ClCG08G005240 (gene) Watermelon (Charleston Gray) v2.5

Overview
NameClCG08G005240
Typegene
OrganismCitrullus lanatus subsp. vulgaris cv. Charleston Gray (Watermelon (Charleston Gray) v2.5)
Descriptionprotein NRT1/ PTR FAMILY 3.1
LocationCG_Chr08: 16513254 .. 16518046 (-)
RNA-Seq ExpressionClCG08G005240
SyntenyClCG08G005240
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: exonfive_prime_UTRpolypeptideCDSthree_prime_UTR
Hold the cursor over a type above to highlight its positions in the sequence below.
AAACCACTGCCCAAAGAGCTTTGAGCAACAAAAAGTGGCCAAAAATGGAGGAGAAGAAGCAAACAAAGAGAAACAATGTAGAAAGGAAAAAGGGTGGCCTTATCACTATGCCATTCATCTTTGGTACAATTCTTGTTCTTTTCTTGTTCTTCTTCTTCAAGATCTGTTATTTTTAATTAAATTGATTAATAGTGGGATATTATATTGATTTGAAATTTTGGGTTTGATTATTATATGGTGAATTAGCTAATGAGATTTGCGAGAAGATTGCTGTGGTTGGTTTTAATACCAATATGATTAGCTACTTGACGAAGCAGCTTCATATGCCATTAACTAAAGGAGCTAACACCATCACTAATTTCAATGGAACCGCCAGCTTGACGCCGCTCATCGGAGCTTTCATCGCCGACTCTTACGTCGGCCGTTTCTGGACTATCACCGTCGCCTCTATCCTTTACCAAATTGTAAATCTTCTATTTAAACCTTCTAATTTTTCTTTCTACCCGTATCATTTTTTTTATTATTATTATTTATATATATATATATAATAGAGAAGAAAATTGGCCATACCTTTTTTTTTTTCTTCCTCCCTCCCCTTCCCTATCCTTTTAAATAGTGGAGAGAAAAAATAAATAAATAAAATAGAGGGGATTTGAACCAACCACAGTAAAAATGGGCCAATGAGTTCCAAAGTTTCTATTTTTTGTTTTTTTTTTTTTTTGGCAAATTGCAGAAACGTCCCTGAATTAGGAGGTTGTTGTAATTACACTTTCAAACTTTCAATTGTAAAAATTTGGCTCTCAAACTTACAACAATTTTAGAAGTTGGATCCTCAAGGATAAAAATTGAACCCTTAAACATATACAATTGTTATAAGTTCTATAATTCTACAAATTTGAGGGTTTAATTTTAACATTTGTGTAAGTTTGAGTGCTATAACAACTAAAAGTTTGAGGATGTAATTATAATTATTAATTTTAAGCATAGTTTTTGCAATTTGTCGTATATTATGTTTGTTTTAGCAAAATTTTTACTAAAAATGTACTGAGTACAAAGTAGAGGGAAAAATGTGTAATCTGATATATATATATATATATATATATATATATATATATATATATATATATATATNTGTGTAAGTTTGAGTGCTATAACAACTAAAAGTTTGAGGATGTAATTATAATTATTAATTTTAAGCATAGTTTTTGCAATTTGTCGTATATTATGTTTGTTTTAGCAAAATTTTTACTAAAAATGTACTGAGTACAAAGTAGAGGGAAAAATGTGTAATCTGATATATATATATATATATATATATATATATATATATATATATATTTTTTTTTAATTAAGACAATTTAAGAGCATAATAGACAGCCTTACAGCAATGGAAAAAATTAACTTAGATTGAGTAAAATTTTCAAGTTTATTGATAAATATGGAGTTTTCAAAATTAATTAACAATAATGAAGATTAAGAAGTCTAATCTTTTATTGAAGAATTTTTAATATAAAAGTAGTATATCTTTAAGTTCACCATTGTTCTTAAGATAGATTGTGTTTTCTTTTTTTAATTGAATAACCATTTATCTTGTAGTTTTAATATCATATTTCACTTCATGTTCATGTTCATGTTTATTTAATATGATATTAAAACCATCAAATTTATTTTTAAACAAATTGGGGATTAATTAACTTTAGTTGAAGTACGAGAAAAGATTCCCTTTTTAAATCCAAACTTTCTACCTCTAATAAAACATAATTCAATTAGTAAAATATTTAATCATTAAAATATCATAAGTTGGAATCTTCAGATGTTAAATGAAAACAATAATACATATAATATATGAGGACAAGATAAACTATCTTAGAAATGAAAAATATGGATTCAAGTACAATTTTGTCCCCATATTTTAAAGTTATTCCCTTTTAATTTTAATATTTTTAATAAATTTTAAATTTAGTTTATAATTAATTTTTTTTAAAAAAAATTATATTTTATGGTATTTTTATTACAAAATTTAAGAAGGAGTTGCAATTACACTTCAAACTTTCCACAGTAGAAATTGAACTCTTAAGCTTCTACAAATGCCAAAATTAAAACCTCAAGTTTACAATAATTGTAAAAATTGGTGAGACAAATACTAAAAATTGAATTCTTAAGATAAAATTACAAGTGTGATTAATAATTGTTTTGTTGTACGAAAATTATTGTTATTATTTAATGGGAAAATAGAAGTGGAGATTAATAATTGTGATGTTGGGTACAGGGGATGATAACCCTGACGACATCGGCAGTGGTGCCAGGGCTGAGGCCGCCACCGTGCAAGGGGGGAGAAATGTGCGAAGAAGCGAGGGGAGGAGAAGTAGGAATATTATACTTCTCTCTGCTGCTGACGGCGGTGGGGTCAGGCGGGATCCGGCCGTGCGTGGTGTCGTTCGGGGCGGACCAATTGGAGGAGGTGGATGGAAAGCAAGGGACGAAGACATACAAATACTTCAATTGGTATTATTTCGCGATGGGGGCATCGATGCTGGTGGCGGTGACGGTGCTTGTGTATGTGCAAGACAACATTGGGTGGGGGTGGGGATTTGGAATTCCCACCATTGCTATGTTCCTCTCCATTCTCACCTTCGTTGTCGGGTACCCGATTTACAGACACTTGGACCCGTCGGGTAGCCCCTTTACCCGACTCTTGCAAGTCGGGGTTGCGGCTTTTAGGAAGAGGAAACTCCAAATTCCCTCTAATTCCAATATGCTTTATCATAATCATCATATTGATGACCCAATTTCTCTTGATGGCAAGCTTCTCCATACCAAACATATGAGGTCTTTTTTCTTCTCTCCTTTTTACTTCAATTCATCTTTAACCACAAAACCAAATTAGTTTTCCAAGTTTCTTAACAAATCTCTAAAACAGAGAAAATTAAAGACTAAATTATTTGGTATATTATTAATGAATGACACATGTACTGTTTAATTTGAAAGAAAAAGATAGAATTGTTTTGTCATTCAGTGGTGAAGGCTCAACAAATGAGTATTGAGTCTCATCAACTTTGCCCTCTTTTTGACCAACAACACACTACACTTTTATTTTTATTTTTTTTTCCTTTGTCTTTTTTCCTAAACAACAAAACCACCTCTGTTTTCTAAATTCATTTCCAATGCTTATCTTTCCCAAATCCCCAAAATTTTATAAACAAATAAACTAGGTCCTCATTGTTTTTCATTTTTCCACAATTATCTCTTTCTCAAATTTTCTGCCCTTCACAAACATACATATTGTTTCTTAGTAATTTGGATTTCTAAATCTCTTTTTTCATATTAATAGTGTACTTTATTAGTGTTTTAGGAGGTGTTCAACCCCCAATGGGTGAATTGGGCTATTATAGCTCACTTCAAGTTTAGGGCTCTAATTATAATGGATGATAGTCTAATAGGACCTCTAAACTTTAAGAATGTGTAATAGATCCAAAATTAATCTATTTGATAGAAAATCAAAGTTCATGTTTAATAATAACCTAAACTCTGATATTTATGAGTATTTAAAAAAATTGAAAATTTGAGAATCTATTGAGCAGCTTTTAAAGTTTAAGAGACCGGATAGACACAAAACTAAAGGGGTTCATTAGCTTTATCATTAGACACTTCTTTAAAGGTAAGAGAGTTAAGTAAGTAAAGTTGTAGTGTAACTCAAAATAAATAAAAGAAAAATGCAAATGCAGTAATGTTTTAATAGATAGTAAATGCAGAGTCGAAAATGAACCTATATGGTTAATTGTAGTAACAAGTGAATGCAAATTTGAGGTATTTTTGCAACTCTGTAATATATCCATGAGTATAAAACTAACAACTACATTGTGATGACGACGCAGATTTCTAGACAAGGCAGCAATAGTGACAGAAGAAGAGAGAGTGAAAATGGGGGAAGAATTACAAAAACCAAATCTATGGAAACTAAGCACAGTCCATCGAGTAGAAGAGCTGAAATCTGTAATCCGAATGGGACCAATTTGGGCCTCAGGAATCATCCTTATCACAGCTTATTCCCAACAATACACATTTTCTCTCCTTCAAGGCTCAACAATGGACAGACATTTAACACACTCCTTCCAAATCCCAGCCGGTTCCATGACGGTCTTCACCCTTCTCACAATGCTCATCACCGTCGCCTTATACGACCGCATCTTCGTCCCCATCGCCCGCAAATTCACCGGCCTCGACCGCGGTATCACCTTCCTCAAGCGCATGGGCATCGGCCTCGTCATCTCCATCTTTGCCACATTCATCGCTGGCTTCGTCGAGCGAAAGCGCAAGCACGTCGCCTTCGCTCACGGCCTCCAAGATCACCCAGCTTCCACTATCCCCATCTCCGTCTTCTGGCTCGTCCCACAGTACAGCCTCCACGGCATGGCCGAGGCCTTCATGTCCATTGGCCATCTTGAATTTTTCTATGATCAATCCCCTGAGAGCATGAGGAGCACAGCCATGGCTCTGTTTTGGACTTCCATCTCGGCTGGTAACTATTTGAGTACTCTGTTGGTTACTTTGGTCCATAAGTACAGTGCTGGTCCGAATGGGTATAATTGGATTAGGAATGACAATATTAATAAGGGGAAGTTGGAAAATTTCTATTGGTTGGTTACTCTTTTGCAGGTTGTTAATCTTGTTTATTACATTGTTTGTGCAAAGCTTTATACTTTTAAGCCTTTGGAGGTTCAGAGAAAAGTTGTGGATAGCTCTAAGGTGGATGAAATTCAACTTGTAAACCCTGTTTAGTTTCCAAACATTTC

mRNA sequence

AAACCACTGCCCAAAGAGCTTTGAGCAACAAAAAGTGGCCAAAAATGGAGGAGAAGAAGCAAACAAAGAGAAACAATGTAGAAAGGAAAAAGGGTGGCCTTATCACTATGCCATTCATCTTTGCTAATGAGATTTGCGAGAAGATTGCTGTGGTTGGTTTTAATACCAATATGATTAGCTACTTGACGAAGCAGCTTCATATGCCATTAACTAAAGGAGCTAACACCATCACTAATTTCAATGGAACCGCCAGCTTGACGCCGCTCATCGGAGCTTTCATCGCCGACTCTTACGTCGGCCGTTTCTGGACTATCACCGTCGCCTCTATCCTTTACCAAATTGGGATGATAACCCTGACGACATCGGCAGTGGTGCCAGGGCTGAGGCCGCCACCGTGCAAGGGGGGAGAAATGTGCGAAGAAGCGAGGGGAGGAGAAGTAGGAATATTATACTTCTCTCTGCTGCTGACGGCGGTGGGGTCAGGCGGGATCCGGCCGTGCGTGGTGTCGTTCGGGGCGGACCAATTGGAGGAGGTGGATGGAAAGCAAGGGACGAAGACATACAAATACTTCAATTGGTATTATTTCGCGATGGGGGCATCGATGCTGGTGGCGGTGACGGTGCTTGTGTATGTGCAAGACAACATTGGGTGGGGGTGGGGATTTGGAATTCCCACCATTGCTATGTTCCTCTCCATTCTCACCTTCGTTGTCGGGTACCCGATTTACAGACACTTGGACCCGTCGGGTAGCCCCTTTACCCGACTCTTGCAAGTCGGGGTTGCGGCTTTTAGGAAGAGGAAACTCCAAATTCCCTCTAATTCCAATATGCTTTATCATAATCATCATATTGATGACCCAATTTCTCTTGATGGCAAGCTTCTCCATACCAAACATATGAGATTTCTAGACAAGGCAGCAATAGTGACAGAAGAAGAGAGAGTGAAAATGGGGGAAGAATTACAAAAACCAAATCTATGGAAACTAAGCACAGTCCATCGAGTAGAAGAGCTGAAATCTGTAATCCGAATGGGACCAATTTGGGCCTCAGGAATCATCCTTATCACAGCTTATTCCCAACAATACACATTTTCTCTCCTTCAAGGCTCAACAATGGACAGACATTTAACACACTCCTTCCAAATCCCAGCCGGTTCCATGACGGTCTTCACCCTTCTCACAATGCTCATCACCGTCGCCTTATACGACCGCATCTTCGTCCCCATCGCCCGCAAATTCACCGGCCTCGACCGCGGTATCACCTTCCTCAAGCGCATGGGCATCGGCCTCGTCATCTCCATCTTTGCCACATTCATCGCTGGCTTCGTCGAGCGAAAGCGCAAGCACGTCGCCTTCGCTCACGGCCTCCAAGATCACCCAGCTTCCACTATCCCCATCTCCGTCTTCTGGCTCGTCCCACAGTACAGCCTCCACGGCATGGCCGAGGCCTTCATGTCCATTGGCCATCTTGAATTTTTCTATGATCAATCCCCTGAGAGCATGAGGAGCACAGCCATGGCTCTGTTTTGGACTTCCATCTCGGCTGGTAACTATTTGAGTACTCTGTTGGTTACTTTGGTCCATAAGTACAGTGCTGGTCCGAATGGGTATAATTGGATTAGGAATGACAATATTAATAAGGGGAAGTTGGAAAATTTCTATTGGTTGGTTACTCTTTTGCAGGTTGTTAATCTTGTTTATTACATTGTTTGTGCAAAGCTTTATACTTTTAAGCCTTTGGAGGTTCAGAGAAAAGTTGTGGATAGCTCTAAGGTGGATGAAATTCAACTTGTAAACCCTGTTTAGTTTCCAAACATTTC

Coding sequence (CDS)

