ClCG08G005380 (gene) Watermelon (Charleston Gray) v2.5

Overview


Name	ClCG08G005380
Type	gene
Organism	Citrullus lanatus subsp. vulgaris cv. Charleston Gray (Watermelon (Charleston Gray) v2.5)
Description	flavonol synthase/flavanone 3-hydroxylase
Location	CG_Chr08: 17142569 .. 17143925 (-)
RNA-Seq Expression	ClCG08G005380
Synteny	ClCG08G005380

Sequences

The following sequences are available for this feature:

Gene sequence (with intron)

Legend: polypeptideexonCDS

Hold the cursor over a type above to highlight its positions in the sequence below.

ATGACGCACCCAACTGGAAGTTGGTTGAAACCGTATCCTTTGAAACCAAACCTCTCTTTTTTTATTTTGATTATATTACGTTTTGATGGCACTTCAACGCAACCGTCCATAACTCTCCTCATCTCTTTCTTCTCCTCACCAATTTCATCGCACTTTAACACAGAAATGGAGGTCGGGAGAGTCCAGGACATCGCTTCCTCGGTGTTTTTCTCCAACACTCTCCCCGCCGAGTTCATCCGTCCGGAGGCAGAGCAGCCCGGAATCACAACCGTCAAAGGCCACACCCTCGCCGTTCCTGTCATCGATTTCACCAACCCAGATGACGACTCTGTTCTCCGAGCCATCGACGACGCCTCTCGCAATTGGGGCATGTTTCAAATCGTCAACCATCAGATCCCAAATGAAGCAATAACCCATTTGCAGGCCGTCGGTAAGAAATTCTTCGAGCTCCCTCCGGAGGAGAAAGAGGTTTACGCCAAACCCCCTGATTCCAAATCCGTCGAAGGCTATGGAACTAAGCTCCAAAAGGAAATCGATGGAAAGAAGGGTTGGGTCGACCATTTATTCCACATCATTTGGCCTCCTTCTGCCATCAACTACATTTTCTGGCCCAAAAATCCCTGTTCCTACAGGTACGAACATCAAGAAATCCCTCGAAATTTAAATCGTTGAAGGATCGTACATCTTGATTTGTTACTCCGTCTCTGGGTCTGTCTGATATGATACAGGGCAGCGAATGAGGAGTACAGAAAGCACTTACATGGGGTTGTGGAGAAGCTATTCAGATGCCTCTCGTTAGGGCTGGGGCTTGAAGGGCACGAGCTAAAAGAGCGAGCCGGTGGAGATAAATTGGTGTATTTACTGAAAATCAATTACTACCCGCCATGTCCACGCCCTGATTTGGCTCTTGGAGTCCCTGCTCATACCGACATGTCGGCCATAACGATTCTTGTGCCGAATGAAATTCAAGGACTCCAAGCGTTTAGAGATGGGAAGTGGTACGACGTCAGTTACATCCCTAATGCCCTCGTCATCCACATCGGGGATCAAATTGAGGTAAATGTTTTGTTCAACTTCAGACATTTTGATTTTTCTGTTGTGGAAAATGGCTGAATTAAAGTGTACTGTTGTGTTGTGAGGGCAGATTCTGAGTAATGGAAAGTATAAGAGTGTGTTTCATAGGACGACTGTGAACAAGGAGAAGACGAGGATATCGTGGCCGGTGTTTTTGGAGCCGCCGCCGGAGTTTGAAGTTGGGCCTCATCCGAAATTGATCGATGAGGAAAATCCGCCAAAATATAAGACAAAGAAGTACAGTGAATATGCATATTGTAAGCTCAACAAGATTCCCCAGTGA

mRNA sequence

ATGACGCACCCAACTGGAAGTTGGTTGAAACCGTATCCTTTGAAACCAAACCTCTCTTTTTTTATTTTGATTATATTACGTTTTGATGGCACTTCAACGCAACCGTCCATAACTCTCCTCATCTCTTTCTTCTCCTCACCAATTTCATCGCACTTTAACACAGAAATGGAGGTCGGGAGAGTCCAGGACATCGCTTCCTCGGTGTTTTTCTCCAACACTCTCCCCGCCGAGTTCATCCGTCCGGAGGCAGAGCAGCCCGGAATCACAACCGTCAAAGGCCACACCCTCGCCGTTCCTGTCATCGATTTCACCAACCCAGATGACGACTCTGTTCTCCGAGCCATCGACGACGCCTCTCGCAATTGGGGCATGTTTCAAATCGTCAACCATCAGATCCCAAATGAAGCAATAACCCATTTGCAGGCCGTCGGTAAGAAATTCTTCGAGCTCCCTCCGGAGGAGAAAGAGGTTTACGCCAAACCCCCTGATTCCAAATCCGTCGAAGGCTATGGAACTAAGCTCCAAAAGGAAATCGATGGAAAGAAGGGTTGGGTCGACCATTTATTCCACATCATTTGGCCTCCTTCTGCCATCAACTACATTTTCTGGCCCAAAAATCCCTGTTCCTACAGGGCAGCGAATGAGGAGTACAGAAAGCACTTACATGGGGTTGTGGAGAAGCTATTCAGATGCCTCTCGTTAGGGCTGGGGCTTGAAGGGCACGAGCTAAAAGAGCGAGCCGGTGGAGATAAATTGGTGTATTTACTGAAAATCAATTACTACCCGCCATGTCCACGCCCTGATTTGGCTCTTGGAGTCCCTGCTCATACCGACATGTCGGCCATAACGATTCTTGTGCCGAATGAAATTCAAGGACTCCAAGCGTTTAGAGATGGGAAGTGGTACGACGTCAGTTACATCCCTAATGCCCTCGTCATCCACATCGGGGATCAAATTGAGATTCTGAGTAATGGAAAGTATAAGAGTGTGTTTCATAGGACGACTGTGAACAAGGAGAAGACGAGGATATCGTGGCCGGTGTTTTTGGAGCCGCCGCCGGAGTTTGAAGTTGGGCCTCATCCGAAATTGATCGATGAGGAAAATCCGCCAAAATATAAGACAAAGAAGTACAGTGAATATGCATATTGTAAGCTCAACAAGATTCCCCAGTGA

Coding sequence (CDS)

ATGACGCACCCAACTGGAAGTTGGTTGAAACCGTATCCTTTGAAACCAAACCTCTCTTTTTTTATTTTGATTATATTACGTTTTGATGGCACTTCAACGCAACCGTCCATAACTCTCCTCATCTCTTTCTTCTCCTCACCAATTTCATCGCACTTTAACACAGAAATGGAGGTCGGGAGAGTCCAGGACATCGCTTCCTCGGTGTTTTTCTCCAACACTCTCCCCGCCGAGTTCATCCGTCCGGAGGCAGAGCAGCCCGGAATCACAACCGTCAAAGGCCACACCCTCGCCGTTCCTGTCATCGATTTCACCAACCCAGATGACGACTCTGTTCTCCGAGCCATCGACGACGCCTCTCGCAATTGGGGCATGTTTCAAATCGTCAACCATCAGATCCCAAATGAAGCAATAACCCATTTGCAGGCCGTCGGTAAGAAATTCTTCGAGCTCCCTCCGGAGGAGAAAGAGGTTTACGCCAAACCCCCTGATTCCAAATCCGTCGAAGGCTATGGAACTAAGCTCCAAAAGGAAATCGATGGAAAGAAGGGTTGGGTCGACCATTTATTCCACATCATTTGGCCTCCTTCTGCCATCAACTACATTTTCTGGCCCAAAAATCCCTGTTCCTACAGGGCAGCGAATGAGGAGTACAGAAAGCACTTACATGGGGTTGTGGAGAAGCTATTCAGATGCCTCTCGTTAGGGCTGGGGCTTGAAGGGCACGAGCTAAAAGAGCGAGCCGGTGGAGATAAATTGGTGTATTTACTGAAAATCAATTACTACCCGCCATGTCCACGCCCTGATTTGGCTCTTGGAGTCCCTGCTCATACCGACATGTCGGCCATAACGATTCTTGTGCCGAATGAAATTCAAGGACTCCAAGCGTTTAGAGATGGGAAGTGGTACGACGTCAGTTACATCCCTAATGCCCTCGTCATCCACATCGGGGATCAAATTGAGATTCTGAGTAATGGAAAGTATAAGAGTGTGTTTCATAGGACGACTGTGAACAAGGAGAAGACGAGGATATCGTGGCCGGTGTTTTTGGAGCCGCCGCCGGAGTTTGAAGTTGGGCCTCATCCGAAATTGATCGATGAGGAAAATCCGCCAAAATATAAGACAAAGAAGTACAGTGAATATGCATATTGTAAGCTCAACAAGATTCCCCAGTGA

Protein sequence

MTHPTGSWLKPYPLKPNLSFFILIILRFDGTSTQPSITLLISFFSSPISSHFNTEMEVGRVQDIASSVFFSNTLPAEFIRPEAEQPGITTVKGHTLAVPVIDFTNPDDDSVLRAIDDASRNWGMFQIVNHQIPNEAITHLQAVGKKFFELPPEEKEVYAKPPDSKSVEGYGTKLQKEIDGKKGWVDHLFHIIWPPSAINYIFWPKNPCSYRAANEEYRKHLHGVVEKLFRCLSLGLGLEGHELKERAGGDKLVYLLKINYYPPCPRPDLALGVPAHTDMSAITILVPNEIQGLQAFRDGKWYDVSYIPNALVIHIGDQIEILSNGKYKSVFHRTTVNKEKTRISWPVFLEPPPEFEVGPHPKLIDEENPPKYKTKKYSEYAYCKLNKIPQ

