ClCG08G005190 (gene) Watermelon (Charleston Gray) v2.5

Overview
NameClCG08G005190
Typegene
OrganismCitrullus lanatus subsp. vulgaris cv. Charleston Gray (Watermelon (Charleston Gray) v2.5)
DescriptionRegulator of Vps4 activity in the MVB pathway protein
LocationCG_Chr08: 16385989 .. 16387825 (-)
RNA-Seq ExpressionClCG08G005190
SyntenyClCG08G005190
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: exonfive_prime_UTRpolypeptideCDSthree_prime_UTR
Hold the cursor over a type above to highlight its positions in the sequence below.
CAAACCCTTCCAAGTTCCCACGCGCTTCATCTTCAACCCTTCTTCACACTGCCCTGTTCTCCACTCTGAAACAACCGTATATAAACAAATCAATTGAAATTCCACATAATCTGAATTAACCCATTTCGCCATGGGAAAAAAGCTTGACGCTTTTCTTGGTAGGAATTTCAGAGCCTCCAAATTCCGTCCCCTTCTCAGTCTTGCCGTCACTCGCCTTTCCATCCTCACTACCCAACGCCGTCTCAGATGCTCTCAGGCTCATTCCGACGTCCTTCAACTCCTCCAACTTCCCCACCACCACCGTGCTCTTCTTCGAGTCCGTTTCTCTTCTTCCCCCCCTTTCCTCGTCTTTTCATTTCCCATTCCGATTTATTGATTTCTGTTCTGTTTTTTCGTTCATAGGTCGAGAAAGTGATTGTGGATCAGAATGCTCTGGATGCCTATGTTTTGATCGAAGGGTATCTCAACCTCTTGCTTGAAAGAACCACCCTCCTCGAACAACAAAGGTTTGTTTCCTATTCTCTCTAATCTCCTTCTTCTACAGATGACTTGATTTGAGTTGTTGTTGTTGTTGAATTCAGTGAGTGCCCTGAGGAATTGAAAGAAGCGGTTGCAGGGTTGATATTTGCTGCTTCCAGATGTGGAGATTTCCCTGAACTTCATGAGATCAAATCAGTTTTGACCAATCGTTTTGGCAAGGAATTCACTGCTCGTGCCGTTGAATTACGCAACAATTGTGGAGTCAATCATTTGGTAATTTTGGATTCCGCTATTTTATATCTTTTTTCAGTCCTGATTTTTACTACATCCTCTGTTTCCTTCCTTTATTCATCTTGTGCTCTGTTTCTGTAGTTAATGCAAAAACTTTCCACAAGGCAGCCCAGTTTGGAGAGTAGAATGGAGGTCCTCAAAGCCATTGCTTCTGAGAATGGGATTGTATTGCAGATTGACCAACCTTACCCTTCCAATGAGGTATGCAACTTGACCTTTAATTTTGCTGCATACGGCTGTTAAGAATCAAACCCCAAAATCTTAAAACGATAGATAATGAGATATTTAAAATCAGTCTTCTTCAAACCCGATTTTGATTGGCAATTGGGATTCTGTTTCTGATCATCACAGGAAAAACTAGTCAGAAACACAAGGCAAAACCAGGCAGAGGCACACAGTGAAGTGAAAGAGAAGTTTCAATTTTTCACTGAAGTGCCATCTGGTTCTCAACAGAAGTACAAAGATGTGGCGGATGCAGCGCAAGCCGCTTTCGAATCCGCAGCTCAAGCAGCAGCTGCTGCCCGAGCTGCCATGGAGCTCTCCCGCTCCCATGATGGTCCAAGCAGCCCGAATAGCCCTGGCTCTGGAACAACTCCACATAACAAACAAGAGGTAGAGGAACCAAAGGTTGAATCAAAACCAAAACCAAAAATGGAATATGGAATTCGGAGAGAAGGAGAAGGAGAAGGAGAAGGAGAAGAAGAAGTGAAAAATTCAGTGCCTGGTGCTTCTTCTCCAAGTAGAGAGTTCTGTTATGGGGAAAAGGAAAAGAGTGACATGGAAACAAAGTTAGAGAAGACAGAGTTAAGTGAGAAGGCAAGCTTTCGTTTAAATCTGGAGAAGCAGCCAATTTCAGTGAGAACGAGAAGAGTGCGAGGATACTGAAAAATATATGTAGTTCAGAATTGGGTTTGCTTTTGTATTCCTTCTACATATGTATAATTTTATTTTTGGTTTGGAAAGATCATGAAAATTGTGTAATTATGTGTGTGCAAATATTGGAATTTTATTGTTAAATTCGTTACTTTACAATGAAAACAATACGTGGGTTAATATACAAAATGTA

mRNA sequence

CAAACCCTTCCAAGTTCCCACGCGCTTCATCTTCAACCCTTCTTCACACTGCCCTGTTCTCCACTCTGAAACAACCGTATATAAACAAATCAATTGAAATTCCACATAATCTGAATTAACCCATTTCGCCATGGGAAAAAAGCTTGACGCTTTTCTTGGTAGGAATTTCAGAGCCTCCAAATTCCGTCCCCTTCTCAGTCTTGCCGTCACTCGCCTTTCCATCCTCACTACCCAACGCCGTCTCAGATGCTCTCAGGCTCATTCCGACGTCCTTCAACTCCTCCAACTTCCCCACCACCACCGTGCTCTTCTTCGAGTCGAGAAAGTGATTGTGGATCAGAATGCTCTGGATGCCTATGTTTTGATCGAAGGGTATCTCAACCTCTTGCTTGAAAGAACCACCCTCCTCGAACAACAAAGTGAGTGCCCTGAGGAATTGAAAGAAGCGGTTGCAGGGTTGATATTTGCTGCTTCCAGATGTGGAGATTTCCCTGAACTTCATGAGATCAAATCAGTTTTGACCAATCGTTTTGGCAAGGAATTCACTGCTCGTGCCGTTGAATTACGCAACAATTGTGGAGTCAATCATTTGTTAATGCAAAAACTTTCCACAAGGCAGCCCAGTTTGGAGAGTAGAATGGAGGTCCTCAAAGCCATTGCTTCTGAGAATGGGATTGTATTGCAGATTGACCAACCTTACCCTTCCAATGAGGAAAAACTAGTCAGAAACACAAGGCAAAACCAGGCAGAGGCACACAGTGAAGTGAAAGAGAAGTTTCAATTTTTCACTGAAGTGCCATCTGGTTCTCAACAGAAGTACAAAGATGTGGCGGATGCAGCGCAAGCCGCTTTCGAATCCGCAGCTCAAGCAGCAGCTGCTGCCCGAGCTGCCATGGAGCTCTCCCGCTCCCATGATGGTCCAAGCAGCCCGAATAGCCCTGGCTCTGGAACAACTCCACATAACAAACAAGAGGTAGAGGAACCAAAGGTTGAATCAAAACCAAAACCAAAAATGGAATATGGAATTCGGAGAGAAGGAGAAGGAGAAGGAGAAGGAGAAGAAGAAGTGAAAAATTCAGTGCCTGGTGCTTCTTCTCCAAGTAGAGAGTTCTGTTATGGGGAAAAGGAAAAGAGTGACATGGAAACAAAGTTAGAGAAGACAGAGTTAAGTGAGAAGGCAAGCTTTCGTTTAAATCTGGAGAAGCAGCCAATTTCAGTGAGAACGAGAAGAGTGCGAGGATACTGAAAAATATATGTAGTTCAGAATTGGGTTTGCTTTTGTATTCCTTCTACATATGTATAATTTTATTTTTGGTTTGGAAAGATCATGAAAATTGTGTAATTATGTGTGTGCAAATATTGGAATTTTATTGTTAAATTCGTTACTTTACAATGAAAACAATACGTGGGTTAATATACAAAATGTA

Coding sequence (CDS)