ATGGAGGAGAAGAAGCAAACAAAGAGAAACAATGTAGAAAGGAAAAAGGGTGGCCTTATCACTATGCCATTCATCTTTGCTAATGAGATTTGCGAGAAGATTGCTGTGGTTGGTTTTAATACCAATATGATTAGCTACTTGACGAAGCAGCTTCATATGCCATTAACTAAAGGAGCTAACACCATCACTAATTTCAATGGAACCGCCAGCTTGACGCCGCTCATCGGAGCTTTCATCGCCGACTCTTACGTCGGCCGTTTCTGGACTATCACCGTCGCCTCTATCCTTTACCAAATTGGGATGATAACCCTGACGACATCGGCAGTGGTGCCAGGGCTGAGGCCGCCACCGTGCAAGGGGGGAGAAATGTGCGAAGAAGCGAGGGGAGGAGAAGTAGGAATATTATACTTCTCTCTGCTGCTGACGGCGGTGGGGTCAGGCGGGATCCGGCCGTGCGTGGTGTCGTTCGGGGCGGACCAATTGGAGGAGGTGGATGGAAAGCAAGGGACGAAGACATACAAATACTTCAATTGGTATTATTTCGCGATGGGGGCATCGATGCTGGTGGCGGTGACGGTGCTTGTGTATGTGCAAGACAACATTGGGTGGGGGTGGGGATTTGGAATTCCCACCATTGCTATGTTCCTCTCCATTCTCACCTTCGTTGTCGGGTACCCGATTTACAGACACTTGGACCCGTCGGGTAGCCCCTTTACCCGACTCTTGCAAGTCGGGGTTGCGGCTTTTAGGAAGAGGAAACTCCAAATTCCCTCTAATTCCAATATGCTTTATCATAATCATCATATTGATGACCCAATTTCTCTTGATGGCAAGCTTCTCCATACCAAACATATGAGATTTCTAGACAAGGCAGCAATAGTGACAGAAGAAGAGAGAGTGAAAATGGGGGAAGAATTACAAAAACCAAATCTATGGAAACTAAGCACAGTCCATCGAGTAGAAGAGCTGAAATCTGTAATCCGAATGGGACCAATTTGGGCCTCAGGAATCATCCTTATCACAGCTTATTCCCAACAATACACATTTTCTCTCCTTCAAGGCTCAACAATGGACAGACATTTAACACACTCCTTCCAAATCCCAGCCGGTTCCATGACGGTCTTCACCCTTCTCACAATGCTCATCACCGTCGCCTTATACGACCGCATCTTCGTCCCCATCGCCCGCAAATTCACCGGCCTCGACCGCGGTATCACCTTCCTCAAGCGCATGGGCATCGGCCTCGTCATCTCCATCTTTGCCACATTCATCGCTGGCTTCGTCGAGCGAAAGCGCAAGCACGTCGCCTTCGCTCACGGCCTCCAAGATCACCCAGCTTCCACTATCCCCATCTCCGTCTTCTGGCTCGTCCCACAGTACAGCCTCCACGGCATGGCCGAGGCCTTCATGTCCATTGGCCATCTTGAATTTTTCTATGATCAATCCCCTGAGAGCATGAGGAGCACAGCCATGGCTCTGTTTTGGACTTCCATCTCGGCTGGTAACTATTTGAGTACTCTGTTGGTTACTTTGGTCCATAAGTACAGTGCTGGTCCGAATGGGTATAATTGGATTAGGAATGACAATATTAATAAGGGGAAGTTGGAAAATTTCTATTGGTTGGTTACTCTTTTGCAGGTTGTTAATCTTGTTTATTACATTGTTTGTGCAAAGCTTTATACTTTTAAGCCTTTGGAGGTTCAGAGAAAAGTTGTGGATAGCTCTAAGGTGGATGAAATTCAACTTGTAAACCCTGTTTAG

Protein sequence

MEEKKQTKRNNVERKKGGLITMPFIFANEICEKIAVVGFNTNMISYLTKQLHMPLTKGANTITNFNGTASLTPLIGAFIADSYVGRFWTITVASILYQIGMITLTTSAVVPGLRPPPCKGGEMCEEARGGEVGILYFSLLLTAVGSGGIRPCVVSFGADQLEEVDGKQGTKTYKYFNWYYFAMGASMLVAVTVLVYVQDNIGWGWGFGIPTIAMFLSILTFVVGYPIYRHLDPSGSPFTRLLQVGVAAFRKRKLQIPSNSNMLYHNHHIDDPISLDGKLLHTKHMRFLDKAAIVTEEERVKMGEELQKPNLWKLSTVHRVEELKSVIRMGPIWASGIILITAYSQQYTFSLLQGSTMDRHLTHSFQIPAGSMTVFTLLTMLITVALYDRIFVPIARKFTGLDRGITFLKRMGIGLVISIFATFIAGFVERKRKHVAFAHGLQDHPASTIPISVFWLVPQYSLHGMAEAFMSIGHLEFFYDQSPESMRSTAMALFWTSISAGNYLSTLLVTLVHKYSAGPNGYNWIRNDNINKGKLENFYWLVTLLQVVNLVYYIVCAKLYTFKPLEVQRKVVDSSKVDEIQLVNPV
Homology
BLAST of ClCG08G005240 vs. NCBI nr
Match: XP_038884610.1 (protein NRT1/ PTR FAMILY 3.1 [Benincasa hispida])

HSP 1 Score: 1107.8 bits (2864), Expect = 0.0e+00
Identity = 554/589 (94.06%), Postives = 571/589 (96.94%), Query Frame = 0

Query: 1   MEE--KKQTKRNNVERKKGGLITMPFIFANEICEKIAVVGFNTNMISYLTKQLHMPLTKG 60
           MEE  KKQT  N+VERKKGGLITMPFIFANEICEKIAVVGFNTNMISYLTKQLHMPLTKG
Sbjct: 1   MEEKVKKQTNTNHVERKKGGLITMPFIFANEICEKIAVVGFNTNMISYLTKQLHMPLTKG 60

Query: 61  ANTITNFNGTASLTPLIGAFIADSYVGRFWTITVASILYQIGMITLTTSAVVPGLRPPPC 120
           ANTITNFNGTASLTPLIGAFIADSYVGRFWTITVASILYQIGMITLTTSAVVPGLRPPPC
Sbjct: 61  ANTITNFNGTASLTPLIGAFIADSYVGRFWTITVASILYQIGMITLTTSAVVPGLRPPPC 120

Query: 121 KGGEMCEEARGGEVGILYFSLLLTAVGSGGIRPCVVSFGADQLEEVDGKQGTKTYKYFNW 180
           KGGE+CEEA GG++GILYFSLLLTA+GSGGIRPCVVSFGADQ +E D KQGTKTYKYFNW
Sbjct: 121 KGGEICEEASGGQLGILYFSLLLTALGSGGIRPCVVSFGADQFDETDPKQGTKTYKYFNW 180

Query: 181 YYFAMGASMLVAVTVLVYVQDNIGWGWGFGIPTIAMFLSILTFVVGYPIYRHLDPSGSPF 240
           YYFAMGASMLVAVTVLVYVQDNIGWGWGFGIPTIAMFLSILTFVVGYPIYRHLDPSGSPF
Sbjct: 181 YYFAMGASMLVAVTVLVYVQDNIGWGWGFGIPTIAMFLSILTFVVGYPIYRHLDPSGSPF 240

Query: 241 TRLLQVGVAAFRKRKLQIPSNSNMLYHNHHIDDPISLDGKLLHTKHMRFLDKAAIVTEEE 300
           TRLLQV VAAFRKRKL  P +SN+LYHNH IDDPIS DGKLLHTKHMRFLDKAAIVTEE+
Sbjct: 241 TRLLQVAVAAFRKRKLHSPYHSNLLYHNHDIDDPISRDGKLLHTKHMRFLDKAAIVTEED 300

Query: 301 RVKMG-EELQKPNLWKLSTVHRVEELKSVIRMGPIWASGIILITAYSQQYTFSLLQGSTM 360
           +VKMG E+L+KPNLWKL+TVHRVEELKSVIRMGPIWASGIILITAYSQQYTFSLLQGSTM
Sbjct: 301 KVKMGREDLEKPNLWKLNTVHRVEELKSVIRMGPIWASGIILITAYSQQYTFSLLQGSTM 360

Query: 361 DRHLTHSFQIPAGSMTVFTLLTMLITVALYDRIFVPIARKFTGLDRGITFLKRMGIGLVI 420
           DRHLT+SFQIPAGSMTVFTLLTMLITVALYDRIFVPIARKFTGLDRGITFLKRMGIGLVI
Sbjct: 361 DRHLTNSFQIPAGSMTVFTLLTMLITVALYDRIFVPIARKFTGLDRGITFLKRMGIGLVI 420

Query: 421 SIFATFIAGFVERKRKHVAFAHGLQDHPASTIPISVFWLVPQYSLHGMAEAFMSIGHLEF 480
           SIFATFIAGFVERKRKHVAF HGL+DHP STIPISVFWLVPQYSLHGMAEAFMSIGHLEF
Sbjct: 421 SIFATFIAGFVERKRKHVAFTHGLEDHPGSTIPISVFWLVPQYSLHGMAEAFMSIGHLEF 480

Query: 481 FYDQSPESMRSTAMALFWTSISAGNYLSTLLVTLVHKYSAGPNGYNWIRNDNINKGKLEN 540
           FYDQSPESMRSTAMALFWTSISAGNYLSTLLVTLVHKYSAGPNGYNWIRNDNINKGKLEN
Sbjct: 481 FYDQSPESMRSTAMALFWTSISAGNYLSTLLVTLVHKYSAGPNGYNWIRNDNINKGKLEN 540

Query: 541 FYWLVTLLQVVNLVYYIVCAKLYTFKPLEVQRKVVDSSKVDEIQLVNPV 587
           FYWLVTLLQV+NLVYYIVCAK YTFKPLEVQRKVVDSSKVDE+QLVNPV
Sbjct: 541 FYWLVTLLQVLNLVYYIVCAKFYTFKPLEVQRKVVDSSKVDELQLVNPV 589

BLAST of ClCG08G005240 vs. NCBI nr
Match: XP_022994014.1 (protein NRT1/ PTR FAMILY 3.1 [Cucurbita maxima])

HSP 1 Score: 1062.8 bits (2747), Expect = 1.1e-306
Identity = 524/584 (89.73%), Postives = 558/584 (95.55%), Query Frame = 0

Query: 3   EKKQTKRNNVERKKGGLITMPFIFANEICEKIAVVGFNTNMISYLTKQLHMPLTKGANTI 62
           E++Q+ RN+VERKKGGLITMPFIFANEICEKIAVVGFNTNMISYLTKQLHMPLTKGANTI
Sbjct: 2   EEQQSTRNHVERKKGGLITMPFIFANEICEKIAVVGFNTNMISYLTKQLHMPLTKGANTI 61

Query: 63  TNFNGTASLTPLIGAFIADSYVGRFWTITVASILYQIGMITLTTSAVVPGLRPPPCKGGE 122
           TNFNGTASLTPLIGAFIADSYVGRFWTITVASILYQIGMI+LTTSAVVPGLRPPPCKGGE
Sbjct: 62  TNFNGTASLTPLIGAFIADSYVGRFWTITVASILYQIGMISLTTSAVVPGLRPPPCKGGE 121

Query: 123 MCEEARGGEVGILYFSLLLTAVGSGGIRPCVVSFGADQLEEVDGKQGTKTYKYFNWYYFA 182
           +CEEA G ++GILYFSLLLTA+GSGGIRPCVVSFGADQ +E D KQG KTYKYFNWYYFA
Sbjct: 122 ICEEASGKQLGILYFSLLLTALGSGGIRPCVVSFGADQFDESDPKQGKKTYKYFNWYYFA 181

Query: 183 MGASMLVAVTVLVYVQDNIGWGWGFGIPTIAMFLSILTFVVGYPIYRHLDPSGSPFTRLL 242
           MGASMLVAVTVLVYVQDNIGWGWGFGIPTIAMFLSI+TFV+GY IYRHLDPSGSPFTRLL
Sbjct: 182 MGASMLVAVTVLVYVQDNIGWGWGFGIPTIAMFLSIITFVLGYSIYRHLDPSGSPFTRLL 241

Query: 243 QVGVAAFRKRKLQIPSNSNMLYHNHHIDDPISLDGKLLHTKHMRFLDKAAIVTEEERVKM 302
           QV VAAFRKRKL++  NS +LY NH IDDPIS DGKLLHT HMRFLDKAAIVTE+++VK+
Sbjct: 242 QVSVAAFRKRKLEMVPNSTLLYQNHDIDDPISRDGKLLHTNHMRFLDKAAIVTEQDKVKV 301

Query: 303 GEELQKPNLWKLSTVHRVEELKSVIRMGPIWASGIILITAYSQQYTFSLLQGSTMDRHLT 362
           GE   KPNLW L+TVHRVEELKSVIRMGPIWASGIILITAYSQQYTFSL QG+TMDR LT
Sbjct: 302 GE---KPNLWNLNTVHRVEELKSVIRMGPIWASGIILITAYSQQYTFSLQQGTTMDRRLT 361

Query: 363 HSFQIPAGSMTVFTLLTMLITVALYDRIFVPIARKFTGLDRGITFLKRMGIGLVISIFAT 422
            SF+IPAGSM+VFTLLTMLIT+ALYDRIF+PIAR+FTGLDRGITFLKRMGIGLVISIFAT
Sbjct: 362 DSFKIPAGSMSVFTLLTMLITIALYDRIFIPIARRFTGLDRGITFLKRMGIGLVISIFAT 421

Query: 423 FIAGFVERKRKHVAFAHGLQDHPASTIPISVFWLVPQYSLHGMAEAFMSIGHLEFFYDQS 482
            +AGFVERKRKHVAF HGLQDHP+STIPIS+FWLVPQYSLHGMAEAFMSIGHLEFFYDQS
Sbjct: 422 LVAGFVERKRKHVAFIHGLQDHPSSTIPISIFWLVPQYSLHGMAEAFMSIGHLEFFYDQS 481

Query: 483 PESMRSTAMALFWTSISAGNYLSTLLVTLVHKYSAGPNGYNWIRNDNINKGKLENFYWLV 542
           PESMRSTAMALFWTSISAGNYLSTLLVTLVHKY+AGPNGYNW+RNDNINKGKLENFYWLV
Sbjct: 482 PESMRSTAMALFWTSISAGNYLSTLLVTLVHKYTAGPNGYNWLRNDNINKGKLENFYWLV 541

Query: 543 TLLQVVNLVYYIVCAKLYTFKPLEVQRKVVDSSKVDEIQLVNPV 587
           TLLQV NLVYY+VCAK+YTFKPLEVQRKVVDSSKVDE+QLVNPV
Sbjct: 542 TLLQVFNLVYYVVCAKMYTFKPLEVQRKVVDSSKVDEVQLVNPV 582