Homology

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BLAST of ClCG08G005380 vs. NCBI nr
Match: XP_038885635.1 (flavonol synthase/flavanone 3-hydroxylase [Benincasa hispida])

HSP 1 Score: 657.5 bits (1695), Expect = 7.1e-185
Identity = 307/334 (91.92%), Postives = 323/334 (96.71%), Query Frame = 0


Query: 56  MEVGRVQDIASSVFFSNTLPAEFIRPEAEQPGITTVKGHTLAVPVIDFTNPDDDSVLRAI 115
           MEVGRVQDIASSV +S+TLPAEF+R E EQPGITTVKG  LAVPVIDFT+PDDDSVLRAI
Sbjct: 1   MEVGRVQDIASSVLYSDTLPAEFVRSETEQPGITTVKGQVLAVPVIDFTDPDDDSVLRAI 60

Query: 116 DDASRNWGMFQIVNHQIPNEAITHLQAVGKKFFELPPEEKEVYAKPPDSKSVEGYGTKLQ 175
           DDASRNWGMFQI+NH+IPNEAITHLQAVGK+FFELPPEEKEVYAKPP SKSVEGYGTKLQ
Sbjct: 61  DDASRNWGMFQIINHEIPNEAITHLQAVGKEFFELPPEEKEVYAKPPGSKSVEGYGTKLQ 120

Query: 176 KEIDGKKGWVDHLFHIIWPPSAINYIFWPKNPCSYRAANEEYRKHLHGVVEKLFRCLSLG 235
           KEIDGKKGWVDHLFHIIWPPSAINY FWP+NP SYRAANEEY KHLHGVVEKLFR LSLG
Sbjct: 121 KEIDGKKGWVDHLFHIIWPPSAINYNFWPQNPPSYRAANEEYSKHLHGVVEKLFRSLSLG 180

Query: 236 LGLEGHELKERAGGDKLVYLLKINYYPPCPRPDLALGVPAHTDMSAITILVPNEIQGLQA 295
           LGLEGHELKERAGG++LVYLLKINYYPPCPRPDLALGVPAHTDMSAITILVPNE+QGLQA
Sbjct: 181 LGLEGHELKERAGGEELVYLLKINYYPPCPRPDLALGVPAHTDMSAITILVPNEVQGLQA 240

Query: 296 FRDGKWYDVSYIPNALVIHIGDQIEILSNGKYKSVFHRTTVNKEKTRISWPVFLEPPPEF 355
           FRDG+WYDV+YIPNALVIHIGDQ+EILSNGKYKSVFHRT VNKEKTRISWPVFLEPPPEF
Sbjct: 241 FRDGQWYDVNYIPNALVIHIGDQMEILSNGKYKSVFHRTRVNKEKTRISWPVFLEPPPEF 300

Query: 356 EVGPHPKLIDEENPPKYKTKKYSEYAYCKLNKIP 390
           EVGPHPKL+DE+NPPKYKTKKYSEYAYCKLNKIP
Sbjct: 301 EVGPHPKLVDEKNPPKYKTKKYSEYAYCKLNKIP 334

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BLAST of ClCG08G005380 vs. NCBI nr
Match: XP_008445671.1 (PREDICTED: flavonol synthase/flavanone 3-hydroxylase [Cucumis melo] >KAA0063921.1 flavonol synthase/flavanone 3-hydroxylase [Cucumis melo var. makuwa] >TYK11311.1 flavonol synthase/flavanone 3-hydroxylase [Cucumis melo var. makuwa])

HSP 1 Score: 645.2 bits (1663), Expect = 3.6e-181
Identity = 299/335 (89.25%), Postives = 318/335 (94.93%), Query Frame = 0


Query: 56  MEVGRVQDIASSVFFSNTLPAEFIRPEAEQPGITTVKGHTLAVPVIDFTNPDDDSVLRAI 115
           M+VGRVQ++ASS+ FS TLP+EF+RPE EQPGITTVKG  LAVPVIDFTNPDDDSVL AI
Sbjct: 1   MDVGRVQEVASSLLFSGTLPSEFVRPETEQPGITTVKGQVLAVPVIDFTNPDDDSVLTAI 60

Query: 116 DDASRNWGMFQIVNHQIPNEAITHLQAVGKKFFELPPEEKEVYAKPPDSKSVEGYGTKLQ 175
            DAS NWGMFQI+NHQIPNEAITHLQAVGK+FFELPPEEKE+YAKPP SKSVEGYGTKL+
Sbjct: 61  HDASLNWGMFQIINHQIPNEAITHLQAVGKEFFELPPEEKEIYAKPPASKSVEGYGTKLE 120

Query: 176 KEIDGKKGWVDHLFHIIWPPSAINYIFWPKNPCSYRAANEEYRKHLHGVVEKLFRCLSLG 235
           KEIDGK+GWVDHLFHIIWPPS+INY FWP+NP SYR ANEEYRK+LHGVVEKLFR LSLG
Sbjct: 121 KEIDGKRGWVDHLFHIIWPPSSINYNFWPQNPPSYRVANEEYRKNLHGVVEKLFRSLSLG 180

Query: 236 LGLEGHELKERAGGDKLVYLLKINYYPPCPRPDLALGVPAHTDMSAITILVPNEIQGLQA 295
           LGLEGHELKER GGD+LVYLLKINYYPPCPRPDLALGVPAHTDMSA+TILVPNE+QGLQA
Sbjct: 181 LGLEGHELKERVGGDELVYLLKINYYPPCPRPDLALGVPAHTDMSAVTILVPNEVQGLQA 240

Query: 296 FRDGKWYDVSYIPNALVIHIGDQIEILSNGKYKSVFHRTTVNKEKTRISWPVFLEPPPEF 355
           FRDG WYDV+YIPNALVIHIGDQIEILSNGKYKSVFHRTTVNKEKTRISWPVFLEPPPEF
Sbjct: 241 FRDGHWYDVNYIPNALVIHIGDQIEILSNGKYKSVFHRTTVNKEKTRISWPVFLEPPPEF 300

Query: 356 EVGPHPKLIDEENPPKYKTKKYSEYAYCKLNKIPQ 391
            VGPHPKL+DEENP KYKTKKYSEYAYCKLNKIPQ
Sbjct: 301 VVGPHPKLVDEENPAKYKTKKYSEYAYCKLNKIPQ 335

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BLAST of ClCG08G005380 vs. NCBI nr
Match: XP_023551148.1 (flavonol synthase/flavanone 3-hydroxylase-like [Cucurbita pepo subsp. pepo])

HSP 1 Score: 639.0 bits (1647), Expect = 2.6e-179
Identity = 295/335 (88.06%), Postives = 314/335 (93.73%), Query Frame = 0


Query: 56  MEVGRVQDIASSVFFSNTLPAEFIRPEAEQPGITTVKGHTLAVPVIDFTNPDDDSVLRAI 115
           MEVGRVQDIASS  FS +LPAEF+RP+ EQPGITTVKG  LAVPVIDF NPD+DSV+RAI
Sbjct: 1   MEVGRVQDIASSALFSGSLPAEFVRPDTEQPGITTVKGQLLAVPVIDFKNPDEDSVIRAI 60

Query: 116 DDASRNWGMFQIVNHQIPNEAITHLQAVGKKFFELPPEEKEVYAKPPDSKSVEGYGTKLQ 175
           DDASRNWGMFQI+NH IPNEAI HLQAVGK FFELPPEEKEVYAKPPDSKSVEGYGTKLQ
Sbjct: 61  DDASRNWGMFQIINHDIPNEAIAHLQAVGKAFFELPPEEKEVYAKPPDSKSVEGYGTKLQ 120

Query: 176 KEIDGKKGWVDHLFHIIWPPSAINYIFWPKNPCSYRAANEEYRKHLHGVVEKLFRCLSLG 235
           KE+DGKKGWVDHLFHIIWPPSAIN+ FWP+NP SYRAANEEYRKHLHGVV+KLFR LSLG
Sbjct: 121 KEVDGKKGWVDHLFHIIWPPSAINFKFWPQNPPSYRAANEEYRKHLHGVVDKLFRSLSLG 180

Query: 236 LGLEGHELKERAGGDKLVYLLKINYYPPCPRPDLALGVPAHTDMSAITILVPNEIQGLQA 295
           LGLEGHELKE AGGDKLVYLLKINYYPPCP PDLALGVPAHTDMS ITILVPN++QGLQA
Sbjct: 181 LGLEGHELKESAGGDKLVYLLKINYYPPCPCPDLALGVPAHTDMSTITILVPNDVQGLQA 240

Query: 296 FRDGKWYDVSYIPNALVIHIGDQIEILSNGKYKSVFHRTTVNKEKTRISWPVFLEPPPEF 355
           FRDG WY+V+YIPNALVIHIGDQIEILSNGKYKSV HRTTV K++ RISWPVFLEPPPEF
Sbjct: 241 FRDGHWYNVNYIPNALVIHIGDQIEILSNGKYKSVLHRTTVKKDRVRISWPVFLEPPPEF 300