ATGGGAAAAAAGCTTGACGCTTTTCTTGGTAGGAATTTCAGAGCCTCCAAATTCCGTCCCCTTCTCAGTCTTGCCGTCACTCGCCTTTCCATCCTCACTACCCAACGCCGTCTCAGATGCTCTCAGGCTCATTCCGACGTCCTTCAACTCCTCCAACTTCCCCACCACCACCGTGCTCTTCTTCGAGTCGAGAAAGTGATTGTGGATCAGAATGCTCTGGATGCCTATGTTTTGATCGAAGGGTATCTCAACCTCTTGCTTGAAAGAACCACCCTCCTCGAACAACAAAGTGAGTGCCCTGAGGAATTGAAAGAAGCGGTTGCAGGGTTGATATTTGCTGCTTCCAGATGTGGAGATTTCCCTGAACTTCATGAGATCAAATCAGTTTTGACCAATCGTTTTGGCAAGGAATTCACTGCTCGTGCCGTTGAATTACGCAACAATTGTGGAGTCAATCATTTGTTAATGCAAAAACTTTCCACAAGGCAGCCCAGTTTGGAGAGTAGAATGGAGGTCCTCAAAGCCATTGCTTCTGAGAATGGGATTGTATTGCAGATTGACCAACCTTACCCTTCCAATGAGGAAAAACTAGTCAGAAACACAAGGCAAAACCAGGCAGAGGCACACAGTGAAGTGAAAGAGAAGTTTCAATTTTTCACTGAAGTGCCATCTGGTTCTCAACAGAAGTACAAAGATGTGGCGGATGCAGCGCAAGCCGCTTTCGAATCCGCAGCTCAAGCAGCAGCTGCTGCCCGAGCTGCCATGGAGCTCTCCCGCTCCCATGATGGTCCAAGCAGCCCGAATAGCCCTGGCTCTGGAACAACTCCACATAACAAACAAGAGGTAGAGGAACCAAAGGTTGAATCAAAACCAAAACCAAAAATGGAATATGGAATTCGGAGAGAAGGAGAAGGAGAAGGAGAAGGAGAAGAAGAAGTGAAAAATTCAGTGCCTGGTGCTTCTTCTCCAAGTAGAGAGTTCTGTTATGGGGAAAAGGAAAAGAGTGACATGGAAACAAAGTTAGAGAAGACAGAGTTAAGTGAGAAGGCAAGCTTTCGTTTAAATCTGGAGAAGCAGCCAATTTCAGTGAGAACGAGAAGAGTGCGAGGATACTGA

Protein sequence

MGKKLDAFLGRNFRASKFRPLLSLAVTRLSILTTQRRLRCSQAHSDVLQLLQLPHHHRALLRVEKVIVDQNALDAYVLIEGYLNLLLERTTLLEQQSECPEELKEAVAGLIFAASRCGDFPELHEIKSVLTNRFGKEFTARAVELRNNCGVNHLLMQKLSTRQPSLESRMEVLKAIASENGIVLQIDQPYPSNEEKLVRNTRQNQAEAHSEVKEKFQFFTEVPSGSQQKYKDVADAAQAAFESAAQAAAAARAAMELSRSHDGPSSPNSPGSGTTPHNKQEVEEPKVESKPKPKMEYGIRREGEGEGEGEEEVKNSVPGASSPSREFCYGEKEKSDMETKLEKTELSEKASFRLNLEKQPISVRTRRVRGY
Homology
BLAST of ClCG08G005190 vs. NCBI nr
Match: XP_038884116.1 (uncharacterized protein LOC120075040 [Benincasa hispida])

HSP 1 Score: 536.6 bits (1381), Expect = 1.7e-148
Identity = 299/371 (80.59%), Postives = 322/371 (86.79%), Query Frame = 0

Query: 1   MGKKLDAFLGRNFRASKFRPLLSLAVTRLSILTTQRRLRCSQAHSDVLQLLQLPHHHRAL 60
           MG+KLDA LGRNFRASKFRPLL+LA++RLSILT QRR+RCSQA+SDVLQLLQLP+HHRAL
Sbjct: 1   MGRKLDALLGRNFRASKFRPLLNLALSRLSILTAQRRVRCSQANSDVLQLLQLPNHHRAL 60

Query: 61  LRVEKVIVDQNALDAYVLIEGYLNLLLERTTLLEQQSECPEELKEAVAGLIFAASRCGDF 120
           LRVEKVI DQNALDAYVLIEGYLNLLLERTTLLEQQSECPEELKEAVAGL+FAASRCGDF
Sbjct: 61  LRVEKVIKDQNALDAYVLIEGYLNLLLERTTLLEQQSECPEELKEAVAGLLFAASRCGDF 120

Query: 121 PELHEIKSVLTNRFGKEFTARAVELRNNCGVNHLLMQKLSTRQPSLESRMEVLKAIASEN 180
           PELHEIKSVLT RFGKEFTARAVELRNNCGVNHLLMQKLSTRQP+LESRMEVLKAIASEN
Sbjct: 121 PELHEIKSVLTTRFGKEFTARAVELRNNCGVNHLLMQKLSTRQPTLESRMEVLKAIASEN 180

Query: 181 GIVLQIDQPYPSNEEKLVRNTRQNQAEAHSEVKEKFQFFTEVPSGSQQKYKDVADAAQAA 240
           GIVLQID   PSNEEKL RNTRQNQ E  SEV E  QF TEV SGS+QKYKDVADAAQAA
Sbjct: 181 GIVLQIDNHPPSNEEKLTRNTRQNQPEPRSEVVENLQFSTEVASGSKQKYKDVADAAQAA 240

Query: 241 FESAAQAAAAARAAMELSRSHDGPSSPNSPGSGTTPHNKQEVEEPKVESKPKPKMEYGIR 300
           FESAAQAAAAARAAMELSRSHDGPSSP SPGSGTTPH+KQE+E+ +VESKP P+ E   R
Sbjct: 241 FESAAQAAAAARAAMELSRSHDGPSSPKSPGSGTTPHDKQELEKAEVESKPNPETENRNR 300

Query: 301 REGEGEGEGEEEVKNSVPGASSPSREFCYGEKEKSDMETKLEKTELSEKASFRLNLEKQP 360
           R G G  E EEE + +         E     K   ++E K+EKTE+SEK SFRLNLEK+P
Sbjct: 301 RGGGGGEEEEEEEEGNKMRGDEMDEERGSKVKNGLNVERKVEKTEVSEKGSFRLNLEKKP 360

Query: 361 ISVRTRRVRGY 372
           ISVRTRRVRG+
Sbjct: 361 ISVRTRRVRGH 371

BLAST of ClCG08G005190 vs. NCBI nr
Match: XP_011656519.1 (uncharacterized protein LOC101211044 [Cucumis sativus] >KGN45949.1 hypothetical protein Csa_005294 [Cucumis sativus])

HSP 1 Score: 486.5 bits (1251), Expect = 2.1e-133
Identity = 282/372 (75.81%), Postives = 310/372 (83.33%), Query Frame = 0

Query: 1   MGKKLDAFLGRNFRASKFRPLLSLAVTRLSILTTQRRLRCSQAHSDVLQLLQLPHHHRAL 60
           MG+KLDA LGRNFRASKFRPLL+L+++RLSILT QRR+ CSQA+SDVLQLLQL HHHRAL
Sbjct: 1   MGRKLDALLGRNFRASKFRPLLNLSLSRLSILTAQRRVGCSQANSDVLQLLQLSHHHRAL 60

Query: 61  LRVEKVIVDQNALDAYVLIEGYLNLLLERTTLLEQQSECPEELKEAVAGLIFAASRCGDF 120
           LRVEKVI DQNALDAYVLIEGYLNLLLERTTLLEQQSECPEELKEAVAGL+FAASRCGDF
Sbjct: 61  LRVEKVIKDQNALDAYVLIEGYLNLLLERTTLLEQQSECPEELKEAVAGLLFAASRCGDF 120

Query: 121 PELHEIKSVLTNRFGKEFTARAVELRNNCGVNHLLMQKLSTRQPSLESRMEVLKAIASEN 180
           PELHEIKSVLT RFGKEFTARAVELRNNCGVN  LMQKLSTRQP+LE+RM+ LK+IASEN
Sbjct: 121 PELHEIKSVLTTRFGKEFTARAVELRNNCGVNLSLMQKLSTRQPTLETRMDALKSIASEN 180

Query: 181 GIVLQIDQPYPSNEEKLVRNTRQNQAEAHSEVKEKFQFFTEVPSGSQQKYKDVADAAQAA 240
           GIVLQIDQ   S +EK+ RN RQ++AE  SE     +F  EV SGS+  YKDVADAAQAA
Sbjct: 181 GIVLQIDQLPSSKQEKVGRNGRQSEAEGQSE-----EFSNEVASGSKTTYKDVADAAQAA 240