BLAST of ClCG08G005240 vs. NCBI nr
Match: XP_023550855.1 (protein NRT1/ PTR FAMILY 3.1 [Cucurbita pepo subsp. pepo])

HSP 1 Score: 1059.7 bits (2739), Expect = 9.3e-306
Identity = 522/584 (89.38%), Postives = 557/584 (95.38%), Query Frame = 0

Query: 3   EKKQTKRNNVERKKGGLITMPFIFANEICEKIAVVGFNTNMISYLTKQLHMPLTKGANTI 62
           E++Q+ RN+VERKKGGLITMPFIFANEICEKIAVVGFNTNMISYLTKQLHMPLTKGANTI
Sbjct: 2   EEQQSTRNHVERKKGGLITMPFIFANEICEKIAVVGFNTNMISYLTKQLHMPLTKGANTI 61

Query: 63  TNFNGTASLTPLIGAFIADSYVGRFWTITVASILYQIGMITLTTSAVVPGLRPPPCKGGE 122
           TNFNGTASLTPLIGAFIADSYVGRFWTITVASILYQIGMI+LTTSAVVPGL+PPPCKGGE
Sbjct: 62  TNFNGTASLTPLIGAFIADSYVGRFWTITVASILYQIGMISLTTSAVVPGLKPPPCKGGE 121

Query: 123 MCEEARGGEVGILYFSLLLTAVGSGGIRPCVVSFGADQLEEVDGKQGTKTYKYFNWYYFA 182
           +CEEA G ++GILYFSLLLTA+GSGGIRPCVVSFGADQ +E D KQG KTYKYFNWYYFA
Sbjct: 122 ICEEASGKQLGILYFSLLLTALGSGGIRPCVVSFGADQFDESDPKQGKKTYKYFNWYYFA 181

Query: 183 MGASMLVAVTVLVYVQDNIGWGWGFGIPTIAMFLSILTFVVGYPIYRHLDPSGSPFTRLL 242
           MGASMLVAVTVLVYVQDNIGWGWGFGIPTIAMFLSI+TFV+GY IYRHLDPSGSPFTRLL
Sbjct: 182 MGASMLVAVTVLVYVQDNIGWGWGFGIPTIAMFLSIITFVLGYSIYRHLDPSGSPFTRLL 241

Query: 243 QVGVAAFRKRKLQIPSNSNMLYHNHHIDDPISLDGKLLHTKHMRFLDKAAIVTEEERVKM 302
           QV VAAFRKRKL++  NS +LY NH IDDPIS DGKLLHT HMRFLDKAA+VTE+++VK+
Sbjct: 242 QVSVAAFRKRKLEMVPNSTLLYQNHDIDDPISRDGKLLHTNHMRFLDKAAVVTEQDKVKL 301

Query: 303 GEELQKPNLWKLSTVHRVEELKSVIRMGPIWASGIILITAYSQQYTFSLLQGSTMDRHLT 362
           GE   KPNLW L+TVHRVEELKSVIRMGPIWASGIILITAYSQQYTFSL QG+TMDR LT
Sbjct: 302 GE---KPNLWNLNTVHRVEELKSVIRMGPIWASGIILITAYSQQYTFSLQQGTTMDRRLT 361

Query: 363 HSFQIPAGSMTVFTLLTMLITVALYDRIFVPIARKFTGLDRGITFLKRMGIGLVISIFAT 422
            SF+IPAGSM+VFTLLTMLIT+ALYDRIF+PIAR+FTGLDRGITFLKRMGIGLVISIFAT
Sbjct: 362 DSFKIPAGSMSVFTLLTMLITIALYDRIFIPIARRFTGLDRGITFLKRMGIGLVISIFAT 421

Query: 423 FIAGFVERKRKHVAFAHGLQDHPASTIPISVFWLVPQYSLHGMAEAFMSIGHLEFFYDQS 482
            +AGFVERKRKHVA  HGLQDHP+STIPIS+FWLVPQYSLHGMAEAFMSIGHLEFFYDQS
Sbjct: 422 LVAGFVERKRKHVALIHGLQDHPSSTIPISIFWLVPQYSLHGMAEAFMSIGHLEFFYDQS 481

Query: 483 PESMRSTAMALFWTSISAGNYLSTLLVTLVHKYSAGPNGYNWIRNDNINKGKLENFYWLV 542
           PESMRSTAMALFWTSISAGNYLSTLLVTLVHKY+AGPNGYNW+RNDNINKGKLENFYWLV
Sbjct: 482 PESMRSTAMALFWTSISAGNYLSTLLVTLVHKYTAGPNGYNWLRNDNINKGKLENFYWLV 541

Query: 543 TLLQVVNLVYYIVCAKLYTFKPLEVQRKVVDSSKVDEIQLVNPV 587
           TLLQV NLVYYIVCAK+YTFKPLEVQRKVVDSSKVDE+QLVNPV
Sbjct: 542 TLLQVFNLVYYIVCAKMYTFKPLEVQRKVVDSSKVDEVQLVNPV 582

BLAST of ClCG08G005240 vs. NCBI nr
Match: XP_022939103.1 (protein NRT1/ PTR FAMILY 3.1 [Cucurbita moschata])

HSP 1 Score: 1057.7 bits (2734), Expect = 3.5e-305
Identity = 522/583 (89.54%), Postives = 555/583 (95.20%), Query Frame = 0

Query: 3   EKKQTKRNNVERKKGGLITMPFIFANEICEKIAVVGFNTNMISYLTKQLHMPLTKGANTI 62
           E+ Q+ RN+VERKKGGLITMPFIFANEICEKIAVVGFNTNMISYLTKQLHMPLTKGANTI
Sbjct: 2   EEHQSTRNHVERKKGGLITMPFIFANEICEKIAVVGFNTNMISYLTKQLHMPLTKGANTI 61

Query: 63  TNFNGTASLTPLIGAFIADSYVGRFWTITVASILYQIGMITLTTSAVVPGLRPPPCKGGE 122
           TNFNGTASLTPLIGAFIADSYVGRFWTITVASILYQIGMI+LTTSAVVPGL+PPPCKGGE
Sbjct: 62  TNFNGTASLTPLIGAFIADSYVGRFWTITVASILYQIGMISLTTSAVVPGLKPPPCKGGE 121

Query: 123 MCEEARGGEVGILYFSLLLTAVGSGGIRPCVVSFGADQLEEVDGKQGTKTYKYFNWYYFA 182
           +CEEA G ++GILYFSLLLTA+GSGGIRPCVVSFGADQ +E D KQG KTYKYFNWYYFA
Sbjct: 122 ICEEASGKQLGILYFSLLLTALGSGGIRPCVVSFGADQFDESDPKQGKKTYKYFNWYYFA 181

Query: 183 MGASMLVAVTVLVYVQDNIGWGWGFGIPTIAMFLSILTFVVGYPIYRHLDPSGSPFTRLL 242
           MGASMLVAVTVLVYVQDNIGWGWGFGIPTIAMFLSI+TFV+GY IYRHLDPSGSPFTRLL
Sbjct: 182 MGASMLVAVTVLVYVQDNIGWGWGFGIPTIAMFLSIITFVLGYSIYRHLDPSGSPFTRLL 241

Query: 243 QVGVAAFRKRKLQIPSNSNMLYHNHHIDDPISLDGKLLHTKHMRFLDKAAIVTEEERVKM 302
           QV VAAFRKRKL++  NS +LY NH IDDPIS DGKLLHT HMRFLDKAAIVTE+++VK+
Sbjct: 242 QVSVAAFRKRKLEMVPNSTLLYQNHDIDDPISRDGKLLHTNHMRFLDKAAIVTEQDKVKV 301

Query: 303 GEELQKPNLWKLSTVHRVEELKSVIRMGPIWASGIILITAYSQQYTFSLLQGSTMDRHLT 362
           GE   KPNLW L+TVHRVEELKSVIRMGPIWASGIILITAYSQQYTFSL QG+TMDR LT
Sbjct: 302 GE---KPNLWNLNTVHRVEELKSVIRMGPIWASGIILITAYSQQYTFSLQQGTTMDRRLT 361

Query: 363 HSFQIPAGSMTVFTLLTMLITVALYDRIFVPIARKFTGLDRGITFLKRMGIGLVISIFAT 422
            SF+IPAGSM+VFTLLTMLIT+ALYDRIF+PIAR+FTGLDRGITFLKRMGIGLVISIFAT
Sbjct: 362 DSFKIPAGSMSVFTLLTMLITIALYDRIFIPIARRFTGLDRGITFLKRMGIGLVISIFAT 421

Query: 423 FIAGFVERKRKHVAFAHGLQDHPASTIPISVFWLVPQYSLHGMAEAFMSIGHLEFFYDQS 482
            +AGFVERKRKHVA  HGLQDHP+STIPIS+FWLVPQYSLHGMAEAFMSIGHLEFFYDQS
Sbjct: 422 LVAGFVERKRKHVALIHGLQDHPSSTIPISIFWLVPQYSLHGMAEAFMSIGHLEFFYDQS 481

Query: 483 PESMRSTAMALFWTSISAGNYLSTLLVTLVHKYSAGPNGYNWIRNDNINKGKLENFYWLV 542
           PESMRSTAMALFWTSISAGNYLSTLLVTLVHKY+AGPNGYNW+RNDNINKGKLENFYWLV
Sbjct: 482 PESMRSTAMALFWTSISAGNYLSTLLVTLVHKYTAGPNGYNWLRNDNINKGKLENFYWLV 541

Query: 543 TLLQVVNLVYYIVCAKLYTFKPLEVQRKVVDSSKVDEIQLVNP 586
           TLLQV NLVYYIVCAK+YTFKPLEVQRKVVDSSKVDE+QLVNP
Sbjct: 542 TLLQVFNLVYYIVCAKMYTFKPLEVQRKVVDSSKVDEVQLVNP 581

BLAST of ClCG08G005240 vs. NCBI nr
Match: KAG6579198.1 (Protein NRT1/ PTR FAMILY 3.1, partial [Cucurbita argyrosperma subsp. sororia])

HSP 1 Score: 1056.6 bits (2731), Expect = 7.8e-305
Identity = 522/584 (89.38%), Postives = 556/584 (95.21%), Query Frame = 0

Query: 3   EKKQTKRNNVERKKGGLITMPFIFANEICEKIAVVGFNTNMISYLTKQLHMPLTKGANTI 62
           E++Q+ RN+VERKKGGLITMPFIFANEICEKIAVVGFNTNMISYLTKQLHMPLTKGANTI
Sbjct: 40  EEQQSTRNHVERKKGGLITMPFIFANEICEKIAVVGFNTNMISYLTKQLHMPLTKGANTI 99

Query: 63  TNFNGTASLTPLIGAFIADSYVGRFWTITVASILYQIGMITLTTSAVVPGLRPPPCKGGE 122
           TNFNGTASLTPLIGAFIADSYVGRFWTITVASILYQIGMI+LTTSAVVPGL+PPPCKGGE
Sbjct: 100 TNFNGTASLTPLIGAFIADSYVGRFWTITVASILYQIGMISLTTSAVVPGLKPPPCKGGE 159

Query: 123 MCEEARGGEVGILYFSLLLTAVGSGGIRPCVVSFGADQLEEVDGKQGTKTYKYFNWYYFA 182
           +CEEA G ++GILYFSLLLTA+GSGGIRPCVVSFGADQ +E D KQG KTYKYFNWYYFA
Sbjct: 160 ICEEASGKQLGILYFSLLLTALGSGGIRPCVVSFGADQFDESDPKQGKKTYKYFNWYYFA 219

Query: 183 MGASMLVAVTVLVYVQDNIGWGWGFGIPTIAMFLSILTFVVGYPIYRHLDPSGSPFTRLL 242
           MGASMLVAVTVLVYVQDNIGWGWGFGIPTIAMFLSI+TFV+GY IYRHLDPSGSPFTRLL
Sbjct: 220 MGASMLVAVTVLVYVQDNIGWGWGFGIPTIAMFLSIITFVLGYSIYRHLDPSGSPFTRLL 279

Query: 243 QVGVAAFRKRKLQIPSNSNMLYHNHHIDDPISLDGKLLHTKHMRFLDKAAIVTEEERVKM 302
           QV VAAFRKRKL++  NS +LY NH IDDPIS DGKLLHT HMRFLDKAAIVTE+++VK+
Sbjct: 280 QVSVAAFRKRKLEMVPNSTLLYQNHDIDDPISRDGKLLHTNHMRFLDKAAIVTEQDKVKV 339

Query: 303 GEELQKPNLWKLSTVHRVEELKSVIRMGPIWASGIILITAYSQQYTFSLLQGSTMDRHLT 362
           GE   KP LW L+TVHRVEELKSVIRMGPIWASGIILITAYSQQYTFSL QG+TMDR LT
Sbjct: 340 GE---KPYLWNLNTVHRVEELKSVIRMGPIWASGIILITAYSQQYTFSLQQGTTMDRRLT 399

Query: 363 HSFQIPAGSMTVFTLLTMLITVALYDRIFVPIARKFTGLDRGITFLKRMGIGLVISIFAT 422
            SF+IPAGSM+VFTLLTMLIT+ALYDRIF+PIAR+FTGLDRGITFLKRMGIGLVISIFAT
Sbjct: 400 DSFKIPAGSMSVFTLLTMLITIALYDRIFIPIARRFTGLDRGITFLKRMGIGLVISIFAT 459

Query: 423 FIAGFVERKRKHVAFAHGLQDHPASTIPISVFWLVPQYSLHGMAEAFMSIGHLEFFYDQS 482
            +AGFVERKRKHVA  HGLQDHP+STIPIS+FWLVPQYSLHGMAEAFMSIGHLEFFYDQS
Sbjct: 460 LVAGFVERKRKHVALIHGLQDHPSSTIPISIFWLVPQYSLHGMAEAFMSIGHLEFFYDQS 519

Query: 483 PESMRSTAMALFWTSISAGNYLSTLLVTLVHKYSAGPNGYNWIRNDNINKGKLENFYWLV 542
           PESMRSTAMALFWTSISAGNYLSTLLVTLVHKY+AGPNGYNW+RNDNINKGKLENFYWLV
Sbjct: 520 PESMRSTAMALFWTSISAGNYLSTLLVTLVHKYTAGPNGYNWLRNDNINKGKLENFYWLV 579

Query: 543 TLLQVVNLVYYIVCAKLYTFKPLEVQRKVVDSSKVDEIQLVNPV 587
           TLLQV NLVYYIVCAK+YTFKPLEVQRKVVDSSKVDE+QLVNPV
Sbjct: 580 TLLQVFNLVYYIVCAKMYTFKPLEVQRKVVDSSKVDEVQLVNPV 620