Query: 356 EVGPHPKLIDEENPPKYKTKKYSEYAYCKLNKIPQ 391
           EVGPHPKL+D++NPPKYKTKKY EYAYCKLNKIPQ
Sbjct: 301 EVGPHPKLVDQDNPPKYKTKKYGEYAYCKLNKIPQ 335

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BLAST of ClCG08G005380 vs. NCBI nr
Match: XP_004140862.1 (flavonol synthase/flavanone 3-hydroxylase [Cucumis sativus] >KGN45965.1 hypothetical protein Csa_005329 [Cucumis sativus])

HSP 1 Score: 638.3 bits (1645), Expect = 4.4e-179
Identity = 296/335 (88.36%), Postives = 316/335 (94.33%), Query Frame = 0


Query: 56  MEVGRVQDIASSVFFSNTLPAEFIRPEAEQPGITTVKGHTLAVPVIDFTNPDDDSVLRAI 115
           MEVGRVQ++ASS+ FS TLP++F+RP+ EQPGITTVKG  LAVPVIDFTNPDDDSVL AI
Sbjct: 1   MEVGRVQEVASSLLFSGTLPSQFVRPDTEQPGITTVKGQVLAVPVIDFTNPDDDSVLTAI 60

Query: 116 DDASRNWGMFQIVNHQIPNEAITHLQAVGKKFFELPPEEKEVYAKPPDSKSVEGYGTKLQ 175
            DAS NWGMFQI+NHQIPNEAITHLQAVGK+FF+LPPE KE+YAKPP SKSVEGYGT LQ
Sbjct: 61  HDASLNWGMFQIINHQIPNEAITHLQAVGKEFFQLPPEMKEIYAKPPASKSVEGYGTNLQ 120

Query: 176 KEIDGKKGWVDHLFHIIWPPSAINYIFWPKNPCSYRAANEEYRKHLHGVVEKLFRCLSLG 235
           KEIDGK+GWVDHLFHIIWPPS+INY FWP+NP SYRAANEEYRKHLHGVVEKLFR LSLG
Sbjct: 121 KEIDGKRGWVDHLFHIIWPPSSINYNFWPQNPPSYRAANEEYRKHLHGVVEKLFRSLSLG 180

Query: 236 LGLEGHELKERAGGDKLVYLLKINYYPPCPRPDLALGVPAHTDMSAITILVPNEIQGLQA 295
           LGLEGHELKER GGD+LVYLLKINYYPPCPRPDLALGVPAHTDMSAITILVPNE+QGLQA
Sbjct: 181 LGLEGHELKERVGGDELVYLLKINYYPPCPRPDLALGVPAHTDMSAITILVPNEVQGLQA 240

Query: 296 FRDGKWYDVSYIPNALVIHIGDQIEILSNGKYKSVFHRTTVNKEKTRISWPVFLEPPPEF 355
           FRDG WYDV+YIPNALVIHIGDQIEILSNGKYKSV HRTTVNKEKTRISWPVFLEPPPEF
Sbjct: 241 FRDGHWYDVNYIPNALVIHIGDQIEILSNGKYKSVLHRTTVNKEKTRISWPVFLEPPPEF 300

Query: 356 EVGPHPKLIDEENPPKYKTKKYSEYAYCKLNKIPQ 391
           EVGPHPKL+DE+ P +YKTKKYSEYAYCKLNKIPQ
Sbjct: 301 EVGPHPKLVDEKIPARYKTKKYSEYAYCKLNKIPQ 335

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BLAST of ClCG08G005380 vs. NCBI nr
Match: KAG7016505.1 (Flavonol synthase/flavanone 3-hydroxylase, partial [Cucurbita argyrosperma subsp. argyrosperma])

HSP 1 Score: 637.9 bits (1644), Expect = 5.8e-179
Identity = 294/335 (87.76%), Postives = 314/335 (93.73%), Query Frame = 0


Query: 56  MEVGRVQDIASSVFFSNTLPAEFIRPEAEQPGITTVKGHTLAVPVIDFTNPDDDSVLRAI 115
           MEVGRVQDIASS  FS +LPAEF+RP+ EQPGITTVKG  LAVPVIDF NPD+DSV++AI
Sbjct: 1   MEVGRVQDIASSALFSGSLPAEFVRPDTEQPGITTVKGQLLAVPVIDFKNPDEDSVIKAI 60

Query: 116 DDASRNWGMFQIVNHQIPNEAITHLQAVGKKFFELPPEEKEVYAKPPDSKSVEGYGTKLQ 175
           DDASRNWGMFQI+NH IPNEAI HLQAVGK FFELPPEEKEVYAKPPDSKSVEGYGTKLQ
Sbjct: 61  DDASRNWGMFQIINHDIPNEAIAHLQAVGKAFFELPPEEKEVYAKPPDSKSVEGYGTKLQ 120

Query: 176 KEIDGKKGWVDHLFHIIWPPSAINYIFWPKNPCSYRAANEEYRKHLHGVVEKLFRCLSLG 235
           KE+DGKKGWVDHLFHIIWPPSAIN+ FWP+NP SYRAANEEYRKHLHGVV+KLFR LSLG
Sbjct: 121 KEVDGKKGWVDHLFHIIWPPSAINFKFWPQNPPSYRAANEEYRKHLHGVVDKLFRSLSLG 180

Query: 236 LGLEGHELKERAGGDKLVYLLKINYYPPCPRPDLALGVPAHTDMSAITILVPNEIQGLQA 295
           LGLEGHELKE AGGDKLVYLLKINYYPPCP PDLALGVPAHTDMS ITILVPN++QGLQA
Sbjct: 181 LGLEGHELKESAGGDKLVYLLKINYYPPCPCPDLALGVPAHTDMSTITILVPNDVQGLQA 240

Query: 296 FRDGKWYDVSYIPNALVIHIGDQIEILSNGKYKSVFHRTTVNKEKTRISWPVFLEPPPEF 355
           FRDG WY+V+YIPNALVIHIGDQIEILSNGKYKSV HRTTV K++ RISWPVFLEPPPEF
Sbjct: 241 FRDGHWYNVNYIPNALVIHIGDQIEILSNGKYKSVLHRTTVKKDRVRISWPVFLEPPPEF 300

Query: 356 EVGPHPKLIDEENPPKYKTKKYSEYAYCKLNKIPQ 391
           EVGPHPKL+D++NPPKYKTKKY EYAYCKLNKIPQ
Sbjct: 301 EVGPHPKLVDQDNPPKYKTKKYGEYAYCKLNKIPQ 335

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BLAST of ClCG08G005380 vs. ExPASy Swiss-Prot
Match: Q9XHG2 (Flavonol synthase/flavanone 3-hydroxylase OS=Malus domestica OX=3750 GN=FLS PE=2 SV=1)

HSP 1 Score: 516.5 bits (1329), Expect = 2.6e-145
Identity = 239/322 (74.22%), Postives = 272/322 (84.47%), Query Frame = 0


Query: 73  TLPAEFIRPEAEQPGITTVKGHTLAVPVIDFTNPDDDSVLRAIDDASRNWGMFQIVNHQI 132
           T+PAEFIR E EQPGITTV G  L VP+IDF++PD++ ++  I +AS NWGM+QIVNH I
Sbjct: 16  TIPAEFIRSENEQPGITTVHGKVLEVPIIDFSDPDEEKLIVQITEASSNWGMYQIVNHDI 75

Query: 133 PNEAITHLQAVGKKFFELPPEEKEVYAKPPDSKSVEGYGTKLQKEID----GKKGWVDHL 192
           P+E I+ LQAVGK+FFELP EEKE YAKPPDS S+EGYGTKL KEI      KKGWVD+L
Sbjct: 76  PSEVISKLQAVGKEFFELPQEEKEAYAKPPDSASIEGYGTKLFKEISEGDTTKKGWVDNL 135

Query: 193 FHIIWPPSAINYIFWPKNPCSYRAANEEYRKHLHGVVEKLFRCLSLGLGLEGHELKERAG 252
           F+ IWPPS +NY FWPKNP SYR ANEEY KHLH VVEKLFR LSLGLGLEG ELK+ AG
Sbjct: 136 FNKIWPPSVVNYQFWPKNPPSYREANEEYAKHLHNVVEKLFRLLSLGLGLEGQELKKAAG 195

Query: 253 GDKLVYLLKINYYPPCPRPDLALGVPAHTDMSAITILVPNEIQGLQAFRDGKWYDVSYIP 312
           GD L YLLKINYYPPCPRPDLALGV AHTDMS +TILVPN++QGLQA +DG+WYDV YIP
Sbjct: 196 GDNLEYLLKINYYPPCPRPDLALGVVAHTDMSTVTILVPNDVQGLQACKDGRWYDVKYIP 255

Query: 313 NALVIHIGDQIEILSNGKYKSVFHRTTVNKEKTRISWPVFLEPPPEFEVGPHPKLIDEEN 372
           NALVIHIGDQ+EI+SNGKY SV HRTTVNK+KTRISWPVFLEPP +  VGPHP+L++  N
Sbjct: 256 NALVIHIGDQMEIMSNGKYTSVLHRTTVNKDKTRISWPVFLEPPADHVVGPHPQLVNAVN 315

