ClCG08G004150 (gene) Watermelon (Charleston Gray) v2.5

Overview


Name	ClCG08G004150
Type	gene
Organism	Citrullus lanatus subsp. vulgaris cv. Charleston Gray (Watermelon (Charleston Gray) v2.5)
Description	rRNA N-glycosidase
Location	CG_Chr08: 13109017 .. 13112950 (-)
RNA-Seq Expression	ClCG08G004150
Synteny	ClCG08G004150

Sequences

The following sequences are available for this feature:

Gene sequence (with intron)

Legend: polypeptideexonCDS

Hold the cursor over a type above to highlight its positions in the sequence below.

ATGGATGATTCGACTACCATAGAGTTGTACAACTCTAGTACGGTTGGAGGGGTGCCTGCAGAAGGGGAAGATCGAGGATATGGAACCAACAATTTGGATATCAAATTAAGGTACAATCTAGAGTTGTTATTCTGCAAAATGAACATATTGCTTGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAATTGGNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAATCGGGTGAATTCAAGGCTACATCAATCTATTGTGTGACAAATTTTTATTATTATTTTTTATTTTTTTAAAATTGAATTCTTTTTATTTTTTCTATTATATATATAGAAAAGAGATGGGTGGACCTAATCAGTGGCCTATAATACTTTTGTATATCTAGTTTAAAATTTGTATATAAGATTTTCAGGAAATGAGAATAAAAAGGGAAAACAACATAGCGTATAGGTCCTCTAAAAGTGGATTACATTCTATTAATCCAATTAGCTATATTTAAAAAGTGGATTCTCTTTTTTCTTTTGCTTTTCTTTATATTTTGTCCTTTTTTTGTGGGTGGGACTTTAAAAAGTTGACACAGCCTAGCAAATAGCAAGTATTATTTAATTACAAAAATATTAATTTATATCTCTAAACTCTAACTTGTATCCATGAAAACCCTAACTAATAATTGTATAATTTAAACCTTAAATTTTTTCATAAGTGTACCAATTTACCCTCTTCACTAAATTTCATTCTACTAATATTGTATGAAAGAAACTTATAATATAAGTCGATTAATCTCCTAATTTTTCATAAGTCAATCAATTTAGATCTTTCATTATAATTACATTTGAAAATAATCTCTACATTAACCCCTAAACGTTTGTAGACTTCTTCACATGCCGTTTTTATAAAATAGACAATTTGAGTAAATGCATGTATGATTTTCAAAAGAAAATTTGTAAAAGAGTTTAAAGTTTAGAGTTTAATTGATACAATTATAAGTTTCACTTGAGGTTTAAATTGATACACGTTATATTTAGACAACCTCTAAAGTTTAGAATTATGGTTATTTTCAAATATATCAAAATGAGTCAATTTATTTACAAATATATAGAAAAGTGTCACTGTCTATCGGTGATAGACAATAATAAAAAACAATAATCGACTACTATTTCCAACAATTCTGTCATTTAAAACAAGGGTTGTTTTCATATATAGGAAAATAAACCAAAATATTTACAAATATAGCAAACTGGCTATCTGCGATAAACTGCGATAGATTGTGACATATTTTGCTATTATTTAAAAATATTTTCAACAATTTTAGTCATTTAAAATAATTTATCTTAAAACAATTACACTTGCAATTATATATATATATATAAATTAATATTTTCCCCAATTTAATTTTATTTAATGCGAGATCCATTTGAGTCTCTTGTTTAACTTGTATATAAATATAGAGAAAATGGTCTTAGATTGTGTACTTTGAGTATTGATGTTCAAGAATTATATAAACATGGAGTTGATTCAATCCTTTCCCTTAAATTACAGTGCTCGTGCTGCCAACCATGCCGATCGTCGACCTGCCGATTCGAATCTCAGCTTCTCTCTGGTTGGTGCCACTGCTTCTACTTACAAACAATTCATATCAGATCTACGTAAGGCACTTACACTAAACTCCCACACAGTCTACAACATACCTGTCTTAGCATCCACAGCCAAAGGGCTCAACCGCTTCATATTGCTCAATCTCACCAATTACCAAGCCGAATCCATCACTCTCGCAATTGACGTAGTTGATGTTTACGTAGTTGCATACCAAGCCCAAAACAAAGCCTATTTCCTCAAGAACGTTTCCAAGGAAGCACATGATGTGTTATTTAAAGGTATGAAACATGAAACACTTCCTTACAAAGGGAATTACGGTGACCTGGAGAGATTTGCTGGAAAGATTTCGAGAGAGAATATTGATCTTGGATTATCTGAACTAGGGAGGGCAATTGGAAATCTGTTCCATTATAAGGCTGGGAATTCTGTGCCAAGAGCATTCATTGTGATCATTCAACGATTTCAGAAGCTCCAAGATTTAAGTATATTGAGTCACAGTATGTTAGGTCTAAGTTTAAACCTGACCCTGGATTCATAAGCTTAGAAAATCGTTGGTCCGATCTCTCGGAACAGATACAGAAGGCGGAAAAGCGAGGAGGGAAATTTAAGGCTCCAGTTCCGGTTCGAAGTGTTAGCAATAAAGTAATTATGGTAAGTGATGTTAATTCGCCAGTCGTTAAAGGGATTGCGCTATTACTTCCCGATAAGCTGGCAGAAGCAAAAAATGGAAATCTGAAACTGATGATGGAAGAGGTTAATCAACGAGGTGATTTTGGAATTGATTTCTGA