BLAST of ClCG08G005240 vs. ExPASy Swiss-Prot
Match: Q9SX20 (Protein NRT1/ PTR FAMILY 3.1 OS=Arabidopsis thaliana OX=3702 GN=NPF3.1 PE=2 SV=1)

HSP 1 Score: 827.0 bits (2135), Expect = 1.3e-238
Identity = 410/584 (70.21%), Postives = 485/584 (83.05%), Query Frame = 0

Query: 2   EEKKQTKRNNVERKKGGLITMPFIFANEICEKIAVVGFNTNMISYLTKQLHMPLTKGANT 61
           EE+KQ       R KGGLITMPFIFANEICEK+AVVGF+ NMISYLT QLH+PLTK ANT
Sbjct: 11  EEEKQL-HGRPNRPKGGLITMPFIFANEICEKLAVVGFHANMISYLTTQLHLPLTKAANT 70

Query: 62  ITNFNGTASLTPLIGAFIADSYVGRFWTITVASILYQIGMITLTTSAVVPGLRPPPCKGG 121
           +TNF GT+SLTPL+GAFIADS+ GRFWTIT ASI+YQIGM  LT SA++P LRPPPCKG 
Sbjct: 71  LTNFAGTSSLTPLLGAFIADSFAGRFWTITFASIIYQIGMTLLTISAIIPTLRPPPCKGE 130

Query: 122 EMCEEARGGEVGILYFSLLLTAVGSGGIRPCVVSFGADQLEEVDGKQGTKTYKYFNWYYF 181
           E+C  A   ++ ILY +LLL A+GSGGIRPCVV+FGADQ +E D  Q TKT+ YFNWYYF
Sbjct: 131 EVCVVADTAQLSILYVALLLGALGSGGIRPCVVAFGADQFDESDPNQTTKTWNYFNWYYF 190

Query: 182 AMGASMLVAVTVLVYVQDNIGWGWGFGIPTIAMFLSILTFVVGYPIYRHLDPSGSPFTRL 241
            MGA++L+AVTVLV++QDN+GWG G GIPT+AMFLS++ FV G+ +YRHL P+GSPFTRL
Sbjct: 191 CMGAAVLLAVTVLVWIQDNVGWGLGLGIPTVAMFLSVIAFVGGFQLYRHLVPAGSPFTRL 250

Query: 242 LQVGVAAFRKRKLQIPSNSNMLYHNHHIDDPISLDGKLLHTKHMRFLDKAAIVTEEERVK 301
           +QVGVAAFRKRKL++ S+ ++LY N  ID PISL GKL HTKHM FLDKAAIVTEE+ +K
Sbjct: 251 IQVGVAAFRKRKLRMVSDPSLLYFNDEIDAPISLGGKLTHTKHMSFLDKAAIVTEEDNLK 310

Query: 302 MGEELQKPNLWKLSTVHRVEELKSVIRMGPIWASGIILITAYSQQYTFSLLQGSTMDRHL 361
            G   Q PN W+LSTVHRVEELKSVIRMGPI ASGI+LITAY+QQ TFSL Q  TM+RHL
Sbjct: 311 PG---QIPNHWRLSTVHRVEELKSVIRMGPIGASGILLITAYAQQGTFSLQQAKTMNRHL 370

Query: 362 THSFQIPAGSMTVFTLLTMLITVALYDRIFVPIARKFTGLDRGITFLKRMGIGLVISIFA 421
           T+SFQIPAGSM+VFT + ML T+  YDR+FV +ARKFTGL+RGITFL RMGIG VISI A
Sbjct: 371 TNSFQIPAGSMSVFTTVAMLTTIIFYDRVFVKVARKFTGLERGITFLHRMGIGFVISIIA 430

Query: 422 TFIAGFVERKRKHVAFAHGLQDHPASTIPISVFWLVPQYSLHGMAEAFMSIGHLEFFYDQ 481
           T +AGFVE KRK VA  HGL D P + +PIS  WL+PQY LHG+AEAFMSIGHLEFFYDQ
Sbjct: 431 TLVAGFVEVKRKSVAIEHGLLDKPHTIVPISFLWLIPQYGLHGVAEAFMSIGHLEFFYDQ 490

Query: 482 SPESMRSTAMALFWTSISAGNYLSTLLVTLVHKYSAGPNGYNWIRNDNINKGKLENFYWL 541
           +PESMRSTA ALFW +IS GNY+STLLVTLVHK+SA P+G NW+ ++N+N+G+LE FYWL
Sbjct: 491 APESMRSTATALFWMAISIGNYVSTLLVTLVHKFSAKPDGSNWLPDNNLNRGRLEYFYWL 550

Query: 542 VTLLQVVNLVYYIVCAKLYTFKPLEVQRKVVDSSKV-DEIQLVN 585
           +T+LQ VNLVYY+ CAK+YT+KP++V     DSS V +E+QL N
Sbjct: 551 ITVLQAVNLVYYLWCAKIYTYKPVQVHHSKEDSSPVKEELQLSN 590

BLAST of ClCG08G005240 vs. ExPASy Swiss-Prot
Match: Q9LFB8 (Protein NRT1/ PTR FAMILY 8.2 OS=Arabidopsis thaliana OX=3702 GN=NPF8.2 PE=2 SV=1)

HSP 1 Score: 490.0 bits (1260), Expect = 3.8e-137
Identity = 258/560 (46.07%), Postives = 362/560 (64.64%), Query Frame = 0

Query: 4   KKQTKRNNVERKKGGLITMPFIFANEICEKIAVVGFNTNMISYLTKQLHMPLTKGANTIT 63
           KK   +N    K G      FI   E CE++A  G +TN+I+YL KQ++M     + +++
Sbjct: 18  KKPANKN----KTGTWKACRFILGTECCERLAYYGMSTNLINYLEKQMNMENVSASKSVS 77

Query: 64  NFNGTASLTPLIGAFIADSYVGRFWTITVASILYQIGMITLTTSAVVPGLRPPPCKGGEM 123
           N++GT   TPLIGAFIAD+Y+GR+WTI    ++Y  GM  LT SA VPGL  P C  GE 
Sbjct: 78  NWSGTCYATPLIGAFIADAYLGRYWTIASFVVIYIAGMTLLTISASVPGL-TPTC-SGET 137

Query: 124 CEEARGGEVGILYFSLLLTAVGSGGIRPCVVSFGADQLEEVDGKQGTKTYKYFNWYYFAM 183
           C  A  G+  I + +L L A+G+GGI+PCV SFGADQ ++ D K+      +FNW+YF +
Sbjct: 138 C-HATAGQTAITFIALYLIALGTGGIKPCVSSFGADQFDDTDEKEKESKSSFFNWFYFVI 197

Query: 184 GASMLVAVTVLVYVQDNIGWGWGFGIPTIAMFLSILTFVVGYPIYRHLDPSGSPFTRLLQ 243
               ++A +VLV++Q N+GWGWG G+PT+AM ++++ F  G   YR   P GSP TR+LQ
Sbjct: 198 NVGAMIASSVLVWIQMNVGWGWGLGVPTVAMAIAVVFFFAGSNFYRLQKPGGSPLTRMLQ 257

Query: 244 VGVAAFRKRKLQIPSNSNMLYHNHHIDDPISLDGKLLHTKHMRFLDKAAIVTEEERVKMG 303
           V VA+ RK K++IP + ++LY N   +  I    KL HTK + F DKAA+ TE +     
Sbjct: 258 VIVASCRKSKVKIPEDESLLYENQDAESSIIGSRKLEHTKILTFFDKAAVETESD----N 317

Query: 304 EELQKPNLWKLSTVHRVEELKSVIRMGPIWASGIILITAYSQQYTFSLLQGSTMDRHLTH 363
           +   K + WKL TV +VEELK++IR+ PIWA+GI+  + YSQ  T  +LQG+T+D+H+  
Sbjct: 318 KGAAKSSSWKLCTVTQVEELKALIRLLPIWATGIVFASVYSQMGTVFVLQGNTLDQHMGP 377

Query: 364 SFQIPAGSMTVFTLLTMLITVALYDRIFVPIARKFTGLDRGITFLKRMGIGLVISIFATF 423
           +F+IP+ S+++F  L++L    +YD++ VP ARK+TG +RG T L+R+GIGLVISIF+  
Sbjct: 378 NFKIPSASLSLFDTLSVLFWAPVYDKLIVPFARKYTGHERGFTQLQRIGIGLVISIFSMV 437

Query: 424 IAGFVERKRKHVAFAHGLQDHPASTIPISVFWLVPQYSLHGMAEAFMSIGHLEFFYDQSP 483
            AG +E  R +    H L +    TIP+++FW VPQY L G AE F  IG LEFFYDQ+P
Sbjct: 438 SAGILEVARLNYVQTHNLYNE--ETIPMTIFWQVPQYFLVGCAEVFTFIGQLEFFYDQAP 497

Query: 484 ESMRSTAMALFWTSISAGNYLSTLLVTLVHKYSAGPNGYNWIRNDNINKGKLENFYWLVT 543
           ++MRS   AL  T+I+ GNYLST LVTLV K +       WI   N+N G L+ F+WL+ 
Sbjct: 498 DAMRSLCSALSLTAIAFGNYLSTFLVTLVTKVTRSGGRPGWIAK-NLNNGHLDYFFWLLA 557

Query: 544 LLQVVNLVYYIVCAKLYTFK 564
            L  +N + Y+  AK YT+K
Sbjct: 558 GLSFLNFLVYLWIAKWYTYK 563

BLAST of ClCG08G005240 vs. ExPASy Swiss-Prot
Match: Q9M390 (Protein NRT1/ PTR FAMILY 8.1 OS=Arabidopsis thaliana OX=3702 GN=NPF8.1 PE=1 SV=1)

HSP 1 Score: 482.6 bits (1241), Expect = 6.1e-135
Identity = 243/551 (44.10%), Postives = 354/551 (64.25%), Query Frame = 0

Query: 13  ERKKGGLITMPFIFANEICEKIAVVGFNTNMISYLTKQLHMPLTKGANTITNFNGTASLT 72
           + K G      FI  NE CE++A  G  TN+++YL  +L+      AN +TN++GT  +T
Sbjct: 22  KEKTGNWKACRFILGNECCERLAYYGMGTNLVNYLESRLNQGNATAANNVTNWSGTCYIT 81

Query: 73  PLIGAFIADSYVGRFWTITVASILYQIGMITLTTSAVVPGLRPPPCKGGEMCEEARGGEV 132
           PLIGAFIAD+Y+GR+WTI     +Y  GM  LT SA VPGL+P  C   + C      + 
Sbjct: 82  PLIGAFIADAYLGRYWTIATFVFIYVSGMTLLTLSASVPGLKPGNC-NADTC-HPNSSQT 141

Query: 133 GILYFSLLLTAVGSGGIRPCVVSFGADQLEEVDGKQGTKTYKYFNWYYFAMGASMLVAVT 192
            + + +L + A+G+GGI+PCV SFGADQ +E D  +  K   +FNW+YF++    L+A T
Sbjct: 142 AVFFVALYMIALGTGGIKPCVSSFGADQFDENDENEKIKKSSFFNWFYFSINVGALIAAT 201

Query: 193 VLVYVQDNIGWGWGFGIPTIAMFLSILTFVVGYPIYRHLDPSGSPFTRLLQVGVAAFRKR 252
           VLV++Q N+GWGWGFG+PT+AM +++  F  G   YR   P GSP TR+ QV VAAFRK 
Sbjct: 202 VLVWIQMNVGWGWGFGVPTVAMVIAVCFFFFGSRFYRLQRPGGSPLTRIFQVIVAAFRKI 261

Query: 253 KLQIPSNSNMLYHNHHIDDPISLDGKLLHTKHMRFLDKAAIVTEEERVKMGEELQKPNLW 312
            +++P + ++L+     +  I    KL+HT +++F DKAA+ ++ + +K GE     N W
Sbjct: 262 SVKVPEDKSLLFETADDESNIKGSRKLVHTDNLKFFDKAAVESQSDSIKDGE----VNPW 321

Query: 313 KLSTVHRVEELKSVIRMGPIWASGIILITAYSQQYTFSLLQGSTMDRHLTHSFQIPAGSM 372
           +L +V +VEELKS+I + P+WA+GI+  T YSQ  T  +LQG+TMD+H+  +F+IP+ S+
Sbjct: 322 RLCSVTQVEELKSIITLLPVWATGIVFATVYSQMSTMFVLQGNTMDQHMGKNFEIPSASL 381

Query: 373 TVFTLLTMLITVALYDRIFVPIARKFTGLDRGITFLKRMGIGLVISIFATFIAGFVERKR 432
           ++F  +++L    +YD+  +P+ARKFT  +RG T L+RMGIGLV+SIFA   AG +E  R
Sbjct: 382 SLFDTVSVLFWTPVYDQFIIPLARKFTRNERGFTQLQRMGIGLVVSIFAMITAGVLEVVR 441

Query: 433 KHVAFAHGLQDHPASTIPISVFWLVPQYSLHGMAEAFMSIGHLEFFYDQSPESMRSTAMA 492
                 H   D     I +S+FW +PQY L G AE F  IG LEFFYDQ+P++MRS   A
Sbjct: 442 LDYVKTHNAYDQ--KQIHMSIFWQIPQYLLIGCAEVFTFIGQLEFFYDQAPDAMRSLCSA 501

Query: 493 LFWTSISAGNYLSTLLVTLVHKYSAGPNGYNWIRNDNINKGKLENFYWLVTLLQVVNLVY 552
           L  T+++ GNYLST+LVT+V K +       WI  DN+N+G L+ F++L+  L  +N + 
Sbjct: 502 LSLTTVALGNYLSTVLVTVVMKITKKNGKPGWI-PDNLNRGHLDYFFYLLATLSFLNFLV 561

Query: 553 YIVCAKLYTFK 564
           Y+  +K Y +K
Sbjct: 562 YLWISKRYKYK 563

BLAST of ClCG08G005240 vs. ExPASy Swiss-Prot
Match: P46032 (Protein NRT1/ PTR FAMILY 8.3 OS=Arabidopsis thaliana OX=3702 GN=NPF8.3 PE=1 SV=1)

HSP 1 Score: 464.9 bits (1195), Expect = 1.3e-129
Identity = 248/552 (44.93%), Postives = 346/552 (62.68%), Query Frame = 0

Query: 12  VERKKGGLITMPFIFANEICEKIAVVGFNTNMISYLTKQLHMPLTKGANTITNFNGTASL 71
           ++ K G     PFI  NE CE++A  G   N+I+YLT +LH      A  +T + GT  L
Sbjct: 38  LKEKTGNWKACPFILGNECCERLAYYGIAGNLITYLTTKLHQGNVSAATNVTTWQGTCYL 97