Query: 373 PPKYKTKKYSEYAYCKLNKIPQ 391
            PKYKTKKY +Y YCK+NK+PQ
Sbjct: 316 QPKYKTKKYGDYVYCKINKLPQ 337

[Close]

BLAST of ClCG08G005380 vs. ExPASy Swiss-Prot
Match: Q07512 (Flavonol synthase/flavanone 3-hydroxylase OS=Petunia hybrida OX=4102 GN=FL PE=2 SV=1)

HSP 1 Score: 516.2 bits (1328), Expect = 3.3e-145
Identity = 236/336 (70.24%), Postives = 280/336 (83.33%), Query Frame = 0


Query: 56  MEVGRVQDIASSVFFSNTLPAEFIRPEAEQPGITTVKGHTLAVPVIDFTNPDDDSVLRAI 115
           MEV RVQ IAS     +T+P+E+IR E EQP  TT+ G  L VPVID  +PD++ +++ I
Sbjct: 13  MEVARVQAIASLSKCMDTIPSEYIRSENEQPAATTLHGVVLQVPVIDLRDPDENKMVKLI 72

Query: 116 DDASRNWGMFQIVNHQIPNEAITHLQAVGKKFFE-LPPEEKEVYAKPPDSKSVEGYGTKL 175
            DAS+ WG+FQ++NH IP+EAI  LQ VGK+FFE +P EEKE+ AK P S  +EGYGT L
Sbjct: 73  ADASKEWGIFQLINHGIPDEAIADLQKVGKEFFEHVPQEEKELIAKTPGSNDIEGYGTSL 132

Query: 176 QKEIDGKKGWVDHLFHIIWPPSAINYIFWPKNPCSYRAANEEYRKHLHGVVEKLFRCLSL 235
           QKE++GKKGWVDHLFH IWPPSA+NY +WPKNP SYR ANEEY K +  VV+++F+ LSL
Sbjct: 133 QKEVEGKKGWVDHLFHKIWPPSAVNYRYWPKNPPSYREANEEYGKRMREVVDRIFKSLSL 192

Query: 236 GLGLEGHELKERAGGDKLVYLLKINYYPPCPRPDLALGVPAHTDMSAITILVPNEIQGLQ 295
           GLGLEGHE+ E AGGD++VYLLKINYYPPCPRPDLALGV AHTDMS ITILVPNE+QGLQ
Sbjct: 193 GLGLEGHEMIEAAGGDEIVYLLKINYYPPCPRPDLALGVVAHTDMSYITILVPNEVQGLQ 252

Query: 296 AFRDGKWYDVSYIPNALVIHIGDQIEILSNGKYKSVFHRTTVNKEKTRISWPVFLEPPPE 355
            F+DG WYDV YIPNAL++HIGDQ+EILSNGKYKSV+HRTTVNK+KTR+SWPVFLEPP E
Sbjct: 253 VFKDGHWYDVKYIPNALIVHIGDQVEILSNGKYKSVYHRTTVNKDKTRMSWPVFLEPPSE 312

Query: 356 FEVGPHPKLIDEENPPKYKTKKYSEYAYCKLNKIPQ 391
            EVGP PKL+ E NPPK+KTKKY +Y YCKLNK+PQ
Sbjct: 313 HEVGPIPKLLSEANPPKFKTKKYKDYVYCKLNKLPQ 348

[Close]

BLAST of ClCG08G005380 vs. ExPASy Swiss-Prot
Match: Q9M547 (Flavonol synthase/flavanone 3-hydroxylase OS=Eustoma exaltatum subsp. russellianum OX=52518 GN=FLS PE=2 SV=1)

HSP 1 Score: 510.0 bits (1312), Expect = 2.4e-143
Identity = 236/335 (70.45%), Postives = 278/335 (82.99%), Query Frame = 0


Query: 56  MEVGRVQDIASSVFFSNTLPAEFIRPEAEQPGITTVKGHTLAVPVIDFTNPDDDSVLRAI 115
           MEV RVQ+IAS     +T+PAE+IR E EQP I+TV G  L VPVID ++ D+  ++  +
Sbjct: 1   MEVQRVQEIASLSKVIDTIPAEYIRSENEQPVISTVHGVVLEVPVIDLSDSDEKKIVGLV 60

Query: 116 DDASRNWGMFQIVNHQIPNEAITHLQAVGKKFFELPPEEKEVYAKPPDSKSVEGYGTKLQ 175
            +AS+ WG+FQ+VNH IPNE I  LQ VGK FFELP EEKE+ AKP  S+S+EGYGT+LQ
Sbjct: 61  SEASKEWGIFQVVNHGIPNEVIRKLQEVGKHFFELPQEEKELIAKPEGSQSIEGYGTRLQ 120

Query: 176 KEIDGKKGWVDHLFHIIWPPSAINYIFWPKNPCSYRAANEEYRKHLHGVVEKLFRCLSLG 235
           KE+DGKKGWVDHLFH IWPPSAINY FWPKNP +YR ANEEY K L  VV+ LF+ LSLG
Sbjct: 121 KEVDGKKGWVDHLFHKIWPPSAINYQFWPKNPPAYREANEEYAKRLQLVVDNLFKYLSLG 180

Query: 236 LGLEGHELKERAGGDKLVYLLKINYYPPCPRPDLALGVPAHTDMSAITILVPNEIQGLQA 295
           L LE +  K+ AGGD LVYL+KINYYPPCPRPDLALGV AHTDMSAIT+LVPNE+ GLQ 
Sbjct: 181 LDLEPNSFKDGAGGDDLVYLMKINYYPPCPRPDLALGV-AHTDMSAITVLVPNEVPGLQV 240

Query: 296 FRDGKWYDVSYIPNALVIHIGDQIEILSNGKYKSVFHRTTVNKEKTRISWPVFLEPPPEF 355
           ++DG WYD  YIPNAL++HIGDQ+EI+SNGKYKSV+HRTTVNKEKTR+SWPVFLEPPP+ 
Sbjct: 241 YKDGHWYDCKYIPNALIVHIGDQVEIMSNGKYKSVYHRTTVNKEKTRMSWPVFLEPPPDH 300

Query: 356 EVGPHPKLIDEENPPKYKTKKYSEYAYCKLNKIPQ 391
           EVGP PKL++EENP K+KTKKY +YAYCKLNK+PQ
Sbjct: 301 EVGPIPKLVNEENPAKFKTKKYKDYAYCKLNKLPQ 334

[Close]

BLAST of ClCG08G005380 vs. ExPASy Swiss-Prot
Match: Q9ZWQ9 (Flavonol synthase/flavanone 3-hydroxylase OS=Citrus unshiu OX=55188 GN=FLS PE=1 SV=1)

HSP 1 Score: 504.6 bits (1298), Expect = 1.0e-141
Identity = 230/335 (68.66%), Postives = 274/335 (81.79%), Query Frame = 0


Query: 56  MEVGRVQDIASSVFFSNTLPAEFIRPEAEQPGITTVKGHTLAVPVIDFTNPDDDSVLRAI 115
           MEV RVQ IAS    + T+PAEFIRPE EQP  TT  G    +P ID  +P  D ++R+I
Sbjct: 1   MEVERVQAIASLSHSNGTIPAEFIRPEKEQPASTTYHGPAPEIPTIDLDDPVQDRLVRSI 60

Query: 116 DDASRNWGMFQIVNHQIPNEAITHLQAVGKKFFELPPEEKEVYAKPPDSKSVEGYGTKLQ 175
            +ASR WG+FQ+ NH IP++ I  LQAVGK+FFELP EEKEVY++P D+K V+GYGTKLQ
Sbjct: 61  AEASREWGIFQVTNHGIPSDLICKLQAVGKEFFELPQEEKEVYSRPADAKDVQGYGTKLQ 120

Query: 176 KEIDGKKGWVDHLFHIIWPPSAINYIFWPKNPCSYRAANEEYRKHLHGVVEKLFRCLSLG 235
           KE++GKK WVDHLFH +WPPS+INY FWPKNP SYRA NEEY K++  VV+KLF  LSLG
Sbjct: 121 KEVEGKKSWVDHLFHRVWPPSSINYRFWPKNPPSYRAVNEEYAKYMREVVDKLFTYLSLG 180

Query: 236 LGLEGHELKERAGGDKLVYLLKINYYPPCPRPDLALGVPAHTDMSAITILVPNEIQGLQA 295
           LG+EG  LKE AGGD + Y+LKINYYPPCPRPDLALGV AHTD+SA+T+LVPNE+ GLQ 
Sbjct: 181 LGVEGGVLKEAAGGDDIEYMLKINYYPPCPRPDLALGVVAHTDLSALTVLVPNEVPGLQV 240

Query: 296 FRDGKWYDVSYIPNALVIHIGDQIEILSNGKYKSVFHRTTVNKEKTRISWPVFLEPPPEF 355
           F+D +W D  YIPNALVIHIGDQIEILSNGKYK+V HRTTVNK+KTR+SWPVFLEPP + 
Sbjct: 241 FKDDRWIDAKYIPNALVIHIGDQIEILSNGKYKAVLHRTTVNKDKTRMSWPVFLEPPADT 300

Query: 356 EVGPHPKLIDEENPPKYKTKKYSEYAYCKLNKIPQ 391
            VGP P+L+D+ENPPKYK KK+ +Y+YCKLNK+PQ
Sbjct: 301 VVGPLPQLVDDENPPKYKAKKFKDYSYCKLNKLPQ 335

