = 43/57 (75.44%), Query Frame = 0 

Query: 8  VLLLIATLACLSFYTGVNVTYDSNAIIINGERRIIFSGSIHYPRSTEKMWPDLIKKA 65
          V LL+  LA  +   G  VTY+  +++I+GERRII SGSIHYPRST +MWPDLIKKA
Sbjct: 13 VALLVVVLAEAAGVGGTTVTYNDRSLVIDGERRIIISGSIHYPRSTPEMWPDLIKKA 69

[Close]
BLAST of ClCG08G009152 vs. ExPASy Swiss-Prot
Match: Q9SCV0 (Beta-galactosidase 12 OS=Arabidopsis thaliana OX=3702 GN=BGAL12 PE=2 SV=1)

HSP 1 Score: 72.8 bits (177), Expect = 2.3e-12
Identity = 35/57 (61.40%), Postives = 42/57 (73.68%), Query Frame = 0 

Query: 10 LLIATLACLSFYTGVN--VTYDSNAIIINGERRIIFSGSIHYPRSTEKMWPDLIKKA 65
          +L+  L C S    V   VTYD  A+IING+RRI+ SGSIHYPRST +MWPDLI+KA
Sbjct: 11 ILLGILCCSSLICSVKAIVTYDRKAVIINGQRRILLSGSIHYPRSTPEMWPDLIQKA 67

[Close]
BLAST of ClCG08G009152 vs. ExPASy Swiss-Prot
Match: Q8W0A1 (Beta-galactosidase 2 OS=Oryza sativa subsp. japonica OX=39947 GN=Os01g0580200 PE=2 SV=1)

HSP 1 Score: 71.6 bits (174), Expect = 5.2e-12
Identity = 32/57 (56.14%), Postives = 42/57 (73.68%), Query Frame = 0 

Query: 8  VLLLIATLACLSFYTGVNVTYDSNAIIINGERRIIFSGSIHYPRSTEKMWPDLIKKA 65
          V  ++A  A L+      VTYD  A+++NG+RRI+ SGSIHYPRST +MWPDLI+KA
Sbjct: 8  VAFVVAVAAVLAAAASAAVTYDRKAVVVNGQRRILISGSIHYPRSTPEMWPDLIEKA 64

[Close]
BLAST of ClCG08G009152 vs. ExPASy Swiss-Prot
Match: P45582 (Beta-galactosidase OS=Asparagus officinalis OX=4686 PE=2 SV=1)

HSP 1 Score: 71.2 bits (173), Expect = 6.8e-12
Identity = 36/63 (57.14%), Postives = 49/63 (77.78%), Query Frame = 0 

Query: 4  LQLFVLLLIATLACLSFYTGV--NVTYDSNAIIINGERRIIFSGSIHYPRSTEKMWPDLI 63
          L+L ++L++A LA +     V  +VTYD  ++IING+RRI+ SGSIHYPRST +MWPDLI
Sbjct: 3  LKLVLMLMVALLAAVWSPPAVTASVTYDHKSVIINGQRRILISGSIHYPRSTPEMWPDLI 62

Query: 64 KKA 65
          +KA
Sbjct: 63 QKA 65

[Close]
BLAST of ClCG08G009152 vs. ExPASy TrEMBL
Match: A0A5A7TPD9 (Beta-galactosidase OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold807G00080 PE=3 SV=1)

HSP 1 Score: 108.2 bits (269), Expect = 1.8e-20
Identity = 55/64 (85.94%), Postives = 56/64 (87.50%), Query Frame = 0 

Query: 1  MFKLQLFVLLLIATLACLSFYTGVNVTYDSNAIIINGERRIIFSGSIHYPRSTEKMWPDL 60
          MFKL   VLLLIA LACLSF TG NVTYDSNAIIINGERRII SGSIHYPRSTE MWPDL
Sbjct: 1  MFKLHWLVLLLIAALACLSFCTGANVTYDSNAIIINGERRIILSGSIHYPRSTENMWPDL 60