mRNA sequence

ATGGATGATTCGACTACCATAGAGTTGTACAACTCTAGTACGGTTGGAGGGGTGCCTGCAGAAGGGGAAGATCGAGGATATGGAACCAACAATTTGGATATCAAATTAAGTGCTCGTGCTGCCAACCATGCCGATCGTCGACCTGCCGATTCGAATCTCAGCTTCTCTCTGGTTGGTGCCACTGCTTCTACTTACAAACAATTCATATCAGATCTACGTAAGGCACTTACACTAAACTCCCACACAGTCTACAACATACCTGTCTTAGCATCCACAGCCAAAGGGCTCAACCGCTTCATATTGCTCAATCTCACCAATTACCAAGCCGAATCCATCACTCTCGCAATTGACGTAGTTGATGTTTACGTAGTTGCATACCAAGCCCAAAACAAAGCCTATTTCCTCAAGAACGTTTCCAAGGAAGCACATGATGTGTTATTTAAAGGCTGGGAATTCTGTGCCAAGAGCATTCATTGTGATCATTCAACGATTTCAGAAGCTCCAAGATTTAAGTATATTGAGTCACAGTATGTTAGGTCTAAGTTTAAACCTGACCCTGGATTCATAAGCTTAGAAAATCGTTGGTCCGATCTCTCGGAACAGATACAGAAGGCGGAAAAGCGAGGAGGGAAATTTAAGGCTCCAGTTCCGGTTCGAAGTGTTAGCAATAAAGTAATTATGGTAAGTGATGTTAATTCGCCAGTCGTTAAAGGGATTGCGCTATTACTTCCCGATAAGCTGGCAGAAGCAAAAAATGGAAATCTGAAACTGATGATGGAAGAGGTTAATCAACGAGGTGATTTTGGAATTGATTTCTGA

Coding sequence (CDS)

ATGGATGATTCGACTACCATAGAGTTGTACAACTCTAGTACGGTTGGAGGGGTGCCTGCAGAAGGGGAAGATCGAGGATATGGAACCAACAATTTGGATATCAAATTAAGTGCTCGTGCTGCCAACCATGCCGATCGTCGACCTGCCGATTCGAATCTCAGCTTCTCTCTGGTTGGTGCCACTGCTTCTACTTACAAACAATTCATATCAGATCTACGTAAGGCACTTACACTAAACTCCCACACAGTCTACAACATACCTGTCTTAGCATCCACAGCCAAAGGGCTCAACCGCTTCATATTGCTCAATCTCACCAATTACCAAGCCGAATCCATCACTCTCGCAATTGACGTAGTTGATGTTTACGTAGTTGCATACCAAGCCCAAAACAAAGCCTATTTCCTCAAGAACGTTTCCAAGGAAGCACATGATGTGTTATTTAAAGGCTGGGAATTCTGTGCCAAGAGCATTCATTGTGATCATTCAACGATTTCAGAAGCTCCAAGATTTAAGTATATTGAGTCACAGTATGTTAGGTCTAAGTTTAAACCTGACCCTGGATTCATAAGCTTAGAAAATCGTTGGTCCGATCTCTCGGAACAGATACAGAAGGCGGAAAAGCGAGGAGGGAAATTTAAGGCTCCAGTTCCGGTTCGAAGTGTTAGCAATAAAGTAATTATGGTAAGTGATGTTAATTCGCCAGTCGTTAAAGGGATTGCGCTATTACTTCCCGATAAGCTGGCAGAAGCAAAAAATGGAAATCTGAAACTGATGATGGAAGAGGTTAATCAACGAGGTGATTTTGGAATTGATTTCTGA

Protein sequence

MDDSTTIELYNSSTVGGVPAEGEDRGYGTNNLDIKLSARAANHADRRPADSNLSFSLVGATASTYKQFISDLRKALTLNSHTVYNIPVLASTAKGLNRFILLNLTNYQAESITLAIDVVDVYVVAYQAQNKAYFLKNVSKEAHDVLFKGWEFCAKSIHCDHSTISEAPRFKYIESQYVRSKFKPDPGFISLENRWSDLSEQIQKAEKRGGKFKAPVPVRSVSNKVIMVSDVNSPVVKGIALLLPDKLAEAKNGNLKLMMEEVNQRGDFGIDF

Homology

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BLAST of ClCG08G004150 vs. NCBI nr
Match: XP_022926255.1 (ribosome-inactivating protein bryodin II-like [Cucurbita moschata])

HSP 1 Score: 276.9 bits (707), Expect = 1.8e-70
Identity = 164/291 (56.36%), Postives = 194/291 (66.67%), Query Frame = 0


Query: 34  IKLSARAANHADRRPADSNLSFSLVGATASTYKQFISDLRKALTLNSHTVYNIPVLASTA 93
           +   A AA  A R  A++NLSFSL+GATA TYKQFI+DLRKALT+NSH VY+IPVL +TA
Sbjct: 17  LNCGAHAAIRAPRPSANANLSFSLLGATAQTYKQFINDLRKALTVNSHKVYDIPVLPATA 76

Query: 94  KGLNRFILLNLTNYQAESITLAIDVVDVYVVAYQAQNKAYFLKNVSKEAHDVLFKGWEFC 153
           KGL RFIL+NLTNY+ ESIT+AID VDVY+VAYQA NKAYFLK+VSKEAHDVLFKG +  
Sbjct: 77  KGLARFILVNLTNYKGESITVAIDTVDVYIVAYQAGNKAYFLKDVSKEAHDVLFKGSKHE 136

Query: 154 AKSIHCDH----------------------------------------------STISEA 213
             S   ++                                                +SEA
Sbjct: 137 TLSYKGNYDDLERHAGKISRENIELGFSELSSSIGNMFHYKAGYSVPRSFIVMIQMLSEA 196