[Close]

BLAST of ClCG08G005380 vs. ExPASy Swiss-Prot
Match: Q7XZQ6 (Flavonol synthase/flavanone 3-hydroxylase OS=Petroselinum crispum OX=4043 GN=FLS PE=1 SV=1)

HSP 1 Score: 498.0 bits (1281), Expect = 9.4e-140
Identity = 232/337 (68.84%), Postives = 274/337 (81.31%), Query Frame = 0


Query: 56  MEVGRVQDIASSVFFSNTLPAEFIRPEAEQPGITTVKGHTLAVPVIDF--TNPDDDSVLR 115
           MEV RVQ I+      +T+P+E+IR E+EQP +TT++G  L VPVID   +N  +++++ 
Sbjct: 1   MEVERVQAISKMSRCMDTIPSEYIRSESEQPAVTTMQGVVLQVPVIDLGSSNNTEENLVE 60

Query: 116 AIDDASRNWGMFQIVNHQIPNEAITHLQAVGKKFFELPPEEKEVYAKPPDSKSVEGYGTK 175
            I +ASR WG+FQ+VNH IP++AI  LQ VGK+FFELP +EKEV AKP   + VEGYGTK
Sbjct: 61  LIAEASREWGIFQVVNHGIPDDAIAKLQKVGKEFFELPQQEKEVIAKPEGYQGVEGYGTK 120

Query: 176 LQKEIDGKKGWVDHLFHIIWPPSAINYIFWPKNPCSYRAANEEYRKHLHGVVEKLFRCLS 235
           LQKE+ GKKGWVDHLFHIIWP SA+NY FWP NP  YR ANEEY   L GVV+KLF  LS
Sbjct: 121 LQKELGGKKGWVDHLFHIIWPKSAVNYNFWPNNPPLYREANEEYAVALRGVVDKLFEALS 180

Query: 236 LGLGLEGHELKERAGGDKLVYLLKINYYPPCPRPDLALGVPAHTDMSAITILVPNEIQGL 295
           LG+GLE HELK+ +GGD L+Y+LKINYYPPCPRPDLALGV AHTDMSAITILVPNE+QGL
Sbjct: 181 LGIGLEKHELKKASGGDDLIYMLKINYYPPCPRPDLALGVVAHTDMSAITILVPNEVQGL 240

Query: 296 QAFRDGKWYDVSYIPNALVIHIGDQIEILSNGKYKSVFHRTTVNKEKTRISWPVFLEPPP 355
           Q  +D  WYDV YIPNAL+IHIGDQIEI+SNGKYKSV+HRTTVNK+KTR+SWPVFLEPPP
Sbjct: 241 QVHKDDHWYDVKYIPNALIIHIGDQIEIMSNGKYKSVYHRTTVNKDKTRMSWPVFLEPPP 300

Query: 356 EFEVGPHPKLIDEENPPKYKTKKYSEYAYCKLNKIPQ 391
           E   GP  KLI +ENP K+KTKKY +Y YCKLNK+PQ
Sbjct: 301 ELLTGPISKLITDENPAKFKTKKYKDYVYCKLNKLPQ 337

[Close]

BLAST of ClCG08G005380 vs. ExPASy TrEMBL
Match: A0A5D3CHN5 (Flavonol synthase/flavanone 3-hydroxylase OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold1827G00220 PE=3 SV=1)

HSP 1 Score: 645.2 bits (1663), Expect = 1.8e-181
Identity = 299/335 (89.25%), Postives = 318/335 (94.93%), Query Frame = 0


Query: 56  MEVGRVQDIASSVFFSNTLPAEFIRPEAEQPGITTVKGHTLAVPVIDFTNPDDDSVLRAI 115
           M+VGRVQ++ASS+ FS TLP+EF+RPE EQPGITTVKG  LAVPVIDFTNPDDDSVL AI
Sbjct: 1   MDVGRVQEVASSLLFSGTLPSEFVRPETEQPGITTVKGQVLAVPVIDFTNPDDDSVLTAI 60

Query: 116 DDASRNWGMFQIVNHQIPNEAITHLQAVGKKFFELPPEEKEVYAKPPDSKSVEGYGTKLQ 175
            DAS NWGMFQI+NHQIPNEAITHLQAVGK+FFELPPEEKE+YAKPP SKSVEGYGTKL+
Sbjct: 61  HDASLNWGMFQIINHQIPNEAITHLQAVGKEFFELPPEEKEIYAKPPASKSVEGYGTKLE 120

Query: 176 KEIDGKKGWVDHLFHIIWPPSAINYIFWPKNPCSYRAANEEYRKHLHGVVEKLFRCLSLG 235
           KEIDGK+GWVDHLFHIIWPPS+INY FWP+NP SYR ANEEYRK+LHGVVEKLFR LSLG
Sbjct: 121 KEIDGKRGWVDHLFHIIWPPSSINYNFWPQNPPSYRVANEEYRKNLHGVVEKLFRSLSLG 180

Query: 236 LGLEGHELKERAGGDKLVYLLKINYYPPCPRPDLALGVPAHTDMSAITILVPNEIQGLQA 295
           LGLEGHELKER GGD+LVYLLKINYYPPCPRPDLALGVPAHTDMSA+TILVPNE+QGLQA
Sbjct: 181 LGLEGHELKERVGGDELVYLLKINYYPPCPRPDLALGVPAHTDMSAVTILVPNEVQGLQA 240

Query: 296 FRDGKWYDVSYIPNALVIHIGDQIEILSNGKYKSVFHRTTVNKEKTRISWPVFLEPPPEF 355
           FRDG WYDV+YIPNALVIHIGDQIEILSNGKYKSVFHRTTVNKEKTRISWPVFLEPPPEF
Sbjct: 241 FRDGHWYDVNYIPNALVIHIGDQIEILSNGKYKSVFHRTTVNKEKTRISWPVFLEPPPEF 300

Query: 356 EVGPHPKLIDEENPPKYKTKKYSEYAYCKLNKIPQ 391
            VGPHPKL+DEENP KYKTKKYSEYAYCKLNKIPQ
Sbjct: 301 VVGPHPKLVDEENPAKYKTKKYSEYAYCKLNKIPQ 335

[Close]

BLAST of ClCG08G005380 vs. ExPASy TrEMBL
Match: A0A1S3BCS7 (flavonol synthase/flavanone 3-hydroxylase OS=Cucumis melo OX=3656 GN=LOC103488630 PE=3 SV=1)

HSP 1 Score: 645.2 bits (1663), Expect = 1.8e-181
Identity = 299/335 (89.25%), Postives = 318/335 (94.93%), Query Frame = 0


Query: 56  MEVGRVQDIASSVFFSNTLPAEFIRPEAEQPGITTVKGHTLAVPVIDFTNPDDDSVLRAI 115
           M+VGRVQ++ASS+ FS TLP+EF+RPE EQPGITTVKG  LAVPVIDFTNPDDDSVL AI
Sbjct: 1   MDVGRVQEVASSLLFSGTLPSEFVRPETEQPGITTVKGQVLAVPVIDFTNPDDDSVLTAI 60

Query: 116 DDASRNWGMFQIVNHQIPNEAITHLQAVGKKFFELPPEEKEVYAKPPDSKSVEGYGTKLQ 175
            DAS NWGMFQI+NHQIPNEAITHLQAVGK+FFELPPEEKE+YAKPP SKSVEGYGTKL+
Sbjct: 61  HDASLNWGMFQIINHQIPNEAITHLQAVGKEFFELPPEEKEIYAKPPASKSVEGYGTKLE 120

Query: 176 KEIDGKKGWVDHLFHIIWPPSAINYIFWPKNPCSYRAANEEYRKHLHGVVEKLFRCLSLG 235
           KEIDGK+GWVDHLFHIIWPPS+INY FWP+NP SYR ANEEYRK+LHGVVEKLFR LSLG
Sbjct: 121 KEIDGKRGWVDHLFHIIWPPSSINYNFWPQNPPSYRVANEEYRKNLHGVVEKLFRSLSLG 180

Query: 236 LGLEGHELKERAGGDKLVYLLKINYYPPCPRPDLALGVPAHTDMSAITILVPNEIQGLQA 295
           LGLEGHELKER GGD+LVYLLKINYYPPCPRPDLALGVPAHTDMSA+TILVPNE+QGLQA
Sbjct: 181 LGLEGHELKERVGGDELVYLLKINYYPPCPRPDLALGVPAHTDMSAVTILVPNEVQGLQA 240

Query: 296 FRDGKWYDVSYIPNALVIHIGDQIEILSNGKYKSVFHRTTVNKEKTRISWPVFLEPPPEF 355
           FRDG WYDV+YIPNALVIHIGDQIEILSNGKYKSVFHRTTVNKEKTRISWPVFLEPPPEF
Sbjct: 241 FRDGHWYDVNYIPNALVIHIGDQIEILSNGKYKSVFHRTTVNKEKTRISWPVFLEPPPEF 300

Query: 356 EVGPHPKLIDEENPPKYKTKKYSEYAYCKLNKIPQ 391
            VGPHPKL+DEENP KYKTKKYSEYAYCKLNKIPQ
Sbjct: 301 VVGPHPKLVDEENPAKYKTKKYSEYAYCKLNKIPQ 335

[Close]