Query: 61 IKKA 65
          I+KA
Sbjct: 61 IQKA 64

[Close]
BLAST of ClCG08G009152 vs. ExPASy TrEMBL
Match: A0A1S3CP45 (Beta-galactosidase OS=Cucumis melo OX=3656 GN=LOC103502650 PE=3 SV=1)

HSP 1 Score: 108.2 bits (269), Expect = 1.8e-20
Identity = 55/64 (85.94%), Postives = 56/64 (87.50%), Query Frame = 0 

Query: 1  MFKLQLFVLLLIATLACLSFYTGVNVTYDSNAIIINGERRIIFSGSIHYPRSTEKMWPDL 60
          MFKL   VLLLIA LACLSF TG NVTYDSNAIIINGERRII SGSIHYPRSTE MWPDL
Sbjct: 1  MFKLHWLVLLLIAALACLSFCTGANVTYDSNAIIINGERRIILSGSIHYPRSTENMWPDL 60

Query: 61 IKKA 65
          I+KA
Sbjct: 61 IQKA 64

[Close]
BLAST of ClCG08G009152 vs. ExPASy TrEMBL
Match: A0A1S3C8Z9 (Beta-galactosidase OS=Cucumis melo OX=3656 GN=LOC103498345 PE=3 SV=1)

HSP 1 Score: 102.4 bits (254), Expect = 1.0e-18
Identity = 53/64 (82.81%), Postives = 54/64 (84.38%), Query Frame = 0 

Query: 1  MFKLQLFVLLLIATLACLSFYTGVNVTYDSNAIIINGERRIIFSGSIHYPRSTEKMWPDL 60
          MF L   VLLLIA LAC SF TG NVTYDSNAIIINGERRII SGSIHYPRSTE MWPDL
Sbjct: 1  MFNLHWSVLLLIAALACSSFCTGENVTYDSNAIIINGERRIILSGSIHYPRSTENMWPDL 60

Query: 61 IKKA 65
          I+KA
Sbjct: 61 IQKA 64

[Close]
BLAST of ClCG08G009152 vs. ExPASy TrEMBL
Match: A0A6J1H9F6 (Beta-galactosidase OS=Cucurbita moschata OX=3662 GN=LOC111461719 PE=3 SV=1)

HSP 1 Score: 100.1 bits (248), Expect = 5.0e-18
Identity = 49/64 (76.56%), Postives = 56/64 (87.50%), Query Frame = 0 

Query: 1  MFKLQLFVLLLIATLACLSFYTGVNVTYDSNAIIINGERRIIFSGSIHYPRSTEKMWPDL 60
          MF ++  VL L+A+LACLSF T  NVTYDSNA+IINGERRIIFSGSIHYPRST+ MWPDL
Sbjct: 1  MFNIRSLVLSLVASLACLSFSTADNVTYDSNALIINGERRIIFSGSIHYPRSTDAMWPDL 60

Query: 61 IKKA 65
          I+KA
Sbjct: 61 IQKA 64

[Close]
BLAST of ClCG08G009152 vs. ExPASy TrEMBL
Match: A0A0A0LDH9 (Beta-galactosidase OS=Cucumis sativus OX=3659 GN=Csa_3G550690 PE=3 SV=1)

HSP 1 Score: 99.4 bits (246), Expect = 8.6e-18
Identity = 53/65 (81.54%), Postives = 58/65 (89.23%), Query Frame = 0 

Query: 1  MFKLQLFVLL-LIATLACLSFYTGVNVTYDSNAIIINGERRIIFSGSIHYPRSTEKMWPD 60
          MFKL  ++LL L ATLACLSF  G NV+YDSNAIIINGERRIIFSGSIHYPRSTE+MWPD
Sbjct: 1  MFKLFQWLLLDLAATLACLSFCIGNNVSYDSNAIIINGERRIIFSGSIHYPRSTEEMWPD 60

Query: 61 LIKKA 65
          LI+KA
Sbjct: 61 LIQKA 65

[Close]
BLAST of ClCG08G009152 vs. TAIR 10
Match: AT3G52840.1 (beta-galactosidase 2 )

HSP 1 Score: 73.6 bits (179), Expect = 9.7e-14
Identity = 36/63 (57.14%), Postives = 46/63 (73.02%), Query Frame = 0 