Query: 214 PRFKYIES---QYVRSKFKPDPGFISLENRWSDLSEQIQKAEKRGGKFKAPVPVRSVSNK 273
            RFKYIE+   + V SKFKPDPG++SLENRWS+LSEQ+QKA+K GGKF+ PV VRSVSNK
Sbjct: 197 SRFKYIETRNCEKVESKFKPDPGYLSLENRWSNLSEQVQKAQKHGGKFEHPVQVRSVSNK 256

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BLAST of ClCG08G004150 vs. NCBI nr
Match: XP_022981562.1 (ribosome-inactivating protein bryodin II-like [Cucurbita maxima])

HSP 1 Score: 274.6 bits (701), Expect = 9.0e-70
Identity = 164/291 (56.36%), Postives = 191/291 (65.64%), Query Frame = 0


Query: 34  IKLSARAANHADRRPADSNLSFSLVGATASTYKQFISDLRKALTLNSHTVYNIPVLASTA 93
           +   A  A  A R  A++NLSFSL+GATA TYKQFISDLRKALT+NSH VY+IPVL +TA
Sbjct: 17  LNCGAHVAIRAPRPSANANLSFSLLGATAQTYKQFISDLRKALTVNSHKVYDIPVLPATA 76

Query: 94  KGLNRFILLNLTNYQAESITLAIDVVDVYVVAYQAQNKAYFLKNVSKEAHDVLFKGWEF- 153
           KGL RFIL+NLTNY+ ESIT+AID VDVY+VAYQA NKAYFLK+VSKEAHDVLFKG +  
Sbjct: 77  KGLARFILVNLTNYKGESITVAIDTVDVYIVAYQAGNKAYFLKDVSKEAHDVLFKGSKHE 136

Query: 154 ---------------------------------------------CAKSIHCDHSTISEA 213
                                                          KS       +SEA
Sbjct: 137 TLSYKGNYDDLERHAGKISRENIDLGFSELSSSIGNMFHYNAGYSVPKSFIVMIQMLSEA 196

Query: 214 PRFKYIES---QYVRSKFKPDPGFISLENRWSDLSEQIQKAEKRGGKFKAPVPVRSVSNK 273
            RFKYIE+   + V SKFKPDPG++SLENRWS+LSEQ+QKA+K GGKF+ PV VRSVSNK
Sbjct: 197 SRFKYIEARNCENVESKFKPDPGYLSLENRWSNLSEQVQKAQKHGGKFEHPVQVRSVSNK 256

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BLAST of ClCG08G004150 vs. NCBI nr
Match: KAG7037232.1 (hypothetical protein SDJN02_00855, partial [Cucurbita argyrosperma subsp. argyrosperma])

HSP 1 Score: 273.5 bits (698), Expect = 2.0e-69
Identity = 163/291 (56.01%), Postives = 193/291 (66.32%), Query Frame = 0


Query: 34  IKLSARAANHADRRPADSNLSFSLVGATASTYKQFISDLRKALTLNSHTVYNIPVLASTA 93
           +   A AA  A R  A++NLSFSL+GATA TYKQFI+DLRKALT+NSH VY+IPVL +TA
Sbjct: 17  LNCGAHAAIRAPRPSANANLSFSLLGATAQTYKQFINDLRKALTVNSHKVYDIPVLPATA 76

Query: 94  KGLNRFILLNLTNYQAESITLAIDVVDVYVVAYQAQNKAYFLKNVSKEAHDVLFKGWEFC 153
           KGL RFIL+NLTNY+ ESIT+AID VDVY+VAYQA NKAYFLK+VSKEAHDVLFKG +  
Sbjct: 77  KGLARFILVNLTNYKGESITVAIDTVDVYIVAYQAGNKAYFLKDVSKEAHDVLFKGSKHE 136

Query: 154 AKSIHCDH----------------------------------------------STISEA 213
             S   ++                                                +SEA
Sbjct: 137 TLSYKGNYDDLERHAGKISRENIELGFSELSSSIGNMFHYNAGYSVPRSFIVMIQMLSEA 196

Query: 214 PRFKYIES---QYVRSKFKPDPGFISLENRWSDLSEQIQKAEKRGGKFKAPVPVRSVSNK 273
            RFKYIE+   + V SKFKPDPG++SLENRWS+LSEQ+QKA+K GGKF+ PV VRSVSNK
Sbjct: 197 SRFKYIETRNCEKVESKFKPDPGYLSLENRWSNLSEQVQKAQKHGGKFEHPVQVRSVSNK 256

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BLAST of ClCG08G004150 vs. NCBI nr
Match: XP_023523582.1 (ribosome-inactivating protein bryodin II-like [Cucurbita pepo subsp. pepo])

HSP 1 Score: 268.5 bits (685), Expect = 6.4e-68
Identity = 161/291 (55.33%), Postives = 190/291 (65.29%), Query Frame = 0


Query: 34  IKLSARAANHADRRPADSNLSFSLVGATASTYKQFISDLRKALTLNSHTVYNIPVLASTA 93
           +   A AA  A R  A++NLSFSL+GATA TYKQFI+DLR ALT+ SH VY IPVL +TA
Sbjct: 17  LNCGAHAAIRAPRPSANANLSFSLLGATAQTYKQFINDLRNALTVQSHKVYGIPVLPATA 76

Query: 94  KGLNRFILLNLTNYQAESITLAIDVVDVYVVAYQAQNKAYFLKNVSKEAHDVLFKGWEFC 153
           KGL RFIL+NLTNY+ ESIT+AID VDVY+VAYQA NKAYFLK+VSKEAHDVLFKG +  
Sbjct: 77  KGLARFILVNLTNYKGESITVAIDTVDVYIVAYQAGNKAYFLKDVSKEAHDVLFKGSKHE 136