BLAST of ClCG08G005380 vs. ExPASy TrEMBL
Match: A0A0A0K885 (Fe2OG dioxygenase domain-containing protein OS=Cucumis sativus OX=3659 GN=Csa_6G040540 PE=3 SV=1)

HSP 1 Score: 638.3 bits (1645), Expect = 2.1e-179
Identity = 296/335 (88.36%), Postives = 316/335 (94.33%), Query Frame = 0


Query: 56  MEVGRVQDIASSVFFSNTLPAEFIRPEAEQPGITTVKGHTLAVPVIDFTNPDDDSVLRAI 115
           MEVGRVQ++ASS+ FS TLP++F+RP+ EQPGITTVKG  LAVPVIDFTNPDDDSVL AI
Sbjct: 1   MEVGRVQEVASSLLFSGTLPSQFVRPDTEQPGITTVKGQVLAVPVIDFTNPDDDSVLTAI 60

Query: 116 DDASRNWGMFQIVNHQIPNEAITHLQAVGKKFFELPPEEKEVYAKPPDSKSVEGYGTKLQ 175
            DAS NWGMFQI+NHQIPNEAITHLQAVGK+FF+LPPE KE+YAKPP SKSVEGYGT LQ
Sbjct: 61  HDASLNWGMFQIINHQIPNEAITHLQAVGKEFFQLPPEMKEIYAKPPASKSVEGYGTNLQ 120

Query: 176 KEIDGKKGWVDHLFHIIWPPSAINYIFWPKNPCSYRAANEEYRKHLHGVVEKLFRCLSLG 235
           KEIDGK+GWVDHLFHIIWPPS+INY FWP+NP SYRAANEEYRKHLHGVVEKLFR LSLG
Sbjct: 121 KEIDGKRGWVDHLFHIIWPPSSINYNFWPQNPPSYRAANEEYRKHLHGVVEKLFRSLSLG 180

Query: 236 LGLEGHELKERAGGDKLVYLLKINYYPPCPRPDLALGVPAHTDMSAITILVPNEIQGLQA 295
           LGLEGHELKER GGD+LVYLLKINYYPPCPRPDLALGVPAHTDMSAITILVPNE+QGLQA
Sbjct: 181 LGLEGHELKERVGGDELVYLLKINYYPPCPRPDLALGVPAHTDMSAITILVPNEVQGLQA 240

Query: 296 FRDGKWYDVSYIPNALVIHIGDQIEILSNGKYKSVFHRTTVNKEKTRISWPVFLEPPPEF 355
           FRDG WYDV+YIPNALVIHIGDQIEILSNGKYKSV HRTTVNKEKTRISWPVFLEPPPEF
Sbjct: 241 FRDGHWYDVNYIPNALVIHIGDQIEILSNGKYKSVLHRTTVNKEKTRISWPVFLEPPPEF 300

Query: 356 EVGPHPKLIDEENPPKYKTKKYSEYAYCKLNKIPQ 391
           EVGPHPKL+DE+ P +YKTKKYSEYAYCKLNKIPQ
Sbjct: 301 EVGPHPKLVDEKIPARYKTKKYSEYAYCKLNKIPQ 335

[Close]

BLAST of ClCG08G005380 vs. ExPASy TrEMBL
Match: A0A6J1FDW3 (flavonol synthase/flavanone 3-hydroxylase-like OS=Cucurbita moschata OX=3662 GN=LOC111444648 PE=3 SV=1)

HSP 1 Score: 636.7 bits (1641), Expect = 6.2e-179
Identity = 293/335 (87.46%), Postives = 314/335 (93.73%), Query Frame = 0


Query: 56  MEVGRVQDIASSVFFSNTLPAEFIRPEAEQPGITTVKGHTLAVPVIDFTNPDDDSVLRAI 115
           MEVGRVQDIASS  FS +LPAEF+RP+ EQPGITTVKG  LAVPVIDF NPD+DSV++AI
Sbjct: 1   MEVGRVQDIASSALFSGSLPAEFVRPDTEQPGITTVKGQLLAVPVIDFKNPDEDSVIKAI 60

Query: 116 DDASRNWGMFQIVNHQIPNEAITHLQAVGKKFFELPPEEKEVYAKPPDSKSVEGYGTKLQ 175
           DDASRNWGMFQI+NH IPNEAI HLQAVGK FFELPPEEKEVYAKPPDSKSVEGYGTKLQ
Sbjct: 61  DDASRNWGMFQIINHDIPNEAIAHLQAVGKAFFELPPEEKEVYAKPPDSKSVEGYGTKLQ 120

Query: 176 KEIDGKKGWVDHLFHIIWPPSAINYIFWPKNPCSYRAANEEYRKHLHGVVEKLFRCLSLG 235
           KE+DG+KGWVDHLFHIIWPPSAIN+ FWP+NP SYRAANEEYRKHLHGVV+KLFR LSLG
Sbjct: 121 KEVDGRKGWVDHLFHIIWPPSAINFKFWPQNPPSYRAANEEYRKHLHGVVDKLFRSLSLG 180

Query: 236 LGLEGHELKERAGGDKLVYLLKINYYPPCPRPDLALGVPAHTDMSAITILVPNEIQGLQA 295
           LGLEGHELKE AGGDKLVYLLKINYYPPCP PDLALGVPAHTDMS ITILVPN++QGLQA
Sbjct: 181 LGLEGHELKESAGGDKLVYLLKINYYPPCPCPDLALGVPAHTDMSTITILVPNDVQGLQA 240

Query: 296 FRDGKWYDVSYIPNALVIHIGDQIEILSNGKYKSVFHRTTVNKEKTRISWPVFLEPPPEF 355
           FRDG WY+V+YIPNALVIHIGDQIEILSNGKYKSV HRTTV K++ RISWPVFLEPPPEF
Sbjct: 241 FRDGHWYNVNYIPNALVIHIGDQIEILSNGKYKSVLHRTTVKKDRVRISWPVFLEPPPEF 300

Query: 356 EVGPHPKLIDEENPPKYKTKKYSEYAYCKLNKIPQ 391
           EVGPHPKL+D++NPPKYKTKKY EYAYCKLNKIPQ
Sbjct: 301 EVGPHPKLVDQDNPPKYKTKKYGEYAYCKLNKIPQ 335

[Close]

BLAST of ClCG08G005380 vs. ExPASy TrEMBL
Match: A0A6J1JY72 (flavonol synthase/flavanone 3-hydroxylase-like OS=Cucurbita maxima OX=3661 GN=LOC111489355 PE=3 SV=1)

HSP 1 Score: 634.0 bits (1634), Expect = 4.0e-178
Identity = 291/335 (86.87%), Postives = 313/335 (93.43%), Query Frame = 0


Query: 56  MEVGRVQDIASSVFFSNTLPAEFIRPEAEQPGITTVKGHTLAVPVIDFTNPDDDSVLRAI 115
           MEVGRVQDIASS  FS ++PAEF+RP+ EQPGITTVKG  LAVPVIDF NPD+DSV++AI
Sbjct: 1   MEVGRVQDIASSALFSGSIPAEFVRPDPEQPGITTVKGQLLAVPVIDFKNPDEDSVIKAI 60

Query: 116 DDASRNWGMFQIVNHQIPNEAITHLQAVGKKFFELPPEEKEVYAKPPDSKSVEGYGTKLQ 175
           DDASRNWGMFQI+NH IPNEAI HLQAVGK FFELPPEEKEVYAKPPDSKSVEGYGTKLQ
Sbjct: 61  DDASRNWGMFQIINHDIPNEAIAHLQAVGKAFFELPPEEKEVYAKPPDSKSVEGYGTKLQ 120

Query: 176 KEIDGKKGWVDHLFHIIWPPSAINYIFWPKNPCSYRAANEEYRKHLHGVVEKLFRCLSLG 235
           KE+DGKKGWVDHLFHIIWPPSAIN+ FWP+NP SYR ANEEYRKHLHGVV+KLFR LSLG
Sbjct: 121 KEVDGKKGWVDHLFHIIWPPSAINFKFWPQNPPSYRVANEEYRKHLHGVVDKLFRSLSLG 180

Query: 236 LGLEGHELKERAGGDKLVYLLKINYYPPCPRPDLALGVPAHTDMSAITILVPNEIQGLQA 295
           LGLEGHELK+ AGGDKLVYLLKINYYPPCP PDLALGVPAHTDMS ITILVPN++QGLQA
Sbjct: 181 LGLEGHELKQSAGGDKLVYLLKINYYPPCPCPDLALGVPAHTDMSTITILVPNDVQGLQA 240

Query: 296 FRDGKWYDVSYIPNALVIHIGDQIEILSNGKYKSVFHRTTVNKEKTRISWPVFLEPPPEF 355
           FRDG WY+V+YIPNALVIHIGDQIEILSNGKYKSV HRTTV K++ RISWPVFLEPPPEF
Sbjct: 241 FRDGHWYNVNYIPNALVIHIGDQIEILSNGKYKSVLHRTTVKKDRARISWPVFLEPPPEF 300

Query: 356 EVGPHPKLIDEENPPKYKTKKYSEYAYCKLNKIPQ 391
           EVGPHPKL+D++NPPKYKTKKY EYAYCKLNKIPQ
Sbjct: 301 EVGPHPKLVDQDNPPKYKTKKYGEYAYCKLNKIPQ 335