Query: 241 FESAAQAAAAARAAMELSRSHDGPSSPNSPGSGTTPHNKQEVEEPKVESKPKPKM-EYGI 300
           FESAAQAAAAARAAMELSRSH+GPSSP+ PGSGTT  NKQ+ E+ +VESK K +M EYG 
Sbjct: 241 FESAAQAAAAARAAMELSRSHEGPSSPSKPGSGTTSDNKQKKEKREVESKVKQEMEEYGN 300

Query: 301 RREGEGEGEGEEEVKNSVPGASSPSREFCYGEKEKSDMETKLEKTELSEKASFRLNLEKQ 360
            R+GEGEGE EE       G          G K    METK+EKTE+SEK SFRLNLEK+
Sbjct: 301 GRKGEGEGEEEE-------GDGMDEERTSNGLK----METKVEKTEVSEKGSFRLNLEKK 356

Query: 361 PISVRTRRVRGY 372
           PISVRTRRV GY
Sbjct: 361 PISVRTRRVGGY 356

BLAST of ClCG08G005190 vs. NCBI nr
Match: XP_022921602.1 (uncharacterized protein LOC111429814 [Cucurbita moschata] >KAG7032508.1 IST1-like protein, partial [Cucurbita argyrosperma subsp. argyrosperma])

HSP 1 Score: 474.2 bits (1219), Expect = 1.1e-129
Identity = 278/388 (71.65%), Postives = 314/388 (80.93%), Query Frame = 0

Query: 1   MGKKLDAFLGRNFRASKFRPLLSLAVTRLSILTTQRRLRCSQAHSDVLQLLQLPHHHRAL 60
           MG+ LDA LGRNFRASKFRPLL+LA++RL+ILT QRRLR SQA SDVLQLLQL H  RAL
Sbjct: 1   MGRTLDALLGRNFRASKFRPLLNLALSRLAILTNQRRLRRSQAQSDVLQLLQLHHQQRAL 60

Query: 61  LRVEKVIVDQNALDAYVLIEGYLNLLLERTTLLEQQSECPEELKEAVAGLIFAASRCGDF 120
           LRVE+VI DQNALDAYVLIEGYLNLL+ERT+LLEQQ +CPEELKEA+AGL+FAASRCGDF
Sbjct: 61  LRVEQVIKDQNALDAYVLIEGYLNLLIERTSLLEQQRDCPEELKEAIAGLLFAASRCGDF 120

Query: 121 PELHEIKSVLTNRFGKEFTARAVELRNNCGVNHLLMQKLSTRQPSLESRMEVLKAIASEN 180
           PELHEIKSVLT+RFGKEFTARAVELRNNCGVNHLLMQKLSTR P+LESRM++LK IASEN
Sbjct: 121 PELHEIKSVLTSRFGKEFTARAVELRNNCGVNHLLMQKLSTRHPNLESRMDLLKTIASEN 180

Query: 181 GIVLQIDQPYPSNEEKLVRNTRQNQAEAHSEVKEKFQFFTEVPSGSQQKYKDVADAAQAA 240
           GI LQ+D+   SNE    RN+RQNQ+E H  V E  +F TEVPSGS+QKYKDVADAAQAA
Sbjct: 181 GITLQLDETPLSNE---ARNSRQNQSEPHGGVGENLKFSTEVPSGSKQKYKDVADAAQAA 240

Query: 241 FESAAQAAAAARAAMELSRSH----DGPSSPNSPGSGTTPHNKQEVEEPKVESKPKPKME 300
           FESAAQAAAAARAAMELSRS     DGPSSP  PGS TT ++KQE ++ +VE+KPK ++E
Sbjct: 241 FESAAQAAAAARAAMELSRSESQDPDGPSSP-GPGSATTSYHKQEQKKSEVEAKPKSEIE 300

Query: 301 YGIRREGEGEG-EGEEEVKNSVPGASSPSREFCYGEKEKSD------------METKLEK 360
           Y   +E EG     E EVKNS+ G SS +RE    E  + D            ME  LEK
Sbjct: 301 YEYGKEEEGSKIRAEAEVKNSMCGCSS-NRELFVKENREMDEQRATNIEIGEEMEANLEK 360

Query: 361 TELSEKASFRLNLEKQPISVRTRRVRGY 372
           TE++EK SFRLNLEK+PISVRTRRVRGY
Sbjct: 361 TEITEKPSFRLNLEKKPISVRTRRVRGY 383

BLAST of ClCG08G005190 vs. NCBI nr
Match: KAG6601794.1 (IST1-like protein, partial [Cucurbita argyrosperma subsp. sororia])

HSP 1 Score: 472.6 bits (1215), Expect = 3.1e-129
Identity = 278/388 (71.65%), Postives = 313/388 (80.67%), Query Frame = 0

Query: 1   MGKKLDAFLGRNFRASKFRPLLSLAVTRLSILTTQRRLRCSQAHSDVLQLLQLPHHHRAL 60
           MG+ LDA LGRNFRASKFRPLL+LAV+RL+ILT QRRLR SQA SDVLQLLQL H  RAL
Sbjct: 1   MGRTLDALLGRNFRASKFRPLLNLAVSRLAILTNQRRLRRSQAQSDVLQLLQLHHQQRAL 60

Query: 61  LRVEKVIVDQNALDAYVLIEGYLNLLLERTTLLEQQSECPEELKEAVAGLIFAASRCGDF 120
           LRVE+VI DQNALDAYVLIEGYLNLL+ER +LLEQ+ ECPEELKEA+AGL+FAASRCGDF
Sbjct: 61  LRVEQVIKDQNALDAYVLIEGYLNLLIERASLLEQERECPEELKEALAGLLFAASRCGDF 120

Query: 121 PELHEIKSVLTNRFGKEFTARAVELRNNCGVNHLLMQKLSTRQPSLESRMEVLKAIASEN 180
           PELHEIKSVLT+RFGKEFTARAVELRNNCGVNHLLMQKLSTR P+LESRM++LK IASEN
Sbjct: 121 PELHEIKSVLTSRFGKEFTARAVELRNNCGVNHLLMQKLSTRHPNLESRMDLLKTIASEN 180

Query: 181 GIVLQIDQPYPSNEEKLVRNTRQNQAEAHSEVKEKFQFFTEVPSGSQQKYKDVADAAQAA 240
           GI LQ+D+   SNE    RN+RQNQ+E H  V E  +F TEVPSGS+QKYKDVADAAQAA
Sbjct: 181 GITLQLDETPLSNE---ARNSRQNQSEPHGGVGENLKFSTEVPSGSKQKYKDVADAAQAA 240

Query: 241 FESAAQAAAAARAAMELSRSH----DGPSSPNSPGSGTTPHNKQEVEEPKVESKPKPKME 300
           FESAAQAAAAARAAMELSRS     DGPSSP  PGS TT ++KQE ++ +VE+KPK ++E
Sbjct: 241 FESAAQAAAAARAAMELSRSESQDPDGPSSP-GPGSATTSYHKQEQKKSEVEAKPKSEIE 300

Query: 301 YGIRREGEGEG-EGEEEVKNSVPGASSPSREFCYGEKEKSD------------METKLEK 360
           Y   +E EG     E EVKNS+ G SS +RE    E  + D            ME  LEK
Sbjct: 301 YEYGKEEEGSKIRAEAEVKNSMCGCSS-NRELFVKENREMDEQRATNIEIGEEMEANLEK 360

Query: 361 TELSEKASFRLNLEKQPISVRTRRVRGY 372
           TE++EK SFRLNLEK+PISVRTRRVRGY
Sbjct: 361 TEITEKPSFRLNLEKKPISVRTRRVRGY 383

BLAST of ClCG08G005190 vs. NCBI nr
Match: XP_023538782.1 (uncharacterized protein LOC111799606 [Cucurbita pepo subsp. pepo])

HSP 1 Score: 468.8 bits (1205), Expect = 4.4e-128
Identity = 277/388 (71.39%), Postives = 311/388 (80.15%), Query Frame = 0

Query: 1   MGKKLDAFLGRNFRASKFRPLLSLAVTRLSILTTQRRLRCSQAHSDVLQLLQLPHHHRAL 60
           MG+ LDA LGRNFRASKFRPLL+LA++RL+ILT QRRLR SQA SDVLQLLQL H  RAL
Sbjct: 1   MGRTLDALLGRNFRASKFRPLLNLALSRLAILTNQRRLRRSQAQSDVLQLLQLRHQQRAL 60