Query: 154 AKSIHCDH----------------------------------------------STISEA 213
             S   ++                                                +SEA
Sbjct: 137 TLSYKGNYDDLERHAGMISRENIELGFSELSSSIGNMFHYNAGYSVPRSFIVMIQMLSEA 196

Query: 214 PRFKYIES---QYVRSKFKPDPGFISLENRWSDLSEQIQKAEKRGGKFKAPVPVRSVSNK 273
            RFKYIE+   + V SKFKPDPG++SLENRWS+LSEQ+QKA+K GGKF+ PV VRSVSNK
Sbjct: 197 SRFKYIETRNCEKVESKFKPDPGYLSLENRWSNLSEQVQKAQKHGGKFERPVQVRSVSNK 256

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BLAST of ClCG08G004150 vs. NCBI nr
Match: KAG7037234.1 (hypothetical protein SDJN02_00857, partial [Cucurbita argyrosperma subsp. argyrosperma])

HSP 1 Score: 263.8 bits (673), Expect = 1.6e-66
Identity = 160/291 (54.98%), Postives = 188/291 (64.60%), Query Frame = 0


Query: 34  IKLSARAANHADRRPADSNLSFSLVGATASTYKQFISDLRKALTLNSHTVYNIPVLASTA 93
           +   A AA    R  A++N+SFSL+GATA TYKQFI DLRKALT+NSH VY IPVL +TA
Sbjct: 17  LNCGAHAAIRVPRPSANANVSFSLLGATAETYKQFIKDLRKALTVNSHKVYGIPVLPATA 76

Query: 94  KGLNRFILLNLTNYQAESITLAIDVVDVYVVAYQAQNKAYFLKNVSKEAHDVLFKG---- 153
           KGL RFIL+NLTNY  ESIT+AID VDVY+VAYQA NKAYFLK+VSKEAH+VLFKG    
Sbjct: 77  KGLARFILVNLTNYNGESITVAIDTVDVYIVAYQAGNKAYFLKDVSKEAHNVLFKGSKHE 136

Query: 154 --W-------------EFCAKSIHCDHS---------------------------TISEA 213
             W             +   +++    S                            +SEA
Sbjct: 137 TLWYKGNYDDLERHAGKISRENVQLGFSELSSSIGNMFHYNAGYSVPRSFIVMIQMLSEA 196

Query: 214 PRFKYIE---SQYVRSKFKPDPGFISLENRWSDLSEQIQKAEKRGGKFKAPVPVRSVSNK 273
            RFKYIE   S  V S FKPDPG++SLENRW++LSEQ+QKA+K GGKF+  V VRSV NK
Sbjct: 197 SRFKYIEKINSDNVSSTFKPDPGYLSLENRWNNLSEQVQKAQKHGGKFEHAVQVRSVGNK 256

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BLAST of ClCG08G004150 vs. ExPASy Swiss-Prot
Match: P98184 (Ribosome-inactivating protein bryodin II OS=Bryonia dioica OX=3652 PE=1 SV=1)

HSP 1 Score: 188.0 bits (476), Expect = 1.4e-46
Identity = 115/243 (47.33%), Postives = 147/243 (60.49%), Query Frame = 0


Query: 50  DSNLSFSLVGATASTYKQFISDLRKALTLNSHTVYNIPVLASTAKGLNRFILLNLTNYQA 109
           D +++FSL+GAT +TYK FI +LR  LT+ +  VY+IPVL + A GL RF L+ LTNY  
Sbjct: 21  DVDINFSLIGATGATYKTFIRNLRTKLTVGTPRVYDIPVLRNAAAGLARFQLVTLTNYNG 80

Query: 110 ESITLAIDVVDVYVVAYQAQNKAYFLKNVSKEAHDVLF--------------KGWEFCAK 169
           ES+T+A+DVV+VYVVAY+A N AYFL + S EA++VLF               G E  A 
Sbjct: 81  ESVTVALDVVNVYVVAYRAGNTAYFLADASTEANNVLFAGINHVRLPYGGNYDGLETAAG 140

Query: 170 SIHCDH--------------------------------STISEAPRFKYIE---SQYVRS 229
            I  ++                                 T+SEA RFKYIE   S+ V +
Sbjct: 141 RISRENIELGFSEISSAIGNMFRHNPGTSVPRAFIVIIQTVSEAARFKYIEQRVSENVGT 200

Query: 230 KFKPDPGFISLENRWSDLSEQIQKAEKRGGKFKAPVPVRSVSNKVIMVSDVNSPVVKGIA 244
           KFKPDP F+SL+N W  LSEQIQ A+ RGG+F  PV +R+VSN    V++VNSPVVKGIA
Sbjct: 201 KFKPDPAFLSLQNAWGSLSEQIQIAQTRGGEFARPVELRTVSNTPTFVTNVNSPVVKGIA 260

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BLAST of ClCG08G004150 vs. ExPASy Swiss-Prot
Match: P24817 (Ribosome-inactivating protein beta-momorcharin OS=Momordica charantia OX=3673 GN=MAP30 PE=1 SV=2)

HSP 1 Score: 142.9 bits (359), Expect = 5.3e-33
Identity = 99/258 (38.37%), Postives = 138/258 (53.49%), Query Frame = 0


Query: 49  ADSNLSFSLVGATASTYKQFISDLRKALTLNSHTVYNIPVLASTAKGLNRFILLNLTNYQ 108
           A  +++F L  ATA TY +FI D R  L   SH VY+IP+L ST     RFILLNLT+Y 
Sbjct: 21  AKGDVNFDLSTATAKTYTKFIEDFRATLPF-SHKVYDIPLLYSTISDSRRFILLNLTSYA 80