Query: 72  TPLIGAFIADSYVGRFWTITVASILYQIGMITLTTSAVVPGLRPPPCKGGEMCEEARGGE 131
           TPLIGA +AD+Y GR+WTI   S +Y IGM  LT SA VP L+P  C  G+ C  A   +
Sbjct: 98  TPLIGAVLADAYWGRYWTIACFSGIYFIGMSALTLSASVPALKPAECI-GDFCPSATPAQ 157

Query: 132 VGILYFSLLLTAVGSGGIRPCVVSFGADQLEEVDGKQGTKTYKYFNWYYFAMGASMLVAV 191
             + +  L L A+G+GGI+PCV SFGADQ ++ D ++  +   +FNW+YF++    LV+ 
Sbjct: 158 YAMFFGGLYLIALGTGGIKPCVSSFGADQFDDTDSRERVRKASFFNWFYFSINIGALVSS 217

Query: 192 TVLVYVQDNIGWGWGFGIPTIAMFLSILTFVVGYPIYRHLDPSGSPFTRLLQVGVAAFRK 251
           ++LV++Q+N GWG GFGIPT+ M L+I +F  G P+YR   P GSP TR+ QV VA+FRK
Sbjct: 218 SLLVWIQENRGWGLGFGIPTVFMGLAIASFFFGTPLYRFQKPGGSPITRISQVVVASFRK 277

Query: 252 RKLQIPSNSNMLYHNHHIDDPISLDGKLLHTKHMRFLDKAAIVTEEERVKMGEELQKPNL 311
             +++P ++ +LY     +  I+   K+ HT   ++LDKAA+++EEE  K G+     N 
Sbjct: 278 SSVKVPEDATLLYETQDKNSAIAGSRKIEHTDDCQYLDKAAVISEEES-KSGD---YSNS 337

Query: 312 WKLSTVHRVEELKSVIRMGPIWASGIILITAYSQQYTFSLLQGSTMDRHLTHSFQIPAGS 371
           W+L TV +VEELK +IRM PIWASGII    Y+Q  T  + QG  M+  +  SFQ+P  +
Sbjct: 338 WRLCTVTQVEELKILIRMFPIWASGIIFSAVYAQMSTMFVQQGRAMNCKI-GSFQLPPAA 397

Query: 372 MTVFTLLTMLITVALYDRIFVPIARKFTGLDRGITFLKRMGIGLVISIFATFIAGFVERK 431
           +  F   +++I V LYDR  VP+ARKFTG+D+G T ++RMGIGL +S+     A  VE  
Sbjct: 398 LGTFDTASVIIWVPLYDRFIVPLARKFTGVDKGFTEIQRMGIGLFVSVLCMAAAAIVEII 457

Query: 432 RKHVAFAHGLQDHPASTIPISVFWLVPQYSLHGMAEAFMSIGHLEFFYDQSPESMRSTAM 491
           R H+A   GL +  A  +PISV W +PQY + G AE F  IG LEFFYDQSP++MRS   
Sbjct: 458 RLHMANDLGLVESGA-PVPISVLWQIPQYFILGAAEVFYFIGQLEFFYDQSPDAMRSLCS 517

Query: 492 ALFWTSISAGNYLSTLLVTLVHKYSAGPNGYNWIRNDNINKGKLENFYWLVTLLQVVNLV 551
           AL   + + GNYLS+L++TLV  ++       WI +DN+N G L+ F+WL+  L +VN+ 
Sbjct: 518 ALALLTNALGNYLSSLILTLVTYFTTRNGQEGWI-SDNLNSGHLDYFFWLLAGLSLVNMA 577

Query: 552 YYIVCAKLYTFK 564
            Y   A  Y  K
Sbjct: 578 VYFFSAARYKQK 581

BLAST of ClCG08G005240 vs. ExPASy Swiss-Prot
Match: Q93Z20 (Protein NRT1/ PTR FAMILY 8.5 OS=Arabidopsis thaliana OX=3702 GN=NPF8.5 PE=2 SV=1)

HSP 1 Score: 451.1 bits (1159), Expect = 2.0e-125
Identity = 242/549 (44.08%), Postives = 346/549 (63.02%), Query Frame = 0

Query: 13  ERKKGGLITMPFIFANEICEKIAVVGFNTNMISYLTKQLHMPLTKGANTITNFNGTASLT 72
           ++K G     PFI  NE CE++A  G   N+I+Y T +LH      A+ +  + GT  +T
Sbjct: 50  KKKTGNWKACPFILGNECCERLAYYGIAKNLITYYTSELHESNVSAASDVMIWQGTCYIT 109

Query: 73  PLIGAFIADSYVGRFWTITVASILYQIGMITLTTSAVVPGLRPPPCKG--GEMCEEARGG 132
           PLIGA IADSY GR+WTI   S +Y IGM  LT SA +P L+P  C G    +C  A   
Sbjct: 110 PLIGAVIADSYWGRYWTIASFSAIYFIGMALLTLSASLPVLKPAACAGVAAALCSPATTV 169

Query: 133 EVGILYFSLLLTAVGSGGIRPCVVSFGADQLEEVDGKQGTKTYKYFNWYYFAMGASMLVA 192
           +  + +  L L A+G+GGI+PCV SFGADQ ++ D ++  +   +FNW+YF++     ++
Sbjct: 170 QYAVFFTGLYLIALGTGGIKPCVSSFGADQFDDTDPRERVRKASFFNWFYFSINIGSFIS 229

Query: 193 VTVLVYVQDNIGWGWGFGIPTIAMFLSILTFVVGYPIYRHLDPSGSPFTRLLQVGVAAFR 252
            T+LV+VQ+N+GWG GF IPT+ M +SI +F +G P+YR   P GSP TR+ QV VAA+R
Sbjct: 230 STLLVWVQENVGWGLGFLIPTVFMGVSIASFFIGTPLYRFQKPGGSPITRVCQVLVAAYR 289

Query: 253 KRKLQIPSNSNMLYHNHHIDDPISLDGKLLHTKHMRFLDKAAIVTEEERVKMGEELQKPN 312
           K KL +P + + LY     +  I+   K+ HT   +FLDKAA+++E E  K G      N
Sbjct: 290 KLKLNLPEDISFLYETREKNSMIAGSRKIQHTDGYKFLDKAAVISEYES-KSG---AFSN 349

Query: 313 LWKLSTVHRVEELKSVIRMGPIWASGIILITAYSQQYTFSLLQGSTMDRHLTHSFQIPAG 372
            WKL TV +VEE+K++IRM PIWASGI+    YSQ  T  + QG +M+R +  SF+IP  
Sbjct: 350 PWKLCTVTQVEEVKTLIRMFPIWASGIVYSVLYSQISTLFVQQGRSMNR-IIRSFEIPPA 409

Query: 373 SMTVFTLLTMLITVALYDRIFVPIARKFTGLDRGITFLKRMGIGLVISIFATFIAGFVER 432
           S  VF  L +LI++ +YDR  VP  R+FTG+ +G+T L+RMGIGL +S+ +   A  VE 
Sbjct: 410 SFGVFDTLIVLISIPIYDRFLVPFVRRFTGIPKGLTDLQRMGIGLFLSVLSIAAAAIVET 469

Query: 433 KRKHVAFAHGLQDHPASTIPISVFWLVPQYSLHGMAEAFMSIGHLEFFYDQSPESMRSTA 492
            R  +A     QD     + +S+FW +PQY L G+AE F  IG +EFFYD+SP++MRS  
Sbjct: 470 VRLQLA-----QDF----VAMSIFWQIPQYILMGIAEVFFFIGRVEFFYDESPDAMRSVC 529

Query: 493 MALFWTSISAGNYLSTLLVTLVHKYSAGPNGYNWIRNDNINKGKLENFYWLVTLLQVVNL 552
            AL   + + G+YLS+L++TLV  ++A      W+  D++NKG L+ F+WL+  L +VN+
Sbjct: 530 SALALLNTAVGSYLSSLILTLVAYFTALGGKDGWV-PDDLNKGHLDYFFWLLVSLGLVNI 583

Query: 553 -VYYIVCAK 559
            VY ++C K
Sbjct: 590 PVYALICVK 583

BLAST of ClCG08G005240 vs. ExPASy TrEMBL
Match: A0A6J1K1R1 (protein NRT1/ PTR FAMILY 3.1 OS=Cucurbita maxima OX=3661 GN=LOC111489833 PE=3 SV=1)

HSP 1 Score: 1062.8 bits (2747), Expect = 5.3e-307
Identity = 524/584 (89.73%), Postives = 558/584 (95.55%), Query Frame = 0

Query: 3   EKKQTKRNNVERKKGGLITMPFIFANEICEKIAVVGFNTNMISYLTKQLHMPLTKGANTI 62
           E++Q+ RN+VERKKGGLITMPFIFANEICEKIAVVGFNTNMISYLTKQLHMPLTKGANTI
Sbjct: 2   EEQQSTRNHVERKKGGLITMPFIFANEICEKIAVVGFNTNMISYLTKQLHMPLTKGANTI 61

Query: 63  TNFNGTASLTPLIGAFIADSYVGRFWTITVASILYQIGMITLTTSAVVPGLRPPPCKGGE 122
           TNFNGTASLTPLIGAFIADSYVGRFWTITVASILYQIGMI+LTTSAVVPGLRPPPCKGGE
Sbjct: 62  TNFNGTASLTPLIGAFIADSYVGRFWTITVASILYQIGMISLTTSAVVPGLRPPPCKGGE 121

Query: 123 MCEEARGGEVGILYFSLLLTAVGSGGIRPCVVSFGADQLEEVDGKQGTKTYKYFNWYYFA 182
           +CEEA G ++GILYFSLLLTA+GSGGIRPCVVSFGADQ +E D KQG KTYKYFNWYYFA
Sbjct: 122 ICEEASGKQLGILYFSLLLTALGSGGIRPCVVSFGADQFDESDPKQGKKTYKYFNWYYFA 181

Query: 183 MGASMLVAVTVLVYVQDNIGWGWGFGIPTIAMFLSILTFVVGYPIYRHLDPSGSPFTRLL 242
           MGASMLVAVTVLVYVQDNIGWGWGFGIPTIAMFLSI+TFV+GY IYRHLDPSGSPFTRLL
Sbjct: 182 MGASMLVAVTVLVYVQDNIGWGWGFGIPTIAMFLSIITFVLGYSIYRHLDPSGSPFTRLL 241

Query: 243 QVGVAAFRKRKLQIPSNSNMLYHNHHIDDPISLDGKLLHTKHMRFLDKAAIVTEEERVKM 302
           QV VAAFRKRKL++  NS +LY NH IDDPIS DGKLLHT HMRFLDKAAIVTE+++VK+
Sbjct: 242 QVSVAAFRKRKLEMVPNSTLLYQNHDIDDPISRDGKLLHTNHMRFLDKAAIVTEQDKVKV 301

Query: 303 GEELQKPNLWKLSTVHRVEELKSVIRMGPIWASGIILITAYSQQYTFSLLQGSTMDRHLT 362
           GE   KPNLW L+TVHRVEELKSVIRMGPIWASGIILITAYSQQYTFSL QG+TMDR LT
Sbjct: 302 GE---KPNLWNLNTVHRVEELKSVIRMGPIWASGIILITAYSQQYTFSLQQGTTMDRRLT 361

Query: 363 HSFQIPAGSMTVFTLLTMLITVALYDRIFVPIARKFTGLDRGITFLKRMGIGLVISIFAT 422
            SF+IPAGSM+VFTLLTMLIT+ALYDRIF+PIAR+FTGLDRGITFLKRMGIGLVISIFAT
Sbjct: 362 DSFKIPAGSMSVFTLLTMLITIALYDRIFIPIARRFTGLDRGITFLKRMGIGLVISIFAT 421

Query: 423 FIAGFVERKRKHVAFAHGLQDHPASTIPISVFWLVPQYSLHGMAEAFMSIGHLEFFYDQS 482
            +AGFVERKRKHVAF HGLQDHP+STIPIS+FWLVPQYSLHGMAEAFMSIGHLEFFYDQS
Sbjct: 422 LVAGFVERKRKHVAFIHGLQDHPSSTIPISIFWLVPQYSLHGMAEAFMSIGHLEFFYDQS 481

Query: 483 PESMRSTAMALFWTSISAGNYLSTLLVTLVHKYSAGPNGYNWIRNDNINKGKLENFYWLV 542
           PESMRSTAMALFWTSISAGNYLSTLLVTLVHKY+AGPNGYNW+RNDNINKGKLENFYWLV
Sbjct: 482 PESMRSTAMALFWTSISAGNYLSTLLVTLVHKYTAGPNGYNWLRNDNINKGKLENFYWLV 541

Query: 543 TLLQVVNLVYYIVCAKLYTFKPLEVQRKVVDSSKVDEIQLVNPV 587
           TLLQV NLVYY+VCAK+YTFKPLEVQRKVVDSSKVDE+QLVNPV
Sbjct: 542 TLLQVFNLVYYVVCAKMYTFKPLEVQRKVVDSSKVDEVQLVNPV 582

BLAST of ClCG08G005240 vs. ExPASy TrEMBL
Match: A0A6J1FKQ8 (protein NRT1/ PTR FAMILY 3.1 OS=Cucurbita moschata OX=3662 GN=LOC111445106 PE=3 SV=1)

HSP 1 Score: 1057.7 bits (2734), Expect = 1.7e-305
Identity = 522/583 (89.54%), Postives = 555/583 (95.20%), Query Frame = 0

Query: 3   EKKQTKRNNVERKKGGLITMPFIFANEICEKIAVVGFNTNMISYLTKQLHMPLTKGANTI 62
           E+ Q+ RN+VERKKGGLITMPFIFANEICEKIAVVGFNTNMISYLTKQLHMPLTKGANTI
Sbjct: 2   EEHQSTRNHVERKKGGLITMPFIFANEICEKIAVVGFNTNMISYLTKQLHMPLTKGANTI 61

Query: 63  TNFNGTASLTPLIGAFIADSYVGRFWTITVASILYQIGMITLTTSAVVPGLRPPPCKGGE 122
           TNFNGTASLTPLIGAFIADSYVGRFWTITVASILYQIGMI+LTTSAVVPGL+PPPCKGGE
Sbjct: 62  TNFNGTASLTPLIGAFIADSYVGRFWTITVASILYQIGMISLTTSAVVPGLKPPPCKGGE 121

Query: 123 MCEEARGGEVGILYFSLLLTAVGSGGIRPCVVSFGADQLEEVDGKQGTKTYKYFNWYYFA 182
           +CEEA G ++GILYFSLLLTA+GSGGIRPCVVSFGADQ +E D KQG KTYKYFNWYYFA
Sbjct: 122 ICEEASGKQLGILYFSLLLTALGSGGIRPCVVSFGADQFDESDPKQGKKTYKYFNWYYFA 181