Query: 61  LRVEKVIVDQNALDAYVLIEGYLNLLLERTTLLEQQSECPEELKEAVAGLIFAASRCGDF 120
           LRVE+VI DQNALDAYVLIEGYLNLL+ERT+LLEQ+ ECPEELKEA+AGL+FAASRCGDF
Sbjct: 61  LRVEQVIKDQNALDAYVLIEGYLNLLIERTSLLEQERECPEELKEAIAGLLFAASRCGDF 120

Query: 121 PELHEIKSVLTNRFGKEFTARAVELRNNCGVNHLLMQKLSTRQPSLESRMEVLKAIASEN 180
           PELHEIKSVLT+RFGKEFTARAVELRNNCGVNHLLMQKLSTRQP+LESRM++LK IASEN
Sbjct: 121 PELHEIKSVLTSRFGKEFTARAVELRNNCGVNHLLMQKLSTRQPNLESRMDLLKTIASEN 180

Query: 181 GIVLQIDQPYPSNEEKLVRNTRQNQAEAHSEVKEKFQFFTEVPSGSQQKYKDVADAAQAA 240
           GI LQ+D+   SNE    RN+ QNQ E H  V E  +  TEVPSGS+QKYKDVADAAQAA
Sbjct: 181 GITLQLDETPLSNE---ARNSSQNQPEPHGVVGENLKLSTEVPSGSKQKYKDVADAAQAA 240

Query: 241 FESAAQAAAAARAAMELSRSH----DGPSSPNSPGSGTTPHNKQEVEEPKVESKPKPKME 300
           FESAAQAAAAARAAMELSR      DGPSSP  PGSGTT ++KQE ++  VE+KPK ++E
Sbjct: 241 FESAAQAAAAARAAMELSRPESQDPDGPSSP-GPGSGTTSYDKQEQKKSDVEAKPKLEIE 300

Query: 301 YGIRREGEGEG-EGEEEVKNSVPGASSPSREFCYGEKEKSD------------METKLEK 360
           Y   RE EG     E EVKNS+ G SS +RE    E  + D            ME  LEK
Sbjct: 301 YEYGREEEGSKIRAEAEVKNSMCGCSS-NRELFVKENREMDEQRASNIEIGEEMEANLEK 360

Query: 361 TELSEKASFRLNLEKQPISVRTRRVRGY 372
           TE++EK SFRLNLEK+PISVRTRRVRG+
Sbjct: 361 TEITEKPSFRLNLEKKPISVRTRRVRGH 383

BLAST of ClCG08G005190 vs. ExPASy Swiss-Prot
Match: Q54I39 (IST1-like protein OS=Dictyostelium discoideum OX=44689 GN=DDB_G0289029 PE=3 SV=1)

HSP 1 Score: 69.7 bits (169), Expect = 7.8e-11
Identity = 51/174 (29.31%), Postives = 88/174 (50.57%), Query Frame = 0

Query: 10  GRNFRASKFRPLLSLAVTRLSILTTQRRLRCSQAHSDVLQLLQLPHHHRALLRVEKVIVD 69
           G ++ + K +  L LAV+R+ IL  ++         +V +LL+  +   A +RVE +I D
Sbjct: 5   GPSYDSYKLKVQLKLAVSRIQILKNKKANIVRDEKRNVAELLRKKNEESARIRVETIIRD 64

Query: 70  QNALDAYVLIEGYLNLLLERTTLLEQQSECPEELKEAVAGLIFAASRCGDFPELHEIKSV 129
           +  ++ + +IE    LL  R  L+   +E P E+KE++  L++++ R    PEL +IK+ 
Sbjct: 65  EYLIECFQIIEVLCELLHARINLINATTEMPLEMKESIFTLVYSSQRI-QIPELEQIKNQ 124

Query: 130 LTNRFGKEFTARAVELRNNC----GVNHLLMQKLSTRQPSLESRMEVLKAIASE 180
           L  ++GK      +E   NC     VN  ++ KLS   P      + L  IA +
Sbjct: 125 LKAKYGK-----GLENEANCHCSTHVNPKIVHKLSYATPDPSIIFQTLSEIAEK 172

BLAST of ClCG08G005190 vs. ExPASy Swiss-Prot
Match: P53990 (IST1 homolog OS=Homo sapiens OX=9606 GN=IST1 PE=1 SV=1)

HSP 1 Score: 66.6 bits (161), Expect = 6.6e-10
Identity = 48/157 (30.57%), Postives = 76/157 (48.41%), Query Frame = 0

Query: 9   LGRNFRASKFRPLLSLAVTRLSILTTQRRLRCSQAHSDVLQLLQLPHHHRALLRVEKVIV 68
           LG  F+A + R  L L + RL +L  ++     +A  ++   L      RA +RVE +I 
Sbjct: 2   LGSGFKAERLRVNLRLVINRLKLLEKKKTELAQKARKEIADYLAAGKDERARIRVEHIIR 61

Query: 69  DQNALDAYVLIEGYLNLLLERTTLLEQQSECPEELKEAVAGLIFAASRC-GDFPELHEIK 128
           +   ++A  ++E Y +LLL R  L++   E    L E+V+ LI+AA R   +  EL  + 
Sbjct: 62  EDYLVEAMEILELYCDLLLARFGLIQSMKELDSGLAESVSTLIWAAPRLQSEVAELKIVA 121

Query: 129 SVLTNRFGKEFTARAVELRNNCGVNHLLMQKLSTRQP 165
             L  ++ KE+  +         VN  LM KLS   P
Sbjct: 122 DQLCAKYSKEY-GKLCRTNQIGTVNDRLMHKLSVEAP 157

BLAST of ClCG08G005190 vs. ExPASy Swiss-Prot
Match: Q9CX00 (IST1 homolog OS=Mus musculus OX=10090 GN=Ist1 PE=1 SV=1)

HSP 1 Score: 66.6 bits (161), Expect = 6.6e-10
Identity = 48/157 (30.57%), Postives = 76/157 (48.41%), Query Frame = 0

Query: 9   LGRNFRASKFRPLLSLAVTRLSILTTQRRLRCSQAHSDVLQLLQLPHHHRALLRVEKVIV 68
           LG  F+A + R  L L + RL +L  ++     +A  ++   L      RA +RVE +I 
Sbjct: 2   LGSGFKAERLRVNLRLVINRLKLLEKKKTELAQKARKEIADYLAAGKDERARIRVEHIIR 61

Query: 69  DQNALDAYVLIEGYLNLLLERTTLLEQQSECPEELKEAVAGLIFAASRC-GDFPELHEIK 128
           +   ++A  ++E Y +LLL R  L++   E    L E+V+ LI+AA R   +  EL  + 
Sbjct: 62  EDYLVEAMEILELYCDLLLARFGLIQSMKELDSGLAESVSTLIWAAPRLQSEVAELKIVA 121

Query: 129 SVLTNRFGKEFTARAVELRNNCGVNHLLMQKLSTRQP 165
             L  ++ KE+  +         VN  LM KLS   P
Sbjct: 122 DQLCAKYSKEY-GKLCRTNQIGTVNDRLMHKLSVEAP 157

BLAST of ClCG08G005190 vs. ExPASy Swiss-Prot
Match: Q568Z6 (IST1 homolog OS=Rattus norvegicus OX=10116 GN=Ist1 PE=2 SV=1)

HSP 1 Score: 66.6 bits (161), Expect = 6.6e-10
Identity = 48/157 (30.57%), Postives = 76/157 (48.41%), Query Frame = 0

Query: 9   LGRNFRASKFRPLLSLAVTRLSILTTQRRLRCSQAHSDVLQLLQLPHHHRALLRVEKVIV 68
           LG  F+A + R  L L + RL +L  ++     +A  ++   L      RA +RVE +I 
Sbjct: 2   LGSGFKAERLRVNLRLVINRLKLLEKKKTELAQKARKEIADYLAAGKDERARIRVEHIIR 61

Query: 69  DQNALDAYVLIEGYLNLLLERTTLLEQQSECPEELKEAVAGLIFAASRC-GDFPELHEIK 128
           +   ++A  ++E Y +LLL R  L++   E    L E+V+ LI+AA R   +  EL  + 
Sbjct: 62  EDYLVEAMEILELYCDLLLARFGLIQSMKELDSGLAESVSTLIWAAPRLQSEVAELKIVA 121

Query: 129 SVLTNRFGKEFTARAVELRNNCGVNHLLMQKLSTRQP 165
             L  ++ KE+  +         VN  LM KLS   P
Sbjct: 122 DQLCAKYSKEY-GKLCRTNQIGTVNDRLMHKLSVEAP 157