Query: 2  FKLQLFVLLLIATLACLSFYTGVNVTYDSNAIIINGERRIIFSGSIHYPRSTEKMWPDLI 61
          F+ + +++L I   + L   T   VTYD  A+IING+RRI+ SGSIHYPRST +MWPDLI
Sbjct: 5  FRNKAWIILAILCFSSLIHSTEAVVTYDHKALIINGQRRILISGSIHYPRSTPEMWPDLI 64

Query: 62 KKA 65
          KKA
Sbjct: 65 KKA 67

[Close]
BLAST of ClCG08G009152 vs. TAIR 10
Match: AT4G26140.1 (beta-galactosidase 12 )

HSP 1 Score: 72.8 bits (177), Expect = 1.7e-13
Identity = 35/57 (61.40%), Postives = 42/57 (73.68%), Query Frame = 0 

Query: 10 LLIATLACLSFYTGVN--VTYDSNAIIINGERRIIFSGSIHYPRSTEKMWPDLIKKA 65
          +L+  L C S    V   VTYD  A+IING+RRI+ SGSIHYPRST +MWPDLI+KA
Sbjct: 11 ILLGILCCSSLICSVKAIVTYDRKAVIINGQRRILLSGSIHYPRSTPEMWPDLIQKA 67

[Close]
BLAST of ClCG08G009152 vs. TAIR 10
Match: AT4G26140.2 (beta-galactosidase 12 )

HSP 1 Score: 72.8 bits (177), Expect = 1.7e-13
Identity = 35/57 (61.40%), Postives = 42/57 (73.68%), Query Frame = 0 

Query: 10 LLIATLACLSFYTGVN--VTYDSNAIIINGERRIIFSGSIHYPRSTEKMWPDLIKKA 65
          +L+  L C S    V   VTYD  A+IING+RRI+ SGSIHYPRST +MWPDLI+KA
Sbjct: 11 ILLGILCCSSLICSVKAIVTYDRKAVIINGQRRILLSGSIHYPRSTPEMWPDLIQKA 67

[Close]
BLAST of ClCG08G009152 vs. TAIR 10
Match: AT5G56870.1 (beta-galactosidase 4 )

HSP 1 Score: 69.3 bits (168), Expect = 1.8e-12
Identity = 33/57 (57.89%), Postives = 42/57 (73.68%), Query Frame = 0 

Query: 10 LLIATLACLSF--YTGVNVTYDSNAIIINGERRIIFSGSIHYPRSTEKMWPDLIKKA 65
          + +A L CLS       +V+YD  A+IING+RRI+ SGSIHYPRST +MWP LI+KA
Sbjct: 11 IFLAILCCLSLSCIVKASVSYDRKAVIINGQRRILLSGSIHYPRSTPEMWPGLIQKA 67

[Close]
BLAST of ClCG08G009152 vs. TAIR 10
Match: AT3G13750.1 (beta galactosidase 1 )

HSP 1 Score: 68.6 bits (166), Expect = 3.1e-12
Identity = 34/56 (60.71%), Postives = 41/56 (73.21%), Query Frame = 0 

Query: 9  LLLIATLACLSFYTGVNVTYDSNAIIINGERRIIFSGSIHYPRSTEKMWPDLIKKA 65
          L L+  L C       +V+YDS AI ING+RRI+ SGSIHYPRST +MWPDLI+KA
Sbjct: 20 LFLLGFLVC---SVSGSVSYDSRAITINGKRRILISGSIHYPRSTPEMWPDLIRKA 72