Query: 183 MGASMLVAVTVLVYVQDNIGWGWGFGIPTIAMFLSILTFVVGYPIYRHLDPSGSPFTRLL 242
           MGASMLVAVTVLVYVQDNIGWGWGFGIPTIAMFLSI+TFV+GY IYRHLDPSGSPFTRLL
Sbjct: 182 MGASMLVAVTVLVYVQDNIGWGWGFGIPTIAMFLSIITFVLGYSIYRHLDPSGSPFTRLL 241

Query: 243 QVGVAAFRKRKLQIPSNSNMLYHNHHIDDPISLDGKLLHTKHMRFLDKAAIVTEEERVKM 302
           QV VAAFRKRKL++  NS +LY NH IDDPIS DGKLLHT HMRFLDKAAIVTE+++VK+
Sbjct: 242 QVSVAAFRKRKLEMVPNSTLLYQNHDIDDPISRDGKLLHTNHMRFLDKAAIVTEQDKVKV 301

Query: 303 GEELQKPNLWKLSTVHRVEELKSVIRMGPIWASGIILITAYSQQYTFSLLQGSTMDRHLT 362
           GE   KPNLW L+TVHRVEELKSVIRMGPIWASGIILITAYSQQYTFSL QG+TMDR LT
Sbjct: 302 GE---KPNLWNLNTVHRVEELKSVIRMGPIWASGIILITAYSQQYTFSLQQGTTMDRRLT 361

Query: 363 HSFQIPAGSMTVFTLLTMLITVALYDRIFVPIARKFTGLDRGITFLKRMGIGLVISIFAT 422
            SF+IPAGSM+VFTLLTMLIT+ALYDRIF+PIAR+FTGLDRGITFLKRMGIGLVISIFAT
Sbjct: 362 DSFKIPAGSMSVFTLLTMLITIALYDRIFIPIARRFTGLDRGITFLKRMGIGLVISIFAT 421

Query: 423 FIAGFVERKRKHVAFAHGLQDHPASTIPISVFWLVPQYSLHGMAEAFMSIGHLEFFYDQS 482
            +AGFVERKRKHVA  HGLQDHP+STIPIS+FWLVPQYSLHGMAEAFMSIGHLEFFYDQS
Sbjct: 422 LVAGFVERKRKHVALIHGLQDHPSSTIPISIFWLVPQYSLHGMAEAFMSIGHLEFFYDQS 481

Query: 483 PESMRSTAMALFWTSISAGNYLSTLLVTLVHKYSAGPNGYNWIRNDNINKGKLENFYWLV 542
           PESMRSTAMALFWTSISAGNYLSTLLVTLVHKY+AGPNGYNW+RNDNINKGKLENFYWLV
Sbjct: 482 PESMRSTAMALFWTSISAGNYLSTLLVTLVHKYTAGPNGYNWLRNDNINKGKLENFYWLV 541

Query: 543 TLLQVVNLVYYIVCAKLYTFKPLEVQRKVVDSSKVDEIQLVNP 586
           TLLQV NLVYYIVCAK+YTFKPLEVQRKVVDSSKVDE+QLVNP
Sbjct: 542 TLLQVFNLVYYIVCAKMYTFKPLEVQRKVVDSSKVDEVQLVNP 581

BLAST of ClCG08G005240 vs. ExPASy TrEMBL
Match: A0A1S3BD88 (protein NRT1/ PTR FAMILY 3.1 OS=Cucumis melo OX=3656 GN=LOC103488612 PE=3 SV=1)

HSP 1 Score: 1027.7 bits (2656), Expect = 1.9e-296
Identity = 513/586 (87.54%), Postives = 547/586 (93.34%), Query Frame = 0

Query: 1   MEEKKQTKRNNVERKKGGLITMPFIFANEICEKIAVVGFNTNMISYLTKQLHMPLTKGAN 60
           ME +K  K  ++ERKKGGLITMPFIFANEICEKIAVVGFNTNMISYLTKQLHMP TK AN
Sbjct: 1   MEMEKTEK--HMERKKGGLITMPFIFANEICEKIAVVGFNTNMISYLTKQLHMPSTKAAN 60

Query: 61  TITNFNGTASLTPLIGAFIADSYVGRFWTITVASILYQIGMITLTTSAVVPGLRPPPCKG 120
           TITNF+GTASLTPLIGAFIADSYVGRFWTITVASILYQIGMI+LTTSAVVPGLRPPPCK 
Sbjct: 61  TITNFSGTASLTPLIGAFIADSYVGRFWTITVASILYQIGMISLTTSAVVPGLRPPPCKA 120

Query: 121 GEMCEEARGGEVGILYFSLLLTAVGSGGIRPCVVSFGADQLEEVDGKQGTKTYKYFNWYY 180
           GE+C+EA  G++GILY SLLLTA+GSGGIRPCVVSFGADQ EE D K+GTKTYKYFNWYY
Sbjct: 121 GEICKEATEGQLGILYVSLLLTALGSGGIRPCVVSFGADQFEEGDPKRGTKTYKYFNWYY 180

Query: 181 FAMGASMLVAVTVLVYVQDNIGWGWGFGIPTIAMFLSILTFVVGYPIYRHLDPSGSPFTR 240
           FAMGASMLVAVTVLVYVQDNIGWGWGFGIPTIAM +SI+TFVVGYPIYRHLDPSGSPF+R
Sbjct: 181 FAMGASMLVAVTVLVYVQDNIGWGWGFGIPTIAMLVSIITFVVGYPIYRHLDPSGSPFSR 240

Query: 241 LLQVGVAAFRKRKLQIPSNSNMLYHNHHIDDPISLDGKLLHTKHMRFLDKAAIVTEEERV 300
           LLQVGVAA+RKRK+   S+SN+LY N  ID PIS DG LLHTKHMRFLDKAA+VTEEE+V
Sbjct: 241 LLQVGVAAYRKRKVGSVSDSNLLYQNRDIDGPISRDGVLLHTKHMRFLDKAAVVTEEEKV 300

Query: 301 KMGEELQKPNLWKLSTVHRVEELKSVIRMGPIWASGIILITAYSQQYTFSLLQGSTMDRH 360
           KMGEEL KPNLWKLSTVHRVEELKSVIRMGPIWA+GIILITAYSQQ TFSLLQ STM+RH
Sbjct: 301 KMGEELGKPNLWKLSTVHRVEELKSVIRMGPIWAAGIILITAYSQQNTFSLLQASTMNRH 360

Query: 361 LTHSFQIPAGSMTVFTLLTMLITVALYDRIFVPIARKFTGLDRGITFLKRMGIGLVISIF 420
           LT+SFQIPAGSMTVFTLLTMLITVALYDR+F+PIARKFTGLDRGITFL RMGIGLVISIF
Sbjct: 361 LTNSFQIPAGSMTVFTLLTMLITVALYDRVFIPIARKFTGLDRGITFLTRMGIGLVISIF 420

Query: 421 ATFIAGFVERKRKHVAFAHGLQDHPASTIPISVFWLVPQYSLHGMAEAFMSIGHLEFFYD 480
           AT IAGFVE+KRKHVAF HGL DHP S IPISVFWLVPQYSLHGMAEAFMSIGHLEFFYD
Sbjct: 421 ATLIAGFVEQKRKHVAFNHGLMDHPTSIIPISVFWLVPQYSLHGMAEAFMSIGHLEFFYD 480

Query: 481 QSPESMRSTAMALFWTSISAGNYLSTLLVTLVHKYSAGPNGYNWIRNDNINKGKLENFYW 540
           QSPESMRSTAMALFWTSISAG+YLSTLLV+LVHKYS G NG NWIRNDNINKGKLENFYW
Sbjct: 481 QSPESMRSTAMALFWTSISAGSYLSTLLVSLVHKYSGGANGENWIRNDNINKGKLENFYW 540

Query: 541 LVTLLQVVNLVYYIVCAKLYTFKPLEVQRKVVDSSKVDEIQLVNPV 587
           L+T+LQV+NL YYIVCA+ YTFKPLEVQRKV+D SKVDE+QLVNPV
Sbjct: 541 LITVLQVLNLAYYIVCARFYTFKPLEVQRKVMDGSKVDELQLVNPV 584

BLAST of ClCG08G005240 vs. ExPASy TrEMBL
Match: A0A0A0K953 (Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_6G037500 PE=3 SV=1)

HSP 1 Score: 1013.8 bits (2620), Expect = 2.8e-292
Identity = 503/567 (88.71%), Postives = 533/567 (94.00%), Query Frame = 0

Query: 20  ITMPFIFANEICEKIAVVGFNTNMISYLTKQLHMPLTKGANTITNFNGTASLTPLIGAFI 79
           +T     +NEICEKIAVVGFNTNMISYLTKQLHMP TK ANTITNF+GTASLTPLIGAFI
Sbjct: 4   VTPSLSLSNEICEKIAVVGFNTNMISYLTKQLHMPSTKAANTITNFSGTASLTPLIGAFI 63

Query: 80  ADSYVGRFWTITVASILYQIGMITLTTSAVVPGLRPPPCKGGEMCEEARGGEVGILYFSL 139
           ADSYVGRFWTITVASILYQIGM++LTTSAVVPGLRPPPCK GE+C+EA GG++GILY SL
Sbjct: 64  ADSYVGRFWTITVASILYQIGMVSLTTSAVVPGLRPPPCKAGEICKEATGGQLGILYVSL 123

Query: 140 LLTAVGSGGIRPCVVSFGADQLEEVDGKQGTKTYKYFNWYYFAMGASMLVAVTVLVYVQD 199
           LLTA+GSGGIRPCVVSFGADQ EE D K+GTKTYKYFNWYYFAMGASMLVAVTVLVYVQD
Sbjct: 124 LLTALGSGGIRPCVVSFGADQFEEGDPKRGTKTYKYFNWYYFAMGASMLVAVTVLVYVQD 183

Query: 200 NIGWGWGFGIPTIAMFLSILTFVVGYPIYRHLDPSGSPFTRLLQVGVAAFRKRKLQIPSN 259
           NIGWGWGFGIPTIAM +SI+TFVVGYPIYRHLDPSGSPFTRLLQVGVAA++KRK+ I S+
Sbjct: 184 NIGWGWGFGIPTIAMLVSIITFVVGYPIYRHLDPSGSPFTRLLQVGVAAYKKRKVCIVSD 243

Query: 260 SNMLYHNHHIDDPISLDGKLLHTKHMRFLDKAAIVTEEERVKMGEELQKPNLWKLSTVHR 319
           SN+LY N  IDDPIS DG LLHTKHMRFLDKAAIVTEEE+VKMGEEL KPNLWKLSTVHR
Sbjct: 244 SNLLYQNRDIDDPISRDGNLLHTKHMRFLDKAAIVTEEEKVKMGEELGKPNLWKLSTVHR 303

Query: 320 VEELKSVIRMGPIWASGIILITAYSQQYTFSLLQGSTMDRHLTHSFQIPAGSMTVFTLLT 379
           VEELKSVIRMGPIWASGIILITAYSQQYTFSLLQ STM+RHLT+SFQIPAGSMTVFTLLT
Sbjct: 304 VEELKSVIRMGPIWASGIILITAYSQQYTFSLLQASTMNRHLTNSFQIPAGSMTVFTLLT 363

Query: 380 MLITVALYDRIFVPIARKFTGLDRGITFLKRMGIGLVISIFATFIAGFVERKRKHVAFAH 439
           MLITVALYDR+F+PIAR+FTGLDRGITFL RMGIGLVISIFAT IAGFVE+KRKHVAF H
Sbjct: 364 MLITVALYDRVFIPIARRFTGLDRGITFLTRMGIGLVISIFATLIAGFVEQKRKHVAFTH 423

Query: 440 GLQDHPASTIPISVFWLVPQYSLHGMAEAFMSIGHLEFFYDQSPESMRSTAMALFWTSIS 499
           GL DHP S IPISVFWLVPQYSLHGMAEAFMSIGHLEFFYDQSPESMRSTAMALFWTSIS
Sbjct: 424 GLIDHPTSIIPISVFWLVPQYSLHGMAEAFMSIGHLEFFYDQSPESMRSTAMALFWTSIS 483

Query: 500 AGNYLSTLLVTLVHKYSAGPNGYNWIRNDNINKGKLENFYWLVTLLQVVNLVYYIVCAKL 559
           AGNYLSTLLV+LVHKYS+G NG NWIRNDNI+KGKLENFYWLVT LQV+NL YYIVCAK 
Sbjct: 484 AGNYLSTLLVSLVHKYSSGANGENWIRNDNISKGKLENFYWLVTGLQVLNLAYYIVCAKF 543

Query: 560 YTFKPLEVQRKVVDSSKVDEIQLVNPV 587
           YTFKPLEVQRKVVDSSKVDE+QLVNPV
Sbjct: 544 YTFKPLEVQRKVVDSSKVDELQLVNPV 570

BLAST of ClCG08G005240 vs. ExPASy TrEMBL
Match: A0A6J1C163 (protein NRT1/ PTR FAMILY 3.1 OS=Momordica charantia OX=3673 GN=LOC111007508 PE=3 SV=1)

HSP 1 Score: 981.5 bits (2536), Expect = 1.6e-282
Identity = 491/584 (84.08%), Postives = 528/584 (90.41%), Query Frame = 0

Query: 3   EKKQTKRNNVERKKGGLITMPFIFANEICEKIAVVGFNTNMISYLTKQLHMPLTKGANTI 62
           E++QTK  +  RKKGGLITMPFIFANEICEKIAVVGFNTNMISYLTKQLHMPLT+ ANTI
Sbjct: 2   EEEQTKNKHAGRKKGGLITMPFIFANEICEKIAVVGFNTNMISYLTKQLHMPLTQAANTI 61

Query: 63  TNFNGTASLTPLIGAFIADSYVGRFWTITVASILYQIGMITLTTSAVVPGLRPPPCKGGE 122
           TNF+GTASLTPLIGAFIADSY GRFWTITVASILYQIGM  LTTSAVVP LRPPPC GGE
Sbjct: 62  TNFSGTASLTPLIGAFIADSYAGRFWTITVASILYQIGMTILTTSAVVPTLRPPPCNGGE 121

Query: 123 MCEEARGGEVGILYFSLLLTAVGSGGIRPCVVSFGADQLEEVDGKQGTKTYKYFNWYYFA 182
           +C +A GG++GILY SLLLTA+GSGGIRPCVVSFGADQ EE D K+G KTYKYFNWYYFA
Sbjct: 122 VCSQADGGQLGILYISLLLTALGSGGIRPCVVSFGADQFEESDPKRGMKTYKYFNWYYFA 181