BLAST of ClCG08G005190 vs. ExPASy Swiss-Prot
Match: Q3ZBV1 (IST1 homolog OS=Bos taurus OX=9913 GN=IST1 PE=2 SV=1)

HSP 1 Score: 64.3 bits (155), Expect = 3.3e-09
Identity = 47/157 (29.94%), Postives = 75/157 (47.77%), Query Frame = 0

Query: 9   LGRNFRASKFRPLLSLAVTRLSILTTQRRLRCSQAHSDVLQLLQLPHHHRALLRVEKVIV 68
           LG   +A + R  L L + RL +L  ++     +A  ++   L      RA +RVE +I 
Sbjct: 2   LGSGIKAERLRVNLRLVINRLKLLEKKKTELAQKARKEIADYLAAGKDERARIRVEHIIR 61

Query: 69  DQNALDAYVLIEGYLNLLLERTTLLEQQSECPEELKEAVAGLIFAASRC-GDFPELHEIK 128
           +   ++A  ++E Y +LLL R  L++   E    L E+V+ LI+AA R   +  EL  + 
Sbjct: 62  EDYLVEAMEILELYCDLLLARFGLIQSMKELDSGLAESVSTLIWAAPRLQSEVAELKIVA 121

Query: 129 SVLTNRFGKEFTARAVELRNNCGVNHLLMQKLSTRQP 165
             L  ++ KE+  +         VN  LM KLS   P
Sbjct: 122 DQLCAKYSKEY-GKLCRTNQIGTVNDRLMHKLSVEAP 157

BLAST of ClCG08G005190 vs. ExPASy TrEMBL
Match: A0A0A0KAU0 (Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_6G032470 PE=3 SV=1)

HSP 1 Score: 486.5 bits (1251), Expect = 9.9e-134
Identity = 282/372 (75.81%), Postives = 310/372 (83.33%), Query Frame = 0

Query: 1   MGKKLDAFLGRNFRASKFRPLLSLAVTRLSILTTQRRLRCSQAHSDVLQLLQLPHHHRAL 60
           MG+KLDA LGRNFRASKFRPLL+L+++RLSILT QRR+ CSQA+SDVLQLLQL HHHRAL
Sbjct: 1   MGRKLDALLGRNFRASKFRPLLNLSLSRLSILTAQRRVGCSQANSDVLQLLQLSHHHRAL 60

Query: 61  LRVEKVIVDQNALDAYVLIEGYLNLLLERTTLLEQQSECPEELKEAVAGLIFAASRCGDF 120
           LRVEKVI DQNALDAYVLIEGYLNLLLERTTLLEQQSECPEELKEAVAGL+FAASRCGDF
Sbjct: 61  LRVEKVIKDQNALDAYVLIEGYLNLLLERTTLLEQQSECPEELKEAVAGLLFAASRCGDF 120

Query: 121 PELHEIKSVLTNRFGKEFTARAVELRNNCGVNHLLMQKLSTRQPSLESRMEVLKAIASEN 180
           PELHEIKSVLT RFGKEFTARAVELRNNCGVN  LMQKLSTRQP+LE+RM+ LK+IASEN
Sbjct: 121 PELHEIKSVLTTRFGKEFTARAVELRNNCGVNLSLMQKLSTRQPTLETRMDALKSIASEN 180

Query: 181 GIVLQIDQPYPSNEEKLVRNTRQNQAEAHSEVKEKFQFFTEVPSGSQQKYKDVADAAQAA 240
           GIVLQIDQ   S +EK+ RN RQ++AE  SE     +F  EV SGS+  YKDVADAAQAA
Sbjct: 181 GIVLQIDQLPSSKQEKVGRNGRQSEAEGQSE-----EFSNEVASGSKTTYKDVADAAQAA 240

Query: 241 FESAAQAAAAARAAMELSRSHDGPSSPNSPGSGTTPHNKQEVEEPKVESKPKPKM-EYGI 300
           FESAAQAAAAARAAMELSRSH+GPSSP+ PGSGTT  NKQ+ E+ +VESK K +M EYG 
Sbjct: 241 FESAAQAAAAARAAMELSRSHEGPSSPSKPGSGTTSDNKQKKEKREVESKVKQEMEEYGN 300

Query: 301 RREGEGEGEGEEEVKNSVPGASSPSREFCYGEKEKSDMETKLEKTELSEKASFRLNLEKQ 360
            R+GEGEGE EE       G          G K    METK+EKTE+SEK SFRLNLEK+
Sbjct: 301 GRKGEGEGEEEE-------GDGMDEERTSNGLK----METKVEKTEVSEKGSFRLNLEKK 356

Query: 361 PISVRTRRVRGY 372
           PISVRTRRV GY
Sbjct: 361 PISVRTRRVGGY 356

BLAST of ClCG08G005190 vs. ExPASy TrEMBL
Match: A0A6J1E0Z0 (uncharacterized protein LOC111429814 OS=Cucurbita moschata OX=3662 GN=LOC111429814 PE=3 SV=1)

HSP 1 Score: 474.2 bits (1219), Expect = 5.1e-130
Identity = 278/388 (71.65%), Postives = 314/388 (80.93%), Query Frame = 0

Query: 1   MGKKLDAFLGRNFRASKFRPLLSLAVTRLSILTTQRRLRCSQAHSDVLQLLQLPHHHRAL 60
           MG+ LDA LGRNFRASKFRPLL+LA++RL+ILT QRRLR SQA SDVLQLLQL H  RAL
Sbjct: 1   MGRTLDALLGRNFRASKFRPLLNLALSRLAILTNQRRLRRSQAQSDVLQLLQLHHQQRAL 60

Query: 61  LRVEKVIVDQNALDAYVLIEGYLNLLLERTTLLEQQSECPEELKEAVAGLIFAASRCGDF 120
           LRVE+VI DQNALDAYVLIEGYLNLL+ERT+LLEQQ +CPEELKEA+AGL+FAASRCGDF
Sbjct: 61  LRVEQVIKDQNALDAYVLIEGYLNLLIERTSLLEQQRDCPEELKEAIAGLLFAASRCGDF 120

Query: 121 PELHEIKSVLTNRFGKEFTARAVELRNNCGVNHLLMQKLSTRQPSLESRMEVLKAIASEN 180
           PELHEIKSVLT+RFGKEFTARAVELRNNCGVNHLLMQKLSTR P+LESRM++LK IASEN
Sbjct: 121 PELHEIKSVLTSRFGKEFTARAVELRNNCGVNHLLMQKLSTRHPNLESRMDLLKTIASEN 180

Query: 181 GIVLQIDQPYPSNEEKLVRNTRQNQAEAHSEVKEKFQFFTEVPSGSQQKYKDVADAAQAA 240
           GI LQ+D+   SNE    RN+RQNQ+E H  V E  +F TEVPSGS+QKYKDVADAAQAA
Sbjct: 181 GITLQLDETPLSNE---ARNSRQNQSEPHGGVGENLKFSTEVPSGSKQKYKDVADAAQAA 240

Query: 241 FESAAQAAAAARAAMELSRSH----DGPSSPNSPGSGTTPHNKQEVEEPKVESKPKPKME 300
           FESAAQAAAAARAAMELSRS     DGPSSP  PGS TT ++KQE ++ +VE+KPK ++E
Sbjct: 241 FESAAQAAAAARAAMELSRSESQDPDGPSSP-GPGSATTSYHKQEQKKSEVEAKPKSEIE 300

Query: 301 YGIRREGEGEG-EGEEEVKNSVPGASSPSREFCYGEKEKSD------------METKLEK 360
           Y   +E EG     E EVKNS+ G SS +RE    E  + D            ME  LEK
Sbjct: 301 YEYGKEEEGSKIRAEAEVKNSMCGCSS-NRELFVKENREMDEQRATNIEIGEEMEANLEK 360

Query: 361 TELSEKASFRLNLEKQPISVRTRRVRGY 372
           TE++EK SFRLNLEK+PISVRTRRVRGY
Sbjct: 361 TEITEKPSFRLNLEKKPISVRTRRVRGY 383

BLAST of ClCG08G005190 vs. ExPASy TrEMBL
Match: A0A6J1I2V3 (uncharacterized protein LOC111470447 OS=Cucurbita maxima OX=3661 GN=LOC111470447 PE=3 SV=1)