Query: 109 AESITLAIDVVDVYVVAYQAQNKAYFLKNVSKEAHDVLFKG-----------WEFCAKSI 168
            E+I++AIDV +VYVVAY+ ++ +YF K    EA+++LFKG           +E    + 
Sbjct: 81  YETISVAIDVTNVYVVAYRTRDVSYFFKESPPEAYNILFKGTRKITLPYTGNYENLQTAA 140

Query: 169 HCDH---------------------------------STISEAPRFKYIE---SQYVRSK 228
           H                                     T +EA RFKYIE   ++YV + 
Sbjct: 141 HKIRENIDLGLPALSSAITTLFYYNAQSAPSALLVLIQTTAEAARFKYIERHVAKYVATN 200

Query: 229 FKPDPGFISLENRWSDLSEQIQKAEKRGGKFKAPVPVRSVSNKVIMVSDVNSPVVKG-IA 259
           FKP+   ISLEN+WS LS+QI  A+ +GGKF+ PV +   + +   V++V+S VVKG I 
Sbjct: 201 FKPNLAIISLENQWSALSKQIFLAQNQGGKFRNPVDLIKPTGERFQVTNVDSDVVKGNIK 260

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BLAST of ClCG08G004150 vs. ExPASy Swiss-Prot
Match: P29339 (Ribosome-inactivating protein momordin II OS=Momordica balsamina OX=3672 PE=3 SV=1)

HSP 1 Score: 141.0 bits (354), Expect = 2.0e-32
Identity = 98/258 (37.98%), Postives = 138/258 (53.49%), Query Frame = 0


Query: 49  ADSNLSFSLVGATASTYKQFISDLRKALTLNSHTVYNIPVLASTAKGLNRFILLNLTNYQ 108
           A  +++F L  ATA TY +FI D R  L   SH VY+IP+L ST     RFILL+LT+Y 
Sbjct: 21  AKGDVNFDLSTATAKTYTKFIEDFRATLPF-SHKVYDIPLLYSTISDSRRFILLDLTSYA 80

Query: 109 AESITLAIDVVDVYVVAYQAQNKAYFLKNVSKEAHDVLFKG-----------WEFCAKSI 168
            E+I++AIDV +VYVVAY+ ++ +YF K    EA+++LFKG           +E    + 
Sbjct: 81  YETISVAIDVTNVYVVAYRTRDVSYFFKESPPEAYNILFKGTRKITLPYTGNYENLQTAA 140

Query: 169 HCDH---------------------------------STISEAPRFKYIE---SQYVRSK 228
           H                                     T +EA RFKYIE   ++YV + 
Sbjct: 141 HKIRENIDLGLPALSSAITTLFYYNAQSAPSALLVLIQTTAEAARFKYIERHVAKYVATN 200

Query: 229 FKPDPGFISLENRWSDLSEQIQKAEKRGGKFKAPVPVRSVSNKVIMVSDVNSPVVKG-IA 259
           FKP+   ISLEN+WS LS+QI  A+ +GGKF+ PV +   + +   V++V+S VVKG I 
Sbjct: 201 FKPNLAIISLENQWSALSKQIFLAQNQGGKFRNPVDLIKPTGERFQVTNVDSDVVKGNIK 260

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BLAST of ClCG08G004150 vs. ExPASy Swiss-Prot
Match: P84531 (Ribosome-inactivating protein cucurmosin OS=Cucurbita moschata OX=3662 PE=1 SV=2)

HSP 1 Score: 128.6 bits (322), Expect = 1.0e-28
Identity = 91/240 (37.92%), Postives = 123/240 (51.25%), Query Frame = 0


Query: 52  NLSFSLVGATASTYKQFISDLRKALTLNSHTVYNIPVLASTAKGLNRFILLNLTNYQAES 111
           N+ F L  AT+S+YK FI +LR+AL  +   VY+IPVL ST     RFIL++L NY  +S
Sbjct: 1   NVRFDLSSATSSSYKTFIKNLREALPKDG-KVYDIPVLLSTVMDSRRFILIDLVNYDGQS 60

Query: 112 ITLAIDVVDVYVVAYQAQNKAYFLKNVSKEAHDVLFKGW--------------------- 171
           IT AIDV++VY+VAY     +YF + V  +A  +LFKG                      
Sbjct: 61  ITAAIDVLNVYIVAYSTGTVSYFFQQVPAQAPKLLFKGTQQRTLPYTGNYENLQTAAKKL 120

Query: 172 -----------------------EFCAKSIHCDHSTISEAPRFKYIESQY---VRSKFKP 231
                                  E  A ++     T SEA RF+YIE Q    V +KFKP
Sbjct: 121 RENIELGLPALDSAITTLFHYNAEAAASALLVLIQTTSEAARFRYIELQIANNVGTKFKP 180

Query: 232 DPGFISLENRWSDLSEQIQKAEKRGGKFKAPVPVRSVSNKVIMVSDVNSPVV-KGIALLL 244
               ISLEN WS LS+QIQ A+ + G+F+ PV +       + +++V S VV + I LLL
Sbjct: 181 SQTIISLENNWSALSKQIQIAKNKNGQFETPVILIDPQGNRVQITNVTSNVVTQNIQLLL 239

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BLAST of ClCG08G004150 vs. ExPASy Swiss-Prot
Match: Q00465 (Ribosome-inactivating protein luffin-alpha OS=Luffa aegyptiaca OX=3670 PE=1 SV=1)