Query: 183 MGASMLVAVTVLVYVQDNIGWGWGFGIPTIAMFLSILTFVVGYPIYRHLDPSGSPFTRLL 242
           MGASMLVAVTVLVYVQDNIGWGWGFGIPTIAMFLSI+TF+VGYPIYRHLDPSGSPFTRLL
Sbjct: 182 MGASMLVAVTVLVYVQDNIGWGWGFGIPTIAMFLSIVTFIVGYPIYRHLDPSGSPFTRLL 241

Query: 243 QVGVAAFRKRKLQIPSNSNMLYHNHHIDDPISLDGKLLHTKHMRFLDKAAIVTEEERVKM 302
           QVGVAA+RKRKL   S+  MLY NH IDDPIS DGKLLHTKHM+FLDKAAIVTEE+ +K 
Sbjct: 242 QVGVAAYRKRKLDPVSDYKMLYQNHEIDDPISRDGKLLHTKHMKFLDKAAIVTEEDELKT 301

Query: 303 GEELQKPNLWKLSTVHRVEELKSVIRMGPIWASGIILITAYSQQYTFSLLQGSTMDRHLT 362
           G   +KPNLWKL+TVHRVEELKSVIRMGPIWASGIILITAYSQQ TFSL Q STM+RH+T
Sbjct: 302 G---RKPNLWKLNTVHRVEELKSVIRMGPIWASGIILITAYSQQNTFSLQQASTMNRHVT 361

Query: 363 HSFQIPAGSMTVFTLLTMLITVALYDRIFVPIARKFTGLDRGITFLKRMGIGLVISIFAT 422
            SFQIPAGSMTVFTLLTML+TVALYDR+FVPIAR+FTGLDRGITFL+RMGIGLVIS+FAT
Sbjct: 362 ESFQIPAGSMTVFTLLTMLVTVALYDRVFVPIARRFTGLDRGITFLRRMGIGLVISVFAT 421

Query: 423 FIAGFVERKRKHVAFAHGLQDHPASTIPISVFWLVPQYSLHGMAEAFMSIGHLEFFYDQS 482
            IAGFVE+KRKH A             PISVFWLVPQYSLHGMAEAFMSIGHLEFFYDQS
Sbjct: 422 LIAGFVEKKRKHAAAGD----------PISVFWLVPQYSLHGMAEAFMSIGHLEFFYDQS 481

Query: 483 PESMRSTAMALFWTSISAGNYLSTLLVTLVHKYSAGPNGYNWIRNDNINKGKLENFYWLV 542
           PESMRSTAMALFWTSISAGNYLSTLLVTLVHK++AGP G NW+RNDN+N GKLENFYWL+
Sbjct: 482 PESMRSTAMALFWTSISAGNYLSTLLVTLVHKFTAGPGGSNWLRNDNLNLGKLENFYWLI 541

Query: 543 TLLQVVNLVYYIVCAKLYTFKPLEVQRKVVDSSKVDEIQLVNPV 587
           TLLQV NLVYY+ CAK+YTFKPLE+Q KVVDSSKVDE+QLVN V
Sbjct: 542 TLLQVFNLVYYVFCAKVYTFKPLELQGKVVDSSKVDEVQLVNHV 572

BLAST of ClCG08G005240 vs. TAIR 10
Match: AT1G68570.1 (Major facilitator superfamily protein )

HSP 1 Score: 827.0 bits (2135), Expect = 9.4e-240
Identity = 410/584 (70.21%), Postives = 485/584 (83.05%), Query Frame = 0

Query: 2   EEKKQTKRNNVERKKGGLITMPFIFANEICEKIAVVGFNTNMISYLTKQLHMPLTKGANT 61
           EE+KQ       R KGGLITMPFIFANEICEK+AVVGF+ NMISYLT QLH+PLTK ANT
Sbjct: 11  EEEKQL-HGRPNRPKGGLITMPFIFANEICEKLAVVGFHANMISYLTTQLHLPLTKAANT 70

Query: 62  ITNFNGTASLTPLIGAFIADSYVGRFWTITVASILYQIGMITLTTSAVVPGLRPPPCKGG 121
           +TNF GT+SLTPL+GAFIADS+ GRFWTIT ASI+YQIGM  LT SA++P LRPPPCKG 
Sbjct: 71  LTNFAGTSSLTPLLGAFIADSFAGRFWTITFASIIYQIGMTLLTISAIIPTLRPPPCKGE 130

Query: 122 EMCEEARGGEVGILYFSLLLTAVGSGGIRPCVVSFGADQLEEVDGKQGTKTYKYFNWYYF 181
           E+C  A   ++ ILY +LLL A+GSGGIRPCVV+FGADQ +E D  Q TKT+ YFNWYYF
Sbjct: 131 EVCVVADTAQLSILYVALLLGALGSGGIRPCVVAFGADQFDESDPNQTTKTWNYFNWYYF 190

Query: 182 AMGASMLVAVTVLVYVQDNIGWGWGFGIPTIAMFLSILTFVVGYPIYRHLDPSGSPFTRL 241
            MGA++L+AVTVLV++QDN+GWG G GIPT+AMFLS++ FV G+ +YRHL P+GSPFTRL
Sbjct: 191 CMGAAVLLAVTVLVWIQDNVGWGLGLGIPTVAMFLSVIAFVGGFQLYRHLVPAGSPFTRL 250

Query: 242 LQVGVAAFRKRKLQIPSNSNMLYHNHHIDDPISLDGKLLHTKHMRFLDKAAIVTEEERVK 301
           +QVGVAAFRKRKL++ S+ ++LY N  ID PISL GKL HTKHM FLDKAAIVTEE+ +K
Sbjct: 251 IQVGVAAFRKRKLRMVSDPSLLYFNDEIDAPISLGGKLTHTKHMSFLDKAAIVTEEDNLK 310

Query: 302 MGEELQKPNLWKLSTVHRVEELKSVIRMGPIWASGIILITAYSQQYTFSLLQGSTMDRHL 361
            G   Q PN W+LSTVHRVEELKSVIRMGPI ASGI+LITAY+QQ TFSL Q  TM+RHL
Sbjct: 311 PG---QIPNHWRLSTVHRVEELKSVIRMGPIGASGILLITAYAQQGTFSLQQAKTMNRHL 370

Query: 362 THSFQIPAGSMTVFTLLTMLITVALYDRIFVPIARKFTGLDRGITFLKRMGIGLVISIFA 421
           T+SFQIPAGSM+VFT + ML T+  YDR+FV +ARKFTGL+RGITFL RMGIG VISI A
Sbjct: 371 TNSFQIPAGSMSVFTTVAMLTTIIFYDRVFVKVARKFTGLERGITFLHRMGIGFVISIIA 430

Query: 422 TFIAGFVERKRKHVAFAHGLQDHPASTIPISVFWLVPQYSLHGMAEAFMSIGHLEFFYDQ 481
           T +AGFVE KRK VA  HGL D P + +PIS  WL+PQY LHG+AEAFMSIGHLEFFYDQ
Sbjct: 431 TLVAGFVEVKRKSVAIEHGLLDKPHTIVPISFLWLIPQYGLHGVAEAFMSIGHLEFFYDQ 490

Query: 482 SPESMRSTAMALFWTSISAGNYLSTLLVTLVHKYSAGPNGYNWIRNDNINKGKLENFYWL 541
           +PESMRSTA ALFW +IS GNY+STLLVTLVHK+SA P+G NW+ ++N+N+G+LE FYWL
Sbjct: 491 APESMRSTATALFWMAISIGNYVSTLLVTLVHKFSAKPDGSNWLPDNNLNRGRLEYFYWL 550

Query: 542 VTLLQVVNLVYYIVCAKLYTFKPLEVQRKVVDSSKV-DEIQLVN 585
           +T+LQ VNLVYY+ CAK+YT+KP++V     DSS V +E+QL N
Sbjct: 551 ITVLQAVNLVYYLWCAKIYTYKPVQVHHSKEDSSPVKEELQLSN 590

BLAST of ClCG08G005240 vs. TAIR 10
Match: AT5G01180.1 (peptide transporter 5 )

HSP 1 Score: 490.0 bits (1260), Expect = 2.7e-138
Identity = 258/560 (46.07%), Postives = 362/560 (64.64%), Query Frame = 0

Query: 4   KKQTKRNNVERKKGGLITMPFIFANEICEKIAVVGFNTNMISYLTKQLHMPLTKGANTIT 63
           KK   +N    K G      FI   E CE++A  G +TN+I+YL KQ++M     + +++
Sbjct: 18  KKPANKN----KTGTWKACRFILGTECCERLAYYGMSTNLINYLEKQMNMENVSASKSVS 77

Query: 64  NFNGTASLTPLIGAFIADSYVGRFWTITVASILYQIGMITLTTSAVVPGLRPPPCKGGEM 123
           N++GT   TPLIGAFIAD+Y+GR+WTI    ++Y  GM  LT SA VPGL  P C  GE 
Sbjct: 78  NWSGTCYATPLIGAFIADAYLGRYWTIASFVVIYIAGMTLLTISASVPGL-TPTC-SGET 137

Query: 124 CEEARGGEVGILYFSLLLTAVGSGGIRPCVVSFGADQLEEVDGKQGTKTYKYFNWYYFAM 183
           C  A  G+  I + +L L A+G+GGI+PCV SFGADQ ++ D K+      +FNW+YF +
Sbjct: 138 C-HATAGQTAITFIALYLIALGTGGIKPCVSSFGADQFDDTDEKEKESKSSFFNWFYFVI 197

Query: 184 GASMLVAVTVLVYVQDNIGWGWGFGIPTIAMFLSILTFVVGYPIYRHLDPSGSPFTRLLQ 243
               ++A +VLV++Q N+GWGWG G+PT+AM ++++ F  G   YR   P GSP TR+LQ
Sbjct: 198 NVGAMIASSVLVWIQMNVGWGWGLGVPTVAMAIAVVFFFAGSNFYRLQKPGGSPLTRMLQ 257

Query: 244 VGVAAFRKRKLQIPSNSNMLYHNHHIDDPISLDGKLLHTKHMRFLDKAAIVTEEERVKMG 303
           V VA+ RK K++IP + ++LY N   +  I    KL HTK + F DKAA+ TE +     
Sbjct: 258 VIVASCRKSKVKIPEDESLLYENQDAESSIIGSRKLEHTKILTFFDKAAVETESD----N 317

Query: 304 EELQKPNLWKLSTVHRVEELKSVIRMGPIWASGIILITAYSQQYTFSLLQGSTMDRHLTH 363
           +   K + WKL TV +VEELK++IR+ PIWA+GI+  + YSQ  T  +LQG+T+D+H+  
Sbjct: 318 KGAAKSSSWKLCTVTQVEELKALIRLLPIWATGIVFASVYSQMGTVFVLQGNTLDQHMGP 377

Query: 364 SFQIPAGSMTVFTLLTMLITVALYDRIFVPIARKFTGLDRGITFLKRMGIGLVISIFATF 423
           +F+IP+ S+++F  L++L    +YD++ VP ARK+TG +RG T L+R+GIGLVISIF+  
Sbjct: 378 NFKIPSASLSLFDTLSVLFWAPVYDKLIVPFARKYTGHERGFTQLQRIGIGLVISIFSMV 437

Query: 424 IAGFVERKRKHVAFAHGLQDHPASTIPISVFWLVPQYSLHGMAEAFMSIGHLEFFYDQSP 483
            AG +E  R +    H L +    TIP+++FW VPQY L G AE F  IG LEFFYDQ+P
Sbjct: 438 SAGILEVARLNYVQTHNLYNE--ETIPMTIFWQVPQYFLVGCAEVFTFIGQLEFFYDQAP 497

Query: 484 ESMRSTAMALFWTSISAGNYLSTLLVTLVHKYSAGPNGYNWIRNDNINKGKLENFYWLVT 543
           ++MRS   AL  T+I+ GNYLST LVTLV K +       WI   N+N G L+ F+WL+ 
Sbjct: 498 DAMRSLCSALSLTAIAFGNYLSTFLVTLVTKVTRSGGRPGWIAK-NLNNGHLDYFFWLLA 557

Query: 544 LLQVVNLVYYIVCAKLYTFK 564
            L  +N + Y+  AK YT+K
Sbjct: 558 GLSFLNFLVYLWIAKWYTYK 563

BLAST of ClCG08G005240 vs. TAIR 10
Match: AT3G54140.1 (peptide transporter 1 )

HSP 1 Score: 482.6 bits (1241), Expect = 4.4e-136
Identity = 243/551 (44.10%), Postives = 354/551 (64.25%), Query Frame = 0

Query: 13  ERKKGGLITMPFIFANEICEKIAVVGFNTNMISYLTKQLHMPLTKGANTITNFNGTASLT 72
           + K G      FI  NE CE++A  G  TN+++YL  +L+      AN +TN++GT  +T
Sbjct: 22  KEKTGNWKACRFILGNECCERLAYYGMGTNLVNYLESRLNQGNATAANNVTNWSGTCYIT 81

Query: 73  PLIGAFIADSYVGRFWTITVASILYQIGMITLTTSAVVPGLRPPPCKGGEMCEEARGGEV 132
           PLIGAFIAD+Y+GR+WTI     +Y  GM  LT SA VPGL+P  C   + C      + 
Sbjct: 82  PLIGAFIADAYLGRYWTIATFVFIYVSGMTLLTLSASVPGLKPGNC-NADTC-HPNSSQT 141

Query: 133 GILYFSLLLTAVGSGGIRPCVVSFGADQLEEVDGKQGTKTYKYFNWYYFAMGASMLVAVT 192
            + + +L + A+G+GGI+PCV SFGADQ +E D  +  K   +FNW+YF++    L+A T
Sbjct: 142 AVFFVALYMIALGTGGIKPCVSSFGADQFDENDENEKIKKSSFFNWFYFSINVGALIAAT 201

Query: 193 VLVYVQDNIGWGWGFGIPTIAMFLSILTFVVGYPIYRHLDPSGSPFTRLLQVGVAAFRKR 252
           VLV++Q N+GWGWGFG+PT+AM +++  F  G   YR   P GSP TR+ QV VAAFRK 
Sbjct: 202 VLVWIQMNVGWGWGFGVPTVAMVIAVCFFFFGSRFYRLQRPGGSPLTRIFQVIVAAFRKI 261

Query: 253 KLQIPSNSNMLYHNHHIDDPISLDGKLLHTKHMRFLDKAAIVTEEERVKMGEELQKPNLW 312
            +++P + ++L+     +  I    KL+HT +++F DKAA+ ++ + +K GE     N W
Sbjct: 262 SVKVPEDKSLLFETADDESNIKGSRKLVHTDNLKFFDKAAVESQSDSIKDGE----VNPW 321