HSP 1 Score: 464.2 bits (1193), Expect = 5.3e-127
Identity = 274/388 (70.62%), Postives = 310/388 (79.90%), Query Frame = 0

Query: 1   MGKKLDAFLGRNFRASKFRPLLSLAVTRLSILTTQRRLRCSQAHSDVLQLLQLPHHHRAL 60
           MG+ LDA LGRNFRASKFRPLL+LAV+RL+ILT QRRLR +QA SDVLQLLQL H  RAL
Sbjct: 1   MGRTLDALLGRNFRASKFRPLLNLAVSRLAILTNQRRLRRTQAQSDVLQLLQLHHQQRAL 60

Query: 61  LRVEKVIVDQNALDAYVLIEGYLNLLLERTTLLEQQSECPEELKEAVAGLIFAASRCGDF 120
           LRVE+VI DQNALDAYVL+EGYLNLL+ERT+LLEQ+ ECPEELKEA+AGL+FAASRCGDF
Sbjct: 61  LRVEQVIKDQNALDAYVLMEGYLNLLIERTSLLEQERECPEELKEAIAGLLFAASRCGDF 120

Query: 121 PELHEIKSVLTNRFGKEFTARAVELRNNCGVNHLLMQKLSTRQPSLESRMEVLKAIASEN 180
           PELHEIKSVLT  FGKEFTARAVELRNNCGVNHLLMQKLSTRQP+LESRM++LK IASEN
Sbjct: 121 PELHEIKSVLTTHFGKEFTARAVELRNNCGVNHLLMQKLSTRQPNLESRMDLLKTIASEN 180

Query: 181 GIVLQIDQPYPSNEEKLVRNTRQNQAEAHSEVKEKFQFFTEVPSGSQQKYKDVADAAQAA 240
           GI LQ+D+   SNE    RN+RQNQ+E H  V E  +  TEVPSGS+QKYKDVADAAQAA
Sbjct: 181 GITLQLDETPLSNE---ARNSRQNQSEPHGGVGENLKLSTEVPSGSKQKYKDVADAAQAA 240

Query: 241 FESAAQAAAAARAAMELSRSH----DGPSSPNSPGSGTTPHNKQEVEEPKVESKPKPKME 300
           FESAAQAAAAARAAMELSRS     DGPSSP  PGS T+ ++KQE ++  VE+KPK ++E
Sbjct: 241 FESAAQAAAAARAAMELSRSESQDPDGPSSP-GPGSATSSYDKQEQKKSNVEAKPKLEIE 300

Query: 301 YGIRREGEGEG-EGEEEVKNSVPGASSPSREFCYGEKEKSD------------METKLEK 360
           Y   RE EG     + EVKNS+ G SS +RE    E  + D            ME  LEK
Sbjct: 301 YEYGREEEGSKIRPKPEVKNSMCGCSS-NRELFVKENREMDEQRASNIEIGEEMEANLEK 360

Query: 361 TELSEKASFRLNLEKQPISVRTRRVRGY 372
           T ++EK SFRLNLEK+PISVRTRRVRGY
Sbjct: 361 TAITEKPSFRLNLEKKPISVRTRRVRGY 383

BLAST of ClCG08G005190 vs. ExPASy TrEMBL
Match: A0A1S3BD83 (uncharacterized protein LOC103488606 OS=Cucumis melo OX=3656 GN=LOC103488606 PE=3 SV=1)

HSP 1 Score: 456.4 bits (1173), Expect = 1.1e-124
Identity = 254/313 (81.15%), Postives = 280/313 (89.46%), Query Frame = 0

Query: 1   MGKKLDAFLGRNFRASKFRPLLSLAVTRLSILTTQRRLRCSQAHSDVLQLLQLPHHHRAL 60
           MG+KLDA LGRNFRASKFRPLL+L+++RLSILT+QRRLRCSQA+SDVLQLLQL HHHRAL
Sbjct: 1   MGRKLDALLGRNFRASKFRPLLNLSLSRLSILTSQRRLRCSQANSDVLQLLQLSHHHRAL 60

Query: 61  LRVEKVIVDQNALDAYVLIEGYLNLLLERTTLLEQQSECPEELKEAVAGLIFAASRCGDF 120
           LRVEKVI DQNALDAYVLIEGYLNLLLERTTLLEQQSECPEELKEAVAGL+FAASRCGDF
Sbjct: 61  LRVEKVIKDQNALDAYVLIEGYLNLLLERTTLLEQQSECPEELKEAVAGLLFAASRCGDF 120

Query: 121 PELHEIKSVLTNRFGKEFTARAVELRNNCGVNHLLMQKLSTRQPSLESRMEVLKAIASEN 180
           PELHEIKSVLT RFGKEFTARAVELRNNCGVN  LMQKLSTRQP+LE+RMEVLK+IASEN
Sbjct: 121 PELHEIKSVLTTRFGKEFTARAVELRNNCGVNLSLMQKLSTRQPTLETRMEVLKSIASEN 180

Query: 181 GIVLQIDQPYPSNEEKLVRNTRQNQAEAHSEVKEKFQFFTEVPSGSQQKYKDVADAAQAA 240
           GIVLQ DQ   SNEEK+ R+ RQ++AE  SE     +F  EV S S+++YKDVADAAQAA
Sbjct: 181 GIVLQFDQLPHSNEEKVARSGRQSEAEGRSE-----KFSNEVASDSKKRYKDVADAAQAA 240

Query: 241 FESAAQAAAAARAAMELSRSHDGPSSPNSPGSGTTPHNKQEVEEPKVESKPKPKM-EYGI 300
           FESAAQAAAAARAAMELSRSH+GPSSP+ PGSGTTPH+KQ+ E+ +VESK K +M EYG 
Sbjct: 241 FESAAQAAAAARAAMELSRSHEGPSSPSKPGSGTTPHDKQKKEKTEVESKVKQEMEEYGN 300

Query: 301 RREGEGEGEGEEE 313
            R GEGEGEGE E
Sbjct: 301 GRRGEGEGEGEGE 308

BLAST of ClCG08G005190 vs. ExPASy TrEMBL
Match: A0A6J1CFG6 (uncharacterized protein LOC111010325 OS=Momordica charantia OX=3673 GN=LOC111010325 PE=3 SV=1)

HSP 1 Score: 450.7 bits (1158), Expect = 6.0e-123
Identity = 269/389 (69.15%), Postives = 312/389 (80.21%), Query Frame = 0

Query: 1   MGKKLDAFLGRNFRASKFRPLLSLAVTRLSILTTQRRLRCSQAHSDVLQLLQLPHHHRAL 60
           MG+KLDA LGRNFRASKFRPLL+LA++RL++LT QR +R SQA SD LQLLQL HHHRAL
Sbjct: 1   MGRKLDALLGRNFRASKFRPLLNLALSRLAVLTNQRHVRRSQARSDALQLLQLGHHHRAL 60

Query: 61  LRVEKVIVDQNALDAYVLIEGYLNLLLERTTLLEQQSECPEELKEAVAGLIFAASRCGDF 120
           LRVE+VI +QNALDAYVLIEGYLNLL+ERT LLEQ+ ECPEELKEAV+GLIFAASRCGDF
Sbjct: 61  LRVEQVIKEQNALDAYVLIEGYLNLLIERTDLLEQERECPEELKEAVSGLIFAASRCGDF 120

Query: 121 PELHEIKSVLTNRFGKEFTARAVELRNNCGVNHLLMQKLSTRQPSLESRMEVLKAIASEN 180
           PEL EIKSVLT RFGKEFTARAVELRNNCGV+H +MQKLSTRQP+LESRM VL+AIASEN
Sbjct: 121 PELQEIKSVLTTRFGKEFTARAVELRNNCGVDHSIMQKLSTRQPNLESRMNVLEAIASEN 180

Query: 181 GIVLQIDQPYPSNEEKLVRNTRQN-QAEAHSEVKEKFQFFTEVPSGS---QQKYKDVADA 240
            IVLQ+D+   SNEEK  RN RQN Q E  +EV +  QF  +VPSGS   +QKYKDVADA
Sbjct: 181 NIVLQLDEHPLSNEEKFARNRRQNYQQEPKAEVGDNLQFSADVPSGSMQAKQKYKDVADA 240