HSP 1 Score: 117.9 bits (294), Expect = 1.8e-25
Identity = 86/243 (35.39%), Postives = 127/243 (52.26%), Query Frame = 0


Query: 50  DSNLSFSLVGATASTYKQFISDLRKALTLNSHTVYNIPVLASTAKGLNRFILLNLTNYQA 109
           ++++ FSL G+++++Y +FI DLRKAL  N  TVYNI +L S+A G +R+ L+ L+NY  
Sbjct: 19  EADVRFSLSGSSSTSYSKFIGDLRKALPSNG-TVYNITLLLSSASGASRYTLMTLSNYDG 78

Query: 110 ESITLAIDVVDVYVVAYQAQNKAYFL-KNVSKEAHDVLFKG--------------WEFCA 169
           ++IT+A+DV +VY++ Y   + +YF  ++ +K A   +FKG               +  A
Sbjct: 79  KAITVAVDVTNVYIMGYLVNSTSYFFNESDAKLASQYVFKGSTIVTLPYSGNYEKLQTAA 138

Query: 170 KSI-------------------HCDHS-----------TISEAPRFKYIESQYVRSKFK- 229
             I                   H D +           T +EA RFKYIE Q +    K 
Sbjct: 139 GKIREKIPLGFPALDSAITTLFHYDSTAAAAAFLVIIQTTAEASRFKYIEGQIIERISKN 198

Query: 230 --PDPGFISLENRWSDLSEQIQKAEKRGGKFKAPVPVRSVSNKVIMVSDVNSPVV-KGIA 244
             P    ISLEN WS LS+QIQ A+   G FK PV +     + + +++V S VV K I 
Sbjct: 199 QVPSLATISLENEWSALSKQIQLAQTNNGTFKTPVVITDDKGQRVEITNVTSKVVTKNIQ 258

[Close]

BLAST of ClCG08G004150 vs. ExPASy TrEMBL
Match: A0A6J1EKL0 (rRNA N-glycosidase OS=Cucurbita moschata OX=3662 GN=LOC111433434 PE=3 SV=1)

HSP 1 Score: 276.9 bits (707), Expect = 8.8e-71
Identity = 164/291 (56.36%), Postives = 194/291 (66.67%), Query Frame = 0


Query: 34  IKLSARAANHADRRPADSNLSFSLVGATASTYKQFISDLRKALTLNSHTVYNIPVLASTA 93
           +   A AA  A R  A++NLSFSL+GATA TYKQFI+DLRKALT+NSH VY+IPVL +TA
Sbjct: 17  LNCGAHAAIRAPRPSANANLSFSLLGATAQTYKQFINDLRKALTVNSHKVYDIPVLPATA 76

Query: 94  KGLNRFILLNLTNYQAESITLAIDVVDVYVVAYQAQNKAYFLKNVSKEAHDVLFKGWEFC 153
           KGL RFIL+NLTNY+ ESIT+AID VDVY+VAYQA NKAYFLK+VSKEAHDVLFKG +  
Sbjct: 77  KGLARFILVNLTNYKGESITVAIDTVDVYIVAYQAGNKAYFLKDVSKEAHDVLFKGSKHE 136

Query: 154 AKSIHCDH----------------------------------------------STISEA 213
             S   ++                                                +SEA
Sbjct: 137 TLSYKGNYDDLERHAGKISRENIELGFSELSSSIGNMFHYKAGYSVPRSFIVMIQMLSEA 196

Query: 214 PRFKYIES---QYVRSKFKPDPGFISLENRWSDLSEQIQKAEKRGGKFKAPVPVRSVSNK 273
            RFKYIE+   + V SKFKPDPG++SLENRWS+LSEQ+QKA+K GGKF+ PV VRSVSNK
Sbjct: 197 SRFKYIETRNCEKVESKFKPDPGYLSLENRWSNLSEQVQKAQKHGGKFEHPVQVRSVSNK 256

[Close]

BLAST of ClCG08G004150 vs. ExPASy TrEMBL
Match: A0A6J1IUB2 (rRNA N-glycosidase OS=Cucurbita maxima OX=3661 GN=LOC111480641 PE=3 SV=1)

HSP 1 Score: 274.6 bits (701), Expect = 4.3e-70
Identity = 164/291 (56.36%), Postives = 191/291 (65.64%), Query Frame = 0


Query: 34  IKLSARAANHADRRPADSNLSFSLVGATASTYKQFISDLRKALTLNSHTVYNIPVLASTA 93
           +   A  A  A R  A++NLSFSL+GATA TYKQFISDLRKALT+NSH VY+IPVL +TA
Sbjct: 17  LNCGAHVAIRAPRPSANANLSFSLLGATAQTYKQFISDLRKALTVNSHKVYDIPVLPATA 76

Query: 94  KGLNRFILLNLTNYQAESITLAIDVVDVYVVAYQAQNKAYFLKNVSKEAHDVLFKGWEF- 153
           KGL RFIL+NLTNY+ ESIT+AID VDVY+VAYQA NKAYFLK+VSKEAHDVLFKG +  
Sbjct: 77  KGLARFILVNLTNYKGESITVAIDTVDVYIVAYQAGNKAYFLKDVSKEAHDVLFKGSKHE 136

Query: 154 ---------------------------------------------CAKSIHCDHSTISEA 213
                                                          KS       +SEA
Sbjct: 137 TLSYKGNYDDLERHAGKISRENIDLGFSELSSSIGNMFHYNAGYSVPKSFIVMIQMLSEA 196