The following BLAST results are available for this feature:
BLAST of ClCG08G009152 vs. NCBI nr
Match NameE-valueIdentityDescription
KAA0045403.13.8e-2085.94beta-galactosidase-like [Cucumis melo var. makuwa] >TYK11334.1 beta-galactosidas... [more]
XP_008464891.13.8e-2085.94PREDICTED: beta-galactosidase-like [Cucumis melo][more]
XP_038893262.17.2e-1981.25beta-galactosidase 7-like [Benincasa hispida][more]
XP_008459141.12.1e-1882.81PREDICTED: beta-galactosidase 15-like [Cucumis melo][more]
XP_022961111.11.0e-1776.56beta-galactosidase 7-like [Cucurbita moschata][more]
BLAST of ClCG08G009152 vs. ExPASy Swiss-Prot
Match NameE-valueIdentityDescription
Q9LFA61.4e-1257.14Beta-galactosidase 2 OS=Arabidopsis thaliana OX=3702 GN=BGAL2 PE=2 SV=2[more]
Q67VU72.3e-1261.40Putative beta-galactosidase 10 OS=Oryza sativa subsp. japonica OX=39947 GN=Os06g... [more]
Q9SCV02.3e-1261.40Beta-galactosidase 12 OS=Arabidopsis thaliana OX=3702 GN=BGAL12 PE=2 SV=1[more]
Q8W0A15.2e-1256.14Beta-galactosidase 2 OS=Oryza sativa subsp. japonica OX=39947 GN=Os01g0580200 PE... [more]
P455826.8e-1257.14Beta-galactosidase OS=Asparagus officinalis OX=4686 PE=2 SV=1[more]
BLAST of ClCG08G009152 vs. ExPASy TrEMBL
Match NameE-valueIdentityDescription
A0A5A7TPD91.8e-2085.94Beta-galactosidase OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold807G0... [more]
A0A1S3CP451.8e-2085.94Beta-galactosidase OS=Cucumis melo OX=3656 GN=LOC103502650 PE=3 SV=1[more]
A0A1S3C8Z91.0e-1882.81Beta-galactosidase OS=Cucumis melo OX=3656 GN=LOC103498345 PE=3 SV=1[more]
A0A6J1H9F65.0e-1876.56Beta-galactosidase OS=Cucurbita moschata OX=3662 GN=LOC111461719 PE=3 SV=1[more]
A0A0A0LDH98.6e-1881.54Beta-galactosidase OS=Cucumis sativus OX=3659 GN=Csa_3G550690 PE=3 SV=1[more]
BLAST of ClCG08G009152 vs. TAIR 10
Match NameE-valueIdentityDescription
AT3G52840.19.7e-1457.14beta-galactosidase 2 [more]
AT4G26140.11.7e-1361.40beta-galactosidase 12 [more]
AT4G26140.21.7e-1361.40beta-galactosidase 12 [more]
AT5G56870.11.8e-1257.89beta-galactosidase 4 [more]
AT3G13750.13.1e-1260.71beta galactosidase 1 [more]
InterPro
Analysis Name: InterPro Annotations of Watermelon (Charleston Gray) v2.5
Date Performed: 2022-01-31
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR001944Glycoside hydrolase, family 35PRINTSPR00742GLHYDRLASE35coord: 57..75
score: 27.86
coord: 36..53
score: 66.99
IPR001944Glycoside hydrolase, family 35PANTHERPTHR23421BETA-GALACTOSIDASE RELATEDcoord: 6..65
NoneNo IPR availableGENE3D3.20.20.80Glycosidasescoord: 19..67
e-value: 1.5E-11
score: 46.3
NoneNo IPR availablePANTHERPTHR23421:SF162BETA-GALACTOSIDASEcoord: 6..65
NoneNo IPR availablePROSITEPS51257PROKAR_LIPOPROTEINcoord: 1..17
score: 5.0
IPR031330Glycoside hydrolase 35, catalytic domainPFAMPF01301Glyco_hydro_35coord: 33..64
e-value: 1.2E-8
score: 35.0
IPR017853Glycoside hydrolase superfamilySUPERFAMILY51445(Trans)glycosidasescoord: 22..64

Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
ClCG08G009152.1ClCG08G009152.1mRNA


GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
biological_process GO:0005975 carbohydrate metabolic process
cellular_component GO:0048046 apoplast
cellular_component GO:0005773 vacuole
molecular_function GO:0004565 beta-galactosidase activity
molecular_function GO:0030246 carbohydrate binding
molecular_function GO:0004553 hydrolase activity, hydrolyzing O-glycosyl compounds