Query: 241 AQAAFESAAQAAAAARAAMELSRSH----DGPSSPNSPGSGTTPHNKQEVEEPKVESKPK 300
           AQAAFESAAQAAAAARAAMELSRS     D PS+P  PGSG+T + +QE ++ +VESKPK
Sbjct: 241 AQAAFESAAQAAAAARAAMELSRSQSQDPDDPSTP-KPGSGST-NKRQEQKKSEVESKPK 300

Query: 301 PKMEYGIRREGEGEGEG-EEEVKNSVPGASSPSREFCYGEKEKS---------DMETKLE 360
            ++EY  RRE E  G     EVKNS+P + SPSRE    E++++         + ETK E
Sbjct: 301 QEIEYQNRREEEEAGSKITAEVKNSMPASFSPSREDSEMEEQRAPNVETGLDMETETKPE 360

Query: 361 KTELSEKASFRLNLEKQPISVRTRRVRGY 372
            TE ++K SF LNLEK+P+SVRTRRVRG+
Sbjct: 361 LTEETQKPSFGLNLEKKPMSVRTRRVRGF 387

BLAST of ClCG08G005190 vs. TAIR 10
Match: AT1G13340.1 (Regulator of Vps4 activity in the MVB pathway protein )

HSP 1 Score: 243.4 bits (620), Expect = 2.8e-64
Identity = 158/322 (49.07%), Postives = 206/322 (63.98%), Query Frame = 0

Query: 1   MGKKLDAFLGRNFRASKFRPLLSLAVTRLSILTTQRRLRCSQAHSDVLQLLQLPHHHRAL 60
           MGKKLDA LGR+F+ +KF+ L++LA+TRLSIL  QR+ R SQA SDV +LL+L  H  A 
Sbjct: 1   MGKKLDALLGRSFKTNKFKSLITLALTRLSILKNQRQARLSQAISDVTELLKLGQHEHAY 60

Query: 61  LRVEKVIVDQNALDAYVLIEGYLNLLLERTTLLEQQSECPEELKEAVAGLIFAASRCGDF 120
            RV++V+ DQN LD    I GY  L L+R  L E   +CPEEL EAV+GL+FAASR G+F
Sbjct: 61  HRVDQVVKDQNTLDVLFFIHGYFTLCLDRIHLFEHNRDCPEELLEAVSGLLFAASRIGEF 120

Query: 121 PELHEIKSVLTNRFGKEFTARAVELRNNCGVNHLLMQKLSTRQPSLESRMEVLKAIASEN 180
           PEL EI++VL +RFGK+  AR++ELR+NCGV+  ++QKLSTR P  E RM+ LK IA+EN
Sbjct: 121 PELQEIRNVLISRFGKDLAARSIELRSNCGVDPKIIQKLSTRPPPKEVRMKALKEIAAEN 180

Query: 181 GIVLQIDQPYPSNEEKLVRNTRQNQAEAHSEVK----EKFQFFTEVPSGSQQKYKDVADA 240
            IVL++DQ   S E         + ++     K    E +     V  G ++KYKDVADA
Sbjct: 181 NIVLKLDQASTSTEGTTNMQGTSDVSKTKLTSKDGRGEGYGLSDSVKRG-KKKYKDVADA 240

Query: 241 AQAAFESAAQAAAAARAAMELSR-SHDGPSSPNSPGSGTTPHNKQEVEEPKVESKPKPKM 300
           AQAAFESAA AA AA+AA+ELS+ S  G  SP + G   + H          E+K   + 
Sbjct: 241 AQAAFESAAHAAEAAQAAVELSQFSPRGHDSPGNIGGENSFHGS--------ENKESEQE 300

Query: 301 EYGIRREGEGEGEGEEEVKNSV 318
           + G     EGEG+   E K S+
Sbjct: 301 QEGNDDSSEGEGDVMSESKRSM 313

BLAST of ClCG08G005190 vs. TAIR 10
Match: AT4G35730.1 (Regulator of Vps4 activity in the MVB pathway protein )

HSP 1 Score: 131.0 bits (328), Expect = 2.0e-30
Identity = 113/374 (30.21%), Postives = 175/374 (46.79%), Query Frame = 0

Query: 11  RNFRASKFRPLLSLAVTRLSILTTQRRLRCSQAHSDVLQLLQLPHHHRALLRVEKVIVDQ 70
           R F +SK +    +AV R+ ++  +R +   Q   D+  LLQ      A +RVE VI +Q
Sbjct: 24  RGFNSSKCKTAAKMAVARIKLIRNKRLVVVKQMRRDIAVLLQSGQDATARIRVEHVIREQ 83

Query: 71  NALDAYVLIEGYLNLLLERTTLLEQQSECPEELKEAVAGLIFAASRCGDFPELHEIKSVL 130
           N   A  +IE +  L++ R T++ +Q +CP +LKE +A LIFAA RC + PEL +++ + 
Sbjct: 84  NIQAANEIIELFCELIVSRLTIITKQKQCPVDLKEGIASLIFAAPRCSEIPELGDLRDIF 143

Query: 131 TNRFGKEFTARAVELRNNCGVNHLLMQKLSTRQPSLESRMEVLKAIASENGIVLQIDQPY 190
             ++GK+F + A +LR +CGVN +L+ KLS R P  E +++++K IA E     Q+D   
Sbjct: 144 AKKYGKDFVSAATDLRPSCGVNRMLIDKLSVRNPGGEYKLKIMKEIAKE----FQVDWDT 203

Query: 191 PSNEEKLVRNTRQN--------QAEA----HSEVKEKFQFFTEVPSGSQQ-----KYKDV 250
              E++L++   ++         A +     + + E       VP  +        Y D 
Sbjct: 204 TETEQELLKPQEESIDGPRKFVSASSLPVNRAAINEPIDPTKAVPRSTSSMSINTHYHDT 263

Query: 251 ADAAQAAFESAAQAAAAARAAMELSRSHDGPSSPNSPGSGTTPHNKQEVEEPKVESKPKP 310
             AA+AA E A QA AAA+ A  L+   D  +   S  S  + H K           P  
Sbjct: 264 ESAAEAATELAKQAVAAAQVASLLATRRDSSNKEFSVSSDHSTHQKDSQYMDHHHHHP-- 323

Query: 311 KMEYGIRREGEGEGEGEEEVKNSVPGASS--PSREFCYGEK--EKSDMETKLEKTELSEK 364
               G RR+     + E     + PGA +    R   Y      +SD E +   TE   K
Sbjct: 324 ----GSRRQSR---DSETSSYYAKPGAENRGMGRRHSYNNPGINESDYEEEYTNTEAEAK 383

BLAST of ClCG08G005190 vs. TAIR 10
Match: AT2G19710.1 (Regulator of Vps4 activity in the MVB pathway protein )

HSP 1 Score: 125.9 bits (315), Expect = 6.6e-29
Identity = 68/175 (38.86%), Postives = 108/175 (61.71%), Query Frame = 0

Query: 9   LGRNFRASKFRPLLSLAVTRLSILTTQRRLRCSQAHSDVLQLLQLPHHHRALLRVEKVIV 68
           L R F+ +K +  L +A +RL IL  ++ ++  Q   ++ QLL+      A +RVE V+ 
Sbjct: 5   LQRGFKPAKCKTALQMANSRLKILKNKKEIQIKQLRRELAQLLESGQTPTARIRVEHVVR 64

Query: 69  DQNALDAYVLIEGYLNLLLERTTLLEQQSECPEELKEAVAGLIFAASRCGDFPELHEIKS 128
           ++  + AY LI  Y  LL+ R  ++E Q  CP +LKEAV  ++FA+ R  D PEL EI  
Sbjct: 65  EEKTVAAYELIGIYCELLVVRLGVIESQKNCPIDLKEAVTSVLFASQRLSDVPELSEIFK 124

Query: 129 VLTNRFGKEFTARAVELRNNCGVNHLLMQKLSTRQPSLESRMEVLKAIASENGIV 184
             T ++GK+F+  AVELR + GV+ LL++KLS + P   +++++L AIA E+ +V
Sbjct: 125 QFTTKYGKDFSTSAVELRPDSGVSRLLVEKLSAKAPDGPTKVKILMAIAEEHNVV 179

BLAST of ClCG08G005190 vs. TAIR 10
Match: AT1G25420.1 (Regulator of Vps4 activity in the MVB pathway protein )

HSP 1 Score: 119.8 bits (299), Expect = 4.7e-27
Identity = 93/314 (29.62%), Postives = 150/314 (47.77%), Query Frame = 0