[Close]

BLAST of ClCG08G005380 vs. TAIR 10
Match: AT5G08640.1 (flavonol synthase 1 )

HSP 1 Score: 457.2 bits (1175), Expect = 1.3e-128
Identity = 210/334 (62.87%), Postives = 255/334 (76.35%), Query Frame = 0


Query: 56  MEVGRVQDIASSVFFSNTLPAEFIRPEAEQPGITTVKGHTLAVPVIDFTNPDDDSVLRAI 115
           MEV RVQDI+SS   +  +P EFIR E EQP ITT +G T A+PV+D ++PD++SV RA+
Sbjct: 1   MEVERVQDISSSSLLTEAIPLEFIRSEKEQPAITTFRGPTPAIPVVDLSDPDEESVRRAV 60

Query: 116 DDASRNWGMFQIVNHQIPNEAITHLQAVGKKFFELPPEEKEVYAKPPDSKSVEGYGTKLQ 175
             AS  WG+FQ+VNH IP E I  LQ VG+KFFELP  EKE  AKP DSK +EGYGTKLQ
Sbjct: 61  VKASEEWGLFQVVNHGIPTELIRRLQDVGRKFFELPSSEKESVAKPEDSKDIEGYGTKLQ 120

Query: 176 KEIDGKKGWVDHLFHIIWPPSAINYIFWPKNPCSYRAANEEYRKHLHGVVEKLFRCLSLG 235
           K+ +GKK WVDHLFH IWPPS +NY FWPKNP  YR  NEEY  H+  + E L   LS G
Sbjct: 121 KDPEGKKAWVDHLFHRIWPPSCVNYRFWPKNPPEYREVNEEYAVHVKKLSETLLGILSDG 180

Query: 236 LGLEGHELKERAGGDKLVYLLKINYYPPCPRPDLALGVPAHTDMSAITILVPNEIQGLQA 295
           LGL+   LKE  GG+   Y++KINYYPPCPRPDLALGVPAHTD+S IT+LVPNE+ GLQ 
Sbjct: 181 LGLKRDALKEGLGGEMAEYMMKINYYPPCPRPDLALGVPAHTDLSGITLLVPNEVPGLQV 240

Query: 296 FRDGKWYDVSYIPNALVIHIGDQIEILSNGKYKSVFHRTTVNKEKTRISWPVFLEPPPEF 355
           F+D  W+D  YIP+A+++HIGDQI  LSNG+YK+V HRTTV+KEKTR+SWPVFLEPP E 
Sbjct: 241 FKDDHWFDAEYIPSAVIVHIGDQILRLSNGRYKNVLHRTTVDKEKTRMSWPVFLEPPREK 300

Query: 356 EVGPHPKLIDEENPPKYKTKKYSEYAYCKLNKIP 390
            VGP P+L  ++NPPK+K   + +Y+Y KLNK+P
Sbjct: 301 IVGPLPELTGDDNPPKFKPFAFKDYSYRKLNKLP 334

[Close]

BLAST of ClCG08G005380 vs. TAIR 10
Match: AT5G08640.2 (flavonol synthase 1 )

HSP 1 Score: 457.2 bits (1175), Expect = 1.3e-128
Identity = 210/334 (62.87%), Postives = 255/334 (76.35%), Query Frame = 0


Query: 56  MEVGRVQDIASSVFFSNTLPAEFIRPEAEQPGITTVKGHTLAVPVIDFTNPDDDSVLRAI 115
           MEV RVQDI+SS   +  +P EFIR E EQP ITT +G T A+PV+D ++PD++SV RA+
Sbjct: 1   MEVERVQDISSSSLLTEAIPLEFIRSEKEQPAITTFRGPTPAIPVVDLSDPDEESVRRAV 60

Query: 116 DDASRNWGMFQIVNHQIPNEAITHLQAVGKKFFELPPEEKEVYAKPPDSKSVEGYGTKLQ 175
             AS  WG+FQ+VNH IP E I  LQ VG+KFFELP  EKE  AKP DSK +EGYGTKLQ
Sbjct: 61  VKASEEWGLFQVVNHGIPTELIRRLQDVGRKFFELPSSEKESVAKPEDSKDIEGYGTKLQ 120

Query: 176 KEIDGKKGWVDHLFHIIWPPSAINYIFWPKNPCSYRAANEEYRKHLHGVVEKLFRCLSLG 235
           K+ +GKK WVDHLFH IWPPS +NY FWPKNP  YR  NEEY  H+  + E L   LS G
Sbjct: 121 KDPEGKKAWVDHLFHRIWPPSCVNYRFWPKNPPEYREVNEEYAVHVKKLSETLLGILSDG 180

Query: 236 LGLEGHELKERAGGDKLVYLLKINYYPPCPRPDLALGVPAHTDMSAITILVPNEIQGLQA 295
           LGL+   LKE  GG+   Y++KINYYPPCPRPDLALGVPAHTD+S IT+LVPNE+ GLQ 
Sbjct: 181 LGLKRDALKEGLGGEMAEYMMKINYYPPCPRPDLALGVPAHTDLSGITLLVPNEVPGLQV 240

Query: 296 FRDGKWYDVSYIPNALVIHIGDQIEILSNGKYKSVFHRTTVNKEKTRISWPVFLEPPPEF 355
           F+D  W+D  YIP+A+++HIGDQI  LSNG+YK+V HRTTV+KEKTR+SWPVFLEPP E 
Sbjct: 241 FKDDHWFDAEYIPSAVIVHIGDQILRLSNGRYKNVLHRTTVDKEKTRMSWPVFLEPPREK 300

Query: 356 EVGPHPKLIDEENPPKYKTKKYSEYAYCKLNKIP 390
            VGP P+L  ++NPPK+K   + +Y+Y KLNK+P
Sbjct: 301 IVGPLPELTGDDNPPKFKPFAFKDYSYRKLNKLP 334

[Close]

BLAST of ClCG08G005380 vs. TAIR 10
Match: AT5G63590.1 (flavonol synthase 3 )

HSP 1 Score: 343.6 bits (880), Expect = 2.1e-94
Identity = 159/295 (53.90%), Postives = 204/295 (69.15%), Query Frame = 0


Query: 95  TLAVPVIDFTNPDDDSVLRAIDDASRNWGMFQIVNHQIPNEAITHLQAVGKKFFELPPEE 154
           +L +PVID +NPD++ V  A+  AS+ WG+FQ+VNH IP E I  L  VG +FFELP  E
Sbjct: 11  SLDIPVIDLSNPDEELVASAVVKASQEWGIFQVVNHGIPTELILRLLQVGMEFFELPETE 70

Query: 155 KEVYAKPPDSKSVEGYGTKLQKEIDGKKGWVDHLFHIIWPPSAINYIFWPKNPCSYRAAN 214
           KE  AKP DS  +EGY TK QK+++G+  WVDHLFH IWPPS +N+ FWPKNP  Y   N
Sbjct: 71  KEAVAKPEDSLDIEGYRTKYQKDLEGRNAWVDHLFHRIWPPSRVNHKFWPKNPPEYIEVN 130

Query: 215 EEYRKHLHGVVEKLFRCLSLGLGLEGHELKERAGGDKLVYLLKINYYPPCPRPDLALGVP 274
           EEY  H+  + EK+   LS GLGL    LKE  GG+ + YL+KINYYPPCP P+L +G P
Sbjct: 131 EEYASHIKKLSEKIMEWLSEGLGLRHEALKEGLGGETIEYLMKINYYPPCPDPELVVGAP 190

Query: 275 AHTDMSAITILVPNEIQGLQAFRDGKWYDVSYIPNALVIHIGDQIEILSNGKYKSVFHRT 334
            HTD++ IT+LV NE  GLQAF+D +W D  Y  + +++ IGDQ   +SNGKYKSV HR 
Sbjct: 191 DHTDVNGITLLVANEALGLQAFKDNQWIDAEYTTSGIIVIIGDQFLRMSNGKYKSVEHRA 250

Query: 335 TVNKEKTRISWPVFLEPPPEFEVGPHPKLI-DEENPPKYKTKKYSEYAYCKLNKI 389
            ++KEKTRISWPVF+E   +   GP P+LI  +EN PK+K   Y +Y + KL K+
Sbjct: 251 KMDKEKTRISWPVFVESSLDQVFGPLPELITGDENVPKFKPYVYKDYKFRKLKKL 305

[Close]

BLAST of ClCG08G005380 vs. TAIR 10
Match: AT5G63600.1 (flavonol synthase 5 )

HSP 1 Score: 293.1 bits (749), Expect = 3.3e-79
Identity = 136/292 (46.58%), Postives = 190/292 (65.07%), Query Frame = 0


Query: 89  TTVKGHTLAVPVIDFTNPDDDSVLRAIDDASRNWGMFQIVNHQIPNEAITHLQAVGKKFF 148
           +T+ G  + VPV+D +  D+D ++R +  AS  WG+FQ+VNH IP E +  LQ VG +FF
Sbjct: 24  STLGGSAVDVPVVDLSVSDEDFLVREVVKASEEWGVFQVVNHGIPTELMRQLQMVGTQFF 83