Query: 214 PRFKYIES---QYVRSKFKPDPGFISLENRWSDLSEQIQKAEKRGGKFKAPVPVRSVSNK 273
            RFKYIE+   + V SKFKPDPG++SLENRWS+LSEQ+QKA+K GGKF+ PV VRSVSNK
Sbjct: 197 SRFKYIEARNCENVESKFKPDPGYLSLENRWSNLSEQVQKAQKHGGKFEHPVQVRSVSNK 256

[Close]

BLAST of ClCG08G004150 vs. ExPASy TrEMBL
Match: A0A6J1E093 (rRNA N-glycosidase OS=Momordica charantia OX=3673 GN=LOC111024726 PE=3 SV=1)

HSP 1 Score: 240.0 bits (611), Expect = 1.2e-59
Identity = 152/294 (51.70%), Postives = 185/294 (62.93%), Query Frame = 0


Query: 33  DIKLSARAANHADRRPADSNLSFSLVGATASTYKQFISDLRKALTLNSHTVYNIPVLAST 92
           DI +    AN A   P +SN+SFSL+GAT  TY+QFI +LR ALT+NS  VYNIPVLA+T
Sbjct: 18  DIGIHGVHANGAVNAP-NSNVSFSLLGATGKTYQQFIQNLRNALTINSKIVYNIPVLAAT 77

Query: 93  AKGLNRFILLNLTNYQAESITLAIDVVDVYVVAYQAQNKAYFLKNVSKEAHDVLFK---- 152
           A    RFIL++LTNY+ E+IT+AIDVV+VY+VAYQA NKAYFLK+ SKEA DVLFK    
Sbjct: 78  ALSSARFILVHLTNYKNETITVAIDVVNVYIVAYQAGNKAYFLKDASKEARDVLFKGIKQ 137

Query: 153 ----------GWEFCAKSIHCDH--------------------------------STISE 212
                     G E  A  I  +                                  TISE
Sbjct: 138 EILPYKGNYDGLETAAGKISREKIDLGFSELGSAIGNMYHYNAGTSVPRAFIVMIQTISE 197

Query: 213 APRFKYIE---SQYVRSKFKPDPGFISLENRWSDLSEQIQKAEKRGGKFKAPVPVRSVSN 272
           A RFKYIE   SQ V +KFKPDPGF+SLENRWSDLSEQ+Q A+ R G+F  P+ VRSV+N
Sbjct: 198 AARFKYIETKVSQNVETKFKPDPGFLSLENRWSDLSEQVQIAQNRKGEFARPIEVRSVTN 257

[Close]

BLAST of ClCG08G004150 vs. ExPASy TrEMBL
Match: A0A6J1DVE7 (rRNA N-glycosidase OS=Momordica charantia OX=3673 GN=LOC111024729 PE=3 SV=1)

HSP 1 Score: 206.8 bits (525), Expect = 1.1e-49
Identity = 125/257 (48.64%), Postives = 165/257 (64.20%), Query Frame = 0


Query: 47  RPADSNLSFSLVGATASTYKQFISDLRKALTLNSHTVYNIPVLASTAKGLNRFILLNLTN 106
           R A++++SFS++G+T+ TYKQFI ++R ALT+ S  VY+IPVL +TA G  RF+L++LTN
Sbjct: 17  RAAEADVSFSMLGSTSETYKQFIKNVRSALTVGSQIVYDIPVLKATATGSARFLLVHLTN 76

Query: 107 YQAESITLAIDVVDVYVVAYQAQNKAYFLKNVSKEAHDVLFK--------------GWEF 166
           Y +ESI +AIDVV+VYVVAY+A N AYFL + S EA  VLF+              G E 
Sbjct: 77  YNSESIIVAIDVVNVYVVAYRAGNNAYFLNDASAEADRVLFQGINHVKLSYKGNYDGLET 136

Query: 167 CAK-------------------SIHCDHST------------ISEAPRFKYIE---SQYV 226
            AK                     H +  T            +SEA RFKYIE   S+ V
Sbjct: 137 AAKISREKIDLGFSEISSSIGNMFHHNAGTSVPRAFIVIIQSVSEAARFKYIEQRVSENV 196

Query: 227 RSKFKPDPGFISLENRWSDLSEQIQKAEKRGGKFKAPVPVRSVSNKVIMVSDVNSPVVKG 256
           ++ FKPDP F+SL+NRWSDLSEQIQ A+KRGG+F   V +R+VSNK I+V+DV+SPVVKG
Sbjct: 197 KTTFKPDPAFLSLQNRWSDLSEQIQIAQKRGGEFARAVELRTVSNKPILVTDVSSPVVKG 256

[Close]

BLAST of ClCG08G004150 vs. ExPASy TrEMBL
Match: A0A6J1DWK4 (rRNA N-glycosidase OS=Momordica charantia OX=3673 GN=LOC111024728 PE=3 SV=1)

HSP 1 Score: 204.1 bits (518), Expect = 7.2e-49
Identity = 123/258 (47.67%), Postives = 163/258 (63.18%), Query Frame = 0


Query: 49  ADSNLSFSLVGATASTYKQFISDLRKALTLNSHTVYNIPVLASTAKGLNRFILLNLTNYQ 108
           A++++SFS++GAT+ TY+QFI +LR  LT+ S  VY IPVL STA G  RFIL++LTNY 
Sbjct: 19  AEADVSFSMLGATSETYRQFIRNLRSTLTIRSPVVYGIPVLQSTAVGSARFILVHLTNYN 78

Query: 109 AESITLAIDVVDVYVVAYQAQNKAYFLKNVSKEAHDVLFKG------------------- 168
            ESIT+AIDVV++YVVAY+A N AYFL + S EA++VLFKG                   
Sbjct: 79  TESITVAIDVVNIYVVAYRAGNNAYFLNDASAEANNVLFKGIKHVRLPYKGNYDGLETAA 138