Query: 5   LDAFLGRNFRASKFRPLLSLAVTRLSILTTQRRLRCSQAHSDVLQLLQLPHHHRALLRVE 64
           L+    R    +K +  L+LA+ R+ +L  +R ++      ++   LQ      A +RVE
Sbjct: 4   LNQLFNRGIFGAKCKTSLNLAIARMKLLQNKRDMQLKHMKKEIAHFLQAGQEPIARIRVE 63

Query: 65  KVIVDQNALDAYVLIEGYLNLLLERTTLLEQQSECPEELKEAVAGLIFAASRCGDFPELH 124
            VI + N   AY ++E +   +L R  +LE + ECP EL+EA+A +IFAA RC + P+L 
Sbjct: 64  HVIREMNLWAAYEILELFCEFILARVPILESEKECPRELREAIASIIFAAPRCSEVPDLL 123

Query: 125 EIKSVLTNRFGKEFTARAVELRNNCGVNHLLMQKLSTRQPSLESRMEVLKAIASE---NG 184
           +IK++   ++GKEF   A ELR + GVN  +++KLS   PS  +R+++LK IA E   N 
Sbjct: 124 QIKNLFGTKYGKEFIMVASELRPDSGVNRTIIEKLSPTSPSGAARLKMLKEIAQEYSLNW 183

Query: 185 IVLQIDQPYPSNEEKLVRNTRQ-----------------NQAEAHSEVK-------EKFQ 244
                +  +  + E L+   +Q                  Q+    EV+       ++FQ
Sbjct: 184 DSSATEAEFMKSHEDLLGGAKQIHRQDGISESRPSQQGYGQSSVSREVESLPAEATQRFQ 243

Query: 245 FFT------------------EVPSGSQQKYKDVADAAQAAFESAAQAAAAARAAMELSR 274
                                + P  +++   DV + A+AA  SA +A AAARAA +L  
Sbjct: 244 KLQAQNPVSKSMPSSKLTSAFQAPPDTRRNQTDVMEIARAALASADRATAAARAAAQLVN 303

BLAST of ClCG08G005190 vs. TAIR 10
Match: AT1G34220.2 (Regulator of Vps4 activity in the MVB pathway protein )

HSP 1 Score: 116.3 bits (290), Expect = 5.2e-26
Identity = 87/299 (29.10%), Postives = 151/299 (50.50%), Query Frame = 0

Query: 5   LDAFLGRNFRASKFRPLLSLAVTRLSILTTQRRLRCSQAHSDVLQLLQLPHHHRALLRVE 64
           LD+F  + F+A+K + LL L + R+ ++  +R  +  Q   ++ +LL+      A +RVE
Sbjct: 4   LDSFFNKGFKAAKCKTLLKLTIPRIKLIRNRREAQIKQMRREIAKLLETGQEATARIRVE 63

Query: 65  KVIVDQNALDAYVLIEGYLNLLLERTTLLEQQSECPEELKEAVAGLIFAASRCGDFPELH 124
            +I ++  + A  ++E +  L+  R  ++E Q ECP +LKEA++ + FAA RC D  EL 
Sbjct: 64  HIIREEKMMAAQEILELFCELIAVRLPIIEAQRECPLDLKEAISSVCFAAPRCSDLTELQ 123

Query: 125 EIKSVLTNRFGKEFTARAVELRNNCGVNHLLMQKLSTRQPSLESRMEVLKAIASENGIVL 184
           +++ +  +++GKEF A A EL+ + GVN  L++ LS R PS E+++++LK IA E+    
Sbjct: 124 QVQILFVSKYGKEFVAAASELKPDSGVNRKLVELLSVRAPSPETKLKLLKEIAEEH---- 183

Query: 185 QIDQPYPSNE-------EKLVRNTRQNQAEAHSEVKEKFQFFTEVPSGSQQKYKDVADAA 244
           ++D    S E       E L+   +Q    +   + E+    T + S S  K K  +D+ 
Sbjct: 184 ELDWDPASTETDLFKSHEDLLDGPKQFGGGSKLPLPEEQNEKTNLTSLSAAKEKSDSDSE 243

Query: 245 QAAFE---------------SAAQAAAAARAAMELSRSHDGPSSPNSPGSGTTPHNKQE 282
               +               ++  A  AA++A     SHD P    + G   T   + E
Sbjct: 244 YDILDFPEVPNVLLRPTPGATSVNAPDAAKSASYEHTSHDLPFDSENAGVEKTASKRDE 298

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
XP_038884116.11.7e-14880.59uncharacterized protein LOC120075040 [Benincasa hispida][more]
XP_011656519.12.1e-13375.81uncharacterized protein LOC101211044 [Cucumis sativus] >KGN45949.1 hypothetical ... [more]
XP_022921602.11.1e-12971.65uncharacterized protein LOC111429814 [Cucurbita moschata] >KAG7032508.1 IST1-lik... [more]
KAG6601794.13.1e-12971.65IST1-like protein, partial [Cucurbita argyrosperma subsp. sororia][more]
XP_023538782.14.4e-12871.39uncharacterized protein LOC111799606 [Cucurbita pepo subsp. pepo][more]
Match NameE-valueIdentityDescription
Q54I397.8e-1129.31IST1-like protein OS=Dictyostelium discoideum OX=44689 GN=DDB_G0289029 PE=3 SV=1[more]
P539906.6e-1030.57IST1 homolog OS=Homo sapiens OX=9606 GN=IST1 PE=1 SV=1[more]
Q9CX006.6e-1030.57IST1 homolog OS=Mus musculus OX=10090 GN=Ist1 PE=1 SV=1[more]
Q568Z66.6e-1030.57IST1 homolog OS=Rattus norvegicus OX=10116 GN=Ist1 PE=2 SV=1[more]
Q3ZBV13.3e-0929.94IST1 homolog OS=Bos taurus OX=9913 GN=IST1 PE=2 SV=1[more]
Match NameE-valueIdentityDescription
A0A0A0KAU09.9e-13475.81Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_6G032470 PE=3 SV=1[more]
A0A6J1E0Z05.1e-13071.65uncharacterized protein LOC111429814 OS=Cucurbita moschata OX=3662 GN=LOC1114298... [more]
A0A6J1I2V35.3e-12770.62uncharacterized protein LOC111470447 OS=Cucurbita maxima OX=3661 GN=LOC111470447... [more]
A0A1S3BD831.1e-12481.15uncharacterized protein LOC103488606 OS=Cucumis melo OX=3656 GN=LOC103488606 PE=... [more]
A0A6J1CFG66.0e-12369.15uncharacterized protein LOC111010325 OS=Momordica charantia OX=3673 GN=LOC111010... [more]
Match NameE-valueIdentityDescription
AT1G13340.12.8e-6449.07Regulator of Vps4 activity in the MVB pathway protein [more]
AT4G35730.12.0e-3030.21Regulator of Vps4 activity in the MVB pathway protein [more]
AT2G19710.16.6e-2938.86Regulator of Vps4 activity in the MVB pathway protein [more]
AT1G25420.14.7e-2729.62Regulator of Vps4 activity in the MVB pathway protein [more]
AT1G34220.25.2e-2629.10Regulator of Vps4 activity in the MVB pathway protein [more]
InterPro
Analysis Name: InterPro Annotations of Watermelon (Charleston Gray) v2.5
Date Performed: 2022-01-31
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR042277Vacuolar protein sorting-associated protein IST1-likeGENE3D1.20.1260.60coord: 6..190
e-value: 1.2E-59
score: 202.9
IPR005061Vacuolar protein sorting-associated protein Ist1PFAMPF03398Ist1coord: 21..182
e-value: 3.7E-48
score: 163.5
IPR005061Vacuolar protein sorting-associated protein Ist1PANTHERPTHR12161IST1 FAMILY MEMBERcoord: 8..368
NoneNo IPR availableMOBIDB_LITEmobidb-litedisorder_predictioncoord: 261..278
NoneNo IPR availableMOBIDB_LITEmobidb-litedisorder_predictioncoord: 252..342
NoneNo IPR availableMOBIDB_LITEmobidb-litedisorder_predictioncoord: 327..342
NoneNo IPR availableMOBIDB_LITEmobidb-litedisorder_predictioncoord: 279..313
NoneNo IPR availablePANTHERPTHR12161:SF16REGULATOR OF VPS4 ACTIVITY IN THE MVB PATHWAY PROTEINcoord: 8..368

Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
ClCG08G005190.1ClCG08G005190.1mRNA


GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
biological_process GO:0015031 protein transport