Query: 313 KLSTVHRVEELKSVIRMGPIWASGIILITAYSQQYTFSLLQGSTMDRHLTHSFQIPAGSM 372
           +L +V +VEELKS+I + P+WA+GI+  T YSQ  T  +LQG+TMD+H+  +F+IP+ S+
Sbjct: 322 RLCSVTQVEELKSIITLLPVWATGIVFATVYSQMSTMFVLQGNTMDQHMGKNFEIPSASL 381

Query: 373 TVFTLLTMLITVALYDRIFVPIARKFTGLDRGITFLKRMGIGLVISIFATFIAGFVERKR 432
           ++F  +++L    +YD+  +P+ARKFT  +RG T L+RMGIGLV+SIFA   AG +E  R
Sbjct: 382 SLFDTVSVLFWTPVYDQFIIPLARKFTRNERGFTQLQRMGIGLVVSIFAMITAGVLEVVR 441

Query: 433 KHVAFAHGLQDHPASTIPISVFWLVPQYSLHGMAEAFMSIGHLEFFYDQSPESMRSTAMA 492
                 H   D     I +S+FW +PQY L G AE F  IG LEFFYDQ+P++MRS   A
Sbjct: 442 LDYVKTHNAYDQ--KQIHMSIFWQIPQYLLIGCAEVFTFIGQLEFFYDQAPDAMRSLCSA 501

Query: 493 LFWTSISAGNYLSTLLVTLVHKYSAGPNGYNWIRNDNINKGKLENFYWLVTLLQVVNLVY 552
           L  T+++ GNYLST+LVT+V K +       WI  DN+N+G L+ F++L+  L  +N + 
Sbjct: 502 LSLTTVALGNYLSTVLVTVVMKITKKNGKPGWI-PDNLNRGHLDYFFYLLATLSFLNFLV 561

Query: 553 YIVCAKLYTFK 564
           Y+  +K Y +K
Sbjct: 562 YLWISKRYKYK 563

BLAST of ClCG08G005240 vs. TAIR 10
Match: AT2G02040.1 (peptide transporter 2 )

HSP 1 Score: 464.9 bits (1195), Expect = 9.4e-131
Identity = 248/552 (44.93%), Postives = 346/552 (62.68%), Query Frame = 0

Query: 12  VERKKGGLITMPFIFANEICEKIAVVGFNTNMISYLTKQLHMPLTKGANTITNFNGTASL 71
           ++ K G     PFI  NE CE++A  G   N+I+YLT +LH      A  +T + GT  L
Sbjct: 38  LKEKTGNWKACPFILGNECCERLAYYGIAGNLITYLTTKLHQGNVSAATNVTTWQGTCYL 97

Query: 72  TPLIGAFIADSYVGRFWTITVASILYQIGMITLTTSAVVPGLRPPPCKGGEMCEEARGGE 131
           TPLIGA +AD+Y GR+WTI   S +Y IGM  LT SA VP L+P  C  G+ C  A   +
Sbjct: 98  TPLIGAVLADAYWGRYWTIACFSGIYFIGMSALTLSASVPALKPAECI-GDFCPSATPAQ 157

Query: 132 VGILYFSLLLTAVGSGGIRPCVVSFGADQLEEVDGKQGTKTYKYFNWYYFAMGASMLVAV 191
             + +  L L A+G+GGI+PCV SFGADQ ++ D ++  +   +FNW+YF++    LV+ 
Sbjct: 158 YAMFFGGLYLIALGTGGIKPCVSSFGADQFDDTDSRERVRKASFFNWFYFSINIGALVSS 217

Query: 192 TVLVYVQDNIGWGWGFGIPTIAMFLSILTFVVGYPIYRHLDPSGSPFTRLLQVGVAAFRK 251
           ++LV++Q+N GWG GFGIPT+ M L+I +F  G P+YR   P GSP TR+ QV VA+FRK
Sbjct: 218 SLLVWIQENRGWGLGFGIPTVFMGLAIASFFFGTPLYRFQKPGGSPITRISQVVVASFRK 277

Query: 252 RKLQIPSNSNMLYHNHHIDDPISLDGKLLHTKHMRFLDKAAIVTEEERVKMGEELQKPNL 311
             +++P ++ +LY     +  I+   K+ HT   ++LDKAA+++EEE  K G+     N 
Sbjct: 278 SSVKVPEDATLLYETQDKNSAIAGSRKIEHTDDCQYLDKAAVISEEES-KSGD---YSNS 337

Query: 312 WKLSTVHRVEELKSVIRMGPIWASGIILITAYSQQYTFSLLQGSTMDRHLTHSFQIPAGS 371
           W+L TV +VEELK +IRM PIWASGII    Y+Q  T  + QG  M+  +  SFQ+P  +
Sbjct: 338 WRLCTVTQVEELKILIRMFPIWASGIIFSAVYAQMSTMFVQQGRAMNCKI-GSFQLPPAA 397

Query: 372 MTVFTLLTMLITVALYDRIFVPIARKFTGLDRGITFLKRMGIGLVISIFATFIAGFVERK 431
           +  F   +++I V LYDR  VP+ARKFTG+D+G T ++RMGIGL +S+     A  VE  
Sbjct: 398 LGTFDTASVIIWVPLYDRFIVPLARKFTGVDKGFTEIQRMGIGLFVSVLCMAAAAIVEII 457

Query: 432 RKHVAFAHGLQDHPASTIPISVFWLVPQYSLHGMAEAFMSIGHLEFFYDQSPESMRSTAM 491
           R H+A   GL +  A  +PISV W +PQY + G AE F  IG LEFFYDQSP++MRS   
Sbjct: 458 RLHMANDLGLVESGA-PVPISVLWQIPQYFILGAAEVFYFIGQLEFFYDQSPDAMRSLCS 517

Query: 492 ALFWTSISAGNYLSTLLVTLVHKYSAGPNGYNWIRNDNINKGKLENFYWLVTLLQVVNLV 551
           AL   + + GNYLS+L++TLV  ++       WI +DN+N G L+ F+WL+  L +VN+ 
Sbjct: 518 ALALLTNALGNYLSSLILTLVTYFTTRNGQEGWI-SDNLNSGHLDYFFWLLAGLSLVNMA 577

Query: 552 YYIVCAKLYTFK 564
            Y   A  Y  K
Sbjct: 578 VYFFSAARYKQK 581

BLAST of ClCG08G005240 vs. TAIR 10
Match: AT1G62200.1 (Major facilitator superfamily protein )

HSP 1 Score: 451.1 bits (1159), Expect = 1.4e-126
Identity = 242/549 (44.08%), Postives = 346/549 (63.02%), Query Frame = 0

Query: 13  ERKKGGLITMPFIFANEICEKIAVVGFNTNMISYLTKQLHMPLTKGANTITNFNGTASLT 72
           ++K G     PFI  NE CE++A  G   N+I+Y T +LH      A+ +  + GT  +T
Sbjct: 50  KKKTGNWKACPFILGNECCERLAYYGIAKNLITYYTSELHESNVSAASDVMIWQGTCYIT 109

Query: 73  PLIGAFIADSYVGRFWTITVASILYQIGMITLTTSAVVPGLRPPPCKG--GEMCEEARGG 132
           PLIGA IADSY GR+WTI   S +Y IGM  LT SA +P L+P  C G    +C  A   
Sbjct: 110 PLIGAVIADSYWGRYWTIASFSAIYFIGMALLTLSASLPVLKPAACAGVAAALCSPATTV 169

Query: 133 EVGILYFSLLLTAVGSGGIRPCVVSFGADQLEEVDGKQGTKTYKYFNWYYFAMGASMLVA 192
           +  + +  L L A+G+GGI+PCV SFGADQ ++ D ++  +   +FNW+YF++     ++
Sbjct: 170 QYAVFFTGLYLIALGTGGIKPCVSSFGADQFDDTDPRERVRKASFFNWFYFSINIGSFIS 229

Query: 193 VTVLVYVQDNIGWGWGFGIPTIAMFLSILTFVVGYPIYRHLDPSGSPFTRLLQVGVAAFR 252
            T+LV+VQ+N+GWG GF IPT+ M +SI +F +G P+YR   P GSP TR+ QV VAA+R
Sbjct: 230 STLLVWVQENVGWGLGFLIPTVFMGVSIASFFIGTPLYRFQKPGGSPITRVCQVLVAAYR 289

Query: 253 KRKLQIPSNSNMLYHNHHIDDPISLDGKLLHTKHMRFLDKAAIVTEEERVKMGEELQKPN 312
           K KL +P + + LY     +  I+   K+ HT   +FLDKAA+++E E  K G      N
Sbjct: 290 KLKLNLPEDISFLYETREKNSMIAGSRKIQHTDGYKFLDKAAVISEYES-KSG---AFSN 349

Query: 313 LWKLSTVHRVEELKSVIRMGPIWASGIILITAYSQQYTFSLLQGSTMDRHLTHSFQIPAG 372
            WKL TV +VEE+K++IRM PIWASGI+    YSQ  T  + QG +M+R +  SF+IP  
Sbjct: 350 PWKLCTVTQVEEVKTLIRMFPIWASGIVYSVLYSQISTLFVQQGRSMNR-IIRSFEIPPA 409

Query: 373 SMTVFTLLTMLITVALYDRIFVPIARKFTGLDRGITFLKRMGIGLVISIFATFIAGFVER 432
           S  VF  L +LI++ +YDR  VP  R+FTG+ +G+T L+RMGIGL +S+ +   A  VE 
Sbjct: 410 SFGVFDTLIVLISIPIYDRFLVPFVRRFTGIPKGLTDLQRMGIGLFLSVLSIAAAAIVET 469

Query: 433 KRKHVAFAHGLQDHPASTIPISVFWLVPQYSLHGMAEAFMSIGHLEFFYDQSPESMRSTA 492
            R  +A     QD     + +S+FW +PQY L G+AE F  IG +EFFYD+SP++MRS  
Sbjct: 470 VRLQLA-----QDF----VAMSIFWQIPQYILMGIAEVFFFIGRVEFFYDESPDAMRSVC 529

Query: 493 MALFWTSISAGNYLSTLLVTLVHKYSAGPNGYNWIRNDNINKGKLENFYWLVTLLQVVNL 552
            AL   + + G+YLS+L++TLV  ++A      W+  D++NKG L+ F+WL+  L +VN+
Sbjct: 530 SALALLNTAVGSYLSSLILTLVAYFTALGGKDGWV-PDDLNKGHLDYFFWLLVSLGLVNI 583

Query: 553 -VYYIVCAK 559
            VY ++C K
Sbjct: 590 PVYALICVK 583

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
XP_038884610.10.0e+0094.06protein NRT1/ PTR FAMILY 3.1 [Benincasa hispida][more]
XP_022994014.11.1e-30689.73protein NRT1/ PTR FAMILY 3.1 [Cucurbita maxima][more]
XP_023550855.19.3e-30689.38protein NRT1/ PTR FAMILY 3.1 [Cucurbita pepo subsp. pepo][more]
XP_022939103.13.5e-30589.54protein NRT1/ PTR FAMILY 3.1 [Cucurbita moschata][more]
KAG6579198.17.8e-30589.38Protein NRT1/ PTR FAMILY 3.1, partial [Cucurbita argyrosperma subsp. sororia][more]
Match NameE-valueIdentityDescription
Q9SX201.3e-23870.21Protein NRT1/ PTR FAMILY 3.1 OS=Arabidopsis thaliana OX=3702 GN=NPF3.1 PE=2 SV=1[more]
Q9LFB83.8e-13746.07Protein NRT1/ PTR FAMILY 8.2 OS=Arabidopsis thaliana OX=3702 GN=NPF8.2 PE=2 SV=1[more]
Q9M3906.1e-13544.10Protein NRT1/ PTR FAMILY 8.1 OS=Arabidopsis thaliana OX=3702 GN=NPF8.1 PE=1 SV=1[more]
P460321.3e-12944.93Protein NRT1/ PTR FAMILY 8.3 OS=Arabidopsis thaliana OX=3702 GN=NPF8.3 PE=1 SV=1[more]
Q93Z202.0e-12544.08Protein NRT1/ PTR FAMILY 8.5 OS=Arabidopsis thaliana OX=3702 GN=NPF8.5 PE=2 SV=1[more]
Match NameE-valueIdentityDescription
A0A6J1K1R15.3e-30789.73protein NRT1/ PTR FAMILY 3.1 OS=Cucurbita maxima OX=3661 GN=LOC111489833 PE=3 SV... [more]
A0A6J1FKQ81.7e-30589.54protein NRT1/ PTR FAMILY 3.1 OS=Cucurbita moschata OX=3662 GN=LOC111445106 PE=3 ... [more]
A0A1S3BD881.9e-29687.54protein NRT1/ PTR FAMILY 3.1 OS=Cucumis melo OX=3656 GN=LOC103488612 PE=3 SV=1[more]
A0A0A0K9532.8e-29288.71Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_6G037500 PE=3 SV=1[more]
A0A6J1C1631.6e-28284.08protein NRT1/ PTR FAMILY 3.1 OS=Momordica charantia OX=3673 GN=LOC111007508 PE=3... [more]
Match NameE-valueIdentityDescription
AT1G68570.19.4e-24070.21Major facilitator superfamily protein [more]
AT5G01180.12.7e-13846.07peptide transporter 5 [more]
AT3G54140.14.4e-13644.10peptide transporter 1 [more]
AT2G02040.19.4e-13144.93peptide transporter 2 [more]
AT1G62200.11.4e-12644.08Major facilitator superfamily protein [more]
InterPro
Analysis Name: InterPro Annotations of Watermelon (Charleston Gray) v2.5
Date Performed: 2022-01-31
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR036259MFS transporter superfamilyGENE3D1.20.1250.20MFS general substrate transporter like domainscoord: 2..568
e-value: 2.8E-186
score: 621.8
IPR036259MFS transporter superfamilySUPERFAMILY103473MFS general substrate transportercoord: 20..558
IPR000109Proton-dependent oligopeptide transporter familyPFAMPF00854PTR2coord: 87..525
e-value: 9.1E-101
score: 337.5
IPR000109Proton-dependent oligopeptide transporter familyPANTHERPTHR11654OLIGOPEPTIDE TRANSPORTER-RELATEDcoord: 12..571
NoneNo IPR availablePANTHERPTHR11654:SF544BNAA07G24560D PROTEINcoord: 12..571
IPR018456PTR2 family proton/oligopeptide symporter, conserved sitePROSITEPS01022PTR2_1coord: 76..100

Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
ClCG08G005240.1ClCG08G005240.1mRNA


GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
biological_process GO:0010336 gibberellic acid homeostasis
biological_process GO:0006857 oligopeptide transport
biological_process GO:0055085 transmembrane transport
cellular_component GO:0016021 integral component of membrane
cellular_component GO:0097708 intracellular vesicle
cellular_component GO:0005886 plasma membrane
cellular_component GO:0016020 membrane
molecular_function GO:0022857 transmembrane transporter activity