Query: 169 WEFCAKSIHCDHSTIS---------------------------EAPRFKYIE---SQYVR 228
            +   + I    S IS                           EA RFKYIE   S+ V+
Sbjct: 139 GKISREKIDLGFSEISSSIGNMFHHNVGTSIARAFIVIIQSVLEAARFKYIEQRVSENVK 198

Query: 229 SKFKPDPGFISLENRWSDLSEQIQKAEKRGGKFKAPVPVRSVSNKVIMVSDVNSPVVKGI 258
           +KFKPDP F+SLENRWSDLSEQIQ A+KR G+F   + +R+VSNK I+V++V+SP+VKGI
Sbjct: 199 TKFKPDPAFLSLENRWSDLSEQIQIAQKREGEFARAIELRTVSNKPIIVTNVSSPIVKGI 258

The following BLAST results are available for this feature:

BLAST of ClCG08G004150 vs. NCBI nr


Match Name	E-value	Identity	Description
XP_022926255.1	1.8e-70	56.36	ribosome-inactivating protein bryodin II-like [Cucurbita moschata]	[more]
XP_022981562.1	9.0e-70	56.36	ribosome-inactivating protein bryodin II-like [Cucurbita maxima]	[more]
KAG7037232.1	2.0e-69	56.01	hypothetical protein SDJN02_00855, partial [Cucurbita argyrosperma subsp. argyro...	[more]
XP_023523582.1	6.4e-68	55.33	ribosome-inactivating protein bryodin II-like [Cucurbita pepo subsp. pepo]	[more]
KAG7037234.1	1.6e-66	54.98	hypothetical protein SDJN02_00857, partial [Cucurbita argyrosperma subsp. argyro...	[more]

BLAST of ClCG08G004150 vs. ExPASy Swiss-Prot


Match Name	E-value	Identity	Description
P98184	1.4e-46	47.33	Ribosome-inactivating protein bryodin II OS=Bryonia dioica OX=3652 PE=1 SV=1	[more]
P24817	5.3e-33	38.37	Ribosome-inactivating protein beta-momorcharin OS=Momordica charantia OX=3673 GN...	[more]
P29339	2.0e-32	37.98	Ribosome-inactivating protein momordin II OS=Momordica balsamina OX=3672 PE=3 SV...	[more]
P84531	1.0e-28	37.92	Ribosome-inactivating protein cucurmosin OS=Cucurbita moschata OX=3662 PE=1 SV=2	[more]
Q00465	1.8e-25	35.39	Ribosome-inactivating protein luffin-alpha OS=Luffa aegyptiaca OX=3670 PE=1 SV=1	[more]

BLAST of ClCG08G004150 vs. ExPASy TrEMBL


Match Name	E-value	Identity	Description
A0A6J1EKL0	8.8e-71	56.36	rRNA N-glycosidase OS=Cucurbita moschata OX=3662 GN=LOC111433434 PE=3 SV=1	[more]
A0A6J1IUB2	4.3e-70	56.36	rRNA N-glycosidase OS=Cucurbita maxima OX=3661 GN=LOC111480641 PE=3 SV=1	[more]
A0A6J1E093	1.2e-59	51.70	rRNA N-glycosidase OS=Momordica charantia OX=3673 GN=LOC111024726 PE=3 SV=1	[more]
A0A6J1DVE7	1.1e-49	48.64	rRNA N-glycosidase OS=Momordica charantia OX=3673 GN=LOC111024729 PE=3 SV=1	[more]
A0A6J1DWK4	7.2e-49	47.67	rRNA N-glycosidase OS=Momordica charantia OX=3673 GN=LOC111024728 PE=3 SV=1	[more]

BLAST of ClCG08G004150 vs. ExPASy TrEMBL


Match Name	E-value	Identity	Description

InterPro

Analysis Name: InterPro Annotations of Watermelon (Charleston Gray) v2.5
Date Performed: 2022-01-31


IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
None	No IPR available	COILS	Coil	Coil	coord: 188..208
None	No IPR available	PANTHER	PTHR33453:SF14	RRNA N-GLYCOSIDASE	coord: 163..242 coord: 52..142
IPR017989	Ribosome-inactivating protein type 1/2	PRINTS	PR00396	SHIGARICIN	coord: 63..76 score: 42.67 coord: 111..126 score: 54.11
IPR016139	Ribosome-inactivating protein, subdomain 2	GENE3D	4.10.470.10	Ricin (A Subunit), domain 2	coord: 172..248 e-value: 3.6E-20 score: 73.7
IPR016138	Ribosome-inactivating protein, subdomain 1	GENE3D	3.40.420.10	Ricin (A subunit), domain 1	coord: 51..171 e-value: 6.4E-23 score: 83.2
IPR001574	Ribosome-inactivating protein	PFAM	PF00161	RIP	coord: 61..139 e-value: 3.4E-13 score: 49.7
IPR001574	Ribosome-inactivating protein	PANTHER	PTHR33453	FAMILY NOT NAMED	coord: 163..242 coord: 52..142
IPR036041	Ribosome-inactivating protein superfamily	SUPERFAMILY	56371	Ribosome inactivating proteins (RIP)	coord: 53..243

Relationships

The following mRNA feature(s) are a part of this gene:


Feature Name	Unique Name	Type
ClCG08G004150.2	ClCG08G004150.2	mRNA

GO Annotation

GO Assignments

This gene is annotated with the following GO terms.

Category	Term Accession	Term Name
biological_process	GO:0017148	negative regulation of translation
molecular_function	GO:0030598	rRNA N-glycosylase activity