Query: 149 ELPPEEKEVYAKPPDSKSVEGYGTKLQKEIDGKKGWVDHLFHIIWPPSAINYIFWPKNPC 208
           ELP  EKE  AK  D    EGY    +  + G   W +HLFH + PPS INY +WPKNP 
Sbjct: 84  ELPDAEKETVAKEED---FEGY---KKNYLGGINNWDEHLFHRLSPPSIINYKYWPKNPP 143

Query: 209 SYRAANEEYRKHLHGVVEKLFRCLSLGLGLEGHELKERAGGDKLVYLLKINYYPPCPRPD 268
            YR   EEY KH+  + EK+   LS GLGL+     +  GGD   Y+L++N+YPP    +
Sbjct: 144 QYREVTEEYTKHMKRLTEKILGWLSEGLGLQRETFTQSIGGDTAEYVLRVNFYPPTQDTE 203

Query: 269 LALGVPAHTDMSAITILVPNEIQGLQAFRDGKWYDVSYIPNALVIHIGDQIEILSNGKYK 328
           L +G  AH+DM AI +L+PNE+ GLQAF+D +W D+ YI +A+V+ IGDQ+  ++NG+ K
Sbjct: 204 LVIGAAAHSDMGAIALLIPNEVPGLQAFKDEQWLDLDYIDSAVVVIIGDQLMRMTNGRLK 263

Query: 329 SVFHRTTVNKEKTRISWPVFLEPPPEFEVGPHPKLIDEENPPKYKTKKYSEY 381
           +V HR   +K+K RISWPVF+ P  +  VGP P+   +ENPPK++T  Y++Y
Sbjct: 264 NVLHRAKSDKDKLRISWPVFVAPRADMSVGPLPEFTGDENPPKFETLIYNDY 309

[Close]

BLAST of ClCG08G005380 vs. TAIR 10
Match: AT5G63600.2 (flavonol synthase 5 )

HSP 1 Score: 290.8 bits (743), Expect = 1.6e-78
Identity = 136/293 (46.42%), Postives = 191/293 (65.19%), Query Frame = 0


Query: 89  TTVKGHTLAVPVIDFTNPDDDSVLRAIDDASRNWGMFQIVNHQIPNEAITHLQAVGKKFF 148
           +T+ G  + VPV+D +  D+D ++R +  AS  WG+FQ+VNH IP E +  LQ VG +FF
Sbjct: 24  STLGGSAVDVPVVDLSVSDEDFLVREVVKASEEWGVFQVVNHGIPTELMRQLQMVGTQFF 83

Query: 149 ELPPEEKEVYAKPPDSKSVEGYGTKLQKEIDGKKGWVDHLFHIIWPPSAINYIFWPKNPC 208
           ELP  EKE  AK  D    EGY    +  + G   W +HLFH + PPS INY +WPKNP 
Sbjct: 84  ELPDAEKETVAKEED---FEGY---KKNYLGGINNWDEHLFHRLSPPSIINYKYWPKNPP 143

Query: 209 SYRAANEEYRKHLHGVVEKLFRCLSLGLGLEGHELKERAGGDKLVYLLKINYYPPCPRPD 268
            YR   EEY KH+  + EK+   LS GLGL+     +  GGD   Y+L++N+YPP    +
Sbjct: 144 QYREVTEEYTKHMKRLTEKILGWLSEGLGLQRETFTQSIGGDTAEYVLRVNFYPPTQDTE 203

Query: 269 LALGVPAHTDMSAITILVPNEIQGLQAFRDGKWYDVSYIPNALVIHIGDQIEI-LSNGKY 328
           L +G  AH+DM AI +L+PNE+ GLQAF+D +W D+ YI +A+V+ IGDQ+ + ++NG+ 
Sbjct: 204 LVIGAAAHSDMGAIALLIPNEVPGLQAFKDEQWLDLDYIDSAVVVIIGDQLMVWMTNGRL 263

Query: 329 KSVFHRTTVNKEKTRISWPVFLEPPPEFEVGPHPKLIDEENPPKYKTKKYSEY 381
           K+V HR   +K+K RISWPVF+ P  +  VGP P+   +ENPPK++T  Y++Y
Sbjct: 264 KNVLHRAKSDKDKLRISWPVFVAPRADMSVGPLPEFTGDENPPKFETLIYNDY 310

The following BLAST results are available for this feature:

BLAST of ClCG08G005380 vs. NCBI nr


Match Name	E-value	Identity	Description
XP_038885635.1	7.1e-185	91.92	flavonol synthase/flavanone 3-hydroxylase [Benincasa hispida]	[more]
XP_008445671.1	3.6e-181	89.25	PREDICTED: flavonol synthase/flavanone 3-hydroxylase [Cucumis melo] >KAA0063921....	[more]
XP_023551148.1	2.6e-179	88.06	flavonol synthase/flavanone 3-hydroxylase-like [Cucurbita pepo subsp. pepo]	[more]
XP_004140862.1	4.4e-179	88.36	flavonol synthase/flavanone 3-hydroxylase [Cucumis sativus] >KGN45965.1 hypothet...	[more]
KAG7016505.1	5.8e-179	87.76	Flavonol synthase/flavanone 3-hydroxylase, partial [Cucurbita argyrosperma subsp...	[more]

BLAST of ClCG08G005380 vs. ExPASy Swiss-Prot


Match Name	E-value	Identity	Description
Q9XHG2	2.6e-145	74.22	Flavonol synthase/flavanone 3-hydroxylase OS=Malus domestica OX=3750 GN=FLS PE=2...	[more]
Q07512	3.3e-145	70.24	Flavonol synthase/flavanone 3-hydroxylase OS=Petunia hybrida OX=4102 GN=FL PE=2 ...	[more]
Q9M547	2.4e-143	70.45	Flavonol synthase/flavanone 3-hydroxylase OS=Eustoma exaltatum subsp. russellian...	[more]
Q9ZWQ9	1.0e-141	68.66	Flavonol synthase/flavanone 3-hydroxylase OS=Citrus unshiu OX=55188 GN=FLS PE=1 ...	[more]
Q7XZQ6	9.4e-140	68.84	Flavonol synthase/flavanone 3-hydroxylase OS=Petroselinum crispum OX=4043 GN=FLS...	[more]

BLAST of ClCG08G005380 vs. ExPASy TrEMBL


Match Name	E-value	Identity	Description
A0A5D3CHN5	1.8e-181	89.25	Flavonol synthase/flavanone 3-hydroxylase OS=Cucumis melo var. makuwa OX=1194695...	[more]
A0A1S3BCS7	1.8e-181	89.25	flavonol synthase/flavanone 3-hydroxylase OS=Cucumis melo OX=3656 GN=LOC10348863...	[more]
A0A0A0K885	2.1e-179	88.36	Fe2OG dioxygenase domain-containing protein OS=Cucumis sativus OX=3659 GN=Csa_6G...	[more]
A0A6J1FDW3	6.2e-179	87.46	flavonol synthase/flavanone 3-hydroxylase-like OS=Cucurbita moschata OX=3662 GN=...	[more]
A0A6J1JY72	4.0e-178	86.87	flavonol synthase/flavanone 3-hydroxylase-like OS=Cucurbita maxima OX=3661 GN=LO...	[more]

BLAST of ClCG08G005380 vs. TAIR 10


Match Name	E-value	Identity	Description
AT5G08640.1	1.3e-128	62.87	flavonol synthase 1	[more]
AT5G08640.2	1.3e-128	62.87	flavonol synthase 1	[more]
AT5G63590.1	2.1e-94	53.90	flavonol synthase 3	[more]
AT5G63600.1	3.3e-79	46.58	flavonol synthase 5	[more]
AT5G63600.2	1.6e-78	46.42	flavonol synthase 5	[more]

InterPro

Analysis Name: InterPro Annotations of Watermelon (Charleston Gray) v2.5
Date Performed: 2022-01-31


IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
IPR027443	Isopenicillin N synthase-like superfamily	GENE3D	2.60.120.330		coord: 56..388 e-value: 7.8E-95 score: 320.0
IPR044861	Isopenicillin N synthase-like, Fe(2+) 2OG dioxygenase domain	PFAM	PF03171	2OG-FeII_Oxy	coord: 256..351 e-value: 3.5E-25 score: 88.4
IPR026992	Non-haem dioxygenase N-terminal domain	PFAM	PF14226	DIOX_N	coord: 98..193 e-value: 1.2E-22 score: 80.8
None	No IPR available	PANTHER	PTHR47990:SF36	OS02G0767300 PROTEIN	coord: 60..387
None	No IPR available	PANTHER	PTHR47990	2-OXOGLUTARATE (2OG) AND FE(II)-DEPENDENT OXYGENASE SUPERFAMILY PROTEIN-RELATED	coord: 60..387
None	No IPR available	SUPERFAMILY	51197	Clavaminate synthase-like	coord: 71..388
IPR005123	Oxoglutarate/iron-dependent dioxygenase	PROSITE	PS51471	FE2OG_OXY	coord: 251..351 score: 14.764091

Relationships

The following mRNA feature(s) are a part of this gene:


Feature Name	Unique Name	Type
ClCG08G005380.2	ClCG08G005380.2	mRNA

GO Annotation

GO Assignments

This gene is annotated with the following GO terms.

Category	Term Accession	Term Name
biological_process	GO:0051555	flavonol biosynthetic process
biological_process	GO:0009416	response to light stimulus
molecular_function	GO:0045431	flavonol synthase activity
molecular_function	GO:0046872	metal ion binding